LOCUS       DAZOZY010000001        70302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZOZY010000001 DAZOZY010000000
VERSION     DAZOZY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.001"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHURQ_000001"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHURQ_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR0821894.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHURQ_000002"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHURQ_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR0821895.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHURQ_000003"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHURQ_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR0821896.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHURQ_000004"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHURQ_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR0821897.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHURQ_000005"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHURQ_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR0821898.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHURQ_000006"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHURQ_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR0821899.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHURQ_000007"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHURQ_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR0821900.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHURQ_000008"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHURQ_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR0821901.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHURQ_000009"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHURQ_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHURQ_000010"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHURQ_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR0821902.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHURQ_000011"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHURQ_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR0821903.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHURQ_000012"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHURQ_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR0821904.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHURQ_000013"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHURQ_000013"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR0821905.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHURQ_000014"
     CDS             complement(14171..14743)
                     /locus_tag="ACHURQ_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR0821906.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHURQ_000015"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHURQ_000015"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR0821907.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHURQ_000016"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHURQ_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR0821908.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHURQ_000017"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHURQ_000017"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR0821909.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHURQ_000018"
     CDS             17912..18760
                     /locus_tag="ACHURQ_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR0821910.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHURQ_000019"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHURQ_000019"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR0821911.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHURQ_000020"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHURQ_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0821912.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHURQ_000021"
     CDS             complement(21672..21872)
                     /locus_tag="ACHURQ_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR0821913.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHURQ_000022"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHURQ_000022"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR0821914.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHURQ_000023"
     CDS             complement(23031..23387)
                     /locus_tag="ACHURQ_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR0821915.1"
                     /translation="MVTLYGIKNCDTVKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23769..23828
                     /gene="ypfM"
                     /locus_tag="ACHURQ_000024"
     CDS             23769..23828
                     /gene="ypfM"
                     /locus_tag="ACHURQ_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR0821916.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="ACHURQ_000025"
     CDS             complement(23927..27040)
                     /gene="acrD"
                     /locus_tag="ACHURQ_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR0821917.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="ACHURQ_000026"
     CDS             complement(27204..28904)
                     /gene="narQ"
                     /locus_tag="ACHURQ_000026"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR0821918.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29110..31089
                     /gene="aegA"
                     /locus_tag="ACHURQ_000027"
     CDS             29110..31089
                     /gene="aegA"
                     /locus_tag="ACHURQ_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR0821919.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31157..31732
                     /gene="nudK"
                     /locus_tag="ACHURQ_000028"
                     /pseudo
     CDS             31157..31732
                     /gene="nudK"
                     /locus_tag="ACHURQ_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            31858..32901
                     /locus_tag="ACHURQ_000029"
     CDS             31858..32901
                     /locus_tag="ACHURQ_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR0821920.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="ACHURQ_000030"
     CDS             complement(32997..35000)
                     /gene="tkt"
                     /locus_tag="ACHURQ_000030"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0821921.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35020..35970)
                     /gene="tal"
                     /locus_tag="ACHURQ_000031"
     CDS             complement(35020..35970)
                     /gene="tal"
                     /locus_tag="ACHURQ_000031"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0821922.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36259..38538
                     /gene="maeB"
                     /locus_tag="ACHURQ_000032"
     CDS             36259..38538
                     /gene="maeB"
                     /locus_tag="ACHURQ_000032"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR0821923.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38832..39167
                     /gene="eutS"
                     /locus_tag="ACHURQ_000033"
     CDS             38832..39167
                     /gene="eutS"
                     /locus_tag="ACHURQ_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR0821924.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39180..39659
                     /gene="eutP"
                     /locus_tag="ACHURQ_000034"
     CDS             39180..39659
                     /gene="eutP"
                     /locus_tag="ACHURQ_000034"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR0821925.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39634..40335
                     /gene="eutQ"
                     /locus_tag="ACHURQ_000035"
     CDS             39634..40335
                     /gene="eutQ"
                     /locus_tag="ACHURQ_000035"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR0821926.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40332..41135
                     /gene="eutT"
                     /locus_tag="ACHURQ_000036"
     CDS             40332..41135
                     /gene="eutT"
                     /locus_tag="ACHURQ_000036"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR0821927.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41132..42148
                     /gene="pta"
                     /locus_tag="ACHURQ_000037"
     CDS             41132..42148
                     /gene="pta"
                     /locus_tag="ACHURQ_000037"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0821928.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42187..42480
                     /gene="eutM"
                     /locus_tag="ACHURQ_000038"
     CDS             42187..42480
                     /gene="eutM"
                     /locus_tag="ACHURQ_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR0821929.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42587..42874
                     /gene="eutN"
                     /locus_tag="ACHURQ_000039"
     CDS             42587..42874
                     /gene="eutN"
                     /locus_tag="ACHURQ_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR0821930.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42886..44289
                     /locus_tag="ACHURQ_000040"
     CDS             42886..44289
                     /locus_tag="ACHURQ_000040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR0821931.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44300..45136
                     /gene="eutJ"
                     /locus_tag="ACHURQ_000041"
     CDS             44300..45136
                     /gene="eutJ"
                     /locus_tag="ACHURQ_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR0821932.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45126..46313
                     /gene="eutG"
                     /locus_tag="ACHURQ_000042"
     CDS             45126..46313
                     /gene="eutG"
                     /locus_tag="ACHURQ_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR0821933.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46630..47856
                     /gene="eutH"
                     /locus_tag="ACHURQ_000043"
     CDS             46630..47856
                     /gene="eutH"
                     /locus_tag="ACHURQ_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR0821934.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47853..49256
                     /gene="eutA"
                     /locus_tag="ACHURQ_000044"
     CDS             47853..49256
                     /gene="eutA"
                     /locus_tag="ACHURQ_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR0821935.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49268..50629
                     /gene="eutB"
                     /locus_tag="ACHURQ_000045"
     CDS             49268..50629
                     /gene="eutB"
                     /locus_tag="ACHURQ_000045"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR0821936.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            50650..51537
                     /gene="eutC"
                     /locus_tag="ACHURQ_000046"
     CDS             50650..51537
                     /gene="eutC"
                     /locus_tag="ACHURQ_000046"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR0821937.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51547..52206
                     /gene="eutL"
                     /locus_tag="ACHURQ_000047"
     CDS             51547..52206
                     /gene="eutL"
                     /locus_tag="ACHURQ_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR0821938.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52219..52719
                     /gene="eutK"
                     /locus_tag="ACHURQ_000048"
     CDS             52219..52719
                     /gene="eutK"
                     /locus_tag="ACHURQ_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR0821939.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52765..53817
                     /gene="eutR"
                     /locus_tag="ACHURQ_000049"
     CDS             52765..53817
                     /gene="eutR"
                     /locus_tag="ACHURQ_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR0821940.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="ACHURQ_000050"
     CDS             complement(53823..54722)
                     /gene="hemF"
                     /locus_tag="ACHURQ_000050"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR0821941.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="ACHURQ_000051"
     CDS             complement(54726..55595)
                     /gene="amiA"
                     /locus_tag="ACHURQ_000051"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR0821942.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55809..56234
                     /locus_tag="ACHURQ_000052"
     CDS             55809..56234
                     /locus_tag="ACHURQ_000052"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR0821943.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56221..56670
                     /locus_tag="ACHURQ_000053"
     CDS             56221..56670
                     /locus_tag="ACHURQ_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR0821944.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56731..57306
                     /locus_tag="ACHURQ_000054"
     CDS             56731..57306
                     /locus_tag="ACHURQ_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR0821945.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57402..58301
                     /gene="yfeX"
                     /locus_tag="ACHURQ_000055"
     CDS             57402..58301
                     /gene="yfeX"
                     /locus_tag="ACHURQ_000055"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR0821946.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="ACHURQ_000056"
     CDS             complement(58359..59663)
                     /gene="pbp4b"
                     /locus_tag="ACHURQ_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR0821947.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59668..61092)
                     /gene="murP"
                     /locus_tag="ACHURQ_000057"
                     /pseudo
     CDS             complement(59668..61092)
                     /gene="murP"
                     /locus_tag="ACHURQ_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="ACHURQ_000058"
     CDS             complement(61096..61992)
                     /gene="murQ"
                     /locus_tag="ACHURQ_000058"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR0821948.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62156..63013
                     /gene="murR"
                     /locus_tag="ACHURQ_000059"
     CDS             62156..63013
                     /gene="murR"
                     /locus_tag="ACHURQ_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR0821949.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63142..63933
                     /gene="ucpA"
                     /locus_tag="ACHURQ_000060"
     CDS             63142..63933
                     /gene="ucpA"
                     /locus_tag="ACHURQ_000060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR0821950.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64104..65120
                     /gene="cysP"
                     /locus_tag="ACHURQ_000061"
     CDS             64104..65120
                     /gene="cysP"
                     /locus_tag="ACHURQ_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR0821951.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65120..65953
                     /gene="cysT"
                     /locus_tag="ACHURQ_000062"
     CDS             65120..65953
                     /gene="cysT"
                     /locus_tag="ACHURQ_000062"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR0821952.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65953..66828
                     /gene="cysW"
                     /locus_tag="ACHURQ_000063"
     CDS             65953..66828
                     /gene="cysW"
                     /locus_tag="ACHURQ_000063"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR0821953.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66818..67915
                     /gene="cysA"
                     /locus_tag="ACHURQ_000064"
     CDS             66818..67915
                     /gene="cysA"
                     /locus_tag="ACHURQ_000064"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR0821954.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68049..68960
                     /gene="cysM"
                     /locus_tag="ACHURQ_000065"
     CDS             68049..68960
                     /gene="cysM"
                     /locus_tag="ACHURQ_000065"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR0821955.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68963..69331)
                     /locus_tag="ACHURQ_000066"
     CDS             complement(68963..69331)
                     /locus_tag="ACHURQ_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR0821956.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69436..70287
                     /gene="pdxK"
                     /locus_tag="ACHURQ_000067"
                     /gene_synonym="thiJ"
     CDS             69436..70287
                     /gene="pdxK"
                     /locus_tag="ACHURQ_000067"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR0821957.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16702 a        18357 c        19435 g        15808 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgatggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa cggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataaata ctcatttaag ggtgaaaaca ttttgcctgc aaatgattat
    23461 caggaaatat aacattatcg aagtattcaa attaacaact attaaataat attaaacatt
    23521 tttaacgtaa acattaagaa tattcatctt gttatctgtc tgtattttaa cctgaattaa
    23581 atcagtatta gatgccgtat gaatatctcg aaatgttgct gtgtatcaca tacaaatcat
    23641 caccgcagga ccatagtatt tacataagat ttgtcccccc ggaggtagtc atcgcagcaa
    23701 tcgagtaaat tagcttcacc acggtatatt gtttccgtag aatacccata attataagag
    23761 aggttgttat gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc
    23821 gtaagtaatt cctgagacaa agtataaaca gcaagaaccc gctgagcagg ttcttaatcg
    23881 gaaagttgaa aaaggcgaca cattggcatg tcgccttttt tattgcttat tccgggcgcg
    23941 gctttagcgg gaagcggcgg cgcaccagca caaagaacag cggcacgaag taaatagcca
    24001 gaatagtggc tgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg
    24061 aaccggcacc cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca
    24121 tcagaatcgg gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc
    24181 ctttttggtt catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac
    24241 caatgaccgt taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg
    24301 ttgccagcag cgcgccgatt acccccagcg gcacgaccag cattaccgag aacggcaccg
    24361 accagctttc atacaacgca gccagacaca ggaataccac cagcaaggaa atggcgtaca
    24421 gcgccggagc ctgcgcgccg gaaagtcgct cctgatacga catcgccgtc cactccagac
    24481 caaagccgtt tggcagctgc ttcactaacg attccataat atccatcgcc gtaccggtac
    24541 tgacccccgg tgcggcttcc ccaacaatct caaccgcaga ataaccgtta tagcgttcca
    24601 gacgcggcga gcctgtttcc cagcgtgagg tcgcgaaagc agagaagggc accatgccgc
    24661 catctttatt tcggacatac cagagattga tgtcatctgg cagcatgcga tacggcgcag
    24721 ctgcctgcac atagactttc ttcacgcgac cgcgatccat aaagtcattc acatagctcg
    24781 aaccccaggc ggtttgcagt gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag
    24841 ctttacgctg gtcgatatca atctgcaact gcggactgtc gtcgaggccg ttatggcgca
    24901 cacgggttag ctccgggttt tccgccgcca gcgccagcaa ctggttacgt gctgccatca
    24961 gcgcatcgtg acctgctcca gcgtggtcct gcaactccat atcaaaaccc gcagaactac
    25021 caagaccgct aattgctggc gggctgctgg cgataacgcg agcttcttta atcttgttaa
    25081 acgcctttgt tgcacgctca ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc
    25141 tccagtcttt caggcggata aacattcgcg ccacgttttg cccgttaccc ccagggccag
    25201 aaccaacggt ggcaaacacc gacatgatgt tgtctttttc atgggtgaag taatatttct
    25261 cgatttgctc aacgactttc agggtctgtt gttgggttga accgctgggc aattgtaccg
    25321 aggtagtaaa catgccacgg tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca
    25381 ggaacaccat gccgccaagc agcaggacat aaatcacaat ccagcgcagg ctgcggtgga
    25441 gaattttcgc cacccctttt tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc
    25501 aggcaaaaaa gcctttttgc ccgtgatgct cacctttttt cagtggctta agtagtgtgg
    25561 cacacagagc cggagtgagg atcatcgcta ccagtactga cagcaccatc gccgcaacaa
    25621 tggtaataga gaactggcga tagatggcac cggtggtgcc gccgaagaag gccattggta
    25681 caaataccgc cgaaagcacc atcgcaatcc cgaccagtgc cccctggatc tgccccatcg
    25741 atttacgtgt ggcttcgcga ggagtgagtc cttcctcact cataatacgt tcgacgtttt
    25801 ccaccaccac gattgcgtca tccaccagca gaccgatcgc cagtaccatc gcgaacatgg
    25861 ttaaggtgtt gacgctgtaa ccgaaggcgt aaagtacgga gaaggttccc atcaacacca
    25921 ccggcacggc gatagtgggt atcagcgtgg cgcggaagtt ttgcaggaac agatacataa
    25981 cgaggaaaac cagggcgata gcttccagca gcgttttcac cacgtcttca atggaggctt
    26041 taacaaacga ggtggtttca tacgccacct tgtattccag tccgtgcggg aagtactgcg
    26101 ccagctcgtc gagacgattg agcaccagct ccgctgtcgc catttcgttt gcgccggagg
    26161 ccagttttac ccccagcccg gaggctggct taccattgaa gcggctaaga taatcgtatt
    26221 tctccgcccc catttcgacg gtggcgacat cgcccagcct tacctctgag ccgtcttgat
    26281 tcacccgcaa ggtgatatcg cggaactgtt ctggtgtttg cagcagtgac tgggcgttaa
    26341 tggtggcgtt gagcgcctgc ttatcgacgg aaggtgtacc accaagttgc ccaaccgcaa
    26401 tctgcgcgtt ctgtgactca atggcatcag tgacatcttt agccgtcatc tggaaactgt
    26461 tgagtttcgc cgggtccagc cagatacgca tggaatattg cgaaccatag gcatcgatat
    26521 ccccgacgcc attcacgcgg cttaacgggt cctgaatatt actggcaaca taatcggcaa
    26581 tatcctgttt atccatcgaa ccatcggtag agacgaaagc aatggtcaga atattggtat
    26641 cgccggtttt acgcaccgtc acgccctgat tttgcaccgc ctgcggtaac tttcgcatgg
    26701 ctgattgcag ctggttttgt acttgctgca cggcttcgtc cggatcggtg cctgctttaa
    26761 aacttaaagt gacagatgcc tgaccgatgc cactgctctg agatgacata tacatgagat
    26821 tatcgaggcc ggtcatattt tgctcgataa cctgggtcac ggtgttttcc agcgtctggg
    26881 ccgatgcgcc gggatagtta gcggtcactc gcacattcgg tggcgcgaga tcggggtatt
    26941 gttcaacggg caatgaaaaa atcgccaggg tccctgtcag acacaacagg attgccagca
    27001 cccaggcaaa aatggggcga tcaataaaga aattcgccat taaaagagga cctcgtgttt
    27061 ctgcgtatcg ttgtaggctt gacttgcttc actgtagcgg caaggtacga gccaatcgtg
    27121 gagaaaaaaa ggagttaatg taaattatca tgccctttta tactgaacct taagtgcaag
    27181 tattctttgg tcagtaggag gcattacatc aactgacttt cctcaccctc cgcagagcga
    27241 aagctaatac tcactaacgt gccgccgccg gaaggttgtg aaaaagtcag cgtcccaccc
    27301 agccgttccg cgcgttcgcg catgatattc agaccataat gaccttcggg ttctttcggt
    27361 tcaccgatac caatcccgtt atcacggata taaaccgtgt gattgccgtc cggcgcggtg
    27421 acgcaactga cggcgatttc gctggcgttg gcgtgcttca tcgcattcag caccgcttcg
    27481 cgaataattt gcaacaaatg cacctgcatt tgcgcatcca gcgccagggt tggcagacgg
    27541 cagtcgaggg tcagtttggc gctggtttga ttttgtaacg tatccagcat ttccctcaac
    27601 gcggagggga gatccgcctg ctgcagcgtc aggcggaaag tagtcaacag ctcgcgtaac
    27661 tgccgataag catcattcaa cgcctgggaa aaatcggcca tgatactttg tgcggtggcg
    27721 ttatcttccg gtatcgaacg cttcagtaac gtcaactgga tacgtaagta agaaagtacc
    27781 tgagccagcg agtcgtgcaa ttcgcgggcg atggttgcac gttcttccat caacaataac
    27841 tgctgaaaat gcttttgcgc ctgattaaag tacaaaccgc gtcccagcat cgacgaaacg
    27901 ctgttaagca gcggttctga tgatgaaacg tgactatttt gccagtgcag ttcgccgtaa
    27961 accgtctctt gcattgtcac cggtaaaatc tgcatcggca attccgggtt tggttgccct
    28021 tcgctaatcc gccagttttc accgacattt aactccagat attcagccgc ttcattgtcg
    28081 cgaacaatct gcaaaatatg gcggaaacaa tgcacatcaa tctggcttgt gttcagcgcc
    28141 tgcgaacatt gatacaacac ctccagacga cgcttggcct cgtggagatc gcgggtcttt
    28201 tcttctactg acgcctccag cgaaaggtac aatttatgca gctcgctcga catctggtta
    28261 aaggtttttg caagcagacc aagctcattc ggcagactgg tatccagcgg cggcgagtcg
    28321 aactgcccgt gttcaatacg ttgactggcg gtaaccagct gattcagcgg ggcaaccacc
    28381 tgatggcgta tacggcgcaa agtaaaaaag accagcgtga aaataccgat accgccagcc
    28441 agggaaatcg ccaccaccag cagcattttg cgttcagcgt agtgctgtaa agccagcacg
    28501 aacaggtcta tctgattaac ataattatta atattggcct gataccacgg caaatcgccc
    28561 ttgctgagcc gattattcat ctccagccag ttggcattca gatgcgcata gcgagttttt
    28621 actgcttctg gcacatacca gacgttgaga ttggttaata ccgatgaatg cagtgcctgc
    28681 tgaaacaact ggcgatgtgc attgagttgt ggactgccac tttgcaagtc gtagcccagg
    28741 cggtaactct gcatacgcag cgatccggca atattgatag cctcagcgtc gcgcaaactg
    28801 ctcgccagag ttagcagagc gatacccgtg gaaagaatcg acagcagcac aatgtaaaaa
    28861 aaggcccggg ccagactggc cgagacgggt cgtttaacaa tcacacgcgt cttctccaca
    28921 aaaaatcatt aatgttccag ttcgctaaaa cgcaatttag tcagtgatgg gattatttga
    28981 tctgccacag tttatagcga aatagttact tattctgggc acatgaacat tgccgattcc
    29041 cctgtaagtc gggtaataac aacactcata taaagaataa ggtttttaca accaaaaaag
    29101 aaggtcgtta tgaatcgttt tattatggcc aacagtcagc aatgtctggg ttgtcatgct
    29161 tgtgaaatcg cctgtgtcat ggctcacaat gatgagcaac atgtcctgag ccaacaccat
    29221 tttcatcccc gaattacggt tatcaaacat cagcagcaac gtagtgcagt gacctgtcac
    29281 cattgtgaag atgcgccctg cgcccgtagc tgccctaatg gcgcaatcag ccacgttgat
    29341 gacagcattc aggtcaatca gcaaaagtgt attggctgta aatcctgcgt ggtggcctgt
    29401 ccgtttggta cgatgcaaat cgtcctgaca cccgtcgcgg caggaaaagt aaaagccacg
    29461 gcgcataaat gcgacctttg tgcagggcgc gaaaacggtc ctgcctgtgt cgagaattgc
    29521 ccggcggacg cgctgcaact ggtcactgac gtcgcactct ccggcatggc gaaatcccgc
    29581 cgcttgcgca ccgcacgtca ggagcatcaa ccgtggcatg ccagtaccgc tgcacaagaa
    29641 atgccggtaa tgagtaaagt cgaacaaatg caggcaacgc ccgcgcgtgg cgagccggat
    29701 aaactggcga ttgaagcgcg caaaaccggt tttgatgaaa tttatctgcc attccgtgcc
    29761 gatcaggcac aacgggaagc ctcgcgctgc cttaaatgcg gcgagcacag cgtttgtgaa
    29821 tggacctgcc cgctgcataa ccatattccg cagtggattg aactggtgaa agccggaaac
    29881 atcgacgccg ccgtcgagct ttctcacctg accaacaccc tgccggaaat taccggacgc
    29941 gtttgtccgc aggaccgttt gtgtgaaggt gcctgtacta ttcgcgatga gcacggcgcg
    30001 gtaactatcg gcaacattga acgctacatt tcagatcagg cgttggcgaa aggttggcgt
    30061 cctgacttaa gccatgtcac caaagtggac aagcgggtgg cgattatcgg tgcgggtccg
    30121 gcagggctgg cctgtgcgga tgttctgacc cgcaatggcg tggcggtgac ggtgtacgat
    30181 cgccatccag aaattggtgg cttgctcact ttcggcattc cttctttcaa actggataaa
    30241 tccctgctgg cacgccgtcg ggaaatcttc agcgcgatgg ggattcactt cgaactcaat
    30301 tgtgaagtgg gtaaagatgt ctctttggat tcgcttttgg aacaatacga cgcggtcttc
    30361 gttggcgtag gcacttaccg ttccatgaaa gcgggtttac ccaatgaaga tgcgccgggc
    30421 gtttatgacg cgctgccgtt cctcatcgcc aacactaaac aggtgatggg actcgaagag
    30481 ctaccggaag agccgtttat caataccgcc ggactcaacg tcgtggtact gggcggcggc
    30541 gacaccgcga tggactgtgt gcgtaccgcg ctgcgccacg gcgcgagtaa cgtcacctgc
    30601 gcttatcgtc gtgatgaagc taacatgcca ggctcgaaga aagaggtgaa gaacgcccgc
    30661 gaagaggggg ccaacttcga atttaacgtc cagccggtgg cgcttgagct gaatgagcaa
    30721 ggtcacgttt gcggaattcg tttcctgcgc acgcgtcttg gcgagccgga tgcccagggg
    30781 cgccggcgac ttgtaccgat tgagggcagt gaatttgtca tgccagccga tgcggtgatt
    30841 atggcgtttg gcttcaatcc acacgggatg ccgtggctgg agtcgcacgg tgtaacggta
    30901 gacaaatggg gccgcatcat cgcggatgtg gaaagccagt accgttacca gaccaccaat
    30961 ccgaaaatct tcgccggtgg cgacgccgtg cgtggtgcgg atctggtggt taccgcaatg
    31021 gcagaaggac gtcatgcggc acaggggatt attgactggc tgggggtaaa atcagtcaaa
    31081 tctcactgat agcctgcgca gacaaacccg acttcacagc gtaatataat tgttcatttc
    31141 gcgctgtgga gtcggtatga cgcaacaaat caccctcatt aaagacaaaa ttctctccga
    31201 taactatttc accctgcaca acattactta cgatctcacg cgcaaagatg gctaagttat
    31261 ccgccataaa cgtgaagttt acgatcgcgg taatggtgcg acgatcctcc tgtacaacgc
    31321 gaagaaaaag accgtggttc tgattcgtca gttccgtgtc gctacctggg ttaatggcaa
    31381 tgaaagcggg cagctgattg aaacctgcgc cgggctgctg gataacgacg aaccggaagt
    31441 gtgcattcgt aaagaagcga ttgaagagac gggctatgaa gtgggcgaag tgcgcaaatt
    31501 atttgaactg tatatgtcgc ctggcggtgt gactgagcta atccactttt ttatcgccga
    31561 atacagtgac aatcagcgcg ctaacgccgg tggcggtgtt gaagatgaag atattgaagt
    31621 gctcgagctg ccgttcagcc aggcgctgga gatgatcaaa accggcgaga tacgtgacgg
    31681 taagacggtg ttattgctta actatttgca aacgtcacat ttaatggact gaaaaataac
    31741 aataatattt cgttgtttat tattggctca atccgacaaa tctgattgag ccgcgctacc
    31801 tgcgcaacga agatacgact tgtgctgttt gtttgaactt ctggggtcgt accgtccatg
    31861 cgctatcgca ttttccttct cttttttttc gctttgttgc cgacgtcttt ggtgtgggcg
    31921 gcactagcgc aacgggcgtt ttccgactgg caggtcacct gtaataacca aaacttctgc
    31981 gtggcgcgca atacgggcga tcataatgga ctggtgatga ccctgagccg cagcgccggg
    32041 gcgcataccg atgccgtttt acgtattgag cgcggcgggt tgaagtcgcc ggatgcgtct
    32101 gaaggagaga tagcgccacg gctgctgtta gatggtgagc cgttggcgtt aagtggtgac
    32161 aagtggcgga tttcaccatg gttattagta accgatgata cggcaaccat caccgcgttt
    32221 ttgcagatga ttcaggaagg gaaggcgatc accttacgcg acggcaatca gaccatttct
    32281 ctgagtggct taaaagcggc gttgttgttt attgatgctc agcaaaagcg cgttggcagt
    32341 gaaaccgcgt ggatcaagaa aggggacgaa ccgccgctca gcgtaccgcc cgcgcctgcg
    32401 ctgaaagagg tcgcggtggt taacccaacg ccgacgccac tctcactcga agaacgcaac
    32461 gatttgctgg attatggaaa ctggcggatg aatggtctgc gctgctcgct tgatccgtta
    32521 cgtcgtgagg tgaatgtcac tgcgctgact gatgataaag cgctgatgat gatcagctgt
    32581 gaggcagggg cctataacac cattgatttg gcatggattg tgtcgcgtaa aaagccgcta
    32641 gcctcgcgcc cggttcggtt gcgtttgccg ttcaacaacg ggcaggagac gaatgaactg
    32701 gaactgatga acgcaacatt tgatgagaaa tcgcgtgaac tggtgacctt agcgaaaggg
    32761 cgcggattaa gcgattgtgg cattcaggcg cgctggcgct ttgacggtca gcgttttcgc
    32821 ctggtgcgtt acgccgcaga acccacctgc gataactggc atgggccaga cgcctggccc
    32881 acgttgtgga tcacccgtta aacaaaatgg cggggacatg taggccggat aagacgctgt
    32941 aggccggata agacgcgtca gcgtcgcatc cggcaatcag catccggcga tcactatcag
    33001 gcacctttca ctcccagcac cttatgcgct tttgccacaa tattctcggc ggtaaagcca
    33061 aagaacggga acagcttatc cgccggagca gattccccat aacccgtcat cccgacaatt
    33121 gcccctttca gaccaacata cttgtaccag taatcggcaa tacctgcttc caccgccacg
    33181 cgagccgcaa cgttagaagg caacaccgac tcccgatatt cctcatcctg ggcgtcgaaa
    33241 atatcggtcg agggcaggga aaccacgcga acattgcgac cttctcctgc taatttctcc
    33301 gcagcttgca gggtgatttc catctctgaa ccggtggcaa tcagaataat atctggctta
    33361 ccgccgctgt ctttcagcac ataaccgcca cgagtaatct ctttaacctg atccggcgta
    33421 cgttccacct gggccagatt ctgccttgag aggatcagtg ccgtcggtcc gttgtggcgc
    33481 tcaaccgcca gcttccagcc caccgccgct tccacctgat cgcacggtcg ccaggtgctg
    33541 aaatttggcg ttaagcgcag gctggccagt tgctcaacag cctggtgcgt cggaccatct
    33601 tcgcccaggc caattgagtc gtgggtataa accataatct gccgcgcttt catcagtgcc
    33661 gccatccgcg cggcgttacg ggcgtattca acaaacatca ggaacgtcgc ggtatacggc
    33721 acaaagccgc cgtggtgcgc gatgccgttg gcgatagcgg tcatgccaaa ttcacgcacc
    33781 ccgtagtgaa tgtagttgcc cgccggatct tccttcagcg aaacagaacc tttccagatg
    33841 gtcaggttgc tgggagccag atccgccgaa ccgccgagca gctccggtag caaggggcca
    33901 taagcgttaa gcgtattttg cgaagcctta cgggtggcga ttttcgccgg attcgcctgc
    33961 aactcattga tatacttctg agtcgttttc tcccagtcct tcggtaaacc accgctcatc
    34021 cgacgggtaa actcttctgc cagttgcgga tgagcctttt tataggcggc aaacttctca
    34081 ttccacctct gctgcgcttt ttcgcctttt tcgcgggcat cccaggcgtg ataaatatct
    34141 ttagggatct caaatggcgg atggtgccag cccagttttt gccgtgccag cgccacttct
    34201 tcttccccca gcggtgcgcc gtgcgcctct tccttacctg ctttattcgg cgatccaaag
    34261 ccaatcaccg tacgacagat aatcagcgac ggcttatctt tcacgctttg cgcttcaagg
    34321 atcgcttcct tcaccgcctg cggatcgtga ccgtcgattt catggatcac atgccagtga
    34381 taggcttcaa aacgttttgc cgtatcgtcg gtaaaccagc cttctgtttc accgtcgatg
    34441 gaaataccgt tgtgatcgta aaaaccaatc agcttgccca gtcccagcgt gcctgccagc
    34501 gaacagactt cgtgggaaat accttccatc aggcagccgt cgcccataaa cacataggtg
    34561 aagtgatcga cgatctcatg gtctggctgg ttaaactgcg ccgccagtgt acgctctgct
    34621 atcgccagcc cgacggcgtt cgccaaacct tgtccaagtg gaccggtggt ggtttcaacg
    34681 cctggcgtat aaccaatctc cgggtggcct ggggttttcg aatgcagttg acgaaagttc
    34741 ttcagctctt ccagcggcag gtcgtaaccg gtcagatgca gcaaactgta gagcagcatc
    34801 gacgcgtgac cgttggaaag aataaagcgg tcgcgatcat accaggttgg gtcggtaggg
    34861 ttatgtttaa gaaaatcgtt ccacagcact tcggcaatat cagccatgcc catcggcgcg
    34921 ccgggatgac cagaattggc tttttgtacc gcatccatac tgagtgcgcg aatcgcattg
    34981 gcaaggtctt ttcgggacat ataacactcc gtggctggtt tatagtttgg cggcaagaag
    35041 atcttccagt ttgcgttgat caacggcgaa cagacgaatg ccttcagaca gtttttctac
    35101 cgccatcgca tactgattgt gctcccaacg gaactccgct tcgctcatgg gagctgggcg
    35161 tgggaacgtc tgagaaggtg ggattaattt acgtaccact ggcgaaactt tttcctgcag
    35221 ctccttcagc aaattcggtg cgatagtcag tcgatcgcag ccggttaagg cgaggatttg
    35281 ttcggtgcga cggaagctcg cgcccatcac aatggtttca tagtggtgtt gcttatagta
    35341 gtcgtagata ttgcgcaccg atttaacgcc cggatcttct tccaccacat acgggtccat
    35401 cggcttgcgt gcctgatacc agtcataaat acgcccgaca aacggcgaaa tcagaaaaac
    35461 gcctgcttcc gcacaggccc gtgcctgtgc aaaagaaaac agcagcgtca ggttgcagtt
    35521 aataccttct ttttccagct cttctgccgc gcgaattcct tcccaggtcg aagccagctt
    35581 gatcagaatg cgtgatttct caacgccttg ttgctgatac aagtccacca gatggcgcgc
    35641 cttctcaata ctcttttctt tatcaaaaga gaggcgtgca tcaacttctg ttgacacgcg
    35701 accgggtacg attttgagga tttcagcacc gaaattgacc gccagtttgt cacacgctgc
    35761 gaccacctgt tgttcctggg tcttgccatt ttttttaccc caggcgatag catcgtctat
    35821 taaatgctca tattgtgata atccggcagc cttgagtaac agcgaaggat tggtggtggc
    35881 atcctgggga tgataatggc gaatggactc aatatcgccg ctgtctgcca cgacagtggt
    35941 gaactgtttg atgccgtcta actcgttcat aggaaatact ccttgaaaag taaagtgtta
    36001 gatgagtgcg ttaattcaca cttctgagaa atttcgctaa acgcatcaaa aaagcatagc
    36061 agacaggcat ggtattgctg gattaagcag gtaacatcag tgttatagga ttattaccaa
    36121 aacattatat taatggtgag agtttggact tgctcaaagt ctgtagactc cggcaaggta
    36181 ataatgtgcg ccacgttgtg ggcagggggt gttgcccaca cactttattt gtgaacgtta
    36241 cgtgaaagga acaaccaaat ggatgaccag ttaaaacaaa gtgcacttga tttccatgaa
    36301 tttccagttc cagggaaaat ccaggtttct ccaaccaagc ctctggcaac gcagcgcgat
    36361 ctggcgctgg cctactcacc cggcgttgcc gcaccttgtc ttgaaatcga aaaagacccg
    36421 ttaaaagcct acaaatatac cgcccgcggt aacctggtgg cggtgatctc taacggtacg
    36481 gcggtgctgg ggttaggcaa cattggcgcg ctggctggca aaccggtgat ggaaggcaag
    36541 ggcgttctgt ttaagaaatt cgccgggatt gatgtatttg acattgaagt tgatgaactc
    36601 gacccggaca aatttattga agttgtcgct gcgctcgaac cgaccttcgg cggaatcaac
    36661 ctcgaagaca ttaaagcgcc agaatgtttc tatattgaac agaaactgcg cgagcggatg
    36721 aatattccgg tattccacga cgatcagcac ggcacggcaa tcatcagcac tgccgccatc
    36781 ctcaacggct tgcgcgtggt ggagaaaaac atctccgacg tgcggatggt ggtttccggc
    36841 gcgggtgccg cagcaatcgc ctgtatgaat ctgctggtag cgctgggtct gcaaaaacat
    36901 aacatcgtgg tttgcgattc aaaaggcgtt atctatcaag gccgtgagcc aaacatggcg
    36961 gaaaccaaag ccgcatatgc ggtggtggat gacggcaaac gtaccctcga tgatgtgatt
    37021 gaaggtgcgg atattttcct gggctgttcc ggcccgaaag tgctgaccca ggaaatggtg
    37081 aagaaaatgg ctcgggcacc aatgattctg gcgctggcga atccggaacc ggaaattctg
    37141 ccgccgctgg cgaaagaagt gcgtccggat gccatcatct gcaccggtcg ttctgactat
    37201 ccaaaccagg tcaacaacgt cctttgcttc ccgttcatct tccgtggcgc gctggacgtt
    37261 ggcgcaaccg ccatcaacga agagatgaaa ctggcggcgg tacgcgcgat tgcagaactg
    37321 gcccatgcgg aacagagcga agtggtggct tcagcgtatg gcgatcagga tctgagcttt
    37381 ggtccggaat acatcattcc aaaaccgttt gatccgcgct tgatcgttaa gatcgctcct
    37441 gcggtcgcga aagcggcgat ggagtcgggc gtggcgacgc gtccgattgc tgatttcgac
    37501 gtctacatcg acaagctgac tgagttcgtt tacaaaacca acctgtttat gaagccgatt
    37561 ttctcccagg ctcgcaaagc gccgaagcgc gtagttctgc cggaagggga agaggcgcgc
    37621 gttctgcatg ccactcagga actggtaacg ctgggactgg cgaaaccgat ccttatcggt
    37681 cgtccgaacg tgatcgaaat gcgcattcag aaactgggct tgcagatcaa agcgggcgtt
    37741 gattttgaga tcgtcaataa cgaatccgat ccgcgcttta aagagtactg gaccgaatac
    37801 ttccagatca tgaagcgtcg cggcgtcact caggaacagg cgcagcgggc gctgatcagt
    37861 aacccgacag tgatcggcgc gatcatggtt cagcgtggcg aagccgatgc aatgatttgc
    37921 ggtacggtgg gtgattatca tgaacatttt agcgtggtga aaaatgtctt tggttatcgc
    37981 gatggcgttc acaccgcagg tgcaatgaac gcgctgctgc tgccgagtgg taacaccttt
    38041 attgccgata cctacgtcaa tgacgaacca gacgcagaag agctggcgga gatcaccttg
    38101 atggcggcag aaactgtccg tcgtttcggt attgaaccgc gcgttgctct gttgtcgcac
    38161 tccaactttg gttcttctga ctgcccgtcg tcgagcaaaa tgcgtcaggc gctggaactg
    38221 gtcagggaac gtgcgccaga actgatgatt gatggtgaaa tgcacggcga tgcggcgctg
    38281 gtggaagcga ttcgcaacga ccgtatgccg gacagccctt tgaaaggttc tgccaatatt
    38341 ctggtgatgc cgaacatgga agctgcccgc attagttaca acttactgcg cgtttccagc
    38401 tcggaaggtg tgactgtcgg tccggtgctg atgggcgtgg cgaaaccggt tcacgtgtta
    38461 acgccgatcg catcggtgcg tcgtatcgtc aacatggtgg cgctggccgt ggttgaagcg
    38521 caaacccaac cgctgtaatt cttttttaac tctcacgctt accctgaata ttcagggtaa
    38581 gcggtttggc tgcaatatat tagtaaagct tattactgag tttgcgaata ataaaaaaaa
    38641 gcagtctata taatatctcg atattatttt atttatattc atgcgttgca tatgaaagtt
    38701 tatgcaccac agcgaatatc tctccattct tagtgatcta cctcaccttt taaacgcgct
    38761 tgccgaattt tgttatttac tctgacgaaa aattgtcacg atacacgaaa gtttttcaca
    38821 ggcggcgact catggataaa gaacgcatca ttcaggaatt tgtgcccggc aaacaggtca
    38881 cactggcgca tctcatcgcg catcccggtg aggaactggc gaagaaaatc ggcgttccgg
    38941 atgcgggcgc aattggcatt atgacgctaa ctcccggcga aacggcgatg atcgtcggtg
    39001 atttagcgct gaaagccgct gatgtgcata tcggttttct cgaccgcttc agcggtgcat
    39061 tggtgattta tggctcggtg ggcgcagtag aagaagcgtt atcacaaacc gtcagcggtt
    39121 taggtcgctt attaaattat acgctttgtg aaatgaccaa aagttaatac gaggcattca
    39181 tgaaacgaat tgcgtttgtg ggtagcgtcg gggcaggaaa aacaacgtta tttaatgcgt
    39241 tgcaggggaa ttataccctc gccagaaaaa cacaggccgt ggaatttaat gataagggcg
    39301 atattgatac gccgggtgaa tattttagcc atccccgctg gtatcacgcc ttaattacca
    39361 cgctacagga tgtagacatg ctgatctatg tccatggcgc aaatgacccg gaaagtcggt
    39421 tgcctgccgg gttgctggat attggcgtca gcaaacggca aatcgccgtc atcagtaaaa
    39481 cggacatgcc agatgctgac gtcgccgcaa cgcgaaagtt attgcttgag acgggtttcg
    39541 aagagccgat tttcgagcta aacagccacg acccgcaaag cgtgcagcaa ctggtggatt
    39601 atctggcatc tctcaccaaa caggaggaag caggtgaaaa aactcatcac agcgaatgat
    39661 attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg tggttctgcg cgccagcatt
    39721 attaccccgg aagcccgtaa agtggcggat ctactgggtt ttaccatcac cgaatgtgat
    39781 gaatcgatcc cggtaacggc gtccgtgccc gccagcgtgc cagccgacaa aaccgaaagc
    39841 cagcgcatcc gcgaaaccat cattgcccag ctgccggaag gccagtttac cgaaagcctg
    39901 gtcgcacagc tgatggaaaa agtgatgaag gaaaaacagt cgctggagca gggcgcaatg
    39961 cagccgagct tcaaatcggt gaccggcaaa ggcggcatca aagtgattga tggcagcagc
    40021 gtcaagtttg gtcgttttga tggcgcacaa ccgcattgcg tgggcttgac tgatcttgtg
    40081 accggagacg acggcagcag catggccgcc ggttttatgc agtgggagaa cgcattcttc
    40141 ccgtggaccc tgaactacga cgaaatcgac atggtgctgg aaggggagct ccatgttcgc
    40201 cacgaaggcg aaaccatgat cgccaaagca ggcgacgtga tgtttatccc gaaaggctcc
    40261 agcatcgaat ttggtacgac atccagcgtg aaattcctgt atgtcgcctg gccggctaac
    40321 tggcaatccc tatgaaagat ttcatcaccg aagcatggct aagagcgaac catacgctca
    40381 gcgaaggcgc agagatccat ctccctgcgg acagtcgcct gacgccctct gcccgggagt
    40441 tgctggaaag ccgccatctg cgcatcaagt ttattgacga gcagggccgc ctgtttgttg
    40501 acgatgaaca gcagcagccg cagcccgttc atgggctgac cagtagcgat gaacatccgc
    40561 aggcgtgctg cgaactgtgc cgccagccgg tggcaaaaaa gccggatacg ctgactcacc
    40621 tgtcggcgga aaaaatggtc gccaaaagcg atccgcgtct gggttttcgc gcggttctcg
    40681 acagcaccat tgcgctggca gtgtggctgc aaattgaact ggcggaaccg tggcagccgt
    40741 ggctggcgga tatccgttcg cgtcttggca acattatgcg cgccgatgcg ctgggggaac
    40801 cgctgggctg ccaggcgatt gtcgggcttt ctgacgaaga tctgcatcga ctttctcacc
    40861 agccgctgcg ctatctcgac cacgatcatc tggtgccgga agccagccac gggcgcgatg
    40921 cggcgttgct caatctgcta cgtaccaaag tgcgggaaac cgaaaccgta gcggcgcagg
    40981 tgtttatcac ccgcagcttt gaagtattac gcccggacat cttgcaggcg ctgaaccgcc
    41041 tttcgagcac ggtctacgta atgatgatcc tgagcgtaac gaagccgccg ctaacggtga
    41101 agcagatcca acaacgactg ggggaaacgc aatgattatt gaacgttgtc gtgaactggc
    41161 gttgcgagcg cccgccagag tggtttttcc ggatgcgtta gatcaacgtg tgctgaaagc
    41221 tgcgcaatat ttacatcaac aaggtctggc aacgcccatt ctggtcgcta atccgtttga
    41281 acttcgtcag tttgcgctca gtcacggcgt ggcgatggac gggctacagg tgattgatcc
    41341 gcatggcaac ctcgcaatgc gggaagaatt tgctcatcgc tggctggccc gcgcgggcga
    41401 aaaaacgccg ccggatgcgc tggaaaaact taccgatccg ctgatgttcg ccgcagcaat
    41461 ggtcagcgcc ggtaaagcgg atgtctgtat cgcgggcaac ctctcttcca cggcgaatgt
    41521 gctgcgtgcc ggattacgta ttatcggctt gcagccaggc tgtaaaacgc tctcatccat
    41581 tttcctgatg ctgccacagt acagcggtcc ggcgttgggc tttgccgatt gcagcgtggt
    41641 gccacagccg acggcggcgc aactggcgga tatcgcgctt gccagtgccg aaacctggcg
    41701 cgccctcacc ggagaagagc cgcgcgtggc gatgctgtcg ttttccagta acggtagcgc
    41761 ccgtcacccc tgcgttgcca acgtccagca ggcgacagaa atcgtccgtg agcgcgcacc
    41821 aaagctggtg gttgatggcg agttgcagtt tgacgccgcc ttcgtgccgg aagtggcggc
    41881 gcaaaaagcg cctgccagcc cgctacaggg caaagccaat gtgatggttt ttccgtcgct
    41941 ggaagccgga aatattggct acaaaatcgc acaacgactc ggcggatatc gtgccgtcgg
    42001 gccattgata caaggacttg ccgcgccgat gcacgatctc tctcgtggtt gtagcgtgca
    42061 ggaaattatc gagctggcgc tggtggcagc tgtgccgcgt cagacagaag tgaaccgcga
    42121 aagcagttta caaacactgg ttgaatgaac ggtcccgttc tggaccctga taaagaggaa
    42181 agcacaatgg aagcattagg aatgatcgaa acccggggcc tggttgcact gattgaggct
    42241 tctgacgcga tggttaaagc tgcgcgcgtc aagctggttg gtgttaagca gattggcggc
    42301 ggcctgtgta ctgccatggt tcgtggtgat gttgcagcct gtaaagccgc gaccgatgca
    42361 ggtgccgcag cggcacagcg tatcggcgag ctggtatctg tacacgttat cccgcgtccg
    42421 cacggcgacc tggaagaagt cttcccgatc ggcctgaaag gcgatagcag caacctgtaa
    42481 tcaccctgtg tgacgtcgat tcgggcgtca cgctctctcc tttttcattt taccttctgc
    42541 ggagggtagg gggagctttt acctgaaata cgccacggag gcgggtatga aactggcagt
    42601 cgtcactgga caaattgttt gtaccgtacg ccatcacgga ctggcgcatg acaaattgct
    42661 gatggtggaa atgattgatc cacaaggtaa tcctgacggg caatgcgccg tcgccatcga
    42721 caatattggc gcgggaaccg gggagtgggt gttactggtg agtggcagtt ccgcccgcca
    42781 ggcgcataaa agcgaaacgt caccggtcga tctgtgcgtg attggcattg tcgatgaggt
    42841 ggtgtctggc ggtcaggtaa ttttccacaa ataaggcaga acatcatgaa tcaacaggat
    42901 attgaacagg tggtgaaagc ggtactgctg aaaatgcaaa gcagtgacac gccgcccgcc
    42961 gccgttcatg agatgggcgt tttcgcgtcc ctggatgacg ccgttgcggc agccaaagtc
    43021 gcccagcaag ggttaaaaag cgtggcaatg cgccagttag ccattgctgc cattcgtgag
    43081 gcaggcgaaa aacacgccag agatttagcg gaacttgccg tcagtgaaac cggcatgggc
    43141 cgcgttgaag ataaatttgc caaaaacgtc gctcaggcgc gcggcacgcc aggcgttgag
    43201 tgtctctctc cgcaggtgct gaccggcgac aacggcctga ctttaattga aaacgcgcct
    43261 tggggcgtgg tggcttcggt aactccgtcc actaacccgg cggcaaccgt aattaataac
    43321 gccatcagcc tgattgccgc gggcaacagc gtcatttttg ccccgcatcc ggcggcgaaa
    43381 aaagtctccc agcgggcgat cacgctgctc aaccaggcga ttgttgccgc aggcgggccg
    43441 gaaaacttac tggttactgt ggcaaatccg gatatcgaaa ccgcgcagcg tttgttcaag
    43501 tttccgggca tcggccttct ggtggtgacc ggcggcgaag cggtagtaga agcggcgcgt
    43561 aaacacacca ataaacgtct gattgccgca ggcgctggca acccgccggt agtggtggat
    43621 gaaaccgccg acctcgcccg tgccgctcag tccatcgtca aaggcgcttc tttcgataac
    43681 aacatcattt gtgccgacga aaaggtactg attgttgttg atagcgtagc cgatgaactg
    43741 atgcgtctga tggaaggcca gcacgcggtg aaactgaccg cagaacaggc gcagcagctg
    43801 caaccggtgt tgctgaaaaa tatcgacgag cgcggaaaag gcaccgtcag ccgtgactgg
    43861 gttggtcgcg atgcggcgaa aatcgcggcg gcaatcggcc tgaacgtgcc gcaagaaacg
    43921 cgtttgctgt ttgtggaaac cactgcagaa catccgtttg ccgtgaccga actgatgatg
    43981 ccggtgctgc ccgtcgtgcg cgtcgccaac gtggcggatg ccattgcgct ggcggtgaaa
    44041 ctggaaggcg gttgccacca cacggcggca atgcactcgc gcaacatcga aaacatgaac
    44101 cagatggcga atgctattga taccagcatt ttcgttaaga acggaccgcg cattgccggg
    44161 ctggggctgg gcggggaagg ctggaccacc atgaccatca ccacgccaac cggtgaaggg
    44221 gtaactagcg tgcgtacgtt tgtccgtctg cgtcgctgtg tattagtcga tgcgtttcgc
    44281 attgtttaag gagtaacaga tggcgcacga cgaacaatgg ctcaccccac gtctgcaaac
    44341 ggcggcaacg ctgtgtaacc agacgcccgc cgcgacggaa tctccgctgt ggctgggggt
    44401 ggatctgggc acctgcgatg tggtgtcgat ggttgtcgac cgcgacggtc agtcggtggc
    44461 ggtatgcctc gactgggccg atgtggtccg cgacggcatc gtctgggatt tcttcggcgc
    44521 tgtcaccatt gttcgtcgcc atctcgacac gctcgagcag caattcggtc gccgttttag
    44581 ccatgcggcg acttcatttc ctccaggtac ggacccgcgt atttccatca acgtgctgga
    44641 gtctgctgga ctggaagtga gccatgtgct ggatgagcca accgcagttg cggatctgct
    44701 gcaactggac aacgccggtg tagtggatat cggcggcggc actaccggca ttgccatcgt
    44761 gaaaaagggc aaggtgacgt actcggcgga tgaagcaacc ggcggacatc acatctctct
    44821 gactctcgcc ggaaatcgcc gtatttcgct ggaagaggcg gagcagtaca agcgcggtca
    44881 cggtgaagag atttggccta cggtgaaacc ggtgtacgaa aaaatggcgg acattgtcgc
    44941 ccggcatatt gaagggcagg gcattactga tttatggctg gcgggcggct cttgtatgca
    45001 accgggcgtg gcggaactgt ttcgcaaaca attcccggcg ttacaggtgc atttaccgca
    45061 gcacagtttg tttatgaccc cgctggcgat cgccagtagt gggcgagaga aagcggaggg
    45121 gctatatgca aaatgaattg cagaccgcgc tctttcaggc gttcgatacc ctgaatctgc
    45181 aacgggtaaa aacatttagc gttccaccgg tgacgctttg cggtccgggc tcggtgagca
    45241 gttgcggaca gcaagcgcaa acgcgtgggc tgaaacatct gttcgtgatg gcagacagct
    45301 ttttgcatca ggcagggatg accgccgggc tgacgcgcag cctggctgtt aaaggcatcg
    45361 ccatgacgct ctggccatgt ccggtgggcg aaccgtgcat caccgacgtg tgtgcagccg
    45421 tggcgcagtt gcgtgagtca ggctgtgatg gggtgatcgc atttggcggc ggctcggtgc
    45481 tggatgcggc gaaagccgtg gcgttgctgg tgacgaaccc ggatagcacg ctggcagaga
    45541 tgtcagaaac cagcgttctg caaccgcgct tgccgctgat tgccattcca acgaccgccg
    45601 gaaccggctc tgaaaccacc aatgtaacgg tgattatcga cgcggtgagc gggcgcaagc
    45661 aggtgttagc ccatgcctcg ctgatgccgg atgtggcgat tctcgacgcc gcgttgaccg
    45721 aaggtgtgcc gtcgcatgtc acggcgatga ccggcattga tgcgttaacc catgccattg
    45781 aagcatacag cgccctgaac gctacaccgt ttaccgacag tctggcgatt ggtgccattg
    45841 cgatgattgg caaatcgctg ccgaaagcgg tgggctacgg tcacgacctt gccgcgcgcg
    45901 agagcatgtt gctggcttca tgtatggcgg gaatggcgtt ttccagtgcg ggtcttgggt
    45961 tgtgccacgc gatggcgcat cagccgggcg cggcgctgca tattccacac ggtctggcga
    46021 acgccatgtt gctgccaacg gtgatggaat ttaaccggat ggtttgtcgt gaacgcttta
    46081 gtcagattgg tcgggcactg cgaactaaaa aatccgacga tcgtgacgct attaacgcgg
    46141 taagtgagct gattgcggaa gttgggattg gtaaacgact gggcgatgtt ggtgcgacat
    46201 ctgcgcatta cggcgcatgg gcgcaggccg cgctggaaga tatttgtctg cgcagtaacc
    46261 cgcgtaccgc cagcctggaa cagattgtcg gactgtacgc ggcggcgcaa taaatgccgg
    46321 atgcgacgct tgccgcgtct tatccagcct acgggattgc acatgtaggg cggataaggc
    46381 gttcacgccg catccgccag tggcgcggtg caattgccgg atgcgatgct tgccgcgtct
    46441 tatccggcct acgggattgc acatgtaggg cggataaggc gttcacgccg catccgccag
    46501 tggcgcggtg caattgccgg atgcgacgct tgccgcgtct gatccggcct tcgggattgc
    46561 acatgtaggg cggataaggc gtttacgccg catccgccaa taaataacaa ctaacaggga
    46621 gtaaaggcga tgggaattaa cgaaatcatc atgtacatca tgatgttctt tatgctgata
    46681 gctgccgtag acaggatcct gtcgcagttc ggcggttctg ctcgtttcct cggtaagttc
    46741 ggtaaaagta tcgaaggatc aggcggtcag ttcgaagaag gctttatggc aatgggcgct
    46801 ctgggtctgg cgatggtcgg tatgaccgcg ctggcaccgg tactggctca cgtactcggg
    46861 ccggtaatta tcccggttta cgaaatgctc ggcgcaaacc cgtcaatgtt cgccggaacg
    46921 ctgctggcgt gcgatatggg cggcttcttc ctcgccaaag agctggcggg cggcgacgta
    46981 gcagcgtggc tatactctgg gttaattctt gggtcgatga tggggccaac gattgtgttt
    47041 tccattccgg tggcgctcgg cattatcgaa ccttctgacc gtcgttatct ggcgctcggc
    47101 gtgctggcgg gcattgtgac cattccgatt ggctgtattg ctggtggtct ggttgctatg
    47161 tactccggtg tgcagatcaa cggtcagccg gtggaattca ctttcgccct gatcctgatg
    47221 aacatgatcc cggtgatcat tgttgcgatt ctggtggcgc tggggctgaa attcatcccg
    47281 gaaaaaatga tcaacggctt ccagatcttc gccaaattcc tcgttgcatt gatcaccctc
    47341 ggtcttgctg ccgcggtagt taagtttctg cttggctggg aactgatccc cggtctggac
    47401 cctatcttta tggcccctgg cgataaaccc ggtgaagtga tgcgcgccat tgaagttatc
    47461 ggttctatct cctgcgttct gttaggggcg tatccaatgg tgctgctgct gacccgctgg
    47521 tttgaaaaac cgctgatgag cgtcggtaaa gtgctgaata tgaacaacat cgcggcagcc
    47581 ggcatggtgg caacgcttgc caacaacatc ccgatgttcg gcatgatgaa gcagatggat
    47641 acccgcggca aagtcatcaa ctgcgccttc gccgtttccg ctgctttcgc cctgggcgac
    47701 cacttaggct tcgccgctgc caacatgaac gccatgatct tcccgatgat tgtcggcaag
    47761 ttgatcggcg gcgtaacggc gattggcgtg gcgatgatgc tggtgccaaa agaagacgcg
    47821 accacggcta aaaccgaagc ggaggcacaa tcgtgaacac tcgccagcta ttgagcgtcg
    47881 gtatcgatat cggcaccacc accacccagg tgattttctc ccacctggag ctggttaacc
    47941 gtgcggcggt gtcgcaggtg ccgcgctacg aattcattaa acgcgaaatt agctggcaaa
    48001 gcccggtgtt ctttacccct gtcgataaac agggtggttt aaaagaagcg gaactgaaaa
    48061 ccttaatcct cgagcaatat caggctgcgg gcattgcgcc ggaaagcgtt gattctggtg
    48121 ccatcatcat caccggtgaa agcgcgaaaa cccgcaatgc tcgtccggcg gtgatggtgc
    48181 tctcccagtc gctgggcgat tttgtcgttg ccagcgccgg gccgcacctc gaatccgtca
    48241 ttgccggtca cggtgccggg gcacaaaccc tttctgaaca acggctgtgt cgggtactga
    48301 atattgacat cggcggtggc actgcgaact acgccctgtt cgatgccgga aaaatcagcg
    48361 gcactgcctg cctcaacgtc ggtggtcgcc tgctggaaac cgacagccag gggcgcgtgg
    48421 tttacgctca taaaccgggg cagatgattg tggatgagtg cttcggcgca ggcaccgacg
    48481 cccgttcgct gaccggcgcg cagctggtgc aggttacccg gcggatggca gaactgattg
    48541 tcgaagtgat tgacggaacg ctctcgccgc tcgcgcaggc attgatgcaa accggtttgc
    48601 tgcccgcagg tgttacgccc gaaatcatta cgctttctgg cggcgtgggc gaatgttatc
    48661 gccaccagcc cgccgacccg ttctgttttg ccgatattgg cccgctgctg gcaacggcgc
    48721 tgcatgacca tccgcgcctg cgtgagatga acgtacagtt tccggcgcaa accgtgcgcg
    48781 ccacggtgat tggcgcgggc gcgcataccc tttcgctctc tggcagcaca atctggcttg
    48841 agggcgtaca actgccgctg cgcaatttgc cggtggcgat cccgattgat gaaacggatc
    48901 tggtgagtgc ctggcaacag gcgctgcttc agctggatct tgatcccaaa actgacgcgt
    48961 acgtgctggc gcttcccgcc tcgctgcctg tgcgttacgc cgcggtactg acggtcatca
    49021 acgcgctggt cgatttcgtc gcgcgttttc caaatccgca tcccctgctg gtggtggccg
    49081 ggcaggactt tggtaaagct ctgggcatgt tgttgcgccc acagctacaa caactcccgt
    49141 tggcagtcat tgacgaagtg attgtccgcg cgggggacta tatcgacatt ggtacgcctc
    49201 tttttggcgg atcggttgtg ccggtgacgg tgaaatcact cgcatttcct tcctgaggga
    49261 acgacttatg aaactaaaga ccacattgtt cggcaatgta tatcagttta aggatgtaaa
    49321 agaggtgctg gctaaagcca acgaactgcg ttcgggggat gtgctggcgg gcgttgcagc
    49381 ggcaagctca caggagcgcg tggcggcaaa gcaggtgttg tcggaaatga ccgtagcgga
    49441 catccgcaat aatccggtga ttgcctatga agatgactgc gtgacgcggc tgattcagga
    49501 cgatgttaac gaaacggcct acaaccagat taaaaacagg agcatcagcg aactgcgtga
    49561 gtatgtgctg agcgatgaaa ccagcgtgga cgacattgcc tttacccgca aagggctgac
    49621 ctcggaagtg gtcgcggcgg tagcgaagat ttgctccaac gcggacctga tctacggcgc
    49681 gaagaaaatg ccggtaatca aaaaggccaa taccaccatc ggtattccgg gcaccttcag
    49741 cgcccgtttg cagccaaatg acacccgtga cgacgtgcaa agtatcgctg cacaaatcta
    49801 cgaagggctt tccttcgggg tgggcgatgc ggtgatcggc gttaacccgg taactgacga
    49861 cgtggaaaac ttaagccgcg tgttggacac catttacggc gtgatcgaca aattcaacat
    49921 cccaactcag ggctgcgtac tggcgcacgt caccacccag atcgaagcga ttcgtcgcgg
    49981 cgcgcctggc ggactgattt tccagagtat ctgtggcagc gaaaaagggc tgaaagagtt
    50041 tggcgtggaa ctggcgatgc tcgacgaagc gcgcgcagtg ggtgctgagt tcaaccgtat
    50101 cgccggggaa aactgcctct acttcgaaac cgggcaaggt tcggcgctgt ctgctggcgc
    50161 taacttcggc gcagaccagg tgacgatgga agcacgtaac tatgggctgg cgcgtcatta
    50221 cgatccgttt atcgtcaaca ccgtggtcgg ctttattggg ccggagtatc tctacaacga
    50281 ccgccagatt atccgtgctg gcttagaaga tcactttatg ggcaagctga gcggcatctc
    50341 tatgggctgt gactgctgtt acaccaacca cgctgacgct gaccagaacc tcaacgaaaa
    50401 cctgatgatc ctgctcgcca ccgcaggctg caactacatc atggggatgc cgctgggcga
    50461 tgacatcatg ctcaactatc agaccaccgc attccacgac actgccactg tgcgtcagtt
    50521 actcaacctg cgcccgtcac cggagtttga acgctggctg gaaagcatgg gcattatggc
    50581 aaacggtcgc ctgaccaaac gggcgggcga tccgtcactg ttcttctgat gacgcggaga
    50641 taacacatca tggatcaaaa acagattgaa gaaattgtac gcagcgtgat ggcgtcaatg
    50701 ggacaagcgg cccccgcgcc gtcagaagca aagtgcgcca ccactaactg tgcggcaccg
    50761 gtgacctcgg aaagctgcgc gctggattta ggttctgcgg aagcaaaagc gtggattggt
    50821 gttgaaaacc cgcatcgcgc agacgtatta acagaactgc gccgcagcac cgtggcccgc
    50881 gtttgtaccg gtcgtaccgg tccgcgtcca cgtacgcagg cgctgctgcg tttcctggcc
    50941 gatcactctc gttcgaaaga taccgtactg aaagaagtgc cggaagagtg ggtgaaagcg
    51001 cagggcttgc tggaagtacg ctccgaaatt agcgacaaaa atctctacct gactcgcccg
    51061 gatatgggcc gccgcctgtg tgcagaagct gttgaagcgc tgaaagcaca gtgtgttgct
    51121 aatccggacg tacaggtcgt tatttctgat ggcctgtcaa ctgatgccat caccgtgaac
    51181 tacgaagaga tcctgccgcc gctgatggcg ggcctgaaac aggccgggct gaaagtcggc
    51241 acgccattct ttgttcgtta tggtcgcgtg aagattgaag atcagatcgg tgagatcctc
    51301 ggcgcgaaag tggtgatcct gctggtgggg gaacgtccgg ggttgggtca gtcagaaagc
    51361 ctctcctgtt acgccgttta ctcgccgcgt atggcgacca ccgttgaggc cgatcgcacc
    51421 tgcatctcta acattcacca gggcggcacg ccgccggttg aagccgcagc cgtcattgtg
    51481 gatttggcga aacgtatgct ggagcagaaa gcatccggca tcaacatgac ccgataagga
    51541 ggcatcatgc cagctttaga tttgattcga ccgtcggtaa ccgccatgcg ggtgattgcc
    51601 tccgttaacg ctgaatttgc gcgtgaacta aaattgccgc cgcatattcg tagcctcggg
    51661 ctgatttctg ctgattccga tgacgtcacc tacattgcgg ctgacgaagc gaccaagcag
    51721 gcgatggtcg aagtggtata tggccgctcg ctgtatgcgg gagcagcaca cggaccgtca
    51781 ccgactgccg gtgaagtgct gattatgctt ggtgggccaa acccggcgga agtgcgtgct
    51841 ggtctggatg cgatggttgc gcatattgaa aatggcgctg ctttccagtg ggctaatgac
    51901 gcagaaaaca ccgcattcct ggcacatgta gtttcgcgta ccggttctta tctttcatca
    51961 accgccggga tcacgcttgg cgatccgatg gcgtatctgg tggcaccgcc gctggaagcg
    52021 acctacggca ttgatgcagc gttgaaatct gccgacgtac agctggtgac ctatgtcccg
    52081 ccaccgtctg aaaccaacta ctcggcagca tttttaaccg gtagccaggc cgcgtgtaaa
    52141 gcagcctgta acgcctttac cgatgcagtg ctggaaatcg cgcgtaatcc aatccagcgt
    52201 gcgtaacgga ggttgccgat gatcaatgca ctgggattgc tggaagtgga cggcatggtc
    52261 gccgcgatag atgcagcgga tgccatgctc aaagcagcta acgttcgtct gctcagtcac
    52321 gaagtgcttg accctggtcg cttaacgctg gtggtggaag gcgatctggc ggcgtgtcgt
    52381 gcggcgctgg acgcaggttg tgctgccgcg atgcgtaccg ggcgtgtcat cagccgcaag
    52441 gagatcggtc ggccagacga tgacacccag tggctggtta ctggctttaa ccgccagccg
    52501 aagcaacccg taaaggaacc cgacgcgcca gttatcgtcg cggaatctgc tgacgagttg
    52561 ttggcgctgt taacatcagt acgtcaggga atgacggcag gagaagtggc tgcccacttt
    52621 ggctggccgc tggaaaaagc cagaaatgcg ctcgaacagc tcttttctgc cgggacgtta
    52681 cgtaaacgca gtagtcgtta tcgtctcaag ccccattaac ctgtcggagg tgccgggtgt
    52741 cctacaacac ccggcattaa catcatgaaa aagacccgta cagccaattt gcaccatctt
    52801 tatcatgaac ccttacccga aaacctgaag ctcacgccga aggtcgaagt ggataatgtt
    52861 catcaacgac agacaacgga tgtctatgaa catgctttga cgattaccgc ctggcagcag
    52921 atttacgatc agctgcatcc gggcaagttt catggtgaat ttacggaaat tctgctcgat
    52981 gatattcagg tttttcgtga atacaccggt ctggcgctgc gtcagtcgtg cctggtctgg
    53041 ccgaactcgt tctggtttgg cattccggcg acgcgcggtg agcagggatt tatcggttcg
    53101 caatgtctgg gaagcgcaga aatagcgacg cgccccggtg gaaccgagtt tgaattaagt
    53161 acgccggacg attatacgat cctgggcgta gtgctgtctg aagatgtcat cacccgtcag
    53221 gctaactttt tgcacaatcc ggatcgggta ttacatatgc tgcgtagcca gtcggcgctg
    53281 gaagtgaaag agcagcataa agccgcgctg tggggctttg tccaacaggc gctggcgacg
    53341 ttttgcgaga acccggaaaa tctccatcag cctgcagtgc gaaaagtgct gggggataat
    53401 ttgctaatgg cgatgggggc gatgctggaa gacgcgcaac caatggtgac ggcggaaagc
    53461 atcagtcatc agagttaccg tagattactt tcccgcgccc gtgaatatgt gctggaaaat
    53521 atgtctgagc tggtgacggt gctggacttg tgtaatcaac tgcatgtcag tcgccgcacg
    53581 ctacaaaacg cgtttcacgc tattttaggc attggcccaa acgcgtggct gaaacgcatt
    53641 cgcctgaacg ccgtacgccg cgaactgata agcccgtggt cgcaaagcac aacggtaaaa
    53701 gacgccgcca tgcagtgggg attctggcat ctggggcaat ttgccacgga ttaccagcag
    53761 ctgtttgccg agaagccgtc gttgacgttg catcagcgga tgcgggaatg ggggtgaggg
    53821 agttacaccc aatccctgac cttaataaac tcacttaacg ccgcttctgg gctgccatct
    53881 tttggctgat aatcatactc ccagcgtacc agtggcggca ttgacatcag gatcgactcg
    53941 gtgcgcccgc cagtttgcag gccaaacaga gtgccgcgat cccagaccag attgaactcg
    54001 acataacgac cgcgacggta gagctgaaaa ttgcgctcgc gctcgccgta ggccatcgct
    54061 ttacgtcgct ctacaattgg taaataagcg tcggtgtagc ctttgcctac cgcctgcata
    54121 aaggcaaaac agtggtcgaa atctggcgtg ttcagatcat caaagaacag cccgccaata
    54181 ccgcgctgtt cgttgcgatg tttgaggtag aagtaatcgt cgcaccactt tttgtaacgg
    54241 ggataaacgt cttcaccaaa tggcaggcac aggtcacggg cggtgcggtg ccagtgaatg
    54301 gcgtcttctt caaaaccata gaaaggggtt aaatcgaagc cgccgccaaa ccaccagacg
    54361 ggctctgcac ccggtttttc ggcaataaaa aaacgcacat tcgcgtggct ggtgggaaca
    54421 tacgggttat gcggatgcac taccagtgaa acgcccatcg cctcgaaact gcgcccggca
    54481 agttccgggc gatgagcggt ggcggaagca ggcatcgcct cgccgtggac gtgagaaaag
    54541 ttgacgcctg cctgttcgaa aacaccacca ttacgcaaca cccgactacg cccgccgccg
    54601 ccagcttcgc gctgccaact atcttcgaca aattctgcgc catcgacggc ggtcagctgc
    54661 tgacaaatcg tatcctgaag gttgagcaga aactgtttaa cctggtgtgc gtcgggtttc
    54721 ataacttatc gctttttcga atgtgctttc tggttgtcga accagtggaa ataactgatc
    54781 acgccttcag caatcgctgt ggcgattttc tgacgaaacg ccgccgtgcc taacagccgc
    54841 tcttcttccg ggttggtgat aaacgaggtt tccaccagca ccgaaggaac cgacggcgat
    54901 ttcaacacca caaacgcggc ttgttcggtg ttgcggctgt gcagtttatg caccggctta
    54961 atctttttca gaatatgcga gccaagcgtc aggctgtttt taatagtatc ggtttgcacc
    55021 agatcaaaca gcacttgctg caataggtga tccttgtcag tcgccttttt accggcaact
    55081 tcatcggcgc ggttttcgcg ttcagacagg tatttcgcca ttgcgctact tgccccacgg
    55141 ttagagaggg caaataccga agcaccggca gctttcgggt tggtaaagcc atcggcatga
    55201 attgacataa acagatctgc gccatgttta tgggcgattt caacgcgatc gtaaagtggg
    55261 ataaacgtat cgccagaacg cgttaaacgc gcatcaatcc catgattacg caaaatggaa
    55321 cggacgtttt tagcaatcgc cagcaccaca tgtttttctt tcgaaccgtt gcgtccgatc
    55381 gctccggtat caattccgcc gtggcctgga tcgagaacaa cgacacgttt gccgccagat
    55441 tttttggctt tcggcttgct gtgtccgttg ctggttttta aaggttcgtc tttggcgatg
    55501 gcttgcgaca ttcctggcaa cgtcagggca gccaaaccgg ctttcagcac ctggcggcgc
    55561 gaagtgagtg tttttagtgg tttaaaagtg ctcatacggc ctgagttgta ataataaagt
    55621 tccagatgtt atatcgtatc gcgtaatccg ttacgacggt ttgatgttga gaattgtttt
    55681 acttttcatt tcaatagttg acaactcccc attatgccac tttttcaacc gattttcgtc
    55741 agatattggc taattcgacc gttcacgcca taatcagcct tttaacccgc aaaaaggtaa
    55801 cgaataccat ggagatacgc gtatttcgcc aggaagattt cgaagaggtc atcacccttt
    55861 gggagcgttg cgacttgctg cgtccgtgga acgatccgga aatggacatc gagcgtaaga
    55921 tgaaccatga cgtcagtttg tttttggtcg ctgaggtaaa cggtgaagtg gtcggaacgg
    55981 tgatgggcgg ttatgacggg catcgcgggt ctgcttatta tcttggcgtg catccagagt
    56041 ttcgtgggcg tgggattgcc aatgcgttgc ttaatcggct ggagaaaaag ctgattgctc
    56101 gtggctgccc gaaaattcag atcaatgtgc cggaagataa cgacatggtg ctcggaatgt
    56161 atgaacgcct gggatatgaa cacgccgacg tgctgagtct gggtaagcgt ttgattgaag
    56221 atgaagagta ctgagttcca tcctgtccat tatgatgcgc acggacgcct gcgtttaccc
    56281 ttgctcttct ggcttgtgct attgcttcag gcgcgaacct gggtgctgtt tgtcattgcc
    56341 ggtgcgtcgc gcgagcaggg tactgcgctg ctaaatctgt tttatcccga tcacgataat
    56401 ttctggctgg ggctgattcc aggcattccg gcggtgctgg cgtttttgtt gagcggtcgg
    56461 cgggctacat ttcctcgcac ttggcatgtg ctctattttc tgttgctgtt ggcgcaggtg
    56521 gttttacttt gctggcaacc gtggctgtgg ctgaacggtg aatccgttag cggtatcggt
    56581 ctggcgctgg tagtggcgga tattgtggcg ttaatctggc tgctgaccaa tcgacgttta
    56641 cgcgcttgtt ttaatgaaga aaaagaataa cggcactttt tggtgaattt gcactccaag
    56701 caacgttatt gaataaccaa aggaagtgac atgaaatcgc tgcgtttaat gttatgcgct
    56761 atgccgttga tgctgaccgg ctgttccacg atgtcgtcag ttaactggtc ggccgctaac
    56821 ccgtggaact ggtttggatc gtccaccaaa gtgagcgagc agggcgtggg tgaattaacg
    56881 gcgtccacac cactgcaaga acaagccatt gctgatgcgc ttgatggcga ttatcgcctg
    56941 cgcagcggaa tgaaaaccgc gaacggcaac gtggtgcgct tttttgaagt gatgaaaggc
    57001 gacaacgtgg cgatggtgat taacggcgat caaggcacga tcagtcgcat tgacgtgctg
    57061 gatagcgata ttcctgctga caccggtgtt aaaatcggta caccgtttag cgacctttac
    57121 agcaaagcat ttggcaattg ccaaaaagct gatggtgatg ataatcgtgc cgtcgaatgt
    57181 aaagccgaag gcagtcaaca tattagctac cagttcagcg gggaatggcg tggtcctgaa
    57241 gggttaatgc cttcggacga taccctgaaa aactggaaag tcagtaaaat tatctggcgg
    57301 cgttaatttg cgtctgaaca aaccgccgcc gcgaaaaaac acgtaaaata gcgctcaaca
    57361 atgccacgga ttgcgtggca ttcttatttt caggaggaac aatgtctcag gttcagagtg
    57421 gcattttgcc agaacattgc cgcgcggcga tttggatcga agccaacgtg aaaggggaag
    57481 ttgacgccct gcgtgcggcc agtaaaacat ttgccgacaa actggcaact tttgaagcga
    57541 aattcccgga cgcgcatctt ggtgcggtgg ttgcctttgg taacaacacc tggcgcgctc
    57601 tgagcggcgg cgttggggca gaagagctga aagattttcc gggctacggt aaaggccttg
    57661 cgccgaccac ccagttcgat gtgttgatcc acattctttc tctgcgtcac gacgtaaact
    57721 tctctgtcgc ccaggcggcg atggaagcct ttggtgactg cattgaagtg aaagaagaga
    57781 tccacggctt ccgttgggtg gaagagcgtg acctgagcgg ctttgttgac ggtacagaaa
    57841 acccggcggg tgaagagacg cgtcgcgaag tggcggttat caaagacggc gtggatgccg
    57901 gcggcagcta tgtgtttgtg cagcgttggg agcacaacct gaagcagctc aaccggatga
    57961 gcgttcacga tcaggagatg atgatcgggc gcaccaaaga ggccaacgaa gagattgacg
    58021 gcgacgaacg tccggaaacc tctcacctca cccgcgttga tctgaaagaa gatggcaaag
    58081 ggctgaagat tgttcgccag agcctgccgt acggcaccgc cagtggcact cacggtctgt
    58141 acttctgcgc ctactgcgcg cgtctgcata acattgagca gcaactgctg agcatgtttg
    58201 gcgataccga tggtaagcgt gatgcgatgt tgcgtttcac caaaccggta accggcggct
    58261 attacttcgc gccgtcgctg gacaagttaa tggcgttgta agcgtttctg attcgatgat
    58321 taaaggccag ccagtaacaa aactgactgg cctttttatt acttttgctt caacgccgca
    58381 tacacctgat caactaccca accataagtt gcaatcggta actgaccgct ttcgaacata
    58441 ttgggatttt tttgtggatc ggcaactggc gaatggggct tgttgcttaa catcacaatc
    58501 gccatatgat tgaccggatc gataacggtt actgtcccgg tccagccagt gtgaccgtag
    58561 gtttgcgggc ttgccagcgt gccaaacgtc ggcgtcatgg tggcattacc attcacgcgc
    58621 cagccgaggc caaaagtggc atcttcctta gagctggtgg tgaacatctt caccgtttcc
    58681 gcactgaaca gctgcacatc gccatagccc ccgccgttca gcatcgtttg cattaacacc
    58741 gcaatatcgc cggtattgga aaacaaacct gcgtgcccgg aaacgccgcc catcgaataa
    58801 aaggcttttt catcgtgcac ctgaccccag agagtggagg tgcggatatt cgggaaatgg
    58861 atcacgccat cgcgggtatt gccgttcaat tccgtggcgg caatttgttg cggtttaaag
    58921 cctttcagca gcgggttaaa taccgtatgt gtcaggccga gcgggcgata aatcgactct
    58981 tcaacatagc ggtcaagcgg ctgaccggta accgactcga cgataaatcc aagcagcata
    59041 taatcgacat cgctgtagat atgtttgctg ccgggctgat attccagcgg cgtgcgcttg
    59101 atcatctcca gcgtctggcc tttatcctgg gaatataacg cgcccgcgac cgctttattc
    59161 gggtattgcg gatctgccgg gaaaccgccg ctgtgatgca gcaggtcaga aatccgcagg
    59221 gtgtttttgc ctttaatggt gtcatttgga ctatcggcaa accccggaat atatttcgca
    59281 atccgatcgt caggatgcag tttgccttcg ctcatcagct tttgcagggc gaagttcgtg
    59341 gcgtacattt tggtgtttga ggccaggtca tacagcgtcc cggtggtggc tttgacaggc
    59401 tgttccatca acacgctgcc atcgtacttt ttcgccgctc cccaggcttt gcgatacaca
    59461 atctgattat ctttaatgat cagcaggttt acgccgggat aaccgacatc aatttgctgg
    59521 ctaatccagc gatccatctg gttaagccgt tcgacgttaa accctgcttt ctctggcgag
    59581 ctttctgtca gaacagggta ttttgcggcg ctgacgctac aactaacagc cagcagagaa
    59641 agatagagca ttgtccgttt catgatatca gtccagattg acgttacggc agccaaagag
    59701 cgtggtgaaa ataaacccgc aaacccatgc caccagaatc ccgccagcat aaaccgccat
    59761 ggccggaagg atgccttgtg cggaagtcat cagcggcagc gctaccagac cagacggccc
    59821 aaaggcgctg tttaagccca tcggcagacc ccatcaggct atcaggccga taaacaaacc
    59881 gcccgccgcg ccgcctaaac aggcggtaac aaacggcttc atgcggggca gggtgacacc
    59941 gtaaatcagc ggttcaccaa cgcccagcag gccgggaata atcgccccgc gtacctgact
    60001 gcgtagcgca ctgtgcggtt gcgcccgcca gtagagtgcc agcgccgcgc ccacctggcc
    60061 cgcgcctgcc attgaaagga tgggaaataa gctgttgaat ccctggctgt ccattaacgc
    60121 gaggtaaaca ggaataaagc cctgatgcac gccaaatacc acggcgatca ggaacagccc
    60181 ggctaaaacc gcacaaccga acggattact gttcaggtgc ataaacagcc atgacatacc
    60241 ttcgaataac cagccaccca gcggcataat gatcaggtac gcaagcgtag cggtgatcag
    60301 cagggtgatt aacgaggtca gcagcatgtc gagatcgtcc gacataaagc ggcgcaccat
    60361 gccttcaatg cgggcgcagg cccacgcggc aatcagcacg ccgataatat tgccgcgcgg
    60421 atcgatgggc agaccaaaga aatcgtgaaa accggcgtag taaccggtgg ttgcagcggg
    60481 gttataaccg agcaaaaaga gtgcggcaat aattgcgcca ttgacgcccg tgccgccaaa
    60541 tgcctgggcg gcgttatagc ccaccagaat caccaggaaa gtgaacaaac ctttgctgaa
    60601 caccttcata aaattcagcg cgtcgggtag cgtgccctga gcatctgccg gaacgtgcat
    60661 caccgtggca attaacgtcg ctatccccag cagcagaccg gcggcaataa aaccggggat
    60721 cagcggcgtg aagatggtgg cgaatttggc gagaaattgt tgcacgccgg aggtttgttt
    60781 ggcttttaac tgacgtttgt tctgggcggc gatttctgcg gcatcctgta ccggtgcctc
    60841 gcccagcagc tcgctcatcg cttttgcggc acgatgcgct ttaccaggcc caaaaacgac
    60901 ctgcacctgg tcgctggtca aaatgacgcc cttcacgcct tccagcgttt tgatattggg
    60961 atcaaccagt gaactgtcat gtacacccag acgcaggcgc gtcatacagt taccacaact
    61021 ggcgatattt cccagtccgc cgacacgggt aagaatggtg ttcagaagtt cactgctgat
    61081 ctctttggcc atgggttatt ccttgtctaa aacctgacga ataaagccgc cgtgttgatc
    61141 caggcgtttt ttagcttctg cggcatcgag atttttcagc accatcacaa tggccgtttt
    61201 acagttgcgc tcgcaggcaa ttaacgccgc ttccgcttgt tcggcattac atccggtggc
    61261 gtttttaacg atattgacct ggcgcacatg cagtttttcg ttggtggcga ccacatcgac
    61321 catcaggttg ccgaacactt tgccggattt aatcatcagc ccggtggaaa gcatattgag
    61381 caccagtttc tgcgctgtgc ctgctttcat tcgcgaagaa ccagtgacca cttcggcacc
    61441 tacaatcggt gtaatggcaa actcggcggt ggtggaaacg gcactccccg gattacagga
    61501 aattcccact gtgcggcaac cgagctggcg ggcgtattcc agtccggcaa tcacatacgg
    61561 cgtacgaccg ctggcggcaa taccgaccac cacatcctgt gccgttaaat ttatattttt
    61621 cagatcgtta atgccgcctt ctcggctatc ttccgtgcct tccaccgcgt gctgaatggc
    61681 atattcgccg ccagcaatca aaccaaccac cagacccggt ttcacgccgt aggtgggcgg
    61741 acattcgctg gcatccagaa tccccagacg accggatgtt cccgcaccga gataaatcag
    61801 acgaccgccg ccgcttacct gggcgtggat aacatcgatc gccgcggcga tatccggcag
    61861 gacgcgctca acggcaagcg gtacggtttt atcttcatcg ttgataatcc ggcacatttc
    61921 cagcgtcgat acgcggtcaa tttcagccga ggcggtgttc gagccttcag taatcatctt
    61981 ttcaagttgc atcggtggtg accttactat ttacgatcaa ataattgcag atgtatggaa
    62041 tcatatatcc cgaattgaca gacttaaagg aatcattgag tctacacttg cagagtgtga
    62101 agagcgtcat aagaacttaa gcggaatttt tgctgccagc ccgggagagt ggaagatgtt
    62161 gtacctgacg aaaatacgca atgcggaaag tgaatttacc gagaacgagc aaaaaattgc
    62221 tgactttctg cgggccaacg tgagtgagct gaaatccgtc tcttcgcgca agatggcgaa
    62281 acagctcggc atcagtcagt cgagcattgt gaaatttgcc cagaagctcg gcgcgcaagg
    62341 atttactgaa ttgcgtatgg cgttaattgg cgaatacagt gccagccggg agaaaacgaa
    62401 tgccacggcg ctgcacctgc acagttcgat taccagtgat gattcgctgg aagtgattgc
    62461 ccgcaaactg aatcgcgaaa aagagctggc gctggagcaa acctgtgcgt tgttcgacta
    62521 cgctcggctg caaaaaatca ttgaggtcat cagtaaagca ccgtttatcc agataacggg
    62581 cctgggcggg tcggcgctgg tggggcgcga tctgtcgttc aaactgatga aaattggcta
    62641 tcgcgtggcc tatgaagccg acacacacgt acaagccacc gtttcccagg cgttgaaaaa
    62701 aggcgatgta caaatcgcta tctcttacag cggcagtaaa aaagagattg tcttgtgcgc
    62761 agaagcggca cgaaagcagg gggcaacggt gattgccatt acttctctgg ctgactctcc
    62821 gctgcggcgg ctggcgcatt tcacgctcga taccgtttcc ggtgaaaccg aatggcgtag
    62881 ctcgtcgatg tctacccgca cggcgcaaaa ttctgttacc gatttactgt ttgttggtct
    62941 tgtgcaactc aatgatgtgg agtcattgaa aatgattcag cgcagcagtg agctaacaca
    63001 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
    63061 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
    63121 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
    63181 gcattgcagg gaattggcga aggaattgcc agaacttttg cgcgtcatgg cgcaaaccta
    63241 atcttgctgg atatctcccc tgagatcgaa aagctggcgg acgaactgtg tggtcgtggt
    63301 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
    63361 aaacgcgcga aggaaaaaga agggcgcatt gatatcctgg tgaataacgc aggcgtttgt
    63421 cgtctgggca gtttcctcga tatgagcgat gacgatcgcg atttccatat tgacatcaat
    63481 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc ccgcaaagat
    63541 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
    63601 gcgtacgcct taacgaaagc ggcgattgtt ggcctgacaa aatcgctggc ggtggagtac
    63661 gcgcagtctg gtattcgcgt taacgccatt tgcccgggat acgtgcgcac accaatggcg
    63721 gaaagcattg cccgccagtc gaacccggaa gatccagagt cggtgctgac tgaaatggcg
    63781 aaagcaatcc cgctgcgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    63841 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    63901 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatttg
    63961 tggggaggat ttcgttttaa acccggttct tattcccttt tcaacttcca aatcaccaaa
    64021 cggtatataa aaccgttact cctttcacgt ccgttataaa tatgatggct attagaaagt
    64081 cattaaattt ataagggtgc gcaatggccg ttaacttact gaaaaagaac tcactcgcgc
    64141 tggtcgcttc tctgctgctg gcgggccatg tacaggcaac ggaactgctg aacagttctt
    64201 atgacgtctc ccgcgagctg tttgccgccc tgaacccgcc gtttgagcaa caatggacca
    64261 aagaaaacgg cggcgacaaa ctgacaataa aacaatctca tgccgggtca tcaaaacagg
    64321 cgctggcaat tttgcagggc ttaaaagcag acgttgtcac ttataaccag gtgactgacg
    64381 tacaaatcct gcatgacaaa ggcaagctga tcccggcgga ctggcaaacg cgcctgccaa
    64441 ataatagctc gccattctac tccaccatgg gcttcctggt gcgtaagggc aacccgaaga
    64501 atatccacga ctggaacgac ctggtgcgct ccgatgtgaa gctgattttc ccgaacccga
    64561 aaacgtcggg taacgcgcgt tacacctatc tggcggcatg gggcgcagcg gataaagctg
    64621 acggtggcga taaagccaaa accgaacagt ttatgaccca gttcctgaaa aacgttgaag
    64681 tgttcgatac tggcggtcgt ggcgcgacca ccacttttgc cgagcgcggc ctgggcgatg
    64741 tgctgatcag cttcgagtcg gaagtgaaca acatccgtaa acagtatgaa gcgcagggtt
    64801 ttgaagtggt gattccgaaa accaacattc tggcggaatt cccggtggcg tgggttgata
    64861 aaaacgtgca ggccaacggt acggaaaaag ccgccaaagc ctaccttaac tggctctaca
    64921 gcccgcaggc gcaaaccatc atcaccgact attactaccg cgtgaataac ccggaagtca
    64981 tggataaact gaaagacaaa ttcccgcaga ccgagctgtt ccgcgtggaa gacaaatttg
    65041 gctcctggcc ggaagtgatg aaaacccact tcaccagcgg cggcgagtta gacaagctgt
    65101 tagcggcggg gcgtaattaa tgtttgcagt ttcatcaaga cgcgtgctgc cgggctttac
    65161 cttaagcctc ggcaccagtc tgctgtttgt gtgcctgatt ttgctgctgc cgctctccgc
    65221 gctggtgatg cagctggctc agatgagctg ggcgcagtac tgggaggtga tcaccaaccc
    65281 gcaggtagta gcggcctaca aagtaacgct gctgtcagcg tttgtggcat cgatttttaa
    65341 cggcgttttt ggcctgctga tggcgtggat cttaacccgc tatcgctttc cgggccgcac
    65401 gctgcttgat gcgctgatgg atttaccctt tgcgctgcca acggcagtcg ccggtttaac
    65461 gctagcctcg ctcttttctg taaacggttt ttacggtgaa tggctggcga agtttgatat
    65521 caaagtcacc tatacctggc tggggattgc ggtggcgatg gcctttacca gcattccgtt
    65581 tgtggtacgt accgtgcagc cggtgctgga agagttaggc ccggaatatg aagaagcggc
    65641 agaaacgctt ggcgcaacgc gctggcagag tttctgcaaa gtggtgctgc cggaactttc
    65701 tccggcgctg gtggcgggcg tggcgctgtc gtttacccgt agtcttggtg aatttggcgc
    65761 ggtgattttt atcgccggaa atatcgcgtg gaagacggaa gtgacgtcgc tgatgatttt
    65821 tgtgcgctta caggagtttg attacccggc agcgagcgcg attgcttcgg tgatcctcgc
    65881 ggcatctctg ctgctgctgt tctcaattaa cactctgcaa agtcgctttg gtcggcgtgt
    65941 ggtaggtcat taatggcgga agttacccaa ttgaagcgtt atgacgcgcg cccgattaac
    66001 tggggcaaat ggtttctgat tggcatcggg atgctggtat cggcgttcat cctgctggtg
    66061 ccgatgattt atatcttcgt acaggcgttc agcaaagggc tgatgccggt tttacagaat
    66121 ctggccgatc cggacatgct gcacgccatc tggctgacgg tgatgatcgc gctgattgcc
    66181 gtaccggtaa acctggtgtt cggcattctg ctggcctggc tggtgacgcg ctttaacttc
    66241 cctggacgcc agttactgct gacgctactg gacattccgt ttgccgtatc gccggtggtt
    66301 gccggtctgg tgtatctgct gttctacggc tctaacggcc cgctgggcgg ctggctggat
    66361 gaacataatc tgcaaattat gttctcctgg cccggaatgg tgctggtcac catctttgtg
    66421 acgtgtccgt ttgtggtgcg cgagctggtg ccggtgatgt taagccaggg cagccaggaa
    66481 gacgaagcgg cgattttgct tggcgcgtct ggctggcaga tgtttcgccg cgtcacattg
    66541 ccgaacattc gctgggcgct gctttatggc gtggtgttga ccaacgcccg cgcaattggc
    66601 gagtttggcg cggtgtcggt ggtttccggc tcgattcgcg gcgaaaccct gtcgctgccg
    66661 ttacagattg aattgctgga gcaggactac aacaccgtcg gctcctttac cgctgcggca
    66721 ctgttaacgc tgatggcgat tatcaccctg tttttaaaaa gtatgttgca gtggcgcctg
    66781 gagaatcagg aaaaacgcgc acagcaggag gaacatcatg agcattgaga ttgccaatat
    66841 taagaagtcg tttggtcgca cccaggtgct gaacgatatc tcactggata ttccttcagg
    66901 tcagatggtc gcgttgctgg ggccgtccgg ttccgggaaa accacgctgc tgcgcattat
    66961 cgccgggctg gagcatcaaa ccagcgggca tattcgcttc cacggcaccg acgtcagccg
    67021 cctgcacgcg cgcgatcgca aggtcggttt cgtgttccag cattacgcgc tgttccgcca
    67081 tatgacggta ttcgacaata tcgcttttgg cctgacggtg ctgccgcgtc gcgatcgccc
    67141 gaatgccgca gccatcaaag cgaaagtgac aaaattgctg gagatggtgc agcttgctca
    67201 tctggcggat cgttatccgg cacagctttc cggcggccag aaacagcgcg tggcgctggc
    67261 gcgcgcgctg gctgtagaac cgcaaattct gctgcttgat gaaccgtttg gcgcgctgga
    67321 tgcgcaggtg cgtaaagagc tgcgtcgctg gctgcgtcaa ctgcatgaag agctgaaatt
    67381 caccagcgtg ttcgtgaccc acgaccagga agaagcgacg gaagtagctg atcgtgtagt
    67441 ggtgatgagc cagggcaata ttgaacaggc tgatgcgccg gagcaggtat ggcgcgaacc
    67501 agcgacccgt tttgtgctcg aatttatggg cgaagtgaac cgcctgcagg gaaccattcg
    67561 cggcgggcag ttccatgttg gcgcacatcg ctggccgctg ggctacacac ctgcttatca
    67621 ggggccggtg gatctcttcc tgcgcccgtg ggaagtggat atcagccgcc gtaccagtct
    67681 cgattcgccg ctgccggtac aggtactgga agccagcccg aaaggtcact acacccaatt
    67741 agtggtgcag ccgctggggt ggtacaacga accgctgacg gtcgtgatgc atggcgacga
    67801 tgccccgcag cgtggcgagc gtttattcgt tggtctgcaa catgcgcggc tgtataacgg
    67861 cgacgagcgt atcgaaaccc gcgatgagga acttgctctc gcacaaagcg cctgataggt
    67921 tgagtgaatg ttaaacgccc ggaggcgctt cccgcgatcc gggcttttta atggcaaggt
    67981 ttgtaacctg tagacctgat aagacgcgca agcgtcgcat caggcaacac cacgtatgga
    68041 tagagatcgt gagtacatta gaacaaacaa taggcaatac gcctctggtg aagttgcagc
    68101 gaatggggcc ggataacggc agtgaagtgt ggttaaaact ggaaggcaat aacccggcag
    68161 gttcggtgaa agatcgtgcg gcactttcga tgatcgtcga ggcggaaaag cgcggggaaa
    68221 ttaaaccggg tgatgtctta atcgaagcca ccagtggtaa caccggcatt gcgctggcaa
    68281 tgattgccgc gctgaaaggc tatcgcatga aattgctgat gcccgacaac atgagccagg
    68341 aacgccgtgc ggcgatgcgt gcttatggtg cggaactgat tcttgtcacc aaagagcagg
    68401 gcatggaagg tgcgcgcgat ctggcgctgg agatggcgaa tcgtggcgaa ggaaagctgc
    68461 tcgatcagtt caataatccc gataaccctt acgcccatta caccactacc gggccggaaa
    68521 tctggcagca aaccggcggg cgcattaccc attttgtctc cagcatggga acaaccggca
    68581 ctatcaccgg cgtttcacgc tttatgcgcg aacaatccaa accagtaacg attgttggcc
    68641 tgcagccgga agaaggcagc agtattcccg gcattcgccg ctggcctgcg gaatatctgc
    68701 cggggatttt caacgcttct ctggtggatg aggtgctgga tattcatcag cgcgatgcag
    68761 aaaacaccat gcgcgaactg gcagtgcggg aaggaatatt ctgtggcgtc agctccggcg
    68821 gcgcggttgc cggagcactg cgggtggcaa aagctaaccc tggtgcggtg gtggtagcga
    68881 tcatctgcga tcgtggcgat cgctaccttt ccaccggggt gtttggggaa gagcatttta
    68941 gtcagggggc ggggatttaa gattattgcg caggcaccgt tttatccgtc tgcgcaatta
    69001 aggcatgtaa aaaaggctgt gcgttctcat cgctcttacc ggggatcttc acaatatacg
    69061 gcttcccggt aatcgatgac gacgaagcaa ccttatcaat aaactgctcg gcagtgtcaa
    69121 tgcggttacg ggtattgccg agcttcagac gcagatgaga aaccgcttca tcgcaggtat
    69181 gttcatcacc gttgcgcaca aatatcaaat cctttttctg tgctaatccc tccagcatgg
    69241 cgttgatgcg ggcttcttca tgaacagtta acttcgcgta agcggggagt gtcattagta
    69301 gtgtaatgac cagacaaatg attttcttca ttgaattacc ttatccttgt tttgtgatag
    69361 ccgatttgac tgccagaatc aggtaaagtt tcggttggaa tacgatgagg atcatcggca
    69421 aggagaaaaa attaaatgag tagtttgttg ttgtttaacg ataagagtag ggcgctgcag
    69481 gcggatatcg tcgccgtgca gtcgcaggta gtgtacggca gcgtaggcaa cagcattgcg
    69541 gtgcctgcta taaaacagaa cggcttgaat gtctttgccg tgccgacggt attgctgagc
    69601 aatacgccgc attatgacac tttctacggt ggtgcgattc cggacgaatg gtttagcggc
    69661 tatttgcgtg cgcttcagga gcgtgatgcg ctgcgccaac ttcgtgctgt aaccacgggc
    69721 tatatgggaa cggcatcgca aatcaaaatc cttgccgagt ggctgactgc gctacgcaaa
    69781 gaccatcctg acctattgat catggtcgat ccggtgattg gcgatattga tagcggaatt
    69841 tatgtcaaac ctgaccttcc cgaggcgtat cgacaatatt tactgccgct ggcgcaagga
    69901 attaccccca atatctttga gctggaaatc ctgaccggta aaaattgccg cgatctcgac
    69961 agtgccattg ctgccgcaaa aagtctgctt tcagacacat taaaatgggt ggtgattacc
    70021 agcgcctccg gtaatgaaga aaatcaggag atgcaggtgg tggtggtcag tgccgacagc
    70081 gtgaatgtca tttcccattc acgggtaaaa accgacctga aagggactgg cgacctgttt
    70141 tgtgctcagc ttatcagtgg tttgctgaaa gggaaggcgt taaacgatgc ggtgcaccgt
    70201 gcggggttgc gcgtactgga agtgatgcgc tacacccagc agcatgagag cgatgaattg
    70261 attttgccgc cgctggcgga agcataaaaa atggcgccga tg
//
LOCUS       DAZOZY010000002        66935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZOZY010000002 DAZOZY010000000
VERSION     DAZOZY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.002"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..581
                     /locus_tag="ACHURQ_000068"
                     /pseudo
     CDS             1..581
                     /locus_tag="ACHURQ_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            684..1186
                     /locus_tag="ACHURQ_000069"
                     /pseudo
     CDS             684..1186
                     /locus_tag="ACHURQ_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1253..2710)
                     /gene="nuoN"
                     /locus_tag="ACHURQ_000070"
     CDS             complement(1253..2710)
                     /gene="nuoN"
                     /locus_tag="ACHURQ_000070"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR0821958.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2717..4246)
                     /gene="nuoM"
                     /locus_tag="ACHURQ_000071"
     CDS             complement(2717..4246)
                     /gene="nuoM"
                     /locus_tag="ACHURQ_000071"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR0821959.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4410..6251)
                     /gene="nuoL"
                     /locus_tag="ACHURQ_000072"
     CDS             complement(4410..6251)
                     /gene="nuoL"
                     /locus_tag="ACHURQ_000072"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR0821960.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6248..6550)
                     /gene="nuoK"
                     /locus_tag="ACHURQ_000073"
     CDS             complement(6248..6550)
                     /gene="nuoK"
                     /locus_tag="ACHURQ_000073"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR0821961.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6547..7101)
                     /gene="nuoJ"
                     /locus_tag="ACHURQ_000074"
     CDS             complement(6547..7101)
                     /gene="nuoJ"
                     /locus_tag="ACHURQ_000074"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR0821962.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7113..7655)
                     /gene="nuoI"
                     /locus_tag="ACHURQ_000075"
     CDS             complement(7113..7655)
                     /gene="nuoI"
                     /locus_tag="ACHURQ_000075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR0821963.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7670..8647)
                     /gene="nuoH"
                     /locus_tag="ACHURQ_000076"
     CDS             complement(7670..8647)
                     /gene="nuoH"
                     /locus_tag="ACHURQ_000076"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR0821964.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8644..11370)
                     /gene="nuoG"
                     /locus_tag="ACHURQ_000077"
     CDS             complement(8644..11370)
                     /gene="nuoG"
                     /locus_tag="ACHURQ_000077"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR0821965.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11423..12760)
                     /gene="nuoF"
                     /locus_tag="ACHURQ_000078"
     CDS             complement(11423..12760)
                     /gene="nuoF"
                     /locus_tag="ACHURQ_000078"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR0821966.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12757..13257)
                     /gene="nuoE"
                     /locus_tag="ACHURQ_000079"
     CDS             complement(12757..13257)
                     /gene="nuoE"
                     /locus_tag="ACHURQ_000079"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR0821967.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13260..15062)
                     /gene="nuoC"
                     /locus_tag="ACHURQ_000080"
     CDS             complement(13260..15062)
                     /gene="nuoC"
                     /locus_tag="ACHURQ_000080"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR0821968.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15156..15818)
                     /gene="nuoB"
                     /locus_tag="ACHURQ_000081"
     CDS             complement(15156..15818)
                     /gene="nuoB"
                     /locus_tag="ACHURQ_000081"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR0821969.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15834..16277)
                     /gene="nuoA"
                     /locus_tag="ACHURQ_000082"
     CDS             complement(15834..16277)
                     /gene="nuoA"
                     /locus_tag="ACHURQ_000082"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR0821970.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16906..17844)
                     /gene="lrhA"
                     /locus_tag="ACHURQ_000083"
     CDS             complement(16906..17844)
                     /gene="lrhA"
                     /locus_tag="ACHURQ_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR0821971.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18764..19981
                     /gene="alaA"
                     /locus_tag="ACHURQ_000084"
     CDS             18764..19981
                     /gene="alaA"
                     /locus_tag="ACHURQ_000084"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR0821972.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20065..20664
                     /gene="yfbR"
                     /locus_tag="ACHURQ_000085"
     CDS             20065..20664
                     /gene="yfbR"
                     /locus_tag="ACHURQ_000085"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR0821973.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20723..22555)
                     /locus_tag="ACHURQ_000086"
     CDS             complement(20723..22555)
                     /locus_tag="ACHURQ_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR0821974.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22642..23290)
                     /gene="hxpA"
                     /locus_tag="ACHURQ_000087"
                     /pseudo
     CDS             complement(22642..23290)
                     /gene="hxpA"
                     /locus_tag="ACHURQ_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(23301..23795)
                     /locus_tag="ACHURQ_000088"
     CDS             complement(23301..23795)
                     /locus_tag="ACHURQ_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR0821975.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23878..24333)
                     /gene="yfbV"
                     /locus_tag="ACHURQ_000089"
     CDS             complement(23878..24333)
                     /gene="yfbV"
                     /locus_tag="ACHURQ_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR0821976.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24671..25873
                     /gene="ackA"
                     /locus_tag="ACHURQ_000090"
     CDS             24671..25873
                     /gene="ackA"
                     /locus_tag="ACHURQ_000090"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR0821977.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25948..28092
                     /gene="pta"
                     /locus_tag="ACHURQ_000091"
     CDS             25948..28092
                     /gene="pta"
                     /locus_tag="ACHURQ_000091"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR0821978.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28282..29802
                     /gene="yfcC"
                     /locus_tag="ACHURQ_000092"
     CDS             28282..29802
                     /gene="yfcC"
                     /locus_tag="ACHURQ_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR0821979.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29835..30377)
                     /gene="yfcD"
                     /locus_tag="ACHURQ_000093"
     CDS             complement(29835..30377)
                     /gene="yfcD"
                     /locus_tag="ACHURQ_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR0821980.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30435..30986)
                     /gene="yfcE"
                     /locus_tag="ACHURQ_000094"
     CDS             complement(30435..30986)
                     /gene="yfcE"
                     /locus_tag="ACHURQ_000094"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR0821981.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31042..31686)
                     /gene="yfcF"
                     /locus_tag="ACHURQ_000095"
     CDS             complement(31042..31686)
                     /gene="yfcF"
                     /locus_tag="ACHURQ_000095"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR0821982.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31822..32469
                     /gene="yfcG"
                     /locus_tag="ACHURQ_000096"
     CDS             31822..32469
                     /gene="yfcG"
                     /locus_tag="ACHURQ_000096"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR0821983.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32526..32888
                     /gene="folX"
                     /locus_tag="ACHURQ_000097"
     CDS             32526..32888
                     /gene="folX"
                     /locus_tag="ACHURQ_000097"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR0821984.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32909..33802
                     /locus_tag="ACHURQ_000098"
     CDS             32909..33802
                     /locus_tag="ACHURQ_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR0821985.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33850..34740)
                     /gene="rpnB"
                     /locus_tag="ACHURQ_000099"
     CDS             complement(33850..34740)
                     /gene="rpnB"
                     /locus_tag="ACHURQ_000099"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR0821986.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34937..35710)
                     /gene="hisP"
                     /locus_tag="ACHURQ_000100"
     CDS             complement(34937..35710)
                     /gene="hisP"
                     /locus_tag="ACHURQ_000100"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR0821987.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35718..36434)
                     /locus_tag="ACHURQ_000101"
     CDS             complement(35718..36434)
                     /locus_tag="ACHURQ_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0821988.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36431..37117)
                     /gene="hisQ"
                     /locus_tag="ACHURQ_000102"
     CDS             complement(36431..37117)
                     /gene="hisQ"
                     /locus_tag="ACHURQ_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR0821989.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37207..37989)
                     /gene="hisJ"
                     /locus_tag="ACHURQ_000103"
     CDS             complement(37207..37989)
                     /gene="hisJ"
                     /locus_tag="ACHURQ_000103"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR0821990.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38210..38992)
                     /gene="argT"
                     /locus_tag="ACHURQ_000104"
     CDS             complement(38210..38992)
                     /gene="argT"
                     /locus_tag="ACHURQ_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR0821991.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39258..39827)
                     /gene="ubiX"
                     /locus_tag="ACHURQ_000105"
     CDS             complement(39258..39827)
                     /gene="ubiX"
                     /locus_tag="ACHURQ_000105"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR0821992.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39922..41439)
                     /gene="purF"
                     /locus_tag="ACHURQ_000106"
     CDS             complement(39922..41439)
                     /gene="purF"
                     /locus_tag="ACHURQ_000106"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR0821993.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41476..41964)
                     /gene="cvpA"
                     /locus_tag="ACHURQ_000107"
     CDS             complement(41476..41964)
                     /gene="cvpA"
                     /locus_tag="ACHURQ_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR0821994.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42223..42885)
                     /gene="dedD"
                     /locus_tag="ACHURQ_000108"
     CDS             complement(42223..42885)
                     /gene="dedD"
                     /locus_tag="ACHURQ_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR0821995.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42875..44143)
                     /gene="folC"
                     /locus_tag="ACHURQ_000109"
     CDS             complement(42875..44143)
                     /gene="folC"
                     /locus_tag="ACHURQ_000109"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR0821996.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44213..45127)
                     /gene="accD"
                     /locus_tag="ACHURQ_000110"
     CDS             complement(44213..45127)
                     /gene="accD"
                     /locus_tag="ACHURQ_000110"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR0821997.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45283..45942)
                     /locus_tag="ACHURQ_000111"
     CDS             complement(45283..45942)
                     /locus_tag="ACHURQ_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0821998.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46025..46837)
                     /gene="truA"
                     /locus_tag="ACHURQ_000112"
     CDS             complement(46025..46837)
                     /gene="truA"
                     /locus_tag="ACHURQ_000112"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR0821999.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46837..47850)
                     /locus_tag="ACHURQ_000113"
     CDS             complement(46837..47850)
                     /locus_tag="ACHURQ_000113"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0822000.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47916..49052)
                     /gene="pdxB"
                     /locus_tag="ACHURQ_000114"
     CDS             complement(47916..49052)
                     /gene="pdxB"
                     /locus_tag="ACHURQ_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR0822001.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49151..50146
                     /gene="flk"
                     /locus_tag="ACHURQ_000115"
     CDS             49151..50146
                     /gene="flk"
                     /locus_tag="ACHURQ_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR0822002.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50143..51321)
                     /locus_tag="ACHURQ_000116"
     CDS             complement(50143..51321)
                     /locus_tag="ACHURQ_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0822003.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51596..52816)
                     /gene="fabB"
                     /locus_tag="ACHURQ_000117"
     CDS             complement(51596..52816)
                     /gene="fabB"
                     /locus_tag="ACHURQ_000117"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR0822004.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            52975..54981
                     /gene="mnmC"
                     /locus_tag="ACHURQ_000118"
     CDS             52975..54981
                     /gene="mnmC"
                     /locus_tag="ACHURQ_000118"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR0822005.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55102..55380)
                     /locus_tag="ACHURQ_000119"
     CDS             complement(55102..55380)
                     /locus_tag="ACHURQ_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR0822006.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55414..55962)
                     /gene="epmC"
                     /locus_tag="ACHURQ_000120"
     CDS             complement(55414..55962)
                     /gene="epmC"
                     /locus_tag="ACHURQ_000120"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR0822007.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55962..56771)
                     /locus_tag="ACHURQ_000121"
     CDS             complement(55962..56771)
                     /locus_tag="ACHURQ_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR0822008.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56771..57595)
                     /gene="mepA"
                     /locus_tag="ACHURQ_000122"
     CDS             complement(56771..57595)
                     /gene="mepA"
                     /locus_tag="ACHURQ_000122"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR0822009.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57599..58684)
                     /gene="aroC"
                     /locus_tag="ACHURQ_000123"
     CDS             complement(57599..58684)
                     /gene="aroC"
                     /locus_tag="ACHURQ_000123"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR0822010.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58719..59651)
                     /gene="prmB"
                     /locus_tag="ACHURQ_000124"
     CDS             complement(58719..59651)
                     /gene="prmB"
                     /locus_tag="ACHURQ_000124"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0822011.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            59817..60368
                     /gene="smrB"
                     /locus_tag="ACHURQ_000125"
     CDS             59817..60368
                     /gene="smrB"
                     /locus_tag="ACHURQ_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR0822012.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60490..61362)
                     /locus_tag="ACHURQ_000126"
     CDS             complement(60490..61362)
                     /locus_tag="ACHURQ_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR0822013.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(61349..61873)
                     /locus_tag="ACHURQ_000127"
     CDS             complement(61349..61873)
                     /locus_tag="ACHURQ_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822014.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61870..62340)
                     /locus_tag="ACHURQ_000128"
     CDS             complement(61870..62340)
                     /locus_tag="ACHURQ_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822015.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(62337..62885)
                     /locus_tag="ACHURQ_000129"
     CDS             complement(62337..62885)
                     /locus_tag="ACHURQ_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822016.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62860..63611)
                     /locus_tag="ACHURQ_000130"
                     /pseudo
     CDS             complement(62860..63611)
                     /locus_tag="ACHURQ_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63631..66273)
                     /gene="yfcU"
                     /locus_tag="ACHURQ_000131"
                     /pseudo
     CDS             complement(63631..66273)
                     /gene="yfcU"
                     /locus_tag="ACHURQ_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66355..66918)
                     /locus_tag="ACHURQ_000132"
     CDS             complement(66355..66918)
                     /locus_tag="ACHURQ_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822017.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16647 a        18164 c        16693 g        15431 t
ORIGIN      
        1 ttatttttac caaccctggg tcactgcatt atcgccaacg ccccgacacg tcagtcctga
       61 gcaattagaa aaaacagttc gttatcttac acaaactgtg catccacgta atgccgacaa
      121 tattgataat ctgaataggt ccgtagagta tataaaagag ttctttgtca gtagcggtgc
      181 cagagttacc gcgcagagcg tccccgattg gctattacga ctctgcgcct ggtagccagg
      241 attaccctta cccgcaatgt cctggcttta tcccgatcgg ggggacttta ttgcctggtc
      301 ggcagaatac aggatatcaa cgccgttcgt caggtaaaaa cggcattgtt atcatcccgg
      361 gatttatctg tttattctat gaatgcccca tggtttattc ccggtattga tttctctgac
      421 cacctgaatt attggcaaca cgatattccc gccgtaatga ttactgacac cgctttttat
      481 cgtaataaac aataccactt gcccggtgat acagcagaca gattgaatta tcagaaaatg
      541 gctcagatgg tgttataacc ttgttacaca acagtaaata ataaaaattt ctcgaggcgg
      601 gtccagaaac agcgacatgt acattttatt ttccctcgtc tgttttaaga tgcgaaacat
      661 aaaattcaat ggaggaaaaa taaatgggaa tgattggtta ttttgcagaa attgactcag
      721 aaaagattaa ccaactgctt gagtctactg aaaaaccttt gatggataat atacatgaca
      781 cgctttcagg attacgccga ctggatattg ataaacgctg ggatttttta cactttgggc
      841 tgacaggcac ccctgctttt gatcccgcga agaatgatcc gctgagtcgt gccgtgcttg
      901 gtgaacacag cctcgaagat ggcatagatg gcttcctggg attgacctgg aatcaggagt
      961 tagccgcgac aatcgaccga ctggaatcac tggatcgaag tgagctgcgt aaacagtttt
     1021 ccattaagcg actcaatgaa atggagattt atccgggagt gaccttttca gaagagttag
     1081 aggggcaact ctttgcttcg atcatgctga tatggaaaag ctgattagcg cgtatcgacg
     1141 tatgctgcga caagggaatc atgcgctgac ggttattgtt gggtgatggt gtcagggcac
     1201 caaaaaaaac cgccgaacat gtcggcggct ttctgactta caaactgata gcttacatca
     1261 gcggcattgc taaccgcaca atgctaatca gcggttgtgg ccatacaccc agcaccagta
     1321 ccaacagtgc agagatcagc accacgatac cgcctgcgct gtactgccag tttgacggtg
     1381 catcgcgacc cggttgttcc ggggcgtgca ggtacaggct caccgccacg cgcaggtagt
     1441 agtaaaggcc gcttgccgaa ccgacaacca cggcgcccac cagccaccac aagtgtgcct
     1501 ggacaccgac cgccagcacg tagaacttac caataaagcc cagcgtcatc ggaataccgg
     1561 ccagagacag catcatcacc gtcatcaccg ccgcgaggat cgggcgatgc cagaacagtc
     1621 cgcggtaaga gtacagggaa tcagcatccg ggccacgata cgggctggac atcaggctga
     1681 ccacgccgaa cgcgccgagg ctgctaaaca gataaccggc caggtaaacc cctaccgctt
     1741 ccatcgacat ctcgccagtt tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag
     1801 agatagatga gtaaccgagc agacgtttga tattggtctg gctcagcgcc atcaggttac
     1861 cgaagatgat ggaggcaaag gcgataatcg ccagcaccac gcgaatcgct tcgctgtcac
     1921 ccatcggtgc gtagaggaac agacgcatca ccacaccgaa gatagcgatt ttgctcgccg
     1981 tcgccaggaa agtggaaacc ggcgcaggcg cgccctggta tacgtctggc gtccacaggt
     2041 ggaacggcac cagagagagt ttgaagccga ggccaacaat catcaggccg aaacctgcca
     2101 gcaacagcgg ctcgttgagc ataccgtcgc caaggttttt acccaacgcg acaaacgaca
     2161 ggtcgccaga ctgcgcatac accagcgcca taccaaacag caggaaagaa gacgctgcgg
     2221 cagaaaggat ggtgtatttg atactggctt ccagtgaacg tttctggcgg aaagcgtaac
     2281 cgaccaggcc aaacagcggc aaagagatca gttcgatacc gaggaacaga gacgccagat
     2341 ggttggcatt cgccagcagg atcccgccca gcgcggcaat taacaccaac aggtagaact
     2401 cgtccttgtt gtcgttatag ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc
     2461 tcgccaacaa taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg
     2521 taacgtccat agcgcctgcc tggccgacaa accagagcga aaccagcgcc gcgttaagcc
     2581 caataaccga gagcgtagcg ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca
     2641 caaccaccac cgtcaagccg acgatcagca acggtagcag tgcgatcagg ttttgtggag
     2701 ttattgtcat ggcgatttac ggccttgtag tagtaacgga attaacaaac cactgctgga
     2761 tattgccaat cgcggagtgc gaggtatcca gaatcggctg cggatagaag ccctgcagta
     2821 ccagcagcac caccagcaac aggatcataa acagctcacg cagcgacatc cctggcagtt
     2881 cctggctggc aatctcgctt ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg
     2941 aataaacaga tgcaaagacc agcccaaagg tagagataac ggtaatcacc gggacaacct
     3001 ggaagctgcc gaacagaatc ataaattcgc cgacgaagtt accggtgcca ggcatcccaa
     3061 gcgttgccac cgcaaagaac agcgacagtg ctggcagcca tttcatcttg ctccacagac
     3121 cgcccatcat gcgcatgtcg cgggtatgga tacgttcata aagctgacca caaagaataa
     3181 acagacccgc cgccgacaag ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca
     3241 actggctgcc ggtgtagata gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg
     3301 cgatcagacg tttgatatcg gtctgggcga aggccatcca cgcaccgtag aagatgccga
     3361 taacacccag ccacatagca attggcgcga actctgccga cgcgttcggg aacagcggca
     3421 gggagaaacg cagcaaaccg taagcggcag ttttcagcaa gatccccgcg aggtcaacgg
     3481 aaccggcggt cggagcctgg gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg
     3541 gcattttgac tgcgaaggcg atgaagaagc ccagcattaa caggtattcc gcaccactgg
     3601 acattggcgt attcagcagc tcttcatagt tgaaggtcca gacgccggtc gcattgtagt
     3661 gaacaaaaac cagcgccagg atggcaatca acatcaccag accactcgcc tgggtgtaaa
     3721 tgaagaactt ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta tgcccccaca
     3781 gtgcgatcag gaagtacatc ggcaccagca tcatttccca gaagaagaag aacaggaaca
     3841 tgtcgatggc aaggaacacg ccgataacgc cgcccaggat ccacatcagg ttgaggtgga
     3901 agaagccctg atatttttcg atctctttcc acgaacatag taccgccagc acaccgagca
     3961 gaccggtcag cacgaccatc agcagcgaca gcccgtcaat ggcgagatga atagagatac
     4021 ccaaacgcgg gatccacggc atgtcgaatt cagactgcca ctgcggaatt ccggcggatt
     4081 gcgtcagtga ataaccgccc tgcaaccaca gttgcagcga cagcgccagc gtcaatccca
     4141 tggtgatcag cgcgatccag cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca
     4201 ggaagccgcc aataaaggga attaatatta gccagggtag taacatggcg atctttattc
     4261 cttgtaaaag tcccgtcagg accggatttt caacgaattc tcacgacaga aaattctctt
     4321 cggactgggg gtagtgtcgg atgcaccgcg aaggcggctt atccgaccta cgttcttata
     4381 tgcccagggg caatcccaca atccttaact caacgcagta ccatcaacag tgccagcacc
     4441 acgaccgcac cgatgctcat ggatgccaca taccagcgca gatagccgtt ctcacttaac
     4501 agcagacctt tacctgcaaa gcgggaaagg acagccggaa tgttcatcat tgagttcagc
     4561 ggatcgcgtt tcagcaacca ggcaataccc aggaacggcc tgacgaacac tttgtcgtac
     4621 agccagtcaa agccccaggc gttgtaccac caggtactca gcaggcggcc cggcgcactg
     4681 ttggcgatgg aggtcaccag agtacgttta cccagccaca gccaagctgc cagcagaatg
     4741 ccgacaatcg cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagt
     4801 tccgtcgttt gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg
     4861 gaaaggatca gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg
     4921 tgaatttgtt cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc
     4981 ataaacgcac cgaccagacc tgccaccatc agattgatat gaccattcgc catcgcaccc
     5041 gcgaggatct catccttact gaagaagccc gcagtgacca gcggcagtgc cgacagtgct
     5101 gcgccgccca ccaggaagca gagataaacc agcggaatag atttacgcag accgcccatc
     5161 ttgaagatgt tctgttcgtg atggcaggcc agaatgacgg aaccggatgc caggaacagc
     5221 agcgctttaa agaacgcgtg agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg
     5281 ccaagcgcga ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg
     5341 atgtcggtct gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccgacaatg
     5401 cccaccagat gcagaatttc cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg
     5461 tagacacccg cggttaccat ggttgcggcg tggatcagcg cggagacagg cgtcgggccc
     5521 gccatcgcgt cggcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg
     5581 cccagcagca tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt
     5641 gccagttcca ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt
     5701 gcgaaagcga ggaaaacgtc acccacacgg gtcacgacga acgctttcat tgccgctgcg
     5761 ccattcttcg gatcggtgta atagaaaccg atcagcagat aggagcacag gcccacgcct
     5821 tcccagccga ggtacatcag cagcaggttg tcggcaagca ccagaactac catgctggcg
     5881 atgaacaggt tggtgtaggc gaagaagcga gagtagccct cttcaccgcg catgtaccag
     5941 gaggcgtaca tgtgaataag gaaacccaca ccagtgacca ccgagagcat ggtcagcgac
     6001 aggccgtcca gcaccaggtt aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac
     6061 agcggctggc tgtatgcctg ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt
     6121 accagcgccg ccaggcccac agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag
     6181 cgcccacggg agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca
     6241 agcatgttca tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt
     6301 gaagttgcag cagcagcgca aggccgatac tcgcttctgc cgccgcgagg ctgatggcga
     6361 gaatgtacat cacctgaccg tcggtctgac cccagtagct tccggcgaca acaaaggcca
     6421 gcgcggaggc gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga
     6481 taaccagacc ggttaagcca agaatgaata agattgccgc gaggatcagt ccatgttgta
     6541 aggggatcat gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag
     6601 cacttcaccc gcacgctctt cacgaccgac gtggaaggcc acaaccagac ctgcgagcag
     6661 cagcatagaa gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc
     6721 tttagcactg attggcgtac cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac
     6781 aatcaccacc agcatgatgg ccgacaaaat tgccggacca atccacacct gcggtttcag
     6841 ccactggcgt tcctgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac
     6901 gaacagcacc ataatggcac ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc
     6961 gcccagtgag aagaacaccc cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc
     7021 gtgtaccgga ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca
     7081 gatataaaaa gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata
     7141 ggcttggctt cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc
     7201 cggtagaagt tatattccgg gtatttgccc ggaccggaga tcagcagatc ctctttttcg
     7261 taaaccagat cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc
     7321 gtggtcggac aggcttcttc gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg
     7381 aaaaattccg gataccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg
     7441 accgggcagg ctaccgcgca gaggttacag gctacgcaac gctcttcgcc gtccgggtcg
     7501 cgggtcagaa cgatacgacc acgataacgg ggcggcagat agatcggctc ttccgggtac
     7561 attcgcgttt cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg
     7621 gtgccgaaac ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct
     7681 gccagagaat gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt
     7741 tccagccgaa ggacattacc tggtcataac gcggacgcgg taacgacgca cgaatcaaaa
     7801 tgaacatcat cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg
     7861 ggccttgcca gccaccgaag aacagcgtca ccatcaatgc agagatggtc acaatcccga
     7921 tgtactcacc cacgaagaac agaccgaact tcataccgga atattcaatg tggtaaccat
     7981 ccgccagttc ctgctcggct tccggctggt caaacgggtg acggtgacat accgccacgc
     8041 ccgcgatggc aaaggtaata aaaccaaaga attgcgggat aacgttccac acatgcgcct
     8101 ggctgttgac gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaggg
     8161 aaagcccgag gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcacccagca
     8221 acgagtattt gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagacctg
     8281 ccatcatcag gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga
     8341 ctggcacaat cgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa
     8401 agatgacgcg atccgagaat ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt
     8461 ccgcaaccag ctggagcgaa ccgccccagc caacacggtt aggtccgtaa cggttctgga
     8521 acagacccag caggcgacgt tcgccaaagc tcatgaatgc cccgcaggtg acaaccacca
     8581 gcaggatcac caccgctttg aggatggtca gcaggatctc aatcagttcc ggtgatatcc
     8641 aactcattgt tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat
     8701 accggacata cccatcggca agcccacctg ccctgccgtc agcccttcag cgatttcaac
     8761 cggcagcgtg accgtattgc catcgtaact aaaggagacg tgtgtacctg cgttcacacc
     8821 taacttcgcg gcatccgctg ggttgagttt gatgtacggc tgcggcatac ggctctggaa
     8881 gaccggagca cgctgtgaca attcatcgct gccaaacagg tggtaatacg gcgcgatacg
     8941 ccatttcccg tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt
     9001 ttcgctggtt tcaaacagac gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc
     9061 gtcctggaat ttgttccacg cctgcgggga gttccagccc ggcgcccagg caaacggcac
     9121 ttgcgaacgg tgcgcagtcg gctggttgtt accttccatc gagaaggtga acatggtgtc
     9181 aatatcctgc ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc
     9241 gctgtaacgg tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatccgg
     9301 cgcagcatct ttgatacctg ccagttccgg gattttcgcc acaacagcgt caatcacatg
     9361 gtcgagctgc gtccagtcca cttcacggct cagcagggtg ctgtgcagcg agtgtaacca
     9421 gcgccagctt tccagcatga cagttttgct gtcgtaataa gcaggatcgt aaacctggaa
     9481 gaaacgttgg gcgcggcctt cgttgttgat caccgtaccg tcgctttcag caaaactggc
     9541 agcagaaagt accagatggg cgttttccat aatcgctgtg cgttgatgat caaccaccat
     9601 caccagcggc gctttagcca gcgcagcatt cacgcgggta gcggaagcgt gacgatgcag
     9661 atcgttttcc agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg tcaacgcttc
     9721 ttcaagcgaa ccgccaccca taatgcccag ccccatgctg ttgacggaac gggcaatcat
     9781 ggtgatacca acgtcagcgc cgcgaccttt cagggctttc gcgacgttag ccgcagcctg
     9841 aatcacctct atgctaccgg cgttcgtccc ggagataatc aacggtttct tcgcacctgc
     9901 cagtgcctgc acgatgacgt cgattttgct ttgcagctca ggttcgatac cgtcaaccgc
     9961 tggcgcagag ttatccagcg catgggcgat ggcaaaacct aaacgcgcct gatcttcaac
    10021 cggtgcgcgg taagtccacg ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac
    10081 aaacagcgga tgcttcgcac gttgaccgat gttgaggatt gccgcaatct gccagtcagc
    10141 cactttctgt gctgccgcca tttcgcgcgc tttacctttc acagcctgac gcactgccag
    10201 cgcgacgcgc gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga
    10261 ttcgatttcg cgcagagccg gagtataaat gccgccttca cgcagcactt tcagcgccag
    10321 ttgcagacgt tcctgctcac cgtgagcgat accggtgtag aagttttctt cgcccaccag
    10381 ttcacgcagc gcaaagttgc tttccacgct ggcacgcgga gaaccaatac cgatcacttt
    10441 cttcgactga cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat
    10501 gaaatcatcg ccacgacgct gtactggctg acgcggacga tccttcaggt tgacgtaacc
    10561 gtaaccgaaa cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt
    10621 ttcgatacga cgcagttcgc cgtaacgttc accggggctg atgttacagc cgatggaaca
    10681 ttgctggcag atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg
    10741 cgttttgtcg gtaaatacgc cggtcgggca aatttcgacc aggttaccgg aaaattcgct
    10801 ttccagcgtg ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaacgcc
    10861 cagatctgta ccgtcagcgt aatctttgta gtaacgcaca cagcggtagc aggcgatgca
    10921 gcggttcatt tcgtgagaga tgaatggccc caaatcctga ttacggtggg tacgtttggt
    10981 gaaacggtag cgacggaagc tgtgtccggt catcacagtc atatcctgaa gatggcagtt
    11041 accgccttct tcacataccg gacagtcgtg cgggtggttg gtcattaacc actcgaccac
    11101 actttcacgg aactgtttcg cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc
    11161 cggtgtcata caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt
    11221 caccgcacac tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg
    11281 aatatcaagg cccagagaca gacaagcttc cagcaggttg tccgctccgt tgacctcgta
    11341 ttctttgccg tctacatgaa ttgtagccat tagcatgctt ccagttttct cggtcaggga
    11401 ccgagcgtta atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat
    11461 taatcaaatg ggtattgctg aacggctgtt tgattcccgc ctcaaattct tcgcggaaat
    11521 atttgatggc gctctgtaac ggctccactg caccaggtgc gtgggcacag aaagttttac
    11581 ccgggcctaa gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tggccttcgc
    11641 cacgctccag cgcacgcaga attttcacgc tccacggcag accgtcgcgg cacggcgtac
    11701 accagccgca ggactcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt
    11761 tgatctcatg gtcaaccgcc atcgccagcg ccgtacccag acggctgccc gctttaccga
    11821 tactttcgaa ttccatcggc agatcaaggt gcgcttcggt caggaagtca gtccccgcgc
    11881 cgcctggctg ccaggcttta aatttcagac catcacgcat accaccggcg taatcttcga
    11941 ggatctcgcg tgcggtggtg ccgaacggca gttcccacag tcccggattt ttcacccgac
    12001 cggagaagcc catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact
    12061 ccacgccgtt agcgaggatc gccggaacgt tacacagggt ttcgacgttg ttgacacagg
    12121 tcggtttgcc ccatacgccg gaggttgccg ggaagggggg tttcgagcgt gggttagcac
    12181 gacgcccttc cagggagttg attaacgctg tttcttcccc gcagatgtag cgccctgccc
    12241 cggtatggac gaacagttcg aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc
    12301 ccgcttcagt ggcttcggca atggcgcggc gcagatttac tgccgcttcg atatattcgc
    12361 cacgcaggaa gatgtagcca cggtaagctt tcagcgcaaa cgcggagatg agcatacctt
    12421 ccaccagcag gtgcggcagt tgctccatca acaggcggtc tttataggtg cccggctcca
    12481 tttcatcggc gttacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc
    12541 tccacttcag gccagtcgaa aagcccgctc cgccgcgccc tttcagacca gcgtctttta
    12601 cctgattaac gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac
    12661 cgtttttgct gcggtattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg
    12721 tcagcggatg cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc
    12781 agggatcgct tccggggtca gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc
    12841 tttatcacag ttccccaggc agcaagttgg cagcagcgta aagcggccat caaatgtcgt
    12901 ttgccctggt ttgatgttca gctttttctc gagcgccgcc tgaataccct gatacccgtt
    12961 gatatgacag accacgctgt cgcaataacg gatcacatgg cgaccaaccg gctggcggaa
    13021 gatctgactg tagaacgttg ccacaccttc gacgtcgctt gccggaatac ccagcacatc
    13081 ggcgatcgcg tggatcgcac catccggcac ccagccacgc tgcttctgaa cgattttcag
    13141 cgcttcaatg gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc
    13201 acgctctgcc gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat
    13261 tagcggtcca catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac
    13321 accaggctgc cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg
    13381 atacgagtgc gataactcat ggtgctaccg tcgctggtca ggtagtaact gttgatcccc
    13441 ttggtcgcct caatcatctg gaaagattca ttggcaggca tcaccggccc ccacgacact
    13501 tgcaggaagt gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc
    13561 gtggtcagcg ggtgatccgc tttgaacggg ccttccggca tgttgttgag gcactgctca
    13621 agaatgcgca gactctggcg cagctcttcc actttcagca ttacgcgggt gtagcagtca
    13681 gaaacaccac caccaaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc
    13741 gccttacgca cgtcgaagtc gatcccggta gcacgcaggc ccgcgccagt ggtgccccac
    13801 tccagcgcct ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggta
    13861 ttttgcagcg ccgctttctc gtaagacgcc agacgtttcg gcatccagtc gaggaactca
    13921 cgcagcaggc gatcccagcc gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac
    13981 gccgggtgca tacggaaacc cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg
    14041 gtaaaggcga agaacacggg cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac
    14101 agcaggtggc tgttgatacg gaacagttcg gagagcataa cgcgaatgac gttaacgcga
    14161 tccggcacgg tgatcccggc cagtttctct accgccagca cgtaaggcat ttcgttaacg
    14221 cagccgccga ggtattcaat acggtcggta tacggaatgt agctgtgcca ggactggcgc
    14281 tcgcccattt tctccgcacc acggtggtgg tagccgatgt ctggtacgca gtcgacaatc
    14341 tcttcgccgt cgagttgtaa aacgatacgg aaagccccgt gcgccgacgg gtggttcgga
    14401 ccgaggttga ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc
    14461 ggtttgaagg tcagggcttc catctccaga tcctgtttgg ctttggtcag ctcaaacggc
    14521 gagaattcgg tagcgcgcgc cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc
    14581 ggcatcatga tgcggcgcag gttcgggtga ccgtcgaaag taatgccaaa cagatcccag
    14641 gtttcacgct cataccagtt agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg
    14701 tcgttttctg ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga
    14761 tggtagaaaa cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg
    14821 tccatgccgt gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg
    14881 ccaacttcca gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc
    14941 tgaacagtaa aggcatccgg cccaaaacgg ttgcgcagtt cgccaatcac cggatcatca
    15001 agatgatcac gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc
    15061 attgcaaatg gtccgtggtg actgtcggcg cagtaagctt cgcgaaatag atttaggaat
    15121 aagcgaagaa aaatcccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg
    15181 ttggttacgg caatgcgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga
    15241 taaacgccct gatcgccaac cacccaggag agcggacgac gttctttgcc gatagattcc
    15301 tgcaacagca tcagtgcctg catgtacgct tcaggacgcg gcgggcagcc cgggatatac
    15361 acatcaaccg ggatgaattt atcgacgccc tgcacaacgg aataaatatc gtacatacca
    15421 ccagagttgg cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac
    15481 agacgctgaa taaccggtgc cattttggta aagcaggttc ctgcaaccac catcaggtca
    15541 gcctgacgcg gcgaagcacg caatacttct gcgccaaaac gcgccacgtc atgcaccgcg
    15601 gtaaacgaag tcaccatctc aacgtaacag caggaaagac cgaagttata cggccaaatt
    15661 gagtttttac gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttgtta
    15721 acttcttgct ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc
    15781 tcaccgttgg gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt
    15841 tgacgattag cgatactgtt cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc
    15901 gtccagtcca gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata
    15961 aaaattgcag cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg
    16021 aacagataca gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacttg
    16081 gcggacaggc gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggcacgttt
    16141 ttcgacctcg cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacac
    16201 aggccaatgg caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg
    16261 gatgttgaca tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg
    16321 gcagatggac gccacatcga ttcatgggga ggaataaaaa aaaccttaca atcactgtag
    16381 aaattctttt atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa
    16441 ctttaacaaa agtttcttca cattagttta cataatatca acaccattag catttaatgc
    16501 cctttcacgc cagatccttg acgactccag gataattaga tgttgttgaa tcgtgtccgt
    16561 tgtgaagcaa tggaaaaaat acgggtctat tttgacagga atttgtgtcg attcctcccc
    16621 ccaaaagaga gtattttctt gatctgtgac acgcttttgt cattccataa caaaaacgca
    16681 gcaacaaatt tacgtatttt taacatcatt gtagcaggtg atttttttca ggcgattatt
    16741 tgtgcgttcg ggacgtgaat ctctggtgat tgaaaaatga acagttttgt acgttctgca
    16801 ctatgcgagg aaggattttt actaaaaaaa agccgctggg gtttaaaaca cccccagcgg
    16861 ctcgttttta cactattgtc tcaggaatta tctatcgtcc gtcgattact cgatatccct
    16921 ttcaatcaac aatgaatcat ccccttccgg agcagacatt gggctgtatt gccacggatt
    16981 atggtagctc tccatcgcct gatagatcac ctgggccagc tcattattac tggacgggtc
    17041 atagcacagc aaatattcgg tatcaggtag cggcggtaag ccatctactc cactcagcac
    17101 acgcaggtcc gggctcatca tttcaatcgg ccttgccgtc acgccaagac cggctttcac
    17161 tgccgcacga acggccggaa gcgtcgaggc gacataagcc agtcgccatg gaatatctgc
    17221 tttattaagc gtcgccagca ccatatcgcg aaacggactt ggatcatcca gcaatacaag
    17281 agggatcggc tcgccttttt gcaatacgta ttccgctgcg cagtaccagt gtgttggcga
    17341 ggtacgcagg ttcaatgcct taaacgctga aggacgatgg gtggttacca tcaaatccac
    17401 ttcctgggat tccagcattt ctgccatata ggcattacgt ttaacgcgga catccagcgc
    17461 taatttcgga taaaccgaac tcacgcgatt taacaggaaa ggtaagatcg tatcggcaga
    17521 ttcatctgaa gcaccgatag ttaacacgcc ctgaagatta ctgaacatta atgatgagca
    17581 ggcctcatca ttaaaacgca gtattttcct ggcgtaacca agaagttgaa tgccatgttc
    17641 agttaacagt ttgttgcgac cgtgacgagc gaacagttct ttcccaacga gttgttccag
    17701 acgctgcatt tgctgactta cggcggactg agtacgacac acagcggcag ctgcggcagc
    17761 aaaagtgttc agatcggcaa cagcaacaaa tgttctcagc agatcgaggt cgaggttaat
    17821 tatcggacga tttgcactta tcatatatta tcacttactg gcggctcata ctgagctggt
    17881 taatgctgtg cacacacaaa caagcaattc catttgtaat gtgcctccct ggcagtttca
    17941 tccggaaccc ggacgaaagt aaaaatgcat atgagttgca ctaaaaaagc gactcacatt
    18001 gttccgttat aatgcctgaa gtagatcaca gaatatatct tcagggatcg catatctatt
    18061 aagttactca ctcttttcta tttatgacat gcgcgtgttt gtataaatgt aaatgtgagt
    18121 ccttgttcca ctctcgtgca gcatcgctgg tcatacgcga acacgtacaa acagcaatgg
    18181 tgaggcggca tcaagagcag ggatccgtca tttatgcgag cattttaccc caaaaacctt
    18241 ttatttataa gggtcattgc gaattatctg atgcaaagtt atgttatgtt aggcaaagta
    18301 atcttctatt attaataagc acatcaaaac ctttttgaat attaaataat aattaattag
    18361 catcatcctc attcattaat tccgcttaac aatagtttca caccttgccc ccattgcgac
    18421 acctcggaaa tcatcacgct gttaaactct ccgtaatgcg gaaatatcat catcgcgttt
    18481 cattagtgaa ttcttaaatg aggcattttc acacaattat cttacagata aaaaaaccag
    18541 acttgcaatt aagaatcaga acaattcact atataacatt gcatgcaaag catatacacc
    18601 tcattatttt gtcattatta agttattaac agcacaatcg agccttcccc tctggcaaaa
    18661 tcttattctg cagaccttca aaacaccgtc ctgggggagt acattgttct aagctgactt
    18721 ccacggcagg gagtggcgat aacagcaaaa aaggtcaaga ttcatgtccc ccattgaaaa
    18781 atccagcaaa ttagagaatg tctgttatga catccgtggt ccggtgctga aagaagcaaa
    18841 acgcctggaa gaagaaggta acaaggtact gaaactgaac atcggcaacc cagccccatt
    18901 cggttttgac gcgccagatg aaatcctcgt tgacgtgata cgcaacctgc ctaccgctca
    18961 agggtattgc gattccaaag ggctttactc cgcgcgtaaa gccatcatgc agcactacca
    19021 ggctcgtggc atgcgtgatg ttaccgtgga agatatttac atcggcaatg gtgtatcgga
    19081 gcttatcgtt caggcaatgc aggcattgct gaacagcggg gacgaaatgt tggttcctgc
    19141 accagattac ccactctgga ccgcggcggt ttcgctttcc agcggtaaag cggtgcatta
    19201 tctttgcgat gaatcctctg actggttccc ggacctcgat gatattcgcg ctaaaattac
    19261 gcctcgtacg cgtgggatcg ttattatcaa cccaaataac ccaaccggcg cggtatattc
    19321 caaagagctt ttaatggaga ttgtggagat tgcacgtcag cataatctca ttatcttcgc
    19381 cgatgaaatt tatgacaaaa ttctctacga cgatgctgag catcactcaa ttgcgccact
    19441 ggcacctgac ctgctgacca ttacctttaa cggactgtcg aaaacgtacc gcgttgcagg
    19501 cttccgtcag gggtggatgg tgttgaacgg gccgaaaaaa cacgccaaag gctacatcga
    19561 aggtctggaa atgctggctt caatgcgcct gtgtgctaac gttcctgcgc aacacgccat
    19621 tcagaccgcg ctgggtggtt atcagagcat cagtgaattt attacccctg gcggtcgtct
    19681 ttatgagcag cgtaaccgcg cgtgggaact gatcaacgat attccgggcg tttcctgcgt
    19741 gaaacctcgt ggtgcgctgt atatgttccc gaaaatcgac gccaaacgct ttaacattca
    19801 cgacgatcag aaaatggtgc tggatttcct gttgcaggaa aaagttctgt tggtgcaagg
    19861 gacggcattc aactggccgt ggccggatca cttccgcatt gtcacgctac cgcgtgtcga
    19921 tgatatcgag ctgtctttga gcaagttcgc gcgtttcctt tctggttatc atcagctgta
    19981 atcttaattt cactgccgga gattgcatcc ggcagcgtta tcccgccaca atgacctgat
    20041 gatgtcatca tacgtaaggt cactatgaaa cagagccatt tttttgccca tctctcccgc
    20101 ctgaaactca ttaaccgctg gccgctaatg cgcaacgtgc ggacggaaaa tgtgtccgaa
    20161 cacagtttgc aggtagcgat ggtcgcccat gcgctggcag ctatcaaaaa tcgaaaattt
    20221 ggcggtaatg tcaacgccga acgtatcgct ttactggcga tgtaccacga tgcctcagaa
    20281 gtgctcaccg gcgatctccc tactccggtg aaatacttca attcgcaaat cgctcaggag
    20341 tacaaggcta ttgaaaaaat cgctcagcaa aaactggtcg atatggttcc ggaagagctg
    20401 cgggatatct ttgcgccgtt aattgacgag catgcatata gcgatgaaga aaaatcgctg
    20461 gtgaaacagg cagatgcact gtgtgcatat ctgaaatgtc tggaagaact cgcggccgga
    20521 aataatgaat tcttgctggc aaaaacgcga ctggaagcga cgcttgaagc gcgtcgcagc
    20581 caggagatgg actacttcat ggaagtattt gttcccagct tccatctttc gctcgatgag
    20641 attagccagg attcaccgct gtaagcagcc ggagtctgcg tcgcatcagg caataagcgc
    20701 cggatgcgac atcaggctct tgtcaaaacg gaaacagcat cgggatcatc accacacaaa
    20761 ccgccatcac gataatggtg aacggtaccc ccaacttcac aaagtcactg aagctgtaat
    20821 ttcccggacc taaaaccagt gtgttaacag gtgaagaaac cggtgtcata aaggcggcgg
    20881 atgctgccat cgccacgacc atcgcgaatg gataaggcga cacccccatc gttttggcag
    20941 cagccagcgc aatcggagcc atcaacaccg ccgtcgcggt attagagata aatagcccaa
    21001 taaccgccga caagacaaac aaacacccca gcatcatatg tggcccgtaa ccaccgccaa
    21061 tgtccatcag ccctttcacc gccagcgcga cacctcccgt tttctgtaat gccacagcaa
    21121 acggcatcat cccaacgatc aaaataatgc tcggccagtg aatggattta taggcgcttt
    21181 cagcatctat acagcggaat ttccccatca gcaggcaggc gatgatagcg gcaacaggat
    21241 taggaatttc atctgtcagc attaacgcca ccattagcac cagacagaaa atggcatggg
    21301 gtgcctggct gtgcgcgggc gatgcttcac tcacctcttc cggtaagttc agcgctacga
    21361 agtcgcggcc ctgtttggcc agcataccga tcagtttcca gttacccaca accaggatga
    21421 tatcgcccag cagcagaggc tcatccgcca gcgaaccttc cagcgccacg ccattgcgct
    21481 ttagccccac cacattcagt ccgtagcggg tacgaaaacc aatttcgcgc accgatttac
    21541 caatcagttc tgactcagga attaatgaaa tctctgccat gcccacatca agggcctggt
    21601 cagaaaaata ctcgccgcgc agtaccatcg gctccagcaa ttgctcacta caaaattgcc
    21661 ggagatcgac atcagccgca gacatatcaa taagcaaaac gtcacgcgcg cgaaattcag
    21721 aaaccccatt aacgttcacg ataacgcgac gaaaacgccg ccagcgttca acaccgatga
    21781 cgttagcgcc ataacgctca cgtaatttga gatcatccag ccgttgacca atcattggcg
    21841 atccggggcg aatagccaga cgtcgcgcac gcccggtcag tcgatattca cggataagat
    21901 cgcgaaaggt tcgacgcgtc cagccttcgc gctgcggggt ctgggtatcc cctttcagca
    21961 tgaaacgcat cactaacata tacaaaatac ccagcaccag cacgaccagg ccaataggtg
    22021 ttacgctaaa gaaactgaag ccgtgatagc cttcacgcag caattcactg ttgactacca
    22081 ggttcggcgg cgtcgccacc agcgtcatca tgccgcttat cagcccggca aaactcaacg
    22141 gcatcatcag acgtgacggc gaagtttgca tacgcatggc aacgcttaac accacgggaa
    22201 taaagatagc gacaacgccg gttgaactca taaacgcgcc aagccccgcg acggtcagca
    22261 tcaacaaaac caacattttg atttcactgt tgcccgcaac tttgaccagc catgttccca
    22321 ttacggtggc aacaccggta cggaccaaac catcgccaat aataaacaag gcggcaatca
    22381 ggacaacgtt aggatcagaa aagccggaaa atacttcggg gactgtcagc gttccgctta
    22441 atgcaaacgc gacaataaca aacaaagcga ccgcatccat acgcactctg cccgtcgcaa
    22501 acaagacaat ggcaacggcc agtaatgaaa gaacccaaat caattcaccg ttcacaactt
    22561 atccttgtta attgagggag atgtcttgat tctgccataa aaaagccccg acgagacggg
    22621 gctaaatcat gatcaggtgt ttcactgaat aataacatcg ccatttggct gtttggtcac
    22681 agtaatttgc tccagactgt ggaggaccaa atcgacctca ttcaggcgcg gggtatctgc
    22741 cggagcgtta accgcaatga catgacaacc cgccgccagg ccagaaagca cgccagcgga
    22801 gcatcttcca ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc gcctaacaga
    22861 tacgcatcag gttctggttt tccgcgcttc actcgctcag cggttacaaa cacctctggt
    22921 gcgggaagcc cagctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca
    22981 atggcccacg gaatacctgc tttattcaaa tgactgagta aggcgattgc ccccggaagc
    23041 gggtaatacc ttcggtttcc gtggcctcga tgtgctccag acgcgtaaac tcggcggcaa
    23101 tatcagcctc ggatttgccc gccataaaat ggcgcagaga ggtgatcgcc tgtttaccgt
    23161 gaatgaaagc cagcacctct tccggcgcta acccatgacg tctggcccag ttgctccacg
    23221 cccgttctac cgcaggcagg gaatccacca gcgttccatc aagatcaaac agaaaacctt
    23281 tgcaccgcac gcgggcctcc tcaggcattg ataatttgat taatttcgtt ggcgctcaaa
    23341 tggtactgac gcgggcaggc atgccacaca ttaagcatgc gctgatattt ttcccacatt
    23401 ggcgtctggg cgttaaaacc gtgagttccg gcgtcaaaat gggtatagcg cccttccaca
    23461 ttaaccataa agcggacata accgaggtaa cgtgcttcag tggcggcgtc aaagccgagg
    23521 aaggtgacac gacgttcatc gatggattgc tggtcctgca aattagacca ggaaacatgc
    23581 aacgcatgat acatctccat aatgtcgatg atagtgcggc aggtttcttc tttcagctcg
    23641 ccaaactcgc gatccaattc acgcatctgt aatccgtaac ctcgctcaat aattgtttgc
    23701 aggcgacggt aacgttcagc atttgccgga tcgagcatag tcatcatctt gtactgatta
    23761 gacaaaataa gacgttgcgc gttggtcatt tccattgttg actcctgtat cactctacta
    23821 cggtgaaaaa aaagaaggct gagtatgcct tcttttatat gcgtaatcag gggtcaatta
    23881 caaatcatca aggaaagttt tatccagttg tttgaaggcg cgcttaagcg tgtcagctaa
    23941 tgcctggtaa tcaggcttgc cttcaacggg tgccaacacc tgtccagact cctgcaattt
    24001 accgcgaact tcataaaacc agttaaggat tgcagggggt aatggcgtta cagaacgctt
    24061 gcccagccac cacaatccct gcatgggtaa acttaaggcg aacagtgcag tggcaactgc
    24121 cggcccaagc tgaccgccca gggcaatctg ccagcagaga gtaaatacgg cgatcggcgg
    24181 cataaaacgg atcgcataac gcgtcatctt gataacgcga ttttcgacaa agaccggggc
    24241 aaggcgtttt tccagtggcc acgtctttga gtaatgctgt ccgcggcgaa acaagctaaa
    24301 aaaattaaca gaacgattat ccggcgttga catgcttcac ctcaacttca catataaaga
    24361 ttcaaaaatt tgtgcaaatt cacaactcag cgggacaacg ttcaaaacat tttgtcttcc
    24421 atacccacta tcaggtatcc tttagcagcc tgaaggccta agtagtacat attcattgag
    24481 tcgtcaaatt catatacatt atgccattgg ctgaaaatta cgcaaaatgg catagactca
    24541 agatatttct tccatcatgc aaaaaaaatt tgcagtgcat gatgttaatc ataaatgtcg
    24601 gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt tttagccacg tatcaattat
    24661 aggtacttcc atgtcgagta agttagtact ggttctgaac tgcggtagtt cttcactgaa
    24721 atttgccatc atcgatgcag taaatggtga agagtacctt tctggtttag ccgaatgttt
    24781 ccacctgcct gaagcacgta tcaaatggaa aatggacggc aataaacagg aagcggcttt
    24841 aggtgcaggc gccgctcaca gcgaagcgct caactttatc gttaatacta ttctggcaca
    24901 aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac cgtatcgtac acggcggcga
    24961 aaagtatacc agctccgtag tgatcgatga gtctgttatt cagggtatca aagatgcagc
    25021 ttcttttgca ccgctgcaca acccggctca cctgatcggt atcgaagaag ctctgaaatc
    25081 tttcccacag ctgaaagaca aaaacgttgc tgtatttgac accgcgttcc accagactat
    25141 gccggaagag tcttacctct acgccctgcc ttacaacctg tacaaagagc acggcatccg
    25201 tcgttacggc gcgcacggca ccagccactt ctatgtaacc caggaagcgg caaaaatgct
    25261 gaacaaaccg gtagaagaac tgaacatcat cacctgccac ctgggcaacg gtggttccgt
    25321 ttctgctatc cgcaacggta aatgcgttga cacctctatg ggcctgaccc cgctggaagg
    25381 tctggtcatg ggtacccgtt ctggtgatat cgatccggcg atcatcttcc acctgcacga
    25441 caccctgggc atgagcgttg acgcaatcaa caaactgctg accaaagagt ctggcctgct
    25501 gggtctgacc gaagtgacca gcgactgccg ctatgttgaa gacaactacg cgacgaaaga
    25561 agacgcgaag cgcgcaatgg acgtttactg ccaccgcctg gcgaaataca tcggtgccta
    25621 cactgcgctg atggatggtc gtctggacgc tgttgtattc actggtggta tcggtgaaaa
    25681 tgccgcgatg gttcgtgaac tgtctctggg caaactgggc gtgctgggct ttgaagttga
    25741 tcatgaacgc aacctggctg cacgtttcgg caaatctggt ttcatcaaca aagaaggtac
    25801 ccgtcctgcg gtggttatcc caaccaacga agaactggtt atcgcgcaag acgcgagccg
    25861 cctgactgcc tgatttcaca ccgccagctc agctggcggt gctgttttgt aacccgccaa
    25921 atcggcggta acgaaagagg ataaaccgtg tcccgtatta ttatgctgat ccctaccgga
    25981 accagcgtcg gtctgaccag cgtcagcctt ggcgtgatcc gtgcaatgga acgcaaaggc
    26041 gttcgtctga gcgttttcaa acctatcgct cagccgcgta ccggtggcga tgcgcccgat
    26101 cagactacga ctatcgtgcg tgcgaactct tccaccacga cggccgctga accgctgaaa
    26161 atgagctacg ttgaaggtct gctttccagc aatcagaaag atgtgctgat ggaagagatc
    26221 gtcgcaaact accacgctaa caccaaagac gctgaagtcg ttctggttga aggtctggtc
    26281 ccgacacgta agcaccagtt tgcccagtct ctgaactacg aaatcgctaa aacgctgaat
    26341 gcggaaatcg tcttcgttat gtctcagggc actgacaccc cggaacagct gaaagagcgt
    26401 atcgaactga cccgcaacag cttcggcggt gccaaaaaca ccaacatcac cggcgttatc
    26461 gttaacaaac tgaacgcacc ggttgatgaa cagggtcgta ctcgcccgga tctgtccgag
    26521 attttcgacg actcttccaa agctaaagta aacaatgttg atccggcgaa gctgcaagaa
    26581 tccagccctc tgccggttct cggcgctgtg ccgtggagct ttgacctgat cgcgactcgt
    26641 gcgatcgata tggctcgcca cctgaatgcg accatcatca acgaaggcga catcaatact
    26701 cgccgcgtta aatccgtcac tttctgcgca cgcagcattc cgcacatgct ggagcacttc
    26761 cgtgccggtt ctctgctggt gacttccgca gaccgtcctg acgtgctggt ggccgcttgc
    26821 ctggcagcca tgaacggcgt agaaatcggt gccctgctgc tgactggcgg ctacgaaatg
    26881 gacgcgcgca tttctaaact gtgcgaacgt gctttcgcta ccggcctgcc ggtatttatg
    26941 gtgaacacca acacctggca gacctctctg agcctgcaga gcttcaacct ggaagttccg
    27001 gttgacgatc acgaacgtat cgagaaagtt caggaatacg ttgctaacta catcaacgct
    27061 gactggatcg aatctctgac tgccacttct gagcgcagcc gtcgtctgtc tccgcctgcg
    27121 ttccgttatc agctgactga acttgcgcgc aaagcgggca aacgtatcgt actgccggaa
    27181 ggtgacgaac cgcgtaccgt taaagcagcc gctatctgtg ctgaacgtgg tatcgcaact
    27241 tgcgtactgc tgggtaatcc ggcagagatc aaccgtgttg cagcgtctca gggtgtagaa
    27301 ctgggtgcag ggattgaaat cgttgatcca gaagtggttc gcgaaagcta tgttggtcgt
    27361 ctggtcgaac tgcgtaagaa caaaggcatg accgaaaccg ttgcccgcga acagctggaa
    27421 gacaacgtgg tgctcggtac gctgatgctg gaacaggatg aagttgatgg tctggtttcc
    27481 ggtgctgttc acactaccgc aaacaccatc cgtccgccgc tgcagctgat caaaactgca
    27541 ccgggcagct ccctggtatc ttccgtgttc ttcatgctgc tgccggaaca ggtttacgtt
    27601 tacggtgact gtgcgatcaa cccggatccg accgctgaac agctggcaga aatcgcgatt
    27661 cagtccgctg attccgctgc ggccttcggt atcgaaccgc gcgttgctat gctctcctac
    27721 tccaccggta cttctggtgc aggtagcgac gtagaaaaag ttcgcgaagc aactcgtctg
    27781 gcgcaggaaa aacgtcctga cctgatgatc gacggtccgc tgcagtacga cgctgcggta
    27841 atggctgacg ttgcgaaatc caaagcgccg aactctccgg ttgcaggtcg cgctaccgtg
    27901 tttatcttcc cggatctgaa caccggtaac accacctaca aagcggtaca gcgttctgcc
    27961 gacctgatct ccatcgggcc gatgctgcag ggtatgcgca agccggttaa cgacctgtcc
    28021 cgtggcgcac tggttgacga tatcgtctac accatcgcgc tgactgcgat tcagtctgca
    28081 cagcagcagt aacctcgtca tcatccgcag ctttgcgctg cggatatctg aaccggaaat
    28141 aatcactatt tccggttttt tattctctta atctgcatta atcctttctg attatcttgc
    28201 ttaactgcgc tgcatcaatg aattgcgcca tttcacttta catacttacc actttgtttt
    28261 gtgcaaggga atatttgcgc tatgtccgca atcactgaat ccaaaccaac aagaagatgg
    28321 gcaatgcccg atacgttggt gattatcttt tttgttgcta ttttaaccag ccttgccacc
    28381 tgggtagttc cggtggggat gtttgacagt caggaagtgc agtatcaggt tgatggtcaa
    28441 acaaaaacac gcaaagtcgt agatccacac tcatttcgca ttctgactaa cgaagcaggc
    28501 gaacctgagt atcaccgcgt acagctgttc acgacgggcg atgaacgccc gggcctgatg
    28561 aacttcccgt ttgaaggatt aacctcagga tcgaaatacg ggacagctgt tggcatcatc
    28621 atgtttatgc tggtgattgg cggcgcgttt ggcattgtga tgcgtacagg aaccattgat
    28681 aacggtatcc tggcgcttat tcgccatacc cgcgggaatg aaattctctt tattcctgcg
    28741 ctgtttattc tgttttcact tggcggcgcg gtatttggta tgggagaaga ggccgtcgcc
    28801 tttgccatta tcatcgcacc gctaatggtc cggctgggct atgacagtat taccaccgtc
    28861 ctggtgacct atattgccac gcaaatcggt tttgccagtt cgtggatgaa cccgttttgt
    28921 gtggtcgttg ctcaggggat tgccggcgtt ccggtgcttt ctggctccgg gttgcgcatc
    28981 gtggtgtggg ttatcgccac tatgattggc ctgatcttta ccatggtgta cgcctcacga
    29041 gtgaaaaaga atcctcttct gtcacgcgtg catgagtccg accgcttctt tcgtgaaaag
    29101 caggcggatg ttgaacaacg tccgtttacc tttggtgact ggctggtatt gattgtcctg
    29161 accgccgtaa tggtctgggt gatttggggc gtgatcgtta atgcctggtt tattccagaa
    29221 attgccagcc agttcttcac catgggtctg gtgattggca tcatcggcgt tgttttccgc
    29281 cttaacggca tgacggttaa taccatggct tcatccttta ccgaaggggc gcgaatgatg
    29341 atcgcccctg ccctgctggt gggtttcgcc aaagggattt tgctgctggt cggtaatggt
    29401 gaagcgggtg atgccagcgt gttaaatacc atcctcaaca gcattgccaa tgccattagc
    29461 ggtctggaca acgcggtcgc ggcctggttt atgttgctct tccaggcggt atttaatttc
    29521 ttcgtgacgt ccggttctgg tcaggcggcg ttaaccatgc cgttactggc accgcttggc
    29581 gatctggtcg gtgttaaccg tcaggttacc gtgctggcat tccagtttgg tgatggcttc
    29641 agtcacatca tttacccaac ctcagcttcg ttaatggcaa ctctcggtgt gtgcagggtg
    29701 gacttccgta actggctgaa ggtgggcgcg accctacttg gactgctgtt tattatgtcc
    29761 agcgtcgtag tgatcggcgc tcagttgatg ggctaccact aaaaatgtta agagccgcaa
    29821 ttgcggctct tttttcattc tgccgtttca gtctctacgg cttcattttt ggcattgcgc
    29881 ttcatccaca acgccagcgc tttcagcgag tctggagtga actcatcgca gcgtgcggtg
    29941 atttcttccg gcgtcagcca gcaaacttca ctgacttcat cttcctgtag ggcgaaggga
    30001 ccgtgagaga cgcagctgaa caacgcgccc cagacacggc aatttttatc ttcgaaatag
    30061 aactgcccgt gctcggcaaa ggggacaccg gcaatgccca actcttcttc cgcttcgcga
    30121 cgcgcggatt ccagcagttg ctcatcggcc tgaaccacac cgcctgcggt cgcatctaac
    30181 atgccgggta aaaagtcttt tgtctcggta cgacgctgga ccagaatttt gcccatgcca
    30241 tcatgcacga cgatgtaagt tgcacgatga cgcagacact gtgcccgcat ttgttcccgg
    30301 ctggcttgtg caatgacttc gttctcttca ttgacaatat ccacccattc agtacttgcc
    30361 aaacgacgct gttccattat caggaaacct tctttttctg gcgctcttac ggcgcatttt
    30421 gaattgtggg taaattacgg attaatcgcg acctgcgcaa tgatactttg atcattgagt
    30481 gcgattacac taagtacgtc attatccagc atgccataac tcgccggatt accgcctttc
    30541 ggaatactca ccgagccggg gttgaagtgg aaaatctccc cccgctgttc tgccactggc
    30601 agatgggtat gaccgtacac cagcacatcg ttctggttta aagcaggtag attttccggg
    30661 ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt ccagtaatac ctgttgccac
    30721 ggcgcggtta tcgggaaatg cagcagcatt tgatccactt cgctgtcgca gttgccgcgc
    30781 acagcgataa ccttatgtgc cacttcatta agccgttcag cgactttggc tggcgcgtaa
    30841 ccctccggta aagcattacg cgggccgtga ttcaacacgt cgccaaggat caccagccac
    30901 tgggcaccgc tttgggcaaa caactccaga acacgttccg tcgccggtaa cgacccatga
    30961 atgtccgatg caaacatcag tttcatcact cactcctcgt cgaaaaaacg taagctatga
    31021 tactggattc tgtcgttgct atcagccaga ttgcttcgcc gaaagtgcaa taaaacgctg
    31081 gacagacgct cgctgccact ggaatgtcgc ataatccacc aggcgttctg gcacctcatc
    31141 gccatgtagt accaggcggt taatcattag cgccagatca gtatcagcaa tgcaccattc
    31201 accaaataaa ttcggctgac ccagtgctaa caaatgttct gccatcgcga acagtttctc
    31261 tgcactggct tttccctcgg ccgttagtgg cgcttttttc gcccccgcaa agacaacatc
    31321 cgtcggacgc tcttcgcgga tgggcatcag atcgctgcgc agccaggcct gaatccgtcg
    31381 tgcacgcgca cgattttcta aatcaagcgg ataaatacgt tcccaggtcg gtggcgcaaa
    31441 tcgatcttcc agatactccg caatggcaga agattcactc aactcaaaat catcgatttg
    31501 taataacggc acacggcgtg tctgaccgta accttgccac gtcggcttca aatgttcacc
    31561 gctgtcgagg tcgatggtct tgatatgaaa cgacaggcct ttttcctgca acgccaccca
    31621 ggcggataac acataagggg agaaaaagtg ggcatctgac caaagcgtga tagcgggttt
    31681 actcattatg tcctcggcaa taaagggggc tggctatcaa actatagagc ctttttgtca
    31741 ctgtcacgtg acgaatactc acgtaagtcg ccgcgctttc tatacttgtg gtgttcagat
    31801 gattaaacaa ccggagctgc aatgatcgat ctctatttcg ccccgacacc caatggtcac
    31861 aaaattacgc tgtttctcga agaagcagag cttgattatc gcttgattaa ggtagacctg
    31921 gggaaaggcg gtcagtttcg tccggaattt ttgcgcattt cgcccaacaa caaaattccg
    31981 gcaattgttg atcattctcc tgccgatggc ggcgaaccgc taagcctctt tgagtctggt
    32041 gccattttgt tgtatctggc tgagaaaaca ggactctttt tgagtcatga aacgcgtgag
    32101 cgcgccgcca cattacagtg gttattctgg caggtaggcg gactggggcc gatgcttggg
    32161 caaaatcatc attttaatca cgcagccccc caaaccattc cttacgctat tgaacgttat
    32221 caggttgaaa ctcagcgtct ttaccatgta ctaaacaagc ggctggaaaa ctcgccctgg
    32281 ctgggaggcg agaactacag cattgcggat attgcctgct ggccgtgggt taatgcctgg
    32341 actcgccagc gaattgacct cgcaatgtat ccggcagtca agaactggca tgagcggatc
    32401 cgttcgcgcc ctgccaccgg gcaggcactg ctaaaagcac aactcggtga tgagcgttcg
    32461 gatagttaac agaaacaggt tctcgtgtat tatttcatcc taagtaaaac aacggagaac
    32521 ctgcaatggc acaacctgcc gctattattc gtataaagaa ccttcgttta cgtacgttta
    32581 tcggaattaa ggaagaagaa attaacaacc gtcaggatat tgttatcaat gtgacgatcc
    32641 actaccccgc cgataaagcg cgcactagcg aagatatcaa cgatgcgctg aattatcgca
    32701 ccgtaacgaa aaacattatt cagcatgtag agaataaccg tttctctttg ttggaaaaat
    32761 taactcagga tgtgctcgat atcgcacgtg aacatcactg ggtgacgtat gctgaagtgg
    32821 agatcgataa actgcacgcg ctgcgctacg ccgattcggt atccatgacc ttaagctggc
    32881 agcgttaacc gccatatcgg gaggctgcat gaacatagtg atcaccggag ggacgggatt
    32941 aattggtcgc catttgattc cgcgtttgct ggagctgggc catcaaatca cggtagtgac
    33001 gcgtaaccca cagaaagcca gttccgttct cggccctcgg gtgacgctat ggcaagggct
    33061 agccgatcaa agcaacctca acggcgttga tgcggtaatc aacctggccg gagaaccgat
    33121 tgctgataaa cgctggactc acgagcaaaa agagcgtctc tgccaaagcc gctggaatat
    33181 cacgcaaaaa ctggtcgatt tgattaatgc cagcgacacg ccgccgtcgg tactcatttc
    33241 cggctcggca acgggctatt atggcgactt aggtgaagtg gtggttaccg aagaggaacc
    33301 gccgcataac gaatttactc ataaactctg cgcccgctgg gaagagattg cctgccgggc
    33361 gcaaagtgac aaaacgcgag tgtgcctgct gcgaaccggc gtggtactgg caccggacgg
    33421 cggtattctc ggtaaaatgc tgccaccgtt tcgtcttggc ctgggcgggc cgattggttc
    33481 cggtcggcag tatctggcct ggattcatat tgatgatatg gtcaacggca ttctctggct
    33541 gctggataac gacctgcgcg ggccatttaa tatggtttcg ccctacccgg tacgcaatga
    33601 acaatttgcc catgcgctcg gtcaagcgct gcatcgcccg gccattttgc gcgtccccgc
    33661 aaccgccatt cggctgttaa tgggcgaatc ttcaatactg gtattaggtg gacaacgcgc
    33721 gctgcctaaa cggctggaag aagcgggttt tgcgtttcgc tggtacgatt tagaagaggc
    33781 gctggcggat gtcgttcgct gatgtggttt acagcaaaca tccgccagtt aactcccggt
    33841 gttacaggat tagtggcttt gcgcgataag atcgtctggt gaaagtcggg tcaccatcat
    33901 aactaactct ctgtctaaac ctctatccag catctcctga gcaatacgca gggcttcttc
    33961 gtgtttgccc tgcattgcgc cttcttcacg taatctgtca gcaatggtca tcagtttctc
    34021 cttttcttgt ggtgcgcgtt ctgttatctc accaataaat gcacggaaac gctgggcatc
    34081 ccctgtttgt aatacgtaat taaacagggc ttttagctgt ctgtcattag tgttccctgt
    34141 aactagcagc gaaacaattt ggtcgactaa tcccaacaga tcgcgctgac gaatatgttt
    34201 ctgaattaac tccaacagcg ccattttgcg gtgttgcata atctcgtcat ccggcaccac
    34261 ggtaatatcc accaacggaa acgctgatga atatattttg cgggctatag caggctcggc
    34321 aaattcatca agccagcaga gtgaataagg ataaggactt ctgcaaccat gataaaacag
    34381 catcgggatc accaatggaa gctctttata gcccgcatca agatggtttt gcattgccgc
    34441 aatggaataa cgcatcatgc gaaaagccat taattcttcc agcttacttt ggtgctctat
    34501 cactacataa atataaccca ctccctcctg cgttttcaca gaccacaaga ggtcggaata
    34561 atattgccgc aggtcttcat caataaaact gtttgattct agtttaagcg tcgttaaatc
    34621 acacagtttg cgcagcggcg cgggaagatg aatatcaata aaatcccgcg cggtgtctgg
    34681 atggcgtaaa aaagatttaa ataccgcatc atgcggcgtg gaagttgtcg atattgtcat
    34741 ggcgttccgt cacccctcaa atttaagatg acgcgacaat aaccacgatt aacacccaat
    34801 gcagcaggca gtttttatct ttggacagcg aattcagaag tatttttagc ggactgcaac
    34861 ttttacattt cagcggaagc tgactcgcaa attccccatg aaaatactcc ggcagcgtta
    34921 gccaccggag aggaaattat ttcagcgatc ccttaaggaa ctgttgcaga cgagggcttt
    34981 gcgggttgcc aaataactgc tccggcgcgc cctcttcttc tattttcccc tgatggagga
    35041 aaatgacgtg agtagaaaca tggcgagcaa agcccatttc gtgagtcact accaccatgg
    35101 ttttcccctc ttctgccagt tgctgcataa tacgtaacac ttcgcctacc agttcaggat
    35161 cgagcgccga ggtaggttca tcaaacagta aaacttccgg ttccatcgcc agcgcccgcg
    35221 cgatagaaac acgctgttgc tgaccgccgg aaagatgcac cggatatttc ccctgcgcac
    35281 gttcgtctat cccgactttc gccagatact tcaccgcccg ctcgcgcgct tcctgcttgc
    35341 tcaggcccaa cacctgaatc ggcgcttcca tgacgttttc cagcaccgtc atatggctcc
    35401 agagattgaa gtgctggaat accatcgtca ggcgtgtgcg cagtaagcgc agttgatttt
    35461 tatcggcgac tttgagttga ccgtctttgt cgcgcaccag attgatcgtc tggccattga
    35521 ccacgatcga cccttcactc ggtttttcga ggaagttaat gcagcgcaga aaggtacttt
    35581 tccccgatcc cgacgatccg atgatgctta ttacatctcc ggcattcgct tgcagtgaga
    35641 cccctttcag cacttcatgt tcgccgtagc gtttgtgcaa atcgataacg tttaatttat
    35701 tctcggacat cgtattctca gtgcgttgaa gaaggtttca catgctgcaa ccagcgtttt
    35761 tccgctctgc gaaagaggct gatcaggaca taagagatga ttaaatagag caccgcggca
    35821 atgccgaagg cggtaaaagg ttgatacgtg gcggcgttaa tatcgcgggc tattttcagc
    35881 agatccggca cagtggccgt aaacgccagc gcagtagagt gcagcatcag gatcacttcg
    35941 ttgctgtatg ccggtaacgc aatacgcagc gcagaaggca aaataatgca acgatacatt
    36001 ttaaaagtcg agaagccata ggctctggcg gcttcaattt ccccatgcgg aaccgaacgg
    36061 attgccccag caaaaatctc ggtagtgtaa gcgcaggtgt taagcgtcag cgccagcacg
    36121 gtacagttca ggccactgcg gaagaaagcg ttaaggaatt cggttccctt aacaatctca
    36181 agcgtgtaca tgccggaata gaacaccagc aactgaacat acagcggcgt accgcgaaaa
    36241 atataggtaa ataaccagat tggaaactgg atgtatttat tactggagac acgaccaatc
    36301 gccagaaaca gcgccaggac tccgcctatc actaccgaca aaataagcag ccacagagtg
    36361 atcgccacac cagtaaagcg ataaccgtcg gtccacagca gcggtttcca gtattcatgt
    36421 aagatttcga tcacaggtca gccctcttca cacccacgga gtagcggcgc tcaaggaaca
    36481 gcagcacacc attggaaaca gtggtgaaaa ccaggtaaat cacgccacag acgatggcga
    36541 aatagaacgg ttcccaggta cttttgcctg ccagttgggt ggctttgacc acatcttcca
    36601 ggccgagtaa cgaaaccagt gcggtagatt tgaggatcac ctgccagttg ttgccaatgc
    36661 ctggcagggc gtaacgcatc atcgccggaa acatgatccg ccgaaacact tgcccacgag
    36721 taaaaccgaa cgccgtcgcc gcctctatat gtcctttcgg cactgccata aaagcaccac
    36781 ggaacgtttc ggtaaaataa gcaccgtaga taaaaccgag agtgataata ccagcgacca
    36841 tcggatcgat atcaatctgc ccgacgccca tcgcctccgt caccgtgttt agcgcaatct
    36901 gcaaaccgta gaaaatcagc agcatcaaca ctaagtccgg cacgccacga atcagcgtgg
    36961 tgtacccttc gaaaatcagc cccgaaagcc gattttgcga gagcttaccg ccagcgccaa
    37021 ttaaaccgat gattacagcg agcactacag agctgatagc cagctccagc gtgacgagcg
    37081 caccctgtaa aataacacct gaaaacccat acaacatgct gcctgtcctg tcgtgtgtgg
    37141 tgaattaccg cctcttgtct ccctccggtt acccgaaggg agcgggcttt ctttcaccga
    37201 tggcgattag ccaccataaa catcaaaatc gaagtacttt ttcgctaatt tctcgtaagt
    37261 accgtcagcg cgcatttcgg caaaggcttt gttcagtgct tcgcgcagtt cgttatcttc
    37321 tttacgcagg cccatgccgg tccctacgcc aaacagtttt tcatctttaa cagacgggcc
    37381 accgaatttg taatctttac cgacaggttg tttgaggaaa ccttcgctgg cagcgacctc
    37441 atcctggaac gcggcatcaa tacgtccggc agtcaggtca gaataaatgt tgtcctgccc
    37501 ctgatacgag acgatttcaa tgccttttgg tgcccaatgt tcattaccga acgtctcctg
    37561 ggtggtgccc tgcaatacgc ctacccgttt gcctttcagc gactcgactg tcggctgaat
    37621 gtcagaattt ttcgccacca ccaaacgaga atcggcagcg tacagtttgt cggtgaaggc
    37681 tatttcttgc tgacgttttt ccgtaatgga aagcgatgac atgatggcgt caatcttctt
    37741 cgcttttaag gacgggatta acgcatccag cggattttcg acaaacgtac attgcgtatt
    37801 gatgcgtttg cataattcct ttgccagatc gatatcgaag ccaaccagtt cgccttgtga
    37861 attttttgat tcaaatggcg cataggtcgg gtcggtaccg atgcggatgt tttgcggaat
    37921 cgcagcaaat gccgcagttg cgctggagaa ggccagaacc agagagagcg atagcaccag
    37981 ttttttcata atgtcctcaa ctgacagtct ttttataggg aatatttaca ggtttgttac
    38041 acttatcgtg ccataaaatt gaccatcaag gcaaaatatt ccgccccgtt agacgtatta
    38101 tttttcatta aaaatgctta agtatgcatt tcaatgcacc acgatagtgc aacaaaccta
    38161 gcgtgcacca tgatgatcat tccatcaggt acagcttccc agcgacgtat cagtcaccgt
    38221 agacattaaa gtcgaaatac tttttcgcca tcttgtcgta ggtgccgtcc tgacgcagct
    38281 cgccaagcgc cttattgaag gcagccgtca gttcagcatc atctttacgt agccctacac
    38341 cggtgccatc accgaagtat tttttgtctt ttactgatga gccagcaaag gcgaaagctt
    38401 taccagcagg ttgcttgagg aatccttcgc tggcagcaac ttcatcttgt aacgcagcat
    38461 ccagacgtcc tgcagccaga tcggaataga ccaaatcctg gttggcatag gccaccacat
    38521 ccacgccttt gctacgccag gtctcgttag cgtaagcttc ctgggttgat ccctgcagca
    38581 caccaacatg tttacctttc agtgaatcca gcgttggctg aatcggtgaa cctttggcag
    38641 caatcaaacg agaatctgcg gcgtacagct tgtcggagaa ggcaatctcc tgctgacgtt
    38701 tatcggtaat ggaaagcgac gaaataatgg cgtcgatttt tttcgctttc agtgagggga
    38761 tcagcgcgtc aaagtcactg gcaacccagg tacatttcac ctgcatccgt ttgcacatct
    38821 cgttaccgag atcgatatca aagccaacaa aatcaccttt agcatctttc gacgagaacg
    38881 gcgcataggt ggtatcggtt ccgatacgta ccgtctccgg tagcgccgca tagctggaag
    38941 ccgctgcgga gagaccgact aacaaagaca gagcgagaat cgacttcttc atacataacc
    39001 ctcaagtgaa aatggctttt ttatgttgtg tattgtgttg tgtgtttgca ggctctttca
    39061 tgcaggtctt atgccatctt gacgacaaca gtaacattca acgttaaata tgttaataag
    39121 acgttgcatt attgtcctga agttgaagat agcaggtata gcggttgaat cgcagcgttt
    39181 cgattgaatg gcagaaacaa aatgtcgagg atttgatcgc ggttgcaaaa ttgccctgaa
    39241 acagggcaac agcggagtta tgcgccctgc cagcgggcaa agagatcttc aggaagggtt
    39301 attgcaaact ggtcaagaac acgattaacc gtctgattta tcacatcatc aagggattgc
    39361 gggcgatgat aaaacgccgg aacgggaggc ataatcaccg caccgatttc tgccgcctga
    39421 gtcattaaac gcagatggcc taagtgcaat ggtgtttcac gcacgcagag caccaacggg
    39481 cgacgctctt tcagcaccac atctgccgca cgggtcagta agccatcagt atagctatgg
    39541 acaatgccgg aaagggtttt gattgaacag ggtaaaatca ccatccccag cgtctggaaa
    39601 gaaccggaag agatgctggc ggaaatatcg cgcgcatcgt gcgtgacatc agctaatgcc
    39661 tgcacttcgc gcagggaaaa atccgtttcg agggataagg tctggcgcgc cgcctggctc
    39721 atcaccagat gcgtttcgat atctgtgaca tcgcgcagaa cctgtaataa gcgcacgcca
    39781 taaatcgcgc cgctggcacc gctgatgcct acaatgagtc gtttcataaa tagttgccct
    39841 gtcagacttg caggcagact ttgcagtatt tcggcgggag ttgcaagtca gggtgccaga
    39901 ccggcaccct cagcgaaggc atcatccttc gttatgcatt tcgagatttt ccacttcgtt
    39961 ctgacgttgc actgctttgg cgtcatcatt acgtaacgta tcgaggaaat cgaggtagcc
    40021 ctgatcaaca tctttggtga cgtagacgcc gttgaacacc gagcattcaa actgctggat
    40081 atccggattt tcagcgcgaa cggcgtcgat cagatcgttc agatcctgga aaatcaaccc
    40141 gtcagcaccg atgatctggc gaatttcatc aacttcgcga ccgtgagcga tcagttccgt
    40201 ggcgctcggc atatcaatac cataaacgtt cgggaagcga atttccggtg ccgcagaagc
    40261 gaggtacact ttcttcgctc cggcttcgcg tgccatctcg ataatctgct cagaagtggt
    40321 gccacggacg atggagtcgt cgaccagcag gacgttttta tcgcggaact cggcgcggtt
    40381 ggcattcagt ttacggcgca cggacttacg acgcagctgc tggcccggca tgataaaggt
    40441 gcggccaaca tagcggtttt taacgaagcc ctggcggtac ggtttgccca gaatacgagc
    40501 aatttccagc gcgatatcac acgaggtttc tgggatcggg atcaccacgt cgatatccag
    40561 atcttcccat tcgcgggcaa ttttctcgcc cagtttcgtg cccatattca cacgcgcgct
    40621 gtaaacggaa attttgtcga tgaacgagtc cgggcgagca aagtatacat actcaaacag
    40681 gcacggattg ctgaccggat tgtcagcaca ttgacgggta aacaactgcc cttcttcagt
    40741 gatgtaaatc gcttcgcccg gcgcgacgtc acgcaggaaa tcaaagccca gcgtatcgag
    40801 cgctacgctt tcggaagcga ccatatattc tgtacggttc tcgtcaatat cacgttttcc
    40861 cagtaccagc ggacgaatcc cgtttggatc gcggaaagca accataccgt ggccgataat
    40921 catcgccaca caggcatatg cgccgcggat taagcggttt gtggcagcaa tggcagcgaa
    40981 aatattgtcg gcttccagcg ggtagtggcg gaagttgtcc agctcgctag cgaagatatt
    41041 aagcagaatt tccgagtcgg aagtggtgtt gatgtggcgg cgtttttctt caaacagttt
    41101 tttacgcaac tcgtgagcgt tggtcagatt gccgttgtgg gcaagcgtaa tgccatacgg
    41161 ggagttaacg taaaacggct gcgcttcaga ggcgctggag ctgccagccg tagggtaacg
    41221 cacatgacca atgcccatat tgccctgcaa acgctgcata tggcgagctt caaatacatc
    41281 gctcaccagc ccgttcgctt tacgcaaacg gaagcagtta ttggcatcta tggtgatgat
    41341 gccggcggca tcctgaccgc gatgctgaag caccgttaag gcatcataaa tcgactggtt
    41401 aaccggcata acaccggcga taccgacaat accgcacata cgtctttttc ctcgttaagc
    41461 cacatctcag agcacttacg ctctgggcaa gaaacttgac gagctttgca gataatcaaa
    41521 aaaccatctg atgataaaac tgaactgcgg gatcagctgt gatttgctcc agtcttcgct
    41581 tttcgacacc ccggtaaagg agtcgagaaa gaagagaatg gcggcaacaa tcaacacacc
    41641 gcgcaacgca ccgaaacaga cgcccagcac ccgatcggtg cctgacaacc ccgttttctc
    41701 caccaactgg cctatcacga agttcacgat agcaccaaca atcagggtag cgataaacag
    41761 taccgcgatg gcaattccat ttcgaaccag ttcgtcttca aagcccgtaa accagactga
    41821 caggtaagtg tagtaatgac tggcaacaaa gaaagcacaa ccccatgtca ccagcgataa
    41881 cgcttcacga acaaagccgc ggatcaggct aaccagagag gaaaaagcaa tcaccgcgat
    41941 tatggcgtaa tcaatccaga ccatatgtgt cccacgattt tacgccctgt catcctgttc
    42001 ggggcgcatt ctaacagaaa aagaaaacgt ttgcgtaggg atttccttcc cacgcatcaa
    42061 taaaaatggc gctgaaaaaa tattcaacgc catcgacttt ttatgccttt gcggcatcgt
    42121 gcaatgcgtg tcggatgcgg cgtaaacgcc ttatccgacc tacggttcta acactgccca
    42181 ggcctgataa gacgcgccag cgtcgcatca ggcaagactg tattaattcg gcgtatagcc
    42241 cattaccacg ccacttaagc cagaaagttg cttcaactca cccagcgaac ctttcagctt
    42301 atctttcgag gcatccggcc caaccagaat acgggtaatt ttaccctgca ctggcgtgga
    42361 tggcgacgta taaacccgat aaccggcacc gcgcagctta ccgacaatct cattcacttt
    42421 atcggcattt ttcagcgcac ccagttgcac aacataggct ttacccgttg gcgctgcttt
    42481 ttcttccacg accggcttcg gttctggctt cggcgcaggt ggtgcttcaa ccttcggttt
    42541 aggcggctcc accggtttcg gcttcggtgg ggcgaccggt gcaggttccg gttcaaactc
    42601 ggtgttattg gctgcaatag tggccggatc gagcgacggt gctgcggcat cacctgcccg
    42661 cacctcttcc gctgcgcctt ccggcggctg cgtcggcaac gcctgggtgg cggctggcat
    42721 catatcaggc tcatcgcgat cgcccgcttt tggaaccagc gggatagccg caaactcatc
    42781 ctgataatgt tttttctgcc cgtccagcag ccctggaagt acaatcaccc ccagcgccac
    42841 cagcacgatc gtgcccacta accgattctg aaacttactt gccaccgctt ctcctcgcgt
    42901 caatcacttc catgacatgt gcgaccgtgt ggaaagaacc acacaccagc acggtgtctt
    42961 ccgctttagc gtccgccatt gcggcatccc atgcctgcgc aacgctatca aatgatttgc
    43021 cgttacccaa atgctcaagc agttgttctg ccgtggcacc gcgcggccct tccagtggcg
    43081 cacaatacca gtcatcaacc acgcttttca accaggccag agttccggca atatctttat
    43141 catgtagcat accgataacc gccagcacgc gcccgttttt cggtagcgct ttcatacgcc
    43201 cggtgagata ttccgccgca tgtggattat gcgcgacatc aaaaataacg cgtggcgact
    43261 cgctcacaat ctggaaacgt cccggcaaaa ttgcgctggc aatcccgtcg cgaatggcat
    43321 tttcactgac ttccagcccg ctggcacgca gtgccgccag cgctgttgcg gcattcggtt
    43381 gcgggacaag cggcaacggc agattttcca gcgtgccgtg agcatcgcta aacgcccagt
    43441 catgatcggt gacggaatag ttccactcaa cgccccgacg ttgtaacagt gcaccttttt
    43501 cctgcgccac atcagcaatg gtagaaggca tttccggctc accgacaatt gccggttttg
    43561 cgctgcgaaa gatgcctgct ttctcgcgac caatactttc gcgatctggt cccagccagt
    43621 cggtatgatc cagcgcaatg ctggttacta cagccacatc ggcatcgaca atattggttg
    43681 cgtccagacg accgcccagc cctacttcca gaatcactac gtcaagttgt gcctgtttga
    43741 acagccacaa cgccgacagc gtaccgtact cgaaataggt cagggaaata tcaccgcgtg
    43801 ccgattcaat ctccgcaaaa gaggcggtgt gggccgattc cggcaattcc tgcccctgca
    43861 cacgtacgcg ctcggtataa cgcaccagat gcggtgaact gtagacgccc actttgtacc
    43921 ctgccgccat cagaatcgac tccagcgtac ggcaggtggt gcctttgcca ttcgtacccg
    43981 caacggtaaa cacaaatggc gctggtttca ggacgccaag acgcgccgcg acctggctca
    44041 cgcgctcaag gccgagatcg atagttttac tgtgcaggtt ttccagataa gaaagccacg
    44101 aagccagagg cgacgcggct tgaggagtgc gtttgataat catggtatcc gctgattcgt
    44161 tacggtgaga atagcaaaag ggcagagcca gtggccctgc ccttatcagt tatcaggcct
    44221 caggttcctg atccggtacc gggggtacca ctacgccttc acgcggcgct tcaggattcg
    44281 gcgctggcag attcatcaac ttcgccagaa tgctcgccag tttcaggcgc atttccggac
    44341 gatggacgat catgtcgatg gcgcctttct cgatcaggaa ttcactgcgc tggaatcctg
    44401 gcggcagttt ttcgcgaacg gtctgttcga taacacgcgg accggcaaag ccaattaacg
    44461 ctttcggttc agcgatgttg agatcgccca gcatggcgaa acttgcagaa acaccgccca
    44521 tcgtcgggtc ggtcagcacg gagatgtacg gcaagccgcg ctcctgcatt tttgccagtg
    44581 ccgcagaggt tttcgccatc tgcatcagcg acatcagtgc ttcctgcata cgtgcgccac
    44641 cagaggcgga gaagcagatc agcgggcagt tatcttccag cgcctgctca acggcacgca
    44701 cgaaacgtgc acccacaaca gaccccattg aaccgcccat aaaggcgaac tcgaatgccg
    44761 cagcgacaac cggcattcca tacagagtgc ctttcatcac caccagcgca tctttttcgc
    44821 cggtttcttt ctgcgcagaa gccagacggt ctttatactt cttggagtca cgaaacttca
    44881 gcacatcttt cggctcaagc tcgctaccca gctccacaag gcttccttca tctaacaggc
    44941 tatgcaggcg attacgcgct gtcatacgca tgtgatggtc acacttcgga cagacctcaa
    45001 gattacgttc cagctcagcg cggtataaaa cctgaccgca gctatcacac ttagtccaca
    45061 ccccttcagg aatgctcgcc ttgcgggtgg gagtaatgtt gcttttaatt cgttcaatcc
    45121 agctcattag ggacctttct gtctgaacct ggttcgatgc cagttttatc tttggggacg
    45181 cataatgcca tttttgcccc caacagacca tgaatgttgc acattaaaac ataacagccc
    45241 gaaactttgg ataaaaaagt ggtcgaaccg cggagttact ttttattttg cggcgcgtgc
    45301 cgcagcgcgt ttgtgacgaa taaattcgaa aaccccaggc aatattgaaa caaaaataat
    45361 gccaacaatc atcagcttaa ggttgctctg aatgaagggg agcgtgccga agaaataacc
    45421 cgcgtaggta aaaatcagta cccacaacag tgcgccgatc acgttataag cggcgaaatg
    45481 acggtacgac atgtggccca ttcccgcaac aaacggcgcg aacgttctga cgatcggtac
    45541 aaaacgggcg agaataatcg ttttgccgcc atgtctctcg taaaactgat gagtcttatc
    45601 aagataactg cgacgaaaga ttttcgaatc cgggttactg aacagtttgt caccaaataa
    45661 ccgtccaatg gtatagttta ctgcatcacc gacaatagcg gcaatcaaca taagcaccac
    45721 catcatatgg acattgagat cgttagtttc cagcgacgcc aatgcaccag caacaaacag
    45781 cagcgaatca ccaggcagga atggcgttac caccagcccc gtttcgcaga acagaatcaa
    45841 aaacagaatg gcataaaccc agacgccgta ctccgcgacc agctctgcca gatgcacgtc
    45901 aatgtgcagg ataaaatcga tgaggaaata aatcaggtcc atatttgcct atgccttgta
    45961 ctcgtcatct ttcaggctgt aactacgttg gctacagcct gaaatgttcc gagcattatt
    46021 ctcgttagtc cgccagaaat agcgggccca ttggcggttt tggaagatca taccggtcag
    46081 ggtaatccac cgcgaccaga tacagccctt ccgcttttgc cgttgctgcc gccagcgttc
    46141 tgtcctttgc cgccagcaac tctgctatcc atctctccgg ctggttgtgg gcaccgactt
    46201 ccatcaggct gccgacaata ttcctgacca tatgatgtac aaaggcattc gctttgatat
    46261 ctaccaccac ataaggaccg tgacgcgtga cattaatatg cataacgttg cgccacgggg
    46321 ttcgggactg gcactgcacc gcacggaacg aggtgaaatc attctcgccc agcaagcatt
    46381 gcgcagcccg atgcatccgt tcagcgtcca gcggttcgta aaaatgggtt acccctttac
    46441 tcaataccgc cgggcgcagc cgatgattgt agatgatgta gcgataacgg cgagccgtgg
    46501 cgctaaatcg ggcatgaaaa tcatcaggta cagctttaac ccaacgcacg gcgatgtcac
    46561 caggtaaatt cgcatttacg cccaaggtcc acgccgcgtc tttgcgctgc gcggttgttt
    46621 cgaaatgcac cacctgcccg gtaccgtgta cccctgcgtc agtacgcccg gcgcagaaga
    46681 cggtgatggg ttcgttcgcc acctgggaga gcgccttttc cagcttctcc tgcacactgc
    46741 ggacttcatt ctgccgttgc cagccgtaat acttactgcc gtcgtactca atgcccagcg
    46801 caattttata aactggcagt tgttgctggt cggacattag tacagatact cctgcaccag
    46861 tttctcggcg atttttactg ccatcagcgc gccgccaaag cgaacgttat cggccaccga
    46921 ccagaactgg acttgctccg gcataccgta gtcattacgc acgcagccaa cagaaagatg
    46981 cggcgtaccc gaagcatccc ctacctgagt cgggaattcg ttctcttcag agagcacaat
    47041 atcttcgccc tgagcaaacg catcacgcgc ttcttctgcc gccagcggac gcagtgcttc
    47101 aaagttgacc atctgggcat gaccgtagaa taccggtgcc tggacgacgc ttgccgaaat
    47161 catcagccct tcgtcctgca ggattttgcg tacttcgtca acgatacgac gttcttcacg
    47221 cacgctacct tcgctatccg gcaacaacgg cagcatattg aacgccagtt gacgaccgaa
    47281 gaaatcttct tcgtcaatcg gaatgccgtt gagcaatttc gcactctgcc ccgctaacgc
    47341 atcgaccgct tttttgccct gggcggaggc tgaaatcagg ctggtaacac tgatacgcga
    47401 caaaccgccc tgatcgatta acggtttcag tgccgccagc agctggctgg tcagactgtc
    47461 tggtacggcg atgacattcc ggttgcggta atcggtcagt acaaacgggt ttacttccgg
    47521 caccaccagc ggtacgtcgg gttcgagagc aaacagtccg ctgctgtcga tcaccaggca
    47581 acctgagttg gtcgcttctt caacccaggc agcggtagct tctttgcctg cgacaaaaaa
    47641 tgccagctgc gcctgcgtcc agtcgaattc agcggcatcc tgcacagtga ttgttttacc
    47701 accaaagcgc agttgttcgc ctgcgctttc gttacgtgcc agtgcataaa tttccccaac
    47761 cgggaactga cgttcagcca gcgtttcaag cagggcttcg cccacagcgc cagttgcgcc
    47821 caggacggca atgttccagc cttcagacat ggtggtttac tccagaaata gcaaagctcc
    47881 ctgccaatgt tacagcagag agcatgaaga agagattaac gtgccggatg atgaacggcg
    47941 ttaaaaccca gtttacacag caatgatgcc gcactggcgt catcacaaat tacatacaga
    48001 gatgaccatt cacggcgctc aagatagttt ttgcgcagtt tatcgaactc acccggtatc
    48061 ccggcgactt tacgcagcgg tgcgtcatcg cggcgcacat catacaccaa atgcaccagc
    48121 cttttcagcg tcggttgatc gagcgggcca tgcagcgtaa tgcgaccaaa ctctggcgcg
    48181 ggcagtaatg tatccagcgc aacgtgctgt tcatgcccaa taaacttgct ataggcttca
    48241 aacacttgcg tagtaccgcg tgctttacct tccagggtat aacctgcgat atgcggcgtg
    48301 ccgatatcca cttttgtcag caactccacg ttaagttccg gttcgccttc ccagacatcc
    48361 agcactacgc ttaacttctg gccttcattc aggcaggtca gcaacgcagt attatcgacg
    48421 actgcgccac ggcaggcgtt aatcagaatc gctcccggct tcaggctacg gatcagtttt
    48481 tcatccgcca gatgcagcgt tttgtacggc ccatctttga agagtggcgt atggaaagtc
    48541 agaatatccg catgctgaac taattcatcc agcgagcgga aatcaccctc atccccacgg
    48601 tcagcgcgag gcggatcgca aagcaatgtt ttaatcccta acgcttccag tcgcgcctgc
    48661 aatcgacgtc caacgttacc aacgcccacg atccccaccg tacggtcgtg cagtgaaaat
    48721 ccatcgcgtt cggcaagcat cagtagtgag gagaaaacat attccaccac cgcaatcgca
    48781 ttacagccag gtgcagcgga aaaaccaatt cccgcctgct tcaaccatgc ttcatcgaca
    48841 tggtcggtcc ccgcagtggc agtgccaaca aatttaatgg gttttcctgc cagcaaagat
    48901 tcattcactt tcgtgaccga acgcaccatc agcgcatccg cgtctgccag ttgagcgacg
    48961 gggattggac gcccgggaac cgcggtcacc tcacccaaac ggctaaataa gtcgcgggca
    49021 taaggcatat tttcatcaac aaggattttc acgtttgtgt tacctgtatg agacgagagt
    49081 taaccggaca agtgtgccat aatctcgcgg ccaggcatac ttgcgaagat ttcaggtata
    49141 aggatacgta atgatacaac ctatttccgg ccctcctcct gggcaaccac caggtcaggg
    49201 agataacctg ccgtctggcg cgggcaatca gcctttatcc agtcagcaac gtacttcgct
    49261 ggaaagctta atgacgaaag tgacctcact gacgcaacaa caaagagcag aactgtgggc
    49321 gggtatcagg cacgatattg gtctgtcggg agattcaccg ctgctttcgc gtcacttccc
    49381 tgccgctgag cataatctgg cgcaacgtct gctggccgcg caaaaaagcc attctgcccg
    49441 ccagctttta gcgcaattag gggagtattt acgtctgggg aataatcgtc aggcggtcac
    49501 ggattatatc cgtcataact ttggtcagac gccgctgaat cagctctcac cggagcaatt
    49561 aaaaaccatt ctcaccctgt tgcaggaagg gaagatggtt attccgcaac cacagcagcg
    49621 cgaggcgacc gaccgtcctt tattaccggc ggagcacaat gcgctaaaac agctggtgac
    49681 caaacttgcg gcggcaacgg gggaacccag caaacagatc tggcaatcga tgctggaact
    49741 ttccggggtg aacgatggcg agttaattcc agcgaaactg tttaaccatc tggtgacctg
    49801 gttgcaggcg cgccagacgt taagccagca aaatacgccg acgctggaat cactacagat
    49861 ggcgctaaaa caacctttag atgccagtga actggcagcg ttatcggcgt atatccagca
    49921 aaaatatggt ctttctgcgc aatcatcgct ttcttctgcc caggccgagg atattcttaa
    49981 tcagctttat caacggcggg ttaaagggat tgatccgcgt gatatgcaac cgctgcttaa
    50041 tccttttcca ccgatgatgg acacgttgca aaatatggca acgcgtcccg cgctgtggat
    50101 actgttagtc gcgattatcc tgatgctggt ctggctggtt cgttagcccc gacgaaatga
    50161 cagtatcgtg acaataattc ccagcaccgc agagatcgcc ccggcaagaa ataccgaaga
    50221 gtaaccaaac gtggtcgcca gcattcccgc cagcggcccg gagacgccga gggcgatatc
    50281 ctgaaacgcg gcgtaaccgc ccagtgcggt gccgcgaact tgtgagggga cgcgtttaac
    50341 cacctccacg cccagcgcag gaaagataag cgaacatccg gctccggtta acgccgcgcc
    50401 cgctaatgcg acccacgcac ctggggcttg ccagagcagc aacaagccca ccgtttctac
    50461 aagcagagag acaatcgcca ctttcacgcc gccaaaacgg tccggcatcc agccaaacat
    50521 gacgcgcatc acgacaaatg cgccgccaaa ctcggtaaga gtaaagcccg ccatcgccca
    50581 tcctttgctg gcaaagtaga gcgaaacgaa agtcccgata accgcaaaac caacgccttg
    50641 tagtgccagc cctaaccctg gtttccagat aaacccgaca acgctccaca gcgatggacg
    50701 ttctcccgcc agggccggta ctttgcgcac tgtgccgtta caggcccacg ccagtaaggg
    50761 taataccatt gtggtgatcg ccagtgcggc aaaaccgtaa tggctatgaa tcaacaggcc
    50821 aagcggagca ccaacagcga gggcaccgta aatcgccatt ccattccatg acatcacttt
    50881 gccagagtgt tttggcccta cgatgcctaa cccccaggtc agagcgcctg tcagtaactg
    50941 gctttcacca aacccaagaa tcaaacgccc gatgaccaac agggcaaatt tgaacggtgc
    51001 ggagacaggc aaaatcgccg ccagcagcaa cgcgccgcca gccagaccac aagctaacat
    51061 cccctgaagc gccgaacgtt ttgcgccata ttgatcggcc agtcgcccgg catagccacg
    51121 cgtcagcacc gtagccagaa actgaatccc gacggcaatg ccgaccatgg tattgccata
    51181 gcccagttca tgatgaacaa acagcgggat aaccggcaac ggcagcccta cggtcatgta
    51241 ggtgagaaaa accgcaaaag cgatgcggaa gagcgaaaaa ttggcagaag atcgtgtttc
    51301 ggtttggctt acagcagtca tgcattactc cagaatgcag cgcaaggcga ggagtatccc
    51361 cgtctcatct ctctggtttc agggttacgg tgcgttggca ggatttaacg cgtacgtctt
    51421 ttcagaagga aatcgacaaa gcgggaagtt tgcctggaac tggtggcgat tgtcaatgat
    51481 gtgaaaaagg gaaccatcag gttccctttt gcgttagtgc cggaggccgc attggcgcgt
    51541 aacgtcggat gcgacgctgg cgcgtcttat ccgacctact ccggcctact gcgaattaat
    51601 ctttcagctt gcgcattacc agcgtggcgt tggtgccgcc gaagccgaag ctgttagaca
    51661 taacagtggt cagttcgcga tcggtcgttt cggtcacgat gttcagaccc gcagcctgct
    51721 cgtccagctc ttcaatgttg atgctcgggg cgataaagcc gtgttccagc atcagcagag
    51781 agtagatagc ttcctgcaca cccgcagcgc ccagtgagtg accggtcatg gctttggttg
    51841 cagaaatcgc cgggctctta tcgccgaaca cttcacggat agctgccagc tctttcacgt
    51901 cgccaaccgg agtcgaagta ccgtgagagt tcaggtaatc gattggggta tcaacgccat
    51961 gcatcgccat ctgcatacaa cgtactgcgc cttcgccaga cggagcaacc atgtctgcac
    52021 catcagaggt tgcgccgtag ccaacgattt cagcatagat gtgagcacca cgcgccagcg
    52081 cgtgttccag ctcttcaacc actaccatac cgccgccgcc agcgataacg aaaccgtcac
    52141 ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc gttgtattta gtagacagcg
    52201 cacccatcgc gtcgaattcg caggccattt cccagcacag ctcttcgccg ccgccagcaa
    52261 acacgatgtc ctgtttgccc agttggatct gctctactgc gttaccgata cagtgtgcgg
    52321 aagtcgcaca cgcggagctg atggagtagt taacgccatg aattttaaac ggcgtggcga
    52381 ggcaggcaga aacgccggat gccatcgctt tggtgaccac atacgggcca accgctttca
    52441 ggccgcgcgg gccgcgcatt gcgtcagcgc cgaatacctg gaaacgcggg gaaccgccgc
    52501 cagaacctgc aatcaggcca acgcgcgggt tattctggta agcttccgga gaaaggcccg
    52561 catctgcgat tgcctgctcc atagaaagga atgcataaat ggatgcgtcg ctcataaagc
    52621 gcacaacttt gcggtcaatg aggccagtgg tatccagttt tacgttgccc cagacgtggc
    52681 tacgcatgcc ggaatccttc agctcctgag agaaagtgat ccctgaacgt ccttcacgca
    52741 gagatgccag gacttcctgc tggttattac cgatgctgga aacaatgccc aggccagtaa
    52801 tcactgcacg tttcattcaa tacctctgta agtcgcacat agagtaagtt tcgaatgcac
    52861 aatagcgtac acttgtacgc cgaacaagtc cgatcagcca tttaatagag aaatttgcgc
    52921 agccttacac acatcgctaa gatcgagcca ccgcctgtaa gacgagtaac ttacgtgaaa
    52981 cactactcca tacaacctgc caacctcgaa tttaatgctg agggtacacc tgtttcccga
    53041 gattttgacg atgtctattt ttccaacgat aacgggctgg aagagacgcg ttatgttttt
    53101 ctgggaggca accaattaga ggtacgcttt cctgagcatc cacatcctct gtttgtggta
    53161 gcagagagcg gcttcggcac cggattaaac ttcctgacgc tatggcaggc atttgatcag
    53221 tttcgcgaag cgcatccgca agcgcaatta caacgcttac atttcattag ttttgagaaa
    53281 tttcccctca cccgtgcgga tttagcctta gcgcatcaac actggccgga actggctccg
    53341 tgggcagaac aacttcaggc gcagtggcca atgcccttgc ccggttgcca tcgtttattg
    53401 ctcaatgaag gccgcgtgac gctggattta tggtttggcg atattaacga actgaccagc
    53461 caactggacg attcgctaaa tcaaaaagta gatgcctggt ttctggacgg ctttgcgcca
    53521 gctaaaaacc cggatatgtg gacgcaaaat ctgtttaacg ccatggcaag gttggcgcgt
    53581 ccaggcggca cgctggcgac atttacgtct gccggttttg tccgccgcgg tttgcaggaa
    53641 gccggattca cgatgcaaaa acgtaagggc tttgggcgca aacgggaaat gctttgtggg
    53701 gtgatggaac agacattacc actcccctgc tccacgccgt ggtttaatcg tacgggcagc
    53761 agcaaacggg aagtagcgat tatcggcggt ggtattgcca gcgcgttgtt gtcgctggcg
    53821 ttattacggc gcggctggca ggtagcgctt tattgcgcgg atgaagcccc cgcgctgggt
    53881 gcttccggca atcgccaggg ggcgctgtat ccgttattaa gcaaacacga tgaggcgcta
    53941 aaccgctttt tctctaatgc gtttactttt gctcgtcggt tttacgacca attacccgtt
    54001 aaatttgatc atgactggtg cggcgtcacg cagttaggct gggatgagaa aagccagcat
    54061 aaaatcgcac agatgttgtc aatggattta cccgcagaac tggctgtagc cgttgaggca
    54121 aatgcggttg aacaaattac gggcgttgcg acaaattgca gcggcattac ttatccgcaa
    54181 ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc tggaactggc gcaacagcag
    54241 ggtttgcaga tttattatca atatcagtta cagaatttat cccgtaagga tgactgttgg
    54301 ttgttgaatt ttgcaggaga tcagcaagca acacacagcg tagtggtact ggcgaacggg
    54361 catcaaatca gccgtttcag ccaaacgtcg actctcccgg tgtattcggt tgccgggcag
    54421 gtcagccata ttccgacaac gccggaactg gcagagctga agcaggtgct gtgctatgac
    54481 ggttatctca cgccacaaaa tccggcgaat caacatcatt gtattggtgc cagttatcat
    54541 cgcggcagcg aagatacggc gtacagtgag gacgatcagc agcagaatcg ccagcggttg
    54601 attgattgtt tcccgcaggc acagtgggca aaagaggttg atgtcagtga taaagaggcg
    54661 cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa tggtaggcaa tgttcccgat
    54721 tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc agaaagatga ggcggtaagc
    54781 gcgccggttt ttgacgatct ctttatgttt gcggctttag gttctcgcgg tttgtgttct
    54841 gccccgctgt gtgccgagat tctggcggcg cagatgagcg acgaaccgat tccgatggat
    54901 gccagtacgc tgacggcgtt aaacccgaat cggttatggg tgcggaaatt gttgaagggt
    54961 aaagcggtta aggcggggta atctgctctc gcagtgtttg ccggatgcgg cgtgaacgcc
    55021 ttatccgacc tacgtgtgac gtgtaggcct gataagacgc gataagcgtc gcatcaggca
    55081 tggtgctcca gacgccgcaa cttactgctg tgacgcctgc tgaaacaggt tttcccacat
    55141 atcggtcacc agcgcctggt cacgcggcga caattcaccg gcaccaatgg ctttttccag
    55201 actctggcta acggtcgtat gcaccgcctg agcggagtgg tcatcaccac tttccagttc
    55261 tgcgatggct aatgtcaggt ggccacgcaa atacccactg gcaaacaact catcatcact
    55321 ggcatggtca accatgccgt cgattaatgc cagaatgcgt gattcaaact ccgcgatcat
    55381 cttctttcct cattgtaaaa cgtggcaccg gcttcagttg agcgcttccg gccacgggaa
    55441 ctgttccgcc gtaagttccg gcgtgtgata ataattttgt agtgctttaa tgaagcgtgc
    55501 cggacgctcg ggaatgccat ttgtcagata atccatcacc tgcgcatgta cccgccgttg
    55561 aaacacaaca cgatccggtt cgaagtcgcc ttccagattg tcgcaactga cgttaaacgg
    55621 atatcccgcc gccacacaga acaaccagtc gagtgcctgc ggcttcactt caacatcttc
    55681 aaactggctt tgcgtttggg catcacgccc atccgggcaa taccagtagc cgaaatcaac
    55741 cagctcacgg cgcgcttttc cggcaataca ccagtgcgaa atctcatgaa ttgcgctggc
    55801 ataaaagccg tgggcaaaga caattcggtt atacggtact tccgcatcag caggaagata
    55861 gatcggttcg tcgtcgcctt taatcagacg ggtattaaat tcatcggcaa agcagctatt
    55921 aaaaatttca atcaactgct catagtggtg tgtactgttc attagttcat ccccaaccag
    55981 tggaggatct cctgtccgtg gctatcgtaa agtaatttgg cactcatcac cgccgagacg
    56041 ataacaatca tcgggcggat cagcttttgt cctttgctca acaccagccg tgaacccatg
    56101 cgcgcgccga ggaactgccc caccagcatg acaaagcccg tcgcccaaat cactttgccg
    56161 ccgagaataa acagcagcag accgccaatg tttgacgttg cgttgagtaa tttggcgtga
    56221 gccgtggctt tggcgaggtt gaatccgcac agcgtaacga aggccagcgc gtaaaacgat
    56281 ccggcagccg ggccaaagaa tccgtcataa aaaccgacgc agccaccggc aattaacgcg
    56341 aagggtaaac cgtacatccg gcgctggcga tcttcttcac ccagctttgg catcagcaag
    56401 aaatagaggc cgatacaaat caccagaatg ggcaaaatct gccgtaagac atcagcctga
    56461 acgtactgta ccaacagtgc gccgctcatt gagccgacaa aggtcatggc aatattgagt
    56521 ttctgatcgc ttaaactaac cactttgcgg cgaataaagt agatagtggc ggaaatagag
    56581 ccgccgcagg cttgcagttt attggttgcc agcgcgttag cgggagacat tcccgcagcc
    56641 atcaatgccg gaatggtgag taacccaccg ccaccggcaa tcgagtcgat aaatcccgcc
    56701 agcatggcga caaaaaagag aactcccagc aacagcgggg aaaccataaa caggctatta
    56761 aacgtttcca ttagatcacg tgctcatcca gtagcgcctg gcaggaaggc ggcaacggag
    56821 gcggtgtctt cttctcaggc tttgttgttc ccggttttag aggtgcaaac cagctttgca
    56881 gttctgcccc gcaaccatcg cctgatggcg gtaaaggttg atcttcacac tccagactat
    56941 cggcagggca acgtaatcgt acatgcatat gcgcgcgatg ctggaaccag ggtcgcactt
    57001 tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag ttgttgttta atcgccggat
    57061 taacaaaaat gcgcgtgacg tctttgtcct gggcagcgag tttgatcaag ctgaaaattt
    57121 ctggcttcca cagcgtggag acaacgtgtt taccgtcgcg ggaaactaag tccagtgctt
    57181 gcgggcgcaa gagctgcgcg gaggtccagc gagttttcgg cagttgcaga aagatatcga
    57241 catccagtcc ggtctggtgg ctggcatgac cgccgttgaa acgcccacca gcgggcatcc
    57301 ccatatcgcc aatcagcacc gtacccatgc ccagattgct cacctggcta ctcagacgct
    57361 ggataaacat caccagatcc gggtgaccga aatagcgacg ctgatcggta cgcatgacct
    57421 gataatgttc ggactgtatc ggcaacgtgt cagcgccgac aatacagcca ttagaaaaac
    57481 tgcctatcga ttgtgcgcta cccggcacag gttgggttat tttttgccac ggcgtcgctg
    57541 ccaggctagc gctactggca agcagagcca gcagcgcaat cgcggttttg ttcatttttt
    57601 accagcgtgg aatatcagtc ttcacatcgg cattttgcgc ccgctgccgt aacaggtgat
    57661 ccattaaaac gatcgccagc atcgcttcag cgatcggcac tgcacggatc ccgacacagg
    57721 gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc aaagcggtta atagtgcgcc
    57781 ccggcacggt aatgctggag gtcggtttca gcgccatatg agcaatgatt tgctgcccgc
    57841 tgctgatacc gccgagaatg ccgcccgcat ggttgctctg gaaaccgtct ttggtgattt
    57901 cgtcgcggtt ctggctgccg cgcagcgcca ctacgtcaaa accgtcgcca atttccacgc
    57961 ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc ggcatccagg cggtcaaaga
    58021 ccggctcgcc aagtccggcg gggacaccac tggcaacaac ggtgactttc gcgccgatgg
    58081 agtcgccctc ttttttcagc gcgcgcatca gttcatctaa cgcgtcgatt ttgtccgggt
    58141 ccgggcagaa gaacggattt tgttcaacca gcgaccagtc tttgatttcc agcggaatgt
    58201 cgcccatctg ggtcaagcag ccgcggattt caataccaaa tttctcggcg agatattttt
    58261 tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg ggcggaagaa cgtccaccgc
    58321 cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta atcagcatgg cctgggcgga
    58381 aaacgtcttt aattgcgctg taatcctgag agcgctggtc agtgttttcg atcaacaagc
    58441 caatgctggt gccagtagta acgccttcaa aaacaccgga gagaattttg acctgatccg
    58501 gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg acgacggtcg aggtcatgtt
    58561 gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc atcgacgatg cagccgagcg
    58621 ccagcccatg cgattcgccg aaggtggtta cgcgaaagag ttgtccaatt gtgtttccag
    58681 ccatcacggc tccgttattg ttgtgtttgc gtgtttactt aatctttata aatcgcgaaa
    58741 tattcacggg ccgccagcag ctgctctttg gtgagcataa acacaccatc gccgccgtta
    58801 tcaaactcca gccaggtgaa cggaacatcc ggatattgtt ccataagatg taccatgctg
    58861 ttgccgactt cacaaatcaa cacgccatca tcagcaaggt aatctgccgc gtttccaaga
    58921 atgcgacgcg tcagtttaag gccgtcggtg ccagatgcca ggcccagttc cggctcgtga
    58981 cggtattcgt ttggcaggtc ggacatatct tccgcgtcga catacggcgg gttagtgaca
    59041 atcagatcgt actgcacttt cggcaaatca cggaacagat cggaacgaat cggaatgacg
    59101 ttgtggatca gaccgtgttc ttcgatgttc tgttcggcaa ccgccagcgc gtcgggagag
    59161 atatctaccg cgtcgacttc cgcttccggg aaggcataag cacaggcaat ggcgatgcag
    59221 ccgctaccgg tacacatatc taaaatatgc tgcggttgct tgctaatgag tccggcaaat
    59281 ttattgttga tcagttcacc aatcggcgag cgcggcacca gcacgcgttc atcgacgtaa
    59341 aattcatgac cgcagaacca ggctttgttg gtcaggtaag ccaccggaat gcgctcgttg
    59401 acgcggcgga tcacgcgttc aacaatacgg tgtttttcgc tggaggtcag acgcgcggtg
    59461 cgcatatctt ccgggatatc cagcggcagg tagagcgatg gtaacaccag ctgtacggct
    59521 tcatcccacg ggttatcggt gccgtgaccg taccagatat ttgccgcgct gaagcggctt
    59581 accgaccagc gcaacatgtc ctgaatggtt tgcagctcat ttactgcttc atcaacgaaa
    59641 attttatcca cgtattcctc cagggcatga tcgcaataat ttcggcggct agtttgccat
    59701 gaagatgacg ataaatcagc attcacgcgc ggtgagtgag gaaaaatacg tttaaaacga
    59761 tcgattgtgc tacgagtcgg gtaaactgta ggaaaattag aaatagagac agataaatga
    59821 aaaagaaaac aacacttagc gaggaggacc aggctctgtt tcgccagttg atggcgggga
    59881 ctcgcaagat taagcaggac acgattgtcc accgaccgca gcgtaaaaaa atcagcgaag
    59941 tgccggtgaa acgcttgatc caggagcagg ctgatgccag ccattatttc tccgatgagt
    60001 ttcagccgtt attaaatacc gaaggtccgg tgaaatatgt tcgcccggat gtcagccatt
    60061 ttgaggcgaa gaaactgcgc cgcggcgatt attcaccgga attgtttttg gatttacacg
    60121 gtctgacgca gctgcaggcc aagcaggaac tgggcgcgtt gattgccgcc tgtcgccgcg
    60181 aacatgtgtt ttgtgcctgc gtgatgcatg ggcacgggaa gcatattttg aagcaacaaa
    60241 caccgctgtg gctggcacaa catccgcatg tgatggcatt tcaccaggca ccgaaagagt
    60301 acggcggtga tgcggctttg ttggtgttga ttgaagtgga agagtggttg ccgccggagt
    60361 tgccctgaat gctggcagca aatgccggag cagcgtaaac gccttatccg gcctacaaat
    60421 atgtgataat tcaataaatt gcaattcact tcgtaggcct gataagacgc atcagagcaa
    60481 tactacgaac tacagttcat tcaatacata ctgaaaacga cacgtcgaat caatcggttg
    60541 cccactactg gtattcacat tcaccacaat atcgtgtagt actttatccg ttaccggatg
    60601 acgagttggc gtaaccgtta ccgttgattt atcacaaccg taagaggtaa aggttaacaa
    60661 ttctgatacc gcgcggcagt atgctcctct gccatcatta tcagaaaaat tactgccatc
    60721 acagagtctg ccgccttcca tcaggtaggt cgaaagcgtg gtggtcactg ttgaccccgt
    60781 ggactgaatt cgtaacgcct tattatggct gacataattt tcactccagg agatatcgta
    60841 tgaagtgacc ggtatcgttg ggcatattgt gttatgcact gtaggggccg tatagcttcc
    60901 aaatccgggt aaatctgtat aggaataaaa ggatgcgatc caagggcaac cataatcatc
    60961 agatgcaacg gtaattttaa acgtcatata tgcacaacga aaaattttcg tatctatgcc
    61021 agggcaattt gagttattaa ttgtatcaaa gtcagtcagt accaaagttg gagctgaacc
    61081 cgacccagac caggaaataa cccgcaacgt atcgccttta tacgcaaaag aatcatattt
    61141 cccatatgta acctcctttg gcgttatcac cgtaacattg aaaacactgc tcgctacagc
    61201 accggttacc ttgtccatgg cataataaac tctcacactg tcaaaactcg cactcataag
    61261 tttatctcca gcataactct tagatgcgcc gacaacactc agaacgagca gcattaggct
    61321 cgcgaaaatt atggtccatt ttttcatttc agaaatactc aaaggttgcc gtcactgtgg
    61381 cactaaagtc tccggacgtt acatctcgcc cgctctttgc ctgtaaccag gtattaaaat
    61441 ttaggctgtt atttccctga ttcagcggga gcgtgacacc tgttgtattg ttgattggca
    61501 ccgatgtgcc tgccgcagtt tcaataccaa tcccagcgcc ggatgctgat gacgaggagt
    61561 caaaggccag aaagccgggt agagcactat cttctgttcc tgtcaacgtt actttgacat
    61621 taaacaatgt agaactatga caatctttca attgaaacac caatggcact cgctctgact
    61681 gcccaaggtt cattaaatcg ctggcggcaa tggcaggaaa atgtacttct gccaactggc
    61741 taccattaat cactggcgta cacgatttac tgattaaatt gccggtaaag tgcaaattat
    61801 ctcctgctat tgtggttcca ctgcccagca acatcaggct actaataaaa aacaacgtac
    61861 gctttttctt cattggtaat ctacctgtaa cgtggcccag gcctcaaagt ccgcctctgg
    61921 cagaacgaca ccactctttt ttactggcac agccgttaac accggtaaat cggtttcatt
    61981 caccataagt ggcgtattga tcgtaaaact ctgccctgcc tgtttgagct gaatgcctaa
    62041 accagtgata ttgctgctta ctgcagcatt atcaaaatcg cttagtgtgc cactccagct
    62101 caaggtcatt tccattaatg ggtcgcgaac cgccgtatcg caggtaatgg aatacggcac
    62161 ggtttgctca taattgctgc cattaatgtc atcaataagc acatcctgaa aagttatttc
    62221 aatggtttga gcgttgctaa tcgtgcagtt aggcggctgc actaaataac cgtgaaaaga
    62281 gacgtttgag agtgccatcg agcaaaagcc ccacagcaaa ataagcgaga tccgtttcat
    62341 tggtaatcca ccacaattgt ggcactggcg ttaaattcag ctgccgctaa cgttgtacca
    62401 ctctgtttca ccggcacagc ttccagaacg ggaactcccg aggaaaaatt aaatggcaac
    62461 catgagccag aggttaagtc gagtattgtg tgatcactac ttttctgaac ccgaatacca
    62521 aacccaggaa tacccgtgct gataactgtt tcaccgttaa tcacgaccgt tgtcgcctgc
    62581 atctgtagac gcaggtcatc cattgccaga ttgttacaaa caagcgagta gtcaatggga
    62641 cgtttataat caaccccatt gatcttatta atgaatacat taccaaactc aactgccgca
    62701 ccatttaccg tacaaggagg aggggcatcg acaagcaccc gtaaattcat acttactgac
    62761 ttactcgctg caactgcaaa gccgctacca acacaacata tcagtaaccc agttaccgcc
    62821 cagacagaac aagatccttt catactggct ctcccggcct cagcctttgc ggctctgctc
    62881 ttcatcgacc tggcaactgt taccgttgca agtgaaaaag agcggcatcc gagcgccata
    62941 gtcgttgaca taggtcagta ccggcacgct acccatcttc acattcagcg gtgcagaggt
    63001 tttcggcgac atcactattg gcgagaaacc ggctgcagga ttgccatttt tctgtgtgct
    63061 ggcattactg aaaatgacgt aatacggcgt cgggttattg acggtaaatt cctgaccgtt
    63121 gcgggttaaa gtaaccttat attgccaggg gtttttcatg ctgacgttct ccagcgcctt
    63181 cggtcgccag aacaacttaa tacgcgtttg tagtgctatt tgcagcgtgt tggctttgtt
    63241 tgattttggc gggatctcac gcacgttgaa gtagaacagg ctttcacgat cggcaggcaa
    63301 tttgttgata tccggcattc cctgcacttt tacctgcccg ttcatcattg aatcgatgcg
    63361 ttgcaccggt ggcagaacag ttaacggtga actgatcttg ttaccctttt catcttcgat
    63421 ccagctttgg gccagataag gcagtttcgg atcgttattg cgcaacgtca cgctgattga
    63481 tttgtcgctt tcattaaata tcaaacgggt acgatctggc gttacagaag cctgggcgac
    63541 aaactagcag cgctaagcag taacgctagc gtcgtagcac ccaatttggc agagcaaagt
    63601 aaatcagaca tggatcattc cttaatcacg ttattggtta gctgaaacag aaacaggggg
    63661 attggtgggt gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt catcaggaac
    63721 aaccggcgcg acgttgcctt tatgctggca tggcagtaac aagccgttaa acagatcggc
    63781 aggcagcggg tccggcaggt tgatatcgca atgcgcaaca ccactccaga acacactcat
    63841 gtgttcgcca ggtttcaccc cagccagata aacattgccg tcatcatcga caaggcccac
    63901 tgtctgctcg ttatcatttt ttacttctgc gccaaacgga ggatggctgc cgtcttgtaa
    63961 acgcagcacc gccatcgctt tttgaccact gatgacggca aatttgcggt agccaatcgc
    64021 cccttcagtt agcgtggctt gtaccaccga ctgggttgct tcggcgtttt ccggcagttt
    64081 gttgaggtcg atatacgcct gattgcggta atagttattg acgtcagaaa cgacggcttt
    64141 accaaacata ttggtataaa cagcagcacc gttaccttct accggaacat cggcaacgcc
    64201 atcggcatca atcaacaagc gtgtcccgcc catattctgg gtacggtgaa gtgcgccacc
    64261 gtgggcagta agcgttgcgc cgccctgtaa cgacaagccc gcagaggtgt attgcccttc
    64321 atggtagttc gcactgaggt caacctgcgc cagcgaacca tcatggctgt aatagccgtc
    64381 aacgctggtg tgtttgtcac tggtgccgac gttcaactga tagtgagtcg cgtcatcgac
    64441 acggctgaaa taaccgacct gactgctgtc cgtcccactg ccatagttac cgttatagct
    64501 aacggtactg ttgtcacccc acggcatact gagcgaaatg tacatgcctt tgtcggcctg
    64561 gttgtcatac tcgtaacggt agccagtcat cgagatgctg acattacgaa tactgcccat
    64621 attgaaatag tgcgagagca tgatgttgta gtttgtctgc tcctcgcgat cccagtaggt
    64681 atggcgggta tagttgagat aaaccgaaac acccgcatcg cggaagttct ggttataagt
    64741 ggcagtgtac atctctttgt cgttgcccgt gcgtaccatt ccgctgtcac tggcatccag
    64801 atactcgctc atggtcataa agttctcttc cgagaagcga tatccagcaa aagtaacgcg
    64861 gctgttgagc tggtcaaagt ctttggaata actcacacgg aaggagttac cgtccagcga
    64921 acctttgcca taagcggtgt ctttatccag tttggtgtgc gagtgagtaa catcaaacgc
    64981 gaccgcgccg aatgtagaca aatcgcgacc gacgccaagc gccgcagact gatagttttc
    65041 atctcccagt gcgccgccat ataacgacca gccgttagcg atcccccagg aagcttcagc
    65101 accagaaaaa aattcaccct cgacatggtg tccccactct tgcggacggc ccatcatgat
    65161 cttatagcga acctgacctg gacgagtgag gtatggcatc gaggcggtgc tgatgtcata
    65221 ttcctgcacc tggccgttct gttcttcaat gcgaatatgc aacgtaccgg agacagaatc
    65281 accaagatcc tgaatacgaa acggtccagc cggaacctgc gtttcgtaaa tcacacgccc
    65341 catctggctg acggtcactt ttgctgtggt gtgtgccacg ccggaaatgt ctggcgcgta
    65401 gccgcgcaga ttgggaggca acatttgatc gtcagtactg acactgccac caacatagtt
    65461 aaaaccatca aaaatatcgg atctgaggta atcctcgccc agcgccagtt tggcttttaa
    65521 tgatggtaac gcccgctagg catagtagcg actccactcc catttttttt gagtttcatc
    65581 gccgctgaat tcatcgtcat cattactgcg agtatgttga tagttagtct gccagtcagc
    65641 acgcatacgc cacggcccca ggttaacccc gaccgtcccg ttgccgctga tctcgttact
    65701 atcatcaccg ccattttctt cgtgccgggt ttgtgcgttg atgctgtagt ccgcgacgat
    65761 cccggagatg ccgtcatccc aacgtgaagg cggatcccag tcgggataag tatattcgag
    65821 gtaagcctgt ggcagtgaaa tgactaatgc ggactggctt aaatcagcct taatttccat
    65881 gccttccagt tgaccggatt tcaggcattt agcatcgtgg ctccattgca gatttttcgc
    65941 cacgtcttct tttaaaccaa actgcgctac cagttccggt gtcagacaag catagctttt
    66001 gctcgcgtca tcttcaccag cataccagta aatatcgtac tcttccgcca atggctgttt
    66061 atttagttga acctgtaaat tgtattttcc gggctcaaca tatccctggc tggaaaaacg
    66121 cttcagatca atttttgtgt cgccttttaa ttccagaaaa cgggaatcaa actgaatgtc
    66181 gtcttcagcc cagacactgc tataactccc tgacattgcc agcgcaatgc accacggaag
    66241 aatccgtaat cgaaaaagag aatggtcagg catacacgtt tctatccgtt tttattcttg
    66301 ttcggtcaaa atccatttaa atcccccata gcagtgtcgc tatgggggta tgccttacag
    66361 gtaagtaatc tggaaggtgg tatttgcttc gaaattaccc agatctggcg catcagctgc
    66421 gccgaccagc caggctttaa agttcagtgt ctgcttcgct ttgcctttat tggtagaagt
    66481 gtctttcact attttctggt caatacccat gccgcttttg taagaggtac ccagagagtc
    66541 accgatcgcc aggctaacat tgttaaatgc cgcacaggta tcggtattga aaatggtgta
    66601 ataattgccg ctatttgcag aagaaacggt accagtaaag gtagtggtca ttgtttcctg
    66661 agtatcaaat acgcaatctt gcagacgaat ctggaaactt ttcggcgtgg tggtgccgtt
    66721 atttttcagt ttttcggcac cgatatcacc catatcaact tccagtttgt gatcgtccgg
    66781 aacgatagaa cacactgagt cttcaatggt gccgtaaaaa cgtgcagtac cgttgttacc
    66841 cgcagcgatt gttgccgtag aggtgaacac gcccatcacc attgccgcag caatagctgt
    66901 tttaacaaac ttactcatta tttgtcctta aacta
//
LOCUS       DAZOZY010000003        60957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZOZY010000003 DAZOZY010000000
VERSION     DAZOZY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.003"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHURQ_000133"
     CDS             <1..468
                     /locus_tag="ACHURQ_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822018.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHURQ_000134"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHURQ_000134"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR0822019.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHURQ_000135"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHURQ_000135"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR0822020.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHURQ_000136"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHURQ_000136"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR0822021.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHURQ_000137"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHURQ_000137"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR0822022.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHURQ_000138"
     CDS             complement(4084..5313)
                     /locus_tag="ACHURQ_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR0822023.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHURQ_000139"
     CDS             complement(5377..5817)
                     /locus_tag="ACHURQ_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR0822024.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHURQ_000140"
     CDS             complement(5922..6692)
                     /locus_tag="ACHURQ_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0822025.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHURQ_000141"
     CDS             complement(6689..7615)
                     /locus_tag="ACHURQ_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0822026.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHURQ_000142"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHURQ_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR0822027.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHURQ_000143"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHURQ_000143"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR0822028.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHURQ_000144"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHURQ_000144"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR0822029.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHURQ_000145"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHURQ_000145"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR0822030.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHURQ_000146"
     CDS             13322..13669
                     /locus_tag="ACHURQ_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR0822031.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHURQ_000147"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHURQ_000147"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR0822032.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHURQ_000148"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHURQ_000148"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR0822033.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHURQ_000149"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHURQ_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR0822034.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHURQ_000150"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHURQ_000150"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR0822035.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="ACHURQ_000151"
     CDS             18978..20735
                     /locus_tag="ACHURQ_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR0822036.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHURQ_000152"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="ACHURQ_000152"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR0822037.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHURQ_000153"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="ACHURQ_000153"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR0822038.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHURQ_000154"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="ACHURQ_000154"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR0822039.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHURQ_000155"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="ACHURQ_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR0822040.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="ACHURQ_000156"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="ACHURQ_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR0822041.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHURQ_000157"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="ACHURQ_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR0822042.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHURQ_000158"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="ACHURQ_000158"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR0822043.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="ACHURQ_000159"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="ACHURQ_000159"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR0822044.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHURQ_000160"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="ACHURQ_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR0822045.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34428
                     /gene="gspE"
                     /locus_tag="ACHURQ_000161"
     CDS             33043..34428
                     /gene="gspE"
                     /locus_tag="ACHURQ_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR0822046.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            34418..35620
                     /gene="hofC"
                     /locus_tag="ACHURQ_000162"
     CDS             34418..35620
                     /gene="hofC"
                     /locus_tag="ACHURQ_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR0822047.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHURQ_000163"
     CDS             complement(35655..36698)
                     /gene="guaC"
                     /locus_tag="ACHURQ_000163"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR0822048.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36854..36898)
                     /locus_tag="ACHURQ_000164"
     CDS             complement(36854..36898)
                     /locus_tag="ACHURQ_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822049.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36923..37543
                     /gene="coaE"
                     /locus_tag="ACHURQ_000165"
     CDS             36923..37543
                     /gene="coaE"
                     /locus_tag="ACHURQ_000165"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR0822050.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37543..38286
                     /gene="zapD"
                     /locus_tag="ACHURQ_000166"
     CDS             37543..38286
                     /gene="zapD"
                     /locus_tag="ACHURQ_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR0822051.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38296..38493
                     /gene="yacG"
                     /locus_tag="ACHURQ_000167"
     CDS             38296..38493
                     /gene="yacG"
                     /locus_tag="ACHURQ_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR0822052.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHURQ_000168"
     CDS             complement(38593..38982)
                     /gene="mutT"
                     /locus_tag="ACHURQ_000168"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR0822053.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHURQ_000169"
     CDS             complement(39042..41747)
                     /gene="secA"
                     /locus_tag="ACHURQ_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR0822054.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHURQ_000170"
     CDS             complement(41809..42321)
                     /gene="secM"
                     /locus_tag="ACHURQ_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR0822055.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHURQ_000171"
     CDS             complement(42552..43469)
                     /gene="lpxC"
                     /locus_tag="ACHURQ_000171"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR0822056.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHURQ_000172"
     CDS             complement(43570..44721)
                     /gene="ftsZ"
                     /locus_tag="ACHURQ_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR0822057.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHURQ_000173"
     CDS             complement(44782..46044)
                     /gene="ftsA"
                     /locus_tag="ACHURQ_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR0822058.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHURQ_000174"
     CDS             complement(46041..46871)
                     /gene="ftsQ"
                     /locus_tag="ACHURQ_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR0822059.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHURQ_000175"
     CDS             complement(46873..47793)
                     /gene="ddlB"
                     /locus_tag="ACHURQ_000175"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0822060.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHURQ_000176"
     CDS             complement(47786..49261)
                     /gene="murC"
                     /locus_tag="ACHURQ_000176"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR0822061.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHURQ_000177"
     CDS             complement(49315..50382)
                     /gene="murG"
                     /locus_tag="ACHURQ_000177"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR0822062.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHURQ_000178"
     CDS             complement(50379..51623)
                     /gene="ftsW"
                     /locus_tag="ACHURQ_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR0822063.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHURQ_000179"
     CDS             complement(51623..52939)
                     /gene="murD"
                     /locus_tag="ACHURQ_000179"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR0822064.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHURQ_000180"
     CDS             complement(52942..54024)
                     /gene="mraY"
                     /locus_tag="ACHURQ_000180"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR0822065.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHURQ_000181"
     CDS             complement(54018..55376)
                     /gene="murF"
                     /locus_tag="ACHURQ_000181"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR0822066.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHURQ_000182"
     CDS             complement(55373..56866)
                     /gene="murE"
                     /locus_tag="ACHURQ_000182"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR0822067.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHURQ_000183"
     CDS             complement(56853..58619)
                     /gene="ftsI"
                     /locus_tag="ACHURQ_000183"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR0822068.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHURQ_000184"
     CDS             complement(58635..59000)
                     /gene="ftsL"
                     /locus_tag="ACHURQ_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR0822069.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHURQ_000185"
     CDS             complement(58997..59938)
                     /gene="rsmH"
                     /locus_tag="ACHURQ_000185"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR0822070.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHURQ_000186"
     CDS             complement(59940..60398)
                     /gene="mraZ"
                     /locus_tag="ACHURQ_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR0822071.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14596 a        16851 c        15001 g        14509 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgattttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacta gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaagc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgcgatggc gaaacggcag aaattgccat taaagccgcg
    33961 caaaccggtc acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta
    34021 cgtttacagc aaatgggagt cgcccgctgg atgctctcat cagcgcttac gctggtaata
    34081 gcccagcgtc tggtacgtaa actttgccca cattgtcgcc agcagcaagg ggagcccatc
    34141 catattccag tcaatgtatg gccgtcgccg ctgccccact ggcaggcacc cggttgtgta
    34201 cattgctacc acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg
    34261 gtcattcgtc agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga
    34321 caggcgggta tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc
    34381 acctttgaag agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg
    34441 ctggcatggc ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg
    34501 tgctttgctg ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat
    34561 cgccatcaat tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc
    34621 gacgctactc aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca
    34681 tcccagtaag caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat
    34741 tgctttttcc aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat
    34801 gatccgcacg ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca
    34861 gcaaaaagcc cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat
    34921 cattttagcg atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt
    34981 tgccgctatc tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac
    35041 gctggcagac tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc
    35101 gatagccaat aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct
    35161 gttacgcatc ccgattatgg gttcactgat gcggggacaa aaactcacgc agatctttac
    35221 gattctggcg ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag
    35281 agaaacaatg cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag
    35341 taacggtcat cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt
    35401 gcaattagtg agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc
    35461 ccatcatcat cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc
    35521 gacgttgctg atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc
    35581 aattttccat ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca
    35641 ccagcgttgg aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata
    35701 aatgtggtgc gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa
    35761 cgcaggccgc ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta
    35821 acggttttac cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca
    35881 gactcggagc tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga
    35941 ccgccgctct cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg
    36001 ccaaaggctt ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg
    36061 cccagaccgt gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg
    36121 cctgttttga cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca
    36181 gcaccggaga ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg
    36241 gttttggtcg gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa
    36301 ccattcgcca catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta
    36361 gttttttcga aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca
    36421 gcggaagaat tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca
    36481 gtcaaaatat caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata
    36541 tttgcggcga taatcggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga
    36601 cgttccagtt caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg
    36661 tctttaaaac ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg
    36721 gctataaggg taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc
    36781 aagactgcga aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg
    36841 agcgaacgtt tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga
    36901 tgaattcccg ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag
    36961 tggcaagagt accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga
    37021 tattattgcg cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca
    37081 ctttggcgct aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg
    37141 gatcttcgcc aacccggaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca
    37201 gcaagagacg caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc
    37261 attgctggta gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag
    37321 cccagaaacg caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga
    37381 acaaatcctt gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat
    37441 tgataataac ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta
    37501 tttgcagctt gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt
    37561 tttgaacatc cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag
    37621 caactcaccg ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat
    37681 gtcagtgaat tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa
    37741 cttgaccggc agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc
    37801 cgtattgaag cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg
    37861 cgtatcgggc aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc
    37921 atcccaggcg gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc
    37981 caggcgcagc gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag
    38041 gcacttacca tggtgctgga tttaattcgc cagtcggccc ccttccgtaa acaaaccagt
    38101 ctgaatggtt tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg
    38161 ctcgattcac agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt
    38221 atgccgctgg acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt
    38281 tgctaaggag taaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg
    38341 gtggtgtggg gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc
    38401 gacctcggag aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa
    38461 agcgatgact ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtaggcct
    38521 gataagacgc ggacagcgtc gcatcaggca tcttgtgcaa atgtcggatg cggcgtgaac
    38581 gccttatccg acctacagac gcttaagctt cgcaattacc ggttcattgg ctggcggaaa
    38641 atcatcggca ttaagaccga ctagcgacat ccactcaccg ggttgccctt ctttacccca
    38701 cggctcccct tcccagcttt cgaccagcca gaaccacaga gttatatgcc tgtccgggaa
    38761 ttcatattcc agtttttcaa atagcgaaaa atgttgtggg gtaatcccga cttcttcctg
    38821 aagttcacgc actaccgcct gttccggcgt ttcgcccatt tcaattttac cgccgggaaa
    38881 ctccagttta ttcgccatgt gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt
    38941 ctcgttgcga ataataccta ccgcaatttg cagctttttc attgtcttaa acctataaaa
    39001 aaggcgcaga atcctgcgcc ttttacttca actgttagct tttattgcag gcgaccatgg
    39061 cactgcttgt attttttacc agaaccgcac gggcaaggat cgttacgtcc tactttgcgc
    39121 tcaccggttt gcgccgccag tgcagctgcg gctgcagagt cgtcatcctg atggctaagc
    39181 tgctgcattt gcgctaaacg ctcggcttcc atacgacgct gttgttccag ctcctcaacc
    39241 tcttcaggca tacgtacctg gactttgctc agcgtgctga taacttcata ttttaacgac
    39301 tccagcatcg ctgcaaacat ggagaacgat tcacgtttgt attcctgctt cggatctttc
    39361 tgtgcatagc cacgcaggtg gataccctga cgcagatagt ccatcgccgc taggtgctct
    39421 ttccacagag agtcgagagt ttgcagcatg acgccttttt cgaagtgacg catcatctca
    39481 gcaccaacca cttcttcttt acgctgatac acttcgatgg actgcgccag aattcgctca
    39541 cgcagcgtct cttcatgcag ttctggttct ttatccagcc actcggcaat tggcaaatcg
    39601 aggtcgaaat cgttcttcag acgttcctgc agccccggaa tatcccacat ttcttccagt
    39661 gactgtggcg gaatgtaggc atcaatggtc gctttgaaca catcttcacg aatgctgttg
    39721 atggtttcgc tcacatcgct gacatccagc agttcgttac gctgggagta aatggcgcga
    39781 cgctgatcgt tagccacgtc atcatattcc agcagttgct tacgaatgtc gaagttacgg
    39841 ctttcaactt tacgctgggc gttggcaatc gctttagtca cccacgggtg ttcaatggct
    39901 tcgcctggct tcatacccag tttacgcatc atgccggata ctcggtcgga agcaaaaata
    39961 cgcatcagcg catcttccat cgacaggtag aaacgggaag aaccagcatc cccctgacga
    40021 ccagaacgac cgcgcaactg gttatcgata cgacgggatt cgtgacgctc ggtaccgatg
    40081 atatgcaggc cacctgcttc cagtaccgca tcgtgacgta cctgccagtc ggctttaatt
    40141 ttttcaattt gctctgcggt cggattttcc agcgcggcaa cttctgcctg ccagctacca
    40201 ccgagcacaa tatctgtacc acgacccgcc atattggtcg cgatagtcac cgcagccgga
    40261 taacctgcct gagcaacaat cgccgcttcg ttggcgtgga atttggcgtt caggacgttg
    40321 tgcttaatac cggctttggt cagttcgttt gacaccagct ccgatttttc gatggagata
    40381 gtacccacca gcaccggctg gcctttcgca gtacgttctt tgatatcttc aatgatcgcc
    40441 tgaatttttt ccgcttcagt catgtagacc aggtccggca gatctttacg aatcattgga
    40501 cggttggtcg gaacaacgac ggtatccagc ttgtagatgg agctaaattc gaaagcttcg
    40561 gtatcagcag taccggtcat ccccgccagt ttttcataca gacggaagta gttctggaag
    40621 gtgatcgaag ccagcgtttg gttttcgttc tggatctgca caccttcttt cgcttccaca
    40681 gcctggtgca gaccatcgga ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca
    40741 acgatgataa cttcaccatc tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga
    40801 gcgcgcagcg ccgccgttac gtggtgcatc agcatgatgt tggccggaga gtacagagac
    40861 tccccttcat ccatgatgcc ctctttcacc agcagttctt caatcagcac cagaccacgt
    40921 tcggtcaggt tcacctggcg agatttttcg tccaccgaga agtggccttc gccctggaag
    40981 gtttcggagt cttctttttc ctgacggatc aggtgcggaa taattttatt cacgcgttta
    41041 tacatttccg agctgtcttc tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg
    41101 atcaggatgg agtccacttc gtccaccagc gcatagtgca gtttacgctg tacacgttct
    41161 tcagggctga acgccatatt gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg
    41221 taagtgatgt cagctgcgta agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg
    41281 ataccgacag tcaggccaag gaattcaaac agcggacggt tgttttcggc gtcacgttgc
    41341 gccaggtagt cgttgacggt aactacgtgc acgcctttac cggttagtgc gttcaggtaa
    41401 gcaggcagcg ttgcggtcag ggttttacct tcaccggtac gcatttcggc gatgcagcgt
    41461 tcgttaagaa ccataccgcc gagtaactga acgtcgaagt gacgcatacc aaagacgcgc
    41521 ttacttgcct cacgtaccac ggcgaaagct tccgggatca gattttccag cacttcgcct
    41581 ttttccagac gtgcacgaaa ctctgcggtt ttccctttca gttcttcgtc ggagagtttt
    41641 tccatctccg gttccatggc attgatgatg ttgaccactt tgcgcatccg gcgcagggtg
    41701 cgatcgttac gactaccgaa aactttagtt aacaatttga ttagcataat aaaatctcaa
    41761 acgccccgcg ttgcggagtt aataaaatga agtaaaggtt tattgttgtt aggtgaggcg
    41821 ttgagggcca gcacggatgc cttgcgcctg gcttatccag acgggcgtgc tgaattttgc
    41881 ttgtggggta aaatgcgcat aatcaatgcg ataacccttt tcagacggcg tgccttcctg
    41941 ggtcagcagc gcgctgagcg tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga
    42001 ttcttcagca acgggcagtg tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt
    42061 gcgaatggca tgttgatgcc agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt
    42121 cgcttccagc aaggccaatt gaccaaagtt aactttggct gaaggctcgt ggttgcgggt
    42181 tgtcgctttt gcgggcgcgt ttggttcggc ggcgttgctg agcgcaggca aacctaaact
    42241 cgccgcaacc atccctaata agagatgcgg ccagaagtag cgtttaccaa actgtcgcca
    42301 gcgcgtcagt attccactca cgttattgcc atcccgatcg tattcaaacg tatttagcgc
    42361 acaaatctta tcattaaagc ctgcagtcca cagcacgaat gaagataagt tgtgcggaaa
    42421 agtgcttaag aaaacagcgt tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa
    42481 atgactggtt cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag
    42541 tataaatgtc gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg
    42601 tcgtcctgga aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt
    42661 ttgttattca gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca
    42721 cacatgaaca agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa
    42781 cgcaggccgt cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg
    42841 ccgcccaggc acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta
    42901 cgcgcacggc tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg
    42961 ttgctggaat caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg
    43021 tacggcttaa attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga
    43081 acaaattttt tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac
    43141 ggagcggcgc tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta
    43201 tcgatgccca ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca
    43261 tgctcgttga ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg
    43321 aaatctaccg gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca
    43381 gggcgtaacg tcagagtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg
    43441 atacgtttaa gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc
    43501 gaagtgtact atacatccgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc
    43561 agtcaattct taatcagctt gcttacgcag gaatgctggg atatccagat aatccggctc
    43621 tttcgcagtt tgcggcgcat tgtcattcac gactttagca accggcttct gctcctgggt
    43681 cagcggagcc atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt
    43741 ggtcaccaga gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac
    43801 gcgcagctcg tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc
    43861 ggaagcaaat gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa
    43921 gcccgccgtg atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag
    43981 cggagaagag atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc
    44041 agaacccatc attgcgtagc ccatctcaga cattacggtg cgtacgtctg caaagtccac
    44101 gttcatcaaa cccggacgag taatcagttc agcgatacct tgcacagcgc ctttcagtac
    44161 atcgttcgct gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag
    44221 tttgtcgttc gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg
    44281 ctccgcgaat gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac
    44341 ggtcaggata cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt
    44401 accaccaccc atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa
    44461 tgcatcgcga tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag
    44521 tcctttggtg ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc
    44581 ttgtgcatcg gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg
    44641 ttcaacagca ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt
    44701 aagttccatt ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa
    44761 tcatcgtcgg cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat
    44821 ccacgagcca actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga
    44881 ctctttccca tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc
    44941 cgttaaaccg gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg
    45001 agcacaggct gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc
    45061 caggtgatgt ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt
    45121 gaccaggttg agcagctcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg
    45181 cagactccgt ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac
    45241 gatggaaccc agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt
    45301 gccaaaggcg taagcgatat cactggtcac gacattgcca gcataaggaa ttaccttagt
    45361 gtggcgcaat gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac
    45421 gacgcagaca cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc
    45481 ggcaaatatc agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt
    45541 cgccatatcg ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag
    45601 tcctaccgga ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg
    45661 cagcacacga tgttcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac
    45721 atcttcttgc gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct
    45781 gatgtgctta ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc
    45841 tgcctggtca atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc
    45901 tttatccata ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg
    45961 cagaacttcc cctactaaag cggcaacctt cgcggtacca atctccagtc ctactaccag
    46021 ttttctgtcc gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta
    46081 gattcctctg gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca
    46141 acgtagctaa tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct
    46201 acaaagcgag ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta
    46261 ttcagcgtca actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg
    46321 tcctttgcca gcatctgccc catttcgcga tagccctgca acacttcatt ggcgctgcct
    46381 tccgggccat acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag
    46441 gtgtttcctt ccgcgtctac catatgttga tcattccacc gcgcaatcgg cacatattca
    46501 accagatgaa tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac
    46561 ggcaggcgtt gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc
    46621 ggctcaccca atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca
    46681 ccggtcaaca ccagctttga gagcggcagg cgttgcgcat cttccatcca gcccaacacg
    46741 acccagccgc tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga
    46801 cgcgttccat tattgcggcg agaagaaacc tcttcttcgc tatttcgcgt gttcagagca
    46861 gcctgcgaca tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat
    46921 acctgcctga cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt
    46981 ggcttccagc agataaaact gtccatcgct gtccagcata acgtcaatac gtccccatcc
    47041 tttgcaacct aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga
    47101 cgcttccaga cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata
    47161 atcatagaag gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat
    47221 cgcaaccgtg aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg
    47281 aaatgccaat cttaatgcat cttgtagagc attttctgct actacttttg acattcccac
    47341 actggaacct tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc
    47401 taactgctta tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc
    47461 gaccggtaaa ccggcacctt gccacagcag tttgctgcgt agtttatcca ttgaaagcgc
    47521 agatgccatc actccgctgc cggtataagg caagcccatc agctcaagca tgccctgcag
    47581 cgtaccatct tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga
    47641 cttcagttgc gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc
    47701 acgcagcccg gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt
    47761 cccgcccaac aggaccgcaa ttttatcagt catgttgttc ttcctccgga gtttgcggct
    47821 tcagtttgat ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga
    47881 gaatcaggtc gttaccgatt aataccggtg ccagcatctc ggctacctgc gcagggtccg
    47941 gcaccagaat gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt
    48001 ccgctcccgg aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa
    48061 cctgcgtcag cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg
    48121 gctgaaacag cattactagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg
    48181 cgtcaacttc cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac
    48241 cattcactgg ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga
    48301 agctctccag cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa
    48361 ccgcagctgc ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcgcatcg
    48421 gctctttgtc ctggcgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt
    48481 ctacacgcac gtcggcatct tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg
    48541 gtaacaattc gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaacggca
    48601 ggttgtgcag aaaattaata aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat
    48661 ccatgtggtc ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg
    48721 atgcatcact ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa
    48781 cccccgccgc ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt
    48841 agatgctgga aaccatcgcg gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc
    48901 catgacgaaa acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg
    48961 cttcatgagc tgcgacaatt tccgggttat cggcagaaat ggcgctggaa acaacgacca
    49021 cgctggcatc acgtacgttt tccgggcgat ggttgaaata aatcgtcgct cccagattca
    49081 ttaactgctg cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat
    49141 tggccagaac ttcggcaata ccgcccatac cggcaccacc gatgccgaca aaatgtatgt
    49201 gccgaacgcg acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca
    49261 ttctttacgc cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc
    49321 ccgggcagcc cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc
    49381 gcgggcgcgt tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc
    49441 gacagcatcc acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag
    49501 cggtagcgca ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg
    49561 tagtcctgcc gcggcgattt cactcaccgt taaagcaccg gagcggcaaa cgacgacatc
    49621 cgcccacgca tacgccgccg ccatatcatc aataaattcc gtcactttat gctgcggttg
    49681 ccccgcttcg gcatacgcct gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca
    49741 gatagtgact gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg
    49801 tgcgccctgg gaaccaccca ctaccagcac acgaaccgga ccttcacgtc cagccaaacg
    49861 ttgctgcggc aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc
    49921 attagggaaa gcgcctggaa acgcctgcat cactttggtg gcaatcttcg ccagccattt
    49981 attggttaag cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca
    50041 cgcggccaga ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt
    50101 gtacgctttc ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc
    50161 tatcagtgct tttatacctt ttccacgcag accagagata cgaatgaaat caatttcgat
    50221 gccatgtttt ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg
    50281 ccaaccctga gccattagat ggtgcgcaac cgccagtccc gggaatacat gtccaccggt
    50341 tccgcctgcc atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac
    50401 gcctgcgctt tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc
    50461 gacataatca gtaagctcga accaccgtaa ctgatcagcg gcaatgtcag acctttggtc
    50521 ggtaacatcc ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca
    50581 atagaacagg cgagaaaacc ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc
    50641 gacatcgcgc gaaaagcgac gaagaatacc attaaaagtg ccagcaccac accgacatac
    50701 cccagttctt cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc
    50761 agtttttgta ccgagttacc taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc
    50821 agcgattgcg ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg
    50881 gtaacacggc ggatacggta cggttcggcg agtatcagca acacaaccgc tgaaatgccc
    50941 ataccgataa tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc
    51001 gtagtcacaa acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc
    51061 aacaccagaa tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg
    51121 cctttacgca ccagatagtt ggcgatataa caaaacagcg acagttttgt cagctccgca
    51181 ggctggatac gcagcaaacc gagatcgatc caacgcgatg cccctttaac cgagctaccc
    51241 actaccagca cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt
    51301 tgccagaact ccatcggcag acgcagcgta atgatcgcca gaatgaatgc caggatcaga
    51361 tagacgccat cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc
    51421 attgacgccg aggtcaccat gataaagcca atcgccgcaa ggccgaaggt cagccacagt
    51481 aaggtgcgat cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag
    51541 ccttttagcg ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc
    51601 aggcgaggga gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc
    51661 ctcgttgttc aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca
    51721 tatcgcccag ctgaacacgc ggagccagca agcgcatcgc ttgttccatg gtttcggttt
    51781 gttctgccac ttccgggcgt agcgccgcca gctgcgcacc gtcacgacca aaacagtaca
    51841 aacgcacgtt gtcgccattc agatagcgcg ccagtggact gaagtccgcc gatttaccat
    51901 cgccgcccag caacaaatgc agcgtgccgt ctacctgcaa gccattcaac gccgcttccg
    51961 tactgccgac gttagtggct ttcgaatcgt taatccagcg tacgccattg tgctccagca
    52021 ccacttcaaa gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc
    52081 gcggcaaacc agctgcgtct gccagtgcca gtgctgccag cgcattggtg tagttatgct
    52141 gcccggaaag tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg
    52201 tttcgccctg ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc
    52261 gttcatcagc accgcgaatc ggcattgtta aggcatcatc agcattgacg acacaaactt
    52321 tcgcattttc gtaaatacgc aattttgccg cacggtattg ctgcaaacca aacggatagc
    52381 ggtccatatg atcttcagtc acgttcagaa tggtcgccgc caccgcctgt aagctggaag
    52441 tggtttccag ctggaagctc gacagttcca gcacgtacag ttcgcactca gcatcaagca
    52501 gcatcaacgc tggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg
    52561 ccatttcacc cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa
    52621 tcggtgcttg tgcttcgcga cagaacagct cgatatcgcc aacgatttcg attccggcat
    52681 cagcggcagc gcttaaggat ggatgcgcct gtgcaatacc gggactggcg acaatcagat
    52741 ctgccgccat cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta
    52801 atttatccag gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag
    52861 cgaggaaaaa gtccacgcag gaaagcccgg tgaggcccag gccgataatg acgacatttt
    52921 taccctgata atcagccatg attaacgtac cttcagcgtt gccagaccaa tcagaaccag
    52981 catcagcgaa ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc
    53041 atagtggtga tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc
    53101 gacctgcagg atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag
    53161 gaattcctga cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc
    53221 tacatcgccc ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc
    53281 cccgactatc gccgtgcaga caatgaccag ttcccctgcg tgtcgcagat acggtatatg
    53341 caagtagctg gcaaagttca tattgccggt cgcccacgcc accagcgcaa aaccaccggc
    53401 gacaaatacg gtcggcataa ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt
    53461 gccagtaccc acaatgacga agtaagccag cagaatgtag aacagcccca gctgcggcat
    53521 cacatcttta aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta
    53581 cagggcgaag gcgacaccca gcgcaatgac cgacatccag aaatacttcc aacgagcgat
    53641 caaccctttg gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc
    53701 gtaacctacc agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag
    53761 cagtacggag atcacaatcg ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg
    53821 cttgctgaag tgtgattcag gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg
    53881 caaatgagct atcatacgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct
    53941 gacgatggcg cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa
    54001 atgttcggcc agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc
    54061 atggcggcac tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt
    54121 gatttaagac gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg
    54181 gtgctgatag catggctttg tttacccacg cttaacacgc ggtcaatacc agcagctttt
    54241 gccgcctcgc ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg
    54301 cccaccacca gcacgcggta gcccggcatt tcagccagta cctgaacagc ggcggtcatt
    54361 gaaccgacat tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg
    54421 gggaacagac ggcctggaac agctttcaga tttgccagcc ccgctttgat agcattaagc
    54481 gttgcgccca cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc
    54541 cccggcaacg gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccgta
    54601 ccgtgcgagg tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag
    54661 aaacgccaca ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg
    54721 gcgttcataa tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc
    54781 gcgacacccg caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc
    54841 gcagcttccg ggcgagtcag actcacagtc caggctattt cgccctgatg gttcgcgcca
    54901 agttcaataa ctgcgtaatc gtattccggc gttaagcgca acaacgtcat cggtacaccg
    54961 atgtcgttgt tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc
    55021 gccgtcatct ctttaacgga ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc
    55081 ggaacttgct ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg
    55141 attaactgcg gcaggtcgat atccagcgga cggctgacca gtagtgcgcc cgcgccgcca
    55201 gctttcgcct ggtcggcaaa atcatgagca tcgaaacgtt cgcctttcag ggcaacaaac
    55261 aggcagcccg gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca
    55321 ccttgcagtt caccgttgag aatgtcggta agttggctaa gggttacgct aatcatgcaa
    55381 tcacccccag cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa
    55441 caatctggta atcttcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct
    55501 gcataacggc gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat
    55561 ctaacattcc cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat
    55621 cgtccgtcac caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct
    55681 tacctttatc gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca
    55741 ggcgcgccgc ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca
    55801 ccgtcggttt gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg
    55861 cggcggtttt cagcagatca gccagtggat agccgagtgc cagcagtgtc gccagcgcga
    55921 gcagcaggtt gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc
    55981 aacttgagct aaagcgaatc gtcgcaccgc tgtcgtgata gttcacgtcg atcgctttta
    56041 accagcgtcc gtgacagttc ggattaatat gatcttccat tgataccgca accgcgtccg
    56101 gcagttttgc cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc
    56161 aatgatgttc agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat
    56221 aatcaaggtg atcgcggctt aagttggtaa agaccgacgc cgcaaatttc aatgccgcca
    56281 cacggtgctg taccagcccg tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct
    56341 ccaccagccc cgccagctca tgctgaacat cgactgcgga accggtagta ttttctgtcg
    56401 ggatcacttt cccaagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa
    56461 gcagttggct ccactgcgcc agcagctggg tagtcgtggt tttgccgttg gtgcccgtta
    56521 cgcctacgag acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata
    56581 aacgctcatt gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac
    56641 cgtcggtcgc ctcatctttc gctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    56701 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    56761 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    56821 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    56881 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    56941 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    57001 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    57061 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    57121 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc cttcacgccg
    57181 ccgccgcctg gtagcgccac gctttccatc atatgcacca cagtgcggac aatggatccc
    57241 gggaagacac gttcaccggg aaccgggggg tcaactttgg taatcgacag tgggcgataa
    57301 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    57361 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    57421 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    57481 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    57541 ctcgacttct gtaatacccc ggtcagggtt aattcgctgt agcgtgccac gtctttgatt
    57601 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    57661 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    57721 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    57781 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    57841 ccagattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    57901 aggcgttcat caatactcag cgccaggttg tgcgctgcct ggctgtcagt agaagaaata
    57961 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    58021 agccatttat cgaaactctt ctcaacgccc tcaatccctt gactatcgac gttagtaaag
    58081 ccgatgaggt gagcagtcac ttcgccggac ggatagtaac ggcgagactc ttcacgcaga
    58141 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt cacctgacgc
    58201 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggctgaaag ctgatccagc
    58261 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    58321 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    58381 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    58441 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    58501 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaactg
    58561 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    58621 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    58681 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    58741 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    58801 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacagtcac cgccgtcaaa
    58861 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    58921 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    58981 tctgtcactc tgctgatcat gcattcgtcc tctctgcaat acgcagaact gaactacggg
    59041 cacgagggtt ctcagccacc tcttcttcgc ccggcattaa cttgcctagt gctcgcagct
    59101 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    59161 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    59221 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    59281 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgggtcgcgg
    59341 gatgtttaaa cttatctttc accggcgttg caacagccac gacttccgcc agttctttgg
    59401 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    59461 aacgctcttc accataggtt ttcaataccc aagcgatatc ggcttcttct gcggtttgta
    59521 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    59581 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    59641 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    59701 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    59761 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    59821 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    59881 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    59941 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    60001 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    60061 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    60121 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    60181 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    60241 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    60301 tgaccgacag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    60361 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    60421 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    60481 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    60541 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    60601 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    60661 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    60721 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    60781 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    60841 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    60901 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacg
//
LOCUS       DAZOZY010000004        57428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZOZY010000004 DAZOZY010000000
VERSION     DAZOZY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.004"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            145..1488
                     /gene="argG"
                     /locus_tag="ACHURQ_000187"
     CDS             145..1488
                     /gene="argG"
                     /locus_tag="ACHURQ_000187"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR0822072.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1496..3121)
                     /locus_tag="ACHURQ_000188"
     CDS             complement(1496..3121)
                     /locus_tag="ACHURQ_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0822073.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3580..3666)
                     /locus_tag="ACHURQ_000189"
     tRNA            complement(3580..3666)
                     /locus_tag="ACHURQ_000189"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3630..3632),aa:Leu,seq:gag)
     gene            complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHURQ_000190"
     CDS             complement(3681..4013)
                     /gene="secG"
                     /locus_tag="ACHURQ_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR0822074.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHURQ_000191"
     CDS             complement(4241..5578)
                     /gene="glmM"
                     /locus_tag="ACHURQ_000191"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR0822075.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHURQ_000192"
     CDS             complement(5571..6419)
                     /gene="folP"
                     /locus_tag="ACHURQ_000192"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR0822076.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHURQ_000193"
     CDS             complement(6509..8443)
                     /gene="ftsH"
                     /locus_tag="ACHURQ_000193"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR0822077.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHURQ_000194"
     CDS             complement(8543..9172)
                     /gene="rlmE"
                     /locus_tag="ACHURQ_000194"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR0822078.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHURQ_000195"
     CDS             9298..9591
                     /gene="yhbY"
                     /locus_tag="ACHURQ_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR0822079.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHURQ_000196"
     CDS             complement(9747..10223)
                     /gene="greA"
                     /locus_tag="ACHURQ_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR0822080.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10471..11904
                     /gene="dacB"
                     /locus_tag="ACHURQ_000197"
     CDS             10471..11904
                     /gene="dacB"
                     /locus_tag="ACHURQ_000197"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0822081.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11959..12066
                     /locus_tag="ACHURQ_000198"
                     /pseudo
     CDS             11959..12066
                     /locus_tag="ACHURQ_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHURQ_000199"
     CDS             complement(12090..13262)
                     /gene="cgtA"
                     /locus_tag="ACHURQ_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR0822082.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13278..14243)
                     /locus_tag="ACHURQ_000200"
     CDS             complement(13278..14243)
                     /locus_tag="ACHURQ_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0822083.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHURQ_000201"
     CDS             complement(14370..14627)
                     /gene="rpmA"
                     /locus_tag="ACHURQ_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR0822084.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHURQ_000202"
     CDS             complement(14648..14959)
                     /gene="rplU"
                     /locus_tag="ACHURQ_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR0822085.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15218..16189
                     /gene="ispB"
                     /locus_tag="ACHURQ_000203"
     CDS             15218..16189
                     /gene="ispB"
                     /locus_tag="ACHURQ_000203"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR0822086.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHURQ_000204"
     CDS             16417..16695
                     /gene="sfsB"
                     /locus_tag="ACHURQ_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR0822087.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHURQ_000205"
     CDS             complement(16743..18002)
                     /gene="murA"
                     /locus_tag="ACHURQ_000205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR0822088.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHURQ_000206"
     CDS             complement(18057..18311)
                     /gene="ibaG"
                     /locus_tag="ACHURQ_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR0822089.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHURQ_000207"
     CDS             complement(18471..18764)
                     /gene="mlaB"
                     /locus_tag="ACHURQ_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR0822090.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHURQ_000208"
     CDS             complement(18764..19399)
                     /gene="mlaC"
                     /locus_tag="ACHURQ_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR0822091.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHURQ_000209"
     CDS             complement(19418..19969)
                     /gene="mlaD"
                     /locus_tag="ACHURQ_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR0822092.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHURQ_000210"
     CDS             complement(19974..20756)
                     /gene="mlaE"
                     /locus_tag="ACHURQ_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR0822093.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHURQ_000211"
     CDS             complement(20764..21573)
                     /gene="mlaF"
                     /locus_tag="ACHURQ_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR0822094.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21783..22760
                     /locus_tag="ACHURQ_000212"
     CDS             21783..22760
                     /locus_tag="ACHURQ_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR0822095.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHURQ_000213"
     CDS             22774..23760
                     /gene="kdsD"
                     /locus_tag="ACHURQ_000213"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR0822096.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHURQ_000214"
     CDS             23781..24347
                     /gene="kdsC"
                     /locus_tag="ACHURQ_000214"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR0822097.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24344..24919
                     /gene="lptC"
                     /locus_tag="ACHURQ_000215"
     CDS             24344..24919
                     /gene="lptC"
                     /locus_tag="ACHURQ_000215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR0822098.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24888..25445
                     /gene="lptA"
                     /locus_tag="ACHURQ_000216"
     CDS             24888..25445
                     /gene="lptA"
                     /locus_tag="ACHURQ_000216"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR0822099.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25452..26177
                     /gene="lptB"
                     /locus_tag="ACHURQ_000217"
     CDS             25452..26177
                     /gene="lptB"
                     /locus_tag="ACHURQ_000217"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR0822100.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHURQ_000218"
     CDS             26225..27658
                     /gene="rpoN"
                     /locus_tag="ACHURQ_000218"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR0822101.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27681..27968
                     /gene="hpf"
                     /locus_tag="ACHURQ_000219"
     CDS             27681..27968
                     /gene="hpf"
                     /locus_tag="ACHURQ_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR0822102.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHURQ_000220"
     CDS             28086..28577
                     /gene="ptsN"
                     /locus_tag="ACHURQ_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR0822103.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHURQ_000221"
     CDS             28623..29477
                     /gene="rapZ"
                     /locus_tag="ACHURQ_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR0822104.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29474..29746
                     /gene="npr"
                     /locus_tag="ACHURQ_000222"
     CDS             29474..29746
                     /gene="npr"
                     /locus_tag="ACHURQ_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR0822105.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHURQ_000223"
     CDS             29960..30592
                     /gene="yrbL"
                     /locus_tag="ACHURQ_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR0822106.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHURQ_000224"
     CDS             complement(30589..31317)
                     /gene="mtgA"
                     /locus_tag="ACHURQ_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR0822107.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHURQ_000225"
     CDS             complement(31314..31967)
                     /gene="elbB"
                     /locus_tag="ACHURQ_000225"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR0822108.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHURQ_000226"
     CDS             complement(32197..34533)
                     /gene="arcB"
                     /locus_tag="ACHURQ_000226"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR0822109.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34629..35558)
                     /locus_tag="ACHURQ_000227"
     CDS             complement(34629..35558)
                     /locus_tag="ACHURQ_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR0822110.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36233..40693
                     /gene="gltB"
                     /locus_tag="ACHURQ_000228"
     CDS             36233..40693
                     /gene="gltB"
                     /locus_tag="ACHURQ_000228"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR0822111.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFCQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40706..42124
                     /gene="gltD"
                     /locus_tag="ACHURQ_000229"
     CDS             40706..42124
                     /gene="gltD"
                     /locus_tag="ACHURQ_000229"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR0822112.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42308..42712
                     /locus_tag="ACHURQ_000230"
                     /pseudo
     CDS             42308..42712
                     /locus_tag="ACHURQ_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42804..43109
                     /locus_tag="ACHURQ_000231"
     CDS             42804..43109
                     /locus_tag="ACHURQ_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0822113.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHURQ_000232"
     CDS             complement(43185..43649)
                     /gene="nanQ"
                     /locus_tag="ACHURQ_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR0822114.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHURQ_000233"
     CDS             complement(43646..44521)
                     /gene="nanK"
                     /locus_tag="ACHURQ_000233"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR0822115.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44518..45207)
                     /locus_tag="ACHURQ_000234"
     CDS             complement(44518..45207)
                     /locus_tag="ACHURQ_000234"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR0822116.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHURQ_000235"
     CDS             complement(45255..46745)
                     /gene="nanT"
                     /locus_tag="ACHURQ_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR0822117.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHURQ_000236"
     CDS             complement(46855..47748)
                     /gene="nanA"
                     /locus_tag="ACHURQ_000236"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR0822118.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHURQ_000237"
     CDS             complement(47870..48661)
                     /gene="nanR"
                     /locus_tag="ACHURQ_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR0822119.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49041..50363
                     /gene="dcuC"
                     /locus_tag="ACHURQ_000238"
     CDS             49041..50363
                     /gene="dcuC"
                     /locus_tag="ACHURQ_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0822120.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50406..50903)
                     /gene="sspB"
                     /locus_tag="ACHURQ_000239"
     CDS             complement(50406..50903)
                     /gene="sspB"
                     /locus_tag="ACHURQ_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR0822121.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50909..51547)
                     /gene="sspA"
                     /locus_tag="ACHURQ_000240"
     CDS             complement(50909..51547)
                     /gene="sspA"
                     /locus_tag="ACHURQ_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR0822122.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51942..52334)
                     /gene="rpsI"
                     /locus_tag="ACHURQ_000241"
     CDS             complement(51942..52334)
                     /gene="rpsI"
                     /locus_tag="ACHURQ_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR0822123.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52350..52778)
                     /gene="rplM"
                     /locus_tag="ACHURQ_000242"
     CDS             complement(52350..52778)
                     /gene="rplM"
                     /locus_tag="ACHURQ_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR0822124.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52997..54124)
                     /gene="zapE"
                     /locus_tag="ACHURQ_000243"
     CDS             complement(52997..54124)
                     /gene="zapE"
                     /locus_tag="ACHURQ_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR0822125.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54318..54716
                     /gene="zapG"
                     /locus_tag="ACHURQ_000244"
     CDS             54318..54716
                     /gene="zapG"
                     /locus_tag="ACHURQ_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR0822126.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54870..56237
                     /gene="degQ"
                     /locus_tag="ACHURQ_000245"
     CDS             54870..56237
                     /gene="degQ"
                     /locus_tag="ACHURQ_000245"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR0822127.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56327..57394
                     /gene="degS"
                     /locus_tag="ACHURQ_000246"
     CDS             56327..57394
                     /gene="degS"
                     /locus_tag="ACHURQ_000246"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR0822128.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14257 a        15304 c        14260 g        13607 t
ORIGIN      
        1 gcgcattatt ttacaaaaaa cacactaaac tcttcctgtc tccgataaaa gatgattaaa
       61 tgaaaactca tttattttgc ataaaaattc agtgagagcg gaaatccagg ctcatcatca
      121 gttaattaag cagggtgtta ttttatgacg acgattctca agcatctccc ggtaggtcaa
      181 cgtattggta tcgctttttc cggcggtctg gacaccagtg ccgcactgct gtggatgcga
      241 caaaagggag cggttcctta tgcatatact gcaaacctgg gccagccaga cgaagaggat
      301 tatgatgcga tccctcgtcg tgccatggaa tacggcgcgg agaacgcacg tctgatcgac
      361 tgccgcaaac aactggtggc cgaaggtatt gccgctattc agtgtggcgc atttcataac
      421 accaccggcg gcctgaccta tttcaacacg acgccgctgg gccgcgccgt gactggtacc
      481 atgctggttg ctgcgatgaa agaagatggc gtgaatatct ggggtgacgg tagcacctat
      541 aaaggaaacg atatcgaacg tttctatcgt tatggtctgc tgaccaatgc tgaactgcag
      601 atttacaaac cgtggcttga tactgacttt attgatgaac tgggcggccg tcatgagatg
      661 tctgaattta tgattgcctg cggtttcgac tacaaaatgt ctgtcgaaaa agcctactcc
      721 acagactcca acatgcttgg tgcaacgcac gaagcgaagg atctggaata cctcaactcc
      781 agcgtcaaaa tcgtcaaccc aattatgggc gtgaaattct gggatgagag cgtgaagatc
      841 ccggcagaag aagtcacagt acgctttgag caaggtcatc cggtagcgct gaacggtaaa
      901 acctttagcg acgacgtaga aatgatgctg gaagctaacc gcatcggcgg tcgtcacggc
      961 ctgggcatga gcgaccagat tgaaaaccgt atcatcgaag cgaaaagccg tggtatttac
     1021 gaagctccgg ggatggcact gctacatatc gcttatgaac gtctgttgac cggtattcac
     1081 aacgaagaca ccattgagca gtatcatgca catggtcgtc agttgggccg tctgctgtac
     1141 caggggcgtt ggtttgattc ccaggcgctg atgctgcgtg actctctgca acgctgggtt
     1201 gccagccaga tcactggtga agttaccctg gagctgcgcc gtgggaacga ttattcaatc
     1261 ctgaataccg tctcagagaa cctgacctac aagccagagc gtctgacgat ggaaaaaggc
     1321 gactcggtgt tctcgccaga tgatcgtatt ggtcaattga ccatgcgtaa cctggatatc
     1381 actgataccc gagagaaact ttttggttat gccaaaactg gcctgctctc ctcctctgcc
     1441 acttcaggcg tgccgcaggt ggagaatctg gaaaacaaag gccagtaatt cgacatcaac
     1501 cctgcccttc aggggtgggg ttaccttgct tatcctctgt aaactccttt ctgagcatca
     1561 cataatcgaa aacatcatgg tttgcatcca caacccgtga gtctccttta tagtgtgcaa
     1621 tcacttcctc cagcgttttc ggttgttccg gctttgttac ccccatccac gcattaataa
     1681 ggtaattatt gtaagctgtc gaaaagatgt tgttttccgt tgtacgatcc acgccatccc
     1741 caagaacggg gctataccag ataaacatcg ggacatgata tgcctgctgg ctagcctccc
     1801 tgcctccatg aaaatagacg ttcttcttcg tagggtcacg ttccaggccg tggtcggcaa
     1861 aatacatgac cgaggcgcgg cgatctttta ataattcaaa aacctgacct agcaaactat
     1921 ctgtgtaatg aatggagttg tcatagcagg catcctgatc gtcctgcggt tgaaacacgg
     1981 cgctggattg cggataggcg ctacaagccg gttcatggct tccatttaaa tgaagaacaa
     2041 tcagtttctt ttgctgcgta ttttgctgta atgcttgcga taaatgcggc aacaacaatt
     2101 catcaaatcc tctgacataa actgtttcca tggcgcgcat ggcgatactg gtaactgctg
     2161 taccattctg ccgaaaagcg gattgcgagc ttagccagaa agtctgaaat cctgcttgat
     2221 tagccatatt aataatgttg tcggggtaat tatgaatgtc atgactcaaa acagaatcag
     2281 cagttaaaga aaggggaacc gacagcgcag tgtaaggtgc gccgcttatt gattgattaa
     2341 acagtttgat ctgttttctt tgtgcttcaa cttgcggtgt cgtagagcgt gtatatccat
     2401 acaaagacat attgtcgaca cgtacagact cccccacaat caacacgtag gtatcaatac
     2461 ctgtatccct gactgataat tgaaaatacg gcaccgtgtt tgcaattgag agtaatcttt
     2521 gatgctcttt cgctgctaaa gcaaaataat tgagattgaa aaacggcgta taggtagcaa
     2581 atcgcgacgc tagtatatat ggactgaatg cttttttatt ttttgcatct ttataagcaa
     2641 attgacaagc agaaaataaa ctgcccgaaa tgacaatcag caataatatt cctgtcactt
     2701 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
     2761 aagcaaaagc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
     2821 cactctgcag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
     2881 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
     2941 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
     3001 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
     3061 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
     3121 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
     3181 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
     3241 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
     3301 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
     3361 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
     3421 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
     3481 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
     3541 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
     3601 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
     3661 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
     3721 ggctgagttt gttcggtttt cgccggtgca ctcagatttt cccattcgct acctttattg
     3781 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
     3841 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
     3901 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
     3961 ccaacaaggc caattgccac aataaggaaa actaccaaaa gagcttcgta cataatcaac
     4021 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
     4081 ctttcccact gaagtgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
     4141 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
     4201 aggcgacaat cgccgccttt ttagccagtt atttaccgtt ttaaacggct tttactgcat
     4261 cggcgatacg gtgtgcaaat tcagttacct gcgcttcgtc ttcaccttct accatcacgc
     4321 gaattaacgg ttcggtgccg gatttacgca acagcacacg tccacggcta cccagcgccg
     4381 cttcaacttc ggcggtcaca gctttaactg actcatgctc aagcggatcg ccgctacctg
     4441 cggtgtaacg cacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
     4501 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
     4561 caccggtagt ggttttatcc agcaggatca catgaccgga attctctgca ccgatacgcc
     4621 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
     4681 gaattcctaa ctgtttcaac gccagctcaa gccccatgtt actcatcaat gtacccacag
     4741 caccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
     4801 caccatcgac tttattgcct tcatggtcaa ccataatcac gcgatcgccg tcgccgtcga
     4861 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
     4921 tagcccccac ttcggcattg atgtttacac cgttcggctc acaaccgata gcgataacgt
     4981 tcgcccccag ttcacgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcgcaat
     5041 ccaccacaat cttcagttcg ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
     5101 cgatatagcg acccgcagca tcaacgatac ggctggcttt acccagttct gccgaatcaa
     5161 cacagctgat ctctttttcc atttccgctt cgatggcctc ttctaccgca tccggtagtt
     5221 tggtgccgtc gatagagaaa aatttaatgc cattatcgta gaacgggtta tgcgatgcag
     5281 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
     5341 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
     5401 actccagcat atagccagaa atacgcgtgt ctttaccaat aatgatctta cgggagccgt
     5461 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
     5521 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
     5581 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
     5641 ttgacgtcat gaacacgaat aatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
     5701 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
     5761 cgtgacatac ctaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
     5821 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
     5881 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
     5941 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
     6001 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
     6061 agggagcgga tatcgttaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
     6121 ggtttggatg tatctaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
     6181 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
     6241 tcgccaccaa cgtcaatgat cgtcgcgcca gcgttgatca tcagattcgc atgtttcacc
     6301 gcatctatca gcgagttatg cgtgccacca tccgaaaagg aatcaggcgt gacgttgagg
     6361 atccccatta cgtgaggatg gctaaggtcc agtgaagtgc cctgggcaaa gagtttcatg
     6421 gtgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
     6481 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
     6541 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
     6601 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
     6661 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
     6721 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
     6781 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
     6841 tcgctttcgc tacgctacgg ccgaggaaca cttcaccttc ttcttccgcg tacagcagtg
     6901 gacccaattt ctcagagaag ccccactgag tcaccatgtt acgcgccagg ttggtcgcaa
     6961 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccgtagatg atctcttctg
     7021 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
     7081 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
     7141 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
     7201 cgtggtaagc cgtcgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
     7261 ccatcatgat tttatctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
     7321 cacgagcagc gaacagtgcc gcttcgttta ccaggttcgc caggtcagca ccggagaaac
     7381 caggagtacc acgggcaatg attgccgcgt cgatatcggg tgccaatggt acgcgacgca
     7441 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccaacc acaacctgac
     7501 ggtcgaaacg gccaggacgc agcagggccg ggtcaagaac gtccggacgg ttagtcgcgg
     7561 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
     7621 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
     7681 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
     7741 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
     7801 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcagcgtt ttaccggtac
     7861 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
     7921 tcggctcgcg cagatattca accagttcag caacttcttc ttttgcttcg tcgcagcccg
     7981 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
     8041 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaca
     8101 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
     8161 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
     8221 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
     8281 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
     8341 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
     8401 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
     8461 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
     8521 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
     8581 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
     8641 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
     8701 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
     8761 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
     8821 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
     8881 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
     8941 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
     9001 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
     9061 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
     9121 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
     9181 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
     9241 tatgggagat ggcggtagaa tgacccattt tcaatcccaa cgtaagcaaa aaatacgatg
     9301 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
     9361 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
     9421 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac aaaaaccttg
     9481 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
     9541 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
     9601 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
     9661 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
     9721 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
     9781 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
     9841 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
     9901 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
     9961 aaaataacgc gcccattgtt aggcattttg gtgacatcaa tcacctgcgc gtttgacagc
    10021 ttggcttcga tgtctttgat acggccttcg cagaagccct gctgttcacg agctgcgtgg
    10081 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    10141 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    10201 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    10261 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    10321 aaaataccga cccaggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    10381 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    10441 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    10501 accagctgta tagcgttcag tgttcaggcc gcaaatgttg atgagtacat tactcaactc
    10561 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    10621 gattaccaca gtcagcagat ggcgctgcct gccagtaccc agaaagtgat tactgcgctg
    10681 gcggcgttga ttcaactcgg ccccgatttt cgttttacca cgacgcttga aaccaaaggc
    10741 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    10801 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    10861 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    10921 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgccgc catagttgac
    10981 cgcaactgtt tctccgtctc gctctacagt gcccaaaaac ccggtgatat ggcctttata
    11041 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgtggt
    11101 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    11161 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    11221 ggagccagtt atgccggtgc aattctgaaa gatgagttaa aacaggcggg tatcacctgg
    11281 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    11341 cagtcggccc cgctgcacga tctgcttaag attatgctga aaaagtcgga taacatgatc
    11401 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtacctgg tacatggcgg
    11461 gccgggtcgg acgctgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    11521 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    11581 atgcaggtgc tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    11641 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    11701 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    11761 attaccacag cgagcgggca acgaatggcg tttgtgcaat atctttctgg ctatgcagta
    11821 gaacctgcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    11881 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggcttcag
    11941 attattgaca aagtgcgctt tgtttatgcc ggatgcggcg taaacgcctt atccggccta
    12001 caaaatcgtg caaattcaac atattgcaat tctcttgtag gcctgataag cgtagcgcat
    12061 caggctgatt tggcgtttat catcagtgat taacgcttgt aaatgaactc aacgccttct
    12121 tcgtcgtctt cgtcccagtc gtcatcccag tcttcatcat cctcttcagc aatctcttca
    12181 agctgctggc gatgataatc gtcccacatg aattcgactt tctctggctg tttcgcttct
    12241 tcagcctgca cgaccgggtt ttcaaggata aaggtcatca catcccagca gagatctttc
    12301 acgcccagtc cgctcgccgc agagatcaga taatatttat cttcccagcc cagcgcttca
    12361 gcaatcgctt tcgctttctc ttcagcttct gccttatcca gcagatcgat cttattgaac
    12421 actaaccaac gcggtttcgc cgccagatcc tggctgtatt tttccagctc gctgataata
    12481 atacgcgcgt tttcaaccgg atcggtgccg tcaatcggat cgatatcgat gaggtgcaac
    12541 aggacgcggc aacgttccag gtgcttcagg aagcggatac ccagacctgc gccttccgca
    12601 gcgccttcaa tcagtcctgg aatatcggca acaacgaagc tcttttcgtt gtccattcgt
    12661 accacaccca gacttggcac cagagtggta aacggataat ccgccacttt cggtttagcc
    12721 gccgataccg cgcgaataaa ggtcgattta cccgcgtttg gcatccccaa cataccgacg
    12781 tcagccagca gcatcagctc cagcagcagc tcacgcttat cacccggcgt accgttggtt
    12841 ttctgccgtg gtgtacggtt aacggacgat ttgaaacggg tattgcccag accgtgccag
    12901 ccgcccttag caaccagcag acgctgaccg tgtttagtca tatcgcccat ggtttcacca
    12961 gtaccctggt cgattacacg cgtacctacc ggcactttaa tcgtcacgtc tttaccgcgc
    13021 ttaccggtac aatcgcggct tgcgccgttc tgaccgcgct ctgcacggaa agatttttca
    13081 aaacgataat cgataagcgt gttcaggttt tcgtcggctt ccatccatac gtcaccacca
    13141 tcaccaccgt cgccgccatc cgggccgcct ttcggaatat acttttcgcg gcggaagctc
    13201 acgcaaccat taccgccatc acctgcaacg accagaatcg atgcttcatc aacaaacttc
    13261 attttattct ccataaatca ttcgcctgcg cggggttgcg ataccaccgt tgtatgctta
    13321 cgtaatccgc cccaaatacg atgaccaatg gcggaataca tcgcgcccgc aaccacgaca
    13381 aacgcaccga gataacctaa aaggtttaac atcggtctgg cgaagaaatc gggccaggcc
    13441 agtgataaaa gatctgaaaa aaacagcgta aacagtgggg tgagcgtgat gatcgcgctc
    13501 acctgcgctg cctgccagcg agccatcgct tccgccaggg cgccatatcc taccaatgta
    13561 ttcagtccgc aaaaaattaa acatgcgagc tgccagtggc taagctgcgc tatcactcca
    13621 ggctttgcca gagggaagag cgcaattgta cataaagtgt acagtaaaaa caggatctgc
    13681 ggtgaggcca gccgacgcaa taaaaccttt tgcgccacgc cataactcac ccaaaccgtc
    13741 gccgcaccga ccccaaagat aactccccag gtgtaatcgg tgagctttgt aaatatctcg
    13801 accagactgg tgttaaaaaa catcaccagg ccgctcagga gcatcaatgc ccctacaacc
    13861 tgagtgctgc gcattttctc tttcaggata aatacgctgg caaccatcat gccaactggc
    13921 gagagttgcc caatcacctg cgaagcggtc ggactcaggt attgcaagga tgagctgaac
    13981 aggatgaagt tcccaaacag cccggcggtc gccactgcca acaaaatcaa ccagcgtggc
    14041 ttacgaaaca cgcgtaatgg cggcaacctc ttcttcaccg caagaatggc accaaggcca
    14101 atactcgcca tcaagaaacg gtaaaacacg attgtcggag gttccatcac ctccagcacc
    14161 tgcttcattg cgattggcaa cgccccccag caaattgctg tggtgagcgc caaaagaatg
    14221 ccaatgcctg cctgctgctt cataccgtat tccctgcaaa gcgcatttgc ggattaacgt
    14281 cgcaccatgc gcatttttac cggttatcga atgtaaaaag ccccgcaacg tgttgcgggg
    14341 ctttcatccg ttaccgggac gcgaaaaact tattcagctt cgatgctgat aaatttacgg
    14401 tttttcgggc ctttaacttc gaatttcact ttaccgtctg ctttagcaaa cagagtgtgg
    14461 tcacgaccgc aacctacgtt agcgccagcg tggaatttgg taccacgttg acgaacgatg
    14521 atgctacccg ccagaacgga ttcgccaccg aaacgcttaa cgcccaggcg tttagcttct
    14581 gaatcgcgac cgttacgtgt ggagccgcca gcctttttat gtgccatttg aaatctctcc
    14641 tcaggtctta ggcgctgatg ccagtaattt tcacatcagt gaaccactga cgatggccct
    14701 gctgcttacg atagtgttta cgacgacgaa acttaacgat tttaactttc tcgccacgac
    14761 cgtgagcaac aacttcagct ttgattacgc cgccatcaac gaaaggaacg ccgattttga
    14821 cttcttcacc gtttgcgatc atcagcactt cagcgaactc aacagtttcg ccagttgcga
    14881 tgtccagctt ttccaggcga acggtctgac cttcgcttac tcggtgttgt ttaccaccac
    14941 tttggaaaac cgcgtacata taaaactccg cttccgcgca caccttttca atgattcaga
    15001 gtgcgctata aatattcaca atagggcgcg aatattacgc aaaacgcacg cctttgacaa
    15061 gtgctacagt caatacacga agaaaaaaaa cacaacttgt acggtaacgt ttatctgtgc
    15121 cattttttca gtacaatcac cctatattcc taaccataaa ccctaagttg cctttgttca
    15181 cagtaaggta atcggggcga aaagcccggc ttttgcgatg aatttagaaa aaatcaatga
    15241 gttaaccgcg caagatatgg cgggtgttaa tgcggcaatc cttgagcagc ttaattccga
    15301 cgtccaactg atcaatcagt taggctatta catcgtcagc ggcggcggta aacgtattcg
    15361 tccgatgatt gctgtactgg ctgcacgagc tgttggctat gagggaaatg cgcatgtcac
    15421 cattgctgcc ctgatcgagt ttatccacac ggcgactctg ctacacgacg acgttgtgga
    15481 tgaatcagat atgcgcaggg gtaaagctac cgccaacgcc gcatttggca atgccgccag
    15541 cgtgctggta ggcgatttta tttatacccg cgcgttccag atgatgacca gcctcggttc
    15601 actcaaagtg ctggaagtca tgtcagaagc tgtaaacgtc atcgcagaag gtgaagttct
    15661 gcaactgatg aacgttaacg atccggacat cactgaagaa aactacatgc gcgttatcta
    15721 tagcaaaacc gcgcgtctgt ttgaggctgc cgcgcagtgt tccgggattc tggctggctg
    15781 tacgccggag gaggagaaag gcctgcagga ttatgggcgc tatctcggca ctgctttcca
    15841 gttgatcgac gatttactcg attacaatgc cgatggcgaa cagttaggta aaaatgtcgg
    15901 cgacgatctg aacgaaggta aaccgacgct gccgctgctg catgcgatgc atcatggcac
    15961 accagaacag gcacagatga tccgtaccgc catcgaacag ggtaacggtc gccatcttct
    16021 ggaaccggtt ctggaagcaa tgaacgcttg tggatctctt gaatggacgc gtcagcgtgc
    16081 cgaggaagaa gcagacaaag ccatcgcagc attacaggtg ctcccggaca ccccttggcg
    16141 agaagcactc atcggcctcg cgcacatcgc tgttcaacgc gatcgttaat cccctccctt
    16201 catcccgcgc agtgcgcggg atgattccag taaattccat aaaaacacta attcagcgct
    16261 ataacacctg aaaaaaaggg cgatgaatct ttatatattc tgaatattca cactctttac
    16321 aggaactttt tagagcaata ggccatcagg agtatagtga tgcccgacag aagaagtgtt
    16381 ctgaatgaaa gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg
    16441 catcccgctg acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggagtct
    16501 cgcagaaatg gtttgagttc ctcaacgctg gcaaacgcat tatcgcgccc atggccgaaa
    16561 ggagagatga ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc
    16621 taccatgatc cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact
    16681 aaaccgaaaa aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac
    16741 gattactcgc ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg
    16801 cgttcgtagc cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata
    16861 cagccagcca gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca
    16921 gaaagttttt caacaccgtg acaaataacg gtattgcttt cgatttcggc atgcgcgccc
    16981 atacggctaa gctccggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat
    17041 ccggtccctt ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat
    17101 gccggatgcg gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg
    17161 ctaatccagt cttcgccgac ttcgatgtcc gctccagcgt cacgcagttt cgccagcacg
    17221 gcgtcgagag tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc
    17281 gccaccagga aagtaccggt ttcgatacga tccggtagca cgcgatagac accgccgcct
    17341 aaacgttcca caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc
    17401 agcgtaatca ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata
    17461 atcgtggtac cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg
    17521 actttatcca tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa
    17581 ccttcttcca gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc
    17641 ggacgcgcac cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct
    17701 accagcggcc ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg
    17761 cagaatacat taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca
    17821 cccagctggc ttagcagctt cattgatgta tcgacgtctt tcagttttgg gacgttctgg
    17881 atctctaccg gttcttccgc cagtagagcg gcaaaaagga taggcagagc agcattttta
    17941 gcgccggaaa ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc
    18001 atttagtttg ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa
    18061 aagccgttca gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca
    18121 gcatgaatgc ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt
    18181 ttaacccgac tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca
    18241 tcgccggaaa cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca
    18301 ttattttcca tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa
    18361 gttgcgctgg cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc
    18421 aggcaggttt gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc
    18481 agaacatcag caggcaaatt atacaatttc gcgagggtat acactttgtc gtttaccccc
    18541 tgaagcgtca cgttgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc
    18601 agtccacccg tatccacgcg ggacacacgg ctaagatcga tgcaggtaat ccccttcacc
    18661 gcttcctcac gcatttccca aagcggtagc agaacgtcct gatccagctc tccggataac
    18721 gccagcgtgt cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca
    18781 gagtgatttt ctgttgagaa atcgatttca gttgcgcagt caggccgtcg atacctttgg
    18841 tacgcagcag cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa
    18901 tcatgtcgta agcctgccag tttcccgtct gggagttttt acgccactgg aagtccagac
    18961 gcaccggcgg acggccattc gggtcaataa tggtaacgcg aataggcaca atggttttat
    19021 cgcccagcgg ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct
    19081 gaccgtaagc ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag
    19141 gggtcgcact cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt
    19201 atggcagcag ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg
    19261 gttgctcatt cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttataag
    19321 gattggtctg gtctgcctcg gttgccgcac tcagaggtgc aatcaccagc aaagcgacca
    19381 tcattaaacg tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt
    19441 cagtggtttc attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat
    19501 cgcctttact accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag
    19561 tgtcctgaat tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac
    19621 cgacgtttaa tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac
    19681 tggtatctgg aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg
    19741 ttttcgggtc cagcgtaata tccgccaccc gacccacaac aacgccacca atactgaccg
    19801 gagagcgcgc tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt
    19861 cagtacgtat ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg
    19921 ctgctaataa aaagataccc acccaaattt catttttttt cgtttgcatg aactcaattc
    19981 ccaaacatca atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca
    20041 acggtgcgag tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta
    20101 aacaacgaaa tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc
    20161 agatccatac gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg
    20221 cctttccaac tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc
    20281 gtcaacagtg gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc
    20341 ggatccaccg ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt
    20401 tctgcggtta gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc
    20461 agttcacgca gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg
    20521 gtcagaacca gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca
    20581 ataatcagca tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt
    20641 ttgcgaaatt ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac
    20701 gttctcaggg ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt
    20761 ggcttaactc cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac
    20821 aggcccgtca gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa
    20881 cgcctgggca ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc
    20941 aatacttaac acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct
    21001 gttcagctca gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa
    21061 aggttcatca aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg
    21121 ccgcgccatc ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac
    21181 ggcctccagc ttcatcatca ccgtactatg caacaatggc gcgggtagtt gggtatgttc
    21241 gcgcagtgga taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccggactg
    21301 aaataacatg ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat
    21361 attctcacca tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg
    21421 gagtagcgtc gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg
    21481 cacggtcagg gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg
    21541 catatcgact aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt
    21601 tctaagcatg gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg
    21661 tggttagcga aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat
    21721 ccgttacggc tttctgaaaa tcttcagcgg accgacgagt atacctgaag aaaggacgtt
    21781 agatgctttt agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg
    21841 accgcctggt ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca
    21901 tcggcatgac ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg
    21961 cgtctctgca cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca
    22021 atattttgct gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattccgatg
    22081 ttctgcgtcg tgaattaccc ttaatgttgt tggtcagcgt agtggccggt tccgtactct
    22141 atgacggaca acttagtcgc agcgatggta tctttctcct ctttctggct gtgctatggc
    22201 tgctgttcat tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca
    22261 gagagcagct tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg
    22321 gcattgcgct tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc
    22381 tggcgaatta ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa
    22441 ccagcctgcc ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg
    22501 ctgtcggaaa tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg
    22561 cgctgataac gccaggagag attgagccat tggcgtacag tcgtgactac agcgtgatgt
    22621 tgctggtgag cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg
    22681 gtgtaggggt attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat
    22741 cgccaatact cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt
    22801 tttgactttc agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag
    22861 cttgatcaat acatcaatca gaatttcacg cttgcctgtg aaaagatgtt ctggtgtaaa
    22921 gggaaagttg tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa aatggccgca
    22981 acgtttgcca gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt
    23041 gatttaggca tggtcacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc
    23101 agcgaaatca cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc
    23161 accggtcgcc cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta
    23221 gcgaaagaag cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt
    23281 atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt
    23341 gcgctctcac acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt
    23401 atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcgttg
    23461 ctggaagtta cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt
    23521 gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt
    23581 cagttaagta ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc tggcattctg
    23641 gccgttgagg cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat
    23701 ggcgaccatt tactcggagt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa
    23761 agattcaagg ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc
    23821 ctgtcagcgc cgacgttata gcaaaagcag agaacattcg tctgctgatc ctcgatgtcg
    23881 atggcgtact gtcagatggc ctgatttata tgggcaataa tggcgaagag ctgaaagcgt
    23941 tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta
    24001 tcattaccgg gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc
    24061 acttgtatca ggggcagtca aacaaactga tcgcctttag cgatctgctg gaaaaactgg
    24121 cgattgcccc ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg
    24181 aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg
    24241 attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac
    24301 tcctggcgca gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg
    24361 ttgggttatc attgtgctat cactggcggt tctggtgatg atcggcattg atatggccga
    24421 aaaagacgat accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca
    24481 tacggacacg ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca
    24541 cgttgaatat tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt
    24601 tgataaggat aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga
    24661 ccggatgctc tatttatatg gacacgttga agtcaacgca cttgtgccag actctcaact
    24721 tcgcagaatc acgacggata acgcgcagat caatctggtg acgcaggatg ttacctctga
    24781 agacctcgtc acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa
    24841 cttacgcagc aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa
    24901 caaacaaact cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg
    24961 catttgccgt aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc
    25021 ttgatatgca aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca
    25081 tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag
    25141 tgattgacgg ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg
    25201 ttgaaggtca cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg
    25261 gtaatgctta tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg
    25321 taaaagagca gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc accgttctgg
    25381 tgccgtcgca gctgcaggac aaaaacaaca aaggccagac cccggcacag aagaagggta
    25441 attaattcgt tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc
    25501 gcgtggtaga agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc
    25561 caaacggtgc cggtaagacc accactttct acatggttgt aggcattgtg ccgcgcgatg
    25621 cgggcaacat cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc
    25681 gccgcggtat cggctatctg ccacaggaag cctccatttt ccgtcgcctc agcgtttacg
    25741 ataacctgat ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc
    25801 gcgcgaacga gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt
    25861 cactctccgg gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga
    25921 aatttattct gctcgacgaa ccgtttgccg gggttgaccc gatctcggtt atcgacatta
    25981 aacgcatcat tgagcacctg cgcgacagcg gcctgggcgt gctgatcact gaccacaacg
    26041 tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg
    26101 cccacggcac gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg
    26161 aagacttcag actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct
    26221 gaacatgaag caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct
    26281 ccaacaggca attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca
    26341 ggcgctggag agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg
    26401 cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc
    26461 ggaagagctg ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg
    26521 caccagcggt gactacattg acgatgagct gccggtctat cagggcgaaa cgacgcagac
    26581 cttgcaggat tacctgatgt ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc
    26641 tattgctacc tctatcgtcg atgccgttga tgacatcggt tatctgactg tcccgctgga
    26701 agatattctc gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg aagccgtcct
    26761 taagcggatc caacggtttg atccggtcgg tgtagcggca aaagatctgc gtgactgcct
    26821 gctaatccaa ctctcccaat tcgataaaac cacgccgtgg ctggaagagg ccagactgat
    26881 cattagcgat catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac
    26941 gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg
    27001 ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg
    27061 taagcataac ggtcactgga cggtagaact caacagtgac agcattccgc gtctgcaaat
    27121 caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac gatggtgaca gccagtttat
    27181 ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac
    27241 gctactgcgc gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggtga
    27301 agaatatatg aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc
    27361 gacgatatct cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact
    27421 gaagtatttc ttttccagtc acatcaatac cgagggcggc ggcgaagctt cctccacggc
    27481 gattcgtgcg ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga
    27541 cagcaagtta acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc
    27601 gaagtaccga gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc
    27661 caaccgataa ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg
    27721 aggcactgcg cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa
    27781 tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac
    27841 tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca
    27901 ttgatggctt aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac
    27961 aacactaatt gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc
    28021 catttgtacg gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg
    28081 aaattatgac aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta
    28141 cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg
    28201 cggcgaaaca acttagcctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa
    28261 aaatgggcag taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag
    28321 atactctgcg cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca
    28381 tcgacaacca accggtggac ttactttttg ccctgctggt gtcggcagac caaactaaaa
    28441 cgcacctgca tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc
    28501 gtttacgcgc agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta
    28561 ctccggatga agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt
    28621 acatggtact gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg
    28681 cgctggaaga tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc
    28741 tggctcgaac cctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata
    28801 tgccggagtc accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac
    28861 cgcaactcct gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc
    28921 gccgactgca tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa
    28981 gcgatttgct ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt
    29041 ccgttcacga gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac
    29101 tgaccatggt ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg
    29161 tctttgacgt gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag
    29221 gtcttgataa acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct
    29281 accagacgcg aagctatctt gagctatggt tacctatgct ggaaaccaac aaccgtagct
    29341 acttgacggt cgccattggt tgtaccggcg ggaagcaccg ttcggtgtat attgcagagc
    29401 aactggcaga ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg
    29461 aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca
    29521 tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt
    29581 acgtaatgac gaaggcaccg aggccgaagc caacagcgtt attgcgctgc tgatgttgga
    29641 ttctgccaaa ggacggcaga ttgaagttga agcgaccggc ccacaggaag aggaagcact
    29701 ggccgccgtt atcgccctct ttaactctgg ttttgatgaa gattaatctt catcactttg
    29761 acatacaact cccttcaaac tccccccgac aataagaaaa tcacgtactg aaatcgttct
    29821 caatcaacgt catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac
    29881 cttaagaggc attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa
    29941 aatatttcca ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag
    30001 acatcggaaa tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca
    30061 tcgtggcgat ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg
    30121 tcgccggtta aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg
    30181 cggaaccggt tatgtctacg atgtgattgc agattttgac ggcaaactct ccattacgct
    30241 gaccgaattt gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact
    30301 gaaacagctg aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca
    30361 gaatatcctc tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gcgacaacat
    30421 tggtgaaagc acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca
    30481 agagcggtta tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga
    30541 tctgcaacct cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg
    30601 gtgttgttgc ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg
    30661 acggctacgc acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac
    30721 agctgccagt aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg
    30781 ttgtgccgca gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac
    30841 cgtcaggata cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag
    30901 cccttttcgt acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga
    30961 aatcgttgaa gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc
    31021 aatggaagcg acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac
    31081 cgccagtccc atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta
    31141 acgaaaattg ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg
    31201 aacaggcgca acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat
    31261 taacagcaaa agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca
    31321 gccagaacca gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc
    31381 tgcgccagca tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca
    31441 tcgacaggac agggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata
    31501 tcagtaccga tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca
    31561 atacacataa aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat
    31621 tcacggtcaa cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt cttcgccgcg
    31681 ccaaaccccc ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga
    31741 cggatttcac cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt ttccgtcatc
    31801 gcttcgccag ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc
    31861 gcctgcgcac cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa
    31921 ccgtcataga cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt
    31981 tcgcaattga ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa
    32041 ctgattcatg taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac
    32101 agtgctgtgc ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg
    32161 ttggcgcagt attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac
    32221 ccacgctttc agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc
    32281 accgacgtta tcttcccagg caggaaggtc aggagactga atttgctgac ccagctgttg
    32341 cagatggcgt aaccccactg accccgccgc acctttaatt ttatgtcctt cctcaacaat
    32401 gcctttttta tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat
    32461 cttctcaaac accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg
    32521 ttccagcatg ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt
    32581 actctcctca tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac
    32641 agaaagcggc ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt
    32701 cagcacgtta gcggttaagg ccaccagcgg cggtaaatcc tctcgcggat aacgcttcgt
    32761 cagttcacga gagatatcca gcccggtcat atctggcaac tgaatatcca gtaacaccag
    32821 gtcgtattcg cccggtttaa acatctctag cgccgccttg ccggtcatgg cgacatcaac
    32881 gctgttacct aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc
    32941 caccagcagc acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac
    33001 ctcttctgct accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc
    33061 gctggtaacc gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc
    33121 aataccggtg ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc
    33181 gaaaatttta tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg
    33241 cagcatatcg ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt
    33301 gacggcgtta ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac
    33361 ctgatgcggt aatggcagcg tcggctccag gttaaagcgc aatccttttt gttgcgcctg
    33421 caatgcggag agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc
    33481 aagctggacc ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc
    33541 cagcgtgacg gccgaaacat ggatggtctt gagatatttt tcctgctcgg cggtgagttc
    33601 ggtatccagc agaatgcggc tcagaccgac gataccgttc agcggtgtac gcaattcgtg
    33661 actgatggtg gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata
    33721 ccgcttacgc tcggtaatgt ctcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg
    33781 gtcgtagtac ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag
    33841 ccactgttca taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac
    33901 ttttgcggcg gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc
    33961 gctttttccg gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt
    34021 acgataaaaa accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg
    34081 ctcgagctga atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt
    34141 ttcctgcagt tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat
    34201 attatctttt aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg
    34261 caccagccgt gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa
    34321 gtagaccgcc cacggcgtaa tcaacaaacc aaagaagata gaacgaataa catcaatgct
    34381 ttcgacctga ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac
    34441 gagggccagc gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac
    34501 ataatactgc gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc
    34561 ccaataatac ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc
    34621 tcaacgacct actccgttgg aggtagccac ggacgtccca gcgccgatcc ctgtacgcca
    34681 tgttcattca gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacaga
    34741 ggagcaagca gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt
    34801 ggcgtatggc gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc
    34861 aaacgccccg cttcccaggc tttcgccata atgctgcctt tcacaatatg caacggatgt
    34921 agctttatac cgtcaacacc ggtttccact acccgctcca gcgtttgcag acattcagcc
    34981 agcccttcgc ctggcagacc gacaattaaa tgcgagcaca ccttcagccc gcgctggcgt
    35041 gccagctgcg tcgtgcgctg ataacaggcg aaatcatgac cgcgattaat gcgatgtagc
    35101 gttttgtcgt gcacggtttg cagccccagc tccagccaca cttcgtagcc ctggtcttta
    35161 tattcgcaaa gcagatccag caccgcatcc ggcacgcagt ccgggcgcgt gccgacacac
    35221 aaaccgacaa tattggcctg gctcaccgcc tgttgataca tcgaacgcag cacctgaact
    35281 tcggcaaagg tgctggtata cgcctggaaa taggcgaggt aacgtttcgc gcgattaatt
    35341 aaattcgcct gatgcgccag ctgttcggca atggaacgat gctgctgcgc ttcgtcggca
    35401 aacgaggcaa cattacagaa ggtgcagccg ccgcgcccga tggtgccgtc gcgattcggg
    35461 caactgaaac cgccgtgcag cgtcagctta tgcacctttt gcccataacg acgggtgaga
    35521 tcaccaccaa acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga
    35581 aaagaggcca aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc
    35641 ggcccgcttt tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca
    35701 taaatcactt ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa
    35761 aacagtagca tgaaacgtca ttaccaatta atgcagtata aaatgctggt tttctcgtca
    35821 attcaaggca ggataagggt taacacgcct ttatgacagt caggaattga ctgtttctct
    35881 aacgacttcc cttttagcct taaagataaa atctatttta atttcagcca tttaataaag
    35941 aattttgcgc taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt
    36001 tcccgataag ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt
    36061 atgcagtaat tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg
    36121 gcgaatgcga ggcgcgcgta tgacacgcaa cccccgtcgc cacgctcttt ctgtacccgt
    36181 gcgcagcggt tcggtagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta
    36241 cgataaatcc cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga
    36301 acctagccac aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg
    36361 cgcgattctc gccgatggta aaaccggtga cggttgcggc ttgctgttac aaaaaccgga
    36421 tcgctttttt cgcatcgttg cgcaggagcg cggctggcgt ttagcgaaaa actacgccgt
    36481 cgggatgcta ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga
    36541 agaagaactg caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga
    36601 aggcgtactg ggtgaaatcg ccctctcctc tctgccccgc attgagcaaa tttttgtgaa
    36661 cgccccggca ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg
    36721 cattgaaaag cgtctcgaag ccgacaaaga cttctacgtc tgtagcctat cgaatctggt
    36781 gaacatctat aaaggtctgt gtatgccggc ggatctgccg cgcttttatc tggatcttgc
    36841 ggacctgcgt ctggaatcgg ccatttgcct gttccaccag cgcttctcca ctaacaccgt
    36901 accgcgctgg ccgctggcgc aaccgttccg ctatctggcg cataacggtg aaatcaacac
    36961 catcactggt aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgcttat
    37021 ccctgatctg cacgacgccg caccgttcgt caacgaaacc ggctctgact ccagttcgat
    37081 ggataacatg ctggaactcc tgctggcagg cgggatggat atcatccgcg ccatgcgtct
    37141 gttagtgcca cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt
    37201 tgactttaac tccatgcata tggagccgtg ggatggcccg gcgggcatcg tgatgtccga
    37261 cggtcgtttt gccgcctgta acctcgaccg taacggtctg cgtccggcgc gctacgtcat
    37321 caccaaagat aagctcatca cctgcgcctc tgaagtcggt atctgggatt accagcctga
    37381 cgaagtggtc gaaaaaggcc gcgtcgggcc aggcgaactg atggttatcg acacccgcag
    37441 tgggcatatt ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc atccatataa
    37501 agagtggatg gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga
    37561 agtgggtagc cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta
    37621 cagcgcggaa gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt
    37681 cggttcgatg ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta
    37741 cgactacttc tgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga
    37801 agcgcatgtt atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc
    37861 agagggccag gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa
    37921 acagctcacg acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga
    37981 cgtcactaaa accacgctcg aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat
    38041 ggtacgtagc ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct
    38101 gccggttcca gccccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct
    38161 gcgttgcgat gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc
    38221 cgtgttgctg ggcttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg
    38281 ccgcctggta gacacccatg cgattgccaa agattatcgt accgtgatgc tcaactaccg
    38341 taacggcatc aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc
    38401 ctcttaccgc tgctcgaaac tgtttgaagc ggtcggtcta cacgatgatg tagtgggcct
    38461 gtgcttccag ggggcggtca gccgcattgg tggggcaagc tttgaagact tccagcagga
    38521 tctgctgaat ctgtcgaaac gtgcctggct ggcgcgtaag cccatcagcc agggcggcct
    38581 gctgaaatac gtccacggcg gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct
    38641 gcaacaagcg gtacaaagcg gcgagtacag cgactatcag gaatacgcga agctggttaa
    38701 tgagcgtccg gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt
    38761 caacattgct gatgttgaac cggcaagcga actgtttaaa cgcttcgata ccgctgcgat
    38821 gtctatcggc gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaacagcat
    38881 cggcggtaac tcaaactccg gtgaaggcgg tgaagatccg gcgcgctacg gcaccaacaa
    38941 agtgtcgcgc atcaagcagg tggcttccgg tcgctttggg gttactccgg cgtatctggt
    39001 caatgccgac gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca
    39061 gttgccgggt gataaagtca ctccttacat cgccaaactg cgctattcgg tgcccggagt
    39121 gacgctgatc tccccgccgc cgcaccacga tatctactct atcgaggact tagcgcagct
    39181 cattttcgac ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga
    39241 accgggcgta ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat
    39301 cgcaggctat gacggtggca ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg
    39361 cccgtgggag ctggggcttg ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca
    39421 taagatccgt ttgcaggtcg atggcggcct gaaaaccggt gttgatatca tcaaagcggc
    39481 gattctcggc gcagaaagct tcggcttcgg cactggccca atggtcgcgc tcggctgtaa
    39541 atatctgcgt atttgccacc tgaacaactg cgcaacgggt gtggcaactc aggatgacaa
    39601 actgcgtaag aatcactatc acggcctgcc gttcaaggtg acgaattact ttgagtttat
    39661 cgcccgtgaa acccgcgagc tgatggcaca gctgggggta acgcggctgg tggatctgat
    39721 tggtcgcacc gacctgctga aagagctgga cggtttcacc gccaaacagc agaaactggc
    39781 gctgtcgaag ctgctggaga ccgccgaacc gcatccaggt aaggcactct actgcaccga
    39841 aaacaacccg ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc
    39901 gtttgtcgat gaacgccaga gcaaaacctt ctggttcgat atccgcaata ccgatcgctc
    39961 cgtgggcgcg tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc
    40021 cgatcctatc aaagcgtact tcaacggcac cgcaggccag agtttcggtg tatggaacgc
    40081 gggcggcgtg gaactgtacc tgaccgggga tgccaacgac tatgtcggta aaggcatggc
    40141 gggcggctta atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag
    40201 tattatcggc aacacctgcc tgtatggcgc gaccggtggt cgtctgtatg ccgcaggccg
    40261 cgcgggtgaa cgtttcggcg tgcggaactc cggtgctatc accgtggtag aaggcattgg
    40321 cgacaacggc tgtgaatata tgacgggcgg tattgtctgt attctgggta aaaccggcgt
    40381 taacttcggt gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt
    40441 ccgcaaacgc gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca
    40501 tgaagagcat ctgcgcggtc ttatcaccga gcatgttcag catactggct ctcagcgcgg
    40561 tgaagagatt ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa
    40621 gtccagtgat gtaaaagcac tgctgggtca tcgtagtcgt agcgcagcag agttgcgcgt
    40681 gcaggcgcag taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca
    40741 gcgcgttgat ccgccaaaga aaccgctgaa gatccgcaaa attgagtttg ttgaaattta
    40801 cgagccgttt tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa
    40861 cccatactgc gagtggaaat gtccggtaca caactacatc ccgaactggc tgaagctcgc
    40921 caacgagggg cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga
    40981 agtttgcgga cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga
    41041 tgagtttggc gcggtgacca tcggcaacat tgagcgttat atcaatgata aagcgttcga
    41101 aatgggctgg cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat
    41161 cggcgcaggc ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc
    41221 cgttgtcttc gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt
    41281 caagctggaa aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga
    41341 attcaaactc aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta
    41401 cgatgccgtc ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga
    41461 agacgccgat ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat
    41521 gggctttggt gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt
    41581 ccttggcggt ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa
    41641 gcacgttacc tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt
    41701 gaaaaacgcg cgggaagaag gcgtagagtt caaattcaac gtccagccac tggggattga
    41761 agtgaacggt aacggcaaag tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc
    41821 ggatgccaaa ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc
    41881 agatgcagtg atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca
    41941 cagcgtcgag ctggattcac aaggccgcat catcgccccg gaaggcagcg acaacgcctt
    42001 ccagaccagc aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt
    42061 ggtaaccgct attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt
    42121 ttaagctctg tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct
    42181 ttctggaaag cgcctcgcat aatctgaaga aaaccaatgt aatacgctca tctggattgt
    42241 taccggtaga tgcccggcgt atagctgatt ccattattca acgcagcgcc atataaaggg
    42301 acagataatg gaatcgctct cagagagaac ctcaacaggc taccagcaaa tccacgacgg
    42361 tattattcac ctggtcgata gcgcccggac ggaaacggta cgtagcgtta acgcgctgat
    42421 gaccgcgaca tactgggaaa ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc
    42481 aagggctgcg tatggtgcgc agctaatcaa gcgactatca aaggatttaa gtctaaggta
    42541 taagcgtggt ttctctgcaa aaaacttacg ccaaatgagg cttttttacc tcttttttca
    42601 acatgttgaa attcgccaga cagtgtctgg cgaattaaca ccattgggaa ttccgcagac
    42661 accatctgca gaattcccat cggcaaagat ttggcagacg ctgtctgcca aattgagagg
    42721 ctgtttgata tttccacagc ttttgtattg catctcctat tttcggtttg tatattcatg
    42781 caaataaacg aaggaaagtt gccgtgattt acattgaaca gcttgaatta atccataaaa
    42841 gtggtgatgt gttgtatcca gtaaaaataa ccagaaaatc atcaggaaaa actgccttcc
    42901 accttgttcc attcggctta aataagactc acgaccttct agaggtggaa gatgcttcag
    42961 aagcaattcg gctggtgatt gatgaacgtc attccattcg ctgcagtaca cttacagcaa
    43021 ctattacgaa taaaaagggc aagcgaatta agcggacagg tatatacagt atcaagggtg
    43081 ttaacataaa agaatacaac gtcaggtaat ggcacagagc ggtccgtcag ttcaggtttg
    43141 gctctgtgcc aaagatgggt caggtcacac ctgatctaat aaatttaagc cattaaatca
    43201 gccttaacct tcaccaccac ctttttaatc tcacccggct cgccaacaac gcatcccggc
    43261 ttatgcggct ctcctagcat aaacacggcg aacattcccg gtttcaggat gatggcttgc
    43321 tcgttttcaa tggtgctgca aagctggtaa tcatcctcat ggtggaactc ttcacactga
    43381 cgcgcagtgc ctgccatgcc aaacagaatc cgttcttcac cgtttaataa cagctggata
    43441 tcaatgtatt gctcgtgcag ctccgctttt ttctcaacgg gcgattgagt gttaaacgtc
    43501 atgacattca taaaaatatt gtcgccctgt aattcgtaac gacccggcgc tttttcttgc
    43561 ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac
    43621 ggtaatgacc gtacttcacc catcatcata atttttctcc ctgggccaac agcgctgccc
    43681 caagtaaacc tgcatcatgg cggtagtgcg ccgccagtaa atcaacatga aataccgctg
    43741 gctcctgcgc cagatacgtt tccaccaacg ccagataccc ttctgccaga ccaacgctgc
    43801 caccgaccac cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcca
    43861 gcacatgtgc ggagcggtga atcagttgct gcgcctgctc gtcgccctgc ccggcgcgtg
    43921 tgaaaatagt tctcgcatcc gcgcccatga gttcaccttg cgctgccgct gcaataccgc
    43981 gaccagaagc aatcgcttcc acgcaacctg tgcgtccaca gccgcagact gggccgtgtg
    44041 gatcggcaag cgtatgcccg atatgccccg ccagaccgcc agggccggta agcagtttac
    44101 cgccgctcac tacaccgccg ccgacgccag tggaaacggt gataaacacc atctcggtta
    44161 catcgccttc cagcgcctga tactctgccc atgctgcggc ctgcgcgtcg ttaatggcaa
    44221 tggtcggcaa attggtaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac
    44281 cgagattatg cggattcagc gccagcaaac tgccgtcgcg aataattccg gttgaagcga
    44341 tggcaacccg ctgcgcatga gcttgcaacg gagagactaa tgcggataag gcatcacgca
    44401 aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccgt
    44461 cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agtgtggtca
    44521 tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat
    44581 ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg
    44641 cgtgttgtaa cgcccttcgg caatcacccg acatccggct tcgctcaacg ttttcaccag
    44701 cgccagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat
    44761 aatttcggcg cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat
    44821 cgccagtaaa ccgtgatgat gtatacgtgc cagcagcgtt tcaacaggca ccggacgcgg
    44881 gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcgacatc
    44941 ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat
    45001 aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt cgatgcgaat
    45061 ggcaaccgcg cccgcctgct ctgccgctaa tgccatggct gcgacgattt cgggtttatc
    45121 gagcgggctg tcaggaatcg gctggcagga gacaatcagg ccaccgttag cagcgatttt
    45181 ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacatag
    45241 accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgct gccaaatggc
    45301 acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc
    45361 agccaacgct gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc
    45421 aggctgaacg agagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg
    45481 ccgatgattg gggccagtgc gccacccaat gcgccaacgt tgtaggtaaa gcccaggccc
    45541 gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc
    45601 ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg
    45661 ccaatcgcaa ataccggaat aatcagcagc tgcgaggcca gcaggctaca aacgtacgct
    45721 ttgcgggttc ccagccagtc accgaggaag cctcctacgc agcatcccac ggccgcgcca
    45781 aagccactaa agaacagcac attggcgaca gtatgcgggt tataagccag atcggttttc
    45841 agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc
    45901 accatcagca ttacgcccgt tggccagcgt ttgcctgtac tctgcaccat aaagctgata
    45961 aagattgcgg cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg
    46021 gcgaagcaga accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga
    46081 tgttcaccac ggtagagaat atccaccatt gtgcgtactg gtgctttacc tgcgtgtttc
    46141 tctttccagt cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc
    46201 aaaatgccga taaagaacag cgcacgccaa ccccagaccg gaaccaccag gctatagacc
    46261 tgagcggcaa cgacggcccc cacagagaag cctgaaatca aaaaaccact ggctttgtta
    46321 cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc
    46381 atccccatgc cgatgaccag acgagcgata aacatggtga tgtagcctgg cgcaaagccg
    46441 caggccagcg tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg
    46501 tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg
    46561 gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt
    46621 accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa
    46681 aatgcgcgcc attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta
    46741 ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtatgaa aattgtaggg
    46801 tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaaa caactcaccc
    46861 gcgctcttgc atcaactgct gggccagcgc cttcagttct ggcagatatt tttcatctac
    46921 cggtccaaac ggtttgcggc acagcggcac agaaacgaca tccatataat gcaggacagt
    46981 tttcaggccg cggaatacgc cagttttgat cagtaaatca atgactttgt tgcactcagt
    47041 ttgcagtttc tgcgcggtct ggatatcgcc ttctttcagc gccttcacga tcccctgata
    47101 tcgccagccc atgatgttgt aagtactgcc gataccacca tcagcgcctg ccagcaagcc
    47161 ggaagcgaag atttcgtcgt aaccgttata gagcaccaga tcaggatgtt cacgacggat
    47221 ctgttccatc tgatagagat cgccagaagt ctgtttcagc gcaccgacgc caggcaacgt
    47281 aaccagggtg ttgatctgat ccagggtcag ttttacccca ctcagggctg gaatgttgta
    47341 cacaaccatc ggcaaaccat ccgctgaatc aataattgcc cgatagtgat cgcagtgttc
    47401 ttcaaagctg aaaggatagt agaacggcgt gacggcggag acggcatcga agccataacg
    47461 tttagccgat gccgcaagtt gttggctttc ggcggtgctg acgcaaccga cgtgggcgat
    47521 gagtttaatc ttacctttcg cctcttcggc gacgatttcc agtacctgtt cacgctcgga
    47581 aaggctttgt acaaaggcct cgccggtcga accacccacg tataaaccgt cgatgccctg
    47641 ctgaatattg aactgaacca ggcgacgcag actcgcttta tccagtgctt gttgttggtc
    47701 aaaaggagtc aggagtgcag ccattacgcc acgtaaattc gttgccataa atacctctga
    47761 agtgatactt gtctgataaa cgatatacct ttatacctgt tataccagat caattaagca
    47821 acaccccata cagaaagctt ataatgcgat ctgcttcact aaagtggcat tatttctttt
    47881 tgttggtggt ctgaccgaaa gcgtgccagg tagcagagac gctgttgaga tgcgattgca
    47941 acgcacgatc ggcttcgtca ggatcatgac ggcggatcgc atcaacgatc gcaatatgct
    48001 gttgatagct aacgttgtta tgctcgtgca gagcctgatc agccacggtt ggacgtgcgg
    48061 cgatcagcca gtcgagcagg gcaacgtgga tcgccatgaa gattgggtta ccagggatct
    48121 ccgccagcac gcggtggaaa tcaacgtctg aacgaatgaa tgccgcgttg ttatccagcg
    48181 actgactgtt gatttccagt gcttttgcca gcaaatcgat ttgctcatcg gtggcatgtt
    48241 cagccgcata gcgcaccaga ctggattcaa agaacagacg taattgttcg aaatgggcaa
    48301 tcccaccggg atgagaaagg aaatctttcg ccatgccgga aaactcaccg atgatagtgt
    48361 ccgcagaagg acgcgagacg cgagcgcgtt cgccgttgtt tatttgcacc agacctttgc
    48421 gttttaacgc tgccagcgct tcgcgcacag aaggacgccc gacgttaaag aacgccatca
    48481 gttcgcgttc agacggtaat tgttcacctt cgccaaattc acgacggcgg atcatctgtt
    48541 ccagctcttc ttccaccatt tcggagagtt ttttacgcgc cagcgggcgg ctacgcaagt
    48601 tgcgaccaat tgcaggtgaa gaatcttcgg tttgcgaatc aaatgcgttc ataaggccca
    48661 ttctgtaagg tcagtgtgat taacatcatc agtgacatcc tatcacagga ttgaaagtag
    48721 gggaaaatgg caggtttttc tctttgtgcc tcatcattac cataattaac ggaataatta
    48781 actattgcga aaaattaatg taacgcagat aaacacatcc cgattgaatt attcataaga
    48841 ctattcacaa gcattatgaa tattatgaat gtgttcttac aaaataatca taagcgcata
    48901 ttttttaatg aaaaatcaca tcacctacaa ttaaaaacac gacatccgca ccataaatag
    48961 acttgcaaaa aatataacat cattgttttc aatctgccgt ttatgggatt gaccgttttc
    49021 ttttgacacg gagttcaaca atgttcggca taattatatc tgtcatcgta ttaattacga
    49081 tgggctattt gatcctgaaa aactacaaac ctcaggtggt gctggctgcc gcaggtgtct
    49141 tcctgatgat gtgcggagtc tggttagggt tcggtggtgt actcgatccc gccaaaagca
    49201 gcggctactt gatcgtcgac atttataatg aaatcctgcg catgctgtcc aaccgcattg
    49261 ccggattggg gctgtcgatt atggcggtgg gcggttatgc ccgctacatg gagcgcatag
    49321 gagccagtcg cgcgatggtg agcttgctaa gccgcccgtt aaaactcatt cgctcgccgt
    49381 atattattct gtcggcaact tacgtcatcg gccaaatcat ggcgcagttt attaccagcg
    49441 cctccggtct gggtatgttg ctgatggtca ccttatttcc gacgctggtg agtctgggag
    49501 taagtcgcct ctctgcggtg gcggttatcg caaccacgat gtccattgag tgggggattc
    49561 tggaaacgaa ctccattttt gcagcccagg tcgcgggaat gaaaattgcc acatacttct
    49621 tccactacca gcttccggtc gcctcttgcg tcattatctc ggtggcgatc tcccactttt
    49681 tcgtgcaacg cgcttttgac aaaaaagata aaaatatcaa tcacgaacag gcagagcaaa
    49741 aaactctcga taatgtcccg ccgctctatt acgccatttt acctgtgatg ccgttaatcc
    49801 tgatgctcgg ctcgctgttc ctcgcccacg tcgggctgat gaagtcagaa ctgcatctgg
    49861 tggtggtgat gttactaagt ttgacggtga cgatgtttgt tgagttcttc cgcaagcata
    49921 acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt acgcagtttg
    49981 ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta acgacgattg
    50041 gcactgttga tgcggttatc aggggtgcgg agcattctgg tctgggcggt attggcgtga
    50101 tgattattat ggcgctagtc attgccattt gtgccattgt gatgggctct ggcaatgcgc
    50161 cgtttatgtc atttgccagt cttattccga atatcgcagc cggactacat gtaccagcgg
    50221 ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcgcggtt tcgccgatta
    50281 ctgcggtggt gaagcggaca gcgatcccca tggcagtcgg tttcgtggtg aatatgattg
    50341 ccacaatcac gctattttat taagtcatta aaaagacaaa acaggccgcc tgggcctgtt
    50401 ttgtattact tcacaacacg taatgccggt cgaccaccgc gtggtggctg cggaggttca
    50461 tcgtcaggat gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg
    50521 gtttcgttgt ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc atcgtaggca
    50581 gcttcaggct caaacatcgt gcctgcgcca ttttcacggg cgtagatagc cagcacggca
    50641 gccagcggca cagaaacctg acgcggaatg ccaccaaagc gcgcgttaaa gcgcacctca
    50701 tcattcgcca gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg
    50761 tcacgcgcat attccatagg aacctgcacg ccagggagcg tcacatccac caccaggtgc
    50821 ggcgtgagct ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt
    50881 ggtgttagct gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg
    50941 cttcagttaa agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca
    51001 gctctttcgc acccgggccg ctgagctcga tacccagttg cggcagacgc cacagcagcg
    51061 gagcaagata gcaatcgacc aggctgaact catcgctcag gaagtacggc ttctgaccga
    51121 agaccggcgc aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag
    51181 aagctgaacc gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca
    51241 tgtacagacg gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga
    51301 aacgctcatc cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca
    51361 ccagggtcgg aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct
    51421 tttccacgtg ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat
    51481 ggctatagat gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga
    51541 cagccatgaa aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc
    51601 agtcagaagt tgtgttaccc aataaggaac gactctcttt gttcgaaaat caaacaaaaa
    51661 atgagcaata cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc
    51721 attgtggtga gtggatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt
    51781 gacttagacc cttattggcg atgtggtttt tgttttgcct gtctttcaga cggcagcaaa
    51841 aagcaacttc ccatttttta cgctgattca gaatttagcc ataaaaaaac ccgccgaagc
    51901 gggttttttc gtaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc
    51961 ggacgacgac gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga
    52021 gtaacgaagc cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg
    52081 cgggtgatac cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg
    52141 atgtacaggt ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg
    52201 cgggcagttt cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg
    52261 ttgcccggtt tgatgaaaac gcgagctgcg gaatttttgc ggcgaccagt gccgtagtat
    52321 tgattttcag ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc
    52381 gcgtggttgt gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc
    52441 gggccttttg gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca
    52501 atcatctctt caaagctcac ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac
    52561 actttgtcag tacgcttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg
    52621 taatcaccgg tatctacgtg cggagtgtat tccgctttgt gcttaccgcg taggcgacga
    52681 gccagttcag tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg
    52741 cgttttacgg tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag
    52801 ttacacgttg gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg
    52861 caaacctacc ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaaacttt
    52921 cttgtaacgt ggggtcgcag gattatagag aagtcggggt caaagatcga cccctttttg
    52981 tgatttgtga caggttttaa cccgccaaat gctcgcgctt cagatactct tcgctttgca
    53041 tctcttgcag acgtgacagg caacgctgga actcaaactt cagccgatcg ccctgataaa
    53101 tttcatacag cggcacttct gcactcacca ctaatttgac atggcgctcg taaaactcat
    53161 ccaccagcgc aataaagcgc cgcgcttcgc tctccatcaa ccgcgtcata actggtacat
    53221 caaacaacat gaccgtatga aagagacgtg agagcgcaat atagtcatgc tgactgcggg
    53281 cgtcgacgca cagcgtagta aaagagaccg ccagcgtctg gttctcgacg cccattgtcg
    53341 ctaatggccg atggttgatt tctaacgtcg gtgaattttc tcgtttcccc cccgccagcg
    53401 ccaaccatag tttatccatt tgcgcccggg tttcatcgtg gagtggcgaa agccacagat
    53461 gcgcctgagt gagtgtacgc agtcgataat caacaccagc gtccacgttc attacatcac
    53521 aatgctgttt aatggcatcg attgcaggca gaaaacgcgc acgttgcaag ccatttcgat
    53581 aaagttcgtc cggcggaata tttgacgtcg ctaccagggt aataccgcgg gcgaacaagg
    53641 ctttcatcag accgccaagt agcatggcat cggtaatatc agaaacaaaa aattcgtcaa
    53701 aacagagcac gtcagtttcg gctttaaagc gatcggcaat aatttccagc ggatcggtct
    53761 gcccctgtaa ggcagttagc tcttcatgca cccgcagcat aaaacggtga aagtgcaggc
    53821 gctgtttccg ctctcccggc aggctttgat agaaaaggtc catcagccag gttttcccgc
    53881 gtcctacacc gccccacata tataagccac gcactggcgt atgctttgtg tcttcgcgtt
    53941 taccccacag cttaccgacc cgcgccatta gcccactcgt cctgggggct ggtggcgtgc
    54001 tattgatgag ttcctgataa ataatttcca ggcggctgac ggcctctttt tgaacgtcgt
    54061 cgggttgatg gctgccttca ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt
    54121 gcatgatctt attgttattc cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa
    54181 aaaggccgtt ctacactacg cgatatgcag tcgggattcc acttctgtgg aattaacggt
    54241 tatagtggca taatcagccg caggcatgga gcctgaagcc aacaccctac ggaaacaaaa
    54301 gacaacggga gatgttcatg acctgggaat atgcgctaat tgggttagtc gtcggcatca
    54361 ttattggtgc tgtggccatg cgttttggta atcgtaaact acgccagcaa caggcgttgc
    54421 agtacgaact ggaaaagaat aaagctgaac tggacgagta tcgcgaagag ctggttagcc
    54481 actttgcccg cagcgcggaa ttactggata ccatggcgca cgactatcgc cagctgtatc
    54541 agcacatggc gaaaagctcc agcagcctgc tgccggaact gtctgctgaa gcaaacccgt
    54601 tccgtaatcg tctggcagag tctgaagcca gcaacgatca ggcaccggtg cagatgcctc
    54661 gcgactattc tgaaggcgca tccggcctgc tgcgtactgg cgcgaagcgc gactaattta
    54721 tttttcgggc gcagccattg cgccctcctc ttctctccct ccccgactat catttaatct
    54781 ggtgtctcat tgttagccgt ctgaaaattc aataacatca aactgttttg aatctctttt
    54841 cttatcattc aggtacgaga gcaggaataa tgaaaaaaca accccagctg ttgagtgcat
    54901 tagcgttaag tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg tcgattccag
    54961 gccaggttgc cgatcaggcc cctctcccca gtctggctcc aatgctggaa aaagtgcttc
    55021 cggcagtggt gagcgtacgg gtggaaggaa cggccagtca gggacagaaa atcccggaag
    55081 aattcaaaaa gttttttggt gatgatttac cggagcaacc tgcacaaccc ttcgaaggtt
    55141 taggctccgg tgtcatcatc aacgccagta aaggctatgt gctgaccaac aaccatgtga
    55201 ttaatcaggc acagaaaatc agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac
    55261 tgattggtag cgatgaccag agcgatatcg ccctgttaca aattcaaaac ccgagcaaat
    55321 taacgcaaat cgctattgcc gactccgata aattgcgcgt cggtgatttt gccgtagcgg
    55381 tcggtaaccc atttggcctt gggcaaaccg ccacctctgg cattgtttcc gcattaggac
    55441 gcagcgggtt gaatcttgaa ggtctggaaa actttatcca gacagatgct tccattaacc
    55501 gcggtaactc cggcggtgca ctgttaaacc ttaacggtga gttaattggc atcaacactg
    55561 caatccttgc gcctggcggc gggagcgtcg ggattggatt tgccatcccc agtaatatgg
    55621 cgcgaacact ggcgcagcag cttatcgact ttggtgaaat caaacgcggt ttgttaggca
    55681 tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg
    55741 gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcggc gaaagcgggc gtcaaagcgg
    55801 gcgatattat taccagcctc aacggcaaac cgctgaatag ctttgctgag ttgcgctctc
    55861 gtatcgcgac caccgagccg ggcacaaaag tgaaacttgg cctgctgcgt aacggcaaac
    55921 cactggaagt agaagtgacg ctcgatacca gcacctcttc gtcggccagc gctgaaatga
    55981 tcgcgccagc gctggaaggt gcaacgttga gcgatggtca gctaaaagat ggcggcaaag
    56041 gtattaagat cgatgaagtt gtcaaaggaa gcccagctgc tcaggctggc ttgcaaaaag
    56101 acgatgtgat cattggcgtc aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag
    56161 tgctggcggc aaaatcggcc atcatcgccc tgcaaattgt acgcggcaat gaaagcatct
    56221 atctgctgat gcgttaatgt cgtaaaccgg gcatcaggct tacgtgtgat gtccggttaa
    56281 ctcatggtat gctgctgccg ttcccttttt taatgacgcc tccatcatgt ttgtgaagct
    56341 cttacgttcc gttgcgattg gattaattgt cggcgctatt ctgctggttg ccatgccttc
    56401 gctgcgcagc cttaacccgc tttccactcc gcaatttgac agtaccgatg agacgcctgc
    56461 cagctataat ctggcggttc gccgcgccgc gccagcggtg gttaacgttt acaaccgtgg
    56521 tttgaacacc aactctcaca accagcttga gatccgcacc ctgggatccg gtgtaatcat
    56581 ggatcaacgc ggttatatca tcaccaataa acacgtcatc aacgacgccg atcagatcat
    56641 cgtcgcctta caggatggtc gtgtatttga agcattgctg gtgggatctg actctctaac
    56701 cgatctggcg gtacttaaaa ttaatgccac tggcggttta cctaccattc caatcaatgc
    56761 acgtcgcgta ccgcatattg gcgacgtggt actggcgatc ggtaacccgt acaacctcgg
    56821 gcagaccatt acccagggga ttattagtgc cacgggtcga attggtctga acccgaccgg
    56881 gcggcaaaac ttcctgcaaa ccgatgcttc cattaaccac ggtaactctg gcggcgcgct
    56941 ggtgaactcg ctgggcgaac tgatgggcat taacacgctg tcgtttgata agagtaacga
    57001 tggcgaaacg ccggaaggta tcggctttgc gattcctttc cagttagcaa ccaaaattat
    57061 ggataagctg atccgcgatg gtcgcgtgat ccgcggctac attggcattg gcggacgtga
    57121 gatcgcaccg ctgcacgcac agggcggtgg tatagatcaa ctgcaaggga tcgtggttaa
    57181 tgaagtgtca cctgacggcc cggcggcgaa tgcgggtatt caggtcaacg atctgattat
    57241 ttcggtggat aacaaaccgg ccatctctgc tctggagacg atggatcagg tggcggaaat
    57301 tcgccctggt tcggtgatcc cggttgtagt gatgcgtgat gataagcagt taacgctgca
    57361 ggtcaccatt caggaatatc cggcaaccaa ttaagtcgag tgctcaaaac aaaaaaccgg
    57421 agtctgtg
//
LOCUS       DAZOZY010000005        53207 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZOZY010000005 DAZOZY010000000
VERSION     DAZOZY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53207
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.005"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            75..359
                     /gene="pduA"
                     /locus_tag="ACHURQ_000247"
     CDS             75..359
                     /gene="pduA"
                     /locus_tag="ACHURQ_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR0822129.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            356..1168
                     /gene="pduB"
                     /locus_tag="ACHURQ_000248"
                     /pseudo
     CDS             356..1168
                     /gene="pduB"
                     /locus_tag="ACHURQ_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            1187..2851
                     /gene="pduC"
                     /locus_tag="ACHURQ_000249"
     CDS             1187..2851
                     /gene="pduC"
                     /locus_tag="ACHURQ_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR0822130.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2861..3529
                     /locus_tag="ACHURQ_000250"
     CDS             2861..3529
                     /locus_tag="ACHURQ_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR0822131.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3544..4062
                     /gene="pduE"
                     /locus_tag="ACHURQ_000251"
     CDS             3544..4062
                     /gene="pduE"
                     /locus_tag="ACHURQ_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR0822132.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4072..5904
                     /locus_tag="ACHURQ_000252"
     CDS             4072..5904
                     /locus_tag="ACHURQ_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR0822133.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5894..6244
                     /locus_tag="ACHURQ_000253"
     CDS             5894..6244
                     /locus_tag="ACHURQ_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR0822134.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHURQ_000254"
     CDS             6264..6539
                     /gene="pduJ"
                     /locus_tag="ACHURQ_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR0822135.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6563..6985
                     /locus_tag="ACHURQ_000255"
     CDS             6563..6985
                     /locus_tag="ACHURQ_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR0822136.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6988..7620
                     /locus_tag="ACHURQ_000256"
     CDS             6988..7620
                     /locus_tag="ACHURQ_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR0822137.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7617..8108
                     /gene="pduM"
                     /locus_tag="ACHURQ_000257"
     CDS             7617..8108
                     /gene="pduM"
                     /locus_tag="ACHURQ_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR0822138.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8112..8387
                     /gene="pduN"
                     /locus_tag="ACHURQ_000258"
     CDS             8112..8387
                     /gene="pduN"
                     /locus_tag="ACHURQ_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR0822139.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8399..9406
                     /gene="pduO"
                     /locus_tag="ACHURQ_000259"
     CDS             8399..9406
                     /gene="pduO"
                     /locus_tag="ACHURQ_000259"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR0822140.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9403..10788
                     /gene="pduP"
                     /locus_tag="ACHURQ_000260"
                     /pseudo
     CDS             9403..10788
                     /gene="pduP"
                     /locus_tag="ACHURQ_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10799..11911
                     /locus_tag="ACHURQ_000261"
     CDS             10799..11911
                     /locus_tag="ACHURQ_000261"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR0822141.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11908..13251
                     /locus_tag="ACHURQ_000262"
     CDS             11908..13251
                     /locus_tag="ACHURQ_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0822142.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13254..13808
                     /gene="pduT"
                     /locus_tag="ACHURQ_000263"
     CDS             13254..13808
                     /gene="pduT"
                     /locus_tag="ACHURQ_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR0822143.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13808..14158
                     /gene="pduU"
                     /locus_tag="ACHURQ_000264"
     CDS             13808..14158
                     /gene="pduU"
                     /locus_tag="ACHURQ_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR0822144.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14163..14606
                     /locus_tag="ACHURQ_000265"
     CDS             14163..14606
                     /locus_tag="ACHURQ_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR0822145.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14707..15036)
                     /locus_tag="ACHURQ_000266"
     CDS             complement(14707..15036)
                     /locus_tag="ACHURQ_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR0822146.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15208..16266)
                     /locus_tag="ACHURQ_000267"
     CDS             complement(15208..16266)
                     /locus_tag="ACHURQ_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0822147.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="ACHURQ_000268"
     CDS             complement(16464..16937)
                     /gene="sbmC"
                     /locus_tag="ACHURQ_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR0822148.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="ACHURQ_000269"
     CDS             complement(17056..18222)
                     /gene="dacD"
                     /locus_tag="ACHURQ_000269"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR0822149.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18431..19858
                     /gene="sbcB"
                     /locus_tag="ACHURQ_000270"
     CDS             18431..19858
                     /gene="sbcB"
                     /locus_tag="ACHURQ_000270"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR0822150.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19901..20128)
                     /gene="tsuB"
                     /locus_tag="ACHURQ_000271"
     CDS             complement(19901..20128)
                     /gene="tsuB"
                     /locus_tag="ACHURQ_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR0822151.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20142..21200)
                     /gene="tsuA"
                     /locus_tag="ACHURQ_000272"
     CDS             complement(20142..21200)
                     /gene="tsuA"
                     /locus_tag="ACHURQ_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR0822152.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21379..22737)
                     /gene="plaP"
                     /locus_tag="ACHURQ_000273"
     CDS             complement(21379..22737)
                     /gene="plaP"
                     /locus_tag="ACHURQ_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR0822153.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22727..22789)
                     /gene="yoeI"
                     /locus_tag="ACHURQ_000274"
     CDS             complement(22727..22789)
                     /gene="yoeI"
                     /locus_tag="ACHURQ_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR0822154.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(23004..23933)
                     /locus_tag="ACHURQ_000275"
     CDS             complement(23004..23933)
                     /locus_tag="ACHURQ_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0822155.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(23979..24803)
                     /locus_tag="ACHURQ_000276"
     CDS             complement(23979..24803)
                     /locus_tag="ACHURQ_000276"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0822156.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(24886..25140)
                     /gene="yoeB"
                     /locus_tag="ACHURQ_000277"
     CDS             complement(24886..25140)
                     /gene="yoeB"
                     /locus_tag="ACHURQ_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR0822157.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(25137..25388)
                     /gene="yefM"
                     /locus_tag="ACHURQ_000278"
     CDS             complement(25137..25388)
                     /gene="yefM"
                     /locus_tag="ACHURQ_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR0822158.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            25671..25721
                     /gene="hisL"
                     /locus_tag="ACHURQ_000279"
     CDS             25671..25721
                     /gene="hisL"
                     /locus_tag="ACHURQ_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR0822159.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    25697..25820
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            25867..26766
                     /gene="hisG"
                     /locus_tag="ACHURQ_000280"
     CDS             25867..26766
                     /gene="hisG"
                     /locus_tag="ACHURQ_000280"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR0822160.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            26772..28076
                     /gene="hisD"
                     /locus_tag="ACHURQ_000281"
     CDS             26772..28076
                     /gene="hisD"
                     /locus_tag="ACHURQ_000281"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR0822161.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            28073..29143
                     /gene="hisC"
                     /locus_tag="ACHURQ_000282"
     CDS             28073..29143
                     /gene="hisC"
                     /locus_tag="ACHURQ_000282"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR0822162.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            29143..30210
                     /gene="hisB"
                     /locus_tag="ACHURQ_000283"
     CDS             29143..30210
                     /gene="hisB"
                     /locus_tag="ACHURQ_000283"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR0822163.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            30210..30800
                     /gene="hisH"
                     /locus_tag="ACHURQ_000284"
     CDS             30210..30800
                     /gene="hisH"
                     /locus_tag="ACHURQ_000284"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR0822164.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            30800..31537
                     /gene="hisA"
                     /locus_tag="ACHURQ_000285"
     CDS             30800..31537
                     /gene="hisA"
                     /locus_tag="ACHURQ_000285"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR0822165.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            31519..32295
                     /gene="hisF"
                     /locus_tag="ACHURQ_000286"
     CDS             31519..32295
                     /gene="hisF"
                     /locus_tag="ACHURQ_000286"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR0822166.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            32289..32900
                     /gene="hisIE"
                     /locus_tag="ACHURQ_000287"
     CDS             32289..32900
                     /gene="hisIE"
                     /locus_tag="ACHURQ_000287"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR0822167.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(32997..33974)
                     /gene="wzzB"
                     /locus_tag="ACHURQ_000288"
     CDS             complement(32997..33974)
                     /gene="wzzB"
                     /locus_tag="ACHURQ_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR0822168.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(34120..35286)
                     /gene="ugd"
                     /locus_tag="ACHURQ_000289"
     CDS             complement(34120..35286)
                     /gene="ugd"
                     /locus_tag="ACHURQ_000289"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR0822169.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(35536..36942)
                     /gene="gndA"
                     /locus_tag="ACHURQ_000290"
     CDS             complement(35536..36942)
                     /gene="gndA"
                     /locus_tag="ACHURQ_000290"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR0822170.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(37106..38476)
                     /gene="cpsG"
                     /locus_tag="ACHURQ_000291"
                     /gene_synonym="manB"
     CDS             complement(37106..38476)
                     /gene="cpsG"
                     /locus_tag="ACHURQ_000291"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR0822171.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(38581..40017)
                     /gene="cpsB"
                     /locus_tag="ACHURQ_000292"
     CDS             complement(38581..40017)
                     /gene="cpsB"
                     /locus_tag="ACHURQ_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR0822172.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(40020..41243)
                     /gene="wcaI"
                     /locus_tag="ACHURQ_000293"
     CDS             complement(40020..41243)
                     /gene="wcaI"
                     /locus_tag="ACHURQ_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR0822173.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(41240..41719)
                     /gene="gmm"
                     /locus_tag="ACHURQ_000294"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(41240..41719)
                     /gene="gmm"
                     /locus_tag="ACHURQ_000294"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR0822174.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(41722..42687)
                     /gene="fcl"
                     /locus_tag="ACHURQ_000295"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(41722..42687)
                     /gene="fcl"
                     /locus_tag="ACHURQ_000295"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR0822175.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(42690..43811)
                     /gene="gmd"
                     /locus_tag="ACHURQ_000296"
     CDS             complement(42690..43811)
                     /gene="gmd"
                     /locus_tag="ACHURQ_000296"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR0822176.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(43837..44385)
                     /gene="wcaF"
                     /locus_tag="ACHURQ_000297"
     CDS             complement(43837..44385)
                     /gene="wcaF"
                     /locus_tag="ACHURQ_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR0822177.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(44401..45147)
                     /gene="wcaE"
                     /locus_tag="ACHURQ_000298"
     CDS             complement(44401..45147)
                     /gene="wcaE"
                     /locus_tag="ACHURQ_000298"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR0822178.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(45158..46375)
                     /gene="wcaD"
                     /locus_tag="ACHURQ_000299"
     CDS             complement(45158..46375)
                     /gene="wcaD"
                     /locus_tag="ACHURQ_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR0822179.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(46350..47567)
                     /gene="wcaC"
                     /locus_tag="ACHURQ_000300"
     CDS             complement(46350..47567)
                     /gene="wcaC"
                     /locus_tag="ACHURQ_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR0822180.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(47564..48052)
                     /gene="wcaB"
                     /locus_tag="ACHURQ_000301"
     CDS             complement(47564..48052)
                     /gene="wcaB"
                     /locus_tag="ACHURQ_000301"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR0822181.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(48055..48894)
                     /gene="wcaA"
                     /locus_tag="ACHURQ_000302"
     CDS             complement(48055..48894)
                     /gene="wcaA"
                     /locus_tag="ACHURQ_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR0822182.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(48987..51149)
                     /gene="wzc"
                     /locus_tag="ACHURQ_000303"
     CDS             complement(48987..51149)
                     /gene="wzc"
                     /locus_tag="ACHURQ_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR0822183.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(51152..51595)
                     /gene="wzb"
                     /locus_tag="ACHURQ_000304"
     CDS             complement(51152..51595)
                     /gene="wzb"
                     /locus_tag="ACHURQ_000304"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR0822184.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(51601..52740)
                     /gene="wza"
                     /locus_tag="ACHURQ_000305"
     CDS             complement(51601..52740)
                     /gene="wza"
                     /locus_tag="ACHURQ_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR0822185.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            53049..53198
                     /locus_tag="ACHURQ_000306"
     CDS             53049..53198
                     /locus_tag="ACHURQ_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822186.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
BASE COUNT        13214 a        13981 c        13283 g        12729 t
ORIGIN      
        1 cgattaagtc agccattctt tttcctcgct tttttttata gtcatatcgt caaaaaaatc
       61 caggcgaggt ctttatgcaa caagaagcgt taggaatggt agaaaccaaa ggcttaactg
      121 ccgccataga ggccgcagac gccatggtga agtcggcaaa tgttttgctg gtcggctacg
      181 aaaaaatagg ttccgggctg gtgaccgtca tcgttcgtgg tgatgtcggg gcggtaaaag
      241 cggcaacaga cgcgggtgcc gcagctgcgc ataacgtcgg cgaagtaaaa gccgtacacg
      301 ttattccccg cccacatacc gatgtcgaaa aaatcttacc aaagggaatt aacccatgag
      361 cagcaatgaa ctggtcgaac agatcatggc gcaggtcatc gctcgcgtag cgacgccaga
      421 gcatgaggtt ctcccgaaaa ccactcatca aatacgatag acggctatgg cagaaaaaag
      481 ctgcacgcta acagaatttg ttggcaccgc tattggcgac actgtaggtc tggtcattgc
      541 gaacgttgat agcgccctgt tggacgcgat gaaacttgaa aaacgctatc gttccattgg
      601 cattctgggt gcgcgtattg gcgcagggcc acacattatg gccgctgatg aagcggtaaa
      661 agccaccaac actgaagtta ttagcattga acttcctcgc gatactaaag gcggtgccgg
      721 acacggctca ctgatcctct taggtggcaa cgatgtttcc gacgtgaaac gcggtattga
      781 agtcgccctg aaagaactcg atcggacctt tggcgatgtc tatgccaacg aagccggaca
      841 tcttgaactg caatataccg cccgcgccag ctatgcactg gaaaaagcgt ttggtgcacc
      901 actgggacgg gcttgcggcg ttattgtcgg cgctccggct gctgtgggtg ttctgatggc
      961 cgacaccgcg ctgaagtccg ccaatgtgga tgttgtggca tacagctccc cagctcaagg
     1021 caccagctac agcaatgaag tcattctgat tatctcgggt gactccggcg cagtccgcca
     1081 ggcggtcatc tccgcacgcg aaatcggtaa aaccgtgctt tcgactctcg gcgatacacc
     1141 gaaaaacgat cacccttcct acatctgaat tctctgaggc agatttatga gatcgaaaag
     1201 atttgaagca ctggcaaaac gcccggtcaa ccaggacggt ttcgttaaag agtggattga
     1261 agaaggcttt attgcgatgg aaagcccgaa cgatcctaaa ccgtcaatca aaattgttaa
     1321 cggcgtcgtt accgaactgg atggaaaacc cgccagccag tttgacctga tcgaccactt
     1381 tatcgcccgc tacggcatca atctcgcgcg cgccgaagaa gtcatcgcga tggattcggt
     1441 aaaacttgcc aatatgttgt gcgatccaaa tgtcaaacgt agcgatatcg ttccccttac
     1501 cacagcaatg acgcctgcaa aaatcgtcga agtggtttcg cagatgaacg tggtagagat
     1561 gatgatggcg atgcagaaaa tgcgcgcccg tcgaacacca tcacaacagg ctcacgtcac
     1621 caacgtcaaa gataacccag tacaaattgc cgccgacgca gctgaaggcg catggcgcgg
     1681 gtttgatgaa caggaaacca ccgttgccgt tgctcgctat gcaccgttca acgccatcgc
     1741 cctgctggtc ggctcacagg tgggacgtcc gggcgtcttg acccagtgtt cactggaaga
     1801 agccaccgaa ctgaaactcg gtatgttagg ccacacctgt tacgccgaaa caatttctgt
     1861 ctatggtaca gaacctgttt tcaccgatgg tgatgatacc ccgtggtcaa aagggtttct
     1921 cgcgtcatcc tatgcttctc gtggcttaaa aatgcgcttt acttccggct ccggctctga
     1981 agtacagatg ggttacgcgg aaggcaaatc aatgctttac ctcgaggctc gttgcattta
     2041 catcaccaaa gccgcaggcg ttcagggtct gcagaatggt tcggtaagct gcatcggtgt
     2101 tccatcagct gtgccatcag gcatccgcgc agtattagcg gaaaacttaa tttgctcatc
     2161 gctggatctg gaatgcgcct ccagtaatga ccagaccttt acccactcgg atatgcgccg
     2221 taccgcacgt ttcctgatgc aatttctgcc aggcaccgac tttatctctt ccggtttttc
     2281 cgctgtgccg aactacgaca acatgtttgc tggttcaaac gaagatgctg aagacttcga
     2341 tgattacaac gtcattcagc gtgacctgaa ggttgatggt gggctgcgac cagtacgcga
     2401 agaagatgtt atcgctatcc gcaataaagc cgctcgcgct ttacaggctg tctttgccgg
     2461 tatgggacta ccgcctatta ccgatgaaga agtcgaagcg gcaacctatg cccacggttc
     2521 aaaagatatg cctgagcgca atattgtgga agacatcaag ttcgcccagg agattatcaa
     2581 taaaaaccgg aacagcctgg aagtggtgaa agccttggcg caaggcggtt ttaccgatgt
     2641 cgcccaggac atgctcaaca tgcaaaaagc aaaactgacc ggcgattatc tccatacctc
     2701 agccatcatc gtcgatgacg gacaagtgct ctctgcggtc aatgacgtca atgattacgc
     2761 cggaccggct acaggttacc gcctgcaagg ggaacgctgg gaagagatca aaaatattcc
     2821 cggtgcactt gatcccaacg aaattgacta agggtgagaa atggaaatta acgaaaaatt
     2881 actgcgtcag attatcgaag acgtgctggc agaaatgcaa cccagcgaca agtccgtttc
     2941 atttcgcgca cctgtctctg caactgtccc ttccgcgcca gataccggaa attttctgac
     3001 cgaaattggc gaagcccaac aaggcactca gcaagatgaa gtcattattg ctgtaggccc
     3061 ggcgttcggc ctggcgcaaa ccgtcaatat catcggtatt ccgcataaaa acatcttgcg
     3121 cgaagtgatt gccggtatcg aagaagaagg cattaaagcc cgtgtaattc gctgctttaa
     3181 atcgtccgat gtcgcattcg tagcagtcga aggcgatcgt cttagtggtt caggtattgc
     3241 tatcggtatt cagtcgaaag gcacaacggt tatccaccag cagggacttc ctcccttgtc
     3301 taacctggaa cttttcccac aagcgccttt gttgacgctg gatacttatc gtcagattgg
     3361 caaaaacgcc gcgcgttatg cgaagcgcga gtctccacaa ccggtgccga ccctcaacga
     3421 tcaaatggcg cggccgaaat accaggccaa atctgccatt ttgcatatta aagagacgaa
     3481 gtacgtggtc actggcaaaa aaccgcaaga actgcgcgtg acgttttaac aaaggaaacc
     3541 ttcatgaata ccgatgcaat tgagtcgatg gtcagggacg tgttgaaccg catgaacagt
     3601 ttacaggatg cagctcctgt ttctgcggtc cccaacgcat caatactgag cgccaaagtg
     3661 acggattacc cgctggcaaa taaacatccg gaatgggtta aaacagcgac caataaaacg
     3721 ctcgacgatt tcactctgga aaatgtgcta agcgataacg tcaccgccct ggatatgcgc
     3781 attacccctg aaacattgcg tattcaggct gcgattgcca gagatgctgg tcgcgatcgt
     3841 ctggcgatga attttgaacg cgccgctgag cttacctcag taccagatga tcgcatcctc
     3901 gaaatctaca atgcgttacg cccttatcgt tcaactaagc aagaacttat tgccattgct
     3961 gacgatcttg aacagcgtta tcaggccaaa atttgtgctg catttgtccg tgaagctgcg
     4021 gaactttatg tcgagcgcaa aaaactgaaa ggcgacgatt aaggacgaac tatgcgatat
     4081 atagcaggca ttgatatcgg caactcatca acagaggttg cgctggcaac gctcagtgca
     4141 actggagagt tgtcgttcgt gagtagcgcc ctggaggaaa ccaccggaat caaaggtacg
     4201 ttgcgtaacg tccatggtat tcaggaggcg ctcgcacagg caacgaaaaa agtcggcatc
     4261 aatgttagcg atatttcgct tattcgtatc aacgaagcaa ctccggtcat tggtgatgtg
     4321 gcgatggaaa ccatcactga aactatcatc accgaatcaa ccatgatcgg tcacaacccg
     4381 aaaactccgg gcggagtggg gctgggcgta gggttcacca tcactccgca agagttatta
     4441 acacgcccgg cggacacacc ttacatcctg gtggtgtcat cggcatttga ttttgccgat
     4501 atcgcgacga tgataaacgc ctccgtacgc gccggatatc agctgactgg cgttattctg
     4561 caacgcgacg atggcgtact ggtcaataac cgcctggaaa ttcctttacc tattgtggat
     4621 gaagtcctct acatcgatcg tattccgctc ggcatgctgg cagcaattga agttgccgtt
     4681 cccgggaaag ttatcgaaac cctgtctaac ccgtatggga ttgccaccgt attcgccctc
     4741 aatgctgaag agactaaaaa tattgttcct gtcgcgcggg cgctaatcgg caaccgttca
     4801 gcagtggtag tgaaaacgcc ctctggcgac gttaaagccc gttcgatacc cgccggaaat
     4861 atcgaactgc tttctgccgg gcgcacaacg cgcgttgatg tagctgctgg tgccgatgcc
     4921 attatgaaag cggtcggtga gtgcccaaaa ctggaaaacg tgacgggaga accaggcacc
     4981 aacatcggcg gaatgctgga acatgttcgc cagaccatgg cggaactgac caacaaaccc
     5041 agcaacgaaa tttttattca ggatttgctg gcgatagata cctctgtccc cgtcagcgta
     5101 accggtggtc tggcaggtga attttcactg gaacaggctg tgggtattgc ctctatggtg
     5161 aaatccgacc gtctgcaaat ggcgatgatc gccagtgaaa ttaagcaaaa actgcatgtt
     5221 gatgttcagg tcggaggcgc agaagcagaa gccgccattc agggggcctt gactacccct
     5281 ggaaccacac gtccactggc aattctggac ttaggtgcag gctctaccga tgcctccatc
     5341 atcaatcaga gtggggaaat cgttgccact caccttgcgg gagcgggcga catggtaacg
     5401 atgattatcg ctcgtgagct gggcttaaat gatcgttatc tggcagaaga aatcaaaaag
     5461 tacccgttag caaaagtaga aagcctgttc cacttacgcc atgaagacgg tagcgttcag
     5521 ttcttcccta ctcccctatc cccccatgta tttgcccgcg tctgtgtagt gaaacccgac
     5581 gaactggtgc ctattcccgg tgatttgacg ctggaaaaag tacgcgctgt tcgtcgcagt
     5641 gcgaaagagc gtgtcttcgt aaccaatgcc ctgcgcgcgc tacgtcaggt aagccctgcc
     5701 ggaaatattc gcgacattcc gttcgtcgtt ctggtgggtg gttcttcgct ggatttcgaa
     5761 gtaccgcaat tggtcaccga cgcgctggca cattatcgac tggtcgccgg gcgggggaat
     5821 atccgtggta gcgaaggtcc gcgcaacgcg gtagctactg ggctactcat cgcctggcac
     5881 aaggagtcga ttcatggaaa gtaaagagcg tgcaccagcc atcgttgtca tggagatcgg
     5941 tgactgtatc actacatggg atgaggtgct tcttggcatt gaagaggaag gtattccctt
     6001 tcgcattcaa cacattccgt caggtgaagt tatcgatagc gcctggctgg ctgctcgtca
     6061 atctcccctg ttggttggca ttgcctgcga ccgggaagaa ctgattgtgc attacaaaaa
     6121 cttaccggca tcagcgccac tgtttacgct gacgtatcaa caagataacc atacccggcg
     6181 cagcatcggc actaacgctg cccgcctggt aaaaggaatt cccttccggg aattccactc
     6241 ataatccaca ggagaaacgc tgtatgaaca acgcactagg actggttgaa acaaaagggt
     6301 tagttggcgc aattgaagct gcagatgcca tggttaaatc cgccaacgtg cagctggttg
     6361 gctatgaaaa gattggttcg ggtctgatta cggtgatggt ccgcggtgat gttggcgcag
     6421 taaaggccgc cgttgatgcg gggagcgctg cggcaagcgc cgttggcgaa gtgaaatcct
     6481 gccatgtcat cccgcgccca cacagtgatg ttgaggctat tttacctaaa tcggcataac
     6541 cccatttgct aaggagcaca gcgtgaagca atcactggga ttacttgaag ccagtggtct
     6601 ggctctcgcc atcagttgcg cagacgctat ggcaaaagcc gcgtccatca cgctggttgg
     6661 ccttgagaag accaatggct caggctggac gctcatcaaa atcaccggtg acgtcgcctc
     6721 ggttaatgcg gcgatcgcga cgggagccag tttcgccgga cagcgaaatg gccttgtcgc
     6781 acacaaagtg attgcccgcc cgggagacgg cattctgccc cacacaactg aagcattgac
     6841 cgtgacggtt gaaacaagca aaacaccagc aacaaccgtc acctcagaga ttatcagttg
     6901 taatttgtgt cttgacccgg catgtcagcg gcaaaaaggt gagccccgct cactttgcct
     6961 gtacgccaat aagcgagggg agtagcagtg gataaacaaa ccttgcagga aacagtgagc
     7021 cagattctgg atgagatgcg cgagcgccct atcccactgg gcgtttccag tcgccatata
     7081 catctttcat cagcggatta tttgcgtctt tttcctgaac aaccgattag cgagaaaaag
     7141 ccgttaatgc aacctggcca atacgctgct gaacaggttg ttacgctgga gggaccaaaa
     7201 ggcaaattgc acaacgttcg cttgttgggg ccgctacgca atgcaagcca ggtggaaatt
     7261 tcccggacgg acgccagaac gttagggatc accgccccgc tacgcatgtc aggcgatctc
     7321 agcggcactc cggggattcg tttgataagc ccatttgcgg aactggagat cacatctggc
     7381 gtcattgtcg cgcaacggca tattcatatg tctccgctgg atgccctgat ttatggcgtt
     7441 acgcatggcg ataaagtggc ggttgccatt gaaggtgatg agcgacgact gatattcgaa
     7501 aacgtcgctg ttcgcgtctc tccggatatg cgtcttgaaa tgcatattga taccgatgag
     7561 gccaacgccg caggcgctga tagccctgac gtttttgcca cactggtgtc agtgccatga
     7621 ataacatgct gatgcaacgt attgttgacg aagtggtttt tcgccttaaa caacgtgcgg
     7681 gaaaaacact cgtgctgact gtttttcaat tgcgagacgc cagcgttcaa gagagcgttc
     7741 atcagtatgc gtcattacaa atccgatatg tcgatctgcc gctgctgcga caacttgcgg
     7801 agaacgaaac gtcagatcga gcatctatcc agatacacga agcgttagcc tggggaatgc
     7861 acattcaatt gtcgttgcag cgtcatttcc tgaacgccat cgaattgaag actctcgccc
     7921 gtctaccgct aagctggtgc gacgaacaag ggcagccgat ctaccttcat cataatcgac
     7981 ttttaagtta tgccgatatc gcacagttaa gctcgggaat actggtgtta cagcggaaat
     8041 gttgtgtgac cgcactggct cgtgaagcgg caattacccg aaatattcag ttaatcaggc
     8101 aggagtaacc tatgcatctg gctcgagtca ctggcgcggt agtatccacg caaaaatcac
     8161 cttctctgat cgggaaaaaa ctgctgctgg ttcgccgggt cagtgctgac ggcgaactgc
     8221 ctctcacgcc attaaccggc gacgaagttg ccgttgattc agtcggcgca ggcgttggtg
     8281 agttggtact cctcagcagt ggttccagcg ccagacatgt gttttcaggc cctaacgagg
     8341 ccatcgacct ggcagtggtc ggcattgtcg atacgctttc gcgctaagga gctaaaagat
     8401 ggcaatttat acccgaacag gtgattcagg aagtacgtct ttatttaccg gacagcgcgt
     8461 cagcaaaact catctacgtg tcgaaaccta tggcacgctg gatgaactga acgcgacact
     8521 cagtctgtgt tattgcgcca ccgccatcga aagccatcgc atcttactgg aagccattca
     8581 acaacagata ttttggttta gcgccgaact cgccagtgaa agcgaacagc cgtcagcgca
     8641 acagcgttac atcggtacgg aagaaattgc cgcgttggaa aacgcgatcg atagtgcaat
     8701 gaacgccgtc cccccagttc acagcttcat tctgcctggt cgatgtgaag ccgcaagccg
     8761 catgcatttc gcccgcacgg tggctcgacg ggctgaaaga cggctggttg aactgacaac
     8821 ggaaaccacc gtccggaatg ttttgctgca ctatatcaac cgactttccg attgcctgta
     8881 tgccctcgcc cgcgtggaag acaacgttgc tcatcaaaac ctgatgattc aggaaatcac
     8941 aaaacgttat cacgaagcca accacacacc agcattgaag gaacgcacaa tgccgctcac
     9001 ttttcaggat cttcaccagc tcattcgtag tgcggcaatg cgcgcggatg aactgcatat
     9061 tcccgttgtc atcagcattg ttgatgccaa cggaacagaa agtgttacct ggcggatgcc
     9121 tgatgccctg ttggtcagca gtgaactggc accgaaaaaa gcctggaccg ccgtggcgat
     9181 gaaaacggca acccacaagc ttgctgatac cgtgcaaccc gacgcgccgc tttacgggct
     9241 cgaaagccat atgcaaggaa aagtcgtcac ttttggtggc ggcttccccc tctggcgtga
     9301 cggaaaattg cttggcgggc ttggcatcag cggcggtagc gttgaacaag acatggatat
     9361 tgcccaaagc gcaatggcgg caattaacgt gggagtaaac caatgaataa ctctgaatta
     9421 gaaacactga tccgcaatat tctgcgtgag caacttgtcc ccgctacacc acgaacgcaa
     9481 agaaacgcta ttttccagac tgtcgatgaa gcggtctgcg cagcacacta ggccttttta
     9541 cgctttcaac agtgcccgct taaaacccgt agcgccatta tcagcgccat tcgcgaagaa
     9601 ttaatgcccc gccttaccga actggcagaa gaaagcgcca aagaaacggg gatgggaaat
     9661 aaagaagata agtacctaaa aaacaaagcg gcactcgata acacgccagg aattgaagat
     9721 ttaaccacca cagcactaac cggtgatggt ggcatggttt tgtttgagta ctctccgttt
     9781 ggggtgattg gctcggtcac gccaagcacc aaccccacgg aaaccatcat taacaacagc
     9841 atcagcatgc tggcagcagg taatagcgtc tacttcagtc cacacccggg tgccaaaaac
     9901 gtttcgttaa aactgattac cacaatcgaa gacatcgcgt ttcgctgtag tggcattcat
     9961 aacttgatcg ttaccgtatc tgaaccgacc tttgctgcca ccgaacagat gatggcgcac
    10021 cctaaaatca gcgttctggc gattaccggc ggtccgggga tcgtcgccat gggaatgaaa
    10081 agtggcaaga aagtgattgg tgccggagcg ggtaaccctc cgtgcatcgt cgatgaaacg
    10141 gcggatattg tgaaagccgc cgaggacatc atcaacggcg cttcatttga ttacaacctg
    10201 ccgtgtattg ctgaaaaaag cctgattgtc gtggcatcag tggcagatta tttaattcag
    10261 cagatgcaaa gttttggcgc acttcttctt aactatgagc aaactgagaa attgcgtgca
    10321 atttgcctgc cagacggtag cgccaacaaa aaactggttg ggaaaagccc atcagccttg
    10381 ctggaagcgg caggcctccc tttgcccgca aaagcgccgc gcctactgat tgctgttgtc
    10441 gacgccgatg atagttgggt tacttgtgag caactaatgc caatgctgcc cattgtgaag
    10501 gtcaaggatt ttgacagcgc tctaaccctg gcgctgaaag tggaagacgg cttgcaccac
    10561 acggcaatta tgcactctca gaacgtctca cgactcaacc ttgccgcgcg cgttatgcaa
    10621 acctcaatat ttgtcaaaaa cggcccctcc tgggcgggta ttggcgtcgg cggcgaaggc
    10681 tttacgacct ttaccattgc aacaccgact ggagagggta caacgtcagc acggactttt
    10741 gcccgctcac gacgctgcgt gttaaccagc ggcttttcaa ttcgctagag aaacggaaat
    10801 gaatactttt tcactgcaga cgcgtttata cagcggacca ggaagtctca aggtacttag
    10861 ccgtttctct catagacaca tctggattat ttgcgatgcc tttctggcac gctcaccact
    10921 tattgacacg ttgcatcagg cgctgcccga cagtaatcgt ctcagtattt ttagcgaaat
    10981 aacgcccgcc ccaactatcc agaccgtggt gaaaggcatt gcgcaaatgc agactttacg
    11041 cccggatgta gttatcggtt ttggcggcgg ttctgccctg gacgccgcaa aagcaattgt
    11101 ctggttcggt cgccagtgtg gcattgaaat tgaaacctgc gtagccattc ctaccaccag
    11161 cggtatcggt tccgaagtga ccagtgcttg tgtcatcagc gatcctgaga aagggattaa
    11221 atatcccctt ttcgataatg cactttatcc ggatatcgcg atccttgatc catctttggt
    11281 cgtcagcgtg ccaccggcga taacggccaa taccgggatg gatgttttaa cccatgcgct
    11341 tgaagcttat gtttctcctc gcgccagcga cttcacggat gcactggtcg aaaaagccgt
    11401 gcaaattgtt tttcaatacc tgcccactgc agtgaaaaaa ggcgattgtc ttgccacccg
    11461 tggcaaaatg cataatgcat cgacgcttgc aggaatggcc tttagccagg ccggactcgg
    11521 tttaaaccat gcaattgctc atcaactcgg cggacagttt catctgccgc atggactggc
    11581 gaatgcgctc ctgctcacgg cagttatccg ctttaatgct ggcgatccgc gcacagccaa
    11641 acgctatgcc cggctggcga aaacgtgcca tttatgcccg gacaacgcca atgacaccgc
    11701 cagcctgaat gcacttattc aacatattga gcagcttaat acaacctgta cgctgccgac
    11761 tcttgccaat gcgctgaaag aaaaaaaagc agaatggtca atacgcatac cagatatggt
    11821 tcaggcggcg ctggcggatg caacgttgcg taccaacccg cgtgcagctg atgcctccgc
    11881 gattgcagaa ctgctcgagg agttgttatg aacgtcggat taatctcctg cgacgaccag
    11941 accatccgcg agcgagtaca agcagcaggt gtcgtgggtg ctggcggcgc aggttttcca
    12001 acccatatta agctacaagc gcgtgctgac actttcctgg tgaatgcggc ggaatgcgaa
    12061 cccatgttga aagtagatca acagttaatg gcgcagcaag ccagccgcct gcttcgcggt
    12121 gttcactatg cgatgaaagc caccggggcc agtagcggaa tcatcgcgct gaaagaaaaa
    12181 tatcaacggg caataaacgc actcacacca ctgttaccac cggatatccg cctgcatatt
    12241 ttgcccgacg tttaccccgc aggagacgaa gtgctgacca tctggatggc aactggtcgc
    12301 cgcgttccac ccgctgcatt acccgttagc gtcggtgtgg tggtcaataa tgttcaaacc
    12361 gtacttaata ttgccagagc cgtcgagcaa cagtatccag tgacgcgccg cacactcacc
    12421 gttaatggtg cagtggcgaa gccaataacg gtaaccgtcc ccatcggcat gtctttacgt
    12481 gaagtcctgg cgctggcggg cggcgcaaca gtggatgctc ctggttttat caatggcggg
    12541 ccgatgatgg gaagtttgat cacatcgctc gacacaccag tcagcaaaac cactggcggc
    12601 ttgctggttt tacccaactc tcattcactg atccaacgtc gaatgcagaa tgaccgcagc
    12661 gtacttgccg tcgcgaaaac tgtttgtgag caatgccgct tgtgtaccga tctctgccca
    12721 cgtcatttaa tcggtcacga actggcaccc catttattag tacgtgcggt taactaccaa
    12781 cagctcgcca cgccgcaact gttactgaca gcgctcacct gttcagagtg caacgtctgc
    12841 gccagtgtcg cctgtccggt aggaatttca ccaatgcgca taaaccgcct gcttaagcag
    12901 gaattacgcg cacaaaatct ccgctacgaa ggtgccttaa atcctgcaga tcccatggca
    12961 aattatcgac tgattcccgt aaaacggctg gtaaccagac ttggcttaac cccgtggtat
    13021 caggatgcgc cattgtctga acaggtgcct cagcccgaaa aagtgacgtt attgttacgc
    13081 cagcacatcg gtgccagcgc aattgcgtgt gtacaaaaag gcgatcgcgt cgttcatggg
    13141 caatgtgtag gtcaaattcc acacggcaca ttaggtgccc ccattcatgc cagtattgat
    13201 ggcatggtca gcgatgtgac agaaaacgcg attacgcttg tgagaggtta accatgtcga
    13261 aagcggtagg tattttagaa cttagcagta tcgcgaaagg gatggaaacc ggcgacgcca
    13321 tgctgaaaag cgccaacgtt gagttactgg tcagcaaaac gctatgcccc ggaaaatttc
    13381 tgctgatgct cggtggcgat gtgggcgcgg tgcaacaggc catcaacagc ggagcgtccc
    13441 ttgctggtga aatgctggtc gacagtcttg tcctgccgaa tattcatccc agcctccttt
    13501 ctgcaatcag cggacttaac catgttgaac agcaccgggc tgtcggcgta gtcgaaacct
    13561 ggagtgtcgc cgcctgcatt gacgctgccg attgtgctgt caaagctgca aatgtcattc
    13621 ttgttcgcgt acatatggct tttggcattg gcggtaaatg ctacatggtg gttgctggcg
    13681 atatttcaga tgttaataac gcggtctctg ccgccagcgt gcgcgcgggg gaaaaaggtt
    13741 tgttggtcta tcggtcggtg attgcccgtc cacatgaagc catgtggcgt cagatggtgg
    13801 agggataatg acaactcagc acacaactga acggatgatt caggaatatg tcccaggtaa
    13861 gcaggtcacg ctggcacatg tcatcgccaa tccagggaag gatctgttta agaaattggg
    13921 tttaccagac accatttccg ctattggtat tctgacaatt acccccagcg aagcatctat
    13981 catcgcatgc gatatcgcca caaaatcagg cgctgtagag atcggttttc ttgatcgttt
    14041 taccggtgcc gtagtgctga ctggtgatgt ttctgcgatc gaatatgcac ttagccaggt
    14101 tacccgtacg ctgggtgaaa tgatgcgctt taccgcctgc cccattacgc ggacctgacg
    14161 ttatgcaacg tatgatgctc atcggcccca gccagtgcgg aaaaacgtca ctcacgcaat
    14221 gtctgcgtgg cgaaaatatt cattatcaga aaacgcaggc aatcgtctgg tccccgacca
    14281 ccattgatac accaggcgaa tacctcgaaa accgcagcct ctacagtgca ctgttggtca
    14341 gtgcctgcga agcggacatt atcgcgctgg tgcttaatgc ggacgcaccg tggtcacctt
    14401 tttcgccagg ctttacctcg tcaatgaatc gcccggtcat tggagtagta accaaagcag
    14461 atttagccaa catggaacaa atatctttgg tgaaatgttg gttacgggaa gctggagcgc
    14521 acaacgtgtt agtaaccagt gcggttaata accatggagt tgcagaactt ttagccttgc
    14581 tgcatacaga agaaggctgt cgctaatttc gacaggttat cagatgggaa tgatgacggt
    14641 gatacacaga aaagaatcaa tgagcaatga gggttgccgg gcaaccctca ttgaataaaa
    14701 caggaattac ttcgcttcgc cgtttttcat ttcgcccata gctttcagct ttttggagat
    14761 atcgcggcgt tctttggaga gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc
    14821 atagtcacct ttcatgctgg cgatgatgcc ctggattgct tcaacgctca tcccaggctt
    14881 gatgtaatcg ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac ggatcttttt
    14941 ctcaacgtcc tgaatttcac gttgcagttt gttcttacga cggaacagac gaacaaattc
    15001 cagtacgtcc tggaatgaag gcttggtagt ttccattttt atacccctga taatgtgaga
    15061 gtcagattcg tttaagcaac cgctattcgt tagggccaac attactcata gctgccgcag
    15121 atgacaatgc ttttatccct tttcactatc atacccttta tccttgctga atcgaagcag
    15181 cagcaagatg attctgaagt tcaggaatta tttgcgcaag gcccggcaaa tcagattcga
    15241 tagcaactca agctgatgcg ccgtttccat gttcagccac acataaccat agattggtgt
    15301 ttccactacc ttcaggtcat ggtggttatt gagcaactga cgcagctctt ccacagcatc
    15361 gttcaatttt tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac
    15421 aaagctcagc agtatttgct gcatcatgtg ctgggtatca cgaagttttt gcgcgttcaa
    15481 taacacgaaa tggctggggc gcgtggccca gtacgcattg atttgcaact ccagcataca
    15541 aaccagattg cggttaatgg tctggatacc ttcatatatc gattttggaa tacgggtttc
    15601 tttgctggcg ggcgcaatca gcccacgcat tttcacggca tcggtcagga gtttttgtag
    15661 atggctttcc agacgtgggc gttcgagtaa gttcggtgag aatgcagatt gatagacccg
    15721 attatactcg gtcagacttt tcgccagttg aatgcgccag tggatgaacg cccgttgtgg
    15781 ccagataccg gtaaacaaca ttgccagtaa agagccgagg atcacatcgc cgcttcgcca
    15841 taacgccgta tcaatttcac ctgtcgggga accaacaaca attgccagcg tcaccccaat
    15901 caataatcct tgatacggtt tcttgcccag cgccagccaa ccgcaaagaa acatcgccgc
    15961 cgcgcaccag actaacatca gcggtaacga aattaactcc agttgcagag cgataagacc
    16021 taaaatcgaa cccaacaccg taccgccaat acgttcgaag gcacgaggga caacgttacc
    16081 ccagaacgag attggcccca taatcaccac catggtgacc agcggccagg tgctttccgg
    16141 gatagtaaac aggcggataa tgagaaaagt gagcaggaat gccagcgcga cccgagtacc
    16201 atgcactatg cggtaatggc ggtatacccg gatttcaaac gggcttaatg acttatcggc
    16261 acgcacgcag aaactccgta atgaagatta aaagcgctca taactcatct gtcggccaga
    16321 aggcgcgcct ctgaattatc cgacggacaa accgggaaca gtgtaatttc ccggcaggag
    16381 cgaaaaaatt gaaaggcgca tcttactctt ttcgctttca aaaaaagagt ggtcatcgcg
    16441 ttaacacacc gccctgagat gaattagtga tgttttggct gcaccgcaac atacatttcg
    16501 atatcccagt acccatcttc cgcgccattg ttcaaataaa cctcgaagca gggctttggt
    16561 aacatttcat aagcactgtc ctgcaagagg ctattaaaga actgatacca gggtttagca
    16621 aaatcatcac cgactacacg agccaccgcc accgcatact gaccacctga aatttctgtc
    16681 agaatgacgc cctcactgtt ttcgggaagc gtaaagttat tcggtaccgt cacaacggtg
    16741 tcgcagcgta atttttcggc gggtgtttca tctggattgt cgtaatagac agcaacccac
    16801 tccttcggca caatattttt gttatctacc cacatcatca actgctcaaa gcctttcttt
    16861 accgtctgtt cccacgggcc aacgagatgg aaacctgcaa cggtacgttt ctcttcctgc
    16921 gtaatctcgt agttcatgac gcctccatta acactgtttt tatatacagc atagttgcaa
    16981 tattaaaacc acaaggaatg agtgttgatt atgcgagcag actcgcactc ctgccagtct
    17041 gctgcaaaag aaaggtcagg ccttatggtg gaaataatca ctcaggcggg aaaacatgcc
    17101 gccttccccg acagattcca gggtaaccag cggccagtgc gccacctgtt tatcacggtc
    17161 gtaaagttca atttccccta cccgctgatg ggcgctaatt ggcgcggtga gctctttacc
    17221 atcaagggta tatttggctt tgatatgtgg aatttcggct ttcggtagca ccatccagaa
    17281 ctcttgttcc gttcctaggt cgatattttc tttatcgcca taccagatgc gttccgttcc
    17341 gacctttttc ccacggtgca aaatttgcac cgtagtaaag ttttgttgcc cccaacgcag
    17401 taattttctt gcctcttcct cacgaccttt tgcactgtca gcccccatta ccactgcaat
    17461 gagacgacgc tgcccatcta cagccgaagc aatgagatta aacccggcac cagaagtatg
    17521 acccgttttc aggccgtcaa cattcatggt tttatcccac aacaacccgt tacggttttg
    17581 ctgggtgata ccgttccagg tgagactttt ctcactgtac atatgataaa actcgggctc
    17641 gccgtggatg atagcgcgag aaagcacagc taaatcataa gccgagctat gctggccagg
    17701 tgcatccaga ccatgcactg tttcaaaatg cgtatccttg agatgcagct tctcggcata
    17761 gttgttcatc atttcaacaa actgccgttg cccaccggca atatagtcag ccagagcaac
    17821 acaagcgtca tttccggaat ccacaattaa accacggctt aaatcacgta ccgatacgcg
    17881 atcgccctct ttcaaaaaca tcagtgaaga accgacaaac accggattat ctttcgccca
    17941 cgcatcgcgc cccacggtga caatatcgtc tggcgtaatg cgatgactat cgatagcgcg
    18001 atccacgaca taacccgtca tcagctttgt caggctggcg ggattgcgct gttgatgctc
    18061 attacccgcg gtgaggatct gaccggtggt gtaatccatc aatacccagg acccggcatg
    18121 aatctctgga ggctgaggtg aaaaaggaat gttttccgcc gcaaaaccag acgataagtt
    18181 aaaaacgaac aaagaagcag caataataag acggcgtttc aacagcaaac cctcaggagt
    18241 ttcaaatagc tgttcttttt acggaaatac ttatgaactg gctggaataa agtgcaagaa
    18301 aatgtgacta ccctctcatt tttatctgac atgatctgtt gccactcgct gccaaattgt
    18361 ggcgctaaag ctgattagca cggtgatatt tgatactctg gcagacagca gaaataacgg
    18421 atttaaccta atgatgaatg acggtaagca acaatctacc tttttgtttc acgattacga
    18481 aacctttggc acgcaccccg cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga
    18541 tagcgaattc aatgtcatcg gcgaacccga agtcttttac tgcaagcccg ctgatgacta
    18601 tttaccccag ccaggagccg tattaattac cggtattacc ccgcaggaag cacgggcgaa
    18661 aggagaaaac gaagccgcgt ttgccgcccg tattcactcg ctttttaccg taccgaagac
    18721 ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca acgtttttta
    18781 tcgtaatttc tacgatcctt acgcctggag ctggcagcat gataactcgc gctgggattt
    18841 gctggatgtt atgcgtgcct gttatgccct gcgcccggaa ggaataaact ggcctgaaaa
    18901 tgatgacggt ctaccgagct ttcgccttga gcatttaacc aaagcgaatg gaattgaaca
    18961 tagcaacgcc cacgatgcga tggctgatgt gtacgccact attgcgatgg cgaaactggt
    19021 aaaaacgcgt cagccacgtc tgtttgatta tctctttacc catcgtaata aacacaaact
    19081 gatggcgttg attgatgttc cgcagatgaa acccctggtt cacgtttccg gaatgtttgg
    19141 ggcatggcgc ggcaatacca gctgggtggc accgctggcg tggcatccag aaaatcgcaa
    19201 tgccgtaatt atggtggatt tggcaggaga catttcgcca ttactggaac tggatagcga
    19261 cacattgcgc gagcgtttat atactgcaaa agccgatctt ggcgataacg ccgccgttcc
    19321 ggttaagctg gtgcatatca ataaatgtcc ggtgctggcc caggcgaata cgctacgccc
    19381 ggaagatgcc gaccgactgg gaattaatcg tcagcattgc ctcgataacc tgaaaattct
    19441 gcgtgaaaat ccgcaagtgc gcgaaaaagt ggtggcgata ttcgcggaag ccgaaccgtt
    19501 tacgccttca gataacgtgg atgcacagct ttataacggc tttttcagtg acgcagatcg
    19561 tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac tggatatcac
    19621 ttttgtcgat aaacggattg aaaagctgtt gttcaattat cgggcacgca acttcccggg
    19681 gacgctggat tatgccgagc agcaacgctg gctggagcac cgccgccagg tcttcacgcc
    19741 cgagttttta caaggttatg cagatgaatt gcagatgctg gcacaacaat atgccgataa
    19801 caaagagaaa gtggcgctgt taaaagcgct ttggcagtac gcggaagaga tcgtctaatt
    19861 tgaagcctcc gccgctggta cggcggaggt caatgttgag tcaggctttt tgaacggtga
    19921 tactccacgc tgcatcgcca atctgctgat aatcggtgat ggcatgccct tcttctgccg
    19981 cccactgtgg gattgcttca gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc
    20041 cgctggccat ttctgccagt gctgcttttg cttctatgag tggaaacgga cagacctgcg
    20101 tcaccacatc cagcttttta atcgccatct ttacacctta cttaatttgc cgcagcagtt
    20161 gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc aggacgcagt ccagactccg
    20221 agaatcataa atacaaggcc aatccagccc tgccaggtca tcattgcagt cataaccagt
    20281 ccattaccga taaagcaacc acctgcaata ctggcaccga agcccatcag tacaccacct
    20341 aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct
    20401 ttggctgcaa taaatgaccc cacgaagatc cctaacacca ggaaaacgcc ccagttaatg
    20461 tatttcacgt cacccgcgac cagaaattgc agaatattgg ccgttgggga agtgattcct
    20521 aacccaaaca tgcgtccggt tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg
    20581 atgagtacag cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatccca
    20641 gttcggcgcg gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc
    20701 acccagagcg ttatcaccag caaaacggca accaacggcc aaacagacaa attaaaagtc
    20761 tcagcaatag agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca
    20821 tgtggagaac gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg
    20881 atcaatccct cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt
    20941 cccagcccga agatataccc acctataaca gtaccaagcc acgggaacgc ctcggcttcg
    21001 taagtcagta ggcccgcctg aattaacgca aaaaccccca cgctttgtac cgaaattgca
    21061 atcagcaagg cgtaaaacat gcgattattt ttcgcgatat acatatcgcg aaaaccacct
    21121 gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg
    21181 cttaatatca ttgaaaacat aaattaatta accagatgaa tgttaatgag gaaattattc
    21241 atgactggtg gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttattaa
    21301 tgaggatttc gataaaaaaa aataaaaaaa ccggagccaa atattcattc gactccggtt
    21361 gattattaga aaagatgatt atgcaacgtc ttcgtactgc ggaactgggt tacggaagct
    21421 cttggtgacg caagccaggt aaatcagacc gatagcagcc caaatcagac ccagaaccat
    21481 tgagctttct tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag
    21541 gaacagatac tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg
    21601 cgagatgacc gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt taatcagcgc
    21661 tgtagccatt accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat
    21721 gttcatcgcc ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac
    21781 gccgtcacga cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga
    21841 cgccagtacg gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac
    21901 gtacagcatg atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa
    21961 gtacagctgc aggaagtaag ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag
    22021 gaaaatcgca cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat
    22081 gccgtcaaag ccggtaaagg aaaagcacag gattgtcgcc ccggtaatca tcggaataac
    22141 atgcgcatcg ccagaccaga acggacgagt gctcgccagc gtaccagcgc cttcaccttc
    22201 aaatacgcca taaacaacca tgcccagaat caccgcgatc agcactacct gcaacacgac
    22261 gattacagtg ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcataaa
    22321 ggccaccagc gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata
    22381 gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca
    22441 acccaccata aagccgacag tcgggctaat ggatttctgg gcgtaagtat atgcagagcc
    22501 agcagaagga tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat
    22561 caatgcgaac gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa
    22621 agtatcaaac agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa
    22681 cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt
    22741 cattctcctt tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt
    22801 cctcagcgac aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc
    22861 ttggtatgaa tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt
    22921 agtcattttt tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag
    22981 aagcttgtca taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa
    23041 acaccggcga tcgcactatt tgctaaaatt tcatcccgcc accagccgct ggcaagccct
    23101 gcggtctgtt cgttccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc
    23161 ttctcgatta acgcgccact atcgagaaaa cgttgcacca gataacgggg taaatagccg
    23221 cagccgaggc cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct
    23281 tcttgttcgt cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt gtcccccacc
    23341 acaatcgccc gatatcgctt aatgatacga cggtttaacg gctcttcttc ctgggctaac
    23401 ggatgatgag gcgcgaccgc aaagacttgt tccagatcgc ccaaccgcga gaaaccaaac
    23461 tcactggaag atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc
    23521 agggcatccc aggaaccacc gagtacgcca ttgataaact tcaggcgcgt cacgctatga
    23581 tgttgataaa aagcttcaat cagtggcgca agcagagaaa aaggaaacgt gtcgtccacg
    23641 ccaatcacca gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttct
    23701 cgcacggtat gcagaacttc ccgccccttc tctaataaca tttttccggt acgggtgaat
    23761 ttagcgcggt gaccgctacg atcaagtaat tgaatattaa ggtcgctttc cagcttgtga
    23821 acggtgtaac tcagagcgga cggcgtcttg tagagtttag ccgacgccgc cgcaaaactg
    23881 ccttcttttt ctaaggcatc gaggatcatc aacacatcca gcagttgttt catacacgcc
    23941 cccttgcagt gcatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc
    24001 ggatcgggat actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc
    24061 ttacctttgc cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg
    24121 gccatctgcg gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg
    24181 tgtccgcctt taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg
    24241 actaaattaa caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga
    24301 tgacgtcccg gtccgaccag ccccgcaaga cgcagaatat cgaccgaagt accgggtaaa
    24361 ttgtgcagcc agtcttcgag ttcttctaat actcgtccac tgttggtcac cggattacgc
    24421 ggggtggttt ctttcaccgt gccttgcgcg tcgccataga cagacgttga gctggtaaaa
    24481 ataatgcgag gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa
    24541 tagaactcat cgccggggcc gctacgacgt gccggaagcg taatgaccag cgcatcggca
    24601 tccatcaggg catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga
    24661 tagctatcaa tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg
    24721 gtgacttgcc agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac
    24781 ccgacaattg cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta
    24841 cgctagcgta tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg
    24901 acatgctgca atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt
    24961 aatgcgtcgg gaccagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc
    25021 ttcaaatggc gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt
    25081 atctgtttcc tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac
    25141 tcaataatgt ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
    25201 ctccgggcgt tagcggggga gcgcagtaga taagccgtct cttccagcga gttgtattct
    25261 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga
    25321 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
    25381 gtacgcatgt caatctcctc ttttgtacag tttattgtac aatgatgggc gttaattaac
    25441 tatttattaa ttagtttgta gatcaaggta ttgtcagtga gacgaaaatc caggcttcgc
    25501 tatttttggt gccgtcagct tagaggacag tcctcttagc cccctccttt ccccgctcat
    25561 tcattaaaca aatccattgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
    25621 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagactttt atgacacgcg
    25681 ttcaatttaa acaccaccat catcaccatc atcctgacta gtctttcagg cgatgtgtgc
    25741 tggaagacat tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca
    25801 ccttccgggg gcttttttat tgcgcggttg ataacggttc agacaggttt aaagaggaat
    25861 aacaaaatga cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt
    25921 gatgactcac gcgaattact ggcgcgctgt ggcattaaaa ttaatcttca cacccagcgc
    25981 ctgatcgcga tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgacgacatt
    26041 cccggtctgg taatggatgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa
    26101 gaagagctgc ttaaccgccg cgcccagggt gaagatccgc gctactttac cttgcgtcgt
    26161 ctggatttcg gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggc
    26221 ccgctctcct taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat
    26281 ctcgaccaga aaggcatctc ttttaaatcc tgcttactga acggttctgt tgaagtcgcc
    26341 ccgcgtgccg gactggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa
    26401 gcaaacggcc tgcgcgaagt cgaagttatc taccgctcga aagcctgcct tatccagcgc
    26461 gatggtgaaa tggaagaatc caaacagcag ctgatcgaca aactgctgac ccgtattcag
    26521 ggtgtgatcc aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgtctg
    26581 gatgaagtca tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt
    26641 gaccaacaac gcgtagcgat gcacatggtc agtagcgaaa ccctgttctg ggaaacgatg
    26701 gaaaaactga aagcgctggg tgccagttca attctggtcc tgccgattga gaagatgatg
    26761 gagtgatcgc catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac
    26821 aacgccagct gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcactgtta
    26881 acgatattct cgataacgtg aaagcacgcg gtgatgacgc cctgcgggaa tacagcgcga
    26941 agtttgataa aaccacggtt aacgcgctga aggtgtctgc tgaggagatt gccgccgcta
    27001 gcgaacgcct gagcgacgag ctaaaacagg cgatggcggt ggcagtaaaa aatattgaaa
    27061 ccttccacac tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcca ggcgtgcgtt
    27121 gccagcaggt cacgcgcccg gtcgcttcag ttgggttgta tattcctggc ggctccgccc
    27181 cgctcttctc aacggtatta atgctggcga ctccggcgcg tattgcgggc tgtaaaaaag
    27241 tggtgctgtg ctcaccgccg ccgattgccg atgagatcct ttatgcggcg cagctgtgcg
    27301 gtgtgaagga cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta
    27361 cggaatctgt gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag
    27421 cgaaacgtca ggtgagccag cgtctggacg gtgcggctat cgatatgccc gcaggcccgt
    27481 cggaagtgtt ggtcattgcc gacagcggcg caacgccgga tttcgtggct tctgatttgc
    27541 tttctcaggc tgaacacggc ccggactcac aggtgatttt actgacgcct gacgctgata
    27601 tggcgcatca agttgccgaa gccgtcgaac gccagttagc agaactgccg cgtgccgaaa
    27661 ccgcccgcca ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg
    27721 tagagatctc caaccagtac ggcccggagc acctgatcat ccagacccgc aacgcccgcg
    27781 atctggtcga tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat
    27841 cggcaggtga ttacgcctcc ggcaccaacc acgttctgcc gacttacggt tacaccgcca
    27901 cctgttccag cctcgggctg gcagatttcc agaagcgtat gaccgtgcag gaactgtcga
    27961 aagaaggttt ctccgcgctg gcttcgacca ttgaaacatt ggccgccgcc gagcgcctga
    28021 ccgcccataa aaatgccgtt actttgcgtg ttaacgccct taaggagcaa gcatgagcac
    28081 cgtgactatt accgatttag cgcgtgaaaa cgtccgcaac ctgacgccgt atcagtcggc
    28141 gcgtcgtctg ggcggtaatg gcgacgtctg gctgaacgcc aacgaatacc ccactgccgt
    28201 ggagtttcag cttactcagc aaacgctcaa ccgctacccg gaatgtcagc cgaaagcggt
    28261 gattgaaaat tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagtcgtgg
    28321 cgcggacgaa ggtattgaac tgctgattcg cgctttttgc gaaccgggta aagacgccat
    28381 cctctactgc ccgccaacgt acggcatgta cagcgtcagc gccgaaacga ttggcgtcga
    28441 gtgccgcaca gtgccgacgc aggacaactg gcaactggac ttacagggca tttccgacaa
    28501 gctggacggc gtaaaagtgg tctatgtttg cagccccaac aacccgacag ggcaactgat
    28561 caatccgcag gattttcgca ctctgctgga gctaacccgc ggaaaagcca ttgtggttgc
    28621 cgatgaagcc tatatcgagt tttgcccgca ggcatcgctg gctggctggc tgacggaata
    28681 tccgcacctg gctattttgc gcacactgtc gaaagctttt gctctggcgg gccttcgttg
    28741 cggatttacg ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcgcccccta
    28801 cccgctctcg acgccggttg ccgacattgc ggcccaggcg ttaagcccgc agggaatcgt
    28861 cgctatgcgc gaacgagtga cgcaaattat tgcagaacgc gaatacctga ttgccgcact
    28921 gaaagagatc ccctgcgtag agcaggtttt cgactccgca accaactaca ttctggcgcg
    28981 ctttaaagcc tccagcgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga
    29041 tcagaataaa caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga
    29101 aagccagcgc gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt
    29161 tttatcgatc gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt
    29221 tttgacaaac tcgcctttga accaggcgtg atcccggaac tgctgaagct gcaaaaagcg
    29281 ggctacaaac tggtaatgat caccaatcag gatggtctgg gaacacaaag tttcccgcag
    29341 gcggatttcg atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtacag
    29401 tttgatgaag tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg
    29461 aaagtaaaac tggtggaacg ttatctggct gagcaagcga tggatcgcgc taacagttat
    29521 gtgattggcg atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta
    29581 cgctacgacc gcgaaatcct gaactggcca atgattggcg agcagctcac taaacgagac
    29641 cgttacgctc acgtagtgcg caacaccaaa gagacacaga ttgacgttca ggtgtggctg
    29701 gatcgcgaag gtggcagcaa gattaacacc ggtgttggct tctttgatca catgctggat
    29761 cagatcgcca cccacggtgg tttccgtatg gaaatcaacg tcaaaggcga cctctatatc
    29821 gacgatcacc acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaatggct
    29881 cttggcgaca aacgcggtat ttgccgcttt ggttttgtgc taccgatgga cgaatgcctt
    29941 gcccgctgcg cgctggatat ctctggtcgc ccgcacctgg aatacaaagc cgagtttacc
    30001 taccagcgcg tgggcgatct cagcaccgag atgatcgagc acttcttccg ttcgctctca
    30061 tacaccatgg gcgtgacgct acacctgaaa accaaaggta aaaacgatca ccaccgtgta
    30121 gagagcctgt tcaaagcctt tggtcgcacc ctgcgccaag ccatccgcgt ggaaggcgac
    30181 accctgccct cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat accggctgcg
    30241 ccaatctgaa ctcggtgaag tcagccattg cgcgccacgg ttatgaacca aaagtcagcc
    30301 gtgacccgga cgtggtgttg ctggccgata aactattttt acccggcgtt ggcactgcgc
    30361 aagcagcgat ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc
    30421 aaccggtact gggcatctgc ttagggatgc aactgctggg tcggcgcagc gaagagagca
    30481 acggcgtcga cttgctgggc atcattgacg aagacgtgcc gaaaatgacc gactttggtc
    30541 tgccgctgcc gcatatgggc tggaatcgcg tttacccgca ggcaggcaac cgcctgtttc
    30601 aggggattga agacggcgcg tacttttact ttgttcacag ttacgcaatg ccggtcaatc
    30661 cgtggaccat tgcccaatgt aattacggcg aaccgttcac cgcggcggta caaaaagata
    30721 acttctacgg cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga
    30781 aaaacttcct ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt
    30841 gcgtctccat cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg
    30901 cttacaggat tacgccgcgc agggtgccga agtgctgcat ctggtggatc tgaccggggc
    30961 aaaagatccg gcaaaacgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt
    31021 tccggtgcag gttggcggcg gtgtgcgtag cgaagaagac gtggcggcgt tactggaagc
    31081 gggcgttgcg cgcgtggtgg ttggctccac cgcggtgaaa tcaccagaaa gggtgaaagg
    31141 ctggtttgaa cgtttcggtg ccgatgcctt agtgctggcg ctggatgtcc gtattgacga
    31201 gcaaggcaac aagcaggtgg cggtcagcgg ctggcaagag aactcgggcg tttcactgga
    31261 acaactggtg gaaacctatc tgcctgtcgg cctgaaacat gtgctgtgta ccgatatctc
    31321 gcgcgacggc acgctggcag gctctaacgt ctctttatat gaagaagtgt gcgccagata
    31381 cccgcaggtg gcatttcagt cctccggcgg tattggcgac attaacgatg ttgctgccct
    31441 gcgaggcact ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac
    31501 cgtgaaggag gccatcgcat gctggcaaaa cgcataatcc catgtctcga cgttcgtgat
    31561 ggtcaggtgg tgaaaggcgt acagtttcgc aaccatgaaa ttattggcga tattgtaccg
    31621 ctggcaaaac gctacgccga agaaggtgca gacgaactgg tgttctacga tatcaccgct
    31681 tccagcgatg gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac
    31741 attccgttct gcgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc
    31801 tttggcgcgg ataaaatttc catcaactcc cctgcgctgg cagacccaac cttaattact
    31861 cgcctggcgg atcgctttgg cgtgcaatgc attgtggtcg gcattgatac ctggtacgac
    31921 ggcgaaaccg gtaaatatca tgtgaatcaa tataccggcg atgaaagccg cacccgcgtc
    31981 actcagtggg aaacactcga ctgggtacag gaagtgcaaa aacgtggagc cggagaaatc
    32041 gtcctcaaca tgatgaatca ggacggcgtg cgtaacggtt acgatctcga acaactgaaa
    32101 aaagtgcgtg aagtttgcca cgtcccgcta attgcctccg gtggcgcggg caccatggaa
    32161 cacttcctcg aagccttccg cgatgccgac gttgacggcg cgctggctgc ttccgtattc
    32221 cacaaacaaa taatcaatat tggtgaatta aaagcgtacc tggcaacaca gggcgtggag
    32281 atcaggatat gttaacagaa caacaacgcc gcgaactgga ctgggaaaaa accgacggac
    32341 taatgccggt aattgtgcaa cacgcggtat ccggcgaagt gttaatgctg ggctatatga
    32401 acccggaagc cttagacaaa accatcgaaa gcggcaaagt caccttcttc tcgcgcacta
    32461 aacagcgact gtggaccaaa ggcgaaacgt cgggcaattt cctcaacgta gtaaatattg
    32521 cgccggactg cgacaacgac acgctactgg tactggcgaa tcctatcgga ccaacctgcc
    32581 acaaaggcac cagcagctgc ttcggcgaca ccgctcacca atggctgttc ctgtatcaac
    32641 tggaacaact gctcgccgaa cgcaaatctg ccgacccgga aacctcctat accgccaaac
    32701 tgtatgccag cggcaccaaa cgcattgcgc agaaagtggg cgaagaaggc gtagaaaccg
    32761 cactggcagc gacggtacat gaccgctttg agctgaccaa cgaggcatcg gacttgatgt
    32821 atcacctgct ggtgctgttg caggatcagg atctggattt aacgacggta attgagaacc
    32881 tgcgtaaacg gcatcagtga gttgcagggt aagcggatgc gatgttgttg tcgcatccgg
    32941 caaaaaaaaa gggcaaggtg tcaccaccct gcccgttttc tttaaagctg caaagattac
    33001 ttcgcgttat aattacgcag agcgttacgc cccagcacaa tccccgcgcc aaccatgccg
    33061 cccagcagca ccgccagaat caaggtaatt gcctttttcg ggctatcgcg acgaataggt
    33121 aacgtcggtt tcatcacata acggtaagca tgaatatcaa gatcatcaac ctttaagttt
    33181 tcaatatcca acaggttttg acgtgtctga tagtagtttg gtgagaacac caacggacgg
    33241 gtcgcctcat gcttaatcat cgactccagc gcttcgctcc ccaaaaggaa caacgtatct
    33301 tgtgtcacat cttcagtctg ctgaacctgt ggctttgcca cctgcgcctg attcgcatac
    33361 tgcaacgctt cctgaatctg acggatacgc agatctttct gctcctgcgc gactacttcc
    33421 tgcgttctta aagagtcctg caagtttttc cgtcccagag caatgttgtc tttgagatcc
    33481 ttttctagct cttgattcac tttatcatca acttgctgaa tgtattgggc caacttcatt
    33541 tgtgctccct ctgcagtttg cccaacataa gaaacagtca atggtaattg ctggttctta
    33601 acagaaggtt cgatggtaag tttttctggt tcttcctgat tatccagcgt ttctgctaat
    33661 gcagagaagg cagaactgaa gcgaccaatt aacgtctcct gtaaatccga tactttcggt
    33721 gcagcctgac catagataac attcatggca ttgttatagc cagcaatttg ccccacgtcg
    33781 ggctgagtga taattgctgt tgacgtccat ttctccttcg ctactgccaa atatccaata
    33841 gccagggcaa tagccacaat gacggaaatg atgattgtca tcttgccacg ccacaactgc
    33901 actagtaaat caatcaaatc aatctgttcc gggtcatggt tttgcccaga aacattatta
    33961 ttttctactc tcatcattac cctaactgac ggaaatttcc aagtccgcat agtttagcga
    34021 taatcctagt gaatgctata ggataagtga taaaaataat gaattattgg ggaggtaaca
    34081 aaagacaatc agggcgtaaa tagccctgat aacaagatgt tagtcgctgc caaagagatc
    34141 gcgggtgtat accttatctg ccacatcttt aagctcttct gccattcggt tagagataat
    34201 gacgtcggct tgttgtttga agatggcgag atcacgttcc agccgagagt tgaagaatga
    34261 gtcttctttc atcactggct cgtagatgat cacttcaacg cctttcgcct tgatacgctt
    34321 cataatcccc tgaatagaag acgcacggaa gttatctgaa ccgctcttca taatcagacg
    34381 ataaataccc accacttgcg gtttgcgtga caaaatggca tcggcaataa aatccttacg
    34441 cgtgcggttt gcatcgacaa ttgccgagat caggttattc ggcacagact gatagttcgc
    34501 cagtaactgc ttggtatctt tcggcaagca ataaccacca taaccaaacg acgggttgtt
    34561 gtaatggttg ccaatacgcg gatcgagaca aacgccttcg ataatctggc gagtattcag
    34621 acctaaactt tctgcgtagc tatccagttc gttaaagtat gccacgcgca tcgctaggta
    34681 ggtgtttgca aaaagtttaa tcgcttctgc ttcggtcgaa tcggtgaaca gggtcgggat
    34741 attttgctta atcgcgcctt cctgtaacaa cgcagcaaaa cgttctgcgc gttctgaacg
    34801 ctcaccgatg acaatacgtg aaggatggag attatcgtaa agggctttac cttcacggag
    34861 aaattccgga gagaaaataa tattttcagt acgatatttc ttatgcatcg cttcggtaaa
    34921 accaacggga accgttgatt tgatgaccat aaccgcataa ggatttatct caactacgtc
    34981 tttaattact gattctacac tggatgtatt gaaataatta gttttaggat catagtcggt
    35041 tggagtggcg atgatgacat aatcagcatc ccggtaggct tcatttttat ctaatgtggc
    35101 attaaagtgt attttatctg attgcaaaaa ctgctgaatt tccttatcaa caataggaga
    35161 tatccgatca ttcagcatag caacgcgtga cggtaaaata tctaatgcca caacctcatg
    35221 attttgtgcg attagaagcc cgtttgacaa gcctacatag ccagtaccgg aaatggtgat
    35281 tttcatttca tgctctcaga attaacttaa ctgtgaatca tgatgttttt agcatcctga
    35341 taagagctaa atgttttaac gctacgttat tagctggtca acgctacgaa aaacaattca
    35401 gattttcttt ataagaatag ctgatattta ttaagttaat attaagcagt gaaatttagt
    35461 ctataagata aatggcaaaa aaaaagcccg gtgcaatata cgccgggcct caattttact
    35521 gttggttaaa tcagattaat ccagccattc agtatggaac acaccttctt tatcaatgcg
    35581 cttataagta tgtgcaccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac
    35641 agcggcacgg tagctatcgt aataggcaac cgcagcggcg aaggtcggaa ccgggatacc
    35701 gttctgtact gcataagcaa cgacatcacg cagcgcctgc tggtagtcat cggcaatttg
    35761 cttgaagtac ggagccagca gcaggttagc gatctgcgga ttttcggcat atgcatcggt
    35821 gattttctgc aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc
    35881 gccgtagttc agatcccagt tgtattcttc agacgcagca cgcagctgag agaagccctg
    35941 agcgtaagaa acgattttgc ccagatacag cgcacggcga actttttcga tgaactcagc
    36001 cttgtcgcct gctgactgag cttgcggacc agagagaact ttagatgcgg caacgcgctg
    36061 atctttcaga gaagagatat aacgtgcaaa cacagactcg gtaatcagcg acagcggttc
    36121 tccgagatcc agcgcgctct ggctggtcca tttaccggta cctttgttag ccgcttcatc
    36181 caggatcaca tcaaccaggt agttaccgtc ttcatctttt ttagtgaaga tatctttggt
    36241 gatgtcgatc aggtagctgc tcagttcacc gttattccac tcggtaaagg tctgtgccag
    36301 ttcttcgtta gagagattca ggccgccttt caggagagaa taggcttcag caatcagttg
    36361 catatcaccg tattcaatac cgttgtgaac catcttcaca taatggcctg cgccatcagc
    36421 accgatatag gtaacgcacg gttcgccatc ttcagcaacg gcggcgattt tggtcaggat
    36481 cggcgcaacc agttcatagg cttctttctg gccaccaggc atgatggacg gccctttcag
    36541 cgccccctct tctccgccgg aaacaccggt accgatgaag ttaaagcctt cggcagaaag
    36601 ctcacggtta cgacgaatgg tgtcctggaa gaaggtgtta ccaccatcga tgatgatgtc
    36661 gcctttatcg agatatggtt tgagcgaatc aatagcagca tccgtgcctg cacctgcttt
    36721 caccattaac aggatgcgac gaggcgtttc cagagattca acaaactctt tcaccgtata
    36781 gtaaggaacc agtttcttgc ctggattttc ggcaatcact tcttccgtct tttcacggga
    36841 acggttgaaa atagagacgg tataaccacg gctttcgatg ttgagcgcaa ggttgcgccc
    36901 catcactgcc ataccgacta cgccgatctg ttgctttgac attgtttact cctgtcaggg
    36961 ggtggtcacc gccgagtgtg cggcttaaat gtggtataga tgttaactca ctcatagaga
    37021 tggtgaatag cattaccttt gctataccac actgaatatc tcaccttttc cgtcttttaa
    37081 aacttattta tatgaaatta ctgaattact tgttcagtaa ctcaagaata agttttgtct
    37141 tttcttccat cagcggcaca tcaccgcgag attccacatt caaccgcacc accggttcgg
    37201 tgttggaggt gcgcaggtta aagcgccagt cggcaaaggt catgctgatg ccatcggtgc
    37261 gatccaccgc cagcgcctcg cggctaaaat gctgttccac gcggttaatc gcctcaacgg
    37321 gttgcgccag tttgctgttg atctcaccgc ttgccggaaa cgctgccatc cggtcgcgca
    37381 ccagttcgcc cagcgttttt cctttcaggc acaccagttc ggcgaccagc agccacggga
    37441 tcatgccgct gtcgcagtaa gcgaaatcac ggaagtaatg gtgggcgctc atttcaccac
    37501 cgtagatggc gtcttccttg cgcatccgtt ctttaataaa ggcgtgtccg gttttcgaca
    37561 tcaccggcgt gccgcctgcg gcagtcacca catcaacggt gttccaggag agacgcggat
    37621 cgtggatgat cttcgcgccg ggattttttt cgaggaatgc ttctgccagc aggccgacaa
    37681 tgtagtagcc ctcaataaac tgcccttttt cgtcaaacag gaagcagcgg tcaaaatcgc
    37741 catcaaaagc aatgcccata tccgcaccgt gtttgatgac cgcattgcgg gtgtcgtcgc
    37801 ggcattccgg cagtagcggg ttaggaatac cgttggggaa attgccgtcc ggcgtgttgt
    37861 gcactttgat taattccacg ggtgcgccaa gggctttaaa gcgggcttcg atggcgtcca
    37921 ccaccggacc cgctgcgcca ttcccggagt taatcaccag cttgagcggc gtgaggtttt
    37981 tgacattgat ataaccgaac aggtgatcaa cgtaagcgtc acgcagattg atttgctgat
    38041 agcgaccgcg tttggtttca tcaacgggag gaaagtcgtt ggcttctgcc agacgctgga
    38101 catcgcgcag tccggtatca ccgctgatcg ggcgagcccc ttcgcgcacc agtttcatgc
    38161 cgttgtaatc catcgggtta tggctggcgg taacttcaat gccgccatcc acgccgagat
    38221 ggaacgtggc gaaatagatc tcttcggtgc cggacatgcc aatatccagc acatcgacgc
    38281 ccgcatcctg taaacctctc gccagcgcca gttttaaggt ttcgctggtg aggcggacat
    38341 caccgcctaa cacaatggtt ttcggtttga gaaattcgcc ataggcgcga ccaatgcgcc
    38401 aggcgatatc ttcattcagt tcttcgccta atttcccgcg aatatcatag gctttaaagc
    38461 aggttaattt tttcatgtcg ttaccctttt tcaggcaata gttggccctg accgaaacgg
    38521 ccaattttgt ttttgtcgtt atttacatct cggtttcgca ttcattgcct gatgcgacgt
    38581 ttacacccgt ccgtagcgat ccgcgaaacg caccacatca tcctcttcga gataagagcc
    38641 ggagcgcact tcaattaaat cgagcggaat tttccccggg ttttccaggc aatgcatcgc
    38701 ccccagcgga atataaatgg actcgttttc accaagcagt ttgatatcac catcaatggt
    38761 gacttttgcc gttcccgcga caaccaccca gtgttccgcg cggtgatggt gcatctgtac
    38821 cgacaagccc tcgcccggtt tcacggtgat gcgtttcacc tggtagcggt cgcccgcgtc
    38881 gatagagtca tatttgcccc acgggcgata gacttcgcga tgcacccgat gctcatggcg
    38941 accatcggct ttgatctgct cgaccacttt tttcacatcc tgcaccgcgt tacggtcggc
    39001 aatcagcacc gcatctttgg tctgcaccac taccagatct ttcaccccga cggtggttac
    39061 gaggccagat tcggcgtaca catagctgtt ttcagttttg tgattaatca catcgccgtg
    39121 gcaaacgttg ccctcggcgg tgtgggcgct aatttcccat aatgaggacc aggagccgac
    39181 atcgctccag cccgcatcca tcggcaccac aacggcatct gccgtacgtt ccatgaccgc
    39241 gtaatccacc gactcttccg ggcaggcgag aaacgcttct tcatccacac gaataaaatc
    39301 gagatccgga tcgacggcgc tcatcgcttt ttcacaggca tcgagaatat ccggacgata
    39361 ttttttcagt tcttcgagat agcgtccggc gcggaacagg aacatgccgc tgttccagta
    39421 atattcgccg cttgccacat aggcctgggc ggtttccaga ttcggttttt cgacaaactg
    39481 cgccacttca aaggccaccg tatcctgctc acccgcagac acttcaccgc gacgaatata
    39541 gccataaccg gtttctggta gatccggcac aatgccgaag gtcaccagct tgcccgcttc
    39601 ggcatatggc atggcattac gcacggcggc acggaacgcg tcttcatcgg caatcacatg
    39661 atccgccgcc aataccagca ttaacgggtc gctctccggg ctatgacgtt ttgccgccag
    39721 cgccgccagc gcaatggcag gtgccgtgtt tcgccctgcc ggttcgagaa taatgttctc
    39781 ggtaagtttg ttcagttgac gcagctgttc cgcgacaata aagcggtgct gctcattgca
    39841 aatcaccacc gggctttcgc actccacgcc gttcaggcgg cagatggtgg tttgcagcat
    39901 ggtgagatcg cctttcaggc ataaaaactg cttggggtaa agtacgcggg aaagcggcca
    39961 taagcggcta ccggagccac ctgccatcac aactggatag agtttcgact gcgccataat
    40021 tatccccgaa tatcatttat aaattgacgt aacacgttct ctttatcgag tgtgcgttcg
    40081 gcatattcac gtgccaccgt gttgtgtttg ggcagcagga gcgcctgacg gatccccgcc
    40141 accagcgcct cgaccgattc cggttctacg caaaccgcaa tgcccggaaa ggtttcgcaa
    40201 agctgcccca gttctgtgtg ggcctctgcg gtaatcaccg cgttaccgcc taccgccaga
    40261 atattggtca gtttcgacgg caatacggca tctgccgcgc cgcgtttttg caccaccaga
    40321 tggcaatcgc ccatcttcag cagtgcgggt aaagcgtcat acgattgcag cgggaaaaat
    40381 tgcatgttgc gcagtccacg ctgctgcgcc attttttcca gccgcgcttt gccgccgccc
    40441 tgcccgacaa tggcaaaaat cagcggttca tcgcgcagac gatcggcagc ttcaataacg
    40501 ttttccagcc cctgcttttc accaatattg ccggagtaaa gaatgatttt tttgttatcc
    40561 ggcaggtcaa gctggttacg aagggcgtca acatcggcat ctgcaacatg ctgaaaacgg
    40621 gcaatttccg accagttggg gaagaagatg acgttttccg ccgccacgcc tttttcgatg
    40681 gctttattca tcatcgaacg cgaaatcgtg gagacgttat cgacgttatg cagtccgcta
    40741 cgttcgaacg ccgttgccag ctgtgccact ttgctgcctt tgccttttcc ggcaaggccc
    40801 agccccagca tggcgtccac ttcgtaatcc tgaatatgca gcacggtacg cgcaccagaa
    40861 agtttcgcca gcaggcgcat tcccggcgtg caaaacagcg ttggcaccac gccgataatg
    40921 cgatccggct tccagcgacg ttgcgccatc agaggaaaga aactgctgac ggcaaaactg
    40981 ccgagatgta acaggcgttt cagggtgctc ggctgttttg gcacgtacag cgggcagcgc
    41041 cacaccgtgg cggccccctc ttctcgtttg taccgccagg cggaatagtt ctcgcccacc
    41101 tgccactgcg ggtagtaagg cggtgcggta ataacccgca cctcatgacc ttgtgccgcc
    41161 agccattcca ccatctcgcc ggtgtatttg ccgataccgg ttaactccgg cgagtagtta
    41221 atgccgtaga ccagtatttt cataatccgg gtactccggc acgcttctca gcgagaaaat
    41281 aggcgcggct gttagcgtga acatcgttac tggcgagcag cgcgtccggc gtcagccagc
    41341 ggtaatcgtc atgctgctca tccggcagta tcagctcttc ttcagcgact ctgaagcgaa
    41401 aaccgagcac cacatagtga gtggtgaaat ccgtgccgga gaagttatcg tcataaaagt
    41461 gctgccagac accgtaaaac tggcctgctg ttatcggcag acgcagcccc agttccgcca
    41521 tcgtcagccg ctcgaatgcg gcttccagcg tttcgtcttt ctgcacgcgc cctcccggca
    41581 caaaccagta accctgcgcc gggcggttgg ttcttttgcc aagcagaaac tcaccgcgac
    41641 tgttctcgac aataaagtcg agagagacaa gcggggtgga gcgcactacc gtggcaaagt
    41701 cttcctgacg taaaaacatc attacccccg aaagcggtct tgattctcaa ggaaccactg
    41761 gtaagtgctg gcaagccccg cttccagtga gatttcgtga taccagccaa gctgatgcag
    41821 gcgcgtcaca tccagcagtt tgcgcggcgt accatccggt ttgctggcat caaaaaccac
    41881 ccgacctttg taacccacca ctttggcgat ggtttgcgcc agctcgcgga tagtgcagtc
    41941 aacgcccgtg ccgacgttaa tgtgcgacag catcggctgg gtgttctcca gccagacttc
    42001 gtgcgccagc tccatgacat gaatgctcgc cgccgccata tcatcgacgt gcaggaattc
    42061 acgcatcggt gtaccgctgc cccataccac cacgtccggt gcattctgtg ccgtcgcctc
    42121 gtggaagcga cgcagcaatg cagggatcac atgcgaatta ctcgggtgga agttgtcatg
    42181 cgggccatac aggttggtcg gcatgactga gcggtaatcg cgtccgtact ggcggttgta
    42241 tgattcgcac agtttgatcc cggcgatttt ggcaatggcg taaggctcgt tggtcggctc
    42301 cagcgtgccc tgcaataact cgctttctgc catcggctgt tttgccagtt tcgggtagat
    42361 acaggacgac ccaagaaaca gcagtttgtt cacgtcgttc tgatgcgcgg cgtgaatgat
    42421 gttgctctca atcatcatgt tctggtagat gaaatccgcc ggataggtgt tgttggcaac
    42481 aatgccgccc actttcgccg ccgccagata gacctggtca atgcgttcgc tggcaaagaa
    42541 atcatgcacc gcgcggctgt ccagcaggtt cagctcgtcg cgggtgcgta ataccagttc
    42601 cacatcaccg cgctgttcga gctgccgcct gatggcagaa ccgaccatcc cacgatggcc
    42661 agcgataaaa atgcgttgtt tactcatgct tatgactcca gcgcgatcgc cacgtcgtag
    42721 ccgtgagatt tcagcagaga gtgttttttc gccgcttcga ggtcattagc caccatttca
    42781 gacaccatct ctctgagggt gatttccggt ttccagccca gtttttcgtg cgctttggtc
    42841 gggtcgccga gcagcgtttc aacttcagcc ggacggaagt aacgcggatc aacggcgata
    42901 atcacatcac ccggtttaac gcccggcgcg tcatgcccgg tgacggaaac cacaatgccc
    42961 ttctcttcaa cacccgtgcc ttcaaagcgc agtttgatgc ccagctgtgc cgctgccatt
    43021 tccacgaact gacgcacgga gtactgaacg ccggtcgcga taacgaaatc ttccggctgt
    43081 tcctgttgca gcatcatcca ctgcattttt acgtagtctt tggcgtggcc ccagtcacgc
    43141 agggaatcca tattgccgag gtacaggcac gactccagcc cctgggcgat gttggcgatt
    43201 gcgcgggtga ttttgcgggt aacgaaggtt tcgccgcggc gcggggattc atggttgaag
    43261 agaattccgt tacaagcgta catgccgtag gattcgcggt agttaacggt gatccagtag
    43321 gcgtacagtt tggcgaccgc atacggagat cgcgggtaga acggcgtggt ttctttctgc
    43381 ggaatttcct gcaccagacc atacagttca gaggtggaag cctgatagaa acgagttttc
    43441 ttttccagac cgaggaagcg gatcgcctcc agcaggcgca gcgtacccat cgcgtcgacg
    43501 tcagcggtat attctggtga ctcaaaagag accgcaacgt ggctcattgc gcccaggttg
    43561 tacacttcat ccggctgtac ttcacgcaaa atgcgcgtca ggttagaggt atcactcagg
    43621 tcgccataat gcagatggaa tttcgggttg caggtgtgcg gatcctgata aatgtgatcc
    43681 acgcgctcgg tgttgaatga cgatgcgcga cgcttaatac catgcacctc gtaacctttt
    43741 tccagcagaa actctgccag gtaagaaccg tcttgtccgg ttacaccggt gatgagagcg
    43801 acttttgaca tgtcttattc ctctgtattt ttgaatttat tcagtttcaa cgcgttcgcg
    43861 tatcaccact gcgggattcc cccggcaaac cacatttgcc ggaagcgatt taaaaacact
    43921 gcttcgtgca cccacgacgg tgccgtcgcc gattgtgacg cctggggcaa caaagacatc
    43981 ggttgccagc cagcatttct cgccaatcac aataggcgtg gcgttaatgg tgaaatgttg
    44041 acttgcatgg tcgtggctac cggtgcataa ataacttttt tgcgatatca ccgaatgtgc
    44101 gccaatggtt atttcaccga gggtatataa attgacgtca tcgccgaccc acgcgtaatc
    44161 acctaaggtt aatttccacg gataggtaat ttttactgac ggacgaataa ctacgttttt
    44221 tcctattttt gctccgaata aacgtaataa aaaagcccgc cagcgataca atacttgtgg
    44281 cgaccaggca aatattgttg cctgtactgc ccaccataat tgcactttaa tagcgttgcc
    44341 gccccggaac cctttcggca ccgagaaacc gcttaaatct tgcattggtt ttccttatat
    44401 tcagactttg ttatataagg ctttcgtctt tgaggtagta cgttggcgta aatgccagga
    44461 taattcagcc caaaagccag gcacatgtaa tatttgtcgt tggacttttt tcgcgtcagc
    44521 acacaattcc atattattgg tggtagatac cccacccatg gaaaattcag acaccaggcc
    44581 attgagtttt ttaaatgcat aaccggcttt atacattttg gctgccagcg cgtagtcgga
    44641 agaaacttta tattccaggt cataacgcca ttttttcaag ccggatacag ggaaaaatat
    44701 cgcctgatga ctggcgggca ggctgtgata aatataccag cccggtttgg cgctacgttt
    44761 aattttatgc ccgtcgccaa aatccagcag cgcatcgccg gtgatcatca tgttatcttt
    44821 ttgcattttt aacttacgga caaaatttgc ggcatcctga tgaaaaatat cgcccgaatt
    44881 gagaaacaac gcgaacttgc cttgtgccat cgtaatgcct ttgttcatgg cgtcgtagat
    44941 accgttatct ggctcgctga caaagcgtag gttatagata ccattgagat tttccagata
    45001 ctcacgggtg ccgtcattgg aaccgccatc gacaacaatc cattcgaagc tgatatcttc
    45061 cgcctgcgcc agatgcgcca gcgaggcatg tgttttgact atcccttcga ggttacgaaa
    45121 cgcgacagtg attatgctaa gcaacatgtt cttattctta cctcgtaata tttaacgctt
    45181 ttcgcaaaat aaatggacag acgattaaaa atgcatattc cgggctaaat atcgaaccgg
    45241 taaaaaacag cgataccgga gtaaaaagat ataattgcac gcgaaaattg cggttatcac
    45301 caaaagcgtt gatcatcatt tttatcactt tccccatgta ccacagcgat aaaaacactg
    45361 cgaaccagga aaaataaata atcagcagat acaaaccatt gtctatggtt tttccgacat
    45421 ccgcaccgtt aaatattccg aatgatgcga cgtattcata aagtgagcca aatctgacta
    45481 caccgtcaat atgggtcaaa gaataaccga ccatcaccag cggaccgaca atacgataat
    45541 acgatgacga tccttccgta cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga
    45601 ttaccacacc aaccaggaat acagccagag aaatcaatgc caacggtaac tttttcttaa
    45661 tcgcctcttt attcagatat tgaaatgccc actccagcaa ataaaacagg ataaaagtca
    45721 taacccctga aaacgatccg gataatatta tccctgcgag aatcatagca tcggttttag
    45781 gcgttttgat accaaactgt ttgatgctga gccaaattga gattaatgcc agagcgaaaa
    45841 atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc gaatttaatg aaattcagta
    45901 catagctgtt gctgtaaatc agatatttcg aaatcgactc cataatactg ctgccaccgg
    45961 tgaggataat ttgcgccatc tccaccgccg ccagcgccac caccagcccg atcaccagat
    46021 aaaagaaacg taatatcttg cgatggttgt gtggcgaaat cgttttaaag cgaatactcc
    46081 acaccatgcc aataatgatc acaatataga caaacagcat ggttgaagtg acgtatttac
    46141 tggcatccag cgactgacca aacagatagt taaacgccgt gagtcccgca ccaatcccta
    46201 aggcaatcat caatttttta acgctgatgc gttctaaaaa cagcagtaac aagacaggta
    46261 aaaaagtgac gatggtgatg gggaaacttt cgccaagctg ggcgattttg acgttaacca
    46321 gcaggtagat cagcggcagc agcaggtagc tacagattct gatagaagtt gacatactcc
    46381 tccagcatct gttgtccact gtaggtggcg cggctgcgtt ggctgaactc agccagcgtg
    46441 gtaccaaata tcgcctgcgc gatttccggt ttgcttaact gcaccagttg cagcacgtct
    46501 tcttcgtcga cggttttacc gccggatttt tgcaacactt cccgtgccgc gtcgctatgg
    46561 gtggcaatca ccggcacgcc aattgataac gcctcacaca aaatcagcgg gtagttatcg
    46621 acgcgagaac tgaataccag cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg
    46681 tcagtttcaa agccgtgatt aaccacgttg ccagcggtga acggcgagaa cttaccaaag
    46741 gtatgcagtt cgattttgtc gcccagcgcc atcatctcac gcaccagttg ctggttagtc
    46801 ttgccgtcgt aacgcaagtc atgcgccacc accgcgattt tcggcttgcc ctgggtttcg
    46861 cgcaccggag gcaagtccgc cagaatcgct tcggttgcca tatcaatacc attattgata
    46921 atccggcaac gccctggacc gtacaggcta ttgaaagcgt cagccacatg ctggctgggg
    46981 gaaataaact gacagcccag cgccagcatc tcacggaata actggcgttt gcccgccacc
    47041 agctggtgtg cgcgatcaat cttcaccggc ggatagttat ttaaggtcgg gcatttctgg
    47101 cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc agcgtccggt aacactccag
    47161 tggtcgtgca gcgtccagac cagagtgacg tccggtttat ggtttttcac tttttcgcaa
    47221 aagcgcacca cgctcttaag atttagccag tagctgtgca gcacatgaaa atgcaggacc
    47281 accggacccg gtgtgcgagt aatggtgcga tataactcat tgaaattgcc aaacagatcg
    47341 cgattaaaca gacgaaacag ggcaatattc gccatcgcgg tcatccgcgg cgtatgtttg
    47401 atgacctgcg gatagttctg atggctgacg ctctctttgc cgcctttgcc gtaaccgtag
    47461 acaaaatgtg acgccagccc ctgttgcagc gcacgctggt ggagatctaa cgccacacct
    47521 gctgccccgc cttccgccag tcgcacatta aattgcagaa tattcattta attaccttca
    47581 ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac agagtcgagc accacgctgc
    47641 ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc aagaataatg acgttggcac
    47701 cgagttcgac gccgttgcca atgtgtggac atgccatgtt atcggcacca cgattgccga
    47761 tagtgacgcc gtggcgaatg gtgaaatcat cccccgctac cacgttttta ttgatcacta
    47821 cggcgtaacc gtgatggata gtaaagcggc ggccaatggt cgcggcggcc tggatttcat
    47881 aaccgaaaaa gcattcggtg ataatgcgat acagcaccag cagcggggcc gcccacagat
    47941 tgttgaggac gttctttttg cgccacaccg aacaaaaatg agcaacacga taggcaagaa
    48001 ccatgcagca cgggcgtaaa ctccagctgt tggcacgcag atcttccagc atctttagcg
    48061 cccccgaata ccatcagcca gacgtttgcc gttacgcacc gacagcagcg tcagcaaagt
    48121 gcgccaggtc atacgtttat tgcgaatctg gtagagggta aacagctgat attttttgct
    48181 tgcacggtca aatttatctt tgtgctttcg gtaaaagtgg aagtacccgg agaatttttt
    48241 cggtgacgag gtgatctgca tttcaccgtg attgatatgc aggatctgcg tcgcttcttc
    48301 cactttccac ggttcgccgt actccaccac catccgcagg aagatgtcgt aatcctgtgc
    48361 tgctttcagt tcggtatcga acaaacactc tttgaaacgc catgcccaag taaagacctg
    48421 attgccaatg atattgcgtt tatagaacag gcggcgtgag tacggcgatt tgggatacag
    48481 tggtaagctt gccggttggg aataaacttc tccctggcat acgtagtcgt tagcgtacaa
    48541 aaaggcgtga gtgaccagtt gctgtttatg ggcgaggaag acgctcagac ggttgggtgt
    48601 ccattcgtca tcgtcatcga tcccggtaat gtattcccct tgcgccagca taatcgcctg
    48661 gttacgtacc gcgcacgccc cgctgttaat gtcgttgtga atgtaggtga tacgcggatc
    48721 gttgagggca gtgacgtact gttgcagttg ttcccaggag gtggagcaat catccacgat
    48781 gatcatctcc cagttgctgt agtcctggcg caggaccgat tttatcgccc gaatcgccag
    48841 ttgttggcgg ttccaggtcg gcatatagat tgaaatcagc ggattctctt tcataggttg
    48901 ttctcccggc ttatgagctg gtttgcttgc cggatgcggc gtgagcgcct tatccggcct
    48961 acggggcggt gcgaatgcag gcgtcgttat ttcgcatccg acttatattc gtattcgtaa
    49021 tagccataat cctgatacgc gctggcgcgg cggaagatgg agttcagaat cacccctttc
    49081 accggaatac cgttttgctc aaagcggctc agactggttt ccacttcttt caatgtgttg
    49141 accgcatagc gcgccaccat taacgtggtt ccagcgtgac gcccgacaat tgctgcatcg
    49201 gtcactgcca gaatcggcgg cgtatcaatc aacaccaggt cgtagttttt actcgcccag
    49261 ctcaccaatt cggcaaagcg ttcgctcatc aacagttcag aaggatttgg cggcacctga
    49321 ccgcgcggga tcaggtcaaa tttggcaata gaggtcggtt tagctgcggt agtaatatcg
    49381 ccctggccaa tcagaatttc cgacaggcca ttgacgttat tggtacccaa cagctcgtgg
    49441 gtgtagcctt tgcgcatatc gcagtcgatc aacaacaccc gtttattggt ctgactgatc
    49501 accgccgcca ggttggcgca gacaaaggtt ttaccgattg acgggctcac ccctgtcatc
    49561 atcaacacat tgttctgcgc ctgcatcatc gcgaagtgca gactggtacg cagactgcgg
    49621 atggcttcaa tcgccagatc ggttggattc cccaccgcca gtagctggct ctgtttatag
    49681 cgtttaatcc ctttgatggt tttgacgcta tcgcgcgctt tctgccattc cgacagcggg
    49741 atgctggcat agacgctgat accatgttct tccagcacct gcgggctttc gatgccgcga
    49801 ttaaacaacg agcgcagcag cacacccacg atagagagca tcaggccgag gataattgcg
    49861 ccgaggataa tcagtccttt cttcggtttc agcacgccag gctgggtgat tgccgggtca
    49921 acaatgcgca catcgccgac ggtgctggct tcggtgattt tcagctcctg ctctttattc
    49981 agcagttgca tatagacctg ctgaccagac tcgacatcgc gggtcaggcg cacaatctcc
    50041 tgctgggttt tcggcatcgc cgttacgcga ccgttaagtt tggctttctc ttcttccagc
    50101 gcctgacgtt tctccagcag cgtgcggtac gccgggtgaa ctttggtgta cagcttggag
    50161 atttccgcct ctttaaaggt cagttcgttc aactgcgcgt cgatgttcac catcgaatcg
    50221 agcaccgctt tcgcttccag cggcagatca acagaatctt tatcctgacg gaaggcattc
    50281 agtttgtttt cggcaacatc gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa
    50341 gcgaggcttt tcgacgcttc cgccgatttg cgctcaatat tttgttcctg atagttacgg
    50401 gcgatgctgt taagaatgtc gcggatctgt tcgcgatctt caccggtata agtcaggctc
    50461 agtacgcctg cgtctttgcc gttctccgtt accgtcaggc tgttttgcag ttggttgatc
    50521 atccccaacg tggagtattt ggtgacggta aactcactgc ccgggcgggc gtgaatggct
    50581 tcaaccatca gcgtgacgcc ttcttttttc agcatctggc ccgcttgccc acgggcgcta
    50641 aagccgccat cactgctcag ggtgtagttt ttattgtcca gcacattaag cgtaaacacc
    50701 tggcccgcca tctctttcgg gcggttaaag gtagtcactt tcaccgtctc gttctggcgt
    50761 cccatcagtc gctcccagcc cgccccgaaa atcgggaacg tgtttttgct cactgcaata
    50821 tcgaggtcga gatcgtccac cgttttacca agcaccagac gcgaacgaat caactggatc
    50881 tcggcgtccg atgcaggcgg tttgttggct aatgccgaac caatgtcctg cactaacgaa
    50941 ttgccgctgt tttgctcgat ttgtaccagt gcgtcggcac tataaatcgg cgttgcgaag
    51001 aaggtgtaaa ccacggcaca gagggcaaat acagcggtga tgccaatcac ccaccagcgc
    51061 gcttcaatga cggtgccgac caggcgacca atatcgattt catcactgcc cgttaccgga
    51121 gcggcatgtt gttttacttt ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg
    51181 cccactggcg ggcagaccgt tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt
    51241 tgcgatacgg atcggggatt tcacattcgt tatcccagtg accaaacagc atcactttgc
    51301 cgcgcatctc aggtgccatc tcgcataagc gttcgatatg gcgcttttcc atggtcaaaa
    51361 tcaggtcata gttgcgacac aggcggcggc tgatttgtcg tgcacagtgg ccttccagag
    51421 acagttgatg ttctgcggcg acgctgatag cggtaggatc agcgccctta ccgaccagcg
    51481 cgccgagtcc agcggactcc actttcagtt ccgggtgata acgttgcagt aagcgttccg
    51541 ccgtcgggga acggcaaata ttgccgacac agacaactaa tatgttgtta aacatgacga
    51601 ttaccagtta tgaatgtcgc tggctgtatc cgtcatgtaa cggacaccgc taatagttgg
    51661 caacaactga ttgatcagac ggttccagcg ggaaaccggg gcggtggtga catacaccac
    51721 gtcataaggt tgcaggcgga attctgtcgc catcaccagc gacgtggcat cggacatatc
    51781 cagctggtag atattggcaa tcttgccatt acgcccgccc tcgcctttca gcggacgaat
    51841 gacaaagatg ccgctggcgt tggaggtggt catgtcgata ccttcagcat tacccagggc
    51901 ttcagtcagg gtcatgccgc taaagtccat tttcagggtg ctctgtttct tcacttcacc
    51961 catcacaaat actttcagat catcattacg cggcacgtag agaatatcgc cgggataaag
    52021 cagtcggttc tgattgaggt cgccgttttg catcagcgcc tgcaaagaaa tgcgttcttc
    52081 acgaccattg tgtgttagca ctacgttgcg ccagtcagcg gtgtcggtca ggccacctgc
    52141 ggcgttgatg gcgtcgagaa tagtcagtgg cacgttggtg atcgcctgtt gaccggattt
    52201 attcacctga ccggagatat aggccttttg tgagcggaag gcggcgatat taacgtccac
    52261 ctgcgggtca gcgatgtacg tcgctaagcg cccggtaata tcactgcgga tttcagcgag
    52321 cgttttcccg actacgtgaa ccttgccgat atacgggtaa aacatagtgc cgtcaggctg
    52381 tacccagttg ccggtgtcgc tggagctgcg gtactgaccg gctggcgtgg tgagttccgg
    52441 gtgatcccag acggtgacat tcagaacgtc gcccggcccg acgcgatact ggtaattcgc
    52501 gatctcgctt tccagcgtca tattggggcg cgctacattc gggcgtgggc gtaattggtc
    52561 aatcaggcgc ggggtcagcg gataaacatt caccattttg tcgagatcga aatcagcgtc
    52621 ctgctgtttg atgacgtctt tgcccatcgt cgacatattg ctgcccggaa gtactgtgca
    52681 accgcttatc aaggttactg acaccaataa tggcatcaat ttcattttgg atttcatcat
    52741 tgtttattta tcactttggc agagtaatta tcctgtgcac tattaatagc aatatcgcca
    52801 tgcacattta ccttgcagtt aattgaataa aaatttaact ggcatcagtc ctaaaaaaat
    52861 tgatttcatc cgcaggctat tgacagaata attcagactg gtctttcagg catccagaca
    52921 cgctaccgcc cctggctttt tagccaccaa tacactgatt tagcttaatt tttcaaaccc
    52981 tctctgcata caatcgttga tgagaaaggg ttattgcgga aattaacttc cgaatataag
    53041 gtgacattat ggtaattgaa tattggctct ccaataatgc aggaggaagt gttacagcta
    53101 acggaatagc aggcaaggta acaattcggc aattggctat ttttaagaat tatattaagt
    53161 gtcattcaat atggttttta ggagtttctt taggttgaca atattta
//
LOCUS       DAZOZY010000006        49499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZOZY010000006 DAZOZY010000000
VERSION     DAZOZY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.006"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            49..681
                     /gene="comR"
                     /locus_tag="ACHURQ_000307"
     CDS             49..681
                     /gene="comR"
                     /locus_tag="ACHURQ_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR0822187.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(743..1282)
                     /locus_tag="ACHURQ_000308"
     CDS             complement(743..1282)
                     /locus_tag="ACHURQ_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0822188.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1509..2813)
                     /gene="ndh"
                     /locus_tag="ACHURQ_000309"
     CDS             complement(1509..2813)
                     /gene="ndh"
                     /locus_tag="ACHURQ_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR0822189.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3221..3763)
                     /gene="ycfP"
                     /locus_tag="ACHURQ_000310"
     CDS             complement(3221..3763)
                     /gene="ycfP"
                     /locus_tag="ACHURQ_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR0822190.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3786..4811)
                     /gene="nagZ"
                     /locus_tag="ACHURQ_000311"
     CDS             complement(3786..4811)
                     /gene="nagZ"
                     /locus_tag="ACHURQ_000311"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR0822191.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4822..5646)
                     /gene="thiK"
                     /locus_tag="ACHURQ_000312"
     CDS             complement(4822..5646)
                     /gene="thiK"
                     /locus_tag="ACHURQ_000312"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR0822192.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5627..6268)
                     /gene="lpoB"
                     /locus_tag="ACHURQ_000313"
     CDS             complement(5627..6268)
                     /gene="lpoB"
                     /locus_tag="ACHURQ_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR0822193.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6282..6659)
                     /locus_tag="ACHURQ_000314"
     CDS             complement(6282..6659)
                     /locus_tag="ACHURQ_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR0822194.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6662..7021)
                     /gene="hinT"
                     /locus_tag="ACHURQ_000315"
     CDS             complement(6662..7021)
                     /gene="hinT"
                     /locus_tag="ACHURQ_000315"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR0822195.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7355..9544
                     /gene="fhuE"
                     /locus_tag="ACHURQ_000316"
     CDS             7355..9544
                     /gene="fhuE"
                     /locus_tag="ACHURQ_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR0822196.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(9604..11037)
                     /gene="ptsG"
                     /locus_tag="ACHURQ_000317"
     CDS             complement(9604..11037)
                     /gene="ptsG"
                     /locus_tag="ACHURQ_000317"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR0822197.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11332..12129)
                     /locus_tag="ACHURQ_000318"
     CDS             complement(11332..12129)
                     /locus_tag="ACHURQ_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0822198.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12140..13144)
                     /gene="holB"
                     /locus_tag="ACHURQ_000319"
     CDS             complement(12140..13144)
                     /gene="holB"
                     /locus_tag="ACHURQ_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR0822199.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13141..13782)
                     /gene="tmk"
                     /locus_tag="ACHURQ_000320"
     CDS             complement(13141..13782)
                     /gene="tmk"
                     /locus_tag="ACHURQ_000320"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0822200.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13772..14794)
                     /gene="yceG"
                     /locus_tag="ACHURQ_000321"
     CDS             complement(13772..14794)
                     /gene="yceG"
                     /locus_tag="ACHURQ_000321"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR0822201.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14797..15606)
                     /gene="pabC"
                     /locus_tag="ACHURQ_000322"
     CDS             complement(14797..15606)
                     /gene="pabC"
                     /locus_tag="ACHURQ_000322"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR0822202.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15726..16967)
                     /gene="fabF"
                     /locus_tag="ACHURQ_000323"
     CDS             complement(15726..16967)
                     /gene="fabF"
                     /locus_tag="ACHURQ_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR0822203.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17055..17291)
                     /gene="acpP"
                     /locus_tag="ACHURQ_000324"
     CDS             complement(17055..17291)
                     /gene="acpP"
                     /locus_tag="ACHURQ_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR0822204.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17502..18236)
                     /gene="fabG"
                     /locus_tag="ACHURQ_000325"
     CDS             complement(17502..18236)
                     /gene="fabG"
                     /locus_tag="ACHURQ_000325"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR0822205.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18249..19178)
                     /gene="fabD"
                     /locus_tag="ACHURQ_000326"
     CDS             complement(18249..19178)
                     /gene="fabD"
                     /locus_tag="ACHURQ_000326"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR0822206.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19194..20147)
                     /gene="fabH"
                     /locus_tag="ACHURQ_000327"
     CDS             complement(19194..20147)
                     /gene="fabH"
                     /locus_tag="ACHURQ_000327"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR0822207.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20215..21285)
                     /gene="plsX"
                     /locus_tag="ACHURQ_000328"
     CDS             complement(20215..21285)
                     /gene="plsX"
                     /locus_tag="ACHURQ_000328"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR0822208.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21366..21539)
                     /gene="rpmF"
                     /locus_tag="ACHURQ_000329"
     CDS             complement(21366..21539)
                     /gene="rpmF"
                     /locus_tag="ACHURQ_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR0822209.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21591..22112)
                     /gene="yceD"
                     /locus_tag="ACHURQ_000330"
     CDS             complement(21591..22112)
                     /gene="yceD"
                     /locus_tag="ACHURQ_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR0822210.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22311..22895
                     /gene="yceF"
                     /locus_tag="ACHURQ_000331"
     CDS             22311..22895
                     /gene="yceF"
                     /locus_tag="ACHURQ_000331"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR0822211.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23007..23966)
                     /gene="rluC"
                     /locus_tag="ACHURQ_000332"
     CDS             complement(23007..23966)
                     /gene="rluC"
                     /locus_tag="ACHURQ_000332"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR0822212.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24539..27724
                     /gene="rne"
                     /locus_tag="ACHURQ_000333"
     CDS             24539..27724
                     /gene="rne"
                     /locus_tag="ACHURQ_000333"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR0822213.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27919..28872)
                     /gene="flgL"
                     /locus_tag="ACHURQ_000334"
     CDS             complement(27919..28872)
                     /gene="flgL"
                     /locus_tag="ACHURQ_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR0822214.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28884..30527)
                     /gene="flgK"
                     /locus_tag="ACHURQ_000335"
     CDS             complement(28884..30527)
                     /gene="flgK"
                     /locus_tag="ACHURQ_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR0822215.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30593..31534)
                     /gene="flgJ"
                     /locus_tag="ACHURQ_000336"
     CDS             complement(30593..31534)
                     /gene="flgJ"
                     /locus_tag="ACHURQ_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR0822216.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31534..32631)
                     /gene="flgI"
                     /locus_tag="ACHURQ_000337"
     CDS             complement(31534..32631)
                     /gene="flgI"
                     /locus_tag="ACHURQ_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR0822217.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32643..33341)
                     /gene="flgH"
                     /locus_tag="ACHURQ_000338"
     CDS             complement(32643..33341)
                     /gene="flgH"
                     /locus_tag="ACHURQ_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR0822218.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33394..34166)
                     /gene="flgG"
                     /locus_tag="ACHURQ_000339"
                     /pseudo
     CDS             complement(33394..34166)
                     /gene="flgG"
                     /locus_tag="ACHURQ_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(34338..35093)
                     /gene="flgF"
                     /locus_tag="ACHURQ_000340"
     CDS             complement(34338..35093)
                     /gene="flgF"
                     /locus_tag="ACHURQ_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR0822219.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35113..36318)
                     /gene="flgE"
                     /locus_tag="ACHURQ_000341"
     CDS             complement(35113..36318)
                     /gene="flgE"
                     /locus_tag="ACHURQ_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR0822220.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36343..37038)
                     /gene="flgD"
                     /locus_tag="ACHURQ_000342"
     CDS             complement(36343..37038)
                     /gene="flgD"
                     /locus_tag="ACHURQ_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR0822221.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37050..37454)
                     /gene="flgC"
                     /locus_tag="ACHURQ_000343"
     CDS             complement(37050..37454)
                     /gene="flgC"
                     /locus_tag="ACHURQ_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR0822222.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(37458..37874)
                     /gene="flgB"
                     /locus_tag="ACHURQ_000344"
     CDS             complement(37458..37874)
                     /gene="flgB"
                     /locus_tag="ACHURQ_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR0822223.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38029..38688
                     /gene="flgA"
                     /locus_tag="ACHURQ_000345"
     CDS             38029..38688
                     /gene="flgA"
                     /locus_tag="ACHURQ_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR0822224.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            38764..39057
                     /gene="flgM"
                     /locus_tag="ACHURQ_000346"
     CDS             38764..39057
                     /gene="flgM"
                     /locus_tag="ACHURQ_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR0822225.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39062..39478
                     /gene="flgN"
                     /locus_tag="ACHURQ_000347"
     CDS             39062..39478
                     /gene="flgN"
                     /locus_tag="ACHURQ_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR0822226.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39518..41053)
                     /gene="murJ"
                     /locus_tag="ACHURQ_000348"
     CDS             complement(39518..41053)
                     /gene="murJ"
                     /locus_tag="ACHURQ_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR0822227.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSLAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41163..42086)
                     /locus_tag="ACHURQ_000349"
     CDS             complement(41163..42086)
                     /locus_tag="ACHURQ_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0822228.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42088..42735)
                     /locus_tag="ACHURQ_000350"
     CDS             complement(42088..42735)
                     /locus_tag="ACHURQ_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR0822229.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42746..43330)
                     /gene="rimJ"
                     /locus_tag="ACHURQ_000351"
     CDS             complement(42746..43330)
                     /gene="rimJ"
                     /locus_tag="ACHURQ_000351"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0822230.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43566..44774
                     /gene="mdtH"
                     /locus_tag="ACHURQ_000352"
     CDS             43566..44774
                     /gene="mdtH"
                     /locus_tag="ACHURQ_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR0822231.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            44838..45485
                     /gene="grxB"
                     /locus_tag="ACHURQ_000353"
     CDS             44838..45485
                     /gene="grxB"
                     /locus_tag="ACHURQ_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR0822232.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45619..46179
                     /locus_tag="ACHURQ_000354"
     CDS             45619..46179
                     /locus_tag="ACHURQ_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0822233.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46285..47331
                     /gene="pyrC"
                     /locus_tag="ACHURQ_000355"
     CDS             46285..47331
                     /gene="pyrC"
                     /locus_tag="ACHURQ_000355"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR0822234.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47405..47650
                     /gene="dinI"
                     /locus_tag="ACHURQ_000356"
     CDS             47405..47650
                     /gene="dinI"
                     /locus_tag="ACHURQ_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR0822235.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            47940..48194
                     /gene="bssS"
                     /locus_tag="ACHURQ_000357"
     CDS             47940..48194
                     /gene="bssS"
                     /locus_tag="ACHURQ_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR0822236.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            48309..49427
                     /gene="solA"
                     /locus_tag="ACHURQ_000358"
     CDS             48309..49427
                     /gene="solA"
                     /locus_tag="ACHURQ_000358"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR0822237.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        12025 a        13336 c        12281 g        11857 t
ORIGIN      
        1 tatttttata ataacctttc attcacagtc accaggttca acggcatcat ggcaactgac
       61 tcaacacaat gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg
      121 cttgataagg ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac
      181 ctcgtcgaag cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag
      241 gggttatttc gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca
      301 cagctgtttt gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc
      361 gccaactgct ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc
      421 accctctccc cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa
      481 gagcgcactt tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac
      541 tgtgacgtga cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc
      601 gcacgcgaag gtgcatcgct ggaaaaactg atgcagattg ccggaacgac tttgcgttta
      661 tggcccgaac tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc
      721 caaattgcag agtacagcct tgttatactt tgttattcaa aatcagttgc ccgttactat
      781 ctagcgggat ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa
      841 tcttataggt cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac
      901 gctgttgcgt agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg
      961 catatccacc acccagcgcc cccacaacag tggcgacatc tttaccacga ccaccaccaa
     1021 actgatgccc tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt
     1081 catcctgcac cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt
     1141 taaccgtttc cttgattggg gttgcagaaa caacctgagc gtattgcggg ccccgttcaa
     1201 acacgttcag actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac
     1261 ccgccaacat tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca
     1321 agcgaaggag aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata
     1381 cttaaggatt cggagaagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag
     1441 gcctaccgct cgtaccattt gtccagggcg gatgaggcgt ttatgccaca tccgccagtg
     1501 tacgtcgatt aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc
     1561 atcattaatc cggttttaaa gtaaccatgc agcgcaatct gatgcattcg gtatagcgag
     1621 atatatacaa agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc
     1681 atcaggctac cgacagtgga gaagttcgac agcgatacca gcgaaccatg atctttatac
     1741 tgataatttt tcagcggttt accgttcatc tgcgccagaa tattgttcat tgcgcaggtc
     1801 gccatctggt gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat
     1861 gacgcgcagt cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag cgtcggttcc
     1921 accaccagct ggttgatacg gttggtttca agaccaccga tatctttcag gaagtctggc
     1981 gctttgatcc cggctgccca caccatcaga tcagcctcaa tatattcacc atctttagtg
     2041 tgcaggccgc cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt
     2101 ttcgttagct cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg caaaatacgt
     2161 tctcccgctt ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag
     2221 ctgtgcagtt gcttgaccgc attgtgcaat tcagcggaga gttctacacc cgtcgcgccg
     2281 ccgccgacaa tcgcaatgtt cactttacca ttcgcgccca ggttggcgga gtatttcagg
     2341 aacagattca gcatctcctg gtggaagcga cgcgcctggt gcgggttatc gaggaaaatg
     2401 cagttctctt tgacacctgg cgtattgaaa tcgttagagg tgctacccag cgccattacc
     2461 agggtgtcat aggcgatttt acgttccgga accagcagtt cacctttctc atcgcgcagt
     2521 tctgcaatag tgattgtttt cgcttcacga tcaatatcaa tgacggaacc cagctggaac
     2581 tggaaaccat gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc
     2641 gagccagtcg ccacttcgtg cagcagcggt ttccacagat ggctgtggtt acgatcgacc
     2701 agcgtaattt tggctttttt cttgcgtccc agcttatgcc ccagctgtgt tgccatttcc
     2761 agcccaccag caccgccgcc gacaatcaca atttttttca atggcgtagt caacgtgacc
     2821 cccttaaatt tattaaccaa ttgttaatta atggttataa acatagctct taattaacaa
     2881 caggttacag caatgaaaat gttcttcgct attgagaata acatgaatgg tgcattggtc
     2941 ataccaaaat tgatgttaat caagttttgt tgctgaaaag ataggcgact ttaccgggtg
     3001 agatcaaaaa acagagttta ttgtttatta tttgagcaag tccggaaggg tgatataggg
     3061 tgacgcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca
     3121 ttgatgctcg actgtcggat gcggcgtaaa cgccttatcc gacttacaga attcggagcg
     3181 catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg
     3241 ccttaatgcg ctgcaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc
     3301 agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta agcgcttcat
     3361 cattacgcga caaaatcacc aggcaacgat cgcgattctt ctcgcggaag ttggtcacac
     3421 acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat
     3481 aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt
     3541 ccgcccagta tccgcccaga ccaacgccgc aaatcagcgg acgctcgtcg acgttcagtt
     3601 gcaacatttt gtccacttct ttgagcagat gctgcatatc atgtttcgga tgccgcgtac
     3661 tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt
     3721 tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc
     3781 cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg
     3841 ctgatcgcct tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga
     3901 tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc
     3961 cctttacgat tattgcagac caggatcata tcgcaacctg cgtccagtga tgcctgcccg
     4021 cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatt
     4081 acaccgtcaa aacacagttc ctgacgcaaa acggttttca gccagtagga agaaccgctc
     4141 gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta
     4201 ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga
     4261 cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag
     4321 tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt
     4381 gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc
     4441 ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc
     4501 atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca
     4561 aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct
     4621 tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt
     4681 tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc
     4741 ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt
     4801 actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc
     4861 cagcctgata aattgttgat cgccgatttg tcgccagcgg tactcaaacc accctgcttt
     4921 gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat
     4981 cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca
     5041 caccgccgcc agttccagcg cgatatcacc atctccggca tactcccagt cgatgagttt
     5101 taaccctgac gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa
     5161 gcgtaaaggc cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac
     5221 agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca acagcgttat
     5281 tcgccagcca aaacgtggtt gttgatgaag ataatacagc aagcctgcca gttcgttggt
     5341 atccggcaaa tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag
     5401 atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg
     5461 caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc
     5521 ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg
     5581 aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa
     5641 cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta
     5701 gggtcggagc gttaacgttg ccagaggcgc tggagtacag cacgtaatga gcgccgacat
     5761 tgcgggcaat gcctatggct ttactacggg tacctaaact gtcctgcggc gacaaaccta
     5821 actgttgctt agccatcgac agctgctggg cggaaaccag ggtaaatttc ccgttattag
     5881 ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt
     5941 tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct
     6001 tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg
     6061 gtgccgtttg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg
     6121 gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt
     6181 ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa
     6241 tcaaggcgta gcgactcatt tttgtcatca agattcaccc cttacaaata tagataaagt
     6301 ctgactttgt gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg
     6361 ggaatgctaa cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac
     6421 cagtaaaaac gataatgaac ggtaacgggt tcttgccttt cgttataaag cgttgaggat
     6481 gctgaaggtt gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc
     6541 aataaagatg actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa
     6601 cgacagccca ccagcagtaa caacgccaga gacaccagcc caaagcatcc ttttctcatc
     6661 gttacagacc tttatgcgcc agcattggtc ccagcggacg gccacccaac aagtgcatat
     6721 ggatgtggta aacctcttgt ccgccatggc ggttggtatt catgatcaga cgatagccat
     6781 cttcggcaat accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg
     6841 cctgctcatg ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca
     6901 gaatatgcgt tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt
     6961 agacgatatc ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca
     7021 cgacattttc ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg
     7081 agtatagagt atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc
     7141 aaaaaatcgt tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc
     7201 tgaaaagaca ttctctgttt ggtttacacg acaaatatga atgcgcatat ttctcatttg
     7261 catttacaaa caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat
     7321 gagcctgacg acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga
     7381 taatcaatat caagccatca ccaaaccgtc actacttgcc ggttgcatag cactggcact
     7441 attaccttct gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc
     7501 agccacagct ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac
     7561 caaaatgcag atgactcaac ttgatattcc tcagtcggtc actattgtta gccagcagcg
     7621 gatggaagat cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag
     7681 caaaagtcag gcggattccg atcgtgctct ttattattcc cgtggattcc agatcgataa
     7741 ctatatggtt gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact
     7801 ttctgatatg gcactctttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac
     7861 cgggacgggt aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga
     7921 atttaaaggc gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga
     7981 tttacaaagc ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca
     8041 gaataacgac tcatggctgg accgctacaa cagtgaaaag accttcttct cgggcattgt
     8101 cgatgctgat ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga
     8161 tgttaatagc ccaacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag
     8221 ttacgatcgt gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa
     8281 ggtctttatg accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac
     8341 ccactctgaa gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc
     8401 ggatggtatg ctggttgggc catacagtaa ttatggacct ggctttgatt atgtcggcgg
     8461 caccggttgg aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag
     8521 ttatgaattg tttggtcgtc agcacaatct agtgtttggt ggcagttaca gcaaacaaaa
     8581 caatcgttac ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa
     8641 ctttaatggc aatttcccac aaaccgactg gtcaccacag agcctggcac aggacgatac
     8701 cacacatatg aaatcgttat atgctgctac tcgagtcacc cttgccgatc cgctgcatct
     8761 gatcctcggc gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa
     8821 aaaccacacc acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta
     8881 cgccagctat acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct
     8941 ggctccaatc accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg
     9001 tctgaccacc acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg
     9061 tacacctatc cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag
     9121 taaaggggtg gaatttgaac tcaacggcgc aattaccgac aactggcagc tgacatttgg
     9181 cgcaacgcgc tatattgcag aggataacga aggaaacgcc gttaatccta atctgccacg
     9241 caccacggtt aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg
     9301 cggtggtgtt aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt
     9361 ccgcgccgag caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa
     9421 aaacttctcg ttacagggga acgtcaataa cctgttcgac aaaacctacg ataccaacat
     9481 ggaaggttct atcgtctacg gcgcaccgcg taatttcagc attaccggca cgtatcaatt
     9541 ctgaataaca cctgtaaaaa aggcagccat ctggcggcct tagtctcccc aacgtcttac
     9601 ggattagtgg ttacggatgt actcatccat ctcggttttc aggttatcgg atttagtacc
     9661 gaaaatcgcc tgaacaccag aaccagcaac cactacgccc gctgcgccca gtttcttcag
     9721 gccagcctga tccactttag acacatcagc aacgctgacg cgcagacggg taatacatgc
     9781 gtcgaggtta gtaatgtttt ctttaccacc aaatgcagca accagagccg gtgccatttc
     9841 gctggtacct gttgcttttg catcttcagt cgcgtcttca cgacccggcg ttttcagatc
     9901 cagtgcttta atcagcacgc ggaagatggt gtagtaaaca atcgcataac cgataccgac
     9961 gatcgggaac agccacagtt tgctgctgtt accagacaga acgataaagt cgatcagacc
    10021 gtgcgagaac gacgtaccgt cacgcatccc cagaagaata cagattggga atgccaggcc
    10081 tgccagaatc gcgtggatga tgtacaggat cggcgcaacg aacatgaagg agaactcgat
    10141 cggctcggtg ataccggtca ggaacgaggt cagcgccgcg gagatcataa taccgcccac
    10201 tttcgcgcgg ttttctggtt tagcagagtg ccagatagca attgcggcag ctggcagacc
    10261 gtacattttg aacaggaagc caccagacag tttacccgca gtcgggtcac ccgccatata
    10321 acgcggaatg tcgccgtgga aaacctgacc tgctgcgttg gtgtattcac caatctgcat
    10381 ctggaaaggt acgttccaga tgtggtgcag accaaacggc accaggcaac gttcgatgaa
    10441 accgtaaatg ccaaacgcaa ctaccgggtt ctggtaagca gcccactgag agaaggtctg
    10501 gattgcagaa ccaatcggcg gccaaatgaa ggacagcaca acgccagtaa agatggcagc
    10561 caggccagaa atgatcggca caaagcgttt accggcaaag aagccaagat actcaggcag
    10621 cttaatacgg tagaaacggt taaacatgta cgctgcgatc gcaccggaga taatccctcc
    10681 gagtacgcca gtatccgcca ggtgtttaga ggcgatttct tcagcaggta aatgcagtac
    10741 cagtggcgca accacggcca tggttttaac catgatgcca taggcaacaa ctgcggccag
    10801 cgcggatacg ccatcgttat tggtaaagcc gagggcgaca ccgatcgcaa aaatcagtgg
    10861 catgtttgca aagacggaac cgcctgcttc tgccataaca tgcgatacaa cggcgggcag
    10921 ccagctgaaa ttcgcggaac cgacgcccag cagaatacct gcgataggca gtacggatac
    10981 cggcagcatc agcgatttac cgaccttttg caggttagca aatgcattct taaacataat
    11041 tgagagtgct cctgagtatg ggtgcttttt ttacgttctc acgcgtggca aggggggaga
    11101 gcctcgccgt gtacagggca tctaagcgcc ctttatttat tacacagagt aaaataattc
    11161 agtgccgatt tgtttgacgg ctatcacgtt tcaacttcag atcattggag aagttgcaca
    11221 ttttgacaaa acatatgtca caatatttca aacgtggtta ttcaccgccc aattttcggc
    11281 ggtgaacttt actcgtttta gccattaatt acgagcttta aaaaaactca ttcaacggat
    11341 ggattgaagg cgggaagcgt cgatgtgaaa cagacgggcg aagttatcgg tggttacctg
    11401 cgccagttct tcaacggcaa cacctttcaa cacagccatg tattctgcaa cgtcacgaac
    11461 catcgcaggt tgattctctt ttcctcgatg cggtaccggc gcaaggtaag gtgagtcagt
    11521 ttccaccagt aaccgatcca gggggacata acgcgcagca tctcgaagtt gctccgcatt
    11581 acggaaggtc acaatgccgg aaaaggagat gtaaaatccg agatccagta atttacccgc
    11641 cgtttctctg tcctctgtaa aacagtgtag tacgccaccg caatccgtca ctttttcttc
    11701 gcgcagaatt gccagcgtat cggcgcgggc gtcacgggta tggacgataa ccggcttgtt
    11761 cagttcacga ccaatctgga tatgatggat gaaggactct tgctgacgaa ctttagtttc
    11821 cggtgtgtaa taataatcca gcccggtttc acccagcgct acaacaccct cttctgccgc
    11881 cagacggcgt aaatcttcta catcgtaggg atcattctgg ttgagtggat gcacgccaca
    11941 agaaaatacg acgttgtcac gttcgcctac cagatcccgc atatgtaaat aacccggtaa
    12001 tgttgtggcg actgccagac aaaatttcac atcgcgtgcg gcggctttcg ccagaacgtc
    12061 atccacgtcc ttatgcaaag attcataatc cagaccatcg agatggcagt gtgagtcgac
    12121 taaaaacatg atgtctctct taaagatgag gaaccggtag cacaacgccc ggttgcaggt
    12181 aatgctcaat acgcagtaaa agatcggtga tgagaagctc gcggttgatg cctgtgacag
    12241 acattaactg ttcacgaatg tggcaaacat cccccagtat agcctgcagg cgcgagggag
    12301 aaagatggtt tgccagttcg acgacaaggc ccggcacatc aacattggtc acctgcgcag
    12361 caccatgatg gcgttttagc gcatccatca gcaacgttgc cagccagtgt aaacgcgccg
    12421 gagcttgttc atgattaagg gccgccagca gcgaatacca gtcgcccgat ggcacgctat
    12481 atgccaatgc ctgacacaat gtttcacgag cctgccagtt atctccctga aacaacgcca
    12541 gtgccgcgcc aggcgaaccg gcgcttaagc gcaatgcggc aagtaatgca tcctgtgaca
    12601 ttgtcacttc gcgtgaaagc caggtcacgg cgtactgttc cggcggcggc gcaaggtaat
    12661 gtaaccgaca acgactacgt aatgttgcca gtaaacgttc aggctcgcgg gtagccagga
    12721 aaaaccaggt ttctgctggt ggctcttcaa gcgttttcag caatgcgtta gccgcggcgt
    12781 cggtcagtaa ggcagcatcg gttacccaga caactttcgc accacctagg cgtgcgtgct
    12841 cattcagctt ttcggtgacc tcacgtactg catcaatgcc cagcgtattt ttcccttttt
    12901 cgggagccag ggtgtagtaa tcgggatgcg tgccagcctg catcaactga catccacgac
    12961 agtgaccgca acttttgtgg ccctgcggtt gttggcagag taaataacgg ctcagggcgt
    13021 agattaaagc atcatcgccc atgcccggta acgcctgaat gagtagcgca tggtgacctc
    13081 ttccggcctg atagctggct accagttttt cgaaatcagg tcgtaaccat ggataccatc
    13141 tcatgcgtcc aactccttca cccagtgggt cacggtagtg cggattgcat ccatcacggc
    13201 ctccagcggc tgggtggcat caatggtatg aatactttta tcttgtgcag ccagttccag
    13261 atagcgggcg cgggtgcgat taaagaaatc gaaagactct tgctcaatac gatccagctc
    13321 gccgcgcgca cgcgcgcgtt ttaagccaac ttccggggta acatcgagat agagcgttaa
    13381 gtcggggcga aaatccccga gaacagcatc acgcagtgtt gccagcatat gttggtcaat
    13441 accacgtcct ccgccctgat acgcctgagt ggagagatcg tggcgatcgc caatcaccca
    13501 ggtgccgtta gccagagctg gtttgatgac cgtttctacc agttgaacgc gcgcggcata
    13561 aaacatcaga acttcggctt tatcggtaat gacttcatcg cctaccgatt tgatatccag
    13621 caccaggctt cttaactttt cggcaagttg cgtaccgcca ggttcccgag tgaaaaccat
    13681 gtcgcggata cccagttgct caagcgtctc aaccaccaca ttacgcgcgg tagttttgcc
    13741 tgcgccttcc agcccctcaa tgacgatata cttactgcgc atttttttcc ttaagcactt
    13801 tcagataatc ctgcacagac ttgttatggc tggcaagatt ggtattaaac gtgtgaccac
    13861 ctttaccatc ggccacaaaa tagagatacg gcgtttttgc cggatgcgca gcagccttca
    13921 gcgaatccgc ccccggcgta gctatcgcac ccggcggcaa accggtaatg gtataggtgt
    13981 tatacgctgt cggcgtttcc aggtctgcac gagaaagttt gccattataa cgctctccca
    14041 tcccgtaaat cacggttggg tcggtctgca ggcgcatacc aatgcgtaaa cggttgataa
    14101 ataccgaggc aacctgatcg cgttcactgg caacggcggt ttctttttcg ataattgatg
    14161 ccatcgtcac cagctgattt ttatctttat aaggcagacc gtccgcacgc ccttcccagg
    14221 cgctatcgac cgctttcacc attttcttgt gcgctcgctt gagtaacgcg acatcggtgg
    14281 tattggcggt atacatccag gtgtctggcc agaaccaacc ttcaatccac tccgggtttt
    14341 ccagttcaag tgcctgcgct acggtggcgt acttatcgtc actcagcgtg tgcttgatat
    14401 acggagcctc acgcaattgc ttgaggtagt cgctcagacg catcccttct accagtcgca
    14461 gcgggaactg tgcttcttta ccgctttcca gcaatttcag catctcgcgc acggtcatct
    14521 gcggtgtaaa gcggtaagtc ccggctttaa agtgagaaag atccggttcg atacgcagca
    14581 accattgaaa aacccgtggg cgattgatga tcttatcggc ataaagctgt tcaccaagcg
    14641 ccagacgtcc ggtccctggc ttcagggtaa atatcgtctc ttctttgata agcaatttgc
    14701 tgtcggcaag atggcgaacc ttccagacgc ccacaccagc ggcgataccc agtaccacca
    14761 gcaataacaa gataatcaat aacacttttt tcatgactaa ttcgggcgct cacaaagtgg
    14821 ggctaaatat tcatataacg ttgctgacga aaaggagaca tcgccacagg cacgtacggg
    14881 catcactggc attaacgcat tacaaataac catctcatct gcctgcaacg cctcttccag
    14941 agaggcttgc acttcgacaa gctgataaga ggattgtgcc agcaaacgga tacagaattg
    15001 tcgcataatg ccgttaacac ctgcctgatc cagacgcggc gtataaacta cattgccctt
    15061 ccgccagaac aaattagccg cacagcattc cgtaacccac ccttcgctgt caaggaccag
    15121 cgcctcatca gcgtttgtct gctcaagatg agagcggatc aatacttgct caagccgatt
    15181 aagatgttta atacctgcaa gatgaggatt gcgccccagc cgcaccgggc ttagcgccaa
    15241 cgtcatccct tcgttacgca aacggtcgta atgtgcagga taaaccgtaa cggagagaat
    15301 ccgcgttgcc ggtccactgt tcaatgtgct gtaccctcgc ccgccactac cgcgactgat
    15361 cacgactttc agcacaccat tttgctgttc tgctgccagc gttttcatct cttgttcaag
    15421 ctgaggccag aagtcacaga aaatcatcaa ccgctgacaa gcatcctgta gtcgctggat
    15481 atgcgccgat aacaaactga ctttaccgtc gataactctg gcggtggtaa aacaaccatc
    15541 accaaactgc gttgcccgat cgcttactgc cagcgattcc tgcttatgac cgttaattaa
    15601 gaacataccg gctccttatc agagaattgt tagtgtggca gcatgttcac tacggaacaa
    15661 gtcggaataa aagctaagaa aaaaggcccg caagcggacc ttttatatgg gtgggaaatg
    15721 acaacttaga tctttttaaa gatcaaagaa ccattagtgc caccgaagcc gaaggagtta
    15781 cacagcgtgt attccattcc gctgacctga cgtgcttcat gcggtacgaa atccagatcg
    15841 caaccttcat ccgggttatc caggttgatg gttggcggaa cagcctgatc gcgcagcgcc
    15901 aggatggagt agatagattc tactgcaccc gccgcaccta acaggtgacc ggtcatagat
    15961 ttcgtggagc ttaccaacac acggcttgca gcttcaccga agatggtttt caccgcctgc
    16021 gcttcagctt tatcgccagc cggcgtagaa gtaccgtgcg cgttaacgta gccaatctga
    16081 ctcgcttcaa tgcctgcatc acgcagagca tttgccatcg ccagagctgc gcctgcgcca
    16141 ttttctggcg gtgacgtcat atgataagca tcgctgctca taccaaagcc gacgagttca
    16201 gcgtaaattt tcgcaccgcg ttttttcgcg tgttcgtact cttcaagtac cagcataccg
    16261 gcaccatcgc ccagtacgaa gccatcacgc tctttatccc acgggcggct cgccgcttgc
    16321 gggttatcat tgcgggtaga taatgcacgt gccgcgccaa aaccaccaac acccagcggc
    16381 gtactggctt tctctgcgcc acctgcaacc atcacgtcag catcgccata cgcgataata
    16441 cgcgcagcat ggccaatgtt gtgcacgccg gaagtacagg cggtcgcgat agagatgctt
    16501 gggccacgca ggccatacat gatagtcaga tgacctgcca ccatgttcac aatcgttgac
    16561 ggaacgaaga atgggctgat cttacgtgga ccaccgttca tcagagatgt gtggttttct
    16621 tcgatcagtc cgaggccgcc aatcccggag ccaattgcgg caccaatgcg ggttgcgttc
    16681 tcttccgtta tatcaaggcc agaatcctgc atggcctgaa cgccagcgac aattccatat
    16741 tgaatgaagg catccatctt gcgctgttct ttgcgcgaga taatgtcctc acagttaaaa
    16801 tcctttacta agccagcaaa tttcgttgca taggcgctag tatcgaaatg gtcgattagg
    16861 ctgatgccac tctgaccggc aagcagagct ttccaggtag actctacggt attgccgaca
    16921 ggagacaaca tgcccagtcc ggtcacaact acacgacgct tagacacgtt tgtcctccag
    16981 ggagggaaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc
    17041 ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg
    17101 gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt
    17161 accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga agcattgttg
    17221 gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg
    17281 atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg
    17341 tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta
    17401 ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca
    17461 cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg
    17521 ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc caggaatgca
    17581 accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg cgccaggata
    17641 cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca
    17701 acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg
    17761 atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc
    17821 acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct
    17881 tttgacagac ggaaaacaga tgaaaggttg gtttcgataa tatcgttcca ctcttcatct
    17941 ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact
    18001 tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca
    18061 ttcaacatca gacctttgcc gttggcacct aaataatcgc tgatcgcctg agcgccattt
    18121 tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg
    18181 cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatt
    18241 ttcctctttt aaagctcaag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg
    18301 gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg gccgacttca
    18361 tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca ctgaaccggg
    18421 ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc gcatttcaca
    18481 tcaacgttat tcacaacaga aactgttggt gcgttaaagg tgattttcgc taattctact
    18541 gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac
    18601 ggcagagcac gttttgcgcc cgcagcttta caggcagcgc cagcacgctc aaccgcttct
    18661 ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga
    18721 cctgctgcag cttcttcaca cgctttcgca atagacgcat catccagacc gatgattgcc
    18781 gccatagcgc ccgtaccttc cggtacggct tcttgcatga gcttgccgcg catctcaacc
    18841 agacgcaccg catcagcgaa atcaatcaca ccagcgcaaa ccagcgcgga atattccccc
    18901 aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca tacgcggtac
    18961 agcgcaacag aggcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca
    19021 gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca
    19081 aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga
    19141 gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa
    19201 cgaaccagcg cggagcccca ggtgaatcca ccgccaaagg cttcaagcag aaccaactgc
    19261 cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac agaggccgca
    19321 gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatagacat accgagtttt
    19381 ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga
    19441 gaacggtcaa gattatttgc cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc
    19501 gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcaca
    19561 cggtcagcat taggcagcgt cagcaactca ccatagctac cgtcggcatg cagatgggtg
    19621 gagatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa
    19681 ataataatag tcccacgatc ggttggatcg caggtgcgcg ccagtacatc ggaaccgacg
    19741 accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag
    19801 gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccagcatg
    19861 ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg
    19921 atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca
    19981 aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta
    20041 cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc
    20101 acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca
    20161 cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg
    20221 cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag
    20281 gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc
    20341 gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc
    20401 taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct
    20461 cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg
    20521 agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta atgtgacatt
    20581 tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc
    20641 aagatagccg atataattga tagaagggat tgttttaagc accgctgagg catcccgaat
    20701 actgtcgaga ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattggg
    20761 aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca ccaacattgt
    20821 actgtcacaa tcgacgttgg cccctaagtc aaggaccacc gttttgccct tttgctgatg
    20881 tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt
    20941 tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc
    21001 tttcaccagc tccagggcca cgcgcattga actcccacga ctggcgcgga tagcttgcga
    21061 aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg
    21121 ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag
    21181 agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc
    21241 aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc
    21301 cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg
    21361 cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga
    21421 cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg
    21481 tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg
    21541 gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc
    21601 tttaagctgg ctaatacggc aaatgggttt ggcttttgca cttcttcagg cagttcacca
    21661 aagaccatgt ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc
    21721 aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta
    21781 acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga
    21841 ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg
    21901 agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg
    21961 aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca
    22021 acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga
    22081 tcgagagtca ggggtaattt taccttttgc atagggcgcg catattaact ttgtaacgtc
    22141 atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc
    22201 gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg
    22261 catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac
    22321 ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg caaatctctt
    22381 ttgaatgtgc agcaccagag gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt
    22441 tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt
    22501 taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa
    22561 cggaagaaaa tgcctgtctg caattacgca aagccagcgg caatatcgtc accttctata
    22621 ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg gagccttttg
    22681 acgtccattt ccgtcacctg agcgaggcgg agattgataa ttacgtgcgt aaggagcatc
    22741 ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt
    22801 tagagggacg tgaccctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt
    22861 tacgtcggga agggaaaaac ccgctgatgg gatagttgtt tgcgctggat gcggttacgt
    22921 cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa acgccttatc aggccaaccg
    22981 cccattatca ggcttatatg cttgtattag cgcgcgttac gcagcttttg caaacaacgc
    23041 ttcaaacctt catccatcgg cgcttcgata cgcatcacct caccggtccc cggatgggta
    23101 aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc
    23161 tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg acccgcatac
    23221 tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg caccagggtg
    23281 gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc ttcctgactg
    23341 acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc ttgaacgctc
    23401 ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg cattcctttt
    23461 tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctat cagcaacaca
    23521 cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc cgggcgcaac
    23581 gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac cgccgtaccg
    23641 gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc cgccagcgcc
    23701 gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt
    23761 gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg ttttttgttc
    23821 acccgcactt cgcctttacg caaaatacgg taaatcatac tttttggtac gcctttcaat
    23881 tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat agcaacaatt
    23941 tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac aggattcgcg
    24001 ccactcattt ttctatgctt atatttagtt ttgcacctta ttacttcact acgtgatcac
    24061 tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg agtaagcggg
    24121 tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa tgaggccgtt
    24181 tccgtgtcca tccttgttaa aacaagaaat tttacggaat aacccatttt gcccgaccga
    24241 tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc gggctgcggg
    24301 ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt ctgtttcttt
    24361 gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg cctctttgag
    24421 ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt tcacacccgg
    24481 aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt aagttacgat
    24541 gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc ttgtagatgg
    24601 gcagcgtctt tatgacctgg atatcgaaag tccagggcac gagcagaaaa aggcaaacat
    24661 ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa gctgcttttg ttgattacgg
    24721 cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt tccctgctaa
    24781 ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat
    24841 tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca cctttatcag
    24901 tctggcgggt agctatctgg ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg
    24961 ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc
    25021 ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca
    25081 atgggattta agcttccgcc tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg
    25141 cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcat tccgcgatta
    25201 cttacgtcag gacatcggcg aaatccttat cgataacccg aaagtgctcg aactggcacg
    25261 tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac tgtataccgg
    25321 cgagatcccg ctgttcagcc actaccagat tgagtcgcag atcgagtccg ccttccagcg
    25381 tgaagtccgt ctgccgtccg gtggttcgat tgttatcgac agcaccgaag cgttaacggc
    25441 catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa ccgcgtttaa
    25501 caccaacctc gaagctgccg atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg
    25561 cctgattgtt atcgacttca tcgacatgac gcctgtacgc caccagcgtg cggtagaaaa
    25621 ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg
    25681 ctttggcctg ctggaaatgt cccgccagcg cctgagccca tcactgggtg aatccagcca
    25741 tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct
    25801 ctctattctg cgtttgatcg aagaagaagc gctgaaagag aacacccagg aagttcacgc
    25861 cattgttcct gtgccaatcg cttcttatct gctgaatgaa aaacgttctg cggtgaatgc
    25921 gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac
    25981 cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa acgccgacct taagctacat
    26041 gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt tcgctgaacg
    26101 taagcgtcct gaacaacctg cactggcaac ctttgccatg ccggatgtgc cgccagcgcc
    26161 aaccccagct gaacctgccg cgactgtcgt agccccagca ccgaaagctg caacggcaac
    26221 accagcagct cctgcacaac ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct
    26281 gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa
    26341 accggaacgt caacaggatc gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga
    26401 gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg
    26461 taatcgtcgc caggcacagc agcagactgc cgagacgcgt gagagccgtc agcaggctga
    26521 ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg
    26581 ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga atgttgaaga
    26641 gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa
    26701 acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt
    26761 cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc
    26821 acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac cagaacagca
    26881 agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc
    26941 gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc
    27001 aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa
    27061 agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag agcagcgcga
    27121 acaggaagaa gtacaagtgc agccgatggt gactggggtc cctgtcgccg ccgctgtcga
    27181 acctgttgtt agcgcaccgg ttgttgaaga agtggctgaa gttgtagaag cccccgttca
    27241 gattgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga ttgccgccgc
    27301 agtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc aggaagttgc
    27361 agaacacgca gaaccggtgg ttgaaccgca ggaagagacg gcagatattg aagaagttgc
    27421 cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt
    27481 cgtcgctgaa gtcgcagcag aagttgaaac ggtagctgcg gttgaacctg aggtcaccgt
    27541 tgagcataac cacgctacgg tgccgatgac gcgcgctccg gcaccggaat atgttccgga
    27601 tgcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc
    27661 aggtggtcat acggcaacac atcatgcctc tgccgctcct gcgcgtccgc aacctgttga
    27721 gtaataatta gctcaagtaa tcaagccctg gtaactgcca gggctttttt atttcatctt
    27781 tgaatcaaca tattaaaatt catcgaatgg catccttgct aaccaacaat gcaaaatagg
    27841 caaaaaaatg cccgccccgg cgatcccaga gcaggcagac aaaacatacc cagtttcatg
    27901 atatgtttta aagcgaaatt atttgttgag ctggaagagc gacaatccct gcatatcggt
    27961 aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc
    28021 attccagtcc acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct
    28081 acctaatgaa tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacggt
    28141 cagcacattg ttcagcgagt ttttcagtcc gcggttggtt ttatctaacg ccgcagcggc
    28201 ggtttcttta tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcgctatc
    28261 cagcatggca aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttact
    28321 ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc accatcgaac gcgaagcatc
    28381 gacctgttgc ttaatacttt ctgttccgcc gacataatca ccgttagcct cactaaacgg
    28441 cgcagtctct gttttataac cggcaaaaat gtagcgccca ttaccgtcag tggtgttcgc
    28501 cagattcagc aactggtcac gaagcccctg aatatccgta gccagcgagg cccggtcatc
    28561 gtcactcaag gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt
    28621 ggtgacctgg ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgcgc
    28681 cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga acaaccgctt gtgatgcagc
    28741 aatgggatca tctgaagggt taacgactcg cttacccgtc gacatctgtt cgccgtactt
    28801 catccattct gcctgagaat tggtgatacc acgcatgttt tgctggtaca tcatctgggt
    28861 actgaaacgc attttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt
    28921 gccgtctgca gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg
    28981 tactcctcat cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc
    29041 acattacctt gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc
    29101 actaacgagg cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt
    29161 tgcagatcca gcagggcctg accgttgcgg ttatcgctat cacccgcatc tttttcgctc
    29221 gccatcgcta ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt
    29281 actggtttca gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga
    29341 ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg taaaagtggt attgctggca
    29401 aggcgggtga cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc
    29461 acggaggcat cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc
    29521 agaaccgcgg gcttaccgat agtaaagaaa tcttcaccag catcgccgtt agcatcaaat
    29581 ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta
    29641 ttacgcgtct ggtccagctc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta
    29701 ttcagtaatt tctccgggat ctcaatattg cctgccgtcc catcaacata agcgacagtc
    29761 gtacgagaag ggtcagcgct ggaaggaact gccgccagtt gccgcgccgt acttccctga
    29821 accagtgagt aaccattggc caccgtgatg ttataagtgc cgccatcctg aacgctgact
    29881 tctacaccaa caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg
    29941 ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca
    30001 atttgtttag cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt
    30061 ttgtcctggt cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat
    30121 ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt tactcaccag cgtttgcagg
    30181 ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg
    30241 tcgattttcg acatctgctc atagcgggcg gtcaggccgc tactttgcgt ctgcgcagca
    30301 cgtaactggt tggtaataaa cgcatcatac tcacgctgca caccagaaac gtagacgcca
    30361 ttgccaaccc agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg
    30421 cgggtatatc cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc
    30481 tgggccgcgt tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat
    30541 aagccttcaa ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca
    30601 gattatcaat gttcatgctg taggttttgc tcaccttgtc gcttatcgat ttcatctgct
    30661 ggatcatgtt ggtgagttta cgggcatagt gaggatcggt agcatacccc gcgtcctgta
    30721 gggcctgcgc cccctgctcc gcactcgcgg ctgtcgtcac ggcggcgtag cgcgggttac
    30781 gtgttaacag cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaatt
    30841 tcgcttttac tttcttcgct tcgccatttt catattcagt cgtggtgatt tcagtaactg
    30901 gacctttcca gttgccagag gctttgacgc caaacaggtt atagctcggc tcgccgtttt
    30961 cgcggcggat ttgccgttgc ccccaaccag actccagcgc cgcctgagcg aggatcaaat
    31021 gatgtggcac accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga
    31081 atgctttact gtcacccggc agcgaatcat cgtagttacg tggcacggcc ttttgcacca
    31141 gctgcgaaag cgcctgattt tgataacgca ccacagtttc gagcgggaat ttcatcggtg
    31201 ctgctggcgt ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct
    31261 ctgcaagccc cagacctttg cccgccgtca tctgttgggc aatctgctgg tcatacatac
    31321 tggtatacag gcgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca
    31381 tgcttttcaa catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat
    31441 ttgccgccgg atcttcgccc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg
    31501 ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag
    31561 acatcccgca ctttgcattg attgcagtat ggacatcaga tccatcggcg tagcgcccag
    31621 cgcattgagc gcgcgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga
    31681 accgccgctc tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc
    31741 aaacggtgta tctggctggc tgacattggc ctgacgattg actgttactg agagatttcc
    31801 ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg
    31861 cgagttaatc actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc
    31921 tgcaaggaaa cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc
    31981 atctaacgcg gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg
    32041 cgccatgctg aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg
    32101 gctgggcaat tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt
    32161 cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa tattgccctg
    32221 cgccagcgca tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca acgtacctcc
    32281 acgcaagctt ttggcatttc ccatggaaga aaccaccaca tcgatggttt gcccctgacg
    32341 tccaaacgga ggaagtgact ctgtcaccat taccgcagcg acgtttttta gctgcatatt
    32401 ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt
    32461 aaacggcgtc tgggttgtct ggtcaccggt gccatccagc cccaccacca gaccatagcc
    32521 aatcagtgag ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg
    32581 agccgccgtc gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc
    32641 acttacattg gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg
    32701 ttaatgtagc cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg
    32761 gtattgctgc cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta
    32821 ccctgattaa tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc
    32881 tggtcaaccg tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt
    32941 ccgttgaacg tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc
    33001 tgcaaatagc gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc
    33061 gcagaggagc ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca
    33121 atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca
    33181 gactggaaaa tagaaccgtt ggcgacgggc gtcggaccgg gaaccggttg tgcactggtc
    33241 gccccctgca ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac
    33301 aagctggaaa tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat
    33361 cagtaaaccg gtgaacctgc caccggttaa gccttagagt tgcgtcagtt tttgcagcat
    33421 ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat
    33481 attgaccagt tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag
    33541 tcccgcgccg ttcaggcccg gcgtgctttc gttcggtaca ccagaggatt gcgtttcggt
    33601 gtagaggttt tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg tgagattgag
    33661 ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg
    33721 accgatggtg atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc
    33781 accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc tgatgaacca
    33841 tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttgttg
    33901 gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg
    33961 ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga
    34021 atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg
    34081 acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt
    34141 ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattagtg acaaagtgca
    34201 tatgtcttct tgccggatgc ggcgtaaacg ccttatccgg cctacaagtg ctcgcaaaat
    34261 cgataaattg cgattgcccc catagacccg ataaacttgc gcatcgggca acttatactt
    34321 gtcatgtatc ctttcaatta actcatcgac agcagttggt tggcacggcc tgcgttatca
    34381 tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc aatcatgtcg
    34441 ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat cacacgcaag
    34501 gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct taaacgaaaa
    34561 atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt cagacgccct
    34621 actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt gccatcggca
    34681 gcaatagtga tttccgctcc ttccggcaca gcaattggcc ccgcctcgcc tatcacctga
    34741 tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt acgcgtatac
    34801 ccttcgctgc cgtcagcggt ctgcacggcc agccagccat cctgctgcaa cgcgacgtcc
    34861 agcgggcgcg aggtgtaatc cattttgccg ggcgtcatat ctgcgcccgg cgttgacgcc
    34921 gtgaccaacg tgcgcgtggg cagagaaagc ccttccactg gcaccgcgcg taaagcattc
    34981 aactgcgcgc gaaaaccggg cgttgaggca ttggccagat tactggcggt taccgcctgt
    35041 tgattcagtg tctggctggc tgctcccatc gcggtataaa ttgcgtgatc cattgagcta
    35101 tcccgtcagc gattagcgta agttaaccag cgtgttgagg atctggtcct gagttttgat
    35161 ggtctgggcg ttagactgat agttacgctg ggcaacgatc atattgacca gttctttact
    35221 gagatcgaca ttggacgctt ccaacgcacc gttggtcagg gtgccaaagt tgcccgtccc
    35281 ggctgtcccc aacagcgcca cgccagaaga ttgcgtcgca gaccagacgt tgtcgccttc
    35341 ggatgccaga ccttcgttgt tggcaaagtt cgccagtaca atctgcccca gcagttgtgt
    35401 ttgttcgttg gaatagttgc cgacaaccgt accgtcatca ttgatttgat aactcaccag
    35461 atcgcccggt ttgtagccat tctgggtggt tgccacaata ttgttagcgc cggtattttg
    35521 ctgcatggag ttgaggaagc tcaggctaaa cgtggcggga tctgcgccgt taattgcgcc
    35581 agtggtaata ttctctgttg gatttgaggt cagaacgcca ttggcattaa acaccagctt
    35641 cattgcaggc tcggctgtac ctgttggatc actgctatcc tgggtgtaga cctgccagtt
    35701 attatccccg gtcttcacaa agtagacgct catgtcatga gcattaccct gactgtcgaa
    35761 aacagtcacc gaaccttttt tgttatagct atccgcattg ctggcgctga atggcgtaac
    35821 agtaggaagc ggatcactgg aattcaggtt gatctgcatc gacgccgtgg tggtagtttt
    35881 cgctgccatc agggtattcg ggatcgaaat attggtcgga ttcgcccctt gctgaatagt
    35941 cggcggcgta ccggttgccg ggtaacccgt cagctgtaaa ccttgcatat tcaccaggtt
    36001 acggttttca tccagcttaa attgtccgtt acggctgtag aacaccgaac cgttgctgtc
    36061 taccagacgg aaaaaaccgt tctggctgat agcaacgtcc agacctcgcc cggtgttggt
    36121 ggtcgtgcca tcggtaaagt cctgagtgat accggcaact tttaccccca gtcccacttt
    36181 cgaaccggca aacatatcgg caaaagaggc cgtgcctgat ttaaagccgt aggtggcgga
    36241 gttggcgata ttgttgccaa taacatcgag gttggtggcg gcagcgttta atccgctaac
    36301 cgcttgagaa aaggccatga ctgactcctg aaatgtgaag gcttaaatta tctgccgtac
    36361 ttcgtcgagg gtggtggtgc cgtaagtgcc gagatccagc gtattaccgc tgttgccgcg
    36421 gatcacaccc tgcaccagag caaactgcag cggctgggca accagttgtg taccaccgtt
    36481 actggcgcta atcgctacat tgtaagaacc gttcggcgca gtgctgccat cagtcaacgt
    36541 accgtcccag gtgaaactgt ggactccggc ggtcagttca ccaatatcaa tggtgcgcac
    36601 aaccgcgcca tttttatcgg tgatggtggc cgtaactttg tctgccgcct gttgcagctc
    36661 aacaccaaac ggcgtggtcg tggtcacagc cccttcttca ctgccggttc ccgcaagaac
    36721 agtggtgccg gggatcatca cgccgtgacc gatcaggtta ctggcctgta acgactggct
    36781 gttatcaatc tgtccggaaa tagatccgag cgtggtattg agtttttcaa tcccgctgac
    36841 cgtgctgatt tgtgccaatt gcgacgtcag ctcgttgttt tccattggat tggtcgggtc
    36901 ctggtttttc agctgcgcca ccaacaaagt cagaaaactg ctttgtaagt ctgcggcgtt
    36961 gctgcccgtg agcgaactac tgctggtggt actgacgccg gtatttgtcg gatcggtggt
    37021 ggttaccgca atggacatag ctttctcctt tattgaccga gcgtaagggt tttcagcatc
    37081 atgcttttca ccatgttgag cacttcaaca ttggcctgat agctgcgtga cgccgacatg
    37141 gtgttaacca tctccccgac gacatcaacg ttcggcattt ttacgtagcc ctttgcatcc
    37201 gccagcggat tacccggttc ataaaccagt ttgtccgggg cctgactttc tataacatcg
    37261 gcaaccttta cgccgcctgt cgcagcacct ggtgcagcgt taacctggaa taccacctgt
    37321 tttgcccgat atggctgtcc atcgggaccg gtcacgctat cagcattcgc cagattactg
    37381 gccgccacgt tcaagcgctg ggactgggca gttaacgccg acccggcgat atcaaaaata
    37441 ttcagcagtg ccatccgtta atttccgctc tgtaaaacgt tcatcatgcc tttgatttgc
    37501 ccgctcaacg cgctaaggct catctggtat tgcaggctgt tatcggcaaa ctgggtgcgt
    37561 tcgcgatcca tatcgacggt attaccgtca agcgaaggct ggtccggaat acggtattgc
    37621 agttctgcgg taggaggcgt cagcgcctgc gccggaatgt gttgcgttga ggtcatcgtc
    37681 agtgcaacca cactggttgc atcccgtcca cgttgcatga cttttttaag ttcactggca
    37741 aaatcgatat cgcgcgcctg ataaccaggg gtatcggcat tggcgatgtt tgctgccagc
    37801 acttcctgac gctgggcgcg cagattgagc gcctcttgtt gaaaacgtaa ggcggcgtcg
    37861 agcttatcga gcatatctcc tccgcaggta tcaaaattct gccatcagct taaatgcctt
    37921 tacgcgcgcg ttatcggcgg aataaacgca aaatgggtcg ctatttatgc cgttgatgac
    37981 cattgcggac aggtacaatt cacgttgtag aaatggctgg gggcgaaaat gctggcaata
    38041 aaacgtagcg tggcgatcat ctcgatacta ttcactccgt taagtgcggc gagtaatctc
    38101 acatcgcaat tgcaaacctt ttttagcgcc cagctcgcgg gggtaagtga tgaggttcgt
    38161 gtttctattc gtacagcgcc caatctacta ccgccgtgcg agcagccatt gctttcgatg
    38221 agcaataatt cccgcctgtg gggcaatgtg aatgtgttgg cacgctgcgg taacgacaaa
    38281 cgatatttac aggttaatgt acaggccaca ggaaattatg tggttgccgc gatgcccatt
    38341 gtgcggggag gaaagctgga agctggcaat gtcaaactaa aacgcggacg gcaggatacc
    38401 ctgccaccgc gtacggtgct ggatatcaat caacttgttg atgccgttag cctgcgcgat
    38461 ctatcacccg atcaacctat ccagttaacc cagtttcgcc aggcatggcg ggtaaaagcg
    38521 ggacaacgcg tcaacgtgat cgccagcggt aacgggttta gcgccaacgc agaaggtcag
    38581 gcgctgaaca atgcagccgt cacacagaat gcgcgggtgc gcatggtatc gggactggtg
    38641 gtcagcggcg ttgttgatgc agatgggaat attcttataa accagtaagc tgttaaagat
    38701 tacccgtccc ttgccgataa ataagcaaca catgataaaa gcgccctcaa tgaggaataa
    38761 accatgagta ttgatcgcac ttcgcctctg aagcccgtaa gcaccgttca accacgcgaa
    38821 accactgacg cgtcggtaac gaacacccgg gcggcaaaaa caacagcctc caccagcacc
    38881 agtgtgacgt taagcgacgc gcaagcaaaa ctgatgcaac ccggcagcag tgatatcaat
    38941 cttgaacgtg tcgaagcgtt aaaactggcg attcgtaacg gtgaactaaa aatggacacc
    39001 ggcaaaattg ccgatgcgct gatcaacgaa gcgcagcaag acttgcagag taactgatcg
    39061 tatgacacgt cttgcagaga tcctcgacca gatgtccgct gtgcttaacg atctcaaaac
    39121 ggtaatggat caagaacagc aacatctctc tatggggcag atcaacggca gccagttgca
    39181 atggattaca gaacaaaaaa gctcactgct ggcgacgctg gattacctcg aacagttacg
    39241 caggaaagaa cccaatacag caaatagcgt tgatattagt caacgctggc aggaaattac
    39301 tgtgaaaacg cagcaactac gccaaatgaa tcaacataac ggctggttac tggaaggaca
    39361 gattgagcgc aatcaacagg cgctggaaat gttgaaaccg catcaggaac cgacgctata
    39421 tggggcgaac ggtcagacct caacaaccca tcgcggcggt aaaaagattt cgatctgaat
    39481 attatctccg gcctgcactg caggccggaa tgcattgtta caccgtccgg cgggcaaatt
    39541 ctttaacttt gaagcccagt accgccagtg cagcgaagta cgcggcaatc cccgccagca
    39601 cgaccgccat taaacgcagt aagcgccaag gcatggtacc caatgaccac tccggcatga
    39661 tatgtaacat acctaaaagc acgccagaca tcaccagtac cgccaccacc agacgcaaca
    39721 gaaacgccat ccagccgggt tgcggagtaa agattttctg cttacgcaac tgccagtaaa
    39781 gcagcgaagc attcagacac gccgccagac caatagaaag tgacagcccg gcatgtttca
    39841 acggaccaat aaacgccagg ttcatcaatt gcgttaaaat cagcgtaacg atggcaattt
    39901 tcactggcgt tttaatgtcc tggcgggaat aaaagccagg agccaacact ttcactacaa
    39961 tcaggccaat caaacccacc gagtaggcaa ttaacgcccg ctgggtcatc agcgcatcaa
    40021 acgcggtaaa tttaccgtac tggaacagcg aaacggttaa cggacccgaa agaatgccca
    40081 acgcaaccgc actcggcagc gccaacagga aacaaagacg caacccccag tccatcaaac
    40141 ggttgtattc atcatgattg ccactggcaa aacttttcga cagcgacggc agcaaaatgg
    40201 tgccaagcgc cacgcccagc acaccggagg gaaactccat taagcggtcg gcgtaataca
    40261 tccaagacac cgaaccggaa gcaagaaacg aggcaaaaat ggtgttgatg attaaggaga
    40321 tctggctcac agagacgcca aggatcgccg gtcccatctg tttcaccacg cgcattgctc
    40381 cggcatcgtg gaagttaatg cgcggcagga ccagcatgcc gatcttcttc aggtgcggta
    40441 gctgatacac cagctgcagg atgccgccga ccgtaacagc ccacgccagc gccagcaccg
    40501 gtgggttaaa gtacggtgcg gcaaacagcg cgaaaccaat catgctgatg ttaagcagtg
    40561 ttggcgcaaa cgccggaatc gagaagcggt tccacgtatt cagaatcgct cccaccagcg
    40621 acgccaggga gatcagcaag atatagggaa aggtaatctt tagtagctgg ctggtcaggg
    40681 caaatttgtc agctgtgtca gcgaagcctg gcgcggtcac catgatcacc cacggtgcgg
    40741 cgagcatgcc agcgaccgtc acaaccgcca gcgcaagtgt cagcaggcca gaaacataag
    40801 agacaaagac ccgcgtggcg tcttcaccct gcttactttt atattccgcc agaatcggta
    40861 caaatgccag ggaaaatgcc ccttcggcaa agatacggcg taacaagtta ggaagtttaa
    40921 aagcgacgaa aaaggcgtcg gttgccatcc ctgcgccaaa gattctggcg acaattgcgt
    40981 ctcgtgcgaa gccaagcaca cgcgaaaaca tggtcatcga gctgacggcg gccagcgatt
    41041 ttaataaatt catcggtgtt ctaatccaga cccacgggca aaacgcctgc aaggcaggcg
    41101 ctgaaagagg cgaatagtct acctggatta tggtgaattg ctaccgccag atgttacagg
    41161 gtttattcac tcatcgcatc gcgccagatc ttgtcaacga tacgttgcgc cagcactgcc
    41221 tgttcgccgg cggtttgcag aactgtctga ttttgcacac attcaatgaa gtgccgcgca
    41281 cagccgacaa atccacgttg ctcaagagtg ctctgccaac caggaatcgg tttatgcact
    41341 acgccctgcc cgcgctcctc acgccattcg cgcatatccg taatgtcgat gagcgcaccg
    41401 tcagtcacgg cctgcacggt ttcacgctga cttccggcac ggcgatgcat acaggtggtg
    41461 atctgcaaag gatcggccga aaaatggtgc tcggcaaaca gcatttcgcc agcgtcgtta
    41521 gtcagtagcg taccgccatc cagagaggct ttgccgcccg acaaccacag cgcggtatcc
    41581 accacatgca gataatcatc cagcaacgtg aaataaagat cgtgtggccc gacactattg
    41641 ctacgatgtt tatccattct tagcgaagct gcggtagcga gttgcgtttt taactcaccg
    41701 tagagtggtg cgaaacgacg gttaaaaccg accatcaacg tcagtttttt ccgcgccgcc
    41761 agttccacca gccgttcagc atcgcgcaga ttttctgcca gcggtttatc gacacagaca
    41821 tgtacacccg cattgagtaa cgtactgacc acgtcaaagt ggctggcggt gctggaatgc
    41881 acaaaaaccg catcgcaact ggcggcaagg ctggataacg aatcggcata aggaatgcgc
    41941 cagctttcac aaattggcag ggctttcgcg cgcgtaggcg accaggctcc ttgtaacatc
    42001 cagtcagacg ctgccgccag caccggtaac cacgcttttt gcgcaatgcc gcctaatccc
    42061 acaacgccga tacgtaattt tttcacttta atctcccaga tgggccagca acgaatcaag
    42121 acgctgtttc agttctgcca cttcgatttc cagggcttcg acgcgggcct gtaaatcacc
    42181 atcaaccgcg ttcgacatat ccgttaccgc cggctgatcc tctacctcac cactgaaaag
    42241 atgcatgtag cggctttcgc gtttacccgg ttcgcgggcc agacgcacca caaaaggacc
    42301 atcttcgcga tttgccagtt gttccagcgt cgactccact tccgccatat cgctgaattc
    42361 atacattcgc gcggcgcggc tgcgcagttc acccggcgtc tgggcaccac gcaataacaa
    42421 cgtggtgatt aacgccactt ccgctgcgct cagtttcaga tcgccaaatt ctgaattaca
    42481 aaaacgttgt tcatatttgg tgacccgatt accaaagcca ctcaccgtgc gcagataatg
    42541 gcgtttgacc agattatcca gttgctcctg cacttcagat tcactcagat tcatgaccgg
    42601 ttcacggttc gttttctgat tacaggccgt gactacacca ttgactgaga gcgggtattg
    42661 ctccggcgtg gtcacctgct tttccagcaa gcaaccaatc acgcgagctt caagggcagt
    42721 aagttgatat ttcatgagat tctccttagc ggccgggcgt ccagtctggg gtagttaatg
    42781 ccgtcagtac gtgatcgcgc cattgtccat caatcaacag atagtctttc gcatagcctt
    42841 ctttttcaaa acccagtcgc gccagtaaat caccgctgcg tttattgtgc ggcatataat
    42901 tagccataat gcgatgaata tgttgggtgc gctgcatata acgaatggct gcggtcaggg
    42961 cttcaaacat gagtcctttg ccctgccatt tttgcccaat cgaataaccg agatagcagg
    43021 catgaaaaga gccacgaaca acattggaaa aattggcaac gccaataatc tctttttcgt
    43081 ccgggtcgaa taagccaaag tagaaagctg aaccttgttt atgaaattcg ttaatcatcc
    43141 ccagcctggc ctgccagcct gatggataac agtggctttc gtcgcgcact ggctcccagg
    43201 gcttgaggaa atggcgattc tctgcgtaat aatccgcaag acgccaggca tcacgatcat
    43261 gcaccagacg cacgaccagt cggtctgtgg ttaagcgcac ttttggcacg ttactgcgat
    43321 agccaaacat tctataccta ctccttcccg taacgtcttt aatacgctgt aagtattact
    43381 atacctgcgc aaccagaccc tgtgaaaagt ccaaaatgcc attttctgca ttattcacat
    43441 atttgataaa aactctctat caaagcgacg ttttgataaa aaaatattgt ccccgaagtg
    43501 atgggaaaag tcttagcggc aatgcagaat agaaagtgct catcttttat ttcccgggag
    43561 gggaaatgtc ccgcgtatcg caggcgagga acctgggtaa atatttcctg ctcatcgata
    43621 atatgctggt cgtgctgggg ttctttgttg tcttcccgct gatctctatc cgcttcgttg
    43681 atcaaatggg ctgggccgcc gtcatggtcg gtattgctct cggtctacgc cagtttattc
    43741 agcaaggtct gggtattttc ggcggcgcaa ttgccgaccg ctttggtgcc aaaccgatga
    43801 ttgttaccgg tatgctgatg cgcgccgccg gattcgccac aatgggtatc gcccacgaac
    43861 cgtggctatt gtggttttca tgcctgctct cgggactcgg cggcacgttg tttgatccgc
    43921 cgcgttcggc gctggtggtg aaactaatcc gtccacaaca acgttgtcgt tttttctcgc
    43981 tgttgatgat gcaggacagt gccggtgcgg tcattggcgc attgttgggg agctggctgt
    44041 tgcaatacga ctttcgcctg gtctgcgcca caggggccgt tttgtttgtg ctgtgtgcgg
    44101 cgttcaatgc gtggttgtta ccggcatgga aactctccac cgtacgcacg cccgttcgcg
    44161 aaggcatgac ccgcgtgatg cgtgacaagc gttttgtcac ctatgtcctg acgctggcgg
    44221 gttactacat gctggctgta caagtgatgc tgatgctgcc aattatggtc aacgacgtgg
    44281 ctggcgcgcc ctctgccgtt aaatggatgt atgccattga agcgtgtctg tcgttaacgt
    44341 tgctctaccc tatcgcccgc tggagtgaaa agcattttcg tctggaacac cggttgatgg
    44401 ctgggctgtt gataatgtca ttaagcatga tgccggtggg catggtcagc ggtctgcaac
    44461 aacttttcac cctgatttgt ctgttttata tcgggtcgat cattgccgag cctgcgcgtg
    44521 aaaccttaag tgctttgctg gcggacgcaa gagctcgcgg cagctatatg gggtttagcc
    44581 gtctgggtct ggcgattggc ggcgctattg gttatatcgg tggtggctgg ctgtttgacc
    44641 tgggcaaatc ggcgcaccag ccagagcttc cgtggatgat gctgggaatt attggcatct
    44701 tcactttcct tgcgctgggt tggcagttta gtcagaaacg cgcaacgcgt cgtttgctgg
    44761 aacgcgacgc ctgatttgat actcccccct gactcctcca tactgaaagt gaactgacag
    44821 ccaaatggag gagtcacgtg aagctataca tttacgatca ctgcccttac tgcctcaaag
    44881 cccgcatgat tttcggcctg aaaaatatcc ccgtcgaatt acatgttctg ctcaacgacg
    44941 acgcagaaac acccacccgg atggtcggtc aaaaacaggt tcccattctg caaaaagatg
    45001 acagccgcta tatgccagaa agcatggata tcgttcacta tgtcgataaa ctcgacggca
    45061 aaccgttact gaccggcaaa cgttcccctg ccattgaaga gtggctgcgc aaggtcaatg
    45121 gctacgccaa caaactgctg ttgccgcgtt ttgccaaatc ggcatttgat gagttttcta
    45181 ctcccgccgc gcgcaaatat ttcgtcgaca agaaagaggc cagcgcgggt aattttgccg
    45241 acctgctggc ccactctgac ggtctgatta agaatatcag cgatgatttg cgtgcgctgg
    45301 acaaactgat cgtcaaaccg aacgccgtga atggcgaact ttcggaagat gatattcagc
    45361 tatttccgct actgcgtaat ctgacgctgg tagccggaat caactggcca agccgcgttg
    45421 ctgattaccg cgataatatg gcgaaacaga cacaaatcaa tttgttatca tcaatggcga
    45481 tttaatcctg acccgccgcg gcagcctgta ttcgcgggtt gccgccgttt tcagtttttt
    45541 ctgctggcga gtttatcgct ggtgacacat gctgcgttgt gtcaggatat tgacgcagat
    45601 ttcatgtcga ggactaccat gaacaagttt ttatttgccg ctgcattgat cgtcagtggc
    45661 ctgctcgttg gctgtaatca actcacccaa tacaccatca ccgaacaaga aattaaccag
    45721 tcgcttgcga aacataataa tttctcaaaa gatatcggtt tacccggcgt ggctgacgcc
    45781 catattgttc tgacaaactt gatcagccaa attggtcgcg aagagccgaa taaggttacc
    45841 ctaaccggag acgccaatct ggacatgaac tccctgttcg gtagtcagaa agcgaccatg
    45901 aaactgaagc tgaaagcgct gcccgtgttt gataaagaaa aaggtgcgat cttcctgaaa
    45961 gagatggaag tggtcgatgc gacggtacaa ccggaaaaaa tgcaaacggt gatgcaaacg
    46021 ttgcttccct atttgaacca ggcattacgc aattacttta accagcaacc tgcttacgtc
    46081 ctgcgcgaag atggcagtca gggcgaagca atggcgaaaa aactggcgaa aggcattgaa
    46141 gtgaagccag gcgaaattgt cattccgttt acggattaat gacgagggcg cattcgcgcc
    46201 ctttattttt cgtgcaaagg aaaacgtttc cgcttatcct ttgtgtccgg caaaaacatt
    46261 ccttcagccg gagcatagag attaatgact gcaccatccc aggtattaaa gatccgccgc
    46321 ccagacgact ggcaccttca cctccgcgat ggcgacatgt taaaaactgt cgtgccgtat
    46381 accagcgaaa tttatggacg ggctatcgta atgcccaatc tggctccgcc cgtgaccaca
    46441 gttgaggctg ccgtggcgta tcgccaacgt attcttgacg ccgtacctgc cgggcacgat
    46501 ttcaccccat tgataacctg ttatttaaca gattcgctgg atcctaatga gctggagcgc
    46561 ggatttaatg aaggcgtgtt caccgctgca aaactttacc cggcaaacgc aaccactaac
    46621 tccagccacg gcgtgacgtc aattgacgca atcatgccgg tacttgagcg catggaaaaa
    46681 atcggtatgc cgctactggt gcatggtgaa gtgacacatg cagatatcga catttttgat
    46741 cgtgaagcgc gctttataga aagcgtgatg gaacctctgc gccagcgcct gactgcgctg
    46801 aaagtcgttt ttgagcacat caccaccaaa gatgctgccg actatgtccg tgacggaaat
    46861 gaacggctgg ctgccaccat cactccgcag catctgatgt ttaaccgcaa ccatatgctg
    46921 gttggtggcg tgcgtccgca cctgtattgt ttacccatcc tcaaacgcaa tattcaccaa
    46981 caggcattgc gtgaactggt cgccagcggt tttaatcgag tattcctcgg tacggattct
    47041 gcgccacatg cacgtcatcg caaagagagc agttgcggct gcgcgggctg cttcaacgcc
    47101 ccaaccgcgc tgggcagtta cgctaccgtc tttgaagaga tgaatgctct gcagcacttt
    47161 gaagcattct gttctgtaaa cggcccgcag ttctatgggt tgccggtcaa cgacacattc
    47221 atcgaactgg tacgtgaaga gcaacaggtt gctgaaagca tcgcactgac tgatgacacc
    47281 ctggtgccat tcctcgccgg ggaaacggta cgctggtccg ttaaacaata aaaatcgtag
    47341 cttcctgttg tcattaggtt atttaacctg tataaataac cagtatattc aacagggggc
    47401 tattatgcga attgaagtca ccatagcgaa aacttctcca ttgccagctg gggctattga
    47461 cgccctggct ggcgaacttt cccgccgtat tcagtatgcg tttcctgata atgaaggcca
    47521 cgtatcggta cgttatgccg cagcgaataa tttatcggtt attggcgcaa caaaagaaga
    47581 taaacagcgc attagcgaaa ttctccagga aacgtgggaa agcgccgatg actggtttgt
    47641 cagcgaataa tatgcagtga tttttgtttt tgccgggtcg ccccggcttt tttattttac
    47701 ttacgaattt tgagtacaaa atttctacac ttcctcaaaa taattgcaca atatgctaaa
    47761 aaaatatgaa tgacatgctg ttttattgtt catataaatc ttaaaagcag aaatttatct
    47821 gttcccccct ttaaaaattc ggtgaatacc cttacttatt ggtatactga agttgcttca
    47881 gataaaaacg cattgaacct cgaataacgt tgtctagtaa cacgaattag ggggccatga
    47941 tggaaaaaaa taatgaagtc attcagactc atccgctcgt agggtgggac atcagcaccg
    48001 ttgatagcta tgatgcgctg atgttgcgtt tgcactacca gaccccaaat aagtccgagc
    48061 aggaagggac tgaagttggt caaacgctct ggttaaccac tgatgttgcc agacaattta
    48121 tttcgatatt agaagcagga atcgccaaaa ttgaatccgg tgatttccag gtaaacgagt
    48181 atcggcgtca ttaacatgac atgaccatcc acccaaaagg cacctcaccg gtgcctttac
    48241 catttctggg ttgtatatct gcattcagtt aattactctg cttaaaccat gagagcagag
    48301 agaaaaaaat gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg
    48361 ggtattatgc aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat atgccaccgc
    48421 atcaacacgg cagccaccac ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg
    48481 aaaagtatgt cccgctggtc ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca
    48541 acgaagatga tcccattttt gtacgctctg gtgtcattaa cctcggcccg gctgactccg
    48601 catttctcgc caacgtcgcc cacagcgccg aacagtggca actcaacgtt gaaaagctcg
    48661 atgcgcaagg gattatggcc cgctggccag aaatacgcgt cccggacaac tacatcggct
    48721 tatttgagac tgattccggt tttttgcgca gcgaactggc gattaaaacc tggatccaac
    48781 tggcgaagga agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg
    48841 acgatgatgg cgtaactatt gaaacggttg acggtgagta tcaggcgaaa aaagcgattg
    48901 tctgcgcggg aacatgggta aaagacctgc tcccggagca gcctgtccag cccgtacgta
    48961 aagtatttgc ctggtatcag gccgatggcc gctatagcgt gaagaataaa ttcccggcgt
    49021 ttaccggtga actgcccaat ggcgatcaat attatggttt tccggcagaa aacgacgcgt
    49081 tgaagattgg caaacataac ggaggccagg ttatccattc agcggatgaa cgtgttccgt
    49141 ttgcggaagt ggtcagcgat ggttcggaag ccttcccgtt cttgcgcaat gtattgccgg
    49201 gtatcggttg ctgcctgtac ggcgctgcct gcacctatga taattcgcct gacgaagatt
    49261 ttattatcga taccctaccc ggccacgata atacactgct cattaccggc ctgagtgggc
    49321 acggttttaa atttgcgtca gttttagggg aaatagctgc cgattttgcg caagacaaaa
    49381 aaagcgattt tgatttgacg ccattcaggc tttcccgctt ccaataatca tcattacggc
    49441 ctccagaatg aggccgtttt tacaggagta tttaatgcgt cggctgttgc actatctca
//
LOCUS       DAZOZY010000007        46165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZOZY010000007 DAZOZY010000000
VERSION     DAZOZY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.007"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHURQ_000359"
     CDS             complement(<1..716)
                     /locus_tag="ACHURQ_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0822238.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHURQ_000360"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHURQ_000360"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR0822239.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHURQ_000361"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHURQ_000361"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR0822240.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHURQ_000362"
     CDS             complement(3650..4705)
                     /locus_tag="ACHURQ_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0822241.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHURQ_000363"
     CDS             complement(4715..5503)
                     /locus_tag="ACHURQ_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR0822242.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHURQ_000364"
     CDS             complement(5521..6573)
                     /locus_tag="ACHURQ_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR0822243.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="ACHURQ_000365"
     CDS             complement(6604..7446)
                     /locus_tag="ACHURQ_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0822244.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="ACHURQ_000366"
     CDS             complement(7433..8314)
                     /locus_tag="ACHURQ_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR0822245.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="ACHURQ_000367"
     CDS             complement(8335..9645)
                     /locus_tag="ACHURQ_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0822246.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="ACHURQ_000368"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="ACHURQ_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHURQ_000369"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHURQ_000369"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR0822247.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHURQ_000370"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHURQ_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR0822248.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHURQ_000371"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHURQ_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR0822249.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHURQ_000372"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHURQ_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR0822250.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHURQ_000373"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHURQ_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR0822251.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="ACHURQ_000374"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="ACHURQ_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR0822252.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHURQ_000375"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHURQ_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHURQ_000376"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHURQ_000376"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR0822253.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGCTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHURQ_000377"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHURQ_000377"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR0822254.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHURQ_000378"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHURQ_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR0822255.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHURQ_000379"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHURQ_000379"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR0822256.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="ACHURQ_000380"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="ACHURQ_000380"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR0822257.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="ACHURQ_000381"
     CDS             21883..22047
                     /locus_tag="ACHURQ_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822258.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="ACHURQ_000382"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="ACHURQ_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR0822259.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="ACHURQ_000383"
     CDS             23556..23801
                     /locus_tag="ACHURQ_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR0822260.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="ACHURQ_000384"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="ACHURQ_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR0822261.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="ACHURQ_000385"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="ACHURQ_000385"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR0822262.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="ACHURQ_000386"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="ACHURQ_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR0822263.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="ACHURQ_000387"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="ACHURQ_000387"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR0822264.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="ACHURQ_000388"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="ACHURQ_000388"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR0822265.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="ACHURQ_000389"
     CDS             29464..29817
                     /locus_tag="ACHURQ_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR0822266.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTTRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="ACHURQ_000390"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="ACHURQ_000390"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR0822267.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="ACHURQ_000391"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="ACHURQ_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="ACHURQ_000392"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="ACHURQ_000392"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR0822268.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="ACHURQ_000393"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="ACHURQ_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHURQ_000394"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHURQ_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHURQ_000395"
     CDS             complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHURQ_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR0822269.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHURQ_000396"
     CDS             36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHURQ_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR0822270.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="ACHURQ_000397"
     CDS             36881..37630
                     /locus_tag="ACHURQ_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR0822271.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="ACHURQ_000398"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="ACHURQ_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR0822272.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHURQ_000399"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHURQ_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR0822273.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHURQ_000400"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHURQ_000400"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR0822274.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHURQ_000401"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHURQ_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR0822275.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHURQ_000402"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHURQ_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR0822276.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHURQ_000403"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHURQ_000403"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR0822277.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="ACHURQ_000404"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="ACHURQ_000404"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR0822278.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="ACHURQ_000405"
     CDS             complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="ACHURQ_000405"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR0822279.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45559..45654)
                     /gene="ymiC"
                     /locus_tag="ACHURQ_000406"
     CDS             complement(45559..45654)
                     /gene="ymiC"
                     /locus_tag="ACHURQ_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR0822280.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45768..45935)
                     /gene="yciX"
                     /locus_tag="ACHURQ_000407"
     CDS             complement(45768..45935)
                     /gene="yciX"
                     /locus_tag="ACHURQ_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR0822281.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45938..46078)
                     /locus_tag="ACHURQ_000408"
     CDS             complement(45938..46078)
                     /locus_tag="ACHURQ_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR0822282.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        10927 a        12207 c        11622 g        11409 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cacccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgccccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccacgcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcagcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcaatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44701 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44761 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44821 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44881 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44941 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    45001 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    45061 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    45121 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    45181 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45241 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45301 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45361 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45421 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45481 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45541 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45601 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45661 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45721 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45781 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45841 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45901 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45961 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    46021 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    46081 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    46141 caaatcctaa agaaacttta gtcaa
//
LOCUS       DAZOZY010000008        46012 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZOZY010000008 DAZOZY010000000
VERSION     DAZOZY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.008"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(220..432)
                     /gene="rpmE"
                     /locus_tag="ACHURQ_000409"
     CDS             complement(220..432)
                     /gene="rpmE"
                     /locus_tag="ACHURQ_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR0822283.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            635..2833
                     /gene="priA"
                     /locus_tag="ACHURQ_000410"
     CDS             635..2833
                     /gene="priA"
                     /locus_tag="ACHURQ_000410"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR0822284.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2989..4014
                     /gene="cytR"
                     /locus_tag="ACHURQ_000411"
     CDS             2989..4014
                     /gene="cytR"
                     /locus_tag="ACHURQ_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR0822285.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4106..5065
                     /gene="ftsN"
                     /locus_tag="ACHURQ_000412"
     CDS             4106..5065
                     /gene="ftsN"
                     /locus_tag="ACHURQ_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR0822286.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5158..5688
                     /gene="hslV"
                     /locus_tag="ACHURQ_000413"
     CDS             5158..5688
                     /gene="hslV"
                     /locus_tag="ACHURQ_000413"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR0822287.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5698..7029
                     /gene="hslU"
                     /locus_tag="ACHURQ_000414"
     CDS             5698..7029
                     /gene="hslU"
                     /locus_tag="ACHURQ_000414"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR0822288.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7096..8022
                     /gene="menA"
                     /locus_tag="ACHURQ_000415"
     CDS             7096..8022
                     /gene="menA"
                     /locus_tag="ACHURQ_000415"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR0822289.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8115..8600
                     /gene="rraA"
                     /locus_tag="ACHURQ_000416"
     CDS             8115..8600
                     /gene="rraA"
                     /locus_tag="ACHURQ_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR0822290.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8685..8930)
                     /gene="zapB"
                     /locus_tag="ACHURQ_000417"
     CDS             complement(8685..8930)
                     /gene="zapB"
                     /locus_tag="ACHURQ_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR0822291.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9356..10201
                     /gene="glpF"
                     /locus_tag="ACHURQ_000418"
     CDS             9356..10201
                     /gene="glpF"
                     /locus_tag="ACHURQ_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR0822292.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10224..11735
                     /gene="glpK"
                     /locus_tag="ACHURQ_000419"
     CDS             10224..11735
                     /gene="glpK"
                     /locus_tag="ACHURQ_000419"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR0822293.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDCTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11865..12875
                     /gene="glpX"
                     /locus_tag="ACHURQ_000420"
     CDS             11865..12875
                     /gene="glpX"
                     /locus_tag="ACHURQ_000420"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR0822294.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12972..13718
                     /gene="fpr"
                     /locus_tag="ACHURQ_000421"
     CDS             12972..13718
                     /gene="fpr"
                     /locus_tag="ACHURQ_000421"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR0822295.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13723..14151)
                     /gene="uspD"
                     /locus_tag="ACHURQ_000422"
     CDS             complement(13723..14151)
                     /gene="uspD"
                     /locus_tag="ACHURQ_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR0822296.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14178..14477)
                     /locus_tag="ACHURQ_000423"
     CDS             complement(14178..14477)
                     /locus_tag="ACHURQ_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR0822297.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14689..15129)
                     /locus_tag="ACHURQ_000424"
     CDS             complement(14689..15129)
                     /locus_tag="ACHURQ_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0822298.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15230..15829
                     /locus_tag="ACHURQ_000425"
     CDS             15230..15829
                     /locus_tag="ACHURQ_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR0822299.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15937..16704
                     /gene="tpiA"
                     /locus_tag="ACHURQ_000426"
     CDS             15937..16704
                     /gene="tpiA"
                     /locus_tag="ACHURQ_000426"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR0822300.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16759..17514)
                     /gene="cdh"
                     /locus_tag="ACHURQ_000427"
     CDS             complement(16759..17514)
                     /gene="cdh"
                     /locus_tag="ACHURQ_000427"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR0822301.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17621..18610)
                     /gene="sbp"
                     /locus_tag="ACHURQ_000428"
     CDS             complement(17621..18610)
                     /gene="sbp"
                     /locus_tag="ACHURQ_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR0822302.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18930..19892)
                     /gene="pfkA"
                     /locus_tag="ACHURQ_000429"
     CDS             complement(18930..19892)
                     /gene="pfkA"
                     /locus_tag="ACHURQ_000429"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR0822303.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20073..20975)
                     /gene="fieF"
                     /locus_tag="ACHURQ_000430"
     CDS             complement(20073..20975)
                     /gene="fieF"
                     /locus_tag="ACHURQ_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR0822304.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21210..21428)
                     /gene="ogrK"
                     /locus_tag="ACHURQ_000431"
     CDS             complement(21210..21428)
                     /gene="ogrK"
                     /locus_tag="ACHURQ_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR0822305.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21510..22673)
                     /locus_tag="ACHURQ_000432"
     CDS             complement(21510..22673)
                     /locus_tag="ACHURQ_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR0822306.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22673..23152)
                     /locus_tag="ACHURQ_000433"
     CDS             complement(22673..23152)
                     /locus_tag="ACHURQ_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0822307.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23167..25614)
                     /locus_tag="ACHURQ_000434"
     CDS             complement(23167..25614)
                     /locus_tag="ACHURQ_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0822308.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25607..25762)
                     /locus_tag="ACHURQ_000435"
     CDS             complement(25607..25762)
                     /locus_tag="ACHURQ_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR0822309.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25759..26034)
                     /locus_tag="ACHURQ_000436"
     CDS             complement(25759..26034)
                     /locus_tag="ACHURQ_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0822310.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26091..26609)
                     /locus_tag="ACHURQ_000437"
     CDS             complement(26091..26609)
                     /locus_tag="ACHURQ_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR0822311.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26622..27812)
                     /locus_tag="ACHURQ_000438"
     CDS             complement(26622..27812)
                     /locus_tag="ACHURQ_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR0822312.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28223..29641
                     /locus_tag="ACHURQ_000439"
     CDS             28223..29641
                     /locus_tag="ACHURQ_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822313.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29756..30283)
                     /locus_tag="ACHURQ_000440"
     CDS             complement(29756..30283)
                     /locus_tag="ACHURQ_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0822314.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30287..32605)
                     /locus_tag="ACHURQ_000441"
     CDS             complement(30287..32605)
                     /locus_tag="ACHURQ_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR0822315.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32616..33146)
                     /locus_tag="ACHURQ_000442"
     CDS             complement(32616..33146)
                     /locus_tag="ACHURQ_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR0822316.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33139..34047)
                     /locus_tag="ACHURQ_000443"
     CDS             complement(33139..34047)
                     /locus_tag="ACHURQ_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR0822317.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34052..34399)
                     /locus_tag="ACHURQ_000444"
     CDS             complement(34052..34399)
                     /locus_tag="ACHURQ_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR0822318.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34396..35031)
                     /locus_tag="ACHURQ_000445"
     CDS             complement(34396..35031)
                     /locus_tag="ACHURQ_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0822319.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35098..35550)
                     /locus_tag="ACHURQ_000446"
     CDS             complement(35098..35550)
                     /locus_tag="ACHURQ_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR0822320.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35543..36010)
                     /locus_tag="ACHURQ_000447"
     CDS             complement(35543..36010)
                     /locus_tag="ACHURQ_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0822321.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(35973..36218)
                     /gene="lysC"
                     /locus_tag="ACHURQ_000448"
     CDS             complement(35973..36218)
                     /gene="lysC"
                     /locus_tag="ACHURQ_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR0822322.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36118..36543)
                     /gene="lysB"
                     /locus_tag="ACHURQ_000449"
     CDS             complement(36118..36543)
                     /gene="lysB"
                     /locus_tag="ACHURQ_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR0822323.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36531..36956)
                     /locus_tag="ACHURQ_000450"
     CDS             complement(36531..36956)
                     /locus_tag="ACHURQ_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR0822324.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(36971..37468)
                     /locus_tag="ACHURQ_000451"
     CDS             complement(36971..37468)
                     /locus_tag="ACHURQ_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR0822325.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37468..37749)
                     /locus_tag="ACHURQ_000452"
     CDS             complement(37468..37749)
                     /locus_tag="ACHURQ_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0822326.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37753..37956)
                     /locus_tag="ACHURQ_000453"
     CDS             complement(37753..37956)
                     /locus_tag="ACHURQ_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR0822327.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(37956..38465)
                     /locus_tag="ACHURQ_000454"
     CDS             complement(37956..38465)
                     /locus_tag="ACHURQ_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR0822328.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38565..39308)
                     /locus_tag="ACHURQ_000455"
     CDS             complement(38565..39308)
                     /locus_tag="ACHURQ_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR0822329.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39312..40384)
                     /locus_tag="ACHURQ_000456"
                     /pseudo
     CDS             complement(39312..40384)
                     /locus_tag="ACHURQ_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40443..41297)
                     /locus_tag="ACHURQ_000457"
     CDS             complement(40443..41297)
                     /locus_tag="ACHURQ_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR0822330.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41471..43243
                     /locus_tag="ACHURQ_000458"
     CDS             41471..43243
                     /locus_tag="ACHURQ_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR0822331.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43243..44277
                     /locus_tag="ACHURQ_000459"
     CDS             43243..44277
                     /locus_tag="ACHURQ_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0822332.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44649..45791
                     /locus_tag="ACHURQ_000460"
     CDS             44649..45791
                     /locus_tag="ACHURQ_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR0822333.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45793..>46012
                     /locus_tag="ACHURQ_000461"
     CDS             45793..>46012
                     /locus_tag="ACHURQ_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822334.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAIC"
BASE COUNT        10845 a        12357 c        11795 g        11015 t
ORIGIN      
        1 gacaatgcta ccgccatact gcgtcatgtc gccctgacgc gccgccggtt ttccgctcat
       61 cgtaaaatcc ttttgaaata aataagaacc agaagcctag cgccagtcaa caacaggaaa
      121 tgataatgag ttaacacccc ggaatttttc agaatttata aaaggcaaat tacaggcaca
      181 aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc ccgggatgtt
      241 gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct gtttgccagt
      301 gaagaacggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa cggtggagcg
      361 gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt tcgggtgaat
      421 atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc cctaacgcca
      481 gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtacga aggcgcgaaa
      541 tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg caatgtgtat
      601 actaacccac cgaatttcaa gtcaggatga tgctatgccc gttgcccacg ttgccttgcc
      661 cgttccgctt cctcgtacct ttgactatct gctgccagaa ggcatggcgg ttaaagccgg
      721 gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc atcggggttg tggtatcagt
      781 tagcgatgtc agtgagctgc cgcttaacga gctgaaagcg gtagttgaag tgctggacgt
      841 tgagccggta tttactcact ccgtctggcg attactgctc tgggcggcag attactatca
      901 tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc aggggcggcc
      961 tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggccagg cggtggatct
     1021 gaacagcctg aaacgctcac ccaagcaaca acaggcgctg gcggcgttac ggcaagggaa
     1081 aatctggcgc gaccaggttg ccacgctcga atttaatgat gccgcgttgc agacgctgcg
     1141 caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagcgact ggcgaacgaa
     1201 ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa caggccaccg ccgttggcgc
     1261 aattcatagc gcggcagata ctttttctgc ctggctgctg gcgggcgtta ccggttccgg
     1321 taaaacggag gtttatctca gtgtactgga aaacgtgctc gctcagggca aacaggcgct
     1381 ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc gcccgttttc gtgaacggtt
     1441 taatgcgccc gtggaagttc tgcattccgg tctgaacgac agcgagcgtc tttcggcgtg
     1501 gctgaaagcg aaaaatggcg aggcggcgat tgtgatcggc acccgctccg cgctgtttac
     1561 gccgtttaaa aatctcggcg tgattgtcat tgacgaagag cacgacagtt cctacaagca
     1621 gcaggaaggc tggcgctatc atgcccgcga cctggcggtg tatcgtgcgc atagcgagca
     1681 aatcccgatt attcttggct ccgcaacgcc cgcgctggaa acgttgtgca acgtccagca
     1741 gaaaaaatac cgcctgctgc gcctgacgcg ccgggcgggg aatgcgcgtc cggcaattca
     1801 acacgtgctg gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg cgttaatcac
     1861 ccgtatgcgc cagcatttac aggccaacaa tcaggttatc ctctttctta accgccgtgg
     1921 ctttgcgcct gcgctactgt gccacgactg cggctggatt gccgaatgcc cacgttgcga
     1981 tcactactac acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact gtgacagtca
     2041 gcgtccggtg ccgcgccagt gcccttcctg cggttccacg cacctggtac ccgtggggct
     2101 gggcaccgaa cagcttgaac agacgcttgc gccgctgttc cctgatgtgc ccatttctcg
     2161 tatcgaccgc gataccacca gccgcaaagg ggcgctggaa cagcaactgg cggaagtaca
     2221 tcgcggcggt gcgaggattt tgatcggcac acaaatgctg gcgaaaggtc accactttcc
     2281 ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg ctgttttctg ccgattttcg
     2341 ctcggcagag cgtttcgctc agctttacac ccaggtcgcc ggtcgtgccg ggcgtgcggg
     2401 taaacagggc gaagtggtgc tgcaaacgca ccatccggaa catcctctgt tgcaaacgtt
     2461 gctctataaa ggctacgacg cctttgccga acaggcgctg gctgagcgac gcatgatgca
     2521 gctgccgccg tggaccagcc atgtgattgt gcgtgcggaa gatcataaca atcagcatgc
     2581 gccgctgttc ctgcaacaac tgcgtaatct gatcctctcc agcccgctgg cagacgacaa
     2641 attgtgggtt ctcggtccgg ttccggcact ggcacctaaa cgtggcggtc gctggcgctg
     2701 gcagatattg ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta acggtacgct
     2761 ggcactcatc aatacaatac cggattcccg taaggtgaaa tgggtgctgg atgttgatcc
     2821 gattgagggt taaaccgctc acgatgcgag gcggatcgaa aaattcaata ttcatcacac
     2881 ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt tgccgatgtg
     2941 aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt gaaagcgaag
     3001 aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt ctctacagcg
     3061 actgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg taatcgggtt
     3121 gaaaaagcgg cccgggaagt gggttattta ccgcagccta tggggcgcaa cgtcaagcgt
     3181 aatgaatccc gcaccattct ggtgattgtc ccggatatct gcgatccctt ctttagcgaa
     3241 attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct gattggcgac
     3301 tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac caagcaaatt
     3361 gatggcatgt tgctgctggg ttcaaggctg ccgtttgatg ccagcattga ggaacagcgt
     3421 aatctgccgc cgatggtgat ggcgaacgaa ttcgcaccgg aactggagct acctacagtt
     3481 catatcgaca atctgaccgc cgcatttgat gcagtaaatt atctatatga gcaagggcat
     3541 aaacggattg gctgtatagc cggtcccgaa gagatgccgc tgtgtcacta ccgcctgcaa
     3601 ggttacgttc aggcgctgcg tcgctgcggc attatggttg atccgcaata catcgcccgt
     3661 ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc agctgcttga tcttccacaa
     3721 ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact ttctcaggca
     3781 aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga taacatcgac
     3841 ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta cgaaatcggt
     3901 cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg cagtggctct
     3961 cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc ttaaagtaaa
     4021 cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg cgggctgacg
     4081 aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc gcagccaacc
     4141 ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc tgcctgcggt
     4201 ttctcccgct atggtcgcta ttgctgccgc cgtgcttgtg acctttatcg gtggtctgta
     4261 cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga aagtgaccgg
     4321 taacggacta ccaccaaaac cagaagaacg ctggcgctac attaaagagc tggaaagtcg
     4381 ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga aaacgccgga
     4441 gcaactgaca ccagaacaac gtcagcttct tgaacaaatg caggctgata tgcgccagca
     4501 gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc gtcagcaaac
     4561 gctacaacgc cagcgtcagg cgcagcaatt agcggagcag caacgcctgg ctcagcagtc
     4621 gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg cgccggtaca
     4681 ggcgcaaccg cgccagtcta aaccggcttc tactcagcag ccgtaccagg atctgctgca
     4741 aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa gctgcgccgg ttgctcgtgc
     4801 cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga tggtgcagtg
     4861 cggttcgttc agaggcgcgg aacaggcaga gacggtacgt gctcagctgg cgttcgaagg
     4921 atttgactcg aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg gcccggtgaa
     4981 aggcaaagag aacgcagaca gcaccctcaa tcggttgaag atggcgggtc atacaaactg
     5041 cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat aattgcatta
     5101 tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg tcagctcgtg
     5161 acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga tggtcaggcc
     5221 acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct gtacaacgac
     5281 aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt cgaactgttt
     5341 gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga gctggcaaaa
     5401 gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt cgcggatgaa
     5461 actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa cgatcttatt
     5521 gctatcggct ccggcggccc ttacgcccag gctgcggcgc gcgcgctgtt agaaaacact
     5581 gaacttagcg cccgtgaaat tgctgaaaag gcgttggata ttgcaggcga catttgcatc
     5641 tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg atctcccatg
     5701 tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat cggccaggac
     5761 aacgccaagc gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat gcagctcaac
     5821 gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc gaccggtgtc
     5881 ggtaaaactg aaatcgcccg tcgtctggct aagctggcga atgcgccgtt catcaaagtt
     5941 gaagcgacca aattcaccga agtgggctat gtcggtaagg aagtggattc tattattcgc
     6001 gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa aaaccgttat
     6061 cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc tgatcccacc tgctaaaaac
     6121 aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc attccgcaaa
     6181 aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc cgcagcaccg
     6241 atgggcgttg aaattatggc tcctccaggc atggaagaga tgaccagcca gctgcagtcc
     6301 atgttccaga acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat caaagacgcc
     6361 atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga gctgaagcaa
     6421 gacgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat cgacaaaatc
     6481 tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggcgttca gcgtgacctg
     6541 ctgccgctgg tagaaggttg caccgtttcc accaaacacg ggatggtcaa aactgaccac
     6601 attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct gatcccggaa
     6661 ctgcaaggtc ggctgccaat ccgcgttgaa ctgcaggcac tgaccaccag cgacttcgag
     6721 cgtattctga ccgagccgaa tgcctctatc accgtgcagt acaaagcact gatggcgact
     6781 gaaggcgtaa atatcgagtt taccgactcc ggtattaagc gcatcgcgga agcggcatgg
     6841 caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacacactgt tctggagcgt
     6901 ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaaatat cactattgac
     6961 gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct gagccgtttt
     7021 atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa ggccccattt
     7081 ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg gctggaaagt
     7141 ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac ggcgctggca
     7201 tggtggcaag gtcacttcga tccgctggtc gccctgctgg tactaattac cgccgggcta
     7261 ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg cagcgataaa
     7321 cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca gcaagagatg
     7381 aaacgggcgc tcattattac cgtcgtgctc atctgtctct ccgggctggc actggttgca
     7441 gtggcatgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg gttgtcgatc
     7501 attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg tctgggtgat
     7561 atttccgtac tggttttctt tggctggttg agcgtcatgg ggagctggta tttacaggct
     7621 catacattga ttccggcact gatccttccg gcgaccgcct gtggcctgct ggcaacggca
     7681 gtactgaaca ttaataacct gcgtgatatc aatagcgacc gcgaaaatgg caaaaacacg
     7741 ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct gctgatgggc
     7801 tctctggtat gtctggcgct gtttaatctc ttttcgctgc atagcctgtg gggctggctg
     7861 ttcctgctgg cggcaccatt actggtgaag caagcccgtt atgtgatgcg ggaaatggac
     7921 ccggtggcga tgcgaccaat gctggaacgt actgtcaagg gagcgttact gactaacctg
     7981 ctgtttgttt tagggatatt cctaagccag tgggcagcat aactgacaaa tatcaattaa
     8041 caattgatga ttttgccaac agcctacata gcgcgatata ctgaaaattc tcgcagcaac
     8101 tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat caagaagatg
     8161 ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt ggcggacaaa
     8221 taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc gaacagaatg
     8281 gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca ctggtcgatg
     8341 ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggttatt tacggcgcgg
     8401 tgcgtcaggt agatgaccta gaagagttgg atatcggcat ccaggcgatg gcggcaattc
     8461 cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat tttggcggtg
     8521 taaccttctt ctccggcgac catctttatg ccgacaatac cgggattatt ctttcagaag
     8581 atccgctgga tattgaatga taagaaaggc accgcaaggt gccttttttc tgcgttacct
     8641 gttggcctac acagtaaaga aattacgcga aagatgaagc gtaatcagac ctcttccatg
     8701 cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt cagatggttg
     8761 ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg cgacagtgag
     8821 ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt atcaatcgcc
     8881 tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat acctctcctg
     8941 aattgcaagg cgttgatgga taaaaaatcc tcgtcccgat taccggtgac gccttaataa
     9001 atacgagcgc actttagtta gctccgattg tatgaagccg cgccatcgct gtccagcggc
     9061 acgccttgca gattacggtt tgccacactt ttcatccttc tcctggtgac ataatccaca
     9121 tcaatcgaaa atgttaataa atttgttgcg cgaatgatct aacaaacatg catcatgtac
     9181 aatcagatgg aataaattgc gcgataacgc tcattttatg acgaagcaca cacattttaa
     9241 gttcgatatt tctcgttttt gctcgttaac gataagttta cagcatgcct acaagcatcg
     9301 tggaggtccg tgactttcac gcatacaaca aacattaact cttcaggatc cgattatgag
     9361 tcaaacatca accttgaaag gccagtgcat tgctgaattc ctcggtaccg ggttgttgat
     9421 ttttttcggt gtgggttgcg ttgcagcact aaaagtcgct ggtgcgtctt ttggtcagtg
     9481 ggaaatcagt gtcatttggg gactgggggt ggcaatggcc atctacctga ccgcaggggt
     9541 ttccggcgcg catcttaatc ccgctgttac cattgcattg tggctgtttg cctgtttcga
     9601 caagcgcaaa gttattcctt ttatcgtttc acaagttgcc ggcgctttct gtgctgcggc
     9661 tttagtttac gggctttact acaatttatt tttcgacttc gagcagactc atcacattgt
     9721 tcgcggcagt gttgaaagtg ttgatctggc aggcactttc tctacttacc ctaatcctca
     9781 tatcaatttt gtgcaggctt tcgcagttga gatggtgatt accgctattc tgatggggct
     9841 gatcctggcg ttaacggacg atggcaacgg tgtaccacgc ggccctttgg ctcccttgct
     9901 gattggtcta ctgattgcgg tcattggcgc atctatgggc ccattgacgg gttttgccat
     9961 gaacccagcg cgtgacttcg gtccgaaagt ctttgcctgg ctggcgggct ggggcaatgt
    10021 cgcctttacc ggcggcagag acattcctta cttcctggtg ccgctttttg gccctatcgt
    10081 tggcgcgatt gtaggtgcat ttgcctaccg caaactgatt ggtcgccatt tgccttgcga
    10141 tatctgtgtt gtggaagaaa aggagaccac aactccttca gaacaaaaag cttcgctgta
    10201 atatgactac gggacaatta aacatgactg aaaaaaaata tatcgttgcg ctcgaccagg
    10261 gcaccaccag ctcccgcgcg gtcgtaatgg atcacgatgc caatatcatt agcgtgtcgc
    10321 agcgcgaatt tgagcaaatc tacccaaaac caggctgggt agaacacgac ccaatggaaa
    10381 tctgggccac ccaaagctcc acgctggtag aagtgctggc gaaagccgat atcagttccg
    10441 atcaaattgc agctatcggt attacgaacc agcgtgaaac cactattgtc tgggaaaaag
    10501 aaaccggcaa gcctatctat aacgccattg tctggcagtg ccgtcgtacc gcagaaatct
    10561 gcgagcattt aaaacgtgac ggtttagaag attatatccg cagcaatacc ggtctggtga
    10621 ttgacccgta cttttctggc accaaagtga agtggatcct cgaccatgtg gaaggctctc
    10681 gcgagcgtgc gcgtcgtggt gaattgctgt ttggtacggt tgatacgtgg cttatctgga
    10741 aaatgactca gggccgtgtc catgtgaccg attgcaccaa cgcctctcgt accatgttgt
    10801 tcaacatcca taccctggac tgggacgaca aaatgctgga agtgctggat attccgcgcg
    10861 agatgctgcc agaagtgcgt cgttcttccg aagtgtacgg tcagactaac attggcggca
    10921 aaggcggcac gcgtattcca atctccggga tcgccggtga ccagcaggcc gcgctgtttg
    10981 gtcagttgtg cgtgaaagaa gggatggcga agaacaccta tggcactggc tgctttatgc
    11041 tgatgaacac cggcgagaaa gcggtgaaat cagaaaacgg cctgctgacc accatcgcct
    11101 gcggcccgac tggcgaagtg aactatgcgc tggaaggtgc ggtgtttatg gcaggcgcat
    11161 ccattcagtg gctgcgcgat gagatgaagt tgattaacga cgcctacgat tccgaatatt
    11221 tcgccaccaa agtgcaaaac accaatggtg tgtatgtggt tccggccttt accgggctgg
    11281 gtgcgccgta ctgggacccg tatgcgcgcg gggcgatttt cggtctgact cgtggggtga
    11341 acgctaacca cattatacgc gcgacgctgg agtctattgc ttatcagacg cgtgacgtgc
    11401 tggaagcgat gcaggccgac tctggtatcc gtctgcacgc cctgcgcgtg gatggtggcg
    11461 cagtagcaaa caatttcctg atgcagttcc agtccgatat tctcggcacc cgcgttgagc
    11521 gcccggaagt tcgcgaagtg actgcattgg gcgcggccta tcttgctggt ctggcggtcg
    11581 gtttctggca gaacctcgac gagctgcaag agaaagcggt gattgagcgc gagttccgtc
    11641 caggcatcga aaccactgag cgtaattacc gttacgcagg ctggaaaaaa gcggttaaac
    11701 gcgcgatggc gtgggaagaa cacgacgtaa tgtaaatgcc ggatgaagcg tttatgccgc
    11761 atccggtagt cccgaaacgt gcgggggcaa ccccgcacac atcaataatc cctcccttcc
    11821 cctgtgctac acttcgcgcc attccttact gcttagagtt tgctatgaga cgagaacttg
    11881 ccatcgaatt ttcccgcgtc accgaatctg cggcgctggc tggctacaaa tggttaggac
    11941 gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc catgcgtatt atgctcaacc
    12001 aggtcaatat tgacggcacc atcgtcattg gtgaaggtga aatcgacgaa gcaccgatgc
    12061 tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc ggtagatatt gctgttgatc
    12121 cgattgaagg cacgcgcatg acggcgatgg gccaggctaa cgcgctggcg gtgctggcag
    12181 taggcgataa aggctgcttc ctcaatgcgc cggatatgta tatggagaag ctgatcgtcg
    12241 gaccgggagc caaaggcacc attgatttga acctgccgct ggcggataac ctgcgcaatg
    12301 ttgcggcggc gctcggcaaa ccgttgagcg aactgacggt aacaattctg gctaaaccac
    12361 gccacgatgc cgttatcgct gaaatgcaac aactcggcgt acgcgtattt gctattccgg
    12421 atggcgacgt tgcggcctca attctcacct gtatgccaga cagcgaagtt gacgtgctgt
    12481 acggtattgg tggcgcgccg gaaggcgtag tttctgcggc ggtgatccgc gcattagatg
    12541 gcgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa aggcgacaac gaagagaatc
    12601 gtcgcattgg cgagcaggag ctggcacgct gcaaagcgat gggcatcgaa gccggtaaag
    12661 tattgcgcct gggcgatatg gcgcgcagcg ataacgtcat cttctctgcc accggtatta
    12721 ccaaaggcga tctgctggaa ggcattagcc gcaaaggcaa tatcgcgact accgaaacgc
    12781 tgctgatccg cggcaagtca cgcaccattc gccgcattca gtctatccac tatctggatc
    12841 gcaaggaccc ggaaatgcag gtgcacatcc tctgattgat ttgatcgatt gagccttcca
    12901 gtccttcggg actggaattt ttttgttcgg agaacgaaga taaggcaagt caatcaaaac
    12961 aggagaaaaa catggctgat tgggtaacag gcaaagtcac taaagtgcag aactggaccg
    13021 acgccctgtt tagtctcacc gttcacgccc ccgtgcttcc gtttaccgcc gggcaattca
    13081 ccaagcttgg ccttgaaatc gacggcgaac gcgtccagcg cgcctactcc tatgttaact
    13141 cgcccgataa tcccgatctg gagttttacc tggtcaccgt ccccgatggc aaattaagcc
    13201 cacgactggc ggcactgaaa ccaggcgatg aagtgcaggt ggttagcgaa gcagctggct
    13261 tctttgttct ggaggaagta ccagattgcg aaacgctatg gatgctggca accggtacag
    13321 cgattggccc ttatttatcg attctgcaac taggcaaaga tttagatcgc ttcaaaaatc
    13381 tggtcctggt tcacgccgca cgttatgccg ccgacttaag ctatttgcca ctgatgcaag
    13441 aactggaaaa acgctacgaa ggcaaactgc gcattcagac ggtggtcagt cgggaaacgg
    13501 cagcggggtc gctcaccgga cggataccgg cattaattga aagtggggaa ctggaaagcg
    13561 cgattggcct gccgatgaat aaagaaacca gccatgtgat gctgtgcggc aatccacaga
    13621 tggtgcgcga tacacaacag ttgctgaaag agacccggca gatgacgaaa catttacgtc
    13681 gccgaccggg ccatatgaca gcggagcatt actggtaagc ggttacttat cgataaacgg
    13741 cacgatgagc aaatccgcac tcatcttatt gatcatcccg cgatatgccg gcatcaaacg
    13801 gttgataaat gagtggtgat gaccacagac aaggaggtcg cactgctctt tttgcataat
    13861 ttccagcagt gtttccggca tttctccgcg ttcaatacgc agttttgtct tcggccattg
    13921 aatatttttc gtcagtttat acagcttgtt atccgactta ttcttcaaca attgaagaat
    13981 atcttctgtt gcagggaagt agatacccgg atacaactcg cttaagccat catcaatatg
    14041 aattaacgtc aggtgagcgt cattatgtct ggcgagctcc agggctttat tcaccagtaa
    14101 ggcatcttct tcattcccgg aaattgccac gccaatgtgt ttataagcca tgtttaactc
    14161 cttctaaagc aactccatca agctaacgaa cgcagtgata actcaaaaat aatcatctcg
    14221 gcctggcatg agaaagtgaa ggccgcatca agctcaactt gcccctccag atccttaatc
    14281 ttataggcca cgctggcccc ttcatctgca gaggatgcag cggcggcaag ctctttcagt
    14341 ttggtcataa aagactctgc ttcttcgcgg gtggcaaaaa agcgcgaaaa cttactggta
    14401 cagttgtcgt tatcaatcac cgtaccgata tcaatcgcac atcctttagt actgcattta
    14461 tctacgacat ctttcatagg gcacctctgt atattcgcct ctctgtcgat tacgacttaa
    14521 gaataatgcg ccgttaaggg aagaacaaac aagtaatgat cttatggatt accatttttt
    14581 ttgatattgc ttaaggtgaa gagttcagaa ggattttccg ggtattgtca tttgcatcgt
    14641 ggaaataaca atcccggaaa aaaatagcag taagagatat attaaaggtt aattacttga
    14701 tttattgtcg gctttatact tcacatcctg agtatcttta ccatatttat tttcgccttg
    14761 tgtgccaaca aacgcaccaa gatcgataag catcatcacc agaatcaacg tcgggacaaa
    14821 acgccccacc gcccattgcc agacacccgg taaaatcgcc cagttacccg ccagcagcat
    14881 ccacgccaca atcatcagaa atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt
    14941 aacagttact gccgctgttg gccaaagcaa gcacacaagg caaaacgccg cggtctgaat
    15001 atcgagtaaa tttttacccg ccagtgagaa aagcaccagc atgcctgcga accacaggcc
    15061 tatccaaatc cagaaatcac ggcgtccaat acgcccttta aatgagaata accattgctg
    15121 tatggtcatg taagttcctt gatggttgtc ttttccagga ttctaccctt ttgacaaggg
    15181 cgacagatat cgttttaatc ggagccagtc ataacaaaag gtactgtcaa tgaagccagg
    15241 gtgtacgctg ttttttctct tatgttctgc attaaccgtt acaacaacgg cgcatgcaca
    15301 aacaccagat acggcaacga ccgcgcctta tctgctggct ggagccccta ctttcgatct
    15361 ctccatcagc cagtttcgag aagactttaa cagccagaat cctagcctgc cactgaacga
    15421 atttcgtgcc atcgacagca gtcccgacaa agccaatctc actcgtgctg caagtaaaat
    15481 taacgagaac ttgtatgctt ctacagcgct ggagcgcggt accttaaaaa tcaaaagcat
    15541 tcaaatgacc tggctaccca tccaggggcc agagcaaaaa gcagcgaaag cgaaagctca
    15601 ggaatacatg gcagcagtga tccgcacact caccccatta atgaccaaaa cacaaagcca
    15661 gaaaaaactg cagtcgctac taacggcggg gaaaaacaaa cgttattaca ccgagacaga
    15721 aggtgcactg cgttatgttg tcgcggacaa cggcgaaaag gggctgacct tcgctgttga
    15781 accgattaag ctggcgctat ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa
    15841 gcctttgtgc cgatgaatct ctatactgtt tcacagacct gctgccctgc ggggcggcca
    15901 tcttccttta ttcgcttata agcgtggaga attaaaatgc gacatccttt agtgatgggt
    15961 aactggaaac tgaacggcag ccgccacatg gttcacgagc tggtttctaa cctgcgtaaa
    16021 gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat
    16081 atggcgaagc gcgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg
    16141 aacctgtccg gcgcattcac cggtgaaact tctgccgcta tgctgaaaga catcggcgct
    16201 cagtacatca tcatcggcca ctctgaacgt cgtacttacc acaaagagtc tgacgaactg
    16261 atcgcgaaaa aattcgcggt gctgaaagag cagggcctga ctccggttct gtgcatcggt
    16321 gaaaccgaag ctgaaaacga agcgggcaaa actgaagaag tttgcgcacg tcagatcgac
    16381 gcggtactga aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct
    16441 gtatgggcaa tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa
    16501 ttcatccgtg accacatcgc taaagttgac gctaacatcg ctgaacaagt gatcattcag
    16561 tacggcggct ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agatatcgac
    16621 ggcgcgctgg ttggcggtgc ttctctgaaa gctgacgctt tcgcagtgat cgttaaagct
    16681 gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg atttcatatc cggcacttac
    16741 tttccttaac tcttcgcctt aacgcaaaat ctcacactga tgatcctgaa tttcctcggc
    16801 tgaagcacgg ttaagcgtca gtagatttcg ttgtgtcgcc agcaatacaa atgagttatc
    16861 actctgccgt accatcgcca gcccgtagct tcccatatgt tcccgcgcct caggtacttc
    16921 ttctgccagc atcataaatg ggctgcgttg taccagttcg ctttccgtta cccgacgcgc
    16981 caggtattca tgcccgcgca aaccacctgg cagtggcaac cagcggctgc tgatgttcgc
    17041 cagattgtta tccagttgtt cgcgcacatc aggacgaata caagagatat gaatatgaaa
    17101 atggttttgc gtacgcccgg tgcgggagtt gatcgccaaa gaaaccgcgc gatcgggaac
    17161 cggctggccg tattttttgc tcataaaatc acgcgcctgc caggccaacc aaaagaagtt
    17221 cggcgttgaa ggatcggtca acaaaggact ttcagtaccg ttaatacgat acgttggcat
    17281 caacagatat tgcagtggac cattaagatc ttttaaaacc acgtatccgg cattgggttt
    17341 gacttccgca catggcgaag gattttgatt ttgctgctga ttgggcaaac attcctcaag
    17401 gacaatctta cgtaatgtat ccgactcttc accggttaat ttccagtaac caataccggc
    17461 agccacaacg gcgataacta tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt
    17521 ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc gtgacaaata aaaaacccgg
    17581 tcgcgagaaa ccaccgggtt tgataattat cctggggaaa tcagcgtttg ctgatctgat
    17641 cgaacgtacc gccgttagca aaatgctctt tttgcgcttt cgtccagccg ccgaactctt
    17701 catcaatggt gaataacttc agctttggaa acgcattttc gtactttttc gctacttcag
    17761 cgtcgcgcgg acggtagtag tttttcgcgg caatttcctg accttctggc gagtagagat
    17821 atttcaggta ggcttccgcc acctctttgg tgcctttttt ctcgaccact ttatcgacca
    17881 ccgacacggt tggctctgcg aggatagact cactcggcgt gacgatttcg aatttatctt
    17941 tccccagttc attcgctgcc agcagagctt cgttttccca ggcgatcagt acatcgccaa
    18001 tcccgcgctc gacaaaggtg ttagtggagc cgcgcgcgcc agaatccaga acttcgacgt
    18061 ttttatacag tgcccgaaca aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca
    18121 gcgcgtagcc ccaggcagcc aggtagttcc agcgtgcgcc accagagctt ttcggattag
    18181 gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca gtcatggatc tgcttcggat
    18241 tgcccttacg caccaggaaa acaatggtgg aagtgtacgg tgcggagtta tccggcagac
    18301 gtttgatcca ctctttatca atccgcccgc gttccgcaat cgcgtccacg tcataggcca
    18361 gagccagcgt gacaacatca gcttcaatac cgttgattac cgacgtcgct tgtttacctg
    18421 aaccaccgtg tgactgacga atcaccacgt tatcaccagt ttgctgtttc cagtgggcgc
    18481 tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa
    18541 gctgaatatc ctttgccata acgctggttg ccgccagcaa aaatgttaac cctacgcccc
    18601 acttgttcat cgcccgactc tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt
    18661 taaccaaaca ttaaagaata aaaaaagatt ggctataact tgcgggtata tgttgaggga
    18721 ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg
    18781 atgcggcgcg aacgccttat ccggcctaca aaagtttgca aattcaataa attgcagaat
    18841 tcatgtaggc ctgataagcg aagcgcatca ggcatttttg cttctgtcat cggtttcagg
    18901 ctaaaggaat ctgccttttt ccgaaatcat taatacagtt ttttcgcgca gtccagccag
    18961 tcacctttga acggacgctt catgttttcg atagcgtcga tgatgtcgtg gtgaaccagc
    19021 tgttcgttct ggatacctac acaacgaccg ccgtaacctg ccagcagcag atcgatagcg
    19081 taagcgccca tacgggaagc cagaatacgg tcgtaaggca ccggagaacc accgcgctgg
    19141 atgtggccca gcacagttgc gcgggtttca cgaccggttt ctttctcgat gaaatgcgcc
    19201 agttcgtcaa catcacacat atgttcggta atcgccacga tcgcgtgttt tttacctttc
    19261 gcgatacccg ctttgatttc gtttaccagg tcttcacggc tgaattcaac ttccggaacc
    19321 acaacgaatt cacagccacc ggcaatggcc gcagccaacg tcagatcgcc acaataacgg
    19381 cccatcactt ccaccacgga aatacgctgg tgagaagaag aggtgtcacg cagacggtcg
    19441 atcgcttcta caacggtgct cagcgcagtg aagaaaccga tagtgtagtc agtgcctttg
    19501 atgtcgttgt cgatagtgcc cggcagaccg atgcacggga agcccatttc ggtcagacgc
    19561 attgcaccca tgtaggaacc gtcaccgccg ataaccacca gcgcgtcgat accacgtttt
    19621 ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc ggaattccgg gaagcgcgca
    19681 gaaccgagga acgtaccgcc acggttgatc atgtcagaaa cgctgtaacg gtctagctgt
    19741 accatacggt cttcatacag acccagatag ccgtcataaa tgcccattac ttccagacct
    19801 tctgtcagcg cagaacgaac aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg
    19861 ccgcttgtca acacaccgat tttcttaatc atgactacct ctgaactttg gaatgcaaaa
    19921 taaaatctgt tgccggaagt cttcttgcac atcgaagtga tccaacgaat gtgcaaatag
    19981 tataacaatc acttcctgct gaattgattc aggtcaggcc aaatggcggt attttataca
    20041 caaaatgcgg gtctggctct cttttatact gattatgaaa gcatagaccg tttaccctcc
    20101 ctgggtacga cggaacaggg gtcctgatgg ataattacat ccgatcccgg aaaacgccgt
    20161 aaaatagcct gctctacctg atccgccacc atatgtgcct gaaccaaagg cagagagtct
    20221 tccatttcca aatgaatctg aataaagcgg gtcggccctg actgccgcgt gcgaagatcg
    20281 tgagcgccgc taacacccgg ccaggaagtc acgatatcaa taatttcttg ccgttcctca
    20341 tcaggcaatg cgcgatccag taatgactgt accgcctcat atcccatgcg taacgcgcta
    20401 tataaaatat agatgccgat tcccaatgca aacagagcat cggcgcgatg ccagccgtac
    20461 caggacaacc ccagcgccag cagaattgcg ccgttcatca taacatcaga ctggtaatgt
    20521 agcatatcag cccgcaccgc ctggctttgc gtccggcgca ccacccaccg ctgaaacgag
    20581 acaaggataa tcgtacaaat tagcgccaca attgtcacga taaccccgac gcctggatct
    20641 gtcatcggtg ttggagatac cagatgttga atacccgtca aaaacaggaa tagtgccgaa
    20701 ccggagataa acatactttg cgccagcgcc gcgagggact cagctttacc gtgaccaaac
    20761 gagtgattat cgtcggcagg ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac
    20821 gcgccgatat ccaccagcga atccaccagc gcggcgagaa tactcaccga cccggtatac
    20881 caccatgcaa aaattttaat cagcaatagc agcgaagcca tcgccgtcgc agcaatcgcc
    20941 gcccgactga ccagccgtcc ataagattga ttcataaata ctcccgctat caactgacgc
    21001 tagtataacg gaagcaaatc atctgcaatg cattaagcag caggcaaatt gaggataaaa
    21061 aaacccccac atcatgtggg ggaagacagg gatggtgtca caaaaaagta cctaacctga
    21121 tgatataaat ggatttatgt caaccgctgt ccacatagcg tccacatcga ccataaataa
    21181 gcccctcaac tgaggggcta tttttttgat cacatccaca taatttgctg ccctgacggc
    21241 aacgggtgcg gccttacggc gtggacttct cccggcttca cgatgtatcg ctgtaccgac
    21301 tcataagtga tgaacgtggc gctgcaattc acgttctggc actggtgata acgctctttt
    21361 gtcgtgtcag tgatatagcg acttgtacgc gcatgtgcgg catgctggca taaaggacaa
    21421 tgaaacatcg cgagcacctc ttccggtttt gttgatggtg ccattttagt taatttatcc
    21481 ttataaaaca aacagataaa taaaccacgt cactcatcat cttctgtttc gtactccaca
    21541 tcagaaagcc ttacctcaag ctccaaggac gtcgtgaagc cgctattatt gagacaatgt
    21601 gtcaccttag tgatggtcca gtcctgctcg tctatgacgc gcttaaagcc tgacacttta
    21661 accggtgttt ccgtgtaaat atctgcacga ccggtagcca ggctgatgga gaactccgct
    21721 acaccccgtt gcagtttatc ccacttcgcc tgagcggcgc gcatggcctg cgcttttgtg
    21781 gcatataccg tggtcagggc aaaaacgttg tcagcttcac cggccatgta ttcaccttcg
    21841 cgcgcttccg gtacttttgg cgctttcttc tgcgtgaccg gtttcgcttt cgggtgctcc
    21901 agtgcgcgca ggtgtttttc tttctttttg cgtttcagtt ttaccttctg cttttgcggc
    21961 ttcgggtctt tggtgtgtaa ccactttgcc gttacgccgg tgtaagctcc acggtcagca
    22021 atcgcaaaat gatgacggtc gccgtcgctg cgggtgatgg taatctgcgg gattttttta
    22081 ccgctggccg tcaccccctg ccccgctttg agaaacagca gttttcccat ttttaccgac
    22141 acctcgccgc cgttgcgttc agcaagacgg gtcaggaatt tcgcatcaga ctcctgcgac
    22201 tggtcgatgt gtggaatttt tattccggcc agtgacggag cgacactggc ttccagcctg
    22261 ttacgggagg ctatcgcctc aacaatcgca ccgagcgtaa tgtcatgcca tgagccttca
    22321 cggcgggaat tgagcgtccc gcgaaaatct gcactccggg cgcggatggt gaccacatcc
    22381 ggcgcgcccc ggtgttcaac ctcatcaacg gtaaatttcc ctttgcatac cagggcaaaa
    22441 cctttccagc cgatatacac cgtcaggaca gcgccacgaa ccggcagccc gacctgcccg
    22501 tcggcatcgt tcagttcaat atcaagctgg tcagcctcaa agccccggtt atccgtcagg
    22561 gtcatgctca tcagacggtc gctgatattg ccggtaatat ccctgctgtc gagcatcagc
    22621 atgtaatccg gcgtcagcgt actgcctgca tcaaatgtca gcgcatccag cattatcccg
    22681 cccccgtcat acccgtgaat ttagtcgcca tactgccagc cttaccgatg agcgattccg
    22741 cctgtttacc gatatcgcca taaagcgcgg ccagtgactc atcaacgcgg gtgagcgaca
    22801 gcgtaaaatc aattttccgt ggtgtgccgt ctgcaaagaa aatactccct gtttcactca
    22861 ccttgctgat gacatacatg ccgtaaatca tgccggtgcc atccagcaac ggccacgccc
    22921 ggccttcctc tgccatcagt ctgagcgtgg tcatcgtcag ctttccgcca gtcagttcgg
    22981 gataaagcac accggcaagt gtgatgttct cctcacctac accgagaaac tgataggcat
    23041 cccgtttacc gatacgggaa tttgacggcc agcgataatc tgattcacgc tgcatggtct
    23101 ggtgtggcag cgtctggcgc ataaaaacaa acatacctaa cgcgagcatc atttttcgtc
    23161 acctccttaa ccgtcatgca tcatgctggc acgggcgcgc gcacgtttat cccgctcgta
    23221 tttttcgagc gcatcctgta actggcggtc aagctgtgtc cccggtgcag taccacccgt
    23281 caggctgatg tgatattcgt ttttactctg gtccacataa gagcggccag ccggtgccgt
    23341 gaccggctga taagcctgat agcctgcata agagctggtc gccggaatat aaccaccggt
    23401 gccatacgtg gcggcatgag ttctggcggc ggtctggtca agtgtgtctg actctttgtt
    23461 gatgaccccg agcttttcca gtacccagtc aatgccgctg cgcaatttgt tgaacgcatt
    23521 aagcggcagc atcagcgcgt cagccagtgc ctgcccgaac atgacgcccg tgtcacggca
    23581 acggttcagg gtgtcctggg tggctttgac cggggcaatc aggtttttaa accactgcca
    23641 cgcagcctgt aacttttcgc ccagccagtc aaacaccggt ttcagtggcg tgaacagctc
    23701 ccccaccggc gcaaatgccg ctttcagccc ttcaaccaca ccgccaaaga atgcgctgac
    23761 aggctcccag tatttacgga taagcaacgc cccggcgaca atggcggcca ccacagccac
    23821 aaccggccag ctaatcgccc cgatggccgt cataacggca ctaccaaccg tcgtgaagat
    23881 tgcccccatt gcgcctgctg ccgcgatgat ggcattgatg ccggtgataa ccggccaggc
    23941 tacaagacca attgcaccga tgatgccagt cagcgccagc gcgccaccgg caatgaggcc
    24001 aatggttgac gccagtgatt tgtttttctg tacccagccg tcgagtttta acacatactt
    24061 tgtggccgtc tgcgtgagct tacgtagcgc gccttcctgc tggtcaaaca ggtctgtccc
    24121 caccgcctca taagcggact gaaactcctt aaagtcaccg ccgaggttgt cctgcatgat
    24181 atttaccagc tctgcggtct tcccgtctga ggctttaaac gcagcggtca gtttgtccag
    24241 ctttccggtt gacgcggcag tcatcagcac agcggcggct gagctggcct cctccccgaa
    24301 aatggttttc atgtattcgg cctgctgggc agtaccgagc cggtttttct caaagctggc
    24361 ctgcatttct ttcagaatgg taaatactgg tcgggtgttt cccttactgt ctgaggtttt
    24421 cacgccaagc tctttgagtg catcccatgc ttttccagtc ggtgcctgca ggcgacttaa
    24481 cacggcacgg cttcccgtcc ccgccattga gcctgtgatt tttgcatcat gcagcgcccc
    24541 gaccattgcg gcggtttctt caatgctgac accggcattt tttgccactg gtgcggcata
    24601 ggtcagcgca tcgctcatgc cgtcaaaatc agcggcggtt ttgttcatcg tcatggagag
    24661 aacatccccg atatgagcga ccttatcgtt tgaaagctga aaggcggatt tcatccccat
    24721 cagcagggcg gcgttttctt ccattgtgcg gcggttcgcc agcgccatat tcagcgtgac
    24781 cggcgttgcc gcctgaatgg catcaacatc cccacccgct ttcgcaatga taatctgtgc
    24841 accggccgca tcatccgctg aggcggcggt gttgtcaccg agctggcgcg cctgcttgcg
    24901 gagtgcagcc atttcggcgg agtcttttgc cacaccgagc acagcctgca attctgagtt
    24961 tttctgcgca aactcataac ctggcatcag cagcttaact ccggctatcg ttcccgcagc
    25021 agcaatcccc acaccggcag cgcccactga ggccatattt ccggccagtt cctttccggc
    25081 ctgataacgc tgtttgactg cgttaagttt tgcctgttgc gcactgacac gcgccagcgc
    25141 gtcacgctgc cggttaagct gggcggtggt ttcactgata cggtttttca gtccctgctc
    25201 atcatgtgca agattgcggg tattaattcc cacagcggcc agttcccgct gctggcgttt
    25261 aacggaatct gtcaggcggt tatatttcgc ctgtaagtcc tccgccgcac gctttgcgga
    25321 ttccagcact ttcgcctgag cacgggtcgg gcgttcggtg tttttaaact gtgtggcaag
    25381 ggcttcggcc tcctgccgtg ccttttcaag tgcatgacca gtcacggcga gctgtgcact
    25441 ggtcttgcgg aatccctcaa tacgggatgc gtgaccgttc agctcgcgca gtgatttttg
    25501 tgtttcccgg atatcccccg acagcaactt actcgctctg cggatggatt taaacgggcg
    25561 ggacgcctgg tcaacagccc tgagcaatac ctgtaatttt acattgttac tcattcgtgt
    25621 ttccgcttcg cctgagcgcc ttttcgcgcc atgtgatgag ttcggtcagg ctcatgggat
    25681 acagttctga tggcggccag tgaaatatca ctgccacatc cgccatcagg tcatcgaccg
    25741 acagattttt cggaagcgtc actgcaccga gttcggcgac aaaaaaccga ccaccttacc
    25801 ggccagcgcc acaaggtcag gcagttccag cgcggcgact tcctgctcgg tcagcatcgg
    25861 tgccgtcatg cgcggcagca ccttaatcag tgcatcgact tctgagtttg cgaccgcagc
    25921 cagactgaca ccgcgcagcg tccccgcact gggtttcatc agcgtgacct gttcgataac
    25981 ctgctcacca cgcttgaccg gattgtccag ggtaatcaca ttttctttgt tcatggtttt
    26041 ctcacttctg aatcggggtt aaccggtcag ccaggctgac cggatgaaaa ttacaggccg
    26101 atattgcggc ggtgttgctc cagccggtcg acgccgttca ccttctcaat catgttgatg
    26161 gtgtcgattt cgaccagctc cttaccgtcc atcgtcagcc ggaaataggt gcagaccacg
    26221 gagattttcg actcggtgtc ttctccctgt ttaccctcgc cggtgtcgat ttctttctga
    26281 cggccacgca tgaccacctc tacggccacc gtttcgccgg tatcgtcacg ctggtaagag
    26341 cctgcaaaac gaatcggcac ggcatccaca ccggttgcgg cgtaaagctc ccagataacc
    26401 gaatccggga agcccccgag cgaccactcc attgacagcg catcgtcatc aaggccgagg
    26461 tctaccggtg cgcttccgtt catccccgca ccgcgatagt tttcgagttt acgggtcagt
    26521 tttggcagcg tgacggactt tgcaacgccc tgatagctgt agccgttcag aaagacgttc
    26581 attaacttga gtttgcgcgg cattgccatc ggtcaggctc cttaattgct gttaaccgag
    26641 gtgaccagat ttgccaggta tttatcggta atacgctggc gcagggtcag gttttcaaga
    26701 ggaggcaccg gggtatagtc gtagtcgata tacagttttc cggccttgag ggtttccgca
    26761 tcgttggatt cttcgctgaa ccagcaggtc gcatccacga tatagccgtt tgttttcagc
    26821 tcacggaatt tggcattgat gccgtcaacg atgtcgcgaa tcagcgttgc ggtgatgggc
    26881 ttgtccaccg cccacatgtg cgcctcagcc atcgtgtcgg ccagcacctg cgcggtgcgg
    26941 gtgtagtttt caaagaggaa cagcgggtca tcagagcagg tacggttacc ccagaagcgg
    27001 aaaccgtcac ggcgaatcag cgttgtgacg cctgactcgt taagcaggtc agcatcggtg
    27061 ccggactcct gcaaatccca gaatacggac gcgctgatgc cggtaacacc gttcaccccg
    27121 acgttggaca gcgttttatg ccagccctgc tcctggtcga ttttagcgcg cagacccagc
    27181 gcacgggcgg tggcatacgc ggtggcggtg gtactggcga ccgtatccca tgcgaggaaa
    27241 tccggccaga tgaccatcag ctcacgctgg ctgaaattct gacggtaggc tttcacctcg
    27301 gaaacggtct tacagcccca tgcgctgata tacccgaaag cgcgcagctt ctgacagact
    27361 gatgccagtg caacagccac ctctttggtg tccagtcccg gcacgccgag aatacgcggt
    27421 ttaacaccgg ttaccgactc cgccgccagc agggctttca gtccggtgta ctgaccgttt
    27481 tcgtcggtgg tgccgatgat attggaaacg gtctgcgcga gtttcgtttc ctcgtcgtcg
    27541 ccggtgccgt cttccacacg cacgacaacg gtgaccggtt ttgactggtc ggcgatggcc
    27601 tgcaacgatg ccgccagcgt gcctttttta ccggcctttg caattgcgct ctgcacattg
    27661 gtaatcagca ccggtttatt gagggggaag gtttccgcat ccgcatcgct ggccgtgcag
    27721 accatgccga caatggcagt ggatacggtg gaaatgacgc gggtgccgtc gttaatctcc
    27781 agcacctgca cgccgtgatg atagtcactc atccgtttaa ctccgtggtt aatgggtgca
    27841 actattttct gttgggcagt gcatgagacg ctatttgacc tggctggtca gtggatgaaa
    27901 caacagataa agaaaatgcg ggcaattcgc ccgcatgtcc tgatttgtac acactcattt
    27961 tccgactgac agtttacata gcccaaacgc tatcaaatct gacagcctgc tatgagcgaa
    28021 aagcgttaaa tctcacgata tctgctaccg ggaaaaattt cgattatctt cgatgggaac
    28081 tttgataatg tagtaaatgc cattttcagc gatcactttt caagcgtgtg taaatgatta
    28141 atgttaaaaa attgacttca ctgaaataag gttgctagta tttttggagc aaccattaat
    28201 gttttttatt taaggaatgt aaatgacata cgcactgatt gataattcaa ctctaactgc
    28261 agtccaaaga atcgagggga aagtaaaaac aagatcgaag gactcagtag atacagacat
    28321 aatagcattt gagaactata tacagtctat tttgttctat gatcacgtta tcgcggttga
    28381 tgattatatt ccagagtacc gtgggagtag gatttcaagt ttcagagaga tattatttct
    28441 tgataaagca caatatgggt tggatgaaat tgaaagtgaa gcaaaaaaaa tatctgatgc
    28501 attgaaacct aaaattcgtg gtggaacatt tgttaatgac gattttaaaa aactaattga
    28561 acttcttcag acacatatca tatgtacatg ggacattagt tcaagtgttt atcatctaac
    28621 actgaagaac ttatctgatg gaggtaagga gtttgaaaaa tatggtaaca ttgctgctgc
    28681 cattttttca gaactgcgtg atgctgctga gtctggtaat agaacaaatg gcgatgttga
    28741 acttctcgat cgatttggca accctattcg caaggggtac aaagttcccg gagcaaaatg
    28801 gggggatggc acatctgctg gtgaagcatc agatgcaatc aaagcattcg tggcttcgct
    28861 tgtatggctg gctaatcggg cagtatttta ttctctcaca gctaagtacc ttcaagcaga
    28921 tactttttta tatccattac gtcaggcgta tcaacagaca tatttaagcc aaaactgtca
    28981 gtatggtttt gactatgcta aacatattgt agaaaacttt tccacttcgt taagtcatga
    29041 tctcattgat attcattctg gtggtaaagc tgtagcaaca gcaacttcgc ttcctttatt
    29101 ttcggcatgg ctcgcaaaag aaactggtga tccaagttgt attgttgaag ctgcttataa
    29161 cataagggac aacaacgaat ttgtaagggc ccgtgagaaa atgcgagaaa taaagcgctt
    29221 gtttgatgaa aatgaattgc ctcaagctaa taagtcagta agagcattgc ttactgaagt
    29281 atcgaaagaa tcagattata ttcgaactaa gtatgggctc aagactagac aaggtgtacc
    29341 tgtaactaag cttgtacatg tatacaatac tgttgcggct attaattccc ttccgaaact
    29401 accgaccttt aattttaaag ttaaagttcc tgatttctta tatgatctta aaaaacccaa
    29461 ggggtttcgt gctctctatc gaaatttgac taatgatctt tctactgtgt ggtcgttagg
    29521 agaagctcga gatattcttg gaagtaaagt tgtgatagat aatcatgcaa gaatttacag
    29581 tccgaagcat gaagaaccgc gtttcaaaaa ctctcattcc ccatataaga gcccgatgta
    29641 gcttaagaaa aagacacgcc caccttgtgc gtctacttct ggcacagagc ggactctcag
    29701 attaggcttt actctgtgcc atagatatgt aagctcacac cagagctcat acaacttatt
    29761 gcggcatttc cggccattca ggatttgcag gatccacacg actgaccaga acactgtagc
    29821 gttcccatgc ctccagtcga ctgcgctcct catctgttgc catattcagc ctgacagcgc
    29881 gctccagcgg caaaatcacg gattcagcat ctgcaagaag tctggctttc cgattttctg
    29941 cctgctgctg caattcctct gccgtataaa ttcgtttaat cactgtacag tccttaaaca
    30001 tccagttccc tgaaatgtcc gcccgtcggt tagcagtaat atccggcact tcaacaacac
    30061 ttaatccatc cggtctgata gctgtcacat ccttttccac atagcggata atattatctt
    30121 tgtcgtacgc tatttttatc gtgtcatcag caaaatactt ttgttcttcg taccagttct
    30181 taccatcttc tgaaaaaaac cagacaacat caaagtcctt tgtcaattga tattgttcaa
    30241 ccgtttttgg attacctgcc gttatattta tcaaatgctg cataaattat acctgtgcca
    30301 cgttatacca agtcccgtta atgtatttct gcaccggtct gtaatatacg ccaccaatgt
    30361 tatcggcaga gtttgagccg gtatcctgaa caataatgcc ggaatataca cacccggacg
    30421 gtgcctgatg tgtccatgtc ataccattgt tcgcaggttt gtatgtggca gcaccaccaa
    30481 gccggatatc ccggacatag cgtgaatcaa agttgccata gttagatggt gatacctgcc
    30541 cgttaacagc aaaagtgatg ctgttatctg tatttctctg actgtaaaaa tgccagcctg
    30601 catcatcacc taattcagcc accacaggac gacttgagtt tccccacaaa ttgaatgcgg
    30661 cttccttcgt ggatgtattg ctgctgctga ccgtgaactt tttcccgcta ccggcacgta
    30721 ctttggtact tgagacaata tcacctgtaa cactcaggcc atgccccatt gacactccgc
    30781 cattggcgtt attgatagtc agcggtctta agccgttcca tgtcccaaat ttatcgccag
    30841 aggccgtcag cattaaatat gtgctgccgc catcattcct gataaagaat ccataattgc
    30901 cataagcaat gcgcagacca ttagcactga gtgatgtaat ttcacctctt gaacggagac
    30961 cataagcgga gctgagtgat aattcttcct gagcgtcatt gtttcctgtc gcccagcgaa
    31021 ttaccccgcc ctgaactgtt tcatgccaga tagtatctcc ttctccacca cgaaactttc
    31081 tgagatattt tttgccgcct ctggtgcctg aacataaggc cgtagacata taggcattct
    31141 ggcttccgcc atcctgatta atcgttccgg tcattgagtc gccctgacga ttccagtcac
    31201 gacgccagcc gggggagtag ccgtccccat gatttatgta agtgaattgc gcgctggttg
    31261 taccgccacc gcttgatgtt gttggcgtgg tcactcggat agtgattgca gatttagttc
    31321 ccatgacctc gacgacacaa ccagccaggt ggatatcacc acatccggta tccgtaatga
    31381 ttttgttatt tgcatatgac caggagcctt tgcacatcca gtacggatga ttaaatgcac
    31441 cacgggaatc cagccattca ataaactgag cggttgtcca gtttccggct tcagtgctca
    31501 aagcgccgct ataagcacga caggcaccga tatttttcgt gaaggtatcc tttcccggaa
    31561 tatccgcacc gttctgatct ttctgaagac gtttttcagc attgtcatag gcagacttca
    31621 ctgcttttgg cgttgcggcc agcgtttcag aatcgctgtt gatggcacta cttagctgga
    31681 caagaccttt tcgcgctgtg gtggcatcct gtgcagtgta tttcccgtta gcaaggtcat
    31741 acgctgtctt aaccgccttt ggtgttgccg caagcgtttc agaatcgctg ttggtggcgc
    31801 tactgagctg gacaagacct tttcgcgctg tagtggcgtc ctgtgcagtg tatttcccgt
    31861 tagcaaggtc atacgctgtc ttaaccgcct ttggcgttgc cgcaagcgtt tcagacgtgc
    31921 tgttggtggc actactgagc tggacaagac cttttcgagc tgtggtagcg tcctgtgcgg
    31981 tatatttccc gttagcaagg tcataggcgg cctttaccgc tttcggcgtt gcggccagtg
    32041 tttcagacgt gctgttggtc gcactgctta actgagtaaa accttttgcg gtcagcgagg
    32101 cgtccgggtg acgtcgtgac tgttcgtgct ctgcaatttt gtcatcaacg taatcctgtg
    32161 tcgccatcac cgttgtggtg tcaatggtca gctccaccga ggccacactg ctgacgatga
    32221 tgaccatgcg acaggtctgc gaacgccctg agccttcggc aagagctggc ttataacttt
    32281 cggccatgtt cgccacggca attaacgttc ccgcatcatc gtacaggcca agctcacgca
    32341 tccagaaacc gcccacctcc ggcggaataa ccagctctgc gataatataa ttactgtttc
    32401 gtttgtcctg gctgattttg ttcagcgtat gtcgccagac ttcgtggata agcccggtct
    32461 gtccggcatc cgggacaggc aatttaccac cgccatcccc gacggccatc gtggtaatgt
    32521 tgaccttccg ccctcccggt gcggttgccg ctgccagctt tgctgcaccg gcagtggtga
    32581 taacggttct gaattttgtg ctcattattc ctcacttatc cggggtaaac cgtaattaca
    32641 tcgccgtcgt aagccacacc accggcgaac aggtagccgg gaatgtcccg ggtaatgttc
    32701 aggccaataa gatgacggct tgcaggtttg gcatcagcaa taagccgttc catttcctga
    32761 tacattgcct ctgtgatgcc gctttccagt acaccaatat caagccggaa ggtgccgggc
    32821 gggtcactgt tttcccacca ctccgtcacg ttgatgagat agccgagcgg ctccaccaca
    32881 cgccggattg cgccgatagt gcctttatga cagtggatga aataggcatc gcggataacg
    32941 gcgcgttttg tcgcttccgg ccacttttca tcccacctgt cgaccgaaaa cgcccacgcc
    33001 agccacggca gcagatttgc cggacaggta tccgggttcc acagttcacg aatcctgacc
    33061 ggcgtttttt caatttccgc acaggctttt gcggcggcaa cttcaagcgg tgatgagccg
    33121 gtcggcagca gtcgcgaatc actcatccga gcctccggtc acgacgcggt attcggtaca
    33181 gaaagacgcc tgcgtactgt tgagcacgat gtcggccagc ggtgcagtca gttcgacacg
    33241 ctgcacgcct tccacatgca aagcggcata aatggcagac agacggatgt cgcgccccag
    33301 ccggtgctgt gccgtgatgt acgcttccag ttttttcacg gcagcagcgc gtatgggttc
    33361 gctttcggga ccagggtaaa ggtaaagcgt ggcgtttatc tggtattcaa cgatggcggc
    33421 agactgcacg gtcacgcggt cggccaccgg cctgacgtcc tcaccattaa gggcgttacg
    33481 caccaccgcc agcaggtctt cggatgcgac accgttattt tcacgtgaca gcacagagat
    33541 ggtgacgcag gccggagacg gactggttac agaaatatcc gcgacacgcc cgtcggcact
    33601 gcgaccatga tactgatagg cccccaccga cccggcgacg cttaaacctt caaacgcctg
    33661 ctgaatacgc agacgataat ccgtgtcaga ttccatcact gccggtgtcg gcgggagggt
    33721 cgaatcatct gccggggtga taatcaggcg cgtggtgttg taattggcac caatcacatc
    33781 aaggtcatta ccggcggcac aggccagcat taccgcccgt gcggcctcat tcacacgctg
    33841 acgccagata agctcacgat aagcattttc ctccagcagt ttgacgagag gctcggattc
    33901 cagcgtcagg gtacgggcga ccgcctcctg ctggtcttcc gggtaaaggg aaatcagtgt
    33961 cgccttgcgt tcggcaagaa tggtttcaaa gtccagctcc tcgaccacat ccggtgcggg
    34021 tagctggttc aggtcgataa tcggcatggt ttcaactcac agggatggtt aacgaaagtg
    34081 gctggccggt gtcgttgtgc tggccggtta acgtgaccgt cattcgcccg tcaaagctgc
    34141 gcgccgtggt gacggatgac agggtgacgc gggcttccca tttcagcacg gccatgtaac
    34201 aggcgacctt aatctgcaac tcaagcgccg gggtctgcgg ctggtcaatc attgacgcca
    34261 gcaacgagcc gtaatcacga cgcatcaccc gtgagccgac cggcgtgcgc aggatatcgc
    34321 caatactctg gctgatatgc tcaaggtcag agacagtcag gccatcactg cgattcattc
    34381 cgagataacg cgctgtcata gaggactccc ggttgtgccg ccgctgtcgc cggggtgttt
    34441 atgggtatgc agtaccttcc cgtttgatga gagttcaccg ccggtgtgtt caatgttgcc
    34501 gcgcatcgtc ccgcccttct gcacttccag cgtgccagtg atcagcttgt ttgtgcagac
    34561 cacctccggc gtgtccagag tgacgcgggt tgatgctttc accgtgacca ccggcaccgt
    34621 ggcagtaaca gaatcagaag ccgtcacgct ggccgtttta attccgctta ccgtgagtgc
    34681 actggtttca ggttcatact caatcaccgc cccgtcaggg aaacggatat gcagggcatc
    34741 cgccgacgca gacggcgcgg ggttatcacc ggaataaatc cccggcagaa cgaacgccgt
    34801 gtcgagttca ccgcctacgg ccagaatcag cacctgttcc cccacggaag gtgcccacca
    34861 tgtgcgcgaa cgtccggcgc gatgggtcag ccactgaagc cagtcggtgc acatgccgcc
    34921 ggtctgcaca cggcagcgac cggcattaag gtcggtttcg acgataatgc cggtgcggat
    34981 catgttgcgc agtgcgcgcg cgagttcctg aatatttgcg agagtgttca taacgggaag
    35041 gatgccgccg ggtcataccg gcggcaatgt gacgatgagg tgtcaggaat ggcacaacta
    35101 acggtcgagg tgagccagga taatctcttc aatcatctgc acatcctcac cggtaaagcc
    35161 gagcagggga cgcgccggat aatgaatttt cttaccgtct ttccgggttt cttccgacag
    35221 accgaactga tgcacactgg ctattttcgg tgacttcccg ccgtaaaact ccattgatgc
    35281 ctgttcaggg ctggcgcgga tatgcaaaaa acgactggtg ataagtttcg caaacatttt
    35341 tcgcttaacg cgaccggtct tttttctggc gctctgctgc tggcgtggtg cgtagggtgt
    35401 gccgtccggg gctttctgtg ccatcacccg acgctgctga ctctgacgca gacgtttcgc
    35461 cagttcggcg ctcagtcgcc gacgccctga cggtgacagc gactcaataa gtccggtcag
    35521 cctgtcttca aaacgcttaa actcattcat cccacttact caccagttcg ccattgatat
    35581 aaagctccat cgggcgggtg accggctccg gcggcggagg ttccgggata ttcttcacat
    35641 gcagcgcgcc gtccacctca ctgaccagcg tgcgctcggt cagcatcagg ctgatgctga
    35701 tatcaaagct gctgtcattg ttgatgtctg cataaaacgt gaagcccttt ttctggcctt
    35761 cgtcggtggt catgatgtcg ggctgatttt cccgcagcca cgccagcacc ggcacgatga
    35821 gcaggtcaaa atcaccggta aagtcggtca caatcacgtt gagcgtgtaa cgcttttcga
    35881 atgacagcga cgtcgccagt gtggaggcaa tactcccgtt atccacgaat atccgcagca
    35941 tctcggggct tgttttcagc accgtgacgg catcagtcag cgccctgcgc aggctgtcgg
    36001 gtttgagcat cgttttcgtc ctgacagtgt ttaatcattt ttacctggct ggcacagcgt
    36061 gccagcgcgt tctcaagctg ccggatatca gcacttaaat cgccgttcgt ctgcgggtca
    36121 ctgcccggca tcgggcaaag gctcactttc gggcaggcgt tgtggacaat cactggcgtc
    36181 tgcgcaggct gtgcgctggt gcaaccggcg cacagcatca ggcaggtcag caccgtacca
    36241 gcggcgaaaa tcttcgtttt cattaagtaa cctcgtgatg gttttctcgc gctgagcttc
    36301 acgcttcgcg gcgttctcca gttcctgacg cagtgccacc tgcgccagct cgtttttgtc
    36361 tgccctggtg agggcaacat gaagctggtt tttcagcatg gtgatggtcg tctgctgttc
    36421 actggcgacg ttgttcgccc tgtccagcaa ggcgcgcagg ctggcatttt tgtgtttcac
    36481 cagaaacaga ccggccaccg ccagcgataa caacacaacc agcacaatca tcagttttga
    36541 catggttccc gcccctcaag acgctgacgg caggccgtac gtatcagccg gaaaaacagc
    36601 gatgccacga gataaatcag tgcggtaaaa atccacccgg cagcgaccag cgagataaac
    36661 gtcgccacca tcaccatcag agccgctgcc cgtctgcgcc acggcaccgg ctgcaaaaac
    36721 agcgacgtga caatcttcac ggccagcgat tccggcggca gctcccgccc gtagcgttcc
    36781 agcacatact ccgcggcata cacgccgaca ccaccggcaa tcacacagat aaccgtcgcc
    36841 agaatcgccc atgcagcgac aaaactgacg gccacgctct gcgggtaaat cagggacagt
    36901 gccagcatca gcgccagcga cacgttcagc atcagtgaaa gggataattt cttcatggtg
    36961 tttactccgt ttaagccggt acgccgccag cggtgcgcca gacggtgacc agtttttcca
    37021 gtgaatgctc acgctgaccg taaccggcac ccggcaggga cgcccagata ttgcgacagc
    37081 gtgaaatggc gcgctcaatg cgtcccgccc ggatgtcatc cagtgcaccg cgttcgcgga
    37141 tcaactgaat ggcgagcctg tcctgtgaca acggactgaa atccggcagg gcaagctgtt
    37201 tgcggtagtg cggccagaac aggtaaagct gctgatagcg accggaggcc gtggattttt
    37261 caccgcgacg gttaaacacc ttcgccggtc ggccatgtgc gaacgggtgg tcactgtagt
    37321 cggtgaagat ttccggcttc ccgtccagtc cggtgactat cacgtcatag ccccggtttt
    37381 tcgtcagcgg atgattcgcc gtcccttcgg acacggccag catgtcgaga aaggcggcga
    37441 tattctgatg tgtgctaatt accggcatta cggtttcccc ctgcccttaa agcggcgctg
    37501 aatggcaatc tcaatcacct gataaccggc gatacccagc atggagccga tgccgcacac
    37561 cgcaggcagt gacaggtcag gaaactgcac cagaacaaca ccggcaacca tcgagacaaa
    37621 accaccgagc aacatgcgcc cgataaacag acgcggagtg atgggttcac caccggcaag
    37681 caccttgccg acaacaatca gcaccccaat catgaaaagc gacaggacgc ttttttcttc
    37741 tgctgtcatg cgttactccc acagattgac agtttcagcc acgggcgcgg tctgaacgtc
    37801 gggcagttcg acggcggtgc cgtgtggcag caccgccccc agttcagcca gtcccggatt
    37861 tgcggcgagc acggcctcga ccacgccctc agtgcgcccg taataccgaa cacaaatggc
    37921 gtcgagcgtg tcgccctgta gcgcaaaggt cttcatcaga tttgactcac gatgcagcgc
    37981 ggcttgtcct ggatacgcgc caccgcccag cgcatatccc gccacagctc atcaatggtg
    38041 ctgtcaatgc tgtcggcctt cttgtcgccc ttggcactgg catccacgcc gcggtaacgc
    38101 tcataaagcg acgcggtcgc catcgcacac acagcgcgct cgtagtaaaa aactttgatg
    38161 ctttcaccgt cgatgtcgtc cgccgggacg tcagccagac gcgaaaaacc ggcagcaatt
    38221 ttctgttcgc ggtactcgta cagctccgca ttcgtctccg ccatgcctga cttgatggcc
    38281 tcacgcagac gggcgggggc gacggtctgc tcaaggcgca tacgttcccg gacgcgcttc
    38341 gggtcaatat cgggaaaaaa gaacgtgttt ttaatcaccg gctcgtcgcc tgccggttgc
    38401 gggataacca ccgtaccctc accggacacg ggagcctcct ttcgcggaat aatcagcgtc
    38461 atcatgacta cctctgaaaa gtcgggcggt ggacgccggt gcagtgtcag gtgattcacc
    38521 gtcactgacc ggcgtgccgc cctggcgcgg ggcgcattcg gttgttaact ggctttcttt
    38581 ttcgggcgtc cacgttttgc cggtgtcacg ctccgggtct tacgcggagt gcgggtggcc
    38641 gctttgggct gcggctccgg cttcggtttc agctcccgct ccagtcgttc aatctctttt
    38701 ttgacgcctg cctgacagtc gagctgtgtc gcacgttgca ggtgtaccag cgcacctgcg
    38761 gcatcaccag cgtcacgcag aaacagaccg gtgattttgt gcagctttgc gcgcacttca
    38821 tcaggcatgt cagccgtggc ggtcagttcg agggtgtccg tcagcaggcg gggatccaca
    38881 gactcaccgg cagcgtgagc gcgcatggcc gcaagcgcca cctcctcggt gaacatgtac
    38941 ggcggggtgc ggcggtgttt acccggcatg gtcagaccgt acttcagggc ataacgggca
    39001 atctccagcg caccggcaat atcgccggta tccagacgcc acagcatgac cgtcatcaga
    39061 atgtcatcct gtgcaccttt gccctgctcc agcacgccgt tcacccacgg caaccagaac
    39121 ggcagcagtt cgcgtttttt cgcggccttc agctcttttg aataaatcgc tttcagtgtg
    39181 cgctggtctg cggccagctt gaccagcatc tgctcataga cagttgcatg tcgcagcggg
    39241 gcggcttccc gctgcgcggt catcgctgcc gagacccgca tcatgtggcg ctgtgcggga
    39301 ctcgtcatcg gttacgctcc cggctctgcg gtcgctttag ccggtgtgga gaaatcaccg
    39361 accttaattt tttccaccag acaaccggcg gcgtagtcct ccaccacgta atcaatgttc
    39421 attgactcgt agttctccac gcggtcgagt ttcgggtttt ccacaatcac gcggcgatgg
    39481 ctgtcatcca tgtagtagat ggacaggttt tccagcttcg tgatgagcat cgcatccgcc
    39541 gggaagtacg ggacgcgtac cgccggcagg ttaccgatgc gtttctggct gatgatgacg
    39601 tcagcggcca gcatctcgct gttatcctgc tccttgttga cgatagggaa atacttgtcc
    39661 gccagtagct gacgccccac aatcaccaca aggtcagggt cttcctgata ccaaggttca
    39721 atcaggttgt tggtcgcatc catcaccagt gcatcgaggc ttacataatc accgccctta
    39781 cccacgcgga taacctcaga ggtggtgcgg ccttcctcgt cagtgacctt gctcatcacg
    39841 cgcgccgggg cttcattcgg tatttctgca gccagccgac cgccacatcc tgcagcatcg
    39901 gattactgtt gcggtcagag gtttcggcac gcttcacgcc gttaaaaccg gccatgatga
    39961 aatcaaggga ctggcgtttg ataatggcgt tacggatacg gagctggaaa tcctgataac
    40021 gcgcccacag gtcaagcgtt ttgtagcgga tataaaaatc gaagttaacc tggtcgcatt
    40081 cgtacttgtt tgacgccagc ttcgagaagt ccttcggctg acgctcggtg ccaccggcgg
    40141 tgtcggtggt gctggcgatg gagccggtga caccgatgcc aattttttcc cctttcattt
    40201 cactgaccgg cacaatgttg atgcgggtca gaaagtcaga ggactcctgc atggtgttca
    40261 tcagggtctg ggtgaccgac ggttcaacgg tgaatttttt cgacacatca ccggcgtcga
    40321 tgccgttcag ctcggcaaca cgggacaggt aagcattaaa tttaaagcgg gtttcctggc
    40381 gcatagtttt tcctgaaatt aagggttaat cgtgaaggtt ttcccggact gactgacgcc
    40441 ggtcagcagt tcgtcatcag ggcgtcaccg ccaccaccgg tggccttgct gcggcgctgc
    40501 tgggtcagac tttcggtgtg gtcgagactg tttttcaggc gggtgaatgc ctggctggtt
    40561 tcatccgccc tgtcagtcac ctcctgctta agtgcagaaa aggcggtttc catctcagcg
    40621 aggcgctgct cagtggcgct cagtttttcc tgcacatgtt cagcaacagc ggtcaccgct
    40681 tcatgcacgt cattcagacg ggcatcatcg ctggcctgtt tgcggccaaa aatggatttc
    40741 accttttcgg tcagggcggt gaacacggtt tcaggcaggt cttcaaattc cagctcaaca
    40801 ggcgttgcca ctgaaatcag gttttcaggg cttaatttga agcggttcag ggggttgtgt
    40861 tttgccgtgc ggcagaattc caggtattcc gtgccgaggc ttgccgggtc atcggtgacg
    40921 gccagaccca ccagataaca tttgccggtg ttggcaaagt tcggctgaat ttccattgag
    40981 gtgtagacct tctgcgcggc cttgttcatc gcgataaggt catcggtcgg ggtgattttc
    41041 gcaaacagcg cccatttgcc tttcagcgcc gaatcatcgt caatcttttc ggccttcagt
    41101 tcggccacat cgccataacg cttaaaaata ccgtcaggca ggatgccgcg cagatgttcc
    41161 aggttaatgc ggcaaccata gacacgcggg tcaaaggttt cggccatttc ctgaatatcc
    41221 tgcgcactga tgacacgccc gtcacaggtg tcaccctcaa cgccgatacg aaagaatttt
    41281 gagacttttt ttgccattgt caggagtcct gaatagtgat tagaggagtc acatgtcggc
    41341 atcagtttcc cgacgatgcg catcctccgc catcagtccc ggatggttta tcactgacac
    41401 aacagcacat tagcgaatcg cggggcgcga ctcagtagcc ttgccgtgta ttcatcacgg
    41461 cgaggtattc atgaccatca ccacagacac cactctttta cacgacccgc gtcgtcaggc
    41521 ggcgctgctg tactggcagg ggttttccgt gccgcagatt gccgccatgt tgcagatgaa
    41581 acgcccgacg gtgcagagct ggaaacagcg cgacggctgg gacagtgttg cccccatcag
    41641 ccgtgtcgaa atgagtctgg aagcgcggct gacccagctc atcatcaaac cgcagaaaac
    41701 cggcggtgac ttcaaggaaa ttgacctgct cggacgccag attgaacgac tggcacgggt
    41761 aaaccgctac agccagaccg gcaacgaggc agaccttaat ccgaacatcg ctaaccgcaa
    41821 caaaggcggg cgtcgcaaac cgaaaaagaa ttttttcagt gacgaggcca tcgaaaagct
    41881 ggagcagatt ttctttgagc agtctttcga ctatcagttg cactggtatc gtgccgggct
    41941 tgagcaccgc atccgcgata tcctgaaatc ccgccagatt ggcgcgacgt tttatttttc
    42001 ccgcgaggcg ctgctgcgcg ccctgaaaac cggccataac cagatttttc tgtcggccag
    42061 taaaacgcag gcgtatgtgt tccgtgaata catcatcgcc tttgctcgtc tggttgacgt
    42121 tgacctgacc ggtgacccga ttgtcctggg caataacggc gcaaaactga tttttctcgg
    42181 caccaactcc aacaccgcac agagccataa cggcgacctg tacgtcgacg agattttctg
    42241 gatcccgaat tttcaggtac tgcgtaaggt ggcatcaggt atggcctcac agagtcacct
    42301 gcgctcgacc tatttctcca ccccgtccac gctggcgcac gacgcctacc cgttctggtc
    42361 gggtgaactg ttcaaccggg gacgcgccag cgccgccgaa cgtgtggaaa tcgacgtcag
    42421 tcataacgcc cttgccggtg gtcttctctg tgcggacggc cagtggcggc agattgtcac
    42481 cattgaggac gccctgaaag gcggctgcac attgttcgac attgagcagc tcaaacgcga
    42541 aaacagcgcc gacgatttta aaaacctgtt catgtgtgaa tttgttgacg acaaggtatc
    42601 ggtgttcccg ttcgaggagc tgcaacgctg catggtcgac acgctggaag aatgggaaga
    42661 ctacgcgccg tttgccgcaa atccgttcgg ctcccgcccg gtatggattg gttacgaccc
    42721 gtcacaccgt ggcgacagcg ccggatgcgt ggtgctggca ccgccggtgg tggccggtgg
    42781 caaattcaga atacttgagc gtcaccagtg gaaaggcatg gactttgcca cccaggcgga
    42841 gtccatccgc aaactcaccg aaaaatacaa cgtcgaatac atcggtattg atgccaccgg
    42901 cctcggtgtc ggcgtgttcc agctcgtgcg ctcgttctat cccgccgcgc gcgatatccg
    42961 ctacacgccg gaaatgaaaa ccgcaatggt gctcaaggcc aaagacgtca tccgccgtgg
    43021 ctgtctggaa tatgacgtca gcgccaccga catcaccagc tcgttcatgg ctatccgcaa
    43081 gaccatgacc agcagcggac gcagtgccac ctatgaggcc agccgcagcg aggaagccag
    43141 ccacgccgac ctcgcctggg caaccatgca cgccctgtta aatgagccac tcaccgccgg
    43201 tatcagcact ccgctgacat ccaccattct ggagttttac tgatgagcaa gaaaaaaggg
    43261 aaaacaccgc gacctgcggc aaaaacaatc accgccagcg ccccgaaaat ggaagcattc
    43321 acctttggtg agccggtgcc ggtactcgac cgccgtgaca ttctggatta cgtcgagtgc
    43381 atcagtaacg gcagatggta tgagccgccg gtcagcttta ccggtctggc aaaaagcctg
    43441 cgtgctgccg tgcatcacag ctcaccgatt tacgtcaaac gtaatattct ggcttcaacg
    43501 tttatcccgc acccgtggct ttcccagcag gatttcagcc gttttgtgct ggattttctg
    43561 gtgttcggta atgcgtttct ggaaaagcgt tacagcacca ccggtaaggt catcagactg
    43621 gaaacctcac cggccaaata tacccgtcgt ggcgtggagg aggatgttta ctggtgggtg
    43681 ccgtccttca acgagccgac agccttcgcg cccggctccg tgtttcacct gctggagcct
    43741 gatattaatc aggagctgta cggcctgccg gaatatctca gcgcccttaa ctctgcatgg
    43801 ctgaatgagt cggccacgct gttccgccgc aagtattacg aaaacggcgc acatgccgga
    43861 tacatcatgt acgtcaccga tgccgtgcag gatcgcaacg atatcgaaat gcttcgcgaa
    43921 aacatggtta agtcgaaagg ccgcaataac tttaaaaatc tgtttctcta tgccccacag
    43981 gggaaagccg acggcattaa aattatcccg ctcagtgaag tggcaacgaa ggacgatttt
    44041 tttaatatca aaaaagccag cgccgctgac ctgctggacg cgcaccgcat cccctttcag
    44101 ttgatggggg gcaagccgga gaacgtcggg tcgctgggtg atattgagaa agtggcaaag
    44161 gtctttgtcc gcaatgagct tatcccgtta caggacagga tccgcgagat aaacggctgg
    44221 ctcggtcagg aggtcatccg ctttaaaaac tactcactgg acactgacaa cggctgaaca
    44281 tcgccgcctg cgggcggctt ttttacaccc cgccatcacg ccctcacacg ctcatcaccg
    44341 cacaaaaaat cccgcagaca caccaacgcc caacgggcag actaagcgcc gtcacgacgc
    44401 gctgagacgc tggaaaataa aatcagcacc accgccagcg cgcagtgctt tccccgcctc
    44461 gcccgcccgc ttcatggggc ggttttaatg cagttgcatg aacactccaa gagcacgcca
    44521 gcgctagtga taactaacaa taaaagcgac ttctgacgca tgcaaatcca tgcacatgat
    44581 ggcatttttc atgatctatg cggtatgcta tccgcttcaa tttttaacac ttgttaaggg
    44641 aactagacat ggacattatt gatttaaaat ctcaactaga aaatgaagat aatgccaaga
    44701 aaagagcaga gttgcttgct cagctagagg tgataaagca aaacgctgaa gagcaaattc
    44761 gtgctgagca aaaggatgtg gattttgaga ccaaagaatt taccgttgag ttattagtta
    44821 ataagtatca taacggatta gaggatgata ctaacgaact ctttgttcct gattatcaaa
    44881 gggattttgt ttggagtgaa aaaagacaat caaaattgat agagtctttg attttgggtt
    44941 ttcctatacc atatatattt atagcagatg ttctctccga agatcccgat ttagatgggc
    45001 gaattgagat tgttgatggc tcacaacgag ttaggactat acacgctttt atccaaaatg
    45061 aattagtttt acaagacctg aaatctttag acgtccttaa tggtttcaaa ttccaagatt
    45121 taccattatc acgtcaacgt cgtttcatga gaattcctgt ccgcgttata gaattgagtt
    45181 ctaaatgcaa tgaagaaacc cgtagagatc tttttgagcg tattaactct ggcagcgata
    45241 tattgaaaga tatggaagtc cgtaaaggat cagaacttgg ttcaacaaat ttatatacac
    45301 aggttattaa gccatgctca acaataccta aatttaaaga attagcacct ttatctcaga
    45361 ctaaagaaaa acgagatgaa cggttagaat ttacattaag attctttgcc tacgtagata
    45421 agtacaagaa ctttgatcat tccgtacgag attttctaaa tgactatatg tcagaaaatc
    45481 gagatataga cgaaactaaa caaaactcaa tgaaacaaga gtttgctaat gttctatctt
    45541 ttgttgaaaa ctatttccca gcaggattca agaaaactat tactgcaaaa tctacacctc
    45601 gagttagatt cgaatctctc gctgttggcg cagcacttgc attacgtgaa gtccctgacc
    45661 ttgttcccaa gaacttagat tggctaagct cagaagaatt caaacgattg accacttccg
    45721 acggagctaa tagtcgcgtg aaggtaattg aacgaattga atatgttcgg gataaattat
    45781 tagaggatta acatggatcg ttttcgtgag gattttgacg aaaggtcagg agagatcttg
    45841 gcctatttgg atcttttgaa atttattgag tatgccggtg cagaactgat atcagctgat
    45901 gataaagaac ataagttctc cattactgct caatcacgaa aaactctaaa gggagcagta
    45961 tatatactgt tatataacct cattgaatca acaatgagag aagctatctg cc
//
LOCUS       DAZOZY010000009        44375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZOZY010000009 DAZOZY010000000
VERSION     DAZOZY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.009"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            111..854
                     /locus_tag="ACHURQ_000462"
     CDS             111..854
                     /locus_tag="ACHURQ_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0822335.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(911..2344)
                     /gene="bglA"
                     /locus_tag="ACHURQ_000463"
     CDS             complement(911..2344)
                     /gene="bglA"
                     /locus_tag="ACHURQ_000463"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR0822336.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            2389..2700
                     /gene="yqfB"
                     /locus_tag="ACHURQ_000464"
     CDS             2389..2700
                     /gene="yqfB"
                     /locus_tag="ACHURQ_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR0822337.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            2864..3523
                     /locus_tag="ACHURQ_000465"
     CDS             2864..3523
                     /locus_tag="ACHURQ_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR0822338.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(3719..4699)
                     /gene="ygfZ"
                     /locus_tag="ACHURQ_000466"
     CDS             complement(3719..4699)
                     /gene="ygfZ"
                     /locus_tag="ACHURQ_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR0822339.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            4942..5208
                     /gene="sdhE"
                     /locus_tag="ACHURQ_000467"
     CDS             4942..5208
                     /gene="sdhE"
                     /locus_tag="ACHURQ_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR0822340.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            5189..5596
                     /locus_tag="ACHURQ_000468"
     CDS             5189..5596
                     /locus_tag="ACHURQ_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR0822341.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(5636..6157)
                     /gene="fldB"
                     /locus_tag="ACHURQ_000469"
     CDS             complement(5636..6157)
                     /gene="fldB"
                     /locus_tag="ACHURQ_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR0822342.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            6269..7165
                     /gene="xerD"
                     /locus_tag="ACHURQ_000470"
     CDS             6269..7165
                     /gene="xerD"
                     /locus_tag="ACHURQ_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR0822343.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            7190..7900
                     /gene="dsbC"
                     /locus_tag="ACHURQ_000471"
     CDS             7190..7900
                     /gene="dsbC"
                     /locus_tag="ACHURQ_000471"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR0822344.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            7906..9639
                     /gene="recJ"
                     /locus_tag="ACHURQ_000472"
     CDS             7906..9639
                     /gene="recJ"
                     /locus_tag="ACHURQ_000472"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR0822345.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            9730..10828
                     /gene="prfB"
                     /locus_tag="ACHURQ_000473"
     CDS             join(9730..9804,9806..10828)
                     /gene="prfB"
                     /locus_tag="ACHURQ_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR0822346.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            10838..12355
                     /gene="lysS"
                     /locus_tag="ACHURQ_000474"
     CDS             10838..12355
                     /gene="lysS"
                     /locus_tag="ACHURQ_000474"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0822347.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(12398..12946)
                     /gene="idi"
                     /locus_tag="ACHURQ_000475"
     CDS             complement(12398..12946)
                     /gene="idi"
                     /locus_tag="ACHURQ_000475"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR0822348.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            13001..13072
                     /locus_tag="ACHURQ_000476"
     CDS             13001..13072
                     /locus_tag="ACHURQ_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822349.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(13069..13194)
                     /gene="yqfG"
                     /locus_tag="ACHURQ_000477"
     CDS             complement(13069..13194)
                     /gene="yqfG"
                     /locus_tag="ACHURQ_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR0822350.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(13196..14274)
                     /locus_tag="ACHURQ_000478"
                     /pseudo
     CDS             complement(13196..14274)
                     /locus_tag="ACHURQ_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            14710..16629
                     /gene="ygfT"
                     /locus_tag="ACHURQ_000479"
     CDS             14710..16629
                     /gene="ygfT"
                     /locus_tag="ACHURQ_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR0822351.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            16629..17117
                     /locus_tag="ACHURQ_000480"
     CDS             16629..17117
                     /locus_tag="ACHURQ_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0822352.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(17186..17536)
                     /gene="ghxQ"
                     /locus_tag="ACHURQ_000481"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(17186..17536)
                     /gene="ghxQ"
                     /locus_tag="ACHURQ_000481"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(17572..17886)
                     /locus_tag="ACHURQ_000482"
     CDS             complement(17572..17886)
                     /locus_tag="ACHURQ_000482"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0822353.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(17819..20758)
                     /locus_tag="ACHURQ_000483"
     CDS             complement(17819..20758)
                     /locus_tag="ACHURQ_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR0822354.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(20755..21534)
                     /gene="ygfM"
                     /locus_tag="ACHURQ_000484"
     CDS             complement(20755..21534)
                     /gene="ygfM"
                     /locus_tag="ACHURQ_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR0822355.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(21585..22913)
                     /gene="ssnA"
                     /locus_tag="ACHURQ_000485"
     CDS             complement(21585..22913)
                     /gene="ssnA"
                     /locus_tag="ACHURQ_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR0822356.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(22916..26014)
                     /gene="ygfK"
                     /locus_tag="ACHURQ_000486"
     CDS             complement(22916..26014)
                     /gene="ygfK"
                     /locus_tag="ACHURQ_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR0822357.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(26336..26914)
                     /gene="mocA"
                     /locus_tag="ACHURQ_000487"
     CDS             complement(26336..26914)
                     /gene="mocA"
                     /locus_tag="ACHURQ_000487"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR0822358.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            27017..27787
                     /gene="yqeC"
                     /locus_tag="ACHURQ_000488"
     CDS             27017..27787
                     /gene="yqeC"
                     /locus_tag="ACHURQ_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR0822359.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            27835..29460
                     /gene="yqeB"
                     /locus_tag="ACHURQ_000489"
     CDS             27835..29460
                     /gene="yqeB"
                     /locus_tag="ACHURQ_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR0822360.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(29501..30433)
                     /gene="arcC"
                     /locus_tag="ACHURQ_000490"
     CDS             complement(29501..30433)
                     /gene="arcC"
                     /locus_tag="ACHURQ_000490"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0822361.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(30481..31866)
                     /gene="hyuA"
                     /locus_tag="ACHURQ_000491"
     CDS             complement(30481..31866)
                     /gene="hyuA"
                     /locus_tag="ACHURQ_000491"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR0822362.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(31919..33130)
                     /locus_tag="ACHURQ_000492"
     CDS             complement(31919..33130)
                     /locus_tag="ACHURQ_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR0822363.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(33188..34384)
                     /gene="dpaL"
                     /locus_tag="ACHURQ_000493"
     CDS             complement(33188..34384)
                     /gene="dpaL"
                     /locus_tag="ACHURQ_000493"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR0822364.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(34442..35629)
                     /gene="ygeW"
                     /locus_tag="ACHURQ_000494"
     CDS             complement(34442..35629)
                     /gene="ygeW"
                     /locus_tag="ACHURQ_000494"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR0822365.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            36108..37886
                     /locus_tag="ACHURQ_000495"
     CDS             36108..37886
                     /locus_tag="ACHURQ_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR0822366.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(37926..38405)
                     /gene="xdhC"
                     /locus_tag="ACHURQ_000496"
     CDS             complement(37926..38405)
                     /gene="xdhC"
                     /locus_tag="ACHURQ_000496"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR0822367.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(38402..39280)
                     /gene="xdhB"
                     /locus_tag="ACHURQ_000497"
     CDS             complement(38402..39280)
                     /gene="xdhB"
                     /locus_tag="ACHURQ_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR0822368.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(39291..41588)
                     /gene="xdhA"
                     /locus_tag="ACHURQ_000498"
     CDS             complement(39291..41588)
                     /gene="xdhA"
                     /locus_tag="ACHURQ_000498"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR0822369.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            42003..42758
                     /gene="actS"
                     /locus_tag="ACHURQ_000499"
     CDS             42003..42758
                     /gene="actS"
                     /locus_tag="ACHURQ_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR0822370.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            42837..42906
                     /locus_tag="ACHURQ_000500"
     tRNA            42837..42906
                     /locus_tag="ACHURQ_000500"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42869..42871,aa:Gly,seq:ccc)
     gene            43111..>44375
                     /locus_tag="ACHURQ_000501"
     CDS             43111..>44375
                     /locus_tag="ACHURQ_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR0822371.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        10815 a        10898 c        11616 g        11046 t
ORIGIN      
        1 gccctctccc tgaaagggcg aggggaaaag cgtgccaaca ttgaagattg agccagtttg
       61 ttagcaatct caaagatacg tcaacgaatt aatttttctc ggaaaaacaa atggctatag
      121 cacttgtgac cgatggcagt cgcggcatcg ggcgggcaac tgcattactg ttggcgaaag
      181 aagagtatac ggtggcggtt aattatcaac aaaatctcca tgcggcgcag gaagtggtga
      241 acttaatcac gcaagccggt ggcaaggcat tcgtgctcca ggcggatatc agcgacgaaa
      301 atcaggtcat cgcaatgttt acagcaatcg atcagcacga tgaaccgcta gcagcgctgg
      361 tcaataacgc cgggatcttg tttacccagt gcaccgttga aaaccttacc gcagagcgaa
      421 tcaaccgagt actttccacc aacatgacgg gatattttct ctgctgccgc gaggcggtaa
      481 aacgcatggc gcttaaaaat ggtggcagtg gcggcgctat cgtcaatgtc tcttcggtgg
      541 cctcacggtt gggttcgcca ggggaatatg ttgattacgc ggcatcgaaa ggggcgattg
      601 atacgttaac caccggacta tcgctggaag tcgccgcgca gggggtccgc gttaactgcg
      661 tgcggccagg gtttattttt accgaaatgc acgccagcgg cggcgagcct ggacgcgtcg
      721 atcgcgttaa gtcgaacatc cccatgcagc gtggtggaca ggcagaagag gtcgcgcagg
      781 ccattgtctg gctactaagt gataaagcct cttacgtcac gggaagtttt atcgatttgg
      841 cgggcgggaa ataaaacagg gaagttgtct gaccggatgc aacaagtatt gcatccggta
      901 cttcatcgac ttaaagcttc tcgccgttgc tggtaatcac ctctttgtac cagttaaagc
      961 tcttcttacg tgaacgcgac atatcgccag taccgtcgtc atgtttattc acatagataa
     1021 agccgtagcg tttgctgtac tgcccggtgg tgaacgatac gcagtcgatg cagccccacg
     1081 gtgtgtagcc catcagatcc acgccatcgt aagtcaccgc ttttttcatc tcttcgatat
     1141 gggcgcgcag gtagtcaatg cggtagtcgt cgttgatgct gccatcttct tccactttgt
     1201 cgtaagcgcc aaaaccgttt tcgacaataa acagcggcct ctgataacgc tcatacagtt
     1261 cgcaaagggc atagcgcaga cctactggat caatctgcca gccccagtca gacgctttaa
     1321 catacgggtt cggtacgctg ccttcgaaac cagagatcgc atcgccggtg ccgccttcgg
     1381 ccttcactgc gttggtcatg taatagctga aaccaagata atcgcaggtg ccttcgcgca
     1441 gtacatcgag atcgccgtct tccattttga tgttaaatcc gcgacgctcc cactcgttca
     1501 acacatagga cgggtaatag ccgcgtagct gcacatcggt aaagacgtag cgttcgcgca
     1561 tcgactcctg agcgaacatc acatcgtccg ggttacagca gtagggatag agcggcacca
     1621 tcgccagcat acagccgact ttcatctccg ggttaatgcg acgcgcagct ttcaccgcca
     1681 gggcgctggc gacaaactgg tgatgcagca cctgatacat cgtctcttcc gggttctcat
     1741 gctcggtata aaccacgccg gagcagcagt aaccgaacag cggtgcacgc cagttacgct
     1801 ggttgttaat ttcgttgaag gtcatccagt atttgacttt atgcttatag cgttcaaaca
     1861 ccacttccgc gaaacggaca aagaaatcaa ccactttacg gttggtccag ctaccgtatt
     1921 gctgcaccag atgcagcggc atttcaaagt gggagagggt gatcaccggt tcgatgttgt
     1981 atttcagcag ttcatcgaac atatcatcgt agaacttcag cccttcttcg tttggctgag
     2041 cttcatcgcc ttttggaaaa atgcgcgtcc aggctatgga agtccgaaaa catttgaagc
     2101 ccatttcggc aaatagcttg atgtcttcct tgtagtgacc ataaaaatca acggcttcat
     2161 ggtttggata gtatttgcct ggcaagactt ctttggtgat ttcgcgcggc acgccgtgtg
     2221 cgccaccggt cagaacgtca caaatgctcg gccctttgcc gcctttgttc cagccgcctt
     2281 cgacctgatg agcggcaact gcgccgcccc ataagaaatc tttctgtaag gtgagttttt
     2341 tcactatcat aggctccaga tgagtcaatt cttacaggaa atagtaccat gcagccaaac
     2401 gacatcactt tttttcaacg tttccaggat gacattctgg ctgggcgtaa aaccatcacc
     2461 atccgcgacg agtctgaatc gcacttcaaa acgggtgatg tgcttcgtgt cgggcgtttt
     2521 gaagatgacg gttatttttg cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat
     2581 acgctgacgg aaaaacatgc agagcaggaa aatatgaccc tgactgaact gataaaggtc
     2641 attgccgaca tctatcccgg tcagacacag ttttatgtga ttgaatttaa atgtctttaa
     2701 attgaactca cgcctgttag ttgaaatttc gcatttaatt tcatgatttt taggagatta
     2761 gtctcaaatc agaatttatt ttagctaaca ggtgttcact ggaactattc tcagttacgc
     2821 tggagaggta tacatgtgcg tgtacgtttc tccggagtaa gttatggttc agaagcccct
     2881 cattaagcag ggatattcgc tggcagagga aattgccaac agcgtcagtc acggcattgg
     2941 gttggtgttt ggtatcgttg ggctggtgtt gctactggtt caggcggtgg atctcaatgc
     3001 cagcgccaca gcgataacca gttacagcct ctatggcggc agtatgatcc tgctgttcct
     3061 cgcttcgacg ctctatcacg ccattcctca tcaacgggca aaaatgtggc tgaagaaatt
     3121 tgaccattgc gctatttacc tgttgattgc cggaacctac acgccgtttt tgctggtggg
     3181 gctggattct ccgttagcgc gcgggttgat gattgttatc tggagcctgg cattgctggg
     3241 tattctgttt aaactgacca tcgcgcaccg attcaaaatt ttatctctgg tgacctatct
     3301 ggcgatgggc tggctgtcgc tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg
     3361 cagcgttacc ttattggcgg taggcggcgt ggtttattcg ctcggagtga ttttctatgt
     3421 ctgcaaacgc attccctaca atcatgccat ctggcacggc ttcgtgctcg gcggcagtgt
     3481 gtgccacttt ctggcgatct atttgtatat tgggcaggcg taatgggtat gcctttgtag
     3541 gcctgaaaag acgcgtcagc gtcgcatcag gcatcggagc acaactgccg gattcggcgt
     3601 gaacgcctta tccgatctac gggcctcgtc cctgttgtga taaaggcatc aaatcccaga
     3661 catgataaga cgcgtcagcg tcgcatcagg cgataaatgc ctgatgcgat aaagagattt
     3721 actcttcgag cgaatacggc agcggctcaa tatgcaatgt attcgcatcg tcgcgtacgc
     3781 ggaagatgct atccggttcc atatcgttat tcatgacgac ctgtaccacg acctgaccat
     3841 cttccagttt taccgcagcc agcaccgtac cggtacgacg ccagttctcg cccattttca
     3901 gctctaagtc ttcaccagct tccggcagtc ggctggcgct acctgccagc aaccagagcg
     3961 cacgtttatt ggcaccacgg aattttgctc gcgccaccat ctcttgtccg gtataacagc
     4021 ctttcttaaa gctgataccg cccagcgcct ggagattggt cgcctgtggg ataaactgcc
     4081 cgctgttggc ggcatcaatc accgggaaac ccgcttcaat gtttaatgcc agccactgtt
     4141 ggctattgtt cagttccgct tcaccgcgca gtttatcggt cagcatatta gcagtagctt
     4201 catcggttac gatcaggaaa cgttctgccg ggtattcaaa ccatagcaaa gtggtcgcgc
     4261 cttctttaac tacctgtttt tctttcgaag gcagttcact aaagatattt gccagcgcgg
     4321 cgcgcgcctg aaaaccggca acaccaagca gcacatgctc gtcgtctggc gcgatggtca
     4381 ctttagagaa caccgcatat tttttcagtt cagtcagctg cggttcacgc acgctgcgcc
     4441 gttcaatcca cgcaaagcca tcgccgtcgc ggaacagacg taaattgctc cacattttgc
     4501 ctttggcgtc gcaatgggcg gcgagcaggt gctgatcttc tgccatctgg ctgacatctg
     4561 ctgtcacctg accctgcata tatttttcgc tgtccgcgcc agtaatggtg gcaagcgccc
     4621 agtcatcaag cgtcatcagc gtcagcggta aacgggcaga agccgtaggc tgacggggag
     4681 gaaaaggtgt aaaagccata atagagtcct gagttgctta acggcgtatt aatgactaat
     4741 ggtaaaagag ctatgaggca atgcaagtgc tttcactacg cgatctgtgc cttctgcgag
     4801 gcgacttcca aaaaaatgaa gccattgaat tgttgtgaat taatttcgtg atcgcgatgg
     4861 tgtttactga tattcccgta aaagaaggcg aaaaacacgt tacaatagcg gggtactgga
     4921 ttttcgtgag acaggaagaa gatggacatt aacaacaaag cccgcattca ttgggcatgc
     4981 cgccgtggta tgcgcgaact cgatatttca atcatgccgt ttttcgaaca tgagtacgac
     5041 agcttaagcg atgacgaaaa acgcatcttt attcgtctgc tggaatgtga cgatccggac
     5101 ctgtttaact ggctgatgaa tcacggtaaa ccggccgatg cagaactgga aatgatggtc
     5161 cgactcatcc agacacggaa ccgggaacgt ggtcctgtgg caatctgatt tgcgcgtctc
     5221 ctggcgcgca cagtggcttt ccttgctgat tcatgggctg gttgccgctg ttattttact
     5281 catgccctgg ccactcagtt acaccccgtt atggatggtg ttactttcgc tggtggtgtt
     5341 tgattgcgtt cgcagccagc ggcgtattaa tgctcgccag ggggaaattc gcttgttgat
     5401 ggacgggcgt ttgcgttggc aagggcagga gtggagcatc gtcaaagcac cgtggatgat
     5461 taagagcggc atgatgctgc gtttacgttc tgatggcggt aaacggcaac atttatggct
     5521 ggcagccgac agcatggacg aagccgaatg gcgggattta cggcggattt tgttgcaaca
     5581 agagacgcaa agataaagat gacggcaaag catatgcgct aagccgtcag tgagatcagg
     5641 cgtaatgctc tgccatttcg ttgaggattt gctcgcacca gctctgaata cgctcgtcgc
     5701 taaggtcata ctggttagtt tcatccagcg ccagacccac gaacagttgc ccgtcagcaa
     5761 tcaccggttt cgggctggta aattcatatc cttccgttgg ccagtagccg acgaacttca
     5821 cgcctttggt cgagagtttg tcatgcagca taccgagcgc atcgaggaac cactcgccgt
     5881 atcccagttg atcgccaagc ccatacagcg caacaatttt accttcaagg ttcaggtcgt
     5941 cgagctgatc ccagacggct tcccagtctt cctggatttc accaaaatcc caggtcggga
     6001 tacccagaat gagcacatcg tactgctcca ttaatttcgg ggagtcgtcc ttgaggttat
     6061 gtaaggtcac cagttctggg ccgataatat cgcggatttt ttctgccgcc atttcggtgt
     6121 aacaggtgct ggaaccgtaa aaaagaccca tattcataac gtaagcttct cgattctgct
     6181 gtggcttttg cagctagtgt atcagaatcg ctttagtcac ggcacaataa ggcataatgg
     6241 atcaaatgag tgaccataag gggcgcaagt gaaacaggat ctggcacgca tcgagcagtt
     6301 tcttgatgct ctgtggctgg aaaaaaatct ggctgaaaat acgttgaacg cttaccgtcg
     6361 cgatctgtca atgatggtgg agtggttgca tcaccgcggg ttgacgctgg cgacggcgca
     6421 aagtgacgat ttgcaggcat tactggcaga acggctggag ggcgggtata aagccaccag
     6481 ctcagcgcgt ttgctgagtg cagtgcgccg attgttccag tatctttatc gcgaaaagtt
     6541 tcgtgaagac gatcccagtg cgcatctcgc ttcaccgaaa ttgccccagc gtttgccaaa
     6601 agatttaagc gaagcgcagg tcgaacgttt attacaggcg ccattaattg atcagccact
     6661 ggagctacgc gataaagcca tgcttgaagt gttgtatgct accgggctgc gtgtctctga
     6721 actggtcggg ctgacaatga gtgatatcag cctgcgtcag ggcgtagtgc gggtcattgg
     6781 taaaggcaac aaagagcgcc tggtaccgtt aggtgaagag gcggtttact ggctggaaac
     6841 ctatctggaa catgggcgtc cgtggctgct gaatggtgtg tcaattgacg tgttgtttcc
     6901 cagccagcgt gcgcagcaga tgacgcgaca gaccttctgg caccgcatta aacattatgc
     6961 cgtgctggcg ggtatcgaca gcgaaaagct gtcaccgcat gtgttgcgtc acgcttttgc
     7021 cactcattta ttaaatcatg gtgcggattt acgcgtggtg cagatgctac tgggccacag
     7081 cgatctttcc accacgcaaa tttatacgca tgtcgctacc gagcgtctgc ggcaacttca
     7141 tcaacagcat cacccgcggg cgtgatgtct gaaaagaacg ggaagattta tgaagaaagg
     7201 ttttatgttg tttactttgt tagcggcgtt ttcaggtttt gctcaggctg atgacgcggc
     7261 aattcaacaa acgttagcca aaatgggcat caaaagcagc gatattcagc ccgcgcccgt
     7321 agctggcatg aagacagttc tgactaacag cggcgtgttg tacatcaccg atgatggtaa
     7381 acatatcatt caggggccaa tgtatgacgt tagcggcacg gctccggtca atgtcaccaa
     7441 taagatgctg ttaaagcagt tgaatgcgct tgaaaaagag atgatcgttt ataaagcgct
     7501 gcaggaaaaa cacgtcatca ccgtgtttac tgatattacc tgtggttact gccacaaact
     7561 gcatgagcaa atggcagact ataacgcgct ggggatcacc gtgcgttatc ttgctttccc
     7621 gcgccagggg ctggacagcg atgcagagaa agaaatgaaa gctatctggt gtgcgaaaga
     7681 taaaaacaaa gcgtttgatg atgtgatggc aggtaaaagc gtcgcaccag ccagctgcga
     7741 cgtggatatt gccgaccatt acgcacttgg cgtccagctt ggcgttagcg gtactccggc
     7801 agttgtgctg agcaatggca cacttgttcc gggttaccag ccgccgaaag agatgaaaga
     7861 attcctcgac gaacaccaaa aaatgaccag cggtaaataa ttcgcgtgaa acaacagata
     7921 caacttcgtc gccgtgcagt cgatgaaacg gcagacttgc ccgctgaatt gcctcccttg
     7981 ctgcgccgtt tatacgccag ccggggcgtg cgcagtgcgc aagaactgga acgcagtgtt
     8041 aaagggatgc tgccctggca gcaactgagc ggcgtcgaaa aggccgttga gatcctttac
     8101 aacgcctttc gcgaaggaac gcggattatt gtggtcggtg atttcgacgc cgacggtgcc
     8161 accagcacgg ctctaagcgt gctggcgatg cgctcgcttg gttgcagcaa tatcgactac
     8221 ctggttccaa accgtttcga agacggttac ggcttaagcc cggaagtggt cgatcaggcc
     8281 catgcccgtg gtgcgcagtt aattgtcacg gtggataacg gtatttcctc ccatgcgggg
     8341 gttgaacacg ctcgctcgtt gggcattccg gttattgtta ccgatcacca tttgccgggc
     8401 gaaacattac ccgcggcgga agcgatcatt aaccctaact tgcgcgactg taatttcccg
     8461 tcgaaatcac tggcaggcgt gggtgtggcg ttttatctga tgctggcgct gcgcaccttt
     8521 ttgcgcgatc agggctggtt tgatgagcgc ggcatcgcaa ttcctaacct ggcagaactg
     8581 ctggatctgg tagcgctggg gacagtggcg gacgtcgtac cgctggacgc taataatcgc
     8641 attctgacct ggcaggggat gagtcgcatc cgtgccggaa agtgccgtcc ggggattaaa
     8701 gcgctgcttg aagtggcaaa ccgtgatgca caaaaactcg ccgccagcga tttaggtttt
     8761 gcgctggggc cacgtctcaa tgctgccgga cgactggacg atatgtccgt cggtgtggcg
     8821 ctcttgctgt gcgacaacat cggcgaagcg cgcgtgctgg caaatgaact cgatgcgcta
     8881 aaccagacgc gaaaagagat cgaacaagga atgcaggttg aagccctgac cctgtgcgag
     8941 aaactggagc gcagccgtga cacgctaccc ggcgggctgg caatgtatca ccccgaatgg
     9001 catcaggggg ttgtcggtat tctggcttcc cgtattaaag agcgttttca ccgtccggtt
     9061 atcgcctttg cgcccgcggg tgatggtacg ctgaaaggtt ctggtcgctc cattcagggg
     9121 ctgcatatgc gtgatgcgct ggagcgatta gacacactct accctggcat gatgctgaag
     9181 tttggcggtc atgcgatggc ggcgggtttg tcgctggaag cggataaatt cgaactcttt
     9241 caacaacggt ttggcgaact ggttactgag tggctggacc cttcgctatt gcaaggcgaa
     9301 gtggtgtcag acggtccgtt aagcccggcc gaaatgacca tggaagtggc gcagctgctg
     9361 cgcgatgctg gcccgtgggg gcagatgttc ccggagccgc tgtttgacgg tcatttccgg
     9421 ctgctgcaac agcggctggt gggcgaacgt catttgaaag tcatggtcga accggtcggc
     9481 ggcggtccgc tgctggatgg tattgctttt aatgtcgata ccgccctctg gccggataac
     9541 ggcgtgcgcg aagtgcaact ggcttacaag ctcgatatca acgagtttcg cggcaaccgc
     9601 agcctgcaaa ttatcatcga caatatctgg ccaatttagc gtcatcttct ctataaaaaa
     9661 gagcgtggat tgggtacaat cccgctctta tcaccgcatt ttgactagct caataaaaga
     9721 aatcagacca tgtttgaaat taatccggta aataatcgca ttcaggacct cacggaacgc
     9781 tccgacgttc ttagggggta tctttgacta cgacgccaag aaagagcgtc tggaagaagt
     9841 aaacgccgag ctggaacagc cggatgtctg gaatgaaccc gaacgcgcac aggcgctggg
     9901 taaagagcgt tcctccctcg aagccgttgt cgacaccctc gaccaaatga aacaggggct
     9961 ggaagatgtt tctggtctgc tggaactggc tgtagaagct gacgacgaag aaacctttaa
    10021 cgaagccgtt gctgaactcg acgccctgga agaaaaactg gcgcagcttg agttccgccg
    10081 tatgttctct ggcgaatatg acagcgccga ctgctacctc gatattcagg cggggtctgg
    10141 cggtacggaa gcacaggact gggcgagcat gcttgagcgt atgtatctgc gctgggcaga
    10201 atcgcgtggt ttcaaaactg aaatcatcga agagtcggaa ggtgaagtgg cgggtattaa
    10261 atccgtgacg atcaaaatct ccggcgatta cgcttacggc tggctgcgta cagaaactgg
    10321 cgttcaccgc ctggtgcgta agagcccgtt tgactccggc ggtcgtcgcc acacgtcgtt
    10381 cagctctgcg tttgtttatc cggaagttga tgatgatatt gatatcgaaa tcaacccggc
    10441 ggatctgcgc attgacgttt atcgcgcgtc cggcgcgggc ggtcagcacg ttaaccgtac
    10501 cgaatctgcg gtgcgtatta cccacatccc gaccgggatc gtgacccagt gccagaacga
    10561 ccgttcccag cacaagaaca aagatcaggc catgaagcag atgaaagcga agctttatga
    10621 actggagatg cagaagaaaa atgccgagaa acaggcgatg gaagataaca aatctgacat
    10681 cggctggggc agccagattc gttcttatgt ccttgatgac tcccgcatta aagatctgcg
    10741 taccggggta gaaactcgca acacgcaggc cgtgctggac ggcagcctgg atcaatttat
    10801 cgaagcaagt ttgaaagcag ggttatgagg aaccaacatg tctgaacaac acgcacaggg
    10861 cgctgacgcg gtagtcgatc ttaacaatga actgaaaacg cgtcgtgaga agctggcgaa
    10921 cctgcgcgag caggggattg ccttcccgaa cgatttccgt cgcgatcata cctctgacca
    10981 attgcacgca gaattcgacg gtaaagagaa cgaagaactg gaagcgctga acatcgaagt
    11041 cgccgttgct ggccgcatga tgacccgccg tattatgggt aaagcgtctt tcgttaccct
    11101 gcaggatgtg ggtggccgca ttcagctgta cgttgcccgt gacgatctgc cggaaggcgt
    11161 ttacaacgag cagttcaaaa aatgggacct cggcgacatc ctcggcgcga aaggtaaact
    11221 gttcaaaacc aaaaccggcg aactgtctat ccactgtacc gagctgcgtc tgctgaccaa
    11281 agcactgcgt ccgctgccgg ataaattcca cggcttgcag gatcaggaag cgcgctatcg
    11341 tcagcgttat ctggatctca tctctaacga tgaatcccgt aacaccttta aagtacgctc
    11401 gcagatcctc tctggtattc gccagttcat ggtgaaccgc ggctttatgg aagttgaaac
    11461 gccgatgatg caggtgatcc caggcggtgc cgctgcgcgt ccgtttatca cccaccataa
    11521 cgcgctggat ctcgacatgt acctgcgtat cgcgccggaa ctgtacctca agcgtctggt
    11581 ggtcggtggc ttcgagcgtg tattcgaaat caaccgtaac ttccgtaacg aaggtatttc
    11641 cgtacgtcat aacccagagt tcaccatgat ggaactctac atggcttacg cagattacaa
    11701 agatctgatc gagctgaccg aatcgctgtt ccgtactctg gcacaggata ttctcggtaa
    11761 gacggaagtg acctacggcg acgtgacgct ggacttcggt aaaccgttcg aaaaactgac
    11821 catgcgtgaa gcgatcaaga aatatcgccc ggaaaccgac atggcggatc tggataactt
    11881 cgactctgcg aaagcgattg ctgaatctat cggtatccac gttgagaaga gctggggtct
    11941 gggccgtatc gttaccgaga tcttcgaaga agtggcagaa gcacatctga ttcagccgac
    12001 cttcattact gaatatccgg cagaagtttc tccgctggcg cgtcgtaacg acattaaccc
    12061 ggaaatcaca gaccgctttg agttcttcat cggtggtcgt gaaatcggta acggctttag
    12121 cgagctgaat gacgcggaag atcaggcgca acgcttcctg gatcaggttg ccgcgaaaga
    12181 cgcaggtgac gacgaagcga tgttctacga tgaagattac gttaccgcac tggaacatgg
    12241 cttaccgccg acagcaggtc tggggattgg catcgaccgt atggtaatgc tgttcaccaa
    12301 cagccatacc atccgcgacg ttattctgtt cccggcgatg cgtccggtaa aataagcatt
    12361 acgttatgct cacaaccccg gcaaatgtcg gggtttttta attgtgctgc gcgaaagcag
    12421 acaacaattt tcttgcttca ctattggctg cctgcatcac catccacgga ctgaacgccc
    12481 acggtgtggc atcaataccg tgtaatacat ctgctaaatc acaccattga taatccatca
    12541 cttcatcatc gttgatctgt aacgcactgg tggtgcgtgc ggcaaatacc ggacacactt
    12601 cattttccac aatgccattc ggatcggtgg cgcgatagcg aaagtcagga tagatagatt
    12661 caggaggcgt aatttccacg ccaagctcat aacggcaacg gcggatcacc gcgtcttcgt
    12721 tgctttctcc cagttgtggg tgcccacaaa ccgagttagt ccacacgcca ggccacgctt
    12781 ttttgctcag tgcgcggcgg gtaactaata attgtccttt ggcattaaac agccaactgg
    12841 agaacgcgag atgtaagcgg gtgtctgccg tgtgtgcggc atacttttcc agcgtacccg
    12901 tgggaactcc ctgtgcattc aataaaatga cgtgttccgt ttgcataatt tctcacatgt
    12961 aattctgatc actcatcatt atagataatt gaagagtgaa atgattaacc aagtgagcgt
    13021 ttatcgacaa ccgcccgttt tgagcggatg ccgacaggta aaaaccattt aaaaaagtag
    13081 catcatatat ttgaagcgac tttcaatggc cattgcaaca cagtcagaaa tgacaggaat
    13141 agagagagta aaaagcaaca gcagactgaa tataacgcgc attaaaaaat tcatattatt
    13201 ctccatgctc atttttcttc agcttgcgca tcagaagcca catacgtact gtcctgactt
    13261 ttaaatcttt atcagagacg gattccttca ccaggtcagc atgatgctgg tagccattaa
    13321 agaagacatt cagcacaacg gcgctaaggg ttgcgagcat aatgccgcta tgcagcaacg
    13381 gttgcagtac ggccggtaac ttagaaaaga aatcgtgaga gagcgtcgga gtcatgccaa
    13441 cgccgagact gatcgccaca atatagaggt tgtaacggtt ggtggtgtag ttacagcgcg
    13501 acagaattcg aatccctgtc gccagtacca tgccgaacat caccagccca gcaccgccca
    13561 gcacaaattg cggaatggag gcgaccagca ccgccatttt tggcaccatg ccaaacagga
    13621 ttaatataat tcccgaagaa atacacaccc agcggctata aatcctttcg catagcgagt
    13681 aatgagcaag ataaaaatta agacagcaaa ggagatacct aaataaacgg gattaccata
    13741 ttgcggattc cctttacctc cggcggccca gtcaataccc acctgaatga tgctaagccc
    13801 aatagaagta ataaccacac cggtaaccag tggcgggaat aaaggcatca agcgaccaat
    13861 aaatggcgct aataatgtgg tgataaaacc cgcggcgata gtggcaccaa atatccccag
    13921 caggccgata tccgggttca tcccaatggc tatcattggt gttacagcag caaaggtcac
    13981 cgacataatc accggcaggc ggatccccat aaagcggccg ataccgatac gttgcaataa
    14041 tgtgacgatc ccgcagcaaa agagatccga gctaatgagc atcgcaatag catcttttga
    14101 gaggcccagt cggtcaccaa tcataagagg aacagcgact gcacctgcgt acatgacaag
    14161 gacgtgttgc agaccgagta tgatcagctt tcctggtgat aatatgcggt caacctcatc
    14221 agttgggcgg tcttgcccag aagatgaggg aagttgggaa tctatggcgc tcatcagtgt
    14281 ttctcctttc gcccgccaga gtaaaaagca accgtttacg ttaatgaaac tgtctaaaca
    14341 catgctcgtg gagtgccagt ttttgcgagg aaggggagtt agagacagga attgcggagg
    14401 caatcacata atcgtgctta aaccacgaaa atgacaagag aacctgacaa tggaattatc
    14461 tgtaattttc tcatattgat atttcattaa tcattctggc atgtcgcact ctagcattta
    14521 atcgttttta tctggatagc gctcttttga tcggcaatgt tgaaataatg aaagcggcat
    14581 agattgaggg gcaataattt gaactcagtc gcaaaattat aattaaaacg gtcatgctac
    14641 ctaatatatg cccgtttatt gcatcgcgaa atttgagaag acataactca ttaaatgaaa
    14701 gggatgcaaa tgaataagtt tatcgctgct gaagctgcgg aatgtatagg ctgtcatgct
    14761 tgtgaaattg cctgtgcggt ggcacacaat caagaaaact ggccgctgag tcacagtgac
    14821 tttcgaccgc gtatccacgt tgtagggaaa ggccaggctg cgaatccggt ggcctgccat
    14881 cactgcaaca atgccccttg tgttacggct tgtccggtta atgctctgac tttccagtcc
    14941 gatagcgtac aactggacga gcaaaaatgt attggctgta aaagatgcgc aatcgcttgc
    15001 ccctttggcg tcgttgagat ggtcgatacg attgcgcaga aatgcgacct ttgtaaccag
    15061 cgcagttccg gcacgcaagc ctgtattgaa gtctgcccaa cacaggcgtt acgactgatg
    15121 gacgataaag ggttacagtg gataaaggtg gctcgccagc gcaaaacggc agcaggaaaa
    15181 gcgtcatcag acgctcagcc atctcgcagt gcagcgttgc tccccgttaa ctcgcgtaaa
    15241 ggcgcagata aaatttcagc gagtgaacgg aaaacccact ttggcgaaat ctattgcggg
    15301 ctggatccac tgcaagcgac ttatgagagt gaccgctgtg tttattgtgc cgaaaaagct
    15361 aactgcaact ggcattgccc gctgcataac gctattccgg attacatccg tctggtacag
    15421 gaaggaaaga ttattgaagc ggcagaactt tgccaccaga ccagttcctt accagaaatc
    15481 tgcggcaggg tatgcccaca ggaccgtctt tgtgaaggcg catgtacttt gaaagatcac
    15541 tctggcgcag tctctatcgg taatctggaa cgctacatca ccgataccgc gctggcgatg
    15601 ggctggcgtc ccgatgtcag caaagttgtt ccccgtatcg aaaaagtggc ggtgattggc
    15661 gctgggccag cagggttagg gtgtgctgat attctggcgc gcgcgggagt tcaggttgat
    15721 gtctttgatc gccatccaga aattggcggt atgctgactt ttggcattcc tcctttcaaa
    15781 ctcgataaaa cggtattaag ccagcggcga gagatattca ccgcaatggg aatcgacttt
    15841 catcttaact gtgaaattgg ccgcgatatt acctttagcg atttaacttc tgaatatgat
    15901 gcagttttca tcggcgtggg gacttacggg atgatgcgag cagatctgcc gcatgaagat
    15961 gcgcccggtg tcattcaggc tctaccgttc ctgactgccc atacccgcca gctcatggga
    16021 ttgccggagt ctgaagagta tccgctgacg gacgtggaag gtaagcgagt cgtggtattg
    16081 ggcggtggcg atacgacaat ggattgtttg cggacttcca ttcgcctcaa tgctgccagc
    16141 gtgacctgcg cgtatcgtcg tgatgaagtc agtatgccgg gctcgcgcaa agaggtggtt
    16201 aatgcgcgcg aggaaggtgt cgagtttcag ttcaatgttc aaccgcaata tatcgcttgt
    16261 gacgaagatg ggcgtttaac tgcggtgggc ctgattcgta ccgccatggg tgagccgggg
    16321 ccggatggtc gccgtcgtcc tcgtccggta gcgggttcag agtttgaatt gcccgccgat
    16381 gttctgatta tggcctttgg tttccaggcg catgccatgc cgtggttgca gggcagcgga
    16441 attaaactcg ataaatgggg cctgattcaa accggtgacg tcgggtattt acctacccag
    16501 acgcatctga aaaaagtctt tgctggtggt gatgcagttc atggcgcgga tctggttgtc
    16561 actgcaatgg ccgcaggaag gcaggcggcg cgcgatatgt taactctgtt tgatacgaag
    16621 gcatcgtgat gaaatcgtta attatcgtta atccggctga ctgtattggc tgccgtactt
    16681 gtgaagtagc ctgtgtggtc gctcaccctt cagaacagga gttgaatgcc gatgtctttt
    16741 taccccggct gaaggtacag cgactggaca gcatcagtgc gccggtgatg tgccatcagt
    16801 gtgaaaacgc cccttgtgtt ggcgcttgcc ccgtgggggc gctgacgatg ggcgagcagg
    16861 tggtgcagac caattctgcc cgttgtattg gctgtcagag ttgcgtcagt gcgtgcccgt
    16921 ttggaatgat caccattcag tcgttgccgg gggatacccg acaacaaatt gtgaaatgcg
    16981 atctctgtga acagcgggaa gaggggccag cctgtgttga atcttgcccg acacaggcgc
    17041 tgcagttgct tactgaaaga gaactcaggc gagtccgcca gcagcgtatt gttgccagcg
    17101 gtgagaatcc tctgtgaaag aaatctccgc gctgaaaggc gcggagaatc tattagattg
    17161 cccaaccacc cgcgtaaaat gcgaccagtg cgacaggaac gctaatttgt tgcccgagta
    17221 ccagactgaa tgcggtaaat gccgtcaagg aaatcgcaca accaatcgcc attggcaaat
    17281 tggcctacaa ccccatcagc aacgagccga agcccgcgac cagacaggtg gcaacaaaca
    17341 cagctgcggg aggaaaacct gctttgccca gcattcccgg aacgacgata acggaataaa
    17401 ccatggccag aaaggtcgtt aagccagcca gtacttcctg acgaacggta ctgccacgag
    17461 cggtaatttt aaaataatta tccagcgcgc cctgtggctt tggtgcgtcc ggtgtttgta
    17521 ggatgtctcc agacataatg atgtcctcac gaggatgtta aagttttatt attagttgcg
    17581 ttcgtacacc agacgaccat caacgtaggt gcggtagatc gaacggtcat cgcccaacgt
    17641 catcatcacg aacaatttgt cgactaaaga aacagagttg tcatagcgca gctgctgtag
    17701 cggagtggcg gtgggttcca tcaccacgaa atcagcctct ttgccaggta aaaagttgcc
    17761 aatcaaatcg tcaaggccca gagatttcgc tccgccgagc gtggccaggt aaaacgcttc
    17821 atatgccgag aggcgatagc cttgtaattg caacactttg taggcttcgt tcagcgtttg
    17881 cagcatgttg aaagttgttc cggcaccgat atccgttccc atgcccactt tgactttctt
    17941 ctgcccagat gccgcatgca tcgtgaattg cggtagcaat cgccggagct gcgccattta
    18001 caccgatttc cgagatcgat ttcgccccga acgggccgac tttatcgtcg ctcggcacca
    18061 gcacagcgcg gaaatcacgc ggaatgtcac caattttcgg tgcgccgtaa ctgcgtaaat
    18121 cacgcgttaa cgggtgacct tcggcgtcat aaatgatctc ttcgctcata ctgtggccga
    18181 tagctcgcag ggtagcaccg tagatttgtc ccaatgctaa ttccgggttg accggtgtac
    18241 cgcagtccag caaggcgtag aatttatcca ggcggatttc acccgtacgc gtgttgacgg
    18301 caacttcagc gaagtttgcg ccatacggga aggcgaaatc aggcgtgata taactgccag
    18361 ttcccaccag tgaaccaaag ccgatgccgg tttcgccttt atgggcaata tccccgaaac
    18421 taacttcgcc tttcttgccg cgcacgacgc ccggcgttgc cagttgaaca tctgccactg
    18481 cctcacctaa catttgcgcg ccgtggaaca gaattttctc ccgtagattt tccgctgcca
    18541 aacgcgccgc gttacccgag aagcaagtac cggacgaggc atatgcgcct ttatcaaaca
    18601 acgcatgatc ggtatcaccg gagataacat gcacgtcctg cggtgggcag tgcagcactt
    18661 ctgctgccag tttcgtcact acagtatcca gaccagtacc aatatccgca ccgccagaat
    18721 gaacgataaa ggtgccgtcc gattccagtt tgatcatgca gttagcctga tcgatatccg
    18781 ggatacccga tttctgcatg ataatggcga cgccgcgacc gatgtgccag tcaccatttt
    18841 gcggtttcgg tgacgaccat tggatcatct cgcgaccctg acgcaggatc tcttccagtg
    18901 cacagctggc ggcggaagga acggaggtcg gcgctttacc ttcaccgatt gcaccgagaa
    18961 ttttcagctc ttgcccttcg tgtacccggt tacgttcgat aatttccagt tggtcgatct
    19021 gtaactgttc agccagttcc gctaatgcca tggtgatagc gaagttacct ttcggtgcgc
    19081 cataaccctg ataagcacca tttgggcaaa tgttgctgta gtaggtggtg acctggaaat
    19141 cgacgttatc gcacggatat aacggcagcg acagcgccgg tccgttacac ggtacggtga
    19201 gtgagtggtt gccgtaaggg ccagtgttgg cgcggaaatc catcttcact gccgtcaggc
    19261 gaccatcttt tttcgctccc agtttgacgg tgactttcgc gacgtgacga gaggtgttag
    19321 caataaactc ttcttcacgg gtgtagcgga atagtaccgg acgcccggtc acgcaggttg
    19381 cccaggcgca cacttcttcc agcaggatgt cctgtttgga accaaaaccg ccgccaactc
    19441 gctctttaat gacatgaact ttatgctgtt tcatgcccac gaggcgcgcg acctggcggc
    19501 gtaagtgcca tggtacctgg gtggaggcgt ggataaccag acgatcgccg tccatccggg
    19561 taaagcagat atgtgtttca gtcgggcact gctgtgcctg cgttgagtta taggttcgct
    19621 caatgatcac atcggcatcg gcaaagcctt tgtccatatc gccaatatga ccatgaatac
    19681 tggcggcgat atttttgcga gggcgagaac cgatcgggaa gttgatgatc atatgctcgc
    19741 cgcgctgggc tgcatggctg ttatcgtctt ccagagtatc tggcgcacca gcaacataca
    19801 ccaccggttc atcgtgcacg acaggcgcat cttccgccat tgcttcgtcg atcgacatta
    19861 ccggcttaag cacttcatat tcaacgtcga tgagcttcag tgcttcgagc gcaatttctt
    19921 cactttctgc gacgaccgca gcaacacgat cgccgacgtg acgcattttc ttgccgaaca
    19981 tacggcggtc aagcggtgac ggttccggtg cgctctgacc ccccggggta tagtagatat
    20041 cagggcaatt caggtgagta ataacgtgaa cgacgcccgg taaggcttca gctttgctga
    20101 catccagatg agtaatcagt gcgtgagcgt gtgggctacg taacatttta atgacgcagg
    20161 cgtcagccgt tacgcagtct tcaacatagc agggtttcgc ctgcaccatt ttcgcggcat
    20221 cagttttagg ataatgcttg ccaatgacgt caaggtcgtc acggaaagtc ggagcgatat
    20281 caatggtggc ctgcggatta tttttacgtg caaccgccag ttcaatgacc tgatagtatt
    20341 gttgccagcc tgcatcgcgg ctgaacaaac cagaaagcgc gtcgtcgatc tcttcgcggg
    20401 taggtgcggc gatgcgatcg agaagatcgg tgataatcag agctgcagct ggatcgttat
    20461 aaccagactg caccacgcca acatcaacca ttgcctgttg aaccagactt aactcgttcc
    20521 atttacccag agattctgcg gtacgaatat ctgccttctc taactgtgcg gcaataagca
    20581 aggacgcgtt aacgatatta ccgttaaaga ttattgcgtc agaaccggca aacccgaagc
    20641 catcatcact gttgcgtaca gagtgcattc ccatgttaaa caacagcttt tgcacgtttt
    20701 cgcctggatt aacggttagc tcctgaggcg cgccatttaa agtaaagtgg atgatcatac
    20761 ggcttcctcc ccagcctgtt ggcagtcggc atacaggtcg gctaccagaa ctcccgtgat
    20821 atagcgttta taggccacgc tgccccgcaa atcttcctgc gggaaaatgg cgttggcgac
    20881 agcctgttca agtgcattgc cttccagatt ttgtttttcg acatcgtgca gacgcagtgc
    20941 tttactggcg acgccatcca gcgcaattcg cataccgtcg tggtctgtca ttgcaacggc
    21001 tgccgtcacg acggttaaac ctgcctgaga acggctaatt ttgcgggtcg cacaggtgcg
    21061 atacggatct ttaatgataa tttcggttag caggcgatcg catgggcagg ccaggtagtc
    21121 ctcgattgac agcgtttcgc cgttgccaaa aaccagttca gcatccagtg ctagcaggac
    21181 gggaagcagc actgactctt cctgccgggc ggcgatttca ccaccaatgg tcgactgatt
    21241 acgaacatgg cgtgagtaaa caaaaccgag ggcttcacgc agtgctgcag gaataaagcg
    21301 cgcatcacgc agtggctgca agcgagacat tgcaccaatc cgcagtgcac cgttatccca
    21361 gtcaacccag tccagttcca gatcctgtaa ggaaatggca atctttttat cggtacgggt
    21421 tggtgtagca ttgagtttgc tgcccccggc gaaccagacg gcttcatcct ggtagcggcg
    21481 cttcagttcc agcgcctgtt cgacagagtc gggcctgaaa aattgttcaa tcatcttctt
    21541 tccttattta accctcttcc ctgcgggaag agggcatctg tcatttatgc cagcgcatcc
    21601 atccgacgcc acatactggc agcggctttt ctggcttgtg cataaatgga atcgcaatcg
    21661 aagttaaact gacggtcttc atagaccatc acaccattga ccatcacgct gtgaacgctg
    21721 cctgagccca taccgaaagc gatatgcccg gcgatattgt ctgccagcag cggcgtcggc
    21781 gagttgtaat cgcaaatggt caaatcagct ttgtaaccgg cttccagaag cccaaatttc
    21841 gcgccaaagt tgcggctcat cagttcgtta ccgttagtca gggctttggc aaaactgtca
    21901 ggccacagcg gaccacccgc atcgcgatgt ttaaagaagg caaatttcat ctcttcaaac
    21961 atgtccgaac caatgccgtc cgttcccaat gccaggttgc ggatgtcgct aaggtgatgg
    22021 ttgtagccga catggttgtt catgtttgaa cgggcgttat gcaccaggaa cgcatcgcgc
    22081 tgattgagta gggcgatgtc atctttcgac aagtacagcc catgagcgac cagcgttttg
    22141 ctgtcgatga ggtcgaattg cgccagtcgt gccagcaggt ctttgccgta ccagtggtga
    22201 ctgtaggaaa cgtcgtaaag gtcttccgca gcgtgaatat gcaaaccacg gcctgtggct
    22261 ttcacggctt cacgcagcat ctccagaccg gcatccggca cggtaaacgg cgcgtgagca
    22321 ccgatatgtg cttccaccag atacggctcg cttgtcgctt tcttcgcctc atcaatcaaa
    22381 cgggcgaaac ggatgttttc ttctacacct tcctgcaact ctttgatgcc gttgttacgg
    22441 tcagtagttt caaaacaggt catcgcgcgc aggccaactt ttaaaaatgc gtcgcgcaat
    22501 gtggagagcg acccgccgat atacgccggc gaggcatggt gatcgataac cgatgtacat
    22561 ccgctcttaa tcgcttccag ggaacaaatc agtccgctgt aatagagcga ctcttcatca
    22621 agggcgcgat cgagccgcca ccagagattt ttcagcgttg agatgaaatc cgggcaaggg
    22681 gcgatgtttg ccataattcc gcgggaaagc tccgagtaaa aatggttgtg cgagcagaca
    22741 attcccggca tcacaatccg accatgcatc tctttgtagc tggcatcggg gtagcgttgc
    22801 gtcagggcat cgccgatagc gacaatcacg tcattttcga tagcgatatc aacgccttcc
    22861 tgcactttcg ccgggtgtaa ctgcactgcg gtgacattct tcagaatcaa catgattaca
    22921 cctccacgcg gcccagcaga taatgatgat gctggtggac atggctgatg atgcggcaca
    22981 tatcgttcag ctccggtggt acgttgttaa actgaccttc gctgtcgatg tttaacaccc
    23041 agctttggtt attcagacgt actcgtaccc ggcaatcttc cacaaggaag cctggggtgc
    23101 tgctgttatc aaagtcttgc gccaggctga agacggtgat tttgtcttcg tatggtttac
    23161 cgttccacgg acagaactga gcgcagttgc cgcattcgtt acagtaagcg tcgaggtgca
    23221 gcgtctggaa tctattctgg aagccaggga ctgcaatgga gacgttggcg cggttcgggc
    23281 agacatccac acacttgttg caaacgtagt tacattcgag gcagcgagcg gcttcctggg
    23341 cgacaaacgc gtcacgatcg tcactgttca ccagcgtgat agagatatcg cctttacgtt
    23401 gatagatttc cgctggattg acgttgttcc agtatttatc gttctggtgg gaacggatat
    23461 tttcccgact aaggatggca tcggtcgccc gacgcgcggt tccgacagca gcgacaatgg
    23521 aggatggtcc gcgctgtacg tcgccgatca taaagacgtc agtcagacga gtttcgccat
    23581 tatggtcgac gtctggccag ccgtttttgt ccagcggcac gcccatcgca ttcagggctt
    23641 cagtatcctg ctgttcacca atggcggtga tcaggctgtc tacatgcagt gttactgttt
    23701 cgttggtttc aaccggacgg cgacgacctt tctcatccgg ttcgccaagc gacataacgc
    23761 gcaaggttaa ggtgccatca gcatcgaaac gttccggatt attcaggaaa cggaactcta
    23821 cgccgtcgtg caacgcttct tcatactctt cgcgccatgc gggcatctct tgtagtgaac
    23881 gacggtaaac gatcgttgct ttttctacgc ctggaacgcg taacgccgca cgagcgcagt
    23941 ccattgcggt gttacccgcc ccgacaacga ccacatgttt gcccagcttg agcgctgtac
    24001 ccttgttgta ttcacgcagg aaggggagtg atttccagac attttggttg tcgcccgcca
    24061 gtttcacacc gctattttta tcagtgccgg tggcaatcag aacatagtgg aagccctgat
    24121 tttttaactg ttcaacggtt aaatcgggtg agcagccata ctcaaatttc acgccgtgag
    24181 cggcaacaaa atcgatatcg tgctgaatta actctgcagg aatacggaac tgaggaatga
    24241 tatttttcac cacgccgcca gcattggctt cgcgttcaaa cagcgtaacc ggatggcccg
    24301 ctctggcaag gaagtaacct gctgccagac ccgccggacc tgcaccaatc acggcaaccg
    24361 gatggcgtga accagaaccg gcgggtttgt gccagcgttg cttatattca tcccaacctt
    24421 tttccagcgc gacttttttc agttcgcgga tattcagcgc actgtcgtaa tccaggcggg
    24481 tacagttgta ttggcactgg tgatcgcaaa tatgaccggt aatggcaggt agggcgttgc
    24541 gttggtagat gagttccagc gcgtcggcat agcggtgttc gccaagcaga cggatgtatt
    24601 ccggaatatc ttgcttgata gcgcaggcag taacacaggg ggcaacgtag caatcggtca
    24661 gcggcaggtc ttctgccact tcaatacgct cttctggctt ccagtgtttc tgggtgtatt
    24721 ccatggttaa cgcgtctgct gccagtctgt tcagtcgttc gacgtcaaca tggtcaagtc
    24781 cccaggcgtc ggagccttcc agctcgcgca tgcaggcact taagcgcaga tagccgccag
    24841 gtttcagcag gtcggttgcc atagtaatag ggcgaatacc tgtatcaaaa atatcgcgga
    24901 tagtcagctg actggcacca ccggaataag aaatgggcag tttgccgtca aaggcgcgag
    24961 agagaactgc tgcaacattg atggagagcg ggaacagcgc acggcctgac atatacatct
    25021 cttcaccagg cagtgcgcct ttattgttga tggtgccgag agtgttagtc agttttacgc
    25081 caaagccgag tgatttttct tttgccagta ccatcaggcg ctccagcatt tccagtgctt
    25141 gcgtcagctt gaggtcgtga tcaaatgact cttcttttaa gcctatgtaa ccgaaaccgc
    25201 agacatcgag gatctcacga acacgcgcgt accccagtaa ggtcgggtta agtttgacaa
    25261 aggtgttgag ccctttttct tccagcatgt agcggcaaat ggcttcgatt tcatgcggag
    25321 gacagccgtg catggtggag agggtgacgc cctgcaccat actggtgggg atgcgagcgg
    25381 gtaaggcttg caagttttcg cgtttttcct gcaatccgtg gcgggctaaa aatgcgtcat
    25441 cctggagtaa tttattcagc gtatcgcgat attgagcgaa tttcggatgg tcagatgcgt
    25501 ccatcatatt gtcgatgaac tgttgcatcg gcggttgctt aataccttcg aggttgtaac
    25561 cgacgctcat attaaagatg aacgatttac cggaatcaga aggctgtaac atcgcttcga
    25621 gaaggtgcag ggcaaaccag gctttgaggt attcatccca ggctttaagc aaggtaaatt
    25681 cggtagacca ttcggtgtta aagcattcgt cttcggcatc aatacagggc ttttccagct
    25741 ccaggcggtc aagaatttgg acggttttta gttcgatgaa gcgtccgcca gtcagccagg
    25801 aagtgacaat attttgcgcg agctgcgtgt gcgggccagc ggcagggccg acgggagtgg
    25861 cacaggtttc accgaagacg ctaacagttt taccttttac aggtgagtaa aactgttgct
    25921 cgggaatacc aaagattgag cgttgttgtt ggtattcatc aaatatgcgc gtcaaaagtt
    25981 cctcaaacgg aatgggacgc ataatatccc ccataaccct ctccttagct tatacagata
    26041 tcattgagtg accggtgttg agaaatccgg tggtttaggg ggatagagca acaatcgcac
    26101 cactcttgcc aggtttggcg ggaaatataa aaaaatgtga ggtggctcga aacttaaagg
    26161 ctttttgtga atgaactcac aggtgccaaa tgagaattaa aatcactcct gaaaattatt
    26221 attgtcagcc catttatcac tttttctctt ttctcaaaat cagtgattcc ttatcattat
    26281 gataaagagt gctggagaga ttaaatattt gaacgcaatc gtttaactac attttctaaa
    26341 ttttagtata ccttttttgc gctgtaataa agtcgtccgg agtatcaata tctaaaatta
    26401 tttctgcatt ttctatttcg acggaataat gatctcccat aaggagtgcc tgacgcatat
    26461 tggtgacatt gggtcgctgg attgcctgca tcaggcatgg ttttgacact aaaatcggat
    26521 ggccggggat gccattatgg agtggcagta ttgcgccatc atttcgtaac gaccagattt
    26581 ttctaaaaat atcgatggtg agggttggca tgtcaccgtg ggtgagaaaa caatgttctg
    26641 tttgcactgc gggtactgcg gccttgacgg atgttagtaa accctgcgca taatctgggt
    26701 tgtgaataat agtgatattg ctctggttcg cataacgttc gtgcagttca ttaccacgat
    26761 agccggtgac taaaataatt cggctacaaa actgcaacgc atttttgata cttgtatcaa
    26821 gaattgttcc ctgttcccag gggaacatca ttttccattg ccccattctt gatgataatc
    26881 cggcagcggt aattatacag tcgatggctg acatagtggt tccattattt ataggggcta
    26941 ataacgcctg ataattgcgt tacttcctga tggattaagt tggggataat ttatcccaga
    27001 gaggtcataa agactcgtga aaagtatagt tgacccatcg gcattagtca ttgatttagg
    27061 tgcgcagaaa cgtcccactg tgatttctgt tgttggtgct ggggggaaaa ccagcttgct
    27121 tttttggctg gcagaactgc tccaggctag cggcaggcgt gtgttaatca cgacgacgac
    27181 acatatgttt atgccaacgt ctcactggcc cgtggttttc tgtcgtgatc ccgccatgct
    27241 tcctcatgcg tctcttacat cccccatttc attttgtttt cacagctgga aagcgaacca
    27301 gggaaaagtg cagggattta cgccagaagc gattgatgca ctggtgcaac gaccagagtg
    27361 tgacgtaatt ctcattgaag cagatggctc gcgtggaatg ccgttaaaag cgcctgatga
    27421 gcacgaacct tgcataccca aaagcagttg ctgcgtgatt gctgtgatgg gaggacatac
    27481 tttgggcgcg aaagtgagca cagaaaatgt ccatcgctgg tcgcagtttg ctgatattac
    27541 cgggttaaca cctgatgcaa ccttgcaact gagcgatctc gttgcgctgg ttcgccaccc
    27601 tcagggggcg tttaaaaacg taccgcaagg ttgtcggcga gtctggttca ttaaccgttt
    27661 ttctcaatgt gagaatgcga ttgcacaaag cgagctcctt caaccgctgc aacaacacga
    27721 cgtagaggca atctggctgg gcgatataca agaacatcct gcaatcgcgc gcagatttgt
    27781 gaattagcgg cccgggtatt ccgggttact gaaaggatat ccattgaggt tggtatgaat
    27841 attttcacag aggctgcaaa actcgaagag caaaattgtc cgtttgcgat ggcgcaaatt
    27901 gttgatagcc gaggctcgac tccccgccat tctgcacaaa tgttagtgcg cgccgatggt
    27961 tctatcgtcg gtacaattgg tggcggaatg gttgagtgga aggtgattga agagtcactt
    28021 caggcattgc aggaacgtaa gccgcgatta ttccatggac gtatggctcg taacggtgcg
    28081 gatgctgtcg ggtcagattg tggaggtgca atgtcagtat ttatcagcgt ccatggtatg
    28141 cgtccacgtc tggtgttgat tggcgcgggg catgtcaacc gggcgatagc ccagagtgcg
    28201 gcgctattag gatttgatat tgccgttgcc gatatttatc gcgaaagcct caatcctgaa
    28261 ctattcccac catcaaccac gcttctccat gctgagtcgt ttggtgcggc agtggaagaa
    28321 ctggatattc gccctgataa ttttgtcctg attgccacga ataatcagga tcgtgaagcc
    28381 ctcgacaaac tcattgaaca gcccattgca tggttggggt tgctggcaag tcgccgcaag
    28441 gttcagcttt tcctgcgtca attgcgtgag aaaggcgtgg ctgaagaaca tattgcccgt
    28501 ttacatgcgc ccgttggtta caacataggt gcggaaacgc cgcaggagat cgccatcagc
    28561 gtgctggcag aaatattacg ggtgaaaaat aacgcgccgg gtgggctgat gatgaaacct
    28621 tctcatcctt ccggacacca gctggtggtg attcgcggtg cgggagatat cgccagtggt
    28681 gtggcgctac gtctgtatca tgcgggtttt aaagtgatca tgctggaagt ggaaaaaccg
    28741 acagtgattc gttgtaccgt ggcgtttgcc caggccgtgt tcgatggcga aatgacggtc
    28801 gaaggcgtca ctgctcgcct ggcaaccagc tctgcggaag cgatgaaact taccgaacgc
    28861 ggattcatcc ctgtgatggt agatcccgcc tgttcattgc ttgatgaact gaaaccgctt
    28921 tgcgtggtgg acgctattct ggcgaaacag aatttgggaa cgcgggcaga tatggcacca
    28981 gtaacaatcg cgcttgggcc gggctttact gcagggaagg attgtcatgc ggtaattgaa
    29041 acaaatcgcg ggcactggct cggtcaggtg atttactctg gttgtgcgca ggagaatacc
    29101 gatgttcctg gcaatattat ggggcatacc acccgacggg tgatccgtgc tcctgctgca
    29161 ggcattatgc gatccaacgt gaaattaggc gatctggtga aagagggcga tgtgattgcc
    29221 tggattggtg agcatgaaat taaagcaccg ttgacgggga tggtgcgtgg cttgttgaac
    29281 gacggcctgg cagtggttgg tggttttaaa attggtgata tcgatcctcg tggtgaaacc
    29341 gctgatttca ccagcgtttc tgataaagcc cgggcgattg gcggcggcgt acttgaggcg
    29401 ttaatgatgt tgatgcatca gggcgtgaaa gcgacaaaag aagtgctgga agtggcttaa
    29461 atgaaaatgg ccggatggta ttacccaccc ggccttaaaa ttactgacaa atcaccgttc
    29521 cggttttgcc ttcaatccct tctttcgctt tgctcaatac ggtaatcagt gcttcacgac
    29581 ctgcgcgaga gcgagcaaac gaggcggcag cttccacttt aggcagcata gaacctttcg
    29641 caaaatgacc ttcttcgata aagcgttcag catcacttaa tgacaggcga tcgagccact
    29701 gttcattctc tttaccgaag ttaatggcca ctttttctac cgcagtcaga atgatcagca
    29761 tatcggcatc aatcatttct gctaaacggg cgctggccca gtctttatcg ataaccgcgc
    29821 tggcaccacg cagatggtta ccttcacgaa taacagggat accaccaccg ccaacggcga
    29881 tcaccacctg gcctgcatct accagagctt taaccgtttc tttttcaatg atatcaaccg
    29941 gttttggcga ggcaacaaca cggcgatagc cgcgaccagc atcttctttc agagtgtaac
    30001 cctgttttgt cagttgctct gcttcttgct cggtaaagaa cgaaccgatc ggcttggtcg
    30061 ggttgaggaa tgccggatcg ttagcgtcga cttcgacctg agtaaccagc gttgctacag
    30121 gtttattaat gccccgagaa agcagttctt ctcttaacgc gttttgtaga tcgtaaccaa
    30181 tataaccctg gcttaaggca acacagacag acatcggcag cattggcgag tgtgcttcag
    30241 tttttgcggc agcttcaaag gcttggttaa tcatgcccac ctgcggcccg ttaccatgag
    30301 tgacgataac ttcatgtcct tgggcaatta aatcaacaat tgcctgagaa gtaattttta
    30361 ccgctttcat ttgtccggcc aggtcatcgc ccagcgcatt cccgcccagg gcgagaacaa
    30421 ttttcttact catttttcct cactcatcat tcttgattgt gtatagatcc ctgacagtgg
    30481 ttagagcacg ggagggacaa acggtttgcg tcgcaggaaa cgacctcgcc cggctttgcc
    30541 tgtaaaggtg ccgtcacaga aaatcgtttc accacgggat aaggttctga caatcgcgcc
    30601 ctgacaggta aaaccctccc atggcgagta gtcggcgttg tcgtggagat ggcgatgctg
    30661 aatttgttgg ctctgacgtg ggtcgataat caccacgtcg ccatcggaac cgggcgctaa
    30721 taatcctttt tgcggccaca ggccaaacag cctggcgggc attgcgctgg ttaattcaac
    30781 aaagcgttcc ggtgttatac gtcccgtcat cacgccactg gaaaacagta actgcatgcg
    30841 gttttccaca ccgggtaagc catttgggca gcgactgaaa tcgcctttag aaatttgcag
    30901 gcgttgagcc atcgagaagg tgcagtgatc ggtcgccacc acgtcaatcg caccatcgct
    30961 gatgccacac cacagtttgt cctgctcgcg tacgttacgc agcggtgggc taagaatgaa
    31021 cttcatgcca tcttctgtat cgtaactgcg ttcgtccaac aggagatatt gtgggcaggt
    31081 ttcaacccag actggctggt gattcgcacg ggcaagacgc agataatcca gacctaagcc
    31141 gttagacagg tgcacgatat agagcggggc gttaccggca atttgtgcca ggttaatcat
    31201 gcgggcgatg gcttccgctt cgcattccag agggcgactc aaggcatgat agcgcggcgc
    31261 ggttaaccct gcggcgataa attccgcccg cttgctggcg atagccgcat cattttccgg
    31321 gtgcacggtg gtcagcgcgc cggattcatg caagcggcgt aatgcctgca aaacctcgtc
    31381 atcgttgagt ttgtattgat aggttaaata gagtttaaaa ctgctcagtc cttcctcgac
    31441 catcatcggg atttcgtcga ggattgcgtg attaatgtgc tggatcacac cgtgaaagct
    31501 gtaatcgatg accgctttat gggcggcata accacgataa acctccagtt gatggcgtaa
    31561 ccgacagccg tttgggccaa atcccatatg gtcaataatg gttgttgtac cgccacacgc
    31621 agctgcgcgg gtaccggtaa aaaaatcatc acaactgcgc gcgatgccga catcaatatt
    31681 gaaatgcgta tggacatcca cgccgccagg gaaaacgtaa cagccagtgg catcaatttc
    31741 ttcatacggg agctgcggcg aaatattgtt gcccaactgg cgaacaatcc cgctttcaat
    31801 cagcaaatcc tgtttggctt gtccatctgc gttaacgaca gtgccgtttt tgatcaatac
    31861 gcgcatagca aactccaaaa accctccggc gaaccggagg ggaataagtg aagagtaatt
    31921 actctgttgc cagccagctt aacgggattg cagcgtacat cgcggcacag gtcaccaggt
    31981 gagatttcca ggttttttcg ttaggtgcat gcgcttcagg ttctttaccc gggccaaagc
    32041 cgatcaccgg aatgccgtga cggcccatga tagatacgcc gttagttgag aaggtccact
    32101 tatcaacaac cggcgctttg ccaaacagcc cttcgtaggc attcaccagt gctttaacgg
    32161 tgaagtgatc ttcttccact ttccaggtcg ggaagtagca ttcggttggg taaaccaggc
    32221 cagtccagga cggacggtcg tagttgtaca tagaaacaac cgcgttagct ttctgtactg
    32281 caggcagggc gcggatttcg tccagcgcgc cttcccaggt ttcgccccag gtcagacggc
    32341 ggtcaataga gacggcgcag ctatctgcta cagcgcaacg gcttggggat gtgaagaaga
    32401 tttcagaaac ggtgagggtg cctttgccga ggaattcgtc ataacccaga cgttgggaga
    32461 gttcttgtaa ttcgccaaga atcggaccca ttttgaaaat ggcgttgtca ccgcgttctg
    32521 gcgcagaacc gtggcagcta acaccctgaa catcaatacg aatttccata cgaccgcgct
    32581 gaccacggta tatctggcag tcggttggtt cggtactgac cacaaattcc gggcgaatgc
    32641 cggattgttc aataatgtac tgccagcaca gaccgtcgca gtcttcttcc tgcacagtac
    32701 cggtaaccag cagggtatat tcatcttcca gaccgaggtc tttaatgatt ttaccggcat
    32761 aaaccataga tgccatgccg ccttcctggt cggaagtacc gcgaccaccg attagctcat
    32821 cagtttccat gccttcgtac ggatcgaagt cccagttttt gatgttgcca atgccgacgg
    32881 tatcgatatg agcgtccatt gccaccagac gcgggccgtg gccgatataa ccgagaacgt
    32941 tgcccatcgg gtcgatttca actttatcga agccgacttt ttccatctct tctttaatac
    33001 gatgtactac gcgtttttcg tcgcagcttt cactgggaat agcaaccatg tcgcgcagga
    33061 agcgagtcat gtccgcctgg taatcttttg ctttttcaag aatcagtttg aatggaatat
    33121 tcttagccat tatttttctc cagatttttt actccccgtt ttgcagggag tagttccaat
    33181 agggtgatta aggtgctaca gcgtgtttgc cttcccagac aacttcgcgg tagtgcttca
    33241 cgtcggtgtc gccttcagtg ctgataacca gcactacggc atctttgtcc agcgccagtt
    33301 tttccatcag gctttgatgt tgcgggtgat aatgaaccgc tgcgagaacg cccaaaccga
    33361 cagcgccgga ttcaccggag atgatgcgcg ggtcgttgcc gtacggatta cccagcacgc
    33421 gcatacctaa tgcggcaacg ctgtcctggc aggagatgaa ttgggtggcg cagttacgta
    33481 ggatttccca gcccagcggg ttaggttcgc cacaggccag gcccgccatg atggtggcca
    33541 tatcaccgcc aacgttgacg atgtcgcctt tgacgccgga gcgataaata cagtcagctt
    33601 tgtcaggttc aacaataatg ctgtgcagat tttgcgggct gtagacgtcg accagataac
    33661 ccagcacacc accggccatt gctccgacac cggcttgcag cagaacgtgc gtcggggtta
    33721 cgcccatttc acgcatttgc tcgacggctt catctgccag ggttgcgtag ccttgcatga
    33781 tccaggttgg gattttggtg taaccttccc atgccgtgtc ctgtaccact tcccagccgt
    33841 gctgctgcgc gtgttgcatg gtcaggcgaa cggtatcgtc atagttcata tccgtgacga
    33901 tgcactcggc accgaggttc agaatggcgt caacgcgttc ctgagcagaa cctttcggca
    33961 tgtaaatcac cgcattctgt ccgagttgct gtgctgccca cgccacaccg cgcccgtggt
    34021 tgccgtcggt ggtggtcgcg aaagtcattt tttcgccgat ggcatttttc aggtgctcaa
    34081 atgacagcgt ttcgatatca agatgatatt tttcacacaa taattgagcg atggcgtacg
    34141 caccgccaag cattttgaag gcgttcagac cgaatcgttt tgattcgtct ttgacgagaa
    34201 ttttcttcac accaaaaagg tttgcgagat cgtccagcgc acaaagcggt gttgggcgat
    34261 aaccagctat tttctggtgg aactggcgcg ccagtttggc ctggctttgc gaaaagagcg
    34321 gtgatgtttc gccgttgaaa aatttgttat cggcgatatc aatcttcaat gagaaaacgg
    34381 acatatccta tccttttggc ctctggaaat agagggccga ccagcagatc ggcccgaggg
    34441 gttatttcac gcgttcttgc gcttctttca gcagttgctc gagcagtgca cctggtttgg
    34501 cgtatttacg ggacaggatc atcgcggcga tgatataagg cttccagctg gcttctttgt
    34561 aggtagcgat acggtatttt tcgaatacgc cttcagtcac ttcaccttct ttgcaggata
    34621 cgccgctgat atcagctggc aggcagtgca tgtacagggc ttcgccatca cgggtcagtt
    34681 ccatcatctc ttcagtacaa tgccagtctt tgtgttgcgc gttctgtgcc agacactgtt
    34741 tttccagtgc ttttaagcct tcgtggtcgt tcgcacgcag caattcagta cgctcttcca
    34801 tcactttgta aggtgcccat gacttcggat aaacgatgtc tgcgtctttg aaggcttctt
    34861 ccatgctggt gacctgacgg aagctaccac cggaggcttt agcattgttt ttcgccactt
    34921 caaccacatc cgggatcagg tcgtagcctt ccggatgggc caaggtgaca tccataccga
    34981 agcgagtcat cagaccgatg atgccttgtg gtacagagag cggtttgcca tagcttggag
    35041 agtaggccca ggtcatggcg atttttttac ctttcaggtt ttccagtgaa ccaaagtgtt
    35101 cacgtaacca cgcgaggtca gccattgact gagtcgggtg gtcaatatcg cattgcaggt
    35161 tcactaaagc cggacgctgt ggcagtacac cctgcttgta accgtcatca agtgcagcgc
    35221 caacttcacg catataggcg ttgcctgcgc ccagatacat atcgtcgcga ataccaatag
    35281 cgtcggcgca gaaggagatc atattggcgg tttcacgcac ggtttcgccg tgagcgattt
    35341 gtgatttgcc ttcgtcgaga tcttgttgtg caaggccgag caggttaagc gcggaagcat
    35401 aagagaagcg ggtacgggtg gagttgtcgc ggaatacgga aatacctaat ccactattaa
    35461 agactttggt tgagatgttt tcagcacgca gtgcttttaa tgctgcggca acgtccagta
    35521 cttgtttcag ttcatctggc gtctgttccc acgttaacaa aaaatctttc tcgtgaaggt
    35581 gagaggtcag cgaattgata tccttaatca gctcattaac agttttcatc atacagtccc
    35641 ggtagtggaa tgacaggcaa agtggtttat ttcactgtga cttgccaaag tgattggtgt
    35701 tttcatagct atacctggga ctaacaataa gtgtgccaga attatccgcg cggctgcaaa
    35761 agactaattt gacggataat gtgtggggtt gttcacaaat tgatgccagc ttctggttgt
    35821 ttgtaaaata cgaaaacgtt tggctttttt tatcgcgtta tttatttgct gctaagtatc
    35881 agaaataaga taaaggggca ggctatctct cttcagtttg ataaactcac cgccgtaatt
    35941 ctcatattga tatagaaatg tgatatctct tccataattg cattagaaaa tgataaaaat
    36001 cttatgtttt tatcaaagcc aatttcgcct gagttaatat catcatgatc tgtgaaccat
    36061 caacgtcttc aatgatcgac ttttaagtaa gtctggaagg taaagctatg gagcttgcta
    36121 ctacgcagtc agtattgatg caaattcaac cgacaattca gcgttttgcc agaatgcttg
    36181 ccagcgtttt gcagtttgag gttgagatcg ttgatgaaaa cttgtgtcgc gtcgccggaa
    36241 cgggcgcgta tgggaagttt cttggtcgcc agttgagcgg caactcacgc ctgctccgcc
    36301 acgtcctgga aacgaaaact gaaaaagttg tgacacagtc tcgcttcgat cccctttgcg
    36361 aaggttgcga tagtaaagaa aattgccgcg aaaaagcatt tctgggtacg cctgtcattt
    36421 tacaggatcg ttgtgttggg gtgataagtt tgattgccgt tacccacgag caacaagagc
    36481 atatcagtga taatttacgc gaattttctg attatgttcg ccatatatcc accatttttg
    36541 tttcgaaact tctggaggat caggggccag gagataacat cagtaaaata ttcgcgacca
    36601 tgatcgataa tatggatcag ggcgtattag ttgttgatga tgaaagtcgg gttcagtttg
    36661 ttaatcagac tgccttaaaa acacttggtg ttgtacaaaa taatattatt gggaaaccta
    36721 tccgtttcag accattaaca tttgagagta attttactca tggacatatg cagcatattg
    36781 tttcgtggga cgataaaagt gaattaatca ttggtcaatt gcataacatt cagggccgac
    36841 aattattttt aatggcattt caccaatcgc ataccagttt ttctgtagca aatgcacctg
    36901 atgaaccaca tattgaacaa ttggttggcg agtgccgtgt tatgcggcaa ttaaaacgac
    36961 tcattagccg tattgcaccc agcccatcca gcgttatggt ggttggtgaa agcggcacgg
    37021 gtaaagaagt cgtcgcccga gcaatccata agttgagcgg aagacggaat aaacccttta
    37081 ttgctatcaa ctgtgccgcg attccggagc agcttctgga aagcgaactg ttcggttatg
    37141 ttaaaggcgc atttactagt gcttctgcca acggtaaaac agggttgatt caggcggcga
    37201 atacgggcac gctgtttctc gatgaaatag gtgatatgcc attaatgttg caggctaaat
    37261 tactgcgcgc tattgaggcg cgtgaaattc tgccgattgg tgccagtagc ccaatacaag
    37321 tcgacattcg catcatttct gcaactaatc agaatttggc ccagttcatt gccgaaggta
    37381 aattccgcga agatctcttc taccgactta atgttatccc gataactctg ccaccgctgc
    37441 gtgaacgtca ggaagatatt gaactattgg tgcattactt tttacatctg catacccgtc
    37501 gtctgggatc ggtttatcct ggcattgctc ccgatgtcgt cgaaatattg cgtaagcatc
    37561 gttggcccgg aaacctgcgc gagttaagca atttgatgga atatctggtt aacgtggttc
    37621 cttcaggtga agttatcgac agcacgctat tgccgccaaa tctgctgaat aatggcacaa
    37681 cggagcaaag tgatgtaaca gaggtcagtg aggcgcacct gtcactcgat gatgcgggcg
    37741 gcacggcgct ggaggagatg gaaaagcaaa tgatccgcga ggcgctttca cgtcataaca
    37801 gcaagaagca agttgctgat gaactgggca tcggcattgc tacgctctat cgcaagatta
    37861 agaaatatga gttgttaaac acataacctc agtgaatccg gcctgaattc aggccggata
    37921 tccttttact tcgttttctc gcaatccaga actgtattta caatcatctg ataccccgtg
    37981 cagcgacaaa gatttcccgc cagtccgcga cgaatttccg taatggttaa tggcttctcg
    38041 cgtggtttcg ccagcattgc cgtggtagcc ataatcaggc caggcgtaca aaacccgcac
    38101 tgcactgcgc cggatttcgc ataagcctgc tgaacatgag aaagttttcc gcctttcgct
    38161 tcaccttcca gcgtgcggat ctcctttcct tcagcccagg cggcaaggta taagcaactg
    38221 tctattgctg tgccgtcgac caacaccgta caggcaccac attcacccac gcagcaccct
    38281 tgtttgacac ttagcagtcc ttgttcgcgg agtaattccg agagcggcgt gcctggtgcg
    38341 gcgtgaagct gaaaaggcat cccgttaatg gtgcattcga tggtaattgt ttcgctgtga
    38401 ttcattgcaa ttttcccccc gccgcggcga cggcttcgct aatcactttt ttggtcatcg
    38461 tctggatgag atgcagacga aactctttac tggcccgcca tgaagaacgc ggggcgacat
    38521 cttgcaggac agattcgctg atagcttcca gcgtttgcag gtttaatggc gcattttgtg
    38581 cagtctgttc ggcatgttgg cagcgaatcg gcgttggcgc ggcaacacca aatgccaggc
    38641 gtaattcgct gaaattgccg ttatccagtc ggcaatgtgc ggcgcagcca atcgttgaaa
    38701 tatccattgc gtcgcgcatg gcatatttaa aatgcgcgct gcccgcgtgt tctttcggct
    38761 gtggcggaaa atgaaaggcg acgaggattt cgtcatgctc aagagacact ttgcccggcc
    38821 cggtgtgaaa gccattaatc gggacgaaac gaacaccgcg cggggagtgg atctccagtt
    38881 tcgcgtcata aattagcgtt ggcgtggcag aatctgcgct ggtggcaccg ttgcaaatat
    38941 ttccaccgta ggtagcgacg ttacggatct gcggtccagc aatggacgtg gccgcagcac
    39001 ataacgccgg gagatgacgt tgagttatag gagcttctat tagctgggta aatgtcgttg
    39061 cagagccgat acgtagcgag ccatcttccg ccagcgtaat tccccgcagc tccgccagat
    39121 tatgaatatc aacaatatgg cgataacggt cattgtgatg gtggagctga atcagtacgt
    39181 cagtgccatc ggcgagcagt ttggcctgcg ggttgtcagc cagcaggttg atggcatcgg
    39241 caagggttgc tgcgcgatgg taagaagcaa aatcaaacat gatgttatcc tcaaatcaat
    39301 cctgccagat ggaactcttc atataaccgt tttggcgtca gcggcagtgt attgattgca
    39361 acaccggtag ccatcttcac cgcgttacga atagcagcgg caacaggaat tattggtggc
    39421 tcacccagtg acttatgtcc gtatgcggat tgcggctcat tgatttcgac gaacgcgctt
    39481 tccagttgtg gcagatccgg catggtcggc attttgtaat ccagcagatt ggggttacgg
    39541 accacgccgc ttttagcatc gatgatcatc tcttcaaata gcgcccagcc aatgcccatt
    39601 cccattccgc cgtgtacctg accttctgcc agcagtggat taagaatatg ccctgaatca
    39661 tgaacgttga ggatgcggtt gatggtgact ttgcacagcg caatatcgac cgtcagatca
    39721 acaaaggtac agccaaacgc cgatgggtta gtggtggttt tgatggagct ttcagcagag
    39781 agctgcccgc cgcgttcagg gtggtagaaa gcgtccatcg ccaaatcttt taacgacatt
    39841 aacggctctt ccggtcgttc aaccagcacg atatggcctt ttatcagggt cagattcatc
    39901 gctgactgat gtagcatgac tgcggcgtga gcgatgattt tctcttttaa taatagtgcc
    39961 gcactgcgca gcgcaggcgc ggcaacatag ctctggcgtg aggcaaatgc gccgggagcg
    40021 aacggcgtaa cgtcggtatc ttgagttgaa ataacgcgaa cgtcgctgac cggaaccccc
    40081 acggtttctg ccaccatttg cgagaagacg gtgtcggcac cctgaccgat ttccgtcgcg
    40141 ccgctttgca cgttgatggt tccatcctga ttcatcagaa ggcgcgcgcc tgctatttct
    40201 acgccgacag gccaggtgtt agaggtatag ctaaaacagg cgacgccaac gccgcggcgc
    40261 aaattgcctt gctggttctg gcattctgca cggcgttttt cccattcaaa gattttccgg
    40321 cctttttcaa gacactccgg caaccctgcg ctgtaaatac gtttgcccgt gagcggatta
    40381 gcatctcctt cgcgggcggc gttgcgtaaa cgaatttcaa caggatcaat acctaacgct
    40441 gtcgcggcgt catcaagcat agactcaacg gcaaatacga cttgtggcgc gccataacca
    40501 cgcatcgcac cagccgaggg gaggttggta tagcaggtct ttgaactgta agcgtaggca
    40561 caacgaggat aaaggtaagc gactttattc ctccccgcag aagcgatgga gtgcccgtga
    40621 gatgcataag cgccggtgtt agacagaaca tccagactat aacctttcaa tgttccgtcg
    40681 cggttcacgc ccatttgccc gtcaatggta aaagcgtggc gggtacgggt tgcgaggaaa
    40741 cactcttcac ggctaaggga aactttcacc ggaatgccgc caagcttgct ggtcaggaat
    40801 gctgccattg gctcttccag tacatcctgt ttattaccaa aaccgccacc gacaaacggt
    40861 ttgatgactc gtacgcatga ccagggaata tccagcgcct gaccaaccac gcggcgaaca
    40921 atgtgcggga tctgggtgct ggaaacgatg gtaattcgcg agtcatcctc catccacgcc
    40981 agcgatgtta cgctttccat gtgacaatgt tgaataacgg gggtctgata gtgcccctgt
    41041 acctggtagt cggcggcatc gattgtttgt tggacattac ccgtcgacat cgtgctttgt
    41101 ttcagtaaat tgccaccgtt atggattggt gcagcgtctt ctgccagcgc cgcttctggc
    41161 gtggtgataa cgggtaattc ttgccactca atgctgacca attgcgccgc tttttctgcc
    41221 gtgagttcat cgcgggccac gacgatggca acggcgtcac catgatgacg aacatggcga
    41281 gttagcagtg cgcgatcggc ggtatcgcgc ttgttttcgt caagtgtcca ggcatgccca
    41341 gctgtagcga atggaatatc aggcacatct tcccaggtaa aaatcgccag tacgcccggc
    41401 aaacttctgg cttgttcatc attaatactt acggcataac catgtgcgat agggctacgt
    41461 acatatttcg cataacacat gcccgccata acataatcgt cagtatatcg tgcccgcccg
    41521 gtgaccttag caatggcatc gacgcgcatg catgattcac ccgtagcggt tgcttcccgc
    41581 gcttccatag aaatcccctc gatttgaaca tttggttctt atggcaaaga tttagtctaa
    41641 gcaggcaaga tttacgccag aaacgacgat aagcgttagg ggaggtagga agtgtgatga
    41701 cgttaaaaaa acacagatat gatacgaaac aggactctga ataggggcag tgatacgtat
    41761 caatatgagt ggcgataaaa tcaatgtgat aaattgggat tattacgcat caggatggga
    41821 aatagggtaa aaagtcagga agttcgatac ctctacagcg aatattttgc tgatgatact
    41881 tccctttgcc aaatgaaatt aacctctttc tctattacca cgtttttcca gaagcaagag
    41941 attgggtatc aagaggctgg ctgctatgat aaggcgtctt gttttttaag cgaggaaaga
    42001 ttttgagtgc gggacgcctg aataaaaaat ctctgggtat cgtgatgttg ttatcggttg
    42061 gactgctttt ggcgggctgt tcgggtagca aatcatccga tacaggaacg tattccggct
    42121 ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc acgggaacca
    42181 gcgtaaaaga actggcgcga ctgaacggca tttccccccc ttacaccatt gaagttggtc
    42241 agaaactaaa actgggtggg gcgaaaagta gcagtagtac acgtaaatca accgccaaat
    42301 caacgaccaa aaccgcatcg gttacaccgt catcagcggt accgaaatca tcctggccgc
    42361 cagtagggca acgttgttgg ttatggccaa cgacagggaa agttatcatg ccgtattcga
    42421 cagcagatgg cggcaataaa gggattgata tctcagctcc acggggtaca cctatttacg
    42481 ccgcgggtgc aggaaaggtg gtgtatgtgg gcaaccagct gcgtggctac ggtaatctca
    42541 tcatgattaa acacagtgaa gattacatta cggcttacgc ccataatgac acgatgctgg
    42601 taaataatgg gcaaagcgtg aaggctgggc aaaaaatcgc cactatgggg agcacggatg
    42661 cggcatctgt tcgcctgcat ttccagattc gttaccgtgc aacggcaatt gatccgctac
    42721 gttacttgcc gccgcagggc agcaagccaa aatgctgatg gcgaattaat cagcagtcag
    42781 cagcgcggca cttgctaagg agagcgtaag gtttataatg ccttacgcat ctcgaagcgg
    42841 gcgtagttca atggtagaac gagagcttcc caagctctat acgagggttc gattcccttc
    42901 gccgcttttg ctgtatctga ttttctatat tatcttaatt caatcgcttc agacttttcg
    42961 caatcgccgt aatatttccc cattttgttg taaataaaaa gcgtttacgc aatgcaatcc
    43021 ttttgtaaca acccaggtaa aacaccttat tttcaagttg ttaaataagt ggttatttac
    43081 gcttttaaca aacgattgat ggaatatttt atgaaaggga aatctgcatt aactcttctg
    43141 ctcgctggta tattttcgtg cggcacatgc caggcgacag gggcagaagt gacgagtgaa
    43201 tctgtattta acattcttaa ttccacgggg gcagcaacag ataaaagtta tttatcgtta
    43261 aatccagata agtaccctaa ttatcgtctt ctgattcact cagccaaatt aaaaaatgaa
    43321 ataaagagcc actataccaa agacgaaatt caagggttgt taacattaac agaaaacaca
    43381 cgaaagttaa cgttgacgga gaaaccctgg ggaacattta ttcttgcttc tactttcgaa
    43441 gatgacaaaa cagcggcaga aacccactat gatgctgtct ggctgaggga tagtttatgg
    43501 ggctatatgg cattagtatc agatcaggga aatagtgtag ccgcaaaaaa ggttctgctc
    43561 accctgtggg attatatgtc tacgctcgac cagattaagc ggatgcagga cgtcattagc
    43621 aaccccaaac gactggatgg agttcccggg caaatgaatg ctgtgcatat ccgatttgac
    43681 agcaactccc ccgtgatggc agatgtgcag gaagaaggca agccgcagct gtggaaccat
    43741 aaacaaaatg atgcgttagg tctttatctt gatctcctta tacaagcaat cgacactggt
    43801 acaattaacg ctgaagattg gcaaaaaggt gaccgattaa aatcggttgc gctgctgatt
    43861 gcttatctgg ataaagccaa tttctatgtt atggaagatt ccggagcatg ggaagaagat
    43921 gcccgattaa acacatcttc cgttgcactg gtgacctctg gactggaacg tttgtcgaac
    43981 ttactctcga agaaagactc tgtttttgtc tctgatttac tacgcgaagc taaagccaat
    44041 gaactggatg aacctctttc aacaacgagg ttgaaccacc tcatagataa aggttatgaa
    44101 agaataacgt tacagcttga tttaggggga gaatccccag gttatctgga aaaagataaa
    44161 cattacagag aagccgatgc ggctttactt aatgttattt atcctgcaaa tcttgcaaag
    44221 ataaacacca gaagaaaaga gcaggttcta aaaatagtta aaaaactagc aggtccgtat
    44281 ggtataaaaa gatacgagaa ggataattat cagtcggcaa atttttggtt caatgatatt
    44341 aaaaccgata ccgatcaaaa ttcacatgcg aaaag
//
LOCUS       DAZOZY010000010        44203 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZOZY010000010 DAZOZY010000000
VERSION     DAZOZY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44203
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.010"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..559)
                     /locus_tag="ACHURQ_000502"
     CDS             complement(<1..559)
                     /locus_tag="ACHURQ_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR0822372.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHURQ_000503"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="ACHURQ_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR0822373.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHURQ_000504"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="ACHURQ_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR0822374.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHURQ_000505"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="ACHURQ_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR0822375.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="ACHURQ_000506"
     CDS             complement(4001..6322)
                     /locus_tag="ACHURQ_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR0822376.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHURQ_000507"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="ACHURQ_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR0822377.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="ACHURQ_000508"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="ACHURQ_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR0822378.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="ACHURQ_000509"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="ACHURQ_000509"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR0822379.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHURQ_000510"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="ACHURQ_000510"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR0822380.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..12968
                     /locus_tag="ACHURQ_000511"
                     /pseudo
     CDS             11268..12968
                     /locus_tag="ACHURQ_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHURQ_000512"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="ACHURQ_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR0822381.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHURQ_000513"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="ACHURQ_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR0822382.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHURQ_000514"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="ACHURQ_000514"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR0822383.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHURQ_000515"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="ACHURQ_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR0822384.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="ACHURQ_000516"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="ACHURQ_000516"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR0822385.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHURQ_000517"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="ACHURQ_000517"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR0822386.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="ACHURQ_000518"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="ACHURQ_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR0822387.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="ACHURQ_000519"
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="ACHURQ_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR0822388.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="ACHURQ_000520"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="ACHURQ_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR0822389.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="ACHURQ_000521"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="ACHURQ_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR0822390.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHURQ_000522"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="ACHURQ_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR0822391.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="ACHURQ_000523"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="ACHURQ_000523"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR0822392.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="ACHURQ_000524"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="ACHURQ_000524"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR0822393.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="ACHURQ_000525"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="ACHURQ_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR0822394.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="ACHURQ_000526"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="ACHURQ_000526"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0822395.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="ACHURQ_000527"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="ACHURQ_000527"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR0822396.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="ACHURQ_000528"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="ACHURQ_000528"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR0822397.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="ACHURQ_000529"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="ACHURQ_000529"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR0822398.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHURQ_000530"
     CDS             31280..32185
                     /gene="yhfZ"
                     /locus_tag="ACHURQ_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR0822399.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="ACHURQ_000531"
     CDS             32202..32564
                     /locus_tag="ACHURQ_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR0822400.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="ACHURQ_000532"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="ACHURQ_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="ACHURQ_000533"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="ACHURQ_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="ACHURQ_000534"
     CDS             35034..35912
                     /locus_tag="ACHURQ_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR0822401.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="ACHURQ_000535"
     CDS             35923..36276
                     /locus_tag="ACHURQ_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR0822402.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="ACHURQ_000536"
     CDS             36288..37592
                     /locus_tag="ACHURQ_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR0822403.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="ACHURQ_000537"
     CDS             37604..38689
                     /locus_tag="ACHURQ_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR0822404.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHURQ_000538"
                     /gene_synonym="yhfR"
     CDS             complement(38841..39572)
                     /gene="frlR"
                     /locus_tag="ACHURQ_000538"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR0822405.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHURQ_000539"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="ACHURQ_000539"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR0822406.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHURQ_000540"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="ACHURQ_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHURQ_000541"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="ACHURQ_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR0822407.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHURQ_000542"
     CDS             complement(42378..43715)
                     /gene="frlA"
                     /locus_tag="ACHURQ_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR0822408.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            complement(44010..44177)
                     /locus_tag="ACHURQ_000543"
     CDS             complement(44010..44177)
                     /locus_tag="ACHURQ_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR0822409.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT        10312 a        11212 c        12100 g        10579 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctagttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac aattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctggctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatag ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctcc tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaggc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggt gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaacccg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt cccatagcga tacctttttc ttgtccaata
    43741 cgttgtaggg gagagtttgc agtcatttaa aaggtaacat gacaatgcat gatgaatata
    43801 acaacatatg atgttatgta atgtggagga agatcacaaa atttcgcaca ggatcgcgct
    43861 gtggctaatg gatgtagtta tcaaattgaa tttaaagtga aaatattttt acgggcgggg
    43921 gcaagaagga catagaaaca aatacgccct cggaaaatcc agagggcgtc gggcaattaa
    43981 accggtgtta gccgatttct gtcagagact tactgtgcag taggaccgca accgccaatg
    44041 attttagaaa tagaaattgc cgggtgcagc aggtagtcgt aagagcagtt cttatcacgg
    44101 ttttcaatat gaccagtaca tgcagttaac gtagccagta ctgcacctac cagtgcaact
    44161 ttaataaatt tgttcattta cattccctat gtattatata tta
//
LOCUS       DAZOZY010000011        43810 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZOZY010000011 DAZOZY010000000
VERSION     DAZOZY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.011"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..71)
                     /locus_tag="ACHURQ_000544"
     CDS             complement(<1..71)
                     /locus_tag="ACHURQ_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822410.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLC"
     gene            89..280
                     /locus_tag="ACHURQ_000545"
     CDS             89..280
                     /locus_tag="ACHURQ_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR0822411.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            591..1109
                     /locus_tag="ACHURQ_000546"
     CDS             591..1109
                     /locus_tag="ACHURQ_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR0822412.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            1125..1664
                     /locus_tag="ACHURQ_000547"
     CDS             1125..1664
                     /locus_tag="ACHURQ_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR0822413.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1759..3336)
                     /gene="guaA"
                     /locus_tag="ACHURQ_000548"
     CDS             complement(1759..3336)
                     /gene="guaA"
                     /locus_tag="ACHURQ_000548"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR0822414.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(3405..4871)
                     /gene="guaB"
                     /locus_tag="ACHURQ_000549"
     CDS             complement(3405..4871)
                     /gene="guaB"
                     /locus_tag="ACHURQ_000549"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR0822415.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            5033..6403
                     /gene="xseA"
                     /locus_tag="ACHURQ_000550"
     CDS             5033..6403
                     /gene="xseA"
                     /locus_tag="ACHURQ_000550"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR0822416.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(6400..6615)
                     /locus_tag="ACHURQ_000551"
     CDS             complement(6400..6615)
                     /locus_tag="ACHURQ_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR0822417.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6684..8156)
                     /gene="der"
                     /locus_tag="ACHURQ_000552"
     CDS             complement(6684..8156)
                     /gene="der"
                     /locus_tag="ACHURQ_000552"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR0822418.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(8274..9452)
                     /gene="bamB"
                     /locus_tag="ACHURQ_000553"
     CDS             complement(8274..9452)
                     /gene="bamB"
                     /locus_tag="ACHURQ_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR0822419.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(9463..10083)
                     /gene="yfgM"
                     /locus_tag="ACHURQ_000554"
     CDS             complement(9463..10083)
                     /gene="yfgM"
                     /locus_tag="ACHURQ_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR0822420.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(10101..11375)
                     /gene="hisS"
                     /locus_tag="ACHURQ_000555"
     CDS             complement(10101..11375)
                     /gene="hisS"
                     /locus_tag="ACHURQ_000555"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR0822421.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(11486..12604)
                     /gene="ispG"
                     /locus_tag="ACHURQ_000556"
                     /gene_synonym="gcpE"
     CDS             complement(11486..12604)
                     /gene="ispG"
                     /locus_tag="ACHURQ_000556"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR0822422.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12631..13644)
                     /gene="rodZ"
                     /locus_tag="ACHURQ_000557"
     CDS             complement(12631..13644)
                     /gene="rodZ"
                     /locus_tag="ACHURQ_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR0822423.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13929..15083)
                     /locus_tag="ACHURQ_000558"
     CDS             complement(13929..15083)
                     /locus_tag="ACHURQ_000558"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR0822424.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(15233..15664)
                     /gene="ndk"
                     /locus_tag="ACHURQ_000559"
     CDS             complement(15233..15664)
                     /gene="ndk"
                     /locus_tag="ACHURQ_000559"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR0822425.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15813..18125)
                     /gene="pbpC"
                     /locus_tag="ACHURQ_000560"
     CDS             complement(15813..18125)
                     /gene="pbpC"
                     /locus_tag="ACHURQ_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR0822426.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(18126..23087)
                     /gene="yfhM"
                     /locus_tag="ACHURQ_000561"
     CDS             complement(18126..23087)
                     /gene="yfhM"
                     /locus_tag="ACHURQ_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR0822427.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            23294..24139
                     /gene="sseA"
                     /locus_tag="ACHURQ_000562"
     CDS             23294..24139
                     /gene="sseA"
                     /locus_tag="ACHURQ_000562"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR0822428.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            24475..24600
                     /gene="timP"
                     /locus_tag="ACHURQ_000563"
     CDS             24475..24600
                     /gene="timP"
                     /locus_tag="ACHURQ_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR0822429.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24632..25408)
                     /gene="sseB"
                     /locus_tag="ACHURQ_000564"
     CDS             complement(24632..25408)
                     /gene="sseB"
                     /locus_tag="ACHURQ_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR0822430.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25550..26833)
                     /gene="pepB"
                     /locus_tag="ACHURQ_000565"
     CDS             complement(25550..26833)
                     /gene="pepB"
                     /locus_tag="ACHURQ_000565"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR0822431.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(27011..27211)
                     /gene="iscX"
                     /locus_tag="ACHURQ_000566"
     CDS             complement(27011..27211)
                     /gene="iscX"
                     /locus_tag="ACHURQ_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR0822432.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(27223..27558)
                     /gene="fdx"
                     /locus_tag="ACHURQ_000567"
     CDS             complement(27223..27558)
                     /gene="fdx"
                     /locus_tag="ACHURQ_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR0822433.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27560..29410)
                     /gene="hscA"
                     /locus_tag="ACHURQ_000568"
     CDS             complement(27560..29410)
                     /gene="hscA"
                     /locus_tag="ACHURQ_000568"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR0822434.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29427..29942)
                     /gene="hscB"
                     /locus_tag="ACHURQ_000569"
     CDS             complement(29427..29942)
                     /gene="hscB"
                     /locus_tag="ACHURQ_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR0822435.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(30038..30361)
                     /gene="iscA"
                     /locus_tag="ACHURQ_000570"
     CDS             complement(30038..30361)
                     /gene="iscA"
                     /locus_tag="ACHURQ_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR0822436.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30378..30764)
                     /gene="iscU"
                     /locus_tag="ACHURQ_000571"
     CDS             complement(30378..30764)
                     /gene="iscU"
                     /locus_tag="ACHURQ_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR0822437.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30792..32006)
                     /gene="iscS"
                     /locus_tag="ACHURQ_000572"
     CDS             complement(30792..32006)
                     /gene="iscS"
                     /locus_tag="ACHURQ_000572"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR0822438.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(32247..32735)
                     /gene="iscR"
                     /locus_tag="ACHURQ_000573"
     CDS             complement(32247..32735)
                     /gene="iscR"
                     /locus_tag="ACHURQ_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR0822439.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(33036..33773)
                     /gene="trmJ"
                     /locus_tag="ACHURQ_000574"
     CDS             complement(33036..33773)
                     /gene="trmJ"
                     /locus_tag="ACHURQ_000574"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR0822440.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33892..34695
                     /gene="suhB"
                     /locus_tag="ACHURQ_000575"
     CDS             33892..34695
                     /gene="suhB"
                     /locus_tag="ACHURQ_000575"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR0822441.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34840..35694
                     /locus_tag="ACHURQ_000576"
     CDS             34840..35694
                     /locus_tag="ACHURQ_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0822442.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35885..37165
                     /gene="csiE"
                     /locus_tag="ACHURQ_000577"
     CDS             35885..37165
                     /gene="csiE"
                     /locus_tag="ACHURQ_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR0822443.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(37157..38296)
                     /locus_tag="ACHURQ_000578"
     CDS             complement(37157..38296)
                     /locus_tag="ACHURQ_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR0822444.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38456..39346)
                     /gene="hcaR"
                     /locus_tag="ACHURQ_000579"
     CDS             complement(38456..39346)
                     /gene="hcaR"
                     /locus_tag="ACHURQ_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR0822445.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            39482..40843
                     /gene="hcaE"
                     /locus_tag="ACHURQ_000580"
     CDS             39482..40843
                     /gene="hcaE"
                     /locus_tag="ACHURQ_000580"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR0822446.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40840..41358
                     /gene="hcaF"
                     /locus_tag="ACHURQ_000581"
     CDS             40840..41358
                     /gene="hcaF"
                     /locus_tag="ACHURQ_000581"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR0822447.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            41358..41678
                     /locus_tag="ACHURQ_000582"
     CDS             41358..41678
                     /locus_tag="ACHURQ_000582"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR0822448.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41675..42487
                     /gene="hcaB"
                     /locus_tag="ACHURQ_000583"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41675..42487
                     /gene="hcaB"
                     /locus_tag="ACHURQ_000583"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR0822449.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42497..43699
                     /gene="hcaD"
                     /locus_tag="ACHURQ_000584"
     CDS             42497..43699
                     /gene="hcaD"
                     /locus_tag="ACHURQ_000584"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR0822450.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10408 a        12072 c        10955 g        10375 t
ORIGIN      
        1 ttacataacg aagaattgac cattttgtcc tgttgtgcct taatgtaagt accgtccaca
       61 gcgtgggaca tacttcaagg aaccttttgt gagtcaggca accagtatgc gaaaacgaca
      121 ccgatttaac agtcgcatga cccgtatcgt actgctcatc agctttatct tcttctttgg
      181 ccgttttatc tactcgtccg tcggtgcctg gcagcaccat cagagcaaaa aagaagctca
      241 gcaatccaca ctctccgtcg aatcaccggt acaacgttag cggttacctt cttcactttc
      301 acagaacata acggcacttc gctgtcggat gcttttgctc tttgggatta tcaaagcggc
      361 agatattctt tcatcttaaa ttttacgtct ttgtcctgac tgatgtttat cctgtttggc
      421 tgcggaataa atataaaatt aatatatatg ttgtaatgat atatttttat aaattattcc
      481 ctgcgtgaat tttaataaat ttaatctatc cctttatacg caatacattt actttcctct
      541 tttgatgatc ttaaatgtct tatttttcgt aatgtgtata acaaggaata gtgatgaaat
      601 ttaaaaaatg tcttctgcct gtggcaatgt tagcgtcatt cactctggca ggatgccagt
      661 caaatgctga cgatcatgcc gccgatgttt atcaaaccga tcaactgaat accaaacaag
      721 aaactaaaac cgttaatatt atttccattc ttcccgcaaa agttgccgta gacaactccc
      781 aaaataaacg gaacgcacaa gccttcggcg cgcttattgg cgcagtcgct ggcggtgtta
      841 tcggccacaa cgtcggttct ggcagcaatt ccggaacgac ggcaggggca gttggcggcg
      901 gagctgtagg cgcggcagcg ggttctatgg tgaatgataa aaccttagtg gaaggtgttt
      961 ctttaacata taaggaaggc accaaagtgt atacctccac ccaggtgggt aaagagtgcc
     1021 agtttacgac aggtttagcc gttgttatta ccacaacgta taacgaaacg cgtattcagc
     1081 caaatactaa atgtcctgaa aagagctaat aatcaggagg agtcatgaag aaagtttttc
     1141 tttgcgccat cttagcctcc ttaagctatc cggctatcgc ctcatcattg caggatcaac
     1201 tctctgctgt cgcagaagcg gaacagcaag gtaaaaatga agagcaaagg cagcatgacg
     1261 aatgggtcgc ggagcgcaac agggaaatcc agcaagagaa gcaacgtcgc gcaaatgccc
     1321 aggccgccgc taacaaaaga gcggcaacgg cagcggcaaa taagaaagct cgtcaggata
     1381 aactggacgc cgaagccact gcggacaaaa aacgcgatca aagttatgaa gatgagctac
     1441 gcagcttaga gattcagaaa caaaaactga cgctggcgaa agaagaagcc cgcgttaagc
     1501 gagaaaacga atttatcgat caggaactga agcacaaagc tgcgcaaacc gatgtggtgc
     1561 aatctgaagc tgacgccaac agaaatatga ctgaaggcgg tcgcgatctg atgaaaagcg
     1621 tgggcaaagc agaagagaac aaatcggaca gctggtttaa ttaatcgatg ttagtaactt
     1681 caagcctatg attcttgaag ataaaaaacc ctctgtagta acagagggtt ttgttcattc
     1741 atagtgcagg gattaaaatc attcccactc aatggtagct ggcggcttgc cgctgatgtc
     1801 atacaccacg cgggaaatac cgttcacttc attgataatg cggttggaaa cgcgaccgag
     1861 gaaatcgtac ggcagacgcg cccagtgtgc ggtcataaag tcgatggttt cgacagcacg
     1921 cagagagaca acccagtcat acttacgacc atcgcccatt acgccaacgg aacgtaccgg
     1981 caggaacaca gtgaacgcct ggctgacttt gtcgtacagg tccgctttac gcagttcttc
     2041 aatgaagatg gcgtcagcac ggcgcagcag gtcacagtac tctttcttca cttcacccag
     2101 cacacgaacg ccaaggcctg gtcccgggaa tgggtgacgg tacaacatgt cgtacggcag
     2161 gcccagctcc agaccaatct tacgcacttc gtctttgaac agctctttca gcggttcaac
     2221 caggcccatc ttcatctctt tcggcaggcc gcccacgttg tggtgagatt tgatgacgtg
     2281 tgctttaccg gttgcagacg ccgcagattc gataacgtca gggtagatgg tgccctgcgc
     2341 cagccacttc acgtcttcca gtttcagcgc ttcttcatcg aatacttcaa cgaaaacgcg
     2401 accgatgatt ttacgttttg cttccggatc gttttcgcca gccagcgctg acaggaagcg
     2461 atcttctgcc ggaacgtgaa caatgttcag accaaagtga tcgccaaaca tatccagaac
     2521 ctgctctgct tcgttgagac gcagcaggcc gttgtcgacg aatacgcaag tcaggttttt
     2581 accgatagcg cggtgcagta gcattgcggt tacggaggaa tccacgccac cagagaggcc
     2641 gaggatgact ttatcgtcgc ctacctgctc acggatgcgg gctacagcat cgtcgataat
     2701 tttcgctggc gtccacaggg cttcacactg gcagatatca cgcacaaaac gctccagcat
     2761 gcgcataccc tggcgggtgt gagtcacttc cgggtggaac tgtacgccat agaagcgttt
     2821 ttcttcgtta gccataatgg caaacgggca gctttcggtg ctggctacgg tgacgaagtc
     2881 ggacgggata gcggtaactt tatcgccgtg gctcatccag acatcgagca gcggtttacc
     2941 gtctgcggtc agcgcatctt cgataccgcg aaccagtgca ctgtcgttta cgacttcaac
     3001 ctgcgcgtag ccaaattcac gttcgttaga ggcttcaacg tgaccgccca actgcattgc
     3061 catggtctgc atgccatagc aaacgccgaa taccggtacg cctgcttcaa agacatactg
     3121 cggcgcacgc gggctgtttt cttcagtagt actttccggg ccgccggaaa gaataatgcc
     3181 gcttggattg aagtcacgaa tttgtgcttc tgtcacatcc cacgcccaca gttcgcagta
     3241 aacacccagc tcacgcacgc ggcgcgcaac cagttgagtg tactgagaac cgaagtccag
     3301 aatgaggatg cgatgcttat gaatgttttc cgtcattgac gcttattccg aggcaagtga
     3361 aacagataat ataaatcgcc cgacatgaag tcgggcgaag agaatcagga gcccagacgg
     3421 tagttcgggg actctttagt aatggtcacg tcgtgaacgt ggctttcctg aatgcccgca
     3481 ccgctgatac gtacaaactc cgctttagta cgcagttcgt cgatagtacc acagccggtc
     3541 agacccatac aggagcgcag gccgcccatc tgctggtgaa tgatctcttt caggcgacct
     3601 ttataagcta cgcgaccttc gataccttcc ggcaccagtt tgtcggcagc gttatcgctc
     3661 tggaaataac ggtcagagga acctttggac atcgcgccca gggaacccat accacggtaa
     3721 gatttgtaag aacggccctg gtagagttcg atttcacccg gagattcttc agtacccgcc
     3781 agcatggaac ctaccatcac cgcgcttgcg ccagcggcga tagctttggc gatgtcgccg
     3841 gagaagcgaa taccaccatc tgcgataacc ggaataccgg tgccttccag ggcttcaact
     3901 gcgtcagcaa cggcagtgat ctgcggaaca cctacgccag taacgatacg agtcgtacag
     3961 atagaaccag ggccgatacc gactttaacc gcgctgcaac cagcttctgc caaagcgcgt
     4021 gcacctgcag ctgttgccac gttgccgccg atgatttgca gatccggata tttagcacgg
     4081 gtttcacgga tacgctgcaa aacgccttct gagtgaccgt gggaggagtc gatcagcaga
     4141 acgtcaacgc cagcggcaac cagcgcgtca acacgctctt cgttacccgc acctgcgcca
     4201 actgcagcac caacacgcag acggccttgc tcgtctttac aggcgttcgg tttacgttcc
     4261 gctttctgga agtctttcac ggtgatcatg ccgatcaggt ggaattcgtc atcaaccacc
     4321 agcgcttttt caacgcgttt ttcgtgcatt tttgccagca ccacttcacg ggcttcacct
     4381 tcacgcacgg tgaccagacg ctctttcggc gtcatgtaaa cgctaaccgg ctggttcagg
     4441 tcggtaacaa aacgcacgtc acgaccggtg ataataccca ccagttcgtt ttcttcggta
     4501 acgaccggat agcccgcaaa accgttacgc tcggtcagtt ctttcacttc gcgcagcgtc
     4561 gtggttggca gaacagtctg cggatcagtc accacaccag attcgtgttt tttcacacgg
     4621 cgaacttctt ctgcctggcg ttcaatggac atgtttttgt ggataaagcc gataccgcct
     4681 tcctgagcca gagcaatagc caggcgcgct tccgttacgg tatccattgc tgcggaaagc
     4741 atagggatat tcagacgaat agttttcgtc agctgggtgc tgaggtcagc agtattcggc
     4801 agaacggtag aatgagcagg aacgaggaga acgtcgtcaa acgtcagagc ttctttagcg
     4861 atacgtagca tgggcaatat ctcgaccaga gtggttaata aatattgccg cggcattata
     4921 cagagcgtaa ccgattgcat ctaccccttt ttgcaaaaaa tgcttgctat ccccgaaggg
     4981 cgggttacta tcgactgaat aacctgctga tttagaattt gatctcgctc acatgttacc
     5041 ttctcaatcc cctgcaattt ttaccgttag tcgcctgaat caaacggttc gtctgctgct
     5101 tgagcatgag atgggacagg tttggatcag cggcgaaatc tctaatttca cacaaccggc
     5161 ttccggtcac tggtacttta cactcaaaga cgacaccgcc caggtacgct gcgcgatgtt
     5221 ccgcaacagc aaccgccggg tgaccttccg tccacagcat ggacaacaag ttttagttcg
     5281 cgccaatatt acgctctacg agccgcgcgg tgactaccag ataatcgttg agagtatgca
     5341 gccggccggt gaagggctgc tgcaacagaa gtacgaacag ctcaaagcga agttgcaggc
     5401 tgaaggtttg ttcgatcagc aatacaaaaa accacttccc tcccctgcgc attgcgttgg
     5461 tgtgatcacc tcaaaaaccg gtgctgcgct acatgatatt ttgcatgtgt taaaacgtcg
     5521 cgatccttct ctaccggtga tcatctaccc caccgccgtt cagggcgatg acgcaccggg
     5581 gcaaattgtt cgcgccattg aactggcgaa tcagcgcaat gagtgcgacg tgttgatcgt
     5641 tgggcgcggc ggcggttcgc tggaagattt atggagtttt aacgacgaac gcgtagcgcg
     5701 ggcgattttt gccagccgca ttccggtcgt cagcgccgtc gggcatgaga cggatgtgac
     5761 cattgccgat tttgttgccg atctgcgtgc gccaacgccg tctgccgccg ctgaagtagt
     5821 gagccgtaat cagcaagagt tactgcgcca ggtgcaatcg acccgtcaac ggctggagat
     5881 ggcgatggat tattatctcg ccaaccgcac gcgtcgcttt acgcagatcc atcaccgatt
     5941 acagcaacag catccgcagc tccggctggc acgccagcaa accatgcttg agcgcctgca
     6001 aaagcggatg agctttgcgc tggaaaatca acttaagcgt accgggcaac agcagcagcg
     6061 gttaacacag cggctgaatc agcaaaatcc acagccgaag attcatcgcg cgcaaacgcg
     6121 cattcagcaa ctggaatatc gtttagcaga aaccctgcgc gtacagctta gcgccacgcg
     6181 tgaacgtttc ggtaatgcag taacgcacct cgaagccgta agcccactgt caacgctggc
     6241 gcgtggatac agcgttacta ctgctactga cggcaatgta ctgaaaaaag tgaagcaagt
     6301 taaagcgggt gaaatgctaa ccacacgtct ggaagacggc tggatagaaa gtgaagttaa
     6361 aaacatccag ccagtaaaaa aatcgcgtaa aaaggtgcat taagccagca cgaattcaac
     6421 gcgttttttc gatatcaagc catgcccgtg ctgacagaag taatctactg caccgcaggc
     6481 ttttaacacc tgcaacggtt gatggcagtc tggacaaaga gctttcattt caataatttc
     6541 cccgcaggat gggcaacgcg catggccgtt atcctgatca agcacatgct gacattgtgg
     6601 acaatgcagt tccatatgaa ttcctccggt agccatccct ctacattcat agagggaatg
     6661 gcagataaaa tacttacgga taattattta tttttcttga tgtgcttcat cagacgctta
     6721 cgtttacgca tctgggttgg cgtcagggtg ttacgcttgt tcgcatacgg gttttcccct
     6781 tctttgaact gaatacgaat cggcgatccc attacgtcca gcgatttgcg gaagtagttc
     6841 atcaagtagc gcttgtagga atcaggcagg tctttcacct gattaccgtg aatcaccaca
     6901 atcggcgggt tataaccacc agcgtgggca tatttcagct tcacacgacg accgcgtacc
     6961 agcggcggtt ggtgatcttc aacagccatc gtcatgatgc gcgtcagcat agaggtcccc
     7021 acacgacggg tggagctgtc atacgcttca cgtactgatt caaacaagtt accaacacca
     7081 ctgccgtgca aggcagagat aaagtgcaca cgagcaaaat cgataaagcc cagacggaag
     7141 tccagcgttt ctttcacctg ctctttcact tcctggctca ggccatccca cttattcacc
     7201 acaatgacaa gtgagcgccc actattgaga ataaagccca gcagcgagag atcctgatcg
     7261 gaaatacctt cgcgcgcatc aatcactaac atcaccacgt tggtatcttc aatggcctgc
     7321 aacgttttga ttacggagaa tttctctaca gcatcggtga ttttgccgcg tttacgtacg
     7381 ccagcggtgt caatgagcac atactcacgt ccatcgcgtt ccattggaat gtagatactg
     7441 tcacgcgtcg tgccaggcat gtcataaaca acaacgcgct cttcaccaag aatacggtta
     7501 gtgagtgtag acttacctac gttcggacga cccacaatcg ccagtttgat cggcagactt
     7561 tgcgggtcga agtcgtcttc ctcttcttct tcgccgttct cttccgcttc aaattgcgcc
     7621 cagtattcag cgtcttcgtc gacttcctct tgcggtgcga gatcttccat ccacggcagc
     7681 agcacacgct ccagcagact taatacgcca cgaccgtgag atgcggcgat cgggtagatt
     7741 tcacctaaac caagcgagta gaaatcaacc actgcctgat cgggatccag accgtcagtt
     7801 ttgtttgcca ccaggaaggt cggtttttca cgagagcgca gatgtttggc aatcgcttca
     7861 tctgccggca tcaagcccgc gcgcgcatcc accataaaca gtacgacgtc cgcttcttca
     7921 atcgccagca gcgactgttc cgccatgcgg gtttctacac cgtcttctgt gccatcaatc
     7981 ccgccggtat caatacagat aaactcacgg ccttcaattt ccgcacgacc gtacttacgg
     8041 tcacgagtca gacccgggaa atccgcaacc agcgcatctc gggtgcgagt tagacggtta
     8101 aataacgtgg attttcctac gttagggcgc ccgacaagcg cgaccacagg taccatgttt
     8161 aaagcctcat ttttataaat catcagacaa cgcacgctat attcgcgtcg ttgttaaaaa
     8221 caggaaaacg gcccctgtcc aggagccgtt ttcaaagtga acgacagaga cgattaacgt
     8281 gtaatagagt acacggttcc gtcttttgcc tggatcagca gtttgccgtc agcggcaacc
     8341 ggttcagtct ggaaaccgga actatcaact ttttgctggg caacgaaacg accatcttcg
     8401 acgttaatcc agtgcagata accttcactg tcacctacca ccaggttgcc attatacagc
     8461 accggcgaag tcagcaggcg atgcagcaga tcgctttgtg tccacagcgt aacgccgcca
     8521 tcaatggtca acgccatcac ccggtcattt tgatcgacca gatagatgcg attgccgtcg
     8581 acgatgaaat cattcaccga acccagttcg cgtttccaca taatctgacc actgcgcaga
     8641 tcaagcgccg tcaggttacc attataggcc agagcgaaaa caacgccgtt aacaacgacg
     8701 ggagtcgtgt caacatcgct cagacggtca atttcggtag aaccggtcgc ctgggaaata
     8761 cgctgctgcc aaatcatctg gccctgttcc atcagcactg cgctgacgcg accattatcg
     8821 ccccccacga cggccgcacc aaaagccgtt gccggcgcag actcgccacg caaagagagc
     8881 gaaggcatat cgaggttaac tgtccatttg acagcgccgt cagcttcgtt cagcgcttgt
     8941 aactgaccgt tactagtgtg gattaacacc agaccgtcgc tgaccaccgg gcgagaaagt
     9001 gcttcacccg cgactttagt ttgccatgcc acagtaccat cgctggtatt cagcgcgtaa
     9061 acctgcgcct tttcgctgcc aatgtagaca tgcccaccag acacggtcac accgccagaa
     9121 agtaatgcag gctctttaga gaaccagcca tctttctcgg ccaggctgac agaccagatt
     9181 tctttgccat catccgcatt cagcgctttt accaaaccag cgcggtccgc tgcatagaca
     9241 acgttgtccg ccagtgccgg atgaagattg gaatagaagt tgccaatgcc gctaccaacg
     9301 gaagtgctcc acgccgtggt cggcgtaaac tggttttcaa cggttggcaa tggggacatc
     9361 tttaccacat cttcttcgct gttaaacagc gaacagccgc ttaaaagggt aacggaaagc
     9421 agtcctggca gcagtaattt acgcaattgc atcgggtccc tctcagatgg acaaattatt
     9481 aattttcatc tgcatcattt cgctcagtgc cggagtaaca tcgcttttca cgcctgcttc
     9541 ccatgcacta cgcgcacctt gcttatcacc tttgctcaac aatgctttac cacgcaggtc
     9601 ggcaacaatg gcagcccacc cttcaccttt gatggtatca agggttttca gcgcggcatc
     9661 agcctgcttg agctgtacct gaacgcgagc aagacgcaga tttatcacgg ctttgagatt
     9721 ttcatcactc gtgtctgcca gcccctgttg taactgggcg gcagctttct ctagttcatt
     9781 tttgtcaaca aattgctgcg caagttccaa agaagccagc gcaccataag tatttttatt
     9841 ttcagcagca aatttttccg ccgccgggat gctatccggt ttgccttcgc tcactgcggt
     9901 aaccgcattt tgataggcaa gagaagcgga gcgtgcagaa tcaacctgat ggctgttcca
     9961 gtagcgccag ccaatcagtg cgccaacgcc caaaatcacc ccaacagcca gtgctttgcc
    10021 attttcagca aaaaagcgtt taaccgcttc tacctggtcg ttttcgttct cgtaaatttc
    10081 cacgctgtcc ttctccttcc ttaacccagt aacgtgcgca aatgcgcggc tacgctatcc
    10141 tgcgcaactg ccgtttgctc accagagcgc aaatccttca ctactgctgt gccgttagcc
    10201 acttcagact cacccagcac cacagcaacg cgggcacccc atttatcagc acgggcaaac
    10261 tgtttcttaa agttgccgcc gccgtggttg gtcatcaatt tcacgcccgg taattcatca
    10321 cgcagacgct cagctaatgc catagccgca gattgtgtat cagcacctga agccaccagg
    10381 tatatatcga caacaggatc ggctttaaat tccggattaa cggcctgtac taacaataca
    10441 agacgttcga ggcccatcgc aaaaccgact gccggtgttg cacgaccgcc cagttgttcc
    10501 accagaccgt cataacgacc gcctgcacac acggtgccct gggagccgag gctgttagtc
    10561 acccactcga aaacggtacg gttatagtaa tccagaccac gcaccagacg ctggtttacg
    10621 gtgtaagcga tccccgcgct ctcaagcagt ttgcacagac cggcaaaatg ctcacgagat
    10681 tcctcgtcca gataatcacc taatgccgga gcgtcgttga gaagcgcctg cacttccgga
    10741 ttttttgaat ccagcacgcg cagcgggtta gtgtacatgc ggcgtttgca gtcttcgtcc
    10801 agcttttctt tatgctgctc aaggaatgcc accagcgcat cgcggtaatt ggcgcgtgct
    10861 tccagcgaac caatagagtt cagctcaaga gttacgtgtt cgaagatacc cagcgcacgc
    10921 caccagcggg cagtgagcat aatcagttca gcgtcgatat ccggaccttg cagaccgaaa
    10981 acttcgcagc ccaactgatg gaactgacga taacgccctt tctgcggacg ctcgtgacgg
    11041 aacatcggcc cgatatacca cagacgctgt tcctgattgt acagaagacc atgctcgatg
    11101 ccggcgcgta cacagcccgc cgtcccttca gggcgcagag tcaggctgtc gccattgcga
    11161 tcctcaaagg tgtacatctc tttttcaacc acgtcggtga cttcaccaat cgcacgtttg
    11221 aatagcgggg tctgctctac aatcggcaag cggatttcac tgtaaccgta gctgccgagc
    11281 acgtttttca gtgtgccttc aatgcgctgc cagatggccg tttcgccagg caggtaatcg
    11341 ttcatgccgc gaatggcttg aatgtttttt gccacgttta ttctctttct gaatataaaa
    11401 atgaaccctc aacgcttccc tcaatgtttc gggagccatg cgggttcaat catacacggg
    11461 aagcgaggcg cttcccatca cgttattatt tttcaacctg ctgaacgtca attcgacgcg
    11521 cttcgtccag ctgactggct ttcgcacgaa tgcgtgcttc cagctggtcg atcatatcgt
    11581 tgttgtccag acggtctttg cgcacgccat cttcatagag gccgcttttc ttgttgccgc
    11641 cggtgacgcc gagtgtagaa accagcgcct cacctgggcc attcaccacg cagccgataa
    11701 tcgaaacgtc catcggagtg atgatatctt ccaggcgttg ctccagcgcg ttaaccgtac
    11761 cgataacatc aaattcctga cgcgaacagg tcgggcaggc gatgaagtta atccctcgcg
    11821 aacggatacg cagcgatttc aaaatatcga aaccgacttt gatctcttcg accggatcgg
    11881 ccgccagcga tacgcgcagc gtgtcgccga tgccttccga cagcagcaga cctaaaccaa
    11941 tggcggattt tactgccccg ctgcgtgcac caccggcttc ggtgatcccc agatgcagcg
    12001 gctgatcgat ctgttttgcc agcaaacgat aagactcaac agcgaggaag acgtcagacg
    12061 ctttcacgct gactttgaac tgatcgaagt tcaggcgatc gagatgatca acatgacgca
    12121 tggcggattc cagcaacgcc tgcggcgtcg gttcgccata cttttcttgc agatcttttt
    12181 ccagcgatcc ggcgttaacg ccaatacgga tcggaatgtt tttatcgcgc gcacagtcaa
    12241 ccaccatgcg aatacgctct tcattaccga tattgccagg gttaatacgc agacaatcga
    12301 cgccgtattc cgctactttc agcgcaatgc gatagtcgaa gtggatgtca gccaccagcg
    12361 gcacgttaac ccgctgtttg atgagtttga acgcttctgc cgcgtccatc gtcggtacgg
    12421 agacacggac gatatcagcg ccaacgcgtt ccagcgcctt gatttgattg accgttgctt
    12481 cgacatctgt cgtacgcgta ttggtcatgg actgtacggc gatgggagca ccatcgccaa
    12541 tcggcacatt cccaacgtaa atacgtgttg attttctacg ttgaattgga gcctggttat
    12601 gcatgaaaaa tctcccgcgt tacccgtctg ttactgcgcc ggtgattgtt cggcattgag
    12661 ggtcagacgc gcaacctggt tagttctgat aaaacgactc agatcgacag gtttcccttg
    12721 atactggatc tgtactgcgg ctggcgcacc aattttcagt ttatacggtg cctggccggt
    12781 taaattcaaa ttaccgtctt tacgctgcat cccgctaaac aattttttac cggtagcatc
    12841 agtgacctcc agccagcaat cggcagtaaa gttcatcacc agcgcattcg gatcagccgc
    12901 cggcgtggtc acgccagcct gatcggttgg caacggcgca gcaccatctg gcgttgttgc
    12961 tgccgttggt accggggtcg cggcggtatc aacatttgcc tgcgaaggcg aaacaaccgc
    13021 attctgttgc ggatcaacag ctggtgctgg cgcagttacg gcaggtgttt gcgtgttggt
    13081 tgcggtagta tccacagacg ctggcggcgt ggacgttgta gccgggtctg tagttgtcga
    13141 cgtatttaac gggacactct gcccctgctc gctattactg ctcagttctg ccgaagattg
    13201 atcggccata gtggtgatct cttcctgctg agctttgtgg tcttgccacc accaggcacc
    13261 gctcaggccg ataaccacaa acaacaccag ccaggtgaag gtcatcagcc agccatcacg
    13321 ttttttgcgg cgtttaccga gggaaaaact ctgcattggc gcaacttttg cagcccgtag
    13381 tggagcctgc ttttccagcc ctggcagcag ttcttcttct ggaatatgta ccagacgcgc
    13441 ataagagcgg atatatccgc gcaggaatgt tgaagcaaga tcggcgggtg ccttatcttc
    13501 ttcaatgtcg cgtaccgtgg aaaccttcag gcaaagtcgc tcggcaacgg cctgctgact
    13561 aagtcctagt tgttcgcgag cattacgcag gcgagcgccg gtagtaagtg cttcattttg
    13621 gtcgtgcgtg gcttcagtat tcattcgctg caggtacgtt taaatgagaa ttgagatgcc
    13681 ggtgaaccat catgctcacc cacaccgcga aacatccgtt aagttaaccg ttatcataca
    13741 gtataagacc gtctgtgtgc tcgtgacaaa gcccacattc actaaggcta acttactgtt
    13801 gcatcgttac atactgcctt aaagtcagca aaaacgcacc gttaatattg accagacaat
    13861 tgcaacttaa tacttcattc ataaaacatt acgccacggt acataaagta accgtggcgt
    13921 aatggctatc agaccgcttt aatgtcgatg gcttcaccct gcatccgttt acgcagggta
    13981 cgtttcgtac ggtcgataac atcgcccgcc aactgaccac aggcagcatc aatatcatca
    14041 ccacgagttt tacgcacaat agtggtgaaa ccgtagctca tcagcacttt tgagaaacgg
    14101 tcgatacggc tgttcgagct gcgtccatac ggcgcacccg ggaacgggtt ccacgggatc
    14161 aggttgatct tacacggcgt atctttcagc agttccgcca gttggtgcgc gtgttcagtg
    14221 ccgtcgttaa cgtggtcaag catcacgtat tcaatagtga ctcggccctg attggcgttg
    14281 gatttctcca gataacggcg caccgcagca aggaacgttt cgatattgta ctttttgttg
    14341 atcggcacaa tttcgtcacg aatttcgtcg ttcggcgcgt gcagggaaat tgccagtgca
    14401 acgtcgatca tatcgcccag tttatccagc gccggaacta caccggaagt agaaagcgtg
    14461 acgcgacgtt tagacaggcc aaaaccgaaa tcatcaagca tgatttccat cgccggaacg
    14521 acgttgttca ggttgagcag cggctcgccc atgcccatca tcactacgtt agtgattggc
    14581 cgctgaccgg tgacttttgc tgcgccgacg attttcgccg cccgccacac ctggccgata
    14641 atttccgaca cccgcaggtt gcggttaaag ccctgctggg cggtggaaca gaatttacac
    14701 tccagcgcac accccacctg cgaagagacg cagagcgtgg cacggtcgtc ttccgggata
    14761 tacaccgttt cgacgcgctg atcgccaacg gcgatcgccc atttaatggt gccgtcggat
    14821 gaacgctgtt cttcaaccac ttccggtgcg cggatctccg ccacctcttt cagtttgccg
    14881 cgcaacactt tgttgatgtc ggtcatctca tcaaagttgt cgcagcaata gtgatacatc
    14941 cacttcatca cctgatcggc gcggaagggt ttttcaccta aatctttaaa aaactcccgc
    15001 atctgctgac ggttgagatc cagcaggttg atttttccat ctttcgtggt gacgttttca
    15061 ggtgtgacta attgttcaga catatgctat tccggcctcg ttattacacg ttatggcccc
    15121 tggagggttg aaaaaagaaa cgccccggtg agcggctgct cgtccggggg cgctgcattg
    15181 tacaaattct ggcgcacgga tgccacgttt gcacgcggca tttacgaaat tattaacggg
    15241 tgcgcgggca cacttcgcct tcgccgaaga aataagcgat ttcgcgagcg gcagattcga
    15301 cggaatcaga accgtgggta ccgttttcgg tcaggctgtc agcgtaatca gcgcgcagag
    15361 taccggccag tgcgtttgcc ggattggtcg cgcccagcag atcgcggtga cgctgaacgg
    15421 cgttttcacc ttccagcacg gaaaccacga tcgggccaga ggtcatgaat tcaaccagac
    15481 catcaaagaa cggttttcca tcgtgttcag cataaaagcc acgagcctgt tcaacggtca
    15541 ggtgcagcat tttggtgccg acaattttga accctgcagc ttcaaagcgc gcaaagatat
    15601 taccaatgac gttttttgct accgcgttcg gtttgatgat ggaaaaagta cgttcaatag
    15661 ccatttttac ctctgtaaat tgttctgttg ttgtctgtac cagcgtacga gatggcgcgg
    15721 attataatga gcaacagggc cgttgactat tgatgaaggt aacatttttt taaaataaaa
    15781 tgagttttag caacaatcag ggacaaaaca gactattgca tgacaaattt cactgtcgcg
    15841 atttgcccca cgtcatccat caccagcaat tgataatcac ctttatccgt caaatgcagg
    15901 gtcacgttgc gcccgcgttc agttaacggt tcgccattca gaaaccacca gcgttcacct
    15961 gcccctccac ttgattgcaa cggcaaagtc gcttccgctg cgcccggtaa acgtttaata
    16021 atcgcgccat cgcgaacgcc tgtcagctgc agagggagct gggcatcgtg accgtatggc
    16081 gggcaaattg tcgaggccgg tggtaagcgc acagcgcgac gttctgatgc gggcagccag
    16141 ggttccagcg gtagcggcca gacatttacc atttcttgct tcgcctgcgg gcagtcagcg
    16201 gcgacgcgct gccctttgtc atccagccag atggggaagc gaatgccatt aatcccttcc
    16261 tgctctgaca gtaacagcgt tggcggctgg ctgccatcca gcagccaggt tgccaggcgg
    16321 cggcgacagt taccgtcacc ttccggcaaa gactgtccgc ccggccagca gataacgcca
    16381 cgagtgactg agttcgggcg cgggtcttcc ggcagattcg cactgcgcga cagtaagata
    16441 ttattgacct gattcaacaa tggtacggca ctggcaaagc caaactgacc aacaacgggc
    16501 gtgccgtcag gtctgccagt ccagatccca atgacatagc gagcgttaac cccaatcgcc
    16561 caggcgtcac gatagccata gctggtgccc gttttccatg ccagtggggc gacgcgcggc
    16621 aaggcactat ccggcaaggg ttgcgcttca tcagccataa tccggcgaat gatccacgcc
    16681 gcccccgacg acattaaagg ccgttcaagc agcggatcgt caggctgtaa gcgcaatttg
    16741 cctgccttgc cgtggcgagc aaacgcggta tacgctgccg ccatatcttc cagttttgca
    16801 ccagcgccgc cgagaatgag tgaaagattc ggcgcagcgc cgttgggcaa atataacggc
    16861 aatccaacat tgcgtaactt tgccgcaaac cgtttcggtc cataggcttc cagcacctgc
    16921 acagcaggta agttcagcga gcgcaccagc gcctcgctca tgctgatcgg gccatgaaaa
    16981 ccgctatcaa agttacctgg tcgataatca ccggtgcgcc gggggacgtc ttgcagcagt
    17041 gatgccgggt ggatcaagcc ttcatccagc gccagaccat aaacaaacgg tttgagcacc
    17101 gatcctggcg atcggatcgc attgaccata tcaacatgac caaagcgtga atcatcgttg
    17161 agatcaaccg atcccaccca gccgcgaaca cgcatatcgg tatgatcaac cacgatcatc
    17221 gccagtgagc tgcgcggtgg caaccgccct ttccagtttt gcgccagttc ttccagacgt
    17281 cgttgaagac cggcatccag cgtagtaacg attttgtcgc ttttgctttt accgagcatc
    17341 atgcgcgaaa acagcggtgc cagttgcggc atttgtcggg gtgccagcca gatgggttct
    17401 tcccttgact cttttacctg ctcacgggac cacacacctt gcgcagccat ccgttcgagc
    17461 actttattac gcgcggcttc ggcacgctcc ggccaacgat ccgggcgaag acggctgggc
    17521 gcttgcggca aaaccgccag cattgccgcc tcggaatagc ttaaattcgc aggcgacttt
    17581 ccgagataag cccaacttgc cgcaccgatc ccctgcaacg taccgccaaa cggagcgcgg
    17641 ttaagataca aggtcagaat ttcacgctta gacagatgcc attccagttg caacgcgcgc
    17701 cagagctggc gaattttgcc gccaaatgtt ttggggtgag gatcaagcag acgagcaacc
    17761 tgcatagtga gcgtgctgcc accggaaata acccgtcccg aagtgagatc ttgccatgct
    17821 gcgcgcgcca ccgagaatgg attcaccccc ggatgcttcc agaaccagcg atcttcataa
    17881 ttgatcagcg cttcaaggta acgtggagaa acatcttcga ttgttaccgg ataacgccag
    17941 atgccgtcag catcggcgaa gcgccagagc ggcgtaccat cctgcgccac gaccactcgt
    18001 gcgggattga cttcatgcag cggtagaggc cagagtttat ctgctcccca cgctgcaaga
    18061 aaaaggagaa atggcgcagc tgccagcgtt atccagcagc cgcgtttggt taacaagcga
    18121 ggcatttacg gtcggacaat cagcagatct tcagccgcgc cggtcgcccg ccattgggga
    18181 acatacattg attccaccat cggttgcggt acctgatacg ttcccggcgt caccgcccgc
    18241 gccagataca ccagcgttac cggttggtat tcatcaacgg caaccgccgc cacaaagcga
    18301 tcgtcacgga actcaatgtg cttaatactc gcctgctgca tctggttcag taagttctgc
    18361 acttcgccac cactttgctc caggctggcg ctaccgttcg ccagattctg gttttccagt
    18421 tccagaccca caggcagcag atccacgact aacgcatccg gcacgctgtt actggctttt
    18481 acctgcaacc acaccagcac cagatcgccg ctacgtaacg agtccagcga tttgctctta
    18541 ccatcagtac caagaatatg acgctcgatt tgcagcacat tgttcgccgg taaaggtgcg
    18601 gattgcggat aaccgctggc atccatacgc aaccataacg gctgatcgcc actgttgctc
    18661 acctgcaagg tgacaagttg atcgctattc agattgctgt tttgcgcttt ctcgcctgtc
    18721 agcggctcag ctgagaaaga ggtttgcgcc tgccatttac ccggtaaatc ctgaatcgta
    18781 cgggcagcca ggaacaacgc gttactttcc tgcgtcgata gccagcgttc accaaacgcc
    18841 tgctgcgaaa gtgtgttcag caaagtgtac tgctcatcgg gtagcagttt attttcttcc
    18901 agcaaggaga gcattaacgc gttgtcgcgc agtggactac cgtaatcacc cagccatatc
    18961 cgctcatcac tatttctcgg cgttttcagc gccagcgcaa tcgcttcttc accacgcgtc
    19021 gcatcaccca tggttttcag cgcaatgcca agttgcagca gcggtaaacc agaagcggca
    19081 tctgcacgat gctcccagat ttcacgcagc gcacccagtg gtgctttttg ctgacgggcc
    19141 agcaccagcg cagcgtaaga ctgaacggcg aatttactgg ctttgagatt atccgcgtac
    19201 gggatcgaca tcatgcccgg atcttgtaaa tagcgcagca gacgctcatt accccggtta
    19261 atggcgtctg tcggcacgct gtaaccctgt tcgcctgcgc ggaccaggaa atccatcacg
    19321 taagccgtca gccagtactc ttcgtcaccg tttttatccc acagcgcaaa gccgccgtta
    19381 tcacgttgca tttgcagcaa acgggaaatg ccgatatcga ccgatgcacg gcgtttctca
    19441 tcactgtcgc ctttgatgcc caacgcctgc agttgggctg cgttggtata aagtgacggg
    19501 aacaggccgc tggcggtttg ctcaagacag ccgtacggat acgcttttaa ctctttgata
    19561 taacgtgcaa tgttcagcgg tggtttgccg ctcaacaaca attgcccttc cagcgtaaca
    19621 ggcgagaagt tttgcaatcc atccgccgga atcgcccatg tctcaccagg ctgtaacgcc
    19681 gtaccgtaat taaccgtttg tgccgggaac gccggacgga cgccgatttt ccactgctta
    19741 tgctgatcgg caacggtttc acccggtaac gctaacccgc taatggtggc ctgaatttct
    19801 ccatcgccat aacccggcaa tgctcgcacc gggataaaca gcgtagtacg cacacctggc
    19861 gctaattcaa cggctgcggg tgaatcgctg accagttcaa gcaaaccact ggcggtcagg
    19921 gcaacgttca gtttttgcgg tttatcggta agattagtga tatccagcgt cagacgcgag
    19981 gtatcgccac tcgccataaa gcgcggcatg ttcagttcag caatcaccgg tgcggcaact
    20041 atcactttgc tttcgttgct accgaaatca tctgccgtcc aggcttgcgc catgacgcgc
    20101 aattcaccgt taaaatcgcc aatcggcagt gtaaccgagc cttcgccctg ttcgttgagc
    20161 gttaccgaca gcgcctgctg cgcgacaata ttgacgtgat tgaccggcgg tttaccacca
    20221 cgtttcagct catcaccatc gccaccgaaa cgcagagctg ccagacgccc ctgaccttca
    20281 ataacctgac cgtaaatatc gtaaatgtct gcgccatagc gtttctgacc aaagaacgcc
    20341 tgccacggat ctggcgtaac gtagtcagta atattcagca caccactatc aacggcagac
    20401 accagcacat tcacctgttt aggcttctcg ccatttttag tgctggcttt aattttcacg
    20461 gttaatggtt gattgggacg catttttgct ggtgtttcca gcgccagatc gaggcgacgg
    20521 ttttcatcac caagcggcag atgcaacaca ccaaccgcgc gttttggcgt cgcggagcga
    20581 gatttatcgc caggacgcac caccagcgta ctcaaataaa gatcatgacg attccaggtt
    20641 ttatcgaccg gaatcgtcag atccagccct tgagccggaa catcaatctc ttgccaccac
    20701 agcggccctt cactggactc gaccatcgca taacctttac ccgccgttgg cgcggcaata
    20761 tgcaacttaa tggtatcgcc ggggcgataa ctggctttat ctagtttcag cgtgacacgg
    20821 tcgggtcgca ctgcgccgct cccgtcgctg ttgtcctgcc agctatagcc agcccaaaaa
    20881 cgcacgctac tgaccgcttc attcggcgct ttgacttcca gacgataagc gccccactct
    20941 accgggaaac tgaccttacc ggtttcatcc gcctgaagat ccagagtttg ttcattttcg
    21001 atcagatctt tttgatcaaa ctgtgactgc cagccttcat cttctgacca gttccagtag
    21061 taatcgcggc gttcgcgaat caggcgcacc tgcaagcccg acacggcttt tttcacgcct
    21121 tgcgcatcgc tataaacgat gtcaaaagcg gcgttactgc cttcatcaac aatgggctgt
    21181 ttcaccgtgc tgtcagtgcg ataatcgtaa accgatttca aggcgaactg cggacggagc
    21241 cccggcaatg catcggcagg ccagatagcc tgctcagcgc ggcgcgtcac cgggcgaccg
    21301 cccgattcca gcaaactgcc ctggaaaata acctgcaatg gggaatgcgt ttccttccac
    21361 tggctttctg tagaaacttc gccgcgccct ttatcatcca gcgtcaactg aacttcatcc
    21421 agcgtgcggg aaaggttttc ggcagctata tcgccgaatt caaaaccagg taaggctgac
    21481 acagcctcac gcagtgggcg caggaaaagt tgcccttgca aagtattacc attagcaggc
    21541 gcaccgtaca ggtagtatcc caccacggag aatttcactt catcattcgg cgtaagcggg
    21601 gttttctcac cggtcagatt cagcgccata cgctctggca taaaatcttc gacgtggaaa
    21661 tcccacatcc ggtactggtt atcgcccgtg ttggcgcgaa tatgccacat accggttgcc
    21721 gcattgctat cgagtggcca ggtaaagtgg tagaggccat tctccggctg actaacgacg
    21781 ttcctgagta cctgcccgtc aggtttaatc acgtctaact tgatgggttg atcgggcaat
    21841 gctttaccgt ctgcatcgcg cagtaaacca ttgaggatta ccgtttcacc tgggcgataa
    21901 agatcgcgtg ggccaaacat gaaaaactgt ttgctatagc ctggcgcgcc agcaatgtta
    21961 aattctgcta agtccagcgc cggaagtttt aaatcgagta gcgttgtctg accgtcttta
    22021 cgtgccagca gtaatgccgc atttttatca ttttccagct gcacatgccc ctgagcgtca
    22081 ctggttgcct gagtcagagt ctgtcctttc tcatttaata aagagacttc aattccttgc
    22141 tgggccgcgc cgttttccag actttgggta aagatatcca gacgattgtg ataacggtga
    22201 gctgaaacgc cgatatcact taacgtaaac agcgtcgcgg gattactgta atcgtaacgt
    22261 ccagcctgat tcatcacagc cagatacacg cccgcctgct gaagcggttt gatatcgccc
    22321 agcggcagca ataatttttc acgggtgtta cgcgcaggat tgagatcaaa ccgtccggtg
    22381 tagaccagat ccgccatctg cagcagtttg tctgactgcc agttcgccag cgaattgcgg
    22441 tattcccatt ggctaatgaa tgctggcaga gattctggct taacgcggaa gaagttaaca
    22501 tcgacattat taacgttgag cgccattacc ggcagccctt caacgacttt gccaggcagc
    22561 agcgaaccac ggctggcaaa accgacgctg ggttggatgt cgcgggtagt tatagttttt
    22621 tcgtaatctt tactgaaggt tgcgttgttg agtgctttga cctccttgcc aatagtaacg
    22681 atcaaatcac gtttcggttc gaggtggcgt aaacgtagct ctttaagatt atctgacagc
    22741 tcccaggcac catccacttt gccgcttttt ttatcgacga catgaataac gcgtgagaaa
    22801 tcctgatccg ggtcgagagg gatggagaac gtcagcacca gcgtggctgc accatcaagt
    22861 tggacttcag agagatccag caatgtcagc accttaccgg cactctgttg ggccagtttt
    22921 tgtctctccg gcacggagag ttttgctgaa ctcgcgtttt ctgaagaggc cgctttagta
    22981 acttcagaag gcgcatcttt tttcaccgct gttggcgcgt tatcgttgtt gtcgcaccct
    23041 gccagcgcca gcattagcat gcaggcggct acgcgtaact ttttcatttt tcatccctgc
    23101 cacaatggcc cgttagcaac gtcgaataat tattatgcgt gagaatttac gttatgtaat
    23161 tcagtatcac gcccatacaa tccgaatttt agtgagaatt gtggctcaga cagccaaatc
    23221 agcctctttc gcttgtcgct tttcactaaa catccgacaa tttagcctaa ccccggcaaa
    23281 aatggagatg cctatgtcca cgacatggtt tgtaggagcc gactggctcg ccgaacatat
    23341 tgatgacccg gaaattcaga ttatcgatgc ccgcatggcg tcgcctggac aggaagatcg
    23401 taacgttgct caggagtatc tgaatggaca tattcccggc gcagtgtttt ttgatatcga
    23461 agcgctttct gatcacactt ccccgcttcc gcacatgctg ccgcgcccgg aaacgttcgc
    23521 cgtggcgatg cgtgaattag gcgttaacca ggataagcac ctgattgtct atgacgaagg
    23581 taatcttttc tcagccccac gagcatggtg gatgctgcgc acctttggtg tagagaaagt
    23641 gtcgattctg gggggtggac ttgcaggctg gcagcgcgat gatctgctgt tagaagaagg
    23701 tgcagtagag ctgccggaag gagagtttaa cgccgcgttt aatcctgaag ccgtggtgaa
    23761 agtaaccgat gtattattgg caagccatga aaatacggcg caaattattg atgcccgccc
    23821 ggctgcacgt tttaacgcag aagttgatga acctcgccca ggtttacgtc gcggacatat
    23881 tcccggagca ctgaatgttc cgtggacgga actggtgcgc gaaggcgaac taaaaacgac
    23941 cgatgaactg gatgcgatat tttttggtcg cggcgtcagc tacgacaaac caattatcgt
    24001 cagctgcggc tctggtgtaa cggcagccgt ggttttgtta gcactcgcga cgctggatgt
    24061 gccaaacgtg aaactgtacg acggcgcatg gagtgaatgg ggcgcgcggg cagatttacc
    24121 ggttgagcca gtgaaataag tattttacag gcaataaaaa accgccgaat ttggcggttt
    24181 tttattgctg gtctggttcg cggcctttcc agcaggttgt attaccgtag taatgcaagc
    24241 gcgtctcagc ggagacaata ctcgccagta actctctttt tgtcaagcaa aagagagtta
    24301 ttattgttct gttagtgtat tatccactgc ggccctttcc gccgtctcgc aagcgggcgc
    24361 tggcttaagg aaaggatgtt ccgcgaccgt tagagcaagc gcgtctcagc gcttgctatc
    24421 gcggcaatat cgccagtggt gctgtcgtga tgcggtcttc gcatggaccg cacaatgaag
    24481 atacggtgct tctgtatcgt acttattgtt tctggtgcgc tgttttccca agtaaataat
    24541 aaccggagtc tctccggcga caatttactg gtggttaaca accttcagag cagcaagtaa
    24601 gcccgaatgc cgccctttag ggcggcatat tttagattat ccggttttgt ttaaagtcac
    24661 gcaaaaaacc accccagcga cgttcataga atggcacaat atgttcggta ataaagtggc
    24721 taattccttt ttcccctttt ttcacctgac aaatatcgat tggttcatcg ccaggtaatg
    24781 tatcggtcgc tacacttccc gtcgcctgaa taatttcttc gatatcacca tcggcttcaa
    24841 tgccaataag taaattaggc tgtgcctctt cgttctcttt aattgaacaa ataaaagcac
    24901 gcttcaccgg cttaatggtt ttaaataagg tggtgagtga atcaatcatt tgtgctggcg
    24961 gctctgcgac ttccgataat atcagcgatt caccgccttc caggatttcc tggctgctca
    25021 gcggatttcc ctcttcacca atcaacaaac tgatttcacg cggcataaat tctttaccgg
    25081 tcggcagttt ggcattaagg aagagcgttt cgccaagggt catctcaaac aatgtgcgaa
    25141 cgggcattac gacaaatgcc tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg
    25201 tgaaaaaagg aatgacgctg gtgccgtctt ctttttccca gtgctgtaaa tcaagcgcac
    25261 tatcttcaac cacagcctcg ccctgcgccg ccgtaccagg cacccagacg gtggattcca
    25321 gtagagtacg gaaaaaggcc gggcggtgcg ccggttcggt tgctgctttt tccagcaggt
    25381 cttcaagttc gttttttgtt tcggacataa gaaccacaat tcattcaacg ttcggggcgc
    25441 aaatgcctga tgcgctacgc ttatcaggcc tacaagggat tcgcaatttg ttgaatttgc
    25501 agaatttgta ggccggataa ggcgtttacg ccgcatccgg catattagtt tacgccgtta
    25561 acagattagc tatcgtgcgc acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg
    25621 gcgctttacg gtaagtcgcc gagcagtcga tatgcagcca gccttgctga tagttctcaa
    25681 caaagtgcga caggaagccc gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg
    25741 tattgttcag ttcggcaaag ttagacggca gctggctgcg gtggaattcc gccagcggca
    25801 gacgccagaa tggttcgttc tcttgtgagg cactcgccag caaacgaccg gcaagcgcat
    25861 cgtcaaaact gaacagcgcg tgataatcat tacccagcgc agttttcgcc gccccggtga
    25921 gggtcgccgc atcaatgatc atttccggtt tctgcgcact ggcatcaatc agaccatcgg
    25981 caagcaccag acgcccttcg gcatcagtgt tcatcacttc aactttttta ccgttgcgat
    26041 aggtgatgat atcgcccagc ttgaacgcat tgccgctaat caggttatcc gcacagcaga
    26101 ggaacagctt cacgcgcttg ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa
    26161 ccgttgccgc gccgcccatg tccgacttca tcgagtccat aaacgcagtc tgtttgatgc
    26221 tgtagccgcc ggagtcaaaa gtgatacctt tacctaccag gcacgcgtac actggcgctt
    26281 ctttatcgcc agttgggttg taatccagcg ccagcaatac cggagaacgt tctgaaccgc
    26341 gtccgacggt gtgcagcccc atataacctt gctcacgcag atcttcgcct ttggtgatcc
    26401 gataagtcac acgatcgccc gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt
    26461 gcgatggtcc caattcttct gccggtgcgt tgatggtgtc acgcacccag tcgatgatca
    26521 tcaggcggtt atccagttcc tggcgctggg catcgtccag atccggccac tccactttac
    26581 gcgtgccttt cggggctttg taaccttgcc agaatgccca gcagcgatcc gcatcccagc
    26641 cttcaccgct taactgaaca tgcttgatgc ccagaccgtc aatcttgcgc gccgcacgct
    26701 ggatcagccc cagatcgtct gccccgttca aatgcagggt aatgccgtca ttattaatgc
    26761 tgtaagttgc tttttctccc cagcgcgcgt cggcaggttg ggtagagagg gtaatcttca
    26821 tcgcttctgt cattttagtt atccttcttt gtaaaagggc cgccagttgg cagccccagg
    26881 atttttccgc taaatgattc gcgttgcaga aaggcggcaa gtgagtgaag ccccaggagc
    26941 atagataact atgtgactgg ggtgaacgag cgcagccaac gcatctgcgg cgtgaagcat
    27001 gacgcggaaa ttattcggcc tcgtccagcc agactaacaa aatcgcttcg aggattttct
    27061 cgttggatgc ctgcgggtcg tcgtcgaaat cttccagatc gcaaatccac tgatgcatat
    27121 cggtgaatcg aaccgttttc ggatcaagat caggatacgc atcgtacagt gcttcgccaa
    27181 tttcgcggct atcggtccac ttaagtccca tactaacctc tgttaatgct cacgcgcatg
    27241 gttgatagtg taacgcggga tttcgactac taaatcttcg tcggtaacgc gtgcctggca
    27301 gcttaaacgg ctttcgggct ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc
    27361 tgagctttct ggcagcgagt caaaaccttc acgaacgatg cagtggcagg tggtgcaagc
    27421 acaggatttt tcacaggcgt gttcaatctc gataccgtta cgcagagctg cgtcgagaat
    27481 ggtttcaccg ctattagctt ccagaacagc gccatcagga cagagatcct gatgaggcaa
    27541 aataacaatc tttggcatat taaacctcgt ccacggaatg gcctttcagc gcacgacgaa
    27601 ccgactggtc catgcggcga gcggcgaaat cctgggtttg tttgtctacg tttttaatcg
    27661 cttgttcgat ggcgtcaacg tcatcgccct gcgccacttc actcaggtga gcggcagcat
    27721 cgtcaatgac ctgacgttct gcggcgctta acagcgcggc atcagcagcc agcgcgccgt
    27781 gcagactttc cagcacacgc gccgcttcta ctttttgttc tgccagcatt cgggctttta
    27841 cgtcctgctc ggcatagctc attgagtctt tgatcatcga agcgatttcg ctatcggtca
    27901 gaccgtaaga cggtttgacc tgaatagacg cctcaacgcc ggtggatttc tccatcgccg
    27961 tcacgctcaa aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc gcaccaccag
    28021 ccggtagcgc cggaatacca cgcagcgcaa aacgcgccag tgagcggcag tcctgcacca
    28081 gttcgcgctc accctgcatt acatggatag acatcgccgt ctgaccatct ttaaaggtgg
    28141 tgaaatcctg agcgcgggcc accggaatag tggtattacg cggaatcact ttctccacca
    28201 ggccgcccat cgtttcgagg cccagcgaca gtgggatcac atcaagcagc agcatttcgc
    28261 tgtctggctt gttacccacc agaatatccg cctgaatcgc cgcgccaata gcgacgactt
    28321 tatccgggtc gatggaagtc agcggtggac gaccgaaaaa ttcgcctacc cgttcacgca
    28381 ccagcggcac gcgagtagaa ccgcccacca tcaccacttc cagcacttcg tcagcttcta
    28441 cacccgcgtc tttaagcgcg cgacgacaag ccagtaaggt tcgttttacc agtggcgcga
    28501 tcagttcatt gaattgttca cggctgattt cgccctgcca gcccgcaacg ttaacggtca
    28561 cggagtccgc atcgctcagc gcgattttgg ctgcaatggc ggcatccagc agttcacgct
    28621 gaacgcggtt atcgctacga tcaggaatgc ccgcctgctc gcgaatgtaa tccgccagca
    28681 gatggtcgaa atcatcgccg ccgagcgcgg aatcaccgcc ggttgccagc acttcaaaca
    28741 cgccgcgact taagcgcaga atggaaatat caaacgtccc gccaccgagg tcataaacag
    28801 cgatcacgcc ttcctgaccg gaatccagcc cgtaggcgat agccgcagcg gtcggttcgt
    28861 taagtaagcg cagaacatgc aaccccgcca gacgcgccgc gtctttggtg ccctgacgct
    28921 gggcatcgtc aaagtacgcc ggaacggtga taactacacc atccagctcg cctgccaggg
    28981 cttcagttgc ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc accgggttca
    29041 gcagccccgc cgccgtttca atcatcggca ggccgttttc gctggcctgg aattgataag
    29101 gcagatgcgg atagcgttgc tggatatcag ccagcgagcg ccccatcagg cgtttaacag
    29161 aactaatggt gttggcggtg tcaagcgctg cgttagtacg cgcgtcataa cccaccgaat
    29221 gcccttgctg ttgatagtga acaacagatg gcagcaggtg acggccttca tggtcggcta
    29281 acgtttcggc ctgaccgctg cgcactgtcg ccaccagcga gttggttgtg cccaggtcaa
    29341 taccagccgc cagacgacgc tgatgcggcg cagcactcaa accaggttca ctaatttgta
    29401 ataaggccat gtttagcttc cagaaattaa aaatcgagca gtttttcttc gagttgttcg
    29461 gcactgctcc gcagtttatc gagaaaacgc agcttacgca cggtatccgc cgccgcgtcc
    29521 cacgcctcgt tgtctaactg ttcaaccatc aactgatggc gggtatcaaa catctttttc
    29581 acacgtttga taaagctttc cagccgcgct tcatctttcg cctgttcgat ctcgtccagc
    29641 tcttcgcgca gctccaactg ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg
    29701 ctggcgagat caaaaccgtg taaagaaagc aaatattccg cgcgcattaa cggatgacgc
    29761 agcgtttgcc aggcctggtt aatggttgca gattgctgta cggcggcgag ttgttccgcc
    29821 tggcttccgc tggcgaattt atcaggatga tactgacgtt gtagatcctg aaaacgcagg
    29881 ctcagcgcct gggtatcgag ttgataacgg gcaggcaagc caaagaaggt gaagtaatcc
    29941 ataacattct cagggctgcg atccgccgcg ataacggcga atcaggtaaa acaaacccca
    30001 cgcgcaggcg accacggtgg ggttatcggt atgcgcatca aacgtggaag ctttcgccgc
    30061 aaccacactc atctttgacg ttcgggttgg tgaatttaaa cccttcgttc aggccttctt
    30121 ttacgaagtc cagctgcgta ccgtccagaa attgcaggct tttgccatcg accacgactt
    30181 tcacgccttt gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta
    30241 cataagccat acctgaacat ccggaggttc tcacgcccag acgcaggcca aaccctttgc
    30301 cgcggttagc cagaaaggta tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca
    30361 taaccaaacc tcaactctta ttttgcttca cgtttgcttt tatagtccgc aatggcggct
    30421 ttgatcgcgt cttctgccag aatagaacag tgaattttca ccggcggcag ttcaagttct
    30481 tcagcaatat cggtattttt gatcgcctgc gcttcgtcga gagacttccc tttcacccat
    30541 tcggtgacca gggagctgga agcgatagca gaaccgcagc cgtaagtttt aaaacgcgcg
    30601 tcttcaatga taccttcatc gttgacttta atctgcaact tcatcacgtc gccacaggcc
    30661 ggtgccccca ccatgccgct gccgacgttc tcgtcgttgt tgtcaaagga acccacgtta
    30721 cgcggattct cgtaatggtc gataactttt tcgctgtaag ccattataaa ttctcctgat
    30781 tccgataccg attaatgatg agcccattcg atgctgttca gatccacgcc ctgcttgtac
    30841 atttcccaca gcggagaaag gtcacgcaga cgaccgatgg atttacgaac taactcgatg
    30901 gtgtagtcga tctcttcttc agtagtaaaa cgacctaaag agaaacggat agagctatgt
    30961 gccagctcgt cgttcagccc cagcgcgcgc agcacgtagg acggttcgag gcttgctgac
    31021 gtacaggcgg aacctgaaga aactgcgagg tctttcagcg ccataatcag cgactcacct
    31081 tcaacgtagt tgaagctgac gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc
    31141 aggtaaactt cttcgatatc tttgatgccg ttccacagac ggttacgcag gccgcgcaga
    31201 cgttccatct cggtcgccat ctcttctttt gcgatgcgat aggcctcgcc cattccgacg
    31261 atctggtgaa caggcagagt gccggaacgc ataccgcgct cgtgaccgcc gccgtgcatt
    31321 tgcgcttcga tgcgtacgcg cggtttacga cgtacataca gcgcaccgat acctttcggg
    31381 ccatagattt tgtgaccgga gaaagacatc aggtcaactt tcaactggct caggtcgata
    31441 ggcagtttgc ccacgctctg ggttgcatca acgtgataga taatgccacg agcacggcac
    31501 atttcgccga tagccgcgat atcctgcacc acgccgattt cgttatttac gtgcatgatg
    31561 gaaacaagga tggtgtcatc acgcatcgct gcttcaagtt ctttcagatc gataatgccg
    31621 ttacgctgcg gtgccaggta ggtgacttca aaaccttcgc gctccagctg acggcaggta
    31681 tccagtaccg ctttgtgttc ggttttgctg gtgatgatgt gcttgccttt tttctgataa
    31741 aagttggctg cacctttgat cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg
    31801 atttcacgcg gatcagcgcc gaccagatcg gcaatctgat tacgggcgat atctaccgct
    31861 tcttcagcct gccagccgaa acggtgagaa cgggaggccg ggttaccaaa ggttccgtcc
    31921 atcgtcataa actgcatcat tttctcggca acacgcgggt ccaccggcgt ggttgcggag
    31981 tagtcgagat aaatcggtaa tttcattgct ctataaactc cgtacatcac tcaatgcaag
    32041 gaaccaggct accggctgga tgtacgaccg tgtttacgga gcgcgatacg ccccggcctg
    32101 attctaatat cttgttatct ttaacctaac tgtttcgggt ttcagcaagt tgacaaactt
    32161 acgaatctcc gggagcttac ataagtaagt gaccggagtg agtatgtgca gtcaacgcag
    32221 atgcaacacg aaacaggacg gttaaattaa gcgcgtaact taacgtcgat cgcgtcttgt
    32281 atgcgggtgc gtggcgcgtc gtgagtatgc tgacgaccag acacatccag cacttcctgg
    32341 ttattaacca gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg gtcgctcaaa
    32401 tcacgccaca gcgcgtgggt caggcattta tcgccgccct ggcaaccgcc tttaccctga
    32461 caacgggtag catctacaga ttcgtcaacg gcgctaatca cttcgccaac ggcgatgctg
    32521 ctggcatctt tgcctaacag ataaccaccg cctggtccac gtacgctgga aaccagacca
    32581 tttttacgca gacgggaaaa cagttgttcc agataagaaa gggaaattcc ctgacgttcg
    32641 gaaatatcag ccaacggtac cgggcccgct tcagagttga gcgcaacgtc aagcattgcg
    32701 gtcacggcat agcgcccttt agatgtcagt ctcatgtctt acttcacctc aaactcgccc
    32761 ctgcccgggg ttttttattg taaaagtggg ggtattgcat agcagggtca agtctgacat
    32821 tcccgagtaa attggtcaac tatttacttg actgatttag tcgggtattt aaccttcagt
    32881 gccatttttt tatcgtggcg tttgtacctg ttgtccttcc agaccatggg cgggttgagg
    32941 gcaattattc gcattagcgc tggcctcttc ccctcaccct aaccctctcc ccgaaggggc
    33001 gaggggaccg tccgtgcatt tatctggctt agccctcagg ccttattacc tttattctgc
    33061 tgctctatag aagccagaat cccgcgcagg atattcaatt cctggctttc cgggcgcgca
    33121 cgggtaaaca gacggcgcaa tttattcatc acctgccccg gatggttttc acggataaaa
    33181 ccggttgcca gcagcgtttg ttccagatga ccataaaaac gctccagatc atcgaccagc
    33241 ggatacggcg tctcttcatg ctcgacctgc tcgccatttt cctgagttgc cagccaggcc
    33301 atacgcactt cataggcaat aacctgaacc gccattgcca agttcagcga actgtattcc
    33361 gggtttgccg caatcgcgac atgataatgg catttctgca actcttcatt ggtcaagccg
    33421 acgcgctcac gaccaaacac cagcgccacc ggggtatttg ccgcttcagc gacgcttttc
    33481 agaccgcatt cgcgcgggtc gagcatcggc cacggcagcg tgcgggagcg tgcgctggtg
    33541 cccaccacca ggctacaacc ggctaacgct tcgtcgagcg tatcgacgat gtgagcatta
    33601 ccaatcacat cgctggcccc tgctgccagg gcaatcgcct gggagtcggg tttcaccagt
    33661 ggattaacca gccacagatt ggttaatccc attgttttca tggcacgggc aacagaaccc
    33721 atattgccgg tgtgtgacgt ctccaccagc acaattcgaa tattttgcag cattgtcttt
    33781 cttcatctaa agattattca cgcatcttat cataaaacga agacagatgc cgatctcgct
    33841 gctatactct gcgccgtttt cccgttcttt aacatccagt gagagagacc gatgcatccg
    33901 atgctgaaca tcgccgtgcg cgcagcgcgc aaggcgggta atttaattgc caaaaactat
    33961 gaaaccccgg acgctgtaga agcgagccag aaaggcagta acgatttcgt gaccaacgta
    34021 gataaagctg ccgaagcggt gattatcgac acgattcgta aatcttactc acagcacacc
    34081 atcatcaccg aagaaagcgg tgaacttgaa ggtactgatc aggatgttca atgggttatc
    34141 gatccactgg atggcactac caactttatc aaacgtctgc cgcacttcgc ggtatctatc
    34201 gccgttcgta tcaaaggccg caccgaagtt gctgtggtat acgatcctat gcgtaacgaa
    34261 ctgttcaccg ccactcgcgg tcagggcgca cagctgaacg gctaccgtct gcgcggtagc
    34321 accgctcgcg atctcgacgg taccattctg gcgaccggct tcccgttcaa agcaaaacag
    34381 tacgccacta cctacatcaa catcgtcggc aaactgttca acgaatgtgc agacttccgt
    34441 cgtaccggtt ctgcggcgct ggatctggct tacgtcgctg cgggtcgtgt tgacggtttc
    34501 tttgaaatcg gtctgcgtcc gtgggatttc gcggcaggcg agctgctggt tcgtgaagca
    34561 ggcggcatcg tcagcgactt caccggtggt cataactaca tgctgaccgg taacatcgtt
    34621 gctggtaacc cgcgcgttgt gaaagccatg ctggcgaaca tgcgtgacga gttaagcgac
    34681 gctctgaagc gttaatgact caggcgggtg atgtcactca cccgccctcg cctttcaggc
    34741 gctattctga aatacttcct caccgcttta ctttctttcg tcactctccc accatctttc
    34801 cccgattaat ggatgataaa gaagtcacaa ggaaaggtta tggcgctgcc agtgaacaaa
    34861 cgcgttctca aaattctgtt tattctcttt gttgttgcct tctgcgttta tttagtgccg
    34921 cgcgttgcca tcaacttctt ctattatcct gacgataaaa tttacggtcc cgacccctgg
    34981 tcggcggaat ccgtcgaatt tacggctaag gacggtactc gtctgcaagg ctggtttatt
    35041 ccttcttcga cgggccctgc tgacaatgcc atcgcaacca tcattcatgc tcacggcaat
    35101 gccggaaata tgtccgccca ctggccgctg gtcagttggt tacccgagcg taatttcaac
    35161 gtttttatgt ttgattatcg cgggtttggt aaatcaaaag gcacgccgtc ccaggccgga
    35221 ttgctggacg atacgcaaag tgccatcaat gtggtgcgcc atcgcagtga tgtaaaccca
    35281 caacggctgg tgctgttcgg gcagagtatt ggcggggcga atattcttgc tgttattggt
    35341 caggatgatc gtgaaggcat acgtgcggtg atcctcgact ccacatttgc ctcttatgca
    35401 accatcgcca atcagatgat ccccggcagt ggctacttac ttgatgagag ttacagcggc
    35461 gaaaattata tcgccagcgt cagcccgatc ccgcttttac tcattcacgg taaagctgat
    35521 cacgttatcc catggcagca cagcgaaaag ttgtatagcc tggcaaaaga gacaaaacgg
    35581 ttgatcctaa tcccggatgg cgaacacatt gatgcttttt ccgatcgtca cggcgatgtt
    35641 tatcgcgaac agatggtgga ctttatcctt agcgcgttga atccgcagaa ctaacccatg
    35701 atcgctagca cgataatcat tcacaaaacc accttaagac atgctaatcc actggtcaga
    35761 acagtttaag atgagaaaaa ttctgtgacg cttgccaaca tttctgatga ttagcattcc
    35821 cttcgccatc tccctgagca aactttagct attcttatca attatgctta tgggagatct
    35881 acagatgatg cctacgcttg ctccaccatc tgtcctttcg gctccccagc gccgctgcca
    35941 gatcttgctg acgctctttc agccggggtt gaccgccacc atggcaacct tcagcgagct
    36001 taatggtgtg gatgatgata ttgccagtct tgatatcagc gaaacaggac gggagatcct
    36061 gcgctatcat caactcacac tgacgactgg ttatgacggt agctaccggg ttgaaggtac
    36121 agtgcttaac caacgtttgt gtttatttca ctggctacga cgtggtttcc gtctgtgtcc
    36181 gtcatttatt accagccaat tcacccccgc cctgaagagt gaactgaagc ggcgcggaat
    36241 tgcgcgtaac ttttacgacg ataccaatct acaagcgtta gtgaatctct gctcccgacg
    36301 gctgcaaaaa cgctttgaaa cgcgcgatat tcatttcctg tgtctgtatc tgcaatattg
    36361 tttgctgcaa caccacgctg gaattacgcc tcagtttaat ccgctccaac gtcgctgggc
    36421 ggaatcctgc cttgaatttc aggtagcgca ggaaattgga cgccactggc agcgtcgggc
    36481 gctccagcct gtaccgcctg atgagccact gtttatggca ttactttttt ccatgttgcg
    36541 ggttcccgat ccattgcgtg atgcgcatca gcgagacaga caattgcgtc agtctatcaa
    36601 acgtctggta aaccattttc gtgagctggg aaatgttcgt ttttatgatg aacaggggtt
    36661 atgcgatcag ctttataccc acctcgccca ggcgttaaat cgcagtttgt ttgccatcgg
    36721 tattgataat accctgccgg aagagttcgc cagactgtac ccacgcctgg tgcgcaccac
    36781 ccgcgcggcg ctggccggat ttgaaagtga atacggcgtc catctttctg atgaggaaag
    36841 cggtctggtc gcggtgattt tcggtgcctg gctaatgcag gaaaacgatc tgcatgaaaa
    36901 acagattatt ctactgaccg ggaatgatag cgagcgagaa gcgcagattg agcagcagtt
    36961 acgcgaacta acgttactgc cgctcaacat taagcatatg tcggtaaagg catttttgca
    37021 gacaggcgct ccgcgtggcg cggcactgat tattgcgcct tataccatgc cattaccgct
    37081 cttttcacca ccgctgatct atacggacct gacgctgaca acacatcaac aggagcagat
    37141 ccgcaaaatg ctcgaatcag catgagggaa caactttcgg gcgcaaaaac attgccggaa
    37201 gcgccaccag cgccattacc cagaacacgc cgtggcccag atattgatac aggaaaccgg
    37261 cgaaaacggt catgatagcg atactgccgc ccatcgcgac ggcagagtaa accgcctgta
    37321 aacggatgac ttcgctaccc tggcgaacag caatatagcg catggcggcc aggtggcaga
    37381 ccgtgaaggt gccgcaatgc aaaatttgca ccactatcaa ccacggcaac gccgtagttg
    37441 ctcccataat gccccagcgc actacgccgc aaatcgccga gatcaacagc atatcgcgag
    37501 cactacaacg gcggaaaagt ttattactca gcgcaaagat aatgacttcc gccaccacgc
    37561 ccagcgacca caaatacccc accgccgagg ccgagtagcc agctgcctgc cagtaaatgg
    37621 cgctaaaacc gtaataggcc gaatgtgccc cctgcaataa acaaacgcag gccagaaagc
    37681 gccagttctg gcgaaccagc gccaaccacg cagaccaacc ggtgctctcc tgctggcgac
    37741 ttgccccttg tggctgaatc gtcggacgga tgagaaagcc gagcagcatg gatgccacgc
    37801 ccaacgtcaa cagcgcgagg atcacccgat aatcaaacat agtgaccagt ttgcccgtca
    37861 gcgccgagcc aatgacaaac gccaccgagc cccacagtcg cactttgccg taatcaagcg
    37921 ggaactgctt ttgccacgta ttcgccagtg catcggtcaa cggtaccagc ggtgagaaaa
    37981 agaggttaaa gccaatcatc accagcatca gccacgctac gtgcgccccc gcccagaagg
    38041 cgacagcaaa gagaagtgtc agcagtgcca gcacgcgcaa ggcggaaatc aggcgggaag
    38101 gatcgctgac gcggggcgcg atgagcaaac tcccgagaaa acgggcaacc agacctgccc
    38161 ccaataacag gccgatggtt tctggcgtta aaccaatccc tttaagccag acgctccaga
    38221 aaggtagaaa aatgccgtaa ctaaaaaagt atgtgaaata gccgagcgcc aaccagcgcg
    38281 tggattgcaa aaccatgagt ccctcccgtc aggaggcgtt agtctggggt taatcgaatg
    38341 ttggtgcaag ttgaaaaata agaatattcg agcctgcttc ccgatgtctg taacgccagt
    38401 tcacaagcac taaaaaactg cgatccgcga cgcatgttgc aaaaataatg cgtaattatg
    38461 ccgttacgct tgccaaacgt tcctgcacaa tggcgatgaa atcgcgtaat gccggcttca
    38521 tctccccttt cttccacgcc atcagtaaag caatggaagg cacatttccg gcaatgggac
    38581 gaaaaacaac ctgcccggta ttaaaattat tcatataacc gggtatcaaa gtgacgccca
    38641 gccccatgcc caccagattc atggtcacca gaatattcgt tgccacctgg acgatatttg
    38701 gctggctgtt ttcttgcgca aaccacgctt taacgatcgg cgcaagcgaa ccggaatacg
    38761 ccggatcggt actgacgaaa ttcacgccat ccagttgcgc ggcggtgatc tctttttcat
    38821 gtgctaaagg gtgatcaacc ggtaacacca ccactaatgg ttcgtcaaaa agctccagat
    38881 aatcaatctc cgggctataa acaggatggc gcattaagcc gacgtcgagt tcaccacggc
    38941 gaattttttc ctcctgttgc gtggtgatta aactcaccag ctcaatcaag gtgtctggct
    39001 gtctgagacg aaacattggt aatacttttg gcaataaatt cacttccgcc gatggcacaa
    39061 agccaatggt taattgtctg tcttcctgaa caattttccg cgcccgtaat ttggcatttt
    39121 ccgcttgttc gaggattgcc agcgcgtcct ggagaaaaca ttctcccgcc gccgtcaacg
    39181 cgactttgcg cttatccctc accagtaacg gaacaccgac acagttttca agatcgcgga
    39241 tctggctgct taacgaaggc tgtgaggtat gcagtttttc cgccgcacgg gtaaagttca
    39301 gtgcctgcgc cactgcgacg aaatagcgta aatgccgtag ttccatcacc ttccccttgt
    39361 tatcgaaaaa acgtctcagc tggtagaaat taactatttc acatattagc aaccaaccag
    39421 caacatcctt atgacacaaa aatagaaggt caatacatct tatctttcag gattaaaaaa
    39481 tatgaccaca ccctcagatt taaacattta ccaactgatt gatacccaaa atggtcgggt
    39541 cactccgcgt atttataccg acccagatat ttaccaactg gagcttgaac gtattttcgg
    39601 ccgttgctgg ttatttctcg cccacgaaag ccagatccca aaacctggtg atttctttaa
    39661 cacctacatg ggggaagatg cggttgtcgt agtgcgtcag aaagacggca gcattaaggc
    39721 gtttctcaac caatgccgcc accgggccat gcgtgtgagt tatgcagatt gcggcaacac
    39781 tcgcgccttt acctgtccgt atcacggctg gtcttatggc attaacggcg agttgatcga
    39841 tgtaccgctg gaacctcgcg cctacccaca agggttgtgt aaatcccact ggggactaaa
    39901 cgaagttcct tgtgtggaga gttataaagg gctgattttt ggcaactggg ataccagcgc
    39961 accgggcctg cgtgattacc tgggtgacat tgcctggtat ctggatggca tgctggatcg
    40021 tcgcgaaggc ggcaccgaaa ttgtcggcgg cgtacaaaag tgggtgatca actgtaactg
    40081 gaaattcccg gcagagcagt tcgccagtga ccagtatcat gctctgttca gccatgcttc
    40141 tgccgttcag gtattagggg cgaaagatga tggcagcgat aagcgcctcg gtgatggaca
    40201 aaccgcccgc ccggtgtggg aaaccgccaa agatgcgctg caatttggtc aggacggtca
    40261 cggtagcggt ttcttcttta ctgaaaaacc ggatgctaat gtctgggtcg atggcgcagt
    40321 ttcaagctat taccgcgaaa cctatgccga agcagaacaa cgtttaggtg aagttcgcgc
    40381 cctgcgcctg gcgggtcata acaatatttt ccccacgctt tcatggctca acggcactgc
    40441 cacgctccgc gtctggcatc cgcgcggccc tgatcaagtt gaagtgtggg cgttctgtat
    40501 tactgacaaa gccgcctccg atgaagttaa agccgctttt gaaaacagcg ccactcgtgc
    40561 ttttggtcct gctggttttc tcgagcagga tgactcggag aactggtgtg aaatccagaa
    40621 attgcttaaa ggccaccgcg cccgcaacag caaactgtgt ctagaaatgg ggcttggtca
    40681 ggaaaagcgc cgcgacgacg gcattcctgg cattactaac tatatctttt cagaaactgc
    40741 cgctcgtgga atgtaccaac gctgggccga tcttctgagt agcgaaagct ggcaggaagt
    40801 gctcgataaa accgccgctt accagcagga ggtgatgaaa tgagtgcgca agtttcacta
    40861 gagttacatc accgcattag ccagtttctc tttcacgaag ccagcttact ggacgactgg
    40921 aaatttcgtg actggctgga gcagctcgac aaagagattc gttacaccat gcgcaccaca
    40981 gttaacgcgc aaacacgcga ccgccgcaaa ggcgtccagc caccgacaac ctggattttt
    41041 aatgacacca aagaccagct ggagcggcga atcgcccgtc tggaaacggg catggcctgg
    41101 gcagaagagc cgccgtcacg cacccgtcac ttaatcagca actgccagat aagcgaaacc
    41161 gacatcccaa acgtatttgc tgtgcgggta aattatctgc tttatcgggc acaaaaagag
    41221 cgcgatgaaa cattctatgt tggaacgcgt ttcgacaaag ttcgccgtct ggaagatgac
    41281 aactggcgct tgctggaacg ggatatcgtc ctggatcaag cggtaatcac ttcccataac
    41341 ctgagtgtac tgttctgatg aatcgaattt atgcgtgtcc cgttgcggat gtgccggagg
    41401 gtgaggctct ccggatcgat acctcgcccg tcatcgccct gttcaacgtt ggcggcgagt
    41461 tttatgccat taacgatcgt tgcagccatg gtaatgcgtc gatgtcagaa gggtatctgg
    41521 aagatgacgc cacggtggag tgcccgctac acgccgccag tttttgcctg aaaacgggga
    41581 aagcgttatg cctgcccgcc accgatccgc tcaccactta tccagtacac gttgaaggtg
    41641 gtgacatttt catcgactta ccggaggcgc agccatgagc gatctgcata acgagtccat
    41701 ttttattacc ggcggcggat cgggattagg gctggcgctg gtcgagcgat ttatcgaaga
    41761 aggcgcgcaa gttgccacgc tggaactgtc ggcggcaaaa gtcgccagtc tgcgtcagcg
    41821 atttggcgaa catattctgg cggtggaagg taacgtgacc tgttatgccg attatcaacg
    41881 cgcggtcgat cagatcctga ctcgttccgg caagctggat tgttttatcg gcaatgcagg
    41941 catctgggat cacaatgcct cactggttaa tactcccgca gagacgctcg aaaccggctt
    42001 ccacgagctg tttaacgtca acgtactcgg ttacctgctg ggcgcaaaag cctgcgctcc
    42061 ggcgttaatc gccagtgaag gcagcatgat tttcacactg tcaaatgccg ccaggtatcc
    42121 tggcggcggt ggcccgctgt acaccgccag taaacatgcc gcaaccggac ttattcgcca
    42181 actggcttat gaactggcac cgaaagtgcg ggtgaatggc gtcggcccgt gtggtatggc
    42241 cagcgacctg cgcggcccac aggcgctcgg gcaaagtgaa acctcgataa tgcagtctct
    42301 gacgccggag aaaattgccg ccattttacc gctgcaattt ttcccgcaac cggcggattt
    42361 tacggggccg tatgtgatgt tggcatcgcg gcgcaataat cgcgcattaa gcggtgtgat
    42421 gatcaacgct gatgcgggtt tagcgattcg cggcattcgc cacgtagcgg ctgggctgga
    42481 tctttaagga agcacgatga aagaaaaaac gatcattatt gtcggtggcg ggcaagcggc
    42541 ggcaatggct gcggcctcgc tacgccagca agggttcacc ggtgagctgc atctgttttc
    42601 cgatgagcga catcttcctt atgaacgacc tccactctcg aaatccatgt tgctggaaga
    42661 ttccccgcag ttacagcagg tgttacccgc taactggtgg caggaaaaca atgttcatct
    42721 gcattccggt gtaaccatca aaacgctggg tcgcgacaca cgagagttag tgttaaccaa
    42781 cggcgaaagc tggcactggg atcagctttt tatagcaatc ggcgcggcag ctcgaccgct
    42841 gccgttgctt gatgcactgg gagaacgctg ctttacccta cgccatgccg gtgatgccgc
    42901 cagactgcga gaagttctgc agcccgaacg gtcagtcgtg attgtcggtg ccggaactat
    42961 tggtctggaa ctggctgcca gcgccacgca gcgcagatgt aaggtgacag tgattgaact
    43021 ggcggcaacc gtcatgggcc gtaatgcacc accgcccgtg caacgctatc ttttacagcg
    43081 ccaccagcag gctggtgtgc gcattctgct caataatgcc attgaacatg tggtcgatgg
    43141 tgaaaaagta gaactgacgc tgcaaagtgg ggaaacgctt caggctgatg tggtgattta
    43201 cggtattggt atcagcgcca acgagcaact ggctcgcgag gccaaccttg atactgccaa
    43261 tggcattgtc attgatgagg cttgccgcac ctgcgatccc gcgatctttg ccggtggcga
    43321 tgtggcaatc actcgtcttg ataatggtgc actacaccgc tgcgaaagct gggaaaacgc
    43381 caataaccag gcgcaaattg ccgctgccgc aatgttaggg ctaccgctac cgctactgcc
    43441 gccgccgtgg ttctggagcg atcagtacag tgataactta cagtttattg gcgatatgcg
    43501 tggcgatgac tggctttgtc gtggcaaccc ggaaactcag aaggcgattt ggtttaatct
    43561 gcaaaacggc gtgcttatcg gtgcggtcac gctgaatcag gggcgtgaga ttcgcccaat
    43621 tcgcaaatgg atccagagcg gcaaaacgtt tgatgcgaaa ctgctgatag atgagaacat
    43681 cgcgcttaaa tcactgtaac caggataatt agcgaatatc tcaacgcctg gggcgtggcg
    43741 aggtgcaaga gtgtgtatta cgtttaaatc acattatctt gcaaagggat tggttatgaa
    43801 cacactacgt
//
LOCUS       DAZOZY010000012        42133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZOZY010000012 DAZOZY010000000
VERSION     DAZOZY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.012"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="ACHURQ_000585"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="ACHURQ_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR0822451.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHURQ_000586"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHURQ_000586"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR0822452.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHURQ_000587"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHURQ_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR0822453.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="ACHURQ_000588"
     CDS             4216..4620
                     /locus_tag="ACHURQ_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR0822454.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="ACHURQ_000589"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="ACHURQ_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR0822455.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="ACHURQ_000590"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="ACHURQ_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR0822456.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHURQ_000591"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHURQ_000591"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR0822457.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHURQ_000592"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHURQ_000592"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR0822458.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9472
                     /locus_tag="ACHURQ_000593"
                     /pseudo
     CDS             8043..9472
                     /locus_tag="ACHURQ_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9542..10318
                     /gene="yaaA"
                     /locus_tag="ACHURQ_000594"
     CDS             9542..10318
                     /gene="yaaA"
                     /locus_tag="ACHURQ_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR0822459.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10471..10767)
                     /locus_tag="ACHURQ_000595"
     CDS             complement(10471..10767)
                     /locus_tag="ACHURQ_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR0822460.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="ACHURQ_000596"
     CDS             complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="ACHURQ_000596"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR0822461.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="ACHURQ_000597"
     CDS             complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="ACHURQ_000597"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR0822462.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="ACHURQ_000598"
     CDS             complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="ACHURQ_000598"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR0822463.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15691..15810)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="ACHURQ_000599"
     CDS             complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="ACHURQ_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR0822464.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16025..16711)
                     /locus_tag="ACHURQ_000600"
     CDS             complement(16025..16711)
                     /locus_tag="ACHURQ_000600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0822465.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17111..17251)
                     /gene="yjjY"
                     /locus_tag="ACHURQ_000601"
     CDS             complement(17111..17251)
                     /gene="yjjY"
                     /locus_tag="ACHURQ_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR0822466.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17347..18063
                     /gene="arcA"
                     /locus_tag="ACHURQ_000602"
     CDS             17347..18063
                     /gene="arcA"
                     /locus_tag="ACHURQ_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR0822467.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18123..19475)
                     /gene="creD"
                     /locus_tag="ACHURQ_000603"
     CDS             complement(18123..19475)
                     /gene="creD"
                     /locus_tag="ACHURQ_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR0822468.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19533..20957)
                     /gene="creC"
                     /locus_tag="ACHURQ_000604"
     CDS             complement(19533..20957)
                     /gene="creC"
                     /locus_tag="ACHURQ_000604"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR0822469.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20957..21646)
                     /gene="creB"
                     /locus_tag="ACHURQ_000605"
     CDS             complement(20957..21646)
                     /gene="creB"
                     /locus_tag="ACHURQ_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR0822470.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21659..22132)
                     /gene="creA"
                     /locus_tag="ACHURQ_000606"
     CDS             complement(21659..22132)
                     /gene="creA"
                     /locus_tag="ACHURQ_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR0822471.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22343..23212
                     /gene="robA"
                     /locus_tag="ACHURQ_000607"
     CDS             22343..23212
                     /gene="robA"
                     /locus_tag="ACHURQ_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR0822472.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="ACHURQ_000608"
     CDS             complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="ACHURQ_000608"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR0822473.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23908..24429
                     /gene="yjjX"
                     /locus_tag="ACHURQ_000609"
     CDS             23908..24429
                     /gene="yjjX"
                     /locus_tag="ACHURQ_000609"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR0822474.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="ACHURQ_000610"
     CDS             complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="ACHURQ_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR0822475.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="ACHURQ_000611"
     CDS             complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="ACHURQ_000611"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR0822476.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27078..28745
                     /gene="ettA"
                     /locus_tag="ACHURQ_000612"
     CDS             27078..28745
                     /gene="ettA"
                     /locus_tag="ACHURQ_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR0822477.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28906..29013)
                     /gene="nadS"
                     /locus_tag="ACHURQ_000613"
                     /pseudo
     CDS             complement(28906..29013)
                     /gene="nadS"
                     /locus_tag="ACHURQ_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="ACHURQ_000614"
     CDS             complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="ACHURQ_000614"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR0822478.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30305..31687)
                     /gene="radA"
                     /locus_tag="ACHURQ_000615"
     CDS             complement(30305..31687)
                     /gene="radA"
                     /locus_tag="ACHURQ_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR0822479.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31736..32704)
                     /gene="serB"
                     /locus_tag="ACHURQ_000616"
     CDS             complement(31736..32704)
                     /gene="serB"
                     /locus_tag="ACHURQ_000616"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR0822480.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32810..33454
                     /locus_tag="ACHURQ_000617"
     CDS             32810..33454
                     /locus_tag="ACHURQ_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR0822481.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33482..34498
                     /gene="lplA"
                     /locus_tag="ACHURQ_000618"
     CDS             33482..34498
                     /gene="lplA"
                     /locus_tag="ACHURQ_000618"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR0822482.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34530..34793
                     /locus_tag="ACHURQ_000619"
                     /pseudo
     CDS             34530..34793
                     /locus_tag="ACHURQ_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="ACHURQ_000620"
     CDS             complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="ACHURQ_000620"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR0822483.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="ACHURQ_000621"
     CDS             complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="ACHURQ_000621"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR0822484.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="ACHURQ_000622"
     CDS             complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="ACHURQ_000622"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR0822485.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="ACHURQ_000623"
     CDS             complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="ACHURQ_000623"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR0822486.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39514..41064
                     /locus_tag="ACHURQ_000624"
     CDS             39514..41064
                     /locus_tag="ACHURQ_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR0822487.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41036..41899
                     /locus_tag="ACHURQ_000625"
     CDS             41036..41899
                     /locus_tag="ACHURQ_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR0822488.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9945 a        11410 c        10590 g        10188 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagcg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcgatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 atattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt
     8341 ttatcggcat ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg
     8401 acgtcaatgg acagtttcgt ggaggaccgg catggtatat ggcgcgcggg ctggggatgc
     8461 gttggatggg cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg
     8521 gagttcaggc gaacgccgtt gcccgagccc tgagttttct tttgattttc ccccgctggt
     8581 gacaggcatt attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt
     8641 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtgc tgaccagcat
     8701 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
     8761 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
     8821 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa
     8881 cgcggcagcg gcagcggcgt cctggcctcc gcatccggca gcgcaaggga ttgtccagat
     8941 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
     9001 ggcgggtaac gacacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
     9061 gcgggtgcta atgggttcct ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc
     9121 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
     9181 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
     9241 caccttgcta agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
     9301 tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga
     9361 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
     9421 tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
     9481 atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga
     9541 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
     9601 gcgctatacg ctgccggagc tgttagacaa ttcccagcac ttgatccatg aagcgcggaa
     9661 actgacgcct ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa
     9721 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggctat
     9781 tttggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaactttca gcgaagacga
     9841 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc
     9901 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg
     9961 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
    10021 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
    10081 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
    10141 atttaagata atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
    10201 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    10261 tgatgaagct tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    10321 ttgcagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt
    10381 caatatattg cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg
    10441 aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    10501 gccgtgatga tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg
    10561 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    10621 acctccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg
    10681 gactaacgta atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc
    10741 gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    10801 cgatatccgg agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct
    10861 aagttaccgc atttattgag aatttctcct ttttccctgt cgtttttctc cataatttct
    10921 tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt
    10981 ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga
    11041 aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    11101 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    11161 ttcgcctgga ttcaactggt cacacagcgc acgatacgct acggcagcgt gcggctccga
    11221 ggtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    11281 tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    11341 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    11401 tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    11461 aaaacgtttc accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa
    11521 gtttccgctt ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac
    11581 tgcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagccga
    11641 gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg
    11701 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca acgtacagaa
    11761 cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt
    11821 cggcaaaccg tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag
    11881 aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    11941 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    12001 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    12061 ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    12121 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcat gcggaaaaaa
    12181 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcgtt
    12241 gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    12301 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    12361 ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg aacaaggtcg ggccggagcc
    12421 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    12481 aggcagtaac cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag
    12541 ctcaggctga cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat
    12601 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    12661 aattcccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    12721 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    12781 gtaatgaatg ctgccggaga tacgtccttc cagctcgccc atcaaagcca gcaaacgggt
    12841 gtcattaagt ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccaccgaaca
    12901 ggcgctggag cctaagcccg agccgatcgg catattcttt tccagagtca tcgccactgg
    12961 aatttgcttg cccagctcca ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    13021 tggttctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc
    13081 cgcctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc
    13141 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    13201 gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca ccagcagcgg
    13261 taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc
    13321 tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttcccatcca
    13381 cttcggcaat cttcacgcgg cagacaccat cttcatcaat attgccaaca tagcgcaaaa
    13441 cttttccttc atcacgggcc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    13501 gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc agcacaggtt
    13561 caatttcaat atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagtt
    13621 tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta taacccattt
    13681 cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    13741 aagaaagcga acctgaaaga atgccggaga acttcaccaa ttcatcacct gcattgagca
    13801 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    13861 tacgccgcga tttttccgcc gcataacgca actgatgata gtaatccatc gacgaggtgt
    13921 tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg gcatattgat
    13981 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    14041 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgtgca agttcttcct
    14101 gccagttttc cagattaagg ccatgcacac tagtgagcag agccttcgag ttggcaacgc
    14161 cgcagacacg taagtcgata tgtttattct tcagccagct ttgctgacgc ttcagttgct
    14221 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    14281 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    14341 cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc
    14401 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    14461 ccaccgagat aatggccagc cgttccatca ccgccagcgg ctccagtaag ccttctttca
    14521 gttccaggta gaactcttcc tgcattgccc gttcagcccg cacacagtcg ctttgcggaa
    14581 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    14641 cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac cattcctttc atccccgggc
    14701 cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgc
    14761 cttcatcacg actggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa
    14821 tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    14881 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat
    14941 cgggcacctg acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac
    15001 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa
    15061 gtaccaccag ttcgcctttt tcattaccgg cggtgaagcc tgccatcagc accatgtgat
    15121 cagccggaat acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt
    15181 cgaggtaatg ccccactgcc agtagttttt cgaccggatc gataacggta acgttgtgac
    15241 cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca
    15301 gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact aatgccatgc aggacatgtt
    15361 ttatttgggc aaattcctgg tcgacgaaag ttttcaattg cgccagcggg aatcccggct
    15421 gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    15481 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt
    15541 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    15601 cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    15661 gcatggttgt tacctcgtta cctttggtcg aaaaaaaaag cccgcactgt caggtgcggg
    15721 cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg
    15781 tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta atttttatct
    15841 gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa taaaatttta
    15901 atttactcat ggcaggtaac cagttcagaa gctgctatca gacactcttt ttttaatcca
    15961 cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact tactaaggcg
    16021 ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac attgccgtgt
    16081 ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc gaccagtttt atgtcatctg
    16141 ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc agttgatgtt
    16201 ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat tgatagcaat
    16261 agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc ggcacaccag
    16321 taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca
    16381 ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcggcacc agttctactg
    16441 gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca gtgaaatcga
    16501 catcgtgtaa cgattcagcc agtgttggga aaactttaat attatcaata acatcaccag
    16561 atccatgtgc gacccagcgg gtggctggct ccaggtgtgc ctgactatcg acaatccgca
    16621 gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt tctgctctgg
    16681 cgggtgcgac caggataatc gttatacgca tattgccact cttcttgacc aaataaccgc
    16741 gaacagggtg atcactgtca acttattacg cggtgcgaat ttacaaattc ttaacgtaag
    16801 tcgcagaaaa agccctttac ttagcttaaa aaaggctaaa ctatttcctg actgtactaa
    16861 cggtttaatt gttaaaaaat gctacatatc cttctgttta cttaggataa ttttataaaa
    16921 aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa ctagttggat
    16981 tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca aactgttaac
    17041 gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt gtccggcccc
    17101 tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt gacactgtcg
    17161 ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg atggaaagtg
    17221 catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa tttgcagcat
    17281 gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc aatttaggta
    17341 gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac acgcaacacg
    17401 ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga tggcgcggaa
    17461 atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat caatctgccg
    17521 ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg
    17581 ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat
    17641 gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg caacctgctg
    17701 tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga aagctacaag
    17761 ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga tggcgagcag
    17821 tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa
    17881 attcagtccc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa accacacgac
    17941 cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac gccggatacg
    18001 ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga tctggaagat
    18061 taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
    18121 ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
    18181 ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc
    18241 accaccagca cactggttcc caacaacagc gcgctatcga cagagttgag cagtccccac
    18301 atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc
    18361 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
    18421 gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
    18481 gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
    18541 acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc
    18601 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcgag gaagttttcc
    18661 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
    18721 tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggcaa aaaatcacct
    18781 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg cccggcaggc
    18841 accacagaaa gatcgccggt accgcttaaa ttcagggcca tattcagctt caggttctgc
    18901 ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
    18961 gtgccgggtt caatggtcag cgccgttccg ttaacttcag gcgctttcac cacaccaata
    19021 ccacgcgcat ccccgacgct aatcacaata aatggcttgc caaaggtgat atttggcgcg
    19081 tcgagttcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
    19141 acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc atcaaccatc
    19201 aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt tttatcctct
    19261 tcctgtaccg tataaagctc ggtcacagga atagcgataa gcggcccaac aagtttttgc
    19321 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
    19381 acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacagg
    19441 gtggtcattt tccagaacag ggaggatttc aacatagtct tctcctttgc aatggcagca
    19501 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgaagc
    19561 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
    19621 gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
    19681 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
    19741 tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt tatacgaccg
    19801 ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg atccagtaac
    19861 gccggttcag cagcaacatt aacctcggtg ggcgtaacat gtagagtgat gtttttttct
    19921 gccaactgca cggtgcgcgc ttcgctgacg cggcggaata atgccgccac atcaacagca
    19981 gtcaaaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagt
    20041 gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
    20101 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
    20161 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
    20221 atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc gggaagagga
    20281 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat ggagcggttg
    20341 atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc ccaacaaaat ggcgctggcc
    20401 cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt cggtttgcct
    20461 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcacc
    20521 gaactttcgg gatcggcagg attttgcaac gtgctacgcg caccatactg accacgcaac
    20581 gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg
    20641 aacaatactt tgccctgcgc atcggtcata tagacatgat attcattgcg cactttgtta
    20701 atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
    20761 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt
    20821 gcggtatcga ttaacgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
    20881 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc cagcagcaac
    20941 cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga tgagtattaa
    21001 tcggagaaag atcggggttg atggcgcgca gcttggcacg caacgtttta atgtgggtat
    21061 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt agttgctggc
    21121 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
    21181 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
    21241 gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc agtaaggtgc
    21301 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
    21361 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
    21421 acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata tcaggcaaac
    21481 caacatcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc accggcaagc
    21541 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
    21601 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
    21661 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
    21721 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
    21781 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
    21841 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatagccg
    21901 catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt ttcgcccggc
    21961 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
    22021 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
    22081 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
    22141 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
    22201 caaaatctca atacttttat ttccgtcaag ccctaaaaca tactctacta agaaaaaaac
    22261 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
    22321 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
    22381 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
    22441 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
    22501 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
    22561 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
    22621 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
    22681 tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat ttgtcaccct
    22741 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
    22801 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
    22861 cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
    22921 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
    22981 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
    23041 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaacct
    23101 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
    23161 agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt aacgctgcag
    23221 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa cgacccagcc
    23281 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
    23341 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac atcccagtgc
    23401 aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
    23461 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
    23521 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
    23581 atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
    23641 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
    23701 gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
    23761 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
    23821 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
    23881 acgttaattt tctgagtaat gctgattatg caccaagtta tctgtgcgac caccaatccc
    23941 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
    24001 attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag tgaggaaacg
    24061 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
    24121 tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt ggtgattgaa
    24181 aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc ggtgattctg
    24241 cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
    24301 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc
    24361 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag
    24421 gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac gatgcctgat
    24481 gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc
    24541 cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt
    24601 cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc cacgcaacag
    24661 ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag
    24721 gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg
    24781 taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat
    24841 aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta ctcgctaaag
    24901 agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc cgtggcgctc
    24961 atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc cagcacgttc
    25021 ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata
    25081 cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct
    25141 gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta actggtgcca
    25201 atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag
    25261 aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc gctggcccct
    25321 accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag
    25381 ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg
    25441 aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc
    25501 caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt tgacttgctc
    25561 ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta catcaactcg
    25621 cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg cggcgcttta
    25681 tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg gtagaaacca
    25741 cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc gcgttccagc
    25801 aataaatcca cctgccagta acgccattca tctttctctt ttgcttccat cggcagacgc
    25861 gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg cacacggcgt
    25921 tcaatcaacg acgtggattg cgagcgcata atgacgtcat cgcgccattt cgcctgctcg
    25981 tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga
    26041 tcttcattaa gctgctgcac ctgggcaagc gacgggatca tcagccgtgc attttcagca
    26101 tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa atcggtcgct
    26161 ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc
    26221 gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggccagtg
    26281 ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct
    26341 gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc
    26401 cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag
    26461 tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca
    26521 cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga
    26581 ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcaga
    26641 tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc
    26701 atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa
    26761 cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag
    26821 acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct
    26881 aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt
    26941 gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact
    27001 gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc acgataaaca
    27061 tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg
    27121 ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa
    27181 ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt atggcgggca
    27241 ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttacctgc
    27301 cgcaggagcc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt
    27361 ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg
    27421 atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc
    27481 acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg
    27541 actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta gcgttgtgcc
    27601 gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg
    27661 ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc gtggtggcga
    27721 ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg
    27781 gtgaaggtat tccgtgggaa ggcaactact cctcctggct ggagcagaaa gatcagcgcc
    27841 tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt
    27901 gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg
    27961 aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg tttattccac
    28021 ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggtg
    28081 atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc gtcgggatca
    28141 tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt caggaacagc
    28201 cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcgtcggtt gatcagttcc
    28261 gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta
    28321 tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag
    28381 gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc
    28441 tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc
    28501 tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga
    28561 tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc
    28621 aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa gagtacaaga
    28681 aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag cgtattgcga
    28741 agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca aaccaagaaa
    28801 tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc atcaggcatt
    28861 gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaacag ccttagtatc
    28921 cgcaatttcg tagcgggtca cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc
    28981 ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc ctttgagccg
    29041 tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac gcagcgcagg
    29101 aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc
    29161 atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa
    29221 ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca
    29281 tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg
    29341 gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac tgcaaaatca
    29401 atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa tgcgatctca
    29461 tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact ggtggtattg
    29521 aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc accaaggatc
    29581 gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca gtagcggaac
    29641 gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg atcgaccagc
    29701 accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag
    29761 atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca
    29821 tcccagccgt gcggatacgg ctccatgccc tcttcgttga aagcatgaat gcgaatattt
    29881 ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt gggctgctgc
    29941 gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata
    30001 tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcagc
    30061 gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa ttcaagcccc
    30121 aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt ggcattcagt
    30181 aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg tagcgtgcag
    30241 ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct
    30301 ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct
    30361 gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac
    30421 cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg atctcccctg
    30481 ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca cgcaggctgg
    30541 aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc acgccgccga
    30601 ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg tgcaagacag
    30661 ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc ggattcgcca
    30721 tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca
    30781 ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa attgccgaag
    30841 ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg
    30901 cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca tcaccatcca
    30961 gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc gagccatctt
    31021 tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag cgcgtcagat
    31081 aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca tccgccatat
    31141 gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg gcaatcaggc
    31201 agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc
    31261 gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac agcgttttca
    31321 tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc gcacctgggt
    31381 taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg cggtcgaact
    31441 ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag agtttctgga
    31501 cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc gccaccgttg
    31561 gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc
    31621 cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt
    31681 ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg agcaattact
    31741 tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg
    31801 tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc agccctgctg
    31861 ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc gccagcggga
    31921 tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg tctacgatgt
    31981 cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg
    32041 cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg ccggaggcaa
    32101 tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag cctggcatca
    32161 gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca
    32221 cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttactt
    32281 ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat tcaatctgga
    32341 tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc gggattttcc
    32401 ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt gcggtgagtg
    32461 aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatcg
    32521 ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca cgaccataca
    32581 gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg
    32641 aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag
    32701 gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt
    32761 atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac
    32821 aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg ccttgttagt
    32881 ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca
    32941 gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat
    33001 gcgtaccgac tcaccagatg aaaaacgcat tcaggcgatc ctcgatcagt taacggatga
    33061 aagccgcatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg cccgttcagg
    33121 cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcag gcggctattt
    33181 taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct atctgcgcct
    33241 gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat
    33301 tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc gcacgctgct
    33361 acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt
    33421 gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt
    33481 tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt
    33541 ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa
    33601 tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg
    33661 gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg cggtgttcca
    33721 cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaaccat
    33781 ctctacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg
    33841 acgtaacgat ctggtggtga aaacagtcga aggcgaccgc aaagtctcag gctcggccta
    33901 tcgcgaaacc aaagatcgtg gcttccacca cggcactttg ctgctcaatg ccgaccttag
    33961 ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag gcattacctc
    34021 agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc atgagcaggt
    34081 ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag aagcggaaat
    34141 catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg cccgtcagag
    34201 tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg atgaacgctt
    34261 tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca cccgcgccca
    34321 ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc gactgcaagg
    34381 ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc
    34441 ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg taaggtaact
    34501 cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag gagcgggaga
    34561 ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt atcaggcata
    34621 aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct ctaaaataga
    34681 tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatgggagaa atttatggtt
    34741 gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa tgaatagatt
    34801 cgtgaataga tacttgaatg tattttattt tttatccata tgaaaaataa tagtaaatct
    34861 ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc ccactccggc
    34921 ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca gcagaacgga
    34981 ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg
    35041 ctcgtgagtg cggatgtggt cagacacagt gcagatggtc agggctttcg cgccaaattc
    35101 tgcagcgacg ccgtagatac cagccgcttc catttccacg ccgaggatgc cgtatttttc
    35161 catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga acaggttacc
    35221 aacgcgagca tcaatgccca gtgctttagc cgcgtctacc gcgttacgca ccatgtcaaa
    35281 gtcagcgata gcggcaaagt catgatcttt aaaacggatg cggttaactt tggaatcggt
    35341 gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa ctgcgccaca
    35401 ggaacccacg cggataattt tcttcacgcc gaaatcggtg atcagttctt tggtgtagat
    35461 ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc ctttgtaagt
    35521 accggtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt
    35581 ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc
    35641 gcccatttct gcattaatgt gtggggtagc cattgtttta tccttatcaa catgttttca
    35701 ccctctccct gagggagagg gccggggtga gggcatcaag ccgcacccat tatcagaaca
    35761 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    35821 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggccg taaaccagta
    35881 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcacaa ccgtggtcag
    35941 cagtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    36001 cgaacagctc cagacccaca gcataaccgg cgacgtcgcg acggtggccc caggaagagt
    36061 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    36121 tggtggcgtc aaacagcgcg tccaggccag ttgctttcac tttcttggtg ataccgcagt
    36181 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg accgtgtttt tcatcaacca
    36241 gtttctgcag cacggtcggt gctggcggct caacagccag gtcgtgacgg ttaccggtac
    36301 gctggaagtt accggctttg tcaccgataa acggacgagc gataacacga ccgatgttgt
    36361 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccaggc
    36421 caaaggtttc ttcgtggcag gcaatctgga acacagagtc agcggaggta tagaaaatcg
    36481 gcttgccggt tttcatgtgc tcttcgccca gctgatccag aatgaccgta ccggaagagt
    36541 ggcagttacc gaggtaaccc ggcagattag cgcgttcaac cagtttatcc agcagctctt
    36601 gcgggaagct gttttcgtga tcggagaaat atccccactc aaatagaacc gggacaccgg
    36661 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    36721 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    36781 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcgga cctttacgac
    36841 cgttatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    36901 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    36961 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    37021 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gctttatcgg
    37081 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    37141 cgtggataac cgccagcgga cgctgaccgt ctacctggtc gcccaaacgc gccatatcag
    37201 taaagccgac gctgtaatca atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    37261 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    37321 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    37381 ttgggccttt ttgtgccgct accatacgac caaagacttc tgctgcttta ccgttgtcca
    37441 gcactgcttg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagataa
    37501 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    37561 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    37621 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    37681 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    37741 tcacgtccat caccagcgcg tccagacctt ccgcaagttt cttcgccaga atagaggcgg
    37801 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    37861 ccgccggagc cagtgagctg gtctgaccga taatcgccac gccgacgtct ttaataattt
    37921 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    37981 gcgtaccgcc agtatgaccg aggccgcgac cagagatcat cggaatatag ccgccgcagg
    38041 ctgcgaccat cggccccaac atcagcgaag tcacatcgcc gacgccacca gtggaatgtt
    38101 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    38161 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    38221 tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc gcgaatgccg ttgataaaga
    38281 aacgaatttc ttcatcgctc agtgcatgac catcacgttt tttacgaata atttcttgtg
    38341 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    38401 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    38461 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcaccgtgg cccagcgctt
    38521 tcaacaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tctgcccagt
    38581 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    38641 cgccgcccgc tggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    38701 tcatgatgcg cgcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    38761 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    38821 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    38881 tacaggcttt caccaggtca aaaccaacct gctcgttacc cgccatcagc gcgcggtacg
    38941 ggaacacaac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    39001 cgatttcgat gtcatcgtta ccgtgtggga agttggttac cgtagcgatt cggatttccg
    39061 gggtgccctg ctctttcagc gttttgcgag caatcgggat aaagcgagga tagatacaga
    39121 tagcggcggt attgccgacc ggggttttgg cctgatgaca cagagcaatt actttctcgt
    39181 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    39241 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    39301 tgttagtatt ctaacatcta ctccgcaaca cacttcgata tacatcacaa ttaaggaaat
    39361 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    39421 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    39481 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    39541 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    39601 tttctggcac tggaagcaga aaacaatctg ccttatccgc agcttcctgc cgaagcccgc
    39661 cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    39721 cgctatgtct tacccgatta cgcccgtttt ctggcgaacg gttccgaatg gctggagctg
    39781 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    39841 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccatat
    39901 gttagaattc taacacaaga cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    39961 gacagaaccc tgccagacgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    40021 acccgcgcga tcttacgtgc agacgcagag ttgaagcagg tttcaccgaa cctgaccttt
    40081 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    40141 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    40201 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    40261 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    40321 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc
    40381 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    40441 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    40501 tgtaagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    40561 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    40621 caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg
    40681 ggcgcgcgtt tgccgtatgg tgatgagcca gatccgatca cccatctgca aactgtcgcc
    40741 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc
    40801 aaacgtaatc cgcaggcgct ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    40861 gaatttactg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    40921 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    40981 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    41041 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    41101 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    41161 gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    41221 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtt tgcgagcagt gtgatacctg
    41281 cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa tccatgagcg tggacgaagt
    41341 gcttagccat gtccgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    41401 agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    41461 actgcgccac ctcacctgcc tggtggacag taacggcatg ttgagcgaaa ccggctggga
    41521 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcgtggg ggagcgaatg
    41581 tcatcaacac ttaaccggac gcgataatca gcagattaag cgcagcatct gtttgcttgc
    41641 agagcgcggc aagctggcgg aactgcgttt gctggtaatt ccagaccagg tggattattt
    41701 gcatcacatc gatgagctgg cgacgtttat caagagactt ggcgatgttc cggttcgcct
    41761 gaatgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    41821 ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc ggggtgagcc ggttgatatt
    41881 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    41941 atgataagac gcgacaagcg tcgcatcagg cattatgcgc cgactgccgg atgcggcgtg
    42001 aacgccttat ccggcctacg gttcggcaca gacttgtagg catgataaga cgcgtcagcg
    42061 tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg aacgccttat ccggcctacg
    42121 gttcggcaca gac
//
LOCUS       DAZOZY010000013        40702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZOZY010000013 DAZOZY010000000
VERSION     DAZOZY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.013"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            245..1342
                     /gene="mltA"
                     /locus_tag="ACHURQ_000626"
     CDS             245..1342
                     /gene="mltA"
                     /locus_tag="ACHURQ_000626"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR0822489.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1419..2225
                     /gene="tcdA"
                     /locus_tag="ACHURQ_000627"
     CDS             1419..2225
                     /gene="tcdA"
                     /locus_tag="ACHURQ_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR0822490.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2276..2719)
                     /gene="csdE"
                     /locus_tag="ACHURQ_000628"
     CDS             complement(2276..2719)
                     /gene="csdE"
                     /locus_tag="ACHURQ_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR0822491.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2719..3924)
                     /gene="csdA"
                     /locus_tag="ACHURQ_000629"
     CDS             complement(2719..3924)
                     /gene="csdA"
                     /locus_tag="ACHURQ_000629"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR0822492.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4116..4343
                     /locus_tag="ACHURQ_000630"
     CDS             4116..4343
                     /locus_tag="ACHURQ_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR0822493.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4694..5611
                     /gene="gcvA"
                     /locus_tag="ACHURQ_000631"
     CDS             4694..5611
                     /gene="gcvA"
                     /locus_tag="ACHURQ_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR0822494.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5630..6025
                     /locus_tag="ACHURQ_000632"
     CDS             5630..6025
                     /locus_tag="ACHURQ_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR0822495.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            6018..7118
                     /gene="rlmM"
                     /locus_tag="ACHURQ_000633"
     CDS             6018..7118
                     /gene="rlmM"
                     /locus_tag="ACHURQ_000633"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR0822496.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7162..7893)
                     /gene="fucR"
                     /locus_tag="ACHURQ_000634"
     CDS             complement(7162..7893)
                     /gene="fucR"
                     /locus_tag="ACHURQ_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR0822497.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7951..8373)
                     /gene="fucU"
                     /locus_tag="ACHURQ_000635"
     CDS             complement(7951..8373)
                     /gene="fucU"
                     /locus_tag="ACHURQ_000635"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR0822498.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8375..9793)
                     /gene="fucK"
                     /locus_tag="ACHURQ_000636"
     CDS             complement(8375..9793)
                     /gene="fucK"
                     /locus_tag="ACHURQ_000636"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR0822499.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9902..11677)
                     /gene="fucI"
                     /locus_tag="ACHURQ_000637"
     CDS             complement(9902..11677)
                     /gene="fucI"
                     /locus_tag="ACHURQ_000637"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR0822500.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11710..13026)
                     /gene="fucP"
                     /locus_tag="ACHURQ_000638"
     CDS             complement(11710..13026)
                     /gene="fucP"
                     /locus_tag="ACHURQ_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR0822501.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            13573..14220
                     /gene="fucA"
                     /locus_tag="ACHURQ_000639"
     CDS             13573..14220
                     /gene="fucA"
                     /locus_tag="ACHURQ_000639"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR0822502.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14248..15396
                     /gene="fucO"
                     /locus_tag="ACHURQ_000640"
     CDS             14248..15396
                     /gene="fucO"
                     /locus_tag="ACHURQ_000640"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR0822503.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15501..16256)
                     /gene="xni"
                     /locus_tag="ACHURQ_000641"
     CDS             complement(15501..16256)
                     /gene="xni"
                     /locus_tag="ACHURQ_000641"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR0822504.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16368..17735)
                     /gene="sdaB"
                     /locus_tag="ACHURQ_000642"
     CDS             complement(16368..17735)
                     /gene="sdaB"
                     /locus_tag="ACHURQ_000642"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR0822505.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17793..19082)
                     /gene="sdaC"
                     /locus_tag="ACHURQ_000643"
     CDS             complement(17793..19082)
                     /gene="sdaC"
                     /locus_tag="ACHURQ_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR0822506.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19638..21002)
                     /gene="ppnN"
                     /locus_tag="ACHURQ_000644"
     CDS             complement(19638..21002)
                     /gene="ppnN"
                     /locus_tag="ACHURQ_000644"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR0822507.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21114..21962)
                     /gene="queF"
                     /locus_tag="ACHURQ_000645"
     CDS             complement(21114..21962)
                     /gene="queF"
                     /locus_tag="ACHURQ_000645"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR0822508.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22030..22575
                     /gene="syd"
                     /locus_tag="ACHURQ_000646"
     CDS             22030..22575
                     /gene="syd"
                     /locus_tag="ACHURQ_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR0822509.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23197..23526
                     /locus_tag="ACHURQ_000647"
     CDS             23197..23526
                     /locus_tag="ACHURQ_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR0822510.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23526..24308
                     /gene="truC"
                     /locus_tag="ACHURQ_000648"
     CDS             23526..24308
                     /gene="truC"
                     /locus_tag="ACHURQ_000648"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR0822511.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24326..24775
                     /locus_tag="ACHURQ_000649"
     CDS             24326..24775
                     /locus_tag="ACHURQ_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR0822512.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25210..26562
                     /gene="gudP"
                     /locus_tag="ACHURQ_000650"
     CDS             25210..26562
                     /gene="gudP"
                     /locus_tag="ACHURQ_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR0822513.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26564..27904
                     /locus_tag="ACHURQ_000651"
     CDS             26564..27904
                     /locus_tag="ACHURQ_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR0822514.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27925..29265
                     /gene="gudD"
                     /locus_tag="ACHURQ_000652"
     CDS             27925..29265
                     /gene="gudD"
                     /locus_tag="ACHURQ_000652"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR0822515.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29497..32253)
                     /gene="barA"
                     /locus_tag="ACHURQ_000653"
     CDS             complement(29497..32253)
                     /gene="barA"
                     /locus_tag="ACHURQ_000653"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR0822516.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32310..33611
                     /gene="rlmD"
                     /locus_tag="ACHURQ_000654"
     CDS             32310..33611
                     /gene="rlmD"
                     /locus_tag="ACHURQ_000654"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR0822517.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33659..35893
                     /gene="relA"
                     /locus_tag="ACHURQ_000655"
     CDS             33659..35893
                     /gene="relA"
                     /locus_tag="ACHURQ_000655"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR0822518.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35971..36219
                     /gene="mazE"
                     /locus_tag="ACHURQ_000656"
     CDS             35971..36219
                     /gene="mazE"
                     /locus_tag="ACHURQ_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR0822519.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36219..36554
                     /gene="mazF"
                     /locus_tag="ACHURQ_000657"
     CDS             36219..36554
                     /gene="mazF"
                     /locus_tag="ACHURQ_000657"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR0822520.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36625..37416
                     /gene="mazG"
                     /locus_tag="ACHURQ_000658"
     CDS             36625..37416
                     /gene="mazG"
                     /locus_tag="ACHURQ_000658"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR0822521.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37644..39281
                     /gene="pyrG"
                     /locus_tag="ACHURQ_000659"
     CDS             37644..39281
                     /gene="pyrG"
                     /locus_tag="ACHURQ_000659"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR0822522.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39369..40667
                     /gene="eno"
                     /locus_tag="ACHURQ_000660"
     CDS             39369..40667
                     /gene="eno"
                     /locus_tag="ACHURQ_000660"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR0822523.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
BASE COUNT        10076 a         9859 c        10827 g         9940 t
ORIGIN      
        1 tgagcccgac gagctaccag gctgctccac cccgcctccg tggatgcgca ctatactccg
       61 ctcgcgtttt catgcaacct tttttttacc ccgatcacgg atctggcatg attttgaaca
      121 aaaacaaatc actaaagcct attttttgtg caaaatttgc gtcagggaga ggctgctcat
      181 ttcattaaac gcggcggttt gttatcttcg ttgcgcctta ttttttaacc tgaagaagag
      241 aacaatgaaa ggacgttggg taaagtacct tcttatgggc acggttgtgg caatgcttgc
      301 cgcctgctct tccaaaccaa ccgatcgcgg acagcaatat aaagacggga aatttaccca
      361 gcctttctct ctggtgaacc agccagatgc cgttggcgcg ccgattaacg ccggtgattt
      421 tgccgagcaa attaaccata tccgtaattc gtcaccgcgt ctgtatggca accagagtaa
      481 tgtttataac gcggtgcaag agtggctgcg cgcaggcggt gatacccgca atatgcgcca
      541 gttcggcatt gatgcctggc agatggaagg tgccgacaac tatggtaacg tgcagtttac
      601 aggctattac acgccggtaa ttcaggcgcg ccatacccgc cagggcgagt tccagtatcc
      661 tatttaccgt atgccgccaa aacgtggtcg tctgccgtct cgtgcggaga tctacgcggg
      721 ggcattgagt gataaatata ttctcgctta cagtaactcc ctgatggata acttcattat
      781 ggatgtgcag ggtagtgggt atatcgactt tggtgatggc agtccgctta actttttcag
      841 ctatgcaggg aaaaacggtc atgcctatcg cagcattggt aaggtgctga tcgaccgtgg
      901 cgaagtgaaa aaagaagata tgtcgatgca ggcgattcgt cactggggcg aaacacacag
      961 tgaagccgag gttcgcgagc tgctggaaca gaacccgtct ttcgtcttct ttaaaccgca
     1021 atcttttgca ccggtgaaag gggcaagtgc ggtgccgctg gttggtcgcg cgtcagttgc
     1081 ctctgatcgt tccattattc cgccaggtac taccttgctg gcagaagtgc cgttgctgga
     1141 taataacggc aaatttaatg gtcagtacga actgcgtctg atggtggcgc tggatgtcgg
     1201 tggtgcaatc aaaggccaac acttcgatat ctatcaaggg atcgggccgg aagccggaca
     1261 ccgcgcaggt tggtacaacc actatggacg tgtctgggtg ctgaaaaccg ccccgggcgc
     1321 aggtaacgtc tttagcggct gatgtgttat tctgtatacc gtttttacgg atacgacagg
     1381 gtgagggata acctcaccct ttttaattct gaggtgctat gtctgtggta attagtgatg
     1441 catggcgtca gcgttttggt ggcacagcgc gtctgtatgg tgaaaaagcg ttgcaactgt
     1501 ttgctgacgc gcatatttgt gtggttggta tcggtggtgt cggttcctgg gcggcggaag
     1561 cgctggcgcg cacggggatt ggcgcaatca cgcttatcga tatggatgat gtgtgcgtca
     1621 ccaataccaa tcggcaaatt cacgctctgc gcgataacgt cgggctggca aaagcggaag
     1681 tcatggcgga gcgtattcgc cagattaacc cggagtgccg tgtaacggtg gtggatgatt
     1741 tcgtgacgcc ggataacgta gcgcagtata tgagcgtggg ttattcgtac gtgattgatg
     1801 ccattgatag tgtacggccc aaagcggcgc tgattgctta ttgtcggcgc aataaaatcc
     1861 cgctggtcac gaccggtggc gcgggtgggc agattgaccc gacgcagatt caggttaccg
     1921 atctggcgaa aacgattcag gatccgctgg cggcgaagtt gcgcgagcgc ctgaaaagcg
     1981 attttggcgt agtgaaaaac agtaaaggta agctcggcgt cgattgcgtg ttttctactg
     2041 aagcgctggt gtacccgcag tcagacggta ctgtgtgtgc gatgaaagcc acggcagaag
     2101 ggccgaagcg gatggattgt gcatctggat ttggcgcggc aacgatggtg accgccacct
     2161 ttgggtttgt tgcggtttct catgcgctga agaagatgat ggcgaaagcg gcgcgtcagg
     2221 gttaaacaat caggtcggat gagacgcgaa cgtcttatcc gacccggttc tatgcttaaa
     2281 cctgcttcgt cgcagcgata atcgcctcgc ttaacgcatt taacccctgg ctgcgtgagg
     2341 cgctaagttg cgcacgcaat cccagctcat caaacaatgc cagcggcgac tgtgcctgca
     2401 attcggcggc ggttttcccc tcaacggcag tcaacagcac cgccagcagg ccgcgcacaa
     2461 tgcgcccttc gctatcgcca aagaagtgca ttttaccgtt ttcagtcacg gtatatccca
     2521 gccagacgcg gttttcgcat ccggcaatct ctttagcctg cgcttttaac tcgtctggca
     2581 aagccggaag ctgtttcccc agcatgatca actggcgata tttatcttcc cattgcgaca
     2641 acggtgcgaa ggtattgcgt aacgtttctg cggttacggt tgtgccgaac ggatgtccgg
     2701 cgaattgcgg gttcgtcatt aatccaccaa taattccagc gcgcggtcaa cggcattcac
     2761 cagcgcatcc acatcactct ttgtattata tggcgcaaaa gaggcgcgca gtgtgccggt
     2821 tacgcctaat tctgccagta gcggctgagc gcaatgttgc ccggcccgca gggcaatacc
     2881 gtactccgcc agcagcgtca ccatatcgct gtgatgaacg ccagcaaaat caaaggccag
     2941 caggctggaa tcctggcagc ggaatgagcg gaagccagga cgttttgcca gcgcatcttc
     3001 cgccagcgtc gccaggctgc gactccaact ttctgcctgg ttgatatcgt aatctgccag
     3061 ccattccagc gccgcgctta atcctatgac accagccaca tttggcgttc ctgcttccag
     3121 tttccacggc gcagattgag tcgtgaagcc gtcaaaactc acttcgtgaa tcattttgcc
     3181 gccacccagc cagggcgaca tcgcttccag cagttctgat ttgccataca gcacgccgat
     3241 acccgtcggg ccatacagtt tgtgaccaga gaaagcatag aaatcgatat ctagttgctg
     3301 aacatctgcg gggaaatgca ccgccccctg agcaccatca accatcacca ccatcccggc
     3361 tgaatgagca aaggtaatcg ctcgcgccag atccgggcaa ccgccagtaa cgttcgacat
     3421 ctgacccaac gccagaatcc gactacgggg agtaatcagt tctggcaaca aatcgacatc
     3481 cggcagtcgc tgcgcattaa gcggcaattt caccactttg gctccagttt gttgggcgac
     3541 catcagccag gggacgaggt tagcgtggtg ttctgccacg ctgacaataa tctcatcgcc
     3601 cggttgcaga cgcggacgcg catagcattg tgccaccatg ttgatggatt cagtggtgcc
     3661 gcgcgtccag acgatagttt tatcatccgg tgcattcagt aattgcgcca ctttctctcg
     3721 tgcagcttca taacgcgcgg tcaggcgttg ggcttcggca aactggctgc gatggacgtt
     3781 tccggcgctc aggctgtaaa actgtcgagt ggcttcaacc acggcttcag gtttaagcgc
     3841 ggtcgcggcg ctatcgagat agacgcccgc atcctgtagt gcgggaaact gggcgcgaaa
     3901 ctgcgcggga ttaaaaacgt tcatggtact cctcggcttg atgcaccgat cgtgacgcaa
     3961 aattcacttt gatacaagga gagtgctaac catcagaatt atctggatat cctggcgaag
     4021 ccgcgtaagt aacatatgct gaatagtgtt aggcgttaat tttagcctgt taatcaatac
     4081 tattaagatt tattaatcgc tttaaggaga agatgatgaa aaagactgcc gcaattattt
     4141 ctgcctgtat gctgactttt gccctgagcg cctgttccgg ttcgaactat gtgatgcaca
     4201 ccaatgacgg acgtaccatc gtctctgacg gcaaaccaca gactgataac gataccggta
     4261 tgatttcgta taaagacgct aatggcaaca aacagcagat caaccgtact gacgtgaaag
     4321 agatggtcga actggatcag taattcgcga tcgcaaggta aaaaaaagca ccgcaattag
     4381 gcggtgctac attaatcact atggacagac agggtaaatg tacaggaagt gaaaaaaggt
     4441 agctttgcta ccatggtctg aatcgcagac caattgcaaa cacaacaaca caacatcaca
     4501 accgtaagcc aaaagttcac cagaacacgc attccgataa aacttttcgt tccggctcag
     4561 gaagtgccgc cactataggt atttgctggt agaagctcaa cggacaattt ataatggctc
     4621 agattaaaaa aactaatagg ttacacagtg tgatctaatt gttaaattaa tttaacatca
     4681 aagtttaaaa gccatgtcta aacgattacc accgctaaat gccttacgag tttttgatgc
     4741 cgcagcacgc catttaagtt tcactcgcgc agcagaagag cttttcgtga cccaggccgc
     4801 agtaagtcat caaatcaagt ctcttgagga ttttttgggg ctaaaactgt tccgccgccg
     4861 taatcgttca ctcctgctga ccgaggaagg gcaaagctat ttcctcgata tcaaagagat
     4921 attttcgcaa ttaaccgaag cgacgcgtaa actccaggcc cgtagcgcca agggggcgtt
     4981 gacggtcagt ttactcccca gtttcgccat tcattggttg gttccgcgac tttccagctt
     5041 taattcagct tatccgggaa ttgacgttcg aatccaggcg gttgatcgtc aggaagataa
     5101 gctggcggat gatgttgatg tggcgatatt ttatggtcgg ggcaactggc cggggctacg
     5161 ggtggaaaaa ctgtacgccg aatatttatt gccggtgtgt tcgccgctac tgctgacagg
     5221 cgaaaaaccc ttgaagaccc cggaagatct ggctaaacat acgttattac atgatgcgtc
     5281 acgccgtgac tggcagacat atacccgaca gttggggtta aatcatatca acgttcagca
     5341 agggccaatt tttagtcata gcgccatggt gctgcaagcg gctattcacg ggcagggagt
     5401 ggcgctggca aataacgtga tggcgcaatc tgaaatcgag gccggacgtc ttgtttgccc
     5461 gtttaatgat gttctggtca gtaaaaacgc tttttatctg gtttgtcatg acagccaggc
     5521 agaactgggt aaaatagccg cctttcgcca atggatcctg gcgaaagccg ctgctgaaca
     5581 agaaaaattc cgctttcgtt atgaacaata atttacgtag ggtacgacca tgaccagccg
     5641 ttttatgctg attttcgccg ccattagcgg cttcattttt gtggctctgg gcgcttttgg
     5701 cgcgcatgtg ttaagtaaaa ccatgggggc cgttgagatg ggctggatcc agaccggcct
     5761 cgaataccag gcgtttcata cgctggcgat cttaggtctg gcggtggcaa tgcagcgtcg
     5821 catcagtatc tggttttact ggagtagcgt tttcctcgcg ttaggcacgg tgttgttcag
     5881 cggcagcctt tattgcctgg cgctgtccca tctgcgtttg tgggcatttg tcacaccggt
     5941 tggcggcgtg agcttcctcg cgggctgggc gttaatgtta gttggtgcta tccgtttaaa
     6001 gtgcaagggc gtaagtcatg aataaggttg tattgctgtg ccgtccgggc tttgaaaaag
     6061 agtgcgccgc agaaattacc gataaagccg gccagcggga aattttcggt tttgcccgcg
     6121 tgaaagagaa tgcgggttat gtcatttatg aatgttatca acctgatgat ggcgataagt
     6181 taatccgtga gctgccgttc agttcattaa tttttgcccg ccagtggttt gtggtggggg
     6241 aactcttgca gcatttgccg ccagaagatc gtattacccc cattgtcgac atgttgcagg
     6301 gcgtagtaga gaagggcggt gaactgcgtg ttgaagttgc cgataccaac gaaagcaaag
     6361 agttgctgaa attctgccgt aaattcaccg tgccgctacg cgctgccttg cgcgatgccg
     6421 gggtgctggc gaactatgaa acgccgaagc gtccggttgt gcatgtattc tttattgcac
     6481 caggctgctg ctataccggt tactcataca gcaacaataa ttcgccgttc tatatgggca
     6541 ttccgcgcct gaaatttccg gcagaggcgc cgagtcgttc cacgctcaaa ctggaagagg
     6601 catttcatgt gtttattcct gcggatgagt gggatgaacg cctggcgaac gggatgtggg
     6661 cggtggattt aggcgcttgc cctggcggct ggacctacca actggtgaag cgcaacatgt
     6721 gggtttattc cgtcgacaac ggcccgatgg cgcaaagtct gatggatacc gggcaggtga
     6781 cgtggctgcg ggaagacggt ttcaaattcc gtccgacacg cagcaatatc tcctggatgg
     6841 tatgcgatat ggttgaaaaa ccggctaaag ttgcggcatt gatggcgcag tggctggtta
     6901 atggctggtg ccgtgaaacc attttcaatc tcaaactgcc gatgaaaaaa cgctacgaag
     6961 aagtatcaca caatctggcg tatattcagg cacagcttga tgaacatggc ataaatgctc
     7021 agattcaggc gcggcagttg tatcacgatc gcgaagaagt gacggtgcac gtccgccgca
     7081 tctgggctgc ggtgggtggt cgtcgcgacg agcgataaca ggcaaggccg gggcgtcgtc
     7141 ttcggcctgc taccctttcc gtcaggctgt taccaaagaa gttgcaacct ggcgctcatc
     7201 agaaataatg tgcgttacct catccagatg cccgatgcgg gcttcccctg aacgattaaa
     7261 tttactttta tcaatcaata acaacgattg cgcggcacgt tttaatagca tcgatttgta
     7321 atcagcgttg atcgcattgg agtcccacag tgcgccgctg ctatcgatcc cttcacaaga
     7381 aaaaataaac agatcgattt ccagcgattt aagttgggaa atcagcgagg gattgacgta
     7441 acagccatat ttgcgctcaa gtgtgccgcc ggaactgatc agttgaatgc gttcgcgttt
     7501 accgagttca tggcaaatcg gatggctatt ggtgaagacc tgaatgttga tgtcaggcaa
     7561 ctggcgtgcc agataccagc aagttgaact ggcgtctaag gctatcacca tcccttcttc
     7621 aatccacgcg agcgcctcgc gcgcgatatc tgctttatgc gcataatggc ttttcagcct
     7681 gatgtgaaag ggatcgccac tgtcttgatt ttgacggtgg atatatttag cgcgtccatg
     7741 attgcgcaga attttaccct gcgtctgtaa ttcattgaga tcgcgacgaa tggtttcttt
     7801 actgaccttt agctgttcag agagagcttc cgtggtcagg ctggtatggt tcagcagcag
     7861 gtcgactatc gcttgctggc gtgccgcttt catatttttc accccatcat tcgataaccg
     7921 cccgcgtcat gcgggcgtac cgatatgaga ttacggtgtt accccttttt ttaaaagaat
     7981 attcccgtac ttcgctcgtt cgcctgtgat aacgatcgca aaggcttttt gcgcccgttc
     8041 ataaaacgca aaacgattga tgcggatgat gtcaggacac ggggcttgta gtgaaagcgc
     8101 attacggtaa cgtcgttcta cttcaggatc gagagtgtca ccttctaccg ctgccatcat
     8161 caccagcggc ggggcataac tgtccagctc aaataatggg ataatcgctt ggagcaagtc
     8221 gctcaccaac aggccatcag cgcggatcac ctgcggtccc atcgaatggg cgggaaagtg
     8281 agcatcggaa aaaataattt catctccatg tcccatctct gccagcactt tcaatagttc
     8341 gggagaaatt aacggcgaaa ttgttttcag catttcacac ttcctctata aattcaggtt
     8401 cagtttgcgg gtagaaataa cggtactgat aatgaatctg tgcacgggct tcttccgggc
     8461 tgttaaattc ccctacgcca taccaaccga acagcgcagc tcctgcgacg gtcgtttcgg
     8521 catcgtcgag aacttttacc ggaatatcaa gcatattggc tttaatctga ttccacaatg
     8581 tgttgcgact tcctccaccg actaacaata attcagaggc tttaaagtgc ccgatttttt
     8641 ccagcatctg tagattgcgc tgtaattgcg cagttaaccc ttccagcgcc gcgcgataga
     8701 aatgcccccg cgtggtatta agcgtcactc cttgccagcc agcgttctga cacgacaata
     8761 aatcacactg cattttcaca ccatccgcgc caggtgcgat cagacgagct tcttcaatca
     8821 acatttgcca gggtgtttca gccgtccaga acagttttct cacccattcc agcacgccgg
     8881 atgccagcca ttgcatacct gggttataca accctgcctg gctatccagt tcgcaggtag
     8941 aatcggcgta ctgacttaac agcgaagtat caacctgggc gctacggacc attaaaattt
     9001 cccatgtacc ggaagagagc acgggttcat tttggtcggc accagcgcca aaaagggcga
     9061 actgggtatc gtgacctgcg gaaatcaccg gtatgccaac gggtaagccg agcattgctg
     9121 cggcgctgtt ctgtagcgta ccaatctgtt cacccgcttc caccagacga gggaagagtc
     9181 ggcgtggaat accggtggct tgtaaaattt gcggactgaa atcgcgttgc tggatatcca
     9241 gcatctggct ggttccggcc atcgtgatat cagtagtgaa ttcgccggtt aaacggtggt
     9301 taatcagcga cgaaataaag agccaggcgt gcgcgcgttc cagcagttgt ggatgatttt
     9361 ctttcaacca caccaactta tataacgtat tgaaactaaa ggctccgacg ccagaaatag
     9421 cctgcaaccg ctgtgcggag atcaactgtt caatattgtc cataaccgct gctgttcgcg
     9481 gacatttcca gctaatgatc ggatagagca gattgccttg cttatctacc agagcgccat
     9541 ccacaccaaa ggtggtgacg gcgataccgc ggatgtggca ttcagtcagt tcactattga
     9601 tttgccgaca gcaatcagca aagcgttgca aaatggcgtc taaagaccac tggtgccagg
     9661 tgttgttttc catcgcgata tcgctggcat taggcgttga ggcgcgggca acaatttttc
     9721 cctgccgatt aaccgcgatg gccctgacat tggtcgcgcc acagtcgagt accaggataa
     9781 cttcttgttt cataatcgct cctgcaatat agccggataa cattgcttat ccggctaacc
     9841 actcttgctt gttcaggccc ggtagccgga gcgaccgggc atcacatcag ggagtaatgt
     9901 attaacgctt gtacaacgga ccgtagttct ggcaagcgcg gtaatcctgg ccttcaatat
     9961 ccatgccgtg cgcagcccag gcagaaggac gatacacttt ggtctcttca acgttgtgca
    10021 tacataccgg gatacgcagc atggaggcga gagtgataaa gtctgcgcca acgtggccga
    10081 tggtcagaac cccatggtta gcgccccagt tcgccattac cgagtacaca tccgtaaacg
    10141 gccctttacc ggtgaggcgc ggtgcaaacc aggtggttgg ccaggttgag ttggtgcgtt
    10201 tgttgaggat gtcatgcaca tccttcggca attccacgct ccagccttcc gcgatttgca
    10261 gtaccgatcc caggcctttg atgatgttga cacgagtcat ggtgaacggg acgccgcctt
    10321 cggtaaggaa gcgggaagag taaccgccgc cacggaagta ttcgtggatc gccgggcacc
    10381 attcggtagc ggcgaggcaa gcgtcagcct cttgctgaga gatttcccag tgtggcttca
    10441 tcgtcgggtt accttcgctg tcgcgttgtt tacaggaacc gtccagcgca gcagaacctg
    10501 agttgatcaa atggatgatg ccgtgttctg ccagtccatc gagtttatgc cccgttacac
    10561 gctcaattgc ttctggtgac cagtaggtac gcacatcggc aaatacctga gcggtgccgg
    10621 tgagctggtg acccattagc attgccacgc cgttaagact gtcgttttcg gtcgccacga
    10681 caaagggttc gcgcacgcca ttccagtcaa atgaactgtt gaggatcgct tcggcggtgt
    10741 caccattggg atattgatcg gtccagtgac gttgcccctg gaagcccgca gcgatggcgt
    10801 tgtagccaag tgattcttcc acgcgaccaa tatcggccag tttgctgttg ccttgcatca
    10861 tgtcgcggat acacatcgcc atcagtaaac tttcgcgcag aactgcgcgg ctttgctcgg
    10921 cattgcgttg atactgtttg ttattttcat cttcgccata gcggaagttt ttatcagccc
    10981 aggtcagtgc catttccagt tcggcttcgt cgtaaatctt ctgatcgata cggcgacgca
    11041 gttcggtcat atccaccgcc tggactttca ttcccagcca ggattcaaag aagttgtgat
    11101 caacaataga accggcgata cccatcgaaa cgccgcccag cgacagatag cttttacctt
    11161 tcatgctggc gacggccaaa ccggcgcggg caaagcgcag cagtttttct tcaacatcgg
    11221 caggaatcga tgtgtcatcg gcatcctgaa cgtcatgacc gtaaatggag aatgccggga
    11281 tgcctttctg gctgtgagct gccagagccg ctgccaggta aacagcgccg ggacgttcag
    11341 tgccgttaaa gccccaaatg gctttcgggc gggttggatc catgtcgatg gtttcactgc
    11401 catagcacca gcaaggcgtt accgtaatgg tgaggcctac attctggctg ctgaattttt
    11461 cttcgcaagc agcggcttca gccatacccg cgatacaggt atcggaaatg acacactcga
    11521 cggcagctcc gcaggcatgg cgcagtttct cggtcagcag tgcggccgta gctttcgcca
    11581 tattcattgt ttgttcttca agcgactcac gaacacccat gcgacgaccg tcaataaccg
    11641 ggcggatacc aattttcggt aagctgattt ttttcattac agattcctca ctttattcgg
    11701 aaaatatgtt cagttagttg ccgtttgaga gcggaaacgg gcaaagataa agatgaccgc
    11761 gaagcagagt gtggggatca gttcagcagt ggggatgttg cccgccgcgt cactgacaaa
    11821 acccatgacc ggagtgacaa taccgccgcc aataatggtc ataacgatga aggacgaacc
    11881 gtatttggtg tcctggccga gattcttaat gcccagcgag aagattgttg ggtactgaat
    11941 cgacataaag gcgctgcata aagtcagggc tattaagccc acatgaccgc cagcgaaggc
    12001 tgagatcagg cacagtgcca tagcgattaa tgcgtaggcg gccaggactt tgtgtggtgc
    12061 gaagcgactg atgagccagg taccggtgaa acgaccaata aagaagcaca ccatggttcc
    12121 ggttaaatag ttagcggcaa agcctgcagt catacctaga atttcttcta cagcgtagcg
    12181 aatcaaatag ctccagcagg ccgtttgtgc gccaacgtag cagaattgcg ctaatactgc
    12241 ccagcgccag tggcgaatac gcgctaggcg agaaagcgat gcggagaacg atccttgttt
    12301 ggcgtcactg tgattatcac tctgcaatgc cgggaatttc gtcagcatga tcagcagggc
    12361 gaccagtaac acgatagcca cgatgatcat ataaggtgtc tgtaccgata ataccaggct
    12421 gtgtttatac gcactcaatt gctctggaga cattttatcg agaacgtctt gcgattgatg
    12481 tggcacgtta gacaaaataa gactttgccc aaagacaacc gcgataattg cgccaaacga
    12541 gttaaatgtt tgcgcaagat ttaagcggaa gtgaccactg ctttccggcc ctaataccgt
    12601 aacaaaaggg tttgcggcag tttccagaca acctaatccg gctgcaataa taaataggcc
    12661 aactaaaaat aaggtgtagt tcattatttc tgcagcgggc cagaataatg cagcgcccag
    12721 ggcatataaa aataacccag taataattcc tgctttataa ctgagttttt tcatcaatat
    12781 cccagcagga attgggataa tgaaataacc aaagtaaaag gccgattgga tcaggccagc
    12841 ctggaaattt gtcagcgtaa aagcctgctg gaattgaggt aataaaatgt cgttaaggtt
    12901 attggctacc gcccaaagaa aaaacagtga gcacagcagc gcgaatggaa taatgtaact
    12961 tctgctttgc cctgcatctt tatctaccgc acggtaactc tgcgtttgta ttgatgtgtt
    13021 tcccatagca tcctcttagg ttcagaagct taagcacgta atcggttatg ttttcaaaag
    13081 tgcaaaacca gcgagttcgg gcttcatatc agtgaatatt tgcctgtctg aaatgaactc
    13141 atggattcat tttcaaataa aaaacgctct gtaatatgac ggcggtcaca cttattagct
    13201 ggaaggtgac tttatgtgac taccatcact ttaataatcg aaatttcatg cccgtttcag
    13261 aaaataaatc ataaaaaaca caaaccagat gactgtatta attgtttatg ccgaaaacgg
    13321 gcattcctgg cttgggtgaa tagaaaaatg accgttttga taaatatttt taagagcggt
    13381 catttttaat ggggttataa aaagagaact tgcagttatt tactgtgatt agttgagcta
    13441 ggtcacaaaa tataagcttg tgaatttctt tcttgttaat caggaagaag atggtgggta
    13501 attaaacggc taattcaata gtgtgaaagg aacaacatta ttgccctgtt ttgaatcaga
    13561 gagaggtagc taatggaacg aaataaactt gctcgtcaga ttattgacac ttgcctggaa
    13621 atgacccgcc tgggactgaa ccaggggaca gcggggaacg tcagtgtacg ttatcaggat
    13681 gggatgctga ttacgcctac aggcattcca tatgaaaaac tgacggagtc gcatattgtc
    13741 tttattgatg gcaacggtaa acatgaggaa ggaaagctcc cctcaagcga atggcgtttc
    13801 catatggcgg cctatcaaag cagaccggat gccaacgcgg ttgttcacaa tcatgccgtt
    13861 cattgcacag cagtttccat tcttaaccga ccgatccccg ctattcacta catgattgcg
    13921 gcggcaggtg gtaattctat tccttgcgcg ccttatgcga cctttggaac acgcgaactt
    13981 tctgaacatg ttgcgctggc tctcaaaaat cgtaaggcaa ctttgttaca acatcatggg
    14041 cttatcgctt gtgaggtgaa tctggaaaaa gcgttatggc tggcgcatga agttgaagtg
    14101 ctggcgcaac tttacgtgac gaccctggcg attacggacc cggtgccagt gctgagcgat
    14161 gaagagattg ccgtagtgct ggagaaattc aaaacctatg ggttacggat tgaagagtaa
    14221 tttcgtaaag caacaaggag aaggatgatg gctaacagaa tgattctgaa cgaaacggca
    14281 tggtttggtc ggggtgctgt tggggcttta accgatgagg tgaaacgccg tggttatcag
    14341 aaggcgctga tcgtcaccga taaaacgctg gtgcaatgcg gcgtggtggc gaaagtgacc
    14401 gataagatgg atgctgcagg gctggcatgg gcgatttacg acggcgtagt gcccaaccca
    14461 acaattactg tcgtcaaaga agggctcggt gtattccaga atagcggcgc ggattacctg
    14521 atcgctattg gtggtggttc tccacaggat acttgtaaag cgattggcat tatcagcaac
    14581 aacccggagt ttgccgatgt gcgtagcctg gaagggcttt ccccgaccaa taaacccagt
    14641 gtaccgattc tggcaattcc taccacagca ggtactgcgg cagaagtgac cattaactac
    14701 gtgatcactg acgaagagaa acggcgcaag tttgtttgcg ttgatccgca tgatatcccg
    14761 caggtggcgt ttattgacgc tgacatgatg gatggtatgc ctccagcgct gaaagctgcg
    14821 acgggtgtcg atgcgctcac tcatgctatt gaggggtata ttacccgtgg cgcgtgggcg
    14881 ctaaccgatg cactgcacat taaagcgatt gaaatcattg ctggggcgct gcgaggatcg
    14941 gttgctggtg ataaggatgc cggagaagaa atggcgctcg ggcagtatgt tgcgggtatg
    15001 ggcttctcga atgttgggtt agggttggtg catggtatgg cgcatccact gggcgcgttt
    15061 tacaacactc cacacggtgt tgcgaacgcc atcctgttac cgcatgtcat gcgctataac
    15121 gctgacttta ccggtgagaa gtaccgcgat atcgcgcgcg ttatgggcgt gaaagtggaa
    15181 ggtatgagcc tggaagaggc gcgtaatgcc gctgttgaag cggtgtttgc tctcaaccgt
    15241 gatgtcggta ttccgccaca tttgcgtgat gttggtgtac gcaaggaaga cattccggca
    15301 ctggcgcagg cggcactgga tgatgtttgt accggtggca acccgcgtga agcaacgctt
    15361 gaggatattg tagagcttta ccataccgcc tggtaaatgc gctgatgtga taatgccgga
    15421 tacgacgcta gccgcgtctt atccggtata taagtgcatt aaattcaatg tgttgataat
    15481 cgtttgcatc cggctcgccg ttaccgtacc aaccgcaatt gctgtaaatt gccgtcgata
    15541 tgcaaatcgg tttgtaagcg ggcaatatcg cggcacagaa acgccatctc tttatgggtt
    15601 tctaattttt tgcgccactt ttcggcaacc gcatccagat tctcatatat cccttccaga
    15661 ctctgaaact cgaccagcag ctgcgtggcg ctttttggtc cgattcccgc aacacccggt
    15721 acctttgaac tgctgatccc cgcaagtccc cagtaatcgg gcaactgctg cggttgaacg
    15781 ccaaattctt tatcgataaa tggcgcatcc agccaacgtt tctggaagta atcacgaata
    15841 cgtaatgtcg gtgaaagtaa ctgacagtag cctttatctg tcgaaacaat cgttgcctga
    15901 tgcccggcct gtgtcacttt gaccgccagc gtggcggcta agtcatcggc ttcgttgccg
    15961 ctggttgacc agcacgggac gccgcgttgc tcaaaggcgg cgcgtaatgc aggcatctcg
    16021 tcgtgcaact cttccggcat tggcggtcga cccgctttgt aatctggtaa acgctgatga
    16081 cgccagccgc tactgcggtt ttcatcatca aaaacggcga ccgcgtgggt tggctggctg
    16141 tgcataatga gctgatcgag cgcatgctgg caggtttcga cacagggcga tccctgaacg
    16201 gcatgaatgc gacgaataag attcagtgca tcgacaataa gcaaatgaac agccacgata
    16261 acccccttaa agatcaggcg ataagggtaa cattcgatgc cggaacaggc tacggctgga
    16321 tgagaaatcg ggaagaggcc tcgcaaaacg aggcctctgg agagcgatta atcgcaggca
    16381 acgatcttca ttgccaggcc gccgcgagag gtttcgcggt acttggcgtt catatcttta
    16441 cctgtttcgt acatggtttc gataacttta tcgaggcaga cgcgcggctc gctggtacgg
    16501 cgtagcgcca tacgtgcggc gttcaccgct tttactgccg caatggcgtt acgctcgatg
    16561 catggcacct ggacctgtcc ggcgaccggg tcacacgtca gaccgaggtt gtgctccatg
    16621 gcgatttccg ccgcgatgca cacctgcgcc gggcttgcac ctaacagttc tgccagacca
    16681 gccgccgcca ttgagcacgc cacaccaact tcaccctggc agccgacttc agcaccagaa
    16741 attgacgcgt tcatcttata aagagaacca atggcactgg ctaccagcag gtaacgagcc
    16801 agtgagttag cgttcacttc gcggatgaac ttgtcgtagt acgccagcac tgccgggata
    16861 atcccgcacg caccgttagt cggcgcagtc accacgcgtc cgccagcagc gttctcttcg
    16921 ttcactgcca gtgcaaacat gttgatccag tcaacaaccg ccatcgggtc agtggtggtt
    16981 ttatcctggc tgaccagcat ccggcgtagt gccgcagcac ggcgtggaac gcgcagtttg
    17041 ccaggcaaca cgccttcggt ggaaataccg cgctcaatac cgccacgcat gacttcccat
    17101 acgttcgcca ggtgctgttc cagctcttct ttgctgtgca gcgccagctc atttttcatc
    17161 atcaggccag agagtgacag cccggtttcc tgacaatgtt tttgcagatc ggctgctgaa
    17221 ctgtacggat aaggaacttc aaccggtgcg ctattctgct ggccaaaatg ctcttcatca
    17281 acgataaagc caccgccaat agagtagtaa gtctggctgt aaacgacttt atcgcccgcc
    17341 agcgcggtaa tgcgcatacc gttttcatgc agagaaaggt tgtctgcatg gaagttcatg
    17401 cactgatcaa ccgggaactc cacttcatgc tgaccgtttg ccagcatcag gcgaccatga
    17461 gtattcacat cctgaataaa accagggatg gaatcgatat ccacggtatc cggcaggtta
    17521 cccgccaggc ccataataat ggcgatatca gtgtggtggc ctttaccggt cagagagagc
    17581 gagccgtaca cgtcaaccac cacgcgggtc acgtctttaa gcagattacg ggcaatcaga
    17641 tcgtcggtaa attgtttacc cgctttcatt ggtccaacgg tatgagaact ggaagggcca
    17701 atgccgattt tgaaaatatc gaatacgcta atcataggaa atacatcgcg ttaaaacgga
    17761 ggaagcgccg cccgaaagcg gcgcgaaagg acttagctga acagagagta gaagattgcg
    17821 gagattgcaa tcagacccat cacgacaacg aatacgttgc tgatgtgacc gctgtactta
    17881 cgcattgccg gtactttctg aattgcgtac atcggcatca ggaacaggat catcgcgatg
    17941 attgggccgc ccagggtttc aatcataccc aggatgctcg ggttcagggt ggcaacaatc
    18001 caggtcgtta ccagcatgaa cagcgcagtg atacggttca gcttgttgat ttcgatagac
    18061 ttacctttac cacgcagaga tttaatcacc ataccgttga agccttcacg tgcgcccagg
    18121 tagtggccga ggaaggattt ggtgatagcg ataatcgcga taatcggagc catccacgcg
    18181 ataaccggtg cgttaaagtg gttagccagg taagacagaa tcgagatgtt ctgctcttta
    18241 gccgcagcca ggtctgccgg agtcaggctc agtacacagc tgaagacgaa gaacattacg
    18301 gtcagcacca tcatgatgtg tgcgaatgcc aggatcttcg agcatttctg ttctgccata
    18361 tcgccgtact cttcacgctt cgcaacggcg aaagaagaga tgatcggaga gtggttgaaa
    18421 gagaacacca taaccggaat tgccagccac agggtcatcc acagaccgtt tccggttgca
    18481 gatgcagtgt ccagagacag cgtttccagt gctgcgccgt tccactgcgg gatcaggtac
    18541 agagccagca gcatcagtac gccaacaaac gggaatacca gaatactcat tgctttaacg
    18601 atcatctgct caccgaagcg aacgatggtc atcataccca cgatcaggat cagcgacaga
    18661 atcgcacgcg gcggtggtgt catacccagc tggtgagaca tgaagctttc aactgtattg
    18721 gtgattgcca cgctataaac cagcaggatc gggtagatag cgaagaagta gagcagggta
    18781 atcagtttac ctgcgccaat accaaagtgt tcttctacaa cctcggtgat gtcttcgcct
    18841 gggtttttac cagacagtac gaagcgagtc aggccgcggt gagcaaaaaa tgtcatcggg
    18901 aacgcaagga tagccatgat gatcagcggg atcataccgc caacaccggc gttgattggc
    18961 aggaacagca cgcccgcgcc gattgccgtg ccgtaaaggc ccagcatcca catggtgtct
    19021 gtcttgcgcc aggcactacg agagtctttc gacgcaatcg tgctggtttg agtcgtttcc
    19081 atctatttct cctggaggaa tgcaaataat tcagctttta gccagttcag ctcgggaagg
    19141 tgcctgaccg taaaaatgaa atctgtctat cggcgatggc ttaatcattt accgccgtaa
    19201 taatttgagg gcggaaagat acacacaagt tatgtatcta tcagtgatct tgatcccaaa
    19261 tacaataatc tacttggaag atagattatc ccaacgcttt actctgtaaa tcaaaagtaa
    19321 aagagaattt aagggcaata tttgcccatt aaacgacatt tgacttcttc cggggcgcgc
    19381 aatatatgta ggttgaactg aaaaaatcaa tacttttagt taatcatggc gataatttct
    19441 ttttcatagc gataaaatgt ggtttatgaa aatagggaaa cgtttgcgtt acaacataaa
    19501 ttgtatgaca tattaactgc aatgatattt atcgcgtgag ggtggtaaga aacattcata
    19561 taacaaatga aataaattga tgggtatgtg accaaaaacg agaaaacccg gcaacaaacg
    19621 tcaccggggg agggagatta cgtgcagatt tcgtagcaag ggatgtaggc tgagcctggc
    19681 aacttcatac gatgctgggc aacaaaaccc tgtagcaggt cgtccatacg acgcataatc
    19741 tctttatcgc cgttgatttt gtaaggacca aactcttcaa tggcgcgaat accgacttct
    19801 tttacgttac ccgccacaat accggagaac gcacggcgca gatcggcagc cagcacttca
    19861 acaggttgat ccgggtaaag cttcagatta gccatattct cgtgagacgg ctcaaacggc
    19921 atttgcaaat ctggcgcaat gcgcattgac cagttaaagc tgtaggcatc gcctgtgtcg
    19981 cggcgatttt ctttcaccag cggcatcgat tttttcatct gacgagcgac ttcagcggcg
    20041 tcatcaatga tgatgcggta atggcggcgc gcgttttcac ccaacgtatg cacgacaaac
    20101 tcgtccagta cgcggaagta gtcggcgctc tctttcgggc cggtgaggaa caatggtaaa
    20161 acctgatctt tgttggccgg gttcattaaa attcccagca aatacagcaa ctcttctgcc
    20221 gtacccacac cgccagggaa gataatgatg ccgtgagcga tacggacaaa cgcttccaga
    20281 cgtttttcga tatctggcat gatgatcaat tcgttgacca gcgggttagg cggttcagcg
    20341 gcgataatcg acggctctgt cataccaata aaacgactgt ctttgtaacg ctgctgtgcg
    20401 tgtccgaccg cagcaccttt catcggcgct tccatcgctc ccggaccaca gccggtgcag
    20461 atattcagct cacgcaggcc cagctgattc ccgacgcgac gggcatacaa atactcgttt
    20521 tcgttaattg agtgtccgcc ccagcagacc accatatttg gcgcttcacc gacatgcagt
    20581 gcgcgagcgt tacgcaagat ggaaaagacc aggttagtga tgtggacgga gttgtcgaga
    20641 ttcaggttgg gaaaacgaac ggtgttatgg atttgcccgt aaacgaagag aatgtcacgc
    20701 agaaccgcga acaagttggc ctgcaacgcg cgaataattc gcccatcgac aaaagcctct
    20761 tccgggggat taatcagttc cagctttacg ccgcgttcac ggcgcaggac gttaatatcg
    20821 aaattttcaa aacgagacag caattctttg ctgttatcgg tcaaactacc ggagttcagt
    20881 acggcaagtg aacagttgcg aaacagttga tagaggtcgc tgctggcggt gcgtttaagc
    20941 atatccactt ccagctgcga caacatatcc atggagccaa gcgggctaat atgtgtaatc
    21001 aagaaaaact ccttatggga cgaatatcgt cattcccttt gattacaata gccctgcctg
    21061 atgtcctttc acaaccttct gcgtgaatcc agcacgcaaa attgagaaaa aatttattgc
    21121 cgaaccagtc ttgtggtcga tgggacaaaa tcgctattac tgcgccacgg gttaatgtcc
    21181 agaccgccac gacgggtata acgtgcgtaa acgctcaatt tttctggctg gcagaagcgt
    21241 aacaggtcat taaagatgcg ttccacgcac tgttcgtgga actcgttgtg atgacggaat
    21301 gagaccaggt aacgcagcag tttttctctg tcaatttggc gtccacgata ctgaatttgg
    21361 atcgaacccc aatctggttg atgggtgatc aggcagtttg atttcagcag gtggctgaca
    21421 agcgtctctt ccactacttt ttcaccacag gtggcattct ccagatagtc agtagtgaat
    21481 tcatagttat cgatagtgat atcctggtca tcaatgcaag tgccattaaa atgacctatc
    21541 ggctggcctt ccagttcatc aagacgatat aacgccacgc taatcttacc ctgagcgcaa
    21601 gtgcttaagt cgcgctccag cgtctggcgc acctcatccc agttattaaa acgcgtctgg
    21661 ttaaaactgt tgagatagag cttaaaactc ttcgactcaa tcagatttac gctggtgtaa
    21721 tcaagttcaa catgaccgac agcgacctgc ggcaaacctt tcgcattcag ccaggaaagt
    21781 tcatacagcg tccagatatc cgtaccgtga aaaggcaggt tatccgcttt cagacccagc
    21841 gggtcgcgat tcaggctgcg tggaacgcct tgcagtaggc tggcgtcata ggtatcccgg
    21901 taatcggttg attttccaag agtcaggccc gcaagtgcct gatggtttgc ataagaagac
    21961 atgtttcacc gtcataaacc agataaacta aacgacaagt gtatctggtt taagtgaaga
    22021 gagaaattag tggacgattt gaccgcacaa gccctgaaag attttactgc acgctactgc
    22081 gatgcgtggc atgaagagca taaaagctgg ccgttaagtg aggaactgta cggtgttcct
    22141 tcaccgtgca ttatttcaac taccgaagat gccgtatact ggcaacccca gccgtttacc
    22201 ggggaacaaa atgtaaacgc ggttgaacgc gcttttgata ttgtgataca acccacaatt
    22261 catacctttt atacgactca gtttgccggg gatatgcacg cgcagtttgg cgatatcaaa
    22321 ctgacattgc tgcaaacctg gagtgaagac gacttccgtc gggtgcagga aaatctgatt
    22381 ggtcatctgg tgactcagaa aaggctaaaa ctgccgccca cgctatttat cgcgacgctg
    22441 gaagaagagt tagaggtcat ctcggtgtgt aatctctccg gtgaagtgtg caaagagacg
    22501 ctgggaacac gtaaacggac gcatttagcc tcaaatcttg cggaattcct taatcaactt
    22561 aagcctcttc tgtaatccaa ataccccatc tggttgtgag agatctctta cagaccctgt
    22621 aggagatcgc caggaaataa gcgaatactt aaaaagataa gaatcgttat tttcatttaa
    22681 aatcaaaatg ttgattgtta attcttaact ttcatatgaa attttcctta aggcattgtc
    22741 tgtaagcgcc ttgtaagact tcgcgaaaaa gacgattcta tcttcgtcga cagggagtca
    22801 gacaacgaag tgaacatcag gatgatgaca cttctgcagg acacaccagg atggtgtttc
    22861 agggaaaggc ttctggatga agcgaagagg atgacgcagg acgcgttaaa ggacacctcc
    22921 aggatggaga atgagaaccg gtcaggatga ttcggtgggt caggaaggcc agggacactt
    22981 caggatgaag tatcacatcg gggtggtgtg agcaggaagc aatagttcag gatgaacgat
    23041 tggccgcaag gccagaggaa aagttgtcaa ggatgagcag ggagcaacaa aagtagctgg
    23101 aatgctgcga aacgaaccgg gagcactgtg aatacagtgc tccctttttt tattcctgct
    23161 atccttcgcg gcagtttttc ttattgaagt tgctttatga ccactcatga ccgcgttcgt
    23221 ttccagttgc agacgcttga agcgttactg cgtgaacatc agcactggcg aaacgatgaa
    23281 ccccagccgc atcaatttaa tagtacccaa ccgttcttta tggacaccat ggaaccactg
    23341 gagtggttgc agtgggtgct gatcccgcga atgcacgatt tgttaaataa caaccagccg
    23401 ttaccgggcg cttttgctgt tgcaccctat tatgaaatgg cgctggcgac ggaccatccg
    23461 caacgcgcgc ttattctggc tgagttggaa aaactggatg ctctttttgc ggatgatgcg
    23521 agctaatgct ggaaatactc tatcaggatg aatggctggt tgcggtaaat aaaccctccg
    23581 gctggctggt tcaccgtagc tggctggatc gcgacgagaa agtagtggtc atgcaaaccg
    23641 tgcgtgacca gataggacag catgttttta ctgctcatcg tctggaccga cccacttctg
    23701 gtgtgttgtt gatgggatta tccagcgagg ccggacggct gctggcacaa cagtttgaac
    23761 agcaccaaat ccagaaacgt taccatgcga ttgtgcgcgg ctggttgatg gaagaagcgg
    23821 tgctggatta tccactggtg gaagaactgg acaaaatcgc tgataaattt gcccgcgaag
    23881 ataaaggccc gcagccagca gtgacgcatt atcgcggtct ggcgaccgta gaaatgccgg
    23941 tagcgaccgg acgttacccg accacgcgct acggcctggt ggaactggag ccgaaaaccg
    24001 gacgcaaaca tcagcttcgc cgacatcttg cccatttgcg tcatccgatt attggcgata
    24061 gcaaacatgg cgatttacgc cagaatcgca gcggtgctga gcattttggc ctccagcggt
    24121 taatgctaca tgccagtcag ttgtcattga cgcatccttt tactggcgag ccgctgacta
    24181 ttcacgcggg cctggacgac acctggatgc aggcgttatc acaatttggc tggcgcggtc
    24241 tgcttcctga aaatgaaagg gttgagttca gtgcgccatc gggtcaggat ggagagataa
    24301 gttcctaatc cagggagaaa acggtatggc ggaaattggt atttttgtcg gcaccatgta
    24361 cggaaattcg ctgttagtgg cggaagaagc agaagcaatt ctgaccgcac agggccacaa
    24421 agcaacggta tttgaagatc ctgaattaag cgactggctg ccttatcagg ataagtatgt
    24481 tctggtggtt acgtccacga ccgggcaggg cgaccttcct gatagcattg tgccgctctt
    24541 tcagggaatc aaagatagtc tgggcttcca gccgaatctg cgttatggcg tgattgcgct
    24601 cggcgacagc agttatgtaa atttctgcaa tggcggcaaa cagttcgatg ccttgttgca
    24661 ggaacagagc gctcagcggg ttggcgaaat gctgttgatt gacgccagtg agaacccgga
    24721 accggaaacg gaatcaaatc cgtgggtcga acagtggggc acgctgttgt cctgatatgt
    24781 tcagcgagcg gtaaatgtcg ttttagcggt gctgaatcga atcttttcag gcaaatgcca
    24841 gtaaaaactg cttcatagcg cggattttta ctggcgtttg cctggagtca agcgatccat
    24901 ttcatactct tctttatttc ttcgttttaa cccttccttt cttgttcttg ttttcatttc
    24961 cgtgaagtgg attccaccgt ccagggctaa tgccaaaatc gggcctcatt gaacgcatta
    25021 atgttgtgtt gttgcacggt gagccgctat ggcgcgcttt ttatactgct attgccagat
    25081 ataaacacgc gccgtattcg gcgaacgacc tataaaaacg gcaaaaaaca ccctacgtca
    25141 cctctgattt cctggctatg tcgcagtcca gagtgagcgt ggctaacgcg aattttcagg
    25201 agtgcaacaa tgagttcttt aagtcaggct gcgagcagtg tggaaaaacg cacgaatgct
    25261 cgttactgga tagtggtgat gttgtttatc gtcacatcct tcaactacgg cgaccgcgct
    25321 acgctttcta ttgccggttc ggaaatggcc aaagatatcg gccttgatcc cgtgggaatg
    25381 ggctatgtgt tctctgcctt ctcatgggct tatgttatcg ggcagatccc tggtggttgg
    25441 ttgctggatc gttttggttc aaaacgcgtc tacttctggt cgatctttat ctggtcgatg
    25501 tttaccttgc tgcaaggctt cgtcgatatc tttagtggat tcggcattat cgttgccctg
    25561 tttactctgc gcttcctggt cgggcttgct gaagcgccat ctttccccgg caacagtcgc
    25621 attgttgcgg cctggtttcc ggcgcaggaa aggggaacgg cggtgtcgat ttttaactcc
    25681 gctcaatact tcgcaacggt gatcttcgcg ccgattatgg gctggctgac gcatgaagtg
    25741 ggctggtcac acgtcttctt ctttatgggc ggtctgggga ttgtcatcag ctttatctgg
    25801 ttgaaagtca tccacgagcc aaatcaacat ccgggggtaa atcagaaaga gctggagtac
    25861 atcgccgcgg gtggcgcgct gatcaatatg gatcagcaaa acaccaaagt taaagtgccg
    25921 ttcagcgtga agtgggggca gatcaaacaa ctgctcgggt cacggatgat gatcggcgtt
    25981 tatatcggtc agtactgtat caacgccctg acttactttt tcattacctg gtttccggtt
    26041 tatctggtgc aggcgcgtgg gatgtcgatt ctgaaagcgg gctttgtggc ttccgttccg
    26101 gcggtttgcg gttttatcgg cggtgtgctg ggtgggatta tttccgactg gctgatgcgt
    26161 cgcacgggat cgctgaacat tgcgcgtaaa acaccgatcg taatgggcat gttgctgtcg
    26221 atggtgatgg tgttctgcaa ctacgtcaac gttgagtgga tgatcatcgg ctttatggcg
    26281 ctggccttct tcggtaaggg catcggggcg ctgggttggg cagtaatggc agataccgcg
    26341 ccaaaagaga tcagcggtct ttccggtggc ctgttcaaca tgttcggtaa catttctggc
    26401 atcgtcacgc caatcgcaat tggttatatc gttggcacga ctggctcctt taatggggcg
    26461 ctgatttatg ttggtgttca tgccttaatc gcggtactga gctacctggt gctggtgggc
    26521 gatatcaagc gtatcgagct gaaacctgtc gcggggcaat aagatgacga cacaatccag
    26581 tcctgttatt actgatatga aagtcattcc ggtcgccggg cacgatagca tgttgcttaa
    26641 tattggtggg gcacataacg catatttcac ccgcaatatt gtggtactca ccgataacgc
    26701 cgggcatacc ggcattggtg aagcgccggg cggagaggtg atttatcaaa cactggtcga
    26761 tgctattccg atggttctgg gccaggaagt tgcgcgcctg aataaagtgg ttcagcaggt
    26821 gcataaaggt aatcaggcag ccgattttga taccttcggc aaaggtgcct ggacttttga
    26881 attgcgcgtt aatgccgtgg cggcgctgga agccgccttg cttgacctgc taggtaaggc
    26941 gctgaatgtt ccggtctgcg aactgttggg gccaggcaag caacgcgaga ctattaccgt
    27001 cctcggttat ctgttttata tcggtgatcg gaccaaaacc gatcttcctt atctggaaaa
    27061 tacgccgggc aaccatgagt ggtatcagtt gcgccatcag aaagcgatga acagcgaagc
    27121 cgttgtgcgt ctggcggaag cctcacagga tcgctatggc tttaaagatt tcaaacttaa
    27181 gggcggggtg ttacctggcg agcaagaaat cgacactgtt cgtgcattga agaaacgctt
    27241 ccctgatgcg cggattaccg ttgatcccaa cggtgcctgg ctacttgatg aagccatttc
    27301 tctatgcaaa gggctgaatg atgttcttac ctatgccgaa gatccatgcg gcgcagaaca
    27361 gggtttctcc ggtcgtgaag ttatggcgga gtttcggcgg gcgaccggct tgcccgtcgc
    27421 gactaacatg atcgccacca actggcgcga aatgggtcat gcggtgatgc tcaatgcggt
    27481 agatattcca cttgccgatc cgcacttctg gacgctttcc ggtgcagtcc gtgtggcgca
    27541 gctttgcgac gactgggggc tgacctgggg ctgccattct aataaccatt tcgatatctc
    27601 tctggcgatg tttacccatg tgggcgcggc ggcaccgggt aatcctaccg ctatcgatac
    27661 ccactggatt tggcaggagg gcgattgtcg cctgaccaaa aatccgctgg agattaaaaa
    27721 cggaaaaatt gccgttcctg atgcgcccgg tctgggcgtg gaactggact gggaacaggt
    27781 acaaaaggca catgaggcct ataaacgtct gcctggcggt gcgcgtaacg acgcaggtcc
    27841 gatgcagtac ctgatccccg gctggacctt tgaccgtaaa cgtcccgttt tcggccgtca
    27901 ttgattctga aaaaggacat aaatatgagt tctcaattta cgacgtctgt tgttactgaa
    27961 atgcaggtca tcccggtggc gggtcatgac agtatgctga tgaatctgag tggggcacac
    28021 gcaccgttct ttacgcgtaa tattgtgatt atcaaagata attccggtca cactggcgta
    28081 ggggaaattc ccggcggcga gaaaatccgt aaaacgctgg aagatgcgat tccgctggtg
    28141 gtaggtaaaa cgctgggtga atacaaaaac gttctgacgc tggtgcgtaa tacttttgcc
    28201 gatcgtgatg ctggtgggcg cggtttgcag acatttgatt tgcgtaccac tattcatgta
    28261 gttaccggga tagaagcggc aatgctggat ctgctggggc agcatctggg ggtaaacgtg
    28321 gcatcgctgc tgggcgatgg tcaacagcgt agcgaagtcg aaatgctcgg ttatctgttc
    28381 ttcgtcggta atcgcaaagc cacacctctg ccgtatcaaa gccagccgga tgactcatgc
    28441 gactggtatc gcctgcgtca tgaagaagcg atgacgccgg atgcggtggt gcgcctagcg
    28501 gaagcggcgt atgaaaaata tggcttcaac gatttcaaac tgaaaggcgg tgtactggcc
    28561 ggggaagaag aagccgagtc tattgtggca ctggcgcaac gcttcccgca ggcgcgtatt
    28621 acgctcgatc ctaacggtgc ctggtcgctg aacgaagcaa ttaaaattgg taaatacctg
    28681 aaaggttcgc tggcttatgc agaagatccg tatggtgcgg agcaaggttt ctccgggcgt
    28741 gaagtgatgg cagagttccg tcgcgcgact ggcctgccga ctgcaaccaa tatgatcgcc
    28801 accgactggc ggcagatggg ccatacgctc tcgttacaat ccgttgatat cccgctggcg
    28861 gatccgcatt tctggactat gcaaggttcg gtacgtgtgg cgcaaatgtg ccatgaattt
    28921 ggcctgacct ggggttcaca ctcaaacaac cacttcgata tttccctggc gatgtttacc
    28981 catgttgccg ccgctgcacc gggtaaaatc accgcgattg atacgcactg gatttggcag
    29041 gaaggcaatc agcgcttgac caaagaaccg tttgagatca aaggcggtct ggttcaggtg
    29101 ccggaaaaac cggggctggg tgtagaaatc gatatggatc aagtgatgaa agcccatgag
    29161 ctgtatcaga aacacgggct tggcgcgcgt gacgatgcga tgggaatgca gtatctgatt
    29221 cctggctgga cgttcgataa caagcgcccg tgcatggtgc gttaagacag aaaaccaaaa
    29281 ccggctctat ctggagccgg cttgagactg atgctaaacg caaaatagcc tgttgtgcta
    29341 tgtatcaggt ctacatcatt ttttaattca ttgaatttaa ttggttttgt aggccggata
    29401 aggcgttaac gccgcatccg gcataaacac aggcactttg tcaccaatct gaaaccagcg
    29461 tcataaaaag ccgattgcta ctcgacaaga cgtccgttac ccgagaattt tgctggcttc
    29521 gcgcgcgaca ttatccatct cgtccaacag ttccagcagc tccggctcca aatcttcttc
    29581 tttagtaccg ctacgtagct gttgttcgat cagttggcag agattcttca tacgcggcac
    29641 accgctatag ccgcaactgc catgcagttt atgaatcaaa tcaaccaggc cttccgggtt
    29701 ttctccaacc agctgctcct caactttgtt gcgaacttca ggcaggaaat cgagtaacat
    29761 ttgcagcata tcgcgcgcta gatcggtttt tcctgctgcc tggcgtagtg ccagttgcca
    29821 gtcgagggtc gcattcgggt tcaccacaat ttcgttgact tcgggcgtca cgacgcgaga
    29881 ggaaataccg ctgccaggct tgtagcgcaa caacaaatta tgcaatcgct cttcttcaat
    29941 cggtttcgcc agataatcgc tcatccctgc gccaagcagc ttctcttttt gcccggccat
    30001 tgcatgcgcc gttaccgcga taaccggcgt ttgttgctga tgggggagct ggtggatgag
    30061 ctcgcaggcc cgaatgccat ccatgtcagg catttgaata tccattaaga tcaaatcgaa
    30121 cggcatctgt ttcgcccgtt caaccgcctg atgcccgcta tcgcaaagtt ccacatgttg
    30181 caccatatct tccagcaatg cgccgataag tttcaggtta gcggggttgt catcaactgc
    30241 catgactgtc attgccagct tgctttcatc ggttacaggc aaaagcgtgt tttgtttgtg
    30301 atgacaaaat tccgtcaggg caggcaacag gcgcgtaggt gttaatggtt tcagcagaca
    30361 cgcgccgata ccatcttgct tgagtttttc tgcattgact tgtgcatggc aaggaagtgc
    30421 cagcatcagg aaatcggtca tcgataccgc tttcgctaat cgctcatgtt gcattgttag
    30481 cggctcgcgg aaggtcaccg cgatgcctaa caacatcatg tcgtaatgcg cgggaggcag
    30541 cgcggagaac gttgggctat aaaccacttc cagcggcgtt tcacttaaaa tatccagcgt
    30601 gcattgcgct gctgcggagt ttggttcgac ataggccagg cgcttaccgg cgaggcactg
    30661 ggtggatggc ccttcgataa taatgttcgg gttcagatcg agattaatgt ggaaccagaa
    30721 agttgaaccg cgattcggct ggctatggaa cgaaatatcg ccgcccattt cattaaccag
    30781 tttttgtgta atcaccagcc ccagaccggt gccaccatga cggcgggaaa tactggcatc
    30841 agcctgtcgg aaggcctgga ataagcgcga ttgatcgcgt tcaggaatac caatgccggt
    30901 atcccgaatc tgcacttcaa tctgcacttt ggtattactc agcgcacgtt tttctaccag
    30961 aatatcaatg ttgccattct cggtgaattt aattgcattc cccaccaggt tagtgatgat
    31021 ttgctgtaat cgcagtgggt cgccgatcac gttatcaggc acgtcgcttt taatattgag
    31081 cgtcagttcc aaccctttat cgtgagaaga atgtgccagc agagtaacga cttcatccag
    31141 cgtgctgcgt agtgggaatg gaatactttc cagaatcagc ttacctgctt ccagtttcga
    31201 gaagtcgaga acatcattaa taattgccag caaattattt gccgaacgtt caatcgtatt
    31261 caggtgatcg cgctgcgttg gtgttaattc tgttttcagc gtcaggcggg taaagccaat
    31321 aacaccattc agtggtgtac gcagctcgtg tgacatattt gccagaaact cggatttaat
    31381 acgcgccgct tcctgggcgc gctttttcgc cagatctaac tcaacgttct gaatttccat
    31441 ctgctccagc gtttcacgca gatcggacgt cgcctggtcg atattgtgct gcatctcttc
    31501 gtgataagca gccagcgaca ttgccatcga gttgataccg tttttcagca tatccagctc
    31561 gccgagcata aatccttcca cccggctgtc gagttgcccg cgacggatgc ggtcgacggt
    31621 attcaccatg ttgcgaatcg gaccggttac atcgcgcatt aagcgccagc caaaaataag
    31681 cgcaataccg atacaaaaca gcatcatcac gctggaaata aagatctctt tatattgctg
    31741 caagcgaacc gatttaagat ccagctccag cgcaatatat cccaacatat tttgactatt
    31801 tttggcatcg ctactgggcg attcgtcggg ggagtaactc tcagaaataa tcggcgtgcg
    31861 gaggatcata atatcgccgt cacgagtgac agtgagctgg cgaggaaacg gcacgttgct
    31921 gccgagctgc attgatgagg gatcaagatg aaaattggag gtgacaaaga gtcggttatt
    31981 ttcatcataa accgaaatcg cgcgaacaat atcggaatgg cgacgatgca gtacgcttat
    32041 taactgaccg atagattcgc gattttgcag gctcatgcca tattcagtag aaactgcaag
    32101 cggctcaata atgctggctc cggcatcttc cagttgacgc tgcaagtcgt tatagcgatg
    32161 cacaacgaaa aagatactca gcaataaacc aataaggacg gtcggtgcca ggatcagaat
    32221 catcatgcgt gcgcgcaggc tgtagttggt catggagttc cgttatggga caattaaggt
    32281 cacactgtta aattgagaaa gtctcgccaa tggcgcaatt ctactctgca aaacgacgca
    32341 cgacgacgcg tcagatcata accgtttcag tcaacgacct cgactctttt ggtcagggcg
    32401 tggcgcgaca taacggcaaa acgctattta tccccggatt attgccgcag gaaaacgcgg
    32461 aagttactgt tactgaagat aaaaaacagt atgcccgcgc taaagtcgta cgccggttaa
    32521 gcgatagccc gggacgcgaa acgccacgct gtcctcattt tggcgtatgc ggcggctgtc
    32581 agcaacaaca cgccagcgtg gatttacagc agcgaagcaa aagtgcggca ctcgcccgat
    32641 taatgaaaca cgaagtctct gaagtgatcg ccgatgttcc ctggggctat cgccgtcgcg
    32701 cgcgtttaag tctgaactac ttaccgaaaa cacagcaact tcagatgggg tttcgcaaag
    32761 cgggctccag tgacattgtc gacgttaaac aatgccccat tttagtgccc caacttgaag
    32821 cattgctgcc caaagtcatg gcatgcctgg gcagcttaca agttatgcgc catcttggtc
    32881 atgttgaact ggtacaggca accagcggca cgctgatgat tttgcgccat accgcaccgc
    32941 taagttcggc agatcgcgaa aaactggaac gcttttcgca ttctgaaggc ctggatctgt
    33001 atctcgcccc cgatagtgag atactcgaaa ccgtctctgg tgagatgccc tggtatgact
    33061 caaatgggtt gcgcttaact tttagcccgc gcgattttat tcaggtcaat gcgggtgtga
    33121 accaaaaaat ggtagcgcgt gcgttggaat ggctggatgt gcaacctgaa gatcgcgtac
    33181 tggatttgtt ctgcggtatg ggcaacttta cactgccatt ggcgacacaa gctgccagtg
    33241 tggtgggtgt agaaggcgtt ccggcgctgg tggaaaaagg ccagcagaat gcgcgtctta
    33301 atggcttaca gaatgtgacg ttttatcacg aaaatcttga agaagatgtc acaaagcagc
    33361 cgtgggcgaa aaacggcttc gataaagtgt tgctggaccc ggcgcgagca ggtgccgcag
    33421 gtgttatgca gcaaattata aaactggaac ctattcgtat agtttatgta tcctgtaacc
    33481 ctgcaacgct ggctcgggat agcgaagcgt tattaaaagc aggatatacc attgcgcgac
    33541 tggcgatgct ggatatgttc ccacacacgg gacatctgga atcgatggta cttttctcgc
    33601 gcgttaaata gttgcgattt gccgatttcg gcaggtctgg tccctaaagg agaggacgat
    33661 ggttgcggta agaagtgcac atatcaataa ggctggtgaa tttgatccgg aaaaatggat
    33721 cgcaagtctg ggtattacca gccagaagtc gtgtgagtgc ttagccgaaa cttgggcgta
    33781 ttgtctgcaa cagacgcagg ggcatccgga tgccagtctg ttattgtggc gtggtgttga
    33841 gatggtggag atcctctcga cattaagtat ggacattgac acgctgcggg cggcgctgct
    33901 gttccctctg gctgatgcca acgtagtcag cgaagatgtg ctgcgtgaga gcgtcggtaa
    33961 gtcggttgtt aaccttattc acggcgtgcg tgatatggcg gcgatccgcc agctgaaagc
    34021 gacgcacact gattctgttt cctccgaaca ggtcgataac gttcgccgga tgttattggc
    34081 gatggtcgat gattttcgct gcgtagtcat caaactggcg gagcgtattg ctcatctgcg
    34141 cgaagtaaaa gatgcgccgg aagatgaacg tgtactggcg gcaaaagagt gtaccaacat
    34201 ctacgcaccg ctggctaacc gtctcggaat cggacaactg aaatgggaac tggaagatta
    34261 ctgcttccgt tacctccatc caaccgaata caaacgaatt gccaaactgc tgcatgaacg
    34321 gcgtctcgac cgcgaacact acatcgaaga gttcgttggt catctgcgcg ctgagatgaa
    34381 agctgaaggc gttaaagcgg aagtgtatgg tcgtccgaaa cacatctaca gcatctggcg
    34441 taaaatgcag aaaaagaacc tcgcctttga tgagctgttt gatgtgcgtg cggtacgtat
    34501 tgtcgccgag cgtttacagg attgctatgc cgcactgggg atagtgcaca ctcactatcg
    34561 ccacctgccg gatgagtttg acgattatgt cgctaacccg aaaccaaacg gttatcagtc
    34621 tattcatacc gtggttctgg ggccaggtgg caaaaccgtt gagatccaaa tccgcaccaa
    34681 acagatgcat gaagatgcag agttgggtgt tgctgcgcac tggaaatata aagagggcgc
    34741 ggcagctggc ggcgcacgtt cgggacatga agaccggatt gcctggctgc gtaaactgat
    34801 tgcgtggcag gaagagatgg ctgattccgg cgaaatgctc gacgaagtac gcagccaggt
    34861 ctttgacgac cgggtgtatg tctttacgcc gaaaggtgat gtcgttgatt tgcctgcggg
    34921 atcaacgccg ctggacttcg cttaccacat ccacagtgat gtcggacacc gctgcatcgg
    34981 ggcaaaaatt ggcgggcgca ttgtgccgtt cacctaccag ctgcagatgg gcgaccagat
    35041 tgaaattatc acccagaaac agccgaaccc cagccgtgac tggttaaacc caaacctcgg
    35101 ttacgtcaca accagccgtg ggcgttcgaa aattcacgct tggttccgta aacaggaccg
    35161 tgacaaaaac attctggctg ggcggcaaat ccttgacgac gagctggaac atctggggat
    35221 cagcctgaaa gaagcagaaa aacatctgct gccgcgttac aactttaatg atgtcgacga
    35281 gttgctggcg gcgattggtg gcggggatat ccgtctcaat cagatggtga acttcctgca
    35341 atcgcaattt aataagccga gtgccgaaga gcaggacgcc gccgcgctga agcaacttca
    35401 gcaaaaaagc tacacgccgc aaaaccgcag taaagataac ggtcgcgtgg tagtcgaagg
    35461 tgttggcaac ctgatgcacc acatcgcgcg ctgctgccag ccgattcctg gagatgagat
    35521 tgtcggcttc attacccagg ggcgcggtat ttcagtacac cgcgccgatt gcgaacaact
    35581 ggcggaactg cgctctcatg cgccagaacg cattgttgac gcggtatggg gtgagagcta
    35641 ctccgccgga tattcgctgg tggtccgcgt ggtggctaat gatcgtagtg ggttgttacg
    35701 tgatatcacg accattctcg ccaacgagaa ggtgaacgtg cttggcgttg ccagccgtag
    35761 cgacacaaaa cagcaactgg caaccatcga catgaccatt gagatttaca acctgcaggt
    35821 gctgggccgc gtgctgggta aactcaacca ggtgccggat gttatcgacg cgcgtcggtt
    35881 gcacgggagt taggcagaaa tttgctcgta tctacaatgt agattgatat atactgtatc
    35941 tacatatgat agcggtttga ggaaagggtt atgatccaca gtagcgtaaa gcgttgggga
    36001 aattcaccgg cggtgcggat cccggctacg ttaatgcagg cgctcaatct gaatattgat
    36061 gatgaagtga agattgacct ggtggatggc aaattaatta ttgagccagt gcgtaaagag
    36121 cccgtattta cgcttgctga actggtcaac gacatcacgc cggaaaacct ccacgagaat
    36181 atcgactggg gagagccgaa agataaggaa gtctggtaat ggtaagccga tacgtacccg
    36241 atatgggcga tctgatttgg gttgattttg acccgacaaa aggtagcgag caagctggac
    36301 atcgtccagc tgttgtcctg agtcctttca tgtacaacaa caaaacaggt atgtgtctgt
    36361 gtgttccttg tacaacgcaa tcaaaaggat atccgttcga agttgtttta tccggtcagg
    36421 aacgtgatgg cgtagcgtta gctgatcagg taaaaagtat cgcctggcgg gcaagaggag
    36481 caacgaagaa aggaacagtt gccccagagg aattacaact cattaaagcc aaaattaacg
    36541 tactgattgg gtagtgttac taactcactt ctattctggt cacaggtttc cacctgacag
    36601 acccgcaatt ttcaggacaa ttcaatgaat caaatcgacc gtttgctcac tattatgcag
    36661 cgcctgcgcg atccggaaaa cggctgcccg tgggataaag agcagacatt tgccaccatt
    36721 gcgccttaca cccttgaaga aacctacgaa gtgctggacg ccatcgcccg tgaagatttt
    36781 gacgatcttc gcggtgaact gggcgatctg ctattccagg tggtgtttta cgcgcaaatg
    36841 gctcaggaag aagggcgctt tgactttaat gatatttgcg ctgctattag cgataaatta
    36901 gagcgtcgcc atccgcatgt ttttgctgat agttctgccg aaaacagtag tgaagtgctt
    36961 gcccgttggg agcaaatcaa aaccgaagag cgcgcgcaga aagcgcagca ttcggcgctg
    37021 gacgatattc ctcgtagttt accggcttta atgcgggcgc aaaaaatcca gaaacgttgc
    37081 gccaacgttg gcttcgactg gacgacgctt ggcccggtgg ttgataaagt ctacgaagag
    37141 atcgacgagg tgatgtacga ggcgcggcag gctgttgtcg accaggctaa actggaggag
    37201 gaaatggggg acctgctgtt tgccacggtt aatctggctc gccatttagg gacgaaagca
    37261 gaaatcgcat tgcaaaaagc gaacgaaaaa ttcgagcgtc gttttcgcga agtggagcgt
    37321 attgttgccg cgcgtggact ggaaatgaca ggtgttgacc tcgaaacaat ggaagaagtc
    37381 tggcaacagg taaaacggca ggaaattgat ctctaaggga attacgcggt caagcgccat
    37441 ttgtgtcatt ttttaaatga caagcgcttg atttgcgtca aaaacattta ccccaaaggg
    37501 gctattttct cactcctgat ttcaatagtg cgctggcgaa gaggagggat aatgaaagtt
    37561 tgtggcacag gtcatgttcg ggtatactgc tttcccgtcc tggttattcc atcgtctttt
    37621 caacctaact tctcaggttc agcatgacaa cgaactatat ttttgtgacc ggcggggtcg
    37681 tatcctctct gggtaaaggc attgccgcag cctccctcgc agccattctt gaagcccgtg
    37741 gcctcaatgt gaccatcatg aaactggatc cgtacatcaa cgtcgatcca ggtactatga
    37801 gcccaatcca acacggggaa gtgttcgtta ctgaagacgg cgctgaaacc gacctggacc
    37861 tggggcacta cgagcgtttc attcgtacca aaatgagccg ccgcaacaac ttcaccacgg
    37921 gtcgtatcta ctctgacgtt ctgcgtaaag aacgccgcgg tgactacctc ggcgcaaccg
    37981 tgcaggttat tccgcacatc actaacgcaa tcaaagagcg cgtgctggaa ggtggcgaag
    38041 gtcatgacgt agtactggta gaaatcggcg gtacagtagg tgatatcgaa tccttgccgt
    38101 tcctcgaagc gattcgccag atggctgttg aaattggccg tgagcacact ctgtttatgc
    38161 acctgacgct ggtgccgtac atggcagcgt ctggtgaagt caaaaccaaa ccgactcagc
    38221 actctgtaaa agagctgctc tccatcggta tccagcctga catcctgatt tgtcgttcag
    38281 atcgcgctgt tccggcgaac gaacgtgcga agattgcatt gttctgtaat gttccggaaa
    38341 aagcggttat ttctctgaaa gacgtcgatt ccatctataa aattccgggc ctgttgaaat
    38401 ctcaggggct ggacgattat atttgtaaac gattcagctt aaactgcccg gaagcaaatc
    38461 tgtccgaatg ggaacaggtt atcttcgaag aagcgaatcc ggtaagtgaa gtcaccatcg
    38521 gtatggtcgg caagtacatt gaactgccgg atgcttataa atcagtgatc gaagcactga
    38581 aacacggtgg gctgaagaat cgtgtcagcg tcaacatcaa actgatcgat tcacaagatg
    38641 ttgaaacgcg cggcgttgaa atccttaaag gtctggacgc aatcctcgta cctggcggtt
    38701 tcggctatcg tggcgtagaa ggcatgatta cgaccgcgcg ttttgcgcgt gagaacaata
    38761 ttccttatct gggcatttgc ctgggtatgc aggtggcgtt aattgattac gctcgccatg
    38821 ttgccaacat ggagaacgcc aactctacgg aatttgtgcc agactgtaag tacccggttg
    38881 tggcgctgat taccgagtgg cgcgatgaaa acggcaacgt tgaagttcgt agcgagaaga
    38941 gcgatctcgg cggtaccatg cgtctcggcg cacagcagtg ccagttggtt gacgatagcc
    39001 tggttcgcca gctgtacaat gcgccgacaa ttgttgagcg tcatcgtcac cgttacgaag
    39061 tcaacaacat gctgttgaaa cagattgaag atgcaggtct gcgcgttgcg ggccgttccg
    39121 gggatgatca gttggtcgag atcatcgaag ttccgaatca cccgtggttc gtggcttgcc
    39181 agttccatcc ggagtttact tctactccac gtgatggtca cccgctgttt gcaggctttg
    39241 tgaaagctgc cagcgagttc cagaaacgtc aggcgaagta agtaaaaaag ttagaacggc
    39301 aacgcgtacc ttgggtacgc gttgtttgtc tggagtttca gtttaactag tgacttgagg
    39361 aaaacctaat gtccaaaatc gtaaaaatca tcggtcgtga aatcatcgac tcccgtggta
    39421 acccgactgt tgaagccgaa gtacatctgg agggtggttt cgtcggtatg gcagctgctc
    39481 cgtcaggtgc ttctactggt tcccgtgaag ctctggaact gcgcgatggc gacaaatccc
    39541 gtttcctggg taaaggcgta accaaagctg ttgctgcggt aaacggcccg atcgctcagg
    39601 cactgattgg caaagacgct aaagatcagg ctggcattga caagatcatg atcgacctgg
    39661 acggcaccga aaacaaatcc aaattcggcg cgaacgcaat cctggctgta tctctggcta
    39721 acgccaaagc tgctgctgcc gctaaaggga tgccgctgta cgagcacatc gctgaactga
    39781 acggcactcc gggcaaatac tccatgccgg ttccgatgat gaacatcatc aacggtggtg
    39841 agcacgctga caacaacgtt gatatccagg aattcatgat tcagccggtt ggcgcgaaaa
    39901 ctgtgaaaga agccatccgc atgggttctg aagttttcca tcacctggca aaagttctga
    39961 aagcgaaagg catgaacact gcagttggtg acgaaggtgg ctatgcgccg aacctgggtt
    40021 ccaacgctga agctctggct gttatcgctg aagctgttaa agctgctggt tatgaactgg
    40081 gcaaagacat cactctggcg atggactgcg cagcttctga attctacaaa gacggtaaat
    40141 acgttctggc tggcgaaggc aacaaagctt tcacctctga agaattcact cacttcctgg
    40201 aagaactgac caaacagtat ccgatcgtat ctatcgaaga cggtctggac gaatctgact
    40261 gggacggttt cgcataccag accaaagttc tgggcgacaa aatccagctg gttggtgacg
    40321 acctgttcgt aaccaacacc aagatcctga aagaaggtat cgaaaaaggt atcgctaact
    40381 ccatcctgat caaattcaac cagatcggtt ctctgaccga aactctggct gcaatcaaga
    40441 tggcgaaaga tgctggctac actgcagtta tctctcaccg ttctggcgaa actgaagacg
    40501 ctaccatcgc tgacctggct gttggtactg ctgcaggcca gatcaaaact ggttctatga
    40561 gccgttctga ccgtgttgct aaatacaacc agctgattcg tatcgaagaa gctctgggcg
    40621 aaaaagcacc gtacaacggt cgtaaagaga tcaaaggcca ggcataagac tgactttatc
    40681 tgatttaaaa atgccagcct cc
//
LOCUS       DAZOZY010000014        40113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZOZY010000014 DAZOZY010000000
VERSION     DAZOZY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.014"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHURQ_000661"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHURQ_000661"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR0822524.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHURQ_000662"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHURQ_000662"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR0822525.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHURQ_000663"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHURQ_000663"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR0822526.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHURQ_000664"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHURQ_000664"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR0822527.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHURQ_000665"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHURQ_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR0822528.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHURQ_000666"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHURQ_000666"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR0822529.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHURQ_000667"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHURQ_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR0822530.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHURQ_000668"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHURQ_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR0822531.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHURQ_000669"
     CDS             6537..6773
                     /locus_tag="ACHURQ_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR0822532.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHURQ_000670"
     CDS             6776..7633
                     /locus_tag="ACHURQ_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR0822533.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHURQ_000671"
     CDS             7654..9645
                     /locus_tag="ACHURQ_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR0822534.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHURQ_000672"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHURQ_000672"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR0822535.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHURQ_000673"
     CDS             complement(10248..11144)
                     /locus_tag="ACHURQ_000673"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR0822536.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHURQ_000674"
     CDS             complement(11193..11432)
                     /locus_tag="ACHURQ_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR0822537.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHURQ_000675"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHURQ_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR0822538.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHURQ_000676"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHURQ_000676"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR0822539.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHURQ_000677"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHURQ_000677"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR0822540.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHURQ_000678"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHURQ_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR0822541.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHURQ_000679"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHURQ_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHURQ_000680"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHURQ_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR0822542.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHURQ_000681"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHURQ_000681"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR0822543.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHURQ_000682"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHURQ_000682"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR0822544.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22745)
                     /locus_tag="ACHURQ_000683"
     CDS             complement(21570..22745)
                     /locus_tag="ACHURQ_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0822545.1"
                     /translation="MKINYPLLALALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAA
                     GMLISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSR
                     ILTSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGW
                     RMSFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGA
                     MFTLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLL
                     LLMVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIG
                     AFNLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22911..23000)
                     /gene="cydH"
                     /locus_tag="ACHURQ_000684"
                     /gene_synonym="ynhF"
     CDS             complement(22911..23000)
                     /gene="cydH"
                     /locus_tag="ACHURQ_000684"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR0822546.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23299..24324
                     /gene="purR"
                     /locus_tag="ACHURQ_000685"
     CDS             23299..24324
                     /gene="purR"
                     /locus_tag="ACHURQ_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR0822547.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24321..25253)
                     /gene="punR"
                     /locus_tag="ACHURQ_000686"
     CDS             complement(24321..25253)
                     /gene="punR"
                     /locus_tag="ACHURQ_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR0822548.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25366..26577
                     /gene="punC"
                     /locus_tag="ACHURQ_000687"
     CDS             25366..26577
                     /gene="punC"
                     /locus_tag="ACHURQ_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR0822549.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYCGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26868..28016
                     /gene="cfa"
                     /locus_tag="ACHURQ_000688"
     CDS             26868..28016
                     /gene="cfa"
                     /locus_tag="ACHURQ_000688"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR0822550.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28056..28697)
                     /gene="ribE"
                     /locus_tag="ACHURQ_000689"
     CDS             complement(28056..28697)
                     /gene="ribE"
                     /locus_tag="ACHURQ_000689"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR0822551.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28912..30285
                     /gene="mdtK"
                     /locus_tag="ACHURQ_000690"
     CDS             28912..30285
                     /gene="mdtK"
                     /locus_tag="ACHURQ_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR0822552.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30326..31582)
                     /locus_tag="ACHURQ_000691"
     CDS             complement(30326..31582)
                     /locus_tag="ACHURQ_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0822553.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31890..31966
                     /locus_tag="ACHURQ_000692"
     tRNA            31890..31966
                     /locus_tag="ACHURQ_000692"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31924..31926,aa:Val,seq:gac)
     gene            31971..32047
                     /locus_tag="ACHURQ_000693"
     tRNA            31971..32047
                     /locus_tag="ACHURQ_000693"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32005..32007,aa:Val,seq:gac)
     gene            32155..32460
                     /locus_tag="ACHURQ_000694"
     CDS             32155..32460
                     /locus_tag="ACHURQ_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR0822554.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32586..34190
                     /locus_tag="ACHURQ_000695"
     CDS             32586..34190
                     /locus_tag="ACHURQ_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR0822555.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34202..35014)
                     /gene="ydhT"
                     /locus_tag="ACHURQ_000696"
     CDS             complement(34202..35014)
                     /gene="ydhT"
                     /locus_tag="ACHURQ_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR0822556.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35018..35803)
                     /gene="phsC"
                     /locus_tag="ACHURQ_000697"
     CDS             complement(35018..35803)
                     /gene="phsC"
                     /locus_tag="ACHURQ_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR0822557.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35800..36468)
                     /locus_tag="ACHURQ_000698"
     CDS             complement(35800..36468)
                     /locus_tag="ACHURQ_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR0822558.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36532..37170)
                     /locus_tag="ACHURQ_000699"
     CDS             complement(36532..37170)
                     /locus_tag="ACHURQ_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR0822559.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37183..39285)
                     /locus_tag="ACHURQ_000700"
     CDS             complement(37183..39285)
                     /locus_tag="ACHURQ_000700"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR0822560.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="ACHURQ_000701"
     CDS             complement(39306..39932)
                     /locus_tag="ACHURQ_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR0822561.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9623 a        10544 c         9889 g        10057 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagcg ccagcaacgg atagttaatt
    22741 ttcatgcctt atctccacct cttcgcgtca ttacgcgata ttcattaaag tggcgaaagc
    22801 atgacagcaa tcacaaaaaa atgaaaataa caaaaagaga aagcactttt gccattttgc
    22861 taacaaacag gaaggagatg cgagggagaa cgcgctccct cgagaggaaa tcagtgcagc
    22921 gcggcagtca aacccacggc tacgatcaaa ccgaggacga taatcgttgt taccagtgaa
    22981 aatttaaggt cggtgctcat caagttttct ccttttttat taccacacaa aaagtgatat
    23041 tacgcatttt tacacactgt gatgaaaaaa tctcccgtca tttataatga taagtgtttt
    23101 taccacttcc ccttttcgtc aagatcggcc aaaattccac gcttacacta tttgcgtact
    23161 ggccattgac cccttcctga cgctccgtgt cgtttttccg gcgtaccgca acacttttgt
    23221 tgtgcgtaag gtgtgtaaag gcaaacgttt accttgcgat tttgcaggag ctgaagttag
    23281 ggtctggagt gaaatggaat ggcaacaata aaagatgtag cgaaacgagc aaacgtttcc
    23341 actacaactg tgtcacacgt gatcaacaaa acacgtttcg tcgctgaaga aacgcgcaac
    23401 gccgtgtggg cagcgattaa agaattacac tactccccta gcgcggtggc gcgtagcctg
    23461 aaggttaacc acaccaagtc tatcggtttg ctggcgacca gcagcgaagc ggcctatttt
    23521 gccgagatca ttgaagcagt tgaaaaaaat tgcttccaga aaggttatac cctgattctg
    23581 ggcaatgcgt ggaacaatct tgagaaacag cgggcttatc tgtcgatgat ggcgcaaaaa
    23641 cgcgtcgatg gtctgctggt gatgtgttct gagtacccag agccgttgct ggcgatgctg
    23701 gaagagtatc gccatatccc aatggtggtg atggactggg gtgaagcaaa agctgacttc
    23761 accgatgcgg tcattgataa cgcgttcgaa ggcggctaca tggccgggcg ttatctgatt
    23821 gaacgcggtc accgcgaaat cggcgttatc cccggcccgc tggaacgtaa cactggcgca
    23881 ggccgccttg tcggttttat gaaggcgatg gaagaagcaa tgatcaaggt gccggaaagc
    23941 tggattgtgc agggtgactt tgaacctgaa tccggttatc gcgccatgca gcaaatcctg
    24001 tcgcagccgc atcgccctac tgccgtcttc tgtggtggcg atatcatggc aatgggcgca
    24061 ctttgtgctg ctgatgaaat ggggctgcgc gtcccgcagg atgtttcgct aattggttat
    24121 gataacgtgc gcaacgcccg ctattttacg ccggcgctga ccacaatcca tcagccaaaa
    24181 gattcgctgg gtgaaacagc gttcaacatg ctgttggatc gtatcgtcaa caaacgtgaa
    24241 gaaccgcagt ccattgaagt gcatccgcgc ttgattgaac gccgctccgt ggctgacggc
    24301 ccgttccgcg actatcgtcg ttaatcaccc gttgcgggag tctcttccgg ctcccgcagc
    24361 cactccttat tcagcgtctc actatcgccg agatactcaa gcaaccaggt taacgcaggc
    24421 gacatatcat tttgctgcca tgtcagacaa catgccgaat ccggaaaggg gttttccagt
    24481 tcaagtgcta cccacttccc ctcattaagc cacggtttgg cgaaatgtgt tggcaccatc
    24541 cctatgcata atcctgccga gatacaggtt gccgatgatt cccagtcagg cacgacaact
    24601 cttttttggt tatccagcaa ccaggtaata cgtttaggta gcgttcgcga ggtgtcttcg
    24661 cgcaccaacg acggccagtt gcgcaacgta tcatcgctga acgggccatc catcaacgcc
    24721 agcgggtgat ggctggcaac aacgcaactc cagcttagca tccccatatc ccggaaggca
    24781 taacgaccgc ctaccggaat cgcgcgtgtt gcgccaatcg ccagttccac gcgcccgtcg
    24841 gaaagcgcat cccagacacc gttgaacact tcctgaaaga caagaagttc gacatcatca
    24901 aaatggcgat aaaaatcaac gatcatctgc cgtgtacgtt ctggcctgac aatattatcc
    24961 actgcgatag ctaactgacc gcgccagccg ttcgctatct gctgacattg ctggcgggtg
    25021 atctgcattt ttttgacaac agagcgccct tctttgagaa accacgctcc agcagcggtc
    25081 agctccacat cacggtgccg tcgttcaaag agcggcaccg ccagccactc ttccagctga
    25141 cgcacggtat agctgaccgc agaaggaacg cgatgcagct cctgtgccgc agcgctaaaa
    25201 ctaccattac gcgctaccgc atcaacaact tcgagtgaat attctgacca catagtctgc
    25261 ctgcaaaatt tttgaaacca atcatcaaat attaccgttt cacaacacta atttcactcc
    25321 ctacactttg cggcggtgtt taattgagag atttagagaa tatacatgca acctgggaaa
    25381 agatttttag tctggctggc gggtttgagc gtactcggtt ttctggcaac cgatatgtat
    25441 ctgcctgctt tcgccgccat acaggccgac ctgcaaacgc ctgcgtctgc tgtcagtgcc
    25501 agccttagtc tgttccttgc cggttttgcc gcagcccagc ttctgtgggg gccgctctcc
    25561 gaccgttatg gtcgtaaacc ggtattatta atcggtatga caatttttgc gttaggtagt
    25621 ctggggatgc tgtgggtaga aaacgccgct acgctgctgg tattgcgttt tgtacaggct
    25681 gtgggtgtct gcgccgcggc ggttatctgg caagcgttag tgacggatta ttatccttca
    25741 cagaaagtta accgaatttt tgcgaccatc ataccgctgg tgggtctatc tccggccctg
    25801 gctcctctgt taggaagctg gctgctggtc catttttcct ggcaggcgat tttcgccacc
    25861 ctgtttgcca ttaccgtggt gctgattctg cctattttct ggctcaaacc cacgacgaag
    25921 gcccgtaaca atagtcagga tggtctgacc tttaccgacc tgctacgttc taaaacctat
    25981 tgcggcaacg tgctgatata cgcggcctgt tcagccagtt tttttgcatg gctgaccggt
    26041 tcaccgttca tccttagtga aatgggctac agcccggcag ttattggttt aagttatgtc
    26101 ccgcaaacta tcgcgtttct gattggtggt tatggctgtc gcgccgcgct gcagaaatgg
    26161 caaggcaagc agttattacc gtggttgctg gtgctgtttg ctgtcagcgt cattgcgacc
    26221 tgggctgcgg gcttcattag ccatgtgtcg ctggtcgaaa tcctgatccc attctgtgtg
    26281 atggcgattg ccaatggcgc gatctaccct attgttgtcg cccaggcgct gcgtcccttc
    26341 ccacacgcaa ctggtcgcgc cgcagcgttg cagaacactc tacaactggg tctgtgcttc
    26401 ctcgcaagtc tggtagtttc ctggcttatt agtatcagca cgccattgct caccaccacc
    26461 agcgtgatgt tatcaacagt agtgctggtc gcgctgggtt acatgatgca acgttgtgaa
    26521 gaagctggct gccagaatca tggcaatgcc gaagtcgctc atagcgaatc acactgatct
    26581 atatcgatat acttatactt aggctgctaa caaaattttg ttgtatgatt gaaattagcg
    26641 gcctatacta atttcgagtt gttaaagcta cgataaatat tatgttttta cggggacagg
    26701 atcgttcccg actcactatg gatggtcatt tcggcaaggg ttcctccttt ccctctgttc
    26761 tacgtcggat tatagactcg cggttttttc tgcgagattt ctcacaaagc ccaaaaagcg
    26821 tctacgctgt tttaaggttc tgatcaccga ccagtgatgg agaaactatg agttcatcgt
    26881 gtatagaaga agtcagtgta ccggatgaca actggtaccg tatcgccaac gaattactta
    26941 gccgtgccgg tatagccatt aacggttctg ccccggcgga tattcgtgtg aaaaaccccg
    27001 atttttttaa acgcgttctg caagaaggct ctttggggtt aggcgaaagt tatatggatg
    27061 gctggtggga atgtgaccga ctggatatgt tttttagcaa agtcttacgc gcaggtctcg
    27121 agaaccaact cccccatcat ttcaaagaca cgctgcgtat tgccggcgct cgtctcttca
    27181 atctgcagag taaaaaacgt gcctggatag tcggcaagga gcattacgat ttgggtaatg
    27241 acttgttcag ccgcatgctt gatcccttca tgcaatattc ctgcgcttac tggaaagatg
    27301 ccgataatct ggaatctgcc cagcaggcga agctcaaaat gatttgtgaa aaattgcagt
    27361 taaaacctgg gatgcgcgta ctggatattg gctgcggctg gggcggactg gcacactaca
    27421 tggcatctaa ttatgacgta agcgtggtgg gcgtcaccat ttctgccgaa cagcaaaaaa
    27481 tggctcagga acgctgtgaa ggcctggatg tcaccatttt gctgcaagat tatcgtgacc
    27541 tgaacgacca gtttgatcgt attgtttctg tggggatgtt cgagcacgtc ggaccgaaaa
    27601 attacgatac ctattttgcg gtggtggatc gtaatttgaa accggaaggc atattcctgc
    27661 tccatactat cggttcgaaa aaaaccgatc tgaatgttga tccctggatt aataaatata
    27721 tttttccgaa cggttgcctg ccctctgtac gccagattgc tcagtccagc gaaccccact
    27781 ttgtgatgga agactggcat aacttcggtg ctgattacga tactacgttg atggcgtggt
    27841 atgaacgatt cctcgccgca tggccagaaa ttgcggataa ctacagtgaa cgctttaaac
    27901 ggatgtttac ctattatctg aatgcctgcg caggtgcttt ccgcgcccgt gatattcagc
    27961 tctggcaggt cgtgttctca cgcggtgttg aaaacggcct tcgagtcgct cgctaaaggc
    28021 tattctatcg ccccctctcc gggggcgatt tcagatcagg cttctgtacc tggttgattc
    28081 atggcatttt ctcgtgccgc cagcacacgt tctaccgtat ctaccactgc ctgagtttgt
    28141 ggatcaattt caatgttgac gcgtgcgccg agttttttct tcccaagagt cgtgcgttcc
    28201 agagtttccg gaattaaatg gacgcaaaaa cgcgttggcg tgacttcgcc gacggtcagg
    28261 ctaataccgt cgatgccaat aaatcctttg tacaggatat atttcatcaa ctgactatcc
    28321 tggactttaa accagatctg gcgattattt tccgaggtta atattttcgc cacttcagcg
    28381 gtggtcataa tatgacctga cattaagtgt ccgccaattt catcgctgaa ttttgccgca
    28441 cgctcaacgt ttacccaatc ccccactttt aaatcgccaa gattggtaat gcgtaacgtt
    28501 tctttcatca ggtcaaaact gacatggttg ccgttaattt ccgtcacggt caggcagcaa
    28561 ccgttatgcg ccacggaagc acctgtttcc aggccgtcca gcatgtggtc gggtaactcc
    28621 accacatgcg tacgaaaatt tggtttctcg tcaatcgaca ccagttttgc ggtgccctgt
    28681 acaatccccg taaacatact tacaactcct gaaatcagtt aagacattct gttcagcaca
    28741 atagcaggtg gaaaacgccc ttaccagtga aggggtaaga atgactattt tttcactgga
    28801 gaattaataa atcctcgcta caatagactg aatttcccct gcttcttctt tttgctgccc
    28861 attcaggcgg ctttttagtc tctcatataa ctacaaataa aaggtgttca cgtgcagaag
    28921 tatatcagtg aagcgcgtct gttattagca ttagcaatcc cggtgattct cgcgcaaatc
    28981 gcccaaactg cgatgggttt tgtcgatacc gtgatggcgg gcggctatag tgccaccgac
    29041 atggcggcgg tcgctatcgg tacttctatc tggcttccgg cgatcctctt tggtcacgga
    29101 ctgctgctgg cattaacgcc ggttatcgcg caattaaatg gttccggtcg acgtgagcgc
    29161 attgcgcatc aggtgcgaca aggtttctgg ctggcaggtt ttgtttccgt tctcattatg
    29221 ctggtgctgt ggaatgcagg ttacattatc cgctccatgg aaaacatcga tccggctctg
    29281 gcggacaaag ccgtgggtta tctgcgtgcg ttgttgtggg gcgcgccagg ctatcttttc
    29341 ttccaggttg cccgtaacca gtgtgaaggt ctggcaaaaa ccaagccggg tatggtaatg
    29401 ggctttatcg gcctgctggt gaacattccg gtgaactata tctttattta tggtcatttc
    29461 ggtatgcctg agctcggtgg cgttggttgt ggcgtggcta ctgcggcggt gtattgggtt
    29521 atgttccttg ccatggtttc ttacattaaa cgcgcccgct ccatgcgcga tatccgtaac
    29581 aaaaaaggca ccgcaaaacc cgatcctgcg gttatgaaac gactgattca actcggtttg
    29641 ccgattgcgc tggcactatt ctttgaagtg acactgtttg ccgtcgtggc tctgttagtg
    29701 tctccgctcg gtattgttga tgtcgcagga caccagattg ccctgaactt tagttcacta
    29761 atgttcgtgc ttccaatgtc gctggcggca gcggtaacta tccgcgtagg ttatcgtctg
    29821 ggtcagggct caacgctgga tgcgcaaacc gctgctcgga ccgggcttat ggtgggtgtc
    29881 tgtatggcaa ccctgacggc cattttcacg gtttcactgc gggagcaaat cgccctgttg
    29941 tacaacgaca atcccgaggt tgtaacgctg gctgcgcatt tgatgttgct ggcggcggta
    30001 tatcagattt ctgactcaat ccaggtgatt ggcagtggga ttttgcgtgg ttataaagat
    30061 acgcgttcca ttttctatat cacctttaca gcttactggg tgctgggctt gccaagcggc
    30121 tatattctgg cactgaccga tctggtcgtt gaacctatgg ggccagcagg cttctggata
    30181 ggctttatta tcggcctgac atcggcagcc attatgatga tgctgcgtat gcggttcctg
    30241 caacgtatgc cgtcagccat cattctgcaa cgagcgtccc gctaataaag acaaggcgca
    30301 accttcacgg gttgcgcctg tgtttttacg caggctggag cgttgcgcca atcccgtctt
    30361 cgtctggctg taatttcaga gcgttacaca gagtttgccg actggcatct gttatctcaa
    30421 cgcgtatatc cccgttaact tcccctttca cattgatgaa gtcaggtgaa atttctttcg
    30481 cactggtttg caggcaaatc gctccctctc cgcgcaggcg tttcactttc agttgcacat
    30541 gcgttgccgc accagtgatc agccacagtt ttccgccatt cagattgatg ttaccaacaa
    30601 tgaaagtgac gctgtcacct tttaccgtac tgccgtcgag aatcaacaca ccgccctgct
    30661 gtacagtaat cgttgattgt tgcagctctg cgccataacc aataatcatt gaagcgccgt
    30721 ccagtaatgc aacactcccg gtcagttcaa ccggtgcgcg atcttcctct acgacaaaat
    30781 tttcgtccgc gctggtgggt gacaacagga tcactgttcc accttgtatt accgcatttt
    30841 ccagcgtacc ggcatgcact tcagcccgcc cccccacatt caccgacagg ttttgcgtct
    30901 taccggaact gtagagctga aggccatccg tccccagacg ataaatggat ccattctcaa
    30961 caatagtgtc agagtcttca ccaccgagat tattcatggt gccaccagca agcaacgtat
    31021 cactggcttt cccggccagc ataaaaacgc cgccctgatt aatctcacaa ttgctggctt
    31081 ctccacttgg atacaccaac aattcaccac cttcatctac cacgaccgct gaggttgtcc
    31141 cattaaccaa caggccgccc tcttgatcga gaatgatttt ttccgcgcga tgtccttcca
    31201 gtacacgcag gttaccgcca ttttccagca acaaaccgca ggcataaccc tgatcaacgc
    31261 tgaattcacc ttcgggatgg cggccattaa ccgtagcgag cgtagagaga ctaattgcgc
    31321 ctccctcgga ctgaacaata tctgtggcaa tggcaccgtc gctgaccgat agcgtgccat
    31381 tgttaccgta ctgtgtgcat ttggcgatcc caccggttgc cactttcaac cagccagccc
    31441 ccgtgaccac ggtattttcg gctcttccgc cagcttcaac aatcaactcg ccttcagtaa
    31501 gttgcgtcgc gccaatgacc tcgccggtct taacaatttg cacattcccg tcgctcatca
    31561 agtttttcgc atcagatccc atgatttatt cctttgctgc atctgtgtgc ctttattgct
    31621 acctaagtgt aaaggctacg gaggatttat ccacgacaga tttgagatgg tggcaaacaa
    31681 ttctgtttaa actctgatac acgaattatt gggttgtatc agatgtaaat gcgatcctga
    31741 ataaaaatcg cccttgcaaa tcaacaaaat atagccaatt tgcttaaaag ctctgcaaac
    31801 ggtcgaaata gcgcagaaaa ttacgttttg cctcttgcca cctttccact ctgccgctaa
    31861 tatttgtccc cgttgtcacc tacaacgttg cgttcatagc tcagttggtt agagcaccac
    31921 cttgacatgg tgggggtcgt tggttcgagt ccaattgaac gcaccatcct gcgtccgtag
    31981 ctcagttggt tagagcacca ccttgacatg gtgggggtcg gtggttcgag tccactcgga
    32041 cgcaccagat tttcttaatc tggtcttttc ctttttccct ctgtttattc tctgtatcca
    32101 atacgttaaa agatttacac tgtcttcgta tgcgttatca gaaggagaat cgctatggca
    32161 actttgttac aacttcattt tgcttttaat ggcccatttg gcgacgcaat ggctgagcag
    32221 cttaaaccac ttgctgagtc gattaatcag gaacctggtt ttctgtggaa ggtatggaca
    32281 gaaagtgaaa agaaccacga agccggtggg atctaccttt tcactgatga aaaaagcgcc
    32341 cttgcctatc tggaaaaaca tactgcccga ctgaaaaatc tcggcgttga ggaagttgtc
    32401 gccaaagttt tcgatgtcaa tgagccactt agtcaaatca atcaggcaaa actcgcctga
    32461 cagaatttaa tcaagggcgg ttagcgccct tttcatccct gtctgaaatt tctcaaattc
    32521 taaaaatctc aaccaaactt atctgataac actaaattcg aaagaatgcg tacaggtaag
    32581 taacaatgaa aaaaattgct attgtgggtg ccgggcctac ggggatctac accttattct
    32641 cgcttctaca gcaacaaact ccactttcta tttctatctt cgagcaggct gacgaggccg
    32701 gtgtcgggat gccatacagt gatgaggaaa actcaaaaat gatgctggca aatattgcca
    32761 gtattgaaat accgccgatt aattgtacgt atctcgaatg gctacaaaag caagaagcca
    32821 gccatctcca gcgttatggc gttaaaaaag aaaccttgca cgatcgtcag tttttaccgc
    32881 gaattctgct gggcgaatat ttccgcgatc aatttttacg attagtagac caggcacgac
    32941 agcaaaaatt tgcagtggct gtttatgaat catgccaggt taccgatctg caaattacaa
    33001 atgctggcgt catgctcgct acaaatcagg atttacccag cgagacgttt gatttagcgg
    33061 tgattgccac gggtcacgtc tggcctgatg aagaagaagc aaccagaacg tattttccca
    33121 gcccgtggtc aggcctgatg gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa
    33181 catccttgag cggactggat gcggcaatgg cagtggctat tcagcatggt tcgttcattg
    33241 aagatgataa acaacacgtc gtttttcacc gcgataacgc aagtgaaaag ctaaatatca
    33301 cgttgatgtc gcgcacgggt attttacccg aagccgattt ctattgccct attccctacg
    33361 agcccttaca catcgtcacc gatcaggcat taaatgctga gattcaaaaa ggcgaatatg
    33421 gccttttgga tcgggtattt agattgatag tagaggaaat caagtttgct gatccagact
    33481 ggagccaacg catagcctta gagagcctga atgtcgattc ctttgctcaa gcctggtttg
    33541 ccgagcgcaa acaacgcgac ccatttgact gggcagaaaa aaatctccag gaagtcgaac
    33601 gcaataaacg agaaaaacat actgttccct ggcgttatgt cattctgcgc ctgcatgaag
    33661 ccgtacagga aattgttcca catctgaatg aacacgacca taaacggttc agtaaaggcc
    33721 ttgcccgggt tttcatcgat aattatgcgg caatcccttc agagtctatt cgtcggctgc
    33781 tggccttacg tgaagcgggg atcattcata ttctcgccct cggtgaagac tacaaaatgg
    33841 aaattaatga gtcgcgcacc gtcctgaaaa cggaagacaa cagctactcg tttgacgttt
    33901 ttattgatgc ccgcggacag cgtccgctta aagtgaaaga tattcctttc cctgggctac
    33961 gcgagcaatt acagaaaaca ggggatgaaa tccctgatgt tggcgaagat tatacgttac
    34021 agcaacccga agatattcgt ggacgcgtag cgttcggcgc gttgccctgg ttgatgcacg
    34081 accagccttt cgttcaggga cttacggcat gtgcagaaat tggtgaggcg atggctcggg
    34141 cggtcgtaaa gcctgcatcc cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct
    34201 atcagcactc aacaaactga tgctgttcat taagacgata gcggactccg gttcgaatat
    34261 tgttttcacc ttcaatggcg acggcgaagc gaactctttc gccgtcatga tatgccagtg
    34321 cggcgcttcc ccctggcccg agaataattg agtcgaccac gcccgtgctc actaccgttg
    34381 aattttcacc gctggcaatg atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg
    34441 cgccaaagct ggcgatttgc accaggtcac cattactggc gacatgacac cgttcaccca
    34501 atgtacaaac gcgtactcgc attccggtat tcgcaatccg actactatct ccagcgctgc
    34561 ttatccgcga agaattaccg gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt
    34621 gactgttaaa gccgacactt ccaatccgca cgctataacc catactggca atttgcgcgg
    34681 catacccagc ggttacgatt cgggcgttat cctcacaaca gccgatgcga gcatgatcgt
    34741 cgctgcgggc aaactgactg caaaatggta tctctccggg gtctgtggcc agtttttgca
    34801 tagaggaaac ttcctgatgc gcgaagtttt catccgccag ccattttttc cacgcatact
    34861 caaccagact ctccgcccag tccgtatatc cttcctcaat cagtgcatga tgaatatcgg
    34921 cataactacc gccgcgtggg aaatggcgca ggaaccagcg atacatcacc gctccgatac
    34981 gccattctcg caggtctgct cgtgtaatga tcattcgtta gtgccggtga tagccatcga
    35041 ccatgctttt aaaggtttcg tgtggcgtac gccccgtggt gcaaagataa agatgtccga
    35101 agataaaaaa gaggcttata aatgccagag caaaatgcgc ctgcaataac cagtatctta
    35161 cgccaggaaa cacatctccc acggcttgcg gatagagaca caacagcccc gtcaatagta
    35221 acaacggcag caatccacac atgacaccaa cataggcgac ctgctgtaag ggattaaatt
    35281 tagactgtgt tgttgccggg aaaggatgtt cttccccctg cataatgcca aacaaataaa
    35341 atcgtgtttg ttttgccgct cgttccagcc acccctgacg acgaatgcga tagtggtgac
    35401 cattatcccc aacggcattg atcagcacaa agccgagcca gcatgccagt aacaaaaatc
    35461 cgcaaacttc atgcaccgca accagacttt taaccgcagt tgcgcccacc atcgcaaagt
    35521 gatttatcag cccacttgcc agcaacaata caaagagtaa cgcattcgac cagtgccata
    35581 atctgaccgc tttgctgtaa agataaacct tctcaccatg atcggtggct gatttcttca
    35641 cgccacgagc acgaagcaag gcgtgcagcc ccaacaccaa ccacatccca accagcaata
    35701 ctccggcaat gatcaaccac accggccaaa attcgggagt gaactgtggc acatagttcg
    35761 ctaactggct ctgaaactgt tcagcatgtt gcgacgggtt catacatttt cctttttaat
    35821 caaatgttga ccgaaccggc gatacagatg cggtttcccg gcaccaagta gctgatattg
    35881 atagtattta ttttcctgca accacgcctg aatttctggg ctatcttcgc gaccaaagat
    35941 caatgcgtgt tccggacagg cgctgacgca aatgggcgga aagccttttg ccagtcggga
    36001 ctcagcgcag aaatcacatt tatctgccac tttagtgaca gggttaaggt agcgaacctg
    36061 gtacggacaa gcgccaatgc aataactgca gccgatgcac tgcgattttt ctacccgcac
    36121 gatcccctgc tcatcacgcc acgacgcccc tgtcgggcat acgtcgatgc acggagcatc
    36181 ttcgcagtgc tgacatgact ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt
    36241 gactggaatg tgtgctatcg ataaacggct tccctgagcg gggacgtggt tagttttacg
    36301 gcatgcgcgg gcgcaaatat tacagccatt gcaccgtgac tcgtcatgaa tcatggcata
    36361 ccgaacttcc ttttcttgcc tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa
    36421 gattactgtt cccatcccca gaacaaattt gcgtcgagtg aaggacatct gattatcccc
    36481 taatgcagtg gctgcaactg tccaccctat ccatgacatc tccctgaaat ttcaggggtt
    36541 ctgaaactgc tctacctcga accattcgca taagccataa gccttgccat aggtcataaa
    36601 tcgctcgcta aacaagtaca acaccgccgt cgatgactcc acctgccgta tatcggcata
    36661 ttcaggtgca acatccatcg cggcgatcgc ggcttcaatc tgcgcttcct ggaagtaata
    36721 tggcgcttgc atcagcatgg cgactttgta aggacgagga tacgtctggc actcaaaacg
    36781 aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca acttgcaaag acatcgccgc
    36841 gtagttttca ctcatggcct gcgttgagta atagtaatca tcctgacttc ccgtcataca
    36901 agcgatatcc gcgtagtctc cgttctgctt aatgctttcc agcaaaactg acagttcctc
    36961 ttctgcaacc gaatagggtg gcgagagaaa cacggtgcgc gccaccagct gcccagccgc
    37021 agaatgctgg cgaatgaact cagccaggat ttcagcaggt tccggttcgg ttaccgtatc
    37081 aacggggtga cgcatcccct gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt
    37141 cgctaacggc aattcatcct gatggttcat cttacccatt ccttacgcag gcagtagatt
    37201 gtgtgctgcc agatcggcgg caatatcttc cagccccaga cgctgcaatg tttcgcgggt
    37261 tggacaacca agctgtgggt cccagcccat ttctttgtag aacatcgtga gcgaagcatg
    37321 catatcgtca cgatccattt tgtcagtacc ttcagtaaag accgggatct gcggatcctt
    37381 gtcgaaaacc caggaacaga taagatcgtg ttcattacgc atatctttgg tttgcatcag
    37441 ttttaccgtg taggcacgat gcaacgtaaa gatacgctct gcagctaaat ctaatttttc
    37501 ctgagtcatc tcttcgccgg tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc
    37561 ccggtaatta cggcttttca gcggcgaaac ggtcattggc cagacccagt tgcacagggt
    37621 gacggcgtta tgcaaacaga cccgcaacag cgaccacttt gcatatttaa tttttgcgtc
    37681 attgattggc gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa aaagttcttt
    37741 cgccacttca cgttgcagtt tcaatggcaa gccggaacca ataaagttga tatgggtatg
    37801 cgtcatgcag tcacggttga acatacagtt aacaatggaa ccgacctgcg ctgacgcttc
    37861 attggcatgg tgaaccggat agccaaacgg cgaccagagt ttatttttcg cgtagcccca
    37921 gtactcttca cccaaattcc agcgttctgc gatggcatat gaaccatcag ccaggtgact
    37981 cagctcaccc acacgatgcg ccagacggta gtaaaaatct ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtg
//
LOCUS       DAZOZY010000015        39888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZOZY010000015 DAZOZY010000000
VERSION     DAZOZY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.015"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(126..801)
                     /locus_tag="ACHURQ_000702"
                     /pseudo
     CDS             complement(126..801)
                     /locus_tag="ACHURQ_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(946..1554)
                     /locus_tag="ACHURQ_000703"
     CDS             complement(946..1554)
                     /locus_tag="ACHURQ_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR0822562.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1738..2055)
                     /gene="metJ"
                     /locus_tag="ACHURQ_000704"
     CDS             complement(1738..2055)
                     /gene="metJ"
                     /locus_tag="ACHURQ_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR0822563.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2332..3492
                     /gene="metB"
                     /locus_tag="ACHURQ_000705"
     CDS             2332..3492
                     /gene="metB"
                     /locus_tag="ACHURQ_000705"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR0822564.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3495..5927
                     /gene="metL"
                     /locus_tag="ACHURQ_000706"
     CDS             3495..5927
                     /gene="metL"
                     /locus_tag="ACHURQ_000706"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR0822565.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6066..6200)
                     /locus_tag="ACHURQ_000707"
     CDS             complement(6066..6200)
                     /locus_tag="ACHURQ_000707"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822566.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6276..7166
                     /gene="metF"
                     /locus_tag="ACHURQ_000708"
     CDS             6276..7166
                     /gene="metF"
                     /locus_tag="ACHURQ_000708"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR0822567.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7495..9675
                     /gene="katG"
                     /locus_tag="ACHURQ_000709"
     CDS             7495..9675
                     /gene="katG"
                     /locus_tag="ACHURQ_000709"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR0822568.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9769..10674
                     /gene="yijE"
                     /locus_tag="ACHURQ_000710"
     CDS             9769..10674
                     /gene="yijE"
                     /locus_tag="ACHURQ_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR0822569.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10701..11318)
                     /locus_tag="ACHURQ_000711"
     CDS             complement(10701..11318)
                     /locus_tag="ACHURQ_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR0822570.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11592..12695)
                     /gene="gldA"
                     /locus_tag="ACHURQ_000712"
     CDS             complement(11592..12695)
                     /gene="gldA"
                     /locus_tag="ACHURQ_000712"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR0822571.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12706..13368)
                     /gene="fsa"
                     /locus_tag="ACHURQ_000713"
     CDS             complement(12706..13368)
                     /gene="fsa"
                     /locus_tag="ACHURQ_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0822572.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13380..15881)
                     /gene="ptsP"
                     /locus_tag="ACHURQ_000714"
     CDS             complement(13380..15881)
                     /gene="ptsP"
                     /locus_tag="ACHURQ_000714"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0822573.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16190..17269
                     /locus_tag="ACHURQ_000715"
     CDS             16190..17269
                     /locus_tag="ACHURQ_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR0822574.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17284..17604
                     /locus_tag="ACHURQ_000716"
                     /pseudo
     CDS             17284..17604
                     /locus_tag="ACHURQ_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17655..19951
                     /locus_tag="ACHURQ_000717"
                     /pseudo
     CDS             17655..19951
                     /locus_tag="ACHURQ_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19917..20795
                     /locus_tag="ACHURQ_000718"
     CDS             19917..20795
                     /locus_tag="ACHURQ_000718"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR0822575.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20797..21138
                     /locus_tag="ACHURQ_000719"
     CDS             20797..21138
                     /locus_tag="ACHURQ_000719"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR0822576.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21125..21976)
                     /locus_tag="ACHURQ_000720"
     CDS             complement(21125..21976)
                     /locus_tag="ACHURQ_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0822577.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22191..23924)
                     /locus_tag="ACHURQ_000721"
     CDS             complement(22191..23924)
                     /locus_tag="ACHURQ_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR0822578.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24107..26758)
                     /gene="ppc"
                     /locus_tag="ACHURQ_000722"
     CDS             complement(24107..26758)
                     /gene="ppc"
                     /locus_tag="ACHURQ_000722"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR0822579.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27110..28261)
                     /gene="argE"
                     /locus_tag="ACHURQ_000723"
     CDS             complement(27110..28261)
                     /gene="argE"
                     /locus_tag="ACHURQ_000723"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR0822580.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28415..29419
                     /gene="argC"
                     /locus_tag="ACHURQ_000724"
     CDS             28415..29419
                     /gene="argC"
                     /locus_tag="ACHURQ_000724"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR0822581.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29427..30203
                     /gene="argB"
                     /locus_tag="ACHURQ_000725"
     CDS             29427..30203
                     /gene="argB"
                     /locus_tag="ACHURQ_000725"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR0822582.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30264..31637
                     /gene="argH"
                     /locus_tag="ACHURQ_000726"
     CDS             30264..31637
                     /gene="argH"
                     /locus_tag="ACHURQ_000726"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR0822583.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31904..32821
                     /gene="oxyR"
                     /locus_tag="ACHURQ_000727"
     CDS             31904..32821
                     /gene="oxyR"
                     /locus_tag="ACHURQ_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR0822584.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32804..34204)
                     /gene="sthA"
                     /locus_tag="ACHURQ_000728"
     CDS             complement(32804..34204)
                     /gene="sthA"
                     /locus_tag="ACHURQ_000728"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR0822585.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34525..35175
                     /gene="fabR"
                     /locus_tag="ACHURQ_000729"
     CDS             34525..35175
                     /gene="fabR"
                     /locus_tag="ACHURQ_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR0822586.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35175..35534
                     /locus_tag="ACHURQ_000730"
     CDS             35175..35534
                     /locus_tag="ACHURQ_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR0822587.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35574..36674)
                     /gene="trmA"
                     /locus_tag="ACHURQ_000731"
     CDS             complement(35574..36674)
                     /gene="trmA"
                     /locus_tag="ACHURQ_000731"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR0822588.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36788..36978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37043..38887
                     /gene="btuB"
                     /locus_tag="ACHURQ_000732"
     CDS             37043..38887
                     /gene="btuB"
                     /locus_tag="ACHURQ_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR0822589.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38832..39689
                     /gene="murI"
                     /locus_tag="ACHURQ_000733"
     CDS             38832..39689
                     /gene="murI"
                     /locus_tag="ACHURQ_000733"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR0822590.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9102 a        10032 c        11003 g         9751 t
ORIGIN      
        1 aatatatgtc atttgagaaa aaagagcaga atgaggtaaa aatctttggc tgtgtattca
       61 gtctggtaat catccaagga aagcttatca gaagggtgat gactccaatt agtagaacat
      121 atattttcga taaaagcacc gatgactttt tcatgaagtt ctacagaacg cgagaaacag
      181 atggttctgc gggccagacg tttgattcgg gttctcaggg tcagattgtt acgctcaatg
      241 tgctgagtaa aaatcttacc agtcagatgc ttctccttcg gtaactctct agcgtaacta
      301 ccccattcgt cagtggtcaa catgccgatg gtaaaagggt aagcagcgcc cgcaattcac
      361 gacaggtttc gtcagtccgg gggccaaaag tgtaggccag aacaccacca gttttcgtgt
      421 tgtacacgta ccagagccag tgctgtcgcg ctttactgcc gacaaagctc cattgctgac
      481 taagtttgca gatgagtgcc acatcagcat gagcgaccgg ggatgacgtt attcttcgtg
      541 gcgagagttt tttaaagttc ggatgactgt attaatgccg attttcagcg tacgggcagt
      601 atcgcgaacc ccggcaccgt tgaaagtcat ttcagcgatt tggtctttaa ctcccggttt
      661 acggggctcg taacagtaac tgagctgaaa tacccggtga cagtcacggc cgcggaatcg
      721 ctcatgtctt tttgggttct gcccgtgacg gtaaacctga acggactggc aacgggggca
      781 atgaaggtta actgtgacca tgaaacaacc ttaaaagcga gtattatatc cttaattcaa
      841 ctaattggag gcatcaccgt ttaccggagc gacagcttgc ggaaaggtcg tgcaactaca
      901 aagcaacaaa cagactacgg cgggaattat ttttttcatc atccattacc acgaaaacag
      961 cggccactct ttggcgactg tggtgagttg tggcgcgtcg aaaacggctt gtggtgaaac
     1021 taccgtttcc tcaagcggaa tatttttgcc gtagcgttct ttgagcttag cctggaccag
     1081 cgggttgctg agatcgaact ctttcaactt ctgctgctcg cccacgcgca ttcccagcgc
     1141 gttctggtaa accttccaca ccacttccga acagtactgg cgatcgtcgc tccatgagaa
     1201 gctgaaatca tacggtttac cgagataacg ttttgccgtt tgcgccagtt tttgctgttg
     1261 ttcaacactc agtccgcctt caacgcggcg aacaacgtat ttgccctttt caccatgggc
     1321 gatccactgc ttgagcgggg tgtatttcac cgggccgacc gcttcaaaaa cgtagggctt
     1381 tttgttgcgt atcaccagca taccggtgtg gctataatcg gaatgggtcg ccagttggat
     1441 cgctttactt tgcgatgagc gagagatctg aaagatgatg tcgccggttt gtggctgcca
     1501 ggcaaaggca ggtacagaaa ccagcaggct gaggatcagc agcctgtttt tcatagttaa
     1561 acgtccatgt ataaaaagcg gtgggtcgca gacaacgtgc tcgttgttta tgccggatgc
     1621 ggcgtgaacg ccttatccgg cctacaagtt cgtgcaaatt caataaattg caatatgacg
     1681 taggcctgat aagcgtagcg catcaggcga ttccactccg cgccgctctt ttttgcttta
     1741 gtattcccac gtctccgggt taatccccat ctcacgcatg atctcttttg ccgcttccgg
     1801 gatttcgtcg ctgcgctctt tacgcagatc ggcatcatcc ggcaaaggtt gcccggtaaa
     1861 ggcatgcaga aacgcttcgc acagcagctc gctgttggta gcgtgacgca ggttgttcac
     1921 ctgacgacgc gtgcgttcat cggtgaggat ttttaacacc ttaagaggaa tggaaaccgt
     1981 aatctttttg acttgttcac tcttcttgcc gtgctcagcg tatgggctga tatattcgcc
     2041 gctccattca gccatgagat acttaatcct cttcgtcaat aaattgagac cagaccacac
     2101 aatttatgtg ggtactgacc gtaaacccgc atagtttacc gtacaggcgt taccgtgaca
     2161 tcgtgtaatg cacctgtcgg cgtgataatg catataattt taacggctat ttgggatttg
     2221 ctcaatctat acgcaaagaa gtttagatgt ccagatgtat tgacgtccat taacacaatg
     2281 tttactctgg tgcctgacat ttcaccgaca aagcccagga aacttcatca catgacgcgt
     2341 aaacaggcca ccatcgcagt gcgtagcggg ttaaatgacg acgaacagta tggttgcgtt
     2401 gtccctccca tccatctttc cagcacctat aactttaccg gatttaatga accgcgcgcg
     2461 catgattact cgcgtcgcgg caacccaacg cgcgatgtgg ttcagcgtgc gctggcagaa
     2521 ctggaaggcg gtgctggcgc agtacttact aataccggca tgtccgcgat tcacctggta
     2581 acgaccgtct ttttgaaacc tggcgatctg ctggttgcgc cgcacgactg ctacggcggt
     2641 agctatcgcc tgttcgacag tctggcgaaa cgcggttgct atcgcgtgtt gtttgttgat
     2701 caaggcgatg aacaggcatt acgggcagcg ctggcagaaa aacccaaact ggtactggta
     2761 gaaagcccaa gtaatccatt gttacgcgtc gtggatattg cgaaaatctg ccatctggca
     2821 agggaagtcg gggcggtgag cgtggtggat aacaccttct taagcccggc attacaaaat
     2881 ccgctggcat taggtgccga tctggtgttg cattcatgca cgaaatatct gaacggtcac
     2941 tctgacgtgg tggctggcgt ggtgattgct aaagacccgg acgttgtcac tgaacttgcc
     3001 tggtgggcaa acaatattgg cgtgacgggc ggcgcgtttg acagctatct gctgctacgc
     3061 gggttgcgaa cgctggtgcc gcgtatggag ctggcgcagc gcaacgcgca ggcgattgtg
     3121 aaatatctgc aaacccagcc gttggtgaaa aaactgtatc acccgtcgct gccggaaaat
     3181 caggggcatg aaattgccgc gcgtcagcaa aaaggctttg gcgcaatgtt gagttttgaa
     3241 ctggatggcg atgagcagac gctgcgtcgt ttcctgagcg ggctgtcgtt gtttacgctg
     3301 gcggaatcat tagggggagt ggaaagttta atctctcacg ccgcaaccat gacacatgca
     3361 ggcatggcac cagaagcgcg tgctgccgtc gggatctccg agacgctgct gcgtatctcc
     3421 accggtattg aagatggcga agatttaatt gccgacctgg aaaatggctt ccgggctgca
     3481 aacaaggggt aaaaatgagt gtgattgcgc aggcaggggc gaaaggtcgt cagctgcata
     3541 aatttggtgg cagtagtctg gctgatgtga agtgttattt gcgtgtcgcg ggcattatgg
     3601 cggagtactc tcagcctgac gatatgatgg tggtttccgc cgccggtagc accactaacc
     3661 agttgattaa ctggttgaaa ctaagccaga ccgatcgtct ctctgcgcat caggttcaac
     3721 aaacgctgcg tcgctatcag tgcgatctga ttagcggtct gctacccgct gaagaagccg
     3781 atagcctcat tagcgctttt gtcagcgatc tggaacgcct ggcggcgctg ctcgacagcg
     3841 gtattaacga cgcagtgtat gcggaagtgg tgggccacgg ggaagtatgg tcggcacgcc
     3901 tgatgtctgc ggtacttaat caacaagggc tgccagcggc ctggcttgat gcccgcgagt
     3961 ttttacgcgc tgaacgcgcc gcacaaccgc aggttgatga agggctttct tacccgttgc
     4021 tgcaacagct actggtgcaa catccgggca aacgtctggt ggtgaccggg tttatcagcc
     4081 gtaacaacgc cggtgaaacg gtgctgctgg ggcgtaacgg ttccgactat tccgcgacac
     4141 aaatcggtgc gctggcgggt gtttctcgtg taaccatctg gagcgacgtc gccggggtat
     4201 acagtgccga cccgcgtaaa gtgaaagatg cctgtctgct gccgttgctg cgtctggatg
     4261 aagccagcga actggctcgt ctggcggctc ccgttcttca cgcccgtact ttacagccgg
     4321 tttctggcag cgaaatcgac ctgcaactgc gctgtagcta cacgccggat caaggttcca
     4381 cgcgcattga acgcgtgctg gcctccggta ctggtgcgcg tattgtcacc agccacgatg
     4441 atgtctgttt gattgagttt caggtgcccg ccagtcagga tttcaaactg gcgcataaag
     4501 agatcgacca aatcctgaaa cgcgcgcagg tacgcccgct ggcggttggc gtacataacg
     4561 atcgccagtt gctgcaattt tgctacacct cagaagtggc cgacagtgcg ctgaaaatcc
     4621 tcgacgaagc gggattacct ggcgaactgc gcctgcgtca ggggctggcg ctggtggcga
     4681 tggtcggtgc aggcgtcacc cgtaacccgc tgcattgcca ccgcttctgg cagcaactga
     4741 aaggccagcc ggtcgaattt acctggcagt ccgatgacgg catcagcctg gtggcagtac
     4801 tgcgcaccgg cccgaccgaa agcctgattc aggggctgca tcagtccgtc ttccgcgcag
     4861 aaaaacgcat cggcctggta ttgttcggta agggcaatat cggttcccgt tggctggaac
     4921 tgttcgcccg tgagcagagc acgctttcgg cacgtaccga ctttgaattt gtgctggcag
     4981 gcgtggtgga cagccgccgc agtctgttga gctatgacgg gctggacgcc agccgcgcgt
     5041 tagccttctt caacgatgaa gcggttgagc aggatgaaga gtcgttgttc ctgtggatgc
     5101 gcgcccatcc gtatgatgat ttagtggtgc tggacgttac cgccagccag cagcttgctg
     5161 atcagtatct tgatttcgcc agccacggtt tccacgtcat cagcgccaac aaactggcgg
     5221 gagccagcga cagcaataaa tatcgccaga tccacgacgc cttcgaaaaa accgggcgtc
     5281 actggctgta caatgccacc gtcggtgcgg gcttaccgat caaccacacc gtgcgcgatc
     5341 tgatcgacag cggcgatact attttgtcga tcagcgggat cttctccggc acgctctcct
     5401 ggttgttcct gcaattcgac ggtagcgtgc cgtttaccga gctggtggat caggcgtggc
     5461 agcagggctt aaccgaacct gacccgcgtg acgatctctc tggcaaagac gtgatgcgca
     5521 agctggtgat tctggcgcgt gaagcaggtt acaacatcga accggatcag gtacgcgtgg
     5581 agtcgctggt gcctgcccat tgcgaaggcg gcagcatcga ccatttcttt gaaaatggcg
     5641 atgaacttaa cgagcagatg gtgcaacggc tggaagcggc ccgcgaaatg gggctggtgc
     5701 tgcgctacgt ggcgcgtttc gatgccaatg gcaaagcgcg tgtgggcgtg gaagcggtgc
     5761 gtgaagacca tccgctggca tcactgctgc cgtgcgataa cgtctttgcc atcgaaagcc
     5821 gctggtatcg cgataatccg ctggtgatcc gtgggcctgg cgctgggcgc gacgtcaccg
     5881 ccggggcgat tcagtcggat atcaaccggc tggcacagtt gttgtaattt cagaaattta
     5941 ataatgcccg gtactcatgt tttcgggttt atggtttcta atgaaatata ttgaattatc
     6001 ataggattag gccggattaa gcgtttacga cgaatccggc aagaagcaat aagtacatgg
     6061 ttagtttata tttgcagtcc ggtttgcttt gcataccgga ttttcttttt cttaccatcc
     6121 tgaagttttt tcatcttccc tgatttttcc tcaccatcat tggtcatttt tcggttgacg
     6181 cccttcggct tttccttcat ctttacatct ggacgtctaa acggatagat gtgcacaaca
     6241 caacatataa ctacaagcga ttgatgaggt aaggtatgag cttttttcac gccagccagc
     6301 gggatgccct gaatcagagc ctggcagaag tccaggggca gattaacgtt tcgttcgagt
     6361 ttttcccgcc gcgtaccagt gaaatggagc agaccctgtg gaactccatc gatcgcctta
     6421 gcagcctgaa accgaagttt gtatcggtga cctatggcgc gaactccggc gagcgcgacc
     6481 gtacgcacag cattattaaa ggcattaaag atcgcactgg tctggaagcg gcaccgcatc
     6541 ttacttgcat tgatgcgacg cccgacgagt tgcgcaccat tgcacgcgac tactggaata
     6601 acggtattcg tcatatcgtg gcgctgcgtg gcgatctgcc gccgggaagt ggtaagccag
     6661 aaatgtatgc ttctgacctg gtgacgctat taaaagaagt ggcagatttc gatatctccg
     6721 tggcggcgta tccggaagtt cacccggaag caaaaagcgc tcaggcggat ttgcttaatc
     6781 tgaaacgcaa agtcgatgcc ggagccaacc gcgcgattac tcagttcttc ttcgatgtcg
     6841 aaagctacct gcgttttcgt gaccgctgtg tatcggcggg cattgatgtg gaaattattc
     6901 cgggaatttt gccggtatct aactttaaac aggcgaagaa atttgccgat atgaccaacg
     6961 tgcgtattcc ggcgtggatg gcgcaaatgt tcgacggcct ggatgatgat gccgaaaccc
     7021 gcaaactggt gggcgcgaat attgccatgg atatggtgaa gattttaagc cgtgaaggag
     7081 tgaaagattt ccacttctat acgcttaacc gcgctgaaat gagttacgcg atttgccata
     7141 cgctgggtgt tcgacctggt ttataaatag tgtggctttt gtgaaaatca cacagtgatc
     7201 acaaatttta aacagagcac aaaatgctgc ctcgaaatga gggcgggaaa ataaggttgt
     7261 cagccttgtt ttctccctca ttacttgaag gatatgaagc taaaaccctt ttttataaag
     7321 catttgtccg aattcggaca taatcaaaaa agcttaatta cgatcaattt gacctacatc
     7381 tctttaacca acaatatgta agatctcaac tatcgcatcc gtggattaat tcaattataa
     7441 cttctctcta acgctgtgta tcgtaacggt aacactgtag aggggagcac attgatgagc
     7501 acgtcagacg atatccataa cacgacagcc actggcaaat gcccgttcca tcagggcggt
     7561 cacgaccaga gcgcgggggc gggcacaacc actcgcgact ggtggccaaa tcaactccgt
     7621 gttgacctgt taaaccaaca ttctaatcgt tctaacccgc tgggtgagga ctttgactac
     7681 cgcaaagaat tcagcaaatt agattactac ggcctgaaaa aagatctgaa agccctgctg
     7741 acagaatctc agccgtggtg gccagccgac tggggcagct acgccggtct gtttattcgt
     7801 atggcctggc acggcgcagg tacttaccgt tcaatcgatg gacgcggcgg cgcgggtcgt
     7861 ggtcagcaac gttttgcacc gctgaactcc tggccggata acgtaagcct cgataaagcg
     7921 cgtcgcctgt tgtggccaat caaacagaaa tatggtcaga aaatctcctg ggccgacctg
     7981 tttatcctcg caggtaacgt ggcgctggaa aactccggct tccgtacctt cggttttggt
     8041 gccggtcgtg aagacgtctg ggaaccggat ctggatgtta actggggtga tgaaaaagcc
     8101 tggctgactc accgtcatcc ggaagcgctg gcgaaagcac cgctgggtgc aaccgagatg
     8161 ggcctgattt acgttaaccc ggaaggcccg gatcacagcg gcgaaccgct ttctgcggca
     8221 gcagctatcc gcgcgacctt cggcaacatg ggcatgaacg acgaagaaac cgtggcgctg
     8281 attgcgggtg gtcatacgct gggtaaaacc cacggtgccg gtccgacatc aaacgtaggt
     8341 cctgatccag aagctgcacc gattgaagaa caaggtttag gctgggcgag cacttacggc
     8401 agcggcgttg gcgcagatgc cattacctct ggtctggaag ttgtctggac ccagacgccg
     8461 acccagtgga gcaactattt cttcgaaaac ctgttcaaat atgagtgggt acagacccgc
     8521 agtccggctg gcgcaatcca gttcgaagcg gtagacgcac cggaaattat cccggatcca
     8581 ttcgatccgt cgaagaaacg taaaccgacg atgctggtga ccgacctgac gctgcgtttt
     8641 gatcctgagt tcgagaagat ctctcgtcgt ttcctcaacg atccgcaggc gttcaacgaa
     8701 gcctttgccc gtgcctggtt caaactgacg cacagggata tggggccgaa atctcgctac
     8761 atcgggccgg aagtgccgaa agaagatctg atctggcaag atccgctgcc gcagccgatc
     8821 tacaacccga ccgagcagga cattatcgat ctgaaattcg cgattgcgga ttctggtctg
     8881 tctgttagtg agcgggtatc ggtggcctgg gcatctgctt ctaccttccg tggtggcgac
     8941 aaacgcggtg gtgccaacgg tgcgcgtctg gcattaatgc cgcagcgcga ctgggatgtg
     9001 aacgccgcag ccgttcgtgc tctgcctgtt ctggagaaaa tccagaaaga gtctggtaaa
     9061 gcctcgctgg cggatatcat cgtgctggct ggtgtggttg gtgttgagaa agccgcaagc
     9121 gccgcaggtt tgagcattca tgtaccgttt gcgccgggcc gcgttgatgc gcgtcaggat
     9181 cagactgaca ttgagatgtt cgagctgctg gagccaattg ctgacggttt ccgtaactat
     9241 cgcgctcgtc tggatgtttc caccaccgag tcactgctga tcgacaaagc ccagcaactg
     9301 accctgaccg cgccggaaat gaccgcgctg gtgggcggga tgcgcgtact gggtgccaac
     9361 ttcgatggca gcaaaaacgg cgtcttcact gaccgcgttg gcgtattgag caatgacttc
     9421 ttcgtgaact tgctggatat gcgttacgag tggaaagcga ccgacgaatc gaaagagctg
     9481 tttgaaggcc gtgaccgcga aaccggcgaa gtgaaataca ctgccagccg tgcggatctg
     9541 gtatttggtt ctaactctgt cctgcgtgcg gtggctgaag tttacgccag cagcgatgct
     9601 cacgagaagt ttgttaaaga cttcgtggcg gcatgggtga aagtgatgaa cctcgaccgt
     9661 ttcgacctgc tgtaatctga ccttcttcag cgactgcctt tcaggcagtc gctgaagttt
     9721 ctttaccggc gtatagtgtc cacaggaaaa ctacacactg gatctctcat gtctgccgca
     9781 ggaaagagca acccactggc aatcagtggc ctggttgtgc tcacacttat ctggagttat
     9841 agctggattt tcatgaagca agtcaccagc tacatcggtg ccttcgactt taccgcctta
     9901 cgctgcattt tcggcgctct cgttttattc atcgtccttt tattacgtgg tcgcggaatg
     9961 cgcccgacac cgtttaaata caccttagcc attgccctgt tacaaacctg cgggatggtt
    10021 ggcctggcgc agtgggcgct ggtcagcggc ggagcgggga aggtggcgat cctaagctat
    10081 accatgccgt tctgggtggt gattttcgcc gcgttgtttc tcggtgaacg cctgcgacgt
    10141 gggcaatatt tcgcgatact gattgccgct ttcggcttat ttttggtgtt gcagccgtgg
    10201 caactcgatt tctcttcgat gaaaagtgcc atgctggcaa tcctctccgg cgtcagttgg
    10261 ggggcgagcg cgattgttgc taaacgtctg tatgcccgtc atccgcgcgt ggatttattg
    10321 tcgttaacat cctggcagat gctctatgcg gcgctggtga tgagtgtggt cgctttactg
    10381 gtgccgcaac gtgaaattga ctggcagccc accgtgttct gggcgctggc ctacagtgcg
    10441 attctggcga cggcactggc gtggagctta tggttgtttg tattgaaaaa cttgcctgcc
    10501 agtattgcca gcttaagcac actggccgtt cccgtttgcg gcgtactctt ttcctggtgg
    10561 ctgctcggcg agaatccggg ggccgttgaa ggtagcggta ttgtgctgat tgtgatggca
    10621 ctggcgctgg tgagccgtaa gaaaaaagaa gccgttagtg taaaaaggat ctgaattttt
    10681 tcttcatgtg gggcgatcgc ttatttaaca aaataacgat aatgccccac catgcgccag
    10741 ctaaacagca catcttcttc ctgcgcacct gcgccaatgt tatgtatcac cagcggcgta
    10801 ccgtcgcggg cgaagccatc tgaaaccacc ccaatatgtg ccagcccgtt atctaatcgc
    10861 caggagacaa tatcgcccgc ttgatagtca ctggggttct tgctgatggg gcgtgtttta
    10921 tcgtggtggg taaaccaggt ttccagatta ggcacccgac ggtgatcgat gttgctgtcc
    10981 gggcgcttta actgccattt ttgcgggtac tcggcaaaat tcttcgccat atcttcgtgc
    11041 accagtttct gcaaatcgac cttctggctg cgcaatgcac ggatcaccac atcggaacat
    11101 accccgcgtt tttgcgggac atcaccgcca ggataggtaa gctgcatata cgccggatcg
    11161 taaaatagcg tgctgccgat ttgctgtctg gcaccgtctg cgatggcaag gttggtattg
    11221 gcctggattt gtaccacggt tggtggaacg gcgggagatt ttaaggagtg gctggtaaat
    11281 gccgttagca ggctgagcag cgccagtgaa gctttcatct ggttcgtccg tgaaaataag
    11341 agtcacagat tcgaccttcc gggataacga ttgttctgta gagaaaatgt ccagaagcgg
    11401 ataaaaaact cctgaacaac gtgggagttt cagattactg gcacagagtg ccggatgcgg
    11461 cgtgaacgcc ttatctgtcc taaaaaacaa gaaaattcaa tatattgcag agattgcgta
    11521 ggcctgataa gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac aagccgggag
    11581 tttggagtag gttattccca ctcttgcagg aaacgctgac cgtactggtc ggctaccagc
    11641 agagcggcgt aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat ggtttcacat
    11701 tctgcacatg ccgcttcagc cacaattcgc attttcgccg ggacatcttc tttaatatcc
    11761 agttgagcga gagttattgg caaacctacc gcatggctaa gtgcagctac ggtttcgatt
    11821 tcctcaaccg gtgcgttttc cagaaccagc tgcgtcagcg taccaaatgc cactttttca
    11881 ccgtgataat agtgatgcgc gtccgggata gcggtcaggc cgttatgcac tgcgtgcgcc
    11941 gcagccagac caccactttc aaaaccaaca ccgctcaaat aggtgttcgc ttcaatcacg
    12001 cgctccagcg ccggagtcac tacatgctgt tcggcagcaa gcatcgcttt ttcgccttct
    12061 tccagcaggg tgttgtagca cagttcagcc agtgccagcg cagcctgggt gcacttgccg
    12121 cccgccatgg tggtcgcgcc gctacgagag caggcacgcg cttcaaacca ggttgccagc
    12181 gcatcgccga tacccgccgc taacagacgt gcaggtgcgc cagcgacgat tttggtgtcg
    12241 acaatgacca tattcgggtt atttggcaac agcagatagc ggtcaaactc accctcatcg
    12301 gtgtagataa cagacaatgc gctgcacggt gcatcggtag aggcgatagt cggtgcgatc
    12361 gctaccggaa cacccatgaa atgtgccagt gctttggctg tatcgagggt ttttccgcca
    12421 ccgataccga gaattgcgcc acactgcgca gtctccgcga tgccacgcag acggtcgatc
    12481 tcattttgcg aacatccacc gccaaacggc gcaatttcta ctaccagtcc agcatcttta
    12541 aagcttttct cgacagtgga ttgagcaaaa cctaaaacaa atttgtcacc caccactaac
    12601 cagcgttctg ccagcggctt caggtattcg cccagacgat taatcacatc agcgccctgg
    12661 atgtatttac ccggtgattg aataatgcgg tccataattg ctcctttaga gatgagtagt
    12721 gccaaatgcg gcattccagt cgtgttcgaa cttctctata gctgactcta ccgcaggggt
    12781 gttgagcatt tgttgcgcta catctaaggg cagggtgatg gattcacatc ctgccagtaa
    12841 acagtccagc gcctgacgcg gcgttttaaa gctggctgcc agcaccatgc tttctggcgc
    12901 gtgcatttct aacagcgctt gcagctcctg aaccgtacga atgccgtctc cgccctgggc
    12961 atctacgcgg ttaacatacg gagcaacgta ttttgcccct gccagcgcgg ccagtaaccc
    13021 ttgtgcagca ctgtacactg cggtgccaag cgtagtaata ccctcttttt tcagtatttt
    13081 aattgctgcc agaccttcgg aagtcaccgg gattttcacc acaatacccg gaatagcgtc
    13141 gcgcaggcgc ttcgcttctt ccaccatccc ctgcgcgtcg cggctcatgg tctgagcaaa
    13201 cagaatgccc tcatcaccaa tcgctttttg cagacgcggc agcacttccc atatggactc
    13261 cttgctggca gcgataatgc tcgggttagt tgtcaccccg gcaatgggga atatgcgtgc
    13321 cagacgttcg acttctgcga cgttagcggt gtccagatac agttccatga tgtttccttt
    13381 tacagttcca gttcatgttg cagcaggctg gcgatagcgt cggcagaggc ggcgttaacc
    13441 agcgcgttac ggaattcttc gtgcatgatg cggcgagcga ggcgcgagaa aatgcgcatg
    13501 tgctgatcgc ccgcagcgtg tttgttcagg gttaacatga tgatgaattg cgcttcatca
    13561 tcaccccagc gcaccggcgc ttgcagacgc gcaacgctga tggtggattg ctcaatgtgt
    13621 tctgatttgc tgtgtggaat ggcaaaacta aagcccagac cggtagagaa aacggcctcg
    13681 cgcgcccaca agtcggcttc cagtttacgc ggatagcggc agcgccctgc cagcagcagg
    13741 ttatcggtca tgcctttgag cacttcttct ttgctgcgcc agtcgctttc cagtgtgatg
    13801 cactcggcgg tgaccagcgg tgcgtcttgt tgggtcatgc ggaattgcgc cagcaggtgc
    13861 tccacttcca gcgaagtacg gcaggccatt gcctggttga gcaacttgcg gcactcacgg
    13921 ctatcaagtt gcgccatccg cgctttcgcc gccggaattg atggtgcgct catgctgagt
    13981 tcatccagcc ctaagccgac cagcaacggc agcacggaac ctttcgctcc cagctcaccg
    14041 cacagaccaa tccatttacc ctggcgatgc accgcctgca cggcgtaatc gagcgcccgc
    14101 aagaatgccg gattcaggct gttgtagtga cgagtaacct tagcgttatc gcgatccact
    14161 gccagcagat attgcgtcag gtcattacta ccaatactaa agaaatcaat ctcttcgcag
    14221 cattgatcga tgatgaacat caccgacggc acttccagca taatgccgag ctggatcttc
    14281 tcatcaaacg gaatgtgttc gttacgtagt tgctgtttgg cttctgccag tttttctttc
    14341 acccataaga tctcttccat tgaggagatc atcgggatca tgattttcag gctgccgtga
    14401 gcggaggcgc gcaggatcga gcgtagttgt gtggtaaata acgacgcgta ttcttcataa
    14461 atacgcacgg cgcgataacc gaggaacggg tttgcctctg cgggaatgtt tagataatca
    14521 acgggtttgt cgccgccaat gtccatagtg cgcacaataa tgctgcgtcc gttggcagac
    14581 tccagcgcct gacaaaaaat gttgtacagc tcgctttcgc ccggtgcact ggtgcgatcc
    14641 atatagagca tttcagtgcg gaacaaacca acgccttccg caccattgcc gaatgcggcc
    14701 tgcgcttcca cggagtgagc gatgttagcg gcaatttcaa tgcggatacc gtcagcggta
    14761 cgggcttgtt gggtcagcca gacacgctgt tgctcacgca gggcgtcctg tacgcgggct
    14821 tcttgctgat aataacgagc tacggcttct cctggctcaa ccacaatcgc cccggcgttg
    14881 ccgtcgatat aaatcgtttg atgctgccac ggagtgaggg catcaatatc cacaccaacc
    14941 agcgtcggaa tgttgaacga acgggcaagg atcaccgtat gtgaggtggt gccgccactt
    15001 ttcagcaaca gacctttgag gtgattttta tccagttcga ggaactggct gggggtcagt
    15061 tcatcagcca tacaaatggc ggactgcgtc agtttgcccg gtgccgggaa gcgttgctca
    15121 ccgtagattt gctggagtaa ctggaagcag acgtcgcgta cgtccagggc acgttcttgc
    15181 aggtagctgc tgctggaacg ggcgaactct tcgcaaaagt gattcgcact ggcgacaatc
    15241 gcttcggcgc agcttaatcc ctcgctgacg cctgccagta agtgctgacg taaagatgta
    15301 tcgccagcca gggatcggtg agcttccagt atcgcgctgg tagcaccgtc gctatccagc
    15361 agacgaaact caatgttttt cagtaccagc gtcaggccgt tttccagtgt ggattgctcg
    15421 gcgtcaacgt ctttggctgc gggaagatta ctcagcgcat tgagatctaa agaagagatc
    15481 ggcgtcagaa tgccgcctgc actaccgctg cacacggtgc gggcgcggat aatttgcgga
    15541 ttcagattgg tcagtgaaac cggcagtggt tccagttcgt cagatttaac ttccgccagc
    15601 ggcgcatcgc agtgggggaa ttcatcgcgc agccattggc ttaaacgctg gtgcgcttcc
    15661 tgttcgtcgg ccccggaaat cagtaactgg cagttatcgc ccgccagcgt atcggtgcca
    15721 atcagcgcaa gggcgctttt ggcgttgccc ttgcggtcag tgtgcaggtt atgccactca
    15781 atttgtgatg aaaaagtatt acacagcgtt tcaacgtggc ttgccggacg cgcatgtacg
    15841 ccgttaggta gctcacaaat aaattccaca atcagggcca ttgcctctct cccggtacgg
    15901 ttttctgttt acaggataag agcgcacggc aacggcctgc catgtgacaa atctgccaaa
    15961 agctggacaa atgtaatgta accgtcaatt tgcgacgcgt ctcacaagac gccgttttgc
    16021 ggcatgcttc cggtttatcg caagttatga ggcggatcgc atttttgtac tgatattaca
    16081 aaagtccagt aaatggcctt tttatccact gtttgctccg cctgcgattg cctattgttt
    16141 cctccatcaa aaatatggac atgggctttc gcaagcccgg gagcagctta tgaatgagtt
    16201 ggtgcagatc ctgaaaaata cccgtcagca tttaatgacg ggcgtttcac acatgattcc
    16261 cttcgtggta tcgggcggta ttttgctggc ggtttccgtc atgttgtatg gcaaaggcgc
    16321 agtgccggat gccgtagccg atccaaatct gaaaaaactg tttgatatcg gcgttgcggg
    16381 cttgacgctg atggtgcctt tcctcgccgc ttacattggt tattccattg cagaacgttc
    16441 tgcgctggct ccatgcgcta tcggtacctg ggttggtaac agctttggtg cgggcttctt
    16501 tggtgcactg atcgccggga ttatcggcgg catcgtggtg cattacctga agaaaattcc
    16561 ggtgcataaa gttctgcgct cggtgatgcc tatcttcatt attcctatcg tcggcacact
    16621 gattaccgca ggcatcatga tgtggggctt gggcgagcct gtaggggcgt tgaccaacag
    16681 cctgactcag tggcttcagg ggatgcagca gggcagcatt gttatgctgg cggtgatcat
    16741 gggtctgatg ctggcgttcg atatgggcgg tccggttaac aaagtggcct atgccttcat
    16801 gctgatttgc gttgctcagg gtgtttatac cgtggtggcc atcgctgccg tgggtatttg
    16861 tatcccgccg ctggggatgg ggctggcgac gctgattggt cgtaaaaatt tctccgcaga
    16921 agagcgcgaa accggcaaag cggcactggt gatgggctgt gttggcgtta ctgaaggggc
    16981 gattcctttc gccgctgccg atccgctgcg tgtcattcct tccatcatgg tcggttcagt
    17041 ttgtggtgca gtaactgcgg cgctggtcgg tgcgcagtgc tatgcaggct ggggtggtct
    17101 gattgtgctg ccggtggttg aaggcaagct gggttatatc gcagcagtgg ctgtcggagc
    17161 agtggtgacg gctgtttgtg tgaacgtgct gaaaagtctg gcgcgtaaaa atgggtcttc
    17221 gactgatgaa aaagaagacg acctggattt ggattttgaa attaattaat tgaggaacga
    17281 gccatgacga aaattattgc agtaaccgca tgtccttcag gtgttgccca tacctatatg
    17341 gcagcagagg cgctggaaag tgccgcgaaa gcgaaaggct gggaagtgaa agtagaaacc
    17401 taggggtcaa ttggtctgga aaatgaactg actgcggaag atgtggcgag cgctgatatg
    17461 gtgattctga ccaaagatat cggcatcaag tttgaagagc gttttgctgg caagaccatc
    17521 gtgcgcgtca acatcagcga tgcagtaaaa cgtgctgacg ccattatgag caaaattgaa
    17581 gctcacctgg cgcaaaccgc ttaagttctt ctcccgctcg caagggcggg ttcgctttcc
    17641 cacaggagtt cctcatgacg aatcgtatct ctcgcctcaa aactgcactg tttgccaata
    17701 cccgtgaaat ctcgctggag cgggcgctgc tttataccgc cagccatcgg caaaccgaag
    17761 gcgaaccggt gatattgcgc cgggcgaaag caacagcgta tatccttgaa catgttgaaa
    17821 tttcgattcg tgatgaagaa ctgattgccg gtaaccgcac cgtaaaaccg cgcgccggga
    17881 ttatgtcgcc ggaaatggac ccttactggc tgctgaaaga gctggatcaa ttcccgacgc
    17941 gtccgcagga ccgctttgct atcagcgaag aagataaacg tatctaccgt gaagagttgt
    18001 tcccgtactg ggaaaaacgt tcgatgaaag atttcatcaa cgggcagatg acagatgaag
    18061 taaaagccgc gaccaacacg cagattttca gcatcaacca gacggataaa ggccaggggc
    18121 acattattat tgattaccca cgcctgttga atcacggtct gggtgagctg gtggcacaga
    18181 tgcagcaaca ttgtcagcaa cagccggaga atcactttta tcaggccgcg ttgttactgc
    18241 tggaagcctc gcagaaacac attttgcgtt acgccgaact ggcggaaacg atggcggcaa
    18301 actgcacaga tgcacagcgt cgcgaagaac tgctgactat tgcggagatc tctcgccata
    18361 acgccgaaca taagccgcag acgttctggc aggcgtgcca gttattctgg tacatgaaca
    18421 tcattctgca atacgaatcc aacgccagtt cgctgtcttt agggcgcttc gaccagtata
    18481 tgttgccgtt ctatcaggca tctttaaccc agggcgaaga tccggcgttc ctgaaagaac
    18541 tgctcgaatc tttatgggtg aaatgcaacg acatcgtgct gttgcgctcc accagcagcg
    18601 cgcgttattt cgcaggtttc ccgaccggct ataccgcact gctcggcggg ttaaccgaga
    18661 acggacgtag cgcggtgaac gtgctttcgt tcctttgcct tgacgcctat caaagcgtgc
    18721 aattaccgca accgaacctc ggcgtgcgca ctaacgcctt gatcgacacg ccgttcctga
    18781 tgaaaaccgc cgaaaccatt cgcctcggca ccggtattcc gcaaatcttt aacgatgaag
    18841 tggtggtacc agcgttcctc aaccgtggcg tttcgctgga agatgcgcgc gactattccg
    18901 tagtgggctg tgtggaatta tctattcccg gcagaaccta cggcttgcat gacatcgcga
    18961 tgtttaatct gctgaaagtg atggaaatct gcctgcatga aaatgaaggc aatgctgcgc
    19021 tgacttatga aggtttactg gagcagatcc gcgccaagat cagccactac atcaccctga
    19081 tggttgaggg cagcaatatt tgtgatatcg gccatcgcga ctgggcacct gtaccgctgc
    19141 tctcgtcttt tatcagcgat tgtctggaaa aaggtcgcga tattaccgat ggcggcgcgc
    19201 gttataactt ctccggcgta caggggatcg gtatcgccaa cctgagcgat tctctccatg
    19261 cgttgaaagg gatggttttt gagcaacagc gtttaagttt tgacgaattg ctgtcggtat
    19321 taaaagccaa ctttgcaacg ccagaaggcg aaaaagtccg tgctcgctta attaaccgct
    19381 tcgagaaata cggtaacgat atcgacgagg tggataacat cagcgccgaa ctgttacgcc
    19441 actactgcaa agaagtggaa aaataccaga acccgcgcgc ggttacttca cgccgggatc
    19501 gtataccgtt tctgctcacg tcccgttggg atcggtggtt ggcgcgacgc cagacggtcg
    19561 ttttgccggg gaacaactgg cagacggcgg cttgtcacct atgctgggcc aggacgcaca
    19621 agggccaaca gcggtactga agtcagtcag taagctcgat aatacgctgc tgtctaacgg
    19681 tacgttgctg aacgtgaaat tcactccggc gaccctggaa ggtgaagcgg gattacgcaa
    19741 actggccgac ttcttacggg cgtttaccca gcttaagttg cagcatattc agtttaacgt
    19801 ggtgaacgcc gacacgttgc gggaagcgca acagcgccca caagattatg ccgggctggt
    19861 ggtgcgcgtt gccggataca gcgccttctt tgtcgaactg tcgaaggaga tccaggatga
    19921 catcatccgc cggacagcgc atcagctgta acgttgtgga aacgcgccgc gatgatgtgg
    19981 cgcgcatttt caacattcag cgttattcac tgaatgacgg cgagggcatt cgtacggtgg
    20041 tcttttttaa aggctgtccg catctttgcc cgtggtgtgc taatccggag tcgatctccg
    20101 gcaaaatcca gacggtacgc agagaggcga aatgtctgca ctgtgcgaaa tgtttgcgtg
    20161 atgcggatga atgcccctcc ggggcgtttg aacggattgg tcgcgatatc agccttgacg
    20221 ctctggaacg ggaagtgatg aaagatgaca ttttctttcg cacgtccggc ggcggcgtca
    20281 cgctttctgg cggcgaagtg ttaatgcagg cggagtttgc tacccgtttt ttacagcgac
    20341 tgcggctgtg gggtgtgtca tgcgccattg aaactgccgg agacgcgcca gcaagcaagc
    20401 tgttaccgct ggcgaaattg tgcgatgaag tgctgttcga tttaaaaatt atggacgcga
    20461 ctcaggcgcg ggatgtggtg aagatgaacc tgccacgcgt gctggagaat ctgcgtttgc
    20521 tggtgagtga gggtgtcaac gtgatcccgc gtttaccgtt gatccctggt ttcacgctca
    20581 gcagggagaa tatgcagcag gcgctggatg tactgatccc gctgaaaatc aggcagatcc
    20641 atctgttacc gtttcatcag tacggcgaac cgaaataccg cctgctgggg aaaacatggt
    20701 cgatgaaaga ggtgcctgcg ccgtcgtcag ccgatgtgac aacgatgcgc gaaatggcag
    20761 aacgggccgg atttcaggtt accgtgggag gttaaaatgg catacctggt ggcagtaacc
    20821 gcctgcgtaa gcggcgtggc gcatacttat atggcggcgg aacggctgga aaagttgtgc
    20881 cagttagaga agtggggcgt cagcattgaa actcagggcg cgctgggaac ggagaatcgt
    20941 ttagcggacg aggatatccg tcgggcggat gttgctctgt tgattacgga tatcgagctt
    21001 gccggtgccg agcgatttga acattgccgc tatgtgcaat gcagcatcta cgcattcctg
    21061 cgtgagccgc agcgggtaat gagcgcggtg cgcaaagtgc tttctgcgcc gcagcaaacc
    21121 catcttattc tggagtagtc ggtttttctg tcagttggct gtggtactgc cggcgatatt
    21181 ccgacggcga gcgttcggtg tttttacgaa acagacggca gaagtagttg ctgtcgacaa
    21241 agccgcaggc gtgcgccact tcttttacct tcaggtcata gcctttcaat aacgtcttag
    21301 cgtgctccag tcgcgtgtga ttcaggtatt cgttaaaacc aatggccccc gttttttgaa
    21361 acaggtgcga gagatagttt ggtgaaatat agaatgcctg tgcgacagat tcgcgggtaa
    21421 gcgcggaggc atagcgttcg tcgatataat cgcgaatagc ttcaaatagt gcctggctgc
    21481 gtgaggcggt ctggatttgg ctgccgagca aatcgcggca gtggctaagc aggctggcta
    21541 cgataagcct tgccgtttgc tgctcctgcg gctgcatttg catttcattg agcgtttgta
    21601 gcagaaaaga acctatacgt gggccgcgtc gggcgacgtg ttgcttcgcc agattttgat
    21661 attgtttgcc atcccattgc acgacgctga agccgagttg ctgtttgcca aacagcacgc
    21721 taaaggtggt agcgggtgtt ttccattgtg ggaaattcca gccaccagcg gcaacgtaca
    21781 gcacatcgcc gggaactaac gttgctccag cgccggtatc aacaaactca ccttccagca
    21841 caatttccag ccgtggaaaa tcgacctgat acgcaagatc gggcaccggg ccgttactgc
    21901 tggcaaagta aatctggcgc agggacagcg ggccgttgat caggcgggag agcagatagc
    21961 tgacgtcgtg atacatgtta ttcccttagg tatgtagacg ggaaaacagt aactggcatt
    22021 attaaaaaag gccacctgaa aagtgtggcc tgaggggagt tcgatgtaaa cattgcggca
    22081 gttgtattgc cggatgtggc gtaaacgcct tatccagcct accgccgcga tctgtaggcc
    22141 ggataagacg cgtcaagcgt cgcatccggc aaatagcgcc tggctgataa ttactgatta
    22201 cctacctggt cgccatacgg cagagtgtcg taatcgatca gcgcgttttt cttgtacggg
    22261 ttaccaatcc agcgggtagt ttctttgaac tgcggattca ccactgaacg ggttggatcg
    22321 taaccgtcgt aagataagcc cgccaaatct gaccaggtgt ggatcagttc cgccaggctg
    22381 tatttacgat cgacatcctg tgagaaatca cggggatgag tcgcttgcca tttttccgac
    22441 gtccacagca ggaacggaat ggtgtacatg tggcgcgtcg ggttgtcttc gttacgcccc
    22501 tgagttttgt gcggcggcgt gtcgtaaacc tcttcaccgt ggtcagagaa gtaaaccagg
    22561 aatccgttcg ggtctgccgc tttaaagtct ttaatcaggc tggcaaccac atgatcgtta
    22621 tacaggttag cgttgtcata atcgttatat gattccagct cttctgcgct taatcctggc
    22681 ggaacatgat cggtattgcc atcaaacttg ccctgatttt ccgggtagcg gtatttgtat
    22741 ttgatatgcg tacccagcag atgaacgatg atcagtttct tcggcgcagg gtcattcagc
    22801 acatcctgga acggcttcag cacgttggtg tcgtattcac gcgcactctg cgtacgttgc
    22861 tggttcatgt agtactgctt gtcggtctgg cgcgaaaata ccgtcagcat ggtattgcgg
    22921 gcggtcatcg tctgctggtt ggtgatccag aaggttttat aacccgcctg tttcatcatg
    22981 ttcatcagcg acggctgcgt cagatacaga tccgggttct tttcattggc aaaggtcagc
    23041 gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa ctacgttatt aaacacggtc
    23101 agattcggat cggttttatg cagcgcatcc agctccggcg tggtttcacg cggataaccg
    23161 tacagactca tgcgtccgcg ctgggtcgac tcgccaatca ccagcactaa agtgcgcggt
    23221 tcgttacccg attcatcttt gaaattagcc agtggcggca aggcattatt ttcattcagt
    23281 aactttgtta gcgagtttag ttgctgacga tactgataat agccggtcag gaattgccac
    23341 ggtgcggcag gctccatacg cgaggccagg ttatccaacg ttttctcaaa cggcttgttt
    23401 ttgataaacg tattcatggc gatcggatgc agaatcaagc cataaagcag ggcaaaagag
    23461 acaacataac gccacggctt tggaatatag accgggcgca ggcgtgtcca cagcagaact
    23521 gccaccgccg tataggccag cgcgataagc acaattttca ggctgaaata ctggcttaaa
    23581 tactcgctgg cttcgttggt gttggtttcg aacatcacaa acagaacgct ctgcgagaac
    23641 tcctgcccgt agatgacgta gtagcatagc gccgccagag aggccgccca tagcaccacg
    23701 ccgattactg cggcaataat tttaatccgc ttcggaaaga ggaataccgg gatcaaccac
    23761 agcgagctga ataacagcga gtcgcgaatg ccgttggtgc cactataacc actgatgtaa
    23821 ataatggcct gtagcagagt agagaaaaac caaaagtaga gcagtgccca acccagggct
    23881 ttccagctaa aaaggggttt agcctggact tctgtggaat gcatagtaag aacctgtctt
    23941 gaaaaaaata tcgccgaatg taacgacaat tccttaagga tatctgaagg tatattcaga
    24001 atttgaataa aatgcagaca gaaatatatt gaaaacgagg gtgttagaac agaagtattt
    24061 cagaaaaccc tcgcgcaaaa gcacgagggt ttgcagaaga ggaagattag ccggtattac
    24121 gcatacctgc cgcaatcccg gcaatagtga ccattaacgc ttgttcgacg cgaggatccg
    24181 gttcctggcc ttctttttct gcctggcggg agcggtgcag caactcggcc tgcaatacgt
    24241 tcagcgggtc ggtgtaaata ttccgtagct gaatagactc tgcaatccac ggcagatcgg
    24301 ccatcagatg ggaatcgttg gcaatcgcca gcaccacttt gatgtcttct tcttgcaggt
    24361 tgcgtaactc tttacctaac ggccacagtg ctttgtctac cagacgttga tcatagtatt
    24421 ccgccaacca caggtctgct ttggcgaaga ccatctccag catgccgaga cgcgtcgaga
    24481 agaatggcca atcgcggcac atggcttcca gctcgctctg tttgccatct tcgaccactt
    24541 tttgcagcgc cgtacctgca cccagccagg cggggagcat caggcggttt tgcgtccagg
    24601 caaaaatcca cggtatggcg cgcagcgact cgacgccgcc ggttgggcga cgtttcgccg
    24661 gacgtgaacc caacggcagt ttgcccaatt cttgttccgg cgtagcggag cggaagtaag
    24721 gcacaaaatc tttgttttca cgtacgtagc cacggtagag atcgcaggag atgactgaca
    24781 gttcatccat aatgcgacgc cagctctctt tcggctcagg tggtggcagc aggttggctt
    24841 ccagaatcgc cccggtgtaa agcgacaggc tgctgacggt gatttctggc agaccatatt
    24901 taaagcggat catctcgccc tgttcagtca cgcgcaggcc acctttcagg cttcctggcg
    24961 gttgtgacag cagcgccgca tgggcaggtg cgccgccgcg accaatggaa ccgccgcgac
    25021 cgtggaacaa cgtcagctca atacccgctt tttcgcaggt tttgattaat gcatcctgtg
    25081 cctgatattg cgcccaggaa gctgccatca cgcccgcatc ttttgctgag tcggaatagc
    25141 caatcatcac catctgtttg ccctgaatca ggccgcgata ccagtcgata ttgagcagct
    25201 gggtcatgac atcgttggcg ttgttcaggt catcgagggt ttcaaacagc ggagcaaccg
    25261 gcatcgcaaa cccgataccc gcttctttca gcagcaggtg gacagccagt acgtcggacg
    25321 gcgttttcgc catcgagatc acgtaggcgg caatggagcc ttgcggcgct tcggcaatca
    25381 cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg ttgccagttg cgcggcagaa
    25441 gcggacgttt ggagttcagt tcgcggatca ggaacgcctg tttgtcggcc tctgaccagc
    25501 tttcgtagtc gccgataccg aggtagcggg tcagctcgcc cagcgcttcg gtatggcggg
    25561 tgctctcctg acggatatca atacggacca gcggtacgcc gaaacatttc acgcggcgca
    25621 gggtgtcgag cagatcgccg ttggcgataa tacccatgcc acacgcctga agtgactggt
    25681 agcaagcgta gagcggttcc cacagttctt cgttttgtgt cagcagccct tctggttttg
    25741 gcagttcttc gcctttcagg cgcgcttcca gccatgcctg tgtcgccatc aggcgagaac
    25801 gcaggttttt catcagatag cgatacggtt ctgcggcacc ttcttcgcca accagcgcca
    25861 gcagttcagg ggtcgcttca accatcgaca gttcagaaac cagaacctga atatctttca
    25921 ggaacagatc ggtggctttc cagcgactga gtagcaggac gtggcgggtg atatcggcag
    25981 tgacgttcgg gttgccgtcg cggtcaccgc ccatccacga agtaaaacgg accggaacaa
    26041 attcgacggg cagtttgtag ccgaggttct cttccagttg ttcgttcagt tcgcgcaagt
    26101 aattcggtac gccttgccac aggctgtttt ctactacggc aaagccccat ttggcttcat
    26161 ctaccgggct tggacgcagc ttacggattt catcagtatg ccatgactgg gcgattaact
    26221 ggcgcaggcg acgcatcagc tggttgtgtt cgtagtcagc gatatctttg ttatcgagct
    26281 gttttaagca ggcgttcact tccaccattt tgtggatcag tgtacgacgg gtaatttcgg
    26341 ttgggtgagc cgtgaggacc agttccagcg acagcgattc cactgctttt ttgatggtgt
    26401 cttcgctcag ttccggctgg tttttcagtt tgcgcagggt gcgggcgatc acttccgggt
    26461 tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg ctcggcggtg ttggctaggt
    26521 tcaggaactg actaaacgca cgcgcaacgg gcagcagctc gtcgttcgac aaattttgta
    26581 aggtggtgag caactcctgg cggttagcat cattgccagc gcgtgaagat ttcgacaact
    26641 tacggatagt ttctacgcgt tcaagaatgt gttctcccaa cgcatccttg atggtttctc
    26701 ccagcacttt gccgagcata ctgacattac tacgcaatgc ggaatattgt tcgttcatat
    26761 taccccagac accccatctt atcgtttgat agccctgtat ccttcacgtc gcattggcgc
    26821 gaatatgctc gggctttgct tttcgtcgtc ttttataaag ccacgtaaaa gcggtgacgt
    26881 caaatgctgc gaaatcgctt cagcaaacga ataaatagca ggaatttacg ccattaaatt
    26941 cacgacgctt taaataagcg taacttatgg aaatgttaaa aaatcgccat acataacacc
    27001 aaaggtgtag gtcggataag atgcgcaagc atcgcatccg acattattgc ggcaccggag
    27061 tttggcaacg gtgccggatg cggcgcgagc gccttatccg gcctacgttt taatgccagc
    27121 aaaaatggtg aattacctgg ataatcaact cacgggtggg tttaataaac cgcgtctcca
    27181 gatattcatc aggctgatga gcttgattga tggaacccgg accgagcacc agcgtcgggc
    27241 ataacgtttg aataaacggc gcttcggtac agtaattcac cacttcggtt tttgctccga
    27301 gcaatttctc aaccacttca accagttgat gattcggtgg gcattcatag ccagggatcg
    27361 gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg ttcgctcacc ggagccaatg
    27421 catcgttgag caaaccatta agttcattga gtgtcatgcc aggcagcgga cgaatatcca
    27481 tatgcaactc acagcaagcg caaatacggt tagaagcgtc gccaccgtga atatgcccga
    27541 ggttgagcgt agggtatggc acggtaaatg cttcgtagtg ataacgttct ttcaggttat
    27601 cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag ttcgatagcg ttaactccgc
    27661 gtgctggatc gctggagtgc cccgactggc cctgaatacg gatggcgtta gagatatgac
    27721 ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc aatgatggcg caatccgggc
    27781 gcagggcggt agtttcggca aaataacgcg ctccggccat actggtttct tcatcagcag
    27841 tcgccagaat gtagagcggt tttttcagtt tcgtgacgtc gacatcgcgt agcgtatcaa
    27901 ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa gccgtaaagc ttgccgtcat
    27961 gctccgtcag tgtaaacgga tcgcgcgtcc agcgaccgtc atcaaatggc accgtatcgg
    28021 tatgccccgc cagcaacaag ccgccagccc cctgtccgat acttgccagc atattgaatt
    28081 tgttgcgagt tcctggcaca ggctgcactt ccacattgaa gcctaaatct ttaaaccagt
    28141 ccgccagcag agtgattaaa tctgcattgc tttgatcgag tgcctcttcc gtggcgctta
    28201 ttgaaggtgt ggcaatcaga gcacggtaaa tctcgataaa tggcggtaat ttgtttttca
    28261 ttgttgacac acctctggtc atgatagtat caatattcat gcagtattta tgaataaaaa
    28321 tacactaacg ttgagcgtaa taaaacccac cagccgtaag gtgaatgttt tacgtttaac
    28381 ctggcaacca gacataagaa ggtgaatagc cccgatgttg aatacgctga ttgtgggtgc
    28441 cagcggctac gctggcgcag agctagtgac ctatgtaaat cgccatccgc atatgaacat
    28501 aaccgctttg actgtttcag cgcaaagcaa tgatgcggga aagttaatct ccgatttgca
    28561 tccgcagcta aaaggcatcg ttgagctgcc gttgcagccg atgtcggata tcagcgagtt
    28621 tagcccaggg gtggacgtag tgtttctcgc caccgcccat gaagttagcc acgatttagc
    28681 gccgcagttt cttgaagcgg gctgcgtggt gttcgacctt tccggtgcgt ttcgtgttaa
    28741 cgacgccacc ttctatgaaa aatattacgg ctttacccat caatacccgg aactgctgga
    28801 acaggcagcc tacggtctgg cggagtggtg cggtaataaa ttaaaagaag cgaatttgat
    28861 tgcggtgcca ggctgttatc cgacggcggc acagctggcg ctgaaaccgt tgattgatgc
    28921 cgatcttctt gacctcaatc agtggccggt gatcaacgcc accagcggcg tgagcggtgc
    28981 aggacgtaag gcagcgattt caaatagttt ttgtgaagtt agcctgcaac cgtatggcgt
    29041 ctttactcat cgccatcaac cagagatcgc cacacacctc ggtgctgacg ttatcttcac
    29101 cccacatctg ggcaatttcc cgcgcggcat tctcgaaacc attacctgcc gcctgaaatc
    29161 gggtgtgacc caggcgcaag tcgcgcaagt gttacagcag gcgtatgccc ataaaccgct
    29221 ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat gtcgttgggc tgccattttg
    29281 cgatatcggg tttgccgttc agggcgagca tctgattatt gtggcgaccg aagacaactt
    29341 actgaaaggc gcggcggcac aagcggtaca gtgcgccaat attcgtttcg gctatgcgga
    29401 aacgcagtct cttatttaag ggtgcaatga tgaatccatt aattatcaaa ctgggcggcg
    29461 tactgctgga tagtgaagag gcgctggaac gtctgtttag cacactggtg aattatcgtg
    29521 agtcacatca gcgtccgctg gtgattgtgc acggcggcgg ttgcgtggtg gatgagctga
    29581 tgaaagggct gaatctgccg gtgaaaaaga aaaacggcct gcgggtgacg cctgctgatc
    29641 agatagacat tatcaccgga gcactggcgg gaacggcaaa taaaaccctg ttggcatggg
    29701 cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg tgacggcgac agcgtcaaag
    29761 tgacccagct tgatgaagag ttaggtcatg ttggactggc gcagccaggt tcgcctaagc
    29821 ttatcaactc cttgctggag aacggttatc tgccggtggt cagctccatt ggcgtaacag
    29881 acgaagggca actgatgaac gtcaatgccg accaggcggc aacggcgctg gcggcaacgc
    29941 tgggcgcgga tctgattttg ctctccgacg tcagcggcat tctcgacggc aaagggcaac
    30001 gcattgccga aatgaccgcc gcgaaagcag aacaactgat tgagcagggc attattactg
    30061 acggcatgat agtgaaagtg aacgcggcgc tggatgcggc ccgcacgctg ggccgtccgg
    30121 tagatatcgc ctcctggcgt catgcggagc agcttccggc actgtttaac ggtatgccga
    30181 tgggtacgcg gattttagct taagttttgt tggccggagg cgcagctttc cggcattgaa
    30241 tttcaaaata aggaaacaga gttatggcac tttggggcgg gcgttttacc caggcagcag
    30301 atcaacggtt caaacaattc aacgactcac tgcgctttga ttaccgtctg gcggagcagg
    30361 atattgttgg ctctgtggcc tggtccaaag ccctggtcac ggtaggcgtg ttaaccgcag
    30421 aagagcaggc gcaactggaa gaggcgctga acgtgctgct ggaagatgtt cgcgccaggc
    30481 cacaacaaat ccttgaaagc gacgccgaag atatccatag ctgggtggaa ggcaaactga
    30541 tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg gcgtagtcgt aacgatcagg
    30601 tagcgactga cctgaaactg tggtgcaaag ataccgttag cgagttactg acggctaacc
    30661 gacagctgca atcggcgctg gtggaaaccg cacaaaacaa tcaggacgcg gtcatgccag
    30721 gttacactca cctgcaacgc gcccagccgg tgacgttcgc gcactggtgc ctggcctatg
    30781 ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc gcttaagcgt ctggatgtca
    30841 gcccattagg ctgtggcgcg ctggcgggaa cggcctatga aatcgaccgt gaacagttag
    30901 caggctggct gggctttgct tcggcgaccc gtaacagtct ggacagcgtt tctgaccgtg
    30961 accatgtgtt ggaactgctg tcggctgccg ctatcggcat ggtgcatctg tcgcgttttg
    31021 ctgaagatct gattttcttt aacaccggcg aagcggggtt tgtggagctt tctgaccgcg
    31081 tgacttccgg ttcatcatta atgccgcaga agaaaaaccc ggatgcgctg gagctgattc
    31141 gcggtaaatg cggtcgggtg cagggtgcgt taaccggcat gatgatgacg ctgaaaggtt
    31201 tgccgctggc ttacaacaaa gatatgcagg aagacaaaga aggtctgttc gacgcgctcg
    31261 atacctggct ggactgcctg catatggcgg cgctggtgct ggacggcatt caggtgaaac
    31321 gtccacgttg ccaggaagcg gctcagcagg gttacgccaa cgccaccgaa ctggcggatt
    31381 acctggtggc gaaaggcgta cctttccgcg aggcgcacca tatcgtgggt gaagcggtgg
    31441 tggaagccat tcgtcagggc aaaccgctgg aagatctgcc gctcgacgag ttgcagaaat
    31501 tcagcccggt gattggcgaa gatgtctatc cgattttgtc gctgcaatcg tgcctcgaca
    31561 agcgtgcggc aaaaggcggc gtctcaccgc agcaggtggc ccaagcgatt gcttttgcgc
    31621 aggcgcggtt ggggtaagaa catttatatg tacaaatttg agcctggctt atcgccgggc
    31681 ttttttatgg caaaaaaaag cggatcctgg agatccgcaa aagttcacgt tggctttagt
    31741 tattcgagtt gagaaactct cgaaacgggc agtgacttca agggttaaaa gaggtgccgc
    31801 tccgtttctg tgagcaatta tcagtcagaa tgcttgatag ggataatcgt tcattgctat
    31861 tctacctatc gccatgaact atcgtggcga tggaggatgg ataatgaata ttcgtgatct
    31921 tgagtacctg gtggcattgg ctgaacaccg ccattttcgg cgtgcggcag attcctgcca
    31981 cgttagccag ccgacgctta gcgggcaaat tcgtaagctg gaagatgagc tgggcgtgat
    32041 gttgctggag cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc tgctggtgga
    32101 tcaggcgcgt accgtgctgc gtgaggtgaa agtccttaaa gagatggcaa gccagcaggg
    32161 cgagacgatg tccggaccgc tgcatattgg tttgattccc acggttggac cgtacctgct
    32221 accgcatatt atcccgatgc tgcaccagac ctttccaaag ctggaaatgt atctgcatga
    32281 agcacagacc caccagttac tggcgcaact ggacagtggc aaactcgatt gcgtgatcct
    32341 cgcgctggtg aaagagagcg aagcattcat tgaagtgccg ttgtttgatg agccaatgtt
    32401 gctggctatc tatgaagatc acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct
    32461 ggcaggggaa aaactgctga tgctggaaga tggtcactgt ttgcgcgatc aggcaatggg
    32521 tttctgtttt gaagccgggg cggatgaaga tacacacttc cgcgcgacca gcctggagac
    32581 gctgcgcaac atggtggcgg caggtagcgg gatcacttta ctgccagcgc tggctgtgcc
    32641 gccggagcgc aaacgcgatg gggttgttta tctgccgtgc attaagccgg aaccacgccg
    32701 cactattggc ctggtttatc gtcctggctc accgctgcgc agccgctatg agcagctggc
    32761 agaggccatc cgagcaagaa tggatggcta tttcgataaa gtgttaaaac aggcggttta
    32821 aaccgtttaa cgcagctacc cgataggctt ccgccatggt cgggtagtta aaggtggtgt
    32881 tgacgaagta ctcaatagtg ttgccgccac ctttctgttc cataatcgcc tgaccgatat
    32941 gaataatttc ggcagcgcgc tcgccaaagc agtgaatacc cagaatctct tttgtttccc
    33001 gatggaacaa aattttcagc gtgcccacgt tcatgccgac gatttgtgcg cgtgccagat
    33061 gtttaaactg ggcgcggccc acttcatatg gcactttcat cgcggtcagc tgctgttcgg
    33121 ttttgcccac agagctgatt tccgggatgg tgtaaatgcc ggtagggata tcttcaatca
    33181 gatgcgcggt ggcttcgcct tttaccagcg cctgcgcggc aatgcgcccc tggtcatagg
    33241 ccgctgatgc caggctcgga taaccaatca cgtcgcccac cgcgtaaacg tgcggctgtg
    33301 cggtctgata catgctgttg accttcagct gtccgcgact gtcagtttcc agcccaatgt
    33361 tctgtaacgc cagcgaatcg gtattaccgg tacgaccgtt ggcatagagc aggcagtcag
    33421 ctttcagttt tttacccgac ttcagatgca tgatcacacc gtcgtcacag ccttcgatct
    33481 tctcgtactc ttcgttgtga cgaatcacta cgccactgtt ccagaagtga taggagagag
    33541 aatctgacat ctcttgatcg agaaatgcca gcaggcgatc gcgggtgttg atcagatcca
    33601 cttttacatc cataccgcgg aagatcgacg catattcaca gccgatcact ccagcaccat
    33661 agataagtac atggcgcggt tcgtggtgca tgctaagaat tgagtcgctg tcgtaaatgc
    33721 gtggatgggt gaaatcaaca tctgttggat gatatggacg agagccgcag gcaataacaa
    33781 atttttcagc ggttagtgtt tcgacgctgc cgtccgggca atccagcgcc aacgtatgct
    33841 cgtcaacaaa gcgagcgttc ccctgcaata tttcacagtg attacgttcg taaaatccct
    33901 gacgcatgcg cgtttgttga ttaatcacgt tatcggcatg gttaaggata tcggcaaaag
    33961 aagagcggag cagtcgcgaa tggtcgctgt atagtgggtt ttgattgaat tctataatgc
    34021 ggctgacggc gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc
    34081 caacattttg ataacgctcg ataactgcga cgcgcacacc ttgcttaacc aggcccattg
    34141 cagcgccttc gccgccgggg ccggaaccta ttactatggc atcgtaatcg taggaatgtg
    34201 gcatggtagg gcttacctgt tcttatacat aaaagcaaca gaatggtaac attctatcgc
    34261 gggtaagcca attgatcccc gtcatttatc tggctatatc ctgagcggcc tttgctttgt
    34321 ctgtttctta cttttgccct gacgttttat tggattttta tcgacgatac tctccgttta
    34381 agcggcaggt ttccgctgta cgtaaaagaa ccggccagta aatatgtttt attgcgttac
    34441 cgttcattca caatactgga gcaatccagt atgttcattc tctggtatag tgccagcagt
    34501 acttttggca aggattcaga catcgtgatg ggcataagag cgcaacaaaa agaaaaaacc
    34561 cgccgttcgc tggtggaagc cgcatttagc caattaagtg ctgaacgcag cttcgccagc
    34621 ctgagtttgc gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt ttatcggcat
    34681 ttccgcgacg tagacgaact gggtctgacc atggttgatg agagcggttt aatgctacgc
    34741 caactcatgc gccaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat ccgcacctcg
    34801 gtctccacat ttatggagtt catcggtaat aatcctaacg ccttccggtt attattgcgg
    34861 gaacgctccg gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat tcagcacttc
    34921 attgcggaac ttgcggacta tctggaactc gaaaaccata tgccgcgtgc gtttactgaa
    34981 gcgcaagccg aagcaatggt gacaattgtc ttcagtgcgg gtgccgaggc gttggacgtc
    35041 ggcgtcgaac aacgtcggca attagaagag cgactggtac tgcaactgcg aatgatttcg
    35101 aaaggggctt attactggta tcgccgtgaa caagagaaaa ccgcaattat tccgggaaat
    35161 gtgaaggacg agtaatgaaa caagcaaatc aagatagagg tacgctgctg ctggcgttag
    35221 ttgctggctt atcgattaat ggtactttcg cagcgctgtt tagctccatt gtgccatttt
    35281 ctgtattccc gattatttcc ctggtgctga cggtttactg cctgcatcaa cgttatctta
    35341 atcgcactat gccggtaggc ttgccgggtc tggcagctgc ctgttttatt ctcggcgtac
    35401 tgctgtacag cacggtagtt cgtgcggaat atccggatat cggctctaac ttcttcccgg
    35461 cagtactctc cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac cgtaagcagg
    35521 aagttgctga gtaatcggta ttatgccgga tgctggcgca tccggcatgg gttttacttc
    35581 gcggtcagta atacgccgca ctccatatgg tgcgtgtagg ggaactgatc aaacagagcc
    35641 agacgttcga ccttgtgcgt ctggcttaat gtttccagat tcttgcataa cgtttccggg
    35701 ttacaggaga tgtacaaaat acacggatac gcctgcacca ttttctcggt ttcactgtcc
    35761 agaccgctgc gcggtgggtc gacaaaaatg gtttcgcact gataactctt taagtcgatc
    35821 ccttgcaggc ggttaaactc gcgcacacca ttcatcgcct gagtaaattc ttctgccgcc
    35881 atacgaataa tttgtacgtt atcaatatgg ttagctgcga tgttgtattg cgcagcagca
    35941 accgacggct tagcgatttc ggtggctaat acccgatcaa aattgcgcgc cagcgctaat
    36001 gaaaagttac cgttgccgca gtacagctcc agtaaatcgc cttttgagcc tttggttacg
    36061 tccagcgccc attccagcat ctgaatattc atcgccgcgt tcggctgggt aaagctgttt
    36121 tctacctgac ggtagatcat ctctttccct gcgaccggca gacgctcgtc gatgtaatct
    36181 tgatccagct cgattttggt tttcgttgcc cgaccaatca gatgcacatt cagattctgc
    36241 gcgcgcagtg catcgcgcag ggcctccgct tcctgacgcc actcatcatc cagcttctta
    36301 tggtatagca gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc aatctggaac
    36361 aacttgtggc gcagaacggg attattacgc acacccgcaa tcatcgccgt catcaactgg
    36421 ttgataagtt cactggcggc ggggaagcta tccacgcgga tacggctttt ggtttgttga
    36481 tcgaaaatga tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc cgcacgcatc
    36541 cggtaatgac tgaccggcga gcgaaacact tccggaacca ggtcagaaaa cggtgccatc
    36601 atactttgca aacgtaccac tttttcggct aactgcgctt catactgttc tgttggaagg
    36661 tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt agggattgct
    36721 catcagatgt ccagatcttg atgaattcct atttgtgagc tacgtctgga cagtaacttg
    36781 ttacaacctg tagcatccac ttgtcggtcc tgtgagttaa tagggaatcc agtgcgaatc
    36841 tggagctgac gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg acactgccat
    36901 ccggtgggaa gtcatcatct cttagtatct tagatacccc tccaagcccg aagacctgcc
    36961 ggccaacgtc gcatctggtt ctcatcatcg cgtaatattg atgaaacctg cggcatcctt
    37021 cttctattgt ggatgcttta caatgattaa aaaagcttcg ctgctgacgg cgtgttctgt
    37081 cacagccttt tccgcttggg cacaggatac cagcccggat actctcgtcg ttactgctaa
    37141 ccgttttgaa cagccgcgca gcactgtgct tgcaccaacc accgttgtga cccgtcagga
    37201 tatcgaccgc tggcagtcga cctcggtcaa tgatgtgctg cgccgtcttc cgggcgtcga
    37261 tatcacccaa aacggcggtt caggtcagct ctcatctatt tttattcgcg gtacaaatgc
    37321 cagtcatgtg ttggtgttaa ttgatggcgt acgcctgaat ctggcggggg tgagtggttc
    37381 tgccgacctt agccagttcc ctattgcgct tgtccagcgt gttgaatata tccgtgggcc
    37441 gcgctccgct gtttatggtt ccgatgcaat aggcggggtg gtgaatatca tcacgacgcg
    37501 cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc aatagttatc agaactatga
    37561 tgtctctacg cagcaacaac tgggggataa gacacgggta acgctgttgg gcgattatgc
    37621 ccatactcat ggttatgatg ttgttgccta tggtaataca ggaacgcaag cgcagacaga
    37681 taacgatggt tttttaagta aaacgcttta tggcgcgctg gagcataact ttactgatgc
    37741 ctggagcggc tttgtgcgcg gctatggcta tgataaccgt accaattatg acgcgtatta
    37801 ttctcccggt tcaccgttgc tcgatacccg taaactctat agccaaagtt gggacgccgg
    37861 gctgcgctat aacggcgaac tgattaaatc acaactcatt accagctata gccatagcaa
    37921 agattacaac tacgatcccc attatggtcg ttatgattcg tcggcgacgc tcgatgagat
    37981 gaagcaatac accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta gtattggtgc
    38041 gggtgtcgac tggcagaaac agactacgac gccgggtaca ggttatgttg aggatggata
    38101 tgatcaacgt aataccggca tctatctgac cgggctgcaa caagtcggcg attttacctt
    38161 tgaaggcgca gcacgcagtg acgataactc acagtttggt cgtcatggaa cctggcaaac
    38221 cagcgccggt tgggaattca tcgaaggtta tcgcttcatt gcttcctacg ggacatctta
    38281 taaggcacca aatctggggc aactgtatgg cttctacgga aatccgaatc tggacccgga
    38341 gaaaagcaaa cagtgggaag gcgcgtttga aggcttaacc gctggggtga actggcgtat
    38401 ttccggatat cgtaacgatg tcagtgactt gatcgattat gatgatcaca ccctgaaata
    38461 ttacaacgaa gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt ttgataccgg
    38521 accactgacg catactgtga gttatgatta tgtcgatgcg cgcaatgcga ttaccgacac
    38581 gccgttgtta cgccgtgcta aacagcaggt gaaataccag ctcgactggc agttgtatga
    38641 cttcgactgg ggtattactt atcagtattt aggcactcgc tatgataagg attactcatc
    38701 ttatccttat caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg cggttgcgta
    38761 tccggtcacc tctcacctga cagttcgtgg taaaatagcc aacctgttcg acaaagatta
    38821 tgagacagtc tatggctacc aaactgcagg acgggaatac accttgtctg gcagctacac
    38881 cttctgaacc acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct
    38941 atgacgagat ccggcatctc ttaccggatc tccattacat ttatgctttc gataacgtcg
    39001 ctttcccgta tggcgaaaaa agcgaagcgt ttattgttga gcgagtggtg gcaattgtca
    39061 ccgcggtgca agaacgttat ccccttgcgc tggctgtggt cgcttgcaac actgccagta
    39121 ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg
    39181 cgattaaacc tgctgcacgt ctgacggcaa atggcattgt cggattactg gcaacccgcg
    39241 gaacagttaa acgttcttat actcatgagc tgatcgcgcg tttcgctaat gaatgccaga
    39301 tagaaatgct gggctcggca gagatggttg agttggctga agcgaagcta catggcgaag
    39361 atgtttctct ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg aaagagccgc
    39421 cagataccgt tgtattgggt tgcacccatt tccctctact acaagaagaa ctgttacaag
    39481 tgctgccaga gggaacccgg ctggtggatt ctggcgcagc gattgctcgc cgaacggcct
    39541 ggttgttaga acatgaagcc ccggatgcaa aatctgccga tgcgaatatt gccttttgta
    39601 tggcaatgac gccagaagct gaacaattat tgcccgtttt acagcgttac ggcttcgaaa
    39661 cgctcgaaaa actggcagtt ttaggctgat ttggttgaat gttgcgcggt cagaaaatta
    39721 ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg
    39781 aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaatctca acagagaaaa
    39841 gcaaaaaaaa tgcttgactc tgtagcggga aagcgtatta tgcacacc
//
LOCUS       DAZOZY010000016        39409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZOZY010000016 DAZOZY010000000
VERSION     DAZOZY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.016"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHURQ_000734"
     CDS             <1..266
                     /locus_tag="ACHURQ_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR0822591.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHURQ_000735"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHURQ_000735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR0822592.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHURQ_000736"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHURQ_000736"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR0822593.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHURQ_000737"
     CDS             complement(1570..3270)
                     /locus_tag="ACHURQ_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR0822594.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHURQ_000738"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHURQ_000738"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR0822595.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHURQ_000739"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHURQ_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="ACHURQ_000740"
     CDS             4828..6600
                     /locus_tag="ACHURQ_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0822596.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVSEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHURQ_000741"
     CDS             6593..8374
                     /locus_tag="ACHURQ_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR0822597.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHURQ_000742"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHURQ_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR0822598.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHURQ_000743"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHURQ_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR0822599.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHURQ_000744"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHURQ_000744"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR0822600.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHURQ_000745"
     CDS             11336..11908
                     /locus_tag="ACHURQ_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR0822601.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHURQ_000746"
     CDS             complement(11939..12250)
                     /locus_tag="ACHURQ_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR0822602.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHURQ_000747"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHURQ_000747"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHURQ_000748"
     CDS             12629..12982
                     /locus_tag="ACHURQ_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFR0822603.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     NVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHURQ_000749"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHURQ_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHURQ_000750"
     CDS             complement(14736..15206)
                     /locus_tag="ACHURQ_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR0822604.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHURQ_000751"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHURQ_000751"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR0822605.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHURQ_000752"
     CDS             complement(16044..16262)
                     /locus_tag="ACHURQ_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR0822606.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHURQ_000753"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHURQ_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR0822607.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHURQ_000754"
     CDS             complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHURQ_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR0822608.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHURQ_000755"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHURQ_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR0822609.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="ACHURQ_000756"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="ACHURQ_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR0822610.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="ACHURQ_000757"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="ACHURQ_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR0822611.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            25913..26011
                     /locus_tag="ACHURQ_000758"
                     /pseudo
     CDS             25913..26011
                     /locus_tag="ACHURQ_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHURQ_000759"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHURQ_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR0822612.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHURQ_000760"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHURQ_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR0822613.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="ACHURQ_000761"
     CDS             26836..27213
                     /locus_tag="ACHURQ_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR0822614.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="ACHURQ_000762"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="ACHURQ_000762"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR0822615.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHURQ_000763"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHURQ_000763"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR0822616.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMPAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="ACHURQ_000763"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="ACHURQ_000764"
     CDS             30024..30353
                     /locus_tag="ACHURQ_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR0822617.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="ACHURQ_000765"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="ACHURQ_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR0822618.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="ACHURQ_000766"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="ACHURQ_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR0822619.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="ACHURQ_000767"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="ACHURQ_000767"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR0822620.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="ACHURQ_000768"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="ACHURQ_000768"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR0822621.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHURQ_000769"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHURQ_000769"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR0822622.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="ACHURQ_000770"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="ACHURQ_000770"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR0822623.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHURQ_000771"
     CDS             complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHURQ_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR0822624.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(39320..>39409)
                     /locus_tag="ACHURQ_000772"
     CDS             complement(39320..>39409)
                     /locus_tag="ACHURQ_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822625.1"
                     /translation="SIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         9529 a        10050 c        10416 g         9414 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gtcggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc aatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcaatagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atgcccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgata cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAZOZY010000017        38864 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZOZY010000017 DAZOZY010000000
VERSION     DAZOZY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.017"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            57..1106
                     /locus_tag="ACHURQ_000773"
     CDS             57..1106
                     /locus_tag="ACHURQ_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR0822626.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1180..2037
                     /gene="nfo"
                     /locus_tag="ACHURQ_000774"
     CDS             1180..2037
                     /gene="nfo"
                     /locus_tag="ACHURQ_000774"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR0822627.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2041..3129
                     /locus_tag="ACHURQ_000775"
     CDS             2041..3129
                     /locus_tag="ACHURQ_000775"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR0822628.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3185..4435)
                     /locus_tag="ACHURQ_000776"
     CDS             complement(3185..4435)
                     /locus_tag="ACHURQ_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0822629.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4535..5476)
                     /gene="rihB"
                     /locus_tag="ACHURQ_000777"
     CDS             complement(4535..5476)
                     /gene="rihB"
                     /locus_tag="ACHURQ_000777"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR0822630.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5606..6304
                     /locus_tag="ACHURQ_000778"
     CDS             5606..6304
                     /locus_tag="ACHURQ_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR0822631.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6371..7621)
                     /locus_tag="ACHURQ_000779"
     CDS             complement(6371..7621)
                     /locus_tag="ACHURQ_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR0822632.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7715..8653)
                     /gene="psuG"
                     /locus_tag="ACHURQ_000780"
     CDS             complement(7715..8653)
                     /gene="psuG"
                     /locus_tag="ACHURQ_000780"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR0822633.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8641..9582)
                     /locus_tag="ACHURQ_000781"
     CDS             complement(8641..9582)
                     /locus_tag="ACHURQ_000781"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR0822634.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(10040..11731)
                     /gene="fruA"
                     /locus_tag="ACHURQ_000782"
     CDS             complement(10040..11731)
                     /gene="fruA"
                     /locus_tag="ACHURQ_000782"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR0822635.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(11748..12686)
                     /gene="fruK"
                     /locus_tag="ACHURQ_000783"
     CDS             complement(11748..12686)
                     /gene="fruK"
                     /locus_tag="ACHURQ_000783"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR0822636.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12686..13816)
                     /gene="fruB"
                     /locus_tag="ACHURQ_000784"
     CDS             complement(12686..13816)
                     /gene="fruB"
                     /locus_tag="ACHURQ_000784"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR0822637.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14184..15365
                     /gene="setB"
                     /locus_tag="ACHURQ_000785"
     CDS             14184..15365
                     /gene="setB"
                     /locus_tag="ACHURQ_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR0822638.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15362..15616)
                     /locus_tag="ACHURQ_000786"
     CDS             complement(15362..15616)
                     /locus_tag="ACHURQ_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR0822639.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15771..16343
                     /gene="yeiP"
                     /locus_tag="ACHURQ_000787"
     CDS             15771..16343
                     /gene="yeiP"
                     /locus_tag="ACHURQ_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR0822640.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16566..18032
                     /locus_tag="ACHURQ_000788"
     CDS             16566..18032
                     /locus_tag="ACHURQ_000788"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0822641.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18150..19136
                     /gene="yeiR"
                     /locus_tag="ACHURQ_000789"
     CDS             18150..19136
                     /gene="yeiR"
                     /locus_tag="ACHURQ_000789"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR0822642.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            19175..19888
                     /gene="lpxT"
                     /locus_tag="ACHURQ_000790"
     CDS             19175..19888
                     /gene="lpxT"
                     /locus_tag="ACHURQ_000790"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR0822643.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            20300..20866
                     /gene="mepS"
                     /locus_tag="ACHURQ_000791"
     CDS             20300..20866
                     /gene="mepS"
                     /locus_tag="ACHURQ_000791"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR0822644.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21047..22603
                     /locus_tag="ACHURQ_000792"
     CDS             21047..22603
                     /locus_tag="ACHURQ_000792"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR0822645.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22685..24499
                     /locus_tag="ACHURQ_000793"
     CDS             22685..24499
                     /locus_tag="ACHURQ_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR0822646.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            24500..25594
                     /locus_tag="ACHURQ_000794"
     CDS             24500..25594
                     /locus_tag="ACHURQ_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR0822647.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25594..26619
                     /locus_tag="ACHURQ_000795"
     CDS             25594..26619
                     /locus_tag="ACHURQ_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0822648.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            26621..28210
                     /gene="yejF"
                     /locus_tag="ACHURQ_000796"
     CDS             26621..28210
                     /gene="yejF"
                     /locus_tag="ACHURQ_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR0822649.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(28214..28558)
                     /locus_tag="ACHURQ_000797"
     CDS             complement(28214..28558)
                     /locus_tag="ACHURQ_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR0822650.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28891..29948)
                     /locus_tag="ACHURQ_000798"
                     /pseudo
     CDS             complement(28891..29948)
                     /locus_tag="ACHURQ_000798"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(30039..30734)
                     /gene="rsuA"
                     /locus_tag="ACHURQ_000799"
     CDS             complement(30039..30734)
                     /gene="rsuA"
                     /locus_tag="ACHURQ_000799"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR0822651.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30884..32644
                     /locus_tag="ACHURQ_000800"
     CDS             30884..32644
                     /locus_tag="ACHURQ_000800"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR0822652.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32769..33053
                     /gene="rplY"
                     /locus_tag="ACHURQ_000801"
     CDS             32769..33053
                     /gene="rplY"
                     /locus_tag="ACHURQ_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR0822653.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33192..34199)
                     /gene="yejK"
                     /locus_tag="ACHURQ_000802"
     CDS             complement(33192..34199)
                     /gene="yejK"
                     /locus_tag="ACHURQ_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR0822654.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34381..34608
                     /locus_tag="ACHURQ_000803"
     CDS             34381..34608
                     /locus_tag="ACHURQ_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR0822655.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34628..36388
                     /gene="yejM"
                     /locus_tag="ACHURQ_000804"
     CDS             34628..36388
                     /gene="yejM"
                     /locus_tag="ACHURQ_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR0822656.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36463..36539
                     /locus_tag="ACHURQ_000805"
     tRNA            36463..36539
                     /locus_tag="ACHURQ_000805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36497..36499,aa:Pro,seq:ggg)
     gene            complement(36642..38864)
                     /gene="yejO"
                     /locus_tag="ACHURQ_000806"
                     /pseudo
     CDS             complement(36642..38864)
                     /gene="yejO"
                     /locus_tag="ACHURQ_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT         9556 a        10131 c         9423 g         9754 t
ORIGIN      
        1 aattttattt ttaaaccagt tagtcgtaac cttatgcccg gttaaaggag agggttatga
       61 cgaatatcac cttacagaaa caacatcgta cactgtggca ttttattccg gggttagccc
      121 tgagtgcagt tatcaccggg gtcgccctgt ggggtggttc cattcccgcg gttgcgggtg
      181 ccgggtttag tgccctcacc ctcgcaatct tgttggggat ggttttaggc aacaccatct
      241 atccgcacat ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa tatttattac
      301 gtctgggtat cattctttat ggcttccgtc tgacgttctc gcaaattgcc gatgtcggta
      361 tcagtgggat catcattgac gtgttgacgc tgtccagtac cttcctgctg gcttgcttcc
      421 tggggcagaa agtgtttggt ctggataagc acaccagctg gttgatcggt gccggtagca
      481 gtatctgtgg tgctgccgcg gtactggcga ctgagtcggt agtgaaagcg gaagccagta
      541 aagtaaccgt ggctgttgca accgttgtta tcttcgggac cgtcgcgatt ttcctctacc
      601 cggcgatata tccgctgatg tctcaatggt ttagtccgga aactttcggt atctacattg
      661 gttctactgt gcacgaagtg gcgcaggtgg tggcggcagg tcatgccatc agcccggatg
      721 cggaaaacgc agcagttatt tccaaaatgc tgcgcgtgat gatgctggct ccgttcctca
      781 tcctgctggc ggcgcgtgtt aaacagctgt ctggggcgaa cagcggcgag aaaagcaaaa
      841 tcactattcc gtggtttgcc atcttgttca tcgtcgttgc catctttaac tcgttccacc
      901 tgttaccgca gagcgtggtg aacatgctgg taacgctgga taccttcctg ctggcaatgg
      961 cgatggcggc gctgggtctg accactcacg tcagcgcgct gaaaaaagct ggggcgaaac
     1021 ctctgctgat ggcactggta ctgtttgcct ggctgattgt tggtggtggt gcgattaact
     1081 atgtgattca aagcgtcatc gcataaacca ctacatcttg ctcctgttaa cccgctatca
     1141 ttaccgtttt cctccagcgg gtttaacagg agtcctcgca tgaaatacat tggagcgcac
     1201 gttagtgctg ctggcggtct ggcaaatgcc gcaattcgcg ccgccgaaat cgacgcaacc
     1261 gcgtttgcct tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct cacgacgcaa
     1321 accatcgatg aattcaaagc cgcctgtgaa aaatatcact acacatcggc gcaaattctt
     1381 ccccacgaca gttatctgat taacctcgga catccggtca ctgaagctct ggaaaaatcg
     1441 cgcgatgcct ttatagatga aatgcagcgt tgcgaacaac tggggctttc tttgctcaac
     1501 ttccaccctg gcagccatct gatgcagatt tcagaagagg attgccttgc gcgtattgcc
     1561 gaatccatca acattgcgct ggataaaact caaggtgtaa cagcggtgat tgaaaacacc
     1621 gccggtcagg gcagtaactt agggtttaaa ttcgaacatc tcgcggcgat tatcgacggc
     1681 gtggaagata aatcccgcgt cggcgtctgc attgatacct gccatgcttt cgctgccggg
     1741 tatgatttgc gtactccagc cgaatgcgaa aaaacgttcg ctgattttgc ccgtattgtc
     1801 ggctttaagt atctgcgcgg gatgcacctt aacgatgcga aaagtacctt tggcagccgc
     1861 gttgaccgcc atcatagcct cggtgaaggc aatatcggtc atgatgcgtt ccgctggatc
     1921 atgcaagacg accgtttcga cggcattccg ctgatccttg aaaccatcaa cccggatatc
     1981 tgggcagaag agatcgcctg gctgaaagcg caacaaactg aaaaagcggt ggcctgatcg
     2041 atgaacgaca gggaaaagca gattcttaaa attttacggc gtaacccgct gattcaacag
     2101 aatgaaatcg ccgatattct gcaaatcagc cgctcacgtg ttgccgctca tattatggat
     2161 ttgatgcgca agggtttgat taaaggcaaa ggatatatcc ttaccgagca ggattactgt
     2221 gtggttgtgg gtgcaatcaa tatggatatt cgcggaatgg cggatatccg ttacccgcaa
     2281 gcagcctctc atccaggttc tgttcactgt tccgcaggcg gcgtcggacg taatatcgcg
     2341 cataacctgg ctctgcttgg tcgggatgtg catttaatat cagctattgg caacgatttt
     2401 tacggcgaga cattactgga agaaacgcgc cgcgcgggcg tgaacgtttc aaactgcatt
     2461 cgtctgcatg gacacagcac tgcaacttat ctcgctatcg ctaataagca ggaagaaact
     2521 atcctggcaa tcaatgacac acatattctg caacagctaa cgcctcaact gctcaatact
     2581 tcacgggact tgattcgcca tgctggtgtg gtgctggctg attgtaatct cacacctgaa
     2641 gccctggagt gggtctttac cattgctgat gaaattccga tgtttgtcga taccgtttcc
     2701 gaatttaaag cgaacaaagt aaaaaactgg tacagccgga tccacactct gaaaccaaca
     2761 caaaatgaac tggaaatttt gtgggggcaa ccgattaaag acgacaacga tcgcatcaga
     2821 gctgtaaatt cgctgcatca acaaggtgtt aaacgcattt ttgtttatct gaaagatgaa
     2881 tctgtatttt gcagcgataa ggatggagaa caatttttgc tgaccgcgcc agcgcatacg
     2941 acagtagaca gttttggtgc tgacgatggt tttatggcgg gcctggtata tagctttctt
     3001 gaaggataca gtttccgcga cagcgcccgt tttgcggtag cctgcgcggc aatttcgcgc
     3061 gccagcggca gcttaaacaa ccctaccctg tctgccgata acgcgctttc attagtgcca
     3121 atggtgtaac aatgttgccg gattcgacgc ttgaccgcat cttatccggc tcataaattg
     3181 cgcactacgc taaaccaata aagaatcctg caatagtagc actcatcagg ttagaaagtg
     3241 tcgccgccgc cagcgcgcgt aaaccaagtt gggcgatttc cggcgcacgg tgtggcgcaa
     3301 ccgcagaaaa cgcccccacc accaccccga tagaaccaaa gttagcgaaa ccgcacaacg
     3361 cgaaagaaat aatcgccacg gttttagcat ccagagtgcc acccgtttgc agatatggcg
     3421 agaaattgag ataagcgaca aattcattga tcgccagctt ctgcccaatc aaactcccgg
     3481 caagatttgc atcactccag tcaaccccca tcacccacgc caatggggcc aacaggtaac
     3541 ctaaaatcga ctccagcgaa gcatgttcaa aaccaaacca gccgccaaca ccgccgataa
     3601 taccgttaat caacgcaatt attgcaacaa atgccataac aactgtcgct acaccggcgg
     3661 cgattttcag cccggtcatt gcccctgtcg cagcggcttc aataatgctt tttggcggtg
     3721 tttcggtgaa agagaggtta ttaaaagaaa cctgtgaaga ttccgtagcc gggcttaaca
     3781 ggcgggcaaa caagatcccg ccagggatcg ccattaacga tgccgccagc aaatattcca
     3841 caggtacgcc cagtgcggcg taaccaatca ttgtcgaacc agcaatcgag gccatgccac
     3901 tacaaatcgc tgtaaataat tcattgcgat tcagacgatc gataaagggt ttgacgatcg
     3961 ccggaatttc gttttgcccg aggaaaatgg tggtgaccgc gacgaatgac tcgatcttgc
     4021 tgatatttaa agctttctgg aagataccgc cgagaatgcg aattaagatc cccatcacac
     4081 cgatgtagta gagaatactc accagtgcag tgacgaagat aattgccggt aataccctga
     4141 aaccaaagat aaatcctgcg ccatcaaata aggtatccat tttcggtccg accagagaac
     4201 cgaagataaa tgcgctacct gcgtcgctgt acgccatcac tttgtggacg ccaaaagcga
     4261 ctttttctgc gacccaacgc cctggcggta accaaagcat aatcccgcca atcacgacct
     4321 gtaacactaa cgccgcgcca acggtacgca ggctgatctt cttcttgttt actgacagta
     4381 aaaacgcaat tgccagcagt accaccattc ccagaacact tctcatgaca tccatagtga
     4441 ttatctcttc atgtcatttt gggcgacggc tttctgccca tcagaaatag ccgggcctgc
     4501 atcttgcgat actgtcccgg cttgttatca acacttaatg ggtttttatg tagccgcgta
     4561 cgcactcttc gactaatccc cagaaccagt cggtatcaat agtgatgccg actttagtat
     4621 tggcgggctt gccaagaacg cccagctcgt cgcagacggt acgcccatag caagggccac
     4681 tgttgacgtc cacttcgacg tacatctcct gggttttaat gccatcaggg ttaatcagat
     4741 aaccgatgca ggtggcgtcg tgcaccgggc cgccagccag gccgtagttt tcgaactgcg
     4801 ttttgagagt gaagttcatg atgtcgctga acatctctcc ggcagggccg cctgcccttt
     4861 ccattcgtgc aattacgtcc ggggtgcaaa cggtctggtt ggtaagatcg aggcccatca
     4921 tcactaatgg aacgccggag gtgaacacca cgcgtgcggc ttccgggtcg gcaaagatat
     4981 tgaactcggc agatggggtg aagttgcctg taccgtaagc gccgcccatt agcacaattt
     5041 cacggatttt gggcaggatt gcgggttgca tacgcattgc caccgcgata tttgaaagcg
     5101 gaccaaccgg caccagagtg atatcgccat cgcttgccat aagggtatca atgatgtatt
     5161 tcaccgcgtg ggtactttct gcctggcggg tcagcggctc gaataccggg ccatccagtc
     5221 cggtttcacc gtgaatatta tcggcaacga tttgttgacg cataatgggt tgcggcatcc
     5281 ccgcataaac cggaacatta atctccagtt tctggcaaac attcaggcca ttaattaatg
     5341 ttttatcaag cgtctgatta cccgcaacaa tggtgatacc taataaatca atcgccggat
     5401 gtttcgccgc catcattata gcaatagcat catcatgacc cggatcacaa tccagaataa
     5461 tttttctctt ttccattgtt tatttcctct gtttccagtt gcgttatttt ttctacagca
     5521 acgaaatttc gatcgggccg atgattgaat cttaacaaca gcgtacgaat gctaaatatg
     5581 agaaatctca tagcggataa acatcgtgaa agaaatccac aataatgatc ttaagcagca
     5641 attgatgagt gaatccgcgt ttaaggattg ctttttaacg gatgtttcag ccgatacgcg
     5701 gctgtttcat tttttagcgc gtgactacat tgtgcaggaa gggcaacagc cgtcctggct
     5761 gttttacctg acgcgaggcc gcgccaggct ttacgccacg ctagctaatg gtcgcgtgtc
     5821 gctgatcgat ttctttgccg ccccctgttt tattggcgag attgagttaa tcgacaaaga
     5881 ccatgaaccg cgtgcggtgc aggctattga agagtgttgg tgccttgcgc tccctatgaa
     5941 acattaccgc ccgctgttat taaacgacac gctattttta cgaaaactct gcgtcacctt
     6001 aagtcataaa aattatcgta atattgtttc tttaactcag aatcaatcat ttccgttagt
     6061 gaatcgcctg gcagcattta tattactctc gcaggaaggt gatctttatc acgaaaagca
     6121 tacgcaagcg gcagagtatt taggcgtttc ttatcgacat cttttatatg ttctcgcgca
     6181 attcattcac gacggtttat taattaaaag caagaaaggg tatctaatta aaaacagaaa
     6241 gcagttgtca ggactggcgc tggagatgga cccggagaat aaattctccg ggatgatgca
     6301 gtaaaaatta ttggcaatag cacgattgcc ggatgcaacg cttaataagg cgtacatccg
     6361 gcaatgaaac tcaagctaaa ccaataaaga acccggcaat ggtcgcgctc atcaggttgg
     6421 aaagcgtcgc cgccgccagc gcccgtaaac caagctgggc gatttccggc gcgcgttttg
     6481 gcgaaatagc cgaaaacgct ccaacgacaa caccgataga accaaagttt gcaaacccac
     6541 agagggcgaa cgagataatg gcaatggtct tgacatccag cgtgccgctc gtttgcagat
     6601 agggcgagaa attgagataa gcaacaaact cattaatcgc cagtttctgc ccaatcaaac
     6661 ttccggcaag atttgcatca ctccagtcca cccccataat ccatgccaac ggggccagca
     6721 catagccgaa aatgctttcc agagagacat tggcaaaacc aaaccagccg ccaactccac
     6781 caataatgcc gttgatcagc gcaatgatcg ctacaaatgc cattaccacc gtcgccacac
     6841 cggcggcgat tttcagcccg gtcattgcac cgttcgccgc agcttcaata atgctttttg
     6901 gcggtgtttc gctaaacgac agattttcaa acgtaacttg cgaaggttcc gtcgccggac
     6961 taagaatacg tgcaaacagg ataccgccag gaatagccat cagcgaagcg gctaacaaat
     7021 agtcaatcgg cacgcccatt ccggcatagc caatcatcat cgaacccgca atagacgcca
     7081 tgccgctaca aatagccgta aatagctcgt tgcgattcat gcgatcaata aaaggtttaa
     7141 cgatcgccgg gatctcattt tgcccgagga aaatagtggt gactgcgaca aaagattcga
     7201 ttttgctgat gttgagggct ttctgaaaaa tactgccaag aatacgaatc agcagcccca
     7261 ttacgccaat gtagtacagc aagctgataa gcgcggtaac gaaaataatc gccgggagta
     7321 cgcgaaaggc gaagataaag cccgcaccgt caaacagaac atccattttg ggaccgacca
     7381 gcgaaccaaa aataaaggca cttcctgcgt cactgtagga catcacttta tggacgccta
     7441 atgccgcctg ctctactgcc cattttcccg ggggaaaata gagcatgatg ccaccaatgg
     7501 caatttgcag cagcagcgca gcgccaaccg tgcgcaagct gatgctcttt ttatttaccg
     7561 acaacacaaa ggcaattgcc agcaatacca ccatccccgc aacacttctc attatatcca
     7621 taatgatttt cccttcatgc cagtaaaccc ggcgtcagcg ccaggttttg gtatgtttga
     7681 tgagtacggg cgacggcttt ctgcccgcca gaaattaacc cacgagacgc tgatattctt
     7741 tggcaatttc gctcgccaga atggcgttat taaagaccag ttgaatattg gatttcaggc
     7801 tatcaccgcc ggttagctcc gcaacacgcg ccagcaggaa cggtgtactc tctttcccaa
     7861 taacaccctg ttcttcggct tcagcaacgg cttgatcaat tgctgcattg atggtttctt
     7921 ctggcatcgc aaactgttcc gggatcgggt tcgctaccac caggccgccg ttcaaaccgg
     7981 tttgccactt cactgccatc gcgcgagcaa tttcgcgggc actgtcaaga cgaatgctga
     8041 cttcaaatgg gctggttcgg cagaaaaacg caggcagcgc ttttgtctga taaccaatta
     8101 acggtacacc gaaggtttct aaatattctg ttgttaatcc taaatcgaga atagattttg
     8161 cccccgcaca aacaacagtg acattggtat ttgccagttc ctgtaaatcg gcagaaatat
     8221 cgaaggtatg ttccgctccg cgatgcacac caccaatacc tccggttgca aaaactttaa
     8281 tcccagccag tgctgcaata atcatggttg atgcaacggt agtcgcgcca tttttccctg
     8341 cagcaacgac aaaaggtaaa tcacgacgac taactttcgt cacgttatgt ccttcacggc
     8401 ccagcaattc gatttcttct ttacttaagc ccactttcat cacaccgcca ataatggcga
     8461 tagtggctgg tactgcgccc tgtttacgaa ttgtttcttc tacttcaatt gcggtttggg
     8521 cattttgtgg gaatggcatt ccgtgagaaa ttatggtcga ttccagcgca acaaccggtt
     8581 ttttattttt taaagcatcc tgtacttcag cggatatttg taataattca ggggacagag
     8641 ttaattcaga cattctgtgt tctccactaa cgatctaaca ttggcaattg ataattcagg
     8701 gttgttggta tattcacagg caagtgccat tgacgaacat ccctgcgcaa atcgaacaga
     8761 atcgataaac ggcataccgt ctacccaaca ggaggccagc cccgccatca tggcgtcgcc
     8821 tgccccagta acattgatga cattcgtttt aataggggcc gaccacccac tttcaccgtt
     8881 gatatcactg tagtaaacgc catcccctcc catgctcagc accaagcggt tcaggccgtg
     8941 ttgatgaaac caggcggcga cctttgcgac atcttcacgt ccggaaagtg caattccgct
     9001 caacgtttcg gcttcaagac ggttaggttt taacgtatgg attttgctca gatgatcgcg
     9061 gattttgacg catttccatg ccgataccgg atcaataaat acaggcgttt ccccgctatt
     9121 ttccagaacc cagaccagcg cctcttcact aaggttgcaa tccgcgacaa taacctttgc
     9181 accgcgaata aactcctgat gctgggcaag aaattctgca gaaatacagt cactaatact
     9241 catatcattt atcgcgacaa gcatttctcc tgtattatcg agcagtgata aatagctgga
     9301 tgtattctca cccggaacaa ttaagcattt atcgacataa acaccggatt gattagtttg
     9361 cgccagtaat gattgcccat aaaaatcacc acctactgca cttaaaagcc aggcattttt
     9421 ccccagtaac gccagattat gagcgatatt ccttcccacg ccacctgggg taaatttaat
     9481 ttttccggga ttcgaatccg cataattcaa tggatgatgt gaatacccag ccacatcaat
     9541 attcgccgaa ccaataatta ctatgtagtc cttttcgcgc atagcacgcc ccctggcaat
     9601 aaattgccaa cgaagaaatt gggtaaaata gccattacag tcaataagac tattagagca
     9661 cgtgttcaga tttaaacaca tgttcataat aagcatattt aacgtaatgt aaatcgatca
     9721 gaaaaaacga aatcggccag atatgaaggc ggtcacaaag tttgtctatt tatgcagcaa
     9781 caaaagagaa agttgtgatg tgtaacacac aatatggcag gcaagaaaaa gggcagagat
     9841 gtttctgccc tccagattgt tgacaacgcg cgctttgttc atcctggatg tggcgtaaac
     9901 gccttatccg acctacaaaa gcgcaaaaaa ttcaatatat tgcagggttc atgtaggtct
     9961 gataagcata gcgcatcagg caattttgcg tttgtcatca tcggggcaga gatgtctcat
    10021 ccccgataat gaagataaat tatgcgtttt tttctactat ttgtgtttcc gggcgtttca
    10081 ggaaggcata ggccaaaccc gccaccagcg taccggcaat aattgctacc aggtaaccca
    10141 ataccggcgt aatagcgcca gggatcagca gaacaaacag accaccgtgt ggtgccatca
    10201 gtttcgcacc aatcgccatt gagattgcgc cagtcagcgc cccaccaacg atacagcacg
    10261 gtagcacacg catcggatca cgagcagcaa acggaattgc accttccgaa ataaagcaca
    10321 gaccaagtac cagagcggct ttgccacctt cctgctgcgc tttgtcgaat ttgcgacgcg
    10381 ccaccattgt tgccagaccc attgccagcg gtggcaccat acctgccgcc ataatcgccg
    10441 ccatcgggcc ataggtttga gtactcagca gacccacacc gaatgcgtac gctgctttgt
    10501 ttaccggacc gcccatgtca gtacacatca tgccaccgag gatcgccccc agcagaaccg
    10561 cattcgcagt ccccatggtc tgcagccagt gagtcaaccc atcgagaatg ccagcaaccg
    10621 gtttaccgat taggtagatc atcgccagac cgaccaccag actggaaatt agcggaatga
    10681 tcaggatcgg tttcagcgcc tccatactct gtggcagttt caattgcgta ctgattaact
    10741 tcgcaatgta accagccagg aagccagcaa ttataccgcc aataaagcca gaaccggtgc
    10801 tgaccgccag cataccgcca atcagacccg gtgtgaggcc cggacgatcg gcaatggaga
    10861 aggcaatata acctgccagt accggcacca tcagcgcaaa ggctgaacca ccaccaatct
    10921 gcatcagcgc cgctgccaac gtacccggct ctttaaacgc ttcgatacca aaagcaaaag
    10981 aaagcgcgat acacagacca cctgcaacaa ccatcggcag catgtaagag acgcccgtca
    11041 gcaagtgacg gtaagcgcct gcactctctt tcttaccttc agtggtcgct gtttgagctt
    11101 tgcccgctgg ttcatacggc gttgcttcag caaccgcttt atccagttcc tgcgcagttt
    11161 tcttcagcgc cagaccggta gaggtgcgat acatcggttt accagcaaat ttcgccagat
    11221 ccacttcgat atctgccgcc acaatcacca gatccgctgc tgcgacttct tcaggggtga
    11281 ttgcattgcc cgcgccaaca gaaccacggg tttcaacttt cacccaccag ccacgttttt
    11341 tcgcttcggt ttcaatggct tcagccgcca taaaggtgtg tgctacgcca gtcgggcaag
    11401 cagtcaccgc aactacgcgt ttcggaccgc tggcagcaac cggtgctgtc gcagcgaccg
    11461 gcgcagtgta aggtttcgca tggcctttgg cttcactcag gaacagctca gggtgcgcaa
    11521 ctgcccggga aatatcaccc agccagacat ttttaccgtt cagcgcgctg tcattcggga
    11581 tggaatcacc gagaacaatc gccatttctg cgtcgttcgg attgtcgatg atttccagtt
    11641 ttgcttttcg cgccgccgcg cccagcaggg tcttcgccat ataggcgcgt gcctgaccga
    11701 gattagcgtc aataatcagc agcgttttca ttatgcctct cctgctgtca gttaaaaggt
    11761 tgtaagtcga cgcgcgccat cattgcggcc aactgcggac gatcggtaat acccacattg
    11821 ctttgactta ccgccagggc tgcaacagct gtcgccagac gcagtgtgtg ttcactggat
    11881 tcacgcatca gcaagccata aatcaggcca ccaaccatag aatcccctgc gccaacggtg
    11941 cttacgacat cgactgacgg tggtttggcg atccattcgc cggaggcatt aacccaaagc
    12001 gcgccttcgg cacccagtga aataacaaca tgcgcaatac cttgttcacg tagcgcatgt
    12061 gcagcttcaa tcacatcttt catttcaggc agtttacggc ctgcccagat ttccaactcg
    12121 cggcggttag gtttcaccag ccacggtgcc gctttcaaac ctgctactaa cgcttcacgg
    12181 ctactatcaa agataatgca aggacactga ctacgcaggc gagtcatcca gtcggtgaac
    12241 gcttccgggc tgacgcctga cggtaagctt ccgctgacac agaccatatc gaactgaccg
    12301 agccagctca gagaatcagt cacaaagcgt tcccagtcgg cgagggtgac ttcaaaaccc
    12361 gagaagttga agtcggtcac ttcgccgtct ttttccgtca gcttaacgtt aattcgggtg
    12421 cgcccctgta caacctggaa acggttggca atgcccagct cgctgaacag ttgctgaaaa
    12481 ccatcctgat tgtctttacc caggaagccg ccaacggtga catcaattcc caggtctttt
    12541 aatactttgg ccacgttgat gcctttaccc gccgcatgca gaccggtggt tttcaccagg
    12601 ttcacttcgc cgcgttcaat ttccgggcag aaaccaacaa ggtcataagc cggattaagg
    12661 gtgatcgtag caacacgtct gctcattatg cgccctcccc aagaccagca gcgatagcgt
    12721 cgccaatggc tttcagcgcc tgttcagcat ctgcaccctg ggcggtaaag cgtaggcgat
    12781 gacctttctt aacgccaagt gccacaactt tcatcagact acgtccgttt gccggtttgc
    12841 cggtgccatc aaggtttgtc acggtaatat cactgttaaa ttgtttaatg gtattgacca
    12901 gcatggtacc tggacgagca tgcaggccgt gttcattgcg caccacaaac tccgcgctta
    12961 acacgtcgtc ggtcggcgca tcatcgctgg tcagcagcgc cagcaacgtt gccgcatccg
    13021 ctttcagcaa gcggtcagct ttattgtcga gcaataaatc agcgagacgc ttaagaaccg
    13081 cgatgggctg atcgtcattc atcgccacac tcaccagcat ggctgccgtt tcgccgtcca
    13141 catcaaaagc atttgccgca cggcttaccg caatcgcgct acgcagattg ccttcggcgc
    13201 tatcgctcag ccagataccc tgtccgagat tcagcggttg ttcattgatg gctttggtga
    13261 cgaaagtggc gtcaactgcc cccacctctt tcagacgcgc agcgttcagc gcctgaagag
    13321 tcagcagatc gctggcgacg atatccagcg tcagcatttc gttgtcgagc ttcagctgct
    13381 cactctgctt ttcgcccatc agtaatgcgc gaagttcttc tgctgttgtt gctgacttca
    13441 gttgttcagc aacggaatca tcgctcagta cgtgggtcag ctggcgtagc aggcccagat
    13501 gttcatccga gctggcagca ataccgattg ccacgtacgc tacctgaccg tcaccccagg
    13561 tgacgccttc cgggaactga aatacctgaa cgccggtttt cagcacctga tcgcgggtgt
    13621 cggtagtgcc gtgtggaata gcaataccat tgccgaggaa cgttgaggtt tgctgttcgc
    13681 gcgccagcat gccattgacg tagccttctg ctacattacc ggcctgcacc agcgccgcag
    13741 cgacctggcg aatcgcctct tctttgtctc cggccttttc gcccggatgg atgtcctgta
    13801 cggataactg gaacatagtt ctcctctctt gctgaattga aacgattcag cctctatgag
    13861 aaaaaaagcg ccaacctggc ttagggttaa agacaagatc gcgctgaaac gtttcaagaa
    13921 agcataatac ttctgtttca gcacgcaagg aaagccgtaa agttatgagc aaaagtttga
    13981 tgtgctgcac aattttttgt catttttcct aattgctgat gggaaaatcc gttatcagca
    14041 atttcatttc agcagcgtca gcagccaatc agcaacagtg aaatggcatt ttgattattc
    14101 gtggcgcttt tgatttcgct gtctgtttat tttctgacaa gcagcgtaaa ctccgcgtct
    14161 tcctcttcca gtgatcgacc tgcatgcata actcccccgc agtctccagc gcgaaatcgt
    14221 ttgacctgac ctcgacggcg tttttaatcg ttgcctttct caccggtatt gcgggtgctc
    14281 tgcaaacccc gacactcagt attttcctta ccgatgaagt acatgcccgt ccggcgatgg
    14341 tgggattctt ctttaccggc agcgctgtca ttgggattct ggtaagtcag tttctcgctg
    14401 ggcgctctga taagcgcggc gatcgcaaat cgctgattgt cttttgctgc ctgttaggcg
    14461 tgctggcctg cacccttttt gcctggaatc gcaactactt tgttttgcta ttcgttggcg
    14521 tctttcttag cagctttggc tcgaccgcta acccgcaaat gtttgccctt gcccgtgaac
    14581 atgccgacaa aaccggacgt gaggcggtga tgttcagctc ttttttacgc gctcaggttt
    14641 cactggcatg ggtcattggc ccaccgctgg cttatgcgtt agcgatgggt ttcagcttta
    14701 cggtaatgta tctgagcgca gcggtagcgt ttattgtttg cggcgtgatg gtgtggctgt
    14761 ttttaccgtc gatgcaaaaa gagcttccgc tggcgaccgg cacggttgaa gcgccgcgcc
    14821 gtaaccgtcg cgatacgcta ctgctgtttg tcatttgtac attgatgtgg ggctcgaaca
    14881 gcctgtacat catcaacatg ccgctattta ttatcaacga actgcatctt cccgagaaac
    14941 tggccgatgt gatgatgggg accgccgccg ggctggaaat cccgacgatg ttgattgccg
    15001 gatatttcgc caaacgtctg ggtaagcgtt tcttaatgcg cgttgctgcc gtgggtggcg
    15061 tctgttttta cgcaggaatg ctgatggcgc attcacctgt cattctgttg ggcttgcagc
    15121 tgctaaatgc tatttttatt ggcattctgg gcggcatcgg gatgctctat tttcaggatc
    15181 tgatgcccgg tcaggcgggt tcagccacca cgctctatac caacacttcg cgcgtgggct
    15241 ggatcatcgc aggatcagtg gcgggcatcg tcgccgaaat ctggaattat cacgctgtgt
    15301 tctggtttgc gatggtgatg attatcgcca ctctgttttg cttactgcgg attaaagatg
    15361 tttaaggcgc ggtcagcatt tccagatcga taagccaggt catggcctgc tgacgcgagt
    15421 taccgcacat ttcagcgctg gcttgtaagc ctgcacagac tttcggtcgc agtggcgagg
    15481 tgaaaatttt acagcgttgc tgttcatcaa gctgaatgca gggcgtattg gcgggcttgc
    15541 catctggcat accgggaata gggctggaaa ttgaaggggc agtgcaacac gccccgcaac
    15601 ccggacggca ttccatacgt tgttctctta aggttaacca ccggattgat gagcgcgcac
    15661 agtaccacct tttttgcacc agcaaaagtg cgaataccac ttgcccgaaa ggcccgtcgc
    15721 gagtactttg tcgcgatatt tttgacattt tcgactacag gaatttttcg atgccaagag
    15781 cgaacgaaat taaaaaaggt atggtactga attacaacgg caaactgctg ctggttaagg
    15841 atattgatat tcagtcgccc actgcccgcg gtgccgctac gctgtacaaa atgcgttttt
    15901 ctgatgtccg taccgggctg aaagtagaag agcgtttcaa aggtgatgat atcgttgaca
    15961 ccgtgacgct gacccgccgt tacgttgact tctcctatgt cgatggcaac gaatatgtct
    16021 ttatggataa agaagactat accccgtata ccttcaccaa agatcagatt gaagaagagt
    16081 tgctgtttat gccagagggc ggcatgccgg acatgcaggt gctgacctgg gacggtcaac
    16141 tgctggcgct tgagctgccg cagaccgttg atctggaaat cgttgaaacc gcaccaggca
    16201 tcaaaggggc atctgccagc gcccgtaaca aaccggcgac attgagcact ggtctggtga
    16261 ttcaggtacc agaatactta agcccgggcg aaaaaattcg tatccatatc gaagaacgcc
    16321 gttatatggg gcgtgctgac taacttcagc cgcatgcaga aaagggatag ctcaggctgt
    16381 ccctttttta atttattata ccaatcttct attttgcgct tcctgaacac cttacgccct
    16441 caattttcac tcgttgattg atcgccctca cacttcatcg cattaacaat ccagaccaat
    16501 ttcaattgct gtcatataac tttacactgt cattgttaat ttatcgttac taagacgtga
    16561 ctcctatgaa gacaattgcc tccgttacgc tcccgcatca tgtacacgct ccacgctacg
    16621 atcgccagca gttgcaatca cgtatcgttc attttggctt tggcgccttt caccgcgctc
    16681 atcaggcgtt actgaccgat cgtgtgctga atgcccaggg cggcgactgg gggatctgtg
    16741 aaatcagctt gttcagcggt gatcaactga tgagccagct ccgcgcacag aaccatttat
    16801 ataccgtgct ggagaaaggt gcggacggca atcaggtgat aattgtcggt gccgttcacg
    16861 aatgccttaa tgcaaaactg gattccttag cggcaattat tgagaaattt tgcgagccac
    16921 aggtggcaat tgtttccctg acgattaccg aaaaaggcta ttgtattgac ccggccaccg
    16981 gtgcactcga caccagtaat ccgcggatta ttcacgatct acaaacccct gaagaacctc
    17041 actccgcacc gggtattctc gtcgaagcac tgaaacgccg ccgtgagcgc ggccttacac
    17101 cgtttaccgt gctctcctgc gacaatattc ccgacaatgg ccatgtggtg aaaaacgcgg
    17161 tgctgggaat ggcagaaaaa tgttcgccag aactcgccgg gtggataaaa gaacacgtca
    17221 gttttccggg aaccatggtc gaccgcattg ttccggctgc aaccgacgaa tcactggcag
    17281 aaatcagcca gcatctgggg gtaaatgatc cctgcgcgat tagctgcgaa ccgtttatcc
    17341 agtgggtggt tgaagataac ttcatcgctg ggcgtcctgc ctgggaagtc gcaggtgtac
    17401 aaatggtgaa tgatgtcctg ccatgggaag agatgaaact gcgtatgctt aatggcagcc
    17461 actcttttct cgcttatctg ggttacctct caggattcgt ccatatcagt gattgcatgc
    17521 aggatcgcgt atttcgccat gctgccagaa cattaatgct ggatgagcaa gcgccgacac
    17581 tgcaaattaa agatgtcgat ttaacacaat atgcggataa gttaattgca cgttttgcta
    17641 atccgtcgct gaaacataag acctggcaaa tcgcgatgga tggcagccag aaattaccgc
    17701 aacgtatgct ggcaggtatt cgcatacatc tggggcgtga aacggactgg tcgttgctgg
    17761 cattaggcgt tgctggctgg atgcgttacg tcagcggcgt tgatgatgcc ggaaatgcca
    17821 ttgatgttcg cgatccgctt agcgataaaa ttcgcgaact tgttgcgggc agcagcagtg
    17881 aacaacgcgt aaccgccctg ctttccctgc gtgaagtttt cggtgatgat ctgccagata
    17941 acccgcattt tgtgcaggcc atcgaacaag cctggcaaca aatcgtacaa ttcggcgcac
    18001 atcaggcgct attaaacacc ctcaaaattt aacgatttct gcggttaaag cggatgaagc
    18061 tcaccttcgt ccgctctccc cttctctttt ctgccttttt tagccaggat taacgctcag
    18121 ttaacttacc agagtattgc ggagccgttg tgaccaggac caacctcatc accggttttc
    18181 tcggcagcgg gaaaaccacg tcgattcttc atctgttagc ccataaagat cccaacgaaa
    18241 aatgggcggt actggttaat gaatttgggg aagtcggaat tgatggtgct ttgctcgccg
    18301 atagcggcgc attgctgaaa gagatccccg gcggctgcat gtgctgcgtt aatggtttac
    18361 ccatgcaggt agggttgaat accttactgc gtcagggaaa accagaccgc ttgttgatag
    18421 agccgaccgg gctgggccat ccgaaacaga tcctcgatct gttaaccgca ccagtctatg
    18481 aactgtggat agatctgcgc gccaccttgt gcattctcga tccacgcctg ctgctggacg
    18541 aaaaaagcgc cagcaatgaa aacttccgtg accagctggc tgccgcagac atcattgtcg
    18601 ccaataaatc cgaccgtgcg acgcccgaaa gtgagcaagc gctacagcgt tggtggcagc
    18661 aaaatggtgg cgatcgacaa ttaattcaca gtgagcatgg gaaagttgac ggtcatcttc
    18721 tggatttgcc gcgtcgcaat ttagccgagt tgcccgccag cgccgcgcat tctcatcagc
    18781 atgtcgtgaa aaaagggtta gcagcgttaa gcctgccaga gcatcaacgc tggcgtcgca
    18841 gtctgaacag cgggcaagga tatcaggcct gcggctggat attcgacgct gatacggtat
    18901 tcgacaccat tggcattctg gaatgggcgc gacttgcacc ggtggaacgc gtcaaaggcg
    18961 tgctgcgtat tcccgaaggg ctggtacgaa tcaaccgtca gggcgatgac ctgcacattg
    19021 aaacgcaaaa cgttgcgcca ccggacagcc gtattgagct gatttccagc agcgaagctg
    19081 actggaatgc tttgcagagc gcgctgttga agcttcgttt agcgactacc gcgtaaggtt
    19141 gcctgcgttt ttcagtaaga taattagaga aaatatgatt aaaaatttgc cgcaaatagt
    19201 gttgttgaat attgtcggcc tcgcgctgtt tctttcctgg tatattcccg ttaatcatgg
    19261 attctggttg ccgattgatg cggatatttt ttatttcttt aatcagaaac tggtcgaaag
    19321 taaggccttt ttgtggctgg ttgcattgac caacaatcgc gctttcgacg gttgttctct
    19381 gctggcgatg ggaatgttga tgctgagttt ctggctgaaa gaaaacgccc ctggcagacg
    19441 acgtatcgtg attatgggtc tggtcatgct attaactgca gtggtattaa accagctggg
    19501 tcaggcatta attcctgtaa aacgggccag cccaacattg acttttaccg atattaaccg
    19561 cgtcagcgaa ctgctctctg ttcccacgaa agatgcctca cgagatagct ttcccggcga
    19621 tcacggcatg atgctgctta ttttttcggc attcatgtgg cgttatttcg gcaaagttgc
    19681 aggccttatc gcccttatta tttttgtggt ttttgcattt cccagagtaa tgattggcgc
    19741 acactggttt actgacatca ttgtcggttc gatgaccgtg atattgatcg gtttgccctg
    19801 ggtgttgctg acgccattaa gtgatcgatt aatcaccttt tttgacaaat cactaccggg
    19861 aaaaaacaaa catttccaaa acaaataact cacagtaatt aacatcatca ggcttatttt
    19921 tatagtgagg ataatcctga tgatgcgcac cgtgctttca tccatcgcgc gcaaaaatca
    19981 ttctctaagt aaatgaatgg attgcatgcg tttcactcaa tcgtacttta attgaccaat
    20041 cccgcttatt aactttctgt atcacttttt cttataaaaa atcatgtaaa accgctcgcc
    20101 aagaccgcac caatcgggta atctcgaact cgttttgcct cggcggtaga ttatcctcac
    20161 agcatataat tttgtgcgtt agtccacaga tttggcctta aggaattgtt tcaacatgcc
    20221 caggtaatta gtctcgtgcc gcttggcatt tttttataac gatatttgtc gttaaggact
    20281 tcaagggaaa acaaacaaca tggtcaaatc tcaaccgatt ttgagatata tcttgcgcgg
    20341 gatccccgcg attgcagtag cggttctgct ttctgcatgt agtgcaaata acaccgcaaa
    20401 gaatatgcat cctgagacac gtgcagtggg tagtgaaaca tcatcactgc aagcttctca
    20461 ggatgaattt gaaaacctgg ttcgtaatgt cgacgtaaaa tcgcgaatta tggatcagta
    20521 tgctgactgg aaaggcgtac gttatcgtct gggcggcagc actaaaaaag gtatcgattg
    20581 ttctggtttt gtacaacgta cattccgtga gcaatttggc ttagaacttc cgcgttcgac
    20641 ttacgaacag caggaaatgg gtaaatctgt ttcccgcagt aatttgcgta cgggtgattt
    20701 agttctgttc cgtgccggtt caacgggacg ccatgtcggt atttatatcg gcaacaatca
    20761 gtttgtccat gcttccacca gcagtggtgt tattatttcc agcatgaatg aaccgtactg
    20821 gaagaagcgt tacaacgaag cacgccgggt tctcagccgc agctaataaa ccgtttggat
    20881 gcaatccctt ggctatcctg acgagttaac tgaaagcact gcttaggcag tgcttttttg
    20941 ttttcattca tcagagaaaa tgatgtttcc gcgtcttgat ccaggctata gtccggtcat
    21001 tgttatcttt taaatgttgt cgtaatttca ggaaattaac ggaatcatgt tcatacgcgc
    21061 tcccaattct ggacgtaagc tcctgcttac ctgcattgta gcaggcgtga tgattgcgat
    21121 actggttagc tgccttcagt ttttagtggc ctggcataag cacgaagtca aatacgacac
    21181 actgattatc gacgtacaaa agtatctcga tacctatttt gccgacctga aatccactac
    21241 tgaccggctt cagccgctga ccttagatac ctgccagcag gctaaccccg aactgaccgc
    21301 tcgcgcggcg tttagcatga atgtccgtac gtttgtgctg gtgaaagata aaaaaacatt
    21361 ctgttcatct gcgactggtg agatggacat tccactcaat gaattgattc cggcgctcga
    21421 cattaataaa aacgtcgata tggcgatctt acccggcacg ccgatggtgc cgaacaaacc
    21481 cgcaatcgtc atctggtatc gcaacccttt gctgaaaaat agcggtgtgt ttgccgctct
    21541 gaatctcaac ctgacgcctt ctctctttta tagttcacgg caggaagatt acgatggcgt
    21601 cgccctcatt attggcaata ctgcgctatc taccttttct tcacgtttga tgaacgttaa
    21661 tgaattaacc gacatgccgg tccgtgaaac taaaattgcg ggcattcctc tgaccgttcg
    21721 gctttatgcg gatgactgga catggaacga tgtgtggtac gcatttttac tgggcggcat
    21781 gagtggaact gtcgttggcc tgctctgcta ttacctgatg agcgtacgta tgcgccccgg
    21841 cagagaaatc atgaccgcca tcaagcgcga acaattttac gtggcgtatc aaccggtggt
    21901 ggatacacaa gctttgcgag taacgggcct ggaagtactg ctacgctggc ggcatcctgt
    21961 cgcgggagaa attcccccgg atgccttcat taactttgcc gaatcgcaaa agatgattgt
    22021 gccgctgact cagcacctgt ttgagttaat tgcccgcgat gccgcagaat tagaaaaagt
    22081 gctgccagta ggcgtcaaat ttggtattaa cattgcgccg gaccatctgc acagcgaaag
    22141 ctttaaagca gatatccaga aactgctcac ttccctgccc gcacaccatt tccagattgt
    22201 gctggaaatt accgagcgcg atatgctgaa agagcaagaa gccacacaac tcttcgcctg
    22261 gctgcactcg gtcggcgtag aaattgctat tgatgacttc ggcaccgggc acagcgcgct
    22321 tatctatctt gagcgtttta cgctcgatta tctgaaaatt gaccgtggat ttatcaacgc
    22381 catcggtacg gaaacgatca cttcacccgt acttgacgcg gtgctgacgc tggcgaaacg
    22441 cctcaatatg ctgacggttg ctgaaggggt cgaaacgccg gaacaggcgc gatggctaag
    22501 cgaacgcggc gttaatttca tgcaaggcta ctggattagt cgccctttac cgctggacga
    22561 ttttgtccgc tggctgaaga aaccgtatac gccgcagtgg taaggtgtgc ttacgtccct
    22621 tattattcat agtgaaagca tgccggattg cggctaatga tgagtaaaag gaaatccatt
    22681 gcagatgatt gtgcgcatac tgctgctgtt tatcgctctg ttcacctttg gtgtgcaggc
    22741 gcaggctatc aaggaaagct atgcctttgc cgtgctgggc gaaccccggt acgcgtttaa
    22801 tttcaaccat tttgattatg tgaaccccgc cgcgccaaaa ggtgggcaga taacgttgtc
    22861 agccctcggc accttcgata atttcaaccg ctatgcactg cgcggcaacc cgggcgcacg
    22921 caccgagcag ctgtacgaca cgctatttac gacttccgat gacgaaccag gcagttatta
    22981 cccgctgatt gctgaaagcg cacgctatgc tgacgattat tcctgggtgg aggtcgctat
    23041 taatccgcgc gcccgttttc atgatggttc gcccattact gcccgcgatg tagagtttac
    23101 ttttcaaaaa tttatgaccg aaggcgtgcc gcaatttcgt ctggtctaca aaggcaccac
    23161 cgtcaaagcc attgcaccgt taaccgtgcg cattgagtta gctaaaccca gcaaagaaga
    23221 tatgctgagt ctgttttcgc tgccgatatt tccagaaaag tactggaagg atcacaaact
    23281 tagcgacccg ctcgccacgc ctccgcttgc cagtggtccg taccgcgtta cgtcctggaa
    23341 aatggggcaa aatattgtct attcccgcgt gaaagattac tgggcagcaa acttaccggt
    23401 aaaccgtgga cgctggaatt tcgacaccat tcgctacgat tattacctcg atgataatgt
    23461 cgcctttgaa gcgtttaaag caggtgcctt tgatttgcgt atggaaaacg acgctaaaaa
    23521 ctgggccacg cgttataccg gtaaaaattt cgataaaaaa tacatcatca aagatgagca
    23581 aaagaacgaa tcagcccagg atacgcgctg gctggcgttt aatatccaac gtccggtatt
    23641 cagcgatcgc cgggtccggg aagcaatcac tctcgccttt gactttgaat ggatgaacaa
    23701 ggcgttgttt tacaacgcct ggagtcgcac gaacagttat tttcagaata ccgaatacgc
    23761 ggccagaaat taccccgacg ccgcggagct ggtgcttctg gcaccaatga aaaaagatct
    23821 accgccagaa gtcttcacac aaatctacca gccgccggta tctaaaggcg atggctacga
    23881 tcgtgacaac ctgttaaaag ccgacaaact tctcaacgaa acgggctggg tgctgaaggg
    23941 tcagcaacgc gttaatgcca caacgggtca gccgctcagc tttgaattat tgcttcccgc
    24001 aagcagcaat agtcagtggg tattgccgtt ccagcacagc ctgcaacggc tgggtatcaa
    24061 tatggacatt cgcaaggtgg ataactcgca aatcactaac cgcatgcgca gtcgcgacta
    24121 tgacatgatg ccgcgcctat ggcgggcgat gccgtggccc agttccgatt tacagatttc
    24181 ctggtcatcg gaatatatca attccactta taatgccccc ggcgtgcaaa gcccggttat
    24241 cgactcgctg atcaaccaaa ttattgccgc gcagggaaat aaagaaaaat tactgccgtt
    24301 ggggcgagca ctggatcgcg tattaacgtg gaattattac atgctgccaa tgtggtacat
    24361 ggcggaagac cgtctcgcct ggtgggataa attctcccaa cccgctgtac gccctgttta
    24421 cagcctgggt atcgatacct ggtggtatga cgttaataaa gcggccaaac tgccgtcagc
    24481 cagacaacag ggagagtaga tgggcgctta cctgattcgc cgtctgttgc tggtgatccc
    24541 aacgttatgg gctattatca ctatcaactt tttcatcgtg caaattgcgc ctggcggtcc
    24601 ggtcgatcag gccatcgccg ccattgagtt tggtaatgcc ggagtattac ccggcgcagg
    24661 cggtgaaggt gttcgtgcca gccatgcgca aacgggtgtc ggcaatatca gcgacagtaa
    24721 ttaccgtggc ggacgcggat tagacccaga agtgatcgct gagatcactc atcgctacgg
    24781 tttcgataag ccgatccacg aacgttactt caaaatgctc tgggactaca tccgctttga
    24841 ttttggtgat agcctgtttc gcagcgcctc ggtgctgacg ctgattaaag acagtctgcc
    24901 ggtttccatc accctcggat tgtggagcac gctgattatc tatctggtgt cgattccgtt
    24961 aggcattcgc aaagctgttt ataatgggag tcgctttgac gtctggagta gcgcatttat
    25021 catcatcggc tacgccattc cggccttttt gtttgccatc ctgctgattg tcttcttcgc
    25081 gggcggcagc tatttcgacc tgttccctct acgcggcctg gtttccgcta actttgattc
    25141 gctgccgtgg tatcagaaaa tcaccgatta tctgtggcat atcacgctac cggtgctggc
    25201 gacagtgatt ggtggctttg cggcgctgac catgctgacc aaaaactcat tccttgatga
    25261 agtgcgcaag caatacgtgg tgaccgcgcg tgcgaaaggg gtaagtgaga aaaacattct
    25321 ctggaaacat gtgttccgca acgccatgct gctggtgatt gccggttttc cggcgacgtt
    25381 tatcagcatg ttttttaccg gctcgctgct gattgaggtg atgttttcac tcaatggtct
    25441 tggcttactg ggctacgaag cgaccgtctc gcgcgattat cctgtaatgt ttggtacctt
    25501 gtatattttc accctgattg gcctgctgct gaatattgtc agtgatatca gctatacgct
    25561 ggttgatccg cgtatagatt ttgagggacg ttaatgtcgc gactcagccc cgtcaatcag
    25621 gcccgttggg cgcgttttcg tcataaccgt cgcggctact ggtcgttatg gattttcctc
    25681 gttttgtttg gtttgagttt gtgttctgaa cttatcgcta acgataaacc gttgctggtg
    25741 cgttatgacg gcagttggta tttcccgttg ttgaaaaact acagcgaaag cgattttggc
    25801 ggcccgctgg caagtcaggc tgattatcag gacccgtggc tgaaacaacg gctggaaaat
    25861 aacggctggg tactgtgggc accgattcgc tttggtgcta ccagtatcaa ctttgctacc
    25921 gataagccct tcccttctcc accctcccgg caaaactggc tgggaacgga tgccaacggc
    25981 ggcgatgtgc tggcacgcat tctctatggc acgcggatct cggttctgtt tggcctgatg
    26041 ctgactctct gttccagcgt gatgggcgtg ctggcggggg cgctacaagg ctattacggc
    26101 ggtaaagtcg atctctgggg acaacgcttt attgaagtat ggtcggggat gccgacgctg
    26161 tttttgatta ttttactttc cagcgtcgta cagcctaact tctggtggct gctggcaatt
    26221 actgtcttgt ttggctggat gagtctggtc ggcgtggtgc gggcggagtt tttacgtact
    26281 cgtaatttcg actacattcg tgcggcacag gcgcttggcg tcagcgatcg cagtatcatc
    26341 ctgcgtcata tgttgcctaa tgccatggtc gcgaccctca cctttttacc gtttatttta
    26401 tgtagttcga taaccaccct gacctcgctc gatttcctcg gcttcggtct accgctcggt
    26461 tcaccgtcac tcggtgaact gctgttacaa gggaaaaata accttcaggc cccgtggctt
    26521 gggatcaccg ccttcttgtc ggtggcgata ttattgtctt tgctgatctt tattggtgaa
    26581 gccgtccgcg acgcatttga tcctaataag gcggtgtagc atgacgcaaa ctctgttagc
    26641 gattgaaaat ttgtcggtgg gttttcgcca tcagcaaacc gtacgtacag tagtcaatga
    26701 tgtttcacta cagattgagg ctggcgaaac gctggcgctg gtgggtgagt caggttcagg
    26761 caaaagcgtt accgcgctgt caattttacg cctgctccct tccccgccgg ttgaatatct
    26821 ctccggcgat attcgttttc atggcgaatc gctgcttcat gccagcgatc aaacgttgcg
    26881 cggtgtacgc ggtaataaga tcgccatgat ttttcaggaa ccgatggtgt cgttaaatcc
    26941 attgcatacc ctggaaaaac agctttatga agtgctttca ctccaccgcg ggatgcgtcg
    27001 ggaagcggct cgtggcgaaa ttcttaactg ccttgatcgc gtcggtatcc gccaggcggc
    27061 aaaacggctg acagattatc cgcatcagct ctccggcggc gaacgccagc gggtgatgat
    27121 tgcgatggcg ctgttaacgc gaccggaatt attaattgcc gatgaaccga ccaccgcact
    27181 ggacgtctct gtccaggcgc agattttaca gctgttgcgc gaactgcaag gcgagctgaa
    27241 tatgggcatg ctgtttatta ctcataacct cagcattgtc agaaaactgg cccaccgcgt
    27301 ggcggtaatg caaaacggtc gctgtgtcga gcaaaataac gccgctacgc tatttgcctc
    27361 acccactcat ccttacacac aaaagctact caacagtgaa ccgtcaggcg atccggtacc
    27421 gttgccagaa cctgcctcaa cgttgctgga tgttgaacag cttcaggttg ccttccccat
    27481 tcgcaaaggg attttgaagc gcattgtgga tcataatgtg gtggtgaaaa acatcagttt
    27541 tacgctacgg gcgggtgaaa cactgggttt agtgggcgag tctggttccg ggaaaagcac
    27601 gacgggactg gcgctgctgc gactgattac ctctcagggc agcatcgtct ttgacggtca
    27661 gccactgcaa gatttaaatc gccgccagct gttacctact cgtcatcgta ttcaggtggt
    27721 atttcaggat ccaaactcct cgctcaaccc acgactcaac gttttgcaga ttattgagga
    27781 aggcttacgg gttcaccagc cgacgctttc tgccgcacaa cgcgaacaac aagtgatagc
    27841 cgtgatgcat gaagtgggat tagatcctga aacacgccac cgttatccgg cggagttctc
    27901 tggtggtcag cgacaacgta ttgcgattgc cagggcatta attcttaagc cctcactgat
    27961 catacttgat gaaccgacat catcactcga caaaacggta caggcgcaaa tattgacgct
    28021 attgaaatca ttgcaacaaa agcatcaact ggcctatttg tttatcagcc acgatttgca
    28081 cgttgtccgc gcgttatgtc atcaggttat cgtactgcga caaggggaag tagtggaaca
    28141 aggaccgtgc gcgcgcgtgt ttgccgcacc gcagcaggag tatacgcgtc agctactggc
    28201 gttgagctga cgcttaaaaa ggattgtagt ttgaaaaggg ttcggcaatt gccacaccaa
    28261 aatttttcaa tcggcatgtt gcagcaaact cgtcctggcg atttacaaac aggcacggct
    28321 caccttcaca ctccagcacc gaacatggaa cttcgatgtc gcttaacgcc tggcttagtt
    28381 tatacatcac cgaccatgca ttatcaccat ccgggctaag caatttaagc gccaccaggc
    28441 tgttgtcacc gcacggtcca ttttgcggaa tcggttcaac cttcgaacct gcgaaaccag
    28501 cagaccagcg aaagttctgc ggtagacgat ggacgattga gagttgtaat gaagtcactt
    28561 attttccccg gaagcacatt tacttcacaa tttatttaca tcaattttaa cacatcatca
    28621 acaatccgtc tttatacaga tcgtaaagat tgatgcctcg cctgcatggc ctctatggct
    28681 ctgatttaag taatatctgc gggcgtggtc acgtttgcgg ggctatatgt acccgtttct
    28741 gcgatctaac tcaacctttt taactacaat gatgtgactt tttacataaa ttgattttac
    28801 ataaaataaa catatatcgg gggaatgata gatttgtggt tgacatgcat caacaaagga
    28861 agccttttag cttcctcgtt gtgcaataga tcaccgtttt ttcggtcgac tggcgtacag
    28921 acagaagaga atggagctgg ttgcgcagaa tgcaattgac caaatcatcg gccatgcaga
    28981 gttaaaggtc gcaagagaaa gcaatgcgcc aacaattgcc cctatgccaa aacggaaggt
    29041 tcctgccagc gaagatgccg ttcccgccat atggggaaac tcatcaagaa tgaccgccat
    29101 cgcattggat gacaccatag acacgcagcc cacaaacgcc gcaacgccaa ccaccagcga
    29161 ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa taaattgtat
    29221 ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cggacgaagc ggctgttaaa
    29281 gatggtcatc acgaacagaa aaacaatgtt tagcgcaaag taataaccaa agttttccgg
    29341 tgcaacgtgg ttgatttcaa tgtagacaaa cggtccggcg ctcaggaacg agaacattcc
    29401 ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac ggaacagcgc
    29461 cgcaaagtta ccaatagtgg tacgaatgtg aaaaggctgg cgaggatcca gaagatgtaa
    29521 tgccagctca gccacaccag cacccagccg ccaactatcg gtgccatcag cggtgcaatg
    29581 gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact cttctttcgg gtaaatatca
    29641 cgcatcaaag cgttgataac cacgctggcc gcagccgcag ccagcccgtg gaagaaacgc
    29701 atcacaatca gctgatcgat ggtttgtgcc aacgcacacg ccaccgcggc ggcggcaaac
    29761 accagcgtac cgccaagaac caccggctta cgcccgaagc tgtctgccat cggcccgtag
    29821 attaactgcc ccaacgcaaa gcccagaata taagtactca gggtcatctg cgtactgccc
    29881 gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg gcagatacat atcaatcgac
    29941 agcggcatca acatggccag caggccaagg ataaaaacaa tagcaaacga cgaatgctgt
    30001 cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt agacgacgct ggcaatttct
    30061 tcttcagtta acggacgata ttcaccgggg gctaaatcag catccagcgt aatgccgcca
    30121 atacgttcac gatgcagctc aaccacatgg ttacccacgg cggcgaacat gcgtttcacc
    30181 tgatgataac gcccttcgct gatggtcaga cgaacctgcg ttggggtaat cacttccagc
    30241 accgccggct tagtgagatc tttttcgtta tgcagctgca cgcctttagc aaattgttct
    30301 gccgtatcgt cagctacggg tgataccagc gtcaccagat aggtcttctc gcaatgatgg
    30361 cgcggagaag taatgcggtg cgaccactga ccatcatctg tcattagtac cagaccggtg
    30421 gtatcgatat ccagccgccc tgcggcgtgc agtttccacg ctaccggttc atctagaaaa
    30481 tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa cataaccctg aggcttattg
    30541 agcatgaagt aacgtggacc gtgttgctgc gccagcgggt tgccatcgta agcgacatca
    30601 tgttcaggaa gcagtttgaa cgctgcatta cggacgattt cgccatcgac ggtgacacga
    30661 ttgccgcgga tttcacgccc ggcaatagca cggctaacgc cgagttgctg tgcgataaat
    30721 ttatcaagtc gcatgtgtgt gattttgcct gtaaaaaacg gaggtcgggt aatgacccga
    30781 atatctgaac tgttgtctgc ccagtatagc ggtctaataa catccctcaa gggaaaaaga
    30841 tccgtggcat actattagca gaataatcta cctacgcgag accatgattt ttacacttcg
    30901 cccatatcag caagaagccg tggatgccac gctcaaccat tttcgtcgtc ataaaacccc
    30961 tgccgttatc gtgctgccca ccggtgcagg taaaagcctg gtgatagcgg aactggcacg
    31021 gctggctcgt ggtcgcgtac tggtgctggc acacgttaaa gaactggtgg cgcaaaacca
    31081 tgcaaagtat caggcgctgg ggctggaagc cgatattttt gccgccgggc tgaagcgtaa
    31141 agagagccac ggtaaagtgg tatttggcag tgtgcagtcg gtcgcccgta atattgatgc
    31201 ctttcagggt gaattttcgc tgttgattgt cgatgaatgt caccgtattg gtgacgatga
    31261 agagagccag tatcagcaaa tcctcactca cctgacaaaa gtgaatcccc acttacgcct
    31321 gctggggctg actgccacgc cttttcgatt gggcaaaggt tggatctacc agttccatta
    31381 tcacggcatg gtacgcagcg atgagaaagc ccttttccgt gactgcattt atgagctgcc
    31441 cctgcgttat atgattaaac acagctatct gacgccgcca gaacgactgg atatgccagt
    31501 agtgcaatat gatttcagcc gcttgcaggc acagagcaac gggctgttca gcgaagccga
    31561 tctcaaccgt gagctgaaaa aacagcaacg tattaccccg cacatcatca gccagattat
    31621 ggagttcgcc gaaaagcgta aaggagtgat gattttcgcc gcgacggtcg aacacgcaaa
    31681 agagattgtg ggattactgc ctgccgaaga tgcagcactg attactggcg acacccccgg
    31741 cgctgagcgc gatgtgttaa ttgaaaattt taaagcccag cgttttcgct atctggtcaa
    31801 cgtcgcggta ctgaccaccg gatttgacgc cccgcacgtc gatcttatcg ccattctgcg
    31861 ccctaccgaa tcagtgagtc tttaccaaca aattgtcggg cgcggtctgc gtctcgctcc
    31921 gggcaagact gattgtttaa ttcttgatta tgcgggtaat cctcacgatc tctacgcgcc
    31981 ggaagttggt acaccgaaag gcaaaagtga caacgttccg gtacaggttt tctgccctgc
    32041 ctgcggtttt gccaacacct tttgggggaa aacgaccgcc gacgggacat tgattgaaca
    32101 ctttggtcgc cgctgtcagg gatggtttga agatgacgac ggtcatcgcg aacagtgtga
    32161 cttccgtttc cgttttaaaa attgcccgca atgtaacgca gaaaatgata ttgccgctcg
    32221 ccgctgccgc gaatgtgaca cggtactggt tgatccggat gatatgttaa aagcggcgct
    32281 acgactgaaa gatgcgctgg tattacgctg tagcggcatg tctttgcagc atgggcacga
    32341 cgagaaaggc gaatggttga aaatcaccta ttacgatgaa gacggtgcgg atgtgagtga
    32401 gcgtttccgt ctgcaaacac ctgcccagcg taccgccttc gagcagcttt ttatccgccc
    32461 gcatacgcgc acaccgggca tcccgctgcg ctggatcacc gccgccgata tcctcgccca
    32521 gcaagcctta ttgcgacccc cggattttgt cgtcgcccgc atgaaaggcc agtactggca
    32581 ggtgcgtgaa aaagtgttcg attacgaagg tcgttttcgt cgggcgcacg aattacgcgg
    32641 ttaatggtag ttttgattga tgtataaggc gattgagtat agaatctcac ccgcttttgc
    32701 atacgcaaag cagatcactt acctgttgct gggtcgcctg tagcaggatt aatttaagag
    32761 agaaagaaat gtttactatc aacgcagaag tacgtaaaga gcagggtaag ggtgcgagcc
    32821 gccgcctgcg tgccgctaac aagttcccgg caatcatcta cggtggcaaa gaagcgccgc
    32881 tggctatcga gctggatcac gacaaagtca tgaacatgca agctaaagct gaattctaca
    32941 gcgaagttct gaccatcgtt gttgacggta aagaaatcaa agttaaagct caggacgtac
    33001 agcgtcaccc gtacaaaccg aagctgcagc acatcgactt cgttcgcgct taattgctga
    33061 ataagttgta aaaaaccccg ctccggcggg gttttttgta tctgcagatt atgcctgatt
    33121 acggtattgc tattttttgc aggccagata aggcgttcac gccgcatctg gcattcagaa
    33181 cacaaaaccg attaattgcc gccagacgtc cggcgctgca attggtcgcg caaattcggc
    33241 ggcgtgcctt taatggtcag ggtatcggtc gccggctccc agaagatgcg ttcacctaac
    33301 agcatcgcat caaagttaat agtcaagcca ccgccgctac cggcaaactt cgtcaactga
    33361 cgcagtgtgc tccggtctgc cgggaagctc tcttccagtt cgtagccttt ttcagccgca
    33421 aactccgtga agctcacttc gcttacgcct gccagctctt tcgacagcga ttccagttcg
    33481 atctcttcgc ctgcctgcag ctgctcatta cagtagctat aaacctgctg gcgcacgttc
    33541 tgtcgctctg ctttatccag ttgtgcttcg gcagtgaaat catcaaccgc ttgcaacagc
    33601 ccacggtttt gcgctttcgt gttcaatcct tcgctggccc cgaggaaatc cataaagaaa
    33661 tccgccactt tgcgccctac tcgccctttc aggaaagtga gatagcgggt ggactctgga
    33721 ttggtttccc attcggttaa atcgatacgc gcaacaatat ccgcatgatt gatatcaaga
    33781 taatgggttg ggttgatgtc gagattttcg ttaacacgca tactgctcag gttgctcaga
    33841 actgccacca gcagatactc caccgccaga taacggtagt ggcaaaacag cacgaatcca
    33901 ccgtcagcaa aaggatattt cgctaattcg tcacgcaggc gaccagttgc cgcgcggcta
    33961 aatgccagaa agtcctcttc gccctgacgc tgtaagcgca gcgtctgcgc cagttcactc
    34021 tcttcgctaa acaagccgta agctttattt ttggcgctat agacccgatg cagttctgcc
    34081 accatctcaa cgacggtttc tgtcggttcc agcaatgaat cgcgcaacac cagctcaagg
    34141 ttctgctcat cacgcttgat aagctggtgc agagcaatct ggttgatatc cagactcatg
    34201 ataaactctc ctttaagacc gggcggtatt caaccaccgc cggttaagcg acgcaataaa
    34261 agatggcggt cattttacag aaggcagcgt aacgagaatg atttaacgca taaaaaagca
    34321 gaaaaaaaac cgttgctacg gtaatatgtt gccctttcat caactaactg atttcgattt
    34381 atgccacaaa tttcccgcta cagtgatgaa caggttgaac aattgctcgc agagctgctc
    34441 aacgtactgg aaaaacataa ggctccgacc gacctttccc tgatggtgtt aggcaacatg
    34501 gtcactaacc ttatcaacac cagcattgcc ccggcccaac gccaggcaat tgccaactct
    34561 tttgcccgcg ccttacagtc ctctatcaac gaagacaaag cgcactaagg gaaacagata
    34621 acaggttatg gtaactcatc gtcagcgcta ccgtgaaaaa gtctcccaga tggtcagttg
    34681 ggggcactgg tttgcactgt tcaatattct gctttcgctc gtcattggca gccgttacct
    34741 gtttatcgcc gactggccga caacgcttgc tggtcgcatt tattcctacg taagcattat
    34801 cggccatttc agcttcctgg tgttcgccac ctacttgctg atcctcttcc cgctgacctt
    34861 tatcgtcggc tcccagaggc tgatgaggtt tttgtccgtc attctggcaa cggcgggaat
    34921 gacgctatta ctgatcgata gcgaagtctt tactcgtttc catctccatc ttaatcccat
    34981 cgtctggcag ttggttatca acccagacga aaatgagatg gcgcgcgact ggcagctgat
    35041 gttcatcagc gtgccggtta ttttattgct tgaactggtg tttgcgacgt ggagctggca
    35101 aaagctgcgc agcctgacgc gtcgtcgacg cttcgcgcgc ccgctggccg cattcttatt
    35161 tatcgccttt atcgcctcgc atgtggtgta tatctgggcc gatgccaact tctatcgccc
    35221 gatcaccatg cagcgcgcta acctgccgct ttcgtacccg atgacggcgc gacgttttct
    35281 tgagaagcat ggtctgcttg atgcgcagga gtatcaacgc cgtcttattg agcaaggtaa
    35341 tccagacgcc gtttccgttc agtatccgtt aagcgaactg cgctatcgcg atatgggcac
    35401 cgggcagaat gtgctgttga ttactgtcga tggcctgaac tactcacgct tcgagaagca
    35461 gatgcctgcg ctggcaggtt ttgctgagca aaatatttcg ttcacgcgcc atatgagctc
    35521 cggcaacact acagacaacg gcatctttgg cctgttctat ggtatctcgc cgagctatat
    35581 ggacggcatt ctgtcgaccc gtacgcctgc ggcattaatt actgcgctta atcagcaagg
    35641 ctatcagctg gggttattct catcagatgg ctttaccagc ccgctgtatc gccaggcatt
    35701 gttgtcagat ttctcgatgc cgagcgtacg cacccaatcc gacgagcaga ccgccacgca
    35761 gtggatcaac tggctgggcc actacgcaca agaagataac cgctggttct cgtgggtttc
    35821 tttcaatggc actaacattg acgacagcaa tcagcaggca tttgcacgga aatatagccg
    35881 ggcggcaggc aatgtcgatg accagatcaa ccgcgtgctc aatgcactgc gtgattctgg
    35941 caaactggac aatacggttg tgattatcac tgccggtcgg ggtattccgc tgagcgaaga
    36001 cgaagaaacc tttgactggt cccacggtca tctgcaggtg ccattagtga ttcactggcc
    36061 aggcacgccg gcgcagcgta ttaatgcgct gactgatcat accgatctga tgacgacgct
    36121 gatgcaacgc ctgctacatg tcagcacacc tgccagcgaa tattcgcaag gtcaggattt
    36181 gttcaaccct caacgccgtc attactgggt tactgcagcg gataacgata cgctggcaat
    36241 taccaccccg aaaaagacgc tggtgctgaa caataacggt aaataccgca cttacaactt
    36301 acgtggtgaa agagtgaaag atgaaaaacc acagttaagt ttgttattgc aagtgctgac
    36361 agacgagaag cgttttatcg ctaactgatt aattataaat cagttagcga aatatcttac
    36421 ttgcaatcgg tgtggaaaac ggtagtatta gcagccacga gtcggcacgt agcgcagcct
    36481 ggtagcgcac cgtcatgggg tgtcgggggt cggaggttca aatcctctcg tgccgaccaa
    36541 aaatcccaag aaaaaaccaa cctttacggt tggttttttt atatctgcaa ttaatttgat
    36601 aaacagaccg tgacacatca cagcctgttt attttctgtt atcagaacgt ccagaccact
    36661 cccgcctgag tattccacgg cgattctacc cctgcgccat tgccatagct gactgacaaa
    36721 tgaccgctta acgtcggggt aaacgatgac cttatcccag cctgataaac gccacgggtt
    36781 ccagccacat cattattaaa tttgccatcg tcattaactt tcacatggtt agaatcggcc
    36841 tattcctgac gcacggcggc tttcagccag ggttccaacg tcgtaccgtt ttgcaggtcc
    36901 atgtgatagc ttaccgccgt tcccgcttca gcgcgtaata tccgggtatt tcccacatcc
    36961 gcgcgcatgc cgtttgataa cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcg
    37021 agataggggc taacactcca caatccgtca acccaacgga acccgctctc aacatgagca
    37081 cccgcgccgt tactattgta atcgccaaac gctgttgccc cattactcat cttgccatgg
    37141 atggtgttgg caaaacggtc aactttcacc accccatcaa cataggcacc gttctgatgc
    37201 tcccaaccgg cataagcccc cagggtgtag ctatcaatat tgcctttgcc gccgcgatca
    37261 aaaccaatat cagaatgaga gtaatcaaag aacaagccgc gaattgtgct gctttcttca
    37321 cgggagaaac ggctatcgat accgagcgtc aggcccgtca atgtttgctc aaagccagct
    37381 cccgcatcag tggtcacgtt gttgcgggtg ttaattgccg aactccacat cgccgtatcg
    37441 taattaacgc cttttacgct accaagacgc tcacgcacgg tgtccagttc tgcatcaaat
    37501 accagcggtt gtgctgctgc catgttcagc acatcagtgg ttgaaggggt aatttgcgcg
    37561 cgattctctg cgaggctcca gctatggttg ccattatcca gcaaggtata ttcatacgta
    37621 ccgatatcaa caacgcctcc ggcattgccc aacgtgaatg cagcatcacc gccgcccgtc
    37681 gttaccagtg taaggctatc tcctgctgcc gggctggccc ccgtgtccgt cacgaatatt
    37741 ttgaaatcac ctgttgcctg accgatgacg ttgagctgat cgctctgatg attagccata
    37801 tcggtacgca tataaaattg ccgtttcccg acagggtatt ggtggtcaac gtaatgaaat
    37861 tttccgcact tgccgtcaca gatgaacgag tcactggctc agccatcata atcacatttg
    37921 cgccggacat cgcaaggttg gctaagttga ggcgagtgtt ttggttcgtc gtagtgatat
    37981 cggcaatgat ggtccatgcg ctattttcga gcgaaacgtc cgcttggctg gtatccacgg
    38041 caccatgcgt tctaaaagaa gcgccctcag aaaccactac cttacccacc aacatagcgg
    38101 gtgcataatt tattttcggc ttcatgacct caagaatgcc atcattccct ctgactgaca
    38161 cgttaaccat tgtgccagcc cagacattag cgcggccatt gttgataacc atattttcag
    38221 agatggcttt agaggaatac gtgatacttc cattctgatc tgatcgaccg agatcatata
    38281 cagcatttgc ctgcactttc gtaccagtat cctttcccag cgattgcata gtggcatgct
    38341 catcaaggat agtgtcaatg gcctgagtgt cctccataac aatgagcccg gagccatcat
    38401 ccagttcata attttttgac acaccatcct ttatactaaa ttggccttta ctgtttgtac
    38461 cactcacttc cagcgcattc gttgagacaa ttaatttccc gcctgctttt ttatcaacgc
    38521 cagttgcagt cccgccatcc ataacctcca gtaagccgcc actatccaca gtggtattat
    38581 aagcgaagtc attttcttca actcgcaaac ttccgccgtt ttccaacagc atattgttag
    38641 caataccatt cttgatttcg aactgaccga gacggtttgt tccgaatacc tccatgaccc
    38701 gcgtggttgt tttaatagca ccgcctgctt tctgatctac cgcaaatgct aatcccccgt
    38761 ctttaacctc cagtaatcct cccttttcga tagcggcaaa gacggcaatc ccatcttttt
    38821 caacaattaa gcgcccgcct ttaatcgtgg gatcattcgc cgcg
//
LOCUS       DAZOZY010000018        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZOZY010000018 DAZOZY010000000
VERSION     DAZOZY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.018"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHURQ_000807"
     CDS             59..1423
                     /locus_tag="ACHURQ_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0822657.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHURQ_000808"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHURQ_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR0822658.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHURQ_000809"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHURQ_000809"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR0822659.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHURQ_000810"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHURQ_000810"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR0822660.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHURQ_000811"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHURQ_000811"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR0822661.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHURQ_000812"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHURQ_000812"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR0822662.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHURQ_000813"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHURQ_000813"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR0822663.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHURQ_000814"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHURQ_000814"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR0822664.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHURQ_000815"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHURQ_000815"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR0822665.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHURQ_000816"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHURQ_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR0822666.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHURQ_000817"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHURQ_000817"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR0822667.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHURQ_000818"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHURQ_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR0822668.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHURQ_000819"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHURQ_000819"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR0822669.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHURQ_000820"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHURQ_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR0822670.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHURQ_000821"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHURQ_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR0822671.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHURQ_000822"
     CDS             13749..14288
                     /locus_tag="ACHURQ_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR0822672.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHURQ_000823"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHURQ_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR0822673.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHURQ_000824"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHURQ_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR0822674.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHURQ_000825"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHURQ_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR0822675.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHURQ_000826"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHURQ_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR0822676.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHURQ_000827"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHURQ_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR0822677.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHURQ_000828"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHURQ_000828"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR0822678.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHURQ_000829"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHURQ_000829"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR0822679.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHURQ_000830"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHURQ_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR0822680.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHURQ_000831"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHURQ_000831"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR0822681.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHURQ_000832"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHURQ_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR0822682.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHURQ_000833"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHURQ_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR0822683.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHURQ_000834"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHURQ_000834"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR0822684.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHURQ_000835"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHURQ_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR0822685.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHURQ_000836"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHURQ_000836"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR0822686.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHURQ_000837"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHURQ_000837"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR0822687.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHURQ_000838"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHURQ_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR0822688.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHURQ_000839"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHURQ_000839"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR0822689.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHURQ_000840"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHURQ_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR0822690.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHURQ_000841"
     CDS             complement(37465..37920)
                     /locus_tag="ACHURQ_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822691.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHURQ_000842"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHURQ_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9466 a        10313 c         9734 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactgacgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZOZY010000019        37260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZOZY010000019 DAZOZY010000000
VERSION     DAZOZY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.019"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..645)
                     /locus_tag="ACHURQ_000843"
     CDS             complement(<1..645)
                     /locus_tag="ACHURQ_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR0822692.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLL"
     gene            complement(745..1656)
                     /gene="gsiD"
                     /locus_tag="ACHURQ_000844"
     CDS             complement(745..1656)
                     /gene="gsiD"
                     /locus_tag="ACHURQ_000844"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR0822693.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1659..2579)
                     /gene="gsiC"
                     /locus_tag="ACHURQ_000845"
     CDS             complement(1659..2579)
                     /gene="gsiC"
                     /locus_tag="ACHURQ_000845"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR0822694.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2597..4135)
                     /gene="gsiB"
                     /locus_tag="ACHURQ_000846"
     CDS             complement(2597..4135)
                     /gene="gsiB"
                     /locus_tag="ACHURQ_000846"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR0822695.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4155..6026)
                     /gene="gsiA"
                     /locus_tag="ACHURQ_000847"
     CDS             complement(4155..6026)
                     /gene="gsiA"
                     /locus_tag="ACHURQ_000847"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR0822696.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6013..6978)
                     /gene="iaaA"
                     /locus_tag="ACHURQ_000848"
     CDS             complement(6013..6978)
                     /gene="iaaA"
                     /locus_tag="ACHURQ_000848"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR0822697.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7182..8417
                     /gene="moeA"
                     /locus_tag="ACHURQ_000849"
     CDS             7182..8417
                     /gene="moeA"
                     /locus_tag="ACHURQ_000849"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR0822698.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8417..9166
                     /gene="moeB"
                     /locus_tag="ACHURQ_000850"
     CDS             8417..9166
                     /gene="moeB"
                     /locus_tag="ACHURQ_000850"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR0822699.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9345..10007)
                     /gene="fsa"
                     /locus_tag="ACHURQ_000851"
     CDS             complement(9345..10007)
                     /gene="fsa"
                     /locus_tag="ACHURQ_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR0822700.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10138..11037
                     /locus_tag="ACHURQ_000852"
     CDS             10138..11037
                     /locus_tag="ACHURQ_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR0822701.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11043..13475
                     /locus_tag="ACHURQ_000853"
     CDS             11043..13475
                     /locus_tag="ACHURQ_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR0822702.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13621..14436
                     /gene="ybiV"
                     /locus_tag="ACHURQ_000854"
     CDS             13621..14436
                     /gene="ybiV"
                     /locus_tag="ACHURQ_000854"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR0822703.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14588..15853
                     /locus_tag="ACHURQ_000855"
     CDS             14588..15853
                     /locus_tag="ACHURQ_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR0822704.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15820..16980)
                     /locus_tag="ACHURQ_000856"
     CDS             complement(15820..16980)
                     /locus_tag="ACHURQ_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0822705.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17218..18519
                     /locus_tag="ACHURQ_000857"
     CDS             17218..18519
                     /locus_tag="ACHURQ_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0822706.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18534..20897
                     /locus_tag="ACHURQ_000858"
     CDS             18534..20897
                     /locus_tag="ACHURQ_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR0822707.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21077..22672)
                     /locus_tag="ACHURQ_000859"
     CDS             complement(21077..22672)
                     /locus_tag="ACHURQ_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR0822708.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22891..23811
                     /gene="ldtB"
                     /locus_tag="ACHURQ_000860"
     CDS             22891..23811
                     /gene="ldtB"
                     /locus_tag="ACHURQ_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0822709.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23870..24988)
                     /locus_tag="ACHURQ_000861"
     CDS             complement(23870..24988)
                     /locus_tag="ACHURQ_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR0822710.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24985..25452)
                     /gene="mntR"
                     /locus_tag="ACHURQ_000862"
     CDS             complement(24985..25452)
                     /gene="mntR"
                     /locus_tag="ACHURQ_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR0822711.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25638..25766
                     /gene="mntS"
                     /locus_tag="ACHURQ_000863"
     CDS             25638..25766
                     /gene="mntS"
                     /locus_tag="ACHURQ_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR0822712.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26038..27621
                     /gene="opgE"
                     /locus_tag="ACHURQ_000864"
     CDS             26038..27621
                     /gene="opgE"
                     /locus_tag="ACHURQ_000864"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR0822713.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27670..28185)
                     /gene="ompX"
                     /locus_tag="ACHURQ_000865"
     CDS             complement(27670..28185)
                     /gene="ompX"
                     /locus_tag="ACHURQ_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR0822714.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28238..28303)
                     /locus_tag="ACHURQ_000866"
     CDS             complement(28238..28303)
                     /locus_tag="ACHURQ_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822715.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28538..29425
                     /gene="rhtA"
                     /locus_tag="ACHURQ_000867"
     CDS             28538..29425
                     /gene="rhtA"
                     /locus_tag="ACHURQ_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR0822716.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29724..30227
                     /gene="dps"
                     /locus_tag="ACHURQ_000868"
                     /gene_synonym="pexB"
     CDS             29724..30227
                     /gene="dps"
                     /locus_tag="ACHURQ_000868"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR0822717.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            30631..31377
                     /gene="glnH"
                     /locus_tag="ACHURQ_000869"
     CDS             30631..31377
                     /gene="glnH"
                     /locus_tag="ACHURQ_000869"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR0822718.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            31516..32175
                     /gene="glnP"
                     /locus_tag="ACHURQ_000870"
     CDS             31516..32175
                     /gene="glnP"
                     /locus_tag="ACHURQ_000870"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR0822719.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            32172..32894
                     /gene="glnQ"
                     /locus_tag="ACHURQ_000871"
     CDS             32172..32894
                     /gene="glnQ"
                     /locus_tag="ACHURQ_000871"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR0822720.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            33011..35236
                     /gene="ybiO"
                     /locus_tag="ACHURQ_000872"
     CDS             33011..35236
                     /gene="ybiO"
                     /locus_tag="ACHURQ_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR0822721.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(35233..36159)
                     /gene="rlmF"
                     /locus_tag="ACHURQ_000873"
     CDS             complement(35233..36159)
                     /gene="rlmF"
                     /locus_tag="ACHURQ_000873"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR0822722.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            36435..36695
                     /gene="mcbA"
                     /locus_tag="ACHURQ_000874"
     CDS             36435..36695
                     /gene="mcbA"
                     /locus_tag="ACHURQ_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR0822723.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            36960..>37260
                     /locus_tag="ACHURQ_000875"
     CDS             36960..>37260
                     /locus_tag="ACHURQ_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0822724.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         9035 a        10058 c         9337 g         8830 t
ORIGIN      
        1 taacaaatcg tgatctttgc cgttgatacg tagcgtaaaa gagatacatt ttttacccgg
       61 acgatcaaag caattgtatg ccggattcca gctgttgtta gagtcatctg aagccaccga
      121 ccacggtcgt ttcgatgggt taaaatcggc aaaaataacc cgccctgact cgtcgacaag
      181 cggtccattt acttgttcct gatgataaac aacgatttta tcattcgccc tccgcgaaaa
      241 gagataatat ttattatcat caagtaccag tcccaccgag tctaaaaatg gcatcatttc
      301 cattttcaga atcacggcgc gttgaatctc ctctgtattt tttaataaat acagtgagtc
      361 ctttaatgtc agggccgaac gttcaatatc tttaaagaaa tagccaacgt ttttctgaat
      421 aagttcaaag cggctatcgg cacgttttac cgcgcgttcc gataccagtt gatagttaat
      481 gatgaaaaaa agaccaataa atgacagtgc tgccaataaa aataaaatta tcagccttat
      541 ctttatcttt tcgtataaac tcagcatgtt aatacattta acaaaataac tatctgaacg
      601 ctgtgccgct gcaaaaatag tcccttgctg cggcttttta accaaattac ccgattctac
      661 aaaatcagca gaaagttaca acacttgctt agggcaataa tttcctgtga ggtgattacc
      721 ctttcaagca atattcaaac gtaactatcc tttaattttc ggatccagcg catcgcgtaa
      781 accatcgccc aacaaattga acgccagtac ggtcagaaaa atagccaggg ccggaaaaac
      841 agcgacatgc ggcgcgataa ccatatccgc tcgtgcctca ttgagcattg ctccccactc
      901 tggtgtcggc ggctgcgcac cgaggccgag aaatgacagg ctggcggcag agataatcga
      961 ggtgccaatg cgcatggtga aaaacaccac gatagacgag acggtcccag gcaagatatg
     1021 acgcaataaa atggtcatat cgctggcacc aatactgcgt gctgactcaa taaaggtttg
     1081 ctgtttcaac accagcgtgt tgccgcgcac cagtcgggca aacgcgggga tggaaaaaat
     1141 ggcgactgcg ataatcacgt tagcaatgcc gcttcccaac accgcaacaa cagcgatcgc
     1201 cagtaaaata cccgggaagg caaacagcac atcgcaaatg cgcatgatca gccgatccca
     1261 ccagccttca taatatccag ccaacaagcc cagcaacgtc ccgatcgccg caccgataaa
     1321 cacggcaaac acgcccgccg ccagcgagat ttgcgcacca accaggacac ggctgaaaat
     1381 gtcgcgcccc agtgaatcga cgccaaacca gtgctgcaaa gaaggtccgt tattcagatt
     1441 gtcataatca aaataatttt cggcgtcata gggagcgatc cagcgtgcaa aaatggccac
     1501 cacaatcaat aaaataacga ataatgcggc ggtcatcgcc atatgctgac ggcgaaatcg
     1561 tcgccagaat tcatgccacg gtgtacgcac ctggtcaggt ttgaccagtg gcatggcgtt
     1621 taacaccgcc tgacgtcgcc agttaaatag tcgcatcctt acttgtacct gatagccggg
     1681 ttaatggcgg cgtaaagcac atccaccact aagttgataa gaataaattc cagcgagaaa
     1741 agcagaattt ccgcctgaat caccgggtaa tcgcgcattt ctacggagtc aacgagtaag
     1801 cgtccaagtc cgggccagtt gaaaactttc tccaccacga tagaaccacc gagcaaaaag
     1861 ccaaactgta agcccatcat ggtcactacc gggatcatcg cgttacgtag cccgtgtttg
     1921 aggacaaccc aggtttcgct cacccctttc gccctcgcgg tacgcatata atcttcgctt
     1981 aaaacatcga caaacgacgc gcgggtaaag cgcgccatca cggctgccac tgccgcgccg
     2041 agcgtcaggg agggtaaaat gtagtgctgc caactgtctg ctcccacggt aggcagccag
     2101 cccagttcaa cggagaatac ctgaattaaa agcatcccca gcgcaaatgc cggaaacgag
     2161 atccccgaca ccgcaatggt catactcaat cgatccggcc aacgattacg ccagacggcg
     2221 gcgataattc ccgccgccat accaaatatc accgcccaga ccatactggt tatggtcagc
     2281 cacagcgttg gcataaagcg gctggcaatc tcatcggcaa ccggacgacg cgacaccatc
     2341 gacaggccaa aatccccctg cacagcattg ctgatatagt gccagaactg gtgatacagc
     2401 ggctgatcca accccagctg ctgacgcacc agttctataa cctgcgcatc agcttcgagc
     2461 ccggcaatca atcgcgccgg atcgccgggc agcatatgca caaataaaaa caccagcacc
     2521 gagacgataa acagcgtcgg aatcaacccc agtaagcgtt tgataacgta attaagcatt
     2581 ccactccctg cgttgcttat tgcaaatccg cgtcttcaaa gctgaagccg gtgtctggca
     2641 tgatccaaaa accggtcagg tttttactgt gtgccgacac cagtttttct accaccagcg
     2701 ggatccacgg cgactcttgc cagatgatat cctgcgccgc cttatacaag cgggtctttt
     2761 ccgccggatc attagttttc agtgcctgag ccaggaagtc gtccacctgt ttattgctgt
     2821 aaaatgcggt attaaacagc gtcggtggcc agttttgcga ggcgaacagt ggcgatagcg
     2881 cccagtccgc ttcgccggtt gaagccgacc agccagtgta gaacatccgc acgccgctct
     2941 ctttttgccc tttaccttca acttctgccg cccgctgtcc ggcatccatc gcggtcacct
     3001 gggctttaat cccgacctgc gctaactgct gctgggtaaa ttgcagcact ttctgcgcgg
     3061 tgctgtggtt atgtgacgac cacagcgtgg tactgaaacc gttgggatat cccgcctctt
     3121 tcagtaattc gcgcgctttc actggatcgt aaggccacgg tttataactt tgcgcgtagg
     3181 cgatacttgg cggtaccaca ccagtagctg gcgttgcata gcccgcaaag gcaactttca
     3241 ccagcgccgg acggttaatg gcgtaattca gcgcctcacg gaccttcggg ttatcgaacg
     3301 gcttttgcgt cacgttcata ctgatataac gctgcataat tgacggactg gccatcaact
     3361 cgatattttt gtttttctcc agcagtgtgg cctgctcgta gggaatgggg aaagcaaact
     3421 gcgcttcacc ggtttgcagc attgccgcgc gggtgttgtt atccgccacc ggacgccagg
     3481 ttatgctgtc cagtttgggc aatcctggct gccagtaacc cgcgaatttt ttcaccttca
     3541 caaaatcggt ctgattccag gtatccagtt catacggtcc ggttcccacc ggatgaaaac
     3601 caatctcctt gccatatttt tccagcgctg ccggtgaaat catcgcggtc gccggatggg
     3661 caagaatatt aataaacgct gagaacggct gtttgagggt aatctttacc gttgtcggat
     3721 cgatcgcctc cgttttggca atattcttat acaggttata gcgtttaaga tgattcgccg
     3781 gatcgctggc ccggtccaga ttcgctttta ccgccactgc gttgaaatcg gtgccatcct
     3841 ggaatttaat gccttcccgc aatttcacgg tgtaagtaat gccgtcatcg gaaacggtat
     3901 aactctccgc cagcacgttt ttcagtttca tctctttatc cagaccgaac agcccctggt
     3961 aaaacgattt cgctacggcc tgagataacg tgtcattcgc gtcatacgga tcgagcgtgg
     4021 tgaaattcga tcctaccgcc accaccacat ctttggcagc gaatgcacaa gatgccatca
     4081 acgctgtcgc aatgcccagc gccactaacc cactacggtg tacagctctt gccattttat
     4141 tctccgcctg aatgttatct acgcatgaat gcgtattctg attgtggttg tgcgacgtaa
     4201 tgccccggcc cgacgcattg caacgagacg gctgccacct cttcgccacg cagatgaata
     4261 ttgctgggaa gatcgtccga cagcagtaca cgctgcggtc gttgtcggga cggttcagcg
     4321 accggaactg ccgccagtaa tttacgcgta taaggatgct gcgggttttc gaagaccgcg
     4381 cgccgtggac caatttcaac aatttgcccg agatacatca ccgccacacg atgactaatc
     4441 cgctctacca cggccatatc gtgggagata aacagatacg caatgccgaa atcacgctgg
     4501 agatcgagca acaagttgat aatctgcccg cgaatagaaa catccagcgc cgaaacggct
     4561 tcgtcggcaa tgatcacttt tggattcaat gccaacgcgc gagcaatgca gatgcgctgg
     4621 cgctgaccgc cggaaaactc atgcgggtaa cgccaggcat gttcaggtaa caggcccacg
     4681 cgctccagca accacgcaac gcgtgcagcc gcgtctttac ctggcaataa accgtgtaca
     4741 cgcagcggtt cgataatcga atcaccgatg gtctgacgtg ggtccagcga agcgtaaggg
     4801 tcctgaaaaa taaactgaat atcccggcgc aatgcctgaa gtttgccggg tgacaaggta
     4861 tcgattctct gaccgttaaa gataatttcg ccgccctgcg attcgaccag gcgcagcaac
     4921 gcccgcccgg tagtggattt accgctgcca gactcgccca ccagcgatag cgtttcgcca
     4981 ggccagagat caaaactgac tttctcaacg gcatgcactt cccgcgttac gcgattcaac
     5041 aaaccgctgc gcaaagggaa acgggtgaca agattacgca ctcgtaaaac aggttcgcca
     5101 tccaccaccg ttttctgctc gatggggggg gcctgtttcg ctggatgttc aagcgatatc
     5161 aacgggaaac gtcggggata atccaaccct ttcatcgcac caagttgcgg aacagcagct
     5221 aacagcgcac gggtgtaagg atgttgcggt gcatgaaaaa tctgttcgac ggtacccgtt
     5281 tccaccgcct cgccctgata catcaccagt acccgatcgg caatctctgc caccacgccc
     5341 atatcgtgag tgataaagat aacgcccatc gacatctctt tttgcaatac tttgattaat
     5401 tgcaggatct gcgcctgaat agtgacatcc agcgcggtgg ttggctcatc ggcaatcagc
     5461 accgccgggc ggcatgacag cgccatcgca atcatcactc gctggcgcat cccgccagag
     5521 agttgatgcg gataacgtga aagaatggtt tgtgcctcag gaatgcgtac ctgatccagc
     5581 atccgcttcg cctcgaccat cgcttcttca cgactggcgt tctgatgcag acgaattgat
     5641 tcggcaatct gttcacccac agtaaatacc gggttcagcg atgtcatcgg ctcctgaaat
     5701 atcatcgcca tatccgcacc gcgaacatgg cgcatttgtg cagcgctctg ctcgctaagt
     5761 tcaatcactt cgcgactgcg ccgctgcaac agcattttat cgcactgtac taaaccgccc
     5821 gcctgttcca acaggcgcat caatgccaac gcagtcactg acttaccgga gccggattcg
     5881 ccaacaattg ccagcgtctc accgcgttgc agactaaaag agagattgcg gaccgcagct
     5941 attttctgct ggtcctgcat aaaggcaata ttcagatttt caaccgccag cacattaccg
     6001 gcatcaagtt catcactgtg tggcaacggt gtcccctttt tcacggtaga taccggtggt
     6061 tggcgtatcg cctgcgtagc cccaggcgcg atacattcct tcggtgttaa acggtagcgc
     6121 gacattccct tcatggtcga tagcgattaa gccaccgcta ccgccaagcg cagggagttt
     6181 ttccattact acccgctcgc aggcttccgc gagacttaat ccgccgtaat ccattaacgc
     6241 ggcgatgtca tatgccgcca gcgcgcggat gaagacttcg cccgtgccga tacaagaaac
     6301 cgccacactg gcgttattgg cgtagcatcc ggcacccact aagggactat cgccaactcg
     6361 tccgggtaat ttattggtca ttccgcctgt ggacgtggct gccgccaaat tgccgtctaa
     6421 atccaacgcc acggccccca cggtgcccat tttctgtttt tcatccagtg gcgcaccgct
     6481 atggtcgagg acggttgccc cttccttgcg cgctgccagt agttgttcat aacgcaaagg
     6541 cgtggagaaa atctccggcg agacgcgctc catgccacga gcaaacgcaa aattttctgc
     6601 cccttcgcca atcatcatca catgcgggct ttgctccatc accagccggg cggcaagaac
     6661 cggattacgc agatgactaa cgcccgccac cgcaccggct ttcagggtgt taccatccat
     6721 cacacaggcg tccagttcat gggtttcatc acgcgtaaag acagcgccaa ttccggcgtt
     6781 aaacagtgga cactcttcca gcagacgcac cgcttccgtc accacatcca gcgcactttc
     6841 gcccgcttcc agcattttct gcccggtttc aacaatggca gacaacgcct caatgtagcg
     6901 taattcctgt tgcagactca tctgcgcgcg gctaattgca cctgcgccac catgaattgc
     6961 aatgactgct ttgcccatag tattcgtcca ttaagggtgg ctcgccactt ttgctataaa
     7021 tatcagaatt tttatgaatt actcattcaa tttttgaata tagaaagatg gatatggcat
     7081 gtaaaggcag gagccaacgc tacgcccgag gtggcataca gtctgtggcg tttttctgac
     7141 ataataggca aattcgattt tgcctccgca ggagtgtttt catggaattt accaccggat
     7201 tgatgtcgct cgacaccgcg cttaatgaga tgctttctcg cgtcacccca ctgaccgccc
     7261 aggaaacgct gccactggta cagtgttttg gtcgtattct ggcgagcgat gtcgtttcgc
     7321 cgcttgatgt tccggggttt gataactccg caatggacgg ctacgcggtg cgtttagccg
     7381 atattgcctc cgggcaaccg ctgcccgttg ccggtaaatc ctttgccggt cagccgtatc
     7441 atggtgaatg gcctgcgggc acctgcattc gtattatgac cggtgcgccg gtgccggaag
     7501 gctgcgaagc ggtggtgatg caggagcaga ctgaacaaac ggacaatggc gtgcgtttta
     7561 ctgctgaagt gcgtagcggg caaaatattc gccgtcgcgg tgaagatatc tctgcaggtg
     7621 cggttgtttt cccggcggga actcgcctga ctaccgcaga gctgccagta attgcttcac
     7681 tggggattgc cgaagttccg gtgattcgta aagtgcgtgt agcgcttttt tctaccggtg
     7741 atgaactcca gttgcccggt cagccgctgg gcgacggcca aatctacgac accaaccgtc
     7801 tcgccgtaca cctgatgtta gaacagctgg gttgcgaggt aatcaactta gggattatcc
     7861 gcgacgatcc ccatgccctg cgcgccgcat ttattgaagc cgacagccag gcggatgtag
     7921 tgatcagttc cggcggtgtt tcagtgggtg aagcggatta caccaaaacg attcttgaag
     7981 agctggggga gatcgccttc tggaagctgg cgattaaacc aggtaaaccg ttcgcgttcg
     8041 gtaaactcag caatagctgg ttctgcggcc tgccgggcaa cccggtttca gcgacgctga
     8101 ccttctatca actggtacag cctttgctgg caaaactaag cggcaacacc gccagcggcc
     8161 tgcccgcgcg ccagcgcgta cgcacagcgt cccgcctgaa aaaaacgcca ggacgtcttg
     8221 atttccagcg cggcgtgctg caacgcaacg ccgacggtga acttgaagtg acgaccaccg
     8281 gacatcaggg ttcacatata tttagctcct ttagtctcgg caactgcttt atcgtgctgg
     8341 aacgcgatcg cggcaatgtg gacgtgggcg aatgggtgga agtagaaccg tttaacgcgt
     8401 tgttcggagg cctgtaatgg cggaactcag cgatcaggag atgctgcgct acaaccggca
     8461 aatcattctg cgcggttttg attttgacgg tcaggaggcg ctaaaagatt ctcgtgtgct
     8521 gatcgtcggc ctcggtggac tcggctgcgc agcttcgctg tatctggcaa gcgccggtgt
     8581 cggcaacctg acgctgctcg acttcgacac ggtttcgctc tcgaatctgc aacgccagac
     8641 actgcacagt gatgccacgg tcgggcaacc gaaggtggaa tccgcccgtg acgccctgac
     8701 gcggatcaac ccacatatcg cgattacgcc agtcaatgca ctgctagatg acgcagaact
     8761 tgcagcattg attgctgaac acgatctggt gctcgactgt acggataacg ttgcggtacg
     8821 taatcaactg aacgcaggct gttttgccgc gaaggtgccg ctggtttccg gcgcggcaat
     8881 tcgtatggaa gggcaaatca cggtcttcac ttatcaggac ggcgaaccgt gctatcgctg
     8941 ccttagccgt ttgtttggcg agaacgcatt aacctgcgta gaagcaggcg tgatggcacc
     9001 gttgatcggc gtaattggtt cgttgcaggc aatggaagcg atcaaactgc tggcaggtta
     9061 tggcaaaccg accagcggga aaatcgtcat gtacgatgcg atgacctgtc agtttcgcga
     9121 aatgaaactg atgcgcaatc cggggtgtga ggtgtgcggg cagtaattgc cacctccagg
     9181 cctgataaga cgcgacaagc gtcgcatcag gcatttgctc cagacgccag atgcggcgtg
     9241 aacgccttat ctggcctacg gttcgggcac agatttccaa gcgcggcagg catcggtgca
     9301 ctccgccacg tcatcagcaa tttatgagga tgcagaacgt ccggttaaat cgacgttctg
     9361 ccaaacgctc cctgccagtc ctgctcaaac ttcgccacag cggcttccac cgccggatag
     9421 ctaatcatct gttgtgccac atccagtggc agagtaattg attcacatcc tgccagtaag
     9481 cagtccagcg cctgacgcgg ggttttgaaa ctcgctgcca gcactttcgc ctgcggcgca
     9541 tgcattttca ataactggtg taagtcggtc acagtctgaa tgccgctacc gccctgagca
     9601 tcaatacgat taacgtaagg cgcaacatat tccgcacctg ccagcgccga cagcaaccct
     9661 tgtgctgcgc catataccgc ggttcccagc gttggaatcc cttccgcttt taacatctta
     9721 atagctgcca gcccctcggc ggtcaccgga actttcacca cgatatccgc aataatagaa
     9781 cgcaatttac gtgcgtcatt caccatccct tcagcagtgg tagccattac ctgggcaaac
     9841 agacgcccct gaccgcccat cgcttcatga agttgcggaa gcacaacatc cagcggtttt
     9901 ttacccgcgg cgataatgct tgggttagtg gtcacacccg ccagcggaaa aatacgtgac
     9961 agcgccttca ccgcaacaac gtctgaagta tccagataca gttccataac catcctcaaa
    10021 atgtactttt tgtctgaatg tagactatca cgggaaaact ctctgaacag ttgacgcaca
    10081 tcaatctaac tttcattcga aagtatttta atgtttgaac gaaacagaga ggcaactatg
    10141 attttcaata ttcagcgcta ctcgacccat gatggccccg gtatccgcac ggtcgtattt
    10201 cttaaaggct gttcgctggg ctgccgctgg tgtcagaacc cggaaagccg cgcccgcacg
    10261 caggatctgc tgtatgacgc acgactgtgt ctggaaggct gcgagctgtg cgctaaggcc
    10321 gcgccggaag tgattgagcg cgcgctgaat ggtttgctta ttcatcggga aaagttaacc
    10381 ccggagcatc tgacggcgtt aaccgactgc tgtccgacac aggcattaac cgtgtgtggt
    10441 gaagtgaaaa gcgttgagga gatcatgacg accgttctgc gcgataaacc gttttacgat
    10501 cgcagcggcg gcggtttaac gctttcgggt ggtgagccct ttatgcagcc ggaaatggcg
    10561 atggcgctac tgcaagccag ccacgaggca ggcattcata ctgcggtaga aacctgtctg
    10621 catgtgccgt ggaaatatat cgccccttct ctgccctata tcgatctgtt tcttgccgat
    10681 ttaaaacacg ttgccgacgc gccgtttaaa cagtggaccg acggtaacgc cgccagagtg
    10741 ctggataacc tgaaaaaact cgccgcagcg ggcaaaaaaa tcattatccg cgtgccgctg
    10801 attcagggct ttaatgccga cgaaacctct gtaaaagcta ttaccgattt tgccgccgac
    10861 gagctgcacg ttggcgaaat tcatttcctg ccctaccaca cgctaggcat caacaaatat
    10921 cacttactta atctgcccta tgacgccccg gaaaaaccgc ttgatgcgcc agaactgctc
    10981 gactttgccc agcagtatgc ctgccagaaa gggttaaccg cgaccttacg aggataacaa
    11041 ccatgaccac actgaaactg gacacgctca gcgaccgcat taaagcgcac aaaaatgcgc
    11101 tggtgcatat tgtgaaaccg ccagtctgta ccgagcgcgc gcagcactat accgagatgt
    11161 atcaacaaca tctcgataag ccgatcccgg tacgtcgcgc gctggcactg gcgcatcacc
    11221 tggcgaatcg caccatctgg atcaaacacg atgagttgat cattggcaac caggcaagcg
    11281 aagttcgcgc cgcgccgatc ttcccggaat atactgtctc gtggatcgaa aaagagattg
    11341 atgatctggc agatcgtccc ggtgctggct ttgcggtgag cgaagagaac aaacgcgttc
    11401 tgcatgaagt gtgcccgtgg tggcgcggtc agaccgtaca ggatcgctgc tacggcatgt
    11461 ttaccgatga gcaaaaaggt ctgctggcga ccggaatcat taaagcggaa ggcaatatga
    11521 cctccggcga tgcgcacctg gcggtgaatt tcccgctgct gctggaaaaa ggtcttgatg
    11581 gtctgcgcga gaaagtagcg gaacgtcgct cgcgcatcaa tctgacggta ctggaagatt
    11641 tgcacggcga acagttcctg aaagcgattg atatcgtgct ggtggcagtc agcgaacaca
    11701 ttgaacgttt tgcagccctg gcgcgtgaaa tggccgcgac cgaaacccgc gaaagccgtc
    11761 gcgatgaact gctggcgata gcagaaaact gcgatcttat cgcccaccag ccgccgcaga
    11821 ctttctggca ggcgctgcaa ctgtgttact tcatccaatt gatattgcag atcgaatcta
    11881 acggtcactc ggtatcgttt ggtcgtatgg accagtatct ctacccgtac tatcgccgcg
    11941 acgttgaact caaccagaca ctggatcgcg aacacgccat cgaattgctg catagctgct
    12001 ggctgaaact gctggaagtg aacaagatcc gctccggctc acactcaaaa gcctctgcgg
    12061 gaagtcctct gtatcagaac gtcaccattg gcggacaaac tctggttgat ggtcaaccaa
    12121 tggacgcggt gaatccactc tcttacgcta tcctcgaatc ctgcggtcgc ctgcgttcca
    12181 ctcagcctaa cctcagcgtg cgttaccatg caggaatgag taatgatttc ctcgacgcct
    12241 gcgtacaggt gatccgttgc ggcttcggga tgccggcgtt caacaacgac gaaatcgtga
    12301 tcccggaatt tattaaactc ggtattgaac cgcaggacgc ttacgactac gcagcgattg
    12361 gttgtatcga aaccgccgtc ggtggcaaat ggggctatcg ctgtaccggc atgagcttta
    12421 tcaacttcgc ccgcgtgatg ctggcggcgc tggaaggcgg gcgtgatgcc accagcggca
    12481 aagtgttcct gccacaagaa aaagcgttgt cggcaggcaa ctttaacaat ttcgatgaag
    12541 taatggacgc gtgggatacg caaatccgtt attacacgcg taaatcaatc gaaatcgaat
    12601 atgtcgtcga caccatgctg gaagagaacg tgcacgatat tctctgctcg gcgctggtgg
    12661 atgactgtat tgagcgagcg aaaagtatca agcaaggcgg cgcgaaatat gactgggttt
    12721 ctggcctgca ggttggcatt gccaacctcg gcaacagcct ggcggcagtg aagaaactgg
    12781 tatttgaaca gggcgcgatt ggtcagcaac agctggctgc cgcactggcg gatgacttcg
    12841 atggcctgac tcatgagcaa ctgcgtcagc ggctgattaa cggtgcgccg aagtacggca
    12901 acgacgatga taccgtcgat acgctgctgg ctcgcgctta tcagacctat atcgacgaac
    12961 tgaaacagta ccataacccg cgctacggtc gtggtccggt tggcggcaac tattacgcgg
    13021 gtacctcgtc tatctccgct aacgtaccgt ttggcgcgca aactatggca acaccggacg
    13081 ggcgtaaagc ccacaccccg ctggcagaag gcgcaagccc ggcctccggt actgaccatc
    13141 ttggccctac tgcggtcatt ggctcagtgg gtaaactgcc tacggcagcg attctcggcg
    13201 gcgtgttgct caaccagaaa ctgaatccgg caacgctgga gaacgaatct gacaagcaga
    13261 aactgatgat cctgctgcgt accttctttg aagtgcataa aggctggcat attcagtaca
    13321 acatcgtttc ccgcgaaacg ctgctggagg cgaaaaaaca tccggatcag taccgcgatc
    13381 tggtagtgcg tgtcgcgggc tattccgcct tcttcaccgc gctctctcca gacgcacagg
    13441 acgatatcat cgcccgtact gaacatatgc tgtaatctcc tcggcccggc ggcgtcgtcg
    13501 ccgggctaaa tcattcacat catcaatttc atccttactt tcattcgaaa tataatttgt
    13561 gctctgcgtc acattgttat tacactgtta cggtttcatt ttcgagccag gagcacacct
    13621 atgagcgtaa aagttatcgt cacagacatg gacggtactt ttcttaacga cgccaaaacg
    13681 tacaaccaac cacgttttat ggcgcaatat caggaactga aaaagcgcgg cattaagttc
    13741 gttgtcgcca gcggtaatca gtattaccag cttatttcgt tctttcctga gctaaaggat
    13801 gagatctctt ttgtcgcgga aaacggcgca ctggtttacg aacatggcaa gcaactgttc
    13861 cacggcgaac tgacccgaca tgaatcgcgg attgttattg gcgagttgct aaaagataag
    13921 caactcaatt ttgtcgcctg cggtctgcaa agtgcatatg tcagcgaaaa tgcccccgaa
    13981 gcatttgtcg cactgatggc aaaacactac catcgcctga aagctgtaaa agattatcag
    14041 gagattgacg acgtactgtt caagttttcg ctcaacctgc cagatgaaca aatcccgtta
    14101 gtgatcgaca aactgcacat agcgctcaat ggcattatga aacccgtcac cagtggtttt
    14161 ggctttatcg acctgattat tcccggtcta cataaagcaa acggtatttc gcggttactg
    14221 aaacgctggg atctgtcacc gcaaaatgtg gtagcgattg gcgacagcgg taacgatgcg
    14281 gagatgctga aaatggcgcg ttattccttt gcgatgggca atgctgcgga aaacattaaa
    14341 caaatcgccc gttacgctac cgatgataat aatcatgaag gcgcgctgaa tgtgattcag
    14401 gcggtgctgg ataacacatc cccttttaac agctgacctc tctaccggag ccgtgcgctc
    14461 cggttttccc ttctttttac atcctgtaca tcacatcaaa tcattaaact tgcattaact
    14521 tatttataac ttatattgtc gcatgcaaat cagaatggtt tcgaatgaaa gttaatgaga
    14581 ggtggttatg gcttctactt ttaccagcga cacattgcct gccgatcaca aagcagctat
    14641 ccgtcagatg aagcacgcgc tgcgggcgca gcttggcgac gtccagcaga tctttaatca
    14701 gctaagcgat gacattgcca cgcgagtggc tgaaatcaac gcactcaaag cacagggcga
    14761 tgccgtctgg ccggtgctgt cttatgccga tatcaaagca ggtcatgtta ctgcagagca
    14821 gcgcgaacag attaaacgtc gcggttgtgc ggtgataaaa ggccatttcc cccgcgaaca
    14881 agcgctaggc tgggatcagt cgatgctgga ctatctggac cgcaaccgct ttgacgaggt
    14941 ctacaaaggc cccggcgata atttcttcgg gacgctcagc gcttcacgtc ccgagattta
    15001 ccccatctac tggtcgcagg cgcaaatgca ggcccgccag agtgaagaaa tggcgaatgc
    15061 gcagtcgttt ctcaatcgtc tgtggacatt tgaaagtgat ggaaagcaat ggtttaaccc
    15121 ggatgtgagc gtcatctacc ctgaccgtat ccgccgccgt ccgcccggaa cgacctccaa
    15181 aggtcttgga gcgcataccg actccggggc gctggaacgc tggctgcttc cagcgtatca
    15241 gcgcgttttc gccaacgtct ttaatggcaa tctggcgcaa tatgatccct ggcatgcggc
    15301 acatcgtacg gaagttgaag agtacacggt ggacaacacc accaaatgtt ccgtgtttcg
    15361 gacattccaa ggctggacag cgctctctaa tatgctgcct ggtcaggggc tgctgcacgt
    15421 tgtgcccatt cctgaagcca tggcgtacgt actgttacgt ccgctgcttg atgatgtgcc
    15481 ggaggatgaa ctgtgcggcg tagcgcccgg aagagtgttg ccggtatcag agcaatggca
    15541 tccactgtta attgaggcgt taaccagcat tccaaaactc gaggccggag actccgtctg
    15601 gtggcactgc gacgtcatcc attccgttgc ccccgttgaa aatcaacagg gttggggcaa
    15661 cgtgatgtac attcctgcgg caccaatgtg cgagaaaaat cttgcctacg cgcacaaggt
    15721 gaaggccgca ctggaaaaag gcgcatcgcc gggcgacttc ccgcgcgagg actatgaaac
    15781 aaactgggaa ggacgcttta cgcttgccga cctcaacatt cacggtaagc gagcgttggg
    15841 aatcgacagc tgagtgtcgt acagcccttc ccgttccacg gcggtgttcc cttttcctgg
    15901 gcgaacccgc cgcactgact cccgcaccgc caatcgtccg tgcagcagca gcgagcccac
    15961 cggagcatcc gggcgaatca gccgctgttg caacatttgc accgcttcat tgcctagctc
    16021 atcgcgtggc acatgcacag ccgtcagggg aacatcctcg attgccgcaa ggttaaagcc
    16081 gtcgatactc atcaccgaga catcctgtgg tacgcgcaga ccacgacgat gtagcgcatt
    16141 taccgttccc gccgccataa aatccccgcc gaccagaaac gccgtcggca acgtcttgcc
    16201 attggcggca tccagccacg ctccgacctt ttcttccgct tctttagcgc taaaactgct
    16261 gatattcagc aaatgacggt catggtgaaa ggccagatta tgctgacgcc atgattcttt
    16321 aatccctgca aaccgctgct ccatagtgta gcggcgcagg cacatcacat tcatcacctc
    16381 ccggtgaccc atttcaaaca gatagcgggc ggcgaacgcg ccaattaacc gatgatccgg
    16441 cgcaatcgcc gggaggcgca ttcgttcatc acggcagttg atcagcacac aaggcttagc
    16501 gagatcggcg gccagatcat ggatgtgcgg atcgtcaatc cccagcagga tcgctgcctg
    16561 cgtatctgct tcattcatcc gcgagagaaa gagcgcggga tcgctgtcaa actcctccag
    16621 cgcgcagtaa cgcagccgca cttcatgcgg cgataacgct ttgctgatgc tctggatcac
    16681 acggtaataa aagatatcag atcgttggtc aaaagcgcgt tgcggcgcga aaataatcag
    16741 gttgttcagc aacatacgac ctgctgccat gccatccatc acgcccaact cacgcgcgca
    16801 ggccagcacc tgttcgcgag cgcgtgcact ggtattcgct ttgccagcca gcacccgcga
    16861 aaccgtactc ggcgatagcc cggtacgtgc ggcgatatcg gcgattttca gcttcttgtc
    16921 cattttgtga tctgcctcta attcggaaat gacagaattt tcatgagcaa aaaagcccat
    16981 tctgtctggc attaaatcca tttcagtaat gtatatcagt cactcatgga aaaacgtgca
    17041 caaaacccgc tttctttcgc ttttagggtc tcttactgtg aattggtaga acaacttcca
    17101 tacaaaaata atggaaaaac gaacaataaa gactttattt atcaaaaagt taaaagtggt
    17161 gtaacaactc cattctgacg ttgccgcaca aaccctatac cattctgtgg agtgcatatg
    17221 agtcagggta tcaataacga tatgactgcc agtaaaccgc gccatatcgt caaaaatctg
    17281 cgttggtgga tactggtcct ttttttgctc ggtgttactg tcaactacat cacgcgtaac
    17341 tcgctgggta ttctcgcccc cgaactgaaa agcagcctag ggattaccac cgaacaatat
    17401 tcctggattg taggtgcttt ccagctcgcc tataccctat tccaaccgtt gtgcggatgg
    17461 ctgattgacg tcattggcct caaactgggc tttatgatct gcgcgggact gtgggcactg
    17521 gcctgtctga tgcatgcggg ggcgggaaac tgggtacatc tggcgctgtt gcgcttcttt
    17581 atgggcggtg cggaagccgc ggcaacccca gcaaacgcca aaaccatcgg tgaatggttt
    17641 ccaaaatcag aacgcccgat cgccgcaggc tgggcaggcg tcggtttttc catcggtgcc
    17701 atgctggccc caccgattat ctattttgcc cacgcttcat tcggctggca gggtgcattt
    17761 atgttcactg gcatcctggc gatgctgtgg gtggtgctgt ggtgggcgtt ctatcacagc
    17821 ccggataagc atcctaacct cagcaaagcc gagctggact ttattcgcca ggataacgaa
    17881 gcgcccccgg tcaagttacc ctttctgacc gcactgaaaa ccgtgggcaa aaataaacgc
    17941 ttttacggta tcgctatccc ggcctttatg gcagaaccgg cgtgggcggt gatgagtttc
    18001 tgggtgcctc tatacctgtc caaaacctat ggcatggacc tgaagcagat cgcattattc
    18061 gcctggttgc cgttcctcgc cgctgatctt ggcagtattg ccagcggcta tctgaccaaa
    18121 ctttacaccc gcctgtttgg gctgagtcgt gtgaactccg ttgtcgcatc atcggtcagt
    18181 ggcgcattct tgatggtttc gctggcggtg atggccttta ccaataaccc gtatatcgcc
    18241 atcgtcctta tctccatcgg cggcttcggt caccagatca tctcctgcat gctgagcgca
    18301 ctggtggtgg aatccttcga caaaggtcag atggccactg ttaacggcat gcgcggttct
    18361 gccgcgtgga tcgcaagttt cctgttctcc ctgattatcg gggttaccgc tgacaaaatc
    18421 ggctttaacc ccctgtttat cgccatgggc ttctttgacc tgattggcgc tttcttcctg
    18481 gtcaccttta ttgctgaacg tcgcgcaaaa cgcgcctgag agtggatgta aacatgaaaa
    18541 cgctgaaaaa ctggaccctg cacaaacagt ctgcccacca cgttgaactg ctggtcgatg
    18601 gccagcatat cctgtgcctg tacgtgctgg aagaaaatct gttccgcgtg ctgttaaaac
    18661 gcaaaggcga gctggcgcta gatcgcacct ggagcattgc tccggcgcag gatgtgccat
    18721 gggaagggcg cagccgcgaa gatctcagcg gctttagcct gcccgcgtgg acagtgatcc
    18781 agcacgacga caccgtggtt atcgccaccg agtcactgcg tgcaaccgtt caccagccgc
    18841 tgtggctgga gtggcactat cgtaacaacg aaggcgaatg gctgccgttg gtgaatgacc
    18901 gcccgaccag cgcttatctg ctgaatgccc acggcgatgg cgtggcacac tatcagagcc
    18961 gtcgtaaaga tgaacgcttt tatggcctcg gagataaagc cggcgatctg caacgcaacg
    19021 gtaaacgtta cgagatgcgt aacctcgacg cgatgggcta caacgccgtt agtactgacc
    19081 cgctgtacaa acacattcca ttcaccatca cccgccgcga tgacattagc ttcggcctgt
    19141 tttatgacaa cctcagcagc tgctggctgg atctgggcaa cgagatcgac aactaccaca
    19201 ccgcctatcg ccgctggcag gcggaagcgg gtgatatcga ctactacctg tttaccggcg
    19261 aacgcgtact tgatatcacc aaagccttcg tacgcctgac cggaaaaacc ctgttcggac
    19321 cgaaatggag cctgggctac agcggttcaa ctatgcacta caccgatgcg ccggacgcac
    19381 aaaatcagtt gatgaacttt atccgcctgt gtgaagaaca tgcaattccc tgcgattcgt
    19441 tccagctctc ttccggttat acctcaatta acggcaaacg ctatgtgttc aactggaact
    19501 acgacaaagt gccgcagccg aaagtgatga gccaggcatt tcacaatgct ggtttgcacc
    19561 tggccgccaa tatcaagccg tgcctgttgc aggatcaccc acgctataac gaagtggcag
    19621 aaagaggact gttcatccgt gattcagaaa cagatgaacc agaacgttcc agcttctggg
    19681 atgacgaagg ttctaacctc gactttacta atccgcagac catccagtgg tggcaggaag
    19741 gcgtgaccac acagcttctg gagatgggta tcgactccac ctggaacgac aacaacgaat
    19801 tcgaagtatg ggacggtgaa gcacgctgtc acggctttgg tcagacgatc gcgatcaaac
    19861 atattcgccc agtgatgcca ctgctgatga tgcgagcctc gatggaagcc cagcagcgtt
    19921 tcgctccgga aaaacgtccg tacttgatct cccgctccgg ctgcgcggga atgcagcgct
    19981 acgtgcagac ctggagtggc gataaccgca ctagctggga cactctgcgc tacaacaccc
    20041 gcatgggtct ggggatgagc ctctccgggc tgtataacct cggtcacgac gtcggcggtt
    20101 tctccggtga taagccggac ccggaactgt ttgtacgctg ggtacaaaac ggcgtgatgc
    20161 atccgcgctt tacgattcac tcatggaacg atgaccatac cgtaaacgaa ccgtggatgt
    20221 atccgggcgt cactccggcg atccgcagcg ctattgagtt gcgctatcgc ctgctgccct
    20281 acttttatac cctgatgtgg caggcatacg ccgatgatga accaatgcta cgtccgactt
    20341 tccttgacca cgagcacgac gttcagactt ttgaagagtg tgatgacttc ctgcttggtc
    20401 gcgatatttt ggttgccagc gtcgtagagc aaggcgagcg ccagcgccgt gtatggctgc
    20461 cagataacga aactggctgg tacgatttct acaacggcga atggttcagt ggcggtcagt
    20521 ggatcaccct cgacgcaccg ctggagaaac tgccgctgat ggttcgtgcc ggggctggtc
    20581 taccgctcag caaacgcatc acctatgtga gcgcagaaca agacgatacc cgcgaactga
    20641 aactgttccc gctgaaaggt gtgggcacaa cgtccggcct gctgtttgaa gacgatggcg
    20701 aaagctgggg ctaccaaact ggtaatgcgc tttgggtgga atgggaaatg gtttgcgatg
    20761 gggcgaccat caacctgaaa gtcaacgcgc gcggggatta tcgtcctgca tggagtgcac
    20821 tgaaagtgtc attaccattg gaagaaaaac gcacgctgct ggtcaatggt gttgaaggga
    20881 gtgaatggat gcgttaaatc attaccgggg ccgggaggcg actgcacctt acccggtcca
    20941 gccatcgaat cctttacctc agtatttcac gctgcaggaa ggcagcaaac ctgtaaaccc
    21001 cgggagctta aataagtaag tgaccggggt gaacaggtga agccaatgca tctgccgcat
    21061 gaaagacggc ggggaattaa gcgtcgatcc ctttactacg caggtaatct tcgtaattac
    21121 cgctaaagtc gatcgcgcgt tccggggtga tttccagaat gcgggtcgcc agggagctta
    21181 cgaactcacg gtcgtgtgaa acaaagatca gcgtgccctg atacagttcc agcgccatgt
    21241 tcagtgactc gatggattcc atatccaggt ggttggtcgg ttcgtccatg atcagaatgt
    21301 tcggcttctg catcattaac ttaccaaaca gcatccgccc tttttcccca ccggaaagca
    21361 ctttagctgg ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta
    21421 ccgcctgctc gtcatcgcct tcctgcttcc actgactcat ccattcgaac acggtcagat
    21481 cattttcaaa ctcatattcg tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc
    21541 attttacggt gccgctgtcc ggttgcagat cgcccaccag cgttttcagc agcgttgatt
    21601 taccgacgcc gttggtaccc agtaccgcaa gtttttcacc gacctccagc agcaggttga
    21661 gatttttaaa cagtggaccg ttatcaaacc ctttggtcag accttccact tccagcgcgt
    21721 tacggaacag tttcttatcc tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt
    21781 tcacctcttc cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt
    21841 tcgaggcgtt ggcgctaaag cggctaacga aagattgcaa ctcagcaatc tgcgctttct
    21901 tcttggcgtt atcggccagc agacgttcac gcgcctgggt cgccgccgtc atgtactcat
    21961 cgtagttacc cggataaacg cgcagctcgc cgtaatccag atccgccatg tgggtacaga
    22021 ccatgttaag gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca
    22081 gcacctgttc cagccagcga atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca
    22141 ggagaatatc cggatcagca aacagcgcct gcgccagaag cacacgcagt ttccagccag
    22201 gagcaacttc actcatcggg ccgtagtgct gttccactgg aattcccacg ccaagcaaca
    22261 gttcaccggc gcgagcttcc gcagagtaac cgtccatttc gccgtattta acttccagat
    22321 cggccacttt atagccgtct tcctcactca tttccggcaa agcatagatg cggtcacgct
    22381 cctgcttcac ttcccacaac tctttatgcc ccatgatcac cgtatccagc acagtgaact
    22441 cttcaaaggc aaactgatcc tgacgcagct taccaatgcg ctcgttggga tcgagggaaa
    22501 cgttacccaa agtcggctct aagtcgccgc cgaggatctt cataaaggtg gatttaccac
    22561 taccgttcgc gccaatcagg ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt
    22621 caaacaacgg cttactgccg aactgcatgg tgacgttact ggaaactaac acaggcgtat
    22681 cctgaaaaga gatatgacaa accgcgcatt atgccataag tctgaaagaa gatcctgctc
    22741 ctcgcttaag gtctacactc aagccaaagg caaaaaatgt gatttcgtac acatctgatt
    22801 tcactgtgag ctggaatgaa cttataatgc gcttccaata ctctaatatt ctcaacccaa
    22861 tggcctgcca ggcacaaaat ctcgcttaac atgaatatga aattgaaaac attattcgca
    22921 gcggccttcg ctgttgtcgg cttttgcagt accgcctctg cggtaactta tcctctgcca
    22981 accgacggga gtcgcctggt tggtcagaat caggtgatca ccattcctga aggtaacact
    23041 cagccgctgg agtattttgc cgcggagtac cagatggggc tttccaatat gatggaagcg
    23101 aacccgggtg tggatacctt cctgccgaaa ggcggtactg tactgaacat tccgcagcag
    23161 ctgatcctgc cggataccgt tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg
    23221 tattactatc cgaaagggac caacaccgtt atcgtgctgc cgatcggcat tggtcagtta
    23281 ggcaaagata cgcctatcaa ctggaccacc aaagttgagc gtaagaaagc aggcccgacc
    23341 tggacgccga ccgccaaaat gcacgcagag taccgcgctg cgggcgaacc gcttccggct
    23401 gtcgttccgg caggtccgga taacccgatg gggctgtatg cactctacat cggtcgcctg
    23461 tacgctatcc atggcaccaa cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt
    23521 gtgcgtctgc gtaacgaaga catcaaattc ctgttcgaga aagtaccggt cggtacccgc
    23581 gtacagttta ttgatgagcc ggtaaaagcg accaccgagc cagacggcag ccgttatatt
    23641 gaagtccata acccgctgtc taccaccgaa gcccagtttg aaggtcagga aattgtgcca
    23701 attaccctga cgaagagcgt gcaaacagtg accggtcagc cagatgttga ccaggttgtt
    23761 cttgatgaag cgattaaaaa ccgctccggg atgccggttc gtctgaatta atcttcaaaa
    23821 cttaaagcaa aaggcggact cataatccgc cttttttatt tgccagaccc tagttggccg
    23881 ggagtataac taacaaaaca tatcccacca acgccgccca caacagcatc ggtattgaat
    23941 agagatggaa acgccaccag atgcggcgat cgttcgccat acgtagcgca atcaaatttg
    24001 ccagcgatcc gggtaataac ccaaagccac ctacgtttac cgcccatacc agtaataaag
    24061 acggcggcac atagttcagc aacaatatgg tactcggcac gttactgatc acctgcgata
    24121 aaccgattgc cgttaaccat aacccgggtt cagatagatg actcacgtta cccaacacgc
    24181 cttgcaacgc tggaagctgg gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca
    24241 gcagcagcgt ccagtccaca ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg
    24301 ccacaatcac cagtccccac agctcttgtt tgaactccag cgccgtcaga aagacgatat
    24361 acagccccag acaactccac accagccgcg gtttccactc cggtgtttgc acccccgtat
    24421 ggtattgcag cgcctttcca gggaaacaac accagcacag gagcaggagc gtcagcatca
    24481 ttgcgccagc cagcggtgcc atttgggcaa taaatccggc aaacgaaaga ccagaacgtc
    24541 cccagataag aatattttgc gggttgccaa ttggcgtcag tagcgaacca gcgttgactg
    24601 ccagcgcctc aaaaataatc agccgattaa ccgggatctc acacagtctt tttagcgtga
    24661 tagtcagcgg aacaacaata aacagcgcga catcgttggt cagaaaggta gaaagcagcg
    24721 ccgccgccag caccataaac atcgccagcc gacgctccgt agcaaagcgg cgcaccattt
    24781 tgcgccccag cacatcaaaa taaccgctta actccacacc tttggtcagc agcatcaggc
    24841 cgcttaaggt gatgatggtg tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg
    24901 gcacgaaaaa gcttaatccg ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt
    24961 gcagcgtgcg taaaaaaggc aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa
    25021 agcgtcgagc gtctcttcac taacatggtg ctccatgcct tccgcgtcgc gacgggcgat
    25081 ttccggactg acgcccaaca ccaacaagaa attttcgact atctgatgtc gctcgcggct
    25141 ttcctgcgcc agcttctctc cttctgccgt taaaaacacg ccacgccagg ggatcatttc
    25201 aatcagcccc atggttgcca gccgcttaag cattttagcc accgtcggtt gcgaaactcc
    25261 cagacgagca gccatgtcca cctgacgagc ttcccccact tccctgatca agtcagaaat
    25321 cagctcaacg taatcatcaa taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc
    25381 ttcaacgtgc tcttccacgt tcactaactg cgtcactttt tttgctgttg gcgtacctgc
    25441 gcgacgactc attgtgcttc ctcattatgg tgacgcatac atagcgttct catttcgtgc
    25501 gcacattgta aaccagagtt gcgaaggtac aaaaaattaa cgttttagca atagctacat
    25561 aatatagcct gtgctatatc tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta
    25621 gcaagtcagg aggtcttatg aatgagttca agaagtgtat gcgcgtgttt agtcattctc
    25681 cctttaaagt acggttaatg ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc
    25741 agcaagataa accttccgat aaatagcggc gtcgcggtac gccgcttcac tcctgctttt
    25801 atgcaggcat aacgcgtttt ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc
    25861 ttcgcaaaag atttgttcgt cagtagttga cctgaacggc ggctcgctct atcttcttgc
    25921 agccctgcgt atattgcggc tcgcggatgc ggaccccttt cccctcttca cgcactcttg
    25981 caggtattga cccttgacgc cagggtaagc acatggcgtt tgttacgata gtggcatatg
    26041 aatttaaccc tcaaagaatc gcttgttacc cgtagccggg tatttagccc gtggactgcg
    26101 ttctactttt tacagtcgct attaattaac ctcggcttag gttacccctt cagtttgctc
    26161 tacaccgctg cgtttacggc tattttgctt ttgctatggc gaacattgcc tcgcgtacaa
    26221 aaagttctgg tcggtgtcag ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc
    26281 tacggcgcgc ctaatttcaa tacattgctg gcattgcact ccaccaatat ggaagagtcg
    26341 accgaaatcc tgacgatttt tccgtggtac agctacctgg tcggcttatt tatttttgcg
    26401 ctcggcgtaa tagcaatcag gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc
    26461 gacagcctgt gtctggtatt cagtgtggcg acattttttg ttgctcccgt gcaaaacctg
    26521 gcctggggtg gcgtatttaa actgaaagat actggctatc cggtatttcg ttttgctaag
    26581 gatgtcatcg tcaataataa cgaggtgatt gaagagcaag aacggatggc aaaactttcc
    26641 ggaatgaaag atacctggac ggtcactgcc gttaagccga agtatcagac ctatgtggtg
    26701 gtgatcggcg aaagcgcgcg tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat
    26761 accccgtttg ccagcagcgt taacggtttg atatttgctg actacattgc cgccagtggc
    26821 tccacgcaga aatcacttgg cttaacgctc aatcgcgtag tcgatggcaa accacagttt
    26881 caggataact ttgtcaccct ggcaaatcgc gcgggcttcc agacctggtg gttttccaac
    26941 cagggccaaa tcggcgaata cgatactgct atcgccagca tcgccaaacg tgcagatgaa
    27001 gtgtacttcc tgaaagaagg taattttgaa gcagataaaa acacgaaaga cgaagcgtta
    27061 ctggatatga ccgctcaagt gctggcgcaa gagcactcgc aaccgcagct gattgttctg
    27121 catctgatgg gctcgcatcc gcaggcctgc gacaggacac aaggcaaata cgaaaccttt
    27181 gtgcaatcga aagaaacgtc gtgctatctc tataccatga cgcaaacgga cgatttactg
    27241 cgcaagctgt acgatcagtt acgcaacagc ggcagcagct tctctctggt ttacttttct
    27301 gaccacggtc tggcctttaa agagcgcggc aaagacgtgc aataccttgc ccatgatgat
    27361 aaatatcagc aaaatttcca ggtgcctttt atggtcattt ccagcgacga taaagcgcat
    27421 cgggtgatta aagcccgccg ctcagccaat gacttcttag gctttttctc gcagtggacg
    27481 ggaattaaag cgaaggaaat aaatatcaaa tacccgttta tatctgagaa gaaagccggg
    27541 ccgatataca tcaccaactt ccagttacag aaggtggatt acaaccatct cggaaccgat
    27601 attttcgacc cgaaacctta aaacaaaaaa tccgccccga gaggcggatt ttttatatca
    27661 ccaaagtgat tagaagcggt aaccaacacc ggcaatccag gtgcctacgt caacgctacg
    27721 aatacggctc tgctcgtaag agaagtccag agcaacgttt tccatcgggt tgaactgcag
    27781 accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt
    27841 ggtctggaat ttaccataac ccacccccac tacaccgtag atgcttgccc agtcgttaat
    27901 gcggtaagcc ggaccagcag tgatgccgta gtactggttt ttgttgtagt caccagagct
    27961 tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc
    28021 ttcttcatag cggtatttca ggttgaaacc gcccattttg ttcatttggc cctgagcgtc
    28081 gctctgtgcg taaccgccag ttacggtaga agtcgcagct acggaagtac ctgcggtgaa
    28141 agccagaact gcggccagtg ctgaaagaca tgcaattttt ttcataacca cctcaaatgt
    28201 gattcaaata agttctaagt tttaaatata tcaaaaatta atgggaaact cttcgcgatt
    28261 tgtgatgtct aacgggccat ttcatgtaac agaacgtttc catacaccgc tatccatcta
    28321 aatttaaatc actttttcag agaactgcgt aagtattacg catgttttcc ctgtcattca
    28381 tccagattat tcctaatcac cagactaatg attccatcaa tcctggcgca ttttagtcaa
    28441 aacgggggaa aattttttca acaaatgctc aaccagcatt gggtatatcc agtacactcc
    28501 acgctttact taagtctaga tatttgtggg agaaaggatg cctggttcat tacgtaaaat
    28561 gccggtctgg ttaccaatag tcatattgct cgttgccatg gcgtctattc agggtggagc
    28621 ctcgttagct aagtcacttt ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct
    28681 ggcattagga acgctgatcc tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa
    28741 agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg ggtgggatga attatctttt
    28801 ttatctttct attcagacag taccgctggg tattgcggtg gcgctggagt tcaccggacc
    28861 actggcggtg gcgctgttct cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc
    28921 ggttcttggt ctgtggttcc tgctaccgct ggggcaagac gtttcccatg tcgatttaac
    28981 cggctgtgcg ctggcactgg gggccggggc ttgttgggct atttacattt taagtgggca
    29041 acgcgcagga gcggaacatg gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt
    29101 aattttcgtg ccaattggag cgcttcaggc tggtgaagca ctctggcact ggtcggttat
    29161 tccattgggt ctggctgtcg ctattctctc gaccgctctg ccttattcgc tggaaatgat
    29221 tgccctcacc cgtttgccaa cacggacatt tggtacgctg atgagcatgg aaccggcgct
    29281 ggctgccgtt tccgggatga ttttcctcgg agaaacactg acacccatac agctgctggc
    29341 gctcggcgct atcatcgccg cttcaatggg gtctacgctg acagtacgca aagagagcaa
    29401 aataaaagaa ttagacatta attaaattta catttctgca tggttatgca taaccatgca
    29461 gaatttctcg ctacttttcc tctacaccgt ctttatatat cgaattatgc aaaagcatat
    29521 ttattccgaa aattcctggc gagcagataa ataagaattg ttcttatcaa tatatctaac
    29581 tcattgaatc tttattagtt ttgtttttca cgcttgttac cactattagt gtgataggaa
    29641 cagccagaat agcggaacac atagccggtg ctatacttag tctcgttaat tactgggaca
    29701 taacatcaag aggatatgaa attatgagta ccgctaaatt agttaaatca aaagcgacca
    29761 atctgcttta tacccgcaac gatgtctccg acagcgagaa aaaagcaaca gtagagttgc
    29821 tgaatcgcca ggttatccag tttattgatc tttctttgat taccaaacaa gcgcactgga
    29881 acatgcgcgg cgctaacttc attgccgtac atgaaatgct ggatggcttc cgcaccgcac
    29941 tgatcgatca tctggatacc atggcagaac gtgcagtgca gctgggcggt gtagctctgg
    30001 ggaccactca agttatcaac agcaaaaccc cgctgaaaag ttacccgctg gacatccaca
    30061 acgttcagga tcacctgaaa gaactggctg accgttacgc aatcgtcgct aatgacgtac
    30121 gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga tatcctgacc gccgcgtctc
    30181 gcgacctgga taaattcctg tggtttatcg agtctaacat cgaataaatc catcgctgat
    30241 ggtgcagaac tttagtaccc gataaaagcg gcttcctgac aggaggccgt tttgttttgc
    30301 agcccacctc aacgcactta tttagtgcat ccatctgcta tctccagctg attaagtaaa
    30361 ttttttgtat ccacatcatc acacaatcgt tacataaaga ttgttttttc atcaggtttt
    30421 acgctaaata atcactgtgt tgagtgcaca attttagcgc accagattgg tgccccagaa
    30481 tggtgcatct tcagggtatt gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa
    30541 aaatcaagga gttgcaaaac tggcacgatt ttttcatata tgtgaatgtc acgcagggga
    30601 tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg tattaaaagt ttcactggct
    30661 gcactgaccc tggcttttgc ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg
    30721 acggataccg ccttcgttcc gtttgaattt aaacagggcg ataaatatgt gggctttgac
    30781 gttgatctgt gggctgccat cgctaaagag ctgaagctgg attacgaact gaagccgatg
    30841 gatttcagtg ggatcattcc ggcactgcaa accaaaaacg tcgatctggc gctggcgggc
    30901 attaccatca ccgacgagcg taaaaaagcg atcgatttct ctgacggcta ctacaaaagc
    30961 ggcctgttag tgatggtgaa agctaacaat aacgatgtga aaagcgtgaa agatctcgac
    31021 gggaaagtgg ttgctgttaa gagcggtact ggctccgttg attacgcgaa agcaaacatc
    31081 aaaactaaag atctgcgtca gttcccgaac atcgataacg cctatatgga actgggcacc
    31141 aaccgcgcag acgccgttct gcacgatacg ccaaacattc tgtacttcat caaaaccgcc
    31201 ggtaacggtc agttcaaagc ggtaggtgac tctctggaag cgcagcaata cggtattgcg
    31261 ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc
    31321 gagaacggaa cttacaacga aatctacaaa aaatggttcg gtactgaacc gaaataataa
    31381 cgctacacct gtaaagcgca ctggcagttc cctctcccct ctggggagag gattagggtg
    31441 aggggcgcaa acccgctccg gggccattaa ttaccctgaa tttgattatt tacaccacgg
    31501 taacaggaac aacatatgca gtttgactgg agtgccatct ggcctgccat tccgcttctg
    31561 attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg gtctggctgg cggtctggta
    31621 atcggattgc tggcaggttt tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg
    31681 ctggtcttta ttgaggtgat ccgcggcaca cctatcgtcg tccaggtgat gtttatttat
    31741 ttcgccctgc cgatggcgtt taacgactta cgcatcgacc catttactgc ggcggtggtc
    31801 accatcatga tcaactccgg cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct
    31861 attcacaaag gttttcgtga agcaggactg gcgctcggtc tttcacgttg ggaaaccatt
    31921 cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg
    31981 atcatcagca ttaaagacac ctcgctgttt attgtgatcg gcgtggcgga actgacccgt
    32041 caggggcaag aaattattgc cggtaacttc cgcgcccttg agatctggag cgccgtggcg
    32101 gtgttctatc tgattattac cctggtgcta agctttattt tgcgtcgtct ggaaagaagg
    32161 atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca ctttggccca acccaggtgc
    32221 ttcacaatat cgatttgaac attgcccagg gcgaagtcgt ggtgattatc gggccgtccg
    32281 gttccggtaa atcgaccctg ctgcgctgca tcaacaaact ggaagaaatc acctccggcg
    32341 atctgattgt cgatggcctg aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc
    32401 aggaagcagg tatggtgttc cagcagtttt acctcttccc gcatctgacg gcgctggaaa
    32461 acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg
    32521 cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc acatcactac ccttccgaac
    32581 tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa
    32641 tgatgctgtt tgatgaaccg acttccgctc ttgacccgga actgcgccat gaagtgctga
    32701 aggttatgca ggatctggct gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg
    32761 gttttgccga gaaagtagct tcgcggctga tctttatcga caaaggccgg attgcggaag
    32821 atggcgatcc gcaggtattg atcaagaacc cgccgagcca gcgcttgcag gaatttttgc
    32881 agcacgtctc ttaacttctc tgcctccctt tcacccgaaa gggaggcaca ccagattcct
    32941 ctcatttaaa atcgcccctc ccccagcatc tatacttatc tttttgctct gttttctcac
    33001 tggaggagtc atgcggtgga tcctgttcat cctcttctgc ctgctgggcg cacctgccca
    33061 cgcggtatcc atacccggcg ttacaaccac aacgacaacg gactcaacga ctgaaccggc
    33121 cccggaaccg gatatcgaac aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga
    33181 taacgacacc tcgcgtaaag aattgatcga ccagttgcgt actgtcgccg cgacgccccc
    33241 tgcggaaccg gtcccgaaga tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca
    33301 aaaggtcacc gaagtcagcc gccattatgg tgaagccctt tccgcccgct tcgggcaact
    33361 ttatcgcaat atcaccggct ccccgcataa gccatttaat ccacaaacct tcagcaatgc
    33421 gctgagccat ttttcaatgt tagcggtatt agtgtttggt ttttactggc tgattcgcct
    33481 gtgcgcactg ccgctgtatc gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg
    33541 cagcaactgg ttgcagcttc cggcgatgat tatcggggcg tttattatcg acctgctgtt
    33601 actggccctg acattatttg tcggccaggt attaagcgac aacctgaatg caggcagtcg
    33661 caccatcgct ttccaacaaa gtttgtttct caacgccttt gccctgattg aatttttcaa
    33721 agccgtacta cgcctgattt tttgcccaaa cgtggcggag ctgcgcccgt tcacgattca
    33781 ggacgagagc gcccgttact ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta
    33841 tggcctgatt gtggccgtgc cgattatctc taatcaggtg aatgtacaga taggtgcgct
    33901 ggcgaacgtc atcattatgc tgtgcatgac cgtctgggcg ttgtacctga tctttcgtaa
    33961 taaaaaagag attacccagc atttgctcaa ctttgcggag cattcgctgg cctttttcag
    34021 cctgtttatc cgcgcctttg cgctggtgtg gcactggctg gcaagcgcct attttatcgt
    34081 gctgtttttc ttttcgttgt tcgatccggg caacagcctg aaatttatga tgggtgcaac
    34141 ggtgcgcagc ctggcgatta ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg
    34201 gctggcaaaa accatcactc tctcgccaca tactcagcgt aactatccag agttgcaaaa
    34261 acggctgaat ggctggcttt cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt
    34321 ggcggtaatg ttgctgttga gcgcatgggg attgttcgat ttctggaact ggctgcaaaa
    34381 cggcgcgggg cagaaaaccg tagatatcct gatccgtatc gcacttattc tttttttctc
    34441 ggcggttggc tggacggtgc tcgccagttt gatcgaaaac cggctggctt cggatattca
    34501 tggccgcccg ctacccagcg cccgtacgcg taccctgctg acgctgttcc gtaacgcgct
    34561 ggcggtgatt atcagtacca tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat
    34621 cgcgccattg ctggcaggtg ccggggcatt aggtctggct atctcgtttg gttcgcaaac
    34681 gctggtgaaa gatatcatta ccggggtgtt tattcagttt gaaaacggca tgaacactgg
    34741 agatttggtg actatcgggc cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt
    34801 gggcgtgcgc caggataccg gggcgtatca catcattccg tggtcttcga taaccacctt
    34861 tgctaacttc gtccgcggta ttggttcggt agtggcaaac tacgatgttg atcgccatga
    34921 agatgctgat aaagccaatc aggcactgaa agatgcggta gcggaattaa tggaaaacga
    34981 agaaattcgc gggctgatta ttggtgaacc gaattttgcc gggattgtcg gcttaagcaa
    35041 taccgcgttt acactgcgtg tttcgttcac cacgctgcca ctcaaacagt ggacggtacg
    35101 ctttgccctc gacagccagg tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt
    35161 gcagacttat caggtgctgc ctactccggg cgcgaccccg gctgaaccgt taccgccggg
    35221 ggagccaacg ctttaacgct ggcgattgac aaaacggcgg cgctgctcgt cgttcataaa
    35281 ggtccaggca ataaagcgac tctgcttttg cccctgggcc atctcttttt ttaccacctt
    35341 caccgcgccc acgtcggtca gagcacgata caacggcggt aagttttcac cacgagatac
    35401 cagcgaggta aaccacatca cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt
    35461 gataaaggcg acttcaccac cttcacacca caactcctgt tgctgaccac caaagttcag
    35521 tgcatcgtct ttgttcagcc ccaggttacg gcgtttacgc tcactacctg cccgcgctgc
    35581 agcggcggaa tcgtggaatg gcgggttaca caaggtcgcg tcgtattgct cgtttttatg
    35641 gatgatgccg ttaaaaattg ccccactctc tttttgccga cgcagacgaa tggcgcggtt
    35701 aagccccgga ttagcactga taatcgcctg cgcactgctt aacgcctggc tgctggtttc
    35761 actaccggta aaacgccagc catattcatg tacgccaatc agcggataaa tacagttcgc
    35821 accaacgccg atatccagaa tgctggcatt cgctggaatt gttccgcttg cttcagccag
    35881 taaatcggca aggtgatgaa tataatccgc ccggcccggt actggtgggc agagaaaacc
    35941 gtcggggata tcccagttcg ctacggcgta aaaatgggcc agcaacgcct tattgagcgc
    36001 cttcaccgcc agcggattgg caaagtctac gctttgctcc ccggcgggtg taagcgtgag
    36061 gaattgcctg agttcaggat tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg
    36121 acggttgcgc ggatgcaacc ccggtttctg ggcggacata gcattctcct ttaagcagcg
    36181 gcgtaagata cccgcataac gcgacagggt aaatatctgg ctgcgtttat cattccccaa
    36241 aaccttaaca tggctatggt ttatggcaat tcatcataaa aatcagcatt atgcatgtca
    36301 ttaatttgtc ttattttgta aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt
    36361 ttttgcgtca tttgcatcgc gcaggcgacc tacacttatc aggcactacc cgttgtattt
    36421 atgaggatgt cattatgaaa aagtgcctca cactactgat tgccaccgtc ctgagcggaa
    36481 tctctctcac ggcttatgcc gcgcaaccga tgagtaacct ggacagcggt cagctacggc
    36541 ccgccggtac cgtttcggca actggcgcat caaacctaag cgatctggag gataaactgg
    36601 cagaaaaagc gcgcgaacaa ggcgcgaaag gttatgtcat taattccgca ggcggaaatg
    36661 accagatgtt cggtactgca accatctaca aataacccca ccaacgtaac tgcattcgcc
    36721 tctggatgca gttttcgcct gttttgtaat caaaaaacat caaatatgaa ctcaatgtaa
    36781 ataaatgtat ttctttttcg cgcaatgggt gatagaaaat cgcgccaagt gataatgctt
    36841 atcaaaatta ttatcacttg cacgaacact atcacgggat taacagtggc atcgcatccg
    36901 cagagatgct ttctcgtggc agtgaaaatt tcaacatata agaaaaagtc acctgcaaaa
    36961 tggaaaacaa tcgcaatttc cctgccagac aatttcattc gctcacgttc tttgccggtc
    37021 tttgtattgg catcacgcct gtggctcagg cactcgccgc cgaagggcaa actaacgcgg
    37081 atgacacgct ggttgtcgaa gcatcaacgc cttcgcttta tgcgccacaa caatctgccg
    37141 atccgaaatt ctcgcgtccg gtagcggata ctacccgcac gatgacggtg atttctgaac
    37201 aagtgattaa agatcagggc gcaaccaacc ttaccgacgc gctcaaaaac gcccccggcg
//
LOCUS       DAZOZY010000020        36796 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZOZY010000020 DAZOZY010000000
VERSION     DAZOZY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.020"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHURQ_000876"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHURQ_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0822725.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHURQ_000877"
     CDS             complement(280..873)
                     /locus_tag="ACHURQ_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0822726.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHURQ_000878"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHURQ_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0822727.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHURQ_000879"
     CDS             1788..2183
                     /locus_tag="ACHURQ_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0822728.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHURQ_000880"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHURQ_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR0822729.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHURQ_000881"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHURQ_000881"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR0822730.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHURQ_000882"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHURQ_000882"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR0822731.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHURQ_000883"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHURQ_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR0822732.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHURQ_000884"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHURQ_000884"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR0822733.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHURQ_000885"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHURQ_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR0822734.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHURQ_000886"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHURQ_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR0822735.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHURQ_000887"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHURQ_000887"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR0822736.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHURQ_000888"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHURQ_000888"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR0822737.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHURQ_000889"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHURQ_000889"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR0822738.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGCEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHURQ_000890"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHURQ_000890"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR0822739.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHURQ_000891"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHURQ_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR0822740.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHURQ_000892"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHURQ_000892"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR0822741.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHURQ_000893"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHURQ_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR0822742.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHURQ_000894"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHURQ_000894"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR0822743.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHURQ_000895"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHURQ_000895"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR0822744.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHURQ_000896"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHURQ_000896"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR0822745.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHURQ_000897"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHURQ_000897"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR0822746.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHURQ_000898"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHURQ_000898"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR0822747.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVDYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHURQ_000899"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHURQ_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR0822748.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHURQ_000900"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHURQ_000900"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR0822749.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHURQ_000901"
     CDS             complement(26207..26548)
                     /locus_tag="ACHURQ_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR0822750.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHURQ_000902"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHURQ_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR0822751.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHURQ_000903"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHURQ_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR0822752.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHURQ_000904"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHURQ_000904"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR0822753.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHURQ_000905"
     CDS             33227..34570
                     /locus_tag="ACHURQ_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR0822754.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34667..34873)
                     /locus_tag="ACHURQ_000906"
     CDS             complement(34667..34873)
                     /locus_tag="ACHURQ_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR0822755.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35198..35752
                     /locus_tag="ACHURQ_000907"
     CDS             35198..35752
                     /locus_tag="ACHURQ_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR0822756.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35842..36753)
                     /gene="ytfI"
                     /locus_tag="ACHURQ_000908"
     CDS             complement(35842..36753)
                     /gene="ytfI"
                     /locus_tag="ACHURQ_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR0822757.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
BASE COUNT         8913 a         9853 c         9141 g         8889 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtattgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggctgcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agtccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcacttttta ctgcatagca ctttggttaa gccatctctg
    34681 tttgcagacg cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct
    34741 tcacttttgg gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt
    34801 ataaccgtcc acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt
    34861 ggaaaatttt cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc
    34921 caactattat gaggcgcaaa ttttaatgct tagtgactat agactatccg ggcaatgttt
    34981 ccaccgcgta taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg
    35041 aagcgcggtc ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac
    35101 tggcatcaca cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag
    35161 tggtcggatc acctgcatat cataagaagg aaacaccatg accctacgca agattctggc
    35221 actcacctgc ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg
    35281 agtgccgccg attggcatta ccgatcgggg cgagttggtg cttgataaag atcagtttag
    35341 ctacaaaacc tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc
    35401 tggtcgcacc tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa
    35461 gttaccgcac gatcgttacc agaccaccac tattgttaac accgacgacg cgattccggg
    35521 ttcagggatg tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca
    35581 gtttattgtc gatagcaatg gcgtcgcgcg cggtgcctgg cagctggatg aagaaagttc
    35641 cgctgtagtg gtgctggata aggatggtcg cgtgcaatgg gccaaagacg gggcgctcac
    35701 tcaggaagag gtgcagcaag tgatggacct gctgcaaaag ttgctcaaat aatggcaaca
    35761 acatagattc tgcaaaaata attttcccct atcgttatct atgggatagg ggaattaagt
    35821 ctctgagttc ttacgccgaa ctcatactgg taacgctagt tgctcttttt taaacgcctc
    35881 aatggtcgac ttatatttct cgcaaatctt ttctttggta atattactat ctgcggccag
    35941 attcgcactt actatcgcat ccacttgttc ggctaattcc acttgttggc aacctgcatt
    36001 aaccagaaaa ttataaataa gggagtaatc ttcatcctcc aaaacaacag aggaaccatt
    36061 acgaaaacgt tcatcccctc catagatttt aagcgcaaag cccaaagatt ctatgactat
    36121 ggcaaaatta tcatcaatta ttttttcgtg aatataccat ccatcgcatt ctttaattcc
    36181 acctcctggt atttggagtt caaatttacc ccgttttaat ttgcttacta tatcctccaa
    36241 taaggcataa cctaaagaca cgtcaccaat cgcctggtga atataacctt taatcctttt
    36301 cggtatgtca gactgttttt tctgcagaat atcacaggca tacaatcttc tgttttgttc
    36361 taacggaaac tcgcggtttt tgaaatccat gaatgagtta tactcattta ttgttatggg
    36421 aatttcttcg tattcaaatc cactacctcg caatgtcatt gtatattcgg caccgttatc
    36481 tttaaggctg aaactgataa ggaacttatc catggcgtta acagttccat tttttgatga
    36541 aagtacctga taactatttt tatcaatctg agttgcgcaa cgaaagaact ccctcaaagt
    36601 taacaggcta tcataattcg aaatccaatc ttttttctta aatgcatgct ggatattgtt
    36661 aaaaaaattg tttgaatgtg tttttgtata cgatgacttt acaaataact ccaccgcacc
    36721 aataaaatta ttgtgtctga tcctgggtaa catatgacaa ccttatttta tttggttatt
    36781 ttatgtcgcc attaaa
//
LOCUS       DAZOZY010000021        34981 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZOZY010000021 DAZOZY010000000
VERSION     DAZOZY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34981
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.021"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..815)
                     /locus_tag="ACHURQ_000909"
     CDS             complement(<1..815)
                     /locus_tag="ACHURQ_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0822758.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACHURQ_000910"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACHURQ_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACHURQ_000911"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACHURQ_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1397..1873)
                     /locus_tag="ACHURQ_000912"
     CDS             complement(1397..1873)
                     /locus_tag="ACHURQ_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR0822759.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHURQ_000913"
     CDS             complement(1932..3221)
                     /gene="bioA"
                     /locus_tag="ACHURQ_000913"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR0822760.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3308..4348
                     /gene="bioB"
                     /locus_tag="ACHURQ_000914"
     CDS             3308..4348
                     /gene="bioB"
                     /locus_tag="ACHURQ_000914"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR0822761.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            4345..5499
                     /gene="bioF"
                     /locus_tag="ACHURQ_000915"
     CDS             4345..5499
                     /gene="bioF"
                     /locus_tag="ACHURQ_000915"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR0822762.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5486..6241
                     /gene="bioC"
                     /locus_tag="ACHURQ_000916"
     CDS             5486..6241
                     /gene="bioC"
                     /locus_tag="ACHURQ_000916"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR0822763.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGALPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6234..6902
                     /gene="bioD"
                     /locus_tag="ACHURQ_000917"
     CDS             6234..6902
                     /gene="bioD"
                     /locus_tag="ACHURQ_000917"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0822764.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHURQ_000918"
     CDS             7481..9502
                     /gene="uvrB"
                     /locus_tag="ACHURQ_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR0822765.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHURQ_000919"
     CDS             complement(9694..10602)
                     /gene="yvcK"
                     /locus_tag="ACHURQ_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR0822766.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10867..11011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10999..11988
                     /gene="moaA"
                     /locus_tag="ACHURQ_000920"
     CDS             10999..11988
                     /gene="moaA"
                     /locus_tag="ACHURQ_000920"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR0822767.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12010..12522
                     /gene="moaB"
                     /locus_tag="ACHURQ_000921"
     CDS             12010..12522
                     /gene="moaB"
                     /locus_tag="ACHURQ_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR0822768.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12525..13010
                     /gene="moaC"
                     /locus_tag="ACHURQ_000922"
     CDS             12525..13010
                     /gene="moaC"
                     /locus_tag="ACHURQ_000922"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR0822769.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13003..13248
                     /gene="moaD"
                     /locus_tag="ACHURQ_000923"
     CDS             13003..13248
                     /gene="moaD"
                     /locus_tag="ACHURQ_000923"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR0822770.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13250..13702
                     /gene="moaE"
                     /locus_tag="ACHURQ_000924"
     CDS             13250..13702
                     /gene="moaE"
                     /locus_tag="ACHURQ_000924"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR0822771.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            13839..14543
                     /locus_tag="ACHURQ_000925"
     CDS             13839..14543
                     /locus_tag="ACHURQ_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0822772.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14814..15461
                     /locus_tag="ACHURQ_000926"
     CDS             14814..15461
                     /locus_tag="ACHURQ_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR0822773.1"
                     /translation="MWLITALGLVATAGLAWGAKYLEITATKYDSPPMYVAIGLLLLC
                     MYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVPVYAIIIVFSTAGAMFLISMLAG
                     LLFNVEPGSHRFIIMMTLTGLALVIIVNAALMSERPIWVISCLMIVLWPGIISHGRNK
                     LLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFGILAAIAITLVWQRHTCFFH"
     gene            complement(15526..16237)
                     /locus_tag="ACHURQ_000927"
                     /pseudo
     CDS             complement(15526..16237)
                     /locus_tag="ACHURQ_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHURQ_000928"
     CDS             complement(16237..17478)
                     /gene="clsB"
                     /locus_tag="ACHURQ_000928"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR0822774.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTRWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17475..18236)
                     /locus_tag="ACHURQ_000929"
     CDS             complement(17475..18236)
                     /locus_tag="ACHURQ_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0822775.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18369..18779
                     /locus_tag="ACHURQ_000930"
     CDS             18369..18779
                     /locus_tag="ACHURQ_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR0822776.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18741..19847)
                     /locus_tag="ACHURQ_000931"
     CDS             complement(18741..19847)
                     /locus_tag="ACHURQ_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0822777.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19858..21000)
                     /locus_tag="ACHURQ_000932"
     CDS             complement(19858..21000)
                     /locus_tag="ACHURQ_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0822778.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20993..22729)
                     /locus_tag="ACHURQ_000933"
     CDS             complement(20993..22729)
                     /locus_tag="ACHURQ_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0822779.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHURQ_000934"
     CDS             complement(22722..23717)
                     /gene="hlyD"
                     /locus_tag="ACHURQ_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR0822780.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHURQ_000935"
     CDS             complement(23720..24391)
                     /gene="cecR"
                     /locus_tag="ACHURQ_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR0822781.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHURQ_000936"
     CDS             24620..25984
                     /gene="rhlE"
                     /locus_tag="ACHURQ_000936"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR0822782.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHURQ_000937"
     CDS             complement(26216..26698)
                     /gene="ybiA"
                     /locus_tag="ACHURQ_000937"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR0822783.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26818..28968
                     /gene="dinG"
                     /locus_tag="ACHURQ_000938"
     CDS             26818..28968
                     /gene="dinG"
                     /locus_tag="ACHURQ_000938"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR0822784.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHURQ_000939"
     CDS             28996..29958
                     /gene="ybiB"
                     /locus_tag="ACHURQ_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR0822785.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHURQ_000940"
     CDS             30099..31184
                     /gene="hcxB"
                     /locus_tag="ACHURQ_000940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR0822786.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHURQ_000941"
     CDS             31251..31310
                     /gene="ybiE"
                     /locus_tag="ACHURQ_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR0822787.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHURQ_000942"
     CDS             complement(31413..31673)
                     /gene="ybiJ"
                     /locus_tag="ACHURQ_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR0822788.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHURQ_000943"
     CDS             complement(31938..32204)
                     /gene="ybiI"
                     /locus_tag="ACHURQ_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR0822789.1"
                     /translation="MASGWANDDAVNEQINSTIENAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHURQ_000944"
     CDS             complement(32278..32955)
                     /gene="ybiX"
                     /locus_tag="ACHURQ_000944"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR0822790.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32997..34981)
                     /locus_tag="ACHURQ_000945"
                     /pseudo
     CDS             complement(32997..34981)
                     /locus_tag="ACHURQ_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8269 a         8976 c         9491 g         8245 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcgtt cagcttttta tactaacttg agcgaaacgg
     1321 gaaggtaaaa agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg
     1381 ccatctggca gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga
     1441 aatgaacgtt aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta
     1501 tatccagcgc gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc
     1561 catcgtaccc ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta
     1621 ccagaccaga gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta
     1681 cccagtgcca ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac
     1741 ttttcgttcc cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt
     1801 aacccatgcc gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac
     1861 tgatgagttt catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg
     1921 cggacttctc gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg
     1981 gtcagacgct gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg
     2041 ccaaaaggcc ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc
     2101 accggatgag tggtttcgac cacgccaatg gcccccagta cgcgcacatc ggcaaccatt
     2161 tcggcatcac gggcgggggc aagttgctcg cgcagctgta cttcaatatc cgccacctgt
     2221 tgctgccagt cgccagattc gagaatcgcc aggctggcgt ttgctgccgc gcaggccagc
     2281 ggattgccca taaaagttgg cccatgcata aagcaaccgg cttcaccgtt actgatggtt
     2341 tctgcaacct cgcgcgtggt gagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct
     2401 ttaccgaggc acaaaatgtc cggcgcgatt tctgcatgtt cacaggcaaa cagtttcccg
     2461 gtacgaccaa atccagtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcat
     2521 attttgcgga ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg
     2581 acaatcggct caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca
     2641 aagcccacca tatcgcgctc atcccattcg ccatccatac ggctttgtgg ggcgggagca
     2701 aacaagtttt ctggcagata gcctttccac aggctgtgca ttgagttgtc cggatcgcac
     2761 accgacatcg cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg
     2821 cgcgcttcgc ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct
     2881 acggaaccgg agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt
     2941 ttgcggcaca gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg
     3001 gcatcaattt gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc
     3061 gcccaccagg acgacatacc gtcaaccagg cgtctgccgt cagacaaaat cagctcgcaa
     3121 ccttcggcgc tcaccaccgg ataaaccggc agcggggagg tcatggatgt gtatgggtgc
     3181 cagatatggc gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa
     3241 ttgaaaagat ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag
     3301 aagccccatg gctcaccgcc cacgctggac cttgtcgcaa gtcacagaat tatttgaaaa
     3361 accgttgctg gatctgctgt ttgaagcgca gcaggtccat cgccagcatt tcgatcctcg
     3421 tcaggtccag gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa
     3481 atactgcccg caaagctcac gctacaaaac cgggctggaa gccgagcggt tgatggaagt
     3541 tgaacaggtg ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat
     3601 gggcgcggcg tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca
     3661 gggggtaaaa gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca
     3721 ggcgcagcgc ctcgcgaacg ccgggctgga ttattacaac cacaacctgg acacctcgcc
     3781 ggagttttac ggcaatatca tcaccacccg cacttatcag gaacgcctcg atacgctgga
     3841 aaaagtgcgc gatgccggga tcaaagtctg ttctggcggc attgttggct taggcgaaac
     3901 ggtaaaagat cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag
     3961 cgtaccaatc aacatgctgg tgaaggtgaa aggcacgcca ctggcggata acgatgatgt
     4021 cgatgccttt gattttattc gcactattgc ggtagcgcgg atcatgatgc cgacctctta
     4081 cgtgcgcctt tctgccggac gcgagcagat gaatgaacag actcaggcga tgtgctttat
     4141 ggctggcgca aactcgattt tctacggttg caaactgctg accacgccga atccggaaga
     4201 agataaagac ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct
     4261 ggcaggggat aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga
     4321 cgaatattac aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat
     4381 gcacggcgta ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc
     4441 tggctggtgg cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta
     4501 agccatcatc cgcaaattat ccgcgcctgg cagcagggcg cagaaaagtt tggtgtaggt
     4561 agcggcggtt ccgggcatgt cagcggttat agcgtggtgc atcaggcgct ggaagaagaa
     4621 ctggccgagt ggcttggcta ttcgcgggcg ctgctgttta tctctggttt tgccgcgaat
     4681 caggcagtca ttgccgcgat gatggcgaaa gaggaccgta ttgttgccga ccggcttagc
     4741 catgcctcct tgctggaggc tgccagttta agcccgtcgc agcttcgccg ttttactcat
     4801 aacgatgtcg ctcatctggc gcgactgctt gcttccccct gtccggggca gcaactggtg
     4861 gtgacagaag gcgtgttcag catggacggc gatagcgcgc cactggcgga aatccagcag
     4921 gtaacgcaac agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc
     4981 ggggagcagg ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtggtg
     5041 acttttggca aaggatttgg cgtcagtggg gctgcggtgc tttgctccag tacggtggcg
     5101 gattatctgc tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag
     5161 gcgcaggcat tacgtgcgtc gctggcggtc attcgccgtg atgaggggga tgcacggcgc
     5221 gaaaaactgg tgtcactcat tgcgcgtttt cgtgccggag tacaggattt gccgtttacg
     5281 cttgctgatt catgcagcgc catccagcca ttgatcgtcg gtgataacag ccgtgcgtta
     5341 caactggcag aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc
     5401 gtacccgctg gtactgcgcg actgcgctta acgctaaccg cagcgcatga aatgcaggat
     5461 atcgaccgtc tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc
     5521 atttggtcgg gcagccgcgc actatgagca acacgcagag ttacagcgcc ggagtgctga
     5581 cgtcttactg gcaatgctcc cacagcgtaa atacacccac gtactggacg cggggtgtgg
     5641 gccaggctgg atgagccgcc actggcgaga gcgtcacgca caggtgacgg cgttagatct
     5701 ctcaccgcca atgcttgtgc aagcccgcca gaaagatgct gcagaccatt atctggcggg
     5761 agatatcgaa tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc
     5821 ggtgcagtgg tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc
     5881 cggaggcgtg gtggcgttta ccacgctggt gcagggagcg ttacccgaac tgcatcaggc
     5941 gtggcaggcg gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga
     6001 acagtcgctg aacggcgtgc attatcaaca tcatattcag cccatcacac tatggtttga
     6061 tgatgcgctc agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg
     6121 gcgcgacccg cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca
     6181 acaacagggg cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta
     6241 aacgttattt tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac
     6301 ttttacaagc cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg
     6361 gcagcgaaaa gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca
     6421 gcagcctgca gctggattac gcaacagtaa atccttacac cttcgcagaa cccacttcgc
     6481 cgcacatcat cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat
     6541 tacgcgcgct tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta
     6601 cgccgctttc tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga
     6661 tactggtagt tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa
     6721 tacaacacgc cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa
     6781 aacgtcacgc tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag
     6841 agatcccctg gcttgcagaa aatgcggcaa ccggaaagta cataaacctt gccttgttgt
     6901 agccattctg tatttggtta aattgcgagc gagatcgcgt cttcgattga ctgcaattta
     6961 accaattaaa ttctaaaata atcacgaaaa aaattttact tccgcctcat gcggcgaatg
     7021 tgggaattgc ccaggcggcg ggggataggg gctggagaca gttatccact attcctgtgg
     7081 ataaccatgt gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac
     7141 gcgaattggc gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa
     7201 atgcaatggc agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga
     7261 caaatgttaa aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc
     7321 cacagagtaa attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg
     7381 gtgatgaact gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa
     7441 attacatacc tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact
     7501 gaattccgct tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg
     7561 gctggaagat ggcctggcgc accagacgtt acttggcgtg actggctcag ggaaaacctt
     7621 caccattgcc aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa
     7681 aacgctggcg gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga
     7741 gtatttcgtc tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac
     7801 tttcattgag aaagatgcct cggttaacga acatattgag cagatgcgtt tatccgccac
     7861 caaagcgatg ctggagcggc gtgatgtggt tgtagtggcc tctgtttccg cgatttatgg
     7921 tctgggcgat cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat
     7981 cgatcagcgc gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc
     8041 attccagcgt ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc
     8101 ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt
     8161 tgacccgctg accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac
     8221 gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact
     8281 ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac
     8341 ccagcgtacc cagtttgatc tggagatgat gaacgagctg ggctactgtt cggggattga
     8401 aaactactcg cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga
     8461 ttacctgcct gccgatgggc tgctggtggt cgatgaatct cacgtcacca ttccacaaat
     8521 tggcggcatg tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg
     8581 cctgccatca gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc
     8641 gcaaaccatc tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga
     8701 tgtggtggat caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc
     8761 ggtggcgaca caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga
     8821 acgcgtactg gtcaccacac tgaccaagcg gatggcggaa gatcttaccg aatatctcga
     8881 agaacatggc gagcgcgtgc gttatcttca ttcagatatc gacaccgtcg aacgtatgga
     8941 gattatccgc gacttgcgtc tgggtgagtt cgacgtgctg gtagggatca acttactgcg
     9001 cgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg
     9061 cttcctgcgt tccgaacgtt cgttgatcca gactattggt cgtgcggcac gtaacgttaa
     9121 cggtaaagcg attctctacg gcgataagat caccccatca atggcgaaag cgattggcga
     9181 aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag gaacacggaa ttacgccgca
     9241 aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa
     9301 agcgaagggc agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat
     9361 gtcgcctaaa gcgttgcaac agaaaatcca tgagctggaa gggttaatga tgcaacacgc
     9421 gcagaatctg gagttcgaag aagcggcaca aattcgtgac cagttgcatc agctgcgtga
     9481 gctgtttatc gcggcttcgt gacaggataa cgaagaagac agatgacaaa agcaaaattg
     9541 cctgatgtgc tacgcttatc aggcctacat gatctctgca atacattgaa ttagcgcggt
     9601 ttgtaggccg gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc
     9661 aataggaagc cggaattttt cttcggattt ccgttaacct aaagcctgta acgccttttc
     9721 cagcgcgtta tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac
     9781 ctcctgaatc acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac
     9841 cgcatcaatg acctttttac caacatactg ctccataatt gccagcttgc tttccagctt
     9901 caaattagcc gcaggtaaac tcaactcacg ccccagattg ccgatataga ccatcggcgc
     9961 tggcgtgcgg cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt
    10021 ataaaaactg ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc
    10081 acgcgttgcg ggtacattcg gcgttaataa taactcttga atcggcgtag ttaactggtc
    10141 gatattgacc tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcgacggg
    10201 atgctctgac attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat
    10261 ggcttccaga ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt
    10321 atgaccggaa agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgct
    10381 cggttccgtt atcagttggt tgaggcagtt gcgcatatcg ccccaggcaa tgccgccttc
    10441 tgaacggcga atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa
    10501 acgagaaccc aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag
    10561 agcaacgaca cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag
    10621 attatccgcg ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga
    10681 ggatacaaac tattttcttc tacctctaaa ggacgatgca cgctatgcct ccctgatgat
    10741 gtatatcaaa gtaaaactgc cattttccct tattctgtag cgaaatagca cgatcatgac
    10801 gctatataca tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt
    10861 atcggcttaa ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg
    10921 gccgtgacaa tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc
    10981 gtatctggaa aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac
    11041 tacttgcgcc tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat
    11101 ggctacaaac cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg
    11161 gttacgcgcg ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg
    11221 tctttacgcc gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc
    11281 cagattgcgg tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat
    11341 gcgggactta ctggcattaa cgtcagtgtc gacagtctgg acgcccgcca gtttcacgct
    11401 attaccgggc aggataaatt caaccaggtc atggcaggga tcgatgctgc atttgaggtc
    11461 ggttttgaga aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc
    11521 gacacctttc tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg
    11581 gaaacgggcg agggcagtga gctcttccgt aagcatcaca tctctggtca ggttctgcgt
    11641 gacgagctac tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcccgcg
    11701 caagtctttt gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa
    11761 gacttctgcg ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc
    11821 ctgtttggtg aaggcggcgt taacctgcgc gatctgctgg aagacgatac ccagcaacag
    11881 gcgctggaag cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat
    11941 caaaacaaca ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg
    12001 agagatcaga tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg
    12061 gtttctaatc gtcgcggtga agaagacgat acctccggtc actatttgcg cgattcggcg
    12121 caagaagcgg gccatcacgt tgtcgataaa gccattgtga aagaaaaccg ctacgctatt
    12181 cgcgctcagg tatctgcgtg gatcgccagc gacgatgtac aagtggtgtt gattacgggg
    12241 gggactggcc tgacggaagg tgatcaggct cccgaagcat tgctgccgtt gttcgaccgt
    12301 gaagttgaag gttttggtga agtgttccgg atgttgtcgt ttgaagagat tggcacttcc
    12361 acgttgcaat ctcgtgcggt agcgggcgtc gccaacaaaa cgctgatttt cgccatgccg
    12421 ggttcgacca aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc
    12481 cgtacgcgtc cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc
    12541 atatcaacgc cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc
    12601 gtgaagcgcg ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg
    12661 atggtcgcca ccacaaaggc gacgtatttg ccactgcgcg tattgtcggt attcaggcgg
    12721 caaaacgcac ctgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag
    12781 tcaatttaca ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga
    12841 ccgggaaaac cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca
    12901 tttatgacat gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg
    12961 cgaagagcgg cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt
    13021 ttcgcccagg tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca
    13081 accgttgaag cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg
    13141 gaagatggca aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg
    13201 actgacggcg acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac
    13261 caaaattgtt gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggctga
    13321 gcgtgacgaa gacggtgcag tggtcacgtt taccggtaag gtgcgcaacc ataacctggg
    13381 tgacagcgtc aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc
    13441 agaaatcgtt gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg
    13501 catcggggaa ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg
    13561 cagcagtgcg tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt
    13621 ctggaagcgc gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatca
    13681 gcaggcggca aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca
    13741 atttcaccgc tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag
    13801 tatccttaag atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg
    13861 attcaatcgt acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga
    13921 tgaccgttgg cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg
    13981 tgatggagct gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag
    14041 cattggttat tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc
    14101 tctttatgct ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata
    14161 ccgctgcttc tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc
    14221 tgtacggtta caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt
    14281 taatcggcat tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt
    14341 gggcagttac ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga
    14401 aattgaaaaa tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt
    14461 ccattcttgg cgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac
    14521 ggatcttcgg caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg
    14581 atttttttct tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag
    14641 cgagcggaat cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa
    14701 tttattgatg gtgaatatta atattttttc taaggacaac taaaagaatg gaatcatact
    14761 cgcaaaacag caataaatta gattttcagc acgaggccag gatataaaac ggtatatggc
    14821 tcattaccgc tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg
    14881 aaattacggc aaccaaatat gattcaccac caatgtatgt cgctataggg ttattattac
    14941 tttgtatgta tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa
    15001 tatatctgtt tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg
    15061 ccattatcat cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt
    15121 tattatttaa tgttgagcct ggttctcacc gttttatcat tatgatgacg ttgacagggt
    15181 tggccctggt aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa
    15241 gttgcttaat gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg
    15301 aactggcagg aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag
    15361 gtgcattaac actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta
    15421 taacgcttgt ctggcaaagg catacgtgtt tttttcatta gccgtaaatg cacatactaa
    15481 agaacttaac taaacttcac atcgccgctt catttttcgc ccgtagccca gtacaaagaa
    15541 atagttcacg ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac
    15601 gctggaaatc agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc
    15661 tttgatggtc atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat
    15721 catcagtaag ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg
    15781 caactccacc acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt
    15841 ggtggtaata ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc ccagacgaga
    15901 gtacaaacga tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc
    15961 gtagcagata aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa
    16021 gcgggcgagc aggtcatagc agccgtaaat cagatagctg acgaccacca gcgaccgcac
    16081 taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt
    16141 ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct
    16201 tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc
    16261 agtttctacc cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag
    16321 acgtggcgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa
    16381 cgccagcacg cttttggtca ggttccacct ggtgcgtttg ggcagcatgg tttcatccac
    16441 ctgctgacaa tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt
    16501 acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt
    16561 actggaccct actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg
    16621 gcggcggtac tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc
    16681 gccgactctg acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc
    16741 gcggcgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt
    16801 ggcgataatg acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc
    16861 atcgcggtgt tcttcgttat cgcgccagac cagcaatacc tgcgcgtctc ccggctggcg
    16921 gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag
    16981 gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta
    17041 atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc
    17101 aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt
    17161 ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc
    17221 cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac
    17281 ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac
    17341 gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc
    17401 cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc
    17461 gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt
    17521 cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc
    17581 ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg
    17641 cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc
    17701 cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat
    17761 tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac
    17821 atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc
    17881 ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac
    17941 agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg
    18001 ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc
    18061 agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg
    18121 gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct
    18181 ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt
    18241 ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg
    18301 tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga
    18361 gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga
    18421 ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg
    18481 ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt
    18541 ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca
    18601 cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa
    18661 acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt
    18721 tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac
    18781 cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc
    18841 gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac
    18901 gtcaggtttt gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga
    18961 atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt
    19021 gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag
    19081 aacagcgcca gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca
    19141 atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc
    19201 caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg
    19261 acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc
    19321 accacaaacc atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg
    19381 ttgttaggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc
    19441 aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac
    19501 ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt
    19561 aacgcctttt gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca
    19621 tgggtaaagg cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta
    19681 tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac
    19741 aggatcacct gaataagcac aggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc
    19801 aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta
    19861 atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat
    19921 caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg
    19981 cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg
    20041 catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagga acgcggcgtt
    20101 cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat
    20161 ccccagggta ctgagtaaaa acaggctgga gataaaaaac agaataagca gcgaaccgcg
    20221 atacggcacg ccgagaataa acactgacac cagcatacac agcagcatcg ccagcatccc
    20281 gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga
    20341 gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat
    20401 cgcgccgatg accgtcatga tgatggtcac tgcaccgggg ataatgaagt gctggctaat
    20461 cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc
    20521 gttgtcctcc gctcgctgca tttgccagat ctgccagatc tgccagatcc cttcgacata
    20581 cccctgtaca aagttagcgg tattcggctc actgccgtcg gtgatcacct gaatcggtgc
    20641 ggtggcgttg gcgcgctcca tctgttccgc aaaatccacc ggaataacca ccagaccgcg
    20701 aattttcccc gcctgcattt tggcgatcag ttcctgacgg ttatcgctga tggtggcgtc
    20761 gatgtagggc gaaccggtca tggtgtgggt gaaatccagc gccgcttcgc tacgctgttc
    20821 cagcaagatc ccgacccgca gcttgctgga gtcgaggtta atgccataac caaaaataaa
    20881 cagcagtagc agtgggatca ctaccgcaat cagccagcta ctcggatcgc gaacgatctg
    20941 ccgcgtctct ttgacgcaca gcgcccgcac gcgacgccag gaaaggatcg ggttactcat
    21001 tgctatgctc cttatcccag tcgtggatca actgaataaa ggcttgctcc atggtgggat
    21061 cgggttgctc atcgttagcc gactgtgctt tcaaatcgtc cggcgtaccg ctggcgatta
    21121 atttcccacg gtacaccagg ccgatgcggt cgcaatattc cgcttcatcc ataaagtggg
    21181 tggtgaccat caccgtgacg cctttctcta ccatgctgtt gatatgtagc caaaattcac
    21241 ggcgggtgag gggatcaacg ccggacgtcg gttcgtcgag aaacagaatg tccggttcat
    21301 gcatcagcga acaggccagc gccagccgct gtttaaaacc taatggcagt tcatcggtgg
    21361 cgtgggaggc gatacttttc aggccgaacg cctcgctcat gcgggagatt ttttcgttct
    21421 gcgcccgacc gcgtaagcca tacacaccag agaaaaagcg taaattctgt tcgaccgtca
    21481 ggttaccgta gagcgaaaac ttttgcgcca tatagccgag atgctggcgc gctttaccag
    21541 aactctcttt cagatccatc cccagtacca gcgcctgacc agaggtcggc accagcaaac
    21601 cgcacatcat cttaaaggtg gtcgatttgc ccgcgccgtt tggccccagc aaaccaaaaa
    21661 tctccccgcg tttaacggca aagttgacgt gatcggtggc ggcaaaatca ccaaatttct
    21721 tggtcagttc tttcgcttcg atcaccgtct cgccgggtgt gccttctacc gtatgtaata
    21781 ttgcgcccag cggcgattcc gaggttccgg caccgcccag caaatcaata aacgcatctt
    21841 caaaacgcgg cgtagtttcg ttgatgttga tttccggcat cccgtcggca tggcgaatat
    21901 cgtctggtgt ggtctctttt ttgaggatca gacgtaccga tttcccctga atcatgccgt
    21961 cgctgacctg cggcagtttc aatgcccgtt gcaacagttt gcggttgccc tcgtgtggac
    22021 ttgtcatcag aaagctgcgt ccggccatgg tttgtgtcag ggcttttggt tccccctgat
    22081 acagcaactc gccttcattc atcagtaaca cgtcacggca ctgctcggct tcgtcgagat
    22141 acgaggtact ccagaggatt aacatccctt cgcctgccag ctcatgcacc atctgccaca
    22201 gttcgcgccg tgagataggg tcaacgccga cgccgggttc atcgagcagc aacactttcg
    22261 gttcgcccac cagggtacag gccaggccga gtttttgttt catcccaccg gagagcttgc
    22321 ccgccaggcg tccggtaaac ggcccaagag acgtaaactc cagcaggcga gcaaaagttt
    22381 gcttacgtgc ctcgccggtg acgctgcgca aatccgcgta cagattgagg ttctccatca
    22441 ccgtgagatc ttcatacaga ccaaatttct gcggcatata gccgagcacg gcgtgcagcg
    22501 cgccgtcgtt tttgattgga tcaaagccaa tcaccgtggc attgccgctg tcgggtttca
    22561 gtaatcccgc cagcatccgc attagcgtgg ttttacctgc accatccggc cccaccagcc
    22621 ccgtcacata accggcgtga atggtacaat cgagcggcgc gacggcgggc ttgtccatgc
    22681 ccggaaagcg tttttccagg ccgttcagcg tgataacggc atcattcatg tcctgcctcg
    22741 ttaccgaatt gcaccgtcac cggcattccc tggcgtaacg catcatcggc gtcggtcacc
    22801 acaatacgca ggcgatagac gaggtcggta cgcagatccg gtgtttcgac ggttttcggg
    22861 gtaaattcag cagtcggcga aacgaaacca atctgcccgt gatacggctt gtccgggcga
    22921 ccatcggtat aaagcagcac tttgcgcccc ggctgggcct ggtcaagata ccgttcatca
    22981 acataagcgc gcacccacac cggacgcgtt agtgaaacgg taaacaccgt gccaccttca
    23041 ttgaggaccg tgcctggctc caccgcgcgc gttaacagcg tgccatcaga cggggctatc
    23101 aacgttgagt cctgtaaatt caactccgcc tgcgccagtt gcgcctgcgc ctgttcgagg
    23161 ctggctttcg cctgggcgat gtcctgttca cggttaccgg aacggtactg acgcaattta
    23221 tcctgtgctg atttcagcgt tgcctgcgcc tggtcgcgcg aggagcgggc attttccagg
    23281 tcatttgccg aaatagtgcg gcttttccac agtccttgct ggcggttata gaagttctgc
    23341 gcatagtcat aggcggcttg cgcctgtttc accgctgcgg cggcctgagc gatttcttca
    23401 tcgcgatacc cggcaagcat cagatcatac tgcgcctgtg ccaccgagac gcccgctttc
    23461 gcctgcatca gggcaatctc atacggcttg tgatccagtt cgcccagcac ctggcccgct
    23521 ttgatagcat caccttcgtc caccgccagc gattcaacgc gccccccaac acggaaacta
    23581 agattcaccg tacgaatatc cacgttgcca tacagcgtca ggccgttatc ctggcggctt
    23641 tgataccacc agtagcctcc ggcaaccacg gcggcaagta ccactaccgc cagttcgatc
    23701 acgacaggtt ttttcatcac tacaaactcc tttgcgataa tccttgcaaa atcaggtcga
    23761 tatgacaggt caccgtctgg ttgatcagtt cggtcttttc ttcatcgaac gcggtccagc
    23821 cggtacgtaa cagaattgtt tctttgccaa gacggaacgc cagaatctca ccaatcaacg
    23881 catgggtatg aaggatcatg cgggtgtcat tggcgtcgct gccggtccag gcggcaatca
    23941 ggcgtgtcag gtggctgtgt agcggactaa tcacctgttc gtgcaccagg tggtaggctg
    24001 ccgtgggaga gagctgctca cgggagataa acttgctgag gttgacggtg tcatcctggg
    24061 tgagcagctt aatcatgttc ctgcaggcgc gaaggagcag ttcacggatg gcagcccgat
    24121 caggctgtgg ttgggcgaac aagcgttcgg cttcctcggc atgcggacgg aactgctcgc
    24181 caataaaatc ggcaatccac tgggcgcagg cgaggtacaa atcttctttc gaaccgaagt
    24241 agtaggtgat ggcagcgata ttctgcccgg cttgggcggc tatctcgcgg gtggtggcgt
    24301 tcattccata ttcaccaaac tgcgccagtg cggcagcaat cagctgtttt ttcgcctgtt
    24361 cacccttgat tgtcatggca ggattattca tcgcacagtc cattcttaat caaatgattg
    24421 attaagatta tgactccata tgggagttgt ccagtatggc taagaatttt agctacgcca
    24481 gtcacaggga taatttatgc gctgcgtcac aaaaactgct acactccgct cgctcatgac
    24541 attgtggttt ttgtcatttt ccttttcagt atctccctga aaactacacc ggtaacggtc
    24601 ggggcggttc ggagtagtca tgtctttcga ttatttgggt ttaagccctg atatcctgcg
    24661 cgccgttgcc gagcagggtt accgtgaacc cacccctatt cagcagcagg cgatccctgc
    24721 ggtgctggaa ggccgcgacc tgatggctag cgcccagacc ggcaccggca aaacagcggg
    24781 ctttacgctg ccgctgttgc aacacctgat cactcgccag ccgcacgcca aagggcgtcg
    24841 tccggtacgt gcgctcattc ttaccccgac ccgtgaactg gcggcgcaga ttggcgaaaa
    24901 cgtccgtgat tacagcaaat acctgaacat tcgttcgctg gtggtgtttg gtggtgtgag
    24961 tattaacccg cagatgatga aactgcgtgg cggcgttgat gtgctggtgg caacgccggg
    25021 acgtttgctg gacctggaac atcagaatgc agtgaagctg gatcaggttg aaatcctcgt
    25081 cctcgatgaa gctgaccgca tgctcgacat gggctttatc cacgatatcc gtcgcgtgtt
    25141 aacaaaacta cctgcgaagc gccagaacct gttattctcc gcgaccttct ctgacgatat
    25201 taaagccctg gcggaaaaac tgttgcacaa cccgctggaa atcgaagtgg cacgccgcaa
    25261 taccgcgtct gatcaggtga cccagcacgt tcactttgtc gataagaaac gcaaacgcga
    25321 attgctgtcg cacatgattg ggaaagggaa ctggcagcag gtgctggtgt ttacccgtac
    25381 caaacacggc gctaaccatc tggctgaaca gctcaataaa gatggcatcc gtagtgcggc
    25441 gatccacggc aataaatcgc aaggtgcgcg tactcgtgcg ctggctgatt ttaaatcggg
    25501 cgatattcgt gtactggtgg caactgacat cgctgcgcgc ggcctggata ttgaagagct
    25561 gccgcacgtc gtcaactatg aactgccaaa cgtaccggaa gattatgtcc atcgtatcgg
    25621 gcgtaccggt cgtgcggctg ctaccggtga agcgttgtcg ctggtgtgtg ttgatgaaca
    25681 caaactgctg cgtgatatcg aaaaactgct gaaaaaagag atcccgcgca ttgcgattcc
    25741 gggctatgag ccggacccgt caatcaaagc cgaaccgatc cagaacggtc gccagcaacg
    25801 tggcggcggc ggtcgtgggc aaggtggtgg tcgcggtcaa cagcaaccac gccgtgggga
    25861 aggtggcgca aaatctgcaa gcgcgaaacc tgcagaaaaa ccgtctcgcc gcctcggcga
    25921 tgccaaaccg gcaggcgaac aacaacgtcg ccgccgtccg cgtaaacctg ccgctgcgca
    25981 gtaatctttt atgccgggct atgcccggca tcaggctgat gaacaaacgc aaaactgcct
    26041 gatgcgctac gcttatcagg cctacgtgaa ctctgcaata tattgaattt gcatgctttt
    26101 gtaggccgga taaggcgttt acgccgcatc cggcatttca caacaagcac ttgtcagcaa
    26161 tttgagaaca cgggaaaaaa tttatctgtt ttaccgccca taagccaccg agacgttact
    26221 tctctatagc caattgttct cgcaactcca ttaaaaggta gcccagtcga ttcttgccct
    26281 taccattacc accgtctccc cagtaagcat cgttttccgt atgctcaacc agttttgcgg
    26341 gcgcggttgc cagcaagagc acacgcagtt ctgcatgctg ttcgaattta gcacgaagtg
    26401 ctttacgcat cacctgttct ttaaccgact cccagttttt acgaagtggc ttggaacgat
    26461 tacgccccat gcgagcggcg accatggggg aagaaacccg acggatctct tcgcggtatt
    26521 tttcatcaag gaatttttgt gcctgaaaat agtgttcgga ggtgggccag gttttcccgt
    26581 caaccttgat gggccaggcg gcaaagttag aaaaatcacc gtagtcatcg ctggtgctgt
    26641 agaaatttat gatggtgtct tgcatgacgt gttggatcct ttgtgctcga acgggcatta
    26701 aaccgcatta tgttggtggt tattgcgagc cgctttccag aaacagaaaa accattaccc
    26761 ctgaaaaccg aaaaatgcca caatattggc tgtttataca gtatttcagg ttttctcatg
    26821 gcattaaccg ccgcgcttaa agcgcaaatt gccgcctggt ataaggcgct tcaggaacag
    26881 atccccgact ttattccccg tgtgccgcag cggcagatga ttgcggacgt cgccaaaacg
    26941 ctggccggag aagaagggcg gcatctggcg attgaagccc ccaccggcgt tgggaaaacg
    27001 ctctcctatt tgatccccgg catcgccatt gcccgcgaag agcaaaaaac gctggtggtg
    27061 agtaccgcca acgtggcgtt gcaggatcag atttacagca aagatttacc gctgctgaaa
    27121 aagatcattc ccgatcttaa attcactgcc gctttcgggc gtgggcgcta cgtttgtccg
    27181 cgtaatctga cggcgctcgc cagcactgaa cccacgcaac aggatctgct ggcgtttctt
    27241 gacgacgaac tgacggcaaa taatcaggaa gagcaaaaac gttgtgcgaa gctgaagggc
    27301 gatctcgaca cttatagatg ggatggtctg cgcgatcata cggatatcgc tatagatgat
    27361 gatctctggc gtcgtttgag taccgacaaa gccagctgcc tcaaccgtaa ctgttactac
    27421 tatcgtgaat gcccgttttt tgttgctcgt cgggagattc aggaagcgga agtggtggtg
    27481 gcaaaccatg cgctggtgat ggcggcgatg gaaagcgaag ccgtactgcc tgacccgaaa
    27541 aatctgctgc tggtgctgga cgaaggtcat cacctgccag atgtggcccg ggatgcgctg
    27601 gagatgagcg ctgaaatcac cgcgccctgg tatcgactac agctggactt gttcacgaaa
    27661 ctggtcgcta cctgcatgga gcagtttcgc ccgaagacca tcccaccgct ggcgatccct
    27721 gaacgtttga atgcgcattg tgaagagttg tatgagctta tcgcctcgtt aaacaacatt
    27781 ctcaatctct acatgcctgc cgggcaggag gcagagcacc gttttgcgat gggcgaactg
    27841 cctgatgaag tgctggagat ctgccagcgg ctggcaaaac tcaccgagat gctgcgtggt
    27901 ctggcggagt tatttcttaa cgatttaagt gagaaaaccg gcagccatga cattgtacgc
    27961 ctgcatcggt tgattttgca gatgaaccgc gcgttgggga tgttcgaggc gcaaagcaaa
    28021 ctctggcggc tggcgtctct ggcgcaatct tccggtgcgc cggtgaccaa atgggcgacg
    28081 cgggaagagc gcgaagggca actgcatctc tggtttcact gcgtggggat acgtgtcagc
    28141 gatcagctgg aaaggctgct gtggcgcagt attccgcaca ttattgtcac ctccgcaacc
    28201 ttgcgttcgc tgaatagttt ttcgcgtttg caggagatga gtggtctgaa agagaaagcg
    28261 ggcgaccgtt ttgtggcgct ggattccccc tttaaccact gcgaacaggg caaaattgtt
    28321 attccccgga tgcgcgttga gccttccatc gacaacgaag agcagcatat tgccgaaatg
    28381 gcggcctttt tccgtaagca ggtggagagc aaaaaacatc tcggtatgtt ggtactgttt
    28441 gccagcggac gggcgatgca gcgctttctc gactatgtga cggatttacg tctgatgttg
    28501 ctggttcagg gcgatcagcc gcgttaccgt ttagttgaac tgcaccgcaa acgcgtcgcc
    28561 aacggtgagc gcagcgtgct ggtgggctta cagtcatttg ccgaagggct tgatttgaaa
    28621 ggtgatctgc tcagccaggt gcatatccac aaaatcgctt ttccgcccat cgacagcccc
    28681 gtggtgatca ccgaagggga atggctgaaa agcctcaacc gctatccgtt tgaggtgcaa
    28741 agcctgccga gcgcctcgtt taacctgatt cagcaggttg ggcgactgat tcgaagccac
    28801 ggttgctggg gcgaagtggt tatctacgac aaacgcttgc tgaccaaaaa ttatggcaag
    28861 cgactactgg atgcattacc ggtatttccg atagagcaac cggaagtccc tgaaggtata
    28921 gttaaaaaga aagaaaaaac gaaatcccca cgccgtcggc ggcgttaatg atgtgagtca
    28981 ggtaaggagt cgtaaatgga ctatcgcaaa atcattaaag agatcgggcg cgggaaaaac
    29041 cacgcgcgcg atttagaccg ggatactgcg cgcggtctgt atgctcatat gctcaatggt
    29101 gaagtccctg accttgagtt gggcggcgta ttgattgcgc tgcgtatcaa aggagaaggg
    29161 gaagcagaga tgctcggctt ttacgaagcc atgcaaaatc acaccatcaa gctgacgccg
    29221 ccagcaggca agccgatgcc gattgtcatc cccagttaca acggcgcgcg taaacaggcc
    29281 aacctgacgc cgttgttggc gattctcctg cacaaactcg gttttcctgt ggtggttcac
    29341 ggggttagcg aagatccaac ccgcgtgctg actgaaacca tttttgaatt gatgggtatt
    29401 acgccaacgt tgcacggcgg gcaggcgcag gcgaagctcg acgaacatca accggtgttt
    29461 atgccagtcg gcgcgttttg cccgccgctg gaaaaacaac tggcgatgcg ctggcggatg
    29521 ggcgtgcgta acagtgcaca taccctggcg aaactggcga cgccatttgc cgaaggtgcg
    29581 gcgctgcgtc tttccagcgt ttcgcatccg gaatatattg gacgcgtcgc gaagttcttt
    29641 agcgatatcg gcgggcgggc gctactaatg catggtactg aaggtgaagt gtatgctaat
    29701 ccgcagcgct gcccgcagat caatctcatt gaccgtgaag ggatgcgggt gctgtatgaa
    29761 aaacaggaca ctgctggtag cgagttactg ccacaagcaa aagatccgga aaccacggcg
    29821 cagtggattg agcgttgcct tgctggcagc gaaccgattc ccgaatcgct gaaaatccag
    29881 atggtttgct gcctggtggc tatgggtgaa gcggcaacta tcagcgacgg cctggcgcgc
    29941 gttaatcagg cattttaatt cttttctcag ccggatgagc catgtcatcc ggcttttacc
    30001 ccaccgcaat atgaaattcc tgcatcttta ttgaccttcc cacgcccggc gtgcagcata
    30061 aaaatacaac aaacacataa cataaacagg agttaaccat ggaaagtggt catcgctttg
    30121 atgctcagac gctgcacagt tttattcagg ctgtatttcg tcagatgggt agcgaggaac
    30181 aagaagcgaa attagttgcc gatcatttaa tcgcggcaaa cctggcaggg catgattcac
    30241 atggtattgg catgatccca agctatgtac gctcctggag tcaggggcac ctgcaaatta
    30301 accatcatgc caaaaccgtt aaagaggcgg gggcggcggt cacgctcgat ggcgatcgcg
    30361 catttggtca ggtcgcggca catgaagcga tggcgctggg gattgagaaa gcgcatcagc
    30421 acggtattgc cgccgtggcg ctacataact cgcatcatat cggccgtatc ggttactggg
    30481 cggagcagtg tgcagcggcg gggtttgtct ctatccactt tgttagcgtg gtcggtattc
    30541 caatggtcgc gccgttccac ggtcgcgaca gccgctttgg caccaatccg ttctgtgtgg
    30601 ttttccctcg taaagataat ttcccgctgt tgcttgatta cgccaccagc gccattgcat
    30661 ttggcaaaac ccgcgtcgcc tggcataaag gcgtccccgt gccgccaggt tgcctgattg
    30721 acgttaacgg cgtgccgacg accaatccgg cggtaatgca ggagtcgccg ttgggttcgc
    30781 tgttgacctt tgccgaacat aaaggctacg cccttgcagc gatgtgtgaa attcttggcg
    30841 gggcgctttc cggcggtaaa acgacgcatc aggaaacgtt acaaaccagt cccgatgcca
    30901 ttcttaactg catgaccact atcatcatca acccggaact cttcggcgcg ccggattgta
    30961 acgcgcagac cgaagccttt gccgagtggg tgaaagcctc gccacatgat gatgataagc
    31021 cgattttgct accgggcgag tgggaagtga acacgcgtcg cgaacggcag aagcagggga
    31081 ttccactgga tgcgggaagc tggcaggcca tttgtgatgc agcgcggcag attggtatgc
    31141 cggaagagac gttgcaggct ttctgtcagc agttagccag ctaaaaaaaa gcccgtccag
    31201 tggcggacgg gcaaacaagg gtaacatagg atcaatgagg gttagagcat atgcgtctgt
    31261 cggcaaacag acagggaaat acttgtgctg gacgtagcgt aaacgcctga tccgtccaac
    31321 ggttcggatt ttgtaggcct gataagacgc ggtagcgtcg catcaggcat ctgttgccgg
    31381 atgcggcgta aacgccttat ccgtcctacg aattacttat aaattaccgc agtaccgctt
    31441 aatttattgt tgttggtggc ggaagtgatg ctgtatccac tggcaccggc tgccgcggct
    31501 ttctcagcca gtttcgcttc cagggcatcg agggtggatg cgccatcggc agaaaccacg
    31561 ccgattttat tcatgttctg tgcctgggat gccgttatcg gttccgccgc gaacacgcca
    31621 aatgacagag ttgaaagagc catagcagca acaacagtat tgatagtttt cataattaat
    31681 ctctcgcagg tgatttttgt ataagtgacg ttgtttcgtc gatgtgatga gtatcacgtt
    31741 tttttacgag agataaaatc gaagagaatt gaccgccttg ttcaaataaa ttgattgata
    31801 tctaacatac tgataaacca tttatttgtt gtacttatga acttgtttta taccgcgtgg
    31861 cagtcacagc agcgttaaag gcacggaatg ccacgcaaaa taaaaattat actcgctatg
    31921 catctcgcca gttgtagtta acgtaactgg ctgtctttcg aacctctgcg attatatcct
    31981 gtataagctg gtttttgtaa atctttctcc tgctgacaat gaatacataa gcgcacgcca
    32041 ggaatggctt cccgacgggc ctgcgggatg ggggcaccgc actcttcaca ttcatccagg
    32101 ctttcgccgc gcggaatttc accccgagcg cgggcaatcg cattttcaat tgtactgttg
    32161 atctgttcgt tgacggcgtc atcgttagcc caaccggatg ccatatcgac ctccccatat
    32221 caatacttgt acagttaagt gtagctaatc cagggacgaa ctcgggcagt tcaagcatca
    32281 gatctccgac cattcccgca gcagattatg ataaagatta agcagcgaca ggatctcttc
    32341 actttcgccg tagcggcttt tcagcgactg aatattgttg tccagttcaa acagcatggc
    32401 gcgcttttta tcatcgcgga tcatcgactg gatccacata aatgatgcca ctcgtacgcc
    32461 gcgggttacg ggtggcacgc aatgctggct gctggaggga tacaacacga gatcgcctgc
    32521 cgggagtttt acccgatgtt gtccgaaggt gtcattaacg accagttcgc cgccgtcgta
    32581 gctttgtgga tcgcttaaaa acagcgtggc agaaaggtca gtacgcatcc agccgttttg
    32641 tggatggctg cgtactgcgc catccacatg gaaaccatag gtttcattgt tctgatagcg
    32701 attaaacagc ggcgtggaaa gggtacgcgg caaggccgcg gcaaagaaca aagcatgttg
    32761 gttaaccgcg ttcagcacct cattttgcag ggcggcgtat aacgtgctgc gggtgtcgac
    32821 ctgttgattg ttcttaactt gcgcaccttg tgcgccggtg gtgacgcgtc catccaccca
    32881 ttcggcttgt tccagttgtt cgcgaaaccg ggcgacgtcc tgtggcgata acacgccggg
    32941 aatgtggtac atcatacaag ttctccaaaa agtggggcct gcgccccaca tctgaatcag
    33001 aaatgcatat tggctgtgag caagaaggtt cttggctcgc ccgggtgata acggtagccg
    33061 ctcttgttga ttgaggcgac gtaatcagta tcaaacaggt tgtagacgtt tagctggaag
    33121 tcgagattgc gattaacccg ataccccagt ttggcatcgg cgacccagta accttcggta
    33181 aatgctggcg ttcccaccgc gccgtctgaa cctttatgca tactgccgat atagcgtgcg
    33241 cccgcgccaa cagagatatc gtcggttgcc tgatattggc tccataaggt gaaggcgtgc
    33301 tccggggtat acggcagcga tgaggaacca tcctgggcaa catctttgcc gtttttgatg
    33361 gttgcttttt gctgggtata gccgccaatc acctgccacg cgggagtgat attcccggcc
    33421 acggatatct catagccttc gacgcgtttc ttaccgtatt gcgagtaagt tccgtcatca
    33481 ttttgctcaa cttcattttc gatatcagta cggaacagcg cggcggtgag caacagacgt
    33541 ttatccagaa cctgccattt ggtgccaatc tcgctggtgt tggctttttg cggtttaaaa
    33601 tcggtgcggt tggcactgtt accgctgcca gactgcgcaa gggcgaagtt gttgccgccc
    33661 ggaggctgct gggaaacggc atagttaata tagacattgc cgttttccgt caggtgatac
    33721 agcgccccgg ctttccagtt catcagattg cccgacttgg cggtgtcgac ggtggtgacc
    33781 ggagaacctt ttgccacacc agttgggcag gtgatggcac cgcgtccgct gccgccgcag
    33841 gcggtggcac tgtcatattc agtatgataa ttatccagac ggatcccgcc gttcagctca
    33901 aaatcacggg tgatttgcag cgtatcaaag gcgtaaattg cgaaggtatc cgtctgacca
    33961 ttggcgtttg cgccgttgcg cgtcaggccg ccgggatgaa tgctgctgtc aggatgataa
    34021 atatttaccg cgggtaacgt caccggatta acgccgtagt tagtctgcgt ttcacgggta
    34081 aattccacgc cggtactgac atcatgaccg atagaaccgg tatagaacgt cgaggtcagg
    34141 ttggtctggt tggtgagaat tttattactc acatctttgg tattcgccgt gcgtgaccag
    34201 gttcagctat tcacatcgct ggtgggctga gtaatattcg acgccccgcc cataatcgcc
    34261 gtcatcaggt aatcctgctt tacgcgcgac caacgggtag tattgcgaat ggtggtgtta
    34321 tcgttgatgt cgtgctcaaa acgcatggtg gcggtgtcgg tggtcgaatc gtcgtaatcg
    34381 gaatccgtgc cgtaaaagtt atgagtatca acttttccgg aatgattcag ggccgccgtt
    34441 cccgcagatg gggcagaata gcccggcaaa ccgatggtcg gaatgccgcc gtctggcgtg
    34501 ttgtgctggg tgacatgcag ataattaaga tacaaacgat tcgctgtacc aaggccaaaa
    34561 gcgacagaag gggcgacgcc gtaacgctca tttttgactt tgtcgcgtcc ggcatcgtgc
    34621 gttttttcgc ccattacatt caggcgcacc gcggtggtat caccaatgac ctgattgacg
    34681 tccagcgtgc cgcggcggaa ccaggcgctg ccaatactgg cggaggcgtc aatgccggaa
    34741 tcattgcgcg gctgtttgct gatcatattg atcgagcccg tcggtgcgct gcgcccgtag
    34801 tcggtgccgg acggcccttt aatcacttcg acctgctcgg tattgaaggt gtcgcgcgag
    34861 acgctgccga tatcgcgaat gccatcaata taaatactgt tagaggtatc ggcaccacgc
    34921 atataaatgg cgtcgccagt ggtggagtta ccgttctcac ccgcaaaaaa cgcacccacg
    34981 c
//
LOCUS       DAZOZY010000022        34132 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZOZY010000022 DAZOZY010000000
VERSION     DAZOZY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34132
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.022"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHURQ_000946"
     CDS             38..580
                     /locus_tag="ACHURQ_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822791.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="ACHURQ_000947"
     CDS             663..1364
                     /locus_tag="ACHURQ_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0822792.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="ACHURQ_000948"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="ACHURQ_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="ACHURQ_000949"
     CDS             3979..5049
                     /locus_tag="ACHURQ_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822793.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="ACHURQ_000950"
     CDS             5061..5603
                     /locus_tag="ACHURQ_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822794.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="ACHURQ_000951"
     CDS             5611..6126
                     /locus_tag="ACHURQ_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822795.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="ACHURQ_000952"
     CDS             6092..6829
                     /locus_tag="ACHURQ_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0822796.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHURQ_000953"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHURQ_000953"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR0822797.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHURQ_000954"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHURQ_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR0822798.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHURQ_000955"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHURQ_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR0822799.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHURQ_000956"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHURQ_000956"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR0822800.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHURQ_000957"
     CDS             12136..14043
                     /locus_tag="ACHURQ_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0822801.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHURQ_000958"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHURQ_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR0822802.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHURQ_000959"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHURQ_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR0822803.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHURQ_000960"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHURQ_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR0822804.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHURQ_000961"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHURQ_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR0822805.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHURQ_000962"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHURQ_000962"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR0822806.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHURQ_000963"
     CDS             complement(18711..20471)
                     /locus_tag="ACHURQ_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0822807.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHURQ_000964"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHURQ_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR0822808.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHURQ_000965"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHURQ_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR0822809.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHURQ_000966"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHURQ_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR0822810.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="ACHURQ_000967"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="ACHURQ_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR0822811.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHURQ_000968"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHURQ_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR0822812.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="ACHURQ_000969"
     CDS             complement(26072..26518)
                     /locus_tag="ACHURQ_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR0822813.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="ACHURQ_000970"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="ACHURQ_000970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR0822814.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHURQ_000971"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHURQ_000971"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR0822815.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="ACHURQ_000972"
     CDS             complement(29281..29943)
                     /locus_tag="ACHURQ_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR0822816.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30116..30529
                     /locus_tag="ACHURQ_000973"
     CDS             30116..30529
                     /locus_tag="ACHURQ_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR0822817.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHURQ_000974"
     CDS             complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHURQ_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR0822818.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHURQ_000975"
                     /pseudo
     CDS             complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHURQ_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32234..32512
                     /gene="yccX"
                     /locus_tag="ACHURQ_000976"
     CDS             32234..32512
                     /gene="yccX"
                     /locus_tag="ACHURQ_000976"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR0822819.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHURQ_000977"
     CDS             complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHURQ_000977"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR0822820.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHURQ_000978"
     CDS             complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHURQ_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR0822821.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33795..33882)
                     /locus_tag="ACHURQ_000979"
     tRNA            complement(33795..33882)
                     /locus_tag="ACHURQ_000979"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33846..33848),aa:Ser,seq:tga)
BASE COUNT         8829 a         8131 c         8898 g         8274 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcttggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
    30061 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
    30121 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
    30181 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
    30241 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
    30301 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
    30361 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
    30421 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
    30481 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
    30541 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
    30601 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
    30661 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
    30721 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
    30781 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
    30841 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
    30901 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
    30961 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
    31021 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
    31081 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
    31141 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
    31201 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
    31261 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
    31321 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
    31381 ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
    31441 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
    31501 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
    31561 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
    31621 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
    31681 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
    31741 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
    31801 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
    31861 cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
    31921 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
    31981 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
    32041 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
    32101 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
    32161 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
    32221 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
    32281 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
    32341 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
    32401 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
    32461 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
    32521 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
    32581 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    32641 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    32701 cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca ccactgccag
    32761 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    32821 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    32881 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    32941 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    33001 aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg tttcaaacaa
    33061 aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    33121 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    33181 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    33241 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    33301 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    33361 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    33421 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    33481 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    33541 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    33601 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    33661 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    33721 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    33781 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    33841 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    33901 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    33961 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    34021 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
    34081 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aa
//
LOCUS       DAZOZY010000023        33379 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZOZY010000023 DAZOZY010000000
VERSION     DAZOZY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33379
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.023"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..275)
                     /gene="ybgE"
                     /locus_tag="ACHURQ_000980"
     CDS             complement(<1..275)
                     /gene="ybgE"
                     /locus_tag="ACHURQ_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR0822822.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGL"
     gene            complement(275..388)
                     /gene="cydX"
                     /locus_tag="ACHURQ_000981"
     CDS             complement(275..388)
                     /gene="cydX"
                     /locus_tag="ACHURQ_000981"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR0822823.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(403..1542)
                     /gene="cydB"
                     /locus_tag="ACHURQ_000982"
     CDS             complement(403..1542)
                     /gene="cydB"
                     /locus_tag="ACHURQ_000982"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0822824.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1558..3126)
                     /gene="cydA"
                     /locus_tag="ACHURQ_000983"
     CDS             complement(1558..3126)
                     /gene="cydA"
                     /locus_tag="ACHURQ_000983"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR0822825.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(3974..6607)
                     /gene="mngB"
                     /locus_tag="ACHURQ_000984"
     CDS             complement(3974..6607)
                     /gene="mngB"
                     /locus_tag="ACHURQ_000984"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR0822826.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(6625..8541)
                     /gene="mngA"
                     /locus_tag="ACHURQ_000985"
     CDS             complement(6625..8541)
                     /gene="mngA"
                     /locus_tag="ACHURQ_000985"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR0822827.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            8710..9432
                     /gene="mngR"
                     /locus_tag="ACHURQ_000986"
     CDS             8710..9432
                     /gene="mngR"
                     /locus_tag="ACHURQ_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR0822828.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(9536..10405)
                     /gene="sucD"
                     /locus_tag="ACHURQ_000987"
     CDS             complement(9536..10405)
                     /gene="sucD"
                     /locus_tag="ACHURQ_000987"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR0822829.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10405..11571)
                     /gene="sucC"
                     /locus_tag="ACHURQ_000988"
     CDS             complement(10405..11571)
                     /gene="sucC"
                     /locus_tag="ACHURQ_000988"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR0822830.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     CELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(11665..12882)
                     /gene="odhB"
                     /locus_tag="ACHURQ_000989"
     CDS             complement(11665..12882)
                     /gene="odhB"
                     /locus_tag="ACHURQ_000989"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR0822831.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(12897..15698)
                     /gene="sucA"
                     /locus_tag="ACHURQ_000990"
     CDS             complement(12897..15698)
                     /gene="sucA"
                     /locus_tag="ACHURQ_000990"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR0822832.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(15999..16715)
                     /gene="sdhB"
                     /locus_tag="ACHURQ_000991"
     CDS             complement(15999..16715)
                     /gene="sdhB"
                     /locus_tag="ACHURQ_000991"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR0822833.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(16731..18497)
                     /gene="sdhA"
                     /locus_tag="ACHURQ_000992"
     CDS             complement(16731..18497)
                     /gene="sdhA"
                     /locus_tag="ACHURQ_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR0822834.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(18497..18844)
                     /gene="sdhD"
                     /locus_tag="ACHURQ_000993"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(18497..18844)
                     /gene="sdhD"
                     /locus_tag="ACHURQ_000993"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR0822835.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(18838..19242)
                     /gene="sdhC"
                     /locus_tag="ACHURQ_000994"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(18838..19242)
                     /gene="sdhC"
                     /locus_tag="ACHURQ_000994"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0822836.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            19623..19730
                     /locus_tag="ACHURQ_000995"
     CDS             19623..19730
                     /locus_tag="ACHURQ_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822837.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            19936..21219
                     /gene="gltA"
                     /locus_tag="ACHURQ_000996"
     CDS             19936..21219
                     /gene="gltA"
                     /locus_tag="ACHURQ_000996"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR0822838.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            21609..22175
                     /locus_tag="ACHURQ_000997"
     CDS             21609..22175
                     /locus_tag="ACHURQ_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822839.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            22331..24363
                     /locus_tag="ACHURQ_000998"
                     /pseudo
     CDS             22331..24363
                     /locus_tag="ACHURQ_000998"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            24340..24684
                     /locus_tag="ACHURQ_000999"
     CDS             24340..24684
                     /locus_tag="ACHURQ_000999"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR0822840.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            24699..25430
                     /locus_tag="ACHURQ_001000"
     CDS             24699..25430
                     /locus_tag="ACHURQ_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0822841.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            25427..26488
                     /locus_tag="ACHURQ_001001"
     CDS             25427..26488
                     /locus_tag="ACHURQ_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0822842.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            26640..27686
                     /locus_tag="ACHURQ_001002"
     CDS             26640..27686
                     /locus_tag="ACHURQ_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR0822843.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(27683..28474)
                     /gene="nei"
                     /locus_tag="ACHURQ_001003"
     CDS             complement(27683..28474)
                     /gene="nei"
                     /locus_tag="ACHURQ_001003"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR0822844.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(28510..29244)
                     /gene="pxpA"
                     /locus_tag="ACHURQ_001004"
     CDS             complement(28510..29244)
                     /gene="pxpA"
                     /locus_tag="ACHURQ_001004"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR0822845.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(29234..30166)
                     /gene="pxpC"
                     /locus_tag="ACHURQ_001005"
     CDS             complement(29234..30166)
                     /gene="pxpC"
                     /locus_tag="ACHURQ_001005"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR0822846.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(30160..30816)
                     /gene="pxpB"
                     /locus_tag="ACHURQ_001006"
     CDS             complement(30160..30816)
                     /gene="pxpB"
                     /locus_tag="ACHURQ_001006"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR0822847.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(30839..31582)
                     /gene="ybgI"
                     /locus_tag="ACHURQ_001007"
     CDS             complement(30839..31582)
                     /gene="ybgI"
                     /locus_tag="ACHURQ_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR0822848.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            31853..33334
                     /gene="dtpD"
                     /locus_tag="ACHURQ_001008"
     CDS             31853..33334
                     /gene="dtpD"
                     /locus_tag="ACHURQ_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR0822849.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         8180 a         8969 c         8164 g         8066 t
ORIGIN      
        1 agcccgacaa tgagaacaat atcggcaagc aatgggcaaa aaatcccttg ccaaagaacc
       61 ttctgcggac gaaagcccac gccgtgaatc acaccagcac agacggccca catcagcaat
      121 aaaccatgcc agatttccag ttcactggtc ttcgcggcaa aacgagatgg gtcccaaaac
      181 atacatcctg ctaacagaag cgccatcacg aaggaaagcg cccgcagggg gcgcttgtcc
      241 attaccgcat acaaggtcgc gataatctta ctcatcagat gtcttcttga ccggctttgc
      301 ctgattcgac gtgttcaagc gccagcgcgg tgattacccc aaacgaacag gcaagaagcg
      361 ttcccagaat ccatgcgaaa taccacattt ttagctcctt acttagtaca gagagtgggt
      421 gttacgttca atatcttctt tggtgatacg accgaacatt ttccagtaac accaagcggt
      481 gtagagcaga atgatcggta ccagaaccac cgcaacccag gtcatgacgt taagcgtcag
      541 ctggctggaa gttgcatccc acattgtcag acttgcgttc atcatggtgc tggacggcat
      601 cacaaacggg aacattgcga taccggctgt caggatgatg caggccagcg tcagggagga
      661 gaacacaaac gcccacgcgg ctttatccat acgtgcagtc aggatggtca gcagcggaag
      721 aaccacaccc agtgccggaa tagcccacag aattggcgtg ttgttgaagt taaccagcca
      781 tgcgccagct tcacgaacca cttctttatt cagtgggtta gaggctgcgt aatggtccat
      841 tgtcgatttc acgacataac catcgatacc gtacatcacc catacgccag ccagtgcgaa
      901 acagaccagt gtcaccagcg cagccacctg agccgttgca cgggtacgca ggtgcagttc
      961 gcccacggta cgcatttgca gataggttgc gccctgagta atgatcatcc ctacgctcac
     1021 cacgcctgcc agcaggccga acgggttaag caactggaag aagttaccgg tgtagtacag
     1081 acgcagatat tcatcaacgt tgaacggtac gccctgcaac aggttaccga acgctacacc
     1141 aattaccagc ggcggaacga aactaccaat gaagatgccc cagtcccaca tgttacgcca
     1201 gcgggtttct tcaatcttgg agcggtagtc aaaaccgacc ggacggaaga acaaagacgc
     1261 cagcacgagg atcatcgcca catagaagcc ggagaacgca gcggcataga ccatcggcca
     1321 ggcagcaaag agtgcgccgc ccgcggtgat cagccaaacc tggttaccgt cccagtgtgg
     1381 tgcaatggag ttaatcataa ttcgacgctc ggtgtcgtta cgaccgagga aacgggtgag
     1441 catgcccacc cccatgtcga aaccgtcagt gactgcaaaa ccaatcagca gaacgccaac
     1501 cagcagccac cagataaaac gcaatacttc ataatcgatc atttgacgac tcctgtctta
     1561 gcgtgccggc tgagtagtcg tggaagactg ctcaaagtga tagcgaccgg ttttcaggct
     1621 gcttgggccg aggcgtgcaa acttgaacat taagaacaat tctgccacca ggaacagggt
     1681 atacaggccg caaatcagca ccattgagaa gatgagatcg cctgcggtca gtgacgagtt
     1741 cgccacagct gtcggcagca cttcaccgat agcccacggt tggcggccat attcagccac
     1801 gaaccagccc gcttctacag caatccacgg cagcggaata ccgtacagcg cggcgcgcag
     1861 aagccatttt ttctcgccaa tgcggttgcg gatgacactc cagaaagaga gcgcgatgat
     1921 tgccagaagc aggaagccac acgccaccat gatacggaac gcaaagtaca gcggcgctac
     1981 acgcgggatg gagtctttgg ttgcctgttg aatctgcgct tcagtcgcat cagccacgtt
     2041 tggcgtatag cgtttcagca gcagaccgta accgaggtct ttcttcatgc tattgaactg
     2101 gtcacgaacc gcctggtcgg tagaaccaga acgcagttgt tcgagcagag agtacgcctt
     2161 catcccgtta cgaatgcgtt cttcatgctg caccatcagc tctttcaggc cgataaccgg
     2221 ggtatccacg gaacgcgttg caatgatgcc cagtgcgtaa gggatctgaa tcgcaaattt
     2281 gttcgtctcc tcttcctgat caggaatgcc gaacagagta aaggcagcag gcgcaggttg
     2341 cgtttcccac tcggcttcaa tagcagccag tttggttttc tgcacgtcgc ccatttcgta
     2401 gccggattca tcacccagaa caataacaga cagaacagca gccataccga agctggcagc
     2461 gatagcaaag gagcgtttag cgaaggcgaa gtcacgacct ttcagcatat accatgcgct
     2521 gataccgagg atgaacatcg cgccagtcac ataaccagac gctacagtgt gaacgaattt
     2581 cacctgagca accgggttaa gcaccagctc ggagaagctc accatctcca tacgcatagt
     2641 ttcaaagttg aaatcggacg cgattgggtt ttgcatccag ccgttcgcaa ccagaatcca
     2701 cagtgcggac aggtttgaac cgagcgccac cagccaggtg acacacatat gctgaacttt
     2761 acccagacga tcccaaccga agaagaacag acctacaaag gtggattcga ggaagaaggc
     2821 catcagacct tcgattgcca gcggcgcacc gaagatatcc cctacatagt gggaatagta
     2881 agaccagtta gtcccgaact ggaactccat ggtcagaccg gtagccacac ccagagcgaa
     2941 gttgatacca aacaacttgc cccagaactt ggtcatatct ttataaatct gtttgccgga
     3001 gaggacgtag accgtttcca taatggccag caggaacgcc ataccgagcg tcagtggcac
     3061 aaaaaggaag tggtacatcg cggtcaaggc aaactgtaag cgcgacagtt cgactatatc
     3121 taacatcatg actccttgct catcgcatga agactccgag agtgaacccc gttagaaagg
     3181 gtcacacgca tgccccaata gaaatttgtt tgaacacttt catcatacca tgtgaatgca
     3241 cacaaaaacg gcgtaaagaa aggttgcaaa catgttaata aaaactcaaa ttgatcccac
     3301 gtatatatta cgccgcaaaa tccttacaat aaacaggttt ttgttgaagc aaatttgcat
     3361 ttttcgacgg tgatcaattt atagctaaat taccgccttt cagccaattt gatcgagaac
     3421 aatttatctc tttttgatgc caatttccaa gacttataca ttgataaata tcaattccta
     3481 caaattatgt taatcatgtt tcaataaggt aactttagtg acatttatgt ttaaaatgtg
     3541 tgagttataa ggctgaaatt agtttcggtt ggcaatttaa agataaaaag agacgaagca
     3601 aaaacagcac ggttgacctt aattcttttt ttggtcactg gctaatacga cgcagagttt
     3661 caggattact aataaaggca taaatccacc ccgccgatat taacatttaa ttaacttgta
     3721 attatattat aattaactca cccagggtta ttattttgtt aaaaataaag ccacatatac
     3781 gtggcttgag agtgaagata aacgcaaaat tacctgatgc gctacgcttg ccagtgtcta
     3841 catcatctct gcaatatatt gaatttgcct gcttttgtag accgaataaa gcgttcatgc
     3901 cgtatccggc atgaacaaag cgccctttgt caacaatctg gccgcgcata tgtggcttcg
     3961 gtttgattgc tattcaggca agccggtaac tgaacgtccg tgactggccg ggtaaaaaag
     4021 gccccgatag atttgaacct tgattttcct cggtggtaat gtgttcatcc atcatcgttt
     4081 ctgagcaaga aatcacctcg cgactgaaag caacagtcgc atcacaggtt gctgattcag
     4141 ccggattaaa cagccgtaaa atcacttctt gtcggtcttc agctttctta agtgcgctta
     4201 tcaggcatcc cactgggggc attttcaaca aactataact ttccggcacg ttgaatccgg
     4261 ctttgttgag cttcatcgca tcccatggga ttttgttgta acactgtact ggagtcagcc
     4321 atgctcgcgc ctgctgagct acaccagcgg cggttggcgt accggtataa ctcaataaac
     4381 ttaggcgaca agaaagcaga ccacgtagtt gtgagtccgg gactggcatt ttaattcccg
     4441 aaggccgccc aggccttaaa agcagatctt ctttgcccag taagcccacg ccacgcagca
     4501 acgtaatggc aaaggttttc ttctcttcac cgatgacttc aaattcacgt aacccttcgc
     4561 taaagacagc catgccgtta cgcccttcct gtaaggcaac atagttgagc atattccata
     4621 ccggaaccgg cgcttctttc cagccttctt gctgccagtt gttcattgca ctgtcgttca
     4681 cggggcgcgt tagcgaacca aactgcgtat ctgccagaac actgtcagtg ttaaaaggtg
     4741 tagggaccag gacacgaagg cgatgatcgt cagcctggtt atcaaggttg atatccacat
     4801 caatacgcct gctgttatga ctaagagtga caaccaacac cccccctact ctgccagtgg
     4861 attgccgggc gctgcgttct gacaaattga gcggcactgc catgtcatag cggataacag
     4921 ccctgctctg ccaggcttca tgaataatat cgcattgcgg tttcgcgttc gctgcggtaa
     4981 ttacccactc ttcttttgcg ggtgaatagt catattcatc accatcatca gagctttctt
     5041 caatttgcaa tacccggtca tagcgcacac cgctgtcttt atctaccagt tgcagagaac
     5101 catcctcatt gagcgcaatc tgccagaaag cattttccag aatcccttca gcgtcacttt
     5161 ttgccgcaat cacgttgcca ggctgattcg cttcaatata aagcgtgcga tagcccatag
     5221 aagggacaat ctggttgatc tgaatatcaa actccataaa gggatcataa ttgccataat
     5281 gaacaatttg ccgatcgatt aggccaggat cgatctcacg ggcatggcga ataaaatacg
     5341 gtacaggctg accgcgatcg tcttgtagat taaactggct ggcgcgcagc cgcacagtgg
     5401 tgttaataac ttcttcccgc ggccagggca tcaggttaaa cagcacgagt ttgtcggcat
     5461 cactctgtgg catgttatcg gcaattttgc gcatgtagaa acgcagcaga ttatccgcca
     5521 tgtcttcagc cagttcgaag cgggcgacaa tttcgcgatg aactttgtca ctgcaacaac
     5581 aaccgatact gtcgtgggca tgatttttta agatctcttt ccacattttc tccagcaagc
     5641 cgtggtggta ttcaaaaccc aacgtccagg ccagcgttgc cagcggttcc agcagattaa
     5701 caatcttatt ttcaatacgt gcgtgggcaa ttttgatatc catgcgcgtg gaaccgatgg
     5761 tgcgatgtac gcgcatatat ttgccatcga taaattcacc tttcagggtg gccagacttt
     5821 cacgctgcgc ttcgatcttc tcaaatacct cttcaaagcg gctcatcaca aacttacgtt
     5881 gagggtagat ctcacgtagc ttatccatca cttcgaagat attttgctgc aatggcatct
     5941 ggtcatgacc attcggcagc aaaatctctt tggttacaga cgctttttcc agcacgtcaa
     6001 aataactgtc gaggcgttta cgtaatccgt tttcgtcggc aggtaagtac ttaccgatgg
     6061 cgtagcccag cggcagcacc tgcgccgtca cttcgctacc gtcactgctt tgccacaaaa
     6121 actcggtttt atcagtaccg tggcgctccg aacatccgcg ccagaacata gtgcgggtaa
     6181 tgccaaatcc attgtaaata tgcggcagtt gcccggacat gccaaaggaa tccggtaaat
     6241 aacctatttt catcggctcg ccaaacgcga ggcagtcacg cattccgtac atcagattac
     6301 ggacgatgga ttccgcagaa acaatcgtgg tatcggtctg ggtataccag gggccgataa
     6361 tcaacttacc ggcttctacc tgtttcttca cgcggtcttt gttttccggt ttcaccgcga
     6421 aataatcttc gaggatcgcc gtttgcccgt cgagtacgta atatttgtat tcgttgtcct
     6481 gttccagtcg gcacaggatc tcttccatat tattgaccag cagaatgcgt gactcttcgg
     6541 tggtgaaata ccactcccga tcccaatgca tatgcggggt gatgtgaacg cgagataccg
     6601 ctttcatcgt cgtttccttt ttgtttatgg cattacgcca tcagtcagat agttgccatg
     6661 cttaaccgcg tgacgccgcc agatcaggag aattgcagtc gagattgcag cccccaccaa
     6721 tgccgcacca aaccagccaa ttgccgccat aacaccgccc gcgccattat catgaagtaa
     6781 aaagagcgag aaaatgccgg caccgggtgt cgaaaggccg atattcatcg caccgacaat
     6841 agcgcccgtt accatagagc ccagcacaaa cgaaccaata acccgcagcg gatcttcaat
     6901 cgccatcggg atcgcccctt cggtaatacc tgccagccct aacagccagg tggatttccc
     6961 ggtctcaatt tcaaactctt taaacaggcg cggtgcgagc atcgtggaag cggttacggt
     7021 aaatgccgaa accattttga cggaggcgaa aatggcatac gggccgtaaa cgccgttcgc
     7081 cattgcgccc aggcagaatg cataagcggc tttgttcact ggccctccaa ggtcaaagga
     7141 acacataaaa ccgagaatgg cacccagcaa cagcgcgtta cttcctgaca gaccgttcag
     7201 ccaggcggta agcgagttat tgatccatgc gacaggttct cccaccacaa acagcatcag
     7261 actgcccgct cccaacgtac cgagcaccgg gtagagataa aaagtcagga atccattgaa
     7321 tttactgcta agacgcaagt gatttttcac ccagcgcatc aagtaaccgg ctatcaatcc
     7381 gccaacgacc gcgccgagaa acccggagcc gatcatgttg gcggcaagtc cagccgcaaa
     7441 gcctggcgct aacgccggtt tatctgccag agaataggcg gtataggccg cgagcaccgg
     7501 taccatcaaa attccgagca gcccgccgcc cagcttgcgg tacatccaca gccaggagtt
     7561 ttcttcatta aacagatctt gtagcccgaa gatttgcgac agtaataccg cgaccgccag
     7621 caccgtgccc cccgcgacaa tcaacggtac ggcaaaagag attccgctca acagtgcctg
     7681 tttcagctcc gttttgacac ttttcaccgg ttgcgtatct tgctgtacgg tacgcgtctc
     7741 atcgctacgc ttgagggtaa gcgcttgttg gatcaacgct tctgcatggc gaatcggctc
     7801 ggcaacaggc actgaaagcg cgggaatgcc attaaaacgc tcactctcct tgatggcgac
     7861 ttcagccgca aaaatacagg cggttgcact attgagttga tccgccgtta aacgcccttc
     7921 aatgccgtta gcgccttgtt tttcaacgta aacatttacg ccgagtttgc gtccggcttt
     7981 ttccagatat tccgcagcca tataggtgtg agcaataccc gccggacagg ccgttacgca
     8041 gacgatagtt ggcgcgttgg aaaaagaggc agaaggttgc gtgcctccct tgtcatccag
     8101 cgccgagagc aactcatcag gcgtcgtcgc cgactgtata cgcgcccgaa tctcatcatc
     8161 cgcaaggcgc gtggtcagcg ctgtcagcag ttgcatatgc gtagtacccg cttcattggg
     8221 gggaatcgcc agcagcacca ctaaatcaac tgcttccggg ccatcaacgc cttcccactg
     8281 aagcggctcg ctgagtgtgg cgaccgcaaa cgccgcttct tttaccgcag cagttttgcc
     8341 atgcggcaca gccaaccctt cacctaaggc ggtcgggcca aggctttcac ggcgatacac
     8401 ttcttccaga aattgctcag tactggaaat tttccccaga gcagcaagac gttgagtcaa
     8461 cgcgtggatg gcctcttcac ggctggtaaa gcgcgcattc agacacaacg catcgcggtg
     8521 ggtcagagtc gtcagattca tcatgatcct cacttgatcg tttttatagt cgcgccagtg
     8581 tgcccgataa aacaatacca tcatgtgatc aatttcactt gattgaaatt atttgtattg
     8641 tatttgtatt atttacagga tgaatttatc gggcataatg gctaaattcg tattaatgag
     8701 atactaaaaa tgggacacaa gcccttatac cggcagattg ccgatcgcat tcgtgaacaa
     8761 attgcgcgtg gcgagcttaa acctggcgat gcgttaccca ctgaatcagc gctacaaaca
     8821 gagtttggcg tcagccgggt tacggtgcgt caggctttgc gccagctagt cgagcagcag
     8881 atcctcgaaa gcattcaggg cagcgggact tacgtcaaag aagagcgggt caattacgat
     8941 atttttcagt taaccagttt tgatgaaaaa ctgtcggacc gtcacgtcga tacacacagt
     9001 gaaatcctga tatttgaagt gattccggct gacgattttc ttcagcaaca gctacaaatc
     9061 accgcgcagg atcgtgtgtg gcatgtgaag cgcgtgcgct atcgcaagca aaagccaatg
     9121 gcgctggaag aaacctggat gccgcttgcg ttgttcccgg atctcacctg gcaggtcatg
     9181 gaaaattcga aatatcactt tattgaagaa gtgaagaaga tggttatcga tcgtagcgaa
     9241 caggaaatca ttcctctgat gccaaccgaa gagatgagcc gtctgcttaa tatcagccag
     9301 acaaagccga ttcttgaaaa agtatcgcgt ggatatttag ttgatggtcg ggtgtttgaa
     9361 tatagccgca acgcttttaa taccgatgac tataaattta ccctgatagc gcagcgaaaa
     9421 tcatcgcgat aagcacaaaa aaggccatca tacgatggcc aaccatgtcg aaaccgacag
     9481 cccttcgcca ttcctgtaga gggaagcggc gagggctatt tcttattaca gatatttatt
     9541 tcagaacagt tttcagtgct tcaccgatat ccgccaggct gcgaacggtt ttcacgcctg
     9601 cggcttccag agcagcgaat ttctcatccg cagtcccttt cccaccagca atgatcgcac
     9661 ccgcgtggcc catacgtttg cctttcggcg cagtcacacc agcgatgtaa cctacaactg
     9721 gcttggtaac gtgctctttg atgtacgctg ccgcttcttc ttcagcgcta ccgccgatct
     9781 caccgatcat cacgatcgct tcggtctgcg gatctttttc gaacatttcg aggatgtcga
     9841 taaagttaga gcctgggatc gggtcaccgc cgataccaac acaggtcgac tgaccgaaac
     9901 cgtaatccgt ggtctgttta accgcttcat aggtcagtgt accggaacgg gaaacgatac
     9961 ccactttacc cggtttgtga atgtgacccg gctggatacc gattttgcat tcacccggag
    10021 tgataacgcc cgggcagttc gggccgatca tacgaacgcc ggcttcgtcc agtttcactt
    10081 tcacagtcag catatccagc gtcgggatgc cttcagtgat ggtgataatc agtttgatgc
    10141 ctgcatcaat agcttccaga atggagtctt tgcagaacgg tgctggtacg tagataacag
    10201 aagcggtagc gccagtagca gcaacagctt cacgcacggt gttgaacacc ggcaggccga
    10261 ggtgggtagt accgccttta cctggggtta cgccgccaac cattttagtg ccgtatgcaa
    10321 tggcctgttc tgagtggaaa gtcccctggc taccagtaaa gccctggcag ataaccttgg
    10381 tgtttttatc aattaaaatg gacattattt cccctccact gcggcaacaa cctgctgagc
    10441 tgcatccgtc agaccttttg ctgcaataat attcaggccg ctgtcagcca gtttcttcgc
    10501 gccgagttcg gcgttgttac cttccagacg taccacgacc ggtacgttaa cacccacttc
    10561 tgctaccgcg ccgatgatac cgtcagcgat caggtcgcaa cgaacgatac cgccgaagat
    10621 gttaaccaga acggctttca ctttgtcgtc agagaggatg attttgaacg cttcggttac
    10681 acgttctttg gttgcgccgc cgccaacgtc aaggaagtta gccggttcgc cgccgtgcag
    10741 tttaacgatg tccatcgtac ccatcgccag acctgcgccg ttaaccatac aaccgatgtt
    10801 accgtccagc gcaacgtagt tcagttccca ctgtgcagcc tgtgcttcac gcggatcttc
    10861 ctgcgactgg tcacgcattt cgcgcagatc aggctggcgg aacagtgcgt tgccgtcagc
    10921 gcccagtttg ccgtcgaggc aaatcagatc gccctgtttg gtgatgacca gcgggttgat
    10981 ttcgatcaac gccaggtcgc gctccaggaa aatggtcgcc aggcccatga agattttggt
    11041 gaactgctga accagtttac cttccagacc cagtttgaac gccagctcgc atccctgata
    11101 cggcatcggg ccagtcagcg gatcaagcgc aactttgtgg atcaggtgcg gagtttcttc
    11161 cgccactttt tcgatttcca cgccgccttc ggtggaggcc ataaagacca cacgacggga
    11221 actacggtct acaacggcac cgagatacag ctctttagcg atatcggtcg ctgcttcaac
    11281 cagaatctgg ttaaccggtt ggccattggc atctgtttga tacgttacca gacgcttgcc
    11341 gagccagttt tctgcaaaag cacggatgtc ttctttgctg tttacaactt tcacaccgcc
    11401 cgctttaccg cggccaccag cgtgaacctg acatttcact acccacgggc cggcaccgat
    11461 ttttgaagcg gcttcttctg cttcgcgcgg agtagtacag gcataaccca ccggtgctgg
    11521 taagccatag cgggcaaaaa gttgttttgc ctgatattca tgtaagttca tgtgttctgt
    11581 ccatccttca gtaatcgtta tcttttaaac cgtagaccga caccctatcc acgtcggtct
    11641 acagtgcagg tgaagcttaa actactacac gtccagcagc agacgcgtcg gatcttccag
    11701 caactctttg atcgttacca ggaagcccac ggattcgcga ccatcgatca gacggtgatc
    11761 gtaggacagc gccaggtaca tcatcggcag gatctcaacc tgaccattca ccgccatcgg
    11821 acgatctttg atagcgtgca tacccagaat tgcgctctgc ggcgggttga tgatcggcgt
    11881 agacatcagg gaaccgaaca caccaccgtt ggtgatggtg aagttaccac cggtcagatc
    11941 ttcaaccgtc agcttgccgt cacggccttt aactgccagc tctttgattt tcttctcgat
    12001 gtctgccatg ccgagggtat cgacatcacg cagtaccggc gtcaccaggc cgcgcggcgt
    12061 ggaaaccgcc atgctgacgt cgaaatagtt gtggtaaacc acgtcatcac cgtcgataga
    12121 agcattcact tccgggtaac gtttcagggc ttcaaccacc gctttcacgt agaaggacat
    12181 aaagcccaga cggatgccgt ggcgtttttc aaacgcttca ccgtactgct tacgcagatc
    12241 cataatcggc ttcatgttga cttcgttgaa cgtggtcagc atggcggtgg agtttttcgc
    12301 ttccagcaga cgctctgcca cacgcttacg caggcgagtc atcgggacac gtttttcact
    12361 acgtgcagcc agagccggtt gcgccgccgg agcagccgct gccggagcag actctttcgc
    12421 cggggctttc gccagatgtt tttccacatc ttcacgagtc agacgaccac ccacaccggt
    12481 gcctttaatg gcgctggcgt cgagattgtg ttcagccagc aggcgacgga tcgccgggct
    12541 taacgcatcg ttgttttgct cttccagaga cgcctgctgg cgttgcgccg gagtggacgc
    12601 tttctcttca gatttggcgc tggtttcttt accggcgctg ttgccttcac gcaggcgacc
    12661 aaggatctga cgagacgtta ccgttgtacc ttcatcttcc agaaccgcat ccagaatgcc
    12721 gtctgctgat gccggtactt ccagtaccac tttgtcagtt tcgatttcta ccagcacttc
    12781 atcacgtacg actgcgtcgc cgggtttttt atgccaggtt gcgacggtgg catcggctac
    12841 ggattcaggc aggtcaggga ccagaatatc tacgctactc attgtgtatc ctttatttat
    12901 tcgacgttca gcgcgtcatt aaccagatct tgttgctgtt tctggtgaac ggacatatac
    12961 cctaccgccg gagaggcgga ggccgtgcgg cctgcataac gcagagaagc cccaaacgga
    13021 atcacttcac ggaaatgatg ctggctgcag taccatgcgc cctggttgag cggctcttcc
    13081 tggcaccaga caaaatcctt gacgtgagca aactgctgca acacttcctg catcgcttta
    13141 tgcgggaacg ggtagagttg ctcgatacgc acaatggcga catcgtgttg attgttctta
    13201 cgacgctgtt ccagcaggtc gtaataaacc ttaccagaac acatcactac gcgcttcacg
    13261 cccttcggat caagctcgtc gatttcaccg atggctggca ggaaggtgcc gttcgccagt
    13321 tcttcgaggc tggaaaccgc cagcggatga cgcagcaggg atttcggcga catcactacc
    13381 agcggacgac gcatcccgcg cagcgcctga cgacgcagca tgtggtaaac ctgtgccggg
    13441 gtagacggaa cgcaaacctg catgttttgc tcagcacaaa gttgcagata acgttccaga
    13501 cgcgcggagg agtgctccgg cccctgccct tcgtaaccgt gcggcagcaa catcaccaga
    13561 ccacacatcc ggccccattt ctgttcgcca gaagagatga actggtcgat aaccacctgc
    13621 gcaccgttgg cgaagtcacc gaactgcgct tcccagatgg tcagagtgcg tggttctgcg
    13681 gtggcataac cgtattcaaa cgccagcact gcttcttcag acagtacgga gtcccagacg
    13741 cggaacgcgc cctgcccgtt atggatgtgt tgcaacggcg tgtaagtgga accgttagac
    13801 tggttgtgga tcaccgcgtg gcggtggaag aaggtaccgc gaccggagtc ttcacccgac
    13861 aggcgaaccg gaatgccttc gtcaaccagc gtggcgtaag cgaggttttc cgcaccgccc
    13921 cagtcgaaca gtttctcacc ggcagccatc gcctggcgat cgccataaat cttggcaacg
    13981 cgagactgca tttcaactgc ttccggcacc gtgctgatgc gtttcgccag ctcctgcagg
    14041 cgcttcatct caactttgtt cgggtactct tcgtcccatt cgtggttgag gtacggcgac
    14101 caggtgaaag agtgcatgtt catcggacgc cactctgcca ctacgcaatc gccagcatcc
    14161 agcgcatcgc ggtacaggtt aaccatctcg gtggcatctt ccagcgtcgc cactttttcc
    14221 tgctccagct tgtcagcgta gattttgcgc ggtgtcggat gttttttgat tttctgatac
    14281 atcagcggct gggttgcgct cggctcgtcg gcttcgttgt ggccgtgacg gcggtagcac
    14341 accaggtcga tgaagacatc acgtttaaag gtgttacgga aatcgagcgc cagacgggtc
    14401 acaaaggcaa cggcttccgg atcatccgcg ttaacgtgga aaatcggtgc ctgaaccatc
    14461 ttaccgatat cagtacagta cggtgtagaa cgagcatcca gtggattaga ggtggtgaaa
    14521 ccaacctggt tgttgataac gatacgtacc gtaccgccaa cttcataacc acgcgctttc
    14581 gacatgttca gagtttcctg aaccacgccc tgcccggtca ctgcggcgtc accgtggatg
    14641 gtgattggca gcactttgtt gctgctcggc tcatcaagtc tgtccagacg ggcacgaaca
    14701 gaaccgataa ctaccgggct tacaatctca aggtgagacg ggttaaacgc cagcgccagg
    14761 tgcaccaggc cgccatcggt ctggaagtca gacgagaagc ccatgtggta tttcacgtca
    14821 cccgtgccga ggtgttcttt atgtttaccg gcaaactcgt cgaacaagtc ttgcggtttt
    14881 ttacccagca cgttcaccag cacgttcaga cgaccacggt gcgccatccc aagaaccact
    14941 tcgcgggtgc cgctgttgcc agcgtggcgg atcatctctt taagcatcgg gattaacgcg
    15001 tcaccgcctt ccagcgagaa gcgttttgcg ccagggaatt ttgcgccgag gtaacgttca
    15061 aggccttcag cggcggtcag ttcgcttaag aagcgttttt tctcttcgct attgaaagtc
    15121 gcgcgaccag actcgatacg ctgttggatc cagcgttttt cttcggtgct ggtaatgtgc
    15181 atatactcgg caccaatcgg gccacagtag gtttgcttga gggcttccag cagctcgccg
    15241 agtttcatgt tttctttgcc gctggcaaat gaaccgacgt tgaaggtctc ctggaagtct
    15301 gcttcggtca gatcgtggaa agacggatcc agatcggcca ctttatcttg ctgccacagt
    15361 cccagcggat cgagattcgc atgctggtga ccacggaagc ggtatgcgtt aatgagctgc
    15421 aggactttaa cctgcttcac attggtgtca gggtcggaga tcgttgaaga gtaacgtgaa
    15481 gcgtctttcg ccaggcggcg gaaatattca cgcgtttgag agtggaattg atccggtttg
    15541 actcccgtac caggtaactg ctggaacgtc gaacgccagt tagcgtcaac cgagtcagga
    15601 tcggttaaga agtcttcata gagctgttct atccagctct ggtttgcgcc agagaggtaa
    15661 gaagagtcca accaggcttt caaagcgctg ttctgcatcg tgatccctta agcatctttt
    15721 ttatgcttac ttcgccgtgg atactaccac gcacagtgat gtgcgtgccg gggttcactt
    15781 gcgagctctt gtctatgtag tggctcgcga aggaaccttt aaaaactgtc ttagtgtcag
    15841 gggcagtttt taaagatttc ctgcatttct ttgttacgcc tgatgcgctg cgcttatcag
    15901 gcctacgggt aatgacttgt aggccggata aggcgttcac gccgcatccg gcactggttg
    15961 cctgatgcga cgcttgcgcg tcttatcagg cctacggttt acgcattacg ttgcaacaac
    16021 atcgacttaa tatggccgat ggcgcgcgtc gggttcagcc ccttcggaca tacactgacg
    16081 cagttcatga tgctgtgaca gcggaatacg ctgaatgcat cgctcaaacc gtcgaggcgg
    16141 ctgtcagtct cggtatcacg gctatcgatc aggaaacgat atgccgctaa caagcctgcc
    16201 gggccgataa acttatcggg attccaccag aaagacggac aagaggttga acaacatgcg
    16261 cagagaatac attcatacag cccgtcgagt ttttcgcgct gctctggcat ctgtaaatgc
    16321 tcgcgagctg gcggattttg tccattattc aacaggtaag gcttaatttt ctcatattgc
    16381 gcatagaatt gtcccatgtc taccaccaaa tcgcggatca ccggtaaacc tggcagcggg
    16441 cgaatcacaa tcttcttgcc cggctggttg agtgccgaaa tcggggtaat acaggccaga
    16501 ccattcttgc cgttcatgtt cagaccgtcg gaaccgcaca caccttcacg gcaggagcgg
    16561 cggaacgaca ggctgggatc tttctctttc agctggataa gcgcatccag cagcatcatg
    16621 tcgcgacctt cttccgcttc cagggtgtaa tcctgcatac gcggagcatc atcaacatcc
    16681 gggttatagc gataaattga aaactcgagt ctcattttcc tgtctccgca ttagtaagta
    16741 cgaatcttcg gcgggaatgc cgggcgcagt ttcggttcca tgttgacgct tcggcgcgtc
    16801 atggattccg actctggcag atacagggag tggcacagcc agttttcatc atcacgatcc
    16861 gggaagtcga agcggctatg tgcgccacgg ctttcggtac ggaagttggc agaaacagcc
    16921 gttgcatacg ccgtttccat caggttatcc agttccaggc actcaacgcg ctgggtgttg
    16981 aactcgctgg aagtgtcatc cagacgggca tttttcagac gctcgcggat cactttcaac
    17041 tgctcaagcc ctttcgccat cgcatcacct tcacggaaga ccgagaagtt atgctgcata
    17101 cattcttgca gcgctttacg aatcgccacc ggatcttcac cgttacgatt attgttccag
    17161 cggttcagac gatccagaga tgcttcaaca tcagactcgc tggcatcgcg caatgcgccc
    17221 tgttcggcga tagactcttg cagatgcaga cctgccgcac gaccaaagac caccaggtcc
    17281 agcagcgagt tgccgcccag acggttagcg ccgtgtaccg atacacaagc gatttcacca
    17341 acggcaaaca gccccggaac aaccacatct tcgcctttct cgttcaccgt cagcgcctga
    17401 ccggtcactt tggtcggaat accgcccatc atgtagtgac aggttgggat aaccggaatc
    17461 ggctctttca ccggatcgac gtgagcaaag gtacgggaaa gctccaggat acccggcaga
    17521 cgggattcga gaacttcttt acccaggtga tcgagtttca gtttcgcgtg tggcccccac
    17581 ggaccatcac agccgcgacc ttcacggatt tcaatcatga tggaacgcgc caccacatca
    17641 cggcccgcca ggtctttggc gttcggcgca taacgctcca taaagcgttc gccatgtttg
    17701 ttcagcagat aaccgccttc accacggcaa ccttcggtga ccagtacgcc cgcaccggca
    17761 atgccggtcg ggtggaattg ccacatttcc atatcctgca ccggtacgcc tgcgcggata
    17821 gccatgccga caccgtcgcc agtgttaatg tgggcgttag tggtggactg ataaatacgc
    17881 cctgccccgc cagtcgccag cacggtagcg cgggctttga aataaaccac ttcaccggtt
    17941 tcgatgcaca gtgcggtaca acccaccacc gcgccatcct ggtttttcac cagatccagc
    18001 gcataccact cggagaaaat ggtggtgtgg tttttcaggt tctgctgata aagcgtgtgc
    18061 aacagtgcgt gaccggtacg gtcagccgcc gccgcagtgc gtgccgcctg ctcgccgccg
    18121 aagtttttcg actgaccgcc aaacggacgt tgatagatac gaccatcatc aagacgcgag
    18181 aacggcaggc ccatgtgttc gagttccaga atcgcttccg gcccggtttt acacatatat
    18241 tcaatcgcgt cctggtcacc gatatagtcc gaccctttta cggtgtcgta catatgccat
    18301 tcccagttat cttcatgggt attacccagc gcaacggtaa tgccgccttg cgcagaaacg
    18361 gtatgggaac gggtcgggaa gactttagag agcagcgcac aggtctggcc gctctgggaa
    18421 atttgcagcg ccgcgcgcat acctgcgcca ccggcaccaa tcacaactgc atcaaattct
    18481 ctgactggca atttcatcac acaccccaca ccacaacgaa tccataaatc acgtaaacca
    18541 ccagtgcaac gacaatcacc agttgcagca tcaggcgcaa tgccagcggt ttaacgtagt
    18601 cggtcaacac ctgccacatg ccgatccagg catgaatcaa gatagaaaac agcgccagca
    18661 gggtgaacac tttggtgaac gcagaggcga agaaaccgat ccagacttca tatgtcagtt
    18721 cgccactggt ggcgaaaaaa ccgaccatat aaatgatgta gagcgtcagg acgatagcgg
    18781 tagcgcgaac gaggatgaaa tcatgtacgc cattgcgtcc taatgcggag gcgttgctta
    18841 ccatacgagg actcctgcga gaagtgaaag cacgacagta ataacaaagg agattttggc
    18901 ggagcgttta cccgcttcga atgtttcttc cagatagcca aaatccatca tcatgtggcg
    18961 aatacctacg acgacgtgat acgccagagc ggtaaggatg ccccacatga taaatttgac
    19021 gaagaagctg cccataatcg cggaagcttg ctcgaaacct tcaggggaag agaggctggt
    19081 acccagaagc cacagcagga tgcccactgc aacaaaggtg atcacaccgg aaacgcgatg
    19141 gagaatggac gctatcgccg tgatggggaa ccggatggtc tgtaggtcca gattaacagg
    19201 tctttgtttt ttcacatttc ttatcatgaa taacgcccac atgctgttct tattattccc
    19261 tggggactac gggcacagag gttaactttc tgttacctgg agacgtcggg atttccttcc
    19321 tccggtctgc ttgcgggtca gacagcgtcc tttctataac tgcgcgtcat gcaaaacact
    19381 gcttccagat gcgaaaacga cacgttacaa cgctgggtgg ctcgggattg cagggtgttc
    19441 cggagacctg gcggcagtat aggctgttca caaaatcatt acaattaacc tacatatagt
    19501 ttgtcgggtt ttatcctgaa cagtgatcca ggtcacgata acaacattta tttaattttt
    19561 aatcatctaa tttgacaatc attcaacaaa gttgttacaa acattgccag gaaaagcata
    19621 taatgcgtaa aagttatgaa gtcggtattt cacctaagat taacttatgt aacagtgtgg
    19681 aagtattgac caattcattc gggacagtta ttagtggtag acaagtttaa taattcggat
    19741 tgctaagtac ttgattcgcc atttattcgt catcaatgga tcctttacct gcaagcgccc
    19801 agagctctgt acccaggttt tcccctcttt cacagagcgg cgagccaaat aacaaacggg
    19861 taaagccagg ttgatgtgcg aaggcaaatt taagttccgg cagtcttacg caataaggcg
    19921 ctaaggagac cttaaatggc tgatacaaaa gcaaaactca ccctcaacgg ggacacagct
    19981 gttgaactgg atgtgctgaa aggcacgctg ggtcaagatg ttattgatat ccgtactctc
    20041 ggttcaaaag gtgtgttcac ctttgaccca ggcttcactt caaccgcatc ctgcgaatct
    20101 aaaattactt ttattgatgg tgatgaaggt attttgctgc accgcggttt cccgatcgat
    20161 cagctggcga ccgattctaa ctacctggaa gtttgttaca tcctgctgaa tggtgaaaaa
    20221 ccgactcagg aacagtatga cgaatttaaa actacggtga cccgtcatac catgatccac
    20281 gagcagatta cccgtctgtt ccatgctttc cgtcgcgact cgcatccaat ggcagtcatg
    20341 tgtggtatta ccggcgcgct ggcggcgttc tatcacgact cgctggatgt taacaatcct
    20401 cgtcaccgtg aaattgccgc gttccgcctg ctgtcgaaaa tgccgaccat ggccgcgatg
    20461 tgttacaagt attccattgg tcagccattt gtttatccgc gcaacgatct ctcctatgcc
    20521 ggtaacttcc tgaatatgat gttctccacg ccgtgcgaac cgtatgaagt taatccgatt
    20581 ctggaacgtg ctatggaccg tattctgatc ctgcacgctg accatgaaca gaacgcctct
    20641 acctccaccg tgcgtaccgc tggctcttcg ggtgcgaacc cgtttgcctg tatcgcagca
    20701 ggtattgctt cactgtgggg acctgcgcac ggtggtgcta acgaagcggc gctgaaaatg
    20761 ctggaagaaa ttagctccgt taaacacatt ccggaatttg ttcgtcgtgc gaaagataaa
    20821 aatgattctt tccgcctgat gggcttcggt caccgcgtgt acaaaaatta cgacccgcgc
    20881 gccaccgtaa tgcgtgaaac ctgccatgaa gttctgaaag agctgggcac caaagatgac
    20941 ctgctggaag tggctatgga gctggaaaac atcgcgctga acgacccgta ctttatcgag
    21001 aagaaactgt acccgaacgt cgatttctac tctggtatca tcctgaaagc gatgggtatt
    21061 ccgtcttcca tgttcaccgt cattttcgca atggcacgta ccgttggctg gatcgcccac
    21121 tggagcgaaa tgcacagtga cggtatgaag attgcccgtc cgcgtcagct gtatacagga
    21181 tatgaaaaac gcgactttaa aagcgatatc aagcgttaat ggttgattgc taagttgtaa
    21241 atattttaac ccgccgttca aatggcgggt taatttttat atgcccaaac acaaaaaatt
    21301 gtaaaaataa aatccactat cggacattca tatattctaa aaaaaatata tcggtataaa
    21361 ttataaacaa atcgatgatt tttaatcatc tatcttcctc tcagttacat gaataaacca
    21421 ccaaatatag cgcaccccta taatttccaa tactttattc atcaaacatt taattcatac
    21481 catcaattaa ttacttttac atttaataaa attaataata tttttaagaa aaactaaaaa
    21541 cattcgacaa tcaaataaga tattcttgca ccgatatact tttttctttt ctgttaaagg
    21601 actcagttat gtttaaagga caaaaaacat tggccgcact ggccgtatct ctgctgttca
    21661 cagcacctgt ttatgcggct gatgaaggat ccggtgaaat tcactttaaa ggtgaagtta
    21721 ttgaagcacc gtgtgaaata catcaggatg atattgataa agaggttgaa ctcggtcagg
    21781 tgaccaccag ccacattaat cagtcacatc acagcgatgc cgttgctgtc gacctgcgct
    21841 tagtcaactg tgatctggaa aactccagca acggttccgg tggcaagatt tctaaagttg
    21901 cagtcacctt tgatagctca gcgaaaacca ccggcgcaga tccgattctc aacaacacca
    21961 gcacgggtga agccaccggc gtcggcgtac gtttaatgaa taaagatcaa agcaatatcg
    22021 ttttaggcac cgctactcca gatatcgacc tggctccgac ctccagcgaa caaacgctga
    22081 atttctttgc ctggatggaa caaattgatc aggctacacc tgtaacgcca ggcgcagtta
    22141 cggcgaatgc aacctacgtg ctggattata aataagtatt actttggcgg cgctatcgcc
    22201 gcctttcata aaatatttat caggtatgga caccgtgaat atttatcgac tctcttttgt
    22261 atcctgcctg gtcgtggcga tgccttgcgc attggcggtc gaattcaacc ttaatgttct
    22321 cgataaatcg atgcgcgacc gcattgatat ttcattatta aaggaaaaag gagtcattgc
    22381 tcccggtgag tattttgtta gcgttgcggt aaataataac caaatcagta acgggcaaaa
    22441 gattaactgg cacaaaaatg acgataaaac cattccgtgc atcaatgatt tactggtcga
    22501 taaatttggc ttaaaacctg aagtccgtca gtcgttacca ttgataaatc agtgcgtcga
    22561 ttttagctcc cgacctgaaa tgctcttcaa tttcgatcaa gccaatcagc aactgaatat
    22621 tagtattccg caagcctggc tggcgtggca ctcagaaaac tggcccccac cctccacatg
    22681 gaaagaaggt gtcgccggta tcctgatgga ttacaacttg tttgccagca gctaccgccc
    22741 acaggacggc agcagcagca ctaacctgaa cgcctacggt accaccggaa ttaacgccgg
    22801 ggcatggcgc ttacgtagtg attatcagtt gaatcagact gatagcgatg ataaccatga
    22861 acagtcaggc ggaatatcgc gcacctatct ttttcgtcca ttaccgcaat taggctctaa
    22921 attaaccctc ggcgaaacgg attttagttc caatattttc gacggttttt cttataccgg
    22981 cgcggcactg gcaagtgacg agcgaatgtt gccatgggaa ctacgcggct acgccccaca
    23041 aattagcggt attgcacaga ccaatgccac ggtgacgatc agtcaatcag gccgcgtcat
    23101 ttaccagaaa aaagtcccac caggcccatt tatcattgac gaccttaatc agtctgttca
    23161 gggcacactg gatgtcaaag tgacggaaga agatggtcgg gtgaacaatt tccaggtttc
    23221 ggcagcatcg acgcccttcc tgactcgtca gggacaggtt cgctataaac tggccgcggg
    23281 tcagccacgg ccctccatgt cacatcaaac tgaaaatgaa acctttttta gcaatgaagt
    23341 ttcctggggg atgctgtcaa acacctcgct gtacggcggc ctgctgcttt ctggtgatga
    23401 ctaccattct gccgcaatgg gtattgggca aaatatgctg tggcttggtg cgctgtcgtt
    23461 tgatgtcacg tgggccagta gccattttga tactcagcag gacgagcggg gcttaagcta
    23521 ccgttttaat tacagcaaac aagtggatgc cactaacagc acgatttccc tcgccgctta
    23581 tcgtttctcc gatcgtcatt ttcacagcta cgccaactat ctggatcaca aatacaacga
    23641 cagcgatgcg caggacgaaa aacagacgat cagcttattg tgggccaacc gattacccca
    23701 ctaaacctca atctttacgc caacctgcta catcaaacct ggtggaatgc agacgcctcc
    23761 acgaccgcca acatcacggc aggttttaat gttgatattg gtgactggag agatatctct
    23821 atttcgacgt cattcaatac aacccattac gaagataaag atcacgacaa ccagatttac
    23881 ctgtcgattt cgctcccctt cggtaacggt ggtcgggttg gctatgacat gcaaaacagt
    23941 agccacagca ccacacaccg catgtcgtgg aacgatacgc tggatgaacg taatagctgg
    24001 ggcatgtctg ccggactgca atccgaccgt cctgacaatg gagcccaggt gagcggtaac
    24061 tatcagcacc tgagttcagc gggtgagtgg gatatttctg gtacctatgc cgccaatgat
    24121 tacagttccg tcagcagcag ctggagcggt tctttcaccg caacccaata tggtgcagcg
    24181 tttcatcgcc gcagctccac caatgaacca cgcctgatgg tcagcaccga tggcgtggca
    24241 gatattccgg ttcagggcaa tctcgactac accaaccatt ttggcattgc ggtggtgccg
    24301 ttgatttcca gttaccagcc ttccaccgtg gcggtgaaca tgaatgactt acccgacggc
    24361 gtaacagttg cagaaaacgt tatcaaagaa acgtggattg aaggcgcgat aggttacaaa
    24421 tcactggctt cccgttccgg taaagacgtt aacgtcatca ttcgcaacgc cagcggtcag
    24481 tttcctcccc tcggagcgga tatccgccag gatgacagcg gcattagcgt ggggatggtt
    24541 ggcgaggaag gacatgcctg gttaagcgga gtcgctgaaa atcaaaagtt taccgtggtc
    24601 tggggtgata gccagcattg ctcgctccat cttcctgaac atatggaaga caccgcaaat
    24661 cgcctgattt taccttgtca ttaattaagg aaatgaaaat gacatttatg aaaggactac
    24721 cccttttgtt gttggttgcc agtctgtgca gccacgctgc actacaaccc gatcgcactc
    24781 gtattgtgtt taacgctaat gataaagcta ccagtctgcg ggtggataat cgtagcgata
    24841 aactccccta cctcgcttat tcctggctcg aaaatgaaaa aggggagaaa agcgatgatc
    24901 ttctggtcgc cctgccgccg atacaacgtc tggaaccgaa agcaacaacg caggtacgga
    24961 tagtaaagca agcctcgacc acaaaattac cgggcgatcg cgaaacgcta tttttctaca
    25021 atatgcgtga aattcctccc gccccggaaa aaaacagcga ccatgccgtt ctgcaagtcg
    25081 ccattcagag tcgtatcaag gtgttctggc gaccggctgc attacgcaag aaagcaggag
    25141 aaaaggtcga actgcagtta caggtcagcc agcagggcaa ccaactgaca ctgaaaaatc
    25201 ctaccgcgta ttatctgacc attgcctacc tgggacgaaa tgaaaaaggc gttctccccg
    25261 gcttcaaaac cgtgatggtt gcgcctttta gtacggtcaa cacaaacacc ggaaattata
    25321 gcggcagcca gttttatctc ggttatatgg atgattacgg cgcgttacgc atgacgacgc
    25381 tcaactgcag cggacaatgc catttacaag ccgtggaggc gaagaaatga gtgctggcaa
    25441 gggagtgttg ctcgtcatct gtctgttatt tctgccgttg aagtctgcgc ttgccctgaa
    25501 ctgctacttt ggttcatccg gtggctcggt agaaaaatca gaagcgattc aaccttttgc
    25561 tgtaccaggc aatgccaaac tcggcgataa gatttgggaa tctgacgata ttaaaattcc
    25621 cgtctattgt gacaacaata ccaacggcaa ttttgaaagt gagcatgttt atgcctgggt
    25681 aaccccctac ccgggggtgc aggatcgcta ttatcaactc ggcgtaactt acaatggcgt
    25741 tgattatgac gccaaccagg gaaaaagccg catcgacact aaccagtgta ttgacagtaa
    25801 gaacattgac atttataccc ctgagcagat tatcgcgatg ggatggcaga acaaaatttg
    25861 ctcgggtgat cccgctaata ttcacatgtc gcgtaccttt ctcgctcgca tgcggttata
    25921 cgtcaaaata cgagaaatgc cgccgcatga ttatcaaagt acgcttagcg actatatcgt
    25981 cgtgcaattt gacggtgccg gtagcgttaa cgaagaccct actgcccaaa acctgaaata
    26041 tcatattact ggtctggaaa acattcgcgt gctggattgc agcgtcaatt tttccatttc
    26101 accggaaaca caggtgattg attttggtaa atttaatttg ctggatattc gtcgccacac
    26161 aatgtcgaaa acattcagta ttaagacgac aaaatcacaa aatgaccaat gcaccgacgg
    26221 atttaaagtc agttcctcct tttataccga agaaacgttg gttgaagaag ataaagcatt
    26281 gttgattggt aatggtctga agctacgttt gctggatgaa aacgcctcgc cttatacctt
    26341 caataaatat gctgaatacg ccgatttcac cagcgacatg ttggtctacg aaaaaaccta
    26401 tacagctgaa ctttcgtcca ttgcaggtac ccccatcgag gctgggccct tcgatacagt
    26461 ggtgcttttt aaaattaact ataactgatt tgccatgatg ccctttgcag ccggaaaggg
    26521 catctctttc agaaaattgc tgattttccc cttttccttg attgctgtta ttttttcctg
    26581 gcccggcttt ctttatgctg tatcgataca ccaaagagaa aataatgagg gagcgaagga
    26641 tgccagtttt gcagtgggga atgttatgtg tgctatcact tctcctttct attggtttcc
    26701 tcgcggtaca tctcccggcg gcgctattac ttgggccgat gatcgccggg atcatcttca
    26761 gtatgcgcgg aattactctg caacttcccc gctccgcttt tctcgccgcg caggccattc
    26821 ttggctgcat gattgcgcaa aacctcactg gttcgattct caccacgctg gccgccaact
    26881 ggccgatcgt gcttgctatt ttactggtga cgctgctttc cagcgccatc gtgggttggt
    26941 tattggtgcg ctatagctca ctgcctggaa ataccggtgc ctggggttct tctccaggcg
    27001 gcgcagcggc aatggtcgct atggcgcaag attacggcgc agatattcgc ctggttgcat
    27061 ttatgcaata tctgcgagtg ctgttcgtcg cgggggccgc tgttctggtc acccgcatga
    27121 tgctgggtga taacgctgaa gcggttaacc agcaaatcgt ctggttcccg ccagtgagca
    27181 ttaatctcct gcttacgatt ttgctcgcag tcgttgccgg tacggcggga tgcatgctgc
    27241 gtcttccttc cggcacgatg ctcatcccga tgctggcggg cgcagtgctc cagtctggtc
    27301 agctcatcac catcgaacta ccggaatggc tgctggcgat ggcgtatatg gcaattggct
    27361 ggcggattgg tcttggtttc gataaacaaa tcttactgcg ggcactacgc ccgctaccgc
    27421 aaatcctgct gtcgattttt gctctgctgg ctatttgtgc gggtatggcg tgggggctga
    27481 cccggtttat gcacattgat tttatgactg cctacctcgc caccagtccc ggcgggcttg
    27541 ataccgtggc ggtcatcgcc gcagggagca atgccgatat ggcgcttatt atggcgatgc
    27601 aaaccctgcg cctgttcagt attttgctga cggggccagc cattgcgcgg tttatttcaa
    27661 cctatgcgcc gaagcggtcg gcctagtgct ggcagccagg gcaccagtaa aacgggcgag
    27721 atgacagcgt ggttttctca atgatgctgc cacagcgttc gcacggttcg ccatcacgat
    27781 gaaaaacctt aaagcgaaac agcgccccat gatgcttatt ctcatccacc tgccccctcg
    27841 tagcgtagga aaatcgagga atatccagta acgcgtgtgc gagtgcatcc agttgcgccg
    27901 cattgagatc tttcgcttta tgatttccag tcaacccaac ctgccagagg atctccaccc
    27961 gcaaataatt gccaagccca gccagaaacg cctgatcgag cagtaatcct gcaaactgac
    28021 ggttacgaaa gcgcggtgac aataaccgtt cctttaccac ctccggcgtc agattcggat
    28081 ccagcacatc gggaccaacg cgttgtaaaa acggatgcgt ggtcagttgt tccggcctca
    28141 acatctcaat atccgaggcg ctataaagca gaatggtttt gtcagccgtt tgcagtttta
    28201 cccgcaatac tcgcgtggtc tgcggctctt cgccggtatc aaccacgcgc cagacgccgt
    28261 aaagctgatt atggctgtag agcgttaagt cgttggaaaa atgagttaac aacgccttac
    28321 cacgcgtttc cacatgggta acgtgttgac cgataagttg tgattgataa gtttttaact
    28381 gcgggaaggc aaaccagaca tcagttagtg gtttgccttt gatcgccgcc tccaggttat
    28441 ccgctgcacg gcggatctcc gggccttcag gcatggtgat atcctttttt cattctttat
    28501 taatcggggt tatgctgcga caacaatctc cttttcggca aatgtagagc gtagtcggcg
    28561 ggcgaacgcc agtgcgtgct cgccgtcgcc atgcaggcag actgtttgcg ccgtgaccgt
    28621 tgcccattcg ccggtgatac ttttgactct gccgtgttgc accatctcta gcgtttgcgc
    28681 caacgcctgt tcttcgtttt caatcaacgc gcctcgctga cttcgcggca ccagcgagcc
    28741 gtcagcctga taaccgcgat cggcaaacac ttcctcgcgc gttgtcaggc catattgctt
    28801 gcctgcacga atcagctcgc ttcctgccag cccaacgaga atcaatgccg gatcgcaagc
    28861 gtataccgct ctggcgatgg cgtctgccag ttgtgcttct ttcgccgcct ggttgtacaa
    28921 catgccgtgc ggtttgacat gacgcattac gccgccttgc gcacgggcaa tcgttgccag
    28981 cgcgccaatt tgatacagcg tctgggcgta aacggtttct ggcggcagct gcatggcgct
    29041 gcgaccaaaa ttttccctgt cgggaaaact cgggtgagcg ccaatcgcga caccattttt
    29101 tattgcttca cgcacgcaag cctgcatgat ttgcgcatcg cctgcatgaa atccacaggc
    29161 aatattggca gaggaaacca gcgttaatag ctctgcgtcg ctggcgcagc cttcgcccag
    29221 atcggcgttc aggtcaattt tcattgtgca gccgccacac taattgttcg aaataacgtt
    29281 gctgatcttg ccgcgctttc agtgcctctt ccagtgaaca ctggacaaaa tgaatcggct
    29341 gaccgagcgg aatttgcgcc agatggtaca tatcagcctc aatgatacag gcaatacgcg
    29401 ggtaaccatc ggtggtctgt gcgtcgttca tcaacacaat cggctgcccg ttatgtggca
    29461 cctgcaccac gcccggtaac aaaccgtgag ataacagttc gcgatcggtg gtgcgtttta
    29521 aaatttgccc ctgtaagcga tagcccatgc ggttactttg cgagctaagc tgccagggcg
    29581 aacgccagaa tgcatcctgc gaggcgcgat cgaactcatg atattccggc cccggcaagg
    29641 cgcgaatgcg gttgccccac agcagctgtt taacgccctg cgcttccata aaatcacgct
    29701 tcgatttgcc aatcgggagt cggtcaccat ccttcagtaa acggccttcc agcccgccaa
    29761 tccccacttt gagatcggtg ctgcatgacc ccattaccgg cggaacatca ataccacccg
    29821 cgaccgccag ataactgcgc atcccgtgct gcgggcgttt aagcgttaaa cgctggcctg
    29881 ctttcatcgg caatcgccag ccggtccaga cggcattatc atccagccgc gcttcgcaac
    29941 cggcacccgt cagagcaaac cacccatcag tttcgaactc aaccgttaac tgaccgagcg
    30001 tgatctccag cgcgggggca ttggcgtcat tacccaccag caggttagca atgcgtaacg
    30061 caggcatatc cagtgcgccg cagtggctga ttcccgactg gcgaaaaccg tgacgaccgc
    30121 catcctgcac agtggtatac atgcccgcac gaataatctt cagcatactc cctccttctg
    30181 cggtacaaag cgcacgctgt ctcccggacg taataagatg ggttcgtcac gcgccggatc
    30241 aaacaggctg agtgaggtat gaccaatcaa ctgccagcca cccggcgttg ccagcggata
    30301 aacaccagtc tgcggcccgc cgatcccgac agaacctgcc ggaacgagca agcgcggttc
    30361 agcgcgccgt ggcgtgtgta gttgttccgg caaactcccg agatacggga agcccggttg
    30421 aaaacctaaa aaccagacca cgtattccac ggaggagtgc aattcaacaa cctgtttttc
    30481 gctaaacccg caatgcgccg cgaccaccgc caaatccggt ccgcctgcac caccgtaaac
    30541 caccggaatt tcaataaagc gagactcagg ttccagggcc tcgctctcct cccaccagcg
    30601 ttgcaaacgc tctatggcat ccagcgccag cgactcagga ttacgcaaaa tcaccgtgat
    30661 attgttcatg ccgggaatgg cttcaaccac attcggcata tccaccagac gctgcgccag
    30721 tcgccagatc cgtttctggc tagccagcgt caccggcggt tccagttcca gcactaccgc
    30781 cgtttcacct atcagataac aacgcgctcg ttgcactttc gatccctctg attattcgtt
    30841 atgcaggatt aggaatatca ataaaggtca catcaagatc ggtattttca ttcagccact
    30901 cgctcaatgc gcgaatacca ccacgttcag tggcatggtg acctgcagca taaaaatgca
    30961 acccctgctc gcgggctgaa tgaatggtct gttcagaaac ttcgccagta ataaaagcat
    31021 ccacgccaaa acgcgcggcg ctatcgataa aactttgccc gccgcccgtg caccaggcga
    31081 cgcgctggac cacctcaggt ccggtatcgc cacaccataa cggcttacgt cccagacgcg
    31141 cttcaatcca ggaggctagt tccagtcccg gcacaggcat ggtcagctct ccccacggca
    31201 ccaacggctc aatttcgccc atgaccgtga tccccagtaa cgccgccagc tgtgcgttat
    31261 tgcccagctc aggatgtacg tcaagcggca aatgccagcc atacaggttg atatcattcg
    31321 ccagcaacgt ttttaaacgg ttacgcttca ttccgcgaat gaccggagac tctcctttcc
    31381 agaagtagcc atgatgcaca atgaccgcat ccgcgcccag acgcactgcc tcatcgagca
    31441 aagcctggct ggcggtgaca ccggtaacaa ttttttgcac cgtctctttg ccttctacct
    31501 gcaaaccatt cggcgcgtaa tcactaatcg ccgcgctgtt cagtttttcg ttaatcagtt
    31561 gttccagttc ggtgtttttc atcttcgcgc cttccgtatt ctgatagcta caccgttaac
    31621 atagcgttac ctcgcctgtt cgtcgatagc caatcttttg acatattaag aaacaaaccc
    31681 gacgccaaaa gacgcaaatt gtttatatca tcacgcttca gctaatgtat atttatgcaa
    31741 tcagacagca tattgcataa ataatcagat ctgtatcaaa gttttaactt taaggaaaca
    31801 caagcacgcc gcgcaactat tacgtgacgt tgcagtcaat tgtggaatta ttatgaataa
    31861 acacgcatca caaccgcgca ctatttacta tgtcgttgcg ctgcaaatct gggaatattt
    31921 tagcttttac ggcatgcgtg ccctgctgat tctctatctc accaatcaac taaaatacaa
    31981 cgatactcac gcctacgagt tatttagcgc ctactgttcg ctggtgtatg tcacgccaat
    32041 cctcggtggc tttttggcgg ataaagttct cggcaatcgc atggcggtga tgctgggggc
    32101 gttgttgatg gcgatcggtc atgtggtgct gggtgccagt gagatccatc cgtcattcct
    32161 ctatctgtcc ctggcgatta tcgtttgtgg ctatggtctg tttaaatcca atgtcagttg
    32221 cctgctcggc gagctgtatg agccaaccga tccgcgtcgt gatggcggtt tctcgctgat
    32281 gtatgcagcg ggtaacgtgg ggtctattat cgcacctatc gcctgtggtt acgcccagga
    32341 agagtacagc tgggcgatgg gctttggcct ggcggcggtg ggtatgatcg cgggtctggt
    32401 cattttctta tgtggcaatc gtcatttcac acatacccgc ggcgttaata aaaaagtact
    32461 gcgtgcaaca aactttctcc tgcctaactg gggatggctg ctggttctgc tggtggcaac
    32521 gcctgcgctg attaccgtac tgttctggaa agagtggtcg gtatacgcct taattgtcgc
    32581 gactattatt ggcctgggtg tactggcaaa aatttatcgc aaagcagaaa accagaaaca
    32641 gcgtaaggag ctggggctga ttgtgacgct caccttcttc agtatgttgt tctgggcctt
    32701 cgcacaacag ggcggcagct caattagcct ttatatcgac cgcttcgtta accgcgatat
    32761 gtttggttat accgttccga ctgctatgtt ccagtcgatt aatgccttcg cagttatgct
    32821 atgcggtgtg ttcctggcgt gggtggttaa agaaagcgtc gcaggtaatc gtaccgtgcg
    32881 catctgggga aaatttgctc ttggccttgg cctgatgagc gccggattct gcattctgac
    32941 cttaagcgcc cgctggtccg caatgtatgg tcactcttct ctgccactga tggtgttagg
    33001 cctcgcggtg atgggctttg cggaactgtt tatcgacccg gttgccatgt cgcaaattac
    33061 gcgcattgaa attccgggcg tgaccggcgt attaaccggc atctacatgc tgctttctgg
    33121 cgcgatcgcg aactatctgg cgggcgtgat tgccgatcag acatcgcagg cttcgtttga
    33181 tgcttccggg gcgatcaact actccatcaa tgcatatatt gaagtatttg atcaaattac
    33241 ctggggcgca ctggcgtgtg taggactggt actgatgatt tggctgtatc aggcgctgaa
    33301 attcagaaac cgcgcgctgg cgctggagtc ataataggcc agcaccgatg cctgatgcga
    33361 cgcttgacgc gtcttatca
//
LOCUS       DAZOZY010000024        32838 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZOZY010000024 DAZOZY010000000
VERSION     DAZOZY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32838
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.024"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHURQ_001009"
     CDS             267..1925
                     /locus_tag="ACHURQ_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR0822850.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHURQ_001010"
     CDS             complement(1922..2878)
                     /locus_tag="ACHURQ_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR0822851.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHURQ_001011"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHURQ_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR0822852.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHURQ_001012"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHURQ_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR0822853.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHURQ_001013"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHURQ_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR0822854.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHURQ_001014"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHURQ_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR0822855.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHURQ_001015"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHURQ_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR0822856.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHURQ_001016"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHURQ_001016"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR0822857.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHURQ_001017"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHURQ_001017"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHURQ_001018"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHURQ_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR0822858.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHURQ_001019"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHURQ_001019"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR0822859.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHURQ_001020"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHURQ_001020"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR0822860.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHURQ_001021"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHURQ_001021"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR0822861.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHURQ_001022"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHURQ_001022"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR0822862.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHURQ_001023"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHURQ_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR0822863.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHURQ_001024"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHURQ_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR0822864.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHURQ_001025"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHURQ_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR0822865.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHURQ_001026"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHURQ_001026"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR0822866.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHURQ_001027"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHURQ_001027"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR0822867.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHURQ_001028"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHURQ_001028"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR0822868.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHURQ_001029"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHURQ_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR0822869.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHURQ_001030"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHURQ_001030"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR0822870.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHURQ_001031"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHURQ_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR0822871.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHURQ_001032"
     CDS             29803..30951
                     /locus_tag="ACHURQ_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0822872.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="ACHURQ_001033"
     CDS             31161..32591
                     /locus_tag="ACHURQ_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0822873.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32592..>32838)
                     /locus_tag="ACHURQ_001034"
     CDS             complement(32592..>32838)
                     /locus_tag="ACHURQ_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0822874.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8058 a         8031 c         8814 g         7935 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcgagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ctgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccaccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctattgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccccaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatca
//
LOCUS       DAZOZY010000025        31117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZOZY010000025 DAZOZY010000000
VERSION     DAZOZY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.025"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            19..714
                     /gene="araD"
                     /locus_tag="ACHURQ_001035"
     CDS             19..714
                     /gene="araD"
                     /locus_tag="ACHURQ_001035"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR0822875.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            789..3140
                     /gene="polB"
                     /locus_tag="ACHURQ_001036"
     CDS             789..3140
                     /gene="polB"
                     /locus_tag="ACHURQ_001036"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR0822876.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3304..6210
                     /gene="rapA"
                     /locus_tag="ACHURQ_001037"
     CDS             3304..6210
                     /gene="rapA"
                     /locus_tag="ACHURQ_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR0822877.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6222..6881
                     /gene="rluA"
                     /locus_tag="ACHURQ_001038"
     CDS             6222..6881
                     /gene="rluA"
                     /locus_tag="ACHURQ_001038"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR0822878.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHURQ_001039"
     CDS             complement(6998..7813)
                     /gene="djlA"
                     /locus_tag="ACHURQ_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR0822879.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8068..10422
                     /gene="lptD"
                     /locus_tag="ACHURQ_001040"
     CDS             8068..10422
                     /gene="lptD"
                     /locus_tag="ACHURQ_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR0822880.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10475..11761
                     /gene="surA"
                     /locus_tag="ACHURQ_001041"
     CDS             10475..11761
                     /gene="surA"
                     /locus_tag="ACHURQ_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR0822881.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHURQ_001042"
     CDS             11761..12750
                     /gene="pdxA"
                     /locus_tag="ACHURQ_001042"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR0822882.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHURQ_001043"
     CDS             12747..13568
                     /gene="rsmA"
                     /locus_tag="ACHURQ_001043"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR0822883.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13571..13948
                     /gene="apaG"
                     /locus_tag="ACHURQ_001044"
     CDS             13571..13948
                     /gene="apaG"
                     /locus_tag="ACHURQ_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR0822884.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            13955..14797
                     /gene="apaH"
                     /locus_tag="ACHURQ_001045"
     CDS             13955..14797
                     /gene="apaH"
                     /locus_tag="ACHURQ_001045"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR0822885.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHURQ_001046"
     CDS             complement(14875..15354)
                     /gene="folA"
                     /locus_tag="ACHURQ_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR0822886.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHURQ_001047"
     CDS             complement(15546..17408)
                     /gene="kefC"
                     /locus_tag="ACHURQ_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFR0822887.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHURQ_001048"
     CDS             complement(17401..17931)
                     /gene="kefF"
                     /locus_tag="ACHURQ_001048"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR0822888.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18039..19370)
                     /locus_tag="ACHURQ_001049"
                     /pseudo
     CDS             complement(18039..19370)
                     /locus_tag="ACHURQ_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHURQ_001050"
     CDS             complement(19429..19716)
                     /gene="fixX"
                     /locus_tag="ACHURQ_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR0822889.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(19713..20999)
                     /locus_tag="ACHURQ_001051"
     CDS             complement(19713..20999)
                     /locus_tag="ACHURQ_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0822890.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMNYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21050..21991)
                     /locus_tag="ACHURQ_001052"
     CDS             complement(21050..21991)
                     /locus_tag="ACHURQ_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0822891.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22006..22776)
                     /locus_tag="ACHURQ_001053"
     CDS             complement(22006..22776)
                     /locus_tag="ACHURQ_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR0822892.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            23249..24763
                     /gene="caiT"
                     /locus_tag="ACHURQ_001054"
     CDS             23249..24763
                     /gene="caiT"
                     /locus_tag="ACHURQ_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR0822893.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            24794..25936
                     /gene="caiA"
                     /locus_tag="ACHURQ_001055"
     CDS             24794..25936
                     /gene="caiA"
                     /locus_tag="ACHURQ_001055"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR0822894.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            26065..27282
                     /gene="caiB"
                     /locus_tag="ACHURQ_001056"
                     /pseudo
     CDS             26065..27282
                     /gene="caiB"
                     /locus_tag="ACHURQ_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            27356..28909
                     /gene="caiC"
                     /locus_tag="ACHURQ_001057"
     CDS             27356..28909
                     /gene="caiC"
                     /locus_tag="ACHURQ_001057"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR0822895.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            29018..29803
                     /gene="caiD"
                     /locus_tag="ACHURQ_001058"
     CDS             29018..29803
                     /gene="caiD"
                     /locus_tag="ACHURQ_001058"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR0822896.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            29809..30399
                     /gene="caiE"
                     /locus_tag="ACHURQ_001059"
     CDS             29809..30399
                     /gene="caiE"
                     /locus_tag="ACHURQ_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR0822897.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="ACHURQ_001060"
     CDS             complement(30608..31003)
                     /gene="caiF"
                     /locus_tag="ACHURQ_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR0822898.1"
                     /translation="MCEGYVEKPPYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
BASE COUNT         7541 a         8375 c         8150 g         7051 t
ORIGIN      
        1 gacctctcag gagtcaacat gttagaagat ctcaaacgcc aggtattaga agccaacctg
       61 gcgctgccaa aacacaacct ggtcacgctc acatggggca acgtcagcgc cgttgatcgc
      121 gagcgcggcg tctttgtgat caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac
      181 gatatggtcg tggttagcat cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc
      241 tccgacacgc caactcaccg gctgctctat caggcattcc cctccattgg cggcattgtg
      301 catacgcact cgcgccacgc caccatctgg gcgcagtcgg gtcagtcgat tccagcaacc
      361 ggcaccaccc acgccgacta tttctacggc accattccct gcacccgcaa aatgaccgac
      421 gcagaaatca acggcgaata tgagtgggaa accggtaacg tcatcgtaga aacctttgaa
      481 aaacagggta tcgatgcagc gcaaatgccc ggcgttctgg tccattccca cggcccgttt
      541 gcatggggca aaaatgccga agatgcggtg cataacgcca tcgtgctgga agaggtcgct
      601 tatatgggga tattctgccg tcagttagcg ccgcagttac cggatatgca gcaaacgctg
      661 ctggataaac actatctgcg taagcatggc gcgaaggcat attacgggca gtaatgactg
      721 tataaaacca cagccaatca aacgaaacca ggctatactc aagcctggtt ttttgatgga
      781 ttttcagcgt ggcgcaggca ggttttatct taacccgaca ctggcgggac accccgcaag
      841 ggacagaagt ctccttctgg ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac
      901 cgcaagagtc cgtggcgttt attcccgccg atcaggttcc ccgcgctcag catattttgc
      961 agggtaaaca aggctttcgc ctgacaccgc tggcgttaaa ggattttcac cgccagccgg
     1021 tgtatggcct ttactgtcgc gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg
     1081 aaggtggcgt taccgtctac gaggccgatg tgcgcccgcc agaacgctat ctgatggagc
     1141 ggtttatcac ctcaccggtg tgggtcgagg gtgatatgca caatggcact atcgttaatg
     1201 cccgtctgaa accgcatccc gactatcgtc cgccgctcaa gtgggtttct atagatattg
     1261 aaaccacccg ccacggtgag ctgtactgca tcggcctgga aggctgcggg cagcgcatcg
     1321 tttatatgct ggggccggag aatggcgacg cctcctcgct tgatttcgaa ctggaatacg
     1381 tcgccagccg cccgcagttg ctggaaaaac tcaacgcctg gtttgccaac tacgatcctg
     1441 atgtgatcat cggttggaac gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg
     1501 agcgttaccg tcttccgctg cgtcttgggc gcgataatag cgagctggag tggcgcgagc
     1561 acggctttaa aaacggcgtc ttttttgccc aggctaaagg tcggctaatt atcgacggta
     1621 tcgaggcgct gaaatccgcg ttctggaatt tctcttcatt ctcgctggaa actgtcgctc
     1681 aggagctatt aggcgaagga aaatctatcg ataacccgtg ggatcgaatg gacgaaattg
     1741 accgccgttt cgccgaagat aaacctgcgc tggcaactta taacctgaaa gattgcgagc
     1801 tggtgacgca gatcttccac aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg
     1861 tgaacggcct gccggtggat cggcacggcg gttcggtggc agcgtttggt catctctatt
     1921 ttccgcgaat gcatcgcgct ggttatgtcg cgcctaatct cggcgaagtg ccgccgcaca
     1981 ccagccctgg cggctacgtg atggattcac ggccagggct ttatgattca gtgctggtgc
     2041 tggactataa aagcctgtac ccgtcgatca tccgcacctt tctgattgat cccgtcgggc
     2101 tggttgaagg catggcgcag cctgatccag agcacagtac cgaaggtttt ctcgatgcct
     2161 ggttctcacg ggagaaacat tgcctgccgg agattgtgac taacatctgg cacgggcgcg
     2221 atgaagccaa acgtcagggt aataaaccac tttcgcaggc gctgaagatc attatgaatg
     2281 ccttttatgg cgtgcttggc accaccgcct gccgcttttt cgatccgcgc ctggcatcgt
     2341 cgatcaccat gcgtggtcat cagatcatgc ggcaaaccaa agcattgatt gaagcgcagg
     2401 gctacgacgt tatctacgga gataccgact cgacttttgt ctggctaaaa ggtgcacatt
     2461 cggaagaaga ggcggcgaaa atcggtcgtg cactggtgca gcacgttaac gcctggtggg
     2521 cggaaacgct gcaaaaacaa cggctgacca gcgcattaga actggagtat gaaactcatt
     2581 tctgccgttt tcttatgcca accattcgcg gagctgatac cggcagtaaa aagcgctacg
     2641 ccgggctgat tcaggagggc gataaacagc ggatggtgtt taaagggctg gaaaccgtgc
     2701 gcaccgactg gacgccgctg gcccagcagt ttcagcagga gctatacctg cgcatcttcc
     2761 gcaacgagcc atatcaggaa tatgtacgcg aaaccatcga caaactgatg gcgggtgaac
     2821 tggatgcgcg actggtttac cgtaaacgcc ttcgccgtcc gctgagcgag tatcagcgta
     2881 atgtgccgcc tcatgtacgc gccgctcgcc ttgccgatga agaaaaccaa aagcgtggtc
     2941 gccccttgca atatcagaat cgcggcacca ttaagtacgt atggaccacc aacggcccgg
     3001 agccgctgga ctaccaacgt tcaccactgg attacgaaca ctatctgacc cgccagctac
     3061 aacccgtggc ggagggaata ctccctttta ttgaggataa ttttgctaca cttatgaccg
     3121 ggcaacttgg gctattttga gcaaaaaaag agttcgccag ataccatttt gatgcgtgac
     3181 gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga ataacaccac
     3241 taccccataa gcacggtagc gggtggttat tgcctgcaat taaagatata gagccgaaca
     3301 catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga attgggactt
     3361 ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc tactggtgaa
     3421 aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa ccctggtgat
     3481 actattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga agaaaatggc
     3541 ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa
     3601 gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag
     3661 attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag
     3721 ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc gcatcagctc
     3781 aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg
     3841 ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct ctctggcgct
     3901 gctgaacgtg tgctaattat cgtcccggaa accttacagc atcagtggct ggtagaaatg
     3961 ctgcgccgtt tcaacctgcg ctttgcgcta tttgatgatg agcgttatgc cgaagctcag
     4021 cacgatgctt acaacccgtt cgacaccgaa cagctggtga tttgctcgct ggattttgcc
     4081 cgtcgtagca aacagcgcct ggaacatctc tgtgaagccg aatgggacct gctggtggtc
     4141 gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt
     4201 gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg
     4261 gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt
     4321 gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt tgccatgctg
     4381 ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat gatcggcgag
     4441 caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc
     4501 cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt gctgttccgt
     4561 aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat taagctgccg
     4621 ctgccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc acgtaaaagt
     4681 gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga atttgaaggt
     4741 gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg ctacctgacc
     4801 agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc gctgcaactg
     4861 gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg
     4921 attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg cgcacaggta
     4981 ctgctgtgct cagaaatcgg ttctgaagga cgtaacttcc agttcgccag ccacatggtg
     5041 atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg tctggatcgt
     5101 atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac cgctcagtcg
     5161 gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg cccgaccgga
     5221 cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc accggttcaa
     5281 accgaaggct ttgacgatct gatcaaaaac tgccgcgagc aacatgaagc gctgaaagca
     5341 cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc
     5401 caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat cgccttcgcc
     5461 atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa catgatcgtg
     5521 ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga agatggcatc
     5581 accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt tattacctgg
     5641 gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac tggtagcagc
     5701 acgatttcac tattgaaaaa caaagcgttg ccggtaggta cgctgttggt ggaactgatt
     5761 tatgtcgttg aagcccaggc tccgaagcag ttgcagctca accgcttcct gccaccgacg
     5821 ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt agagtttgaa
     5881 acctttaacc gtcagcttaa cgcggttaac cgtcacaccg gcagcaaact ggttaacgcc
     5941 gtgcagcagg atgttcacgc tatccttcag ctgggtgaag cgcagatcga gaaatctgcc
     6001 cgtgcattga ttgatgccgc acgtaacgaa gccgacgaaa aactgtctgc cgagctgtct
     6061 cgtctggaag cgctgcgcgc agtgaacccg aacattcgtg acgacgaact gaccgccatt
     6121 gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggctggcg tctggatgcc
     6181 ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg gaaaactaca
     6241 atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccacatt atggtggtta
     6301 acaagccgag cggtttgttg tctgtgccgg ggcgtctgga agagcacaaa gacagcgtga
     6361 tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg gatatggcta
     6421 ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta aaacgccagt
     6481 tccgcgagcg cgagccgaaa aagcagtatg tagcccgcgt ctgggggcat ccatcccctg
     6541 cggaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg aaacagaaag
     6601 tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag tatgcggcgg
     6661 ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcag ctgcgtgtgc
     6721 atatgctggc gctgggtcat ccgattctcg gcgatcgttt ttatgcatca ccagaagcga
     6781 gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc catccggcgt
     6841 atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc tggcgcagtt
     6901 cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg
     6961 acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct ttctgctgct
     7021 ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct
     7081 caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc agcttacggt
     7141 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
     7201 cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat
     7261 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgggcgc
     7321 gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
     7381 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
     7441 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttatctgatt
     7501 ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
     7561 ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg gatttggtta
     7621 aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
     7681 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccta
     7741 acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca atgatttttc
     7801 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
     7861 taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc tataatttgc
     7921 attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc ggacaggatt
     7981 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
     8041 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
     8101 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
     8161 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
     8221 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
     8281 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
     8341 aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg taatgtccat
     8401 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
     8461 gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg cggtaaagcg
     8521 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
     8581 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
     8641 gaagaacaag ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
     8701 tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
     8761 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
     8821 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
     8881 tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga actggactat
     8941 ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc acgtcgttgg
     9001 ttgttctact ggcaacactc cggggtcatg gatcaagtgt ggcgtttcaa cgtcgactac
     9061 accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg ttccagtact
     9121 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
     9181 gtttcaacca agcagttcca ggtctttagc gagcagaaca ccagtagcta ctcggcagag
     9241 ccgcagttag acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
     9301 ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac ccgtgttcac
     9361 ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa taccgaagcg
     9421 aagttgctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
     9481 accaagctgg acgaatccgt taaccgcgtt atgccgcaat tcaaagttga cggcaaaatg
     9541 gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct ggaaccgcgc
     9601 gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta cgactcgtct
     9661 ctactgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg tcttgaccgt
     9721 attgcctccg ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
     9781 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
     9841 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg ggcaggcgat
     9901 acttactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta cgatacacgt
     9961 ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg
    10021 gtacagctga attaccgtta cgccagcccg gaatatattc aggctacgct gcctaagtac
    10081 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt cgccagctgg
    10141 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
    10201 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt
    10261 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
    10321 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
    10381 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
    10441 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
    10501 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
    10561 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
    10621 aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc tgcgccacca
    10681 aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt
    10741 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
    10801 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg
    10861 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
    10921 catcaccatc ctgccgcagg aagtcgaatc cctggcgcag caggtgggta accaaaacga
    10981 cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa acccgacttc
    11041 tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg
    11101 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
    11161 ccagatgggc tggggccgta ttcaggagtt gccgggtatc ttcgcccagg cattaagcac
    11221 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
    11281 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
    11341 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgtgtga aactggaaca
    11401 gattgctgcc gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
    11461 tcaggatcca ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
    11521 cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
    11581 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
    11641 cgacgctgcg cagaaggatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
    11701 agcagcaagc tggatgcagg aacaacgcgc cagcgcctac gttaaaatcc tgagcaacta
    11761 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
    11821 ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc
    11881 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
    11941 cccaactccc ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta
    12001 cgcgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt ggtggagacg
    12061 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
    12121 gtgcataaag gcgtcattaa cgacgcaggt attccgttta ctggtcatac cgagtttttc
    12181 gaagagcgct cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
    12241 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgcactt
    12301 ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg tattgccgaa
    12361 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
    12421 gaagagatag acaccattat tccggtgctc aacgagctgc gggcgcaggg gatgaaactc
    12481 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
    12541 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
    12601 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
    12661 cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc gcttaatctc
    12721 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
    12781 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
    12841 ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg gcggcattga
    12901 ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
    12961 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
    13021 cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg cgtgttttcg
    13081 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
    13141 ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac
    13201 cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
    13261 cagtgctgga agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
    13321 tgcgcctggt tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc
    13381 gcatcaccac tgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
    13441 tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata
    13501 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
    13561 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
    13621 ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
    13681 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
    13741 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
    13801 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
    13861 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
    13921 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
    13981 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa
    14041 agataccctc tggctgacgg gcgatctggt cgcgcgcggg ccgggttcgc tggatgttct
    14101 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc acgatctgca
    14161 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
    14221 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctttgctgca
    14281 aatcgacgaa gagaaaaagt tggtgatggc ccacgcaggg atcacgccgc agtgggatct
    14341 acagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctatcgagtg actcctatcc
    14401 cttctttctc gacgctatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
    14461 gctgggaaga ctgcgtttta tcaccaacgc ttttacccgt atgcgttttt gcttcccgaa
    14521 cggtcaactg gatatgtaca gcaaagaatc gccggaagag gcccctgccc cactgaaacc
    14581 gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg gtcactgggc
    14641 atcgctggag ggcaaaggta cgccggaagg tatatacgcg ctggataccg gctgctgctg
    14701 gggtggtaca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
    14761 ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata taggaaggcc
    14821 ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac aaaattaccg
    14881 ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcagcat cgtggaattc
    14941 gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac
    15001 ttctgcgtcg atatgcgtca gatacagttt ctgcgctttt ggcaggaact gctcataaac
    15061 gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcatc
    15121 caccgacttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt
    15181 tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca taatcacggg
    15241 tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc
    15301 gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga tcattgagat
    15361 ttcccgataa aaaaaattgt cgccactata cgtaaagcgt aaaccgtcgt cgactggtgc
    15421 gaggatgatg ttgaggaaaa ttttatattc tgctggcgag tccacgctct ctccctggac
    15481 tcgccgcatt acaatgaaac aaaaacaaac agttagctgt aaagtgtgat ttacgtcact
    15541 ctttattagg atgagggttt cgtttccggt tcatccgcca tgttgccggt atgtttacct
    15601 tcttcggttc cctgccagcc atgtcgttga attaatgaca gatgttcgcg gtcctcggta
    15661 atgatctcac ttaacatcgc gctggtgcgt ttatagaccg ccgcgcgggc tttggtgtcg
    15721 ttctcaacca ttgccatctc ttccaccatc tgaatattaa agcggcggaa cacatcggca
    15781 cgttctcgcg cttcatacgg ccccagacct aaactttcca gtgccagacg cccggttttc
    15841 agcgcacctt cgaaggtttc acgctccggc ttttcaacgc ctgcctgacg caaacgaatg
    15901 tagtggtcga catcgcgggc gcgggcaata atctgcaaat gcgggaagtg ttctttcacc
    15961 atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg cgttaatcag cacttccgct
    16021 ttcgccgctc ccgcagattc cagtaaatcc atccgcgtgg catcgccata aaacactttc
    16081 ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt gatcgagcac caccattttc
    16141 accccgctgg agagcagtaa acgtccggtg atctgcccaa aacgaccgaa tccggcaatg
    16201 attacccgcg gctgttcttc gtcgatctca tcagcttcac gcgcctcttc agtagaagat
    16261 tgctcaaggc gattgaggat caccagcaga atcggcgttg ccgccatcga cagcgccacc
    16321 gccagggtca gcgatttcgc ccactccggt tccagcacat tcgccatctg cgccgtgcca
    16381 aataccacaa aggcaaactc actgccctgc cctaacaaca ccgcaaacca acgacgctgt
    16441 ttatttggca cttgcaacgg tcgggcaatc agccacagca tggcgatttt gatgatgagg
    16501 aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa gcagcgtgcc aaagtctatc
    16561 gacatgccaa caccgatgaa aaacagcccc aacagcaaac ctttaaatgg ttcgatatcg
    16621 ctctccagcg catgacggta ttccgagctt gccagcagta cgcccgccag aaacgcgccc
    16681 atcgccatcg acaagccgac ctcttccagc agcaaaccaa agccaaacac gaggaataac
    16741 gccacggcac taaacacttc ccgcaagcca gagcgggcta caaaacgcag cgccggacgc
    16801 gtgacatagc gccccagcaa taccaccagc accagcgcac ccgccacttt taacgccgag
    16861 agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg ccagtagcgg aatcatcgcc
    16921 accagcggga tcgccgcgat atcctggaac agcagcaccg caaaggcact gcgacccatt
    16981 tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg caatcgccgt agaggagagc
    17041 gccagcgtca tgccgatcaa ttccgcgacc tgccagtgca gcccaagtaa catgcagaac
    17101 agccccagca ggccgccgca aatcaccatc tgcaatgcgc caccgccgaa cactgccgca
    17161 cgcagcttcc acagcctttg tggatcgagt tcgaggccga taataaacag catcagcacc
    17221 accccaatct cggcaaagtg cagaatagat tcggcatcgg tcaccagtcg cagcccccac
    17281 gggccaataa tgcagccggc gatcaggtag ccaagtaccg atcccagacc aagacgtacc
    17341 gcaatgggta caatcagcgc tgccgaaccg agataaatca gcgcctgaat cagcgtatgg
    17401 ctatccatga tgggcctcct gccattccag cagacgttgc ttatagtgac gcgcctgccc
    17461 ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg gcaaacggtg gcagccagtt
    17521 cagcccgcag tagattgccg tcgcctgtag cggctgcgac agcacatcaa agcccggatg
    17581 cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc accgcccaca gcaaatattt
    17641 gccatgcagc gccttgccgc cgtgaccgta agcccagccg tgcgacaaaa ctttatcgat
    17701 ccaaagttta agaagcggag gaatgctgta ccactgcatc ggatgctgcc agacgatcag
    17761 atcggcgcga gacagcgcct cctgctcggc ggcaatatcg atattgaagt caggatagag
    17821 ttgataaaga gagcgaattt cgacgccttc cagcgtcctt gcctgttcaa gcatccgttt
    17881 attcgcatgg gaatgatgcg gatacggatg cgcataaatt ataagaatca taggtagccc
    17941 gcgtgattac gcctttgttt tcccacaaag tgtagtcata tattcacgcg gcttatagtc
    18001 aataatattg attcagaaaa gagagaaatc ttaacaattt accccattct ctgcccgcgg
    18061 atcgtcatat tgctggtttg cgccagtgac atccctcgag tctccggggc aaacgctacg
    18121 gaaatcaaca agccaaacag cgagataccc gcctccatta gcatcgtgtt actgataccg
    18181 taattattga taaagatcgg cagtgcccag gtcgaaacaa tggtgccaat acggcttaag
    18241 gacataatca cgcccacggc agaggcgcgg atatctgtcg ggaagagttc attaggatag
    18301 agccactgca aattacccgg cccgccagag aaaaaggcat acaccgcaaa ggccatcact
    18361 accagccaga tccccatatc cgggatcagc cccaaaaccg ccagcgccag cgtcatcatg
    18421 gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt ttaaccacag catcggcgga
    18481 atacagccga gcataaagaa caggctaatc accacgttcc ccagcgccgc gtttttgcca
    18541 acacccaatc ccaacaaacc aacgatttgc gggccaaagg tgtaaatggc gaacattggg
    18601 atcacctggc aggtccagat ggcggcaaca aacagaacaa aaggaaaatg gcggcgatta
    18661 aacagatcgc gaaaacgggt ttgctacggc tgctcttcat cgaaagccac cggttcgcca
    18721 aacagtttta tcatcatctc ttcgcactct tttactcgcc ctttgcgtaa taaccagcgg
    18781 ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca aacaggggat cgccgcgcta
    18841 cccagcatcc agcgccagcc gccttccaca tcataaagcc agtagccgac cagatcggcg
    18901 caggtcgcgc cgacatacca catggcggcg ataaagctga tggaaaatgc ccgctgacgg
    18961 gtactggaga actcggtgat cattgaggtg gcgatgggat aatctgcacc gatgacaatg
    19021 ccgataagta cccgcatcac caacagttcg acgggggatg aaacaaacat cgtcgccacc
    19081 gatatcacgc cgatggcgat gatatcaatg aggaacattt tgcgccgtcc tactttatcg
    19141 gaaatatagc caaacagcga tgtgccaacg aacagcccgg cgagcgttcc cgcgcccaac
    19201 aagccaatcc agtcagcgtc cagttttagc gccggagtca gttgctccag cgccacgcca
    19261 atcattacca gtacataacc atccagaaac ggaccaccgc ttccccacag caaaatgcgg
    19321 cggtgaattg aggagaattt gagatcgtca aagtttctgg acggttgcat gggttattcc
    19381 tgtttttttg caatgagagt cagtacggcg ttacgtatca aaccaacatc agccgtaacg
    19441 gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt tccagcgccg accccagccc
    19501 cagaatgcga caggtgccgc actccagaca tccggcgtaa tcgaagcgca cactgccgtc
    19561 atcctgcttc ttgtacagac ctgcggggca cgctttcacc agcagctcca gggcctgttt
    19621 atcggcatcg gccttcacaa caatgtgcgg atgctcttca tcgacattga atttattgac
    19681 gcccagtttg acgtccacat tgacgggaga agtcataaaa cggtcactcc tttcatgcca
    19741 tccttgatca gattgatgaa gcccactttc ttgccgtggc ggaggatttt cttgcgcatc
    19801 agttccggcg cactgccgtc aatggtgaac aggtcacgcg ccacgcccac cgccagttcc
    19861 gggtagccgc taaacatgcg tgggttatca aggaaggccg gtagtttctg gtacatacgc
    19921 atatcgcgca gcgggccact ctcaagatgc tgacgatatt ccgccagttt ttgcttactg
    19981 aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg cggcttcccc ggcggcaatc
    20041 gccagatcca taccgcgaat ggtaaaaccg aggttcatac acattccggc ggcatcaccg
    20101 gcaatcaata cgccgtcacc aaccaactcc ggcagcatgt tgatgcctgc ttccggcact
    20161 acgtgagcgg aatattccac cagcttgccg cccgcgatca gcggtgccac tgccggatgc
    20221 tgtttgaaat cttccagcat ttgcggcacc gatttttttg cgtcatgcag atgatgcaga
    20281 ccacaaacca gccccagcga cagggtattt tcattggtat aaaggaagcc gccgcccatc
    20341 aggccatcgg tgggtgatcc cgcaaacaga caagccgcgc cctgattacc ctgcaactga
    20401 aaacggtctt cgattaccga cttcggtaac tcgatcagtt ccttcacgcc aaccgccaca
    20461 tccgtcggtt taacgcgttt tgccatcccc agcttttcgg caaggatgga gttcactcca
    20521 tcagcaagga tcaccgtttt cgcttcaatc acatcgccat cggcttctac accgacgact
    20581 ttgccatcgc gctgtacgag gttatcgacg cggatcccgg taattaactg cgcgcccgct
    20641 tcttcggcct gctccatcag ccaggcatca aatttactgc gcaaaacgga gtaagaacgc
    20701 tgggatggcg aggtttcgtc accgttgcag tagttcatag tcatcgctga cttttccgtc
    20761 ataaacgcga gtttttcatg ggtgatcagg cgttctaccg gggcggattc ggcgaaacca
    20821 ggaataatgt gttccaggct gtgggcatag agacgcccgc cggtgacgtt cttggcacct
    20881 gcggaattgc cacgctcgat aactaacact tgcgcccctt cgcgggcgag caccagtgcg
    20941 gcaaccgaac cggcaagccc agcaccgacg atgatggcgt caaagatatc ttcggacata
    21001 agagctccct gtcagcggca gggccaaaat gcagccctgc cagagtggat caacgcgcta
    21061 aagctgcggt cagcgccgga aggatcttca cggcgtcacc aacaatgccg taatccgcgt
    21121 actggaagat cggcgcattt ttatctttat tgatggcgaa aatggtttgc gatgcattag
    21181 caccaaccat gtgctggatc tgcccggaga tccccaccgc caggtacagt tcaggtttca
    21241 gcatcaggtt ggagataccg acatagcgtt cgtgctccat ccatttttcg ttttccgcca
    21301 ccggacgaga acaggccaac tccgcaccta tcgccttgca aagctgttct gccagcgcaa
    21361 tgttctcttt gctgccaata ccgcgaccga cgctgaccac cagacgggct ttgtcgagat
    21421 cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt aatcgccaca gccggagcct
    21481 gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc tggctgagcc gcatcgaacg
    21541 tgccgctgct gatggtcagt accgcatacg gcgtggcaat gcgttcttcg ccaatcgcca
    21601 gaccaccgta aaccatatgt ttcactgttg ctttaccgtc ctgtacgctg acggtgctgg
    21661 catcgttaga caccgccgct ttaaggcgat aacccagttt tgccgccagt aatttgccgc
    21721 gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg ctggcgaata gtgtcagcca
    21781 tgacaccggc gtaatcttcg atcatccgat cgtccggttt gccgtttaat ttccagacat
    21841 gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc attgaggaca aaggtgttga
    21901 tttgattagc taaagcctgc gcaccgttca tcagttccgg cagacgagaa ggggtatcgc
    21961 tgaataccca gacttgagaa aacgtgttca tagcatcccc tgtaattaaa tgactttgcg
    22021 aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg ccttcaatca cgatgcgctg
    22081 acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac caggctgcct ctgcgttaaa
    22141 accaatatcc gccgccgacc atacctggac gggctttttc gccgcgccga gaatggcttt
    22201 catcgaagga atttgtgggg aattgatatc agtggaaaca gcaacaaccg caggcagcgg
    22261 aatgcttaag gtttcggttt catcttccag ttcgcgctca acggtgaggg tatctgccgt
    22321 cagggagata attttgctga cgccgttaac tgccggaata ttgaggattt cgcccaccag
    22381 cagaccaacc tgctgggcat aaaggtcgga agaaccatcg ccacagagga tcaaatcaaa
    22441 gcctgctttc tgggcggctg cagccagtgc gctcgccgtt tgttgcggca gtgcctgctc
    22501 gaactggtca tcaatcacca caatcagttc atccgggccg cgcgatagta catctttacg
    22561 ccctttggcg ttggtcaggg ctttaccgcc cacacttaag gccgtcacct gcgcctctgc
    22621 tgcctgttgc tttagctggc aagccgcttc aatagcgttc agatcgtatt ggcttatttt
    22681 ggcatcggct ttgctgaagt ctaatgaacc atcagcatta ttgaccgcaa tatcctgttc
    22741 atcaggcacg cacttatagc aagtaataat cttcattgca tctccagaaa tcatgaagga
    22801 aacgtcgttc atatcggtgg tgttaattac agatatcacg ccctttaatt tggaaataaa
    22861 actttaatca ccaatattga aaatgtcaca cgcagataaa tccaactttc aatattgtta
    22921 agttcctcac caatattgaa aacatggcgt agaaaaaaga aattttcaat attgctttat
    22981 ggatcaccaa tattgaaagt cagatgctat ttttttactc acaacacaac ataacaaact
    23041 gattaataat caaaattaac gagaaaagcg ccctctattc acccgaaaaa aaatgtgata
    23101 acaatcacag aatgcagctt attgaatacc cattatgagt tagccattaa cgcgtccacg
    23161 aggttaataa taattatatc aaatgttaac aaaaataaaa caaacgggaa cgcaaaataa
    23221 atattcgttt tcacagtgga attaactcat gaagaatgaa aagagaaaaa cgggaataga
    23281 accgaaggtt ttctttccgc cgttaataat cgtcggcata ctttgttggc ttacagtcag
    23341 agatctggat gcagcgaatg tcgttattaa tgctgtattc agttacgtca ccaatgtatg
    23401 gggatgggca tttgaatggt atatggtggt gatgcttttc ggttggttct ggctggtgtt
    23461 tggcccgtat gccaaaaagc gtttaggtaa cgaaccacct gaatttagta ccgccagttg
    23521 gatctttatg atgttcgcct cctgtacgtc tgctgccgta ctgttctggg gatcgattga
    23581 gatctactac tacatctcca ccccgccgtt tggcttagaa ccgaactcga caggggcgaa
    23641 agagttgggg ctggcttaca gcttgttcca ctggggacct ctgccgtggg ccacttacag
    23701 cttcctttca gtcgccttcg cttacttctt ctttgtccgc aaaatggaag tgattcgccc
    23761 cagctcgaca ctggtgccgc tggtaggtga aaaacacgcc aaagggttgt tcggcactat
    23821 cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc
    23881 cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc attccgcata ccctgcaact
    23941 ggacgctatc atcattacct gctggattat cctcaacgcc atttgcgtcg cttgcggtct
    24001 gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg
    24061 ttgggtgttc attgtcagcg gtgccagctt catcatgaac tacttcaccg attcggtggg
    24121 gatgttgctg atgtatctgc cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg
    24181 cttcccgcag ggctggaccg tgttctactg ggcatggtgg gtgatttatg ctatccagat
    24241 gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat
    24301 ggtgctgggg ctgacagcgt caacctggat cctgtggact gtactcggta gtaacactct
    24361 gctgttgata gataaaaaca tcatcaacat tccaaatctg atcgaacagt acggtgtggc
    24421 gcgcgccatc attgaaacct gggccgctct gccactcagc accgccacca tgtggggctt
    24481 cttcatcctc tgctttattg ccaccgttac gctggttaac gcctgctctt ataccctggc
    24541 gatgtccact tgccgcgaag tacgcgatgg tgaagaacca cctctgctgg tgcgtatcgg
    24601 ttggtcaatt ctggttggca ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc
    24661 gattcaaacc gccattatcg ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac
    24721 gctctccttt attaaagacg cgaaacagaa ctggaaagat taattaaccc ccaaaatatc
    24781 aagaggttga aagatggatt ttaatttaaa tgatgagcag gaactgtttg tcgccggtat
    24841 ccgcgaactg atggccagcg aaaactggga ggcctatttt gccgagtgcg accgtgacag
    24901 cgtctacccg gaacgttttg tcaaagcact ggcggatatg ggtatcgaca gtctgctgat
    24961 ccctgaagag cacggtggtc tggacgcggg gtttgttact ctcgccgccg tgtggatgga
    25021 gctgggacgt ctgggggcac caacctatgt gctgtaccag ttgccgggcg ggttcaacac
    25081 cttcctgcgc gaaggcacac aagagcagat cgacaaaatt atggctttcc gcggcaccgg
    25141 taagcagatg tggaactcag cgattaccga accgggcgcg ggctccgacg tgggtagcct
    25201 gaaaacgact tatacccgta gaaatggtaa gatttatctt aatggtagta agtgttttat
    25261 taccagcagc gcctacaccc cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga
    25321 caaacctgtc tacaccgaat ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa
    25381 acttgaaaag ctcggtctgc gtatggatag ctgctgtgaa atcacctttg acgacgtgga
    25441 actggacgag aaagacatgt tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga
    25501 gttcgaccat gaacgtttcc tggtagccct caccaactac ggtacggcga tgtgcgcttt
    25561 tgaagatgcg gcgcgctacg ccaatcagcg cgtgcagttt ggcgaggcta ttggtcgttt
    25621 ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat
    25681 gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc acctctggcg atgcagcgat
    25741 gtgcaaatac ttctgcgcca atgcggcatt tgaagttgtg gatagcgcaa tgcaggtgct
    25801 gggcggtgtc gggattgcgg gcaaccaccg catcagccgc ttctggcgtg atctgcgtgt
    25861 agaccgcgtc tccgggggat ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct
    25921 gaagcaatac cgctaagtcc gtgcgctgcc tgatgcgacg ctaatgcgtc ttatcaggcc
    25981 tacgggagca cgaatgtagg ccggataaag cgtttacgcc gcatccggca gtcatgcgct
    26041 acataaattt ttcaggagct aataatggat catctaccca tgccgaaatt cgggccgttg
    26101 gccggattgc gcgttgtctt ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg
    26161 ttcgcagaat ggggtgcgga agttatctgg atcgagaacg tcgcctaggc cgacaccatt
    26221 cgcgttcaac cgaactaccc gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat
    26281 attttcaaag atgaaggccg cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc
    26341 atcgaagcca gcaaaggtcc ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg
    26401 cagcacaacc cgaaactggt tatcgctcac ctgtccggtt ttggtcagta cggcaccgag
    26461 gagtacacca atcttccggc ctataacacc atcgcccagg ccttcagtgg ttacctgatt
    26521 cagaacggtg atgttgacca gccaatgcct gccttcccgt ataccgccga ttacttttct
    26581 ggcctgaccg ccaccacggc ggcgctggca gcactgcata aagtgcgtga aaccggtaaa
    26641 ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc gtatgggcca gtacttcatg
    26701 atggattact tcaacggcgg cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac
    26761 tacgccggtt gcggtctgta taaatgcgcc gacggctaca tcgtgatgga actggtgggc
    26821 attacccaaa ttgaagagtg ctttaaagat attggcctcg cacatctact cggtacgccg
    26881 gaaatcccgg aaggcactca gcttatccac cgtatcgaat gcccttacgg cccactggta
    26941 gaagaaaaac tcgatgcctg gctggcggca cataccatcg cagaagttaa agaacgcttt
    27001 gccgaactga atatcgcctg tgccaaagta ctgaccgtac cggaactgga aagcaatcca
    27061 cagtatgttg cccgtgaatc aatcactcag tggcaaacga tggatggtcg cacctgcaaa
    27121 gggccgaaca tcatgccgaa attcaaaaat aaccccggac aaatctggcg cggaatgccc
    27181 tcacatggca tggacacggc tgccattttg aaaaatatcg gctacagcga aaacgacatt
    27241 caggagttgg tcagcaaagg tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg
    27301 cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa atggatagag gtgcaatgga
    27361 tatcattggc ggacaacatc tacgtcaaat gtgggacgat ctggcggacg tttacggtca
    27421 taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt aaccggtata gttatcttga
    27481 gttaaatcag gagattaacc gcacgacaaa cctgttttat acgctgggga ttcgcaaagg
    27541 cgacaaggtt gctctacatc tcgacaactg cccggaattt atcttttgct ggttcgggct
    27601 ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc ctgttgcgcg aggaaagcgc
    27661 gtggatcctg caaaatagcc aggcgtgcct gctggtgacc agtgcgcaat tctatcctat
    27721 gtatcaacag attcagcagg aagatgccac tcaattgcgg cacatttgcc tgacagatgt
    27781 ggcacttccc gctgatgatg gcgtgagttc gtttactcaa ctgaaaaatc aacaacctgc
    27841 caccttgtgc tatgcaccgc cgctatcgac tgacgatacg gcggaaattc tcttcacctc
    27901 cggcaccacc tcccgaccga aaggtgtggt gattacccat tacaacctgc gcttcgctgg
    27961 atattactcc gcctggcagt gtgcactgcg tgacgatgac gtctacctga cggtaatgcc
    28021 tgcgtttcat atcgattgcc agtgtactgc ggcgatggcg gcgttttctg ccggggccac
    28081 ctttgtgctg gtcgagaaat acagcgcccg cgccttctgg ggacaggtac agaagtaccg
    28141 cgccaccgtt accgaatgta ttccgatgat gatccgtacc ttgatggcgc agccgccttc
    28201 agcgaacgat cggcaacacc gcctgcggga agtgatgttt tatctcaact tgtcggagca
    28261 ggaaaaagat gcgttttgtg aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac
    28321 ggaaaccatt gtgggcatta tcggcgatcg ccctggcgat aaacgacgct ggccgtcgat
    28381 tggtcgggcg gggttttgct acgaagcgga gatccgcgac gatcacaatc gcccgctccc
    28441 ggcaggtgag atcggtgaaa tctgtattaa aggcgtacca gggaaaacca tcttcaaaga
    28501 gtattttctc aacccgaaag ccactgcgaa agtgctggaa gccgacggct ggctacatac
    28561 cggcgatacc ggatactgcg acgaagaggg ctttttttat ttcgtcgatc gccgctgcaa
    28621 tatgatcaaa cgcggtggcg agaatgtctc ctgcgtggag ctggaaaata tcatcgccac
    28681 ccatccaaaa attcaggata tcgtggttgt gggtattaaa gattcgattc gcgatgaagc
    28741 catcaaagca tttgtggtgc tgaatgaagg tgaaacattg agcgaagagg aatttttccg
    28801 cttctgcgaa caaaatatgg cgaaatttaa agtgccctct tatctggaga tcagaaaaga
    28861 tctgccacgt aattgctcgg ggaaaataat tagaaagaat ctgaaataaa tgaaacagca
    28921 gggaactacc ctgccaacta ataatcacgc tataaaacaa tatgcatttt ttgcagggat
    28981 gttgtcgtcc ctgaaaaagc aaaaatggag aaaaggaatg agtgaatcat tacatctgac
    29041 ccgcaatgga tcaattctgg aaattaccct tgatcgtcca aaagcgaatg ctattgatgc
    29101 aaaaaccagc tttgaaatgg gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg
    29161 tgtcgccatt attaccggtg ccggagagaa gttcttttcc gcgggctggg atttaaaagc
    29221 ggcagcagaa ggcgaagcac cggatgctga ctttggcccg ggtggatttg cgggattaac
    29281 cgaaattttc aatctcgaca aaccggttat cgcagctgtg aacggctatg cctttggcgg
    29341 cggtttcgaa ctggcgctgg cggcagattt tattgtttgt gccgataacg ccagcttcgc
    29401 cctgccggaa gccaagctgg gcatcgttcc tgacagcggc ggcgtgctgc gtctaccgaa
    29461 gatcctgccg cctgccatcg tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga
    29521 agaggcgctg cgttggggga tagtcaaccg cgtggttagc caggcggaac tgatggataa
    29581 cgcccgcgaa ctggcgcagc agctggttaa cagcgccccg ctggcgattg cggcgctgaa
    29641 agagatcttc cgcaccacca gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag
    29701 cggcgtgttg aaacactatc cgtcggttct gcattcggag gatgccattg aagggccgct
    29761 ggcgtttgcc gagaagcgcg atccggtgtg gaaaggacgt taacaaccgt gagctattac
    29821 gcctttgagg ggttaattcc ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc
    29881 ttgattggcg atgtgattgt gggagccggt gtctacatcg gcccactcgc ctcactgcgt
    29941 ggtgactacg ggcggttgat cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg
    30001 catggctact gcgacactga caccatcgtt ggggaaaacg gccatatcgg gcacggagcg
    30061 atcctgcatg gttgtgtgat tggtcgcgat gcattggtcg ggatgaacag cgtgattatg
    30121 gatggcgcgg tcattggcga agagagcatt gttgccgcca tgagctttgt caaagcaggc
    30181 tttcacggcg agaaacgcca gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt
    30241 agtgacgacg agttacactg gaaacggttg aataccaaag agtatcagga tcttgtcggg
    30301 cgctgtcatg catcgttaca tgaaacacag ccactgaggc aaatggagga aaatcgcccc
    30361 cgtttgcagg ggacgacgga tgtgacgcct aaacggtgat cttgccggat gcggcgtaaa
    30421 cgccttatcc aactctgctg gagagcaacc gtaggccgga taagatgcgc cagcatcgca
    30481 tccggcgata gctgtcggat gcggcataaa cgccttatcc aacccggctg gagagcaacc
    30541 gtaggctgga taagatgcgc cagcattgca tccagcaacc aggctgcgtg gtatcaacga
    30601 aatgacatta acgacgcata ctctttgaca acatcatctg ccacttctgc tcgcggttaa
    30661 gttccgtgag cggaacggca ggaatacgcg gcgtctttct tacgccgact aatttatccc
    30721 ggctgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact tttaccagtc
    30781 gctggcactg gcatccccgt ccttccagct tattagggat catcttaact gcgcagctta
    30841 tttctgcgac ttccgacaga atataagtca gggtattaac cgccttgctg tgttcaatat
    30901 cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc atccattcgg
    30961 cgattaacaa gtagggtggt ttttcaacat atccttcaca catacgaact ccagtttcac
    31021 aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat attaaaattg
    31081 tggatatatc gatagtatgc aacttcaccg tcgataa
//
LOCUS       DAZOZY010000026        30870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZOZY010000026 DAZOZY010000000
VERSION     DAZOZY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.026"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            470..985
                     /locus_tag="ACHURQ_001061"
     CDS             470..985
                     /locus_tag="ACHURQ_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0822899.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1203..1931
                     /gene="artP"
                     /locus_tag="ACHURQ_001062"
     CDS             1203..1931
                     /gene="artP"
                     /locus_tag="ACHURQ_001062"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR0822900.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1949..2680
                     /gene="artI"
                     /locus_tag="ACHURQ_001063"
     CDS             1949..2680
                     /gene="artI"
                     /locus_tag="ACHURQ_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR0822901.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2687..3403
                     /gene="artQ"
                     /locus_tag="ACHURQ_001064"
     CDS             2687..3403
                     /gene="artQ"
                     /locus_tag="ACHURQ_001064"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR0822902.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3403..4071
                     /gene="artM"
                     /locus_tag="ACHURQ_001065"
     CDS             3403..4071
                     /gene="artM"
                     /locus_tag="ACHURQ_001065"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR0822903.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4363..5094
                     /gene="artJ"
                     /locus_tag="ACHURQ_001066"
     CDS             4363..5094
                     /gene="artJ"
                     /locus_tag="ACHURQ_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR0822904.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5269..6396)
                     /gene="rlmC"
                     /locus_tag="ACHURQ_001067"
     CDS             complement(5269..6396)
                     /gene="rlmC"
                     /locus_tag="ACHURQ_001067"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR0822905.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6437..6925)
                     /locus_tag="ACHURQ_001068"
     CDS             complement(6437..6925)
                     /locus_tag="ACHURQ_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR0822906.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6985..7830)
                     /gene="potI"
                     /locus_tag="ACHURQ_001069"
     CDS             complement(6985..7830)
                     /gene="potI"
                     /locus_tag="ACHURQ_001069"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR0822907.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7827..8780)
                     /gene="potH"
                     /locus_tag="ACHURQ_001070"
     CDS             complement(7827..8780)
                     /gene="potH"
                     /locus_tag="ACHURQ_001070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR0822908.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8790..9923)
                     /gene="potG"
                     /locus_tag="ACHURQ_001071"
     CDS             complement(8790..9923)
                     /gene="potG"
                     /locus_tag="ACHURQ_001071"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR0822909.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10018..11130)
                     /gene="potF"
                     /locus_tag="ACHURQ_001072"
     CDS             complement(10018..11130)
                     /gene="potF"
                     /locus_tag="ACHURQ_001072"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR0822910.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11481..11957)
                     /locus_tag="ACHURQ_001073"
     CDS             complement(11481..11957)
                     /locus_tag="ACHURQ_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR0822911.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12045..12947)
                     /gene="rimK"
                     /locus_tag="ACHURQ_001074"
     CDS             complement(12045..12947)
                     /gene="rimK"
                     /locus_tag="ACHURQ_001074"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR0822912.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13008..13730)
                     /gene="nfsA"
                     /locus_tag="ACHURQ_001075"
     CDS             complement(13008..13730)
                     /gene="nfsA"
                     /locus_tag="ACHURQ_001075"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR0822913.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13714..14001)
                     /locus_tag="ACHURQ_001076"
     CDS             complement(13714..14001)
                     /locus_tag="ACHURQ_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR0822914.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14161..14418
                     /gene="grxA"
                     /locus_tag="ACHURQ_001077"
     CDS             14161..14418
                     /gene="grxA"
                     /locus_tag="ACHURQ_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR0822915.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14448..14825)
                     /locus_tag="ACHURQ_001078"
     CDS             complement(14448..14825)
                     /locus_tag="ACHURQ_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR0822916.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            15095..16780
                     /locus_tag="ACHURQ_001079"
     CDS             15095..16780
                     /locus_tag="ACHURQ_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR0822917.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(16955..17491)
                     /gene="rcdA"
                     /locus_tag="ACHURQ_001080"
     CDS             complement(16955..17491)
                     /gene="rcdA"
                     /locus_tag="ACHURQ_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR0822918.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17575..18783
                     /locus_tag="ACHURQ_001081"
     CDS             17575..18783
                     /locus_tag="ACHURQ_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0822919.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            18783..19598
                     /gene="ybjI"
                     /locus_tag="ACHURQ_001082"
     CDS             18783..19598
                     /gene="ybjI"
                     /locus_tag="ACHURQ_001082"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR0822920.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19684..19968
                     /gene="ybjH"
                     /locus_tag="ACHURQ_001083"
     CDS             19684..19968
                     /gene="ybjH"
                     /locus_tag="ACHURQ_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR0822921.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(20009..21236)
                     /gene="mdfA"
                     /locus_tag="ACHURQ_001084"
                     /pseudo
     CDS             complement(20009..21236)
                     /gene="mdfA"
                     /locus_tag="ACHURQ_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            21521..22117
                     /gene="ybjG"
                     /locus_tag="ACHURQ_001085"
     CDS             21521..22117
                     /gene="ybjG"
                     /locus_tag="ACHURQ_001085"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0822922.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            22175..22933
                     /gene="deoR"
                     /locus_tag="ACHURQ_001086"
     CDS             22175..22933
                     /gene="deoR"
                     /locus_tag="ACHURQ_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR0822923.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(22980..24182)
                     /gene="dacC"
                     /locus_tag="ACHURQ_001087"
     CDS             complement(22980..24182)
                     /gene="dacC"
                     /locus_tag="ACHURQ_001087"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR0822924.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24429..25054
                     /gene="gstB"
                     /locus_tag="ACHURQ_001088"
                     /pseudo
     CDS             24429..25054
                     /gene="gstB"
                     /locus_tag="ACHURQ_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(25051..26166)
                     /gene="yliI"
                     /locus_tag="ACHURQ_001089"
     CDS             complement(25051..26166)
                     /gene="yliI"
                     /locus_tag="ACHURQ_001089"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR0822925.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26277..26660)
                     /gene="bssR"
                     /locus_tag="ACHURQ_001090"
     CDS             complement(26277..26660)
                     /gene="bssR"
                     /locus_tag="ACHURQ_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR0822926.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26873..28198
                     /gene="rimO"
                     /locus_tag="ACHURQ_001091"
     CDS             26873..28198
                     /gene="rimO"
                     /locus_tag="ACHURQ_001091"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR0822927.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28245..29566)
                     /locus_tag="ACHURQ_001092"
                     /pseudo
     CDS             complement(28245..29566)
                     /locus_tag="ACHURQ_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(29574..>30870)
                     /locus_tag="ACHURQ_001093"
     CDS             complement(29574..>30870)
                     /locus_tag="ACHURQ_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR0822928.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7780 a         7963 c         7480 g         7647 t
ORIGIN      
        1 tcgagagtca aagctgggac tatcaaattt cgctcatttt caaaaataca acgtaatttt
       61 ggcaatcatc ttaatcgcag gatctcttat cgcatgtgaa aaattttgta atgattagac
      121 aataaccgcg ttcatttatc acgcgttata aatgtgaaac tggcttcttg cagatgctct
      181 taacctctac aggaggattt ataccaccat ttcagaacta tcatatttac cttaaatgat
      241 catttcagcc atataacacg ctcaacctca caaatggttt acactgtcta ttgtttgggt
      301 aatttaagta agtcagctac gagcaacctt gagtggatag ccagcaatgg actcaaaccc
      361 aatcaggtgt tcagattgtt tcagaattgt attaaaaaca atgtattaaa taatctgata
      421 gcgtttggtc gtagtgtgac gcagacagca agctaataag gaaatgatta tgcgctaccg
      481 ttcactatct ctacttattc catgtgcact gctgctgagt gcctgtacca ccgtcacgcc
      541 agcttataaa gataacggtc cccgcaccgg ttcctgtgtc cagggggggc ctgacagcgt
      601 ggctcaacaa ttctatgact accgcattca acatcgcagc aatgacatta ccgccctgcg
      661 cccctatttg agtgacaaac tggcaaccct tcttagcgat gccagccgcg ataatagtca
      721 tcgtgaattg ttaagcagcg atccgttctc cagtcgaacc accttgccag atagcgccca
      781 cgttgccagt gcatcgacta tccctaatcg tgatgcccgt aacattccat tgcgcgtaga
      841 tctcaaacag ggtgaccagg gttggcagga tgaagtgtta atgattcagg aaggccagtg
      901 ttgggtcatc gacgatgtgc gctacttagg cggcagcgtt catgccacgg cgggtacgct
      961 ccgtcagtcc atcgagaatc gctaacgcct tgatcaacca ataaaccccg taaattgtcc
     1021 ggcatttcgt gaggaatgcc gacatttatg ctcgccgacc accgcccccg ttattttgtg
     1081 ctatgtttat tgaataatgc gctttgcttt taacttttaa agcagaaata ttgcataatt
     1141 attctgtcaa aggtactatc ttcgacctca atagctatca gactgccagt atacgagtgt
     1201 caatgagtat tcaattaaac ggcattaatt gcttctacgg cgcgcatcag gcgctgttcg
     1261 atatcacgct ggattgccca cagggcgaaa cgctggtgtt acttggcccc agcggcgcgg
     1321 gtaaaagctc gctgctgcgt gtactcaatc tgcttgagat gccacgctcc ggtacgctca
     1381 acattgcagg caaccatttc gatttcacca aaacaccctc tgacaaagcg attcgcgatt
     1441 tgcgtcgtaa cgttggcatg gtgtttcagc aatacaacct gtggccgcat ctgaccgtgc
     1501 agcaaaacct gattgaagcg ccctgccgtg tactggggtt gagtaaagat caggcgctgg
     1561 cccgtgcaga aaaactgctg gaacgtctgc gtctcaaacc ttatagcgat cgttacccgc
     1621 tgcatctttc tggtggtcag cagcagcgtg ttgctattgc ccgtgcgttg atgatggaac
     1681 cgcaggtact gctgttcgat gaaccgaccg ccgcactgga cccggaaatt acggcacaaa
     1741 tcgtcagcat cattcgtgag ctggcagaaa cgaatattac ccaggtgatc gtcacccacg
     1801 aagttgaagt ggcgcgtaaa accgccagcc gagtggtgta tatggaaaat ggtcatatcg
     1861 tagaacaagg cgacgcgagc tgctttaccg agccgcaaac cgaagcattt aaaaactatc
     1921 tctctcacta agattcggga taacaacaat gaaaaaagtt ctgattgccg cgttaattgc
     1981 aggttttagt ctttccgcca cagctgccga aaccattcgt tttgctaccg aagcatccta
     2041 tcctccgttt gaatcgattg atgcaaacaa ccagatcgtt ggttttgacg tcgacctggc
     2101 acaagcgctg tgtaaagaga ttgatgcaac ctgcactttc tctaaccagg cgtttgacag
     2161 cctgatccca agcctgaaat tccgtcgcgt agaagccgtg atggcgggca tggatatcac
     2221 tccggagcgt gaaaagcagg tgctgtttac caccccgtac tatgacaact ctgccctgtt
     2281 tgtgggtcag caaggcaaat acaccagtgt tgatcagctg aaaggcaaaa aagtcggcgt
     2341 acagaacggg acgacacacc agaaattcat tatggataag cacccggaaa tcactaccgt
     2401 tccgtatgac agctaccaga acgcaaaact ggatctgcaa aacgggcgta tcgacggcgt
     2461 cttcggtgac accgcagtgg tcactgagtg gctgaaagat aacccgaaac tggcggcggt
     2521 gggcgacaaa gtgaccgata aagattactt cggcactggc ctcggcatcg cggtacgtca
     2581 gggcaacact gagctgcagc agaaactcaa cactgcgctg gaaaaagtga agaaagatgg
     2641 cacttacgaa accatctaca acaaatggtt ccagaagtaa ttcctgatga atgaattttt
     2701 tcctttagca agcgccgccg ggatgaccgt cggccttgcc gtttgtgcat tgattgtcgg
     2761 gctggcgctg gcgatgttct ttgcggtatg ggagtcggca aaatggcgtc ctgtcgcgtg
     2821 ggcaggttca gcgctggtaa ccattctgcg tggcctgcca gaaattctgg tggtgctgtt
     2881 tatctatttt ggctcctcgc agctgctgct gacgctttcg gatggcttca ctatcaatct
     2941 tgggttcgtg cagatcccag tgcagatgga cattgagaac ttcgacgtta gcccgttcct
     3001 ttgtggtgtc atcgctctgt cactgctgta tgccgcctat gcctcgcaaa cgcttcgggg
     3061 cgcgttgaaa gcggtgccgg tgggtcagtg ggagtctggt caggcgctgg ggctgtcgaa
     3121 atcggctatc tttttccgtc tggtgatgcc gcagatgtgg cgtcatgcgc tgcctggcct
     3181 cggtaaccag tggctggtgc tgctgaaaga taccgcgctg gtcagtttga ttagtgtgaa
     3241 tgatttaatg ctgcaaacaa aaagcatcgc tactcgtacc caggaaccat ttacctggta
     3301 cattgtggcg gcggcgattt acctggtgat caccctgctc agtcagtaca ttctcaaacg
     3361 cattgacctg cgcgcgacac gttttgagcg gaggcccagc taatgtttga gtatttaccc
     3421 gaactgatga aagggctaca caccagcctg acgctaaccg ttgcctcgct gattgtggca
     3481 ctgattctgg cattgatttt taccatcatc ctgacgctga aaacgccggt gctggtgtgg
     3541 ctggtgcggg gttatatcac gctgtttacc ggtacgccgc tgctggtgca gatcttcctg
     3601 atttattacg ggccgggcca gtttccgacg ttgcaggagt atccggcact gtggcatttg
     3661 ttgtcagaac cgtggttatg tgcgctgatt gcgttatcgc tgaacagtgc ggcgtatacc
     3721 acgcagctgt tttacggtgc aattcgtgcg atcccagaag gtcagtggca gtcctgtagc
     3781 gccctgggaa tgagcaaaaa agatacgctg gcgctcctgc tgccgtatgc ctttaaacgc
     3841 tcgctctctt cttattccaa cgaagtggtg ctggtgttca aaagtacctc tctggcttac
     3901 accattacgc tgatggaagt gatgggatac agccagttgt tgtacggacg cacctacgat
     3961 gtaatggtgt tcggtgcggc agggattatt tacctggtcg tcaacggcct gctgacgctg
     4021 atgatgcgtc tgatcgagcg caaagcactg gcattcgaac ggcggaatta atgtagctgc
     4081 gtacattcgc cgggggggtt tgtaggtctg ataagacgcg ccagcgtcgc atcagacatc
     4141 agcacggtgc ctgtgccgga tgcggcgtgg acgccttatc cggcctacat agtgcataaa
     4201 ttcttataat gaagacggac aactcactaa gttatccgtc tttttttatt tcatttaaat
     4261 tatttaatca tatttattgc atataaattc acttgatggc attgttatcc catgccgcag
     4321 acacggccaa aaatcataag attgacagac gggagttcca tcatgaaaaa gttagttctt
     4381 gccgctttac ttgcttcctt tactttcggt gcttctgccg cagagaaaat caattttggc
     4441 gtttcagcca cctatccacc ctttgaatct ataggtgcta ataatgagat tgtcggcttt
     4501 gatatcgatc tggcaaaagc cttgtgcaaa caaatgcagg cagaatgtac ttttactaat
     4561 cacgcgttcg acagcctgat cccgtcactg aaattcagaa aatatgactc cgtaatctcc
     4621 ggtatggata tcacgcccga gcgtagcaag caggtatcgt ttaccacgcc ctactatgaa
     4681 aactcagccg tcgtgattgc caaaaaagat acctacaaaa cgtttgccga tctgaaaggc
     4741 aaacgtattg ggatggaaaa cggcaccacg caccagaaat atattcagga tcagcacccc
     4801 gaagtgaaaa ctgtctccta tgacagttat cagaatgcct ttatcgatct gaaaaatggt
     4861 cgtattgatg gggtatttgg tgacacagcg gtggtaaacg aatggctgaa aaccaatccg
     4921 caactgggcg ttgctactga aaaagtgacc gatccgcaat acttcggcac cggtctgggc
     4981 atcgctgtac gtccggataa caaagctctg ctggaaaaac tgaataacgc gctggcagca
     5041 attaaagctg acggtacata tcagaaaatc aatgaccagt ggttcccgca gtaattgaga
     5101 ctgatgacaa aagtaagatt gcctgatgcg ctgcgcttat ctggcccaca tcaactctgc
     5161 aatgtattga tttagcatga ttttgtaggc cggataaggc gtacacgccg catccggcat
     5221 gaacaaagcg cacctgatca gcaatctgaa gccgcacctg cggctttttt attgcttcac
     5281 cagcagcgtc atcacttcat agtgcgcggt gtgcgggaac atatcaaaaa gctgtacccg
     5341 ttcgatgcgg taacctggca gttcgcggat atctttcgcc atggtttggg cgttacagct
     5401 ggagtagata ataaaacgcg gtgccatcgt tgagagataa tcacacagcg gtttaccaat
     5461 gccgcggcgc ggcgggttaa ccagcaccag ctccggcaca tccccctgag cggtggcaaa
     5521 ctgagtggag tccagcgcct gaaattgcaa acgcgttaag cccagttcag cggctgactg
     5581 ctttgcacag gcaatggcct ctgatgcaat ttcgatcccg gttaactgca tgtcaggcgt
     5641 cgcgcagtgt aaaccaaagc cacccacacc gcagaacaga tcccacatat gtttaactgg
     5701 cagctgccgt acccagtcgc gcgcggtagc gtacagctgg ctggcgaccg ccggattagt
     5761 ctggaagaaa ctttgcggac ggatccacag cggtacgtca ttaaaacgct ctgccagcgc
     5821 ctgttgttca gtcaggtaga tctccgtctc cccttccata atcgccatat gtaccggctg
     5881 aatattgacg gtaataactt tcagttgcgg tagttgttcc tgtaaccacg gcagcgcctt
     5941 acgcagttgt gccagtttgg tttcagaacg cagtacaaag cgcagcatca tgccgccatc
     6001 gctctggctt tcagtcagca gaatgtattt cagttcgcca cgcttacgcg ccacgttgta
     6061 gggcgttaac cccgcacggg cgataaacgg ttttagcgcc gcaaaaacgg gcgcaaaaga
     6121 ggcaggataa agcgggcagt cacaaaggtc ttccggcgtg ccatcgcgat gcagcatacc
     6181 gagcagtggt ttttcaacgc taccactcac caccattttg gctttattac gaaacccttg
     6241 ttccgggcct gacaccggcg cgcaccattc ctcaaccgga aaatcggcga gcagattttt
     6301 aagatcggcg gttttagcgg agagttgctc tggaatcggc tgcgttatcc actgacagga
     6361 acgacagcga cccgcgtcgt aaagtgcgca ctgcatacat tgaccttcac atcatcaggg
     6421 gcgacgatta tacacattat tgcaactgga agaaccgccg actggtcgag ggaacgaaca
     6481 gcagcatcag gatgagcata tccggcagct tctgcagcat caggctatgg aagatttcac
     6541 gttttgattc accgggaatg ctgaacagct ccggataacc gtaccccagc gaagccgccc
     6601 acaagtaact tgcggcggtg atttgtgtca gcagatatag ccagcgcgcc cagcgacgcc
     6661 ctttcaccag tgaaaacgca caccagatct caatgaaaac cagcaccaga ctgcttaaaa
     6721 agaccagcgt taaactccag gtttgtacgc tgcgatgaat gaactcgcca ataccgcgca
     6781 cgcccagcgt attgaaaatc atcagcacgt cgaggccacg gatcataata atggcgagcg
     6841 ccgccacctg caccagcgca ggcacattca ggcgagcatg agatgaagat gttttcttaa
     6901 aaaatcccaa cgtttcgtct tccatgaaaa caatgccgcg gcatgcgcgg cattatgtag
     6961 ccaggttggc aaattttagt gtcttcagcc atgtcttgca cgctggatat cgcgtatccg
     7021 ctgtttttct gcgcgagcca tcagatacca ggcgataaat ccgacaattc cgaccgcacc
     7081 gagaattaac gttgcaaggg cgttgatttc cggattcact cccatccgca cgctggaaaa
     7141 gaccagcatc ggtaacgtgg tggctcccgg cccggaaaca aagctggcga tcaccagatc
     7201 atcaagcgac aaagtaaaag ccagtaacca gccagaaatg atcgcgggca tgatcatcgg
     7261 tagcgtaatg acgaaaaaca ctttcagcgg cgtcgcaccg agatccatcg ctgcttcttc
     7321 tatcgagcga tccagttccc gcaatcttga agaaataacg accgccacat aagccgtaca
     7381 aaaagtgaca tgcgccagcc agatggtgag cataccgcgg tccgcaggcc agccaatggc
     7441 atgagcaagc gcgacgaata ataacaacag cgacaagccc gtgatgacat ctggcatcac
     7501 cagcggcgcg gtgatcataa aggcaaaacc atttgatccg cgaaacctgc caaaacgcac
     7561 cagcaccacc gccgcaatag tcccgaggat cgccgccgcc gttgccgcac aggcagcaat
     7621 tgtcaggctt aaaccaaccg cactcatcat cgcgtcatcg cgcaataact caccatacca
     7681 gcgcgttgac cagccggccc acaccgtcac cagcttcgag ctgttaaacg aatagataac
     7741 cagcatcagc attggcgcgt agagaaaggt aaagcccagc agcaaaatca caatccgcca
     7801 gggcgaacga actaccggta aattattcat ccgtgttctc ccacgctttt ttgctggtgt
     7861 ttgtggaacc acattatcgg cacaattagc agcagcaaca tgatgatcgc taccgccgag
     7921 gccaccggcc agtcgcggtt attgaaaaac tcttgccata acacgcgccc gatcatgatg
     7981 ctgtccgggc caccgagcag ttccgggatc acaaactcgc ccacagccgg gataaacacc
     8041 agcatcgatc cggcaataat cccaccttta gtcagcggca cgatcacagt aaagaacgtt
     8101 ttcagcggtc gtgcaccgag atccagcgct gcttccacca gcgaataatc aatacgaatc
     8161 aacgcggtat aaatcggcag taccataaac ggcacgtaag cgtaaacaat gccgatataa
     8221 acggccagat tggtatgcag aatggtcagc ggttgatcga taacccccag ccacagcaga
     8281 aaattattca gcacaccgtt gttttttaat attcccatcc aggcataaac gcggatcaga
     8341 aacgaggtcc acgacggcag gatcaccagt agtaataaaa tattacgggt cgaaggctta
     8401 ctgtgcgcca ccgcccacgc cagcggatag ccgatcagta aacagcaaaa tgtcgaaatc
     8461 gccgccacct gtaacgactg gagataagca tcaaaataga gcggatcgtc ggtcagttgc
     8521 agaaaattac cgagattaag agtgatggaa agttgcccgt cagcccactc catcagttcg
     8581 gtataaggtg gaatagcgcg cgccatctct cccaggctta ttttaaagac aatcagaaat
     8641 ggcagcagaa acagcaagat caaccagata tatagcaacg caatgaccag tttgcgccca
     8701 tgcttcattt gcagctgcga caaccacagc ttaaatccgc ccggcggttt cgactgggca
     8761 gcaggttcaa gtgtactcat ctttgctcct taaaccgtca gcaccacaca gctgtccact
     8821 tcccagcaca aacgcacttc gtcgccccag gtcggtaacc ctttacgatg acgatgggcg
     8881 ttttgtagct gggcgctgat catctgccca cttttcagtc gaacgtgata caccgaaaga
     8941 tcgccgagat aggcaatgtg tatcacctcc cccaccgcga agttacaacc attggcgggc
     9001 ggctcttcgc aaagcatgat tttttccggg cgcagcgcta cgtgtaccgg cacgttatcg
     9061 accactgaag catccgcatc gactttcagt ggatgcacca gccccggcga atcaagcacc
     9121 aggccatctt cctgacgctc tttgagtaca ccttcaaaga cattaaccga accgataaat
     9181 tcagcgctgt agcgggtagt tggatgctcg tagatctctt ccggctcgcc aatctggaca
     9241 aatttcccgc gattcataat ggcgatacgc cccgccatgg tcatcgcctc ttcctgatcg
     9301 tgggtgacca tcacacaagt cacaccgacg cgctccagaa tatccaccac ttcaagctgc
     9361 atccggtcac gcagcttttt atccagcgcg cccatcggct catcgagcag taatagtttc
     9421 gggcgtttgg caaggcttcg ggccagggcc acacgctgtc gctgaccacc ggaaagctga
     9481 tgcggtttgc gtttggcgaa ctcctgcatg tgcaccaggc cgagcatctc atggacccga
     9541 ctggcaattt ccgctttcgg tagtttgtcc tgtttcaggc caaaagcgat gttctgttcc
     9601 acggtcatat gcggaaacag cgcgtaagac tgaaacatca tattgatggg gcgcaggtaa
     9661 ggcggcacct gtgacaaatc gacgccatcg agcattatct gcccggcaga aggttgttca
     9721 aaacctgcca gcatacgcag cagcgtggac ttgccacagc cggatgcgcc cagcagcgcg
     9781 aagatttcac ctttgtagat ggtcaggctg acatcatcga ccgcatgctg accatcgtag
     9841 gatttggtga ggttgcggat ttctaatagc ggcgtcagcg ccttacgggt tttcgcctgc
     9901 gggcgaggga tagcgtcatt cacggctgct ctccggcatt gaatgtgtgc aagcgcacca
     9961 tgatggtgcg aattgccggc tggtgtggtg cgcccgcccg gcatctaagg ctgcggatta
    10021 ttttccgctc ttcactttgg tccacgcgcg ggtgcgcaca cggtcgattt tcggatcctg
    10081 tactttcaga gtgaatagct tcgcacgaac gtccgccgga ggataaatac ccgggttctc
    10141 acggacttcc gcactcacca gcggcgtggc tgctttattg gcgttggcat agaacacatg
    10201 gtcggaaata tgcgctacta catccgggcg cagcaggtaa ttcaggaact gataggcttc
    10261 gtctttgttt ttggcatccg caggcatggc gaatacatca aagaacgcca tcgccccttc
    10321 ttttggaatc gagaacgaga cattcactcc attcttcgct tccttcgcgc ggtttgacgc
    10381 ctgccagaca tcacctgccc agccgatagc gacgcaaata tcgccgtttg ccaggtcgtt
    10441 aatgtattga gatgaatgga aataacgaat gttcgggcgc agctttaaca gcagatcggt
    10501 tgccggtccg gtgtaatcat ccgctttagt gctgttggga tctttgccga gataattcaa
    10561 cacggtagca aaaacttctt ctggcgcatc caggaaagag acaccgcagc ttttcagttt
    10621 ttccagattt tcaggtttga ggatcaagtc ccagctatcg acgggcgcgt tttcgcccag
    10681 cacagcttta actttatcaa cgttatagcc aatcccggtc gtcgcccaca tatagggcat
    10741 agcaaattta ttgtcgggat cgtgtttggc gaccagcttc agcagctccg ggtcgagatt
    10801 cttccactcc ggcaatttgc ttttgtccag cggctggaaa actcccgcag tcaactggcg
    10861 ttcaagaaag ctggcagatg gaaccaccaa gtcaaagccg gtgctacccg ccattaattt
    10921 gccttccagt acttcgttag agtcgaaaac atcgtagacg actttaatac cggtttcttt
    10981 ttcaaaattg gccaccgtgt ccggggcgat ataatcagac cagttataaa tgtggagtgt
    11041 tttttgttca gccgcgagcg tgccgacaga gacggccatc agagcacccg cagccagacc
    11101 cgaaagccat tttttattta aggcggtcat atctctttcc ttctgaaagt tcgttaacaa
    11161 acgaacaaaa gttgtcacac taaaagatat gcaataaacg tgcatatttt ttatcggcgg
    11221 ggtgtaagaa aagagaactc tctcccggcc agcactgctc gctatataaa agcatcgcaa
    11281 gaataacttt agccagggtt tgagactata gctcggatta aaaaacggta tttaacaaat
    11341 ttttatgcgt atttgctcta tgtgataaag ccaaatcgac acaagagaag tgaaataatc
    11401 tttaagaaaa ggttaaaaac agcagataat tttgcagcgc gcagccactg caccagcgac
    11461 tgcgtgctcg aaaggagtta tcagtggagg aaatagtttt cggtaacgtt gttttgccct
    11521 tcatcgtcag tcggtaacag cagttgatgg gcattagctt caagaatgac catcgaaatc
    11581 tgctcttcgc tctgacgcac gaaccatgca aactgctcat aggttacgcc ctgcataacg
    11641 gataaagact ggcaaaccac cagctttggc agattatcat cctgcatatc aagaaatgct
    11701 ttcacggtca gcgaactggc attgatggca gataaatccg ccgccagcgg caatacggct
    11761 gaaggtcgga cttccgccat ggcagaaaac aggatcgtgt tatcgatcag atcgattttg
    11821 gcatcaaaga caccgtcgaa attctgcata tggggcagat gcagagcctg acagttatca
    11881 cattcaaaaa aactcatccc caggtcatca agccattgac gcagcgtatc cagaccagga
    11941 acgaccagcg atgtcataat tttgtacaac ctctttcgat aaaaagaccg gcacagctta
    12001 cgcaaaaagc gcaggcaaaa ccatgatcag taatgtgatt gcgactaacc acccgttttc
    12061 aggcaatatt ctgtcgtagc gtggcgttcg atccagcgga tcattttacc cgcgatgtcg
    12121 ataccagtgg ttttttctat tccttccagc cccggcgacg cattcacttc catcaccaac
    12181 ggcccgcgat tagcacgcag aatatccaca ccagcaacgt ccagcgccat cgttcgcgcg
    12241 gcttttatcg cgatttcacg ctcctgtggt gtgatactgg cgacgcttgc cgcgccgcca
    12301 cgatgcaaat tggaacgaaa atcgccctct ttcgcccgcc gttcaatcgc agcgaccact
    12361 tcatcgccaa caaccagaca gcggatatcg cacccttgcg cctctttgat atattcctgc
    12421 accagaatat gcgcgttcag accgcggaaa gcgtcaatca cgctttccgc cgcctgacgc
    12481 gtctccgcca gcacgacgcc aattccctgc gtgccttcaa ccaacttgac caccagcggc
    12541 gcaccaccga ccatgtcgat taaatcgctg gtatcatccg gcgaatgcgc aatgcccgtg
    12601 acaggcaggt cgatgccctg acgcgccagc agttgcatgg aacgcaattt gtcacgcgcc
    12661 cgggcaatgg cgaccgactc attgagcgga tagctcccca gcatctcgaa ctggcgcagt
    12721 gccgccgtcc cataaaaggt aatggcggtg ccaatacgcg ggatcactgc gtcaaaatgg
    12781 ggtaacttgc ggcctttgta gtgaatagaa gacgccgcag gatttatgtt catgtagcaa
    12841 gaaagcggat caagaatttc aaccaggtga ccgcgctgta tagcggcttc acgcagccgc
    12901 ttacacgaat agagcgttcc atcccgggac aatatggcaa ttttcaccct gcacctctct
    12961 gtcagacctg gtcaaaagcc cgcgtatcat acatcgacgt ggcggtttta gcgcgtcgcc
    13021 caaccctgtt tgtgcaaata atccagaata aatgggcggc tttctttaat gattgttcgg
    13081 cggatatgat cgctccaggt atcccggcga ttattgctgc cacgggtgag gtaatattcc
    13141 gccagttgct cgtcatactg cgccagtgcg tctttatcca gcggttgata gctgttttca
    13201 tgcaccaaaa tggaggccgg taaacgcggc ttgagatccg gattatccgc aggccagcca
    13261 aggcacagcc caaacagcgg cagaacatgc tgcggtaatt tcagcagttt cgtcaccgct
    13321 tcaatattat tgcgcaggcc gccgatatat accccgccca atcccagcga ttccgctgcg
    13381 gttaatgcat tctgcgccat cattgccgta tcaacgacac cgagcaacag ttgttccgcc
    13441 aggccgagct gagcatccgg acagatctgt aaatggcggt taaagtcggc acagaacacc
    13501 cagaactccg ccgcttgcgc tacgtgtttt tgcccgccgg tcagcgtcac cagttcttca
    13561 cgtaatgctt tgtcggtaat gcgaataatg ctactacact gcaaaaaact ggaactggac
    13621 gtcgcacggg cgctgttaat aatcgcctca cactgcgctt cggaaatggg ttcatcagtg
    13681 aaatggcgaa tggagcgatg gccacaaata agttcaatgg ttggcgtcat tatctttttc
    13741 tctttctgaa cgtgaatatt gcggtggacg gttcatcagc tgtggggcaa gacgttttgc
    13801 cacctgaaga ataaccacca ccgcagcggg aagcatgagc aaaacaccga gaaaaatcat
    13861 cagaatctgc acttctggcc gagaaaatgg ctcaggcagc gacagggagt cgcttaccga
    13921 cagcagcgcc accgccagta gcatcattcc gataaattcc agtatcaaca cgcctttagg
    13981 caatttaccg atcgcgcgca tacgcttccc tctgcaaagt gagccttcag tctaaaactt
    14041 ttcactgtat tgtgtttaac agttatagct tttagcaatt aatgcaacag gttaaaccta
    14101 ctttcagcga atacatttta gcgtgatcat tacaggcata aatctatgag gagagaaata
    14161 atgcaaaccg ttatttttgg tcgttcgggt tgcccttact gtgtgcgtgc aaaagatctg
    14221 gctgagaaat tgagcaatga acgcgatgat tttcagtatc agtatgtaga tattcgtgcg
    14281 gaagggatca ctaaagaaga tctacaacaa aaggcaggta aacccgtaga aaccgtgccg
    14341 cagatttttg tcgatcagca acatatcggc ggctataccg attttgctgc atgggtgaaa
    14401 gaaaatctgg acgcctgatc gtctgacaag ccctcgcgtt gagggcttta ctgatttttt
    14461 ctgtgctgtg gtttaaacaa actactgata aataagaaac acagtgcccc cagcgcacac
    14521 cagaacaccg cgcttagtaa ccatgccagc tcttgccaga atgagcgcgt cggtgaaaaa
    14581 aacagccgca taatgagcat tgaacagggt gccgccagca ttgcgccaaa tagaggtttc
    14641 aggacttctc tacgctgtga aaagaaactg gcaactgctc caggaagaat gaaaaacagc
    14701 aagccgattt caggatgccc ggcagcccga aaagcgcctt tcatgtgcgt cgccagaaaa
    14761 aggcacacca caatgaagag gacaaaacag cagattgccc ccgcccaacg ttgtttatgt
    14821 ttcactcgtt cctcctgaca ctgcgtctat cgaacacatt tttcgccagt gtggcgttca
    14881 gtaagataaa gccgcttcgc attccatgct aatataggcc aacgcaattc atatagccgt
    14941 tgatacctaa tgtgattaca ctagtaaaat atattgttac tttactatcg tttaggtgcg
    15001 ctgaatgaat ctgcgccctg aattctggta aaaaacatta tcgtaaatta ccatttcttt
    15061 caacagctta ctagtaaaca agaagttagc ctccgtgaat ataaacgtcg ccgaattgtt
    15121 aaatgggaat tacattctgt tattatttgt ggtcctcgcg cttgggctat gtctcggaaa
    15181 gttacgactt ggttcgatcc aactgggtaa ttccattggc gttttagtcg tatcgctgtt
    15241 attaggccaa caacatttca gcattaacac cgatgcgctt aatcttggct ttatgctgtt
    15301 tattttctgc gtcggggtcg aagccggacc gaactttttt tccatttttt ttcgcgatgg
    15361 gaaaaattac ctaatgttag cactggtgat ggttggcagt gcgctggtga tcgccttagg
    15421 gttaggtaag ctgtttggct gggatattgg cctgacggcc ggtatgttag caggctctat
    15481 gacgtcgaca ccggttctgg tcggtgctgg cgatacactg cgtcattccg gcatggaaag
    15541 caggcagctc tcactggcac tggataatct gagcctcggg tatgccttaa cctatttaat
    15601 cggtctggtg agtttgattg ttggtgcgcg ttacttgccg aaattgcagc atcaggactt
    15661 acagaccagc gcccagcaaa tcgcccgcga acgtggcctg gacactgatg ccaaccgtaa
    15721 ggtttattta ccggtgatcc gcgcctatcg cgtcggcccg gagctggtgg cctggaccga
    15781 cggcaaaaat ctgcgtgaac tgggtattta tcgacaaacc ggctgctaca ttgaacgtat
    15841 tcgacgtaac gggattctgg caaatccaga cggtgatgcc gtgctacaaa tgggcgatga
    15901 aatagcgttg gtaggctatc ccgacgccca tgcccgactc gatcccagct tccgtaacgg
    15961 taaagaagtt ttcgatcgtg accttctcga catgcgtatc gtcactgaag aagtggtcgt
    16021 taaaaaccat aacgctgtag gtaaacgtct cgcacaactg aagttgaccg atcacggttg
    16081 cttccttaac cgcgtcattc gtagccagat tgagatgccg atagatgaca acgtcgtgct
    16141 taacaaaggt gacgttttac aagtcagcgg tgatgcccgt cgcgtaaaaa ccatcgccga
    16201 tcgcatcggc tttatctcga ttcacagcca ggtcactgac ctgctggcat tttgcgcctt
    16261 ctttgttatt gggctgatga tcgggatgat caccttccag ttcagcacat tcagtttcgg
    16321 catggggaac gctgccgggt tgttattcgc cggaattatg ctgggcttta tgcgtgctaa
    16381 ccacccgacc ttcggttaca ttccgcaagg tgcattaagc atggtgaaag agttcggctt
    16441 gatggtgttt atggcaggcg ttggtctgag cgccggtagc ggtattaata acggcctggg
    16501 cgcgattggc ggtcagatgt tgattgccgg attaattgtc agtcttgtgc ccgtggttat
    16561 ctgtttcttg ttcggtgctt atgtattgcg aatgaaccgc gcactgttgt tcggcgcaat
    16621 gatgggcgca cgcacctgcg cgccggcaat ggagatcatc agtgatacag ctcgcagtaa
    16681 catccctgcg ctgggctatg cgggcaccta cgcaatcgcc aacgtcctgc tgacgctggc
    16741 agggacaatc atcgtcatgg tatggccagg attaggataa aactgaagtt gccctgaaaa
    16801 tgaaattttt ttgcacaacc gcagaacttt tccgcagggc atcagtctta attagtgcca
    16861 ctgcttttct ttgatgtccc cattttgtgg agcccatcaa ccccgccatt tcggttcaag
    16921 gttgatgggt tttttgttat ctaaaattta tctactaccc tgcaaccctc tcaaccatcc
    16981 tcaaaatctc ctcgcgcgat agcggcttac ggtcggtgac aaaatgcagc gtcatcccct
    17041 caataaacgc atcaagcgcg cgggcggttc cgggttcaaa ccattgttcg agcgtttgct
    17101 gactgcgctg catccagttt tgcattaccg tttttaatag cggttttcgg ctagccagcg
    17161 cgtagagctg gtacatcagc tccatattat ccggcgttgc aacctgtgag ctgtagatca
    17221 tatcggtgat agcctggcat gcgccttgag catcactaac atcgctaaaa aatgcctgat
    17281 attgccggga cataatctca gtaaaactgc tgaacgcctc cagcaacaac tcatcaattc
    17341 ctgaaaaata gtaggtcatc gatcccaacg gtaccccggc aagggtagca attttgcggt
    17401 gcgtaacagc atgtattccg taaagtttca ccgcctccag cgtggcctgg ataatttttt
    17461 ctcgccgttg cggatcgtta gcgcgacgca tgtcatttcc tctgtctgca attgtgtaca
    17521 aatgtacaca accttgctaa tgttgtcttc cctcttctta ttttgacgtg atctatgacc
    17581 gtaaattctt cacgtaatgc attgaaacgc cgaacctggg cgctgtttat gttcttcttt
    17641 ttgccaggcc tgttaatggc gtcctgggca acccgtacgc ctgctatccg cgatattctc
    17701 tctgtctcga tcgctgaaat gggcggtgtt ctctttggtc tgtcgatcgg ttcgatgagc
    17761 ggtattctct gctcggcgtg gttagtgaaa cgctttggga cacgtaatgt catcctggtc
    17821 acgatgtcct gcgcattgat cgggatgatg atattaagtc tggcactctg gctgacatcg
    17881 cccctgctct ttgccgttgg tctcggcgtc tttggggcaa gttttggttc tgcggaagtg
    17941 gcgataaacg ttgaaggtgc cgccgttgag cgagaaatga ataaaacggt tttgccgatg
    18001 atgcacggtt tttatagcct aggcacgctg gcaggcgctg gtgtcgggat ggcactgacg
    18061 gcctttggcg ttccggcaac ggtgcacatt ttattggcgg cgctggtagg tatcgcgcct
    18121 atttatatcg ccattcaggc aatccctgac ggtacgggca aaaatgctgc cgatggcacc
    18181 cagcatggcg aaaaaggcat tcctttttat cgcgatatcc agttgctgtt gattggtgtt
    18241 gtagtgctgg cgatggcctt tgccgaaggt tctgccaacg actggttacc cttattaatg
    18301 gttgacggtc acggttttag ccctacttcc ggctcgctga tttatgccgg ttttacccta
    18361 gggatgaccg ttggacgctt cactggcggt tggttcatcg accgttacag tcgcgttgcc
    18421 gtggttcggg ccagtgcact aatgggggcg ttgggtattg ggctgattat ttttgtcgat
    18481 agcgcctggg tcgctggggt gtctgttgta ctctggggac tgggtgcctc gctgggcttc
    18541 ccgctgacca tttctgccgc cagcgatacc ggccccgatg caccaacccg cgtcagtgtg
    18601 gtagcaacga ccggttatct ggctttcctc gtcgggccgc cgctgctggg ctatctcggc
    18661 gaacattatg gattacgtag tgcaatgctg gttgtactgg cgctggttat tctcgcggct
    18721 attgtcgcga aagccgtcgc caaacccgat accaaaacgc ggacggcgat ggagaatagt
    18781 tgatgagcat taaattaatt gcggtagaca tggatggtac tttcttaagc gatcaaaaaa
    18841 cctataaccg tgagcggttt atggctcagt atcagcaaat gaaagcacaa ggcattcgct
    18901 ttgtggtcgc cagcgggaat caatattatc agttgctctc tttcttccct gaaattgcta
    18961 atgaaattgc ctttgtggct gaaaacggcg gctgggtagt gagtgaaggc aaagatgttt
    19021 ttaatggcga gctgtcgaag gatgccttta ctactgtcgt ggaacatttg ctgacgcgcc
    19081 cggaagtgga aattattgcc tgcggaaaaa atagcgccta tacgctcaaa aaatatgacg
    19141 atgccatgaa aacggtggca gaaatgtatt atcaccgtct ggaatacgtc gataactttg
    19201 acaacttaga agatattttc tttaagtttg gcctgaatct ttccgatgaa ctgattccac
    19261 aagtacaaaa agcattacat gaggccatcg gcgatattat ggtgccagtc cacaccggca
    19321 acggcagcat cgatctgatt atccccggcg tacataaagc caatggcctt cgccaactgc
    19381 agaaattatg gggaatagac gacagcgaag tggtggtctt tggcgatggc ggtaacgata
    19441 ttgagatgct gcgtcaggca ggctttagtt ttgcaatgga aaatgccggc agcgcggtcg
    19501 tcgcagcggc aaaataccgg gcaggctcca ataaccgtga aggcgtactg gatgtgatcg
    19561 ataaagtgct taaacacgaa gcgccatttg accaataaaa acggcagcac gcccggcaac
    19621 acaatacctg aatgaaggct gcataattcg cggaaattaa cccgctgcat tatttcaggc
    19681 gaaatgatta tgaaaaattg tctactgttg ggcgcgcttt taatgggctt tactggtgtg
    19741 gcgatggcgc aaagtgtcac cggcgatgtg ccgagtggat acaaagtggt tgtagttcct
    19801 gattcagtca gcgttccaca agcggtcagc gtcgcgactg tgccgcaaac cgtctatgtg
    19861 gctcctgcgc cagctccggc ttaccgcccg catccttatg ctcgccatct tgccagcgta
    19921 ggtgaaggga tggttatcga acaccagatt gacgaccatc accattaatt cgagaatgcc
    19981 tgatcgcaca aagcagtcag gcattttttt acccttcgtg agaatttccc atctgtttat
    20041 cttttaaaaa gataaccatc agcgacagcc acaaaattcc gttgacaagg ttgaagagat
    20101 taaactgtcc gttgcccccg ttcagccagg catgtttgct gatttcaata ccaacggtaa
    20161 agatcagcat ttgcagcatt cccatcgccg cagaaaccgt acctttactc atatcgctgg
    20221 caaacagggt taatcgcacc agtcccgcat tcgccagacc aataccgaaa gcataaatac
    20281 ttaacccggc agtcatccat aaatacgcgt gcgatgagat aaccgttgcc gcagcagcga
    20341 ccaacagacc aatcataatc ggccagccgc ccataattat cacgtacggt gcggcgcgag
    20401 gtcagacgcg ctaacagcaa gttacccgca attaacgccc cgaaaatagg cacttgcagc
    20461 aagccatatt catagctgct caactgctcc ccggtaatga tgataatcgg cgactgggcg
    20521 atccacgcca gcaatggcag gctaacgaat cccagcgcca gcgcccccgc cacaaagcgg
    20581 ccgttcttca gcaccagctt atagtcacga ccgagttctt tcagtgacag tttctcgcct
    20641 atacgcgtgg cggtttcagg catagctcgt tgcagaccga aaaaggagat cgctgccaat
    20701 gcggcaaaca aaacaaacat cccctcccag ggcagcacat ggatccacgc cgcgcccacc
    20761 agcggaccaa gtagcggagc aatcagcgcc acgttcgcca tcagcgcggt gatcttgata
    20821 caaaccgcct cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc aatgaaacag
    20881 aggcttatgc cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg cgccagtaat
    20941 attgccagac aggtgacgat aaaccacacc acacccgcca gcatcaccgg acggcgacca
    21001 atacgatccg acagcggccc caggagccat tgtaaaaaca tcccgcccgc cagatacgcg
    21061 gtcatcgaag taggaaccca atcaatgccc gcctgatatt gttccaccac ggccaacata
    21121 ccgggttgga tcatatcgtt gccgatatag gttgaaaatt cgtaaagcac cagacagaga
    21181 gggaaaagta acgcctgacg tccaagcctg gcaccgaaag ctaatttatt ttgcatgcaa
    21241 tttcttcgcc aataataatc gcgcagagtt taataaaagc gcagctaacg agaaagcgaa
    21301 ttttgtagct gaaaccacgg ttaagcacat tcttacatta ttgcgagtat agctacgctt
    21361 tctttaagtt ttatttaacc tatgcccgtt acaatcaccc accgtaaaca ggccgcttga
    21421 gggaaataag acgatgccgc tttacccagt ttaacctgca ctttattctc aacgacttgc
    21481 ctgtattggc tcccttttaa tcactttgcg tcgggaagtt atgctggaaa atttgaatct
    21541 ctctctattc tctcttatta acgcgacgcc agactcggct ccgtggatga tctcgttggc
    21601 gatttttatt gctaaagatt tgattaccgt ggtgccgttg ctggccgcgg tactttggtt
    21661 gtgggggctt acagcacaac ggcaactggt gataaaaatc gctatcgcgc tggcggtcag
    21721 cctgtttgtg tcctggacga tgggacatct ttttccgcac gaccgaccct ttgtcgaaaa
    21781 tatcggctat aacttcctgc atcatgcggc ggatgactca ttcccgagcg atcacggtac
    21841 ggtgattttc acctttgcac tggcattttt atgctggcat cgcctgtggt ccggctcact
    21901 tttaatggtt ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg tccactggcc
    21961 gctggatatg ctcggtggat tgctggcagg tatgattggc tgccttagtg cccagattat
    22021 ctggcaagcg atggggcata aactctatca acgtctgcaa tcgtggtatc gcgtctgttt
    22081 tgcattaccg atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa gtgtagtatt
    22141 gagcggctcg cttcaataac tattcagagg gattatggaa acacgtcgcg aagagcgtat
    22201 cgggcagctg ctgcaagaat taaaacgcag cgataagtta catcttaaag acgccgccgc
    22261 cctgcttggg gtttcggaga tgacgattcg tcgcgatctg aacaaccaca gtgcgcccgt
    22321 cgttttgctc ggcggctata ttgttctgga accgcgcagt gccagccatt acctgttaag
    22381 cgatcaaaaa tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg ctgcgacact
    22441 ggtagaaccc gatcagaccc tcttttttga ctgtggcacc accacgccgt ggattattga
    22501 agcgattgat aatgaaatcc cttttaccgc cgtttgttat tcgctaaata cctttctggc
    22561 gctgaaagag aaaccccatt gccgcgcatt tctgtgcggt ggtgaatttc acgccagcaa
    22621 cgccattttc aaccccatcg attttcagca aacgttgaat aatttttgcc cggatatcgc
    22681 tttttattcc gcggcgggcg tgcatgtcag taaaggcgct acctgtttta atcttgaaga
    22741 gttgccggta aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg ttgtcgacca
    22801 cagtaaattt ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct ttgatattgt
    22861 ggtgagtgat tgttgcccgg aagatgagta tgtgaagtac gcgcagacgc accgtattaa
    22921 gttgatgtat taatgacgta taaccggatg acgtttcgcg ccatccggtt atcagaagat
    22981 taagagaacc agctgccgaa ccactgatgg aatttcatca tcacgaaatc ccacatccga
    23041 ccaaagaatc cgccctcttc cacattttcc atcacgatca gcggacgctg ctcaatggat
    23101 ttaccgttaa gctggaaatc aatggtcccg acaacctgac cttttttcag cggtgcggta
    23161 agctgcggtt ccgttaacgt gtagctcgcc ttcaggtttt tcagctgccc acgcggtatg
    23221 gtcacggagc ccgcttcgcc tgccccgaga ttcacttcgc tcttatcacc aaaccagacg
    23281 cgctgagtca caaaggtggc atcaggttta attggcgtca cagtttcaaa gaagcggaaa
    23341 ccccaggtca ataatttctc agactcatta aaacggatac ggtcagtttt cgcccccagc
    23401 actacggaga ttaaacgcat atcaccctgg gtagccgaag caaccagatt atatcccgca
    23461 cctgccgtgg ttcctgtctt catgccatca acattcagat tgctgctcca cagcagacgg
    23521 ttacggttag gctgacgaat tttgttgaag gtgaactctt tctctttatg aatggcgtac
    23581 tcttccggca catcgtggat caatgcttta cccagcaatg ccatatcgcg cgcggtactg
    23641 aactgccccg gcgcatccag accgtgcacc gtctggaagg tagtgttggt cagacccagt
    23701 tttttggcat aaccattcat caaaccaata aatgactcct ggctcccggc aacgtaatcg
    23761 gccagcgcaa tacaggcgtc attaccggac tggataatca cacctttgtt caagtctgcc
    23821 accgaaacct gatcgcccgg tttgaggaac attaccgatg aaccacgcag tgccggattt
    23881 cccgtcgccc aggcatcttt accgaccgtc accatatcgg tgagtttaat cttatcggcc
    23941 ttaagcgcct gcccaaccac atagctggtc atgattttag tcaggctcgc gggatccagt
    24001 ttctcatccg cgttgccttc tgccagcact ttaccgctgg cgtaatccat taaaatccat
    24061 gcacgcgcat ccacgctcgg cgcttcaacg gtttgttccg ccgcgaatgc cgttggggca
    24121 aaaaggaata aaaatgcaga acccgctgca agaccacgaa ggagagagga gtattgcgtc
    24181 ataaatgcca cccgagtatc cattcaaaaa aaatacgtta tatcgccgta tgataagttc
    24241 ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag tttttaaagt
    24301 ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag ctgttgctta
    24361 aaagttaact ttacttcacc atcggagtcc tgttcagcct gtggctgcct gtcggaaaag
    24421 gggtgagtat gattacgctg tggggtcgga ataattcaac taacgtaaaa aagtattgct
    24481 gacgctcgaa gaactggaac taccttatga gcaaattctc gcgggccgtg agtttgggat
    24541 aaatcacgat gctgattttc tggcgatgaa ccctaacggg ctggtgccgt tgttgcgtga
    24601 cgacgaaagt gatctcattc tttgggaatc aaacgccatt gtccgctatc tggcggcaca
    24661 gtacgggcaa aaacgcctgt ggatcgactc accggcacgt cgtgcggaag cggaaaaatg
    24721 gatggactgg gcaaaccaga cgctcagtaa tgctcatcgc gggatcctga tgggattagt
    24781 cagaacacca ccggaagagc gcgatcaggc cgccattgat gccagttgca aagagtgcga
    24841 cgccctgttt gccctgctcg atgcggaact ggcaaaagta aaatggttct ctggcgacga
    24901 gtttggtgtg ggcgatatcg ctatcgcacc gttcatctac aatttgttta acgttggcct
    24961 gacctggaca ccgcgtccaa atctgcaacg ctggtatcag caactcactg aacgccccgc
    25021 agtgcgcaaa gtggtgatga ttcccgttag ctaattgcgt gggctaactt taagtaattc
    25081 cccactggac tcgtcggtga gaacgtataa ataaccgtcg ggtccagtgc gaacatcacg
    25141 aattcgctgc cctctgtccg ttaaaatacg gccatcttct gtcactttgt cgccgttgac
    25201 gctcatcaca atgacatctt tatctttcag cgcgccaata aataattttt gctgccactg
    25261 ggggaattta tcgctgttat agaaggccat gccgctcaca gcgggcgaat ctttccagta
    25321 aaaaacaggt tgctcggtcc cggcgacgat ctcccctttc gcttccggta tcttaaagcc
    25381 tgaatagttg attccccagg ttgccagcgg ccagccgtag tttttgcctt tttgcgggat
    25441 attaatttca tcaccaccgc gcgggccatg ttcattcagc cacagtgcat tactccacgg
    25501 attcatcgcc attccttgcg gattacgaat gccataagac cagatctcgg cgcgcgcacc
    25561 ggattccttt ataaaaggat tatcatccgg gatttcgccc tggtcggtca gacgcaccag
    25621 tttgccctgt aatttatcca gatcctgcgc cgtcgggcgc tgattgtttt cgcccagagc
    25681 aataaaaaga taacctttac cgtcgaatac cagccgcccg ccaaaatggt tgccggtaga
    25741 cagttttggc atctggcgga agacggtgcg gaagtcggtc acttttgaga gatcatcact
    25801 taagcggcca taacccacag cagttccggc tttgccatca tcgccaactt cggaataact
    25861 taaccagatg cggcgagact gagcaaaatc aggcgctaaa accacgtcca gcaggccgcc
    25921 ctgcccgtgc gcccaaacgt ccggaactcc ggaaagcggc gcagataatc cttttcctgc
    25981 ttgccagtga cgcaactcgc cgccgcgcag agtgattaac ataccgtgat tatcgggtaa
    26041 aaaggccagt gcccagggat ggtcgagttt gtcttgcagt acttcgacat ttaccgttgc
    26101 aggagccgcc cagagagcgg aagaaagaca aataaggggc acaaggaaaa aggattgtcg
    26161 atgcataacg cctccggatt gactctggct taaagcgtag tcagtggagg agataacaaa
    26221 ttcattttta caaaaactta aacatgaagg gggagacgct ttctccccct tagttttcag
    26281 gccttctcaa gcatggcgtg cttctgcagg ctctggatac tcagcgttaa gctcatcaga
    26341 caattttcaa gcttatcggc gttgacggta ataacagtcg ggcaatcatg gtgcccactc
    26401 atcaaacata ctgcggctgt cgctaatgct tcttcagcat gatgaagagc actccactct
    26461 tcctgatcca gatgaagatt caaccgcagc gatttatcgt gcagttcgcg attcagttta
    26521 aaaaagttat ctcgtagatg attgctttcg ctgacggaca tgtatccttt tgccttcctc
    26581 agttgcacgt atgcagcgag gtcaacgcgt gcgtcgatca accggttcag cagatcgatt
    26641 cgctgtctgt caacgaacat acttcgatcc tcctctttcc agccttgttg cgattaaata
    26701 gtatggctat tttttgagca aaatgctgtc caggatcaat tcttcacgcc aaattaacaa
    26761 aatttacatt tccttcgcct ccgccagcga atgagattgc gatacaaatt ttatccagaa
    26821 ctctgtagaa tccctgccct gacctttcct gtaactgaat attttttaag ctatgagcaa
    26881 agtaactccc cagccgaaaa tcggctttgt ttcccttggc tgtccgaaaa accttgtcga
    26941 ttcagagcgt attctcaccg aactacgcac tgaaggttat gacgtggtac cgagctatga
    27001 cgatgcggac atggtgatcg tcaacacctg cggctttatt gacagcgcgg tacaagaatc
    27061 actggaagcc attggtgaag cgttgaatga aaacggcaag gtaattgtga ccggttgtct
    27121 gggggcaaaa gaagatcaga tccgcgaagt ccacccgaaa gtgctggaaa tcaccgggcc
    27181 tcatagctat gagcaggttc tggagcacgt tcatcactac gtgccaaaac cgaaacacaa
    27241 cccattcctg agcctggtgc cagaacaagg tgtgaaactg acaccgcgtc attatgccta
    27301 tctgaaaatt tcggaaggct gtaatcaccg ctgcaccttc tgcattattc cgtctatgcg
    27361 cggcgacctg gtgagccgtc cgattggcga agtgttaagt gaagcgaaac gtctggtaga
    27421 tgcgggcgtt aaagagattc tggtgatctc gcaggatact tccgcctatg gcgttgatgt
    27481 taaacatcgt actggcttcc acaacggcga gccggtaaaa accagcatgg tcagcctgtg
    27541 cgaacagtta tcgaaactgg ggatctggac acgtctgcac tacgtttacc cttatccgca
    27601 tgtggacgac gtcatcccac tgatggcaga aggcaaaatc ctgccgtatc tggacattcc
    27661 gttgcagcac gccagcccgc gcattctcaa actgatgaag cgtccgggtt ctgtagatcg
    27721 ccaactggcg cgcatcaaac agtggcgcga aatctgcccg gaactgaccc tacgctcaac
    27781 ctttattgtc ggcttcccta gcgagacgga agaagatttc cagatgctac tcgacttcct
    27841 gaaagaagcg cgtctggatc gcgttggctg ctttaaatac agcccggttg aaggtgcaga
    27901 cgccaatgcc ctgcctgacc aggttccgga agaagtgaaa gaagaacgct ggaaccgttt
    27961 catgcagctg cagcagcaga tttccgccga gcgcctgcaa gagaaagtgg gccgtgaaat
    28021 tctggtgatt atcgacgaag tggacgaaga aggcgcgatt ggtcgcagca tggcagatgc
    28081 accggaaatc gacggcgcgg tttatctcaa tggtgaaacc aacgttaagc cgggtgatat
    28141 cctgcgtgtg aaagtcgagc acgccgatga gtacgatttg tggggtagcc gggtttaatt
    28201 aattcaggcc gggcaaaatc atttgtccgc cctgcgaata atgattactg ccagctaatc
    28261 tgagccctgc cgtttttctt actcttatac agccgctcat ccgccagcat tttcaagcgt
    28321 tcgacggtgg tacattcatt tgaatgcgct acaccgatac tggtacttaa attaatgctt
    28381 cccacctgga aatgaacgat ttcttttaga atcgatagca actcttccag cgttccaccc
    28441 gtataaatca ccgcaaattc gtcaccgccg aagcggtaga ttttaccttt atctaccggg
    28501 atctgtgatt taaggatttt caccactttc atcaacactt catcgcccag caaatgaccg
    28561 aaggtgtcgt taatgttttt gaatttatcg acgtcaaaca ggcacaaata accgcctttt
    28621 tccggcagcg cttttaaatc ttcgtcaaag gattttctgc ccattgcttt ggtcaggtcg
    28681 tccaggaacg ccgagttata catactgtag agttttttga catcggcagc cgaaaagaaa
    28741 agcccgccat ccagtacgcg ttcaaaatgg ttattaatga tggcttcttt ctccacaata
    28801 ccacgattaa tcactctgcg taccagatag aggcaactgg tacccgatat cactgccacc
    28861 aatgcggcaa agaaaataat caatgtacag acccgcaaat aaacatcttt tttatacgca
    28921 aatacgtaca atccggtcgg caaataggcg aagtgctcca tcgcttccga aacgatgaag
    28981 atggcgtttt tagcattata ttcatcaccg gtttaccgtt cttaaataga gagaacatca
    29041 ggttctcgct gtccatcgcc agcgattttg acagtaaaga aaaacgctcc agaccaagca
    29101 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
    29161 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
    29221 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
    29281 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
    29341 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
    29401 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
    29461 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
    29521 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
    29581 tttactaatt ttccactttt atcccaggcg gagaatggca tgggtttctg gtacagatac
    29641 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
    29701 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
    29761 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
    29821 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
    29881 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
    29941 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
    30001 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
    30061 tgcagtaaga aatcatcctt cagcgacgca cgctgactaa aatgtctgaa ttcacccatt
    30121 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
    30181 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
    30241 gcgcccagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
    30301 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
    30361 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
    30421 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
    30481 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
    30541 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
    30601 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
    30661 ctgacgattt tattgcgttc atcaacacca aacaccgctt ccacggcttc cagaccacac
    30721 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
    30781 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
    30841 atttcggcat ctttcatttc cgcgatcgaa
//
LOCUS       DAZOZY010000027        30154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZOZY010000027 DAZOZY010000000
VERSION     DAZOZY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.027"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHURQ_001094"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHURQ_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR0822929.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHURQ_001095"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHURQ_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR0822930.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHURQ_001096"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHURQ_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR0822931.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHURQ_001097"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHURQ_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR0822932.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHURQ_001098"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHURQ_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR0822933.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHURQ_001099"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHURQ_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR0822934.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHURQ_001100"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHURQ_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR0822935.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHURQ_001101"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHURQ_001101"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR0822936.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHURQ_001102"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHURQ_001102"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR0822937.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHURQ_001103"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHURQ_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR0822938.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHURQ_001104"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHURQ_001104"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR0822939.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHURQ_001105"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHURQ_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR0822940.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHURQ_001106"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHURQ_001106"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR0822941.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHURQ_001107"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHURQ_001107"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR0822942.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHURQ_001108"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHURQ_001108"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR0822943.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="ACHURQ_001109"
     CDS             complement(18516..18992)
                     /locus_tag="ACHURQ_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR0822944.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHURQ_001110"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHURQ_001110"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR0822945.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPLSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHURQ_001111"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHURQ_001111"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR0822946.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHURQ_001112"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHURQ_001112"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR0822947.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHURQ_001113"
     CDS             21688..22278
                     /locus_tag="ACHURQ_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR0822948.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHURQ_001114"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHURQ_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR0822949.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHURQ_001115"
     CDS             22750..23445
                     /locus_tag="ACHURQ_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR0822950.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHURQ_001116"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHURQ_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR0822951.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHURQ_001117"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHURQ_001117"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR0822952.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHURQ_001118"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHURQ_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR0822953.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHURQ_001119"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHURQ_001119"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR0822954.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHURQ_001120"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHURQ_001120"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR0822955.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7056 a         7867 c         8307 g         6924 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tccttgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctctg
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaag
//
LOCUS       DAZOZY010000028        29549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZOZY010000028 DAZOZY010000000
VERSION     DAZOZY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.028"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..690
                     /gene="dgt"
                     /locus_tag="ACHURQ_001121"
     CDS             <1..690
                     /gene="dgt"
                     /locus_tag="ACHURQ_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR0822956.1"
                     /translation="LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSR
                     SNSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDL
                     LKLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKR
                     FPIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAW
                     DEYRRLMAVEQ"
     gene            820..2244
                     /gene="degP"
                     /locus_tag="ACHURQ_001122"
     CDS             820..2244
                     /gene="degP"
                     /locus_tag="ACHURQ_001122"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR0822957.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2399..3556
                     /gene="cdaR"
                     /locus_tag="ACHURQ_001123"
     CDS             2399..3556
                     /gene="cdaR"
                     /locus_tag="ACHURQ_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR0822958.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3645..4031)
                     /locus_tag="ACHURQ_001124"
     CDS             complement(3645..4031)
                     /locus_tag="ACHURQ_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR0822959.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="ACHURQ_001125"
     CDS             complement(4193..5005)
                     /gene="yaeI"
                     /locus_tag="ACHURQ_001125"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR0822960.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="ACHURQ_001126"
     CDS             complement(5060..5884)
                     /gene="dapD"
                     /locus_tag="ACHURQ_001126"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR0822961.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="ACHURQ_001127"
     CDS             complement(5915..8587)
                     /gene="glnD"
                     /locus_tag="ACHURQ_001127"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR0822962.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8649..9443)
                     /gene="map"
                     /locus_tag="ACHURQ_001128"
     CDS             complement(8649..9443)
                     /gene="map"
                     /locus_tag="ACHURQ_001128"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR0822963.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9811..10536
                     /gene="rpsB"
                     /locus_tag="ACHURQ_001129"
     CDS             9811..10536
                     /gene="rpsB"
                     /locus_tag="ACHURQ_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR0822964.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10794..11645
                     /gene="tsf"
                     /locus_tag="ACHURQ_001130"
     CDS             10794..11645
                     /gene="tsf"
                     /locus_tag="ACHURQ_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR0822965.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11792..12517
                     /gene="pyrH"
                     /locus_tag="ACHURQ_001131"
     CDS             11792..12517
                     /gene="pyrH"
                     /locus_tag="ACHURQ_001131"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR0822966.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12809..13366
                     /gene="frr"
                     /locus_tag="ACHURQ_001132"
     CDS             12809..13366
                     /gene="frr"
                     /locus_tag="ACHURQ_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR0822967.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13458..14654
                     /gene="ispC"
                     /locus_tag="ACHURQ_001133"
     CDS             13458..14654
                     /gene="ispC"
                     /locus_tag="ACHURQ_001133"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR0822968.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14843..15601
                     /gene="ispU"
                     /locus_tag="ACHURQ_001134"
                     /gene_synonym="uppS"
     CDS             14843..15601
                     /gene="ispU"
                     /locus_tag="ACHURQ_001134"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR0822969.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15614..16471
                     /gene="cdsA"
                     /locus_tag="ACHURQ_001135"
     CDS             15614..16471
                     /gene="cdsA"
                     /locus_tag="ACHURQ_001135"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0822970.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16483..17835
                     /gene="rseP"
                     /locus_tag="ACHURQ_001136"
     CDS             16483..17835
                     /gene="rseP"
                     /locus_tag="ACHURQ_001136"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR0822971.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17865..20297
                     /gene="bamA"
                     /locus_tag="ACHURQ_001137"
     CDS             17865..20297
                     /gene="bamA"
                     /locus_tag="ACHURQ_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR0822972.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20419..20904
                     /gene="skp"
                     /locus_tag="ACHURQ_001138"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20419..20904
                     /gene="skp"
                     /locus_tag="ACHURQ_001138"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR0822973.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20908..21933
                     /gene="lpxD"
                     /locus_tag="ACHURQ_001139"
     CDS             20908..21933
                     /gene="lpxD"
                     /locus_tag="ACHURQ_001139"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR0822974.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22038..22493
                     /gene="fabZ"
                     /locus_tag="ACHURQ_001140"
     CDS             22038..22493
                     /gene="fabZ"
                     /locus_tag="ACHURQ_001140"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR0822975.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22497..23285
                     /gene="lpxA"
                     /locus_tag="ACHURQ_001141"
     CDS             22497..23285
                     /gene="lpxA"
                     /locus_tag="ACHURQ_001141"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR0822976.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23285..24433
                     /gene="lpxB"
                     /locus_tag="ACHURQ_001142"
     CDS             23285..24433
                     /gene="lpxB"
                     /locus_tag="ACHURQ_001142"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR0822977.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24430..25026
                     /gene="rnhB"
                     /locus_tag="ACHURQ_001143"
     CDS             24430..25026
                     /gene="rnhB"
                     /locus_tag="ACHURQ_001143"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR0822978.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25063..28545
                     /gene="dnaE"
                     /locus_tag="ACHURQ_001144"
     CDS             25063..28545
                     /gene="dnaE"
                     /locus_tag="ACHURQ_001144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR0822979.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28558..29517
                     /gene="accA"
                     /locus_tag="ACHURQ_001145"
     CDS             28558..29517
                     /gene="accA"
                     /locus_tag="ACHURQ_001145"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR0822980.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7122 c         8160 g         7108 t
ORIGIN      
        1 cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca tcatttgcac
       61 gaggcgtggg gtcagcatga gaaaggttcg ctcttttcgc tagtggttga aaatgcctgg
      121 gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt tatgtattta
      181 cgggtaaaca ccctaaataa actggtaccc tacgcggcac aacgatttat tgataatctg
      241 cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag cgaatgcagc
      301 gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca tccggatgtc
      361 gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat ttatcgtcct
      421 ttattaagcc tgtcgttatc agactttact gaactggtag aaaaagaacg ggtgaaacgt
      481 ttccctattg agtcgcgctt attccacaaa ctctcgacgc gccatcggct ggcctatgtc
      541 gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga atattattac
      601 cgttgccgcc tactgcagga ttatatcagc ggtatgaccg acctctatgc gtgggatgaa
      661 taccgacgtc tgatggccgt agaacaataa ccaggctttt gtaaagacga acaataaatt
      721 tttacctttt gcagaaactt tagttcggaa cttcgcgcta taaaacgaat ctgaagaaca
      781 cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaac cacattagca
      841 ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc aacggcggct
      901 gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat gctcgaaaag
      961 gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa tacgccgcgt
     1021 atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca ggaaggttca
     1081 ccgtttcaga gctctccgtt ctgccagggg ggccagggcg gtaatggcgg cggccagcaa
     1141 cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg ctatgtcgtc
     1201 accaacaacc atgttgttga taacgcgacg gtgattaaag tccagctgag cgatggtcgt
     1261 aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct gatccaaatc
     1321 cagaacccga aaaacctgac cgcaattaag atggcggatt ctgatgcact gcgcgtgggt
     1381 gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac ttccgggatt
     1441 gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt catccagacc
     1501 gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa cggcgaactg
     1561 atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat cggttttgct
     1621 atcccgagta acatggtgaa aaacctgacc tcgcagatgg tggaatacgg ccaggtgaaa
     1681 cgcggtgagc tggggattat ggggactgag ctgaactccg aactggccaa agcgatgaaa
     1741 gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc cgctgcaaaa
     1801 gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat cagcagcttt
     1861 gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac cctgggctta
     1921 ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag ccagaatcag
     1981 gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa caaaggcaaa
     2041 gatcagggcg tggtagtgaa caacgtgaaa acgggcactc cggctgcgca gatcggcctg
     2101 aagaaaggtg atgtgattat tggcgcgaac cagcaggcag tgaaaaacat cgctgaactg
     2161 cgtaaagtgc tcgacagcaa accgtctgtg ctggcactga acattcagcg cggcgacagc
     2221 accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaatacg ggaaggggtt
     2281 ctccttacaa tctgtgaact tcgccacaac tccatacatc ttcatcatcc tttgggcatt
     2341 tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg gaggacttat
     2401 ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta ccatgcgcat
     2461 catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca gcggcgatcg
     2521 tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg gacgagtcgt
     2581 cgatatcgat gacgcggtag cacgtcatct gcacggtgtg cggcagggga ttaatctacc
     2641 gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac cagagaatct
     2701 gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac agtcgcggtt
     2761 gatgcacctg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga acctgattca
     2821 ggctgaggag aatactcccg cacttactga atgggcgcaa cggctgggga ttgatctcaa
     2881 tcaaccgcga gtggtggcta ttgttgaggt cgacagcggt cagcttggcg tggacagcgc
     2941 aatggcggag ttacaacaac tgcaaaatgc gctgactacg cccgagcgta ataatctggt
     3001 ggcaattgtt tcgctgaccg aaatggtggt gttgaaaccg gcgttgaact cttttgggcg
     3061 ctgggatgca gaagatcatc gtaagcgagt tgaacaactg attacccgca tgaaagagta
     3121 cggccagctg cgttttcgcg tttcactggg caactatttt accggtcctg gcagtattgc
     3181 ccgatcctat cgtacggcga aaacgacgat ggtggtgggt aaacagcgga tgccagaaag
     3241 tcgctgctat ttttatcagg atctgatgtt acctgtgtta ctcgacagtt tgcgtggcga
     3301 ctggcaggcc aacgaactgg cgcgaccgct ggcgcggctg aaagcgatgg acaataatgg
     3361 cttgctgcga cgaacgctgg cggcgtggtt tcgccacaat gtgcaaccgc tggctacgtc
     3421 aaaggcgctg tttattcatc gtaatactct ggagtatcgg cttaatcgta tatcggaact
     3481 gaccgggctt gatttgggta attttgatga caggttgctg ctgtatgtgg cgttacaact
     3541 ggatgaagag cggtaggtta tgcgttaagg tcgaggcggc gctggctcat tcccctcagc
     3601 ccgaagggta gaggggaatg tccgagttaa ccgtcagttc ggcattattt acgcgttagt
     3661 ttttccagat cggcttcaat ctcgctgatc ttattggtta cgactgactc caggtgacgt
     3721 aagtcgtcga ggatcttacg cttaagatca acttcgctgc ggtcacgctg gcagatttga
     3781 tcaagctcat caatgatata ccgtagattc gggctgattt cctggacttc tttataaccc
     3841 tgacccacac catcagcgac gaccgtttta cgctgacgcg gatatttaaa cttcacgctc
     3901 ttggcgaaaa actcgccttt gtctttctgg aaatagattt tcagaatatc gttgttggct
     3961 tcctgccgga ggctgtaacg atcaatttct tcaggattgg taatacccag acttttcaga
     4021 ttgtcgtaca tagcgttacc tcaaaatgag tcagtaaatt gtgcttattt tagcatttgg
     4081 catggcccgc ccggctcgac tgtgatcgca gaaagccttt ttcttccaga cttctatcat
     4141 ggcgtaaaaa tcaaaaaatt acctgcttta ttgtggtgat aaaattcacg ttctacacca
     4201 gttccagcat cgttacttcc gggcggcagt tcagacgcaa accatacaaa ctgcccacgc
     4261 cacgggttgt gtagatgtgt cgttcgccaa aggcatttaa tccggcgacg taacgtttat
     4321 cttcgacagg ggcaaaaggt tcaccaacca acggtacgcg cagttgcccg ccgtgagtat
     4381 ggccgcacag catcagatcc cagggttcat cacgcatgac ttctttgctg tcgggattat
     4441 gtgccagcat cagtcttggc agatttgctt cgctggcggg aggtggtttg cattgtccgg
     4501 cccataagtc accggtgcct accagttcga attgcctgtt cggcgtggcg atcaccgtag
     4561 cctggttaaa caacaccgtg atgcccactg atttcaacgt ctcgccaatt aagtgatttt
     4621 tttctgtacc aacagggcga tcgtggttgc cgaagcaggc aaacgtcggc gcacattcgg
     4681 caagggggga gaggacatca ctaaacgccg aaaaattcag cggcatatca aataatacgt
     4741 aatcgccgcc cagtaatatc aaatcgggct tttgttctat gccaagagca atcgcgtcag
     4801 aaatcaggct taaaggaaca aaacgagagt aatggagatc ggccagaaaa agaattttga
     4861 atggtgctgc gttgtcttta aaaaaggcga ggcggtgacg gattaattca aaccagccta
     4921 gctcccagta atgcatataa ccgaaacctg agctcgtggc tatcgtcgcg gcagtagcct
     4981 gcaacaatcg gcggcgtgaa atcatcgctc atccctgcaa aaaaaatcgg gcagcgtcgt
     5041 gctgcccgtg tgcatacttt tagtcgatgg tacgcagcag ttcgttaatg ccgactttgc
     5101 cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca gtagaggctg tatttgccat
     5161 cttttgacgg cagattacct gaaacaacca cagaccccgc cggaacgcga ccgtagtgga
     5221 tttcgccggt ttcacggtcg taaatacggg tgctctgacc aatgtatacg cccatggaaa
     5281 tgacggaacc ttcttcgaca atcacccctt caaccacttc agagcgtgcg ccaatgaagc
     5341 agttatcttc aatgatggtt gggttagcct gcagcggttc cagcacgccg ccgatgccca
     5401 cgccaccgga aaggtggacg tttttaccaa tctgcgcaca agaaccgacg gtcgcccagg
     5461 tatcaaccat ggtgccttca tcaacatatg cgccgatgtt gacgtaagac ggcatcagca
     5521 cggtgttacg ggcaataaac gcaccctgac gtaccgccgc tggtggcaca acgcggaagc
     5581 cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt catcggcact ttgtcgaagt
     5641 agcggctttc tgccccttcg atcacctgat tatcattaat acggaaagag agcagcaccg
     5701 cttttttcaa ccactgatgc gtcacccact gaccgtcaat tttttccgct acacgcagtg
     5761 caccggaatc cagcagggcg atcacctgat ttaccgcttc gcgggtaacg gtgtctgcgt
     5821 tggctggcgt gatctcggca cggcgttcaa aagcggtttc aataatgttc tgtaactgct
     5881 gcattgttaa actcttttca tatcagtaaa cacatcaccc tttatcgttt ggattgaggg
     5941 cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt attaagtgca cgccggtcag
     6001 cggtggcaat tatgaataaa tcttctactc gctcgccaat ggttgtaatt cgggcaccat
     6061 gaagcgaaat tcccagatcg gcaaaaattt tcccgactct cgccagcagt cctggctggt
     6121 cgagcgcgat aagctcaagg aacgatttgc ggtcggtatg ggttggcaaa aacgtcactt
     6181 cggtttcaac agtaaagtga cgtagtttag ccggttgacg acgcggctgt ggtggctgcc
     6241 agctactttg tgtcagtacc tgctccagac caaaccgaat aacctcatga cgatctgcgg
     6301 atagcgggct accatcgggt tccagcacga tgaaggtatc catcgccata ccgtcgcgag
     6361 tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg gtctaattcg gcacagacgg
     6421 cggcaaacag ataggggcgg tccgggctcc agataaaaat ctcggtgcct ccacgcgtag
     6481 cctgcgggct aagcaatacc agcggtttgc ttaaatcatg ctgtaataaa tggcgggcat
     6541 gccaggccag ttgatttggg ctatggcgga caaaatagtt agcacgacag cgtgaccaaa
     6601 tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag tagtgccagt gcctggagtt
     6661 ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg cattccgcgt cgtagctgct
     6721 tttcggtggc aaaatagagt tcgcgcaaca gactttgctt ccagctattc cacagcgttt
     6781 cgttggtggc gcaaatgtca gccactgtca ggcataccag atagtgcaga cgattttccg
     6841 tttgaacttc ttcggcaaac tgcttgatga cttcagggtc ctgaatatcg cggcgttgtg
     6901 ctgtcaccga catcagcaag tgctggcgaa ccagccaggc aaccagctgt gtttcgcgtg
     6961 agttcagccc gtggagttct gcaaaatgca ctacatcctg agcaccgaga atggagtggt
     7021 cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc gatgaaaatc agctcagttg
     7081 acggcaggcg cggccagacg tccacacaca acggatggcg ctggcgcgtt tcttcactgg
     7141 caaaactctc cagtttcagc atcacgcgga tagtatgttc atccaccgtg taggcgtgga
     7201 acagatcaaa ctgcatctgc ccgacgatat gcgaccattg cggcatatac gcgccgagca
     7261 cgctatggcg atgcattggc aatagcccgc gccgcaccgc tccggggtga cgcagaatgc
     7321 tcaaaaacag ttttcgtgct tccggaatat tacacagcgg ttgttgcaga tggcgacggg
     7381 catggcgtaa ctggcgcagc gtggtggagt aaatgccggt gatcgcactg ttgcgcacca
     7441 tggtgtagaa catacgcaag atggcttccg gctggcgcat aaatagtgtt tcatcacgca
     7501 ggtcgattag cgtaccgcgt agctgaaact catcgtcgat tggacgtggt ttttcgtcgg
     7561 cgggaagggc gaggatggct tcatcgaaca gttgcagcag catctggttg agttcactga
     7621 cgcggcgtgt aacgcggaag taatccttca tcatccgctc gaccggttcg ttgccttcac
     7681 cactgtaatt cagacgctga gcgacgctaa gttggcgatc gaataacagg cgattatcgt
     7741 aacggctgac gaccagatgc agggcaaagc gaatacgcca cagtatatgc agacattcgt
     7801 ttaattccgc ccgctccgct gaggttaaga agccaaaccc aaccatttca tccagcgatg
     7861 ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg gatatcgcgc aagccgccag
     7921 ggctactttt gatgtctggt tcaagattgt agctggtgcc gtggtaacgc tgatggcgct
     7981 ggttctgttc ttcaactttc gccgcgtaga acttgtcgga aggccagaat ccttcgctaa
     8041 aaatatgttt ttgcagttcg aggaagagcg caacatcgcc aattaataag cgggattcga
     8101 ttaaattggt ggcgacggtt aaatccgata acccttccag catgcactct tcaagcgtgc
     8161 gcacactatg accgacttcc agctttacat cccagagcag cgttaacagc tcgcccactt
     8221 tttgcgcctg atcgtccggg agctttttac ggcttaaaat cagtaaatcg atgtcagaaa
     8281 gtggatgcag ctcgccacga ccgtagccac cgacggcgac caatgccagg tcggcaatct
     8341 ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg gtcgataaac tcggtgcgcg
     8401 cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc atcacccagc caacgctgga
     8461 aagtatcgat atgggctttt atcccaccga cggttaattc atcacggggc cagacgcatg
     8521 gattttgcgg ttgaccgggc agggtgggga gagcggtgtt tgcgtactgt tctggaaggg
     8581 tattcattgt gcgccaccca taagattaaa ttatcgcatt aaaaaagccg gcattcgccg
     8641 gcttcatctt attcgtcgtg cgagattatc gccgggatgg tgtcatcctt gcgtagcgtc
     8701 agaatttcgc agccgttatc agtcaccaca atagtatgct catattgtgc agacaagctg
     8761 cgatctttgg tttttaccgt ccagccatct ttcatggtgc ggatctcttt tttacccgcg
     8821 ttgaccattg gctcgatggt gaacgtcatc ccaggtttca gtacgacgtt ggtttcacgg
     8881 gagtcatagt gcagcacctg cggttcttca tggaagccgc gaccaatacc gtgtccgcaa
     8941 tattcacgaa cgacggagaa gccttctgct tcgacaaact tctgaatcgc cgcaccgatt
     9001 tcgcgcaggt taatgcctgg tttgaccatg cgcagcgcca ggtacaggct ttcttgcgtg
     9061 atgcggcaca gacgttcgcc catgatggtc ggcttaccga cgataaacat tttcgaggta
     9121 tcgccgtgga aaccatcttt gattacggtg acatcaatgt taacgatatc gccatctttc
     9181 agcagcttcg catcgtccgg gataccgtgg cacaccactt cattaataga gatgcaaacg
     9241 gatttcggat agccgtgata gccgaggcag gcagaaaccg cgtgttgttc attaacaatg
     9301 taatcattac agatgcgatc cagctcgccg gtgctgacgc ccggtttaac atacggttcg
     9361 atcatctcca gcacttcggc agccagtcgg ccagcgacgc gcattttttc gatatcttct
     9421 ggggtcttga ttgagatagc cattaattct gtccatcagc gtcggtgata ccgacaatat
     9481 atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt gagaatcatt ctgaatttcg
     9541 ccaaacgtgc cactgaacgt tttctataat agaaaattca acgtctgatg ctgtacacag
     9601 cgccaacaat tattggtgtc catgacgtat ttgtggtata aagcgcgccg gacttccgat
     9661 ccatttcgta tacacagact ggacggaagc gacaatctca ctttgtgtaa caacacacac
     9721 gtatcggcac atattccggg gtgccctttg gggtcggtaa tatgggatac gtggaggcat
     9781 aaccccaact tttatataga ggttttaatc atggcaactg tttccatgcg cgacatgctc
     9841 aaggctggtg ttcacttcgg tcaccagacc cgttactgga acccgaaaat gaagccgttc
     9901 atcttcggtg cgcgtaacaa agttcacatc atcaaccttg agaaaactgt accgatgttc
     9961 aacgaagctc tggctgaact gaacaagatt gcttctcgca aaggtaaaat ccttttcgtt
    10021 ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg ctctgagctg cgaccagttc
    10081 ttcgtgaacc atcgctggct gggcggtatg ctgactaact ggaaaaccgt tcgtcagtcc
    10141 atcaaacgtc tgaaagacct ggaaactcag tctcaggacg gtactttcga aaagctgacc
    10201 aagaaagaag cgctgatgcg cactcgtgag ctggagaaac tggaaaacag cctgggcggt
    10261 atcaaagaca tgggcggtct gccggacgct ctgtttgtaa tcgatgctga ccacgaacac
    10321 attgctatca aagaagcaaa caacctgggt attccggtat ttgctatcgt tgataccaac
    10381 tctgatccgg acggtgttga cttcgttatc ccgggtaacg acgacgcaat ccgtgctgtg
    10441 accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag gccgttctca ggatctggct
    10501 tcccaggcgg aagaaagctt cgtagaagct gagtaataag gcttgataac tcccccaaaa
    10561 tagttcgagt tgcagaaagg cggcaagctc gagaattccc gggagcttac atcagtaagt
    10621 gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg aaatatgaag ggggagagcc
    10681 cttatagacc aggtagtaca cgtttggtta gggggcctgc atatggcccc ctttttcact
    10741 tttatatctg tgcggtttaa tgccgggcag atcacatctc cgaggatttt agaatggctg
    10801 aaattaccgc atccctggta aaagagctgc gtgagcgtac tggcgcaggc atgatggatt
    10861 gcaaaaaagc actgactgaa gctaacggcg acatcgagct ggcaatcgaa aacatgcgta
    10921 agtccggtgc tattaaagca gcgaaaaaag caggcaacgt tgctgctgac ggcgtgatca
    10981 aaaccaaaat cgacggcaac tacggcatca ttctggaagt taactgccag actgacttcg
    11041 ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt tctggacgca gctgttgctg
    11101 gcaaaatcac tgacgttgaa gttctgaaag cacagttcga agaagaacgt gttgcgctgg
    11161 tagcgaaaat tggtgagaac atcaacattc gccgcgttgc tgcgctggaa ggcgacgttc
    11221 tgggttctta tcagcacggt gcgcgtatcg gcgttctggt tgctgctaaa ggcgctgacg
    11281 aagagctggt taaacacatc gctatgcacg ttgctgcaag caagccagaa ttcatcaaac
    11341 cagaagacgt atccgctgaa gtggtagaaa aagaatacca ggtacagctg gatatcgcga
    11401 tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt tgaaggccgc atgaagaaat
    11461 tcaccggcga agtttctctg accggtcagc cgttcgttat ggaaccaagc aaaactgttg
    11521 gtcagctgct gaaagagcat aacgctgaag tgactggctt catccgcttc gaagtggggg
    11581 aaggcatcga gaaagttgag actgactttg cagcagaagt tgctgcgatg tccaagcagt
    11641 cttaattatc gaaaaggagc cgcctgaggg cggcttcttt ttgtgcccat cttgtaaatt
    11701 cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca atgtcgctag tattaattca
    11761 tttcaatcgt tgacagtctc aggaaagaaa catggctacc aatgcaaaac ccgtctataa
    11821 acgcattctg cttaagttga gtggcgaagc tctgcagggc actgaaggct tcggtattga
    11881 tgcaagcata ctggatcgta tggctcagga aatcaaagaa ctggttgaac tgggtattca
    11941 ggttggtgtg gtgattggtg ggggtaacct gttccgtggc gctggtctgg cgaaagcggg
    12001 tatgaaccgc gttgtgggcg accacatggg gatgctggcg accgtaatga acggcctggc
    12061 aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt ctgatgtccg ctattccatt
    12121 gaatggcgtg tgcgacagct acagctgggc agaagctatc agcctgttgc gcaacaaccg
    12181 tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt accaccgact cagcagcttg
    12241 cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa gcaaccaaag ttgacggcgt
    12301 gtttaccgct gatccggcga aagatccaac cgcaaccatg tacgagcaac tgacttacag
    12361 cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg gccttcacgc tggctcgtga
    12421 ccataaatta ccgattcgtg ttttcaatat gaacaaaccg ggtgcgctgc gccgtgtggt
    12481 aatgggtgaa aaagaaggga ctttaatcac ggaataattc ccgtgatgga taaataaggg
    12541 taagattccg cgtaagtatc gcgggggcgt aagtctggtt ataaggcgtt attgttgcag
    12601 gcagtttggt cacggccagc gcgcagcaac cggagcgtac aaaagtacgt gaggatggcg
    12661 agcactgctc ggggccaaaa tggcaaataa aatagcctaa taatccagac gattacccgt
    12721 aatatgttta atcagggcta tacttagcac acttccactg tgtgtgactg tctggtctga
    12781 ctgagacaag ttttcaagga ttcgtaacgt gattagcgat atcagaaaag atgctgaagt
    12841 acgcatggac aaatgcgtag aagcgttcaa aacccaaatc agcaaaatac gcacgggtcg
    12901 tgcttctccc agcctgctgg atggcattgt cgtggaatat tacggcacgc cgacgccgct
    12961 gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact ctgaaaatca acgtgtttga
    13021 tcgttcaatg tctccggccg ttgaaaaagc gattatggcg tccgatcttg gcctgaaccc
    13081 gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg ctgacggaag aacgtcgtaa
    13141 agatctgacc aaaatcgttc gtggtgaagc agaacaagcg cgtgttgcag tacgtaacgt
    13201 gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa gataaagaga tcagcgaaga
    13261 cgacgatcgc cgttctcagg acgatgtaca gaaactgact gatgctgcaa tcaagaaaat
    13321 tgaagcggcg ctggcagaca aagaagcaga actgatgcag ttctgatttc ttgaacgaca
    13381 aaaacgccgc tcagtagatc cttgcggatc ggctggcggc gttttgcttt ttattctgtc
    13441 tcaactctgg atgtttcatg aagcaactca ccattctggg ctcgaccggc tcgattggtt
    13501 gcagcacgct ggacgtggtg cgccataatc ccgaacactt ccgcgtagtt gcgctggtgg
    13561 caggcaaaaa tgtcactcgc atggtagaac agtgcctgga attctctccc cgctatgccg
    13621 taatggacga tgaagcgagt gcgaaacttc ttaaaacgat gctacagcaa cagggtagcc
    13681 gcaccgaagt cttaagtggg caacaagccg cttgcgatat ggcagcgctt gaggatgttg
    13741 atcaggtgat ggcagccatt gttggcgctg ctgggctgtt acctacgctt gctgcgatcc
    13801 gcgcgggtaa aaccattttg ctggccaata aagaatcact ggttacctgc ggacgtctgt
    13861 ttatggacgc cgtaaagcag agcaaagcgc aattgttacc ggtcgatagc gaacataacg
    13921 ccatttttca gagtttaccg caacctatcc agcataatct gggatacgct gaccttgagc
    13981 aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg ccctttccgt gagacgccat
    14041 tgcgcgattt ggcaacaatg acgcccgatc aagcctgccg tcatccgaac tggtcgatgg
    14101 gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa aggtctggaa tacattgaag
    14161 cgcgttggct gtttaacgcc agcgccagcc agatggaagt gctgattcac ccgcagtcag
    14221 tgattcactc aatggtgcgc tatcaggacg gcagtgttct ggcgcagctg ggggaaccgg
    14281 atatgcgtac gccaattgcc cacaccatgg catggccgaa tcgcgtgaac tctggcgtga
    14341 agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc cgcaccggat tatgatcgtt
    14401 atccatgcct gaaactggcg atggaggcgt tcgaacaagg tcaggcagcg acgacagcat
    14461 tgaatgccgc aaacgaaatc accgttgctg cttttcttgc gcaacaaatc cgctttacgg
    14521 atatcgctgc gttgaattta tctgtactgg aaaaaatgga tatgcgcgaa ccacaatgtg
    14581 tggacgatgt gttatctgtt gatgcgaacg cgcgtgaagt cgccagaaaa gaggtgatgc
    14641 gtctcgcaag ctgaggataa tccggctaca gagagtcgcg ctatttgtta gcgtagggct
    14701 tcagtgatat agtctgcgcc atctgatcgt aagtagttgg ctttataagg tcaggtatgc
    14761 cgtggtttta cacggctttt ttttgtatag gcttcagtat tcctgagtac cgtaaaccct
    14821 gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa ccacttagcg aaaaattgcc
    14881 agcgcatggc tgccgtcatg ttgcgatcat tatggacggc aatggtcgct gggcaaaaaa
    14941 gcaagggagg attcgtgcct ttgggcataa agccggggca aaatccgtcc gccgggctgt
    15001 ctcttttgcg gccaacaacg gtattgaggc gttaacgctg tatgccttta gtagtgaaaa
    15061 ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa ctgtttgtgt gggcgctcga
    15121 tagcgaagta aaaagtctgc accgacataa cgtgcgtctg cgtattattg gcgataccag
    15181 tcgctttaac tcgcgtttgc aagaacgtat tcgtaaatct gaagcgctaa cagctgggaa
    15241 taccggtctg acgctgaata ttgcggcgaa ctacggtgga cgttgggata tagtccaggg
    15301 agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg caaccagatc agatagatga
    15361 agagatgcta aaccagcatg tctgtatgca tgaactggcc cctgtagatt tagtaattag
    15421 gactgggggg gagcatcgca ttagtaactt tttgctttgg caaattgcct atgccgaact
    15481 ttactttaca gatgttctct ggcccgattt cgatgaacaa gactttgaag gggcgttaaa
    15541 tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag cccggtgatg aaacagcctg
    15601 atggggggcg cttttgctga agtatcgcct gatatctgct tttgtgttaa tacccgtcgt
    15661 catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc atcgtaacgc tggtggtctg
    15721 catgctggca gcgtgggaat ggggacagct tagcggtttt accactcgtt cgcagcgagt
    15781 atggttggcg gtgttatgcg ggttattgtt ggcgctgatg ctttttctgt tgccggaata
    15841 tcaccgaaat attcatcaac cgctggttga aatctcactt tgggcttcgt tgggttggtg
    15901 gattgtcgcg ctattgctgg tgctgtttta cccaggttcc gcagcaatct ggcgtaactc
    15961 taaaacattg cgccttattt ttggcgtgct aaccattgtt cccttcttct ggggcatgct
    16021 ggcgttacgg gcctggcact atgacgagaa tcattacagt ggcgcaatat ggctgctcta
    16081 tgtcatgatc ctggtatggg gcgctgactc cggcgcatat atgtttggca aattgtttgg
    16141 taaacataag ctggcaccga aggtttctcc gggtaaaacc tggcaaggct ttataggtgg
    16201 actagcgacc gcagcggtaa tctcatgggg ttatggcatg tgggcgaatc tcgacgtcgc
    16261 tcccgtcacc ttactcattt gctctattgt cgcagcgtta gcctcagtgc tcggcgatct
    16321 gaccgagagt atgtttaagc gtgaagcagg aattaaggac agcggtcatt taattccagg
    16381 acacggtggt attttagatc gtattgatag cctgacggct gcggtaccgg tctttgcttg
    16441 cttgttgtta ctggtattca ggacgcttta acggaaggta atatgctgag ttttctctgg
    16501 gatttggctt cgttcatcgt tgcactgggt gtacttatca ccgtgcatga atttggtcat
    16561 ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt tctcaatagg gtttggtaag
    16621 gcgctctggc ggcgaactga taagctcggc accgaatatg ttatggccct gattccgctg
    16681 ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg tcgttccgga actccgccac
    16741 catgccttca ataataaatc tgttggccaa cgagcggcga ttattgccgc aggtccggtt
    16801 gcaaacttca tttttgctat ctttgcctac tggctggttt ttattattgg tgtgcctggc
    16861 gtacgtccgg tggttggtga aatagcagcc aattcgatag ctgcggaagc acaaattgca
    16921 ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc ctgattggga tgccgtgcgt
    16981 ttgcagttgg tcgataaaat tggcgatgaa agcaccacca ttacagtagc gccatttggc
    17041 agcgaccaac ggcgggatgt aaagctcgat ttacgtcact gggcgtttga gcctgataaa
    17101 gaagatccgg tatcttcgct ggggattcgt cctcgtgggc cgcaaattga acctgtactg
    17161 gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt tgcaagcagg cgacaggatc
    17221 gttaaagtcg atggtcagcc cttaacgcag tgggtgacct ttgtgatgct tgtccgggat
    17281 aacccgggta aatccttagc gttagaaatc gaaaggcagg ggagtccctt gtctttgaca
    17341 ttaatcccgg agagtaaacc gggtaatggt aaagcgattg gttttgtcgg tattgagccg
    17401 aaagtcattc ctttgccaga tgagtataaa gttgtacgcc agtatgggcc gttcaacgcc
    17461 atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc tgacggtcag tatgctggga
    17521 aaattgatca ccggtgatgt gaaactgaac aacctcagtg ggccgatctc tatcgccaag
    17581 ggggctggga tgacagcgga actcggggta gtttattacc tgccgtttct tgcgcttatt
    17641 agcgtgaact tagggataat taacctgttt ccgttgcccg tacttgacgg ggggcatctg
    17701 ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat ccgagcgggt tcaagacttt
    17761 tgttatcgca ttggctcgat tctgctggtg ctgttaatgg ggcttgcact tttcaatgat
    17821 ttctctcggt tatgagagtt agttaggaag aacgcataat aacgatggcg atgaaaaagt
    17881 tgctcatagc gtcgctgctg tttagcagcg ccaccgtata cggtgctgaa gggttcgtag
    17941 tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt tggtgcggca ctcctcagta
    18001 tgccggtgcg cacaggcgac acggttaatg atgaagatat cagtaatacc attcgcgctc
    18061 tgtttgctac cggcaacttt gaggatgttc gcgtccttcg tgatggtgat acccttctgg
    18121 ttcaggtaaa agaacgtccg accattgcca gcattacttt ctccggtaac aaatcggtga
    18181 aagatgacat gctgaagcaa aacctcgagg cttctggtgt gcgtgtgggc gaatccctcg
    18241 atcgcaccac cattgccgat atcgagaaag gtctggaaga cttctactac agcgtcggta
    18301 aatatagcgc cagcgtaaaa gctgtcgtga ccccgctgcc gcgcaaccgt gttgacctaa
    18361 aactggtgtt ccaggaaggt gtgtcagctg aaatccagca aattaacatt gttggtaacc
    18421 atgctttcac caccgacgaa ctgatctctc atttccaact gcgtgacgaa gtgccgtggt
    18481 ggaacgtggt aggcgatcgt aaataccaga aacagaaact ggcgggcgac cttgaaaccc
    18541 tgcgcagcta ctatctggat cgcggttatg cccgtttcaa catcgactct acccaggtca
    18601 gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa cattaccgaa ggcgatcagt
    18661 acaagctttc tggcgttgaa gtgagcggca accttgccgg gcactccgct gaaattgagc
    18721 agctgactaa gatcgagccg ggcgagctgt ataacggcac caaagtgacc aagatggaag
    18781 atgacatcaa aaagcttctc ggtcgctatg gttatgccta tccgcgcgta cagtcgatgc
    18841 ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt gaacgttgat gcgggtaacc
    18901 gtttctacgt gcgtaagatc cgttttgaag gtaacgatac ctcgaaagat gccgtcctgc
    18961 gtcgcgaaat gcgtcagatg gaaggtgcat ggctgggaag cgatctggtc gatcagggta
    19021 aggagcgtct gaatcgtctg ggcttctttg aaactgtcga taccgatacc caacgtgttc
    19081 cgggtagccc ggaccaggta gatgtagtct acaaggtgaa agaacgtaac accggtagct
    19141 tcaacttcgg gattggttac ggtactgaaa gtggcgtgag cttccaggct ggcgtgcaac
    19201 aggataactg gttaggtaca ggttatgctg ttggtatcaa cgggaccaaa aacgattacc
    19261 agacctatgc tgaattgtcg gtaaccaacc cgtacttcac tgtagatggt gtaagcctcg
    19321 gtggtcgtct cttctataat gacttccagg cagatgacgc cgacctgtcc gactatacca
    19381 acaagagtta tggtacagac gtgacgttgg gcttcccgat caacgaatat aactcgctgc
    19441 gtgcaggtct gggttatgta cataactccc tgtccaacat gcagcctcag gttgcgatgt
    19501 ggcgttatct gtactctatg ggtgaacatc cgagcacctc tgatcaggat aacagcttca
    19561 aaacggacga cttcacgttc aactatggtt ggacctataa caagcttgac cgtggttact
    19621 tcccgacaga tggttcacgt gtcaacctga ccggtaaagt gaccattcct ggatcggata
    19681 acgaatacta caaagtgacg ttagacacgg cgacttatgt gccgatcgat gacgatcaca
    19741 aatgggttgt tctggggcgt acccgctggg gttatggtga tggtttaggc ggcaaagaga
    19801 tgccgttcta cgagaacttc tatgccggtg gttccagcac cgtgcgtggc ttccagtcca
    19861 atacaattgg tccgaaagca gtttacttcc cgcatcaggc cagtaattat gatccggact
    19921 atgattacga atgtgcgact caggacggcg cgaaagacct gtgtaaatcg gatgatgctg
    19981 taggcggtaa cgccatggcg gttgccagcc tcgagttcat caccccgacg ccgtttatta
    20041 gcgataagta tgctaactcg gttcgtactt ccttcttctg ggatatgggt accgtttggg
    20101 atacaaactg ggattccagc caatattctg gatatccgga ctatagtgat ccaagcaata
    20161 tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc attggggccg ttggtgttct
    20221 cctacgccca gccgttcaaa aagtacgatg gagacaaggc agaacagttc cagtttaaca
    20281 tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag tggtagtgta gcgatgactt
    20341 taggcgatca atataagatc gccgggccac gcaaagaact gcaccctccg gtgcaaatgg
    20401 gatggtaagg agtttattgt gaaaaagtgg ttattagcgg caggtctcgg tttagcactg
    20461 gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca atatgggcag cctgttccag
    20521 caggtagcgc agaaaaccgg tgtttctaac acgctggaaa atgagttcaa aggccgtgcc
    20581 agcgaactgc agcgtatgga aaccgatctg caggctaaaa tgaaaaagct gcagtccatg
    20641 aaagcgggca gcgatcgcac taagctggaa aaagacgtga tggctcagcg ccagactttt
    20701 gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc gttccaacga agaacgcggc
    20761 aaactggtta ctcgtatcca gactgctgtg aaatccgttg ccaacagcca ggatatcgat
    20821 ctggttgttg atgcaaacgc cgttgcttac aacagcagcg atgtaaaaga catcactgcc
    20881 gacgtactga aacaggttaa ataagtaatg ccttcaattc gactggctga tttagcgcag
    20941 cagttggatg cagaactaca cggtgatggc gatatcgtca tcaccggcgt tgcgtccatg
    21001 caatctgcac aaacaggtca cattacgttc atggttaacc caaaataccg tgagcattta
    21061 ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg atcttccttt cgcgaaaagt
    21121 gccgcgctgg tagtgaagaa tccctacctg acttacgcgc gcatggcgca aattttagat
    21181 accacgccgc agcccgcgca gaacattgca cccagtgcgg tgatcgacgc gacggcgaag
    21241 ctgggtaaca acgtatcgat tggcgctaac gcggtgattg agtccggcgt tgaactgggc
    21301 gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa acagcaaaat cggtgcaggt
    21361 tcgcgtctct gggcgaacgt aaccatttac catgagatcc agatcggtca gaattgcctg
    21421 atccagtccg gaacagtggt aggcgcagac ggctttggtt atgccaacga tcgtggtaac
    21481 tgggtgaaga tcccacagat tggtcgcgta attattggcg atcgcgtgga gatcggtgcc
    21541 tgcacaacca tcgatcgcgg cgcgctggat gacactgtta ttggcaatgg cgtgatcatt
    21601 gataaccagt gccagattgc acataacgtc gtgattggcg acaatacggc ggttgccggt
    21661 ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact gcatgatcgg cggagccagc
    21721 gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg ttacgggcat gggtatggtg
    21781 atgcgtccca tcactgaacc aggcgtctat tcctcaggca ttccgctgca acccaacaaa
    21841 gtctggcgca aaaccgctgc actggtgatg aacattgatg acatgagcaa gcgtctgaaa
    21901 tcgcttgagc gcaaggttaa tcaacaagac taacgttcca tcttttgttc gccaaacttt
    21961 acggcctgtc tcattcttac gattgcggca ggccgtgtta ttattgtcgt ttcttatatt
    22021 ttgacaggaa gagtatcttg actactaaca ctcatactct gcagattgaa gagattttag
    22081 aacttctgcc gcaccgtttc ccgttcttac tggtggatcg cgtgctggat tttgaagaag
    22141 gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga gccattcttc cagggccatt
    22201 tccctggaaa accgattttc ccgggtgtgc tgattctgga agcaatggca caggcaacag
    22261 gtattctggc gtttaaaagc gtaggaaaac tggaaccggg tgagctgtac tacttcgctg
    22321 gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg cgatcaaatg atcatggaag
    22381 tcactttcga aaaaacgcgc cgcggcctga cccgttttaa aggggttgct ctggtcgatg
    22441 gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag ccgggaggcc tgatacgtga
    22501 ttgataaatc cgcctttgtg catccaaccg ccattgtgga agagggcgcg tcaattggcg
    22561 cgaacgcaca cattggtcct ttttgtatcg ttggacccca tgtcgaaatt ggtgagggta
    22621 ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa aattggccgc gataatgaga
    22681 tttatcagtt cgcctccatc ggcgaagtta accaggatct gaaatatgct ggcgaaccga
    22741 cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag cgtcaccatt catcgtggca
    22801 cagtccaggg cggtggattg acgaaggtgg gcagcgacaa cttactgatg atcaacgcgc
    22861 acattgcgca cgattgtacg gtaggtaacc gctgtattct cgccaacaac gcaacgctgg
    22921 cgggtcacgt atcggttgac gacttcgcga tcatcggcgg catgaccgca gtccatcagt
    22981 tctgcatcat tggtgcgcac gtgatggttg gcggctgctc cggtgtggcg caggacgtcc
    23041 ctccttatgt cattgcgcag ggtaaccacg caacgccgtt cggtgtcaat atcgaagggc
    23101 tgaagcgccg cggattcagc cgtgaggcga ttaccgctat ccgcaatgcg tataagctga
    23161 tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga aattgctgaa ctggcggaaa
    23221 catatccgga agtgaaagcc tttaccgatt tctttgcacg ctcaacgcgc ggtctgattc
    23281 gttaatgact gaacagcgtc cattaacgat tgccctggtc gccggagaaa cctccggcga
    23341 tatcctgggg gccggtttaa tccgcgctct gaaagaacat gtgcccaacg cccgctttgt
    23401 tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa gcttggtacg aaatggaaga
    23461 actggcggta atgggcattg ttgaagtgct cggtcgtctg cgtcgcttac tgcatattcg
    23521 tgccgatctg acaaagcgtt ttggcgaact gaagccagat gtttttgttg gtattgatgc
    23581 gcccgacttc aatattactc ttgaaggtaa cctcaaaaag cagggtatca aaaccattca
    23641 ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc gttttcaaaa taggcagagc
    23701 caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg ttttatgaca aatacaatgt
    23761 accgtgccgc tttatcggtc ataccatggc tgatgccatg ccgttagatc cagataaaaa
    23821 tgccgcccgt gatgtgctgg ggatccctca cgatgcccac tgcctggcgt tgctaccggg
    23881 gagccgtggt gcagaagttg aaatgcttag tgccgatttc ctgaaaacgg cccagctttt
    23941 gcgccagaca tatccggatc tcgaaatcgt ggtgccactg gtgaatgcca aacgccgcga
    24001 gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctt tcagttcatt tgctggatgg
    24061 gatgggccgt gaggcgatgg tcgccagcga tgcggcgcta ctggcgtcgg gtacggcagc
    24121 cctggagtgt atgctggcga aatgcccgat ggtggtggga tatcgcatga agccttttac
    24181 cttctggttg gcgaagcggc tggtgaaaac tgattatgtc tcgctgccaa atctgctggc
    24241 gggcagagag ttagttaaag aattattgca ggaagagtgt gagccgcaaa aactggctgc
    24301 ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac gcgatgcacg ataccttccg
    24361 tgaactgcat cagcagatcc gctgcaatgc cgatgagcag gcggcacaag ccgttctgga
    24421 gttagcacaa tgatcgaatt tgtttatccg cacacgcagc tggttgcggg tgtggatgaa
    24481 gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg cggtgatcct tgacccggcg
    24541 cgcccgattg ccgggctgaa tgattccaaa aagctgagcg aaaaacgccg tctggcgctc
    24601 tatgaagaga tcaaagagaa agcgttgagc tggagtctgg gccgcgcgga accccacgaa
    24661 atcgacgagc tgaacattct tcatgcgacc atgctggcga tgcagcgtgc cgtcgctggg
    24721 ctgcatattg cgccggaata tgtgttgatt gatggtaacc gctgcccgaa attaccgatg
    24781 cctgcgatgg ctgtggtgaa aggcgatagc cgcgtaccgg aaatcagtgc cgcgtctatc
    24841 ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg atattgtttt cccgcaatat
    24901 ggttttgccc aacacaaagg gtacccaacc gcttttcatc tggaaaaact ggctgaacac
    24961 ggcgcgaccg aacaccatcg gcgcagcttt gggcctgtca aacgcgcact gggacttgcg
    25021 tcctgattct tgtgtcgaga ttaagtaaac cggaatctga agatgtctga accacgtttc
    25081 gtacacctgc gggtgcacag cgactactcg atgatcgatg gcctggccaa aaccgcaccg
    25141 ttggtaaaaa aggcggcggc gttgggtatg ccagcactgg cgatcaccga tttcaccaac
    25201 ctttgtggtc tggtgaagtt ctacggagcg ggacatggcg cagggattaa gcctatcgtc
    25261 ggggcagatt ttaacgtcca gtgcgacctg ctgggtgatg agttaaccca cctgacggta
    25321 ctggcggcga acaataccgg ctatcagaat ctgacgttgc tgatctcaaa agcgtatcag
    25381 cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact ggcttatcga attaaacgaa
    25441 gggttgatcc ttctttccgg cggacgcatg ggcgacgtcg gacgcagtct tttgcgtggt
    25501 aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag aacacttccc ggatcgctat
    25561 tttcttgagc tgatccgcac cggcaggccg gacgaagaaa gctatctgca cgcggcggtg
    25621 gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacca acgacgtgcg ctttatcgac
    25681 agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc acgacggctt taccctcgac
    25741 gatcctaaac gcccgcgtaa ctattcgccg cagcaatata tgcgtagcga agaggagatg
    25801 tgcgagctgt ttgccgacat ccccgaagct cttgccaaca ccgttgagat cgccaaacgt
    25861 tgtaacgtaa ccgtgcgtct tggtgaatac ttcctgccgc agttcccgac cggggacatg
    25921 agcaccgaag attatctggt caagcgtgca aaagagggcc tggaagagcg tctggccttt
    25981 ttattccctg acgaggaaga acgtgttaag cgccgcccgg aatatgacga acgtctggag
    26041 actgaacttc aggttatcaa ccagatgggc ttcccgggct acttcctcat cgttatggaa
    26101 tttatccagt ggtcgaaaga taacggcgta ccggtagggc caggccgtgg ctccggtgcg
    26161 ggttcactgg tggcctacgc gctgaaaatc accgacctcg atccgctgga atttgacctg
    26221 ctgttcgaac gtttccttaa cccggaacgt gtctccatgc ctgacttcga cgttgacttc
    26281 tgtatggaga aacgcgatca ggttatcgag cacgtagcgg acatgtacgg tcgtgatgcg
    26341 gtatcgcaga tcatcacctt cggtacaatg gcggcgaaag cggtgatccg cgacgtaggc
    26401 cgcgtgctgg ggcatccgta cggctttgtc gatcgtatct cgaaactgat cccgcccgat
    26461 ccggggatga cgctggcgaa agcgtttgaa gccgagccgc agctgccgga aatctacgaa
    26521 gcggatgaag aagttaaggc gctgatcgac atggcgcgca aactggaagg ggtcacccgt
    26581 aacgccggta agcacgccgg tggggtggtt atcgcgccga ccaaaattac cgattttgcg
    26641 ccgctttact gcgatgaaga gggcaaacat ccggtcaccc agtttgataa aagcgacgtt
    26701 gaatacgccg gactggtgaa gttcgacttc cttggtttgc gtacgctcac catcatcaac
    26761 tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg gcgagccgcc gctggatatc
    26821 gctgcgatcc cgctggatga taagaaaagc ttcgacatgc tgcaacgctc ggaaaccacg
    26881 gcggtattcc agcttgaatc gcgcggcatg aaggacctga tcaagcgtct gcaacctgac
    26941 tgcttcgaag atatgatcgc actggtggca ctgttccgcc ccggcccgtt gcagtcaggg
    27001 atggtggata actttatcga ccgtaaacat ggtcgcgaag agatttccta tccggacgta
    27061 cagtggcagc atgaaagcct gaaaccggta ctggagccaa cctacggcat catcctgtat
    27121 caggaacagg tcatgcagat tgcgcaggtg ctttctggtt ataccctcgg tggcgcggat
    27181 atgctgcgtc gtgcgatggg taagaaaaag ccggaagaga tggctaagca gcgttctgta
    27241 tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac tggcgatgaa aatcttcgac
    27301 ctggtggaga aattcgcggg ttacggattt aacaaatcgc actctgcggc ctatgctttg
    27361 gtgtcatatc aaacgttatg gctgaaagcg cactatccgg cggagtttat ggcggcggta
    27421 atgaccgccg atatggacaa caccgagaag gtggtgggcc tggtggatga gtgctggcgg
    27481 atggggctga aaatcctgcc accagatata aactccggtc tttaccattt ccacgtcaac
    27541 gacgacggcg aaatcgtgta tggtattggc gccatcaaag gggtaggtga aggtccgatt
    27601 gaggccatca tcgaagcccg taataaaggc ggctacttcc gcgaactgtt tgatctctgc
    27661 gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg aaaaactgat catgtccggg
    27721 gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga actcgctggg tgatgcgtta
    27781 aaagcggcag atcaacacgc gaaagcggaa gctatcggtc aggccgatat gttcggcgtg
    27841 ctggccgaag agccggaaca aattgaacaa tcctacgcca gctgccaacc gtggccggag
    27901 caggtggtgt tagatgggga acgtgaaacg ttaggtctgt accttacggg acaccctatc
    27961 aaccagtatt taaaagagat tgagcgttat gtcggaggcg taaggctgaa agacatgcac
    28021 ccgacagaac gtggtaaagt catcacggct gcggggctcg ttgttgccgc gcgggttatg
    28081 gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg atgaccgttc cgggcggctg
    28141 gaagtgatgt tgtttactga cgccctggat aaataccagc aattgctgga aaaagaccgc
    28201 atacttatcg tcagcggaca ggtcagcttt gatgacttca gcggtgggct taaaatgacc
    28261 gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat atgctcgcgg gcttgctatc
    28321 tcgctgacgg acaggcaaat tgatgaccag cttttaaacc gactccgtca gtctctggaa
    28381 gcccaccgct ctgggacaat tccagtacat ctctactatc agagggcgga tgcacgcgcg
    28441 cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg atcgtttatt aaacgatctc
    28501 cgtggcctca ttggttcgga gcaggtggaa ctggagtttg actaatacag gaatactatg
    28561 agtctgaatt tccttgattt tgaacagccg attgcagagc tggaagcgaa aatcgattct
    28621 ctgactgcgg ttagccgtca ggatgagaaa ctggatatta acatcgatga agaagtgcat
    28681 cgtctgcgtg aaaaaagcgt agaactgaca cgtaaaatct tcgccgatct cggtgcatgg
    28741 cagattgcgc aactggcacg ccatccacag cgtccttata ccctggatta cgttcgcctg
    28801 gcatttgatg aatttgacga actggctggc gaccgcgcgt atgcagacga taaagctatc
    28861 gtcggtggta tcgcccgtct tgacggtcgt ccggtgatga tcattggtca tcaaaaaggt
    28921 cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc cagcgccaga aggttaccgc
    28981 aaagcactgc gtctgatgca aatggctgaa cgctttaaga tgcctatcat cacctttatc
    29041 gacaccccgg gggcttatcc tggcgtgggc gcagaagagc gtggtcagtc tgaagccatt
    29101 gcacgcaacc tgcgtgaaat gtctcgcctc ggcgtaccgg tagtttgtac ggttatcggt
    29161 gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg ataaagtgaa tatgctgcaa
    29221 tacagcacct attccgttat ctcgccggaa ggttgtgcgt ccattctgtg gaagagcgcc
    29281 gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg ctccgcgtct gaaagagctg
    29341 aaactgatcg actccatcat cccggaaccg ctgggcggcg ctcaccgtaa cccggaagcg
    29401 atggcggtat cgttgaaagc gcaactgctg gcggatctgg ccgatctcga tgtgttaagc
    29461 actgaagatt taaaaaatcg tcgttatcag cgcctgatga gctacggtta cgcgtaattc
    29521 gcaaaagttc tgaaaaaggg tcacttcgg
//
LOCUS       DAZOZY010000029        29368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZOZY010000029 DAZOZY010000000
VERSION     DAZOZY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.029"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            43..1482
                     /gene="norV"
                     /locus_tag="ACHURQ_001146"
     CDS             43..1482
                     /gene="norV"
                     /locus_tag="ACHURQ_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR0822981.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1479..2612
                     /gene="norW"
                     /locus_tag="ACHURQ_001147"
     CDS             1479..2612
                     /gene="norW"
                     /locus_tag="ACHURQ_001147"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR0822982.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2740..4992)
                     /gene="hypF"
                     /locus_tag="ACHURQ_001148"
     CDS             complement(2740..4992)
                     /gene="hypF"
                     /locus_tag="ACHURQ_001148"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR0822983.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTISQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5145..5672)
                     /gene="hydN"
                     /locus_tag="ACHURQ_001149"
     CDS             complement(5145..5672)
                     /gene="hydN"
                     /locus_tag="ACHURQ_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR0822984.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5820..6830)
                     /gene="ascG"
                     /locus_tag="ACHURQ_001150"
     CDS             complement(5820..6830)
                     /gene="ascG"
                     /locus_tag="ACHURQ_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR0822985.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7090..8547
                     /gene="ascF"
                     /locus_tag="ACHURQ_001151"
     CDS             7090..8547
                     /gene="ascF"
                     /locus_tag="ACHURQ_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR0822986.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            8556..9980
                     /gene="ascB"
                     /locus_tag="ACHURQ_001152"
     CDS             8556..9980
                     /gene="ascB"
                     /locus_tag="ACHURQ_001152"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR0822987.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10094..10513)
                     /locus_tag="ACHURQ_001153"
     CDS             complement(10094..10513)
                     /locus_tag="ACHURQ_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0822988.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(10510..10821)
                     /locus_tag="ACHURQ_001154"
     CDS             complement(10510..10821)
                     /locus_tag="ACHURQ_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR0822989.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(10983..11756)
                     /locus_tag="ACHURQ_001155"
     CDS             complement(10983..11756)
                     /locus_tag="ACHURQ_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0822990.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(11781..12251)
                     /gene="hycI"
                     /locus_tag="ACHURQ_001156"
     CDS             complement(11781..12251)
                     /gene="hycI"
                     /locus_tag="ACHURQ_001156"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR0822991.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(12244..12654)
                     /gene="hycH"
                     /locus_tag="ACHURQ_001157"
     CDS             complement(12244..12654)
                     /gene="hycH"
                     /locus_tag="ACHURQ_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR0822992.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(12651..13418)
                     /gene="hycG"
                     /locus_tag="ACHURQ_001158"
     CDS             complement(12651..13418)
                     /gene="hycG"
                     /locus_tag="ACHURQ_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR0822993.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(13418..13960)
                     /gene="hycF"
                     /locus_tag="ACHURQ_001159"
     CDS             complement(13418..13960)
                     /gene="hycF"
                     /locus_tag="ACHURQ_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR0822994.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(13970..15667)
                     /gene="hycE"
                     /locus_tag="ACHURQ_001160"
     CDS             complement(13970..15667)
                     /gene="hycE"
                     /locus_tag="ACHURQ_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR0822995.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(15685..16608)
                     /gene="hycD"
                     /locus_tag="ACHURQ_001161"
     CDS             complement(15685..16608)
                     /gene="hycD"
                     /locus_tag="ACHURQ_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR0822996.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(16611..18437)
                     /gene="hycC"
                     /locus_tag="ACHURQ_001162"
     CDS             complement(16611..18437)
                     /gene="hycC"
                     /locus_tag="ACHURQ_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR0822997.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(18434..19045)
                     /gene="hycB"
                     /locus_tag="ACHURQ_001163"
     CDS             complement(18434..19045)
                     /gene="hycB"
                     /locus_tag="ACHURQ_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR0822998.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(19170..19631)
                     /gene="hycA"
                     /locus_tag="ACHURQ_001164"
     CDS             complement(19170..19631)
                     /gene="hycA"
                     /locus_tag="ACHURQ_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR0822999.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            19843..20193
                     /gene="hypA"
                     /locus_tag="ACHURQ_001165"
     CDS             19843..20193
                     /gene="hypA"
                     /locus_tag="ACHURQ_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0823000.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            20197..21069
                     /gene="hypB"
                     /locus_tag="ACHURQ_001166"
     CDS             20197..21069
                     /gene="hypB"
                     /locus_tag="ACHURQ_001166"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR0823001.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            21060..21332
                     /gene="hypC"
                     /locus_tag="ACHURQ_001167"
     CDS             21060..21332
                     /gene="hypC"
                     /locus_tag="ACHURQ_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR0823002.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            21332..22453
                     /gene="hypD"
                     /locus_tag="ACHURQ_001168"
     CDS             21332..22453
                     /gene="hypD"
                     /locus_tag="ACHURQ_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR0823003.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            22450..23460
                     /gene="hypE"
                     /locus_tag="ACHURQ_001169"
     CDS             22450..23460
                     /gene="hypE"
                     /locus_tag="ACHURQ_001169"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR0823004.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            23534..25611
                     /gene="flhA"
                     /locus_tag="ACHURQ_001170"
                     /pseudo
     CDS             23534..25611
                     /gene="flhA"
                     /locus_tag="ACHURQ_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(25648..26001)
                     /locus_tag="ACHURQ_001171"
     CDS             complement(25648..26001)
                     /locus_tag="ACHURQ_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR0823005.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(26078..26212)
                     /locus_tag="ACHURQ_001172"
     CDS             complement(26078..26212)
                     /locus_tag="ACHURQ_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823006.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            26288..28849
                     /gene="mutS"
                     /locus_tag="ACHURQ_001173"
     CDS             26288..28849
                     /gene="mutS"
                     /locus_tag="ACHURQ_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR0823007.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            28955..29368
                     /locus_tag="ACHURQ_001174"
                     /pseudo
     CDS             28955..29368
                     /locus_tag="ACHURQ_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT         6989 a         8038 c         7808 g         6533 t
ORIGIN      
        1 caagacatct ttttagaaca cgctgaataa attgaggttg ctatgtctat tgtggtgaaa
       61 aataacattc attgggttgg tcaacgtgac tgggaagtgc gtgattttca cggtacggaa
      121 tataaaacgc tacgcggcag cagttacaac agctacctca tccgcgaaga aaaaaacgtg
      181 ctgatcgaca ccgtcgacca taaattcagc cgcgaatttg tgcagaacct gcgtaatgaa
      241 atcgatctgg cggatatcga ttacatcgtg attaaccatg cagaagagga ccacgctggg
      301 gcgctgaccg aactgatggc gcaaattccc gatacgccga tttactgtac tgccaacgct
      361 atcgactcga taaatggtca tcatcatcat ccggagtgga attttaatgt ggtgaaaact
      421 ggtgacacgc tggatatcgg caacggcaaa cagctcattt ttgtcgaaac gccaatgctg
      481 cactggccgg acagcatgat gacttacctg acaggcgacg cggtgctgtt cagtaacgat
      541 gctttcggtc agcactactg cgacgagcat ctgttcaacg atgaagtgga tcagacggag
      601 cttttcgagc agtgccagcg ttactacgcc aatatcctga cgccgttcag ccgcctggta
      661 acaccgaaaa ttaccgagat cctgggcttt aacttaccag tcgatatgat agcgacctct
      721 cacggcgtgg tatggcgcga taacccgacg caaattgtcg agctgtacct gaaatgggcg
      781 gctgattatc aggaagacag aatcaccatt ttctacgaca ccatgtcgaa taacacccgc
      841 atgatggctg acgctatcgc ccaggggatt gcggaaaccg acccacgcgt ggcggtgaaa
      901 attttcaacg tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt ccgctcaaaa
      961 ggcgtgctgg tcggcacttc gacgatgaat aacgtgatga tgccgaaaat cgccggtctg
     1021 gtggaggaga tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt cggctctcac
     1081 ggctggagcg gcggtgcggt ggatcgtctt tccacgcgcc tgcaggatgc gggtttcgaa
     1141 atgtcgctta gcctgaaagc caaatggcga ccagaccagg acgctctgga gttatgccgt
     1201 gaacacggtc gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca gagcacggtg
     1261 aatacggtgg ttaaagaaga aacctctgcc accacgaagt ccgacctcgg cccacggatg
     1321 cagtgcagcg tctgccagtg gatttacgat ccggcaaaag gcgagccaat gcaggacgtt
     1381 gcgccaggaa cgccgtggag tgaagttccg gataacttcc tctgcccgga atgctctctc
     1441 ggcaaagacg tctttgacga actggcatcg gaggcaaaat gagcaacggc attgtgatca
     1501 ttggttcggg cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag gacgccagta
     1561 ttccattaac cctgatcgcc gccgacagca tggatgagta caacaaaccg gacctcagcc
     1621 atgttatcag ccaggggcaa cgtgccgacg accttacccg ccagacggcg ggtgaatttg
     1681 ccgggcagtt taatctgcac ctgtttccgc acacctgggt gacggatatc gatgccgaag
     1741 cccatgtggt gaaaagccag aataatcagt ggcaatacga caagctggtg ctggcaactg
     1801 gtgccagtgc ctttgtcccg ccagtgcctg ggcgtgagtt aatgctgacg ttaaatagtc
     1861 agcaagagta tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg ttgattgtcg
     1921 gcggtggttt gattggtagc gaactggcga tggatttttg tcgtgcaggc aaagcggtca
     1981 cgctaatcga caacgctgcc agtattctgg cgtcattaat gccaccagaa gtaagcagcc
     2041 gcttgcagca tcggttgacg gagatgggcg ttcatctgct gttgaaatct cagttgcagg
     2101 ggctggaaaa aacggattct ggcattctgg caacgctgga ccaccagcgt agtatcgaag
     2161 tggatgcggt aattgccgcc accggactgc gcccggaaac agccctggca cgacgcgccg
     2221 ggctgacgat taatcgcggc gtttgcgtcg atagttatct gcaaaccagt aatgacgata
     2281 tttatgcgct gggcgattgc gcggaaatca acggtcaggt actgccattc ctgcagccga
     2341 ttcaacttag cgcgatggtg ctggcaaaaa atcttctcgg caataacacg ccgctgaaac
     2401 taccggcgat gctggtgaaa atcaaaacgc cggaattacc gctgcatctg gcaggcgaaa
     2461 cccagcgtca ggatttacgc tggcaaatta ataccgaacg ccagggaatg gtggctcgcg
     2521 gtgttgacga tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg atgaaagagg
     2581 catttggatt gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa aatgtcggat
     2641 gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccgg ataaggcgtt
     2701 tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct ggacttcacc
     2761 cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc catcacccgc
     2821 cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca gacgtgcacg
     2881 cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac cacgcatcgt
     2941 ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa acgcccacgc
     3001 gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagcgg cgagatccag
     3061 ttgattgtcc accagcggca tcgtcaccgg atgcgtcact ccgtggcatg aggctgcgag
     3121 cgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg cacagcccag
     3181 tgccgccgcc actgcatcga acaaccgccc acacgatgac gccagcggcg cgttaattcc
     3241 acgctcaatg gcccgcgcca gcacgctcca gttttgctgt tgcacacttg ctgtttcagg
     3301 gtaattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc gccacggctg
     3361 cttcgctgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca ggtgctcaca
     3421 ttcgcgatag ttcacccgca ggcactcgcc gccccacaaa gcgccgttct cccccatacc
     3481 gataccgtcg agcgtcaaag caatgacatc accgccatcc aacggccact gatgctctgc
     3541 cagacacgcc gctgcatggg catgatgatg cagtaccgtt tgcgtcggca gattcatttc
     3601 acgcgcccac tggctggaga catagcccgg atgcgcgtca tgcacaacgt attgcggggt
     3661 aaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct ggatgccatc
     3721 gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcaccgcgca ccaggcagaa
     3781 ggtatttttc agatccgcgc cgagacacag cacaggcgga acatttttaa agcccggagg
     3841 caaagccagc gcatccggca catacccccg cgaacggcgc agcatttcgc cgctttcgcg
     3901 caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga atccgtcggc
     3961 aatgccctgc aaatccgcca gcgcctgttc gttgctgata gctggtggtt taccgctcag
     4021 gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat gctggagcgg
     4081 gttcgcaggc aacattaccc cgacttcgtt aaggtcaggg gcgatatcat cacaaagctc
     4141 aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa gcaactggcg
     4201 cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt tcgccgggcg
     4261 atgtttgcgc gcccgaagtg tcgccaccgc gttactgtta cgtgcatcgc aggcaagatg
     4321 aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg cgatagccgc
     4381 ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa gatgtgggcc
     4441 acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt cacggtactc
     4501 tttgtcacaa gccggacaca acggaaacgc cgccatcacg gtaaacgggc ggtcgtaagg
     4561 catggcgcga ataatggtga aacgcgggcc gcagtgggta cagttgataa acggataacg
     4621 ataacgccgt tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag tagcggcatc
     4681 cgggacaatt tgcgtattca tggcacctcc cgcgctctgg ctgatggtga actcagtggg
     4741 cagttgtgac cagatgtacg gctcacgctc gacgctatca atacgcgcca gcggcgggca
     4801 gtgctgatgc aattgaacaa gtaacgtttc cgggtcttcc cgcagccgga cttctacgcc
     4861 atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct gccagacaaa
     4921 cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac cgcaagatgt
     4981 gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgtcc tgccggacac
     5041 gactgcgtcg tatccggcag tctcaggtcg gcgataccgc cagctccgta ttctacgaat
     5101 atttccggga attcctttga tgccagaaca gttctgtaag atttttagaa catcagcgcc
     5161 gtacggcggc gtttttctgc actcagttgt tcaagtttat tacgatcgac acaaatcagc
     5221 gcatgagtcg ggcaagccgc catacacgcc gggccgtctt cacgatggtt gcacaggtca
     5281 catttattgg cttcggcttt gtcagcccgt acattcagcc ccgcgccgct gttgcgaatc
     5341 accggacgta ccaccacttc catcgcgcca tacgggcaag ccacaacgca ggttttgcaa
     5401 ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat agcaccattc
     5461 gggcagacgt tagcgcacgg tgcatcttca cactgacggc aaactgtcgc cgtggaaatg
     5521 ttcacacctt taatgacatg gatacgcggt aaaaatgttt ccggggtcag cgatgcacag
     5581 tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacggca accaatacat
     5641 ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga cagttaatgc
     5701 gccgatacat tcacaaatca tgccagtttt aattcactgt tatttaagga aattaatttc
     5761 tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga tgacatgtga tgacaatgtt
     5821 tatcgcgaaa gagcaatgag tgagtcgcgg cggatcagtt ttccgctgaa ggttttcggc
     5881 ggtgagaaat ccccgccatc gagcataaaa atcagccgtc caataatttc ctgaatcatc
     5941 tcagtcaccg gaatttttac gctggagagc gccggaacga tatagggggc aattgcgata
     6001 tcatcgaatc cgataactga cacctgctct ggcaccgcta cgccgcgctc gtgcaacgct
     6061 ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta acgcgctaaa tttagcccca
     6121 cgttcgagca acgtttctac cccttcggcc ccgctggcgg gcgtccattt accgttagcg
     6181 ataagttttt cattgagcgc aataccatgc tgcgccagcg cgtctttata cccagcaaga
     6241 cgttcaatgc tggtggggga atccatcgag ccggtaagga aagcaatctc ctgatgtccg
     6301 gcgtttatca actctgccac ggcattaaag ctggtctgtt tatgatcgca ccagacgcta
     6361 tggctgctgt ttttgcgcag gcggcgatta agcaccatta tcggctgact gtgcgcgtca
     6421 atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt aaatcataat ggcgtcgcag
     6481 cgcaaatcca gcagatactg aatcgcctgg cgctcttctt cggcgctgtg tttaccatct
     6541 gccaatagca actgccgccc tttctcttcc gccattcgcg cggcatgaaa gagtaattca
     6601 ctaaaataaa tgccgtggta aagcgtgttg gtcactacca accccagcgt ctgagtactc
     6661 ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac cgctctcttc taccgcctga
     6721 aacacgcgat ctttagtctc ctggctgacg tagccattac ctgaaagcac gcgggaaacg
     6781 gtcgcttttg aaaccccggc gcgcttcgcc acttccagca tcgtcgtcat cattttcatc
     6841 cctttacacg caatcaacgc agtgtactgc accgtttgcc gattgtcctt gcgcaatcgg
     6901 cgggaaaaaa attcaggtga ccggtttcac aaatataaaa aatgaacaat taactctctt
     6961 gcttatttag tgacaactat tcatgatttt gtgaaaccgg tttcctaatt ccgtttcagc
     7021 atcggcattt ttccgttacg tcgactgata acaactacat ctaccctact gataacagga
     7081 taaaatccga tggccaaaaa ttatgcggcg ctggcacgct cggtgatagc ggcactgggc
     7141 ggcgttgata acatctcggc ggtcacgcac tgtatgacgc gtttgcgcgt tgttatcaaa
     7201 gatgaccagc ttatcgacag cccgacgtta aaaaccatcc ccggcgtgct cggcgtggta
     7261 cgtagtgaca accagtgtca ggtgattatc ggcaataccg tttcacaagc ctttcaggaa
     7321 gtcgtcagcc tgctgccggg agatatgcag cccgcacagc ccgtgggtaa acccaaactc
     7381 acgctgcgtc gcattggtgc ggggatcctc gatgcgctga tcggcaccat gtcaccgctg
     7441 atcccggcga ttatcggcgg atcgatggtc aaactgctgg cgatgatcct cgagatgagc
     7501 ggcgtgctga caaaaggatc gccgacctta accattctga atgtgattgg tgacggtgct
     7561 ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca tcaaatttaa aaccaatatg
     7621 tcgctggcga ttgccatcgc gggtgtgctg gtacatccga gttttattga actgatggcg
     7681 aaagcggccc agggtgaaca tgtcgaattt gccctgattc cggtcaccgc ggtgaaatac
     7741 acctacacag tgatcccggc gctggtgatg acctggtgcc tgtcatatat cgaacgctgg
     7801 gtggatcgca ttacaccggc ggtgaccaaa aacttcctca agccaatgct gattgtgttg
     7861 attgccgcac cgctggcaat cctgctgatt ggcccgattg gtatctggat cggtagcgcc
     7921 atttcggcgc tggtttacac cattcatggt tatctgggct ggctttcagt cgccattatg
     7981 ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc gcgtctttac gccaaccatc
     8041 attcagacca ttgccgaaac cggcaaagaa gggatggtca tgccgtcaga gatcggcgct
     8101 aacctgtcgc tgggcggttc atcactggcg gtggcgtgga aaacgaaaaa cccggaactg
     8161 cgccagacgg cgctggctgc ggcggcatca gccattatgg cggggatttc cgaaccggcg
     8221 ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg ccagtcttat cagcggtttt
     8281 atttgcggcg cggttgccgg tatggcgggg cttgccagcc actcaatggc agcgccaggg
     8341 ttatttacca gcgtgcaatt cttcgatccg gcgaatccaa tgagcatcgt ctgggtgttc
     8401 gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca cactgttgct cggctttgag
     8461 gatattcctg ttgaggaagc ggctgccgag gcgcgaaagc atcagagcgt acaaccgacc
     8521 gtcgccaaag aagtaagtct taattgagga tgaaaatgtc agtatttcca gaaggttttt
     8581 tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc gttccgtgaa ggtggcaaag
     8641 ggctgaccac tgtcgatatg atcccacacg gcgagcatcg aatggcggtg aaactggggc
     8701 tggaaaaacg ttttcagttg cgcgatgacg agttttatcc cagccatgag gcgacggatt
     8761 tttatcatcg ttataaagaa gatatcgccc tgatggcaga gatgggattc aaggttttcc
     8821 gtacctcaat tgcctggagc cgtctctttc cgcagggcga tgaaatcacg cccaatcagc
     8881 agggcattgc tttttatcgt tctgtctttg aagagtgtaa aaagtacggt atcgaaccgc
     8941 tggtcacgtt gtgccacttc gatgtgccga tgcatctggt caccgaatat ggctcctggc
     9001 gtaaccgcaa gctggtggag tttttcagcc gctacgcccg gacctgcttt gaagcatttg
     9061 atggtctggt gaaatactgg cttaccttca atgaaatcaa cattatgttg catagcccgt
     9121 tctccggcgc gggtctggtg tttgaagaag gtgaaaatca ggatcaggtg aaatatcagg
     9181 ccgcgcatca ccagctggtt gccagtgcgc tagccaccaa aatcgcccat gaggttaacc
     9241 cgcaaaatca ggtgggctgt atgctggcgg gcggtaactt ctacccttac agttgcaagc
     9301 cggaagatgt ctgggcggcg ctggagaaag accgggaaaa cctgtttttt atcgatgtgc
     9361 aggcgcgggg cacgtatccg gcttactctg cccgcgtatt ccgcgaaaaa ggggtaacca
     9421 tcgacaaagc accgggcgat gatgaaatcc tgaaaaacac cgtcgatttt gtctctttca
     9481 gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc caacaatagc agtgcggcga
     9541 acgtagtgaa atcgctgcgt aacccgtatc tgcaagtgag cgactggggc tggggtattg
     9601 atccactcgg tctgcgtatc accatgaaca tgatgtacga ccgttatcag aagccgctgt
     9661 ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc tgccaatggc gagattaacg
     9721 atgactatcg catcagctat ttacgcgaac atatccgcgc aatgggcgaa gcgattgcag
     9781 atggcattcc gctgatgggc tacaccacct ggggctgtat tgatttagtc tccgcctcta
     9841 cgggtgaaat gagcaaacgc tacggttttg tctacgtaga ccgtgacgac gcaggcaacg
     9901 gcacgctgac gcgcacacgt aagaaatcgt tctggtggta taaaaaagtg attgccagta
     9961 atggggaaga tttagagtag gtaacagtgc cggatgcggc gtgaacgcct tatccggcct
    10021 acatgtcgca cgaaccagga aggcccgata agcgcagcgc atcgggcaat ctggggtttg
    10081 gcatcagtca aaatcaaaga aaccattccg gcttaacgcc aaatcgggct gaaagtgcct
    10141 taatgtggga tatcgtcagg gatcgttgtc cagacaaaat ctgactgacc agggatttag
    10201 agccgatttc gttcttcaaa tccgcatagg tcagattatg ttgatcaatc agcgtgcgaa
    10261 gtaacgccac gccaacaggc attgccgcaa cggctttgtc gaactctgca aactcttggt
    10321 tgttgttttc atattctgca atgcggctcg ccagaaagtc gataagtgga ttggtgtcat
    10381 cttcttcaat caaatattcg accagttcca gtgcttcacg ataatcatct tctgagtcac
    10441 tacctcccag aaaggggact gcattgacca gttctcttgt tgctttaaca gcgcgtgcag
    10501 cattagcagt cattctttgt tctctctgta gtaacgtgtc agtttgtcat actcggcgtg
    10561 attagttatg tgcttcacaa agaaccgctt attaacaaag tttatgtagg caatcaccct
    10621 taggttgttc ccacctacat ccagaacgta ccatttgttg cgatatttaa aattgtcgag
    10681 gttgggaaac gccgttcgca tctcttctgg cgtagaaaaa tccgtttcct taataacccg
    10741 atacaatgcc tgaagagcca gagcgtcgtt aggatattta cgcgcagact cttcaaatgg
    10801 cgctttagaa atgatgtgca tttttaagtt ttaacttcct tatagtaagt aaacagccac
    10861 atctgcgagt tctatcctta tgtgttcgga aactatagct catcaaatcc aagttcacaa
    10921 tatgtaaaca cttgagcgcg atataattac ccccaaaatc ctcatagcca ctctgcaaaa
    10981 agtcagttct ggttcacccc atcaagaaca tcccccttta tcaagcaacg ctgcgtctgc
    11041 gccagatctt ccgtttgcgg gtcgcgcccg gcgatgggtt tgaggacgag tttacggttg
    11101 ggggtataag taaaacgcgt gagcgcggga tagcttaact tgccaaacca gccgtgaaca
    11161 cgctgccatt tggcgtcttt tacctgatac agactggtta tccagtaacc ggaatcaaac
    11221 tgcatatcca gcaattgcgt gtgaccattg ccttgcaaat ccagcaaatc atctacgcca
    11281 gttttgcttg cggtgtagaa tcccctgggt tcaaagacac agggacggcc ttcgcgcgtg
    11341 aacgtcataa gaattaaatg tgcataagga gccagaccat taccggtact tggtaaccag
    11401 ataaccagat cctgaatgcc gttacgatca agatcggcgc gatagagttg ccctttgccc
    11461 gctatgtttg ccagcatacc cacaggcaca gaccaggatt taccggcgcg atctttaccg
    11521 ttcaattgcc attcactatc atcatttggc atcttttcaa gcgtcacgcc agcaacgccc
    11581 acttttctaa aatgccaggt ttgctcggca tcataaggca tggtcaggag aggtttcagc
    11641 gcccgctttt gcgcgtattc gctgtcactc attggcagat attcggtaaa gctggtggcc
    11701 tgtgcaggca gcactaagct caataaaaaa gcgcacatcc ctgttctgat attcatttcg
    11761 ctgaattccc tgatggagaa ttattcttct tccaccgcta actgcgcgaa gccgccattc
    11821 ccttcccagc cttccagtcg ttgataaacg gtttctaccg catctttaat cggctgagtc
    11881 atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac ttcgccaatg
    11941 tcttctttca actggtcgat aagatagttc agcggcatgt tatgggtagt catcataaac
    12001 atctcggcga tatcatccgg gtcgatgatg cggatttcgc cggggtttag ccccatatca
    12061 gtagcgtcaa caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac gatgtcgttt
    12121 tccggtgcac taccgccatc aatcaccacc cagttacctt tcggcgcggc ggcgcacttt
    12181 tccgccagca gcgggccagc gccatcatcg cccatcatgc tattgccaac acagagtaaa
    12241 acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat gaatatcatg
    12301 cagcatactt aacagcattg tactccattc cttctgctgc gaagtttgcg ctgcccgggc
    12361 tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga tctcaccata
    12421 tttcggcacg ccttccattt tgcggcgggc ttcactgccc gcctccagag tggcaatcca
    12481 tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga taaccccaag
    12541 gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg gcgtggcatc
    12601 gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac tcatcggata
    12661 cgcgcctctt caacaacatg attcagatgg ctgacaatct cgttcagacg cggatcgttt
    12721 tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt caggaagtca
    12781 tcggcaatct gacgaccgta acgataaccc gccaggcggc gtgcttcgcg atccactttc
    12841 acacgcagcg gctgcaccat atcgccatgc aggatttccg cctgttgttc atcctgctca
    12901 cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc aaagccgtac
    12961 agcgtggcgg caggcgttgg cgggcaacca ggaatgtaga catctaccgg gacgatttta
    13021 tccgtaccgc cccacacgca gtagagatcg tggaaaatac caccgctgtt accgcaggca
    13081 ccgtaggaga tacaaatttt cgggtccggc gcggactgcc acgcacgcag cgcaggggat
    13141 cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg cggtgaagga
    13201 acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc gaaaatttcg
    13261 atttcgcagc cgttgcagcc gccgcagtcc acgcgataaa cgtaggcaga acgtttgatt
    13321 tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat cgggaccgga
    13381 atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct ttcatatggc
    13441 gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat tccgggcaag
    13501 tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcgcc gttgtgctta agcagcgcaa
    13561 tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcgg cagttgcaca
    13621 gcgcgaagcg ggactgttgc aggaagtctt ctttcttcca cactgccagt tcgtactctt
    13681 gcgacagttt gatcgccgcc gtcgggcaaa cttcttcgca gcgtccacag aagatgcagc
    13741 gcccgagatt aaactcccac gccagctcac cggtggcgag gtcagtttca accgttaagg
    13801 cgtttgacgg gcaggcattg acgcaggccg cacagccgat gcactgctgc gggttctgct
    13861 ccggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa gacgaggtcg
    13921 ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct tatttcagcg
    13981 gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc actttgctct
    14041 ttttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa gggtccaagc
    14101 taccgataat cagcggcgca tcggaaacgg tgttaccgcg cagcatgtag cgcagggtcg
    14161 gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg ttgtcgccgg
    14221 tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc gcgaaacggt
    14281 gcggaatgta ggtaaagcct tccaccatca gcggaccgcc cggcaggtta tccagaccgt
    14341 agtcgatcat gttcagcgcg gtataaactt cgttgatacg cactttcaga cgggaaataa
    14401 cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataaccg acaaacgggt
    14461 gatcggcgcg agtatcgcgg gcgtgaccgc tggcgcgaac catcgggcca acgttactga
    14521 aatcgcgggc catttccggg tccagacgac caatgccgac ggtgcgctgt tccatgttcg
    14581 gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatctgt tgcgccagct
    14641 ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg aatcccgccg atcaggttca
    14701 ggccgtaggt tttacgcgca ccggtgagga tctctgccat tttcatggag gtttcacgca
    14761 cgcggaagaa ctgcataaag ccggagtcaa agccggtaaa gtggcaggcg agaccgaggt
    14821 tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc atctgcgcac
    14881 gttctggcac ctgaataccc atcgcgtttt ctaccgacgt ggtgtaggcg gtgctgtggg
    14941 caaagccgca gatcccgcac acacggtcag agaggaaggt cacttcgttg taacccatac
    15001 gggtttccgc cagtttttcc atgccgcgat gaacgtagaa cagacggtag tcggcgtcga
    15061 taatgttttc gccatcgacg aacagacgga agtggcccgg ttcgtcagaa gtgacgtgca
    15121 gcggaccaat cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg
    15181 tttcagcatc ggtggtcggt gccggacgct gacgataatc catgctgtct ttacgcagcg
    15241 gataaagttc atccggcagc accagacggc gttcatccgg cagaccaacc ggaatcaaac
    15301 cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc ggcgtcacgg
    15361 acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag cacttagtcc
    15421 ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg cgttcgtcgt
    15481 taccaaaaag caccgacaac cagcccccct gcttgtagta gagaaactcc accacttccg
    15541 gcagatagtt cacctttacg gtgacagtca gctgatcttt ggtctgccag gcgtggtcca
    15601 gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct aatttttctt
    15661 cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg ctaaaaatgc
    15721 aaagccaaac ccagtccagg taatgcgcgg agtaatatca agacgcagac gggccatgct
    15781 gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg caatcaccag
    15841 cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga taaatacgcc
    15901 gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca tgacgccaaa
    15961 gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg cttccgccag
    16021 gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac aaagcgccag
    16081 taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga tgttgctgat
    16141 gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca tcggttcgac
    16201 cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc taccggtatc
    16261 cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta aggtgattaa
    16321 atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag tagcgatagt
    16381 cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg aggcatccgg
    16441 gccaacgctc tggcggccca gcagtttgaa aatgtcacga tactcctgca acacacccgg
    16501 cccacgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga gcagcggcgc
    16561 aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc tcaggctcct
    16621 cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg gcggaacagc
    16681 aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag ccatttgcgc
    16741 agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg agcggtaatc
    16801 accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga acgtgacggc
    16861 aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca ggcaatcagc
    16921 agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg cagaggtaca
    16981 gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac gcagcaaatc
    17041 gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt ttcggcttct
    17101 ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca catcaccgcc
    17161 agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg ggcaacaaac
    17221 gcgccgctat tgctcagttt gaagaaggat tgatagataa cccattcccc ggcaaacccg
    17281 ttcagtggcg gcagcgcagc cattgccatc aacccgacta acatggcgat ggagataacc
    17341 ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt acggaaccag
    17401 acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag cagatggtac
    17461 agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat acccgttacg
    17521 ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta agccagcagg
    17581 cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt gatcatgccg
    17641 agcaccagca gcgcgatccc ccaccacagc ggtgcattac cgcccagcag tgacagggtt
    17701 aacatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc agctggcgca
    17761 gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc ggccagcagg
    17821 ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg ttgcatacgc
    17881 atatccagta ggcacagatc cagcgtgcca taacgctgcc acagcagcca gcaagcaatc
    17941 accagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc tttgctgttg
    18001 ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa catgccgagg
    18061 ttgctggcga tgacggcgca gacggcggca gccatcaaca tattgatctg caagccgttg
    18121 cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa cagaccgcac
    18181 agaccgagcg taatcagcca aatcgcgtta agcggagaga tttgcacatc gtagcttacc
    18241 agcgacagcg caccgctcac gccaaccgcg ccagtcagta cagtgaagcc cgcggctgcc
    18301 gtatacagac taccaaccgc gccgccaatt ccagctatcc agccacttaa cgctttttga
    18361 aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc atgccacgcc gctattgatc
    18421 agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag gtgagatcgc
    18481 caaagtccgt gttaaaggtc agctcacgct tacgtttgct gacgcgggcg atatcggtgt
    18541 tatccaccag atgcagggct ttagtcgggc acatccgcac gcaggccgga ccttgttcat
    18601 caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc cagtcaagca
    18661 gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta ttggcgtttg
    18721 ccggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg caggcgatgc
    18781 cgcacagttt gcagcttacg cacagacttt cattcaactg cacggcccca tcaatgcggg
    18841 tgatggcgtt aaccgggcaa accaccgcac agggtgcatc ttcacagtgg tgacagagct
    18901 gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat tgcaggccgt
    18961 gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata cagagcgtgg
    19021 agtcaacaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat ttttgacgaa
    19081 aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc aaacaggcgt
    19141 aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc ctttaccagt
    19201 tggcttaaat tcaccggcac attgttttca atagcggtgt ataagctgtc gtaaacgcca
    19261 gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt gaccttgcca
    19321 tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc ttcgccatct
    19381 tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag ggtgacgtaa
    19441 atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca gctcatggcg
    19501 tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca gtagatgtgc
    19561 cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc gcttatttcc
    19621 caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc attttctatg
    19681 ccaactttta atttattgtt attaaataga tttttaagct aaagatgaat ttcgtcgccg
    19741 tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat caacactggc
    19801 acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga aataaccctc
    19861 tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc aaaacgcgta
    19921 actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc tcttgccttt
    19981 tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca cctcgaagaa
    20041 caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact gacccagcgc
    20101 gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga cgacggttta
    20161 cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg cggttgcggt
    20221 gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt tcgtagcgcg
    20281 ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc gcctgaattt
    20341 acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc gggcactcac
    20401 gcgccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct ggacaaaaat
    20461 aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact ggtgctcaac
    20521 ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaaccct aatgcgcctg
    20581 aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa cgatgccgca
    20641 cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg ctgccatctt
    20701 gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa cggtattctg
    20761 tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg tgaaaaacac
    20821 aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata tccgcatatg
    20881 tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta tctcaacttt
    20941 gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga aatcatcctt
    21001 atttccgcca ccagcggcga agggatggac cagtggctga actggctgga gacacagcga
    21061 tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca aaagtcgacg
    21121 tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat gaaaacggtc
    21181 agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc gtaattaatg
    21241 aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt gagccggatg
    21301 tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat atcgcgcgcc
    21361 ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc tctcttacac
    21421 cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg ccatttttaa
    21481 attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc cgggttgccc
    21541 ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca gccatccgga
    21601 agtcatcttc tgtacctttg gcgacgccat gcgcgtgccg gggaaacagg gatcgctgtt
    21661 gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg atgcgttgaa
    21721 actggcgcag gagaatccaa cccgtaaagt cgtattcttc ggcttaggtt ttgaaaccac
    21781 catgccgacc accgccatca ccctgcaaca ggcgaaagcc cgcgatgtgc agaattttta
    21841 cttcttctgc cagcatatta cgcttatccc gacgctgcgc agtttgctgg aacagccgga
    21901 taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg gcactgatgc
    21961 ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt tcgaacccct
    22021 tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc acagcaaggt
    22081 agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc aacaggcaat
    22141 tgctgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg tgattgaatc
    22201 ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac atttccgccc
    22261 agcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat taacgggcaa
    22321 atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa ccgcatttgg
    22381 tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc gtcagcagga
    22441 gaatgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc gatgcagcaa
    22501 ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga gcaggaagac
    22561 caggcacgtc ttgatctggc gcagctggta gcagaaggcg accgtctggc gttctccacc
    22621 gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa gctggcgatt
    22681 tgcggcacag ccaatgacgt tgcggtcagt ggcgctattc cgcgctatct ctcctgtggc
    22741 tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac cagcatggca
    22801 gaaaccgcgc gcgcggcagg cattgccatc gttactggcg atactaaagt ggtgcagcgc
    22861 ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc ggcgaatatt
    22921 cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg cacgctcggg
    22981 gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg cgagctggtc
    23041 agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc cggcgtgaaa
    23101 gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt cgcggcagcc
    23161 tgcggttgtg gtattgaaat ttcagaagtg gcactgccgg ttaaacctgc cgtgcgcggc
    23221 gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa actggtgatc
    23281 gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca tccactgggg
    23341 aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct tgccggtctg
    23401 tatggtgtga aacgaaccct cgatttacca cacgccgaac ctcttccgcg tatatgctaa
    23461 taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg cggtgctttc
    23521 ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag ggttgttcga
    23581 catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg ctctttcgca
    23641 actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc aagcgcagac
    23701 tcaacgtgcg tcttattacg catcgcgtga aaaagacacc ccattaaata tgaagacgaa
    23761 actgttctgg cacacggtcc ggtacgccgc attttgtcgc gccctgatac gctgcattgc
    23821 agttacgaag aattttgtga aacctggccg cagctggtcg caggtgggct ataccccaaa
    23881 tttggtcact attgcctgat gccactggcg gcggaagggc atatttttgg tggctgtgaa
    23941 tttattcgtt atgacgatcg tccctggagc gaaaaagagt tcaatcgtct gcaaacattt
    24001 acgcagatcg tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa caatgtcgac
    24061 tatgagttgt tatgccggga gcgcgataac ttccgcatcc tggttgccat taccaacgcg
    24121 gtgctttccc gcctggatat ggacgaactg gtcagcgaag tcgccaaaga aatccattac
    24181 tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc accgtaaaaa caaactcaac
    24241 atctactcca ctcactatct tgataaacaa catcccgccc acgaacagag cgaagtcgat
    24301 gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag agatgctgct gatcaatctc
    24361 cacgagcggg acgatttagc cccctatgaa cgcatgttgt tcgacaccta gggcaaccag
    24421 attcaaacct tgtgcctgtt accgctgatg tctggcgaca ccatgctggg cgtgctgaaa
    24481 ctggcgcaat gcgaagagaa agtgtttacc actaccaatc tgaatttgct gcgccagatt
    24541 gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct atcaggaaat ccatcgtctg
    24601 aaagaacggc tggttgatga aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt
    24661 gaatttggcg agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa
    24721 atggtggcgc aaagtgacag taccgtgctg atcctcggtg aaactggcac gggtaaagag
    24781 ctgattgccc gcgcgatcca taatctcagt gggcgtaata atcgccgcat ggtcaaaatg
    24841 aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc tgtttggtca tgagcgtggg
    24901 gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg aactggcgga taaaagctct
    24961 ctgttcctcg acgaagtggg cgatatgcca ctggagttac agccgaagtt gctgcgtgta
    25021 ttgcaggaac aggagtttga acgtctcggc agcaacaaaa tcattcagac ggacgtacgt
    25081 ttaattgccg cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga gttccatagc
    25141 gatctctatt accgcctgaa cgtattcccg attcacctgc cgccactacg cgagcgtccg
    25201 gaagatattc cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc
    25261 aatatcgaca gcattcctgc cgagacgctg cgcaccttga gcaacatgga gtggccgggt
    25321 aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat tgctaacacg cggtaacgtg
    25381 ctgcagctgt cattgccaga tattgtttta ccggaacctg aaacgccgcc tgccgcaacg
    25441 gttgtcgccc aggagggcga agatgaatat cagttgattg tgcgcgtgct gaaagaaacc
    25501 aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc
    25561 ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg cattgattta actgcaaatt
    25621 gccggacaga tctgcctgtc cggcatacta ttcacgaggt tttttcggac gatatttttc
    25681 cggcagttct ggtaccggac gcttgtcatc gatgagatga cgcacggtta agatcggatg
    25741 acgccacagc attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc
    25801 aggctggtaa cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca
    25861 tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga
    25921 cgcctgcggg catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg
    25981 agagatacgc ttgccggaca tgctgcctcc atactcttaa ggtgtattta tattacaact
    26041 taatttgaaa ggggactatg gctctgaagg aggaggttta ttcaaccgac gcaattttgc
    26101 gtacttgctt cataagcatc acgcaaaagc tgcaaaacag catctttccc ggaaccagca
    26161 tcaagaactc gccgttcgct tcttcccctg aaatgattaa ctccggtatc atgtgcgcct
    26221 tatgtgatta caacgaaaat aaaaaccatc acaccccatt taatatcagg gaaccggaca
    26281 taaccccatg agtgcaatag aaaatttcga cgcccatacg cccatgatgc agcagtatct
    26341 caggctgaaa gcccagcatc ccgagatcct gctgttttac cggatgggtg atttttatga
    26401 actgttttat gacgacgcaa aacgcgcgtc gcaactgctg gatatttcac tgaccaaacg
    26461 cggtgcttcg gcgggagagc cgatcccgat ggcggggatt ccctaccatg cggtggaaaa
    26521 ctatctcgcc aaactggtga atcagggaga gtccgttgcc atctgcgaac aaattggcga
    26581 tccggcgacc agcaaaggtc cggttgagcg caaagttgtg cgtatcgtta cgccaggcac
    26641 catcagcgat gaagccctgt tacaggagcg tcaggacaac ctgctggcgg ctatctggca
    26701 ggacagcaaa ggtttcggct acgcgacgct ggatatcagc tccgggcgtt ttcgcctgag
    26761 cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa cgcactaatc cggcggaact
    26821 gctgtatgca gaagatttcg ccgagatgtc gctgattgaa ggccgtcgcg gcctgcgccg
    26881 tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag cagttgaatc tgcaatttgg
    26941 cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg cgcggacttt gtgctgccgg
    27001 ttgtctgttg cagtatgcga aagataccca acgcacgacc ctgccgcata ttcgttctat
    27061 caccatggaa cgtgagcagg acagcatcat tatggatgcc gcgacacgtc gtaacctgga
    27121 aattactcag aacctggcgg gcggtgcgga aaatacgctg gcttctgtgc tcgactgcac
    27181 cgtcacgccg atgggtagtc gtatgctgaa acgctggctg catatgccag tgcgcgatac
    27241 ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg caggatttca ccgccgagtt
    27301 gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt ctggcacgtc tggctttacg
    27361 aactgctcgc ccacgcgatc tggcccgtat gcgccacgct ttccagcaac tgccggagct
    27421 gcgtgcgcag ttagaaactg tcgatagtgc accggtacag gcgctacgtg agaagatggg
    27481 cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc atcgacacac cgccggtgct
    27541 ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa gagctggatg agtggcgcgc
    27601 gctggctgac ggcgcgaccg attatctgga gcgtctggaa gtccgcgagc gtgaacgtac
    27661 cggcctggac acgctgaaag ttggctttaa tgcggtgcac ggctactaca ttcaaatcag
    27721 ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt cgccagacgc tgaaaaacgc
    27781 cgagcgctac atcattccag agctaaaaga gtacgaagat aaagttctca cctcaaaagg
    27841 caaagcactg gcactggaaa aacagcttta tgaagagttg ttcgacctgc tgttgccgca
    27901 tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa ctcgacgtgc tggtgaacct
    27961 ggcggaacgg gcctataccc tgaactacac ctgcccgacc ttcattgata aaccgggcat
    28021 tcgcattacc gaaggtcgcc atccggtagt tgaacaagta ctgaatgagc catttatcgc
    28081 taacccgctg aatctgtcgc cgcagcgccg tatgttgatc atcaccggtc cgaacatggg
    28141 cggtaaaagt acctatatgc gccagaccgc actgattgcg ctgatggcct acatcggcag
    28201 ctatgtaccg gcacaaaaag tcgagattgg acctatcgat cgcatcttta cccgcgtagg
    28261 cgcggcagat gacctggctt ccgggcgctc aacctttatg gtggagatga ctgaaaccgc
    28321 caatatttta cataacgcca ccgaatacag tctggtgtta atggatgaga tcgggcgtgg
    28381 aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc gcagaaaatc tggcgaataa
    28441 gattaaggca ttgacgctgt ttgccaccca ctatttcgag ctgacccagc taccggagaa
    28501 aatggaaggc gtcgccaacg tacatctcga tgcgctggag cacggcgaca ctatcgcctt
    28561 tatgcacagc gtgcaggacg gcgcggcgag caaaagctac ggtctggcgg ttgctgctct
    28621 ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa aaactgcgtg agctggaaag
    28681 catttcgccg aacgccgccg cgacgcaagt ggatggtacg cagatgtctt tgctgtcagt
    28741 gccagaagaa acttcgcctg cggtcgaagc actggaaaat ctcgacccgg attcactgac
    28801 tccgcgtcag gcgctggagt ggatttatcg cttgaagagt ctggtgtaat aacaattccc
    28861 gatagtcttt tgctatcggg aatattaacg acaactgacg aatcaaataa aaataccctg
    28921 tataatagga aagcttattt tacagggtaa aaccatgcca tctacacgct atcaaaaaat
    28981 caatgcccat cactatcgcc atatatgggt cgttggtgat attcatggtg aatatcagtt
    29041 attacaatcc cgttaacatc aactctcttt ttaccctgaa accgacttac ttatttccac
    29101 aggcgataat attgatcgtg ggccgaagag tcttaacgtg ctgcgcctgc taaaccaacc
    29161 ctggtttacg tcggttaaag gcaaccacga agcgatggcg ttggatgcat tcgaaactgg
    29221 cgatggaaat atgtggcttg ccagcggtgg tgactggttt ttcgatttaa atgattcaga
    29281 gcaacaagag gcaatagatc tgttgctgaa attccatcac cttccacata ttattgaaat
    29341 cattaatgac aacataaaat atgccatc
//
LOCUS       DAZOZY010000030        29113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZOZY010000030 DAZOZY010000000
VERSION     DAZOZY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.030"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..869
                     /locus_tag="ACHURQ_001175"
     CDS             <1..869
                     /locus_tag="ACHURQ_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0823008.1"
                     /translation="NLLVIFECIYQHLSISKAAESLYITPSAVSQSLQRLRAQFNDPV
                     FIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFIIYGPQLISCSNNS
                     MLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVISRSVICMPLHTIR
                     NTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEKFMNRKISFRGSS
                     LLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLYISYNKSSLNNLV
                     FSRFIDRLNESF"
     gene            1016..2236
                     /locus_tag="ACHURQ_001176"
     CDS             1016..2236
                     /locus_tag="ACHURQ_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0823009.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2209..2838
                     /locus_tag="ACHURQ_001177"
     CDS             2209..2838
                     /locus_tag="ACHURQ_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR0823010.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2839..3999)
                     /gene="ybdL"
                     /locus_tag="ACHURQ_001178"
     CDS             complement(2839..3999)
                     /gene="ybdL"
                     /locus_tag="ACHURQ_001178"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR0823011.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4108..5196
                     /gene="hcxA"
                     /locus_tag="ACHURQ_001179"
     CDS             4108..5196
                     /gene="hcxA"
                     /locus_tag="ACHURQ_001179"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR0823012.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5206..5403)
                     /locus_tag="ACHURQ_001180"
     CDS             complement(5206..5403)
                     /locus_tag="ACHURQ_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0823013.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5585..7690)
                     /gene="cstA"
                     /locus_tag="ACHURQ_001181"
     CDS             complement(5585..7690)
                     /gene="cstA"
                     /locus_tag="ACHURQ_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR0823014.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7871..8284)
                     /gene="entH"
                     /locus_tag="ACHURQ_001182"
     CDS             complement(7871..8284)
                     /gene="entH"
                     /locus_tag="ACHURQ_001182"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR0823015.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8287..9033)
                     /gene="entA"
                     /locus_tag="ACHURQ_001183"
     CDS             complement(8287..9033)
                     /gene="entA"
                     /locus_tag="ACHURQ_001183"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR0823016.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9033..9890)
                     /gene="entB"
                     /locus_tag="ACHURQ_001184"
     CDS             complement(9033..9890)
                     /gene="entB"
                     /locus_tag="ACHURQ_001184"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR0823017.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9904..11514)
                     /gene="entE"
                     /locus_tag="ACHURQ_001185"
     CDS             complement(9904..11514)
                     /gene="entE"
                     /locus_tag="ACHURQ_001185"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR0823018.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11524..12699)
                     /gene="entC"
                     /locus_tag="ACHURQ_001186"
     CDS             complement(11524..12699)
                     /gene="entC"
                     /locus_tag="ACHURQ_001186"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR0823019.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13074..14039
                     /gene="fepB"
                     /locus_tag="ACHURQ_001187"
     CDS             13074..14039
                     /gene="fepB"
                     /locus_tag="ACHURQ_001187"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0823020.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSVLALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(14120..15370)
                     /gene="entS"
                     /locus_tag="ACHURQ_001188"
     CDS             complement(14120..15370)
                     /gene="entS"
                     /locus_tag="ACHURQ_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR0823021.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15481..16485
                     /gene="fepD"
                     /locus_tag="ACHURQ_001189"
     CDS             15481..16485
                     /gene="fepD"
                     /locus_tag="ACHURQ_001189"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR0823022.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16482..17474
                     /gene="fepG"
                     /locus_tag="ACHURQ_001190"
     CDS             16482..17474
                     /gene="fepG"
                     /locus_tag="ACHURQ_001190"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR0823023.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17471..18286
                     /gene="fepC"
                     /locus_tag="ACHURQ_001191"
     CDS             17471..18286
                     /gene="fepC"
                     /locus_tag="ACHURQ_001191"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0823024.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18283..19416)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHURQ_001192"
     CDS             complement(18283..19416)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHURQ_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR0823025.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19632..23513)
                     /gene="entF"
                     /locus_tag="ACHURQ_001193"
     CDS             complement(19632..23513)
                     /gene="entF"
                     /locus_tag="ACHURQ_001193"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR0823026.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(23510..23728)
                     /gene="ybdZ"
                     /locus_tag="ACHURQ_001194"
     CDS             complement(23510..23728)
                     /gene="ybdZ"
                     /locus_tag="ACHURQ_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR0823027.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(23731..24933)
                     /gene="fes"
                     /locus_tag="ACHURQ_001195"
     CDS             complement(23731..24933)
                     /gene="fes"
                     /locus_tag="ACHURQ_001195"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR0823028.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25176..27416
                     /gene="fepA"
                     /locus_tag="ACHURQ_001196"
     CDS             25176..27416
                     /gene="fepA"
                     /locus_tag="ACHURQ_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR0823029.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27591..28211
                     /gene="entD"
                     /locus_tag="ACHURQ_001197"
     CDS             27591..28211
                     /gene="entD"
                     /locus_tag="ACHURQ_001197"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR0823030.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28333..28485)
                     /gene="hokE"
                     /locus_tag="ACHURQ_001198"
     CDS             complement(28333..28485)
                     /gene="hokE"
                     /locus_tag="ACHURQ_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0823031.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(28836..28988)
                     /gene="hokE"
                     /locus_tag="ACHURQ_001199"
     CDS             complement(28836..28988)
                     /gene="hokE"
                     /locus_tag="ACHURQ_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR0823032.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT         7057 a         8081 c         7452 g         6523 t
ORIGIN      
        1 taaatcttct tgtcatattt gagtgtattt accagcattt aagcattagc aaagctgctg
       61 aatcactata tatcacccca tcagccgtga gccagtcgtt acaacgctta cgtgcgcaat
      121 ttaatgaccc tgtattcatt cgctcaggaa aaggtatcgc gccgacgaca acagggttga
      181 atctgcatca ccatcttgag aaaaacctca aaggccttga acaaacaatt aatatagtca
      241 ataagtccga actaaagaaa aactttatta tctacggccc tcagctcatt tcttgctcta
      301 ataatagtat gctgatccgc tgcctacggc aagatgcctc tgtcgagatt gagtgccatg
      361 atatcctcat gtcagcagag aatgctgaag aactgttggt tcaccgcaaa gccgatctgg
      421 tgatcaccca aatgccagtg atcagccgtt ccgtcatttg catgccattg catacaattc
      481 gcaatactct aatttgtagt aataagcatc cgcgaattac tgacaatagc acttatgaac
      541 aaattatggc agaagagttc acccaattaa tatccaaatc agcaggagtt gatgatattc
      601 aaatggaaat agacgagaag tttatgaatc gcaaaatttc atttcgtggt tcctctttgc
      661 tgacaataat taatagcatt gcagttacag atttactggg aatagtcccc tatgaacttt
      721 ataactccca ccgtgacttt cttaatttaa aagagataaa actagaacat ccgttacctt
      781 caataaagct ttacatttcc tataataaat cttctttaaa taatttggtg ttctcccgat
      841 ttattgaccg tctaaatgag agtttttgat atataactac agacagctac tataactttc
      901 atctatttat tcacagcgcg aatgaaaaat aaaacactct gctttacagt gtttacttta
      961 taattaacag tcttatctca ctgttcaccc tgtaacgttc agagagcgtc ttcagatgtc
     1021 tatttataaa attcctcttc cgctcaatat tctggaagct gcacgagagc gtattacctg
     1081 gacactcaac accctacccc gcgtttgtgt ctcattttct ggcggcaaaa actctggttt
     1141 aatgctgcat ctgacagccg aacttgcccg acaaatgggc aaaaaaatct gcgttttgtt
     1201 tatcgactgg gaggcgcaat tctcttgcac cattaactat gttcagtccc tgcgcgagtt
     1261 gtacaccgat gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc
     1321 cctttcacaa taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca
     1381 gcccccgcaa gatgcgataa ccgatcctga ctttttctgc ttttaccagc caggcatgac
     1441 cttcgaacaa tttgtacgtg agtttgccga atggttttca caaaaacgtc cggtggcgat
     1501 gatgatcggc atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa
     1561 taaacaacgt tttgccgacg ataaaccatg gactacggcc gcaccaggcg gtcatagctg
     1621 gtacatttac cccatttacg actggaaagt ggctgatatc tggacctggt atgcaaatca
     1681 tcaaaatctc tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca
     1741 tatgcgaatt tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt
     1801 tatcgaaccg gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg
     1861 catttacgcc ggacatgata atcatttcta tgggcatcgg aaaatcctca agccagaaca
     1921 tttagactgg caagaatatg cattattgct gctcaatagc atgccggaaa aaacagctga
     1981 gcattaccgc aataaaatcg ccatttattt gcactggtat cagaaaaaag gcatcgaggt
     2041 tccacaaacc cagcaagggg acattggcgc gaaagatatc ccctcctggc ggcggatatg
     2101 caaagtttta ctcaataacg attactggtg tcgggcatta tcatttagtc ctacgaaagc
     2161 gaagaactat cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact
     2221 atgcaacaac gattaacgca ggatttaacg caatttctcg ccagcttgcc ggaagatgat
     2281 cgcatcaaag cgattaatga aatccgcatg gcgatccatc aggttagccc ctttcgtgaa
     2341 gagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa tgattacaac
     2401 ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga aatagatggt
     2461 tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat cgtggatggt
     2521 tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt aaaaggttat
     2581 ttgccggtga tctgtctgga aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat
     2641 aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg tgaacttagc
     2701 cagttgggat gggacgataa taaaattggc aaagagctgg gaatggacag cgacgaagtg
     2761 ctgcgcctga agcaaattaa cggcctgcaa gagctgtttg ccgaccgtca atattcccgc
     2821 gcctggacgg ttaaataact aaagctggcg caggcgttca gctgctgcca gcaacgtcga
     2881 ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg cgcaaaacac
     2941 cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa actcaacatc
     3001 atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt cacacggtaa
     3061 aatctccagc cggctttcat ttaaggcatt taccagaata tcgcgcttct ggcgataaaa
     3121 gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa gcgccagctg
     3181 tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt cggcgctgat
     3241 gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg tcttgccaaa
     3301 tgaagaaacc gccactgccc gctcacgcag ctgcgaatgc gccagcacac tggcatggcc
     3361 ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc
     3421 ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg cactggagtt
     3481 atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg caaattcctg
     3541 ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgc ttcactattc ccccagaaag
     3601 cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt catcgccatt
     3661 gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg ttacggtgat
     3721 atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag caatcgcctc
     3781 gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg caacgtggta
     3841 cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt gcgacaggtt
     3901 aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg tgccaagttg
     3961 tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac ctgacgaagt
     4021 tgcgtgttga aggcactata acacgatgct agtatttggc aatcaagacg tttagatgtc
     4081 taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt ggtcgtcggc
     4141 ccggctaact acttttcaca tccaggaagt ttcaatcacc tgaacgattt tttcactgat
     4201 gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc ggcgcaaacc
     4261 aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg tggtcattgc
     4321 agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag cgtggtgatt
     4381 ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg tctcggtctg
     4441 ccatttgttg ccgttccgac gatcgccgct acctgcgccg cctggacacc gctctccgtc
     4501 tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc caattttatg
     4561 gtgctggtgg aaccagagat tatcctcaac gcgccgcaac aatatctgct ggcggggatc
     4621 ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc agaaacgttg
     4681 ccgctaaccg tgcgactggg gatcaataat gcgcaggcca ttcgcgacgt attgttaaac
     4741 agtagcgaac aggcgctgag cgatcagcaa aatcaacagt taacgcaatc attttgcgat
     4801 gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga tcgttttacg
     4861 cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca aaccgagaaa
     4921 tttctccacg gcaccaaagt cgcttatggc attctggtac aaagcgcctt gctaggtcag
     4981 gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc gaccacgctg
     5041 gcggagctgg aaatggatat caataatcat gcggagatcg acaaagtgat tacccacacc
     5101 ctgcgtccgg tggaatacat tcattacctg ccagtcacgc tgacaccaga tacgttgcgt
     5161 gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc agcagcgcgc
     5221 gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactctt cgtaggtcat
     5281 cggcgtttga tcgggatggt taacccgcat atgttcgaca tagttgtcgt aatcaggcat
     5341 accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca gtgaatcaaa
     5401 catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt gaacgcctta
     5461 tccggcctac aaatgctatc aacgcgctcg gtcagtcccc tcgccccatc ggggagaggg
     5521 ttagggtgag gaggaaccct cggcactatg ccaacattcg ccaacatccc ccctcactct
     5581 gactttagtg tgcgcctttt gcctgcgcca cgatctcctc gacattttcc ggcattggct
     5641 catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgccagt gccgacttaa
     5701 tcgagaacag cgccagaacc accacgacca ccataaagaa gatggttaac ccggcatcca
     5761 gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac ggaatattgc
     5821 cgctgtcgat cattgcctgg aacttattag caatggccag gaagccgact ttcgcatccg
     5881 ggctaaacgc tttctgccag cctgcggtca gggtacaaat cagcagccag gccgttggta
     5941 ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg gcacagagca
     6001 tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac agagtgttaa
     6061 tgccgcccag cggatcgacc acgccctgat ggaggaagta gccccacgcc agtacgcaca
     6121 gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca ggagacacca
     6181 cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct accgccgtca
     6241 gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca tccatcatac
     6301 cacccagcgc accgtgcagg atgtaagcca tccccaccgc cagcgtcggc gcaccgcccg
     6361 cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc gtatccggag
     6421 taatcgcaaa gccccagcta ctcaccacct gcgcggcaga agcgaccaca tccgccgtcc
     6481 ctgccggagc cagcaccgcc atcgggctgt tcatggcgaa gtacacgccc ggatcgatga
     6541 tacaggcgga aaccagtgcc ataatcgcca cgaaggattc cattaacatt ccaccgtagc
     6601 cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg gaagagatca
     6661 gcgcatggaa gcctgacacc gcgccacagg cgatggtgat aaacaggaac gggaacagat
     6721 taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc atggtcagcg
     6781 tcggacgcat aatcaaaatg cctaccgcca gaccaacgat agtcccgatt ttcaggaagg
     6841 tagagaggta gtcacgcggg gccagcagta accacaccgg cagcaccgcc gccacaaaac
     6901 cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac ggtgcccagg
     6961 tcgggctttc tgccacccag ccgccagaga taatggcaaa aataaggaac accaggccaa
     7021 tcactgacac ttcgccaata cgccccggac gcagatagcg caggtagatt cccataaaaa
     7081 tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga gtcagggctt
     7141 tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag caggctacca
     7201 gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc agcgagcgac
     7261 cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg gcgagaacca
     7321 ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc agcaccggcc
     7381 ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc actttcttgt
     7441 ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata cgcgtcgggt
     7501 caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg atcagataga
     7561 tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga tttaaagcaa
     7621 tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg tatttccctg
     7681 atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt tgtttctata
     7741 acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa accaatattt
     7801 aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat ctcatttaat
     7861 tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa cgccgcccct
     7921 gttcatcgaa aacgatgatt tcccagctct gattttgccg cccaagatgc agcggctggc
     7981 agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta agttccgtac
     8041 ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc agcgtttcag
     8101 ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta cgggtatcaa
     8161 ccggcatttc ggcttccagc acatcatctc ccagacgggt atacacaatt cccagatgcg
     8221 ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg cgtttccaga
     8281 tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta gggtgatatg
     8341 gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg gacgggcgat
     8401 tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac gctgttcttc
     8461 ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc caggcgaaac
     8521 cacattacag cgcacgccgc taaccgccag ttccagcccg acgctcaacg ccaggctttt
     8581 cagcgccgct ttcgatgcgc cataagcact cataccaata cgcggcgtgt gtgcggcgtc
     8641 ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca tggtttgctg
     8701 aaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt cctctttact
     8761 gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgaccagca cgtccagtcg
     8821 ctccgtttca gctaacagtc gctgacatac ctgcgcgacc tgcgcagcat cggcaacatc
     8881 catcacttcg gtcgcaaagg gatattgctc ctgagcgaac gcttgatcaa aaccggtaac
     8941 tttcgctccc gcttcaacaa acgccagcgc cgtggcgtag ccgatacctt tacctgcgcc
     9001 ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga gagtagcttc
     9061 caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc gccatgcact
     9121 ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta gtcgatcagg
     9181 ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggataac ctcacgcagc
     9241 gccgctttcc tggcggggac aggtgctggc agtaatcctt cagtcatcac cacccggcca
     9301 gaccgcccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa atcggccagc
     9361 gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc ggtggtcata
     9421 cagccaatgt gggcatatac cccggtaata atcagctggt tacgtccact ctctttgagc
     9481 atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag caccgtgtcg
     9541 tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgaacg ggtcaggccc
     9601 ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt cggctgggcg
     9661 gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc aatattcgcg
     9721 atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata gtcctgcata
     9781 tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt attctgcgga
     9841 atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat cgtgttctct
     9901 ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat cgactttccc
     9961 gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt taaattcggc
    10021 aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct cttttaccac
    10081 cagataagca cagctttttt cgcccatcag ctcatcttcc atgctcacca gtgcggcata
    10141 gatcaccgcc ggatggcgca gcagcaggtt ttcgatctct tcggcagcga tcttctcgcc
    10201 gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct ctggatcaat
    10261 agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg cattgtgctg
    10321 cggacttttg taataaccgc ggaaggtgta cggcccgcgc gtcatcaggc gtccaacttc
    10381 cccttgcggc agtggatttc cttcagcatc agcaacccat acttcatcgt ccggacacat
    10441 tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagtcggg tgtagttcac
    10501 cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag cggaaatacg
    10561 cgccgcaagg gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca gcgaggcaag
    10621 ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg ccggcggcac
    10681 cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg tggcgctggg
    10741 atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc ctggcgaact
    10801 catagcgtag ttatgagccg ccgggatcgc gcacaggtag cgtgtctgtt gtgtgaactg
    10861 acaaatctcg acgctacgac gcacgctgta gtagtagtcg ttatgagtgc gcgggatcag
    10921 cttcggtgta ccggtggtgc cgccggaaag ctggaaatag gccacttcat cagcaggtga
    10981 tggcgtagca gtaaaaccgt ctgccggatg gttaatcgca tcctgcaagt tatgctcacc
    11041 gctgtcgttg agcagttgca ccacgcgaat ggaggaatgt tctgtgacga aagtattgag
    11101 gaaatcatcc ccgctaaaca gcgcatgttg gcgatcggca atcagtaacg ccggttcaat
    11161 ctggctggca taggcgttca gttcactacg ctgatggctg aacaacgcca gcaccggcgc
    11221 aacgcccagt ttcagcagtg cgaaaaaggt aatgtaaagt tcagcgacgt tacccagttg
    11281 taccagcgcg gtttcaccgg gtttaatgcc ctgacggcgt aaactacacg cgaggttatc
    11341 cgccgcctga ttcagctccc ggtaactcaa ctgtcgctcg ccgtcgataa ccgcgatgct
    11401 gtcactcgca gcgtggcgag tcagaatgtc ggtcagcggc aaatcctgcc agtagccttt
    11461 ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc tcatcctcgc
    11521 tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg tttcgcgcca
    11581 ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac gcacctgatt
    11641 ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt cgctgtcaca
    11701 ccaacccaca atgccgccaa acagttcgcg gtcaaacggc tccagttcag caataacctg
    11761 ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca gacaggccag
    11821 agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga gatgccacag
    11881 cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac tgcgttcgcg
    11941 cagtacctct ttcatcgcct gagtcaccag ttcatgttca tggcgatctt tttctgacgc
    12001 cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac gcgcggaacc
    12061 ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt ccgggctggc
    12121 ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa ccgggttttg
    12181 cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg tgatatcaat
    12241 caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg cggcgcgggc
    12301 aaccatctgt tcaaacgtgg tttgttcagg aattgcctgg cgttccacca cgttcagcga
    12361 ctggctgcgg gtaaaacagt gggctgaggt ctgtttttcc tgacgggaga acgactgcca
    12421 ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg ccccgaccat
    12481 caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga gtttttgctg
    12541 gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaagcggg cgaaacatcc
    12601 tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg caagtgttgc
    12661 catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca caaaatgata
    12721 aaggctttaa taatgattat catttatatt ttcggtcgct aacctaaaag caaacgcgca
    12781 ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg ttgtggttac
    12841 aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg tcctgcgccg
    12901 ttttgccccc tctcccttcc agggtgaggg ctggggtgag ggttaatgtt cgcaccagtg
    12961 ctggctgttc ccctcaccct aaccctctcc ccaaaggggc gaggggacgg attgtgcgct
    13021 ttgtcgaatt tgtcattacg cccttaacct tattaataac aggaagctga cttgtgagac
    13081 tcgccccgct ctaccgcaac gcccttctat taacaggact tttgctttca ggaatagccg
    13141 tagttcaggc cgccgactgg ccgcgtcaga ttactgacag ccggggcact cataccctgg
    13201 aaagccagcc gcagcgtatt gtttccacca gcgtcaccct gaccggatca ctgctggcga
    13261 ttgatgctcc ggtgatcgcc agcggcgcga ccacgccgaa taaccgcgtc gcagatgacc
    13321 agggcttttt acgccagtgg agcaaggtgg cgaaagaacg caaactgcaa cggctctata
    13381 tcggcgaacc gagcgccgaa gccgttgccg cgcaaatgcc ggatctgatt ttaattagcg
    13441 caaccggcgg ggattcggtg ctggcgctgt atgatcagct ttccaccatc gccccgacat
    13501 taatcatcaa ttacgacgac aaaagctggc agtcgctgtt aacgcagctt ggcgaaatta
    13561 ccgggcatga gaaacaagcg gcagagcgga ttgcacagtt tgataagcaa ctggcagcgg
    13621 cgaaagagca aatcaaatta ccgccgcagc cggtcactgc cattgtctat accgccgctg
    13681 cacacagtgc caatctctgg acgccggaat cagcacaagg gcagatgctg gaacaactcg
    13741 gctttacgct ggcgaagttg cccgcagggt taaacgccag ccaaagccag ggcaaacgcc
    13801 atgacatcat tcagcttggt ggggaaaatc tggctgctgg gttaaacggt gaatcgctat
    13861 tcctgttcgc cggtgatcag aaagacgccg atgcgattta tgccaatcca ctgctcgcgc
    13921 acctgcctgc agtacaaaac aagcaggttt atacgctggg aaccgagacg ttccgcctgg
    13981 attactacag cgccatgcaa gtgctggaca gacttaattc actgttaaaa aaagattgat
    14041 aaaacataca attagagcac taataatccc ctcgcccctt tggggagagg gttagggtga
    14101 ggggaaaatg cacctttagc taactgtcag acgctgtcac ctgcggcggc gtctggcgaa
    14161 aacgtcgcaa ctccaccagc accagcaata acaacacgcc gataatcaac aaaccaaaac
    14221 cgctcgcgct tgcggaagca accggtgtca tcatcgcgcc caaaccaccc aacagcgccg
    14281 cgcctatggc atcgcccgtc acgttctgcg ccgtccacaa accgttaatc cgccctaaca
    14341 tcgcttccgg ggtttgcgtt tgcagcattg tatattgcag caacgaacta accgcgctca
    14401 gccagccgaa cagcgccaga caaaccacgc ctaaaatcca catcggcatc aggccaaaca
    14461 gaccaatggc gaggaacgat cccagcgtgg agagcagcat caataaccct ggtcgcgcac
    14521 tatgtgccag cttcccgctg gttaacgcac caatagccgc accgagcggg atcgccgcat
    14581 agagaaaacc aatctgtgcc gccgacatct gccagttgtc agccagcgcc ggatacagta
    14641 cccgcaccgc gctcgccatc gtcaataaac cacccagcaa cgcaatccct cctaccagcg
    14701 ggctggcgag cagaaaacga aatcctgcca gtaatgattt caacggatgc tcgcgcggct
    14761 gcggtggcgg tggcaacgcc ggaaggctta acaacggtag caaggtaata aacgtgcccg
    14821 ccgccgccag cccgtagttc caggctacgc cgccggtcgc cagcaataaa ccgccaatca
    14881 tgggcgaaat caccgacccc agacgcacgg tcaacatggt gatcgccccg gcctgcatta
    14941 agttttcacg ccctaccagt gcaggtgttg ccgccagtag cgccgtaaca ccaagtgatg
    15001 cgaaaaaacc atcccataaa ccaagtaaat agattgccag caatgacggc tccggcagca
    15061 gcgcgttcag gcacagtcca atgaagccaa tgccacaggt gccgcgcgcc agcaaaatca
    15121 cttttttgcg ctcatagcga tccgccagca caccgccaac catcaggcca acaaacatcg
    15181 cgccgccggt cagcgtcacc gaaagcccca cctgccaggt agaatgcgtc atcatctgga
    15241 tctgcaccgg caccgcgacg ccgagcaaac ccagagacac aattgagata aaacgagcga
    15301 ggaatactgc gcgaaacgcc gggtgcgttt tcaacaggct gaggttaagc agccaggatt
    15361 gtttattcat tacaatgcct tgccatcaaa tattttaaat acctattctt aggctgcaca
    15421 tgctaacata tccaaataag atcgataacg ataattaatt tcattatcat ggaagttcgt
    15481 atgtctggtt ctgttgccgt gacacgcgcc attgccgtgc ccggattgct gttattactg
    15541 attatcgcga cggcattaag cctgctcatt ggggcaaaat cactccccgc gtcagtagtg
    15601 ctggaggcct tctcaggcac ctgccagagc gccgactgca ccatcgtgct cgacgcgcga
    15661 ctgccgcgca cccttgccgg tttacttgca ggcggtgcgc ttgggcttgc tggggcgtta
    15721 atgcaaaccc tcacccgaaa tccacttgcc gaccccggct tgcttggcgt gaacgccgga
    15781 gccagctttg ccattgtgct gggtgcggcg ctgtttggtt actcatccgc gcaggaacaa
    15841 ctggcgatgg ccttcgccgg ggcgctggtg gcctcattga ttgttgcctt tatcggcagt
    15901 cagggcggcg ggcagttaag tccggtgcgt ttaaccctgg cgggcgtggc gctggcggcg
    15961 gtgctggaag ggctgaccag cggcatcgcc ctgcttaatc ctgacgtcta cgatcaactg
    16021 cgtttctggc aagccggttc gctggatatt cgcaatctac ataccttaaa agtggtgctg
    16081 atcccggttc tgatcgccgg agcaactgcg ctattactga gtcgcgcgct gaacagtttg
    16141 agcctcggca gtgacaccgc gacggcgctg ggcagtcgcg tggcgcgcac acagttgatt
    16201 ggtctgctgg cgattaccgt gctttgtggt agtgcgacgg cggtagttgg cccgattgcc
    16261 tttatcggcc tgatgatgcc gcacatggcg cgctggctgg taggtgccga tcatcgctgg
    16321 tcgctgcccg tcacgctact tgctacccct gccctgctgc tgtttgccga tatcatcggg
    16381 cgggtgattg tgcccggcga actgcgcgtt tctgtggtca gtgcgtttat tggtgcaccg
    16441 gtgctgatct tcctcgtgcg acgtaaaacg cgaggtggtg catgatttac gtctctcgcc
    16501 gattactcat cacctgtttg ctgctgattt ccgcctgtgt ggttgcaggt atctggggat
    16561 tacgcagcgg tgccgtcacg ctggaaatct cgcaggtatt cgccgcgttg atgggcgatg
    16621 cgccgcgcag tatgacgatg gtggtcaccg aatggcgttt accacgcgtg ctgatggcgc
    16681 tgttgattgg cgcggcgctg ggcgtcagcg gcgcgatttt tcagtcgctg atgcgtaacc
    16741 cgctcggcag ccctgacgta atgggcttta acaccggggc gtggagcggc gtgctggtgg
    16801 cgatggtgct gtttggtcag gacctgacgg ctatcgcgct ggcagcaatg gtgggcggca
    16861 ttgtcacttc tctgttggtc tggctgctcg cctggcgtaa cggtatcgac acctttcggt
    16921 tgattattat cggcatcggc gttcgcgcca tgctggtggc ctttaatact tggctgttgc
    16981 tgaaagcgtc tttagaaacg gcgctaacag cgggtttgtg gaatgccgga tcgctcaatg
    17041 gcctgacgtg ggcaaaaact tcgccttccg cacccatcat catattgatg ctcattgccg
    17101 ccgccttgct ggtgcggcga atgcgcttgc tggagatggg cgatgatacc gcctgcgcgc
    17161 tgggcgtcag cgtcgagcgt tcgcgtctgt taatgatgct ggttgcagtg gtgcttaccg
    17221 ctgcggcaac ggcgcttgcc gggccgattt cctttattgc tttagtcgcg ccgcacattg
    17281 cccgacgcat tagcggcacc gctcgctggg ggctgaccca ggcggcgctg tgcggagcgc
    17341 tgttactgct ggcggccgat ctctgcgccc aacaactgtt tatgccgtat caacttccgg
    17401 ttggcgtcgt taccgtcagc ctcggcggta tttaccttat cgtcttgtta attcaggagt
    17461 ctcgcaaaaa atgaccgaat cagtagcccg tttgcgcggc gaacagttaa ccctgggata
    17521 tggcaaatat accgttgcgg aaaatctgac tgtagaaatt cctgatggtc acttcacggc
    17581 aattatcgga ccaaatggct gtggtaaatc cacgttactg cgtaccttaa gccgcctgat
    17641 gacgcctgct cacgggcatg tctggctgga tggcgagcac attcaacatt acgccagtaa
    17701 agaagttgca cgccggattg gtctgttggc gcaaaatgct accacgccgg gcgatatcac
    17761 cgtgcaggag ctggtggcgc gtggacgtta tccgcatcaa ccgctgttta cccgctggcg
    17821 caaagaggat gaagaagcgg taacgaaagc gatgcaggcc acgagaataa ctcatctggc
    17881 agatcaaagc gtggataccc tttctggcgg acaacgccag cgagcgtgga tcgcgatggt
    17941 gctagcccag gaaacggcaa ttatgctgct cgacgaaccc acgacctggc tggatatcag
    18001 tcatcagatt gatctactgg aactgttaag cgaactgaac cgcgagaaag gctataccct
    18061 ggcggcggtg ctgcacgatc ttaatcaggc ctgtcgttac gccagccatt tgattgcatt
    18121 gcgggaaggg aaaattgttg ctcagggagc gccgaaggag attgtcactg ctgaactgat
    18181 tgagcgcatt tatggtctgc gatgcatgat cattgacgat ccggtagccg gaacgccgct
    18241 tgtggtgccg ctcggacgaa cggcaccttc aaccgcaaat agttaaacta agtggtctgc
    18301 catcatggca tcctgttttc tggatgccat cgcatagcgc aataacacgc taccacaagc
    18361 caccatcccg ccgatcagcg cggaaaggat cacaataatc gccttacccg gaccgtcttt
    18421 tttcactggc aatgacgggc ttaactgata tttaaacggc gtaaaattca catcgttgac
    18481 atttgctttt gttaattgct cgacaagata ctgccgatta cgtaattcac cgttcagttc
    18541 cgcaacgtca gtaaccgctt tttcaatttc cagtttgcgt tcaataccgt ctgcgccgag
    18601 agaaatagag aaatcggggt catctttaac ggcctgacca ttactgtata cgggcttttt
    18661 aattcctgcc gcgttggcaa tgtcgagtga ataattgagg cgctgaatgt ttgcatcaag
    18721 ttgatttttc gttttaatgc gatcctgagc cagtttttct ttttcaaact gggttttgat
    18781 ctccagttta ttacggacgt tttctatcga ctctttcacc accaacgcag agatataatc
    18841 gatatacccg ctcaaaacgg tctgcgcctc tttactggtt ggcgcggtaa aacttagcgt
    18901 ccaggaggta tacagtgacg gttcatcttt tttcttactg gcattgtcat caaccgcttt
    18961 cattttttcg ctcaatgcaa caatagcgcg atgcaaatcc agttcatcga ttttcgcctc
    19021 ttttaattgg tccatcacat aaggtgatga acgcaggtac tcttccagca agctaacaga
    19081 ctgaaacttc ttgataaaca ggttaaatgc accgccacgg tcgatattaa catcgagatc
    19141 aagaacgcgg agtttggtaa aggttttctc caggtcctgc cactgaatgg cttcggcggg
    19201 agtaataacg gctgagctgg tccatttttg cggcaggatg aaagagatca gtaaacctgc
    19261 gcaggcaaat gcaaaaacga ccgccatgac cgtttttttg gcccgccata aaacttcgat
    19321 tagattaagt aaatcaattt cattattact gggcgacgcc agaggatact cagaaaaatg
    19381 agcgtcactt ccctgtttaa tattcaatga tgacatgggg tataacctga aaatgagtcc
    19441 aataggggaa aatctccacg gcgctcactt taccagttgg tgagaattat ccgaagctga
    19501 agtttgtaaa ttccttccac tgaatgcaat attcactcta tttaagagaa atattcatca
    19561 aacctataaa tcaaacaaat aaaaaaccca taattatttt ctgtaattat gggtttatag
    19621 ataatattaa tttacctgtt tagcgttgcg cgaataatcg gcccaatttt tacaaacgcc
    19681 cctggagaga taatatccac atgcgcacaa tcctgacgat agatatccag ctccgcaatc
    19741 cacggcgacc aggcgcgttc aggactcata ccttcctgaa gcgtgcgttc agcaacaaac
    19801 aacgtcgctt taccgtcaaa cggtacgcta tgagcagtcg tcagcaggcg cacagcatca
    19861 gcgtagttgc cttcaatggt ggtaaacaac tccgttgaag tacttccctg ctgtgccgcc
    19921 aggaaggcct cgcgctcgcg gttaatctcc gccagcactt ccgggtccag accattagct
    19981 tctttttcct gccagttttg cgtttctggc ggccaggtat ccagcaagcc aagaaatgcc
    20041 acctgttcgc cacgggcacg cagtcgcgcc gcaataccct gcgccagcgt accgccaagg
    20101 gaatacccca gcaggtaata agggccgtgc ggttgttgct caagtaacgt tgccagatgc
    20161 gcttcgcaga cttcatccag gtttgtcgct gtctgcatgg gtccatgagg gcgcggcgac
    20221 tgaatgccaa taatcgacca ttgtggatcg agataacgcg agagtacgct aaactgccag
    20281 gcaaaaccgg acgccggatg gaaacaaaac agcgtcgggc cattaccttc acgcaacggc
    20341 agaatggttt cgaatcccat gcgccgggag ctgtcctctt caccatcaat aatcgttgcc
    20401 agtttggcga cggttgacgc gaccatcacc tgccccggcg tcacctgacg agcaaactgt
    20461 cgacttaact gcgctgccag tttcattgcc agtagcgaat gaccgcccag cgcaaagaaa
    20521 tcagcatcgg catcctgcac gtcacaaccc agcaacgacg cgaatgccgc ggcgataatc
    20581 gtttcactgc ccgctttcgg tgcacgtccc gacgcttgtg tcttcagttc aggcaacggt
    20641 aaggctttgc gatccagctt gccgttggcg ctaagaggta actgtggaag ttgcagcaga
    20701 accaccggta ccatatgtgg tggcaatgtt tcacgaagct gcgcctgcaa tgcgctggta
    20761 tccaacggca ggcccgattg cgacaccaga tagcccacca actgacgcgc atcaccacca
    20821 gtggctgccg cctggttaat cacacaggcg tgggtaacgg cttgttcgac atccggcagc
    20881 gcctgcatca cgcgatcgat ttcgcccagt tcgatacgct gcccgcgaat ttttagctga
    20941 tcgtcactgc gcccgaggta ctccaccgcg ccgttatcca gccagcgggc aacgtctccg
    21001 gtacggtaca tccgttcgcc aggagcaaaa ggatcggcaa taaagcggct ggcggtcaga
    21061 tcggggcgtc cgagatagcc ctgcgccagt tgaatgccgg taagatagag atcacccgcc
    21121 acacccggcg gcaccggatg catcatcgca tcaagaatac gcagccccat gttccacacc
    21181 ggataaccaa tcggcacact gctgccgcgc acctgtgcca gttcctcgcc aaaagccgga
    21241 taccagctga catctaccgc cgcttccgtc gggccataca gattatgtaa cggcgcaccg
    21301 gttaactgtt gccattcacg gcataaatca gccggtaagg cttcaccact acagaaaacc
    21361 tgtttcaacg tcgcgcaact ctggcgagcg gtttgcggag tcagcgaggc aacaaatgcc
    21421 gccagcatcg acggcacaaa gtgcgtggtc gttacgccat attcggcaaa gaattgctgc
    21481 atagcgagcg ggtcacggtg cgcttccggt tcagccatca ccagttttgc ccctgcgata
    21541 aacggccaga aaaactccca caccgagaca tcaaaactgc acggcgtttt ttgggcaacg
    21601 acatcttcgc ctgtaagcgg gtagtgattt tgcatccata acaggcggtt aacgatagcc
    21661 gtctgcccga ccattacccc tttcggcctg ccggtggagc cagaggtaaa gatgatataa
    21721 gccgtgtggt gcggttgtga aagttgcagc ggcgcactgc cctggggggt aagcggggcg
    21781 ttatagcaaa ggcttgttaa attgggaaca tcgctaaagc gcggcagttg atcgtcggtg
    21841 gtaattaaca gcgacggacg cgcatcttcc agcatcattt tcaggcgatc gtccggatag
    21901 ccggtatcca gcggtagcca ggccgcgcca gcttcaacta ttgcatggag tgccagggtc
    21961 aaaaagaccg agcgcggtag tgccaccgcc acgctgtccc ctggtttaac gccgcgctca
    22021 cgcagcagat tcgccagcgc caccacctgc tcgcgcattt cccgatagct gaacaggtaa
    22081 cgcgcatctg ccagcgccgg agcatccggt gtttttgccg cttgttctgc caccagcgcg
    22141 ctcagcgtgg tttctggaat ctcaacctga gtggcgttga tctgcgccag ctgcgcatac
    22201 tcacctggca gcataatatc gacatcgccg cacaacagcg ccggatccgc ggcgaactgg
    22261 gcaatcagca ttttcaggcg ttcagcatgc tggattaacg ttggctcatc gtaacgctgt
    22321 ttattggcga ggatctcaat actcaaatca ccgtgttcat ccgggaacag ggccagttca
    22381 aggtcattaa ccggaccggt tgccagggta tgggtttgcg cctgaacacc aggaatatcc
    22441 agttggtaat caaatacctt aatattgagt accggaccaa acagcggttc atcacctgcc
    22501 gctcgcccgc tgtcacggac aatttgttcg gcatcgtaac gttgatggcg acgcattttt
    22561 ttcagttgag ccgccagtcg ggttgccagt tccggcagcg tttcttgcgc cgcaatgtga
    22621 atacccaacg gtaaaacgtt gagcacgggt ccgctagccg tcagcgccgc cgagcccagt
    22681 cgacgcataa agataaatcc ggcggcatag tccatgcgat tgcacaatcg ccccagccac
    22741 aaggctgcca gcgcaagggc taaatcggta cgctgcacac ctgaaagttg cgtagccagc
    22801 tggcggaatt ccccgtcggt aaattccagt ttcaggcgca gaatatctgc cgaagcgctg
    22861 cgccccggta aaggtgccgg agaaagtgac gcgggcggcg gcagttgacg acgctgttct
    22921 gcccagaatg ccgcatcacg ctgccaggct tcgctttcgc ggtattgctg gtactcttcc
    22981 actacatcag cgaaaggcgt aaacggcgaa gcaggcgttg gttcgccacg cagccatgtg
    23041 cagtaaatat tggcgatctg gcgagtaatg gccgggaaac tgaagccatc gaccagcaaa
    23101 tggtgataac gctgatacca gtaccagcgg ttatccgcaa catgtatcag ctgatgaaag
    23161 accagtggtt taccgctatc gacgcgcaaa tcttgttgca aatccgtcag cattaatgcc
    23221 tgcgcagtac cgtgcggatc aatattggtt cgcaggtcga taatttctgg ctgttcgaat
    23281 atcagcgcat catcgaccca ctgccagact tcgccgttat cttccgtaaa acgcatccgc
    23341 aacgtatctg cttgcgctag tccggcaacc accgcgcggg ccagtaatgg cgcatcaaca
    23401 tctccagtta actcaacgta atgcgccacg ctccaggcgg agggtaaatc cgacagtttt
    23461 tctgccatcc agatgccggg ctgtgcggcg accaaaggta aatgctggct cattgtgcct
    23521 cctgcaactg ggtgaaattg gccggtgtca gagtacgcca gtggacttcc agccactgct
    23581 ggcaggacgc ctgtgactgc ggctgacaca caatgtccca gcctgccgat aagaggcatt
    23641 gttgcggcca cagactgaat tgcccctgcg cattgcgcaa aatgtaaaac gctccctgcg
    23701 gatcatcgaa gggattactg aatgccatac tcaactcctg tcatggaaaa gtggttgcca
    23761 gaggtcgatt aacccctgca tcaagccacc gcgccaacaa agcgcatcat gtccgccgtc
    23821 aacctgacgc cagaaaatgg attcttttat agggtgcaat tgcgcataca gcgcctgatt
    23881 ggcccgcatg atcatcggct cgcgaatacc cgcttccagc acaatgcgca gaccttcggc
    23941 gctaacttca ccagctttta gcttttcaag taacacgccc tcttgctgcc cgtcccgatg
    24001 cggccaccag tacgatcctg actggcttaa tacacagcca aagcgttcag gccagtgcag
    24061 tccggcatac agcgcggaca gcccaccaaa actctgcccg gcgaccacgg tgcgatcggc
    24121 gcgatcgcta aaaggggcaa tagctttcac caggggtaat aactcttgct gtactgccag
    24181 ccagaaatcc gcattacacg gcagttcgtg ggcgcggtgc gtggtgtcga tagcgtcgat
    24241 caacacatac acggcgggag gaagttgctg acgatgggtc agcgaagtca gcacaggcca
    24301 gacgggcata ctttgcgccc aaaattcgcc atcgagcaaa actgccagcg gacgttcttc
    24361 tgctgttaca tcgccggtgg taaaaatcca tacacgacgt gaatttttca accgttcact
    24421 tttccagata atttctttgg ctgctatttc tggcacttgc ggacaatccc atcccggttg
    24481 cagaggcgct tgcggcattt cgagtgcaga aacagcgtgc cctcgcccgc ctttccagct
    24541 ttgtggattc agcggatcgg ctattgcctg gggtaatagt tttcgccagc cttcacgtaa
    24601 ttcgaggcga tcgggggatg gtgcagaaaa aatgtcatcg cgttcggtgg gaataaagca
    24661 gtagctgccg cgccagttgg cattgagttg tgtcgtccac tgccagacat cagtacctgc
    24721 aattcgctgc atcgactggg gctggctgtt ctgatgatga tcggtcacac cagtgatgta
    24781 gatccatacg cgctttatcg tcgagtattc ttcagaacct tggggatcac gccaccagaa
    24841 agtgacctca aacatttcgt cattcagacg ctgccattcc gggccatgtt tcgactgcca
    24901 ccagctctca cttcctactt ttaacgccgt cacaccataa ccccatgttt actgtgcaat
    24961 ttttcattga ttgcagaaat atattgataa tattattgat aaccatttgc atttgcaata
    25021 gcgtaatggc gcgccgtggg aagcgcggac atttattaac caactgcact gcgtgtcttt
    25081 caggatcaaa ggttttcgcg gtagcgggat gcgtcgtgtt gaggacgacc atgcccgaca
    25141 gttgcaattc gtggcaaaaa tgcaggaata aaacaatgaa caagaagatt cattccctgg
    25201 ccttgttggt caatctgggg atttatgggg tagcgcaggc acaagagccg accgatactc
    25261 ctgtttcaca tgacgatacc attgtcgtta ccgccgccga gcagaactta caggcgcctg
    25321 gcgtttcgac catcaccgca gatgaaatcc gcaaaaaccc cgttgcccgc gatgtgtcgg
    25381 agatcatccg taccatgcca ggcgttaacc tgaccggtaa ctccaccagt ggtcagcgtg
    25441 ggaataaccg acagattgat attcgcggta tgggtccgga aaacacgctg attttgattg
    25501 acggcaagcc ggtaagcagc cgtaactcgg tgcgtcaggg ctggcgtggc gagcgcgata
    25561 cccgtggtga tacctcctgg gtgccacctg aaatgattga acgtattgaa gttctgcgtg
    25621 gtccggcagc tgcgcgttat ggcaacggcg cggcgggcgg cgtggttaac atcattacca
    25681 aaaaaggcag cggtgagtgg cacggctcct gggacgctta tttcaatgca ccagaacata
    25741 aagaggaagg tgccactaaa cgcaccaact tcagcctgac cggtccgctg ggcgacgaat
    25801 tcagcttccg cttgtatggc aacctcgaca aaacccaggc tgacgcgtgg gatatcaacc
    25861 agggccatca gtccgcgcgt gccggaacgt atgccacgac gttaccagcc gggcgcgaag
    25921 gggtgatcaa caaagatatt aatggcgtgg tgcgctggga tttcgcgcca ttgcaatcgc
    25981 tggaactgga agcaggttac agccgccagg gcaacctgta tgcgggcgac acccagaaca
    26041 ccaactccga ttcctatacc cgctcgaaat atggcgatga aaccaaccgt ctgtatcgcc
    26101 agaactactc gctgacctgg aacggcggct gggataacgg agtgaccacc agcaactggg
    26161 tgcagtatga acacacccgt aactcgcgta ttccggaagg tctggcaggc ggtaccgaag
    26221 ggaaatttaa cgaaaaagcg acacaggatt tcgtcgatat cgatcttgat gacgtgatgc
    26281 tgcacagcga agttaacctg ccgattgatt tcctcgttaa ccaaacgctg acgctgggta
    26341 cggagtggaa tcagcaacgg atgaaggact tgagttccaa tacccaggcg ctgaccgggg
    26401 cgaacaccgg cggcgctatt gatggtgtga gtgccaccga ccgtagcccg tattcaaaag
    26461 cagaaatttt ctcgctgttt gccgaaaaca acatggagct gactgacagc accatcgtaa
    26521 cgccggggct gcgtttcgat catcacagta ttgtcggcaa taactggagc ccggcgctga
    26581 acatatcgca aggtttaggc gatgacttca cgctcaaaat gggcattgcc cgtgcttata
    26641 aagcaccgag cctgtaccag actaacccga actacattct ctacagtaaa ggtcagggct
    26701 gctatgccag cgcgggcggc tgctatcagc aaggtaacga tgacctgaaa gcagaaacca
    26761 gcatcaacaa agagattggt ctggagttca aacgcgacgg ctggctggcg ggcgtcacct
    26821 ggttccgtaa cgattatcgc aataagattg aagcgggcta tgtggctgta gggcaaaacg
    26881 cagtcggcac cgatctctat cagtgggata acgtgccgaa agcagtggtt gaaggtctgg
    26941 aaggatcgtt aaacgtacca gttagcgaaa tggtgatgtg gaccaataac atcacttata
    27001 tgctgaagag tgaaaacaaa accacgggcg accgtttgtc gatcatcccg gagtacacgt
    27061 tgaactcaac gctgagctgg caggcgcggg aagatttgtc gatgcaaacg accttcacct
    27121 ggtacggtaa gcagcagccg aaaaaataca actacaaagg tcagccagcg gttggcccgg
    27181 aaaccaaaga aatcagtcct tacagcattg tgggtctgag cgcgacctgg gatgtgacga
    27241 agaatgtcag tctgaccggc ggcgtggaca acctgttcga caaacgtttg tggcgtgcgg
    27301 gtaatgccca gaccacgggc gatctggcag gggccaacta tatcgcgggt gccggggcgt
    27361 atacctataa cgagccggga cgtacgtggt acatgagcgt aaacactcac ttctgatgct
    27421 aacgtcagat tgttgacaaa gtgcgcgtcg ttcatgccgg atgcggcgtg aacgccttat
    27481 ccggcctaca aaaatcttgc caattcaata tattgcagga ccatgtaggc ctgataagcg
    27541 tagcgcatca ggcagttttg cgtttgtcat cagtctcgaa tatggtcgat atgaaaacta
    27601 cgcatacctc cctccccttt gccggacata cgctgcattt tgttgagttc gatccggcga
    27661 atttttgtga gcaggattta ctctggctgc cgcactacgc acaattgcaa cacgctggac
    27721 gtaaacgtaa aacagagcat ttagccggac ggatcgctgc tgtttatgcc ttacgggaat
    27781 atggctataa atgtgtgccc gcaatcggcg agctacgcca acctgtctgg cctgcggagg
    27841 tatacggcag tattagccac tgtgggacta cggcattagc cgtggtatct cgtcaaccga
    27901 ttggcattga tatagaagaa attttttctg tacaaaccgc aagagaattg acagacaata
    27961 ttattacacc agcagaacac gagcgactcg cggactgtgg tttagccttt tctctggcgc
    28021 tgacactggc attttccgcc aaagagagcg catttaaggc atcggagaga caaactgaag
    28081 caggttttct ggattatcag ataattagct ggaataaaca gcaggtcatc attcatcgtg
    28141 agaatgagat gtttgctgtg cactggcaga taaaagaaaa aatagtcata actctgtgcc
    28201 aacacgatta attgacaaca tctggtacga ttcgcccgca gccatcactg actgcgggcg
    28261 aaagtgtaaa gcaggtgcct taccactctg acctgacaac cggatatgca gggattgctc
    28321 cccgcacaac ggctacttct tcggttcgta agcgagaaca gccttaaact caatattacg
    28381 ttcctttacg gtgaactcac acagcgagtc tccgaccaga agcgtaaatc ccagcaccgt
    28441 caaacacagc actatgactg ccacaagggc atatttcgtc agcatgttta tattgcctcc
    28501 atgcgaagaa gggactacca tccgagttgt tgaggttcgg agtggcagcc ccaggattga
    28561 tagaaatatc tcctggggct tcatctttcc agacctcaga gtaaacctga aaccggaaag
    28621 cttcaggcac ccgccgttat cttacccttc ctcagcaaaa tatctgttta tttatacagt
    28681 aagtcctgtt tcggacttct gttggcaaca ctcgttattg ctcgtaaaac gaaatcgccc
    28741 gcagccatca ctgaccgcgg gcgaaagtgt aaagcaggtg ccctaccatc ctgacctgac
    28801 aaccggatat gcggggatta ctccccgcac aacggctact tcttcggttc gtaagcgaga
    28861 acagccttaa actcaatatt acgttccttc accgtaaact cacacagcga gtctccgacc
    28921 agaagcgtaa atcccagcac cgtcaaacac agcactatga ctgccgcaag ggcatatttc
    28981 gtcagcatgt ttatattgcc tccatgcgaa gaagggacta ccatccaaat tgttcgggtt
    29041 tggagtggca gccccagggt tgatagaaat atctcctggg gcttcatctt tccagacctc
    29101 agagtaaacc tga
//
LOCUS       DAZOZY010000031        28894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZOZY010000031 DAZOZY010000000
VERSION     DAZOZY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.031"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHURQ_001200"
     CDS             complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHURQ_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR0823033.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
     gene            1753..2310
                     /gene="pinE"
                     /locus_tag="ACHURQ_001201"
                     /pseudo
     CDS             1753..2310
                     /gene="pinE"
                     /locus_tag="ACHURQ_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2649..3497)
                     /locus_tag="ACHURQ_001202"
     CDS             complement(2649..3497)
                     /locus_tag="ACHURQ_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR0823034.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHURQ_001203"
     CDS             3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHURQ_001203"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR0823035.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4399..4902
                     /gene="tadA"
                     /locus_tag="ACHURQ_001204"
     CDS             4399..4902
                     /gene="tadA"
                     /locus_tag="ACHURQ_001204"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR0823036.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHURQ_001205"
     CDS             complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHURQ_001205"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR0823037.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6713..10600
                     /gene="purL"
                     /locus_tag="ACHURQ_001206"
                     /gene_synonym="purI"
     CDS             6713..10600
                     /gene="purL"
                     /locus_tag="ACHURQ_001206"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR0823038.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11158..12585
                     /gene="qseE"
                     /locus_tag="ACHURQ_001207"
     CDS             11158..12585
                     /gene="qseE"
                     /locus_tag="ACHURQ_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR0823039.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12750..13463
                     /gene="qseG"
                     /locus_tag="ACHURQ_001208"
     CDS             12750..13463
                     /gene="qseG"
                     /locus_tag="ACHURQ_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR0823040.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13453..14787
                     /gene="glrR"
                     /locus_tag="ACHURQ_001209"
     CDS             13453..14787
                     /gene="glrR"
                     /locus_tag="ACHURQ_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR0823041.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14848..15186
                     /gene="glnB"
                     /locus_tag="ACHURQ_001210"
     CDS             14848..15186
                     /gene="glnB"
                     /locus_tag="ACHURQ_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR0823042.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHURQ_001211"
     CDS             complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHURQ_001211"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR0823043.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16749..18002
                     /gene="glyA"
                     /locus_tag="ACHURQ_001212"
     CDS             16749..18002
                     /gene="glyA"
                     /locus_tag="ACHURQ_001212"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR0823044.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18200..19393)
                     /locus_tag="ACHURQ_001213"
                     /pseudo
     CDS             complement(18200..19393)
                     /locus_tag="ACHURQ_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19511..22788
                     /locus_tag="ACHURQ_001214"
                     /pseudo
     CDS             19511..22788
                     /locus_tag="ACHURQ_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22885..23868
                     /locus_tag="ACHURQ_001215"
     CDS             22885..23868
                     /locus_tag="ACHURQ_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR0823045.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23891..25402
                     /locus_tag="ACHURQ_001216"
     CDS             23891..25402
                     /locus_tag="ACHURQ_001216"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR0823046.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25427..26425
                     /locus_tag="ACHURQ_001217"
     CDS             25427..26425
                     /locus_tag="ACHURQ_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0823047.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26458..27552
                     /locus_tag="ACHURQ_001218"
     CDS             26458..27552
                     /locus_tag="ACHURQ_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR0823048.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27564..28436
                     /locus_tag="ACHURQ_001219"
     CDS             27564..28436
                     /locus_tag="ACHURQ_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0823049.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28484..>28894)
                     /locus_tag="ACHURQ_001220"
     CDS             complement(28484..>28894)
                     /locus_tag="ACHURQ_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0823050.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6866 a         7143 c         7864 g         7021 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gtgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggagaatg cccgggaatt ctcctcggtg
      241 gaaaaagaag tccattgtgt agaaacctcc cgacgatctg cttcaggaaa ccaggctgcc
      301 acagtttcgg gaagaggggg gatctgaaag ttctgccccc cttcagtgat gactctgata
      361 tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa acagtctgtt ttggtctgac
      421 agggaaatac gaaactgagg cccgttaaaa tttggaaccg aagtttgctg tattaagaat
      481 agcagagttt gatctgataa ggggttattt gcaatctcaa tagttatatg aggatctaaa
      541 cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt caaaataaaa aaatcttctc
      601 atgataatat tttcttggat gtttggtaag ttgcttaatc tattgctata tgcaaaaaga
      661 agttgcagtc tcctaggtaa agggggcagt tcagtaagat ggttgtgcgc aaccttaagt
      721 atcttaagat tagaaggcag agatggaaga gtttgcagtt cgttgttcct aacctcaagt
      781 ttttttaaat tcaagggtaa tgaaggcagt gtggataacc tattatactc tgcgcttagt
      841 gatttgatgg tgtctggtaa taacggcaga ttctctaatt gattttcgga cacatccaaa
      901 gttattagat aatgtggtaa tgcagatata tgtgttaaga aattatctct tgcaataagt
      961 tctttaatat tttcaggtaa atcagtaagg atggttaatt tattacagct cacatcaagc
     1021 tttttaatgc aagcagggag tacaggtaac gaggaaagcg ccaaggctcg caggtccagt
     1081 ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt acgctgttct
     1141 tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt gggggatgta
     1201 attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt aatttctcgc
     1261 atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc ctaatgagga
     1321 gggtgattca gaatattcgc cataagggaa tgagtgttat atcatgcttt ctaccctggt
     1381 aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt acatattcac
     1441 aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca acatctccaa
     1501 ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa tcatgtataa
     1561 aataagctta tatacatagt aacttcagca aaggatttag taatgatcca aatcatatct
     1621 caatgtactg tttttttctt taaaaaaaca gtacattcta ctttataatg actgtgtatt
     1681 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
     1741 gggggattta ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
     1801 ctacaacgta atgcgttgaa ctgtgcagga tgcgagctga tttttgaaga caagataagt
     1861 ggcacaaagt ccgaaaggcc ggggctgaaa aaactgctca ggacattatc ggcaggcgac
     1921 acactggttg tctggaagct ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg
     1981 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgactgattc aattgatacc
     2041 agcacaccaa tgggacgctt tttcttccat gtgatgggtg ccctggctga aatggaacgt
     2101 gaactgattg ttgaacgaac aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt
     2161 ggcggtcgcc gtcccaaact ttcaccagaa taatgggcgc aggccgggcg actaattgca
     2221 tcaggagttt ctcgccagaa ggtggcgatc atctatgatg ttggcgaatc gacttttttg
     2281 tataagaggt ttccagccag ggttatataa aaccgcagac acggcgtata caagaacgtg
     2341 ctacggttga ctgaaaagtt aatgtggtat gtcacaggcc ttttgcgccg taaagccccg
     2401 tcctttaggg cgcggaggac gttaaagaaa gtggtgttct ttgtgttgct gaacttatgc
     2461 aaaatttttt tgcaccataa atccaacaac acattcaata acacattgct gtatcactat
     2521 gaaaataaat gtgtttttat ttgatttcac acatatcaca attgatgcat tttttagtga
     2581 ttaacaacgc cataaggaaa ctctcaaaac aacacaaacc gggcgggcat tgtacgcgca
     2641 ttctttactc agaccagttt tttcaccagc gcttcactat gacgaatacg ttctggtgcc
     2701 agttccagat cctgctgaat cagcgcaatg aacagcaaat ccgttaacat tccctgagcg
     2761 tgacaagcag agattgaagc actgtttgtc gcttgttctt cggcaatggt atacaggcaa
     2821 tgagaagcac gctgttgcag ggcattcgga gtaaagccgg taatcgccag cacttttccg
     2881 cccactcgca gcatctcatc tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa
     2941 atggctaata acagatcgtc aggggacgac gcctgtactg ttgcgagtag cgcatgcata
     3001 tcgcgcactg cggcagcatt gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc
     3061 accagacccg aagcgccaat accggtcaga attatccgcc gcgcagagcg caacattgtt
     3121 acgcattcat gcagtttctc ttcactatta acgtttagcg ttgcgtacat agcggcagta
     3181 ttttctttaa tcagtttttc gccgaccagc cgtaacggat catcaccgcg gatttggtta
     3241 tgaatgggca cggagggcga ttccggctgg cttgccagcg cttcactcaa cgccaactta
     3301 agcgccggaa aacctttata gccgagtttt tgcgcgaact tcacgacgct ggactgactg
     3361 actccggctt cgttggccag ttgctgagag cttaaatggc gcgccgtatc aggttgtagc
     3421 agcagataat ccgccagttt tttatcactc tgggcaagcc cctggtaacg ctgacggata
     3481 cgaagtaagc cgttcatatc tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga
     3541 gacacacgcg agtgaatttt atattccatt gccgctaatt gccatgaatt aaaaattcca
     3601 gttagccatg atgcggtcga ttgttgccgg aaaagtgatg atctatctgt taggctatgt
     3661 agtagcgaaa attaatcagg atgtttcagt ccagaggagt atggtttggc aactcacgag
     3721 cgtcgtgtgg tgttttttga cttagatgga acattgcatc agcaggatat gttcggcagt
     3781 tttctgcgct atttactgcg tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta
     3841 ccgattatag ccattgcgtt attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg
     3901 cttctgtggg ggtgcacttt tggtcacagc gaagcacgtt tacagacgtt gcaggccgat
     3961 ttcgtgcgct ggtttcgcga caatgttacc gcctttccgc tggttcagga gcgattaacc
     4021 acctacctgt taagttccga tgctgatatc tggttgatta ccggctctcc gcagccgctg
     4081 gttgaagcgg tttatttcga tacgccctgg ctgccgcggg ttaatcttat cgccagccaa
     4141 attcagcgtg gctatggtgg ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc
     4201 gcacaactgg agcgcaaaat cggcactccg ctgcggctgt acagtggcta tagcgacagt
     4261 aatcaggaca atccgctgct ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt
     4321 gaactccagc aactggaata gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt
     4381 ataaccggag ttttcttttt gtctgaagtc gaatttagcc acgaatactg gatgcgtcac
     4441 gcgctgacgc tggcgaaacg tgcctgggat gagcgggaag tgccggtcgg cgcggtatta
     4501 gtgcataaca atcgggtaat cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc
     4561 accgcacatg cagaaatcat ggccctgcgg cagggtggtc tggtgatgca aaattatcgt
     4621 ctgatcgacg ccacgttgta tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg
     4681 atccacagtc gcattggtcg cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg
     4741 ggatctttaa tggatgtgct gcatcatccg ggtatgaatc accgagtgga aattacggaa
     4801 ggaatactgg cggatgagtg cgcggcgttg ctcagtgact tctttcgcat gcgccgccag
     4861 gaaattaaag cgcagaaaaa agcgcaaccc tcgacggatt aattttgttt ctcttcactc
     4921 cctttcctgg aaaacagcag agagggagaa tgggtcaaat agtttgatga cgactgggaa
     4981 agaaacgaga gaaaaggaaa tttctctttg cccaactccg taggcgatac cgccggataa
     5041 tcctgcgcca gttgcatcgc cgcttctgta gcctgcttct ctttctcttg cagataaccc
     5101 accaggctaa tctgatactt acgaatattt tcgacataag cgtaggcttc atgtccacga
     5161 gcgtagccgt aagtcagctt gctgtaatag ggtttctggc taagtaaagg cagacgctgt
     5221 tttacgtcag cccaactgtc aggattccct ttggtttttg ccgtcagggc gcgggcatcc
     5281 agcatatgcg catagcccat attgtacgca gcgagggcaa accagatccg ctcgttctcc
     5341 ggcacacttt ccggcacttt actcatcata tcctgcaaat aacgcacgcc accgctgatg
     5401 ctctgttcgg catcggtacg atccgtaatg ccgaggcttt gcgcggtatt tttggttaac
     5461 atcatcatgc cgcgcacacc cgtcggtgaa gtggcctgtg catcccagtg cgattcctga
     5521 taagcaatag cggccagcaa acgccagtca atttcttcgg cgtatttctc aaacaggggc
     5581 tttaactgcg gcagtaccgc atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca
     5641 aaatcatcgc catgccccag gtatttctct tcaatgcgtg ccagcgtaca gtcttcattc
     5701 atttcgttga agaagtcgag cagggcggcg gaaagggtat tatcgccatc taacgggcta
     5761 aaccaagtca ccggttgttc atcggtgata tcgagcgcta cggcgagctc cgggtgaacg
     5821 cgctgaaaca ggctgatggc gacagaatca gcaatggtgt aatcgagttt tccttcgatg
     5881 acatcttcca ttaattccgc agagcctttt ttgtcgtcta ccttccagct taattccggg
     5941 aattttgtgt ctttcagggt ttggagatcg ttaaccacca cgtgacccgg tgcaacggtg
     6001 agttgctccg ccgtcaggtt gcccagtgtg cgcgggcgat actgactcac tttataaacc
     6061 agttgttgtg acacggaata ataggtaggg ccaggctgat aattttttac ccgctcactg
     6121 ttatagacca gtcctgccgc cagcaggtcg gcgttaccat tatcgaggtc gtcaaacagc
     6181 tggctgatat tctgccgcac ggtcactttc agttttacac cgaggtaatc ggcaaactgt
     6241 ttcgccagtt cgtaatccag gccaaaaggt ttcccgttga tttcgttata agtcagggga
     6301 gtatgaatgg tgctcacacg caactctccc cgcgcttgaa tggcggcgat acggttgtcg
     6361 gctttaccaa accagggaat ggatggccag agagcgaccg cgagcagcag tgccagaatg
     6421 ccgatgaaca gataattaat ctttaatttt ttcaattagt taattctctg tgtcgtgcgc
     6481 gtcccagctt gaaaaaacgt aataatagtg aaaggtttac tcataaatga gcggcatttt
     6541 gcgtaaacct gcgccagatg gcaacttatt acagccattg gcggcacgcg ttgctaattc
     6601 acgatggtga ttttatttcc acgcaaacgg tttcgtcagc gcatcagatt ctttataatg
     6661 acgcccgttt cccccccttg ggtacaccga aagcttagaa gacgagagac ttatgatgga
     6721 aattctgcgt ggttcgcctg cactgtcggc attccgaatc aacaaactgc tggcacgttt
     6781 tcaggctgcc aggctcccgg ttcacaatat ttacgccgag tatgtccatt ttgctgacct
     6841 caatgcgccg ttaaacgatg atgagcacgc acaacttgaa cgcctgctga aatatggccc
     6901 ggcactcgcc agccacgccc cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac
     6961 catctctccc tggtcttcga aagcgaccga tattgcccat aactgcgggc tacaacaggt
     7021 aaaccgcctt gagcgcggcg ttgcttacta tatagaagcc ggtacgctga ccaatgaaca
     7081 atggcagcag gttaccgctg aactgcacga ccgcatgatg gaaacggtct tttttgcttt
     7141 agatgatgca gagcagttgt ttgcccacca tcaaccgact ccggttacca gcgttgattt
     7201 gctggggcag ggccgtcagg cgctgatcga cgctaacctg cgtcttggcc tggctctggc
     7261 ggaagatgaa attgactatc tgcaggatgc tttcacaaag cttggtcgta acccgaacga
     7321 catcgagctg tatatgttcg ctcaggcgaa ctctgagcac tgccgccaca aaatttttaa
     7381 tgccgactgg attatcgacg gtgaacagca gccgaaatcg ctgttcaaga tgatcaaaaa
     7441 tactttcgaa accacgccag atcacgttct ctctgcttat aaagataacg ccgccgtaat
     7501 ggaaggttct gaagtgggcc gctactttgc tgaccacgaa acgggccgct acgatttcca
     7561 tcaggaaccg gcgcatattc tgatgaaagt cgaaactcac aaccacccga cggcgatttc
     7621 tccgtggccg ggcgcggcga cgggttccgg cggcgaaatt cgtgatgaag gcgccaccgg
     7681 acgcggtgcg aagccgaagg ctggtctggt tggtttctcc gtttccaacc tgcgaattcc
     7741 tggcttcgaa cagccgtggg aagaagattt cggtaagcct gagcgcattg tcaccgcgct
     7801 ggacatcatg accgaaggcc cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc
     7861 ggcactgaat ggctacttcc gtacttatga agaaaaagtg aacagccaca acggcgaaga
     7921 gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga
     7981 tcacgtacaa aaaggcgaga tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc
     8041 aatgaacatc ggtcttggcg gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc
     8101 cgacctcgac tttgcttccg tacagcgcga caacccggag atggagcgtc gctgccagga
     8161 agtgatcgac cgttgctggc agcttggtga tgccaaccca atcctgttta tccacgacgt
     8221 cggcgctggc ggtctttcta acgcgatgcc ggaactggtg agcgacggcg ggcgcggcgg
     8281 taaatttgaa ctgcgcgata ttctaagcga cgaaccgggc atgagcccgc tggaaatctg
     8341 gtgtaacgaa tcccaggaac gctacgtgct ggcggttgct gccgatcaat taccgctgtt
     8401 tgacgaactg tgtaagcgtg agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga
     8461 actgcatctt tctctgcacg atcgtcattt tgataatcag ccgatcgatc tgccgctgga
     8521 cgtcctgctt ggtaaaacgc cgaagatgac ccgcgatgta caaacgctga aagcgaaggg
     8581 cgatgcgctg gtccgtgaag ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct
     8641 gccgaccgtg gcagagaaaa ccttcctggt gaccatcggc gaccgcagcg taaccggcat
     8701 ggtagcgcgc gatcagatgg tggggccgtg gcaggtgccg gtcgctaact gcgcggtcac
     8761 taccgccagc ctcgacagct actacggtga agcgatggcg attggcgagc gtgcgccggt
     8821 tgcactgctg gatttcgccg cctctgcccg tctggcggtc ggtgaagcgt taaccaatat
     8881 tgccgcaaca cagattggcg atatcaaacg cattaaactt tccgccaact ggatggcggc
     8941 ggcaggccac cctggtgaag atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga
     9001 gctttgtccg gcgctgggcc tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac
     9061 ccgctggcag gaaggtaacg aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc
     9121 tgcatttgcc cgcgtggaag atgtgcgtca caccatcacg ccacagcttt ctaccgaaga
     9181 taacacactg ctgctgattg atttgggcaa aggcaataac gcgctgggcg caacggcgct
     9241 ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat gtacgcgacg tcgcgcaact
     9301 gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag cgtaagctgc tcgcatacca
     9361 cgaccgctct gatggcggcc tgctggtaac gctggcggaa atggcctttg ctggtcattg
     9421 cggcattaac gcggatattg cctctctggg cgacgatcgc ctggcggcgt tgtttaacga
     9481 agaactgggt gcggtgattc aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact
     9541 ggcacagcat gggcttgctg attgtgtcca ttatgtaggg caggcggttt ccggtgaccg
     9601 ttttgtgatt accgccaacg ggcagactgt attcagcgaa agccgcacca cgttgcgtgt
     9661 ctggtgggca gaaactacct ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga
     9721 ccaggagcac caggcgaaat caaacgacgc cgatccgggc ctgaatgtga aactgtcgtt
     9781 cgatatcaac gaagatgtgg cagcaccgta tattgccact ggcgcacgtc cgaaagttgc
     9841 tgtgctgcgt gagcagggcg tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc
     9901 aggctttgat gctatcgacg tgcatatgag tgacctgctg accggacgca cgggcctgga
     9961 agatttccac gccctggtcg cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg
    10021 tgaaggttgg gcgaagtcaa tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt
    10081 cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa
    10141 cctgcgtgaa ctgatcccgg ggagcgagtt gtggccacgt tttgtgcgta acacctccga
    10201 tcgctttgaa gcgcgtttca gcctggttga agtaacccaa agcccgtctc tgctgttgca
    10261 ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat ggtgaagggc gcgtggaagt
    10321 gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg ctggtggcac tgcgctatgt
    10381 cgataacttc ggcaaagtca ctgaaaccta cccggctaac ccgaacggtt caccgaacgg
    10441 tattacggcg gttacgactg aaagtggtcg agtcaccatt atgatgccgc acccggaacg
    10501 tgttttccgt actgtcagca actcctggca tccggaaaac tggggcgagg atggcccatg
    10561 gatgcgcatt ttccgcaatg cgcgtaagca gttggggtaa gtcatcagtt cattggtttt
    10621 gcagcccgga tgcggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    10681 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    10741 aatgattgat tttattgtaa tctaataatt ttatcttaag ttgtctcttt ttagcgacac
    10801 agtggctgat ttacattcaa atagcgaaga agccataaat ccctcaaagc aacgtataat
    10861 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    10921 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    10981 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    11041 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    11101 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    11161 aaacgctggc ccgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    11221 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    11281 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    11341 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgaccct
    11401 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    11461 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    11521 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    11581 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    11641 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    11701 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    11761 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    11821 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    11881 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    11941 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    12001 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    12061 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    12121 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    12181 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    12241 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    12301 gaatccggta acatttgcct tcgcagcagt tcacatggtg cgcgggttta tattgatgtc
    12361 atcaatacag gcacgcccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    12421 cagggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    12481 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    12541 gtttgtttcc gcattgaatt accgtcgtcg aaaaacacga aataatgcta acagcctatc
    12601 ctatcaggct attttatttg tcagttcggc cccgggcagt gctcgaaatc ttcacgtacg
    12661 atatgtacgc tccggtttct ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc
    12721 cactgggata ggctttaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    12781 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagaatcaca
    12841 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    12901 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    12961 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc
    13021 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    13081 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt
    13141 taagtaccga gatcccggca caggttcgcc cactgtatca actctggcgc gacggtcagg
    13201 cgctgcaact gcaactggcg gaagagcgcc aacgttacag taaactccag cagtccagcg
    13261 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    13321 ccacccgcaa gctggaaaac ctgaccgata ttgaacgcca gctctcgacc cgcaaaccga
    13381 ctggaaattt ctcgccggat acgccgcacg agagtgaaaa gcccgcgcca tccacccatg
    13441 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    13501 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    13561 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    13621 ctgcggatgg atgaaatgga cggtatgcag ctatttgctg aaatccagaa agtgcagccg
    13681 ggaatgccag taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    13741 cagcagggcg tttttagttt cctcaccaag cctgtcgata aagatgcgct atatcaggca
    13801 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    13861 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    13921 gacgtcagcg ttttgattaa cggtcagagc ggcaccggga aagagatttt cgcccaggct
    13981 atccacaacg ccagcccgcg caacagcaaa ccatttattg ctattaactg tggcgcattg
    14041 cccgagcaat tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    14101 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gaacgctatt tctcgatgaa
    14161 attggcgata tgcctgcgcc gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    14221 gtgcgcccgc tgggcagtaa ccgcgatatt gatatcaatg tgcggattat ttctgccact
    14281 caccgtgatc tgccaaaagc gatggcgcgc ggggaattcc gcgaagatct ctattaccgc
    14341 ctcaacgttg tcagcctgaa aattccggca ctggcggagc gcacagaaga cattccgcta
    14401 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    14461 tctaccgatg cgatgaaacg cctgatgacc gcgagctggc cgggtaatgt gcgccagttg
    14521 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggtgattag tgatgcgctg
    14581 gtggagcagg cgctggaggg tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat
    14641 cagtttgaac tcaactattt gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac
    14701 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    14761 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    14821 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    14881 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    14941 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    15001 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    15061 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    15121 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    15181 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    15241 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    15301 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    15361 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    15421 ttagcgcgat cggcttcgct cggctgacga tgccaggtgt gcgcggtaaa gcgcggcagt
    15481 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    15541 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    15601 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    15661 ataaagaaat cacctgccgg agcgaccagt ttcacgacat cgccaacatt ggcgtgattg
    15721 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    15781 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    15841 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    15901 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    15961 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    16021 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    16081 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    16141 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    16201 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagggcttca
    16261 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    16321 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    16381 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    16441 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    16501 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    16561 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    16621 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    16681 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    16741 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    16801 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    16861 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    16921 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    16981 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    17041 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    17101 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    17161 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    17221 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    17281 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    17341 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    17401 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    17461 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    17521 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    17581 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga
    17641 tggtagaagt gttccttgag cgcggctaca aagtggtttc cggcggcact gataaccacc
    17701 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    17761 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    17821 tgacctccgg tattcgcgtg ggtactccgg cgattacgcg tcgcggcttc aaagaagcag
    17881 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    17941 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgtttcccg gtttacgcat
    18001 aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaac tttgcaaatt caatatattg caatatccgt gtaggcctga
    18121 taagcgtagc gcatcaggca atcttaggta tgtgatcatc aaggcttcct tcgggaagcc
    18181 tttctacgtt attgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa
    18241 tccccaacac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtt gctttcacgc
    18301 tcggcccttc gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc
    18361 agtgctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt
    18421 tgaggaagtt atacagactc aaaccaatgg cattggcact gcgatcaacc caactggtga
    18481 tccacggttc tccactctac caggcagcga ttaactgcgc tgtagtcagt ttttccggat
    18541 caagctgagt attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac
    18601 ttaacgaggc gacggtttcc aggcagccgt aacgtccaca gtcgcagacg acgccatccg
    18661 gattaacgat ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct
    18721 cgttaatcac gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt
    18781 tctgcgaatt attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca
    18841 tcacccgaat gccgagcttc tcttccagca gatacttcac ctcaaccggc gttgtccacg
    18901 gcgcttgcgg catggtttgc gacacgccgg tcaccggatc aacctgaccg tggattgcca
    18961 gcgccagatt gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga
    19021 tttcggacag cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt
    19081 tcggacttaa acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc
    19141 acagcgtcca gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct
    19201 ggctttcatc gtcgatattc accacccgtt tttcgctttc cagttcctgc aaaatattac
    19261 tgactgccgg aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt
    19321 gccggtacag cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa
    19381 tgcaggctct catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac
    19441 attgccagat tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat
    19501 tgagggcacc atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga
    19561 aaccgggccg caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc
    19621 cggcgcggtt tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc
    19681 ctggcaggcg gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg
    19741 cggtcgggtg catcgcgcgt gggataaagt gaaacagcgc gattttgttt atcacaatga
    19801 agtcattaaa cctgcgctgg tggggctgct ggggccgtgg atttccggtg ggattgagtt
    19861 taactggccg caacatcatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc
    19921 ccatgaagac ggtgcacaga cggtgtgggt aggcgaaacg gagccgatgc atggtttaca
    19981 ggtgatgaca ggtttcaccc tgcgccctgg gcggcgctgg aaatcgccag ccgcgtctat
    20041 aacggcaacg ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggt
    20101 gaagggcatc agagcgtttt cccaccggat ttgactgcgg tgtttgatca cggcaaacgg
    20161 gccgtctccg ctttccccat cgctaccggc acttactaca aagtggacta ctccgctgga
    20221 gtggacattt ctcgctataa aaatgtgccc gttccaacct catatatggc cgaaaaatcg
    20281 cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg gcggtttgct gcacgttgcc
    20341 aaccaccata ttgcgctagg taaaaaacag tggagttggg gacacagtga atttggccag
    20401 gcgtgggata agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt
    20461 tttgccgata accagcccga ttttacctgg cttgatgctt acgaagagaa gcgttttgag
    20521 cagtatttcc tgccttatca ttctctgggc atggtgcaaa atgcctcccg cgatgcggtg
    20581 ataaaactcc agcgtagtga gcaggggatt gagtgggggc tgtatgccat ctctccgttg
    20641 aacggatacc gcctggcgat ccacgaaatc ggcaaatgca acgcgttact tgatgatgcc
    20701 gtggcactga tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgatagg
    20761 ctgaccattg agctttccga tgccgacggc aatattgtac tgagttatca ggaacatcag
    20821 ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac tggcagcaca agacattacc
    20881 agtacagatg aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt
    20941 tcaccgttcg attactacct gcgcggcgtg gcgctggacc cgctggatta tcgctgtaac
    21001 ctggcgctgg cgatgctgga atataaccgc gcagatttcc cgcaagcggt ggcgtatgcc
    21061 agtcaggcgc tgaaacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt
    21121 ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc
    21181 tggcgtgcgg tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg
    21241 gcggcgcgta atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc
    21301 tgcccaacca atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt
    21361 caggacaacg cgcgtttgca gcgcgcgaaa ctgctgcgtg attatccgct gaacgccatt
    21421 ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt
    21481 ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga
    21541 atgcccactc tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt
    21601 tatctacaag ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt
    21661 gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag
    21721 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc
    21781 agctacaaca aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
    21841 gatggctggc gcgggttggc gatccatgcg tggaataagc aacacgatta tgaactggcc
    21901 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
    21961 cgggatttgc tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa
    22021 aataatctgg aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg
    22081 catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg
    22141 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
    22201 tggcagcatc ttgatgccag acagccgcag caggcctgcg aactgcttca tgccgcgctg
    22261 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
    22321 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgac gcgttgttta
    22381 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
    22441 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acaaatcgca
    22501 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
    22561 gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga tttacaacag
    22621 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
    22681 gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
    22741 gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac cctattaact
    22801 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
    22861 aataaaaact ctacaggagg cactatgcct aaaaaaatga gaacaacccg taatctatta
    22921 ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa agaaatgacc
    22981 attggtgcaa tataccttga taccctggga tattacgctg gagtgcgtca gggcgttcag
    23041 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
    23101 atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt
    23161 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
    23221 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
    23281 gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc tgccgccgat
    23341 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
    23401 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc
    23461 gcgcaaattg tcgctaatca ggaagggact gttttaaata aagcgatttc cgttggtgaa
    23521 aaactgatta tctccacgcc ggatctcaac gccattatgg gggagtcggg cggtgcgaca
    23581 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
    23641 tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta
    23701 gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt gaaggttatc
    23761 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
    23821 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
    23881 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
    23941 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
    24001 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
    24061 cggcagcgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
    24121 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggaatt
    24181 aagtctggtg gaagggctga cggtggcaga aaacctctgc cttggtcaat ggcctcgccg
    24241 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
    24301 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
    24361 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacccac
    24421 cagctcgctt gccagtgcag aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc
    24481 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaatccgcc gcattgcctc
    24541 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
    24601 cacgcatcat attgtgtcgc taatgctagg gcgtgatcac gttgatattg cgccggttgc
    24661 ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc ataagcccaa
    24721 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
    24781 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg
    24841 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
    24901 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
    24961 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag tgctgcaatg
    25021 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgctag
    25081 tagcgaaacg cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg
    25141 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
    25201 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
    25261 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt
    25321 acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
    25381 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
    25441 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
    25501 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
    25561 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
    25621 tgccgcctgg gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc
    25681 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
    25741 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
    25801 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
    25861 cggaatgggg ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct
    25921 ggactggctg ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt
    25981 gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt
    26041 tggcggtaat gccacggcgg cgcagttgtg cggtattaac gttcgtcggg tgcgcattct
    26101 tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgctgg cggcacgcct
    26161 cggttccggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt
    26221 ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt
    26281 gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca
    26341 gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca
    26401 gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg
    26461 aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca
    26521 ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta
    26581 ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa
    26641 cactgtgcca cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat
    26701 gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac
    26761 cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt
    26821 cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat
    26881 gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac
    26941 gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc
    27001 gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg
    27061 atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa
    27121 cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc aaccaccgaa
    27181 ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc gctcgattgt
    27241 tctggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg gggacgggtg
    27301 gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat
    27361 caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccac
    27421 gatttgactg actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa
    27481 caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac
    27541 ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc
    27601 tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct
    27661 tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat
    27721 ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg gcgtgagtat caactgcaac
    27781 cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat
    27841 gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc
    27901 actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg acgctctctg
    27961 tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt
    28021 tgtcaccaca aacgcggatt caggcgcagg cgagcggtta ctggctggaa cgggagcagt
    28081 ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc
    28141 cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg
    28201 agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt
    28261 tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg
    28321 aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc
    28381 tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg ctgtaattta
    28441 ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag
    28501 aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct
    28561 tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac
    28621 caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa
    28681 agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca
    28741 gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca
    28801 ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca
    28861 aaacggggcg ggcagctcca aaatcaaaat aacg
//
LOCUS       DAZOZY010000032        28264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZOZY010000032 DAZOZY010000000
VERSION     DAZOZY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.032"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHURQ_001221"
     CDS             complement(<1..215)
                     /locus_tag="ACHURQ_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0823051.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHURQ_001222"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHURQ_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR0823052.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHURQ_001223"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHURQ_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR0823053.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHURQ_001224"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHURQ_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR0823054.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHURQ_001225"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHURQ_001225"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR0823055.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHURQ_001226"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHURQ_001226"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR0823056.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHURQ_001227"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHURQ_001227"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR0823057.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHURQ_001228"
     CDS             complement(4604..5326)
                     /locus_tag="ACHURQ_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0823058.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHURQ_001229"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHURQ_001229"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0823059.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHURQ_001230"
     CDS             6526..6744
                     /locus_tag="ACHURQ_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR0823060.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHURQ_001231"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHURQ_001231"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0823061.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHURQ_001232"
     CDS             complement(7478..7678)
                     /locus_tag="ACHURQ_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR0823062.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHURQ_001233"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHURQ_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR0823063.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHURQ_001234"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHURQ_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR0823064.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHURQ_001235"
     CDS             10175..12088
                     /locus_tag="ACHURQ_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0823065.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHURQ_001236"
     CDS             12088..13110
                     /locus_tag="ACHURQ_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0823066.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHURQ_001237"
     CDS             13104..13322
                     /locus_tag="ACHURQ_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR0823067.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHURQ_001238"
     CDS             13376..14245
                     /locus_tag="ACHURQ_001238"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR0823068.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHURQ_001239"
     CDS             complement(14300..14704)
                     /locus_tag="ACHURQ_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR0823069.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHURQ_001240"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHURQ_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR0823070.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHURQ_001241"
     CDS             15689..17779
                     /locus_tag="ACHURQ_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR0823071.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHURQ_001242"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHURQ_001242"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR0823072.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHURQ_001243"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHURQ_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR0823073.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHURQ_001244"
     CDS             complement(19747..20349)
                     /locus_tag="ACHURQ_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR0823074.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHURQ_001245"
     CDS             complement(20339..20506)
                     /locus_tag="ACHURQ_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR0823075.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHURQ_001246"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHURQ_001246"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR0823076.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHURQ_001247"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHURQ_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR0823077.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHURQ_001248"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHURQ_001248"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR0823078.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHURQ_001249"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHURQ_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR0823079.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHURQ_001250"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHURQ_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR0823080.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHURQ_001251"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHURQ_001251"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR0823081.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6838 a         7592 c         7131 g         6703 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagta gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tctt
//
LOCUS       DAZOZY010000033        28044 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZOZY010000033 DAZOZY010000000
VERSION     DAZOZY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.033"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHURQ_001252"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHURQ_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR0823082.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHURQ_001253"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHURQ_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHURQ_001254"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHURQ_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHURQ_001255"
     CDS             complement(1702..3318)
                     /locus_tag="ACHURQ_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR0823083.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHURQ_001256"
     CDS             3614..4330
                     /locus_tag="ACHURQ_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0823084.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHURQ_001257"
     CDS             complement(4327..5988)
                     /locus_tag="ACHURQ_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR0823085.1"
                     /translation="MNDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHURQ_001258"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHURQ_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR0823086.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHURQ_001259"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHURQ_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR0823087.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHURQ_001260"
     CDS             7443..7775
                     /locus_tag="ACHURQ_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0823088.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHURQ_001261"
     CDS             complement(7777..8991)
                     /locus_tag="ACHURQ_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR0823089.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHURQ_001262"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHURQ_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR0823090.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHURQ_001263"
     CDS             complement(10153..11490)
                     /locus_tag="ACHURQ_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0823091.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHURQ_001264"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHURQ_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHURQ_001265"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHURQ_001265"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR0823092.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHURQ_001266"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHURQ_001266"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR0823093.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHURQ_001267"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHURQ_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR0823094.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHURQ_001268"
     CDS             15153..15809
                     /locus_tag="ACHURQ_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823095.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHURQ_001269"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHURQ_001269"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR0823096.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHURQ_001270"
     CDS             complement(16782..17180)
                     /locus_tag="ACHURQ_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR0823097.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHURQ_001271"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHURQ_001271"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR0823098.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHURQ_001272"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHURQ_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR0823099.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHURQ_001273"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHURQ_001273"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR0823100.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHURQ_001274"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHURQ_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR0823101.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHURQ_001275"
     CDS             23741..23821
                     /locus_tag="ACHURQ_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823102.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHURQ_001276"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHURQ_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR0823103.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHURQ_001277"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHURQ_001277"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR0823104.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHURQ_001278"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHURQ_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR0823105.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHURQ_001279"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHURQ_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR0823106.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHURQ_001280"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHURQ_001280"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR0823107.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6760 a         7565 c         7158 g         6561 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcattcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca actgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa tatt
//
LOCUS       DAZOZY010000034        27793 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZOZY010000034 DAZOZY010000000
VERSION     DAZOZY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27793
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.034"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            72..734
                     /gene="acrS"
                     /locus_tag="ACHURQ_001281"
     CDS             72..734
                     /gene="acrS"
                     /locus_tag="ACHURQ_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR0823108.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(737..916)
                     /locus_tag="ACHURQ_001282"
     CDS             complement(737..916)
                     /locus_tag="ACHURQ_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR0823109.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(1000..1884)
                     /gene="yhdJ"
                     /locus_tag="ACHURQ_001283"
     CDS             complement(1000..1884)
                     /gene="yhdJ"
                     /locus_tag="ACHURQ_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR0823110.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1970..2266)
                     /gene="fis"
                     /locus_tag="ACHURQ_001284"
     CDS             complement(1970..2266)
                     /gene="fis"
                     /locus_tag="ACHURQ_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR0823111.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2292..3257)
                     /gene="dusB"
                     /locus_tag="ACHURQ_001285"
     CDS             complement(2292..3257)
                     /gene="dusB"
                     /locus_tag="ACHURQ_001285"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR0823112.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3586..4467)
                     /gene="prmA"
                     /locus_tag="ACHURQ_001286"
     CDS             complement(3586..4467)
                     /gene="prmA"
                     /locus_tag="ACHURQ_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR0823113.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4479..5930)
                     /gene="panF"
                     /locus_tag="ACHURQ_001287"
     CDS             complement(4479..5930)
                     /gene="panF"
                     /locus_tag="ACHURQ_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR0823114.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5920..6162)
                     /locus_tag="ACHURQ_001288"
     CDS             complement(5920..6162)
                     /locus_tag="ACHURQ_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR0823115.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6271..7620)
                     /gene="accC"
                     /locus_tag="ACHURQ_001289"
     CDS             complement(6271..7620)
                     /gene="accC"
                     /locus_tag="ACHURQ_001289"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR0823116.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7631..8101)
                     /gene="accB"
                     /locus_tag="ACHURQ_001290"
     CDS             complement(7631..8101)
                     /gene="accB"
                     /locus_tag="ACHURQ_001290"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR0823117.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(9079..10053)
                     /locus_tag="ACHURQ_001291"
     CDS             complement(9079..10053)
                     /locus_tag="ACHURQ_001291"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR0823118.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10205..12145
                     /gene="csrD"
                     /locus_tag="ACHURQ_001292"
     CDS             10205..12145
                     /gene="csrD"
                     /locus_tag="ACHURQ_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR0823119.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12450..13493
                     /gene="mreB"
                     /locus_tag="ACHURQ_001293"
     CDS             12450..13493
                     /gene="mreB"
                     /locus_tag="ACHURQ_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR0823120.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13559..14662
                     /gene="mreC"
                     /locus_tag="ACHURQ_001294"
     CDS             13559..14662
                     /gene="mreC"
                     /locus_tag="ACHURQ_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR0823121.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14662..15150
                     /gene="mreD"
                     /locus_tag="ACHURQ_001295"
     CDS             14662..15150
                     /gene="mreD"
                     /locus_tag="ACHURQ_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR0823122.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15159..15752
                     /gene="yhdE"
                     /locus_tag="ACHURQ_001296"
     CDS             15159..15752
                     /gene="yhdE"
                     /locus_tag="ACHURQ_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR0823123.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15742..17211
                     /gene="rng"
                     /locus_tag="ACHURQ_001297"
     CDS             15742..17211
                     /gene="rng"
                     /locus_tag="ACHURQ_001297"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR0823124.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17279..21079
                     /gene="yhdP"
                     /locus_tag="ACHURQ_001298"
     CDS             17279..21079
                     /gene="yhdP"
                     /locus_tag="ACHURQ_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR0823125.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21235..22680
                     /gene="tldD"
                     /locus_tag="ACHURQ_001299"
     CDS             21235..22680
                     /gene="tldD"
                     /locus_tag="ACHURQ_001299"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR0823126.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22808..23737)
                     /gene="aaeR"
                     /locus_tag="ACHURQ_001300"
     CDS             complement(22808..23737)
                     /gene="aaeR"
                     /locus_tag="ACHURQ_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR0823127.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23920..24123
                     /gene="aaeX"
                     /locus_tag="ACHURQ_001301"
     CDS             23920..24123
                     /gene="aaeX"
                     /locus_tag="ACHURQ_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR0823128.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24131..25063
                     /gene="aaeA"
                     /locus_tag="ACHURQ_001302"
     CDS             24131..25063
                     /gene="aaeA"
                     /locus_tag="ACHURQ_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR0823129.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25069..27036
                     /gene="aaeB"
                     /locus_tag="ACHURQ_001303"
     CDS             25069..27036
                     /gene="aaeB"
                     /locus_tag="ACHURQ_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR0823130.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27128..27400
                     /locus_tag="ACHURQ_001304"
     CDS             27128..27400
                     /locus_tag="ACHURQ_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR0823131.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27456..27719)
                     /gene="yhcN"
                     /locus_tag="ACHURQ_001305"
     CDS             complement(27456..27719)
                     /gene="yhcN"
                     /locus_tag="ACHURQ_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR0823132.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6671 a         6963 c         7384 g         6775 t
ORIGIN      
        1 ttagatgata gattaacctt cgctattttc tcaccctgtg tcgaatatat ttatttcctg
       61 aataattaat catggcaaaa agaaccaaag ccgaagctct gaagacccgg caagaactga
      121 ttgaaactgc catcgcccag tttgcgcaac atggcgtaag caagacgacg ttcaacgaca
      181 ttgccgatgc cgctaacgtt acgcgtggcg ctatctactg gcacttcgag aacaagaccc
      241 aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc caggaacact
      301 tgacggctgg attagagcat gacccgtttc aacaattgcg tgaaaaattg attgtcggct
      361 tgcaatatat tgccaaaatt ccccgccagc aggcgttgct gaaaatctta tatcacaaat
      421 gtgaatttaa tgatgggatg ctggccgagg gagtgatacg cgaaaagatg ggctttaatc
      481 cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta gcaaataacc
      541 tcgatttaga tgttgtgatg attattattg atggtgcctt cagcggaatt gttcaaaact
      601 ggttaatgaa tatggcgggt tatgatcttt ataaacaagc acccgcgctg gtcgataacg
      661 tattaagaat gttcatgcca gatgaaaaca taacgaaatt aattcatcaa acgaatgaat
      721 taagtgtcat gtaaattcac attctgtcct gaaaattcag ttcgtcacag ttaaccagtt
      781 ttagtggata aactgagttc atattacggc atttgtctgt ttctgttgtc agcagttcaa
      841 tccactgcat cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg acgagccacc
      901 aatacttgcg aatcattacc tgagcctgtg catgtttaac caagtctggc aggaaatata
      961 cacgaaatga ataatctgaa aaccaggctt acacttcctt tactttgcaa tgagatcggg
     1021 gtcaacttcg cttaaccggc ttcgttttga caggttgccg gtctttcttt ttttcacttt
     1081 cgccagttct tccgcggagt aatgcgacgc gacatccagc cgtcgaagcc ccattttgat
     1141 gtactcgctg ttgatctcaa taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc
     1201 ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg cctgggttgg aagaggcgag
     1261 aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg tggttctcat attcatccat
     1321 caaataacgc acgcgcggaa aatcccagac gttacccggt actttttgat gattgtatgg
     1381 ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt tggcttctac
     1441 cagaatagca tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg
     1501 ttcgtacatg gagccgtagt gttttttcgc ctgtactcca gaactgtcat atgaccagac
     1561 gatgcgactt ttgatggtaa aaagcttgcg gcactggaga tcgataaagg gcatgttttc
     1621 cgtactgttc ataatgtaca tgctgccctg ctttttcaga acgcggtggc actctacaat
     1681 cacttcaaac agccagtcga taaacagatc ttctttccag gcttcgatca gaccatcaaa
     1741 atttttaccg atgttatacg gtgggtcggc aaagatcaga tcgacgcttt cagtaggtag
     1801 ctttttaagt tcggcaaggg catcaccgtg aataatggtc ttagcttcat taccaaaccg
     1861 ggtcggttca catcctgttc tcatggtcac tccctttgtg acacctataa aaaaggcgct
     1921 tccccatgcc gagtagcgcc tttttaatca agcatttagc taacctgaat tagttcatgc
     1981 cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc agcgcagcac
     2041 gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt tctacttcag
     2101 ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca aaatagttct
     2161 tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc tgagagttaa
     2221 cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt ctgtcagctc
     2281 tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag ctgttcgctg
     2341 gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg ttcctggaga
     2401 taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata aaagtcatgc
     2461 agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag cgggggcagc
     2521 aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct tccctgagct
     2581 gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct ggctttaagc
     2641 gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt aactgcccga
     2701 atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc atgaatggtc
     2761 agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt acggtgttcc
     2821 ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt gacgacctcg
     2881 gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc gagcttgcga
     2941 ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc gctttccacg
     3001 ttaatacgtg ctgcatctgc catttctttc ggatcgctac cagcaatttg cacggtgcga
     3061 ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc ccaaacctgt
     3121 gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta gcacaacgtc
     3181 cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt tctgagctga
     3241 tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg cggcgtatat
     3301 tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga gatggatcaa
     3361 agaattgcat ttaaaatgag cgtggtgaga taattactca taaaaatcat catattagaa
     3421 aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt ttctttatga
     3481 atgaaaattt tggcacgcaa atttgcgtaa ataaccggca aatttacgtg ccttttgtga
     3541 gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc gaaggttaat tcttacgacc
     3601 ggtaatgcgg caccactctt ctttttccac gaccgggtcc agtgcgaagc tgtcgacata
     3661 agcttcacaa acgctctctg cctggcttgc cagaataccg gaaaggccca gcaaaccgcc
     3721 tgaaaccggc aggacgctga ttaacggtgc cagttcacgt aatgggcctg caaggatgtt
     3781 agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt agagttccag
     3841 acggtcagaa acgccattac gttcggcgtt gtcgcggcta gcctgaatcg cctgcggatc
     3901 gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatag ccagaatgcc
     3961 ggaaccacag ccaaagtcga tgactatttt accggttaaa tcgaggctgt cgagccactg
     4021 caggcacaga gaggtagttg gatgggtacc cgtaccaaac gccagccctg gatctaacat
     4081 cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc acagtcgttc
     4141 accaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt tatcttccag
     4201 ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca gaatcgccac
     4261 cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc acatcggtgt cgccccacag
     4321 gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat
     4381 agaaacggca cccgcttcca tcagcgcatc gctaagatct tcagcgttcg cgccggtggt
     4441 gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt agtcaaaacg
     4501 gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag caaacttagt
     4561 agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt cgcgagtacg
     4621 gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc
     4681 cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc gaaggccagc
     4741 aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag caacgcgccg
     4801 agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg catttgatcc
     4861 ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgtag caattgggcg
     4921 ttaattgtcg acatgatcgc agccataggt gcagccagaa agatcccggc agcaaacggt
     4981 ggcagcactt ttaccattaa cgtcgggatc accagatccg gtacggtgag atcggggatc
     5041 actgcacgac ctaacgctcc ggctaggtgc ataccgaaca tcaggattgc gactacaatc
     5101 gtaccgatga tgatcccccg gtgtaccgct ttgctgtctt tataagagat acagcgcacc
     5161 gcagtatgcg gcaggccaat cacgccaaag cacaccagta cccagaacga cgtcataaag
     5221 gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc gatggtttgc
     5281 aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat aagcagcaca
     5341 acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag
     5401 ccgccaaagg cggtatacaa cgcaatgctg ataccgaaaa tcagcagccc ggtttcataa
     5461 ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac ggtcattgcc
     5521 ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg actctggtag
     5581 cgggcaaaca gcatgtcgtt cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac
     5641 ttcttgccga gaataccgag tgaaagccag actgcaggaa gctgaatcat cgccagcaat
     5701 acccagccca gcccgtattt ataagcagct cctggcccgc cgataaacga actggcactg
     5761 atataggtcg cggtgagcgt catcgccagc acaataccgc ccatagagcg gctgccgagg
     5821 aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac cgagataccg
     5881 aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat cgtcatcctc
     5941 cagtgggata tcgcgataga taaatttcac catcgcccag cacagtccaa taaacagtag
     6001 cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac
     6061 gccaggtaag taagcggcta ctaaccaaac tgccagatac aaaagggtca gccccagcgc
     6121 ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc cctgatgggt
     6181 gaagaaagcg aggattgtac cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg
     6241 aaaatccggc cttttgacgc ttttgcagtc ttatttttcc tgaagaccga gttttttctc
     6301 cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga tgcggatctg
     6361 cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg
     6421 ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca ttgagtcata
     6481 gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgtacgc caaaaccgcc
     6541 aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc
     6601 gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct tgatcgacag
     6661 cggttgaccg gcagcgatac ggagctgttc tttgatcagg tcaacgccag tgatcatttc
     6721 ggtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga actcgccgtt
     6781 ttcgaacaga aactcgaaag tacctgcacc gcgatagccg atatcaacac acgctttagc
     6841 gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg gcgcttcttc
     6901 gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat agatagcgtt
     6961 gccctgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca ggtatttctc
     7021 catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca tggagatgga
     7081 ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc cgccgccgga
     7141 ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt tatccatatc
     7201 gtcgcccaac gggccgtcag aacccggtac gcaagggacg cccgcttttt tcatcgccgc
     7261 gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc cgatgaagat
     7321 aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga agccgtaacc
     7381 cggatggatt gctactgcgc cggtgatttc agcggcgctg atgattgccg ggatgttcag
     7441 ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca gtaatacgtg
     7501 ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca gttctttaca
     7561 ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt tatccagcat
     7621 gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt
     7681 cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc atcattttca
     7741 tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact tcgatgaacg
     7801 cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggaa cgtacgatgt
     7861 gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc ggagcggctg
     7921 cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc atcacaggga
     7981 aacttgcggc aggagctgca cggctaatgc gtactgactc ttcgccttca gaaatttcca
     8041 gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta cgaatatcca
     8101 tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt
     8161 aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc gggacgccgg
     8221 tctattttgc cccagagtat caatgttaga cttgacggac attgtgcagc gatctgctgc
     8281 tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt ttccgcagcg
     8341 caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag caattggcag
     8401 ctaaatactg gtctcatcag ggaagataat caacagctaa catgtaaata accttcaaca
     8461 ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc gacggaactt
     8521 cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg gctgcaatga
     8581 gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa gatagacgac
     8641 attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga tagatgtgaa
     8701 tgctaaagca cgcaaaatca accgactgac aattctcagc attaaatagg ttcgagtggt
     8761 taatttgtag tcaaataaaa tcagattatt caccccatgg taccagaaat cagtaacagg
     8821 ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg aggcgtatca
     8881 ataatggctg tttagaggag tatcaatgcg catcaatttg tggtcagtcg aaatcatgac
     8941 tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga aagctgtccg
     9001 gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt ccctctctta
     9061 ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga tctggttatt
     9121 aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg ccgcctgggt
     9181 atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg
     9241 tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa atggcatgac
     9301 cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc cgccgtaatt
     9361 catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg ccccagccca
     9421 gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa tacggctggc
     9481 acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa cgacctgata
     9541 acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg cacccgtcac
     9601 gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca cacacagcat
     9661 ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc cttgcggcat
     9721 ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc agtggttttc
     9781 accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct
     9841 ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac ggatgatttt
     9901 tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt gaacatcgac
     9961 cgtgacatcg ccctccggca ggcgactttc gtccagagtc tgtactgatg cgagagtttt
    10021 gccatcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc atgatggatt
    10081 ggaaaataat ttctgaagat tatactcgct aatggaaaag caaagccgat gaagcgcaat
    10141 aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg gagttaacac
    10201 aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg ggttaacaat
    10261 ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt ataagtttag
    10321 tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga atgacttcag
    10381 cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc gtgtagacct
    10441 gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc gtccggttgg
    10501 ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg ggatgtcgct
    10561 gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga ctaccgcgcc
    10621 gctcacgggg gcgattggct ttatcattgt tatgctcttc ctggcggtac gctggttaca
    10681 acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct taaacggtga
    10741 gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca gcagtgcgct
    10801 agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct
    10861 gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac tctttttcga
    10921 taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg ggatcgtgat
    10981 gatgattcgt ctgccggatt tcaatatgtt gagcgatact tgggggcaca gccaggttga
    11041 agaacagttc ttcactctga ctaatctgct gtcgacattt atgatgcgct accctggcgc
    11101 actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa
    11161 agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc cgaacaataa
    11221 aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta gtggtcagga
    11281 taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat tgcagggcgg
    11341 caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg
    11401 gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc
    11461 ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa
    11521 tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct tatcggaaga
    11581 gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc cagaggaaaa
    11641 tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc gttggctgcg
    11701 cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg aacttgcaga
    11761 ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag tgaatgcttt
    11821 aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca gttggatcaa
    11881 agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca ttgagaagcg
    11941 aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga ccagcaccca
    12001 cgtttacgcc accggcgtgc gttcgcgaag cgagtggcag accctgattc agcgcggtgt
    12061 tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa
    12121 atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt agaataacgc
    12181 gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc acatatgcag
    12241 atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat taagcctttc
    12301 tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc accgtagcag
    12361 atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc
    12421 cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc atgttttcca
    12481 atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa ggacaaggca
    12541 tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa
    12601 gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg ggcaatattg
    12661 ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc
    12721 tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc ccgcgcgttc
    12781 tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc
    12841 agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc gcaattggtg
    12901 ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt ggtggtacca
    12961 ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg cgcattggtg
    13021 gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt tctctgatcg
    13081 gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg ggcgatgaag
    13141 tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc ggttttaccc
    13201 tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg
    13261 taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc gagcgcggca
    13321 tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta atggaagaaa
    13381 ccggcattcc agtagttgtt gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca
    13441 aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag taatcggatg
    13501 caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg cataacttat
    13561 gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg cggtgctggt
    13621 ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa tccgtactta
    13681 tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg aattgctgga
    13741 tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg
    13801 tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca aacaggagaa
    13861 cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga aaatggtgac
    13921 tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg ataaaggtag
    13981 cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg ttggtcaggt
    14041 ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct
    14101 gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg gttgtacgga
    14161 tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg atgtgctggt
    14221 gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg tctcttccgt
    14281 aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg caggactgca
    14341 acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta acccgatgac
    14401 gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc cgcaggtatt
    14461 gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc
    14521 gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgcgc ccacacagcc
    14581 tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc aaccgcctgc
    14641 gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgttg ggtaatctgg
    14701 ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa cctgattgtt
    14761 ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt gcctcatcgc
    14821 gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag cggctcgacg
    14881 cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct gaaataccag
    14941 cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct ttcgctggtg
    15001 gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt cagaccggaa
    15061 gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt gctgatgcgt
    15121 aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg tatttagctt
    15181 ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg tgtgaccttt gaacgtattg
    15241 ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc
    15301 tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc ccggtgctgg
    15361 gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc gacgcagagc
    15421 atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg acggcagtgg
    15481 cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg actttcagaa
    15541 cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta gataaagcag
    15601 gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat ggcagctatc
    15661 acgccgtagt cggcttacct ctggttgaaa cgtatgaatt attaagtaat tttaacgcac
    15721 tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac gccttcggaa
    15781 acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg
    15841 cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg
    15901 caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc atccgatatc
    15961 atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc
    16021 tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga tccgcttggc
    16081 actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct ggtctttatg
    16141 ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga acgtgaacgc
    16201 ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat ccgtaccgca
    16261 gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa acgcgtctgg
    16321 accaaagtta tggagcgtaa aaagcgcccg cagacccgtt atcagctgta cggcgaactg
    16381 gcgctggctc agcgtgttct gcgtgatttc gccgacgccg aactggaccg cattcgcgtt
    16441 gactcacgcc tgacttacga agcattactt gagttcacct cggagtacat tcccgagatg
    16501 accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt tgatgtcgaa
    16561 aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg ttatctcatt
    16621 atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat
    16681 cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc tatcgctcgc
    16741 cagttacggt tgcgtaatct gggcgggatt atcattattg atttcatcga tatgaataat
    16801 gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa agaccgggtg
    16861 aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg taaacgcacc
    16921 cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg
    16981 gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg tgtccaccat
    17041 gcttacgact ccgaccgttt cctggtctat gcttctccgg cagtagccga agccttgaaa
    17101 ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta
    17161 caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta aacagatgct
    17221 gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg agtgacgggt
    17281 gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga tcgctgccct
    17341 gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc cggaaatcct
    17401 caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct cagccagctg
    17461 gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa aagatggcgg
    17521 cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc tgttacatat
    17581 gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca acactcctat
    17641 caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc tgtttcttcg
    17701 tcaatttgac catttcgatc tccgcgacag tgaagtcagt ttcctgacgc catccggtca
    17761 gcgtgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc gacaccgtgc
    17821 ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc aggtgcgcat
    17881 ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac aggcggatga
    17941 catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc tggaaacggc
    18001 acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt
    18061 ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata cgctgtcggt
    18121 ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct ctattcccga
    18181 tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc tggcctggat
    18241 accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc
    18301 cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga aactttcacc
    18361 tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca ctctggcgct
    18421 ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga gcgatctggc
    18481 ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc tttccggcag
    18541 cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt
    18601 attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc tgaacaatga
    18661 caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg
    18721 cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta ttctggctgg
    18781 catcagtacc gatgatggtt cacaagcctg gcactatttc ccggaaaact tgatgggtaa
    18841 agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata acgcgacgct
    18901 ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc agtttgaagt
    18961 gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg cattaactaa
    19021 ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg atggcgttaa
    19081 tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact cgaaagaaaa
    19141 actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt actttgatga
    19201 gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt
    19261 gaatgctcgc ttacatcttg atatcccgct gaacggcgag ctggtaaccg cgaaaggtga
    19321 agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc tgaaaaattt
    19381 gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga cagcaagctg
    19441 gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag cctaccaggt
    19501 tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt
    19561 gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta
    19621 tcatgctggc gcgacctata acgtagagct gaacggcgat ctgaataatg tgagtagtca
    19681 cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg ttaaggttga
    19741 tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc atttcaatag
    19801 ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg cagacagtaa
    19861 aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc cgatgaatgg
    19921 tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag
    19981 tttcccacaa tacataacgt tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa
    20041 taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg cgcaagggcg
    20101 tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga atatcaaata
    20161 tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt
    20221 cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac gatgcacaga
    20281 gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca tttctggcga
    20341 tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta ctgccgatgg
    20401 tgaatgggtt aataatccgg ggaatgaacg tacctcgctg aaaggaaaac tgcgcgggca
    20461 gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc agtcgtcatt
    20521 taatgtggat tacgatttac actggcgcaa agcaccctgg cagccagatg aagcgacgtt
    20581 gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca ataccggaca
    20641 tgccgggcaa ttgctgcgct tattgagcgt agatgccctg atgcgtaagc tgcgttttga
    20701 tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca ccgcgtggat
    20761 taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg cggatatcgc
    20821 catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg ttgtcgcacc
    20881 agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc
    20941 ggcagttttt gccgctagta aagtgctggg gccgctgtgg agcaaagtct ccattttgcg
    21001 ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg
    21061 taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc atatgataat
    21121 cgttgccaaa ggccaacgag ccagaacata accgtaggcc ggatagggcg ttcatgccgc
    21181 atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa aacgatgagt
    21241 ctgaacctgg taagtgaaca attgctagcg gcgaacagcc tgaaacatca ggacttgttc
    21301 gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg
    21361 agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc ttacaacatc
    21421 gatcagggcg ttggcgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac
    21481 caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat
    21541 agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt gtatacctcg
    21601 gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat
    21661 aaggttgctc gcgaagcgga caagcgcgta caggaagtga ctgccagcct cagtggtgtc
    21721 tatgaattaa ttctggttgc ggccaccgac ggcacgctag cggcggatgt ccgtccgctg
    21781 gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg cggtgccagt
    21841 ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg cgaagtccgt
    21901 gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc tgccgttgct
    21961 gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat
    22021 gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc agtatttagt
    22081 gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg
    22141 gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca gtacaacgtg
    22201 ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc gcgtttgatg
    22261 gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc catgccgcgt
    22321 atgactaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat tgaatccgtt
    22381 gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatatcac ctccggcaaa
    22441 ttcgttttct ccacttcaga agcatatctg attgaaaacg gtaaagtaac gaagccggtg
    22501 aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc agcagatttc gatggttggc
    22561 aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca aagtttgccg
    22621 gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg tactgcgtaa
    22681 taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg gtcccctcgc
    22741 tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg taacagtaca
    22801 atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa
    22861 caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc ttatcttttt
    22921 cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc aggatctcca
    22981 gctcaccgcg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg atcccggcac
    23041 ccgccgtcag ccagcgcacc agcgtcatcg gatcattagt cacaaatctt ccttgtggga
    23101 tcaggcgagt cgagatccct tccggtgcga tcagttcaaa ttcattgtcg ggccgcacgc
    23161 tgtattcaag ccatgaatga ctactcaaat cggcgggttt ttccggtatg ccgtattgtg
    23221 tgagatagct tttcgcggcg cacaccacca tcggcatcgc gcccagacgg cgggaaaaca
    23281 ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt
    23341 cgggggctgg aattccggta accagattga cgctcaaacc tgggtattct ttcagcattt
    23401 ttgctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca atgcgcagcg
    23461 tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc acttcatgaa
    23521 gcatccgacg gcagccctgg tagtaaattc tgccggcttc ggtcaggcca atgctgcgtg
    23581 tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc gtctgactga
    23641 tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca aattcaacta
    23701 ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac ttaaaagtga
    23761 tttagatcac ataatataga taacagcata acagttacgc taatatatta aatatcaatc
    23821 tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac agatgctgaa
    23881 aataataacg cctgctctct ctttacaacc aaggtcaaca tgagtctgtt tcccgttatc
    23941 gtggtgtttg gactgtcctt cccaccgata ttttttgaat tgcttttatc actggcgatt
    24001 ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt ctggcatccg
    24061 gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg actgttcgtt
    24121 tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt
    24181 agtcattcta gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg
    24241 gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact
    24301 cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac
    24361 catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcttatta
    24421 tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca
    24481 ggcgatgtct cgcgaagaga tcgaccaggc caacaacgta ctacaaacgg ttctgcatca
    24541 gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat
    24601 ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac
    24661 tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat
    24721 ggaagaaact aagctggaag gggggcgtcc ggggtatcgt gcagagatca cgccgcttgg
    24781 cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gcaggggtca ccaacgccag
    24841 cagcacgcgt gacgacaaag ggatggcgac tatagactct aatcttgaat gggtgcgtct
    24901 tgcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc
    24961 gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc
    25021 gttcttccgt aaaatggccc atcgcctgcg tgagtttggt taatcacgat gggtattttc
    25081 tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg
    25141 ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact gacagcggcg
    25201 attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc
    25261 tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg actggtgatc
    25321 atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat ctgggccggt
    25381 ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg ggggctggcc
    25441 ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct tacgccgcag
    25501 tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat tatggcggat
    25561 ttgctctttt ctccgcgatc gatcaaacaa gaagtagatc gagagctgga aagtttgctg
    25621 gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga agttgtcgat
    25681 aaagcctggg gcgacctggt gcgacgcacc acggcactac aaggcatgcg cagcaacctg
    25741 aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta
    25801 tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac gcgcccggaa
    25861 ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc
    25921 cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ccggggaacg ggaaacgcct
    25981 gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt
    26041 atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga accggaagta
    26101 aaagtagagt cagccgaacg tcatcatgca atggttaact tctggcgaac cacactttcc
    26161 tgcattctgg gcacgctttt ctggctgtgg acgggctgga cttccggcag tggtgcaatg
    26221 gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg catggtggcg
    26281 atcgacttta tctatgggac gctggccgcg ctgccgttag ggctgctcta ctttttggtg
    26341 attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc agtgctggga
    26401 ttcttcctcg gtatagaagt acagaaacgg cgactgggct cgatgggggc actggccagc
    26461 accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca gtttctcgac
    26521 agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg
    26581 gataaatcgc gcgacaggac tggacgtgta ctgcttaatc agtttgtttt tgccgctgtt
    26641 tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc actttatcag
    26701 cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg cctggcgctg
    26761 acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa cgaggattta
    26821 tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat
    26881 gataaacgtc gtcggtactt tggccagttg ctggaagaac tggaaatcta ccaggaaaag
    26941 ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct ggcggggatg
    27001 ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc caaaagcgtc
    27061 ggttttttca tggctatact tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg
    27121 gcaacagatg aatatttata cctttgattt tgatgagatt gagagtcagg aggattttta
    27181 tcgtgacttt agccaaacct ttggtctggc taaagataag gtacgcgatc tcgactcact
    27241 atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat ttgttcatct
    27301 gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg aggcagagga
    27361 agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa agcccccgaa
    27421 ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc agccgtagcg
    27481 tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc tttctcttcc
    27541 gcttttttgt tcagcatttc acgcatatcc attggcgaag acgccacacc acttacggat
    27601 acggtcccga ttgcttcacg attttgtgct tgtgcagtat caatggagtc ggcagcgaat
    27661 gcaccgaaag agagaacaga aagtacgctt aatgcagcta cagtggtttt gattttcatg
    27721 atttttacct cgacataatc ttttagctgg gcctttgttt cgtgacccat atcacaaaat
    27781 caagcataca cta
//
LOCUS       DAZOZY010000035        27108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZOZY010000035 DAZOZY010000000
VERSION     DAZOZY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.035"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..924
                     /locus_tag="ACHURQ_001306"
     CDS             <1..924
                     /locus_tag="ACHURQ_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0823133.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHURQ_001307"
     CDS             complement(967..1827)
                     /gene="rsmI"
                     /locus_tag="ACHURQ_001307"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR0823134.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHURQ_001308"
     CDS             1891..3927
                     /gene="lpoA"
                     /locus_tag="ACHURQ_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR0823135.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3885..4280
                     /locus_tag="ACHURQ_001309"
     CDS             3885..4280
                     /locus_tag="ACHURQ_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR0823136.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4300..4890
                     /gene="diaA"
                     /locus_tag="ACHURQ_001310"
     CDS             4300..4890
                     /gene="diaA"
                     /locus_tag="ACHURQ_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR0823137.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4900..5475
                     /gene="dolP"
                     /locus_tag="ACHURQ_001311"
     CDS             4900..5475
                     /gene="dolP"
                     /locus_tag="ACHURQ_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR0823138.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5589..6629)
                     /locus_tag="ACHURQ_001312"
     CDS             complement(5589..6629)
                     /locus_tag="ACHURQ_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0823139.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6702..7337)
                     /locus_tag="ACHURQ_001313"
     CDS             complement(6702..7337)
                     /locus_tag="ACHURQ_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR0823140.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHURQ_001314"
     CDS             7465..7983
                     /gene="yhbO"
                     /locus_tag="ACHURQ_001314"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR0823141.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7963..8406)
                     /locus_tag="ACHURQ_001315"
     CDS             complement(7963..8406)
                     /locus_tag="ACHURQ_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR0823142.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHURQ_001316"
     CDS             8457..8759
                     /gene="yhbQ"
                     /locus_tag="ACHURQ_001316"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR0823143.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8746..9249)
                     /locus_tag="ACHURQ_001317"
     CDS             complement(8746..9249)
                     /locus_tag="ACHURQ_001317"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0823144.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9243..9767)
                     /locus_tag="ACHURQ_001318"
     CDS             complement(9243..9767)
                     /locus_tag="ACHURQ_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR0823145.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9976..10971
                     /locus_tag="ACHURQ_001319"
     CDS             9976..10971
                     /locus_tag="ACHURQ_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR0823146.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10980..11858
                     /locus_tag="ACHURQ_001320"
     CDS             10980..11858
                     /locus_tag="ACHURQ_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR0823147.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12064..13071
                     /locus_tag="ACHURQ_001321"
     CDS             12064..13071
                     /locus_tag="ACHURQ_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR0823148.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHURQ_001322"
     CDS             complement(13189..14433)
                     /gene="mtr"
                     /locus_tag="ACHURQ_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR0823149.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="ACHURQ_001323"
     CDS             complement(14587..16476)
                     /gene="deaD"
                     /locus_tag="ACHURQ_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR0823150.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="ACHURQ_001324"
     CDS             complement(16469..16549)
                     /gene="yrbN"
                     /locus_tag="ACHURQ_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR0823151.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="ACHURQ_001325"
     CDS             complement(16656..17540)
                     /gene="nlpI"
                     /locus_tag="ACHURQ_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR0823152.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="ACHURQ_001326"
     CDS             complement(17649..19784)
                     /gene="pnp"
                     /locus_tag="ACHURQ_001326"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR0823153.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="ACHURQ_001327"
     CDS             complement(20031..20300)
                     /gene="rpsO"
                     /locus_tag="ACHURQ_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR0823154.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20449..21393)
                     /gene="truB"
                     /locus_tag="ACHURQ_001328"
     CDS             complement(20449..21393)
                     /gene="truB"
                     /locus_tag="ACHURQ_001328"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR0823155.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="ACHURQ_001329"
     CDS             complement(21393..21794)
                     /gene="rbfA"
                     /locus_tag="ACHURQ_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR0823156.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21958..24630)
                     /gene="infB"
                     /locus_tag="ACHURQ_001330"
     CDS             complement(21958..24630)
                     /gene="infB"
                     /locus_tag="ACHURQ_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR0823157.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="ACHURQ_001331"
     CDS             complement(24655..26142)
                     /gene="nusA"
                     /locus_tag="ACHURQ_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR0823158.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="ACHURQ_001332"
     CDS             complement(26170..26622)
                     /gene="rimP"
                     /locus_tag="ACHURQ_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR0823159.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26829..26905)
                     /locus_tag="ACHURQ_001333"
     tRNA            complement(26829..26905)
                     /locus_tag="ACHURQ_001333"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26869..26871),aa:Met,seq:cat)
BASE COUNT         6424 a         7518 c         6809 g         6357 t
ORIGIN      
        1 acctctgcaa cgctaagtag cactaactgg caatacgcct gttcctgctc tgcgggtaag
       61 gcagttaaac ttgtctatat ggtcagcccc gtacttacca ccactggaca tcagacagga
      121 tattacaaac tcaatgacag cctggatatt aaaaccacat tacaggcaaa cgacattcca
      181 ggactcacaa ccgaccaggt tgtctctgtt aacacccgat tcacacagat aaaaagcagc
      241 accgtatatt ctgctgcaac ccaaacgggt gtttgccagg gtgacacgtc tcgttatgga
      301 cccgttaata ttggtgcaaa taccaccttt accctgtatg tcaccaagcc atttctcggc
      361 tcgatgacca ttccgaaaac ggatattgcc gtcattaaag gcgcgtgggt cgatggaatg
      421 ggaagcccgt ctacaggtga cttccatgat ttagtcaagt tatcgattca gggaaatctc
      481 accgccccac agtcgtgcaa aattaatcag ggcgatgtta ttaaggttaa ttttggattc
      541 atcaatggtc agaagtttac cacccgcaat gccatgccag acggttttac tccagtagac
      601 tttgatatca cttatgactg tggtgatact tcaaagatta aaaactcgtt gcaaatgcgc
      661 atcgacggta caactggggt agtagaccag tacaacctgg tcgccaggcg aagaagttca
      721 gacaatgtgc ccgatgtcgg tattcgtatt gaaaatctcg gcggcggagt tgcaaatatt
      781 ccttttcaga acggtatcct tcccgttgat ccttccgggc atggcaccgt caatatgcgc
      841 gcctggccag ttaatctggt cggtggtgag ctggaaacag gaaaatttca gggcaccgcc
      901 accattaccg tcatggtgcg gtaaaagaag acgttgcgtt ttgattaacg caacgcttcg
      961 gcactgttac ccctgctgct ccagcgcata cttatacagc gcatttttct tcacgccatg
     1021 aatttctgcg gccagcgccg ccgctttttt cagcggcagt tctgcctgta gcagcgccag
     1081 cgtgcgcagg gcatcggcgg gtaagtcttc ttcctgtgct ttatgacctt cgacaatcag
     1141 caccatttcg cctttgcgac ggttttcatc ttcctttacc cacgccagca gctcgccaac
     1201 gggcgcgccg tgaatggttt cccaggtttt ggtcagctca cgcgccagaa ccacgtagcg
     1261 ggattcgcct aataccgcaa cgatatcttc caggctatct aacagacggt gggtagattc
     1321 ataaaaaatc agcgtgcgcg gctccgcttc aatggctttt agcgcatcac ggcggccttt
     1381 tgatttggca ggtaaaaagc cttcgtaaca gaaacggtca gagggtaaac ccgcggcgct
     1441 taacgcagtg atagcagcac acggtccggg tagcggcacc acacgaatcc ccgcttcacg
     1501 gcaggtacgc accagatggt agccaggatc gttaattagc ggcgttccgg catcggaaac
     1561 cagcgcaatg ttttgcccct cttgcagctt cgccagcagc gtttcggctt tttgttgttc
     1621 gttatggtcg tgcagcgcaa acaaccgggc attaatccca aaatgttgca gcaataaacc
     1681 ggtgtgacga gtatcctcgg cggcaatcag atcaacggcc tgtaatacct ctaacgcacg
     1741 ctgggtgata tccgccagat tgccgattgg cgtcggtaca atgtaaagct ggccctgaga
     1801 attatccgcc gattggtgtt gtttcattgt gtcgtccgta ttgccgattt aatattgagc
     1861 attgcgtaaa aaaatatcac tggatacatt atggtaccct caacattttc tcgtttgaaa
     1921 gccgcgcgtt gtctgcctgt tgttctggca gccctgattt tcgccggttg tggcacccat
     1981 actcccgatc agtccactgc ttatatgcag ggcacggcgc aggctgattc tgccttttat
     2041 cttcagcaga tgcagcaaag ctctgatgat accaggatca actggcaatt actcgccatt
     2101 cgtgcactgg tgaaagaagg taaaaccggg caggcggttg agttgtttaa ccaactaccg
     2161 caagaactga acgattctca gcgtcgcgag aaaacactgc tggcggtaga gattaaactg
     2221 gcgcagaaag attttgctgg cgcgcaaaac ttgctggcga aaatcacacc tgccgattta
     2281 gaacaaaacc agcaagcgcg ttactggcag gcaaaaatcg atgccagcca ggggcgtcct
     2341 tccattgatt tactgcgcgc gttaattgct caggaaccgc tgctcggcgc gaaagaaaaa
     2401 aagcagaata ttgatgccac ctggcaggcg ctctcctcca tgactcagga acaggcgaat
     2461 acgctggtga tcaacgccga cgaaaatatt ctgcaaggct ggctggatct gcagcgcgtc
     2521 tggtttgata accgtaacga tcccgacatg atgaaagccg ggatcgccga ctggcaaaaa
     2581 cgttatccga acaatccggg cgcgaaaatg ctgccaacgc agttggttaa cgtaaaagcg
     2641 tttaaaccag cctcgaccaa caaaatcgcc ctgctgttgc cgctgaatgg ccaggcagcg
     2701 gtatttggtc gcaccattca gcaaggcttt gaagcggcga aaaatatcgg cactcagcca
     2761 gtggcggctc aggtagctgc cgcacctgcc gcagacgtag ctgaacaacc tcagccgcaa
     2821 accgtggatg gcgttgccag cccggcacaa gcctcggtta gcgatctgac cggtgatcag
     2881 cctgcggccc agccggtgcc tgtaagcgcc ccggcgacaa gcaccgcagc ggtaagcgca
     2941 cccgcaaatc catccgcaga gctgaaaatc tacgatacct catcacaacc acttagccag
     3001 atcttaagcc aggttcagca ggatggcgcg agtattgtgg ttggtccgtt gctgaaaaat
     3061 aacgttgaag agttgctgaa gagcaacacc ccgctgaacg tgctggcact gaaccagcca
     3121 gagaatatcg aaaaccgcgt caatatttgt tacttcgcgc tttcacctga agacgaagcg
     3181 cgcgatgcag cgcgtcatat tcgtgaccag ggtaaacaag cgccgctggt gctgatccca
     3241 cgcagttcat tgggcgatcg cgtagccaat gcgtttgcgc aagagtggca gaaactgggc
     3301 ggcggcaccg ttctgcaaca aaaatttggt tccaccagcg aattacgcgc gggtgttaac
     3361 ggcggttctg gtatcgcttt aacgggtacc ccgattactc ccagagcgac aaccgactcc
     3421 ggcatgacga ccaacaatcc aacgctgcaa accacgccaa ccgatgacca gttcaccaat
     3481 aatggcggtc gtgtcgatgc ggtgtacatt gtggcaacgc cgggtgaaat cgcttttatc
     3541 aaaccgatga tcgccatgcg taacggtagc cagagcggtg caatgctgta cgccagctcc
     3601 cgcagcgcac aaggcaccgc aggcccggat ttccgtctgg agatggaagg tttgcagtac
     3661 agcgaaatcc cgatgctggc gggcggtaat ctgccgttaa tgcagcaggc actcagcgcg
     3721 gtgaataacg attattcact ggctcgcatg tatgcgatgg gcgtcgatgc ctggtcgctg
     3781 gcaaatcatt tctcacaaat gcgccaggtt cagggttttg aaatcaacgg taataccgga
     3841 agcctgacgg caaacccgga ttgcgtgatt aacaggaagt tatcatggct acagtaccaa
     3901 caaggtcagg tagtccccgt cagttaacca ccaaacagac cggcgatgcg tgggaagcac
     3961 aagcgcgtcg ctggctggaa ggcaaaggac tgcggtttat cgccgctaac gtgaacgagc
     4021 gtggcggcga gatcgatctg ataatgcgtg aaggccggac caccgttttt gtcgaggtac
     4081 gctatcgccg ctctgcgctt tatggcggcg cggcagccag tgtgacccgc agcaaacaac
     4141 acaaattatt acagactgcc cgcttgtggc tcgcgcgtca taatgggagt tttgatactg
     4201 tggattgccg gttcgatgtg gtagccttca ccgggaatga ggttgagtgg attaaggatg
     4261 cctttaatga ccactcataa ttaaggttta aggattagcg tgcaagaaag aattaaagct
     4321 tgcttcactg aaagcattca aactcaaatt gcggcggcag aggcgcttcc ggatgccatc
     4381 tcccgtgcag ccatgacgct ggttcagtct ctgctcaatg gcaacaaaat cctctgttgt
     4441 ggtaatggaa cttccgctgc caatgcacag cattttgctg ccagcatgat caaccgtttc
     4501 gaaacggagc ggcccagctt acctgccatt gcactaaata ctgataatgt tgtcttaacg
     4561 gcgattgcca acgatcgctt acatgatgaa gtgtatgcaa aacaggtgcg ggcgctgggt
     4621 catgcgggag atgtattgtt agccatttcc acccgtggca acagccgcga tattgttaaa
     4681 gcagttgaag ccgccgttac gcgtgatatg accattgtgg cattgaccgg ctatgacggc
     4741 ggcgagcttg caggtttgtt agggccacag gatgtggaga tccgcattcc ttcgcatcgt
     4801 agtgctcgca ttcaggaaat gcatatgctg acggtaaatt gcctgtgcga tctgatcgat
     4861 aacacgcttt tccctcacca ggatgattaa ggagaataca tgaaggcatt atcgccaatc
     4921 gcagtcctta tttccgcgct gctgttgcaa ggttgtgttg ccgctgccgt agtgggtacc
     4981 gctgctgtgg gtaccaaagc cgcaactgac ccacgcagtg tcggcaccca ggtggacgat
     5041 ggtaccctgg aagtgcgcgt gaacagcgca ttgtcgaaag acgaacagat taagaaagaa
     5101 acccgtatta atgtcacggc gtatcagggc aaagtgctgc tggttgggca gtcaccaaac
     5161 gctgaacttt cggctcgcgc caaacagatt gctatgggcg tagacggtgc caacgaagtg
     5221 tataacgaga ttcgtcaggg ccagccgatt ggtctgggcg aagcatctaa cgatacgtgg
     5281 atcaccacca aagtgcgttc gcagctctta accagcgacc tggtgaaatc gtccaacgtg
     5341 aaagtgacca ccgaaaacgg tgaagtgttc ctgatggggc tggtgactga acgtgaagcg
     5401 aaagcggcgg cagatattgc cagccgggtg agcggcgtaa agcgggtaac cacagcgttt
     5461 acgtttatta aatagcagcc ccttgtaatg cctgatgcga cgcttgccgc gtcttatcag
     5521 gcctacaacg atgcaaaccg taggtcggat aaggcgttta cgccgcatcc gacatgtttt
     5581 ccctcaaatc agaataacag cgccagcccg ccgacaatca cgcctgacac tgccaccatc
     5641 gcccccgtca gccataaggc tttcgccggg aacgctttgc gcagcataat cagtgacggc
     5701 aaactcactg ccgggagcgt catcaacaat gccagcgccg gagcggttcc catacctgcc
     5761 agcatcatcg tttgtacaat cggaatttct gctgccgtgg gaatgacaaa caagcatcct
     5821 gctaccgcca tcgccaccac ccacatcagg ctgttatcga cagcaccatc ggcatggggg
     5881 aataaccaga cgcgtgcggc acccaacacc agtactgcaa ggatgtaaac cgggatcgta
     5941 ctccagaaaa gtgtccatag cgccctgccc cagcggctaa aaaaaccgct ctgtgcttcc
     6001 ggtatgtcaa tttcgaccgg tgcctgcgtt tgcggtgttt cacgcaccca tttttgcacc
     6061 agcgtcgcaa tcagcaacac catcaccagc ccggccacca gacgaatcgc cgcaaaaccc
     6121 cagccgagga caaaccccat aaacaccagc gtcgccgggt ttaacaccgg attgcccatc
     6181 cagaatgcca gcgcaccgcc catcgatacc tgttggcgac gcattcccgc cgcgaccgga
     6241 gccgcacagc aggtacacat catgcccggc agcgaaaaca gcgttcccag cagcgtgccg
     6301 cgaaagcgcg attgtccaag cgtacgcaac aaccagtcac gcgggatcag cacctgaatc
     6361 aacgaaccga ggatcacccc cagcaccgcc gctttccata ccgcgaggaa atagatcatc
     6421 gcgtaatcca acgccgcctg ccacggatta gcatccgcct gcgcaaggat agatttaccg
     6481 atactgtggg tttcggcagc agtaaacgct ttgccgtagt aaggttgcca tttcacatac
     6541 cagagaccgg caatgacaac gagaaagaaa agcgcgggtt tccaccactg aatgggcgtt
     6601 gccgcctgag atgaagactg accagtcata gcattcccca ggaataagtt atgataattg
     6661 agcgcgtgaa tattacgctc actatcaatt cttgggaata attattcagc tcttttgcgt
     6721 aattctgaag agcttaaaat agtcacgcct tcatgctcag gccgcatcgc ctctgccagc
     6781 atgacccgcg ccacgtctct cgcatcaatg gatttccagt taccgggcag taaacgaaac
     6841 agcggcgcaa aaagcgtttc gttcatccgc tgtttgctac gatcgcccag caacatcgac
     6901 gggcgagcaa tggtcaattt cggccagttc tgggcaatta atgcttcttc catctcccct
     6961 ttgacgcggt tatagaaaaa cggcgagtgg gcattggcac ccatagcact gaccaccagc
     7021 atatgctgcg cgcccagtcg ccgcccggtt aatgcggtat ccactaccag cgtgtaatcg
     7081 gcatgaataa acgcctcttt gctccccgct tctcgccgcg tggtgccgag acaacaaaac
     7141 acaatgtcga tgggttcggt gacctgcgcc aacgcgtcac tcagttgcgg atcatgggga
     7201 ttaaacaccc cgggcatatc gcccaacgga cgtcgcgtcg gcgcggctat ggcgttaact
     7261 ttcggttcgt taatcaacat ccgcagcagg tgaccgccca ccagccccgt tgcgcctgta
     7321 atcagtacct gactcatctt cgctccttta cagaattgtc tgccttgcgc tccacggctc
     7381 atgcaccagg cttaatagac catgaggtaa ttatccccga ttgtgtgaaa ttcgcctcat
     7441 ccaatgcaac aacgcggagg aagcatgagt aagaaaattg ccgttttaat cagtgatgaa
     7501 tttgaggatt cagaatttac ttcacccgca gacgagttcc gtaaagccgg acacgaagtg
     7561 attaccattg aaaaacaagc gggtaaaacg gtgaaaggca aaaaaggaga agccagcgtg
     7621 accatcgata aatccatcga tgaagtgacg ccagcggagt ttgatgccct gctgctaccg
     7681 ggtggtcatt caccggatta tctgcgtggc gacaaccgtt ttgtcacctt tacccgtgat
     7741 tttgtgaata gcggcaaacc tgttttcgcc atctgtcacg gtccgcagct gctgatcagc
     7801 gccgatgtga ttcgggggcg caaactgacc gcagttaaac cgatcattat tgatgttaaa
     7861 aatgcgggcg cggaatttta cgatcaggaa gtcgtggttg ataaagatca gctggttacc
     7921 agccggacac cggacgatct gccagcgttt aaccgcgagg cgttacgcct gctcggtgcc
     7981 tgagtcgcgt agccaaatca tttttttacc aaagcccagc gtgttgtcag tgaatttgat
     8041 ttcatcgagc cggatttccc acaccggggc cgacagcatt ctggcaaccg gaaagcgacg
     8101 attgtacgcc tggcgcgcga ggtcgctttc ctcaccttcc agcctgcgga tctcaccttt
     8161 aaactgcacg ccgcgaatta acgctaccgt tttcggctga ccgtttaccg ttccggcaac
     8221 cgcagcctgc ggcccactca tctgcgcgtg gcgcgttttt tcttccgtca aaatgtagaa
     8281 ggcgactttc tgcgcatcaa acagataaaa agcattagcg caccacagtt ccccttcctg
     8341 ctgcacacac caggtgacaa catgttgttt tgccaaccaa cggctgatgg tgatgagtgt
     8401 ttccattgct gctctctttt atactgtggg cctgacctta acatgcgacg cagacgatga
     8461 caccctggtt tctttacttg atccgtaccg ccgacaataa gctttatacc gggatcacca
     8521 cggatgtcga acgccgctat cagcagcacc aaagcggcaa aggggcgaaa gcactgcgcg
     8581 ggaaaggaga actgacgctg gcgttttctg cgccagtggg cgatcgttcg ctggcgttac
     8641 gggcggaata tcgcgttaag caactgacca aacggcaaaa agagcgtctg gtagcggaag
     8701 gcgcagggtt tgcagagctg ttaagcagtc tgcaaacccc ggagattaaa agcgattgaa
     8761 atgctcgtga tactcaacca gaccggttac gccattcagc gcgtcatccg ctaaacgatg
     8821 tacctggaag gcgctttcag tgcccggcca gcggcaacgc agatcgtgat gcgccgccag
     8881 ttcaaagccg aaacgactgt acagcgccgg atcgcccagc gtcaccactg cggcatagcc
     8941 gaactcatta agcgaatcga gtccttcata gaccagttgg cgtgccagcc cctgtccacg
     9001 gtatttttca tcaaccgcca acggtgccat gccgacccat tgcaggtctt cgccctgcac
     9061 atcaaccgga ctaaatgcca catagccaat gacctgacct tcgtcatctg tcgccaccag
     9121 ccccagcgtc agaaagccat cttcacgcag atcgtgaacc agcttcgctt ccgcatcact
     9181 ttcgaatgag cgacgcagca gggcatcaat acccggcgca tcaatgggaa tttctactcg
     9241 aattagcatg gttcacctac cgatgtctgt ttggtttcag gcgcggtttt cattcccgcc
     9301 tcaacaaaat ccgccagttg cagcagcatc atgcgtaagg ctttcggcat ttgctccagt
     9361 tcaatggcgt ccatcaggtt tttcacatac agccccagct ccgtatcgcc ttcaatcacc
     9421 agccgacgct ggaagaagag cgtatccgga tcttgtttac gcgccgcaat catcagcaga
     9481 tcgctggcgt cggcactaaa actcacatca gcttgcgcgt tctggctgac caccagtttg
     9541 ccattcacca ccgaggtaaa ccattgcagg tcaatatcac gcacatgaat acttaaccag
     9601 cggccttcaa gaaactccag ctcaccatca tccagcgcct gacggaattg ccagcttaag
     9661 acctgctcaa gaacctggcg ttttagcgca aatggcgtca gttttaccgg tacactcaac
     9721 agagatggcc ccaaatgcac aatacgggaa cgcagtttat ccaacacgag ttttactccc
     9781 tgtttcaaca atcatcctat tttgccatat cagaaaaata acatagcggt ataaatcaac
     9841 aattccatat gaaattgctg ctaccaccaa tacaacttta actgccttaa atcaaaaatt
     9901 gtcgcagcaa ggttaactaa aatcccagtt cgttaacatt tttgcgtttt gaaagcgcaa
     9961 ccttcaggaa aaattatgga gctgctctgc cctgccggaa atctcccggc gcttaaggcg
    10021 gccatcgaaa acggcgcaga tgctgtttat atcgggctaa aagatgatac taatgcccgt
    10081 cacttcgccg gccttaactt taccgagaaa aaattgcagg aagcggtgag ttttgtccat
    10141 caacatcgcc gcaaacttca catcgcgatt aacacttttg cgcatccgga cggttacgcc
    10201 cgttggcagc gcgccgtgga tatggcggcg cagctgggtg ccgacgcgct gatcctcgcc
    10261 gacctcgcca tgctggagta cgccgccgag cgttacccgc atattgagcg tcatgtgtca
    10321 gtgcaggctt cggcgaccaa tgaagaggcg attaactttt atcatcgcca ttttgatgtt
    10381 gcccgcgtgg tgctgccgcg cgtgttgtcg attcatcagg tgaaacagct ggcacgggtc
    10441 acacctgtcc cgctggaagt ctttgctttt ggcagcctgt gcattatgtc ggaaggtcgt
    10501 tgctatctgt cgtcgtatct gacgggtgag tcgcccaaca ccgtgggcgc gtgttctccg
    10561 gcccgtttcg tgcgctggca acaaacgccg caggggctgg aatcccgcct gaacgaagtg
    10621 ctgatcgacc gttatcagga tggcgaaaac gcaggttatc cgacgctgtg taaagggcgt
    10681 tatctggtgg acggcgagcg ctatcacgcg ctggaagaac caaccagtct caataccctg
    10741 gaactgctgc cggagttaat ggcggcgaat attgcttcgg tgaaaattga aggccgccag
    10801 cgtagcccgg cgtatgtcag ccaggtggcg aaagtctggc gtcaggctat cgaccgttgt
    10861 aaggccgatc cgcaaaactt cgtaccgcaa agcgcgtgga tggagacgct cgggtcgatg
    10921 tccgaaggca cgcagaccac ccttggcgcg tatcaccgta aatggcagtg agaagagcaa
    10981 tgaaatattc cttagggcca gtgctgtggt actggccaaa agaggcgctg gaagaatttt
    11041 atcagcaggc cgccaccagc agcgccgacg tgatttatct tggtgaagcg gtatgcagca
    11101 agcgccgggc aaccaaagtt ggcgactggc tggagatggc aaaatcgctc gccggaagcg
    11161 ggaagcagat agtgctctcc acgctggcgc tggtgcaggc atcctctgaa ctgggcgaac
    11221 tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc cagcgatctc ggcgtggtga
    11281 atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca cgcgctgaac tgctacaacg
    11341 cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg ctggtgtatg ccggtggagc
    11401 tttcccgcga ctggctggtg aatctgctta atcagtgcga taagttgggg attcgcaacc
    11461 agtttgaagt ggaagtcctg agctacggtc atctaccgct ggcctactcc gcccgctgct
    11521 ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga aacctgctgc attaagtatc
    11581 cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt gtttgtactc aatggcattc
    11641 agaccatgag cggctacgtt tacaacctcg gtaacgagct gacatccatg cagggattgg
    11701 ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt cgcgatgctc gacgccttcc
    11761 gcgctaatga aaatggcgct gcgccactgc cgttgacggc aaacagcgac tgtaacggct
    11821 actggcggcg gctggctggg ctggagctgc aagcctaagt aaatatgctc gtcatacttc
    11881 aagttgcatg tgctgcatct gcattcgttc accccagtca cttacttatg taagctcctg
    11941 gggattcact ctcttgtcgc cttcctgcaa ctcgaattat ttagagcaca gctcactttg
    12001 ttaacaactt taactactct ttaatgcagt attaaagatt aatcggtaac aaagtgagct
    12061 gttatgactg ataaaaccat tgcgttttcg ctactcgatc tggcccccat tcccgaaggt
    12121 tcttcagcgc gagaagcatt ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc
    12181 ggctatcatc gctactggct ggcagaacac cacaatatga ctggcattgc cagtgctgcc
    12241 acgtcggtat tgattggcta tctggcggcg aataccacca cgctgcatct ggggtctggc
    12301 ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg aacagttcgg cacgctcaat
    12361 acactctatc cggggcgaat cgatttgggg ctgggtcgtg cgccaggtag tgaccaacgg
    12421 acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg ataatttccc ccgcgatgtc
    12481 gcggagctgg tggactggtt tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca
    12541 ggctatggcg agaaaatccc cgtgtggttg ttaggctcca gcctttacag cgcgcaactg
    12601 gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact tcgcgccgga tatgttgttc
    12661 caggcgctgc atctttatcg cagcaacttc aaaccgtcgg cacgactgga aaaaccatac
    12721 gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc gcgacgctga atttctattt
    12781 acctcaatgc agcaagcctt tgtgaagctg cgccgtggcg aaaccggaca actgccgccg
    12841 ccgattcaaa atatggatca gttctggtca ccgtctgagc agtatggcgt gcagcaggcg
    12901 ctgagtatgt cgttggtggg tgataaagcg aaagtgcgtc acggcttgca gtcgatcctg
    12961 cgcgaaaccg acgccgatga aattatggtt aacgggcaga ttttcgacca ccaggcgcgc
    13021 ctgcattcgt ttgaactggc gatggatgtg aaggaagagt tgttgggata gtgtgtctta
    13081 acgcgggaag ccttatccga gctggcaacg ctgtcctacg tagacctgat aagcgaagcg
    13141 catcaggcat tgtgtaggca gcagaaatgt cggataaggc accgctgatt actgatacac
    13201 cggcagcaaa ttaaagctcg ataagatatg gaccagtgcg ttgccaacgc caaacaccag
    13261 aatcagcgta atcatcggct tgccgcccca gacacggaat ttcgggctgc caaagcgttt
    13321 acgcgatgcg cgggctaaca gcgccggaac aattgccgcc cagatggtag ctgctaaacc
    13381 agcataacca atggcgtaca ggaatccgtt cgggaacagt agaccaccca caacaggcgg
    13441 ggcaaaggtc agcaatgccg ttttcaagcg ccccagggct gagtcatcga aaccaaacag
    13501 atctgccaga tagtcaaaca aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa
    13561 gtttgagaac acgaccagca gaaggtccag gctacggctg ttcagcacgc cgcttaatgc
    13621 ctgtaccagc acatcaatat taccgccctt ctctgcaata ccgataaact ccggacgcgg
    13681 gatgttaccc atcgtcgcca gcaaccagat ggtatacagc gccagtgcca tcagcgtacc
    13741 gtacaccagg catttcacga tggttttcgg atctttgccg taatacttca tcaggctcgg
    13801 cacgttaccg tgataaccaa acgatgccag acagaacggc agggtcatca gcaaatacgg
    13861 cgcataagat gcattgcttt cggcgacgtt gaacaatgtc gcaggctgca catgccccag
    13921 taggctaccg aaggtgagga agaaggtaat gactttcgcc cccagtacaa tcgccgtcat
    13981 gcggctgacg gccttagtac tcaaccacac cacaaacgct accagcagtg caaaaccaaa
    14041 acccgccgcc cgtgccggga cgtttagcga catctctgcg aaggtgtgat gcagaattga
    14101 accactggca gaaatatagg cataggtcag gatatagagc acaaaggcaa tggaaatacc
    14161 gttgaccacg ttccagcctt tgcccagcaa atctttggtg atggtgtcaa aactcgaacc
    14221 gattcggtaa ttcaggttag cttccagaat catcaagccg gaatgcagca tacagaacca
    14281 ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc ccggacatga ccactggcag
    14341 agaaaacatc cctgcgccaa taatggtgcc gccgataatc accacgccgc caagcagcga
    14401 cggtgacgtt tgggtggtgg ttagtgttgc catgagagct tctctccagt gaaaaatagt
    14461 gcgactgcgt tgttatgcat tgcactgtac cagtacacga gtacaaaaga cagaaaaaaa
    14521 gccccgatgg taaaaatcgg ggctgtatat attattttac agattgtgtt cgctgttcag
    14581 cgatgattac gcatcaccac cgaaacgacg acgaccggta gagtcatcac gacgcggagc
    14641 gcggccttca cgacgttcgc cgctaaaacg acgaccatca ccacggccac cttcacggcg
    14701 ttcaccgctg aagttacgac cgccttcacg acgttcgcca ccgaaaccac gaccaccgcc
    14761 acgacgctca ccgccagtat gcggctgtgc atcgcccagt aactgcatgt tcatcggctt
    14821 gttgagaatg cgagtgcgcg taaagtgttg cagcacttca cccggcatac ctttcggcag
    14881 ttcgatggtg gagtgagaag caaacagctt gatgttacca atgtaacggc tactgatgtc
    14941 gccttcgtta gcaatcgcac caacgatatg acgaacttca acaccatcat cgcggcccac
    15001 ttcaatgcgg tacagctgca tatcgccaac atcacgacgt tcacgacgcg gacgatcttc
    15061 acggtcacca cgcgggccac ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg
    15121 gaattcacgt ttcggacgca tcggcgcatc tggcggtacg atcagagtac gttcaccctg
    15181 tgccattttc agcagtgccg cagccagagt ttcgagatcc agctcttcac cttctgcagt
    15241 cggctgaatt ttgctcagca gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg
    15301 ctgtacttta gcggcgaatt tttccagacg gcgtttgccc agcagttctg cgttcggcag
    15361 ttctacttcc ggaatagtca gcttcatagt acgttcaatg ttgcgcagca gacgacgctc
    15421 gcggttctca acgaacagca gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat
    15481 acggtgaacg taagactcag aatccatcgg gatatcgtag ttaactacca ggctgatacg
    15541 ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc aggatgtcca gacgaccatc
    15601 tttcaggcgt tccagtgtct gttcacgcag cgcctggttc atgtcaccgt tcagcgcggc
    15661 gctgttgtag ccgttacgct caagagcttc agccacttcc agagtcgcgt ttttggtacg
    15721 aacgaagata atcgccgcat caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt
    15781 tttgcgcata ccccagacag tccagtagct ctggctgatg tcaggacggg tagtcacgct
    15841 ggactgaatg cgcacttcct gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc
    15901 ttccggcatg gttgcagaga acagagcggt ctgatgacct tccgggatct gcgccataat
    15961 ggtttcaacg tcttcgatga agcccatgcg cagcatttcg tcagcttcat ccagaaccag
    16021 accgctcagt ttagagaggt ccagagtgcc acgtttcagg tggtccagca gacggcccgg
    16081 agtaccgaca acgatctgcg gcccctgacg cagggcgcgt aattgcacgt cataacgctg
    16141 gccgccgtac agagcaacca catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc
    16201 ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc accagaatct gtggtgcttt
    16261 cagctcagga tcaagattct gcaacagagg taaagagaat gctgcagttt ttccgctccc
    16321 cgtctgggcc atacccagaa cgtcgcggcc attcagcaga tgtggaatac actctgcctg
    16381 aattggagat ggtttttcgt aacccagatc gttaagggct tcaaggatag gagccttcag
    16441 gcccagatct gcaaaagtgg tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa
    16501 tggcggccag tctacataac tcatcatgaa attgatcagc aattttcatt gaaaagtgtg
    16561 aaccggctca aagtaggtgt attaacgaac aacaacgccc tcacccgtta aggtgatggc
    16621 aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt gctggtccga ttctgccagg
    16681 tcatcttggt cctggcccag gagcgataat tccaacaatg cgtatcggtg ctcaacaaag
    16741 ttatgaacgt tgttggcaac cgccagtttg aacagtgccg tggcgctgtc caaatccccc
    16801 agacttaggt agtacttacc taaatagaag ttggtttcac tgagatgctc agcgagcgag
    16861 gtgttatccg ttgcgtccgc cttgagcctt tccattaacg tttgttcgct aatgttgccc
    16921 aggtagaact cgacaatgtt ccatccccac tgttccttat ccgatttttc gaagtgctgt
    16981 ttcaacactt ctttagcctg cttctcatcg agcttctgct cggcgagata aagccacaga
    17041 ctacggaaag gatcattggg atcgtcttga taaaacgcca gcagatcatc ttgcgctaac
    17101 ttgtcacgac cgccgtaata taatgcgatc ccgcgattca agtgcgcgta gttgtaagtt
    17161 ggatcaagct caagtacaga atcaaacgct tcataggcag catcaaaatt gcctgcctgc
    17221 gttaaatata tgcctaagta attgaatact tcaggcatat ccggtcggat tgccagcgct
    17281 tgcgaaaaat cgttacgcgc taatgccctc agaccgagac tatcatacaa cactccgcgc
    17341 tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc gactggcaag gatttgttcc
    17401 atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca atggtaccgc gaggacttca
    17461 cttttacgcc aggaagtatt actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac
    17521 cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac ggttgaatga acgtcctgtt
    17581 cccggttgct aacaaggcgt cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa
    17641 tggcaacctt actcgccctg ttcagcagcc ggagcttccg gtgctgcagc aggttgagac
    17701 tgctcagtcg cttctttaat gctcagacgg atacggccct ggcgatcaac ttctagaact
    17761 ttcaccggta cttcctgacc catctgcagg taatcggtca ctttctcaac gcgtttgtca
    17821 gcgatttgag agatgtggac cagaccttct ttaccgccgc cgatggcaac aaatgcgcca
    17881 aagtcaacga tacgggtcac tttaccagtg tagacgcggc ccacttcgat ttctgcagtg
    17941 atctcttcga tacgacgaat agcatgtttc gctttctcgc cgtcggtcgc tgcgatcttc
    18001 acagtaccgt catcttcgat ttcgatggta gtgccagttt cttcggtcag ggcacggata
    18061 acagagccgc ctttaccgat aacgtctttg atcttatccg ggttgatctt gatggtatgg
    18121 atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg
    18181 cccaggatat gcagacgcgc acctttagcc tggttcagcg caacctgcat gatctctttg
    18241 gtgatacctt caattttgat atccatctgc agtgcagaga taccgtcgcg ggaacctgca
    18301 actttgaagt ccatatcgcc caggtgatct tcgtcgccca aaatgtcaga cagtacaacg
    18361 tagttgtcgc cttctttcac cagacccatt gcgatacccg caacggcagc tttgattggc
    18421 acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg aagccataga agaagaaccg
    18481 ttggattcgg tgatttcaga cactacacgt acggtgtacg ggaatttgtc catatccggc
    18541 atgactgcca gcacgccgcg cttcgccaga cgaccgtgac caatttcacg acgcttcgga
    18601 gaaccgacca tgccggtttc gcctacggag tacggaggga agttgtagtg gaacaggaag
    18661 gtatcggtac gttcgcccat cagttcatca agaacctgcg cgtcacgagc ggtacccagc
    18721 gttgcagtaa ccagtgcctg cgtttcaccg cgggtgaaca gcgcagaacc gtgagtacgc
    18781 ggcagcacgc cagtacgcac atccagacca cggatcatat ctttttcacg accgtcgata
    18841 cgcggttcgc ctgccagtac gcggctacga acaacgtttt tctcgatagc gtgcagaatt
    18901 tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca
    18961 gatttgatga catcaacctg cgcataacgc tcttgtttgt cggtgatgcg gtaagcatca
    19021 ctcagacgag cttcagccag tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc
    19081 ggctgccagt cccaacgcgg tttaccggct tctttcacta gttcattgat gttctgaata
    19141 acaacctgct gttgttcatg accgaacact actgcgccca gcatctgatc ttcgctcagc
    19201 agttcagctt cagattcaac catcagtaca gcggcttcag taccggcaac aaccagatcc
    19261 agtttgctct ctttcagctc gtcctgagtc gggttcagta cgtactggtc attgatgtaa
    19321 cctacgcggg cagcaccaat cgggccattg aacggaatac cagacagaga cagtgctgcg
    19381 gaagcaccaa tcatcgcgac gatatccggg ttaacctgcg ggttaacaga aaccacggtg
    19441 gcgataacct gaacttcgtt gacgaagcct tccgggaaca gcgggcgaat cgggcggtca
    19501 atcagacgcg cgatcagggt ttcgccttcg cttggacgac cttcacgacg gaagaagcta
    19561 cccgggatac gaccagcagc gtaggtacgc tcctgatagt taacggtcag tgggaagaag
    19621 tcctgacctg gtttggcttt tttctgtcca acaacggtaa cgaataccgc ggtgtcatcc
    19681 atgctaacca taacagcggc agtagcctga cgagccatca tgccggtttc cagagtcacg
    19741 gtgtgttggc cgtactggaa tttacgaacg atcggattaa gcaatgtaat atcctttctc
    19801 tttcttagac agtaccttac ggcactggtg ttaatacccg atcttctgcg catcctcgcg
    19861 actaatgaca accctaaccc agctctatgt gggtaaagcc tctcattagc cgcgcgaacc
    19921 tctgcaacgg aagatcattc atagcaacaa tacattagtt tccagtgaat tgctgccgtc
    19981 agcttgaaaa aaggggccac tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc
    20041 agacccaggc gctcgatgag ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg
    20101 agcagtttac gacgctgaga aaccatgcgc agcagaccac gacggctgtg gtgatctttt
    20161 ttgtgctctg caaagtggcc ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga
    20221 acttcggtag aaccggtgtc gtttgcgtca cgaccaaact cagaaacgat tttagctgtt
    20281 gcttcagtac ttagagacat tttaaaactc caaagtatat agaatgaaag gacgccgatc
    20341 tctaattcag cgatcccagt gtacgttacg caaagtgtta aacaatttac gcgacgttaa
    20401 gcggcagtat tctactcgta gcgacctgtt atcgcaagac ggttaacatt acgccgggta
    20461 ttcaaccacc aggcgacgag gcgcgacgcg gccttcatcg tcaatttcgc ccataccgat
    20521 aaacttgccg ttctcacctt ctgtaacgcg aaccagtcct tccagcggcg caccggatgt
    20581 acgaactggg ttaccatttt tgaagtaaac agaagacgtt aacggaagat tcaccaccgg
    20641 gtagtccgaa gctggactgt ccattggcat cagtaatgga tcaagtaact ccgcagccgg
    20701 aatatcctgc tgttcggctt gttcgaccag ttcacgcaga tgttccaggg tcaccatccg
    20761 ttcaaccgga tatttactta ccgccagacg acgcaggtaa ataacatgcg cgccacaacc
    20821 gagtttttca cccaggtcat caatgatggt gcggatataa gtgccttttg agcagtgaat
    20881 ttccagctcc agctcattgc cttcgtggcg aataaacagc aattcataaa cggtaatcgg
    20941 gcgcgcttca cgcggtactt caatgccctg acgcgcatat tcgtacagtt ttttaccctg
    21001 atatttcagt gctgaataca tcgaagggat ctgttcgata tcgccacgga aagtatccag
    21061 cgccgccgcc agttgctctg cgctaaaggt taccggacgt tcttcaacga tttgtccgtc
    21121 ggcatcagaa gtatcggtac gctgtccaag acgcgcaatg acccgatagc gtttgtcgga
    21181 gtccagcaga tactgggaaa acttcgtcgc ttccccgagg caaattggca acatgcctgt
    21241 tgccagcggg tccagcgcac cggtatgccc ggcacggttg gcgttataaa tccgtttcac
    21301 tttttgcagc gcatcattgc tggacatacc ctgcggttta tccagcaaca aaacgccgtt
    21361 aatgtcgcga ccgcgacgac gaggacgact cattagtcct ccttgctgtc gtccgggtta
    21421 acacgacgtt cttcgtcatg tttgaccacg ctggtcacca ggtttgacat gcgcatccct
    21481 tcaaccagag agttgtcgta gaagaaggtc agttccggca cgatacgcag gcgcatcgct
    21541 ttccccagca ggctgcggat gaaaccagaa gcttcttgca acgctttgat gcccgcttta
    21601 accgcgtctt catctttgtc gttgaggaac gtcacatata ctttggcata cgccaggtcg
    21661 cgagacattt cgacaccgga aacggtggtc atcatgccca ggcgaggatc tttaatttca
    21721 cgctgcagga tgagagcgat ctctttttgc atttcctgcg ctacgcgctg cgggcgacca
    21781 aattctttcg ccataataaa ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa
    21841 ttaatttcag gtggaagggc tgttcacgtt ggcctgataa gacgcgccag cgtcacatca
    21901 ggcaatccat gccggatgcg gcgtaaacgc cttatcccgc atggaaccct aaaaacctta
    21961 agcgatggta cgttggatct caatgatttc gaatacttcg atcacatcgc cagtgcggac
    22021 gtcgttgtag ttcttaacgc cgataccaca ttccataccg ttacggactt cgttaacgtc
    22081 atctttgaag cggcgcaggg actccagctc gccttcgtag ataaccacgt tgtcacgcag
    22141 aacgcggatc gggttgtgac gtttaaccac accttcggta accatacagc ctgcgatggc
    22201 accaaatttc ggcgatttga acacgtcacg aacttccgcc agaccgataa tctgctgttt
    22261 cagttccgga gacagcatac cgctcatcgc cgctttcact tcgtcaatca ggttatagat
    22321 gacggagtag taacgcagat ccaagctttc cgcttcaatc actttacgtg cagaggcatc
    22381 agcacgtacg ttgaagccaa ccaggatggc gttggacgcc gcagccaggg tggcgtcggt
    22441 ttcggtgata ccacctacgc cagaaccgat gatcttcact ttaacttcgt cagtagacag
    22501 tttcagcaag gagtcggaga tcgcttccac agaaccctgt acgtctgcct tcaggacgat
    22561 gttcacttcg tgaacttcgc cttcggtcat gttggcgaac atgttctcga gtttagattt
    22621 ctgctgacgc gccagtttaa cttcgcggaa tttaccctga cgatacagtg caacttcacg
    22681 cgctttcttc tcgtcacgta caacggtaac ttcatcaccc gcagccggta cgccggacag
    22741 gccgaggatt tccaccggaa tggacggacc cgcttccagc acttcctgac ccagttcgtt
    22801 acgcatcgca cgaacacgac cgtattcgaa gccacacaga acgatatcgc ccttgtgcag
    22861 agtaccttca cgtaccagaa cggtagcaac cggaccacga cctttatcga ggaaggattc
    22921 gataaccgca ccgctcgcca tacctttacg taccgctttc agctccagaa cttccgcctg
    22981 cagcaggata gcgtccagca gttcatcgat gccggtaccc gctttcgcag atacgtgtac
    23041 gaactggctt tcaccgcccc actcttccgg caggatgcca tactgggaga gttcgttctt
    23101 aacacgatcc ggatcggctt ctggtttatc aatcttgttc actgcaacca ccaccggtac
    23161 ctgcgccgct ttcgcgtgct ggattgcttc gatggtctgc ggcatcacgc cgtcgtcggc
    23221 agcaacaacc aggactacga tgtccgttgc ctgcgcacca cgtgcacgca ttgaagtaaa
    23281 cgcggcgtgc cccggggtgt ccaggaaggt gatcatgccg ttttcagttt caacgtggta
    23341 tgcaccaatg tgctgggtaa tgccgcccgc ttcgccagag gcaactttcg ttgaacgaat
    23401 gtagtccagc agagaggttt taccgtggtc aacgtgaccc atgatggtca caaccggcgc
    23461 gcgcggttca gccgcagcac ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt
    23521 ttcacgacgc aggataactt tgtggcccat ctcttcagca accagctgtg cggtttcctg
    23581 atcgataacc tggttgatgg ttgccattgc gcccagtttc atcatcgctt tgatgacctg
    23641 agagccttta accgccatct tgttcgccag ttcgccaacg gtgatggttt cgccgatcac
    23701 aacgtcacgg ttaacggcct gagctggctt ctggaagcct tgctgcaggg aagaaccttt
    23761 acgttttccg cctttaccgc cacgtactgc tgcgcgtgct tcttcacgat cagcttttga
    23821 ttcagcgtgt ttgttgcctt tcttcggacg cgctgctttc gcgttacgac cacggccacg
    23881 accgccttcg acttcacgat cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt
    23941 gacgtgataa tcgctggaat cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc
    24001 cgccatacga cgtgcttctt cagcaacgcg acgtgcttct tcttcgagtt tacgatgcgc
    24061 ttcttcttcg gctttacgct tgagctctgc agcttcctgc tcacggcggg ctttttcagc
    24121 ctgggcgttt ttagtcatat cgtcttgttg attgctcact ttgtcttttt ccgcagcttc
    24181 acgtttcgct tgttcagcag cttcacgctt agcttgttct gcagcctcac gttcagcttt
    24241 ttgttgcgcc tcgcgtttag ccgattcttc tgcctcacga cgggcttgct cttccgcttc
    24301 acgctgcgct tgctcttccg ctgcaaggcg ttcagcctct tgcggatcgc gtttcacaaa
    24361 ggtgcgtttc ttgcggactt cgatttgtac cgatttgctt tttccaccgg taccaggaat
    24421 gttaagggtg ctgcgtgttt tacgttgcag cgtcaatttg tccgggcctg aatttttctg
    24481 attcaggtgg tcaatcaaag tctgtttctc ttgtgcagac acagagtcgt cagcagactt
    24541 ccggatacct gcatcagcaa attgctgtac caggcgttcc acggaggtct gtcgctctgc
    24601 ggccagcgtt ttaatcgtta catctgtcat gctgttcctt cctgctacag tttattacgc
    24661 ttcgtcaccg aaccagcaaa tattacgggc agccataatc agtgctccgg ccttttcgtc
    24721 ggtcaaccct tcgatatcag ccagatcatc aatgccctgt tcggcgagat cttccagcgt
    24781 acaaacgcca cgggcggcca gtttgaatgc caaatcacga tctacccctt caaggttcag
    24841 cagatcgtca gccggtttgt tatcaccgag gctttcttcc tgggcctgtg caatggtggc
    24901 cagtgcattt ttagcacgct cgcgcagtgc ttcaacggtc ggctcatcaa ggccttcgat
    24961 ttccaacagc tctttcatcg gcacataggc cagttcttcc agcgtcgaga agccttcttc
    25021 taccagaaca gtcgcgaagt cttcgtcgat gtcgagatat ttggtgaagg tgtcgatcgc
    25081 tgcgtgcgct tccgcctgat gcttagcctg caggtcgtca acggtcatca cgttgagttc
    25141 ccaaccgctc agctgcgaag ccagacgcac gttctgaccg ttacggccaa tcgcctgcgc
    25201 caggttaccg gcttcaacgg cgatatccat ggtgtgttta tcttcatcca ccacgataga
    25261 agcaacgtct gccggtgcca ttgcgttaat cacgaactgc gccgggttat catcccacag
    25321 gacgatatcg atacgctcgc cacccagttc agtagacacc gcctgaacac gcgcgccacg
    25381 catacctacg caagcaccta ccggatcgat acgtttatcg ttggttttca ccgcgatttt
    25441 cgcacgagaa cccggatcgc gagccgctgc tttaatttca atcacttctt cgccgatttc
    25501 tggcacttca atacggaaca gttcgatcag catttccggc ttggaacgag tgacgaacag
    25561 ttgcgcgcca cgcgcttccg ggcgaacgga ataaagcacg ccacgaacgc ggtcgccagg
    25621 gcggaagttt tcacgcggca gcatatcttc acgcaggatc acggcttcag cgttgttgcc
    25681 cagatccaga gagatgttgt cgcggtttac ttttttcacc acgccggtga tgatttcacc
    25741 ttcgtgttca cggaactgat caaccaccat cgcacgttcg gcttcacgca ctttctgcac
    25801 gataacctgt tttgccgtct gggtagtgat acggtcaaag gtaacagact caatctgatc
    25861 ttcaacgtaa tcgcccaggt tcaggctttc atcttcataa cgtgcggctt caagggtgat
    25921 ttccttggtc ggctgggtga cttcatcaac aactaaccag cgacggaagg tgtcaaaatc
    25981 accgcttttg cgatcgatct gtacgcggac gtcgatctct tgttcatatt ttttctttgt
    26041 tgctgtcgcc agcgcgcttt ccaatgcttc gaaaatcttc tcgcgaggta gcgccttttc
    26101 attggatacg gcttcaacta cagccaaaat ttctttgttc atcgcgggct tttcacctca
    26161 tccagactat taaaagtggg gaaccaggtt cgccttctgg atattactca gcgcgaacac
    26221 ttcatcttta ccttcgacgg taactgtgat catttcaccg tctaccgctt tgataacgcc
    26281 ctgccattta cgacggtttt gtaccgccat acggagaacc agagtcacct cttctccgac
    26341 aaaacgggcg tagtgttcag ccgtgaacag tgggcgatcg agacccggtg aggagacttc
    26401 caggttataa gcaacggtga tgggatcttc aacatccagc acagcactta cctggtggct
    26461 cacatcagca caatcatcaa cattgatgcc atcttcacta tcaatataga tgcgcagtgt
    26521 ggatgtgcga ccgcgaataa attcgatgcc aaccagttca aaacccaggg cctcaactgg
    26581 cgcagtaatc atctctgtta atttttgctc taatgtggac aagcccaccc ccaagacata
    26641 aaaaaagggc ctaaagccca gttattctgt agtcagataa caaaaaaccc cgataaatcg
    26701 gggctttata taactgaacc ctataaccgc aactgcggtc tggagcactt tccagaagga
    26761 ttttttcaaa tcccactacg aaggccgaag tcttcacagt atatttgaaa aaggactcta
    26821 agggaaagtg gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg
    26881 agctaccagg ctgctccacc ccgcgcctga aacgtggcaa attctactcg ttttgggtaa
    26941 aaaatgcaaa tactgctggg atttggtgta ccgagacggg acgtaaaatc tgcaggcatt
    27001 atagtgatcc acgccacatt ttgtcaacgt ttattgctaa tcatgtgaat gaatatccag
    27061 ttcactttca tttgttgaat acttttgcct tctcctgctc ccccttaa
//
LOCUS       DAZOZY010000036        26909 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZOZY010000036 DAZOZY010000000
VERSION     DAZOZY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26909
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.036"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHURQ_001334"
     CDS             complement(84..806)
                     /gene="baeR"
                     /locus_tag="ACHURQ_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR0823160.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHURQ_001335"
     CDS             complement(803..2206)
                     /gene="baeS"
                     /locus_tag="ACHURQ_001335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR0823161.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(2203..3618)
                     /locus_tag="ACHURQ_001336"
     CDS             complement(2203..3618)
                     /locus_tag="ACHURQ_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0823162.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHURQ_001337"
     CDS             complement(3619..6696)
                     /gene="mdtC"
                     /locus_tag="ACHURQ_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR0823163.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHURQ_001338"
     CDS             complement(6697..9819)
                     /gene="mdtB"
                     /locus_tag="ACHURQ_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR0823164.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHURQ_001339"
     CDS             complement(9819..11060)
                     /gene="mdtA"
                     /locus_tag="ACHURQ_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR0823165.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            11339..11395
                     /locus_tag="ACHURQ_001340"
     CDS             11339..11395
                     /locus_tag="ACHURQ_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0823166.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            11666..11722
                     /locus_tag="ACHURQ_001341"
     CDS             11666..11722
                     /locus_tag="ACHURQ_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0823167.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHURQ_001342"
     CDS             11995..12054
                     /gene="ibsA"
                     /locus_tag="ACHURQ_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR0823168.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            12275..12934
                     /locus_tag="ACHURQ_001343"
     CDS             12275..12934
                     /locus_tag="ACHURQ_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0823169.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            12931..13692
                     /gene="pphC"
                     /locus_tag="ACHURQ_001344"
     CDS             12931..13692
                     /gene="pphC"
                     /locus_tag="ACHURQ_001344"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR0823170.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIHQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            13689..15629
                     /gene="yegI"
                     /locus_tag="ACHURQ_001345"
     CDS             13689..15629
                     /gene="yegI"
                     /locus_tag="ACHURQ_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR0823171.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(15642..16994)
                     /gene="yegD"
                     /locus_tag="ACHURQ_001346"
     CDS             complement(15642..16994)
                     /gene="yegD"
                     /locus_tag="ACHURQ_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0823172.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            17128..17976
                     /gene="alkA"
                     /locus_tag="ACHURQ_001347"
     CDS             17128..17976
                     /gene="alkA"
                     /locus_tag="ACHURQ_001347"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR0823173.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(18086..21403)
                     /locus_tag="ACHURQ_001348"
     CDS             complement(18086..21403)
                     /locus_tag="ACHURQ_001348"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0823174.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            21721..22362
                     /gene="udk"
                     /locus_tag="ACHURQ_001349"
     CDS             21721..22362
                     /gene="udk"
                     /locus_tag="ACHURQ_001349"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR0823175.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            22454..23035
                     /gene="dcd"
                     /locus_tag="ACHURQ_001350"
     CDS             22454..23035
                     /gene="dcd"
                     /locus_tag="ACHURQ_001350"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR0823176.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            23057..24910
                     /gene="asmA"
                     /locus_tag="ACHURQ_001351"
     CDS             23057..24910
                     /gene="asmA"
                     /locus_tag="ACHURQ_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR0823177.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(25184..26767)
                     /locus_tag="ACHURQ_001352"
     CDS             complement(25184..26767)
                     /locus_tag="ACHURQ_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0823178.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
BASE COUNT         6388 a         7194 c         7232 g         6095 t
ORIGIN      
        1 tcagtttgct gaccatgcgt gggataatgt tgccggatct ggtctgaacg ccatctccgg
       61 ctaacaaagt catgtcgcta aaactaaacg atgcggcagg cgtcggcttc ccagcggtaa
      121 ccaacgccgt aaacggcgcg gataaatgac tgttcggcgt cgagagattc cagcttgcgg
      181 cgcaggtttt taatgtggct gtcaatggtg cggtcggtta ctacgcggta gtcgtcataa
      241 agatgattga gcaattgctc gcgggagaac acttttcctg gttcatgaga gagcgttttc
      301 aacagacgaa attccgcagg cgtcaggtca agcattttac cgcgccatga agcctgaaaa
      361 cgaccttcgt cgataatcaa cgggctttcg gcatcctgtt gctgcaattc gcgctgcggt
      421 ttgcaacggc gcaaaatggt tttgacgcgc gctaccactt cccgtgggct gtacggcttg
      481 cagatgtagt catctgcgcc aatctccagt ccgagcaggc ggtcgatctc ttcgattttt
      541 gccgtcacca tcacgatagg aatgtcagaa aaacgacgaa tttcccggca cagcgtcaag
      601 ccatcggtgc cagggagcat cagatccaac aggatcagat ccggtggtgt ctggcgcaca
      661 tacggcagta cctgatcgcc gtggctgata agcgtcggcg catagctcgc agcacgcaga
      721 taatcaatga gcaactgccc cagcttcggt tcatcttcca cgatcaaaat acgcggtgtg
      781 ttttcgtcga ttggtaactc ggtcatactt ctctctgtaa atcccgttcc agcggtaact
      841 ctactgtaat gcttaccccg ccaaaaggcg aatgggcagc aataatgcgg ccattatgtg
      901 cttcaacaat gttcaggcaa atcgccagcc ccagcccgga accaccgctg gcgcggttgc
      961 gggaaccttc ggtgcgataa aaacgttcaa ataatttttg tagctgctcg tcactgacac
     1021 ctggcgcact gtcggcaaag gtcaggcgca ccgttttgtc gtgctgctcg gcagagattt
     1081 tcaggctgcc gccgctgtca gtgtagcgca ggctgttttc aagtaaatta ttgaataact
     1141 gcattaaacg gtcgcgatcg ccaaatacgg taatactgtc tggcagggaa aattgcagtt
     1201 tcaggccacg gctggcgaat cgttcgcgaa atgcgccgcc cgccacttcc agcagtggga
     1261 tcaaatctac cggtgctttt tgataggcga gggcgccttc atcagacatc gacaactgat
     1321 ggagatcgtc aaccagtttg gtcagtgtac cgacctccgc ctgtaaagac gccaccgtct
     1381 ccggcgtgaa tttgcgcacg ccatcctgaa tagcttccag ttcaccgcgc agcaccgcta
     1441 atggcgtacg cagttcgtga gaaatatcgg ccataaaatc gcgccgcatt tgctggtttt
     1501 tctccagtgt gctggcaagc tggttgaagt cttgcgccag tttgcccagt tcatcttcac
     1561 tggtgggcgt tacgcgggta gtgaaatcgc ccgccgccag tttgtgcgtg ccatcgacaa
     1621 gtcgttttac cggtgccagt aaaccgcgcg ccagcagaaa agtggcaagt gccgcgagta
     1681 acgttgccag ggcgacaatc aaccagctgg tttgccgctg ttgtttatcg aaattgatat
     1741 cagtattgcg cgttaaccgc tcaacggggg aggcgatcac cgcgccaact tccgcaccgt
     1801 tgaccagaat gggtcgccgt gtaccgtcag gtggaatcgg cgctcgcgga ccaaccagca
     1861 ctttgttgtt ttgatcaacc acccagaact gggtacgcca gccgtgcggt ggcatacccg
     1921 ggccgggttt atcttccgaa ttatcgtgtt caaatgaacg caggatctga aagacaaagc
     1981 gatcattgtt gcgcaggaag cgccagttgc catgctgcgc atactgctcg ccaagcgcat
     2041 cacttaacag ttgtaatcgc tgttcattac catgcttgat gtaatcaata aagccacgct
     2101 caaaactgat acgcaccgcc cagtgcatac tgatcagcaa aacaatgcag gtggcgaaaa
     2161 tcgccagaaa cagtttgccg gtaataccag gtcgccagaa cttcattgcg cgctcctttt
     2221 tcgccgcgaa atagctacat tttgatgcgt atcgttcggc actctggcaa agatgaacgc
     2281 cggaagggcg atgataaacg ccatgctaag ccaggtgtac ataaagacgg tttgtgtggt
     2341 gccgctgtcg acgctgacat gctgcgaacc aaaaagtccc agcaacagcc cggcgatggt
     2401 gacgccgata ctcatcgaca attgcataat catcgacagc agactgttgc cgctgctcgc
     2461 cagattgtcc gggagatctt tcagcgtcag ggtgttcatg gaggagaaac gggtcgagtt
     2521 gaccatccct tgtaaaaaca ggacgaacgg caaaacgtag taccagccca acagagcggt
     2581 agtcataaac aacagggtga tcagcgacag acccagcgtg gtcgctacca gtacccgacg
     2641 ataaccaaag cgattcacca cctgaaccac aattcgcttc attcccatgc tgccaagtac
     2701 catcgggatc atcatcagtc cggcatgaaa cggcgagaaa ccgaggccaa tttgcaggaa
     2761 aaccggtgtc ataaagggca acatgccact gccaatacgt ccggcaaagc tccccgccag
     2821 gcccagcgaa aaggtacgag tgcggaacag tttcagactg aacagggcgc ggttgttatt
     2881 tctggcgtgc agcagataaa gcaccagtgc caccacgcca actgcgacca ggccagcaat
     2941 cgccagcggc gataaacctg tacctttact gccgtccagc gccagggtta atacagccat
     3001 gccaaccgcc agcaataaaa agccggaaag atcaaagcgc cgcgtctgca tggtgtagtt
     3061 cggcattaac agcaatgtgg cgatcgcacc gataatcccc accggaatgt tgatcaaaaa
     3121 gatccagtgc cacgacgcgt actccaccag cagaccgccg agcgccggac cgagcagcgg
     3181 accgacctga ccgggtaacg tgacaaaggt catcgccgcc atatattgct cgcgcggtac
     3241 gattttcatc accgtcaacc tgccgaccgg caccatcatc gcaccgccaa cgccctgtaa
     3301 cgcgcgtgcc agcaacagtt cgttcagcgt gccggaaagc gcgcaaaaca gtgaaccgag
     3361 agtaaacagc acgatggcgg taaagaaaat attgcgcacg ccgactttgt ccgccagcca
     3421 gccgctggcg ggcagcatca ccgccacggt cagcacataa gagacaatga ccatgtgcat
     3481 atgcaacgga ctttccccga ggctttgcgc cattgaggga agggcggtgt taacgatggt
     3541 ggtgtccagc gactgcataa agaagccgaa agccacaatc cacaattgcc agcgggtgct
     3601 gtcgggaagt tctgtcattt actcggttac cgtttgttta ggtttacgcg aaaaacgcag
     3661 ccgcagacgg tcgaaaaaga gatacaccac cggcgtggta tacagcgtca gaagctggct
     3721 cattaccagt ccaccgacaa tggtgatccc cagggggtgc cgcagctccg agccgtcacc
     3781 gcccgacaac accagcggca gcgcgccaaa cagcgccgcc agggtagtca tcataatcgg
     3841 gcgaaaacgc agcagacagg cctggaaaat agcttcctgc ggcgtcaggt taccgtgccg
     3901 ttgggcttcg agcgcaaaat cgaccatcat aatggcgttt ttctttacga tgccgattaa
     3961 tagcatgatc cctatcaggg ctattaggct gaacggggca ttgaacagct ccagcgccag
     4021 cagcgcacca acgcccgccg agggcagggt ggagagaatc gtcagtgggt gtacgtaact
     4081 ctcgtaaagg atacccagca cgatatacac cgtggcgatg gcggcgataa tcaggatcac
     4141 ctgtgagttc atcgtctcct ggaacacctg cgctgttccg gcaaaactgc cgcgcaccgt
     4201 cgaaggcaca ccaagctggg tcattgcgcg atcgatcgcc gcactggcgt ccgagagcga
     4261 ttttccggtc ggcaggttga acgagatggt cgaggccgcc gataatccct gatgattcac
     4321 cgacagcgga gcgttcgccg gttgccattt agcgaaatac gacagcggga tcgctttgcc
     4381 ttcgttattg ataacgaaca ttttttccag cgcactgatg tcctgggtat agcgcggatc
     4441 cacttccatc accactttat actggttcat cggctggtaa atggtcgaga tttgccgctg
     4501 accgaaggcg ttatttaaca ggctgttggc ggcctgcacg tcgattccca gccgtgccat
     4561 ggtgtcgcgg tcgtaaacca gattcatctc cgcgccgtta tcctgctgat cggagttcac
     4621 gtccgccagt tccggcaacg tcgccagttt tttgcggatt ttcggctccc attcgcgcag
     4681 tgccgccagg tcgtcggata acaacgtgta ctggtagctg gcgttcgact gacgcccacc
     4741 aacgcgaata tcctgtaccg ccatcaggaa cagattcgcc cccggttctt tcgccagttt
     4801 tacgcgcaga cggtcgataa tttgctgcgc cgtttcgctg cgttcgtcgc gtggcttgag
     4861 ggtgataaac atcatcccgc tgttcactcg cgaaccgcct gtaaagccgg tgacattatc
     4921 cactgccgga tcgtcacgga taattttcat gaaatcctgc aacttaccgc gcatcgcctg
     4981 aaacgaaata ctctgatccg cctgaatccc gcccatcaac acgccagtgt cctgctccgg
     5041 gaagaaggtt ttcgggatcg agatatacag ccagatattc agcgcaatgg tgccaagcag
     5101 caccacgccc accagacggg tatgattgag cacccatttc agtgacttgc cgtagccttg
     5161 ttgcagggct accaacatgc gaccaaaacc acgcagtcgc ttttgctcgc gcggcttgct
     5221 ggctttcagc atccagccac acatcattgg cgttaatgtc agagaaacca gcaacgaaat
     5281 accaatggcg acagaaagcg tcacggcaaa ctcgcgtaac agtcggcccg gcaatccgcc
     5341 catcaacagc agcggcagga acaccgccac cagtgacaga ctcatcgaca gcaccgtaaa
     5401 accgacttcg cgagtacctt gcagtgcggc ttgcaacggt ttcattcccg cttccagatg
     5461 acgtgcaatg ttttccagca ccacgatggc gtcatccacc acgaaaccag tagcgatggt
     5521 gagcgccatt aacgaaaggt tattgagact gaatccgcac aggtacatcg ccgcaaacgt
     5581 accaatcagc gaaaccggca ccgaaacggc gggaataata gtggcgcgac ccgagcgcag
     5641 gaataaaaac accaccagaa tcaccagcgc caccgagata atcagcgttt gctcgacttc
     5701 ttccagcgag gcgcgaatgg tgggggagcg atcctgggca atttgcagat caatcgccgc
     5761 cggaatggtt tcctgcaact ccggtaattt tgcccggatg ctgtcaaccg tctggataat
     5821 attggcttcc ggcagtttgc ggatcatcag taaaatagcc ggtttggcgt tggtcatccc
     5881 ggcgttgcgc acatcctgca ctgagtcggt caccgtcgcc acatcgccca gacgaaccgc
     5941 gccgccgttg ttgtagtgaa taatcaacgg ctgatattca gcggcggttt ttagctcatc
     6001 attggtctgg atctgccagc ggtgagtgcc atcttccagc gcgccctgcg gtttacgcac
     6061 gttggcattg ctgatggcgg tgcgtacgtc gtccagcgac acgccctgat taaacagcgc
     6121 ctgcggattc agcccgacgc gtacggcggg cagtgagctg cctccgacat cgacatcacc
     6181 aacaccgtcg atttgcgaaa tcgtcggagc cagctgcgtc gaggcgaaat cgtacagttc
     6241 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
     6301 cgctttgcga taggtcggac ggctgggcat cccactgggc agcaaacttt gtgcagcgtt
     6361 gatcgccgcc tgcacatcac gcgctgcgcc gttgatgtcc cggtcaaaat caaactgcaa
     6421 aataatacgt gtgctgccga gcgaactgct ggaggtcatt tcgctgaccc cggcaatgcg
     6481 cccaagcgag cgctccagcg gcgtggcaac ggaagacgcc attgtttctg gtgacgcacc
     6541 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
     6601 cagcatacgg aagcccagta tgccgcacag ggtaatggcg accgacagta aaatcgtcgc
     6661 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catggcgagc
     6721 aaagcgactt ttggtccaca atgccaggcg gtcgaacagc agataaatca ccggcgtggt
     6781 gaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
     6841 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
     6901 cagggtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcatcgcg
     6961 cggcgacatg ccttgctcgc gctcagcagc cagcgcgaag tcgatcatca tgatggcgtt
     7021 cttcttcaca ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
     7081 accggcaatc attaatgcca gcagcgcgcc gacccctgcg gtgggtagcg tcgagagaat
     7141 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
     7201 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
     7261 ctggaattgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatctc
     7321 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg tcgttaccgg
     7381 gaactgatcc agatggttga tggaaagcgg cgcaaaacgc tgttcaactt tggcaattga
     7441 gcttaacggc accacgccgc cgtcgctact ggtcaggcga atggtatcca gcgccgcgag
     7501 gcccggggta atttcggtgt tgtgctccag cactacgcga tactggttag cctgagtata
     7561 aatagtggaa atcagtcgct gaccaaacgc gttgtacagg gcgttatcga catccgccat
     7621 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttg
     7681 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
     7741 ctgtggcacc caggtactga gcgcatccag tgaggtggcc tgcaaggtaa actggtactg
     7801 ggtgcggctg acctgagtat caatagtcag atcctgcgtt ggttgcagga agagatcgac
     7861 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
     7921 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctattcagcg acgggttagt
     7981 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
     8041 cgcgacctgg cgttgtcgct gggccatatt ggtaaaggag ctggattgcg gtgcctgcaa
     8101 agtgccctga ataatgccat tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
     8161 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
     8221 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
     8281 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
     8341 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
     8401 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
     8461 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
     8521 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
     8581 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
     8641 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
     8701 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
     8761 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
     8821 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
     8881 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
     8941 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
     9001 ttgctctaca gttgcgacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
     9061 tagctggcga tactcttcgg cggattgcat ctggtcgttc gcggaaagcg tgactgcacg
     9121 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
     9181 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
     9241 agccggacgc tgaccgccgg aaagcgtcac caggccgacg ccggaaatct gcgagatttt
     9301 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
     9361 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
     9421 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
     9481 ggcgacatcg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
     9541 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt cgagcggcgc
     9601 ggtaacggca gaggtcatga catccgggct ggcacctggg tagagcgtga ccacctgaat
     9661 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
     9721 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaacaggcg
     9781 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
     9841 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
     9901 tccactttcg ccccttcggt caggcgatca atgccgtctg tcaccacgcg atcgcccgca
     9961 gaaatacctg cacggatcac cactttctga ctgtcctgaa tgcccggcgt caccagatgt
    10021 ttgctgacct tgttttcgct attcagcacc cagacaaaat ggccttcatt gcccatttgc
    10081 agggcggctg ttgggatcac tacggcgttt tgttcggtgt cgactaacat gcgcgcgtta
    10141 acaaactgat tgggaaacag cgcatcatcc tgattattaa agcgtgcttt cactttaatc
    10201 gtaccggttg tggcatcgat ttggttatcg aggcttaaca gcgtgccttc acttaatttc
    10261 ttcgaattgg tgcgatccca ggcttctacc accagcggtt ttccggcttt ttgcgcctgc
    10321 actacagtag cgatatcgct ttccggcagg gtaaagacta aatcgatagg atgcgtctgg
    10381 gtgatcacca caattccggt ggtatcacca ctggagattt ggttaccaac atcaacctgc
    10441 ttgagaccaa cgcgaccatc gactggtgcg gtgatgcggc tccagtcgag ttgcaactgc
    10501 gcgctggcga cgcttgcttc atcagcctta atggtgcctt cggtttcact gaccagcgcc
    10561 tgttgggcat ccagctcctg acgggagacg agattggttt ttgccagttg ctgataacgc
    10621 gccagatcac ggcgggcgtt agcaagcgtg gctttatctt ttgccagttg gccctgggtt
    10681 tgtgctaatg caactttgaa ctggctgggg tcaatttctg ccagtaaatc gcctgctttg
    10741 acctgctggc cttcctggaa atgtaacgcc atcagttgac cgtccacgcg gctgcgcacc
    10801 gtaacagtat tagcggcgat aatggtgcca agcccggtga ggtaacgcgg aactgcctgt
    10861 tctacggcgg tcgccgcctg aaccggggct aatgggccgg aacgcatacc acggcgacca
    10921 cccgctggcg attgctgcgc ttgtttcgtc gcccctgggg ctgcactctg ggagtcattg
    10981 cggccttgcc agaaccagaa tgcggcgatg gcggcgataa ccaccacgat tacccaacgg
    11041 gatttataac tgcctttcat cgttaagagt ttctcttcct gaaacgtttc gcggaatgat
    11101 actagtgtag ccagccaata agggagaaaa atggaggaat tatgaaacac tgtcgggaat
    11161 cgtctgaatg acgggcacat ttgcgagcac gcatccagta ataacacagg aaactatttt
    11221 atctacgcgt tagcgataga ctgcttgcat ggcgaaagga ggtaagccgc cgatttcagc
    11281 gggacgctga aacgggaaag cccctcccga ggaaggggcc ataaataagg aaagggtcat
    11341 gatgaagcta ctcatcatcg tggtgctctt agtcattagc tggccagctt actaaggcta
    11401 ccagggcggg ggaaaccccg ctctaccctc actcctgaaa gtatgccttc acgataagat
    11461 tgtcaatctg caggctttgc agtctgcgat cctgccagca aatattcttt gcgagacgtt
    11521 acgcaataat cacactggaa actattttat ccgcaagtca gcgatagact gcatacaggg
    11581 cgaaaggagg taagccgacg atttcagcgg gacgctgaaa cgggaaagcc cctcccgagg
    11641 aaggggcctt aataaggaaa gggtcatgat gaagctactc atcatcgtgg tgctcttagt
    11701 cataagcttc cccgcttact aagactacca gggcggggga aaccccgctc taccctcact
    11761 cctgaaatta tgccttcacg ataagattgt caatccgcag gctttgtagt ctgcgatcct
    11821 gccagcaaat attctttgcg agtcgttacg caataatcac agatgaaact attttgttca
    11881 cgcgttagcg atagactgca ttcagggcga aaggaggtaa gccgacgatt tcagcgggac
    11941 gctgaaacgg gaaaccctct cccggagaag agggctttta ataaggaaag ggttatgatg
    12001 aagcacgtca tcatactggt gatactctta gtgattagct tccaggctta ctaagaacac
    12061 cagggggagg gggaaacctc tccctaaccc tcactcctga aattatgtca ctccgccctt
    12121 agtgtcaatc gacggctctc cgttcttgcg aggcgaatag caaaattttc tgcgccgacg
    12181 ggcctaattc tgaaagtttc ttcgtttgct cttatttcat tattgcaatc ggtatgatac
    12241 cggacctcga taattctcta agaaaggatt tcttatgagc gaacaaatca cgtttgccac
    12301 cagcgatttt gccagtaacc cggaaccacg ttgcccctgc attttgctgc tggatgtttc
    12361 tggctcgatg agcggcagac ctattaatga acttaacgcc ggattggtta cctttcgtga
    12421 tgaactgctt gctgatccgc tggctttaaa aagagtggaa ctcggcattg tgacgttcgg
    12481 cccggtgcat gtggaacaac cctttaccag cgcagctaac tttttcccgc ccatcctgtt
    12541 tgcccagggg gatacgccta tgggggccgc cattaccaaa gccctggata tggttgaaga
    12601 gaggaaacgt gagtaccgcg caaatggtat ttcgtattat cgcccgtgga ttttcctgat
    12661 caccgatggc gctccaaccg atgagtggca ggctgccgcc aacaaagtgt ttcaggggga
    12721 agaagataag aagtttgcct tcttttccat cggtgttcag ggggcggata tgaagactct
    12781 ggcacaaatc agcgttcgtc agcctttgcc actgcaggga ttacaattcc gtgaactgtt
    12841 tagctggtta tccagttcgc tgcgttcggt ttcccgctcc acgccgggaa cggaagttgt
    12901 gctggaagcg ccaaaaggct ggacgtcagt gtgagctggc gtctggttta tgcatccgct
    12961 gttggaacat cgcacattag tgctgatttg ccttgtcagg atgcctgcca gatgcaggtt
    13021 gtctggttaa acgaccagca accattactg gtgatgttcc ttgctgatgg cgcaggtagc
    13081 gtttcgcagg gaggagaagg cgcgacgctc gccgtcaatg aagcgatggc gtttatggtg
    13141 caaaaagtac aggacggtga attggggctt aatgatgtcc tcgccacaaa tatggtactg
    13201 actattcatc aacgactttt tgccgaggca gaagccaaag cattagcagt tcgcgatttt
    13261 gcctgcacgt ttctgggatt gatttcatcg cctgacggca ccttgattat gcagattggc
    13321 gatggcggcg tggtggtaga tttgggtcac ggtctgcaat tgcctctcac gccgatggtg
    13381 ggtgaatatg ccaacatgac gcacttcatt accgacgaag atgccgtttc cagactggaa
    13441 actttcacca gcactgggcg tgcgcataaa gttgcagcat ttacggatgg tattcagcgg
    13501 ctggcgttaa atatgctgga taattctcct catgtgcctt tctttacccc gtttttcaat
    13561 ggactggcgt cagcaacgca ggaacaactc gatttattac ctgaattgtt aaagcagttt
    13621 ttgtccagcc cggcggtaaa cgagcgtacg gatgatgata aaacgttggc actggcgctg
    13681 tggttaccat gaaacccact ttatatactg ctactggtga gtgcgttacg ccaggccgtg
    13741 aactgggcaa aggcggtgaa ggcgcggttt atgatatcaa tgagtttgtc gatagcgtcg
    13801 ccaagattta tcacacgccg ccacccgcct taaaacagga caaacttgcc tttatggctg
    13861 cgacagctga cgcgcaattg ttgaattatg tcgcctggcc gcaggcaacg cttcacggtg
    13921 gacgaggcgg aaaagttatc ggttttatga tgccaaaagt ttctggtaaa gaaccgattc
    13981 atatgatcta tagcccggca catcgtcgtc aacgttaccc tcattgtgcg tgggattttc
    14041 tactctatgt tgcgcgcaat attgcttcat cttttgctac ggttcacgag cacgggcacg
    14101 tcgtgggtga cgtaaaccag aacagtttta tggtaggtcg cgacagcaaa gtggtgttga
    14161 ttgatagcga ctcctttcag attaacgcca atggcacact gcatttatgc gaagtcggcg
    14221 tgtcgcattt tacgccgcca gagctacaaa ccttgtcgtc atttgtcggc tttgaacgca
    14281 cagaaaatca cgataatttt ggccttgcgt tgctgatttt tcacgtcttg tttggtggcc
    14341 ggcatcctta ttctggtgtg ccgcttatct ctgatgcggg taatgcgctg gagacggata
    14401 ttacccattt ccgttatgcc tacgcgtcag ataatcagcg acgtggttta aaaccgccgc
    14461 cacgatcgat tccgctatcg atgttaccga gtgatgttga agccatgttt cagcaggcgt
    14521 ttacggaaag tggcgtggca accgggcgtc cgacggctaa agcgtgggta tcagcactgg
    14581 attctctacg ccaacagtta aagaaatgta ccgtttcggc aatgcatgtt tatcccggtc
    14641 atttggctga ctgcccatgg tgtgcgctgg ataatcaagg tgttatctat tttattgatc
    14701 tcggcgaaga ggtcattacc accggcgggg attttgtgct ggcaaaagtc tgggcgatgg
    14761 tgatggcgtc agtagcaccg ccagcattgc aactgccatt acccgatcat ttccaaccga
    14821 ctggcaggcc gcttccttta ggcctgttac ggcgcgaata catcattctg cttgagatcg
    14881 cactgtcagc attatcgctg ctgctttgcg gccttcaggc agaaccgcgt tatattattt
    14941 tggttcctgt gctggcggct atctggatta ttggcagttt gacaagcaaa gcgtacaaag
    15001 cagaagttca gcaacgccgt gaggcattta atcgtgcgaa aatggactat gaccatttag
    15061 tccgccagat ccagcaggta ggcggcctgg aaggttttat cgccaaacgg acgatgctcg
    15121 aaaaaatgaa ggacgaaatt ctcgggttac cggaagaaga gaaacgcgct ctggcagcac
    15181 ttcatgacac tgcaagggaa cggcagaagc agaagtttct ggagggattt tttattgatg
    15241 ttgcctctat tcccggcgtt ggccctgcgc gtaaagcggc gttacggtct tttggtattg
    15301 aaacagccgc agatgttacc cgtcgtggcg ttaagcaagt gaaagggttt ggcgaccatc
    15361 tgacccaggc ggttatcgac tggaaagcga actgtgaacg ccgttttgtg ttcaggccga
    15421 atgaagcgat aacgccagca gacagacaag cggtaatggc gaaaatgacc gccaaacgac
    15481 atcggctgga atcggcgttg acagtcggcg cgacagagtt gcagcgattc cgccttcatg
    15541 ctccagcacg gaccatgccg ttgatggaac cgttacgtca ggcagcagaa aaactggctc
    15601 aggcgcaggc agatttaagc cgctgctgag ccatttttca attaacgaaa caccacttcc
    15661 gcccagcgtg ccagcccggc ggtgacggag ccaaagtcat cgccgcctgc aatcggaatg
    15721 cccggcaact gttctgccag cgctttttta atctgcggag aacgggcgct accgccggtc
    15781 aggtagataa cgtccggttt ttcctgggcg ttatccaggg ccagttgcac ctgttccaga
    15841 atccgcgcca gtggctggct gagggcgctt tccagccctc gctggctaat cagcgtagcc
    15901 agttcattgc tgataaacgg cagtgaggcg cgggtttccg ctacgcttga aagagcaatt
    15961 ttgcactctt ctgcgctacg caccaggcga tagcttaaac gctgacgcca gactttctgt
    16021 aacagggcca ctttttccgg ttcgcgggca tcgcgtacca gatcgttaag cagacgaccg
    16081 ttggcactac tgtagaaatc actctgcgca ggtacgtcgt tgatggcaac cgcattccac
    16141 cacggcagga tcggcagggc gatgcctttt tcggtttcgc cacccatacc cagcagtggc
    16201 atcaggtttt taaacgccag ggcgatatcc agatcgttac cgccaatacg acaaccactg
    16261 tgacccagca gactggcttc acgatcgaga cgcgaacgcc actgcggccc catcagcagc
    16321 aatgaacagt cagtcgtacc accgccgata tccaccacca gcacccgttt ttcttcctgc
    16381 aaggtggctt cgtaatccag cccagccgcg atcggctcgt actggaatac cacgtccctg
    16441 aatccggcac gcttcgccgc gcgttccaga atctcttgcg cctgggcgtt tgcttcatcg
    16501 ccgcccagcc cctggaagtt gatcgggcga ccaatcaccg cctgagtaat cgcttctggc
    16561 agctgcgcct gcgcctgctg gcgaatgtgc aacatcattg cgcagaccag atcctcaaac
    16621 agcgctacct gctgcggttt taagccgctg gcaccgagga acgattttgg tgatttcaca
    16681 aaccacactt cttctggatc atcaatgtac tgtgccagtg aggaaagacc gaactgcacg
    16741 cttttcgccg taacatcgat atcttcttcg cggttataac gaatcgcccg acgtaacagc
    16801 gcctgcgttt catcgtcgtc tgccggaaca tcatgatggc ggtacagcca ttcgcttacc
    16861 gattcacgcg ttggcgcgca aagcattgaa ggcagcagcg tgctgtcgtt ttccattttc
    16921 agtaattgcg gtttaccgtc acgcatgacc gccactgaac agtttgctgt accgtaatca
    16981 aaaccaataa acacgaatta atccccatgc cggtgaagaa ggggcgtgac tttagcgaaa
    17041 tgttgctgtc gcgacaaccg gaatatgaaa gcaaagcgta acgtctgaat aacgtttatg
    17101 ctgaaagcgg attaacaagg agatgcgatg tataccctga actggcagcc gccgtatgac
    17161 tggtcgtgga tgttgggatt tctcgccgcc cgtgcggtga gcggtgtgga aacggtcgcg
    17221 gacaattatt atgcccgtag tctggcggtg ggcgaatatc gcggcgtagt gactgctatt
    17281 ccggatatag cccgccatac tctgcacata aatttaagtg caggtttaga acctgttgcc
    17341 gcagagtgtc tggcgaaaat gagccgcctg cttgatctgc aatgtaaccc gcagattgtt
    17401 aacggtgcgt tgggaaagtt aggcgcgggg cggcccggat tgcgtttacc cggctgtatt
    17461 gatgcttttg agcagggtgt gcgggcgatt ttaggccaac tggtgagcgt ggcgatggcg
    17521 gcaaaattga ctgccaaagt ggtacagctt tatggcgaac ggctggatga ttttccggag
    17581 tatatctgct tccccacgcc tcagcggctg gcagcagccg acccgcaggc attaaaagcg
    17641 ttaggcatgc cgttgaaacg ggcagaggca ctgattcatc tggcaaatgc ggcgctggag
    17701 ggcagcttac caatgacaat tccgggcgat gtggagcagg cgatgaaaac gctgcaaact
    17761 tttccgggta tcgggcgctg gacggcgaat tattttgctt tgcgtggctg gcaggcgaaa
    17821 gatgtttttc tgccggatga ttatctgatt aaacggcgat ttccgggaat gacaccggca
    17881 caaatccgcc gttatgccga gcgctggaag ccctggcgtt cttatgcgct gttgcatatc
    17941 tggtatacgg aaggctggca accagacgaa gcatgacgtt ggtcgccgga tgcggtgcga
    18001 gcgccttatc cggtctgggg aatgagcgat ttgtaggcat gataagacgc gttagcgtcg
    18061 catcaggcaa tgggcaaacg ctctctcagt taatcgcgaa ataactgcta ttcaccagca
    18121 aatccagcgg ttgggcatcg gcaatcacat caccataaat cagatcgacg ccgatgccag
    18181 aaagcgtatc catcactaag ggtaaaacga ctggcccggc gatggttttc atcccgagtc
    18241 gctgagcgtg cccctgaata atcgtaatca gcatctcatc catcaaatta ccctgcacgt
    18301 tggcgcataa ctcaccatca agtagcaggt aatctgccat attcgctttc agcgagttga
    18361 agatttgcag atcgcggccc acctggctga gcactatccg acatcccgcc agctgcagtt
    18421 tttgcacgct ttctgcgtga tctaaaatcg cttcagccgg aataatcaga tgtaataacc
    18481 gtggtggtag agggctattt tccagctgct caagcagatc attcaccagc gtggcgctac
    18541 tcaaaccggc aacggaaagg gggagggcga tgcttatgcc tttactggca accgctttgg
    18601 cggcctgctg gaaaaattcg tggaataccc gtcggtcaag agcatggcta agtgccggat
    18661 cgctgaagct acgacgaaat gtttgttcat caataatttc gccttcgcaa ctccagagct
    18721 taagcgaaat cagccacaaa ttacgcgctt ccgggatccg cggcgaagcg acaccgtggg
    18781 cgatcatcat caactgattc tctttaatca tccgccactg ttcatcaagc gacatcgccg
    18841 cccgttcgct atgtgcggca gcttgctgtg gttcgtaaac cgtcacccgg ccccgtccac
    18901 catttttgga ggcataacag gcgatatcag cctgcgacat cacttctgcc gcctgatgat
    18961 tgttgtcatc gatcaaggta atcccggcgc tggcacctac ccgatgtacc cggccttccc
    19021 atataaagtg atagtcattc acagcactga taatacgtgt agcgataaaa cgagcgcttt
    19081 caacattgca gtctggcagc agcagaccaa attcatcacc gccgagccgc gccagcacgt
    19141 cactggagcg cagcatactc agcatcaacg acgccagttc gcgcagcaaa gcgtcgcccg
    19201 ccgcatgccc ggcgctgtca ttcaccgctt taaagcgatc aagatcgata aacaccaggg
    19261 catgtcgctg atgcgtactg tttaccgttt gcagcaggat acgcagttgt ttctcaaaac
    19321 tggcgcggtt ggcgagatgc gtcagtgcat catgggaggc gctgtagctc agctggcgca
    19381 gcattttgcg tgattcggtg acgtcctgaa tcaccagaac cgaaccaata ttgctgccgt
    19441 ccagagtact taacggcgta atactgtaat gcacgtcgta actgccgccg ctccgacagt
    19501 gcaacaccac atcttgttcg attgcggaac gtgaggtgtc ggcactgtaa atgttctcca
    19561 ttaatggtcc gttgtcgcca aaagtaatat gcaacaccgt caggagcgga acacctaacg
    19621 cttcttcctg cgtccagccg ctcatcttct ccgccactgg attcataaag gtaattttca
    19681 tcgccatatc aatacagacc acggcttcac cgatggaatc aagcgtaatg tgcaggcgct
    19741 ctttttcctg aaacaatgcc tcgttaagct gtttcacttc ggtcatatcc atattgatgc
    19801 cgaggagacg ttcgacttcg ccttctttat tcagtacccg gttggcgagg gcgcggatat
    19861 ggcgaatacc gtcttttacg gtaatgcgaa attccagttt aaagggcgag cgtgattgca
    19921 acgaatcacg aatcactttt tcggcatgct ggcgatcttc cggcagcacg cactcgtacc
    19981 acacctgcca gttcggtttg atatgtggag gaatttcata cagctcgaac atccgcttat
    20041 cccagctaaa aacattcggc ttcaactccc actcccagat gccaatcccg cccgcttcgt
    20101 tagccagcgt gatgcgctcc atcagttgct gattgacctg ttcggtgcgt tttagctcgt
    20161 taatgtcttc aatctgagcg ataaaataga gcggcgtgcc atccgtgtgg cgcaccagtg
    20221 agacggcaag caacgcccag acaacatcgc cattgcggtt gtagtagcgt ttttccattg
    20281 aataggtgtt tatttcaccg cttatcagct tttcaacctg ttggagatct ttattgagat
    20341 cctccggcca ggtcagttgc tgaaaggtga gtccgcgcag ctcttcctga ctgtacccga
    20401 gaaactggca gagcgctttg ttggtttgca gccattgtcc ctcggtgccc actaacgcca
    20461 taccgatagc ggaatattcc atcgcgttcc gaaagcgggt ttcgctttcg gaaatgtgtt
    20521 tgcgttccgc acgaaaggca tacatcacca tggtcatgat gttggcgggc agcaggatca
    20581 gcaaaaacgg tagccacggc atatggctca tcaggtacgt acgcggcgta gcaagcaggg
    20641 agggatccgc ggccatcatc agcgacacca tcatcaccgt ggtaaggaag atcaaaaagg
    20701 cttccattcg tggcaggcgc acggcgctcc acatcaacag cacaataatg aaagtaaaag
    20761 gccacggcag atacagcatc gaaagccagc ttaacgtcag tgtgatggct aacgtgagca
    20821 gcgactcaaa aagcaaccgt gggttgcgat ggcgcagcag atagtgtggt ttaaataaca
    20881 atcccagcgg caccagtgcc agagcgccga tggattctga cagtacccat atcaaaaatg
    20941 ccctgagagg atcgtctccg ggcgtcagca ggacaaccag aacacccccc aacagaggcg
    21001 gaacaatggc gctgccgagt gccagacgca gccagtcagc cagattttgc aaggggttat
    21061 accacggcag caatttacgt agcagcactg ccccgaccac ggcttcaaca atattgatgg
    21121 tcgtccaggt catgttcagc gagctggtgg aaaaaagcag gatggatgcg gcgatatttc
    21181 ccagcgaaca gctcagcgca attcccggcc acatgcgccc ggcatggcga taaaacgcca
    21241 ccatcatgat ggacgtcggg aaccacagtg gggcgagttg ggtgccaaac tgcgaaagct
    21301 caagcgagaa aagggtaaag ataaacgaga ctaaacctaa actgaccagg tgaagcagcg
    21361 ggtggggcag ggcaattaat acatgctgtg attgtttgct cattaccgct ttatccgcaa
    21421 gaccaggtcg ccagtattcg tttctgtgtt tatgctagta caaaagtttt acattttata
    21481 tgcgaattgc tcataaagtg acgtaaaggc ggataatttg cgcaactgcg tataacattt
    21541 tttaccttac ataaaactga tcaacgtaat ttgccaccgg gaattctttg tcaacaatta
    21601 actctgagcg cgcggcaacg ctattcgact ggtatcagac ggatgaaatc cctataattg
    21661 ccgcgtttgg cgcttcgtcg cccccttcct aacatccagg ttaatcaggt cgctaaatat
    21721 atgactgatc agtctcatca gtgcgtcatt atcggtatcg ctggcgcatc ggcttccggc
    21781 aagagtctta ttgccagtac cctttatcgt gaattacgtg agcaagtcgg tgatgaacac
    21841 atcggcgtaa ttcccgaaga ctgctattac aaagatcaaa gccatctgtc gatggaagaa
    21901 cgcgttaaga ccaactacga ccatcccagc gcgatggatc acagcctgct gcttgagcat
    21961 ttacaagcgt tgaaacgcgg ctcggcaatt gacctgccgg tttacagcta tgttgaacat
    22021 acgcgtatga aagaaacggt gacggttgag ccgaagaagg tcatcattct cgaaggcatt
    22081 ttgttgctga cggatgcgcg tttgcgtgac gaacttaact tctccatttt cgttgatacc
    22141 ccgctggata tctgcctgat gcgccgcatc aagcgtgacg ttaacgagcg tgggcgttca
    22201 atggattccg tgatggcgca atatcaaaaa accgtgcgcc cgatgttcct gcaattcatt
    22261 gagccttcta aacaatatgc ggacattatc gtgccgcgcg gcgggaaaaa ccgcatcgcg
    22321 atcgatatat tgaaagcgaa aataagtcag ttctttgaat aagcttgata aattgtgtac
    22381 cgttcagtga taacctggta tgcccttgac gtaattggcg ttaagggagt gatgcgcgaa
    22441 aggagaaaat gccatgcgtc tgtgtgaccg agatattgaa gcctggcttg atgaaggccg
    22501 tttgtcgatc aacccacgtc cgccagtgga gcgtattaac ggcgcgacgg tggatgtacg
    22561 cctgggcaat aaatttcgta ccttccgtgg tcacacggca gcgtttatcg atctgagcgg
    22621 tcccaaagat gaagtgagcg ccgcgcttga ccgcgtgatg agcgatgaga tcgttctcga
    22681 cgagggcgag gcgttctatc ttcacccagg agagctggcg ctggcggtga cgctggagtc
    22741 ggtgacgctg ccagccgatc tggtgggctg gctggacggg cgttcctctt tggcgcgtct
    22801 ggggctgatg gtgcacgtca ccgcgcaccg tatcgatccg ggctggtctg gttgcattgt
    22861 gctggagttc tacaactccg gtaagctgcc gctggcgctg cgtccgggca tgttaattgg
    22921 tgcgctgagc tttgagccgc tttccggccc ggcggcgcga ccttacaacc gccgtgaaga
    22981 tgcgaaatat cgcaaccagc agggcgcggt agccagccga atcgataaag actaattcag
    23041 gcccattgag gataccatga gacgatttct gacgacgctg atgatactcc tggtcgtgct
    23101 ggtggccggg ttatctgcgt tagtgttgct ggtgaatccg aatgatttcc gcgactatat
    23161 ggtcaagcaa gttgctgcac gtagcggtta tcaattgcag ctcgacgggc cactgcgttg
    23221 gcacgtctgg ccgcagctta gtatcctctc cgggcgaatg tctctcaccg cccagggcgc
    23281 aagccagcca ctggttcgcg ccgacaacat gcgtctggac gtggcgcttt taccactact
    23341 gagtcatcaa ctgagcgtta agcaggtgat gctaaaaggg gcagtgatcc aactgacgcc
    23401 gcagacggaa gcggtgcgca gtgaagacgc tccggttgca ccgcgcgaca ataccttgcc
    23461 ggatctgtca gacgatcgcg gatggtcgtt tgatatatcc agtcttaagg tggcggacag
    23521 cgtgctggtg ttccagcatg aagatgacga gcaggtgaca atccgcaata tccgcctgca
    23581 aatggaacaa gatccccaac atcgtggctc atttgagttc tccgggcggg ttaatcgcga
    23641 tcagcgcgat ctcacaatat cccttaacgg tacggtggat gcttctgatt atccgcatga
    23701 tttaacggcg gctattgaac aaattaactg gcagttgcag ggtgccgatt taccaaaaca
    23761 aggtattcag gggcagggga gtttccaggc ccagtggcag gagtcacata aacgcctttc
    23821 atttaaccaa atcagcttga ccgccaatga cagtacgctg agcgggcaag cacaggtcac
    23881 gctgacagag aaaccggaat ggcagctgag actgcaattc ccgcaactga atcttgacaa
    23941 cctcatcccg cttaatgaaa ccgcgaatgg tgaaaacggt gccgcgcagc aggggcagag
    24001 ccaatcaacg ttgccgcgcc cggtcatttc ttcgcgtatt gatgaaccgg cctatcaggg
    24061 actgcaaggc tttacggctg atattttgtt gcaggccagt aacgtgcgct ggcgcggaat
    24121 gaattttaca gatgttgcca cgcaaatgac caacaagtcg ggtttgctgg aaattactca
    24181 actgcagggc aaacttaacg gtggacaggt ttcactgccg ggcacgctgg acgcgacatc
    24241 aataaatccg cggataaact tccagccacg gctggaaaac gttgagattg gtaccattct
    24301 gaaggcgttt aactatccga tttcgttgac cggaaaaatg tcactggctg gtgatttctc
    24361 cggtgctgac atagatgccg acgcattccg ccacaactgg caaggacagg cacatgtcga
    24421 aatgaccgac acgcgcatgg aagggatgaa cttccagcag atgattcagc aagcggtaga
    24481 gcgtaatggc ggtgatgtga aggccgctga aaactttgat aacgtgacgc ggcttgaccg
    24541 ctttaccacc gatttgacgt tgaaggatgg cgtcgtgacg ttaaacgaca tgcaaggtca
    24601 atcgcctgtg ctggcgctga caggggaagg catgttgaat ctggcagatc aaacctgcga
    24661 tacccagttt gatattcggg tcgtgggtgg ctggaacggg gaaagtaaac tgattgattt
    24721 cctgaaagaa acgccagtac cgctgcgggt ttatggcaac tggcagcaac tcaattacag
    24781 cctgcaagtg gatcagttac tgcgcaaaca tctacaggac gaagcgaaac gtcgcctgaa
    24841 tgactgggcc gagcggaata aagattcccg caatggcaag gatgtgaaga agttgctgga
    24901 gaagatgtag gttaccaggt gaccagtcct gtaggcctga taagacgcgt aaagcgtcgc
    24961 atcaggcgtt cgtggcggca tcattatatt ctgaatatgt aagatcgaag gctgttccgt
    25021 tcacttgatg ttcctcgcta ctatggaacg ctcaagccgg tgaacgtgcc agggtggctc
    25081 aatcgccacc accctggcaa cccgggctcc cggcggtaaa tcgccgctgg cgcggttccc
    25141 tcatcttatt cctttcggct attgggtaaa atgaccgata ccatcacacc tcgtactcca
    25201 actcgccatc cttatgcagc ggaataatct gcaccttctg cacgcgatgg ctttccacct
    25261 gcaacgtttt aagcaaataa tcccccacct gaacttcttc gccaggtttt ggaatacgct
    25321 gtaaatattc catcaacagc ccggcaatgg tgtgatattc acgtttttca tccagcggca
    25381 gcggcacata ttgcaccaga tcttccagcg gcatatgacc attcgccgtc caggaaccgt
    25441 ccgcattctt ctgaatatca tggcgggcgt cgatctcttc cacttcgttc ggtaagttac
    25501 cgacaatggt ttcagtgacg tcacttaatg tcacaatccc ttccaccgag ccaaactcat
    25561 ccaccacaaa agcaaagtgc gtgcgggcat tacggaactg ctccagggca ggtaacaacg
    25621 gcaaggtttc cgggaacacc agcggctggc gaatcaacac ccgcaggttg agcggttcgc
    25681 cgcgcagaga ctgttgtagc aggtcgataa cgtgaacaac accgagcaaa tcttctgcgt
    25741 catcaccgtc ggtaaccacc agccgcgtat gctgatttcg ctccagcaac tggcggattt
    25801 cttcttccgg cgcgttgaga tcgatatgct caatatcgtg gcgcgacgtc ataatgctgc
    25861 tgacggtacg ctggttaaga ttaagtaccc gctcaatcat ccgccgttcc tgcggattaa
    25921 agatctgctg gttaccatga tccaccagca tggacgcggt ttcggcgtcc agttccgcat
    25981 cttctttttg cccgctaagc aggcgcatta ccgcttcggt agtccgctgg cgcagcgtct
    26041 ggttagcgga aagaaaacgt cgccggttaa agatagccaa ctgattgagc gcctcgatca
    26101 tcaccgagaa accaatggca gcgtacaggt agcctttcgg aatgacgaag ccgaaacctt
    26161 ctgccaccag gctaaagcca atcatcaaca ggaagcttaa gcagagaata acgatcgtcg
    26221 ggtgactgtt aacaaattgc gttaacggct tgctggccat caacatcagg ctgatagcga
    26281 taaccacggc ggccatcatg accagtaaat ggtcgaccat cccgacggcg gtaatcactg
    26341 agtcgagtga gaaaatggcg tccagtacca caatttgcgt caccacgccc cagaacttcg
    26401 cacctttgcg ttgtgtggga ttattgctgt ctttcccttc cagccgttcg ttcagctcca
    26461 tcgtggcttt gaacaacagg aagaaaccac caaacagcat gattaagtcg cgggcgctaa
    26521 aggtaaacga gcggaagctg aacagcggtt gagtcagggt gaccagccag gagattgacg
    26581 ccagcagtaa caggcgcatt aacatcgcca gcagcagccc ggtaacccgt gcgcggtcgc
    26641 gctgcctcgg cggtagtttt tcggcgagga tggcaataaa gaccaggtta tcaatgccga
    26701 ggaccagttc gatcacaatc agcgtgatta acccggccca gatagacgga tcggcaatcc
    26761 attccatagt aagtacaaaa cctattcgtt gtatgacaaa tgtcacaatt cgatcatgga
    26821 taaatcgggg caaaaatgca atgttgcagc actatttatc tcatattatt tgagggtaat
    26881 aacctcacat tatccctgaa ttaaaagtg
//
LOCUS       DAZOZY010000037        26893 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZOZY010000037 DAZOZY010000000
VERSION     DAZOZY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26893
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.037"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..807
                     /locus_tag="ACHURQ_001353"
     CDS             <1..807
                     /locus_tag="ACHURQ_001353"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFR0823179.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            963..2498
                     /gene="gadC"
                     /locus_tag="ACHURQ_001354"
     CDS             963..2498
                     /gene="gadC"
                     /locus_tag="ACHURQ_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR0823180.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2629..3948
                     /locus_tag="ACHURQ_001355"
     CDS             2629..3948
                     /locus_tag="ACHURQ_001355"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR0823181.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4320..5702
                     /gene="dosC"
                     /locus_tag="ACHURQ_001356"
     CDS             4320..5702
                     /gene="dosC"
                     /locus_tag="ACHURQ_001356"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR0823182.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5727..8126
                     /gene="dosP"
                     /locus_tag="ACHURQ_001357"
                     /gene_synonym="pdeO"
     CDS             5727..8126
                     /gene="dosP"
                     /locus_tag="ACHURQ_001357"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR0823183.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8385..8966
                     /gene="ddpX"
                     /locus_tag="ACHURQ_001358"
     CDS             8385..8966
                     /gene="ddpX"
                     /locus_tag="ACHURQ_001358"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR0823184.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8980..10530
                     /locus_tag="ACHURQ_001359"
     CDS             8980..10530
                     /locus_tag="ACHURQ_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0823185.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10532..11554
                     /locus_tag="ACHURQ_001360"
     CDS             10532..11554
                     /locus_tag="ACHURQ_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0823186.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11551..12447
                     /gene="ddpC"
                     /locus_tag="ACHURQ_001361"
     CDS             11551..12447
                     /gene="ddpC"
                     /locus_tag="ACHURQ_001361"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR0823187.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12444..13430
                     /locus_tag="ACHURQ_001362"
     CDS             12444..13430
                     /locus_tag="ACHURQ_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0823188.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13459..14355
                     /locus_tag="ACHURQ_001363"
     CDS             13459..14355
                     /locus_tag="ACHURQ_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR0823189.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="ACHURQ_001364"
     CDS             complement(14411..14842)
                     /gene="osmC"
                     /locus_tag="ACHURQ_001364"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR0823190.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15187..15402
                     /gene="bdm"
                     /locus_tag="ACHURQ_001365"
     CDS             15187..15402
                     /gene="bdm"
                     /locus_tag="ACHURQ_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR0823191.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15504..15641
                     /gene="sra"
                     /locus_tag="ACHURQ_001366"
     CDS             15504..15641
                     /gene="sra"
                     /locus_tag="ACHURQ_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR0823192.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15798..17495
                     /gene="maeA"
                     /locus_tag="ACHURQ_001367"
     CDS             15798..17495
                     /gene="maeA"
                     /locus_tag="ACHURQ_001367"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0823193.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17629..18631
                     /gene="adhP"
                     /locus_tag="ACHURQ_001368"
                     /pseudo
     CDS             17629..18631
                     /gene="adhP"
                     /locus_tag="ACHURQ_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            18777..19061
                     /locus_tag="ACHURQ_001369"
     CDS             18777..19061
                     /locus_tag="ACHURQ_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0823194.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="ACHURQ_001370"
     CDS             complement(19289..19942)
                     /gene="fdnI"
                     /locus_tag="ACHURQ_001370"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR0823195.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="ACHURQ_001371"
     CDS             complement(19935..20819)
                     /gene="fdnH"
                     /locus_tag="ACHURQ_001371"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR0823196.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="ACHURQ_001372"
     CDS             complement(20832..23879)
                     /gene="fdnG"
                     /locus_tag="ACHURQ_001372"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23292..23294),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0823197.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24111..24992
                     /gene="yddG"
                     /locus_tag="ACHURQ_001373"
     CDS             24111..24992
                     /gene="yddG"
                     /locus_tag="ACHURQ_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR0823198.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            25251..26351
                     /gene="ompC"
                     /locus_tag="ACHURQ_001374"
     CDS             25251..26351
                     /gene="ompC"
                     /locus_tag="ACHURQ_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0823199.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            26515..>26893
                     /locus_tag="ACHURQ_001375"
     CDS             26515..>26893
                     /locus_tag="ACHURQ_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0823200.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKES"
BASE COUNT         6679 a         6748 c         6718 g         6748 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagaggg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg actttgctga actgttgctg
      721 gaagactaca aagcctccct gaaatatctc agcgatcacc cgaaactgca gggtattgcc
      781 cagcagaaca gctttaaaca tacctgataa cgtttaacgg taacggtgtc ccgaaacgaa
      841 cccgtttcgg gataatttcc aaagtctgtt cactggcatt agcaacggaa aatattgttc
      901 tgaatacgct tcagaacaaa acaggtgcgg ttccgacagg aataccgttt tagggggata
      961 atatggctac atcagtacag acaggtaaag ctaagcagct cacattactc ggcttttttg
     1021 ccataacggc atcgatggta atggctgttt atgaataccc taccttcgca acatcgggct
     1081 tttcattagt cttcttcctg ctattaggcg ggattttatg gtttattccc gtgggacttt
     1141 gtgctgcgga aatggccacc gtcgacggct gggaagaagg tggtgtcttc gcctgggtat
     1201 caaatactct ggggccgaga tggggatttg cagcgatctc atttggctat ctgcaaatcg
     1261 ccattggttt tattccgatg ctctatttcg tgttaggggc actctcctac atcctgaaat
     1321 ggccagcgct gaatgaagac cccattacca aaactattgc agcactcatc attctttggg
     1381 cgctggcatt aacgcagttt ggtggcacga aatacacggc acgaattgct aaagttggct
     1441 tcttcgccgg tatcctgtta cctgcattta ttttgatcgc attagcggct atttatctgc
     1501 actccggtgc ccccgttgct atcgaaatgg attcaaagac cttcttccct gacttctcta
     1561 aagtgggcac cctggttgta tttgttgcct tcattttgag ttatatgggc gtagaagcat
     1621 ccgcaaccca cgtcaatgaa atgagcaacc cagggcgcga ctatccgttg gctatgttac
     1681 tgctgatggt agcggcaatc tgcttaagct ctgttggtgg tttgtctatt gcgatggtca
     1741 ttccgggtaa tgaaatcaac ctctccgcag gggtaatgca aacctttacc gttctgatgt
     1801 cccatgtggc accggaaatt gagtggacgg ttcgcgtgat ctccgcactg ctgttgctgg
     1861 gtgttctggc ggaaatcgcc tcctggattg ttggtccttc tcgcgggatg tatgtcacag
     1921 cgcagaaaaa cctgctgcca gcggcattcg ctaaaatgaa caaaaatggc gtaccggtaa
     1981 cgctggtcat ttcacagctg gtgattactt ctatcgcgtt gatcatcctc accaataccg
     2041 gtggcggtaa caacatgtcc ttcctgatcg cactggcgct gacggtggtg atttatctgt
     2101 gtgcttattt catgctgttt attggctaca ttgtgttggt tcttaaacat cctgacttaa
     2161 aacgcacatt taatatccct ggtggtaaag gggtgaaact ggtcgtggct attgtcggtc
     2221 tgctgacttc aattatggcg tttattgttt ccttcctgcc gccggataac atccagggtg
     2281 actctaccga tatgtatgtt gaattactgg tcgttagttt cctggtggta cttgccctgc
     2341 cctttattct ctatgctgtt catgatcgta aaggcaaagc gaataccggc gtcactctgg
     2401 agccaatcaa cagtcagaac gcaccaaaag gtcacttctt cctgcacccg cgtgcacgtt
     2461 caccacacta tattgtgatg aatgacaaga aacactaacc atgtctgaat ggttataaga
     2521 caagggagcg ataattcatc gctccctttt tcgtgctcgc tgtcgtattg acttaaccgg
     2581 gataagtacg agaatgagcg cacatctgtt taccggaaac cagcatatat ggatatctgc
     2641 tcccgaaacg agaaattagc gattagaaga ccagcgatac tggttgcact ggcactttta
     2701 ctgtgtagtt gtaaaagcac gcctccagag tccatggtga caccaccagc aggttcaaag
     2761 ccaccagcca cgacgcaaca atcgtcacaa ccgatgcgtg gcatctggct ggccacggtg
     2821 tctcgcctcg actggccacc ggtttcctcg gttaacatta gtaaccccac cagccgggcc
     2881 cgtgtacaac aacaggcgat gatcgacaaa ctggatcatc tgcaacgtct cggtataaac
     2941 acggtctttt tccaggttaa gccggacggt accgccctgt ggccatcgaa aattttgcca
     3001 tggtccgatc ttatgaccgg taagattggt gaaaatccgg gttacgatcc gctgcaattt
     3061 atgctcgacg aagcccacaa gcgtgggatg aaagtacacg cctggtttaa cccctatcgc
     3121 gtatcggtta atacgaagcc cggtactatc agggaactga atagcactct gtctcaacaa
     3181 ccggcgagcg tctatgtgca acaccgtgac tggatcagaa cgtctggcga tcgctttgtc
     3241 ctcgacccgg gcatccctga ggttcaggac tggatcacat caatagtcgc agaagtggtt
     3301 tcccgctatc cggtagatgg cgtgcagttt gacgactatt tctatactga gtcaccgggt
     3361 tcacggctaa atgataacga aacgtaccgc aaatacggag gcgcatttgc gtcaaaagca
     3421 gactggcggc gcaacaatac tcagcagtta attgcaaaag tatcgcacac cattaaaagc
     3481 attaagccgg gagtcgaatt tggcgttagc ccggcaggcg tgtggcgtaa ccgatcacac
     3541 gatccgctcg gttccgatac ccgaggcgcg gcagcctatg acgaatccta cgctgacacc
     3601 cgtcgatggg tggaacaagg attgctggat tacattgctc cccaaattta ctggccgttc
     3661 tcacggagtg ccgcgcgtta tgacgtgttg gcaaaatggt gggcggatgt cgttaaaccg
     3721 accaggaccc gcctgtatat cggtatcgcc ttctataaag tgggtgaacc ttcaaagata
     3781 gagccagact ggatgattaa cggcggcgta ccggaactga aaaagcagct cgatcttaac
     3841 gatgcggtac cagaaattag cggcaccatc ttgttccgtg aggactatct gaataaaccg
     3901 cagactcaac aagcggtcag ctatctgcaa agtcgttggg gcagttaaac tcccatgctt
     3961 actttgtaac aagccggtgc tttacccacc ggcttgttac accttgtgaa atatcccact
     4021 agtaatcatg cttacataag tcaaattaac cacgcaatta accacactta gttaaacagc
     4081 tataaatccc taataaaaat gtatgaatat gaaaaaccat acgatattcg ctgtcaccga
     4141 aaaataatta cgatgggcta tactcttctg aagaacagta gcgtagattg aaactgtcct
     4201 aagtcgctta tttcttttca gtatatcttc atatttcagg agaatatggt gcagttagtc
     4261 aatctcgcac gttgcgtttt gattccgtct gatttcccag ccttataagg gtgtgaatta
     4321 tggagatgta ttttaaaaga atgaaagatg agtggaccgg acttgtcgaa caggcagatc
     4381 cactcattcg tgctaaagcc gcggaaattg ccgttgcgca tgctcattat ctgagtatcg
     4441 agttttatcg aattgtccgc atcgacccgc atgccgaaga attcttgagt aatgaacaag
     4501 ttgagcggca gttgaaaagt gcgatggaac gctggattat taacgtgctt tctgcccagg
     4561 ttgacgatgt cgaaaggcta atacaaatcc agcataccgt cgcggaagtg catgcccgca
     4621 taggaattcc ggtagaaatt gtcgagatgg ggtttcgggt gctgaaaaaa atcctctatc
     4681 cggtaatctt ctcttcggat tattccgccg cagaaaaact tcaggtctat catttctcga
     4741 ttaacagtat tgatatcgcg atggaagtga tgacccgcgc gtttaccttt agtgacagta
     4801 gtgcctcaaa ggaagatgaa aattatcgta tcttctcgtt actggaaaac gccgaagaag
     4861 aaaaagaacg gcaaatagcc tcaatacttt catgggaaat agatattatc tataaaatcc
     4921 tgctggattc tgatttaggc agtagtttgc cattaagcca ggctgatttt ggcctgtggt
     4981 ttaaccataa aggtcgacat tattttagtg gcattgccga agtaggccat atctcccgtc
     5041 tcattcagga tttcgacggt attttcaatc aaaccatgcg taacaccagg aatttgaata
     5101 acagaagtct gcgggtgaaa tttttattac agataagaaa taccgtatcg cagattatta
     5161 ccttgctgcg tgaattgttt gaagaagtat cgcgccacga agtcggtatg gatgtactaa
     5221 cgaaattact taaccgccgt ttcctgccga ctatcttcaa acgcgaaatt gcccatgcca
     5281 accggaccgg tacaccgctg tcagtgctga ttattgacgt tgataaattc aaagagatca
     5341 acgatacgtg gggccataac actggcgatg aaattctgcg taaagtctct caggcctttt
     5401 atgacaacgt ccacagtagt gattatgttt ttcgctacgg gggcgatgaa tttatcattg
     5461 ttttgactga agcttctgaa aacgaaacgt tacgtaccgc agaacgtatt cgcagtcggg
     5521 tggagaaaac caaactgaaa gccgcaaacg gcgaggatat tgccctctca ctttccatcg
     5581 gtgccgccat gtttaatggt catcctgact atgagcgcct cattcaaata gccgatgaag
     5641 ctctgtatat cgccaaaaga cgaggtagaa accgtgttga actctggaaa gccagtcttt
     5701 agatgcgcca ggatgcagag gtaatcatga agctaaccga tgcggaaaat gccgccgatg
     5761 gcattttttt ccccgccctt gaacaaaata tgatgggcgc ggtgttaatt aacgaaaatg
     5821 atgaagtgat gtttttcaac cccgccgcag agaagctctg ggggtacaaa cgtgaagaag
     5881 tcattggcaa taacattgat atgctgattc cgcgggattt gcgtcctgcg catcctgaat
     5941 acattcgtca taaccgtgaa ggtggtaaag cgcgcgtcga ggggatgagt cgggagctgc
     6001 agctggagaa aaaagacggc agtaaaatct ggacccgttt tgcgctatcg aaagtgagcg
     6061 ccgaggggaa agtttattac ctggcgctgg tacgggatgc cagcgtagaa atggcgcaaa
     6121 aagaacagac ccgacaattg attattgccg ttgaccatct cgaccgaccg gtgattgtcc
     6181 tcgatccgga acgccatatt gtgcagtgca atcgcgcatt taccgaaatg tttggttact
     6241 gcattagcga agccagcggt atgcagcccg atacactcct gaacattcct gaattccctg
     6301 ccgataaccg cattcgttta caacagttgc tatggaaaac cgcccgcgat caggacgaat
     6361 ttctgctgtt gacacgcacc ggtgaaaaaa tctggattaa agcctctatc agcccggttt
     6421 atgacgtgct cgcgcatctg cagaacctgg taatgacctt ctcggatatc accgaagaac
     6481 ggcagatccg ccagcttgaa ggcaatattc tcgccgccat gtgcagcagc ccgccatttc
     6541 atgaaatggg ggaaatcatt tgtcgtaaca tcgaatctgt tctcaacgaa tcgcatgttt
     6601 cgctgttcgc actgcgcaac gggatgccga tacactgggc gtcatcttcg cacggtgcag
     6661 aagttcaaaa tgcgcaaagc tggtcagcga ccattcgtca gcgtgatggt gcgcctgcgg
     6721 ggatcctgca aattaaaacc tcgtcaggag cagaaaccag cgcctttatc gaacgcgtgg
     6781 cagatatcag ccagcatatg gccgcgctgg cgctggaaca ggaaaaaagc cgtcagcata
     6841 ttgaacaact catccaattt gatccgatga ccggtctgcc aaatcgcaat aacctgcaca
     6901 attacctcga tgaccttgtc gacaaagccg tctctccggt ggtgtatctc atcggtgttg
     6961 accatattca ggatgtgatt gatagccttg gctatgcgtg ggccgatcag gcattgctgg
     7021 aagtggtcaa tcgctttcgt gaaaaactca aaccggatca gtatctctgt cgtatcgaag
     7081 gtacgcagtt tgtcctcgtg agcctggaaa acgacgtcag taacattacc caaatcgccg
     7141 atgagctacg gaatgtggtc agcaagccga taatgattga cgataaacca ttcccgctaa
     7201 ccttgagtat tggcatcagc tacgacgtgg gtaaaaaccg cgattacctg ctctccactg
     7261 ctcacaatgc aatggattat attcgcaaga atggcggtaa cggctggcag ttcttcagcc
     7321 cggcgatgaa cgaaatggta aaagagcgtt tggttttagg cgcagcgctg aaagaagcga
     7381 ttagcaataa ccaactgaaa ctggtttacc agccgcaaat cttcgcagaa acgggtgaac
     7441 tgtacggcat cgaagccctt gctcgctggc acgatcccca gcatggtcat gtaccccctt
     7501 cacggtttat tcctctcgca gaagagattg gtgaaatcga aaatattggg cgctgggtca
     7561 tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca gaatattcat atcccggcgt
     7621 tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca gctgcctaat caggtgtctg
     7681 atgcaatgca agcctggggt attgacggcc accagctgac ggtagaaatc acggaaagca
     7741 tgatgatgga acacgatacc gaaatcttta agcgcattca gatcctgcgc gatatgggcg
     7801 tgggcttatc ggtagatgat tttggcacgg gcttttccgg attatcccgc ttagtcagtc
     7861 ttccggtaac ggaaatcaaa attgacaaaa gttttgtcga tcgttgtctg acggaaaaac
     7921 gcatccttgc cttacttgaa gccattacca gcattgggca aagcctcaat ttaaccgtcg
     7981 tggcggaagg cgtcgaaacc aaagagcaat ttgagatgct acgcaagatc cactgtcgcg
     8041 ttattcaggg atatttcttt tcccgccccc tacccgccga ggaaattcca ggctggatga
     8101 gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc ccgcactcgc gggttttcat
     8161 ttaacgtgac actgtcactt aaatactgtg atttcagcta cgattccggg agattccttc
     8221 cttaacttcc cctcttgcac caaaactgca ctacgctgca caaatgttga tcaaaagtta
     8281 aaaaatataa ctttaagcca gcattctggc gcttatcccg gcatggcatg agatctgcat
     8341 aagcggaaag cgcagcaatt tttgtcttaa tacaggaacc gtttatgtcg gataccaccg
     8401 aactggttga tttagccgtg atcttccctg atctggagat cgaattgaaa tacgcctgcg
     8461 ctgataacat cacaggtaaa gctatttacc agcaagcgcg ttgtctgtta cacaaggatg
     8521 cgattaccgc gctggcgaaa agtatcagta tcgcccagct gtcaggctta caactggtga
     8581 tttacgatgc gtatcgccca caacaagcac aggcgatgtt gtggcaagcc tgcccagacc
     8641 cgcaatatgt tgttgatgtg acggtcggtt ctaatcacag ccgtggcacg gcgatcgacc
     8701 tgacgcttcg tgatgagcac gggaacatcc tggatatggg cgcagggttc gatgaaatgc
     8761 acgagcgctc ccatgcctat cacccttccg tcccgcccgc cgctcagcgc aatcgcctgt
     8821 tgctgaatgc gataatgact ggtggcggtt ttgtcggtat ctccagcgaa tggtggcact
     8881 tcgaattacc ccaggcagcg agttaccctc tgctcgctga tcaattctct tgttttatat
     8941 cccctggcac acagcacgtc agttaatcag gaaactgtca tgaagagatc gatatcgttt
     9001 cgtcccacat tgctcgcgct cgtccttgcc acaactttcc cggttgcgca cgccgccgta
     9061 ccgaaagata tgctggtgat tggtaaggcc gccgatccac aaaccctcga cccggcggta
     9121 acaatagata ataacgactg gacagtgacc tacccgtctt atcagcggct ggttcagtac
     9181 aaaacggacg gtgataaagg ctcaaccgac gttgaaggcg atctggcaag tagctggaaa
     9241 gcgtctgacg atcaaaaaga gtggacgttc accctgaagg acaatgctaa atttgccgat
     9301 ggcacacctg tcactgccga agcagtaaaa ctctcttttg agcggttact aaaaatcggc
     9361 caggggccag cagaagcatt tcccaaagat ttaaagattg atactcccga cgaacatacg
     9421 gtgaagttta cccttagcca accattcgca ccgttcctct acacgctagc gaatgacggt
     9481 gcatccatta tcaatccggc ggtcttaaag gaacatgcag cggatgatgc tcgcggcttc
     9541 ctcgcgcaaa ataccgccgg ttccggacca tttatgctga aaagctggca aaaaggtcag
     9601 caattagttc tggtgccaaa tccgcattac cccggcaata aaccgaactt taagcgagta
     9661 tcggtaaaaa ttatcggtga aagtgcctcc cgtcgcctgc agctctcccg tggcgacatt
     9721 gacattgccg atgcgctgcc ggtggatcaa ctcaacgccc tgaagcagga aaataaagtc
     9781 aatgtggcag agtatccgtc actgcgcgtt acctatctgt atctgaataa cagcaaagcg
     9841 cctcttaatc aggcggatct gcgtcgggcc atttcctggt ctaccgatta tcagggcatg
     9901 gttaacggca ttctgagtgg taacggaaaa cagatgcgcg gcccgattcc ggaaggcatg
     9961 tggggctacg atgcgacggc aatgcaatac aaccatgacg aaacgaaagc caaagccgaa
    10021 tgggataaag tgacgagcaa acccaccagc ctgacgtttc tctactccga taacgatccg
    10081 aactgggagc ctattgctct ggcgacacaa tccagtctca acaagctggg catcaatgtg
    10141 aagctggaaa agctggcgaa cgccaccatg cgcgacagag tgggtaaagg tgattacgac
    10201 attgcgattg gcaactggag tccggatttt gccgacccgt atatgtttat gaattactgg
    10261 tttgagtcag acaaaaaagg tctgccgggt aaccgctcgt tctatgaaaa cagtgaggtc
    10321 gataagttac tgcgcaatgc gcttgcgacc accgaccaga cgcagcgtac ccgggactac
    10381 cagcaggcac agaaaatcgt cattgatgac gctgcttatg tgtacctgtt ccagaaaaac
    10441 taccaactgg cgatgaacaa agaggtgaaa ggctttgtgt tcaatcccat gctggaacag
    10501 gtcttcaata tcaataccat gagtaaataa cgtgaccttc tggagtattt tacgccaacg
    10561 ctgctggggg ctggtgctcg tggtggcggg cgtctgcgtg attacgttta ttatctcgca
    10621 tctgatccct ggcgatccgg cgcggttact ggcgggtgac cgcgccagcg atgctatcgt
    10681 ggaaaatatt cgccagcaac tgggactgga ccagccactg tatgtacagt tttaccgcta
    10741 cttcagcgac ctgtttcatg gtgacctggg aacatccatt cgtaccgggc gtccggtgct
    10801 ggaagagttg cgtatatttt tcccggcgac gctggaactg gctttttgtg ccctgctgct
    10861 ggcactcctg attggcatcc cgctgggcat actctctgca gtctggcgaa atcgctggct
    10921 ggatcatctg gtgcgaataa tggccattac cggaatctcc acacctgcgt tctggcttgg
    10981 actgggcgtc attgtgctgt tttatggtca tctgcaaatt cttcccggcg gcggaaggct
    11041 tgatgactgg ctggatccac caacgcacgt taccggcttt tatctgctcg atgcgctgct
    11101 tgaaggcaac ggtgaagtct tcttcaatgc gttgcaacat ctcatcttac cggcattaac
    11161 gctggcgttc gttcacctgg gaattgtcgc tcgccagatc cgctcagcga tgctggaaca
    11221 attgagtgaa gactacattc gtaccgcccg ggccagcggc ttgcccggct ggtatatcgt
    11281 tttatgttat gcgctaccca atgcgttgat cccatcgatt accgtattgg gtctggcgct
    11341 gggcgatttg ttgtatggcg cagtgctcac cgaaaccgtt tttgcctggc ccggaatggg
    11401 tgcatgggta gtaacatcaa tacaggcgct cgacttcccg gcagtgatgg gctttgccgt
    11461 cgtggtttca tttgcttatg tgctggtcaa cctggtggtg gatttgctct atttgtggat
    11521 tgatccgcgt atcggacgtg gaggtggtga atgatgctaa gcgaggaaac gtccgccgta
    11581 cgcccacaaa aacaaacgcg atttaacggt gcaaaactgg tatggatgct gaaaggcagt
    11641 ccgctcaccg tgaccggcgc agtcatcatt gtattaatgc tattgatgat gattttttca
    11701 ccgtggcttg cgacgcatga tcccaacgcc attgatttaa ccgcccgcct tttgccgcct
    11761 tctgcggcgc actggtttgg caccgatgaa gtgggacgcg atctgttcag ccgcgtactg
    11821 gtcggcagtc agcaatcaat tctcgccgga ttagtcgtgg ttgccattgc gggtatgatt
    11881 ggttcgctac tcggatgtct atccggtgtg cttggcggac gcgcagacgc catcatcatg
    11941 cgcatcatgg acattatgct gtcgattcct tcgttggtac tgacaatggc actggctgcc
    12001 gctctcgggc cgagtttatt taacgccatg ctggcgattg ctattgtgcg aattcccttt
    12061 tatgtgcgcc tggcgcgggg gcaagcatta gttgtacgcc agtataccta tgttcaggcg
    12121 gcgaaaacct ttggtgcgtc gcgttggcat ctgatcagct ggcatatttt acgtaactcc
    12181 ctgccgccgc tgatcgtgca ggcatcgctg gatatcggta gcgcgatttt aatggccgcc
    12241 acgttgggat ttattggcct gggtgctcaa caaccgagtg ctgaatgggg cgcgatggta
    12301 gctaatggtc gcaactatgt actcgatcag tggtggtatt gcgcatttcc gggggcagcg
    12361 attttgctta ccgccgtcgg gtttaatctc tttggtgatg gtattcgcga tctgcttgac
    12421 ccgaaagcag gaggaaagca gtcatgaccc aacccgttct ggacattcaa cagctgcatt
    12481 tgagtttccc cggttttaac ggttatgttc acgtgctcaa caatgtgtcc ttgcagatta
    12541 accgcggtga aattgtcggt ctggtgggag aatccggctc aggtaaatca gtcaccgcaa
    12601 tgctgattat gcgtctgcta ccgacgggca gttattgcgt acatcggggg cagatttcac
    12661 tgctgggaga cgatgttctt aacgctcggg agaaacagct tcgtcagtgg cgcggcgcac
    12721 gagtggcgat gatctttcag gaaccgatga ccgccctcaa tccgacacgt cgaataggtc
    12781 ttcagatgat ggacgtgatc cgccatcatc aaccaataag tcgtcgggaa gccagagcta
    12841 aagcgattac cctgctggaa gagatgcaaa tcccggatgc cgtggaagtt atgtcgcgct
    12901 atccgtttga gctttcaggt ggtatgcgcc agcgggtaat gattgcgctg gcattctcct
    12961 gcgagccgca attgattatt gccgacgaac cgactacggc gctggacgtc acggtacagt
    13021 tgcaggtact gcgtttgctt aaacataaag ctcgcgccag tggaactgcg gtactgttca
    13081 tcagccatga tatggccgtg gtgtcgcaac tgtgcgatag cgtttacgtg atgtatgccg
    13141 gaagcgtaat tgaaagcggc gtgacggcag acgttatcca tcatccccgg catccgtata
    13201 ccattggttt gctgcaatgc gcaccagaac atggagtacc acgccagcca ttacccgcca
    13261 ttccagggac ggtaccaaac ctcacccatt tgcctgacgg ctgcgctttt cgcgatcgtt
    13321 gctatgcggc aggtgcacag tgtgaaaacg tcccggcgct gacagcgtgt ggtgacaaca
    13381 accagcgctg cgcctgttgg tatccgcagc aggaggtcat aagtatctga cacgttatta
    13441 acgttacgcg acgtccatat caatttcccg gcccgtaaaa gctggcttgg taaaactacg
    13501 gaacatgttc atgcgattaa tggtattgat ttacagatcc gccgtggtga aaccttaggg
    13561 atcgtcggtg agtcaggctg cggcaaaagc accctcgcac agcttttaat gggtatgctg
    13621 caaccgagcc acgggcaata catccgttca ggatcacaac gcattatgca gatggtgttt
    13681 caggacccgc tctcttcgct taatccgcgc ttatcggtgt ggcgcatcat cacagaaccg
    13741 ctctggatag ctaagcgtag tagtgaacaa cagcggcgag cgttggcaaa ggagttggct
    13801 gtgcaggtgg gtattcgtcc ggagtatctc gaccgcctgc ctcatgcgtt ctccggcggg
    13861 cagcggcaac gcatcgccat tgccattgcc agagcactct cttcgcagcc tgacgtgatt
    13921 gtgcttgatg agccaacctc ggcgctggat atctccgtgc aggcgcagat cctcaattta
    13981 ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg
    14041 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    14101 ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat
    14161 tccctccccg ccattgataa accgctggag gaagaatggg cattatgtaa aacggatctg
    14221 ccgggaaacc gcacgttgcc acaaggctgt tttttctacg aacgttgccc tctggcaacc
    14281 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc
    14341 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    14401 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    14461 aaaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    14521 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaatcg gcatccactt
    14581 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    14641 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    14701 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    14761 cgccactctg ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    14821 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    14881 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatacgg cgaaaatatt
    14941 acttctgcac ttaaattaaa ttcccacaat agataaccgg gaatatatat ctcaagagat
    15001 attgcatcaa tatcaatcaa ctcaccacaa taataataca tatcaatatc atggagttaa
    15061 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    15121 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    15181 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    15241 gccattgagc aaggccttcg ggcagagcaa ggtgttgtca ctgaagatga cattctcatg
    15301 gagctgacca aatgggtgga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    15361 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    15421 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    15481 agcaacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    15541 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg
    15601 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    15661 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    15721 tggggccgac gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc
    15781 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    15841 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    15901 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    15961 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    16021 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
    16081 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    16141 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata
    16201 tggacgatat tctgcaaaac gtaccgaacc ataatatcaa agtgattgtg gtgactgacg
    16261 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    16321 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    16381 tggatgtcgg aacgaacaac caacaactgc ttaacgatcc gctgtatatg ggctggcgta
    16441 atccgcgtat cactgacgac gaatactatg aattcgttga tgaatttatc caggctgtga
    16501 aacaacgctg gccagacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    16561 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
    16621 cggcggtaac agtcggcaca ctgatcgcag caagccgagc ggcaggtggt cagttaagcg
    16681 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    16741 tcgcccagac ccagcgcgaa ggattaagcg aggaagcggc gcggcagaaa gtctttatgg
    16801 tcgatcgctt tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac
    16861 tggtgcagaa acgcgaaaac ctcagtgact gggataccga cagcgatgtg ctgtcactgc
    16921 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    16981 tgtttacgga agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    17041 cgctgtctaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggaccg
    17101 aaggtaacgc gctggtcgcc acgggcagcc cgtttaatcc agtggtatgg aaagataaaa
    17161 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggggtta
    17221 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
    17281 cacagtattc gccgctggtg ctgaacggcg aaggtctggt actaccggaa ctaaaagata
    17341 ttcagaaagt ctcccgtgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    17401 tggcggtgaa aacctctgcc gaagctttgc aacaagccat tgacgataat ttctggcaag
    17461 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    17521 cgggcaattt tcctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    17581 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    17641 gttgttacga aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat
    17701 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    17761 aatggcgatt ttggtgacaa aaccgacgta attctgggcc atgaaggcat cggtgtggtg
    17821 gcagaagtgg gtccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    17881 ttctacgaag gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt
    17941 tcagttaaaa atgccggata cagcgttgat ggcggaagag tgcatcgtgg tcgccgatta
    18001 cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg
    18061 ggtcaccacc tacaaagccg ttaagctgtc aaaaattcgt cccgggcagt ggattgctat
    18121 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg tctttaacgc
    18181 caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggtgc
    18241 agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg
    18301 tggcgctcac tctgcggtgg taacagcagt agctaaagct gcgtttaact cggcagttga
    18361 tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct
    18421 ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg
    18481 ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
    18541 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
    18601 tggccgtatg gtgattgatt tccgtcacta agaggtcttt gctgcgactc ccatgttcgg
    18661 gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt tgcgtaggaa
    18721 ggaacaataa tgatagtgtt cccagagaag gaacgctact gagagtaaag acactaatga
    18781 aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac gaacttaatg
    18841 tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca agccgattgc
    18901 tgaccggaaa agcagctttg acgccagaaa tggcaattaa actttccgtg gtgatcggca
    18961 gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag gcagaaaaaa
    19021 cggtggatgt gtcgcgatta cgccgtctgg ttacgcaata aaaaatggca ccttataaag
    19081 cgccatttga aactgatgac aaacgcaaca ttgcctgatg cgctacgctt atcaggccta
    19141 cacgttcact tcaatctatt gtatttgaag ggttttcatg ccggataagg cattcacgac
    19201 gcatccggga tgaacaaagc ccacgttgtc tacaatatga aaatggcgcc ttttacagcg
    19261 ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt
    19321 ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca
    19381 ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga
    19441 tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa
    19501 caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca
    19561 gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact
    19621 taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc
    19681 acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca
    19741 gtgcgacgaa aatcgcaatg ccgaagaacg ggtgcaaaat gcgtcccatc tgcggcgtac
    19801 cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca
    19861 ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca
    19921 caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg
    19981 ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc
    20041 agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc
    20101 agaccgtaat acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca
    20161 ccgacccctt tcgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc
    20221 acgcgctctt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc
    20281 ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg
    20341 catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca
    20401 atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac
    20461 tgaatgattg caccagcaga cgggcacgcc ttcaggcagc cgggatcttc acagtgcata
    20521 cagccgtctt tacggatcag ccactccagc ttgccgttct gttcggtttc gctaaagcgc
    20581 atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc
    20641 cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg
    20701 atacaggtgg aaacgtcgat aagttttgcg acttctgctt tgtaatcacg cacctgagaa
    20761 ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc catagccatc
    20821 tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc
    20881 gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca
    20941 ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc
    21001 agcggtttca gacggcgcgt taccaccgcc accgcgcgga taaagccacg cttgcttgag
    21061 acagtgacac gatcgccatt attaatgcct ttcgccgccg ccagcgtttc gctgatttcc
    21121 acaaactgtt ccggctgagc aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag
    21181 tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc
    21241 agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg
    21301 ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt
    21361 ttgttgatgg caaacaggcg tcccatccct tccggctgca tgataaacgg cccggttggc
    21421 gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt gctgccgttc
    21481 cactggatca gcatccgttt cggatcccac ggtttaccat tgatatccgc cgaagcacgg
    21541 ttgtagagca cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga
    21601 ccggatgggt cggagttatc gcggttagcc atctggttgc cctgctctgt ccagctaccg
    21661 gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc
    21721 agcaactgac ctttcttcgc aatcagcacg ccattagcgt catagagatc ttccagcgca
    21781 tagccgttat tctctttcgc cacttcgtca gattgcggtt cgtgcggctg cttgtagttc
    21841 cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta cagctcgcgc
    21901 agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc
    21961 tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc atcttcttcg
    22021 gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg
    22081 ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc
    22141 accatgtact tcagcttgct caggcagctc accactttgt ttttgtccgg gaaggacgca
    22201 accgggttaa agccctggca gaagtaaccg gtgactttgc cttcgtccat catgttgaaa
    22261 tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc
    22321 tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag
    22381 ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac
    22441 gactgcaaat caacctgttt ttctgacgga agcgtcagat aacctggcag gctggtagag
    22501 agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc gttcacgccg
    22561 ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc
    22621 tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc
    22681 ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt tggcgtaccg
    22741 cagatgtttt caacgacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac
    22801 acacagcgcg gatgagtcag tgtttcatcg cgttttgcat agccgttttc atcgaactga
    22861 tagttccagg acgacttatc atactggcgt ttttcagcgt cgtagccgct gaacaggccg
    22921 tcttcgaaag caaaatcatc acgcaccagc aggctggcgt tggtgtaatg tttaacgtat
    22981 tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg
    23041 tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacga
    23101 ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa
    23161 ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg
    23221 atatccaccc agtggttggt catcgcaccg cgaccaaatg ttggagcaag acttgctacc
    23281 gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca
    23341 aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta
    23401 gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg
    23461 tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat
    23521 ttgtcagaac ctggcgcacg atattccggg tagcgcagac ggttttcgct gttgacgtaa
    23581 tccagcaaac cggccccttt tgggcacagc gcaccacggc ttaccggatg atccgggtca
    23641 ccttcaatgt gataaatcgc ttctctggcg tttttcgcgc catcacccag gctatacatc
    23701 aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat
    23761 aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc
    23821 gttgttccag ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt
    23881 gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc
    23941 tttttgcgcg aaatcaaaaa gaacacgtgt atgccacaaa ataatccgcc tgcgcaaatt
    24001 aacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg
    24061 ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac
    24121 aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga
    24181 ttcgcggtgt cagcgagggg ctcggtccgg tcggcggcgc agctgctatc tattcattaa
    24241 gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct
    24301 atttactcgc cgggagtctg ttattcgtca gctatgaaat ctgtctggcg ctttccttag
    24361 ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca
    24421 gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac
    24481 caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac
    24541 attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta
    24601 ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta
    24661 acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac tattttctca
    24721 cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat
    24781 ttaccttagg atttgcttat gctgcatgga atgtcggtat attgcatggc aatgtcacca
    24841 taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca gccgtgctgc
    24901 tcagcgcccc gctgtcgttc tcgttctggc aaggcgcgct aatggtctgc ggcggttccc
    24961 tgctctgctg gctggcgaca cgtcgtggtt aaaataattg ccgggtctac ggcccggcag
    25021 ttaatcagat aaattccgac aatattaaaa aataaatttc aaataccagt aattcacctt
    25081 atatctcatt tcgacttcga tcacatatcg ataaataata ttatttcatc tatttttctc
    25141 attgttttta atcaccattc tattttaaaa acagtatgtc atttataaat gacgccgttt
    25201 aaaattcgtt tagaaaatac gtttaacgat aattaaaagg attctgttaa atgaaattaa
    25261 aaatagttgc ggtggttgta actggtttgt tagctgcgaa cgtagcacac gctgccgaag
    25321 tctataacaa ggatggtaat aaactcgacc tttatggcaa ggttaccgct ctacgttatt
    25381 ttactgatga taagcgtgac gatggtgata aaacttatgc ccgtctcggc tttaaaggag
    25441 aaacgcaaat caatgatcaa atgattggtt ttggtcactg ggaatatgat tttaaaggct
    25501 ataacgatga agccaacggc tcgcgcggca acaagacccg tctggcctat gctggtttaa
    25561 aaattagtga atttggctct ctggactatg gtcgtaacta cggtgtcggc tacgacattg
    25621 gttcatggac cgatatgttg ccagaatttg gtggcgatac ctggagtcag aaagatgtct
    25681 tcatgacata ccgtaccacc ggtctagcaa cctatcgcaa ctacgatttc tttggcttaa
    25741 ttgaagggct gaactttgcc gcgcaatatc aaggcaaaaa tgaacgtact gacaacagtc
    25801 atctttatgg tgctgactac acgcgtgcca acggtgacgg tttcggtatc tcctcaactt
    25861 atgtttatga tggctttggt atcggtgcgg tatataccaa atccgatcgg acaaatgcgc
    25921 aggaaagagc cgctgctaat cctctcaatg cctccggtaa gaatgcagaa ctatgggcca
    25981 caggtataaa gtatgatgcc aacaacatct actttgcagc taattacgct gaaacattaa
    26041 acatgaccac ctatggggat ggttatatct ctaacaaagc acaaagtttt gaagtggtgg
    26101 cgcaatatca attcgacttc ggcttgcgcc catcactcgc ttacctgaaa tcgaaaggca
    26161 gagatctggg ccgctacggc gaccaggaca tgattgagta tatcgacgtt ggtgcgacgt
    26221 atttcttcaa caaaaatatg tcgacctatg ttgattataa aatcaacctg attgatgaaa
    26281 gcgactttac ccgtgccgta gatattcgga ccgataacat cgtcgctacg ggcattacct
    26341 atcagttcta acgtcctctc ctgaccagcc cttctctggg ctggtttttg atttaagtgc
    26401 tgctgtatga ctccttaagt aaaaatgagc aatttttgat atttctggtc attcacctca
    26461 attaatatta ttccatatcc tccggggaca tcttactata atatttattg atgagtgatt
    26521 atgaccaata ttaatacagc ttgtgtaaaa aataatgcca gttatcaact taataacgca
    26581 ttacctaaca aagaaaccat ctccagcaat ttttgtgaac gactagaaca atggggtaat
    26641 aaatcggtta ataatggtga agaaagagca attgccgtgg agcgaattaa agaagcttac
    26701 aattcgaaca tggcatctct tgatttgtca tatcttgatt tgagtgaatt accgcctatc
    26761 ccctcgacag ttaatacgtt aaatctggaa aacaattgtc taacttgtct tgacttagct
    26821 gacaatgcca gtctcgttaa tatcaacctc agcgttaata aaattaacac gataaccttt
    26881 ccaaaagaat caa
//
LOCUS       DAZOZY010000038        26348 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZOZY010000038 DAZOZY010000000
VERSION     DAZOZY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.038"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..116
                     /locus_tag="ACHURQ_001376"
     CDS             <1..116
                     /locus_tag="ACHURQ_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0823201.1"
                     /translation="LWSLLKSSQECPPGESKITNKCLHNSCIIKICPYGLK"
     gene            complement(219..1469)
                     /gene="icd"
                     /locus_tag="ACHURQ_001377"
     CDS             complement(219..1469)
                     /gene="icd"
                     /locus_tag="ACHURQ_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR0823202.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1641..2294
                     /gene="rluE"
                     /locus_tag="ACHURQ_001378"
     CDS             1641..2294
                     /gene="rluE"
                     /locus_tag="ACHURQ_001378"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR0823203.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2304..2765
                     /gene="nudJ"
                     /locus_tag="ACHURQ_001379"
     CDS             2304..2765
                     /gene="nudJ"
                     /locus_tag="ACHURQ_001379"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR0823204.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2819..3925
                     /gene="mnmA"
                     /locus_tag="ACHURQ_001380"
                     /gene_synonym="trmU"
     CDS             2819..3925
                     /gene="mnmA"
                     /locus_tag="ACHURQ_001380"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR0823205.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3961..4602
                     /gene="hflD"
                     /locus_tag="ACHURQ_001381"
     CDS             3961..4602
                     /gene="hflD"
                     /locus_tag="ACHURQ_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR0823206.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4606..5976
                     /gene="purB"
                     /locus_tag="ACHURQ_001382"
     CDS             4606..5976
                     /gene="purB"
                     /locus_tag="ACHURQ_001382"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR0823207.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6144..6815
                     /gene="phoP"
                     /locus_tag="ACHURQ_001383"
     CDS             6144..6815
                     /gene="phoP"
                     /locus_tag="ACHURQ_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR0823208.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6815..8275
                     /gene="phoQ"
                     /locus_tag="ACHURQ_001384"
     CDS             6815..8275
                     /gene="phoQ"
                     /locus_tag="ACHURQ_001384"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR0823209.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8351..9472
                     /gene="roxA"
                     /locus_tag="ACHURQ_001385"
                     /gene_synonym="ycfD"
     CDS             8351..9472
                     /gene="roxA"
                     /locus_tag="ACHURQ_001385"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR0823210.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9521..10747)
                     /gene="pepT"
                     /locus_tag="ACHURQ_001386"
     CDS             complement(9521..10747)
                     /gene="pepT"
                     /locus_tag="ACHURQ_001386"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR0823211.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10997..12133
                     /gene="potA"
                     /locus_tag="ACHURQ_001387"
     CDS             10997..12133
                     /gene="potA"
                     /locus_tag="ACHURQ_001387"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR0823212.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12117..12974
                     /gene="potB"
                     /locus_tag="ACHURQ_001388"
     CDS             12117..12974
                     /gene="potB"
                     /locus_tag="ACHURQ_001388"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR0823213.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12971..13765
                     /gene="potC"
                     /locus_tag="ACHURQ_001389"
     CDS             12971..13765
                     /gene="potC"
                     /locus_tag="ACHURQ_001389"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR0823214.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13762..14808
                     /gene="potD"
                     /locus_tag="ACHURQ_001390"
     CDS             13762..14808
                     /gene="potD"
                     /locus_tag="ACHURQ_001390"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR0823215.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14964..15785)
                     /gene="cobB"
                     /locus_tag="ACHURQ_001391"
     CDS             complement(14964..15785)
                     /gene="cobB"
                     /locus_tag="ACHURQ_001391"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR0823216.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15801..16712)
                     /gene="nagK"
                     /locus_tag="ACHURQ_001392"
     CDS             complement(15801..16712)
                     /gene="nagK"
                     /locus_tag="ACHURQ_001392"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR0823217.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16741..17985)
                     /gene="lolE"
                     /locus_tag="ACHURQ_001393"
     CDS             complement(16741..17985)
                     /gene="lolE"
                     /locus_tag="ACHURQ_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR0823218.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17985..18686)
                     /gene="lolD"
                     /locus_tag="ACHURQ_001394"
     CDS             complement(17985..18686)
                     /gene="lolD"
                     /locus_tag="ACHURQ_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR0823219.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18679..19878)
                     /gene="lolC"
                     /locus_tag="ACHURQ_001395"
     CDS             complement(18679..19878)
                     /gene="lolC"
                     /locus_tag="ACHURQ_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR0823220.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20140..21213
                     /locus_tag="ACHURQ_001396"
     CDS             20140..21213
                     /locus_tag="ACHURQ_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR0823221.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21341..24787
                     /gene="mfd"
                     /locus_tag="ACHURQ_001397"
     CDS             21341..24787
                     /gene="mfd"
                     /locus_tag="ACHURQ_001397"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR0823222.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24931..25887
                     /gene="ldtC"
                     /locus_tag="ACHURQ_001398"
                     /pseudo
     CDS             24931..25887
                     /gene="ldtC"
                     /locus_tag="ACHURQ_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25970..26227)
                     /gene="bhsA"
                     /locus_tag="ACHURQ_001399"
                     /gene_synonym="ycfR"
     CDS             complement(25970..26227)
                     /gene="bhsA"
                     /locus_tag="ACHURQ_001399"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR0823223.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6420 a         6843 c         6687 g         6398 t
ORIGIN      
        1 atttatggag tttactcaaa agcagtcaag agtgtcctcc aggagaaagc aaaataacaa
       61 ataaatgttt acataatagt tgcattataa aaatatgccc atatgggctc aagtaatggg
      121 cttcatataa atatgctcga tatgctatta ataatatcca cataaataaa acaacgggcg
      181 tgttatacgc ccgtttcaat atttaactca tggagagatt acatgttttc gatgatcgct
      241 tcaccaaact ctgaacattt cagcagctta gcgccttcca tcagacgttc aaagtcataa
      301 gttacggttt tcgcgttgat agcaccttcc atacctttaa caattaaatc agccgcttca
      361 gtccacccca tatggcgcag catcatctca gcggagagga tgatagagcc tggatttact
      421 ttgtcctgac cggcatattt cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg
      481 tcaccgatgt ttgcgccagg ggcgataccg ataccgccaa cctgcgctgc cagggcgtca
      541 gaaatgtagt caccgttcag gttcatacag gcgataacat catattcagc cggacgcagc
      601 aggatctgtt gcaggaatgc atcagcaatc acgtctttaa tgacgatctc tttgccggtg
      661 ttcgggtttt taactttcag ccacgggccg ccgtcgatca gttcaccgcc aaactcttca
      721 cgcgccagct ggtagcccca gtctttaaac gcgccttcgg tgaacttcat gatgttgcct
      781 ttgtgcacca gagtcacaga gtcacgatcg ttagcaattg cgtattcgat cgctgcacga
      841 accagacgtt tggtgccttc ttccgaacac ggcttaatac cgataccaca atgttccggg
      901 aagcgaattt tcttcacccc catctcttca cgcaggaatt taatcacttt ctcggcgtcg
      961 gcagagtctg ctttccattc gatacccgca taaatgtctt ccgagttttc acggaagata
     1021 accatatcgg tcagttcagg gtgtttaacc gggcttggag tgccctgata gtaacgtacc
     1081 ggacgcaggc agatgtagag atccagttcc tggcgcaggg caacgttcag agagcgaata
     1141 ccgccaccaa caggagtggt cagcggacct ttaatggcaa cgcgatattc acgaatcaga
     1201 tcaagggttt cagcaggcag ccagacatcc tgaccataaa cctgtgtgga tttttcaccg
     1261 gtgtaaattt ccatccagga gattttacgc tcgcctttat aggctttctc gactgcagcg
     1321 tcgaccactt tcagcatggc tggggttaca tctacaccga ttccatcacc ttcaatgtaa
     1381 gggataatcg gattttcagg aacgttgagt ttgccgtttt gcagggtgat cttcttgcct
     1441 tgtgccggaa caactacttt actttccatt cacctctcct tcgagcgcta ctggtttgct
     1501 cgtctgccac cacgttgcat atgcgtttgc gtcctgcgat acggatgctt tagagcaatt
     1561 ttttgttaat gatttgtaat tggcttgtca atgctaccct attgtttgcc gtcatgaaag
     1621 atgcgcgtta ttaggctata atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa
     1681 cttcttttag aaatcaccag gttaagcgat tcagctcgca acgttctact aggcgtaaac
     1741 ctgaaaacca gcccacgcgt gtgatcctgt tcaataaacc ctacgatgtt cttccgcagt
     1801 tcaccgatga agccggacgc aaaacattaa aagaattcat cccggttcag ggcgtttatg
     1861 cagcaggtcg tcttgaccgc gatagcgaag ggttactggt gctgaccaat aacggcgcgt
     1921 tgcaggcgcg tttaacccag ccgggcaaac gcaccgggaa aatctattat gtgcaggtgg
     1981 aaggtattcc cacacaagac gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg
     2041 gccctaccct gcccgccggc gcggagctgg ttgacgaacc agcgtggtta tggccgcgga
     2101 atccaccaat tcgtgaacgc aaaagtattc ccaccagctg gctgaagatc accttatatg
     2161 aaggacgtaa tcgccaggtg cgccgcatga ccgcccatgt tggtttcccc acgctgcgac
     2221 tgattcgtta tgcgatgggt gattacactt tggataacct tgccaatggc gaatggcgag
     2281 aagcaacaga ataaggatgc acaatgttta aaccgcacgt taccgttgct tgcgtagtgc
     2341 acgcagaggg taaattttta gtcgttgaag agacgattaa tggtaaagcg ttatggaacc
     2401 aacctgccgg gcatctggaa gccgatgaaa ccttagtgga agctgccgcc cgtgagctat
     2461 gggaagaaac cggcatcagc gcgcaaccgc aacactttat tcgtatgcat cagtggattg
     2521 cgccagataa aacgccgttt ttgcgtttcc tctttgccat tgagcttgag caaatatgcc
     2581 cgacccagcc tcatgacagc gatatcgact gctgtcgttg ggtcagcgcc gaagaaattt
     2641 taaaggcgtc aaatcttcgt tcgccgctgg tggcggaaag tatccgttgt tatcaaagtg
     2701 ggcaacgcta tccgctggag atgattggcg attttaactg gccttttaca aagggtgtca
     2761 tctaaaaggc tgcgtgatag aatacgccgc cttgaagttc aatgtcgtga gtgatccaat
     2821 gtctgaaacc gcaaaaaaag taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc
     2881 tgcctggctg ttgcaacaac agggatatca ggttgaaggc ctgtttatga agaactggga
     2941 agaagacgac ggtgaggaat attgcacagc ggcagcggat ctggctgatg cccaggctgt
     3001 ctgcgacaag ctcggcattg aactgcacac cgttaacttt gctgccgagt actgggacaa
     3061 tgtcttcgaa ctgttccttg ccgaatataa agccggtcgc acgccgaatc cggatattct
     3121 gtgcaacaaa gagatcaaat ttaaagcttt cctcgaattt gccgccgaag atttaggtgc
     3181 cgattatatc gctaccggtc attacgtacg tcgggccgat gtcgatggca agagccgcct
     3241 gctgcgtggt ctggacagca ataaagacca gagctacttc ctttatacgc tcagccatga
     3301 gcagattgcg caaagcctgt tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat
     3361 tgctgaagat cttggtctgg tcaccgcgaa gaaaaaagac tctaccggca tttgcttcat
     3421 tggcgaacgt aaattccgcg agttcctggg ccgttatctc ccggggcaac cgggcaaaat
     3481 cattaccgtc gatggcgatg aaattggtga gcaccagggg ctgatgtatc acactctcgg
     3541 tcagcgtaaa ggtctgggta tcggtggcac caaagaaggc accgaagaac cttggtatgt
     3601 ggtggacaaa gacgtcgaaa acaacattct ggttgtcgct cagggccatg aacacccgcg
     3661 gctgatgtct gtcgggttga ttgcccagca gttgcactgg gtcgatcgcg aaccattcac
     3721 cggcactatg cgttgcacgg taaaaacccg ctatcgccag accgatatcc cttgcaccgt
     3781 caaggcgctg gacgatgatc gcattgaagt gattttcgat gaaccggttg ccgccgtgac
     3841 gccgggccag tctgccgtct tctataacgg tgaagtgtgc ctcggtggcg gtattattga
     3901 gcagcgtctg ccgctgccgg tctgattatt atctttactt aaaacaagga agcagtgaac
     3961 gtggcaaaga attactatga catcaccctc gccctggccg gtatttgtca gtcggcacgc
     4021 ctggtgcaac aactcgctca ccaggggcat tgtgatgccg atgcgctaca cgtctcactc
     4081 aacagtatta ttgatatgaa ccccagctcg acgctggcgg tttttggcgg tagcgaagcc
     4141 aacctgcgcg tcgggctgga aaccctgctc ggcgtgctca atgccagcag tcgccagggc
     4201 ttaaacgccg aattaacccg ctacacactc agcttgatgg tgcttgagcg caaactctcc
     4261 tcagcgaaag gcgcgctcga cactctgggc aaccggatca acggcctgca acgccagctc
     4321 gaacacttcg atttacagtc cgaaacgctg atgagcgcga tggctgctat ctatgttgat
     4381 gtgatcagcc cgcttggccc acgcattcag gtcaccggtt cccctgctgt actgcaaagc
     4441 ccacaagtgc aggcgaaagt tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc
     4501 tggcaccagg tcggcggcgg acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact
     4561 caggcaaaac aaattcttgc tcatttaacc ccggagttgt gatctatgga attatcctca
     4621 ctgaccgccg tttcccctgt cgatggacgc tacggcgata aagtcagcgc gctgcgcggg
     4681 attttcagcg aatatggttt gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa
     4741 aaactggccg cgcacgcagc gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt
     4801 taccttgatg caattgtcgc caatttcagc gaagaagatg ccgcacgcat caaaaccatc
     4861 gagcgtacca ctaaccacga cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg
     4921 gagatcccgg aactgcacgc ggtttctgaa ttcatccact ttgcctgtac ttcggaagat
     4981 atcaataacc tctcccacgc attaatgctg aaaaccgcgc gtgatgaagt gatcctgccg
     5041 tactggcgtc aactgattga tggcattaaa gatctcgccg ttcagtaccg cgatatcccg
     5101 ctgctgtctc gtacccacgg tcagccagcc acgccgtcaa ccatcggtaa agagatggca
     5161 aacgtcgcct accgtatgga gcgccagtac cgccagctta accaggtgga gatcctcggc
     5221 aaaatcaacg gcgcggtcgg taactataac gcccacatcg ccgcttaccc ggaagttgac
     5281 tggcatcagt tcagcgaaga gttcgtcacc tcgctgggta ttcaatggaa cccgtacact
     5341 acccagattg aaccgcacga ctacatcgcc gagctgtttg attgcgttgc acgcttcaac
     5401 accattctga tcgactttga tcgtgacgtc tggggttata tcgcccttaa ccacttcaaa
     5461 cagaaaacca ttgctggtga gattggttct tccaccatgc cgcataaagt taacccgatc
     5521 gacttcgaga actccgaagg gaatctgggc ctttccaacg cggtattaca gcatctggca
     5581 agcaaactgc cggtttcccg ctggcagcgt gacctgaccg actccaccgt gctgcgtaac
     5641 ctcggcgtgg gtatcggtta tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc
     5701 aaactggaag tgaaccgtga ccatctgctg gatgagctgg atcacaactg ggaagtgctg
     5761 gctgagccaa tccagacagt tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg
     5821 aaagagctga ctcgtggtaa gcgcgttgac gccgaaggca tgaagcagtt tatcgacggt
     5881 ctggcgttgc cagaagaaga gaaagcccgc ctgaaagcga tgacgccggc aaactacatt
     5941 ggtcgcgcca tcaccatggt tgatgagctg aaataaactc gtatcagtgc cggatggcga
     6001 tgctgtccgg cctgcttatt aagattatcc gctttttatt ttttcacttt acctcccctc
     6061 cccgctggtt tatttaatgt ttacccccat aaccacataa tcgcgttaca ctattttaat
     6121 aattaagaca gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac
     6181 gtcaccacct taaagttcag attcaggatg ctggtcatca ggtcgatgat gcagaagatg
     6241 ccaaagaagc cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg
     6301 gattgccaga cgaggacggt ctgtcactga ttcgccgctg gcgtagcaat gatgtttcac
     6361 tgccgattct ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg
     6421 ctggtgctga tgattacgtg actaaaccat ttcatattga agaggtgatg gcgcgaatgc
     6481 aggcattaat gcggcgtaat agcggtctgg cttcacaggt catttcgcta ccaccgtttc
     6541 aggttgatct ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt
     6601 tcgaatacac cattatggaa acgttgatac gcaataatgg caaagtggtg agcaaagatt
     6661 cgttaatgct ccaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac
     6721 tgatgggacg tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtg attactaccg
     6781 ttcgcggcca gggctatttg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt
     6841 cccgctctcg ctgcgggtac gttttctgtt ggcaacggct gcggtagtac tggtgctttc
     6901 gcttgcctac ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt
     6961 tcggctgtta cgtggcgaga gcaatctgtt ctataccctt gcgaagtggg aaaacaataa
     7021 gttgcatgtc gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta
     7081 tgatgagaac gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaagatgat
     7141 ccagcctgac tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac
     7201 cagcctcttg ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga
     7261 tgatgacgac gcggagatga cccactcggt ggcagtaaac gtctacccgg caacatcgcg
     7321 gatgccaaaa ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta
     7381 tatggtctgg agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct
     7441 gctgtgggtc gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg
     7501 cgaactggaa gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag
     7561 tctggtacga aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg
     7621 tacgacgctc accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac
     7681 gctgcgttct ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga
     7741 gcaaatcagc cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg
     7801 cgggacattg ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc
     7861 agcgctgaac aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga
     7921 gatcagcttt gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa
     7981 tgcctgtaaa tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct
     8041 ctatattgtg gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt
     8101 cgaccgtggt caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc
     8161 ccgcgaaatc accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg
     8221 tgcgcggatg gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg
     8281 tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata ccagcctgtg
     8341 gatgcttaac atggaatacc aactcactct taactggccc gattttcttg aacgtcactg
     8401 gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc cgatctctcc
     8461 agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg tcagtcacca
     8521 ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc tcggtgaaac
     8581 caactggtca ttactggtac aggcagtgaa ccactggcat gagccgaccg ccgcgctgat
     8641 gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt ctttttctgt
     8701 acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca ttcagggtac
     8761 cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact gcccacatcc
     8821 ggatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg agcctggtga
     8881 tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa atgcgatgaa
     8941 ctattccgtg ggctttcgcg cgccaaatac gcgggaactg attagtggat ttgccgatta
     9001 tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat cctgatgttc cacctcgcgc
     9061 tcatcctgcg gatgttctgc ctcaagagat ggataaactg cgtgagatga tgctcgaatt
     9121 gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc agtcacgtca
     9181 tgaactggat atcgcgccgc cagaaccgcc ttatcagcca gatgaaatct acgatgcgct
     9241 gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca ttggcgacga
     9301 cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg atgcactcgc
     9361 cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc cgtcattcct
     9421 cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt aagtttggat
     9481 ttaagccgga tgcggcatca catgccgcat cccttttcgc ttacttccgt tgcgccgtta
     9541 actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga gtcacaaact
     9601 catgcttacc atgatagttg taaccgccag tgaacaagtt cgggcacggt aatcccataa
     9661 acgacaactg cgcgccgtcg gtaccaccgc ggatcggttt cagttccggt tcaatatcgc
     9721 agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc actttctctc
     9781 gcatattgta gtaactgtct tcaatcacca gttcaatgta gcaatcagga tgtaaccctt
     9841 tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac tgtttacggt
     9901 cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc atgctcgcca
     9961 ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc ggaacttccg
    10021 catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc gtgcccggat
    10081 gaacattgtt accgacaatt ttgatattga ccgatgcggc gttgaagttt tcaaactcca
    10141 gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag gcatcaacat
    10201 caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg cgaatatcac
    10261 catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct gcaatacctg
    10321 ctttgtcatc ggcacctaac aaggttttac catcggtggt aatcaacgtc tgacccagta
    10381 gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata cccagcgcaa
    10441 tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg ctgcaatccg
    10501 gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg acgttagccg
    10561 gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc cccatttctt
    10621 cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg ggaacctgtc
    10681 tcacccctgc ttttgattgg gtatccagag atacgtagtt caaaaatcgc tcaagtagtt
    10741 tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag aaaagacaaa
    10801 tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat taccttaatg
    10861 aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg tttgccgtag
    10921 aattaccgcc ctcattaaga gtcaccaagg tggttaacca caaaccccgc atcggtaagc
    10981 catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga gttcgctttc
    11041 accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg tcattcccca
    11101 gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt ctggctgcgg
    11161 taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg gacgcatcat
    11221 gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga acactgtttt
    11281 ccaaagctac gcacttttcc cccacatgac cgtgttcgaa aatgtggcct ttgggttgcg
    11341 catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc tgcggatggt
    11401 gcaactggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc aacagcaacg
    11461 cgtcgccatt gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg atgagtcgct
    11521 ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag cgttacagcg
    11581 taagcttggt attaccttcg tctttgtcac tcacgatcag gaagaagcgc tcaccatgtc
    11641 agacaggatt gtggtgatgc gcgagggtcg tattgagcaa gatggtacac cgcgcgaaat
    11701 ttacgaagag ccgaaaaacc tgtttgttgc cggcttcatt ggcgaaatca atatgtttaa
    11761 cgccactgtc atcgaacgtc tcgacgagca gcgcgtacgc gccaacgttg aaggccgcga
    11821 atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg ttctgctgcg
    11881 cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag ggctgattgg
    11941 ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg aactggaaaa
    12001 tggcaagatg gtgatggtca gcgaattctt caatgaagac gaccctgact ttgaccactc
    12061 tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac tggctgatga
    12121 agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg cttgtgttgt
    12181 ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc gacgacgcca
    12241 gtttcgtcaa aatggtcttt acgctggata actacacgcg tctgctcgat ccgctctatt
    12301 ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc tgcctggtgc
    12361 tgggctaccc gtttgcctgg tttctggcga agctgccaca caaggtgcgt ccgctgctgc
    12421 tgtttctgct gattgttccc ttctggacca actcactgat tcgcatctac gggctgaaaa
    12481 ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc gttatcgaca
    12541 caccaattcg tatcatgttc acgcccagtg cggtgattat cggtctggtt tacattctgc
    12601 taccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag ccgttgcttg
    12661 aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgtatc attattccac
    12721 tgacgatgcc gggaattatt gccggatgtc tgctggtgat gctgccagcg atgggcctgt
    12781 tctatgtatc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac gtcatcaagg
    12841 tccagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt acgctgacta
    12901 tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtctgctg aataaaaagg
    12961 tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta tctacgcgta
    13021 cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc gctttggcat
    13081 caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg acagcctgtt
    13141 acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacatttg ctacgcttat
    13201 cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgtggtaagc cgttcgttag
    13261 cggaatgctg tttgtggtga tgatgtcacc agatatcgtg atggcgattt ctctgctggt
    13321 gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct cgcatatcac
    13381 cttctgcctg ccgtttgtgg tggtgacggt gtattcgcgc ctgaaaggtt ttgacgtgcg
    13441 gatgctggaa gcggcgaaag atctcggtgc cagtgaattt accattctgc gaaaaatcat
    13501 tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta ccctgtcgat
    13561 ggacgatgtg gtagtttctt cgttcgtcac cggaccaagt tatgaaattc tgccgttaaa
    13621 aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg caaccatatt
    13681 actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg ataaaacgaa
    13741 aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc ggcgggtgct
    13801 ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta tttctacaac
    13861 tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac cggtattaag
    13921 gctatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa aacgtacaaa
    13981 gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat gcgtaaagaa
    14041 gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct cgatccagac
    14101 atgctcaaca agccgtttga cccgaataac gactactcca ttccgtatat ctggggtgcg
    14161 acggcgattg gcgttaacgg tgatgcggtg gatcctaaat ctgtcaccag ttgggcagat
    14221 ctgtggaagc cagagtacaa aggtagcctg ctgctgaccg acgatgcccg tgaagtgttc
    14281 cagatggcgc tgcgcaaact gggctactcc ggtaacacca ccgatccgaa agagattgaa
    14341 gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa ctccgataac
    14401 ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa cggctctgct
    14461 ttcgttgcac gccaggcggg tacgccaatt gacgtggtgt ggccgaaaga aggcggcatt
    14521 ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg cgcgctgaaa
    14581 ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgccgaaac tatcggttat
    14641 ccaacgccaa accttgcggc gcgtaagctg ttaagcccgg aagtggcgaa cgataaaacg
    14701 ctctacccgg atgctgaaac cattaaaaat ggtgaatggc agaatgacgt tggcgcagcc
    14761 agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt cagcaaacgg
    14821 gcggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag tcaggacagt
    14881 aaagttttat cccgctcttt cgtcggtatt gtggtcccct tgtaggcctg ataagcgtag
    14941 cgcatcaggc aatgcttcct gctttataat cccttcagca acttttcaac aaactcaggc
    15001 accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccgac ctgacttggt
    15061 tcgagattca gttccacggt gtgcgcgcca tgcagtttcg cttcgtgaac aaacccagcc
    15121 gccggataaa catgcccgga agtaccaatg gcaatgaaaa tatcagccat cgacaacgcc
    15181 atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg cgggcgcaag
    15241 ggtgccggga actggcaaca atggcattta tcttctggcg taacgtctcc ggtccagtcg
    15301 agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat atgaatgaca
    15361 ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag caaaaagcga
    15421 tcgccgaggg cgtcttgcag tttagccagc gcaagatgcg cggcgttagg ctgaatttct
    15481 ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc aggatcgcga
    15541 tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag gccatctgcg
    15601 gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag tacgagtact
    15661 cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg ctgacgcaaa
    15721 cgctcgcgca ggcggcgttt atttttacga aaacgactta accgatgacc ccgacgcgac
    15781 agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc acgcattcct
    15841 cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg taagagatga
    15901 cgaggcagcc tgtccgccag ttgcgttgtg attgccggga aattcgataa gccaccacca
    15961 atgacgacca ggtcagagtc aacgatggtc aggatatttc ccagacaaac cgctaataaa
    16021 tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata aagcgcaata
    16081 atttcgggag cctgcaacgg ttgatgataa tagtgttgcc agagccacgc aaagccccga
    16141 cctgatagat aattttcgat acaaccaatt tgaccacagc cgcagcggcg taacgggaaa
    16201 tccagcccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa ctcgccggta
    16261 atgtagcttt taccggtaat cggtttaccg ttgaaaatca gcccgccgcc aacgccggtg
    16321 ccgagaatta accccatcac cagtggatat tgcgtaaatt catcatccca ggcttctgaa
    16381 agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg tgcgctcagg
    16441 tcggcacgca gcggtttacc gctggcagcg gggacattgg cggcatacag cgtaccatct
    16501 tctgtttccg gcattcccgg aataccgatg ccaacagaac ctttacagcc aaaacgttgg
    16561 tcagcttcgg ctaccagctc gcatactgca tctaaaaatg cgtcatagct gtcacgcggt
    16621 gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc aagcgcaatt
    16681 tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa tgtactgcct
    16741 ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg ccggatacca
    16801 acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt ccagccagtg
    16861 caattccgat ggcaggaagt caataaaata gatatcgctg gagaggaact gatgaccgat
    16921 cagcttttca atccgctcaa taatcggggt aagttgcagt gaaacaacta cgccgataat
    16981 cacaccacac aggctaccga acagccctgc cagcaatcca taccagacaa agatggcgcg
    17041 aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac tcttgtcttt
    17101 caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca ccagtaccat
    17161 cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc cgtaagtacc
    17221 aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc gtaccagctt
    17281 attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg aacccatatc
    17341 aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga gttgaccact
    17401 caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca gtttatgctc
    17461 gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt tcagcgcatc
    17521 cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc gccaggcatc
    17581 cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgcgggt taacgccctt
    17641 cacctgaatt gcgcgcagat tggtaccact ttctaccagt ccggtgaaat tgatatacgg
    17701 cgcagcggcg gcaatacccg gcactttttg cacgttatcc agtgcttcct gccagttagt
    17761 ccatggctga tttaccgcct caatttcacc gtgcggtacc acagcaagaa tgcggttatt
    17821 cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg ccacgccgag
    17881 ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc gtcgccgtcc
    17941 gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc cgcccccatc
    18001 aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct catacgtttc
    18061 gccagttgca ggtcgtgagt aaccaccagg aaggcggtgc cctgcaagcg attcaattcc
    18121 ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt aggctcatcc
    18181 gcaagtacca gacgcggatt attgaccagc gcacgggcaa tcgccacacg ctggcgttcg
    18241 ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac cgcttttaac
    18301 atctccagcg cacggctgtt gatttcagcg ggctttttct tgccaatcag cagtggcata
    18361 gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata aataaagccc
    18421 agcttctggt tgcgcagttc tgctttcgcc gccgaagaca gtttgctcat cggttgaccg
    18481 ttaaagatca catcgccgga ggttggtgta tccagcccgc ccagcaggtg cagcaaggta
    18541 cttttaccgg aaccagagct accgacgatc gccatcattt caccttcgcc gacgctgaaa
    18601 ctgacattgt gcagtacatc ggtttgcaca ctgccttcct gatagcgttt gcacaggttg
    18661 tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgag tggcggcagc
    18721 gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca gcgcaataac
    18781 aatgacctgt aaaggttcga tagcgaccgg cagcgccgcg ccatcaagca ggacgccgat
    18841 tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg ctccgaggat
    18901 cgcaccgata atcccggcgc tggccccttg caccataaag accatcatga tttgtcgcgg
    18961 agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatta ccatcagccc
    19021 tagtgaggta ataatgttaa acgccgcaac ggcgacaatc aggctcagca gcaagcccat
    19081 catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac gccagtcctg
    19141 ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactttca gcggctcatc
    19201 cagccacaaa cgccagccgg taatattgcc tgccggataa cgcatcagac gcgaggcatc
    19261 ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga aagtgccaat
    19321 cacattgaac aggcgctggc ttggaatacg ccccatcggc gtgaactggc tggcagatgg
    19381 taccatcacg cggatttgat caccgcgatt aacgcctagc tgtgaggcaa gttgttcgcc
    19441 gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga ccagatacgg
    19501 cgtaagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg ccacgctgcg
    19561 cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa cgccgtccag
    19621 tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag aagagagaat
    19681 tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc cgttcatcac
    19741 tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag aaagccagga
    19801 gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg taacgcaggc caataaatag
    19861 agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg agtatataag
    19921 cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa tctgaaaagc
    19981 ccccgtaaat caatcggcta aattgccatg ttttatcatc aatcttgccg cctgatctta
    20041 atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat aacccgcagg
    20101 atcaaccgca tgtaaattaa aaaagagcgt gttgtcctga tgaaacaaaa agagctatgg
    20161 attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc ggtcattacc
    20221 ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag caaatgctgg
    20281 atctatttca atctttacct tgcacccttt cgtatgccgg tttttttctt tatatccggc
    20341 tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga taaacggatc
    20401 tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc gttaagtgcg
    20461 ctgaaccagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc cgcttatgcc
    20521 gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt gtggtatctg
    20581 tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc gctgccacta
    20641 ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc gtggtgggga
    20701 atgaacagtg tgatccgcaa tttgccttat tacagccttg gcgcatggtt tggcgcaaca
    20761 ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc ttctttgctt
    20821 accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc gctggtatcg
    20881 attgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat gcgctccacc
    20941 agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca tcgcattctg
    21001 gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg gtcgccgcaa
    21061 gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg tactgttgcg
    21121 ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt ctccccgcct
    21181 tcactgcccg ctgccgtcag ttactctcgt taaaccatcg ccccagccgg ggcgattcaa
    21241 tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag atatgccccc
    21301 atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac aatatcgtta
    21361 tacactgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg gcgcagcctg
    21421 tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac tcattgcacc
    21481 agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg atcagatggt
    21541 gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc atcaggacat
    21601 tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg gcgtactgat
    21661 tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc acggtcatgc
    21721 gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc aactggacag
    21781 cgccggttat cgccatgttg accaggtgat ggagcacggc gaatacgcca cgcgcggcgc
    21841 gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt tctttgatga
    21901 tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg aggaagtaga
    21961 agcgatcaat ctgctgcccg cgcacgaatt tccgaccgat aaagcggcaa ttgaactgtt
    22021 ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata tttaccagca
    22081 agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccattgt tcttcagcga
    22141 accactgccg ccgctgttca gttatttccc tgccaatacc ttgctcgtga atactggcga
    22201 tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg agaatcgcgg
    22261 cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg tggacgagct
    22321 cttctcagag ctgaaaaact ggccccgggt gcagctaaaa actgaacatt taccgacaaa
    22381 agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc aggcacaaca
    22441 aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc cggtggtgtt
    22501 ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac gaattaaaat
    22561 tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt atctgatgat
    22621 tggcgctgcc gaacatggtt ttgtcgataa ggtgcgtaat ctggcgctga tctgcgaaag
    22681 cgatctgctc ggtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca ccatcaaccc
    22741 cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg tccatctgga
    22801 gcacggtgtc gggcgctatg ccggaatgac cacgctcgaa gcgggcggca ttactggcga
    22861 gtatttgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt cgtcactgca
    22921 tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata aacttggcgg
    22981 cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg tggcggcgga
    23041 attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta aacacgatcg
    23101 tgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg atcaggcgca
    23161 ggccattaat gcggtactta gcgacatgtg tcagccgctg gcaatggatc gtctggtgtg
    23221 cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc tggcagtaga
    23281 taaccacaag caggtggcgg tgttggtgcc taccaccctt ctcgcgcagc agcattacga
    23341 caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct cccgtttccg
    23401 cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa tcgatattct
    23461 gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag gcctgctgat
    23521 tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag cgatgcgcgc
    23581 gaacgtggat attctgacgc ttactgcaac gccgatccca cgcacgctga atatggcaat
    23641 gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc tggcagttaa
    23701 aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc gtgaaatttt
    23761 gcgcgggggg caggtttatt atctctacaa tgatgtggaa aacatccaga aagctgccga
    23821 acggctggca gaactggtgc cagaagcgcg gatcgccatc ggtcacgggc agatgcgcga
    23881 gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg tgctggtttg
    23941 tacaaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta tcattgaacg
    24001 cgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg gacgttcgca
    24061 tcatcaggca tatgcatggt tgctgacacc gcatccaaaa gcgatgacta ccgatgcaca
    24121 aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg cgctggcaac
    24181 gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa gcggctcaat
    24241 ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg atgcactgaa
    24301 agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag tcgagctgcg
    24361 gatgccgtcg ctattgccag atgatttcat tcctgacgtg aatacgcgtt tgtcgttcta
    24421 taaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag tcgagcttat
    24481 cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg cccgtctgcg
    24541 ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag gcggggtgat
    24601 cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc tgcaaaaaca
    24661 gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg atttgagtga
    24721 gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag agaacgcgat
    24781 cgcttaatgg taaatcgggg gcgtttctgc gcccccatct gcaacattta caaattcttt
    24841 gcacttccct gcactatccg acactgtcac catccataat tcaggcttat ctgtttatta
    24901 caataacctt atatttatta ttgatttttg gtgatgatca aaacgcgttt ttctcgctgg
    24961 ctaacgtttt ttacgttcgc cgctgccgtg gcgctaccgg caaaagccaa cacctagccg
    25021 ctgccgccag cgagcagtcg tctggttggc gaaaacaaat ttcatgtggt ggaaaatgac
    25081 ggtggttctc tggaagccat cgccaaaaaa tataacgtcg gctttctcgc tctgttacag
    25141 gctaaccccg gcgttgatcc ttacgtaccg cgcgcgggca gcgtgttaac gatcccgttg
    25201 caaaccctac ttccagacgc gccgcgcgaa ggcattgtga tcaacattgc ggagctgcgt
    25261 ctctattact acccgccggg taaaaattcg gtaaccgtgt atccaatagg tattggtcag
    25321 ttaggtggtg acacgctgac accgacaatg gtgaccaccg tttcagacaa acgtgcaaac
    25381 ccaacctgga cgccaacggc aaacatccgc gcccgttata aagcacaggg aattgagttg
    25441 cctacggtag tgccggctgg accggataac ccaatgggcc atcatgcgat tcgtctggcg
    25501 gcctatggcg gcgtttattt gcttcacggt acgaacgccg atttcggcat tggcatgcgg
    25561 gtaagttctg gctgtattcg tctgcgggat gacgatatca aaacactctt tagccaggtc
    25621 accccaggca ccaaagtgaa tatcatcaac actccgataa aagtctctgc cgaaccaaac
    25681 ggtgcgcgtc tggttgaagt acatcagccg ctgtcagaga agattgatga cgatccgcag
    25741 ctgctgccaa ttacgctgaa tagcgcaatg caatcattta aagatgcagc acaaactgac
    25801 gctgaagtga tgcaacatgt gatggatgtc cgttccggga tgccggtgga tgtccgccgt
    25861 catcaagtga gcccacaaac tctgtaaatc ctggataaaa aaaatccccg cgacacggca
    25921 tgtcgcgggg aaaaaatcag cagtagcagg cattaatgag ggttaatgct tatttataaa
    25981 ttactgctgt tccatggagg gtattcggac cggttacaga agtaatacgg aaagattttg
    26041 cgcccatctc atccgctttt tgcgccagct gctcttccag cgatcccaga tttgtccccg
    26101 cgttagcact gatagtaccg actttttgtt ggccttctgg cgttgactga acttcgacag
    26161 ccgcaaagtt ggcaaatgac atggagctta aaatcgccgc agcgatgagg gtttttacgt
    26221 ttttcataat agtggcctta tgcagatgaa tgacgtcgca agaaattact taacgatcga
    26281 taactaaatg atagatgtga tctggatcac atacaagata ttttttataa caatcattaa
    26341 ttaattta
//
LOCUS       DAZOZY010000039        25637 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZOZY010000039 DAZOZY010000000
VERSION     DAZOZY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.039"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHURQ_001400"
     CDS             complement(65..796)
                     /gene="rlmB"
                     /locus_tag="ACHURQ_001400"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR0823224.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHURQ_001401"
     CDS             complement(976..3417)
                     /gene="rnr"
                     /locus_tag="ACHURQ_001401"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR0823225.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHURQ_001402"
     CDS             complement(3456..3881)
                     /gene="nsrR"
                     /locus_tag="ACHURQ_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR0823226.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHURQ_001403"
     CDS             complement(4086..5384)
                     /gene="purA"
                     /locus_tag="ACHURQ_001403"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR0823227.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5488..5685)
                     /locus_tag="ACHURQ_001404"
     CDS             complement(5488..5685)
                     /locus_tag="ACHURQ_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR0823228.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHURQ_001405"
     CDS             complement(5767..6771)
                     /gene="hflC"
                     /locus_tag="ACHURQ_001405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR0823229.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHURQ_001406"
     CDS             complement(6774..8033)
                     /gene="hflK"
                     /locus_tag="ACHURQ_001406"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR0823230.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHURQ_001407"
     CDS             complement(8119..9399)
                     /gene="hflX"
                     /locus_tag="ACHURQ_001407"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR0823231.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHURQ_001408"
     CDS             complement(9476..9784)
                     /gene="hfq"
                     /locus_tag="ACHURQ_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR0823232.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHURQ_001409"
     CDS             complement(9870..10820)
                     /gene="miaA"
                     /locus_tag="ACHURQ_001409"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR0823233.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHURQ_001410"
     CDS             complement(10813..12660)
                     /gene="mutL"
                     /locus_tag="ACHURQ_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR0823234.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHURQ_001411"
     CDS             complement(12670..14007)
                     /gene="amiB"
                     /locus_tag="ACHURQ_001411"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR0823235.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHURQ_001412"
     CDS             complement(14026..14487)
                     /gene="tsaE"
                     /locus_tag="ACHURQ_001412"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR0823236.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHURQ_001413"
     CDS             complement(14459..16006)
                     /gene="nnr"
                     /locus_tag="ACHURQ_001413"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR0823237.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16005..17144
                     /gene="queG"
                     /locus_tag="ACHURQ_001414"
     CDS             16005..17144
                     /gene="queG"
                     /locus_tag="ACHURQ_001414"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR0823238.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17127..17180)
                     /locus_tag="ACHURQ_001415"
     CDS             complement(17127..17180)
                     /locus_tag="ACHURQ_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823239.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17414..17489)
                     /locus_tag="ACHURQ_001416"
     tRNA            complement(17414..17489)
                     /locus_tag="ACHURQ_001416"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17454..17456),aa:Gly,seq:gcc)
     gene            complement(17525..17600)
                     /locus_tag="ACHURQ_001417"
     tRNA            complement(17525..17600)
                     /locus_tag="ACHURQ_001417"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17565..17567),aa:Gly,seq:gcc)
     gene            complement(17758..17833)
                     /locus_tag="ACHURQ_001418"
     tRNA            complement(17758..17833)
                     /locus_tag="ACHURQ_001418"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17798..17800),aa:Gly,seq:gcc)
     gene            complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHURQ_001419"
     CDS             complement(18044..18589)
                     /gene="orn"
                     /locus_tag="ACHURQ_001419"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR0823240.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHURQ_001420"
     CDS             18684..19736
                     /gene="rsgA"
                     /locus_tag="ACHURQ_001420"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR0823241.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19833..20801
                     /gene="asd"
                     /locus_tag="ACHURQ_001421"
     CDS             19833..20801
                     /gene="asd"
                     /locus_tag="ACHURQ_001421"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR0823242.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20823..24146
                     /gene="mscM"
                     /locus_tag="ACHURQ_001422"
     CDS             20823..24146
                     /gene="mscM"
                     /locus_tag="ACHURQ_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR0823243.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHURQ_001423"
     CDS             complement(24297..>25637)
                     /gene="yjeM"
                     /locus_tag="ACHURQ_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0823244.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5928 a         7193 c         6501 g         6015 t
ORIGIN      
        1 tatcttacca gctatagaca aaaaaaccat ccaaatctgg atggcttttc ataattcaga
       61 cgaactagct gcgctgacga accgcttcaa ataagcaaat tccggttgca accgaaacgt
      121 tcagggaaga aacacttcct gccattggga tgctgatcaa ctcatcgcaa tgttcacggg
      181 tcaggcgacg cataccttca ccttccgcgc ccatcaccag cgccaagcgt ccggtcattt
      241 tgctctgata gagtgtatga tccgcctcgc ctgccgtacc gacgatccag atattctctt
      301 cctgcaacat acgcatggtg cgcgcaaggt tagtcacccg aatcagtgga acgctttctg
      361 ccgcgccgca ggctactttt ttcgccgtag cgttgagctg tgcggagcga tctttcggca
      421 caatcaccgc gtgaacgcca gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg
      481 gatcggttac accgtcgagg atcagcagga acggttgatc gagcgaagcg atcagatccg
      541 gcagatcgtt ttcctgatac tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca
      601 cggcaccgtc gcttttctcg tcgagatatt ggcggtttgc caactggata accacgccct
      661 gagactcaag ggcgtgaatc agcggtaaca gacgtttatc ttcacggcct tttaaaataa
      721 agacttcctg aaaacgttca ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt
      781 aaatcatttc gctcattaat gtactcgttg tttacgtttt gcctgatgca ctacgtttat
      841 ccggcctaca tgatctctgc aatatattga atttgcgtga ttatgtaggc cggataaggc
      901 gttcacgccg catccggcat gaacaaagcg cattttgtca gcaatctaac cctcttcttt
      961 taaagagggt attgatcact ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc
     1021 tgcggctatt ttctgcgttt tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc
     1081 cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa agtttacctt
     1141 tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt tcgctttctc
     1201 gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac taaagtcgat
     1261 tttgcgctcg tccatattaa ccgcttcgac gcgaacttcc acgcgatcgc ccaggcgata
     1321 agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc
     1381 attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca gacggacgaa
     1441 gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta cctggtcgag
     1501 catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc
     1561 cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat ggtagccgcc
     1621 ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatact taatggcgcg
     1681 gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat aggactgcaa
     1741 tgccaggcca aagtgaccac ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag
     1801 cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca gcagttccgc
     1861 gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct ccgccagcac
     1921 tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga acagtgccgg
     1981 ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca tgcactcttc
     2041 aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac ggcgttcagc
     2101 gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc
     2161 tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta
     2221 ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt aggtcagacg
     2281 cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga
     2341 aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg aacacaggcc
     2401 gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc
     2461 acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc tgacgtcggc
     2521 aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc
     2581 aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca aatcgacacg
     2641 gcccgctttt gcttcttccg gcacttcttc tttcagcccc gcaacctgtt gctcaacagc
     2701 ctgcggccag atatacggaa tttcatgggt acgcagagcg atatcaaccg ccatgccggt
     2761 gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc ggcgagtcgg
     2821 acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga tctgatcggg
     2881 cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc cgacgcccgc
     2941 ttcggtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc ggacaatacg
     3001 cgcttcacga cgacctttac ggtcagcacc cagcggctgc gccagcacct gatcgccatg
     3061 aatacaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc cttcaacccg
     3121 cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt cgaggcgttc
     3181 cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg
     3241 caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt ctaccgccag
     3301 ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa attcccgact
     3361 agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt gtgacatctc
     3421 ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa taatttataa
     3481 agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc cgtaagaaaa
     3541 ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac
     3601 tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc accaatacgt
     3661 atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac agcagtcacg
     3721 tagccggcac gactaagttg attgattatt ttgaccatat gattacggga gacgccgtag
     3781 acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc catgtagatc
     3841 agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc aaaagggaaa
     3901 tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc gctaatttat
     3961 ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc gtaatggctt
     4021 aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg ccaggcgtac
     4081 cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc cggaccggta
     4141 gagatgatat cgatcggcac accggtcagc tcttcaatac gcttgatgta gttcagcgcc
     4201 gcctgcggca ggccgctacg atctttcacg ccgaaagtgg attcagacca gcccggcatg
     4261 gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt ggtagccact
     4321 tcgcgaccat ccggcatacg gtaagccacg cagagtttaa cctctttcag gccatccaga
     4381 acgtccagct tggtcaggca gaagccagac agggagttca gctgtaccgc acgacgaacg
     4441 gcaacggtgt ccagccagcc ggtacgacga cgacgacccg tagttgcgcc gaattcgtta
     4501 ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa cggacctgca
     4561 cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata acgcgggccc
     4621 aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac gtacggataa
     4681 gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca
     4741 cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt caggatgtcg
     4801 gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc tttgtagtag
     4861 ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc gaaggtttct
     4921 ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc ataagcaggc
     4981 ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta
     5041 tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag cagacgctca
     5101 cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc ggccggagac
     5161 agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc tgatggaata
     5221 agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt gtgaccgccc
     5281 tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt acctttacct
     5341 tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca aaatcaccgt
     5401 ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt tgcactcaaa
     5461 aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca tgtagtagac
     5521 cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt
     5581 ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc
     5641 cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt atccttatag
     5701 aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg taccgcagtc
     5761 gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat cgctatccgg
     5821 gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac gcaggctacg
     5881 gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt tggctgcttc
     5941 ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca gcgttctggt
     6001 cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg
     6061 acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt cagacacttc
     6121 ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca gcgccgccat
     6181 actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc gctctgccgc
     6241 ttcggcaatg gcgttatctg ccgccggggt agtaacttca tcttctgtac ccgcagaacc
     6301 ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcgg tgacgatgtc
     6361 tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac gtttcaacag
     6421 cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc tgaaatcgct
     6481 gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg tcacaaagcg
     6541 gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa
     6601 cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat cgtcacgcag
     6661 taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa agacagacat
     6721 gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca tcgttattcc
     6781 ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat aatatcgccc
     6841 tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc tggcggcaga
     6901 cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc gcctttcagc
     6961 atctggtcta acggcagaac catcaggttg ccacctttat cgttaaccag cactttgcgg
     7021 gtgttaccca acactttttc catcgtctcg atatacagac gctcgcgagt aatttccggc
     7081 gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg agcttccagg
     7141 atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg accgttcgca
     7201 cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg ttcgttttca
     7261 cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg agctgcctgg
     7321 aagttgacgt ccagcagagt gatacccatg tcatacggac ggatagtctc ttccagttcg
     7381 cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc catggtgtat
     7441 ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg
     7501 gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt catctcaacg
     7561 cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc acgcacggct
     7621 tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag acccggctca
     7681 accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc tttaatggta
     7741 tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt aacgacacga
     7801 ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc ggtgccttta
     7861 ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc taaatcaggt
     7921 ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc aggtttgctg
     7981 cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc catgtttgtg
     8041 ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat atgactccac
     8101 gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt gtttacagag
     8161 gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt cctcctccat
     8221 ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgtc cttcctgcgg
     8281 cggcaaacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca aagcctgaaa
     8341 aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg gtttgttctc
     8401 ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt tcatcaccag
     8461 cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt
     8521 ttcttgtaca cgtacatccg ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt
     8581 ctcttgtaac gtggctttaa atgctgccac cagatcgtgc ggcaggtggc gaataaaccc
     8641 tacggtatct gcaagtacgg tttcaccgac atccgcgacg tcaatacgcc gcaacgtcgg
     8701 gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa
     8761 aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa cgtcggcttt
     8821 gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttctaggc gcgactgtat
     8881 ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt cacccggacc
     8941 acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt
     9001 agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg tacgcgcacg
     9061 ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact cgcacaaacg
     9121 ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga ccgaagcacc
     9181 cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa aatactttgg
     9241 gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg cggaagagac
     9301 cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat agatgtgtac
     9361 cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc tctaaataga
     9421 tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg aaaccttatt
     9481 cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca tgatggtagt
     9541 tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga gacgggacaa
     9601 cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac aggatcacga
     9661 actgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa taaatagaaa
     9721 ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat tgccccttag
     9781 ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt ctgaaaaaat
     9841 tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac aacctgtaat
     9901 acttcgtcac gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc ttcccaacca
     9961 cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc tcgataaacc
    10021 atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata acccacgcaa
    10081 cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg gacttctgct
    10141 tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg gagcagttca
    10201 cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc tcctgacgtt
    10261 tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc ccgggaaagc
    10321 ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc ctgaagttga
    10381 cgatgcaatg actcccaacc ttgctctgcc gcctgttgct caattctggc ccgcacttcc
    10441 gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata caacatcgta
    10501 ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc cgccagcgca
    10561 tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc cagcaatcgg
    10621 tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc catccctttg
    10681 taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg cagctcaatg
    10741 gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt aggcaggctc
    10801 gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat caacagattg
    10861 taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca cgtccgccag
    10921 cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat ttcgtgcaat
    10981 ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc ctatcagttc
    11041 aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc tgatggtcac
    11101 atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact gcgctttttc
    11161 taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaataagca gcggctgagc
    11221 gcaaactggc gcttcacccg gcgtcagttg tacctgacgc agccaacgtt ctgccactgg
    11281 caaggctaaa agtgaaatgt tgccgtcgcg ctccagcaac gcacagtcgg aatggacgat
    11341 agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct gcggttccgg
    11401 cgcttttaat ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca cttcaccttg
    11461 ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac tgcctgatgc
    11521 tggcgcagga gtgtagcgcg gagctaccgg ctcacgagct gccggttctg caaagtgatt
    11581 gcgccccgcc gccacgcggt tttccggaat ggaacgcggt gcaggttggg gttcatcgtc
    11641 cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct gatagataaa
    11701 atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt gcacgttgac
    11761 gtccacctga tgcggatcga tctccagata cagcacaaat gccggttgct gatcggcccc
    11821 cagtttgtct tcgcaggcct ggcggatcgc gtgattgatc aggcgatcgc gcatcatgcg
    11881 accgttcacg tagcaatact gaatttctgc cagtgcgggc gtggtgtgat ttggatcggc
    11941 cacccagccg cgtagcgtga gatcgccgtg ttgccattca atcgccagcg cttgttcaag
    12001 aaaagcggtg ccgcaaatcg cgcctaagcg ccgttctttt tgcccgcctt ccggcactgc
    12061 gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt cgaaacgcgc
    12121 cagcgcaatg cggcggatga tctcatcaat gtggttaaat tcggttttct cggtgcgcag
    12181 gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg tcgtccccac
    12241 aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat aggcctgcca
    12301 ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg aactgatact
    12361 cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt ccagagaggc
    12421 gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt tgataccgca
    12481 gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa tatcgatacg
    12541 cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg caggtcgctc
    12601 gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct gaattggcat
    12661 caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg cagtttgtgc
    12721 cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc gcaggccttt
    12781 gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac gttcttcgct
    12841 gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg aacgcagaac
    12901 gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt gcaactgact
    12961 gatcatactg gtcgctacat cataccctac ccgctgggaa tgaccgaact gtaaatccag
    13021 caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcgc ccgccccacc
    13081 cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc tgttggcgcg
    13141 acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg cggcatcagc
    13201 gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcgctgc gccccatcac
    13261 cgagataaag taatccccgt cacgggttaa aacgccttta aacatcggat cgtcattgag
    13321 caaagtgcgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg taccaccggg
    13381 gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga taattttatc
    13441 gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc tgataacacc
    13501 cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga cgcgcggtgc
    13561 gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggtggaggag gcggtggcgg
    13621 cacatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc gcttcaccgc
    13681 ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg catctttagg
    13741 cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg gcagtccctg
    13801 aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat ggctaaacgc
    13861 ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac cgttagaaac
    13921 ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca gcgtcgctac
    13981 caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac cggctaaacg
    14041 cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac ggccttgtgc
    14101 ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac cttgttgtgg
    14161 ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca taaactccag
    14221 ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat tgtcgagcgt
    14281 atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac ccagagcttg
    14341 taaaaaaccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca gatagattac
    14401 ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg cctgctcatc
    14461 agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg ttaacaatac
    14521 gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa cgcgccgcca
    14581 gtacgtcagc tgccgcaccg tgcgcgacac agcccgcaca ggcggcatca tacggcgaca
    14641 gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg ccgccgctcg
    14701 ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg gcgaccacgg
    14761 ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt ttggcgcaat
    14821 gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg gcctcgccag
    14881 gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc aggttcaatg
    14941 catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt gcttttttcc
    15001 cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat tccaggcttt
    15061 cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca gtcagcagcg
    15121 gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca cgcagcgccg
    15181 cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata atcaccagcc
    15241 gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt cagccagtga gaaagttgtt
    15301 ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccagctatcc agccccagtg
    15361 agtcaaaatg cagttgtcct gtaacatccc gcgcttttcc agtgagcaag cctggtttca
    15421 gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta gcgccggttt
    15481 cagccagcag gccggaaggg atatcaaccg ccacaatcgg cgcaggatgg gaattagcgt
    15541 ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta ccgagcagcg
    15601 catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg atctcgccac
    15661 ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt ttgtcgctct
    15721 cctgggccaa caacgtgacc tcaatgccga ccgctttggc cagtcgcgcg accacgtagc
    15781 catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg tcaggatacg
    15841 ccgaacgaca cacctggaat gcggcctcgc cagcgcgaag catcagctca tagagtgtga
    15901 gccccagcac atctgccgcc tcgcgttctc cgcggcggat atcgtcggcg taccagacgg
    15961 tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca gagcccctcg
    16021 atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc tttcagcagg
    16081 taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca tggctggaca
    16141 aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc gctcgccccc
    16201 atgagttatt accaggtacg ctgcgtgtaa tcagcgtgcg gatgaattac cttcctgcta
    16261 acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc cgttatgcgc
    16321 tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc gagatgattc
    16381 agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct attctcgagc
    16441 gcccgttagc tgaaaaagct gggctcggct ggacaggtaa gcactcactt atcctcaatc
    16501 gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg ctgcccgtgg
    16561 atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt tgcccgaccg
    16621 gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat ctcaccatcg
    16681 aacttgaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt atttacggtt
    16741 gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact acagaagacg
    16801 atttcagccc gcgtaagccg ctacacgcac aggaactcat tgagttattt gcctggagcg
    16861 aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt cacctgcgct
    16921 ggctgcgtaa tatcgccgtg gcattaggca atgcgccctg ggatgaaacg attctggcgg
    16981 cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg tgggcgattg
    17041 cgcagcaaat cgagagacga aatgcgtgca tagtcgaagt gcaattgccg aaaaaacagc
    17101 gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc cacagcttgt
    17161 gaataaaaat aaaaacgcat tgcaattcaa gacgcataaa atcgtcaagt gatcgactcg
    17221 acaaaacaga atcttgattt ttaacttatt tatcaatagg ttaaaaaaat accattcacc
    17281 gtgcgaagtt tctttgaaat taagattaca gcctgaggat gtggataact ctgtttacaa
    17341 gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg gatattttat
    17401 tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
    17461 tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca gcactttcaa
    17521 attttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag gtcgtgctct
    17581 accaactgag ctattcccgc ttaatcttcg tctttcgaac cgctactgcc ttggctgcct
    17641 tcactcacct tagtcacata cttttgtaag ttcccaagga ttcgttcagt tgccgccttg
    17701 tcgcaattcg aaatactgcg attgggtggt ctgtgctttg cagcactttt caaattttgg
    17761 agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact
    17821 gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta
    17881 cgggaggcgc attatacgag aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg
    17941 cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt caaaatcacg
    18001 ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc ttgataaaat
    18061 gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatt gcctgatgcg
    18121 tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc agctctttca
    18181 gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc atgtatttaa
    18241 acaggaaacg acggtcctgt ccgatgctgt taccgcaaat cggcgatttt cccgcaggca
    18301 cccactgttt taagaattcg agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg
    18361 ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag tcatccatca
    18421 gcgccagctg ttcatcggac tgatgtactg caatggtcgg cccttctgcc agaatattca
    18481 gattggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg ggatccagac
    18541 cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg ctattttcca
    18601 cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt ttgcccatca
    18661 ggggcgacca ggagtcagta cgattgagta aaaataaact ctccaaaggc cagcagcgcc
    18721 gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc gactacgacg
    18781 acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt atgcacgctg
    18841 atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc atccgttcgc
    18901 tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg
    18961 tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt ccggatttct
    19021 acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc tccgccattt
    19081 tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa accttgcaga
    19141 ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc atggcgttcg
    19201 tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg gtttccagcc
    19261 atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc agcatttttg
    19321 ccgggcagtc tggcgtcggc aaatccagcc tgctgaacgc gttgctgggg ctgcaaaaag
    19381 agatcctgac caacgatgtc tctgacaact cggggcttgg tcagcacacc acgaccgccg
    19441 ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt gtgcgtgagt
    19501 tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact
    19561 atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc tgcgctatcc
    19621 gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat caccgtattc
    19681 tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat gactgacaat
    19741 taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt aggccgaatc
    19801 aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac
    19861 agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca agcaagcggg
    19921 caggatggtt gacaaaactg gttatcgatc tgttcgttaa atactacaag gtcgacatga
    19981 aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc tttgtccgtc
    20041 cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtactggtc atgcctgccg
    20101 atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc
    20161 acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac ctgttccgca
    20221 acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccaccgcgta cacatgccgt
    20281 gcaacggtat tctgcgtgag atgatctacg tgccgggcga tctcttctcc gttaaccatc
    20341 tcacggctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt tgccttttcg
    20401 ataccgaatt tggcccaatg gcgcagattc tggtcggagc gacgattgtt ggcagcattg
    20461 agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag cgctggacct
    20521 ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa atgggtcgct
    20581 ttaaactcgg ttccaccgtt atcaacctgt ttgcaccggg taaagtgaat ctggttgagc
    20641 aactggaaag cctgtctgtc acgaaaattt gccagccgct ggcagtatct accgaaacct
    20701 ttgttacgcc agacgctgaa cctgccccgc ttcctgctga agagatcgaa gcagaacacg
    20761 acgccagccc attggttgac gacaaaaaag accaggtcta atccatcaaa ggaaacgctg
    20821 acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg gcgtacgccg
    20881 cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa gcggcgaaac
    20941 ccgcacagcc ggaagtcgta gaggcgcttc agtctgcctt aaatgcgctt gaggaacgaa
    21001 aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat ccgaaactct
    21061 ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacaa gccgcgcagc gtgtcgccgg
    21121 gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag ttgctggata
    21181 aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat tcgctgaatc
    21241 aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg
    21301 gaacgcttac cggcaatact ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact
    21361 ctgcacgtct taaggcgctc gttgatgaac tggagctggc gcagctgtct gccaataacc
    21421 gccaagaatt agcgcgctta cgctcagagc ttgcggaaaa agagagccag caactggatg
    21481 cgtatttgca ggccttacgt aatcagttga atagccaacg tcagcttgag gcggagcggg
    21541 cgctggaaag taccgaatta ctggcagaaa acagtgccga tttgccgaaa gatatcgtcg
    21601 cgcaattcaa aattaaccgc gaactatcgg cagcgctgaa tcaacaggcg cagcggatgg
    21661 atctcgttgc ctcgcaacag cgtcaggctg ccagccagac gttacaggtc cggcaggcgt
    21721 tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caatctgctc ggtgaagcgt
    21781 tgcgagcgca ggtggcacgg ctaccagaaa tgccaaaacc acaacagctt gataccgaaa
    21841 tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa cagccgctgc
    21901 tacggcaaat ccatcaggcc gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg
    21961 aagcacaact gcgcactcag cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc
    22021 tactattgga actgaccaag ctgaaagtct ccaacgggca actggaggat gcgctgaaag
    22081 aggtgaacga agcaacgcac cgctatctgt tctggacctc tgacgtgcgc ccgatgacca
    22141 tcgcctggcc gctggaaatc gcccaggatc tgcgtcgtct catttcgctg gacaccttca
    22201 gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacaatt ttgccgctgt
    22261 ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc acccgttttc
    22321 ttgaacgttc ggcggcgaaa gttggcaaag tgactcagga tcacttctgg ctgacgttgc
    22381 gcactctttt ctggtcgatt ctcgtcgcgt caccgttacc ggtgctgtgg atgacgctgg
    22441 gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc gatggtgtaa
    22501 cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg
    22561 gcttgtttat cgctcatttt ggctggccgc gcgaacgcgt atctcgtggg atgcgctact
    22621 acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg ttcgataacc
    22681 tcgacgaccg tgaattctcc ggttcgctgg gaaggctttg ctttatcctc atttgcggtg
    22741 cgctggcggt ggtcaccctc agcctgaaaa aggccgggat tccgctgtat ctcaacaaag
    22801 aaggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt ggcgcgccgt
    22861 tggttgccat tctggcgtcg gctgtgggtt atctggcaac ggcacaggcg ctgttagcga
    22921 ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac gttatccgcc
    22981 gctggatgct gatccagcgc cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag
    23041 agatgttagc gcaacgtgcg cgtggcgaag aggaagcgca tcatcacagt agcccggaag
    23101 gagcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg caatccttgc
    23161 ggctggtgcg ctcaattttg atgttgatcg ccctgctttc tgtcattgtg ctgtggtcag
    23221 aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc acctccacgg
    23281 tacagggcgt agaaagtctg gagccaatta ccctcggtgc ggtgctgatt gccattctgg
    23341 tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa ctggcgattt
    23401 tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc accaaatatc
    23461 tgctgatgct gattggcggg ctggtcggct tctcgatgat tggtattgag tggtcgaaat
    23521 tgcagtggct ggtcgccgcg ctcggtgttg gtctcggttt tggtttgcag gaaattttcg
    23581 ccaactttat ctctggtctg attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg
    23641 tgacaattcg cgatctcacc ggtagcgtga cgaaaattaa cacccgcgcc accaccatca
    23701 gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc gagcagttta
    23761 tcaactggtc gctctctgac tcggtcacgc gcgtagtgtt gacgattccg gcccctgctg
    23821 atgccaacag tgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg
    23881 tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacaaggg attcagattt
    23941 tcgagctgcg tatttatgcc gctgagatgg gtcaccgtat gccgctacgc catgagatcc
    24001 accagctgat tctggctggc ttccatgccc acggtatcga tatgccattc ccgcccttcc
    24061 agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg tctgcgggca
    24121 aaggtcgtca ggcgggaagt ttgtaatgca tgaagctgcc aaatgatgtc aacgtggctc
    24181 atttagcagc ttaacatcat cttcagttat caccggccct ggagagtatc atccatacgc
    24241 attacttcct gacaactcat cgccggagag acgatgagtt gcaggatttc atatgatcag
    24301 tcgagagcta actcaggttt attcgccatg cgactgcaat agttctggta aatcgccatc
    24361 gctaacagcg agaagaagac agggccgcca atcatccaca atgtgctgtc ccagtctccg
    24421 gcttcaacca caggctgaat aatggtgaag acgttcgcaa atgtcaccac cagtacgaca
    24481 accaccatcg caatcattgc cgacatacgc gttttgaaaa tcacaaatgg tctgtcgaga
    24541 tcctgacgtg ctttaaagaa tgggaaagcc agcgcgagga acaggtaagg aagcgtcata
    24601 gacacgttcg ccatcagcgt cagcttgtta aagaacgcgg atgcggtacc gccaccaaac
    24661 gaaaccagca ggatgaagac agtaaccaac ccgcactgca tccacatggc gatagacggc
    24721 atccccatcg cattcaggcg cgtcatcggt tccggccaca acgctttcgg cgtcccctgg
    24781 atgatggctt tcagcggtga atagcaaagc gtaaagaacg caccggtata agcgaggaac
    24841 atcgaaagtc cggtaatacg cgcaaaccat acgcccagcg acaatgacgc ttcaggtgac
    24901 aaatgcagtg cgttacccag cgtcaccccg aggctcttca tcagcacata ggtaatattg
    24961 ccaaggttaa cggaaccatt acttaatacc tgctgccagt ttgtgctgac gccccataaa
    25021 aatattgcca gcgaataacc gattgaaata acaatagcgg caaaaacaat acctttggca
    25081 aagttctttt ctggattttc cgttttatcg accagaccac caaccgcttc aattccgcca
    25141 taggcaaaaa tagcaaatac cacaaacgat agcattgcca gaccggactg ataacccggg
    25201 ttcggtgatg caaggaaatt aatatcctgc gcgaaatgcc caccatttaa taacaaaata
    25261 gtaatgctta ctaacagcaa tactaaattc agacacatta ctgcaatacc gcccaccgca
    25321 gtaatgcggg caattttatt aatccccttt gaagcaacga cggtgaccag aatcatccat
    25381 gctactgcca gcagaccaac cacctgcgta ggctccagtc cggcaatacg ccagtgctgg
    25441 gtcatgtcgc taccatagag gaatgttgag aacggtaccc aaactttcgc ggaggtactc
    25501 accatccaga tgatataaga ggaaaaccac ataaacgtac caatgaaggc aaaacgtggt
    25561 ccgacactat tattcatcca ggaatagata ccaccttctt ctttgcgata agcagctccc
    25621 atttcagcca tcattaa
//
LOCUS       DAZOZY010000040        25284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZOZY010000040 DAZOZY010000000
VERSION     DAZOZY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.040"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            17..268
                     /locus_tag="ACHURQ_001424"
                     /pseudo
     CDS             17..268
                     /locus_tag="ACHURQ_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="ACHURQ_001425"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="ACHURQ_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR0823245.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="ACHURQ_001426"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="ACHURQ_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR0823246.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="ACHURQ_001427"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="ACHURQ_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR0823247.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="ACHURQ_001428"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="ACHURQ_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR0823248.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="ACHURQ_001429"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="ACHURQ_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR0823249.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHURQ_001430"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHURQ_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR0823250.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="ACHURQ_001431"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="ACHURQ_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR0823251.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHURQ_001432"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHURQ_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR0823252.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="ACHURQ_001433"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="ACHURQ_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR0823253.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHURQ_001434"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHURQ_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR0823254.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHURQ_001435"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHURQ_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR0823255.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="ACHURQ_001436"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="ACHURQ_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR0823256.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="ACHURQ_001437"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="ACHURQ_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR0823257.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="ACHURQ_001438"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="ACHURQ_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR0823258.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHURQ_001439"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHURQ_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR0823259.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHURQ_001440"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHURQ_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR0823260.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="ACHURQ_001441"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="ACHURQ_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR0823261.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="ACHURQ_001442"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="ACHURQ_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR0823262.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHURQ_001443"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHURQ_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR0823263.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHURQ_001444"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHURQ_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR0823264.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="ACHURQ_001445"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="ACHURQ_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR0823265.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="ACHURQ_001446"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="ACHURQ_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR0823266.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHURQ_001447"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHURQ_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR0823267.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHURQ_001448"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHURQ_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR0823268.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHURQ_001449"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHURQ_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR0823269.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHURQ_001450"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHURQ_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR0823270.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHURQ_001451"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHURQ_001451"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR0823271.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHURQ_001452"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHURQ_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR0823272.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="ACHURQ_001453"
     CDS             14267..14635
                     /locus_tag="ACHURQ_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR0823273.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="ACHURQ_001454"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="ACHURQ_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR0823274.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="ACHURQ_001455"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="ACHURQ_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR0823275.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHURQ_001456"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHURQ_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR0823276.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHURQ_001457"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHURQ_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR0823277.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHURQ_001458"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHURQ_001458"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR0823278.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHURQ_001459"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHURQ_001459"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR0823279.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHURQ_001460"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHURQ_001460"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR0823280.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="ACHURQ_001461"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="ACHURQ_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR0823281.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /gene="smg"
                     /locus_tag="ACHURQ_001462"
     CDS             21312..21785
                     /gene="smg"
                     /locus_tag="ACHURQ_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR0823282.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="ACHURQ_001463"
     CDS             21814..22356
                     /locus_tag="ACHURQ_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR0823283.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHURQ_001464"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHURQ_001464"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR0823284.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="ACHURQ_001465"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="ACHURQ_001465"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR0823285.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="ACHURQ_001466"
     CDS             23753..24010
                     /locus_tag="ACHURQ_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR0823286.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="ACHURQ_001467"
     CDS             complement(23986..24540)
                     /locus_tag="ACHURQ_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR0823287.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25284
                     /locus_tag="ACHURQ_001468"
     rRNA            25013..>25284
                     /locus_tag="ACHURQ_001468"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6442 a         5992 c         6617 g         6233 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga tatcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataatgtcgc
    25201 aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga tgggattagc
    25261 ttgttggtgg ggtaacggct cacc
//
LOCUS       DAZOZY010000041        25106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZOZY010000041 DAZOZY010000000
VERSION     DAZOZY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.041"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            196..384
                     /gene="bcsR"
                     /locus_tag="ACHURQ_001469"
     CDS             196..384
                     /gene="bcsR"
                     /locus_tag="ACHURQ_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR0823288.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHURQ_001470"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHURQ_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR0823289.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="ACHURQ_001471"
     CDS             1145..1309
                     /locus_tag="ACHURQ_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR0823290.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHURQ_001472"
                     /pseudo
     CDS             1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHURQ_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHURQ_001473"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHURQ_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR0823291.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHURQ_001474"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHURQ_001474"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR0823292.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHURQ_001475"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHURQ_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHURQ_001476"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHURQ_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR0823293.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="ACHURQ_001477"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="ACHURQ_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR0823294.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="ACHURQ_001478"
     CDS             14459..15955
                     /locus_tag="ACHURQ_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0823295.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHURQ_001479"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHURQ_001479"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR0823296.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHURQ_001480"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHURQ_001480"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR0823297.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="ACHURQ_001481"
     CDS             18049..20109
                     /locus_tag="ACHURQ_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0823298.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="ACHURQ_001482"
     CDS             complement(20291..21613)
                     /locus_tag="ACHURQ_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0823299.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHURQ_001483"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHURQ_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR0823300.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="ACHURQ_001484"
     CDS             complement(23086..23985)
                     /locus_tag="ACHURQ_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0823301.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="ACHURQ_001485"
     CDS             24505..>25106
                     /locus_tag="ACHURQ_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR0823302.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5894 a         6366 c         6992 g         5854 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtggacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctgaacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggcg acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaacggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tggtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacag ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt accgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAZOZY010000042        24957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZOZY010000042 DAZOZY010000000
VERSION     DAZOZY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.042"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            67..294
                     /locus_tag="ACHURQ_001486"
     CDS             67..294
                     /locus_tag="ACHURQ_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823303.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(371..613)
                     /locus_tag="ACHURQ_001487"
     CDS             complement(371..613)
                     /locus_tag="ACHURQ_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR0823304.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            966..1886
                     /gene="lpxP"
                     /locus_tag="ACHURQ_001488"
     CDS             966..1886
                     /gene="lpxP"
                     /locus_tag="ACHURQ_001488"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR0823305.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2242..2313
                     /gene="ypdK"
                     /locus_tag="ACHURQ_001489"
     CDS             2242..2313
                     /gene="ypdK"
                     /locus_tag="ACHURQ_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR0823306.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2378..3616)
                     /gene="alaC"
                     /locus_tag="ACHURQ_001490"
     CDS             complement(2378..3616)
                     /gene="alaC"
                     /locus_tag="ACHURQ_001490"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR0823307.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3993..5690
                     /gene="ypdA"
                     /locus_tag="ACHURQ_001491"
     CDS             3993..5690
                     /gene="ypdA"
                     /locus_tag="ACHURQ_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR0823308.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5705..6439
                     /gene="ypdB"
                     /locus_tag="ACHURQ_001492"
     CDS             5705..6439
                     /gene="ypdB"
                     /locus_tag="ACHURQ_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR0823309.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6452..7309
                     /locus_tag="ACHURQ_001493"
     CDS             6452..7309
                     /locus_tag="ACHURQ_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0823310.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7312..9807)
                     /gene="ptsP"
                     /locus_tag="ACHURQ_001494"
                     /pseudo
     CDS             complement(7312..9807)
                     /gene="ptsP"
                     /locus_tag="ACHURQ_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(9832..10869)
                     /gene="ypdE"
                     /locus_tag="ACHURQ_001495"
     CDS             complement(9832..10869)
                     /gene="ypdE"
                     /locus_tag="ACHURQ_001495"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0823311.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10869..11954)
                     /gene="ypdF"
                     /locus_tag="ACHURQ_001496"
                     /pseudo
     CDS             complement(10869..11954)
                     /gene="ypdF"
                     /locus_tag="ACHURQ_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(11969..13216)
                     /locus_tag="ACHURQ_001497"
     CDS             complement(11969..13216)
                     /locus_tag="ACHURQ_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR0823312.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13238..13564)
                     /locus_tag="ACHURQ_001498"
     CDS             complement(13238..13564)
                     /locus_tag="ACHURQ_001498"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR0823313.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13784..14749)
                     /gene="glk"
                     /locus_tag="ACHURQ_001499"
     CDS             complement(13784..14749)
                     /gene="glk"
                     /locus_tag="ACHURQ_001499"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR0823314.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14953..16209
                     /locus_tag="ACHURQ_001500"
     CDS             14953..16209
                     /locus_tag="ACHURQ_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR0823315.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16324..16650
                     /gene="ypeC"
                     /locus_tag="ACHURQ_001501"
     CDS             16324..16650
                     /gene="ypeC"
                     /locus_tag="ACHURQ_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR0823316.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16791..18029)
                     /locus_tag="ACHURQ_001502"
     CDS             complement(16791..18029)
                     /locus_tag="ACHURQ_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR0823317.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18365..19567
                     /gene="nupC"
                     /locus_tag="ACHURQ_001503"
     CDS             18365..19567
                     /gene="nupC"
                     /locus_tag="ACHURQ_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR0823318.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19617..21805)
                     /locus_tag="ACHURQ_001504"
                     /pseudo
     CDS             complement(19617..21805)
                     /locus_tag="ACHURQ_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(22014..22089)
                     /locus_tag="ACHURQ_001505"
     tRNA            complement(22014..22089)
                     /locus_tag="ACHURQ_001505"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22054..22056),aa:Ala,seq:ggc)
     gene            complement(22129..22204)
                     /locus_tag="ACHURQ_001506"
     tRNA            complement(22129..22204)
                     /locus_tag="ACHURQ_001506"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22169..22171),aa:Ala,seq:ggc)
     gene            22425..22784
                     /locus_tag="ACHURQ_001507"
     CDS             22425..22784
                     /locus_tag="ACHURQ_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0823319.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22786..23178
                     /locus_tag="ACHURQ_001508"
     CDS             22786..23178
                     /locus_tag="ACHURQ_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR0823320.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23230..24645)
                     /gene="gltX"
                     /locus_tag="ACHURQ_001509"
     CDS             complement(23230..24645)
                     /gene="gltX"
                     /locus_tag="ACHURQ_001509"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR0823321.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(24758..>24957)
                     /locus_tag="ACHURQ_001510"
     CDS             complement(24758..>24957)
                     /locus_tag="ACHURQ_001510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823322.1"
                     /translation="RRPPPCKGGALPAELITPAVWSRIIGRVQNESTHFLKKLFVRRK
                     FKQDDRKTDRVAQFVNENNNA"
BASE COUNT         6219 a         6596 c         6132 g         6010 t
ORIGIN      
        1 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
       61 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
      121 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
      181 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
      241 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
      301 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
      361 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
      421 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
      481 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
      541 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
      601 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
      661 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
      721 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
      781 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
      841 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
      901 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
      961 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
     1021 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
     1081 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
     1141 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
     1201 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
     1261 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
     1321 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
     1381 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
     1441 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
     1501 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
     1561 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
     1621 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
     1681 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
     1741 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
     1801 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
     1861 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
     1921 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
     1981 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
     2041 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
     2101 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
     2161 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
     2221 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
     2281 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
     2341 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
     2401 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
     2461 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
     2521 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
     2581 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
     2641 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
     2701 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
     2761 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
     2821 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
     2881 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
     2941 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
     3001 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
     3061 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
     3121 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
     3181 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
     3241 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
     3301 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
     3361 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
     3421 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
     3481 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
     3541 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
     3601 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
     3661 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
     3721 agaacatttt tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
     3781 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
     3841 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
     3901 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
     3961 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
     4021 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
     4081 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
     4141 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
     4201 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
     4261 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
     4321 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
     4381 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga
     4441 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
     4501 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc
     4561 tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
     4621 tggatatcgc caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca
     4681 aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg
     4741 atcatgtgct ggcctatgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca
     4801 ttagcccgac tacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg
     4861 aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg gagaaagggg
     4921 tcgtgaccgg aacgctgaaa atttactact gccacgcgca tcagatcacc tcgtcattac
     4981 aggaaatggc ggtcggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg
     5041 agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
     5101 cccattttct gtttaacgct ctgaacgcta tttcatcgtc aatccgtctg aatccggata
     5161 ccgctcgcca gttgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
     5221 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
     5281 agcaggcccg ctttggtgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt
     5341 gctgcattcc cagcctgctg atccagccgt tggtggagaa cgccattgtc cacggtattc
     5401 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcgga aatcgggtac
     5461 gcattgcggt gcgagatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
     5521 cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat
     5581 tgtatggcga ggggctgcat atccgccgcc tggagccggg gacggaaatt gcgttttaca
     5641 ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga gccggagtga
     5701 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
     5761 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
     5821 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
     5881 ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
     5941 catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
     6001 tctcaaaccg tatcaggagt cacgtattac cgggatgctg caaaaactgg aagcggcctg
     6061 gcaacaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga atgacaccat
     6121 taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga
     6181 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
     6241 cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt
     6301 ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca cctacattct
     6361 gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga aagaatttcg
     6421 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
     6481 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
     6541 ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg attttccccg
     6601 tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc agcagcgaat
     6661 ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga tcaacaacaa
     6721 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
     6781 tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca gcctgcaacg
     6841 cggtgaaggt gaagcgatgc ttaccgccct cacccatctt agccgttcgc cgctggagca
     6901 gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg tggtgaatat
     6961 gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt gtaactgggt
     7021 tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac
     7081 gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta tcgagtatgt
     7141 gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc tgtcaattca
     7201 tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg ttttccggcg
     7261 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
     7321 taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa acaacgactg
     7381 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
     7441 cccttcgtta gcacccagcg tcagcatgat caccagttca acttcgccca tttctgactg
     7501 ccagtcaacc ggtttcgcca gccgggcaat gctgatactg gaatgacgga tccactgaga
     7561 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct cttcccgctg
     7621 ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc cgaggttgcc
     7681 gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa agatattctc
     7741 cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat
     7801 ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
     7861 cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac tcagctcatc
     7921 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
     7981 gcctacccat ttgccccgct gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa
     8041 cgatggcgta atcgggttat atagcggcga tacgcgcggg ttattacgat cgaccgcata
     8101 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cggagaagtg
     8161 gtcgatgatg tagcacaccg acggcacttc caccatgatc ccaagcgtaa tcgtctctgc
     8221 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ctttcaccca
     8281 taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc cgaaactggc
     8341 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaatacg
     8401 taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat aaggaatgct
     8461 tttatcgccg ccgatgtcca tcgtgcggaa gataatcggc ttgtcgcccg ccgccagtag
     8521 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcgc gatccatata
     8581 gagcatttcg gtacggaaca gcccgacacc ttccgcgccg ttggcaaacg cgcctggcgc
     8641 ttccagagcg gtgccgatat tcgccgcaat atcaatacgc ttgttatcac gggaataggc
     8701 cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg tctgcgcgac
     8761 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc
     8821 aagcactgca ggttgcccgg cataacgggc aatcgcatcc agcggcaagc cactcagtac
     8881 cgggatcgcc gaggcacggg ccagaatcag tgtatgcgag gtgcgcccgg ttttctccag
     8941 aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg ttaaatcttc
     9001 agccaccaga atggtcggtt tttccagcac cagattgttg cgcggcttca gttccggcta
     9061 ggtgatatgc agcaactgtt cgctgatatc gcgaatgtcg ctgacccgct cgcgcagata
     9121 atcgctggca gaagcagata gtttggcgca aacctgctcc atattgctga tgatcgccgc
     9181 ccccagtccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa attcatcatc
     9241 ctgaatcagc gacaaatgag cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
     9301 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
     9361 cgcactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca gggtcagcgt
     9421 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt taaggcggct
     9481 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtcggctgaa cgctgtcgct
     9541 gtcgataaag cgcaccagga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
     9601 gccgctaatg ttcaggctgc aactgtcatt aaataaggtg ccagttccaa tcaacgccag
     9661 cgagctttta gcatcggcct ttgcgttctg gcgatggttg ataaaggtga tttcgctttg
     9721 ccactggctg cactgttctt taagttccca cgccggacga gcatgtagac cgttaggcaa
     9781 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
     9841 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagttg
     9901 ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc gcgcaatgtc cgtggcgggt
     9961 ggcaggcccc attaccactg tgggtacgcc agtatcggtt aagtgcaccg ctccaccgtc
    10021 cgtgccgccg ttactgaaca tatccgcctg caacggaacg ccaatttctg ccgacacggt
    10081 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
    10141 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    10201 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtagcag tttgcccgcc
    10261 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gcagttcggc
    10321 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcggt catcaaaggc
    10381 tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc
    10441 gcctggacga attcccgcct gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg
    10501 catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    10561 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca cgttgccaac
    10621 cggcagcaca tcaattgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    10681 catatgcgca cagatcatca ccttcggacc tgtcgattca ttgaggcgga tcagcaccga
    10741 tcccagacca tcaaatcgta cttctttttg caggcgatcc gcttcttcca gtaggatctg
    10801 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
    10861 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    10921 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctggcaaa
    10981 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt atcccgcggt
    11041 gaaaagcgtg gatcttcgtg tacttcaata ccgatagcat gaccggtgtt gtgaccaaaa
    11101 tagtcgccaa atcctgcctc ggtaatcacc cggcgcgcgg cgtcgtcaac ctgttagcag
    11161 cgtacgccag ggcgaatcgc ggagattgcc gccagctgtg cttgcaggac aatctgatag
    11221 acgttaaaca gcgggtgaga ttcggcaatt accccttcgc cattcaccag caaggtgcgc
    11281 gtcatatcag agcagtaccc ctgatagagc gcaccaaaat caagagtgac aaactcgcct
    11341 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccacgcca gccgctggcg
    11401 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    11461 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    11521 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    11581 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    11641 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    11701 atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag
    11761 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    11821 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    11881 tgccgtgagg agagaagcac tgcatccagt tgttgcgcct taagccagtc gcgcagcgaa
    11941 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttacctttac
    12001 ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc cccagcgcga
    12061 tccccgccat atagacgccc aggttagtga ccagcggcca tgcccaaata gcggattccg
    12121 ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaatc gcgccgacca
    12181 tatacgacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    12241 tccccataaa ggcaaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    12301 ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag acccagacct atcggcggaa
    12361 taacgatagc gatagaacgt gcagtgaccg gtaatacgtg gtcagtggta aaactgaagg
    12421 caacaaaacc tgcggctttg ttgatcgggc caccaaggtc gattgcggtc gccgcggcga
    12481 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcacg gtacggatac
    12541 cgccgttgat ccagccgcca aagggggtaa tgacgtagta catcgccagc atgacaaaaa
    12601 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    12661 ggattttctg gttcatccac ttcacaaggt aacccgcgac gatagaaata atcagcgcac
    12721 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    12781 tcagcagttg cgtcggctgg gtagacatca agccgccgat aaaaccagcc gggaacgcca
    12841 gtttgccacc gatagagttc gccacaaaag cagcaaacat cggaatggca aaaccaaaca
    12901 acacgccgcc gaaggactgc gacagccagg caaatttcag taaggagagg tcgaaaccgg
    12961 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    13021 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    13081 tgcgtgaaat ccctaacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    13141 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    13201 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    13261 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    13321 cataaacgtc gcgtgattcg aaacgctcat tatcttccgg ggtaactgcc acggagtgga
    13381 tgatgatggt tgcttcggcg atatcctgcg ccgtcaggcg attctggata ccgtccgcgc
    13441 cctgggtttc aattttcact tcataacctg cttctaccgc cgcttcttcc agcgcctgag
    13501 cggccataaa ggtgtgagcc aggcccattg ggcaggcaca taaggcaatt agtttcttac
    13561 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    13621 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    13681 tgacgcaggt cggccttgtg agggggacac aaagaaaaat tgaattaact gtatgattta
    13741 aaagattatc gggagagtta cctcccgata taacagaaag gatttacaga atgtgcccta
    13801 aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat
    13861 acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga
    13921 aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgccagca atataaaccc
    13981 caccaaaggt tcccagcgtt aatgccagat tgccgccaaa acggcccata atgacgcaaa
    14041 acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat
    14101 cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat
    14161 tcaccagccc aggaccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca
    14221 atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt
    14281 cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg
    14341 ttccggcacc gtaaaccgca ataggtttac cttcgaccgg ttctgcgcca ccaaactgaa
    14401 tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa
    14461 tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat
    14521 ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt
    14581 tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat
    14641 caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa
    14701 gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc
    14761 tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac
    14821 gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact ttatcgtttc
    14881 agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat
    14941 aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc
    15001 gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta
    15061 ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct
    15121 atctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc
    15181 agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accagttccg
    15241 ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc
    15301 ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct
    15361 cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc
    15421 ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc
    15481 agtagtgaag ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt
    15541 gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga
    15601 cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca
    15661 gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat
    15721 ccggtgctcg tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga
    15781 ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc
    15841 agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc
    15901 gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg
    15961 ttgatgctgc atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt
    16021 ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc
    16081 gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg
    16141 ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat
    16201 aatgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct
    16261 ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata
    16321 acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca
    16381 aatgcaggtg aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat
    16441 tacggtaatt actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat
    16501 gaatggcgca aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag
    16561 cgtaaagcat atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga
    16621 aaaggccgcg gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga
    16681 tgcgacgcta gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata
    16741 aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa ctacaatccc
    16801 agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag
    16861 cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg
    16921 aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata
    16981 accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg
    17041 actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc
    17101 cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag
    17161 accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg
    17221 gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga
    17281 ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt
    17341 tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc
    17401 ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa
    17461 ctcgggatca ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg
    17521 acaatgtaag ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagtggtttt
    17581 tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc
    17641 tgcaacaacg agacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc
    17701 aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc
    17761 ggatagtgat cacgaatctg ctccgccaga ttttttccgg tggcaatccc tagtttggca
    17821 gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga
    17881 tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca
    17941 atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg
    18001 ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg
    18061 tttcatgcta tgataaacga gaatgattat caaattcatt taaatggatt gtggtgattt
    18121 ctctgataga ccaggattat gactacgaaa agattgatga cttgaatgtt atggttatgt
    18181 ttaatgttag cacatttaca taactttcag cttccataca caacatagca gaaatgtatg
    18241 acagatcact atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt
    18301 tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc
    18361 aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact
    18421 gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat
    18481 cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg
    18541 cttctccgaa atgttcgaaa aactgctcag atttgccaac gaagggacta acttcgtctt
    18601 tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt
    18661 ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg
    18721 cgcaattggt ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc
    18781 cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag atatcctcgg
    18841 caaaatctcc cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat
    18901 gtccatcgtt ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt
    18961 actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc
    19021 cagtgaagaa aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct
    19081 gggtgaatac attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg
    19141 ctttatcgcc ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg
    19201 ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat
    19261 gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc
    19321 caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc
    19381 tgaaggcatc atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat
    19441 cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct
    19501 gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt
    19561 gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa
    19621 tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta
    19681 tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc
    19741 gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata
    19801 tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg
    19861 tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc
    19921 tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca
    19981 agaatgaccg cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgcccggca
    20041 atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg
    20101 tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc
    20161 gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg
    20221 tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc
    20281 tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc
    20341 acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga
    20401 tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt
    20461 tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg
    20521 ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt
    20581 tccggcccgc tcagcactaa cagcagttca ctacccggca attgatataa cttttcgttt
    20641 tcctgcatca acggcagcag cgtacggtaa attgatcgga tacagtgaac gcgcatcatt
    20701 aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaactcttgc
    20761 ccgcctcttg ctccggcgct tgttccaacg cacggaagtt gggcaggagc gttaacggat
    20821 cggtcagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    20881 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc aagaataaat gccagcgaat
    20941 attcggtttc caccccttgc aaaaagttct ggttgtaatt cagaaggcaa agcgttgaaa
    21001 cagcccaggt gagatttaaa aacggatagc gaagcttacc gaccccgagg gtaaagatga
    21061 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    21121 gaagtagaag cacgcttaac gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc
    21181 ccaacgacgg cgcgatatcc ctgcgccaca atatctgcgc aaagtgggga cttacaatca
    21241 tgcgagtgag atagtagaaa agcatgttgt aaataagcac agcggtgaaa aggcttagca
    21301 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    21361 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    21421 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    21481 ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg ctaccgagca
    21541 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    21601 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    21661 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    21721 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    21781 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    21841 acacaaataa taagtttatt cgcatcgcga agcaaacagg gggccagcct gttgccgatt
    21901 tttcatccaa tgatgctttc accaggcggc actaacccgg acgtagactg tattacaaaa
    21961 gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa atttggtgga
    22021 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    22081 tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa
    22141 gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag
    22201 ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt
    22261 gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt
    22321 ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta
    22381 taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc aaggagagga
    22441 tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt aataaatggg
    22501 tacgcaagga gggctggaca acgtcaccaa aacctggcgt ccagggtggc aaagcccgac
    22561 tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc
    22621 agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg gtgacactgg
    22681 cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga
    22741 ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc
    22801 aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac
    22861 cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt
    22921 gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc
    22981 cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc tcctggagca
    23041 cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga cattgagcag
    23101 ggaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat
    23161 attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt ttatcttttt
    23221 acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt tgttgatacg
    23281 ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg gagactgccc
    23341 cgccccggtt acggcgacac gcagcggcat accaacttta cccataccca cttccagctc
    23401 atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcggc
    23461 cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat gttttttcgc
    23521 cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc
    23581 tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct gcgggccgtt
    23641 acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg
    23701 cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact
    23761 ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac gagtgaagat
    23821 ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg cttctggcaa
    23881 ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg acagtttttt
    23941 accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc
    24001 cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg
    24061 ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc
    24121 ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg gaccacggat
    24181 ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa cacacggttc
    24241 atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc
    24301 tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata
    24361 agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt
    24421 ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat ccataatggc
    24481 ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc
    24541 accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg
    24601 cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt
    24661 ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac
    24721 tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg ttttcgttga
    24781 caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga cgaacgaaca
    24841 atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcgg ctccacacag
    24901 cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggt
//
LOCUS       DAZOZY010000043        24907 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZOZY010000043 DAZOZY010000000
VERSION     DAZOZY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24907
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.043"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..702)
                     /locus_tag="ACHURQ_001511"
     CDS             complement(<1..702)
                     /locus_tag="ACHURQ_001511"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0823323.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPATLALSVNDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRET"
     gene            complement(683..2284)
                     /locus_tag="ACHURQ_001512"
     CDS             complement(683..2284)
                     /locus_tag="ACHURQ_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0823324.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRDRLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            complement(2281..2487)
                     /locus_tag="ACHURQ_001513"
     CDS             complement(2281..2487)
                     /locus_tag="ACHURQ_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR0823325.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(2484..4409)
                     /locus_tag="ACHURQ_001514"
     CDS             complement(2484..4409)
                     /locus_tag="ACHURQ_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0823326.1"
                     /translation="MNISNSQVDRLRHFVRAGLRALFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPASVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDSAAT
                     NKKTLADYARALSGEDE"
     gene            complement(4384..4929)
                     /locus_tag="ACHURQ_001515"
     CDS             complement(4384..4929)
                     /locus_tag="ACHURQ_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR0823327.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRLASEADLHPGTLEFERHRLTRAQATAQELKNA
                     KESAEVVETAFCTFVLSRIAREISSILDGIPLSVQRRFPELDNRHIDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            complement(5069..5293)
                     /locus_tag="ACHURQ_001516"
     CDS             complement(5069..5293)
                     /locus_tag="ACHURQ_001516"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823328.1"
                     /translation="MIRGFVPHARQLCAGVEETGHVFRSVRPWSHDAGLSDIEFIANK
                     PERLRVLSGDPAGYGAEGARVFAIYENFPV"
     gene            complement(5527..5976)
                     /locus_tag="ACHURQ_001517"
     CDS             complement(5527..5976)
                     /locus_tag="ACHURQ_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823329.1"
                     /translation="MQEEAKKILVDLLNNASDGIDSAIAFSQAQIPDVVHQLLVWNIV
                     DSLIKTLIAISTIPLVIWFMKKQCKKVEIGKFDNDGRSCDNGQPKYKPTMIWESDGRL
                     SAFVLPLVAVFILWLSFIISIVANMTWLKIWLAPKLYLIEYAASLIK"
     gene            complement(6102..6962)
                     /locus_tag="ACHURQ_001518"
     CDS             complement(6102..6962)
                     /locus_tag="ACHURQ_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823330.1"
                     /translation="MKELQKINSIMERLIALELKLADCGKLCAEKQNELYREFVTIVS
                     ELMPIVNNCLMTSRFFNVDATTSRKFTVKIQERVSGSVTREMAEQFVAMSNKTKRKKA
                     LLVTVNQLSGRVDFQVRQGGRVLVNDAFVGRCFSPYSMKYDVDADDQDISISWKSETA
                     GIVLTAELLSDDKNDGQHHLDEKNHISEICMKPRSGMTLLSRTLAAGFSATEKRVLLV
                     VPEDADIRPLNGCDVISAKTLHTRIMPVPDVVIIDDIEKCKSVSSTATIKRILRCSTS
                     AVVFQKQLHN"
     gene            complement(7243..7554)
                     /locus_tag="ACHURQ_001519"
     CDS             complement(7243..7554)
                     /locus_tag="ACHURQ_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFR0823331.1"
                     /translation="MKIYIAGPMTGYENFNRDAFNKEADRLSRHGHSVLNPATLPNGL
                     TQREYMDICFAMLRCADAILMLPGWKASAGATAEYHYAYKMEMPVFTTLNYPPACSSA
                     V"
     gene            complement(7596..8063)
                     /locus_tag="ACHURQ_001520"
     CDS             complement(7596..8063)
                     /locus_tag="ACHURQ_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0823332.1"
                     /translation="MSRVTAIISALVIGIIVCLSWAVNHYRDNAIAYKEQRDKNAREL
                     KQATATITDMQQRQRAADALDAKYTKELANAKAENDALRRKLDNGGRVLVKGKCPVPA
                     SAKTSSASGMGNDATVELSSVAGRNVLGIRDGIIRDQTALRTLQEYINTQCLN"
     gene            complement(8060..8557)
                     /gene="rrrD"
                     /locus_tag="ACHURQ_001521"
     CDS             complement(8060..8557)
                     /gene="rrrD"
                     /locus_tag="ACHURQ_001521"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR0823333.1"
                     /translation="MQSSLRKAVTAAIGGGAIAIASMLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCYGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(8557..8829)
                     /locus_tag="ACHURQ_001522"
     CDS             complement(8557..8829)
                     /locus_tag="ACHURQ_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823334.1"
                     /translation="MKMDERYSSASYGSAGLAAFFASLSLQDWGFIIGVAFSIILGVL
                     TYRLNKREQMKRTKILQDILDKTNTNNLSATAMVIGELGKRAPKEI"
     gene            complement(8925..10001)
                     /locus_tag="ACHURQ_001523"
     CDS             complement(8925..10001)
                     /locus_tag="ACHURQ_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR0823335.1"
                     /translation="MFKTTNIHGAQLVCADSLQFIKTIPDNSVNLIATDPPYFGVKAN
                     AWDNQWDSDADFLGWIDEFLAEFWRILAPNGSLYMFTGSRRASKIELLTRDRFNVLNH
                     IIWAKPSGMWRGCHKESLRAYFPATERILFAEHYGASGYAKGQSGYASKCADLRKNIF
                     SPLIDAFSLARRQLDISAADINSATGKQMCSHWFSYSQWRLPSLIDFNKLCELFRRKA
                     DSLGVPCPYPFNVDYSEHEKRYSDLKLHYEEVKKQYDDLKAQYESLRRPFHVTADVPY
                     TDVWEFPPVQYYPGKHPCEKPAAMMEHIIKSSSRPGDIVADFFMGSGSTIKAALKLGR
                     QAIGIEIEGERYLQTVDEVKKLFE"
     gene            complement(10269..10496)
                     /locus_tag="ACHURQ_001524"
     CDS             complement(10269..10496)
                     /locus_tag="ACHURQ_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823336.1"
                     /translation="MNINEIRGNMTEAALSVECVMRGYPRISLKELSEACFLSQAAVE
                     FIIEQMICFGVAKRSGSGRYSLTDEYKQATF"
     gene            complement(10510..10731)
                     /locus_tag="ACHURQ_001525"
     CDS             complement(10510..10731)
                     /locus_tag="ACHURQ_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823337.1"
                     /translation="MNYQGNEKMRGDVAEIANELYELWQKVERFEKEYGFNSTNLTDR
                     LAGRLIGTMGPKLAELNRFMADVDFQFQD"
     gene            10895..11206
                     /locus_tag="ACHURQ_001526"
     CDS             10895..11206
                     /locus_tag="ACHURQ_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0823338.1"
                     /translation="MFNVITHPAALDELLELPDDLRGRMTRLIERLEKEGNKLKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPVKAIEIARTRLKEM
                     N"
     gene            11206..11493
                     /locus_tag="ACHURQ_001527"
     CDS             11206..11493
                     /locus_tag="ACHURQ_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0823339.1"
                     /translation="MKAKGIPFNEVKEKLLNTPEAIKGYEEADKELEMVEMLYEMRER
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLSKYAQACGATININAVY"
     gene            complement(11537..11740)
                     /locus_tag="ACHURQ_001528"
     CDS             complement(11537..11740)
                     /locus_tag="ACHURQ_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823340.1"
                     /translation="MTEASVSTLEELGSNLKDIDAVLDLVSVALASPEASLHLGEISR
                     LVSMSREIAQNCQQAIAVECLHH"
     gene            complement(11997..12323)
                     /locus_tag="ACHURQ_001529"
     CDS             complement(11997..12323)
                     /locus_tag="ACHURQ_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823341.1"
                     /translation="MALTAAERQRRRRERLKKEGTSRRDWILEPDELRMLGEICVLRR
                     PGRVPYSENEVIGLLIRKNYKELQKQLSTTCPRCGQKMPVSECIFDGEGSCHLTTTRL
                     KLVLKV"
     gene            complement(12339..13013)
                     /locus_tag="ACHURQ_001530"
     CDS             complement(12339..13013)
                     /locus_tag="ACHURQ_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0823342.1"
                     /translation="MKFSKFSELVNRILLNRILSNNHSDRRDMDVTIVVHSPGSIGST
                     PSVEVQSIHAGFDWDSGKVLIFPAQPLTTLTPEQITDITDSVRKGQSWHAYQEYKKHK
                     EQLEKLSIELESAKNHIAELEAKPVSQTYKLTFEQWLEQQHDKIDVDCGCVSTEMLMH
                     WIRVAYEAGNSPVTPDGHIQFSVSLPAACGGDKYFIDGVFQPLRYERDCEKAVVAAGG
                     VVNWVK"
     gene            complement(13010..13300)
                     /locus_tag="ACHURQ_001531"
     CDS             complement(13010..13300)
                     /locus_tag="ACHURQ_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFR0823343.1"
                     /translation="MNIDTTITIDTALNTGLALLGWFYIMFSAGKWAASVFLKQWGKR
                     RKQEKRQKALEAFYDAFELSRIEPGTTAKIATKGDLMIVMFRQERAEKGESA"
     gene            complement(13300..13719)
                     /locus_tag="ACHURQ_001532"
     CDS             complement(13300..13719)
                     /locus_tag="ACHURQ_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0823344.1"
                     /translation="MAKAFTPEQREELNKQIVELVRLNGRGTVRQLADETGISRCAVS
                     RLSRELAASGDLYISGSGIFLSAQARKDWQNARKKLSRVKPKKSVVVDPDLIWSLPDG
                     EIRRYDRRLNMICHDCRKSEVMQRVLAFYQGNFQEVA"
     gene            complement(13719..14276)
                     /locus_tag="ACHURQ_001533"
     CDS             complement(13719..14276)
                     /locus_tag="ACHURQ_001533"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFR0823345.1"
                     /translation="MIDLETMGTNTCAPIVSIGAVFFSPESEELGPTFYVPVNLRSSM
                     LNGGIADGDTILWWLKQSKEARAAICTNDALDIKDALFELSHFITCHACNLKKLKVWG
                     NGATFDNVILRGAYERVGLACPWEYFNDQDVRTIVNLGQFIGFNPKKDMPFDGERHNA
                     LADAIHQAKYVSAIFSRLVKGQGES"
     gene            complement(14342..14515)
                     /locus_tag="ACHURQ_001534"
     CDS             complement(14342..14515)
                     /locus_tag="ACHURQ_001534"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823346.1"
                     /translation="MTHDEAERLSDTYRRRGKKVLVVRSDFLGDGYCVYVHLPESERT
                     PKPSRTYQQKIWV"
     gene            complement(14779..15489)
                     /locus_tag="ACHURQ_001535"
     CDS             complement(14779..15489)
                     /locus_tag="ACHURQ_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR0823347.1"
                     /translation="MELEYIRSCVSTALADVHYRQRGILEVQLEQMRLGRSGRFNNKP
                     VRSISVGDDNSYEVSVPAEPVRFHQGKTFKQSSMLLTDIDFQSASWRRAIGQLNNEES
                     AWLYYCYGCKPDYNNDVIVCQWLWLDFLVAHSGGGFKKMKASTKKAMRKLIYYAAQQV
                     KSELTCAEAVDEREQDRHLSFLLNISIDSWRQDYKERWLLIKSRCLSLNRTALLNAAE
                     KRSEIIKRHRAGSAILPL"
     gene            complement(15479..15700)
                     /locus_tag="ACHURQ_001536"
     CDS             complement(15479..15700)
                     /locus_tag="ACHURQ_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0823348.1"
                     /translation="MKPEELIRHFGSVEKAAAGVGVTPGAVYQWLTAGEIPSLRQSDI
                     EVRTAYKLKSDFTTRRVKQSIKESRKRGA"
     gene            15792..16388
                     /locus_tag="ACHURQ_001537"
     CDS             15792..16388
                     /locus_tag="ACHURQ_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0823349.1"
                     /translation="MKTRGERLKARRLEMKLTLREVAEAVDISIAGVQNLERGDVMPS
                     LEIGIALAKCLRKPVHWVLYGTEYDPDRIPVIGTTESGPDEEWKPGQVPNTERFLPFI
                     SQRNTVYALTISNLTQGNYQPGDFLLVDSALELVPGEDVLVRDTAGNITIQRLARFDD
                     ENYYLDGTNSQRAIFRKSDLEFVHQIVGTIKSFMVEGR"
     gene            16634..20629
                     /locus_tag="ACHURQ_001538"
     CDS             16634..20629
                     /locus_tag="ACHURQ_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein, CyRepA1 family"
                     /protein_id="HFR0823350.1"
                     /translation="MTLNDFYADRFGSDPFSLIEAARDELTELAQMAGINWPACSDNI
                     QLNPRGGVERYSSYNKTSPEALEKSLKGRVEIYSRLEHSKGIDYPFINFVHKGSDAGS
                     WSGFSFLFSEYRREQQRNNATVVAQPEEERERIERQAEARRRREEQKRINDLKNNQMD
                     HERLLGWLAFHSAWEHAQAEDGSWPYAIKKGIRDVFSSCDVRRVTSHDSAKWSRGPTT
                     YMAIPLAHLDGRYDGRIVGWQRIDKQGGKYQTSAVTSGDFVGACFVIGELKGAQNIAV
                     TEGFATGASIWLATKKDKRFDAVVVAVSANNMVHVVEQLVNMYPAAKITCALDNDRKS
                     ATEGKGNTGLRTGYEILSKFNDVRCVYPTFEDDPQMECSDFNDLHKLRGLRETARQLF
                     ATANRLKTGTDLLSLTLNRLKTIKRDNRRTFAKELLRAVDIGMLTCPVPNSPSDLFNM
                     FCIVLREMGLENIYRATVKDHIARRLNKKCRIAQAPRSFSERITDPNKRPQHITYKRF
                     ETSVMTDDVLKYVQSLQGIVIVRAGMGSGKSTGLLRPLMHSSERGISVAHRVSLIGGL
                     WEMMTEGKGVKADILHYQDPGYQEMAPYASKLTICINSIVKNCWQPLMRQHDYFGFDE
                     ATQGLRAVLSGRAMENPVAVFNTLIDALARTEQHPIMVDADANDLLVDLAELAMKRRE
                     ELGLPAWLQIHVIELPVDVRNRETGEPIRVFYTEKDRIMTEVVKAVSDGEKIMLATDS
                     STFAEDVTATLRMHFPGKKFLCVNQKNKPEPEVEAFTNQPKKMVKKYDGLIYSPSISS
                     GVSIEQKHFDRHFGMFCGEVVPSDAVQMLRRDRTAKEFIIGFDKARAKRETDPQKIER
                     AFAQALLATAGMNGELTDVVFDGDRISMGVANTDFTRMKIKAAAIEATARNDYASNMI
                     CIMHSDGYKVSPLATDYAANDIGKDLRKEARDIVWEQTLDLHLNTETPQESEREELLK
                     KRALTLEEQAKLVRWDIENELKLPVEEGTLKFYFDGARDKVRRYETMLLDEVTARRYD
                     REEAAINFTYAYRQAGQWQYFVVTAMTREQADDVFKTKFPGIIDHKVKATPTVEVGAR
                     GFYALKSATLRQYFIDCGINPETMTGEATQERLKFARDNLMTAERRDLLNNVLHIGGF
                     MTPKGKPKVPEALFKNICDSLGLKTGKRRGRDGDKRPTLRFVEPESAAFMLDIIVQRQ
                     KDGLTLKTRKAEKTAIEMDRDLDLNIYMDDKTRSTNEQVQNAPHSVISEALAVLPVAV
                     PESWAMSALSSEELATMSSWSSASIAMTFASLYLTEFMDHLSSNELRRLREYITGNSS
                     CSYEEQGAFYG"
     gene            20818..21144
                     /locus_tag="ACHURQ_001539"
     CDS             20818..21144
                     /locus_tag="ACHURQ_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823351.1"
                     /translation="MSTTAFLDKLDYRQLQFCRDECERRIRAIQEEEKKVAWAVTDRG
                     LIFGWFRTEDYLKAVELLARKAAERWERADKENPETKYELNLYIEGNRLPLSEYNALF
                     ADGEWG"
     gene            21147..21542
                     /locus_tag="ACHURQ_001540"
     CDS             21147..21542
                     /locus_tag="ACHURQ_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823352.1"
                     /translation="MAVQSYKYESPLDKGYIRIKLSRYQHKAIFKVRKIRILDAYAYY
                     YNGENVVVEHFLARWFVALIFVPLLLIGTFVDGFPSTWREIKKGLFPRKYGRFSQDSW
                     RVIPGKHSVDEQPIVDFWLNRMADAKKVD"
     gene            21570..21857
                     /locus_tag="ACHURQ_001541"
     CDS             21570..21857
                     /locus_tag="ACHURQ_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823353.1"
                     /translation="MKSFIFVLCFAFSAAVYAQEDSISVINSSADLACKNNPDAASKE
                     SCKELLLSTAAFASENAKFYSAFCRGYVKDEDKEKCDDAGKLVRYFQSLNQ"
     gene            22234..22398
                     /locus_tag="ACHURQ_001542"
     CDS             22234..22398
                     /locus_tag="ACHURQ_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR0823354.1"
                     /translation="MFKFKFAAIVRTDKKSHIHHLSTIASSEREARRQFASRFVLVLS
                     ARIPVREVAA"
     gene            22395..22628
                     /locus_tag="ACHURQ_001543"
     CDS             22395..22628
                     /locus_tag="ACHURQ_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823355.1"
                     /translation="MNQVKMNTVGLLESLDERLAQVYALVSSAHRTISSCEASIYLSE
                     AGKLLQIACELTHEAQGYSASLSSQLLHDKEAE"
     gene            22625..22789
                     /locus_tag="ACHURQ_001544"
     CDS             22625..22789
                     /locus_tag="ACHURQ_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFR0823356.1"
                     /translation="MSALAVFSFQEEHQVRVVMINGEPWFVASDVCMAAGIDSTAIRK
                     LDEDEKGQAR"
     gene            complement(22835..24730)
                     /locus_tag="ACHURQ_001545"
     CDS             complement(22835..24730)
                     /locus_tag="ACHURQ_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="telomere resolvase"
                     /protein_id="HFR0823357.1"
                     /translation="MSKVKIGELINSLVSDVESIDNSDRPQGEKTKKIKAVALKFKNA
                     LFNDKRKFRGKGLEKRITANTFNAYMSRARKRFDDKLHHSFEKNIHRLSEKYPLYNEE
                     LLSWLSLPAADIRQRMSALQAKLKLILPLSEDLSNIRIGDNKSHNKLLKLSNKYKEWQ
                     FAISDLTSDNWKDKRDYLYKLLQQGSALLEELNQLKVNHEILYHLQLSPAERASIQQR
                     WADVLHNKKRNVVVIDYPKYMQAIYDIINSPATLNNLNTRSGMAPLAFALAALSGRRM
                     IEIMYQGTFTVSGKYTVDFLGQAKKRTSDDITRKIYTLCEAKTFVYLINVLRSCPAAS
                     DFDDVVKGYGDNDTRSENGRINAILAKAFNPWVKSFFKDDRRVYKDSRAIYARIAYEM
                     FFRVDPRWKNVDEDVFFMEILGHDDENTQLHYKQFKLANFSKTWRPNTGNENTRLEAL
                     QQLDDEMPGFARGDAGVRLHNTVKQLVEHDPSIKITNSTLRAFKFSPTMISRYLEFAA
                     DALGQFVGENGQWQLKIDSPEIVMPDEEELEAIPEQTDELSEDDDLDDDEIEIEEEEE
                     EESVEEQQPPAPSSPVSKPVFKPVRNIGDGSYVVEFHYNGQNYAWSGSANSPISAMQL
                     AWLTYFG"
BASE COUNT         6615 a         6154 c         5496 g         6642 t
ORIGIN      
        1 tgtttcccgg acatcatccg gtagatggct gtaggggttg ccatccacct tatggctgcc
       61 gctgtaaatc agcgtgattt ccacgccctg tttctccagc gcagcaccgt aattactgtg
      121 agccatcatg acgccgatgg agcctgtccg ggcggtctgc gtgaccagac gccgggaggc
      181 ggcactggca agcagctgac ctgcgctgca gttcatgtca ttggccagcg cccataccgg
      241 ctttatgtca cgcacacggg cgatgatgtc agcgcagtca aatgcccctg ccaccattcc
      301 gccgggcgtg tccatatcca gcagaatgcc gtccaccatc gggtcgctgg cagcctgttg
      361 cagacgggcg ataatgccgt tgtaaccggt catccccgaa tacggctgca gcgcccgcgt
      421 ccggctgacc agcgtgccgg aaaccggcag cacggcgatg ccgttcatga cctgataact
      481 gcgggcctgt cgtggtccgt catcattaac ggataacgcc agcgtcgcgg gtgcctctcc
      541 ggcagtcagg ctgtcaccgg acaccgcatc cgtcaggcgg ctgatcccaa gctggcctgc
      601 aagcgcacaa aagaaaaccc gcgcataggc gggttcaagc ataagcggct cattaaaggc
      661 catgctggca atatgcggga gattacgcag ctctgctgtc actcttctcc tcctctgttg
      721 attgtcgcag tccggattca aatgccgcag ccgcccaggc gggcggttta agaccggctg
      781 cacggcgctc catcgtttca cggacctgct gggcaaaaat ttcctgatag tcgtcaccgc
      841 gttttgcgca ctctttctcg taggtactca gtccggcctc tatcagcatc accgcttcct
      901 gtacttcttt cagaccatcg atggccatac gaccggagcc tatccagtca cagttccccc
      961 aggcacttcg ggcttcctga aaactgaagc gcgcttttga aggtaacgtc accacgcggc
     1021 gagcgatggc ctcttccagc cagcacagaa acatctggct cgcctgacgg gatgcgacga
     1081 attttcgccg ccccataaag tacgcccacg actcgttcgc actggcccgt gccgtggagt
     1141 agctcatctg agcgtaattc cgggaaagct gctcatacga gacacccagc ccggcagcga
     1201 tataccgcaa cagtgactgc tcaaacacgg agtagccgtt atccgtgtcc tgagccgtct
     1261 gcaggttcag tgagtccccc ggcatcaggt gcggcacttt tgcgcctccc agccggaccg
     1321 gtgctgcggc gtaatacgcg gcaatttcac caatccagcc ggtcagcctg tcccgctgct
     1381 ccttactgtt cgcgccaaga ataaaatcca tcgctgactg cgtatccagc tcactctcga
     1441 tggtggcggc atacatcgcc ttcacaatgg cgctctgcag ctgcgtgttc tgcagcgtgt
     1501 cgagcatctt catctgctcc atcacgctgt aaaacacatt tgcaccgcgg gtctgcccgt
     1561 cctccacggg ttcaaagacg tgaatgaacg aagcacgacc gcccggtaac tcacggggta
     1621 tccatgtcca tttctgcggc atccagccag gatagccgtc ctcactgacg taatatccca
     1681 gcgccgcacc gctgtcatta atctgcacac cggcacggca gttccggctg tctccggtat
     1741 tgttcgggtt gctgatgcgc ttcgggctga ccatccggaa ctgtgtccgg aaaagccgcg
     1801 acgaactggt atcccaggtg gcctgaacga acagctcacc gttaaaggcg tgcattgcca
     1861 caccttcccg aatcatcatg gtaaacgtgc gttttcgctc aacgtcaatg cagcagcagt
     1921 catcctcggc aaactctttc catgccgctt caacctcgcg ggaaaaggca cgggcttctt
     1981 cctccccgat gcccagatag cgccagcttg ggcgatgact gagtcggaaa aaagacccga
     2041 cgatatgatc ctgatgcagc tggatggcgt tggcggcata gccgttattg cgtaccagat
     2101 cgtctgcgcg ggcattgcca cgggtaaagt tgggcagcag ggctgcatcc acactttcac
     2161 tcggtgggtt ccacgcccgc aactgcccac caaatccgct gccaccgccg tgataaccgg
     2221 catattcacg cagcgatgtc atgccgtccg gccccagaag ggtgggaatg gtggacgttt
     2281 tcatacataa aatcctgcag gtcccctgcg tcgctgtgtc atgccggtct gcacttccag
     2341 ctccgcaatg tattttttca ggtcagacac ggaagtggcc gtaaactcca ctctccgtcc
     2401 gtctttctgt accgttgcca cccgttttcc tgtcatcagg tcatgcagtg ccgcacgggc
     2461 agcggcaagt tcttcctgtc gcgtcattca tcctctccgg ataaggcacg ggcgtaatct
     2521 gccagtgttt tcttgttggt tgctgcacta tcctcttcct gcagactcgc cagcagcgca
     2581 ctgagatcca gctgccagcg ggaaatactg atacgcagcg ccgccagcgc ataaacgaag
     2641 cagtcgagtg cttcattgcg tcgctttttg ctgtcccaca gtattttttt cctgccatcc
     2701 acccattttt cgacctgctc ttcagccgtc agctgctgcg cttcggtcag atcaaaaata
     2761 tccgggttat tcgggaagtg aacggcaccg ggaagcggtt catccccttc cggcgtcagt
     2821 gtgaagcggt tataaatctg ctctttcgcg gtatccgtac cgatttcggt aaggtaaacc
     2881 ccgtttttgt ttcgcttacg tggcatgctg gccaccggct ttccgtagac ggatgcccct
     2941 ttaatgggga tcacccggaa cagcccatgt tttttcgagc gttcatacac aatggtcggg
     3001 tcaatcccgc caatatccca gcagatacgg gatatcgaca tttctgcacc attccggcgg
     3061 gtataggttt tattgatggc ctcatccaca cgcagcagcg tctgttcatc gtcgtggcgg
     3121 cccataataa tctgccggtc aatcagccag ctttcctcac ccggccccca tccccatacg
     3181 cgcatttcgt agcggtccag ctgggagtcg ataccggcgg tcaggtaagc cacacgatca
     3241 ggaacgggcg ctgaataatg ctctttccgc tctgccatca cttcagcatc cggacgttcg
     3301 ccgattttcg cttcccacgt ctcaccgagc gtggtgttca cgaaggtttt acgttttccc
     3361 gtatcccctt tcgtcttcat ccagtctttg acaatctgca cccaggtggt gaacgggctg
     3421 tacgctgtcc agatgtgaaa ggtcacactg tcaggcggtt caatctcttc accggatgac
     3481 gaaaaccaga gaatgccatc acgggtccag atcccggtct tttcgcagat ataacgggca
     3541 tcagtgaagt ccagctcctg ctgacggatg acgcaggcat tatgctcgca gagataaaac
     3601 acgctggcgg gatcatccgg cgtccatttg aggccaaacg gtgtctcttt gtcgccaaat
     3661 ttaagatact gctcctcccc gcagtgcggg caggcaacat gaaaacgcat aaaatgcggg
     3721 gattcactgg ctgcacgctc aatctggcag gttcctctca ctttgggcgt ggagccacgg
     3781 atggactttg gccagaccga gccttcaata cgcttgtcac ccaggaacgt cggagagcct
     3841 tcctgttcaa tatcatcatc aaaagcagca agttcatcat aacccgccac atccaccgac
     3901 ttttcacggt agttttttgc cgctttaccg cccaggcacc agaaaccacg accattggtg
     3961 aaacgcttca tggtgagcgt gttatcccgg tgctttttgc cataccacgg ggccagcgcc
     4021 agcagcgacg gaatatcacg gatggtcggc tcgacgtggg ttttcataaa gttttcggca
     4081 tcaccatccg tcggcaacca gataagggtg ttacgctgct tatgctctat gaagtaggca
     4141 taaacaccca gcagcatttt ggaataaccg acacgggcag acttcaccac attcacctca
     4201 cggatgtagt cgctgcccat cgcattcatg atggcccgct gaaagggcag tgtttcccag
     4261 cgcccttcct ggtatgcgga ttctttcggg agatagtaat tagcatccgc ccattcaacg
     4321 gcggtctgtg gctccggcct gaacagggca cgaagcccgg cgcggacaaa atgccgcagc
     4381 ctgtcaacct gactgttcga tatattcact cagcaacccc ggtatcagtt catccagcgc
     4441 ggctgctttg ttcatggctt tgatgatatc ccgtttcagg aaatcaatat gccggttatc
     4501 cagctcagga aaacgtcgct gcaccgacag aggaataccg tcgagaatac tggatatttc
     4561 ccttgctata cgcgacagca cgaaagtaca gaatgcggtt tccaccactt cagccgattc
     4621 tttggcattt ttcagttcct gcgccgtcgc ctgagcacga gtcaggcgat ggcgctcaaa
     4681 ttcaagtgtt cctgggtgaa gatctgcctc gctggccagc cgcagttctt caacctcccg
     4741 gcgcagcttt tcgttctcaa tttcagcatc cctttcggca taccatttta tgacggcggc
     4801 agaatcataa agcacctcat tacccttgcc accgcctcgc aaaacgggca ttccctgctc
     4861 ctgccagttc tgaatggtac ggatactcgc accgaaaata tcagccagct gctttttgtt
     4921 gacttccatt gttcattcca cggacaaaaa cagagaaagg aaacgacaaa ggccaaaaag
     4981 cccgttttca gcatctgtcg tttcctttct tttcaggggg tgttttaaat aaaaacatga
     5041 agttacggcg aagaagaacg gaaacgcctt aaaccggaaa attttcataa atagcgaaaa
     5101 cccgcgcgcc ttccgccccg tagcctgccg gatcgccgga aaggacccgc aggcgttctg
     5161 gtttattcgc aatgaattca atgtctgaca gaccagcgtc gtgcgaccac ggtcgcacgc
     5221 tcctgaatac atgccctgtt tcttccaccc ccgcacagag ctggcgagca tgggggacaa
     5281 acccgcgaat cattagcgct gtaaaaaccc ggtatgcatc gtttttgatt atttccacac
     5341 actcgcgcag aggagttccc cgtcaggcta cggtcatagt taatgcggaa atacggcgac
     5401 gatgcagcgc ataatgtgtc aggcttgaat acctttatcc gttaaaaggg atattagtta
     5461 agttatcccg tgtagggtat aagccattat caaagccact cagtagcgaa tggcttttgt
     5521 aatgggctat ttaattagtg atgctgcgta ttcgattaga tataacttag gtgccagcca
     5581 gatttttaac catgtcatat tggctactat agaaataata aaacttaacc atagaataaa
     5641 aactgctact aatggtaaga caaaagcact taacctaccg tcactttccc aaatcatagt
     5701 cggtttgtat ttaggctgtc cattatcaca tgagcgtccg tcattatcga acttaccaat
     5761 ttcaactttt ttgcactgtt tcttcataaa ccaaataacc agcgggattg ttgaaatggc
     5821 tattaatgtt ttaatcagac tatcaactat attccatacc agcaactgat gaacaacatc
     5881 cggaatctga gcctgactga atgcaatggc ggaatcaatt ccatcgctgg cattgttcaa
     5941 taaatcaaca agaattttct ttgcttcttc ttgcatgtat atatcctgta agttgtaagc
     6001 ctgttcgaaa aaataaacaa ctacgagaga cagaaaaaaa aatatctcag gctctctttc
     6061 tataagcttt tagttgagga tgttctgcaa aacaaaactg gttagttatg taactgcttc
     6121 tggaatacaa cagccgacgt gctacaacgt aaaattcgtt tgattgtggc agttgaggaa
     6181 acagacttgc atttctcaat atcatcaatg atgaccacat ccggtacagg catgatgcgg
     6241 gtatgcaatg tttttgcact aataacatca cagccattca gtggacgaat atctgcatct
     6301 tcaggcacta ccagaagcac acgtttctcc gttgcagaaa agccagcagc aagagttcgc
     6361 gacaataaag tcattccact acgtggcttc atacaaattt cagaaatatg atttttttcg
     6421 tccaaatgat gctggccatc atttttatca tcactcaaca actctgctgt taatacaatc
     6481 cctgctgtct cagatttcca ggaaatgctg atatcctgat catctgcatc aacatcatat
     6541 ttcatcgagt aaggactaaa gcaacgtcca acgaacgcat cgtttaccag tacacgccca
     6601 ccctgtctga cctgaaagtc aacgcgccct gaaagctgat ttactgttac aagcaatgcc
     6661 ttttttcttt ttgttttatt actcattgcc acaaactgtt cagccatctc tcttgtcaca
     6721 ctaccagaaa ctctttcttg aattttcaca gtaaattttc tggatgtagt agcatcgaca
     6781 ttaaaaaatc tggatgtcat taggcagtta ttaacaattg gcatcagctc acttacgatt
     6841 gtaacaaatt cccggtaaag ctcattttgc ttttcagcac acagttttcc gcaatcggcc
     6901 agcttaagct caagagcaat taatcgttcc attattgaat taattttttg caactctttc
     6961 atcaaaacct cctgacaaag ataccgtgcg accacggtcg cacgctcctg aatacatgcc
     7021 ctgtttcttc caccctcgca caggactagc gagcatgagg gacaaacccg cgaatcatta
     7081 gcgcggtaaa aacccggtgt gcatcgtttt tgattatttc cgcacactcg cgcagaggag
     7141 ttccccgtcg ggctacggtc atagttaatg cgggaatacg gcgacgatgc agcgcggaat
     7201 ttagcgagcg accacgatcg cacacgagaa acaaagagat ttttatactg cagaggaaca
     7261 agctggcggg taattcagcg tagtgaatac cggcatctcc atcttgtatg cgtaatgata
     7321 ctcagcagtt gctccagcag acgctttcca gccaggaagc atcaaaatag catccgcaca
     7381 acgaagcatt gcaaagcaaa tatccatgta ttcacgttgt gtcagaccat taggcaaagt
     7441 ggctggattc aagacagagt gaccatgtcg tgacaaacga tctgcctctt tattaaaggc
     7501 gtcacgatta aagttctcat atcccgtcat tggcccggca atataaattt tcattcacta
     7561 tccaacaaat tgagttcgtt tttgatacag agggattaat tcaggcattg cgtattgatg
     7621 tattcctgaa gcgttctcag tgctgtttgg tcgcggataa ttccgtcccg gataccgaga
     7681 acgtttcgtc cagcaactga agagagttcg acggtggcat cattgcccat gccggaggcg
     7741 ctggaggttt tggctgaggc tggcacaggg cattttcctt tgacgagcac ccgaccacca
     7801 ttatcaagct tacgccgaag agcatcattt tcagctttcg cattggctaa ctccttcgtg
     7861 tatttagcat cgagtgcatc agcagcacgc tggcgctgct gcatgtcagt aatggtggcg
     7921 gtcgcctgct tcagctctct ggcgtttttg tcgcgctgct ctttgtaggc gatggcgtta
     7981 tcacggtaat gattaacagc ccacgacaag cagacgatga tgccgataac cagagcggag
     8041 ataatcgcgg ttaccctgct cattgttgac cccacaaaca gacttcacgc tcaatctcac
     8101 gacgagtcat caggcctttc cattgcttac cgccagcata tatccagcga cgtagctgat
     8161 cacatgcgcc tttgatatcg ccctggttta ttttgcgaag aagcgtcgat gttctgaaat
     8221 taccagcgcc cacgttgtaa acgaacgagt aaagagcgcc gcgcgttgtt tccggtatat
     8281 cgactttgat gtacggatta atttgtctgg caacagtggc aaggtcttta ttcaggagag
     8341 ctttgcattc tgcttcggta tacgttttac cgagcatgat gtcttttcca gtgtgcccgt
     8401 aacatacagt ccatacgcca acaatatctt tgtatggtat gtaactgaca ccttccagac
     8461 catcgttacc acttgggcca gtgatgagca tagacgctat ggcaatagcc ccgccaccaa
     8521 tagcagctgt gacagctttg cgtaatgatg actgcatcat atttccttcg gtgctctctt
     8581 ccccagctct ccaataacca tcgctgtagc agaaagatta ttggtattag ttttatccaa
     8641 tatgtcctgc aatatcttcg tgcgcttcat ctgctcgcgt ttattgagcc ggtatgtaag
     8701 cacaccgagg ataatgctga acgcgacacc gataatgaag ccccaatcct gcaatgacag
     8761 gctggcaaag aacgcagcaa gaccagcgct accataagat gcactgctgt atcgttcatc
     8821 catcttcata tctcttacct cgcaatagtt gctgaggttt cggcttggaa actatatggt
     8881 gaaaataacc cctaccaaaa acggcagggg aatatattct ccagttactc aaataacttt
     8941 ttcacttcat caactgtctg aagataacgc tcgccctcaa tctcaatacc tattgcctga
     9001 cgtcctaact tcagcgcagc tttaatagtt gagcctgatc ccataaagaa atcggcaacg
     9061 atatctccgg ggcgtgaact actctttata atatgttcca tcatcgctgc aggtttttca
     9121 catgggtgct tgccaggata atattgcaca ggaggaaact cccatacatc ggtataaggt
     9181 acatcagcag taacatgaaa tggacggcgt aaactttcat actgagcctt caaatcatca
     9241 tactgcttct ttacttcctc ataatgcaat ttcagatcgc tataacgctt ttcatgttct
     9301 gaataatcaa cattaaaagg atatggacac gggacaccga gtgaatctgc cttcctgcga
     9361 aataattcgc atagtttatt aaaatcaatt aatgatggaa gccgccactg cgaataagaa
     9421 aaccagtgtg aacacatctg ctttcctgtc gctgaattaa tgtctgcggc tgatatatct
     9481 aactgacggc gagccagcga aaaagcatca attagtggag aaaaaatatt ttttcttaaa
     9541 tctgcacatt ttgaagcata acctgactga cctttagcat aacctgacgc gccgtaatgc
     9601 tctgcaaata atattctttc agtagcagga aaataagccc ttaaactttc tttatgacaa
     9661 ccgcgccaca taccactggg tttagcccaa atgatatggt tcagaacatt gaaacggtcg
     9721 cgagttaata attcaatttt tgacgcacgg cgagagccgg taaacatata caggctgcca
     9781 ttaggggcca atatccgcca aaattctgcc aaaaattcgt caatccaccc caaaaagtca
     9841 gcatcgctat cccactggtt atcccatgcg ttagccttta cgccgaagta tggtgggtct
     9901 gttgcaatca aattgaccga gttatcaggg atggttttga taaattgcag agaatctgcg
     9961 caaacgagtt gtgcgccatg aatattagtg gttttgaaca tagctattat ttttttgcct
    10021 gggtaagcta acccagcgat gcgcatcgcg ggtgggcttt aggttcagcc tatagctctg
    10081 gcatgggttg accgcaggac aagctgcaac ttgtcctgcg cccacttttc aggcacaaaa
    10141 aaaccgccat aaaggcggct tgttggctat tgggtacaaa aaacccaact taacaaaaca
    10201 ataactaaaa accgtcattt tgccaagctt tttcgttttt gtgctgtgcg accgtggtcg
    10261 cacagttttt agaaagttgc ctgcttatat tcatcggtca aagaatatcg accagacccg
    10321 ctacgctttg caaccccaaa acagatcatc tgttcgatga taaattcaac ggcagcctga
    10381 ctcaaaaaac aggcttcgct taactctttc aaggaaatgc gtggatatcc acgcataaca
    10441 cattccacac tcagggccgc ttcggtcata tttccacgaa tttcattaat attcataacg
    10501 cctctctaat caatcttgaa actggaaatc aacatcagcc ataaagcggt tcaactcagc
    10561 cagttttggc cccatagtcc caattaagcg gccagccagt cggtctgtca ggttggtact
    10621 gttgaaacca tattcctttt cgaaacgttc aaccttctgc cacaactcat acaattcgtt
    10681 agctatctcc gcaacgtcgc cgcgcatttt ttcgttacct tgataattca taatatcctc
    10741 gaaattcata tagttagtgg gttatccccc tcaacaacac gaactgtaac tctgccaaaa
    10801 gaacacatcc agtgttattt tcacttttta gtgaaatttt atatttgctt tgttttctcg
    10861 ttttggtgta ttttgcgcgt taggaggcta taatatgttt aacgtgataa cccaccctgc
    10921 agcactggat gaactgcttg aactaccaga cgatctgcga ggtcgcatga ccaggctaat
    10981 tgaacgactg gagaaagagg gaaacaaact taagatgccc catagccgtg taataggcgg
    11041 aggactcttt gaactaagag taggggataa aaacatcgca agaacgctat acgcctacgc
    11101 gactggtaac gaaatttatc tattgcatgc atttgtaaaa aagacacaga aaacaccagt
    11161 aaaggctata gaaatcgcca gaactcgctt gaaggagatg aactgatgaa agcaaaaggc
    11221 atcccgttta acgaagtaaa ggaaaaacta ctcaacactc cagaagctat caaaggatat
    11281 gaagaggccg ataaagaact ggagatggtc gaaatgttat acgaaatgag ggaaagagca
    11341 ggtttaacga agtcagctct ggcagaacga atgggattgc gcccgtccgc catcagccgc
    11401 ctggaaagca atcctttagg ggcaagcatg aaaactttat caaaatatgc ccaagcatgt
    11461 ggcgcaacaa ttaatattaa tgccgtctat tgattaatca cttttcacca ccccgtccag
    11521 aagggcgggg tgtgtatcaa tgatgtaaac actccacagc aatcgcttgt tggcaattct
    11581 gagcaatctc ccgcgacata ctaacaagcc gtgaaatctc gccaagatgc aagctagctt
    11641 cgggagaagc cagagcaacg ctaaccagat ccaaaacggc gtcgatgtct tttaggtttg
    11701 atccgagttc ttcaagagtt gatacagatg cttcagtcat tgaatctaat accatttaat
    11761 taaaaaaggt tatttgatac tgtataaaat tacagggtaa aactcaaatt ttatagccaa
    11821 ctttcaatgg attaacaggg ttaatagctt tgtagtccga tacttatgtt ctcttcctac
    11881 gcctcactca accacctaca gaagcccatc atcaccataa gaaaaactct tttcgcttcg
    11941 ttttctcatc atttcaaaaa ctatgccttt ttgtttattt atcattccgt gaccagtcac
    12001 actttgagca caagtttcag cctcgtagtt gtaaggtgac atgagccttc accatcaaaa
    12061 atacattctg aaacaggcat tttctgacca catcgcgggc aagtagtaga tagctgcttc
    12121 tgcagctcct tatagttttt cctgatcagc agaccgatga cttcattctc tgaatatgga
    12181 actcgtcccg gtctacgcaa tacacaaatt tcccccaaca tacgtagctc gtcaggttcc
    12241 agaatccagt cccgtcttga tgtgccttcc ttcttcaacc gttccctgcg tctcctttgc
    12301 cgttctgcag ctgtcagcgc cataaatcct cctgaaaatt acttaaccca attaactaca
    12361 ccgccagcgg ccacaaccgc tttttcacag tcacgctcat atctcaaagg ttgaaataca
    12421 ccatcaataa aatatttatc tccaccacat gctgcaggaa gtgaaacaga aaactgaata
    12481 tgaccatccg gagttaccgg agagttgcca gcctcatacg cgacccttat ccagtgcata
    12541 agcatttctg tacttacaca tccacaatca acatcaattt tgtcatgctg ctgttccagc
    12601 cattgctcaa aagttaactt gtaagtttgg cttacaggtt tggcttccag ttctgctatg
    12661 tgatttttgg ctgattccaa ctcaatcgac aatttttcca actgctcttt atgcttcttg
    12721 tattcctgat atgcgtgcca agactgacct ttgcgcacac tatccgtgat atcagtaatc
    12781 tgttctggtg ttagcgtggt cagtggctgt gctgggaaaa tcagcacttt cccggaatcc
    12841 caatcaaaac cagcgtgaat tgactgaacc tcaactgaag gtgttgaacc aatgctgcca
    12901 ggcgaatgaa caacgatcgt tacatccata tcgcgacgat cgctgtggtt gttggacaaa
    12961 atacgattca acaaaatacg attcaccaac tcagaaaatt tagaaaattt catgctgatt
    13021 cacctttctc tgctctctcc tgtcggaaca tcactatcat caggtcgcct tttgtcgcta
    13081 tcttggctgt tgtgcctggc tcaatgcggc taagttcaaa tgcgtcatag aacgcctcca
    13141 acgctttctg gcgtttttcc tgtttgcggc gctttcccca ctgctttaga aaaacagagg
    13201 ccgcccactt ccccgcactg aacatgatgt aaaaccaacc gagaagtgcc agaccggtat
    13261 ttagggccgt atcgatcgtt atcgtggtgt cgatattcac tacgccacct cctgaaaatt
    13321 tccctgataa aacgccaaca cacgctgcat aacttcgctc ttccgacaat cgtgacaaat
    13381 catgttcaag cgcctgtcat aacgacgtat ttctccatca ggtaatgacc agataaggtc
    13441 aggatcaaca actaccgatt tcttcggctt tactcttgat agttttttac gggcgttttg
    13501 ccagtccttg cgtgcttgtg cagacagaaa tatcccggag ccagagatat acaaatcacc
    13561 actggcagca agctctcttg ataaacgact aacagcacac cggctaatac cagtttcatc
    13621 cgcaagttgc ctaaccgttc ctcgtccatt caggcgcacg agttccacaa tttgtttatt
    13681 cagttcttcc ctctgttctg gtgtaaaagc ctttgccatt acgattctcc ttgccctttt
    13741 acaagacggg agaaaattgc ggatacatat ttagcctgat gaatagcatc agccagggca
    13801 ttgtgtcgtt cgccatcaaa tggcatatct tttttaggat taaaaccaat aaactgacca
    13861 agattaacga ttgttcgaac atcctgatca ttaaaatatt cccacggaca ggctaagcca
    13921 acgcgctcat aagcaccgcg taaaattaca ttatcgaacg ttgctccatt tccccatact
    13981 ttcaactttt ttaaattaca tgcatggcaa gtaataaagt gtgataattc aaaaagtgca
    14041 tccttaatat caagggcatc attagtacaa atagcggctc tggcttcctt gctctgtttc
    14101 aaccaccata aaatggtatc accatcggcg ataccaccat tcaacatcga gcttctgaga
    14161 ttcactggca cataaaaagt aggacctaat tcctcacttt ctggagaaaa aaacactgcc
    14221 ccgatagaaa caattggagc acatgtgtta gtacccattg tttcaaggtc aatcattaca
    14281 tcagaccgtt tcctgtcgta aataatctga ttagtcacga ataattctcc tcaacgttta
    14341 tctataccca aattttctgt tgatatgttc tggatggttt tggcgttctt tctgattcag
    14401 gtaaatgaac ataaacgcaa tatccatcac ctaaaaaatc agaacgaaca accagaactt
    14461 tcttaccacg ccgccgataa gtatctgaaa gacgctctgc ttcatcatgc gtcatcggcc
    14521 cctgcttaaa tggcgttatt ttcattttct tgtgcgacca cggtcgcacc ccctctgatt
    14581 tccaagtaac gcttaatcca aagattttct atatgcttat tacctggctg atttgataaa
    14641 taccaatcgg caattacaga ctgcttatta ctatctggtc gtgtcctgta accacaagtt
    14701 ggacaccaaa taacatattc atttcttacc cccgaatacc ttaattctgg cttcgacggt
    14761 ttcctgaaca taacttgttt acaaaggcag aatggcactt cctgcccgat ggcgtttgat
    14821 gatttcactg cgcttctccg ccgcatttaa taatgcggtt cgatttaagc tcagacatcg
    14881 cgacttaatt aaaagccagc gttctttata atcttgtctc cagctatcaa tagatatatt
    14941 cagtagaaaa ctcagatgcc tgtcctgttc tctctcatca actgcctcag cacacgtaag
    15001 ttctgattta acctgttgtg ccgcgtaata aatcaatttc cgcatggctt ttttcgtgga
    15061 ggccttcatt tttttaaagc cgccccccga atgagcaacc agaaaatcaa gccataacca
    15121 ctgacaaaca atcacatcat tgttgtaatc aggcttgcat ccgtagcaat aataaagcca
    15181 ggctgattcc tcgttattca actgaccaat agccctgcgc cagctggcgc tctgaaaatc
    15241 aatatcagtg agcaacattg atgattgttt aaatgttttc ccctgatgga atctaaccgg
    15301 ctcagcagga acggacactt cgtatgagtt atcatccccc acactgattg accggactgg
    15361 tttattgtta aaccgtcctg atctaccgag acgcatctgc tcaagctgga cctccagaat
    15421 gccacgctgg cgataatgaa catcagccaa tgccgtagag acgcagctgc gtatatactc
    15481 aagctccacg cttacgactc tccttaatcg actgctttac ccgccgagtt gtaaagtcac
    15541 tcttcagctt ataagcagta cgaacttcaa tatcgctttg ccgcaatgaa ggaatctctc
    15601 cagcggttaa ccattggtaa acagctccgg gtgttacacc tacccctgcc gctgcttttt
    15661 ctacactgcc aaaatggcga ataagttctt ctggtttcat gttgttatta taaacaataa
    15721 acaacaatta aagccagcta taataaataa attttatagc cagctataag aagatcattt
    15781 atgattacag tatgaaaaca cgaggcgaac gactgaaagc acgccgttta gagatgaagc
    15841 tgacgttgcg agaagttgct gaggctgtcg atatctctat agcaggcgtc caaaacttag
    15901 aacgtggcga cgtaatgccg tcactggaga taggtattgc cctggctaag tgcttacgca
    15961 agccagtgca ttgggtgcta tatgggacag aatatgaccc tgatcgcatt cctgttattg
    16021 gcacaactga aagtgggccg gatgaagagt ggaagccggg acaggttcca aatactgagc
    16081 gattcctgcc attcatcagc cagcgcaata ctgtgtatgc gttaactatc agcaatctta
    16141 cgcagggaaa ctatcagcca ggcgattttc tcctggtcga ttctgcgctg gaactggtcc
    16201 ccggcgagga tgttctggtt cgtgatactg caggaaatat cactatccag cgcctggccc
    16261 gttttgatga tgaaaattac tatctggatg gaacaaactc acaacgcgcc atcttcagga
    16321 aaagcgatct tgaatttgtt caccaaatag tcggaacgat caaatcattc atggttgagg
    16381 gtagatgact taatacgggg tttattgttt gctgtaaatc tggtttaatg cagggcatat
    16441 gttctgtggg ttgactcagg ttacagcagc agaaaaaaga cgaaaaaaaa cccgagtcgg
    16501 caaactcggg tccttttcag gaagcagcca cagtaacgcg acggattccg tcaattcaag
    16561 atgcgtttat tgtggctgct cctgcggatt ttttcaaccc gaaaaaacat aattcgcatg
    16621 gaatggctaa aaaatgacat taaacgattt ctatgcggat cgctttggca gcgatccgtt
    16681 ctcgctaatt gaagcagcac gggatgaact aaccgagctg gcacagatgg ctggtatcaa
    16741 ctggcctgcc tgttccgaca acatccaact caaccctcgc gggggggttg agcgttactc
    16801 ttcatacaat aaaacatctc cagaagctct cgaaaagagc ctgaaaggac gcgtagaaat
    16861 ttactccagg ctggaacaca gcaaagggat tgattacccg ttcattaact tcgtccataa
    16921 aggcagcgat gctggctcat ggagcggttt ctccttcctg ttttctgaat atcgccgtga
    16981 gcaacaacgg aataatgcga ccgtggtcgc acaaccagaa gaagaacggg aaagaataga
    17041 acgtcaggcc gaagcacgtc gtcgccgtga agaacaaaaa cgaattaatg acctgaaaaa
    17101 caaccaaatg gaccatgaac gtttgctggg atggctggca ttccacagcg cgtgggaaca
    17161 tgctcaggct gaagacggct catggccata tgcgattaaa aaaggtattc gtgacgtttt
    17221 tagttcatgt gatgtgcgtc gcgtgaccag tcacgacagt gcgaaatgga gccgggggcc
    17281 aaccacatat atggctatcc ctctggctca cctggacggt cgttatgatg gtcgtattgt
    17341 gggttggcag cgaatcgaca aacaaggtgg gaaataccag accagcgcag taaccagcgg
    17401 tgatttcgtc ggtgcctgct ttgttatcgg tgagctgaaa ggggcgcaaa acatcgctgt
    17461 tacagaaggt tttgctactg gtgcatccat ctggcttgcc acgaaaaaag ataaacgctt
    17521 tgacgctgtg gttgtcgctg tatccgctaa caacatggtt catgttgtgg aacaactggt
    17581 aaacatgtac ccggcggcaa aaatcacctg cgcactggat aatgaccgga aatctgccac
    17641 agaaggaaaa gggaataccg gcctgcggac tggctatgaa atcttatcga aattcaacga
    17701 tgtccgctgc gtctatccaa cttttgaaga cgatccgcaa atggagtgca gtgacttcaa
    17761 cgacctgcac aaactacgtg gacttcgtga aacagcacgt caactatttg caacagcaaa
    17821 ccgcctgaaa accggtactg atttgctatc tctgacgctc aaccgcctga agacaattaa
    17881 gcgggataac cgtcgtactt ttgccaaaga gctgctgaga gcggtggata ttggcatgct
    17941 aacctgtccg gttcctaaca gtccgtcaga tttatttaac atgttctgca tcgttctgcg
    18001 tgaaatggga ctggaaaata tctatcgcgc tacggtaaaa gaccacattg cccgtcgcct
    18061 gaataaaaag tgccgtattg cccaggctcc gcgctctttc agcgaacgca taactgatcc
    18121 caataaacgc cctcaacata tcacctataa gcgttttgaa acttcggtaa tgaccgatga
    18181 tgtcctgaag tacgtgcaat cactacaggg gatagtcatc gtccgtgctg gtatgggttc
    18241 cggcaaatct actggattgt tacgaccgtt aatgcatagc tcagaacgtg gtatctctgt
    18301 tgctcaccgt gtctccctta ttggtggcct gtgggaaatg atgacagaag gaaaaggtgt
    18361 aaaagccgat atcctgcact atcaggaccc tggctatcag gaaatggctc catacgccag
    18421 taaactgacc atctgcataa actcgattgt taaaaactgc tggcaaccgt tgatgcgcca
    18481 gcacgactat tttggctttg acgaggccac acaagggcta cgtgccgttc tttccggtcg
    18541 ggctatggaa aacccggttg ctgtattcaa tacgctaatt gacgcgctgg ccagaacgga
    18601 acaacaccca atcatggtgg atgctgatgc gaacgatcta ctggttgacc tggctgaact
    18661 ggctatgaaa cgccgtgaag aactaggttt accggcatgg ctgcagatcc acgtaattga
    18721 attacctgtt gatgttcgca accgcgaaac aggtgagcca atccgggttt tttatacaga
    18781 aaaagatcgc atcatgaccg aggtggtgaa agctgtatcg gatggcgaaa aaatcatgtt
    18841 ggccaccgac agctccacct ttgcagaaga tgtgacagcc accctgagaa tgcattttcc
    18901 tggtaagaaa tttctgtgtg taaaccagaa aaacaaacca gagccagaag ttgaagcatt
    18961 taccaatcaa ccgaaaaaga tggtcaaaaa atacgacggc ctgatttata gcccgtctat
    19021 ttcatccggc gtgtcgattg agcaaaaaca ttttgaccgt catttcggca tgttctgtgg
    19081 tgaagttgta cccagcgacg cagtgcaaat gctacgccgc gaccgtacag caaaagaatt
    19141 tattatcggc tttgataaag ctcgtgcaaa acgtgaaact gacccgcaaa aaatagaacg
    19201 agcattcgcc caggcattgc ttgctaccgc aggtatgaac ggtgaactta cggatgtcgt
    19261 tttcgatggc gaccgtatat caatgggggt ggccaatacc gactttacca ggatgaaaat
    19321 taaagcagcc gcaattgaag cgactgcgcg taacgactac gccagcaaca tgatctgcat
    19381 catgcacagc gacggttata aggtatcacc gctggccacc gattatgctg cgaatgatat
    19441 cggtaaagat ctgcgtaaag aagcgcggga tattgtgtgg gaacagacac tggatctgca
    19501 cctcaataca gaaactccac aagaatcaga gcgtgaagaa ctgctaaaaa aacgcgcgct
    19561 gacgcttgaa gaacaggcaa aacttgtccg ctgggatatt gaaaacgaac tgaaactacc
    19621 ggttgaagaa ggcacgctga aattctattt cgatggtgct cgagacaaag ttcgccgcta
    19681 tgaaacaatg cttttagatg aggtaacagc tcgtcgttac gaccgggaag aagcagccat
    19741 caattttacc tacgcatacc gtcaggcggg acagtggcaa tattttgtag tgaccgcgat
    19801 gactcgcgag caggctgatg atgtctttaa aactaaattc cctggaataa tcgatcacaa
    19861 ggtaaaagct actcctactg ttgaagtcgg ggcgcgcggt ttctatgcgc ttaaatcagc
    19921 aactctccgg cagtacttta tcgactgcgg tatcaaccct gagactatga ccggcgaggc
    19981 gactcaggag cgtctcaaat tcgctcgaga taacctcatg acagccgaac gccgtgacct
    20041 gttgaataac gttctgcaca ttggtggttt tatgacgcca aaagggaagc ctaaagtgcc
    20101 ggaagcgttg tttaaaaaca tttgcgactc tcttggcctt aaaacaggta aacgtcgcgg
    20161 cagagacggc gataagcgtc caaccttgcg ttttgttgag cctgaatcag cggcatttat
    20221 gctggatatt attgttcagc gtcagaaaga tggcctaacc ctaaaaacgc gtaaagccga
    20281 gaagacagcc atcgaaatgg atcgcgattt ggatctcaat atatatatgg atgataaaac
    20341 gcgatccaca aacgagcagg ttcaaaacgc ccctcattca gtaatttctg aggctttggc
    20401 cgtgttgcca gtggctgtac ctgagtcctg ggcgatgagt gcgctttcca gtgaggagtt
    20461 ggccactatg tcatcatggt catcagccag catagctatg acgtttgcat ccctgtacct
    20521 cacagagttc atggatcacc tttccagcaa tgagctgcgg cggttacgtg aatacattac
    20581 ggggaatagc tcatgcagct acgaagagca gggcgctttc tatgggtaat gagtggatat
    20641 tatctttgtg tctcttgctt tgtgtgttcc agcaatggtt ttggttcata gaccgaaagt
    20701 ctcacaagga aaacatgaag gccaggaagg cgatgtttcg tggtgaacaa tttgcgcaga
    20761 cttatcgtat gtggaaacgt ttctatcgtg aattttcggt gaacggggag taaaaatatg
    20821 tctactactg catttttgga caaacttgat taccgtcagc tgcagttctg ccgggatgaa
    20881 tgcgaacgac gcattagggc tatccaggag gaagaaaaaa aggtcgcctg ggctgtaaca
    20941 gatcgtggcc ttatctttgg ctggttccgt acggaggatt atctgaaagc agtggaatta
    21001 ttggcgcgta aggctgctga gcgttgggaa cgagccgaca aggaaaaccc ggagacgaag
    21061 tatgaactga atctctacat cgagggcaac cgtctaccat tgtcagagta taacgctttg
    21121 tttgctgatg gtgagtgggg ttaaaaatgg ctgttcaatc atacaaatac gaatcccctt
    21181 tggataaggg atacattcgt atcaagttaa gtcgttatca gcataaggct atttttaagg
    21241 taaggaaaat tcgcatattg gatgcgtatg cgtattacta caacggtgaa aatgttgttg
    21301 ttgaacactt tcttgcacga tggtttgtag ctctgatttt tgtccctctc ctgttaattg
    21361 gtacttttgt ggatggtttt cctagtacat ggcgggagat aaagaaaggc ctattcccgc
    21421 gtaagtacgg cagattttcg caagatagct ggcgagttat acctggtaaa cattctgtag
    21481 atgagcaacc gattgttgat ttctggttga atcgtatggc tgatgctaaa aaagttgatt
    21541 gaattgtgat gactgttaca ggagataaag tgaagagttt tatttttgtt ttatgttttg
    21601 ccttttctgc tgcggtttat gcccaggaag atagcatttc tgttattaac tcatcagcgg
    21661 accttgcatg taaaaataat cccgatgctg catcaaaaga gtcatgcaaa gagctgctcc
    21721 tttcgacggc tgcttttgct tctgaaaatg caaaatttta ttcagccttt tgtcgcggct
    21781 atgttaagga cgaagataaa gaaaaatgtg atgatgccgg taagttagtt aggtatttcc
    21841 aatctcttaa tcaataatgg cctttgatta acgatcaact tctgatcact cgttcaagca
    21901 aactataaaa acacctgtcc ccgtgactgg tcacggggta aaatgtcttt gcaaattatt
    21961 gacgtgcgcg tcgttttgac ggtaaagttt actccgctac ggtaaatccc gtagccgggc
    22021 gtaggaaccc gagtaaatga agcgcacaac acgcgccagc gtgttttttt gtgtgtaaaa
    22081 tctgcgtaca ccaaatttat ggtggctcag atggggcagc ttttagctgg ccggtttctt
    22141 cgtttaccgg tattcctacc cccgtctggg ctaccgccat atagagcgta ggaactctgg
    22201 tggtagcacg accaattaaa cggagtgcat accatgttca aattcaaatt tgcggcaatc
    22261 gttcgcacag acaaaaagtc acacatccat catctttcca ccatcgcgtc atctgaacgg
    22321 gaagctcgtc gccagttcgc cagccggttt gtcctggtat tgtctgcccg tattccggtg
    22381 cgcgaggtgg ctgcatgaat caggtaaaaa tgaacactgt tggcctgctg gaatcgttgg
    22441 atgaacgtct ggctcaggtc tatgcgctcg tatcatcagc ccatcggaca atctccagtt
    22501 gtgaggcgtc gatttatctc tcagaagcag ggaaactcct tcaaattgcg tgtgagctaa
    22561 cacacgaagc acagggttat tcagcatcgc tctcgtcgca attgctgcac gataaggagg
    22621 ctgaatgagc gcgttagcag tcttttcctt tcaggaagag catcaggttc gtgtagtgat
    22681 gattaacggc gaaccgtggt ttgttgcatc tgatgtgtgt atggcggcgg gtattgattc
    22741 tacggccatc agaaagttgg atgaggacga aaaggggcag gcgaggtaga ccacacaaca
    22801 aggcaccgat aattcggtgc ctttaccgta tgctttatcc gaaatatgtc agccatgcta
    22861 actgcattgc tgatataggg ctgtttgcac tgccggacca agcataattc tggccattat
    22921 aatgaaactc aacaacgtat gagccatcac caatatttct cacaggctta aataccggct
    22981 tagatacagg tgaagaaggc gcgggcggtt gctgttcttc tacgctctct tcctcttcct
    23041 cttcctctat ctcaatttcg tcgtcatcca gatcgtcatc ctctgataat tcatccgtct
    23101 gttcaggtat tgcttccagc tcttcttcgt caggcataac gatttctgga gaatctattt
    23161 tcagctgcca ttgtccattt tcgccaacaa attgccccag agcatcagca gcaaattcca
    23221 ggtatcgact aatcattgta gggctaaatt tgaatgctct tagagtgctg ttcgtgattt
    23281 ttatagaggg gtcatgctct accagctgct taactgtgtt atgcagacga accccggcat
    23341 ctcctctggc gaatcctggc atttcgtcat caagctgctg caatgcttcc agcctggtat
    23401 tttcgtttcc agtatttggc ctccatgtct ttgagaagtt ggccagcttg aattgcttat
    23461 agtgtaattg agtgttttca tcgtcatggc ctaaaatttc cataaagaaa acatcctcat
    23521 ctacgttttt ccaacgagga tctacgcgga aaaacatttc atacgcaata cgggcataaa
    23581 tagcacgact atctttataa acacggcggt catctttgaa aaatgatttg acccacgggt
    23641 taaaagcctt tgctaatatg gcattaatcc gtccgttttc agagcgtgtg tcgttatctc
    23701 cataaccttt aacgacatca tcaaaatcag atgctgctgg gcatgagcga agtacattta
    23761 ttaaataaac aaatgttttt gcttcgcata atgtatatat ttttctggtt atatcatcag
    23821 aagttctttt ttttgcttgg cctaaaaaat caacggtata cttaccagaa acagtaaatg
    23881 taccttgata cataatctca atcattcgtc ttcctgacaa tgcagcaagt gcaaatgcca
    23941 aaggagccat acctgaacga gtgtttagat tatttaatgt agcagggcta tttataatat
    24001 cgtaaatagc ttgcatatat ttagggtaat caatgaccac cacgttacgt ttcttattgt
    24061 gcagaacgtc cgcccatcgt tgctggatag atgcgcgttc ggcagggctt agttgaagat
    24121 gatagagaat ctcatggtta acttttagct gattcaactc ttcaagtaag gcagatcctt
    24181 gctgtagtaa tttatataaa taatcgcgct tatctttcca gttatcgcta gttaaatcag
    24241 atatggcgaa ttgccactct ttatatttat ttgataattt taataactta ttatgacttt
    24301 tgttatcacc aattctgata ttagataaat cttctgataa agggagaatt aattttaatt
    24361 ttgcctgtaa agctgacatt ctctggcgaa tatcggcagc aggtagagaa agccatgaca
    24421 atagctcctc attatataaa ggatactttt cagataggcg atgtatattt ttttcaaagc
    24481 tatgatgaag tttatcatca aaccgttttc tggccctgct catataggca ttgaatgtgt
    24541 tggctgtaat tcttttttct aatcctttcc ccctaaactt tcgcttatca ttaaataatg
    24601 catttttaaa ttttaatgca acagccttta ttttttttgt tttttcccct tgaggacggt
    24661 cagagttatc tattgactca acatcgctaa caagtgagtt tattaattca ccgattttta
    24721 ctttgctcat ctttcctctc ctgattatca gctctgatta tcgtaacaca atttgagaat
    24781 aagataacta ataacgcgtt aacttagcca caaactaaca gaacataatg acggataata
    24841 aacaaaaaca caatatcgga acgttaaaat ttgttccttg actttgttct aaaaatcagc
    24901 acataat
//
LOCUS       DAZOZY010000044        24783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZOZY010000044 DAZOZY010000000
VERSION     DAZOZY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.044"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(54..344)
                     /gene="ubiK"
                     /locus_tag="ACHURQ_001546"
     CDS             complement(54..344)
                     /gene="ubiK"
                     /locus_tag="ACHURQ_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR0823358.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
     regulatory      466..614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            718..1371
                     /gene="ribB"
                     /locus_tag="ACHURQ_001547"
     CDS             718..1371
                     /gene="ribB"
                     /locus_tag="ACHURQ_001547"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR0823359.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1632..1802
                     /gene="yqiD"
                     /locus_tag="ACHURQ_001548"
     CDS             1632..1802
                     /gene="yqiD"
                     /locus_tag="ACHURQ_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR0823360.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1860..2633)
                     /gene="zupT"
                     /locus_tag="ACHURQ_001549"
     CDS             complement(1860..2633)
                     /gene="zupT"
                     /locus_tag="ACHURQ_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR0823361.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2776..3564
                     /gene="ygiD"
                     /locus_tag="ACHURQ_001550"
     CDS             2776..3564
                     /gene="ygiD"
                     /locus_tag="ACHURQ_001550"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR0823362.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3602..4762)
                     /locus_tag="ACHURQ_001551"
     CDS             complement(3602..4762)
                     /locus_tag="ACHURQ_001551"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0823363.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(4768..5439)
                     /locus_tag="ACHURQ_001552"
     CDS             complement(4768..5439)
                     /locus_tag="ACHURQ_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR0823364.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5587..7068)
                     /gene="tolC"
                     /locus_tag="ACHURQ_001553"
     CDS             complement(5587..7068)
                     /gene="tolC"
                     /locus_tag="ACHURQ_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR0823365.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7273..7902
                     /gene="nudF"
                     /locus_tag="ACHURQ_001554"
     CDS             7273..7902
                     /gene="nudF"
                     /locus_tag="ACHURQ_001554"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR0823366.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7903..8325
                     /locus_tag="ACHURQ_001555"
     CDS             7903..8325
                     /locus_tag="ACHURQ_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR0823367.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            8350..9177
                     /gene="cpdA"
                     /locus_tag="ACHURQ_001556"
     CDS             8350..9177
                     /gene="cpdA"
                     /locus_tag="ACHURQ_001556"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR0823368.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9177..9758
                     /gene="yqiA"
                     /locus_tag="ACHURQ_001557"
     CDS             9177..9758
                     /gene="yqiA"
                     /locus_tag="ACHURQ_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR0823369.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9787..11679
                     /gene="parE"
                     /locus_tag="ACHURQ_001558"
     CDS             9787..11679
                     /gene="parE"
                     /locus_tag="ACHURQ_001558"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR0823370.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11727..12041)
                     /locus_tag="ACHURQ_001559"
     CDS             complement(11727..12041)
                     /locus_tag="ACHURQ_001559"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR0823371.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12072..12653)
                     /gene="mdaB"
                     /locus_tag="ACHURQ_001560"
     CDS             complement(12072..12653)
                     /gene="mdaB"
                     /locus_tag="ACHURQ_001560"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR0823372.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(12763..14112)
                     /gene="qseC"
                     /locus_tag="ACHURQ_001561"
     CDS             complement(12763..14112)
                     /gene="qseC"
                     /locus_tag="ACHURQ_001561"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR0823373.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(14109..14768)
                     /gene="qseB"
                     /locus_tag="ACHURQ_001562"
     CDS             complement(14109..14768)
                     /gene="qseB"
                     /locus_tag="ACHURQ_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR0823374.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            14920..15312
                     /gene="ygiW"
                     /locus_tag="ACHURQ_001563"
     CDS             14920..15312
                     /gene="ygiW"
                     /locus_tag="ACHURQ_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR0823375.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15365..15847
                     /locus_tag="ACHURQ_001564"
     CDS             15365..15847
                     /locus_tag="ACHURQ_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR0823376.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            16393..18651
                     /gene="parC"
                     /locus_tag="ACHURQ_001565"
     CDS             16393..18651
                     /gene="parC"
                     /locus_tag="ACHURQ_001565"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR0823377.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            18884..19621
                     /gene="plsC"
                     /locus_tag="ACHURQ_001566"
                     /gene_synonym="parF"
     CDS             18884..19621
                     /gene="plsC"
                     /locus_tag="ACHURQ_001566"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR0823378.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            19696..21108
                     /gene="ftsP"
                     /locus_tag="ACHURQ_001567"
     CDS             19696..21108
                     /gene="ftsP"
                     /locus_tag="ACHURQ_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR0823379.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21219..23438
                     /locus_tag="ACHURQ_001568"
     CDS             21219..23438
                     /locus_tag="ACHURQ_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR0823380.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(23481..23738)
                     /gene="yqhH"
                     /locus_tag="ACHURQ_001569"
     CDS             complement(23481..23738)
                     /gene="yqhH"
                     /locus_tag="ACHURQ_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR0823381.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(23789..24715)
                     /locus_tag="ACHURQ_001570"
     CDS             complement(23789..24715)
                     /locus_tag="ACHURQ_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR0823382.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
BASE COUNT         5787 a         6233 c         6634 g         6129 t
ORIGIN      
        1 cactgtaaat tagaagatta attcataatt tacagtgcat taaagacaaa tgtttacagc
       61 gttgggggga gagtctctgg atctggctgc tttttaatct cagtactgcg attttccagt
      121 tcgctcattc tttgttcaag ccgcgccagt ttctcacgag tccgtaacag gacttgcgtt
      181 tggacgtcga actcttcacg gcttacaagg tcgaggcgcg tcagctgagc ttgtagggtt
      241 tggcggattt ttttctccac atcttccccg aactccctga ttcctttagg cattgattcg
      301 tgaacctggc gagcgatttg ctcaattttt ttcgggtcaa tcattgtagt ttccctgtac
      361 tgataggtgt tgagtaccat tgtagtgcga taagggtaag tcataaacca gaattatgtg
      421 aagctatgcg ttgctgccgc taatcattag cgttatagtg aatccgctta ttctcagggc
      481 ggggcgaaat tccccaccgg cggtaaatca actcagttga aagcccgcga gcgctttggg
      541 tgcgaactca aaggacagca gatccggtgt aattccgggg ccgacggtta gagtccggat
      601 gggagagagt aacgattctg tcgggcatgg acccgctcac gttattttgg ctatatgccg
      661 ccactcctaa gactgccctg attctggtaa ccataatttt agtgaggttt ttttaccatg
      721 aatcagacgc tactttcctc ttttggtacg cctttcgaac gtgttgaaaa tgcactggct
      781 gcgctgcgtg aaggacgcgg tgtaatggtg cttgatgatg aagaccgtga aaacgaaggc
      841 gatatgatct tcccggcaga aaccatgacc gttgagcaga tggcgctgac cattcgccac
      901 ggtagcggta ttgtttgcct gtgcatcact gaagatcgcc gtaaacaact tgatctgcca
      961 atgatggtag aaaataacac cagcgcctat ggcaccggtt ttaccgtgac cattgaagcg
     1021 gctgaaggtg tgactaccgg tgtttctgcc gctgatcgta ttacgaccgt tcgcgcagcg
     1081 attgccgatg gcgcaaaacc ttctgatctg aatcgcccgg gccacgtttt cccacttcgc
     1141 gcgcaggcag gtggtgtact gacgcgtggc ggtcatactg aagcgactat tgatttgatg
     1201 acgctggcag gctttaaacc ggctggtgtg ctgtgtgagc tgactaatga cgatggcacg
     1261 atggcgcgtg caccagagtg cattgagttt gccaataaac acaatatggc gctcgtgact
     1321 attgaagacc tggtggcata ccgtcaggca catgagcgta aagccagctg aaaaccgctg
     1381 cttaattact gccttaatca agaaaccgaa gttgtagctg gcttcggttt ttattttgcc
     1441 ctgctatgca agatgttaat tccgcttctc tttctatgag aaaatttcat taatatcagg
     1501 cattcttttt cattatgatc aaaatcatat aattgttccg ttattatgcg accatatgaa
     1561 tatatccgaa aaaggatatg tgaaatttat gcgctaaatg tttagtattg ttaaatatta
     1621 agggtaaaag gatgtttatc gcctggtact ggattgtatt gattgctctg gttgtggtgg
     1681 gttatttcct gcatttgaaa cgttattgtc gggcgtttcg ccaggacaga gacgcactgc
     1741 ttgaagcgcg gaacaaatac ttaaacagta cgagagaaga gacagccgaa aaggtagaat
     1801 aaggctttcg tgaagctggc agaagttgtc catgtgctgg acaacttctg atgttgtttt
     1861 taaccaattc ccgccgtttg tagcagcact aaactgaatc ccatcactga cattccacat
     1921 agtacgccat agctggggtt attattaggg tcaatctctt tggcgagcgg cattaattca
     1981 tcaaccgaga gcgccaccat aattcctgca accgccgcca tgattgccgc catgaccacc
     2041 ggggaaatca tgctaccgag gattaaccac gccagcacac caccaagaat ttctgctagt
     2101 ccagaaatcc ccgcccacag aatcgcggtg cgtttagacc ccgttgccgc ataaaccggg
     2161 cctgccactg ccagaccttc agggatattg tgcaacgcga cggccagtgc gatgccaaat
     2221 cccagctcca ggttgctgct cgccgtgaca aaggtggcaa tcccttccgg gaagttatgc
     2281 agactgatgc cgagagtgag cagaatggct gtgcgcttga tcgattttgg caacggctgc
     2341 accgattttt gcattaaatc ctgcggatgc gcatgtggca acatgcggtc caggccaaaa
     2401 tagccaagca gaccgaagat aaacattcca taacccaaca caggcgacat tccttcagct
     2461 gctagtgcgg caggaagcat ttccattaat gagatgagca acatgatccc cgccgcaaat
     2521 cctagcgaaa acgccagtaa gcggttcgag ggtttttgcc cgagaacgcc gagaaacgcg
     2581 ccaataaacg tggctgcccc cgccagtatg gtcagaatga gaggtactga catcgacaac
     2641 tccttataaa tcttcacctt caggcagatc gtcaaaataa ctgatgatca tcatcattgt
     2701 tatccgggtt cttactccgc cgaacaagcg tattgtgagg atatcaaaat gacaccttta
     2761 gttaaggata tcatcatgtc ttcaacacgt atgccagcat tgtttttagg tcacggtagt
     2821 ccgatgaacg tgctggaaga taatttgtat acccgcagct ggcagaagtt ggggatgaca
     2881 ttgccacgcc cgcaagcgat tgtggtggtt tcggctcact ggtttacccg tggaacagga
     2941 gtgaccgcga tggagacgcc gcccacgatt catgactttg gtggcttccc gcaggcgctg
     3001 tacgatacgc attatcctgc tccgggttcg cctgtgctgg cacagcatct ggttgagctg
     3061 ttagcgtcgg tcccggtgac gctggataaa gaagcctggg gctttgacca cggttcctgg
     3121 ggcgtgctga ttaagatgta tcctgacgcc gatatcccga tggtgcagtt gagtatcgac
     3181 agtagcaaac ctgccgcctg gcatttcgaa atggggcgca aactggcagc gctgcgagat
     3241 gaaggaataa tgttggtcgc cagtggcaac gtggtgcata acctgcgtac agtgaagtgg
     3301 cacggtgata gttcaccata tccttgggcg atgtcgttta atgagtatgt gaaagcgaat
     3361 ctgacgtggc aaggggcggt agagcagcat cctctggtga attaccttga ccatgaaggt
     3421 ggcgcgttat cgaacccaac gccggagcac tatctgccgt tgttgtatgt cttaggtgcg
     3481 tgggatgggc aggagccaat taccattccg gtcgatggta tagaaatggg cagcctgagt
     3541 atgctgtcgg tgcagatagg ttaacaatta ccggatggcg aatgccatcc ggcatcattg
     3601 attactcaat aaaaatatgt ggataaaacc gagacatatc ctgggtgatc aacgcacggt
     3661 cttcacgaat gccaattccg gcaggttgat cgttcaccag ccagctacca atcagcatat
     3721 agctgtcgcc gaatttcggt aacgggtgga attgctgaac aatcatccct tcttcgccat
     3781 acggaccttc cgctgcttca atggttttgc cgttctcaat gatcgacacg tttgcgcctt
     3841 cacgggagaa gatcggttta accacatatt tttccatttg cggatgatca tcttccgcaa
     3901 aataagcggg cagcaggttc gggtgattcg ggaacatctc ccacagtagc ggtagaagcg
     3961 ctttgttgga gataatgctc ttccacgccg gttccagcca gcgtacgcct gcatcctcca
     4021 gcttggtgga gaacatctca cgcaacataa attcccatgg atacagcttg aacaggttgg
     4081 aaattacctg atcctgtaaa tccgtgaact gacctttttc acctaacccg atatcgtcaa
     4141 tgtagaggaa ctcggtagcg atttcagctt ccgtcgcgca gtcctgcaaa tactgaatgg
     4201 ttccgcgatc ttccaccgtg tcgcgacagc aggtgagatg cagcaactgg aagccatact
     4261 gttcacgcaa ctcgacaaag cgatcgatta gcttttcttg cagactgtta aactggtcgc
     4321 tgccctccgg caagttaccg gcgttaagct gatcttccag ccagatccac tgaaagaacg
     4381 ccgcctcgta tagtgacgtt ggtgtatcgg cgttattttc cagcagttta ggttcgccag
     4441 tgccatccca tgccagatca agacgcgaat aaagcgatgg ctggtgagtc agccatgact
     4501 gacgcacaaa actccaggtg cgttttggaa tgcggaattt ggtcatcagc tcatcgctgg
     4561 cgatcacttt ttccaccact ttcaggcaca tctggtgcag ttcggcggtg acttcttcca
     4621 gcttttcaac ctgggcgagg gtcaacttgt agtaagcatc ttcacaccag tacggctcgc
     4681 cgtacatggt gtgaaaattg aaaccgtatt cgtgggcttt ttcgcgccag tccgggcgct
     4741 cggtaatact gactctttcc atcggtatca gccacccatt gaacgagaag aggtgccggt
     4801 tgcgctacgc tgcatagtgc tttgtttggc aacagattca ccaaaaccgc cacgggtaac
     4861 ggtagtagtg gtcgccggtt ttggtgccat ggctgtcttc ggtacggtca tggtgcggcc
     4921 tggctgggct gcgccatagt ttttacccgt cgcgtcggta tatttaccgt aagccgggct
     4981 ggctgggttt ttcgaggaga acagcggctg ctgtgcaaat cccgcgccgc cgcccatcag
     5041 acgccccatc atgtaaccgg ccatcagcgg catccagaaa ctcccgctgg attgctgggc
     5101 ctgcgcctgg ttttctggtg ccatgccagc ctgggctggt gcctgctggc actgaccttc
     5161 accaaattca gcaacacagt cttcacgggt ggcgtatttc ggcgcagtac gttcggcttc
     5221 tttcagcgca ttgttgtacg cggtggtaca ttcggcgctt ttgcctgggt ttgcagctga
     5281 acagtcgtca gcattttgat agagagacac tgtttcatca ctcttttcac agccagccag
     5341 cataaaaaca gtggcaaccg cgagagcgac tggtgtcaga tggcgtgcgc tccagttttt
     5401 gcggaacgat gcgtggcgta tggattttgt ccgtttcatt tttgtcttcc gggaccagtg
     5461 gtaaataccc atcagaatag aggatggctg gtcgaaattg aagcgagaag ggggaagaat
     5521 gcggcagata acccgtatct ttacgttgcc ttacgttcag acggggccga agccccgtcg
     5581 tcgtcgtcag ttacggaaag ggttatgacc gttactggtg gtagtgcgtg cggatgtttg
     5641 ctgaacgact ggtgccgggc tatcaggcgc ataaccatca gcaatagcat tctgttccgg
     5701 cgtttgcggt gcaacgtttt ccggattagt ggaaaccggt ttgctcagcg cattgttcag
     5761 tgccagcaga tcctgctcgt tcaacgtacc cagagctgac ttaatattca actggttaat
     5821 caggtagtta taacgcgcat ttgccagctc ttgcttagcg ttgtacagcg tggtggttgc
     5881 atccaacaca tcaacaatgg tacgcgtacc gaccgagtag cccgcttcca tcgcgtctaa
     5941 tgagctttga gcggaaacta cggcttgttt gtaggcgtta atgctactga tagatgcatt
     6001 aatgttgttg aaggaggaac gtacggtttg cacgacgcta cgatgcgcgc tttccagttg
     6061 ctcgctggca ccaacaaagt tgtactgggc ctgtttcacc tgcgagttaa ccattccgcc
     6121 ctgataaatc ggcagcgaga agctcaggcc cactttgttc tggcccatat tgctgtcgtc
     6181 atactgggta ccagcggcac cacgggtttt cgaaccgcta taagaggtgt cagaaatccc
     6241 ggtagaagcc gttaaatcca gcgtcggtaa gtgaccatcc tgcgcctggc gaatttgctc
     6301 gcgcgccagg tcctggctca agcgtgcctg taacagcgac aggttgcgtt tttcggcttc
     6361 tttcagcagc gcgttaaccg gctgtggttt gtcggtttta aagttttcga cattcagcgc
     6421 cgccagttcc ggatagtagt taccggtgat ctggcgcagc tgctctaccg cgttatcaag
     6481 gttattacgt gcggtcactt cgttcgccag cacggtatcg tactgcgcgc gggcgttctg
     6541 cacgtcggtg atcgctacca ggcccacgtt aaaacgttgg gtggtttgat ctaattgacg
     6601 gtagatcgct tctttttgtg cctgtgtata ggaaagaacg tcaatagcat tcaacacgtt
     6661 gaaataagcg gtcgcggtgt tgaggatcaa ggtttgctga tcggtctgat atgtgacgtc
     6721 ctgaatccct gctgcttttt cctgcagcgt taacgcacgc catttcgaca tatcaaaaat
     6781 ggattgagtt aactgcaggg acgcactggt cgcgttagag ttgatgccgt tcgcgtcgcg
     6841 gtagccgttg ctataggtgt aatctgcacc taaacctagc tgtggcagta atggactgcg
     6901 cgcttcatta attttttcaa aggcagcatc acgatcggcg gcagacttac gcaattccgg
     6961 gttactaagg cgtgcttgct gataaacttg catcaggttc tcggcctggc tcaacgaact
     7021 gaacccagaa aggctcaggc cgataagaat ggggagcaat ttcttcattt gcattccttg
     7081 tggtgaagca gtatttagcg cgatcaaact gtaaaattat tgcggattct agcagaagcc
     7141 gctaccgcaa aaggttggcg ttacgtgcca tagggcgtta atgtgcctca atttaacaca
     7201 tgactgctca aacggcagtc aaacgtcgct gaaattcaca tttaattcac tattagtgcc
     7261 aggacattaa caatgcttaa gccagacaac ctgcccgtta catttggcaa aaacgatgta
     7321 gaaattattg cacgagaaac actttatcgc ggcttttttt cattagatct ttatagattt
     7381 cgtcatcgtc tattcaacgg gcaaatgagt catgaggtac ggcgggaaat ttttgagcgc
     7441 ggtcacgccg cagtcttact accctttgac ccagtgcgcg atgaagttgt gctgattgag
     7501 cagattcgga ttgccgcata cgacaccagt gaaaccccct ggctactgga gatggttgcc
     7561 gggatgatag aagagggtga aagtgtggaa gatgttgccc gtcgcgaagc gattgaagag
     7621 gcgggactga tagtcaaacg gaccaaaccg gtgttaagtt tcctggcaag cccggggggc
     7681 accagtgagc gttcgtcaat tatggtgggc gaagtggacg ccacgaccgc aagcggtatt
     7741 catggtctgg ctgatgaaaa cgaagatatt cgcgttcatg tggtaagccg ggaacaggca
     7801 taccagtggg tagaagaggg gaaaatcgac aacgcagcgt cggtcatcgc tttgcaatgg
     7861 ctgcagctgc atcatcaagc gttaaaaaat gagtgggcat aaatgaagcg ttacacacct
     7921 gactttcctg aaatgatgcg cctgtgcgag atgaactttt cacaattgcg ccgtttgtta
     7981 ccgcgcaatg acgcacccgg cgaaactgta agctatcagg tggcaaacgc acaatatcgg
     8041 ctgacgattg tggaatcgac ccgatacact accctggtga caatagaaca gactgcgccc
     8101 gcgatcagtt actggagcct tccgtcaatg acggtgcgtc tgtatcatga cgcgatggtg
     8161 gctgaagtgt gttcaagcca gcagattttt cgcttcaaag cgcggtatga ttatcctaat
     8221 aaaaagttgc atcaacgcga cgaaaagcat caaattaatc agtttttagc ggactggttg
     8281 cgatactgtt tagcacatgg agcgatggcg attccggttt actagcatcg tgaaacctaa
     8341 ggacaccatt tggaaagcct gttaaccctt cctctggctg gtgaggccag agtcaggatt
     8401 ttacaaatta ccgacactca cctgtttgca caaaagcacg aagctctgtt aggggtaaac
     8461 acctgggaaa gctaccaggc ggtgctggag gcgattcgtc cacatcagca cgaattcgac
     8521 ctgattgtcg cgacaggtga tttagcgcag gatcaatcct ctgcggccta tcagcatttc
     8581 gctgaaggca tcgcaagttt tcgtgcgccc tgcgtctggc tgccgggcaa ccacgatttc
     8641 cagcccgcga tgtacagcgc gttacaggat gcgggtatct ccccggcgaa gcgggtgttt
     8701 attggtgagc aatggcaaat cctgttgctg gatagccagg tgtttggcgt gccgcacggt
     8761 gagctgagcg agtttcagct tgagtggctg gaacgtaaac tggccgatgc gccagaacgc
     8821 catacgttgc tgctgctgca tcatcatccg ctacctgcgg gttgtagttg gctcgatcaa
     8881 cacagtctgc gtaacgcggg cgaactggat accgtgctgg cgaagtttcc gcacgtcaaa
     8941 tacttgctgt gcggtcatat tcatcaggag ctggatctcg actggaacgg tcgccgcctg
     9001 ctggcaacgc cgtcgacctg tgtgcagttt aagccgcact gttccaactt tacgctggat
     9061 actatcgcgc ccggctggcg tactctcgag ttacatgctg atggcacgct gaccaccgag
     9121 gtgcatcgcc tggcggacac acgtttccaa cctgataccg cttcagaagg ctactgatgt
     9181 ctacgcttct ttatttacac ggtttcaaca gctcgccgcg ctctgcaaaa gcgagcttgt
     9241 taaaaaactg gctggcggaa catcaccctg acgttgagat gatcattccg cagttgccgc
     9301 cgtatccttc cgacgcggca gaactgctgg aatccattgt cctggaacat ggcggcgatt
     9361 cgctgggtat tgtcggttcg tcactggggg gatattacgc cacctggttg tcacaatgtt
     9421 ttatgctgcc cgcagtggtg gtaaacccgg cggtgcgccc gtttgaactg ctgacggact
     9481 atctcggtca gaacgagaac ccatacaccg ggcagcaata tgtgctagag tcacgccata
     9541 tttacgatct caaagtcatg cagattgacc cgctggaagc gccggatttg atctggctgc
     9601 tgcaacagac gggagatgaa gtgctggatt accgccaggc ggtggcgtac tacgcttcct
     9661 gccgccagac tgtcatagaa ggcggcaacc acgcattcac gggcttcgaa gattatttca
     9721 acccgatcgt cgattttctt ggtctgcacc atctctgacg atcaactcga attgctaact
     9781 taaaccatga cgcaaactta taacgctgat gccattgagg tactcaccgg gcttgagccg
     9841 gttcgccgcc gtccggggat gtataccgat accactcgcc ctaaccattt ggggcaagaa
     9901 gttattgata acagtgtcga tgaagcactg gcgggtcacg caaaacgcgt ggatgtaatc
     9961 ttacatgctg accagtcgtt agaagttatt gacgatgggc gcgggatgcc ggtggatatt
    10021 cacccggaag agggtgtccc ggcggttgaa ttgattcttt gccgtctgca tgcaggcggt
    10081 aaattctcta acaaaaatta ccagttctct ggcggcctgc atggcgtggg gatttcggtg
    10141 gttaacgccc tgtcgaagcg cgttgaagtt aacgtgcgcc gcgatggtca ggtttataac
    10201 atcgcctttg aaaatggcga aaaggtgcag gatttacagg ttgtcggcac ttgcggtaaa
    10261 cgcaataccg gtaccagcgt gcacttctgg ccggatgaaa ccttctttga cagcccgcga
    10321 ttttctgttt cacgcctgac gcatgtgctg aaagccaaag cggtactgtg ccccggcgtt
    10381 gagattacct ttaaagatga gatcaacaac accgaacagc gctggtgcta tcaggacggt
    10441 ctgaatgatt acctggcgga agcggtaaac ggtttaccga cgctgccaga aaaaccgttt
    10501 atcggtaatt tcgctggcga tactgaagct gtggactggg cgctactgtg gctgccggaa
    10561 ggcggtgaac tgctgaccga aagctacgtc aacctgatcc caacgatgca gggcggtacc
    10621 catgttaatg gcctgcgtca gggcctgttg gacgcgatgc gtgagttctg tgaatatcgc
    10681 aacattctgc cgcgcggcgt gaagctgtcg gcggaagata tctgggatcg ctgcgcctat
    10741 gttctgtcgg tgaaaatgca ggatccgcag tttgccgggc agacgaaaga gcgtctctct
    10801 tcgcgtcaat gcgcggcatt cgtttctggc gtggtgaaag atgcctttat cctgtggctg
    10861 aaccagaacg ttcaggcggc ggagttgctg gcagagatgg cgatttccag cgcccagcgt
    10921 cgtatgcgtg cggctaaaaa agtggtgcgt aaaaagctga ccagcggccc ggcgctgcct
    10981 ggcaaactgg ctgattgtac cgcgcaggac cttaaccgta ccgaactgtt cctcgtggaa
    11041 ggtgactccg caggcggatc tgccaagcag gcgcgcgatc gtgaatatca ggcgatcatg
    11101 ccgctgaaag gtaagatcct taacacctgg gaagtttctt ccgacgaagt gctggcctcg
    11161 caggaagtgc atgatatttc ggtagcgatc ggtatcgatc ctgacagcga cgatctgagc
    11221 cagcttcgtt atggcaaaat ctgtatcctc gcggatgcgg actctgatgg tctgcacatt
    11281 gccacgctgc tctgcgcttt gttcgtaaaa cacttccgcg cgttggtgaa acacggtcac
    11341 gtttacgtcg cactgccacc gctctaccgt attgatctcg ggaaagaggt ttattacgcg
    11401 ctgacggaag aagagaaaga gggcgtactt gagcaattaa aacgcaagaa aggcaagccg
    11461 aacgtccagc gttttaaagg tctgggggaa atgaacccga tgcaattgcg tgaaaccacg
    11521 cttgatccga acactcgccg tctggtgcag ttgactatcg atgatgaaga cgatcagcgt
    11581 actgacgcga tgatggatat gctgctggcg aagaaacgct cggaagatcg ccgcaactgg
    11641 ttgcaagaga aaggcgacat ggcggagatt gaggtttaaa ggaaagaaca ttgcccggtc
    11701 aggatggccg ggcaaacaag gcaggattaa atccctggct gcagaatacg aatattcatc
    11761 tccagcacgt cgccttttac ggcttcgctg tacgccttca tgtgcggggt ttgcagatgc
    11821 gcttcaaggt gcgcgatgct ttcccactgc tcaatcatca cgatagaatc cggtgccata
    11881 gactggaaac tcacgccagc agcgcaatcc accattggcg catagccgtg gcaaccttct
    11941 tctttcagta cggttggaac gattttagca aactgatcca ataccgcctg acggtgatgt
    12001 tgaccaggac gagtacggat ttctgcgatt acggtaagca tggttaactc cttctaaagc
    12061 ctgagctcta gttaaccaaa aatttccaca agatgcttgc gatattcttc agtatagcgg
    12121 ggaacatcag gcatttttat cacgtcatta gcgataaatg tcggcagcgg ttccatgcca
    12181 aggaattggt ttgctttatg gaacggcaga tacacaccgt cgacgccaac gccgtggaag
    12241 aattgatctt tttcggtgaa ggcttccatt ggtgcgttcc aggtcagaga aagcatatat
    12301 tttttgccct gtaccaggcc gccggaaccg tatttttttg acggatcttt gcgggtacgg
    12361 ccatcgctgg catacagcgt cccgtgacct tcggtgaata catcatcaat gtattttttc
    12421 actgtccacg gcgcgcccat ccaccagcct ggcatctgcc agatcaccac atcagcccag
    12481 agaaagtttt gtacttccgc tttgacatcg tagtcgctgt cggcgcgaac gatgcgaaca
    12541 tcatgcccga ggtcgcgcag tgtgccatcc gcgacttcgg tcagggtgtc gttcagttga
    12601 ccattggagt gggcgaattt tttcgcgccg ttgataatca gggtgttgct cattttttaa
    12661 cctcaaggtg agacgtttgt cgactatagt acgtcacaga gcggtacggt aaaattagca
    12721 aaatgtgcaa agtcttttgc gaatttagca aaagtcttac ccttaccagc tcaccttcgc
    12781 ctcaaatcca ccttgttccg cattcccaaa ttcaacattc atgccatgca atttggcgat
    12841 tcgctggaca atcgatagcc caagtccact gccggtagcg gtttgtccgg gggggcgata
    12901 gaagcgttcg ccaattcgcg ccagtgcctc tggtgtcaca ccggggccgt tatccctcac
    12961 gatgaaatta tcggcattca gcgtgacgtc caccacgctg ccctgtggac tgtagcgcac
    13021 ggcgttatcc agaaggtttc gcaccaacaa acttagcaat agcggttgtc cagtgcgttt
    13081 gatgctgtgg gcattgagtg tcagtcgcac gtcaattttc gcctgctgcg ccgtgtggta
    13141 aatatccatc accgacgatt gcaggagatc ttcaagcggg atctccgcga cgtcctgaag
    13201 gttatccagt gagtccagcc gcgatagcgt gagcagttga tcaaccagac gagtagcgcg
    13261 atcgatcccg gaatgtaatt ggagcagtgc ttttttccgc gcctgcggat catcgtcaga
    13321 gagctgcgca acttcggttt gcactttcag cgctgttaac gggctacgta gttcgtgagc
    13381 tgcatcggag gtaaagcgtc gttctcgaac catcatcgca tgtgtgcggg cgaacagttg
    13441 atttagcgac tcaaccagtg gtcgcacttc gctgggtacg ccagtcgcgt ttagtggttt
    13501 ttccgagtca gggtcacgca tacgtagtgc cagcgccagt ttgttcagcg gcgcgagttc
    13561 acgacccagt agtaccatca tgatgattaa cataatcggc agtgcgacca gccacgggat
    13621 caattgcccg gcaacaatcg ccagcgccat gtcttcacgg tattcccatt cctggccaac
    13681 aacgatgcga tatttgccat caggtgaggt catccagaca aaacgccaag gatcgtcttc
    13741 accgaccagt tgcccgtcag caaaaccttc ccgttgatag ctatagggaa tatcttctcc
    13801 gttatcgcca tcattaagga ccattctgcc gtcgtgggta aagatggcaa aggtcagcgc
    13861 atcgtcatca acatgaccgt gttttaattt atttggcgtc tgtgccatgc gatccgccgc
    13921 gttgatttcg ttgagatcga gcgtacttaa ccgcttggca aacagcatca gttgggtgtc
    13981 gaacaattca tcgacgttat ccgttgtttg tttccaggcg acaaagctgg aaagcagcca
    14041 ggtcaccgag gccagaatta aaaagattag cgtcagcctg acgcgcagac taagacgttg
    14101 ggtaaatttc atttctcacc taatgtgtag ccaataccat gcacggtacg aataaaatca
    14161 ctaccgagtt tgcgtcgcag atgatgcaca tgcacttcaa cggcattact ggtgacctct
    14221 tcgtcccagg tatacagttt ctcttcaatc agtttgcgcg gcagtacccg accagcgtta
    14281 cgcatcagta attccagcag ggcaaattct tttggtttca gtgttaaggg ttcgccagcc
    14341 agcgtggcga tacgtttgcc ggggtcgagc atgacgttac cgtggcgcag ctcgttgctg
    14401 gcctggccgt tggttcggcg catcagagcc tccagcctgg cggcgacttc tatcaacgca
    14461 aaaggtttac acagataatc gtcagctccc agacgcagcc cttctacacg ttccgctaac
    14521 gcatcacgcg cggtcaggat cagtaccggc tcacgctgac ctttctctcg ccattcgcgc
    14581 aaaatatcgc gaccatccat gcctggtaag gttaaatcca ggatcaccgc atcataaggt
    14641 gcgctataaa gcgcctcttt tccctgacga ccttgtgtaa accagtcgac actaaaaccc
    14701 attttactaa ggccagtttt gatgccgtcg ccaatcagca tgtcatcttc tatcagtaaa
    14761 attcgcattt tttcatccct gcgataaccg tatttgctgc cagtaaaccg tgttgtaacg
    14821 cgctctgtac agcattaaat tttatttttt tcccttaaga agttgttaag gactatcttg
    14881 ttaaatgctc ggaaacagac attaaaggga gtaataaaca tgaaaaaatt cgcagcagta
    14941 atcgcagtaa tggccctgtg cagtgcaccg gtgatggcag cagagcaggg cggtttttct
    15001 ggcccatcgg caacgcaaag tcaggccgga ggattccagg ggccgaacgg cagcgtaacg
    15061 actgtagaaa gcgcaaaatc cctgcgtgac gacacctggg taaccctgcg cggcaatatc
    15121 gttgaacgca tctctgacga tctctacgtg tttaaagatg ccagcggtac tatcaatgtt
    15181 gatatcgacc acaaacgctg gaacggtgtg acggtgacgc cgaaagatac ggttgagatt
    15241 cagggtgaag tcgataaaga ctggaattct gttgaaattg acgtcaaaca gatccgcaaa
    15301 gtaaatccgt aattgttacc gctcccggga cgcgttcccg ggaataattt cgcagggagg
    15361 caaaatgaca aacctgacac tggatgtaaa cattatcgat ttcccatcaa tccctgtggc
    15421 gatgttgccg caccgctgta gccctgagtt gctcaactac agtgtggcga aatttatcat
    15481 gtggcgtaaa gagacggggc tttctcctgt taaccaaagc cagacttttg gcgtcgcctg
    15541 ggacgaccct gccaccaccg caccggaagc gtttcgcttt gatatctgcg gcagcgttag
    15601 cgaaccgatt cccgataatc gttatggtgt gagcaatggt gaacttaccg gtggacgtta
    15661 tgccgtggcc cgccacgttg gcgagctgga cgatatttca cacacgatat ggggcattat
    15721 tcgccactgg ctgcctgcaa gcggcgagaa aatgcgtaaa gcaccgattc tgtttcacta
    15781 caccaatctt gccgaagggg tgacggagca gcgactggaa acggatgttt atgtgccgtt
    15841 agcgtgatgg ggaatcttta ccggagtgtg agccaaatca ctcgcgataa gtttgagcga
    15901 acaatgccac aggcgtattg ttgttgggct tataatttct ccggtaagga ataccatgta
    15961 tacgcgaaat ttattatccg gtgcgccgta agccgtctcg ggggtaccca tgagcaagcc
    16021 gcacttatgg caggaggcgc ggggcactgg tggagacaca gctaaactgg gctgaaccat
    16081 atacttacag tcgactactt tgatgcttta ggcttccctg gactcgcata acctcaactt
    16141 ctcgaatcgc ccggagcgga cccccatgcc gagtggtgtg gcatgggcgc aaccttgtgc
    16201 tgccctctat gccgatcaag tctacagcaa agagttgtat atcaaggcac attgatgcct
    16261 ttatcgccgt cggagcacgt ccggcggcgc tattcacgct gatctcctgt gactcgacgc
    16321 ggcagataat gtagtatctc cggcaatatt gcccctttga aggctggcga atcagttgag
    16381 gaatcagaat taatgagcga tatggcagag cgccttgcgc tacatgaatt tacggaaaac
    16441 gcctacttaa actactccat gtacgtgatc atggaccgtg cgttgccgtt tattggtgat
    16501 ggtctgaaac ctgttcagcg ccgcattgtg tatgcgatgt ctgaactggg cctgaatgcc
    16561 agcgccaaat ttaaaaaatc ggcccgtacc gtcggtgacg tactgggtaa ataccatccg
    16621 cacggcgata gcgcctgtta tgaagcgatg gtcctgatgg cgcagccgtt ctcttaccgt
    16681 tatccgctgg ttgatggtca ggggaactgg ggcgcgccgg acgatccgaa atcgttcgcg
    16741 gcaatgcgtt acaccgaatc ccggttgtcg aaatattccg agctgctatt gagcgagctg
    16801 gggcagggga cggctgactg ggtgccaaac ttcgacggca ctttgcagga gccgaaaatg
    16861 ctgcctgctc gtctgccaaa cattttgctt aacggcacca ccggtattgc cgtcggcatg
    16921 gcgaccgata ttccaccgca taacctgcgt gaagtagctc aggcggcaat cgcattaatc
    16981 gaccagccga aaaccacgct cgatcagctg ctggatatcg tgcaggggcc ggattatccg
    17041 actgaagcgg aaattatcac ttcgcgcgcc gagatccgta aaatctacga gaacggacgt
    17101 ggttcagtgc gtatgcgcgc ggtgtggaag aaagaagatg gcgcggtggt tatcagcgca
    17161 ttaccgcatc aggtttcagg tgcgcgtgta ctggagcaaa ttgctgcgca aatgcgcaac
    17221 aaaaagctgc cgatggttga cgatctgcgc gatgaatctg accacgagaa cccgacccgt
    17281 ctggtgattg tgccgcgttc caaccgcgtg gatatggatc aggtgatgaa ccacctcttc
    17341 gctaccaccg atctggaaaa gagctatcgc attaacctca atatgatcgg tctggatggt
    17401 cgtccggcgg tgaaaaacct gctggaaatc ctctccgaat ggctggtgtt ccgtcgcgat
    17461 accgtgcgcc gccgactgaa ctatcgtctg gagaaagtcc tcaagcgcct gcatatcctg
    17521 gaaggtttgc tggtggcgtt tctcaacatc gacgaagtga ttgagatcat tcgtaacgaa
    17581 gatgaaccga aaccggcgct gatgtcgcgg tttggcctta cggaaaccca ggcggaagcg
    17641 atcctcgaac tgaaactgcg tcatcttgcc aaactggaag agatgaagat tcgcggtgag
    17701 cagagtgagc tggaaaaaga gcgcgaccag ttgcagggca ttttggcttc cgagcgtaaa
    17761 atgaataacc tgctgaagaa agaactgcag gcagacgcgc aagcctacgg tgacgatcgt
    17821 cgttcgccgt tgcaggaacg cgaagaagcg aaagcgatga gcgagcacga catgctgccg
    17881 tctgaacctg tcaccattgt gctgtcgcag atgggctggg tacgcagcgc taaaggccat
    17941 gatatcgacg cgccgggcct gaattataaa gcgggtgata gcttcaaagc ggcggtgaaa
    18001 ggtaagagca accaatcggt agtgtttgtt gattccaccg gtcgtagcta tgccatcgac
    18061 ccgattacgc tgccgtcggc gcgtggtcag ggcgaaccac tcaccggcaa attaacgttg
    18121 ccgcctgggg cgaccgttga ccatatgctg atggaaagcg acgatcagaa actgctgatg
    18181 gcttccgatg cgggttacgg tttcgtctgc acctttaacg atctggtggc gcgtaaccgt
    18241 gcaggtaagg ctttgatcac cttaccggaa aatgcccatg ttatgccgcc ggtggtgatt
    18301 gaagatgctt ccgatatgct gctggcaatc actcaggcag gccgtatgtt gatgttcccg
    18361 gtaagcgatc tgccgcagct gtcgaagggc aaaggcaaca agattatcaa cattccatcg
    18421 gcagaagccg cgcgtggaga agatggtctg gcgcaattgt acgttctgcc gccgcaaagc
    18481 acgctgacca ttcatgttgg gaaacgcaaa attaaactgc gcccggaaga gttacagaaa
    18541 gtcactggtg aacgtggacg ccgcggtacg ttgatgcgcg gtttgcagcg tatcgatcgt
    18601 gttgagatcg actctcctcg ccgtgccagc aacggtgata gcgaagagta atatgccggg
    18661 gagggcctgt ttcctcccgc ttgcaaggaa cgccggatga aacgttaagt cgtcatccgg
    18721 cagcgtattc aacccccgga aacgataaaa attcccttga aaccgttgtt tattcatgcg
    18781 ttgcgattaa caatacgctt ttccagagag cggcttttaa caatgcccta acctgatttc
    18841 aggtgacgta caatgccagt ctctcagacc ttcagagggt gttatgctat atatctttcg
    18901 tcttattatt accgtgattt acagcatctt agtctgtgta ttcggctcca tttactgcct
    18961 tttcagcccg cgtaacccga aacatgtggc cacctttggg catatgtttg gccgtcttgc
    19021 gccgctgttc ggcctgaaag ttgagtgccg caaacctgca gatgctgaaa gctacggcaa
    19081 tgctatctat atcgctaacc accagaacaa ctatgacatg gtgacagcat cgaacattgt
    19141 gcaaccgccg acggtgacgg taggtaaaaa gagcttgctg tggatcccct tcttcgggca
    19201 gttgtactgg ttaaccggca acttattgat cgacagaaac aatcgcacta aagctcacgg
    19261 caccattgcg gaagtcgtga atcatttcaa aaaacgccgt atttccatct ggatgttccc
    19321 ggaaggaacc cgcagccgtg gtcgcggcct gctaccgttc aagactggag catttcacgc
    19381 ggcaattgcg gcgggcgtcc cgattattcc cgtgtgcgtc tctacaactt cgaataagat
    19441 taatcttaat cgactgcaca acggtctggt gattgtcgaa atgctgccgc caattgacgt
    19501 cagtcagtat ggcaaagatc aggttcgtga gctggctgcc cattgtcgtt cgataatgga
    19561 acaaaaaatc gccgagctcg ataaagaagt cgcagaacgc gaagccgccg gaaaagttta
    19621 agtcggcaat ctgtattttt gcggggaaca ctttcctgca cggtattact ttagccagtt
    19681 ttacatggag caaatatgtc actcagtcgg cgtcagttca ttcaggcatc ggggattgca
    19741 ctttgtgcag gcgctgttcc cctgaaggcc agcgcagccg ggcaacagca accgctaccc
    19801 gttccgccgc tacttgaatc tcgccgtggg caaccgctgt ttatgactgt acaacgtgcg
    19861 cactggtcat ttacgccagg gacacgcgcg tcggtctggg gaatcaatgg tcgttacctg
    19921 gggccgacta tccgcgtctg gaagggcgac gatgttaagc ttatttacag caaccgcctg
    19981 acagaaaatg tctcaatgac ggtggccggg ctacaggtac ctggcccgct gatgggcggt
    20041 ccggcacgga tgatgtcgcc aaacgctgac tgggcacccg tactgcctat tcgccagaac
    20101 gcagctactc tgtggtatca cgccaatact cccaaccgca cggctcagca ggtctataac
    20161 ggccttgccg gaatgtggct ggtggaagat gaagtcagca agtcgctgcc tatccccaac
    20221 cattatgatg tggatgattt tccggtcatt atccaggata aacggctgga taactttggt
    20281 acgccagaat acaacgaacc gggaagcggc ggctttgttg gtgatacgct gctggttaac
    20341 ggtgtacaaa gcccgtacgt tgaagtctcg cgtggctggg tgcgcttgcg actgctgaac
    20401 gcgtcgaact ctcgtcgcta tcaactacag atgagcgatg gtcgcccgtt acatgtgatt
    20461 tctggcgatc agggattcct gcctgctcct gtatcggtga agcaactttc gctggcaccg
    20521 ggcgagcgcc gcgagattct ggtggatatg agcaacggcg atgaagtgtc gatcacctgt
    20581 ggcgaagcgg cgagcattgt tgatcgtatt cgcggcttct ttgagccatc cagtattctg
    20641 gtttctaccc tggtgctaac gctgcgccca accggccttc tgccgctggt cacagacagt
    20701 cttccgatgc gcttgctgcc aactgaaatc atggccggtt cgccgattcg cagtcgcgat
    20761 atcagtctgg gtgatgaccc gggtattaat ggacagctgt gggacgtcaa ccgtattgat
    20821 gtcaccgcgc agcaaggaac gtgggaacgc tggacggtac gcgcggacga gccgcaagcg
    20881 ttccatattg aaggcgtgat gttccagatc cgtaacgtga atggtgcgat gccgttcccg
    20941 gaagacagag gctggaaaga taccgtttgg gttgacggac aagtggagct gcttgtttat
    21001 ttcggtcagc cttcctgggc gcacttcccg ttctacttca acagtcagac gctggaaatg
    21061 gcggaccgtg gctcgattgg gcaactgtta gtcaatccgg taccgtaatc ttcatactcg
    21121 cccgaggagg gcgagtatgg aatacttttc gatttctctt tcgttccggc ttataatccc
    21181 cggcctttga ttattttttt atcatttctg gaagcaaaat gagctctatc tccctgatcc
    21241 aaccggatcg cgacctgttc tcctggccgc agtactgggc cgcctgtttt ggaccggcac
    21301 cgtttttgcc gatgtctcgt gaagagatgg atcaacttgg ctgggatagc tgcgacatca
    21361 ttttggttac tggcgacgcg tatgtcgatc acccaagctt cgggatggcg atttgcggtc
    21421 gtatgctgga agcacagggc tttcgcgtcg ggatcatcgc ccagccggac tggagcagca
    21481 aagacgactt tatgcgtctg ggtaaaccga atctgttttt cggtgttact gctggcaaca
    21541 tggattcgat gatcaaccgt tataccgccg atcgccgttt acgtcatgac gatgcctaca
    21601 cgccggataa cgtcgcgggt aagcgcccgg atcgcgccac actggtttat acccagcgtt
    21661 gtaaagaggc gtggaaagat gtaccggtga tcctcggcgg tattgaggct agtctgcgcc
    21721 gtaccgcaca ttatgattac tggtccgata ccgtgcgccg ttccgtgctg gtggattcga
    21781 aagccgacat gctgatgttt ggtaacggtg agcgtccgct ggtggaggtg gcgcaccgtc
    21841 tggcgatggg cgagccgatt agtgaaatcc gcgatgtgcg taataccgcg attatcgtga
    21901 aagaggcgct gcctggctgg agcggcgtgg attccacccg tcttgatacc cctggaaaaa
    21961 tcgacccaat cccgcatccg tatggtgaag atttgccgtg cgcggataac aaaccggtgg
    22021 caccgaaaaa gcaggaagcc aaagccgtaa ccgtgcagcc accgcgcccg aaaccgtggg
    22081 aaaaaaccta cgtgttgctg ccttctttcg agaaagtgaa gggcgataaa gtgctgtacg
    22141 cccatgcttc gcgtattctg caccacgaaa ccaacccagg ctgcgtccgc gcattgatgc
    22201 aaaaacacgg tgaccgctat gtgtggatca acccgcctgc tatcccgctt tctaccgaag
    22261 agatggacag cgtttttgcg ctgccgtaca agcgcgtgcc acatccggcc tatggcaatg
    22321 cccgtattcc ggcttacgaa atgatccgtt tttcggtcaa cattatgcgc ggctgctttg
    22381 gcggctgttc tttctgttct attaccgagc acgaagggcg cattattcag agccgttcgg
    22441 aagattcgat cattaatgag atcgaagcga tccgcgacac cgttccaggt tttacgggcg
    22501 tgatttccga tctcggtggg ccaactgcca acatgtatat gttgcgctgc aaatcgccac
    22561 gcgctgaaca aacttgtcgc cgtttgtcgt gcgtttatcc ggatatttgt ccgcacatgg
    22621 acactaacca cgaaccgacg atcaacctct atcgccgtgc gcgtgatctg aaaggcatta
    22681 aaaaaatcct catcgcctca ggcgtacgtt atgacatcgc cgtggaagat ccgcgctata
    22741 tcaaagagtt ggcgacccat cacgtcggcg gttatctgaa gattgctccg gaacataccg
    22801 aagaagggcc gttgtcgaag atgatgaagc cgggcatggg cagctatgac cgctttaaag
    22861 agctgttcga tacctactcg aaacaggcag gtaaagagca gtatctgatc ccgtatttca
    22921 tctctgcgca ccccggtacg cgtgatgaag atatggtgaa tttggcgctg tggctgaaaa
    22981 agcatcgttt ccgtctcgac caggtacaga acttctaccc atcgccgctg gcgaactcaa
    23041 ccaccatgta ttacaccggg aaaaaaccgc tggcgaagat tggttataag agcgaagacg
    23101 tcttcgtacc gaagggcgac aaacagcgtc gtttgcataa agcgttgttg cgttaccacg
    23161 atccggcaaa ctggccgtta atccgcccgg cgctggaagc gatgggcaaa aagcatctga
    23221 ttggcagccg tcgcgattgc ttagtgcctg cgccaaccat tgaagagatg cgtgaagctc
    23281 gtcgccagaa ccgcaatacc cgtccggcgt tgactaaaca tacgccgatg gcgacccagc
    23341 gtcagacgcc tgctacggca aaaaaagcgt cgtctacgca atctcgtccg gtgaatgctg
    23401 gtgcgaagaa acgccctaaa gcggcggttg gacgttaaaa aaaatccccg gcaactgaca
    23461 tgctgccggg gattttttta tcattctgcg tacatacgca agcagcgcag gcaatcaaaa
    23521 tagtcctgag catcaagacg cgtattggct ctgttagctt cggatttggc agcatagatt
    23581 tgtgggcgta gtgcttttat atcctgctca agccgggcga ttttggcatt taatgtttgt
    23641 acattgctcg ccagttgatt gaccttttgc tcattgacgc agccactgag caagcaggtt
    23701 gccagaacaa cagctcccac ggtgaaaatc gttttcatat agtgttccct tttatttatt
    23761 gttccggcta attgttattt aattcagctc attgctttaa tctggcggcg gtttttgcct
    23821 caatctgctt aagtaggctg ccgctgttcg ggcggataaa atcagcgatt tcccacttgc
    23881 cttcgcgccg ctgtaaaacc agttcttgct cttcgttgtt gccattggtc agcgtaaaac
    23941 gaacatgaat gcgggcggaa tcggcggttt tctccaggac tcgaatttca tcaacgcgtg
    24001 aaaatagcga ctcttcgcca aaacagatcg gattgtcgta aataaattgc cgttctattt
    24061 gcatatcttc atcgggatta ttggccgtct tgaaggtggt tttatagaag gcattaaccg
    24121 cctggcggta agagtcggaa accacccacg tccacggcca gctataatca gcaatatgtt
    24181 caaagagttc ggcaacaaag gcttccggct gttctttttg caacgaagct aaagcggcca
    24241 gcgttttttc attctcgcta ttaactgctt gtaagacgct gccatgattg ctgacaatat
    24301 cgtcaatgac ccaacgacca ttttccgcca ccattttcag tgtctgcgtg gtcttttctt
    24361 tatcatcttt aaagatacga aagcgcacaa cggcatcggc atgatcggcg tcagtttggg
    24421 ttatcgcaac gctttctagc accagatcgc caaaatcctg acaatcacaa accggatcat
    24481 aatccagcca gccaatattg cccggcagcg taagattgtc gttcagggta agcgcctgtt
    24541 gaatacgcgc tgaagttatc gcccgctctc cggtttcacc gaaataaggt gtactggcat
    24601 ctgatttata gttctgataa atctgccgga ctgtttgttc tacggtctgt gaaggaggct
    24661 gcgcgtgtac ggtaaaactt gccagggctg ctaaaaacag aagtattatt ttcatctgtt
    24721 tttcacctct ccgttgcgtt tatcccggat atagatgagt tgttctttta agagcttccg
    24781 gct
//
LOCUS       DAZOZY010000045        24386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZOZY010000045 DAZOZY010000000
VERSION     DAZOZY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.045"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            271..408
                     /locus_tag="ACHURQ_001571"
                     /pseudo
     CDS             271..408
                     /locus_tag="ACHURQ_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(516..1751)
                     /locus_tag="ACHURQ_001572"
     CDS             complement(516..1751)
                     /locus_tag="ACHURQ_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0823383.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1898..1974)
                     /locus_tag="ACHURQ_001573"
     tRNA            complement(1898..1974)
                     /locus_tag="ACHURQ_001573"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Pro,seq:tgg)
     gene            complement(2017..2103)
                     /locus_tag="ACHURQ_001574"
     tRNA            complement(2017..2103)
                     /locus_tag="ACHURQ_001574"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2067..2069),aa:Leu,seq:cag)
     gene            complement(2124..2199)
                     /locus_tag="ACHURQ_001575"
     tRNA            complement(2124..2199)
                     /locus_tag="ACHURQ_001575"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2164..2166),aa:His,seq:gtg)
     gene            complement(2258..2334)
                     /locus_tag="ACHURQ_001576"
     tRNA            complement(2258..2334)
                     /locus_tag="ACHURQ_001576"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2298..2300),aa:Arg,seq:ccg)
     gene            complement(2437..3822)
                     /locus_tag="ACHURQ_001577"
     CDS             complement(2437..3822)
                     /locus_tag="ACHURQ_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0823384.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(4013..4753)
                     /gene="wecG"
                     /locus_tag="ACHURQ_001578"
     CDS             complement(4013..4753)
                     /gene="wecG"
                     /locus_tag="ACHURQ_001578"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR0823385.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4756..6108)
                     /gene="wzyE"
                     /locus_tag="ACHURQ_001579"
     CDS             complement(4756..6108)
                     /gene="wzyE"
                     /locus_tag="ACHURQ_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR0823386.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6105..7184)
                     /gene="rffT"
                     /locus_tag="ACHURQ_001580"
                     /gene_synonym="wecF"
     CDS             complement(6105..7184)
                     /gene="rffT"
                     /locus_tag="ACHURQ_001580"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR0823387.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7181..8431)
                     /gene="wzxE"
                     /locus_tag="ACHURQ_001581"
     CDS             complement(7181..8431)
                     /gene="wzxE"
                     /locus_tag="ACHURQ_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR0823388.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8433..9563)
                     /gene="rffA"
                     /locus_tag="ACHURQ_001582"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8433..9563)
                     /gene="rffA"
                     /locus_tag="ACHURQ_001582"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR0823389.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9568..10242)
                     /gene="rffC"
                     /locus_tag="ACHURQ_001583"
                     /gene_synonym="wecD"
     CDS             complement(9568..10242)
                     /gene="rffC"
                     /locus_tag="ACHURQ_001583"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR0823390.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10220..11101)
                     /gene="rfbA"
                     /locus_tag="ACHURQ_001584"
     CDS             complement(10220..11101)
                     /gene="rfbA"
                     /locus_tag="ACHURQ_001584"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR0823391.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11120..12187)
                     /gene="rffG"
                     /locus_tag="ACHURQ_001585"
     CDS             complement(11120..12187)
                     /gene="rffG"
                     /locus_tag="ACHURQ_001585"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR0823392.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12187..13449)
                     /gene="wecC"
                     /locus_tag="ACHURQ_001586"
     CDS             complement(12187..13449)
                     /gene="wecC"
                     /locus_tag="ACHURQ_001586"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR0823393.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13446..14576)
                     /gene="wecB"
                     /locus_tag="ACHURQ_001587"
     CDS             complement(13446..14576)
                     /gene="wecB"
                     /locus_tag="ACHURQ_001587"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR0823394.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14632..15678)
                     /gene="wzzE"
                     /locus_tag="ACHURQ_001588"
     CDS             complement(14632..15678)
                     /gene="wzzE"
                     /locus_tag="ACHURQ_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR0823395.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15690..16793)
                     /gene="wecA"
                     /locus_tag="ACHURQ_001589"
     CDS             complement(15690..16793)
                     /gene="wecA"
                     /locus_tag="ACHURQ_001589"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR0823396.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17033..18292)
                     /gene="rho"
                     /locus_tag="ACHURQ_001590"
     CDS             complement(17033..18292)
                     /gene="rho"
                     /locus_tag="ACHURQ_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR0823397.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18377..18478)
                     /gene="rhoL"
                     /locus_tag="ACHURQ_001591"
     CDS             complement(18377..18478)
                     /gene="rhoL"
                     /locus_tag="ACHURQ_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR0823398.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18619..18948)
                     /gene="trxA"
                     /locus_tag="ACHURQ_001592"
     CDS             complement(18619..18948)
                     /gene="trxA"
                     /locus_tag="ACHURQ_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR0823399.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19079..20344
                     /gene="rhlB"
                     /locus_tag="ACHURQ_001593"
     CDS             19079..20344
                     /gene="rhlB"
                     /locus_tag="ACHURQ_001593"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR0823400.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20478..21962
                     /gene="gppA"
                     /locus_tag="ACHURQ_001594"
     CDS             20478..21962
                     /gene="gppA"
                     /locus_tag="ACHURQ_001594"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR0823401.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(22009..24030)
                     /gene="rep"
                     /locus_tag="ACHURQ_001595"
     CDS             complement(22009..24030)
                     /gene="rep"
                     /locus_tag="ACHURQ_001595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR0823402.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24247..>24386
                     /locus_tag="ACHURQ_001596"
     CDS             24247..>24386
                     /locus_tag="ACHURQ_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823403.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6144 a         6672 c         6010 g         5560 t
ORIGIN      
        1 ttcattcaat taataacaca tatattaatt gccgttaaaa ctaaaaaagc attaataatc
       61 aacgcaatat aataaacccg ccttacatat caactgcgcc agaggtagga ttgaaaacgc
      121 tctcctgatt ttccaattca ttttctggat aaataaataa tttatttttg tcactattat
      181 ttatgtaatc atcctgtcag ggagagggat ctcaattatc aatgcttaat tacgtcatca
      241 ttttgatgac atgaaaccgg agcattttgc atggaatttt cgttttcacc caaacttctt
      301 gttgttgctg tcgccgccgc tcttcctctc atggccagcg cagcagatac cccgtcaact
      361 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
      421 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      481 aatcaaccgc aggcggccac aaaatggccg cttttttact tattcaccac cagcaaatgt
      541 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      601 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      661 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccagca cgcttttaac
      721 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      781 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      841 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      901 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      961 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcgaat
     1021 atcgccacca gaaattcacc aaattcatcg gggcgcaccg accattccgt cagttccccc
     1081 tgaatatcac caggcgcatg caatttcagt ccatcgcggg cagctgtttc atcagccaga
     1141 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
     1201 acctgcaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
     1261 tgatgttttt gcagcagcgt cagggcacgc atcaccagct tatgcgttgg tctgccacct
     1321 ttggtcacgc gatattgatt gtggatctca gccggaccgt ccagcgataa cccaacaaga
     1381 aaatgatttt ctgccagaaa tgcacaccat ttatcatcga gcagcacgcc gttagtctgg
     1441 aagctgttac ttatcttcct gccagcaccg tatttcgcct gtaactttac ggcacggcgg
     1501 taaaaatcca gcccgagtaa cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1561 tgcggttcgc tggcagcgat atagtgacgg acatacgctt ccagcgtgtc atcgtccata
     1621 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1681 ttcagattac aatcggaacc actcggtttc gccatcacat gaaaagcacg cgttggaacc
     1741 tgttgcagca tggtcactcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1801 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1861 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1921 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1981 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     2041 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     2101 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     2161 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     2221 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     2281 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     2341 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2401 attgcgcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2461 cccgtggcga ttaaggccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2521 gccaacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2581 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2641 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2701 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2761 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2821 cgcaactggt acgccgtgac gggaaacttt cgccatcgcc gccggtaact gacggttttt
     2881 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2941 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     3001 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     3061 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacagcact
     3121 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     3181 ctggtaggac gccaccacaa tacacagcgc ggtcaggaac cctttccagc cacccgcaaa
     3241 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     3301 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3361 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3421 cagcgccacc gcgatcaatg caggtatcca ctgcgccatc tccgggaacc agaactggac
     3481 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3541 ccaggcggtg agataaccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3601 accggtaaca ggttcaagga acaacatttc gcccattgaa cgcatgatga aaaagacgaa
     3661 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3721 ggcccccata aacaggccga cgccaatagt gccccccagg gcgatgagtt cgatatgtcg
     3781 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3841 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3901 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3961 aagcagacag gcgacggagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     4021 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     4081 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     4141 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     4201 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     4261 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     4321 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4381 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4441 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4501 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4561 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcagcat
     4621 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcgt
     4681 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4741 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4801 aatgagtccg gcgctttcaa aaagccagta cagcagtttt gcgattatca gacatgcgcc
     4861 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgcgccag
     4921 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4981 attaggctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagaccaac
     5041 cacgatcgcc ccgagcggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     5101 cgtaggcgag atcgccagtc cggagtggtt attcagcact tcccaggtaa agtagttggc
     5161 tgagttcagc accatactcg ggcgacccgg ccacagccag gaagggataa agacatagaa
     5221 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     5281 cgccagattc tcccacgggg agaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     5341 ttcatcgccg ctcacattca ttccatagcg ttttagcgcc atccagaaca tgccaacaat
     5401 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5461 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5521 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5581 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5641 cacgccggag acttcactgg agaagatctg actgtaggag ttaagccgga acagcaaaaa
     5701 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5761 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5821 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5881 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5941 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     6001 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     6061 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     6121 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     6181 gtgtttttat ccaccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     6241 tcgtcggtag taaacagcac cggcaaatgc tgttccgtca tatcctgcca gaacggattt
     6301 tcccggttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6361 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6421 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6481 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6541 acttttaccg tatcgccaaa ttgctgatga acggcgcgca aggcagcaat atggtcattg
     6601 ctgcggtcac cggagttccc caccagaatg gtcattttcc cttcacgttg ccgatcattc
     6661 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc gccccgcacc
     6721 tttggatgcg ttttggcaaa aaagctcaaa tcaccgcggg tggcaaatac acagccgact
     6781 cgcttttgcg ccaggcgacg tagcgggtaa aaaagcttat atctcaagcc actggaaagc
     6841 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6901 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6961 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     7021 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     7081 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     7141 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     7201 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     7261 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     7321 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7381 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7441 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7501 cggtaaaact cgccgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7561 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7621 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7681 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7741 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7801 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttgatc aacatgatgg
     7861 cggcaggaat caccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7921 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7981 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     8041 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     8101 caaacagtcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     8161 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     8221 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagctccg gcaagcacgc
     8281 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     8341 acacagccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8401 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8461 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8521 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagcg ttcccccgca
     8581 gggcaaccgt gcagcggaat gtaatggaac accgccatga tttccgcttc tttcagaaag
     8641 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8701 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8761 aacgcatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8821 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8881 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8941 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     9001 tcaccacccg ccgtatagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     9061 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttctaccaca
     9121 aacagattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     9181 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ccgcttcaat cagcgtttcg
     9241 tcgatgttca tggtgtccgg gcgaacatcc acaaaaacga tttttgcgcc acgcagcaca
     9301 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9361 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9421 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9481 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9541 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9601 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9661 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9721 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9781 agagacataa ccgcgaatac cgccggacgc cgcacgtaaa atcagacatt gatgatcaaa
     9841 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgtccgctgg cgtcaggcgc
     9901 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctggctaact gacgtaatgc
     9961 gggaatatcg gtctcttgtg ccactacagc accactatca ctgacattgt tcacgggtag
    10021 tgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
    10081 acccgtattt gatgccgcaa ttttggcctg cacccgtgac cacggcgcta acacgtctgg
    10141 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
    10201 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
    10261 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
    10321 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10381 ctactgtctg cacaaaggta ctggcttcaa tcaggctgtc gtgagtgcca gtgtccagcc
    10441 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10501 tgatggaggt aatctccagt tcaccacgct ccgacggctt cacctgcttt gcgtactcca
    10561 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10621 gcttttcttc cagcgagata gcgcggaaat tgtcgtcaaa ctccaccacg ccaaagcgtt
    10681 ccgggtccat cacctgatag ccaaataccg tcgccccttc cgtgcgcgcc gcaacatgac
    10741 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10801 gttcaccgtt gaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10861 gctgttcggc gtattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10921 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggatatcg gccaacatca
    10981 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    11041 cgccgcgcgt aatcggatgc aatcgggtgc cggaaccgcc cgccaggata atacctttca
    11101 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcgccctga tagctgccgt
    11161 cctgcacctg cttccaccag ctttcattag ccagatacca ctgcaccgtt ttacgcattc
    11221 cactttcaaa ggtttcctgt ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    11281 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcaag tcacgatagt
    11341 gcaccacgcc gtgcggcttg ttcggagcca gttcttccag cagctcgcaa atggtttcca
    11401 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11461 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11521 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11581 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgataa
    11641 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11701 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11761 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11821 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11881 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11941 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    12001 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    12061 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcgtcg ctcgtttcgt
    12121 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    12181 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    12241 attgataact ttaaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    12301 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12361 gttaggctca acaaccagag tttcgccgct atgccactgg gcgatcagtt cagcgatttc
    12421 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12481 gattttcagt tcactggcgc gtttatcggt agccgccagg caatcagcca ccgccgcttt
    12541 cacctgatcg ataacccaga acggtttgtg atcgttcact tcgcgcgcgg tacggataag
    12601 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12661 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12721 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12781 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12841 agtgacgaca cactcacctt cgaggaaaat tttgtacagt tcgctggcgc gggtcgaaca
    12901 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12961 cggtaacacg cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    13021 tgggaaagtg agatccggac gcatctctgc taaccattct gccatcttct cggttgaccc
    13081 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    13141 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtggg
    13201 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    13261 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    13321 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13381 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13441 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13501 ccatcaccat acgggttatg ggcgcggctc atagtttgat attcgttttc gtcttttaaa
    13561 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13621 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13681 gaaggcgctt cttcctgaat gccgcctgag tcggtcaaaa tcagccaggc gtggttcatc
    13741 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13801 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13861 atgtcctggt gcgtggtggc aatgtctgcc aacgcgtggc agatttcttc aaagccacga
    13921 ccgaaacttt cacgcctgtg accggtcacc agaatcatct ttttatcggg gtcgataaac
    13981 gggtaatttg ccgccagttc tgaacgcagc gtatcgctgc tcatcacctg gtcacgcacc
    14041 cataacagtg catcaataac tgtattacca gtaatgaaga ttcggctatc cgcaacgttt
    14101 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    14161 ccggtcaatg tacggttagc ctcttccggc cacggcgaat aaagatcgcc cgtgcgcaga
    14221 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    14281 gtcgtcgttg tgtcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    14341 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14401 ttgagatcgt agtcaggtac aatggaaaag agtttcagca cctgatcgag catctcccga
    14461 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14521 aacggcgcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14581 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14641 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14701 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14761 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14821 ccgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14881 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14941 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgttcaat
    15001 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgtgc
    15061 tttcatctgg atggtacgcg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    15121 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    15181 ttcagcaata agcttcacgc tgtcattgac cgcgcgggta aagtctccgg ggataaactg
    15241 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    15301 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15361 cataacaaac tctttatagg cttcgtccat gaccgatggt tggtcggcag aagccatgtt
    15421 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15481 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15541 cgccagcgcg attaacgcaa atgccagccc catgccaata atccatagct tcccagccca
    15601 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15661 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15721 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15781 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15841 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgccgc agcaagggta
    15901 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15961 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    16021 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    16081 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    16141 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    16201 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    16261 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    16321 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16381 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16441 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16501 tttacgctga tatcaaaacg gtcatccagc gcgccaatga aaacaagcac accggcacaa
    16561 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16621 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16681 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16741 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16801 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16861 gtccgttccg aacccaccag tcctgtgcaa aaattaacca tttgatatgc aaattgcata
    16921 acacgccagc aaattccagg aattttccta acatcgccag cgcggcatta agattacaga
    16981 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    17041 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    17101 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    17161 gcagttcttc ctgagtagtg agcagttctt ctttacgggt accggaacgg ttgtagtcga
    17221 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    17281 tacctttaaa ctcttcgtag ataacttcgt ccattttaga accggtatcg ataagcgccg
    17341 tcgcgataat agtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17401 tcggacgatg cagggcgttg gcatccacac caccggtcaa cactttacct gacgccggaa
    17461 caacggtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17521 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17581 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17641 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17701 tgtaagcaat gctctgggca atgttctgca gcagcatggt tttaccggct ttcggcggtg
    17761 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17821 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagaattt gcgtgcagcg
    17881 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17941 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    18001 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    18061 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    18121 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    18181 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    18241 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    18301 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18361 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18421 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18481 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18541 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18601 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18661 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18721 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18781 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18841 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18901 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18961 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    19021 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    19081 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    19141 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    19201 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    19261 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    19321 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19381 tgccgacgca gaaccgctgg cggaagctac tggcctgaag ctgggtctgg cttacggtgg
    19441 tgatggctac gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19501 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19561 agtggttctg gatgaagccg atcgcatgta cgatctgggc tttattaaag atatccgctg
    19621 gctgttccgc cgtatgccgc ctgcaaacca gcgcctcaac atgctgttct ccgccacgct
    19681 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19741 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19801 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19861 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19921 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19981 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    20041 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    20101 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    20161 tgaagagtat gcattgaatt tgcctgctat tgaaacctat attggtcact caattccggt
    20221 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    20281 cccacgtaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    20341 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20401 cccgatgagc atactgtagt aagtgattca ggtgaaagaa tgcagccaac acagagacag
    20461 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20521 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20581 gaataaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgcc ctgtctaatg
    20641 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20701 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg tcttgccgtc aatgcgggtg
    20761 attttattgc caatgcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20821 aggaagcacg tctgatttat cagggcgttg ctcacacaac tggcggtgcc gatcagcgcc
    20881 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20941 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    21001 gtaatctggg gcaggaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    21061 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    21121 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    21181 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    21241 acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca
    21301 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21361 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acgctgcgta
    21421 atattcagcg ccgctttatg atcgatattg atcaggcgca gcgcgtagcc aaagttgcgg
    21481 ctaacttctt cgatcaggtg gaaaaggaat ggcatcttga agcaataagc cgcgatttgc
    21541 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgactttaaa caagcgccgc
    21601 aacacgctgc ttatctggtg cgtaatctgg atcttcccgg ttttaccccc gcacagaaaa
    21661 aattgctggc gacgctactg ctcaaccaga ctaatccggt cgatctctca tcgctgcatc
    21721 agcaaaatgc cgtaccgccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcca
    21781 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21841 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21901 agattattgc tcaggaaagc cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21961 aatgagtaag tgccggatgc gatgctgacg catcttttcc ggccttgatt atttccctcg
    22021 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    22081 ttcggcgctg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    22141 caaaaagcgg ctcggctccg ggcgcaccag ctcgccgtac tgacggcgtt ctttacacag
    22201 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    22261 atcgatatta tcttcatcga tgctgctctg gtgcggcaaa aacccttctt ccataccgac
    22321 catgtagaca taaggaaact ccagcccttt tgacgcgtgc agagtcatca gttgcacctg
    22381 atccagctct tcttcactct caccacgctc catcatgtcg cgcaaagtaa agcgcgtcac
    22441 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22501 gctaaacagt tggttgacgt tcttcatgcg catttcggcg gctttcgggc tgggcgatgt
    22561 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22621 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtaaagcggg tcaatgcttc
    22681 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22741 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctct
    22801 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22861 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22921 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac actcgcgact gatggttacc
    22981 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    23041 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa ctttaagctc
    23101 tgtgccgtaa cccagttcgg agaacagacg cttctcaaag acgtgcgggt tattggcgat
    23161 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    23221 caccttcagc gccggaaaat cctgactcag cagcaccagg ttttgtggac gtgcgccgcg
    23281 ccaggagtag atcgactggt cgtcatcgcc caccacggta aagcgcgcgc ggctgcccac
    23341 cagcagtttc accagttcat actggctggt gttggtgtcc tgatactcat ccaccagcag
    23401 atagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gcagcaacgt
    23461 cggcaataaa atcagatcat cgaagtcgag aacattgcac gctttcaggt gtgcatcata
    23521 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23581 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23641 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23701 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23761 atccagcccc agcgtgtgga aggttgagat catcagccca cgcgcctctt tgcgacccag
    23821 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23881 gatatgtcgc gcctgataac cgcaaccgcg gatcagatgg gcgattttat tggtgatcac
    23941 acgagtttta ccggaacccg cgcccgccag caccaggcag gggccggtaa cgaattcgac
    24001 agcttgttgt tggccggggt ttagacgcat aggtgtattg ctcaatcttc gaacgggggg
    24061 aggattgtag cagaatcgca tttgaatgtg accgcgtgta agccgctaac cgattggctg
    24121 cggatgttta aatcagagag ggaagaccga aaccggagtt catcgtgaat ccattagact
    24181 tagaaaatat cgggtcgtaa cgttgtgtta ttactttttc agagtttact aaggagaggt
    24241 gtactcgtgg cgattaattt cagtcctaag gttggtgaaa tactggattg caactttggt
    24301 aactatcctg ttagccagaa tggacagttc agcaccacgt attacgatgg ccgcattcct
    24361 cctgaaatga taaaaaacag gttagt
//
LOCUS       DAZOZY010000046        23863 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZOZY010000046 DAZOZY010000000
VERSION     DAZOZY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.046"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(89..1342)
                     /gene="amiC"
                     /locus_tag="ACHURQ_001597"
     CDS             complement(89..1342)
                     /gene="amiC"
                     /locus_tag="ACHURQ_001597"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR0823404.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1574..2905
                     /gene="argA"
                     /locus_tag="ACHURQ_001598"
     CDS             1574..2905
                     /gene="argA"
                     /locus_tag="ACHURQ_001598"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR0823405.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2967..4793)
                     /gene="recD"
                     /locus_tag="ACHURQ_001599"
     CDS             complement(2967..4793)
                     /gene="recD"
                     /locus_tag="ACHURQ_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR0823406.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4793..8335)
                     /gene="recB"
                     /locus_tag="ACHURQ_001600"
     CDS             complement(4793..8335)
                     /gene="recB"
                     /locus_tag="ACHURQ_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR0823407.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8328..11216)
                     /gene="ptrA"
                     /locus_tag="ACHURQ_001601"
     CDS             complement(8328..11216)
                     /gene="ptrA"
                     /locus_tag="ACHURQ_001601"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR0823408.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11392..14760)
                     /gene="recC"
                     /locus_tag="ACHURQ_001602"
     CDS             complement(11392..14760)
                     /gene="recC"
                     /locus_tag="ACHURQ_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR0823409.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14773..15096)
                     /locus_tag="ACHURQ_001603"
     CDS             complement(14773..15096)
                     /locus_tag="ACHURQ_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR0823410.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15081..15488)
                     /locus_tag="ACHURQ_001604"
     CDS             complement(15081..15488)
                     /locus_tag="ACHURQ_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR0823411.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15485..16048)
                     /locus_tag="ACHURQ_001605"
     CDS             complement(15485..16048)
                     /locus_tag="ACHURQ_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR0823412.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16039..16509)
                     /locus_tag="ACHURQ_001606"
                     /pseudo
     CDS             complement(16039..16509)
                     /locus_tag="ACHURQ_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16694..17488)
                     /gene="thyA"
                     /locus_tag="ACHURQ_001607"
     CDS             complement(16694..17488)
                     /gene="thyA"
                     /locus_tag="ACHURQ_001607"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR0823413.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17495..18370)
                     /gene="lgt"
                     /locus_tag="ACHURQ_001608"
     CDS             complement(17495..18370)
                     /gene="lgt"
                     /locus_tag="ACHURQ_001608"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR0823414.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18521..20767)
                     /gene="ptsP"
                     /locus_tag="ACHURQ_001609"
     CDS             complement(18521..20767)
                     /gene="ptsP"
                     /locus_tag="ACHURQ_001609"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR0823415.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20780..21310)
                     /gene="rppH"
                     /locus_tag="ACHURQ_001610"
     CDS             complement(20780..21310)
                     /gene="rppH"
                     /locus_tag="ACHURQ_001610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR0823416.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(21663..21809)
                     /gene="ygdT"
                     /locus_tag="ACHURQ_001611"
     CDS             complement(21663..21809)
                     /gene="ygdT"
                     /locus_tag="ACHURQ_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR0823417.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21995..22684
                     /gene="mutH"
                     /locus_tag="ACHURQ_001612"
     CDS             21995..22684
                     /gene="mutH"
                     /locus_tag="ACHURQ_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR0823418.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22753..23466
                     /locus_tag="ACHURQ_001613"
     CDS             22753..23466
                     /locus_tag="ACHURQ_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR0823419.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23604..23822
                     /gene="ygdR"
                     /locus_tag="ACHURQ_001614"
     CDS             23604..23822
                     /gene="ygdR"
                     /locus_tag="ACHURQ_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR0823420.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT         5706 a         6533 c         5961 g         5663 t
ORIGIN      
        1 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct actctcccat
       61 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg
      121 caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
      181 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
      241 gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct
      301 tgttgatttt accgagctta ttcagcactg ctttaccaaa cttaaggctg tcagcaatgg
      361 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
      421 cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg
      481 ttgcaccttt ggttgagagc gcaaacaccg aggaaccgct cggctggcga ctggtaaagg
      541 cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca
      601 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
      661 tctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt
      721 tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa
      781 tcggacgatc gcgccctgct ttacctggtt gtggaccact ttgcgctggc ggcacctgct
      841 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
      901 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
      961 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatccgt acggtttgcg
     1021 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
     1081 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag
     1141 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg
     1201 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga
     1261 cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga
     1321 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
     1381 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
     1441 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
     1501 tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcga ataatcatgc
     1561 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc
     1621 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
     1681 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
     1741 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca
     1801 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
     1861 ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt cgatgagtct
     1921 caataacacg ccgctgcagg gcgcgcatat caacgtcgtc agtggcaatt ttattattgc
     1981 ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat
     2041 tgatgaagac gcgatccgtc gtcaactgga cagcggtgca atagtgctaa tggggccggt
     2101 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
     2161 ggccatcaaa ctgaaagctg agaagatgat tggtttttgc tcttcacagg gcgtcactaa
     2221 tgacgacggt gatattgtct cagaactttt ccctaacgaa gcgcaagcgc gggtagaagc
     2281 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
     2341 agcctgccgc agcggcgtgc gtcgctgtca tttaatcagt tatcaggaag atggcgcgct
     2401 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
     2461 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
     2521 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
     2581 attcaccatt attcagctcg ataacacgac tattgcctgc gccgcgctct atccgttccc
     2641 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
     2701 acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa
     2761 attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt
     2821 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
     2881 agtgttgatg gcggatttag ggtaagcgat gaaaatcgcc ggatgcggca ggcgtaacac
     2941 tcgtgcgtcg catccggcaa ttacgtttat ccccgcgaac taaacaacgc cgccagacca
     3001 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc
     3061 gacagacggc gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactatcggc
     3121 gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
     3181 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
     3241 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
     3301 atatcgccat taaacaaccc aagcgcactg tcattacggg caatcatcac cggtcgacct
     3361 tcgtaccaac gagagtgcga atgacgatga attttgcgct tctgttgcat aaactgctca
     3421 attcgctcat tcagtccagc cacgccaaat ggcccttccc gcagggcgca caaaagttgg
     3481 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
     3541 taacgtccat aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt
     3601 aaaagccgtt tttcaatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
     3661 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag
     3721 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc
     3781 ggaacgtgag ttcccgtcag gcggcttagc tgcccggcac gctcggcggt aaagccggca
     3841 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
     3901 cgatcgccga gaaagatcac tcgcgcatga tcgggcaagg cgtcgatcag tctcgacatc
     3961 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
     4021 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg
     4081 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
     4141 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
     4201 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc
     4261 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgct
     4321 gccgccactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
     4381 gccagtagag cttcatcaac ctcaatggca tgattcacct cattgaagaa gcgcgctacc
     4441 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
     4501 gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt
     4561 agctcaccga tttcactgac acaggtcgcc aacagcggat gcgacgcctc gttattttcc
     4621 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
     4681 gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc
     4741 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
     4801 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
     4861 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
     4921 taacgccgcc aaagtgacga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
     4981 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
     5041 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
     5101 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
     5161 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
     5221 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
     5281 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct
     5341 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
     5401 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa
     5461 acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg
     5521 tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat
     5581 cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat
     5641 cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct
     5701 gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
     5761 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
     5821 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
     5881 gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca
     5941 gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat
     6001 ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat
     6061 tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga
     6121 caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt
     6181 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
     6241 ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
     6301 ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg
     6361 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat
     6421 cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg
     6481 acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
     6541 gccaaagcat ttcctgcgct tccagagttt caaaaacact atcgcggttc gaaaggtaaa
     6601 cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc
     6661 gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
     6721 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
     6781 ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
     6841 tcgcaggctg cgtttcacct ttaaagacaa aacgtaacgc ctgatttttc ccggctgatt
     6901 tcactggaat aaacggtatt tcgcgaaaca tgaacgcgtc atcagtctgg ctgaaaagct
     6961 tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg
     7021 cgtgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
     7081 tggcctgctt cgggtcgcca atcagcaaca atgcggtttc tggctgatgg tgccagatac
     7141 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg
     7201 ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat
     7261 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
     7321 ctgtttcgcg gatctcagcc aatgcgcggg tgatcaccag atcacggatc gacaatggtt
     7381 ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggctt
     7441 tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac
     7501 tgtttgtctc ttcttctgcc caggcgctga tcttgtcgat ccatttagcc tgattgctac
     7561 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
     7621 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tgacgggaag
     7681 ctagcgtttc atcatcgggc ggcggtgctt tgataaccgg cgcttcgcct tgcagataac
     7741 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta
     7801 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
     7861 gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca
     7921 ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc
     7981 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggt
     8041 gttcgtacaa tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
     8101 tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
     8161 gttcttcaac ggtcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
     8221 gcaaataaag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
     8281 gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
     8341 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
     8401 tcaggatgca catattcggc tttcccgttt tggctgccgg aaatctgcga caggatagcc
     8461 atgccttgcg gctcgactac cgcctgatgg aagaaatcgg ccagtttttg cggcgtcagc
     8521 agtttaatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
     8581 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
     8641 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
     8701 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
     8761 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
     8821 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
     8881 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
     8941 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
     9001 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
     9061 tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt
     9121 cgagcccctg attttaaggc gtcgcgataa gccagcacct ctttcaacgt aatggagggc
     9181 aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
     9241 ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag
     9301 gacttcgcct gctcaagctg atcttccgta gcggtatagc taaagtaccc ctcgagcaat
     9361 gcctggaaca gctgcggcag acgctggggg taaccattag cattaaccat aaggccgttg
     9421 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
     9481 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
     9541 gctttcggat tacgcaaaat caggctgaca tcagctttgg gttcgctgga aaaataacgg
     9601 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcatat
     9661 ttcttctctg acttaatcag cgagaaatcg tcaggaatat aggggttaag ctccggtaag
     9721 gagagcgcaa tattggcagc tttttgctgc cagtcagcga aagtttgttc gctgatttta
     9781 tcgacctgat acggcgcatc gacaaaataa gccgttttat tgtgcggctc tttcgggctg
     9841 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcttt tactgcttta
     9901 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaaccgg aacgcgaatc
     9961 atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag
    10021 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt
    10081 aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta
    10141 tcggttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    10201 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcggaaag tgtacctggg
    10261 ctgcgattac caatcagata ggtaatcaat tcatccgttt tactacggaa cttcgctgag
    10321 ttgttatcga tgcgaaactc aacgcgcaga actttacgcg gcattgccgg gacgtaatga
    10381 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    10441 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    10501 ttattgctgt aaataaccgc cttcatcaga ttggcggaat agtacttctc gtggaaatct
    10561 ttcagcgcct gctgcaccgg attaccaggt ttatcgctta aagtttcgag gttaccacca
    10621 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    10681 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ccgcattacg ctcacgttcg
    10741 gcatatttct tgtcgagcaa aggttcagca atagcatcgg ccaggcggtc taccgcacca
    10801 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    10861 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    10921 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    10981 tcttccagcg acccaacggg caccaccagc gccgagagcg atttaactgc ctgcggatca
    11041 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    11101 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaag
    11161 ggtgcccaaa gggcaactaa caacaataat gctttgaacc aggtgctgcg gggcattctc
    11221 ggacctcata atcaacttaa ttttctgtcc agattcaaca cgttaacgca aatcatctgc
    11281 cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac
    11341 tggtgcgcat cataaagtaa gcggatagat tgcgcaattt ttatacagca ctcatgactg
    11401 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    11461 ctctggtgtt aattgccgcc agagcctttg ataccagata tcatcacctt cgccacgcac
    11521 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    11581 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    11641 ttcaggtaac accagcaatg gcgcggacat cccttcacga tacccctcaa tcagttgtga
    11701 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcgc cgtctttgcg
    11761 tagaaaaagg cgactttcac cattaccacc gctggcacag tagacaaggt gttccagcca
    11821 aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    11881 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    11941 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    12001 ctgagtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    12061 gcgacggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    12121 gatttgataa cggctaagcc cttccagaat aaatggctcg gtgtcgggga tttcgctgtc
    12181 ttcagtacgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca
    12241 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    12301 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct
    12361 ttgtcgttcg cctggctgat agttttgtgg atcaaacggc attcgggtat ggaggcaagt
    12421 aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag
    12481 ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc
    12541 actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat
    12601 taacgcttcc aggaacagat agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    12661 tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt
    12721 cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat
    12781 gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag
    12841 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    12901 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    12961 caggaagaag gcgctgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc
    13021 ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac
    13081 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    13141 cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg
    13201 tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc
    13261 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    13321 cgccagcacc ggcacatcca gtaatgccag cacgtcttcc gacacaaagc ggctgtctgg
    13381 caacgataac aggctgataa acgcttccag taccggatgc gactgccgcg cccgacggtc
    13441 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacacag cccgaataaa
    13501 cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    13561 gtcttcttcc agcatcgcca gcaggcgatc gtgcaaaact tcaacttcac gctgcgggct
    13621 atggcaaacg tggaaggtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    13681 acgggaaaac tcttcgatgt tcacaccagc aacggcacgg ttttccagtt ccagaatgtc
    13741 agactgaatg ttatgcagca ggttatctgg cgtgacatcg acaaaggcgt ccagctcctg
    13801 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttac cccatgaagc
    13861 cagcagcggg ttgccgacat cctgttcacc atcgctgtta aagagctgcc cggcattttc
    13921 gctatcacga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgggtcag
    13981 cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc aggggttggt
    14041 aaacaggaga tggatttcaa tatgtttacc cagcgcctgt agcgcctgga gataaacagg
    14101 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt
    14161 cgcggactcc agcgtttcga taaagcgctg atagagattg gcgcggtgcc agcgcggttg
    14221 cccgagttga tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc
    14281 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    14341 gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt
    14401 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg
    14461 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    14521 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc
    14581 aatgtttgcc gcaatactaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    14641 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    14701 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    14761 agcggctcct gactactgac gattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    14821 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    14881 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg
    14941 ccgccagagc tgttggtact ggtttctgct ggcgagactg ttcattaatg ttcgctggat
    15001 acccgataac gcagtgacaa tcatcaccat cagcaccatc gcaaccatta cctccggcag
    15061 gctaaagcct tgttgattct tcagggaagc tgacataacg ccctctcttt cagcggacaa
    15121 aaatcgctcc agccgcgtgg cgaaaaaaca atgtttccat caatgacttc gcctgttcgc
    15181 cacaacgaaa cgccttcata accggcaacc aataaggctt cattatctgc cagtaaacgc
    15241 aaacaaaccc gggcatcggt tccagcgtac tgcgagcact gaactgcggg ctgcgtctgc
    15301 caggagtgca ttttccccca cgccagcgcc gactgaacga tggcctggcg gcgcaatgac
    15361 tgactttcta tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat tccttgcaat
    15421 agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac acctttttcg
    15481 cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt tgcggttcag
    15541 acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg acatcctgac
    15601 gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta tcccagccct
    15661 taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc agacgaaatc
    15721 caatctggtc agactctttt accggttcgc gatcccagat accgttactg ttcgcatccc
    15781 actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg gtacagctgc
    15841 catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc agccagattt
    15901 catcttccag cgccagctta cgggtgctcg ttaaactttc acgctgtaac gcaggcagaa
    15961 agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac acttccagca
    16021 gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgac aaagccggag
    16081 tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga agcgaatatg
    16141 cccggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca ggtcgctcat
    16201 ttagacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac aggtattggc
    16261 cccagcaacg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc tgatactgtg
    16321 gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc gcgcctggct
    16381 ggcggtttgc catagccgtt gcgactgctg ccagtattgc cagccataga gtccacttgc
    16441 gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat aaccacgttg
    16501 tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag tttctatttc
    16561 ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt tcgggaaggc
    16621 gtctcgaaga atttaacgga gggtaaaaaa aaccgacgca cactggcgtc ggctctggca
    16681 ggatgtttcg taattagata gccaccggcg ctttaatgcc cggatgtgga tcgtagcctt
    16741 caatctcaaa gtcttcgaaa cggtagtcga agatggattc gggtttacgt ttgataatca
    16801 acttcggcag cggacgcggt tcgcggctta attgcagatg agtttgatcc atatgattgc
    16861 tgtacaggtg cgtatcgcca ccggtccaga caaaatcacc cacttccaga tcgcactgct
    16921 gcgccatcat atgcaccagt aacgcgtagc tggcaatgtt gaacggcagg ccgaggaaga
    16981 cgtcacagga gcgctgataa agctggcaag agagtttgcc gtctgccaca tagaactgga
    17041 agaatgcatg gcacggtgcc agcgccattt tatccagttc gcctacgttc cacgctgaaa
    17101 caataatgcg gcgggaatcc gggtcgtttt tcagctggtt cagtaccgta gtgatctggt
    17161 caatatgacg accatctggc gttggccagg cgcgccactg tttaccatac actggtccga
    17221 ggtcgccgtt ttcatcggcc cattcgtccc agatggtgac attgttttcg tgtagataag
    17281 caatgttagt gtcgccctgc agaaaccaca gcagttcgtg gatgatggaa cgcaggtggc
    17341 aacgtttagt tgtcaccagc gggaatccat cctgcaggtt aaaacgcatc tgatgaccaa
    17401 aaatggaaag cgttccggtt ccggtacggt cgtttttctg tgtgccttcg tcgagcactt
    17461 tttgcatcag ttctaaatac tgtttcatgg ttcctcagga aacgtgttgc tgtgggctgc
    17521 gacgatatgc ccagaccatc atgatcacac ccgcgacaat catcgggatg gaaagaattt
    17581 gccccatgct gatgtactgc acccaggcac cggtaaactg cgcgtcgggc tggcggaaaa
    17641 actcaacaat gatgcgaaac gcgccgtagc caatcaggaa caagccagag acagctccca
    17701 ttgggcgcgg tttacgaata tacaggttga ggataataaa cagcaccaca ccttccagca
    17761 gcagctcgta aagctgtgat gggtggcgcg gcagcacacc gtaagtgtcg aaaatggatt
    17821 gccactgcgg gttggtttgc agcagcaaaa tatcttctgt acgggagcct gggaacagca
    17881 tggcaaacgg gaagttcggg tcaacgcggc cccacaattc accgttaata aagttgccca
    17941 gacgcccggc accaagacca aacggaatga gtggtgcgat aaaatcagaa acctggaaga
    18001 aggaacgttt agtacggcgg gcaaagataa tcatcaccac gataacgcca atcaggccgc
    18061 cgtggaaaga catgccgccg tcccagacac ggaacagata cagcggatcg gccataaact
    18121 gcgggaaatt gtagaacaga acataaccaa tacgtccccc gaggaagacg ccgaggaagc
    18181 ccgcatagag taagttttca acttcatttt tggtccagcc gctgcccgga cgattcgccc
    18241 gtcgtgttgc cagccacatt gcaaaaatga aacccaccag atacatcagg ccgtaccagt
    18301 gaagcgccac gggtcctatt gagaaaatga ccggatcaaa ctccggaaaa tgcagatagc
    18361 tactggtcat ctgtcaccac aagttcttgt tatttcgctg aaagagaaca gcgattgaaa
    18421 tgcgcgccgc aggtttcagg cgctccaaag gtgcgaataa tagcacaagg ggacctggct
    18481 ggttgccgga taccgttaaa agatatgtat atgatccgcg ctataaccct ccgcgaatca
    18541 gcccgcccat gccgcgacgc tccataaagg ctgcaacctg atggcgaact tcggtcgcca
    18601 gttgcgcttc cagactacgc tgcgcaagat tttctgcttc ggcataatca atgcgccgca
    18661 gcaggtattt tacccgcgct acagaacgtc cgttcataga cagatggcga tacccaagcc
    18721 caatgaggat tgccacgcac atgggatcgc ccgccatttc accgcacaaa cggagatcga
    18781 ttccatgtat ttccgcttcc cgggcgatca tcgccagagc tcgtaacatt gcaggatgaa
    18841 gactgtcata aatgttcgcc acccgggtat tgttgcgatc aacggccaga atgtattgag
    18901 tcagatcgtt ggtgccaaca gagatgaaat cgacccgctt tgccagatgc ggcagcataa
    18961 ataccattga cggcacttcc agcatgatgc cgatacgtgg tttgggaatt tcgtaaccga
    19021 tcatctcctc gacttcacgt ccggcacgtt caatcaggcg gcgtgcttca tcaacttcat
    19081 cgaggcttgt gaccatcggc aacagaatat tcaggttgcc cgtagcggca ttagcacgca
    19141 gcatcgcccg cacctggatc aagaagatct ccggctgatc gagcgtaatg cgaatcccac
    19201 gccaacccag gcatggattc tcttcgctga tcggcatgta aggcagctgc ttatctgctc
    19261 cgacatccag cgtacgcaag gtgacgggtt tatcattgaa catttgcagc atcccctgat
    19321 actgcgccac ctgttcttct tccgacggga aaccactttg cagcatgaat gggatttcag
    19381 tgcgataaag accgatgcca tcaatacggc tgcccagttt ttcttcatgt tccgggctta
    19441 aaccagcatt gagcatgact tttatacgct caccgctttt taactgcgcg ggtaaattga
    19501 cgtcatcttc cgccagacgg ctaagctcaa tctcttcact aattagccgc tgatattctt
    19561 gcagcagtac cggctccgga tcgaccagca attcaccgcg atagccatca acgatcagcg
    19621 tcctacgatg cagcaccgaa ggctgaatat ccgcgcccat cacggtaggg atccccagcg
    19681 cacgtaccat gatcgcagca tgggagttgg ctgcgccatc tcgcacgaca acaccgacta
    19741 agcgatcctg gggcagctca gcaagagttg tcgctgacag ttcatctgcc accagaatga
    19801 aacgttccgg ccaggcgttc ggcccttgat tagcgtcatc aagatgaaac agcaatcgct
    19861 gacccagcgc acgtaaatcg ccagcccgct ctttgagata gttatcgctt agcgcggcaa
    19921 actgttcggc aaatttttca atgaccgttt ttaccgccca ctctgccacc gagcctttat
    19981 caacctcggc aaacaattcg cgacgcagcc gggtgtccga aagtaggtgc gagtaaagat
    20041 cgaaaatagc cgccgtttct ttttgtgctc cggcggcaaa gcgtttgctg tagcggcgaa
    20101 actcgtttgc cgcttcttcc agcgccccgg tcagtcgttc gcgttccaga gccggatcca
    20161 gcgttgatgc ctgatacacc tgttccatta aaggtaacgt ggcatcctgc cagccttcgg
    20221 caatcgccac accaggtgct gccggtaatg cgcggattcg cgtctggcga tattgcccaa
    20281 acaaggcagt caactgcgac tgagaaagaa tagctgccat ctgggtggca agcgtcacca
    20341 ggaaggattc ttcactttcg tcatactggc gcaactctcg ttgctgtacc accagtacac
    20401 caagcaactg gcgacgttga ataattggta cgcctaaaaa cgcgcggaaa cgttcttctt
    20461 ttacggaggg gatgtatttg aagctggggt gcttttgcgc atctgcaagg tttatcggtt
    20521 ccgccagcct gccaaccagg ccgacgatcc cttcatcaaa cgcgagcgtt acagtgcgac
    20581 cgcgtggttt tttcagcccc cgggtcgcca tcaggtagta acaacgtcga tcatgatcgg
    20641 ccaggtagac cgaacagacc tcggtatcca tcgcaagaca gatgtcggta accagaatat
    20701 ttaacgcctc attcaggcgt ggtgcgctgg ctaccttttc gactatttcg cgcaggcgag
    20761 tgagcataat tggcgtgact taacctcttt tacgtcgata agcagatgtg ttttgtggtt
    20821 ttggcgtatt ttcctgcagt gacatcacca cactcgcgaa ctctttcatt accctacggt
    20881 agacatcacg tttaaatgac accacctgtc tgaccggata ccagtaactt acccatcgcc
    20941 agccgtcaaa ctctggtgta ctgctggttt gcatattgat ttctgcatcg ccgctcacca
    21001 gctgcaagag aaaccatttt tgtttttggc cgatacaaac cggcttcgtg tcccaacgca
    21061 ccaaacgttt cggtaatttg tagcgcaacc agttacgcgt tgaagcaagg attcgaacgt
    21121 ctttgcggct taatcctact tcttcaaaca attcacggta catcgcctgc tctgcggatt
    21181 ctccggggtt gattccgcct tgcggaaatt gccaggagtg ctgaccaaat cgccgggccc
    21241 acattacctg cccctggcga ttacaaatca cgatacctac gtttgggcgg tagccatcgt
    21301 catcaatcac cggactacct caaaataaag ctttatatac gaatgattgt ttcatactcc
    21361 aagaagacgg taaaccactc tctgcagggc attacacact aataacaatt gaataactca
    21421 cagttatgtg cagagttata aacagaggaa ggggtggata gccgttttta ttcacctttt
    21481 ctgtggatag agttgtgaag aactacggaa ttactacggg aaaacccgga gcattctgaa
    21541 taagcccgat atagacattt taaaatattc attattagtc aatgcattac atgtaatttg
    21601 agtaatgaaa aagagtaatt cgtgacccag gtcacacctc tcatttacgg gttggcgaaa
    21661 gatcaaccaa tgccgtattt atccacagaa tgtgccacta agttaagcac tgaaccacta
    21721 aaaactggag tttcgtcgca cgtcaaggct gtaaatggaa acagtagtgg aggtttttca
    21781 cagttatccc agctttctgt ggataacatg gtgtaagatc ctgtttattt tcagtgacca
    21841 gatttggaaa acctattgca gtgttgcgca actcgattac cggcagccta aaaagcaata
    21901 taaatcagag aattgaacaa cacatgtgga aaaagttaca ctgcgaatat tcggcacata
    21961 attgctgttt gttttttaac caaggtatca tgacatgtcc caacctcgcc cactcctctc
    22021 tcctcccgaa actgaagaac agttgttagc gcaagcacag caactttctg gttatacatt
    22081 gggagaactg gcggcacttg ccgggctggt tacgccagag aatttaaaac gcgataaggg
    22141 ctggattggc gtgttactgg agatctggct aggtgccagc gcagggagta aacctgagca
    22201 agattttgct gctctgggcg tggaacttaa aactatccct gtggatagtc ttggtcgtcc
    22261 gctggaaaca acattcgttt gtgttgcccc gttaacgggt aatagcgggg tgacctggga
    22321 aaccagccat gtgcgccaca agctcaaacg cgtactgtgg ataccggttg aaggtgagcg
    22381 cagcatcccg ctggcgcagc gtcgcgtagg atcaccgttg ctgtggagcc cgaatgaaga
    22441 ggaagaccga cagctacgcg aagactggga agaattaatg gatatgattg ttctcggtca
    22501 ggttgagcgg atcaccgctc gtcacgggga gtatttacag atacgaccga aagcagcgaa
    22561 tgcgaaagcg cttaccgaag ccattggtgc ccggggcgaa cggattctga cgctgccacg
    22621 cggcttttat ttgaagaaga atttcaccag tgcactactg gcccgtcatt ttctgatcca
    22681 gtagccatcg ctttgacctg ccgctttccg ggcatataat taccgcttca tttttttggc
    22741 agggcttttt agatgttatt tgcatggata accgatccta acgcctggct tgcgctcggt
    22801 acgctgacgc tgctggagat cgttcttggg atcgacaata ttattttcct ttctctggtg
    22861 gtggcaaagc tccccacagc acaacgtgct catgcgcgcc gtctggggtt ggcgggagcc
    22921 atggttatgc gtctggcgct gctggcatca atcgcctggg ttacgcgcct gacaaatccg
    22981 ctttttacaa tattcagtca ggaaatttcc gcccgtgatt tgattctgct tctgggtggc
    23041 ttgttcctta tctggaaagc cagcaaggaa atccacgaat ccattgaagg tgaagaagaa
    23101 gggctgaaaa cacgcgtttc atcattcctc ggcgctatcg tgcagattat gctgctggat
    23161 attatcttca gcctcgactc ggtgattacc gctgtgggtc tgtcagatca cctgtttatt
    23221 atgatggccg ccgtggtgat tgccgtaggc gtgatgatgt tcgccgcgcg ctcgattggt
    23281 gattttgtcg aacgccatcc ttcggtaaaa atgctggcgc tctctttcct gattctggtc
    23341 ggctttaccc tgattctgga aagtttcgac atccacgtac cgaaaggtta catctacttc
    23401 gcgatgttct tctctattgc ggttgaaagc ctcaacctga ttcgcaacaa aaagaacccg
    23461 ctctgatact ccgtacgctc tcctgcggga gggcgtactc actcctgtta ccctcacagt
    23521 ttaagatttt cctgctttca agatatataa cgtcggttta taaacagact attatcatag
    23581 gtgggccaaa tgaggataac cggatgaaaa aatgggcagt aataatttct gcagtcggac
    23641 tggcgtttgc tgtttccggg tgttccagtg attacgtcat ggcgaccaaa gatggccgta
    23701 tgattttgac cgatggaaaa cctgaaattg atgatgatac cgggctggtg agttatcacg
    23761 atcagcaagg taacgcgatg caaattaacc gtgatgatgt ttcgcaaatt attgaacgtt
    23821 aacaaataag gtcagcatcc ggctgtaatg ccgatcagtt aag
//
LOCUS       DAZOZY010000047        23237 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZOZY010000047 DAZOZY010000000
VERSION     DAZOZY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.047"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHURQ_001615"
     CDS             complement(40..240)
                     /gene="glgS"
                     /locus_tag="ACHURQ_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR0823421.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            509..1138
                     /locus_tag="ACHURQ_001616"
     CDS             509..1138
                     /locus_tag="ACHURQ_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR0823422.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1165..2826
                     /locus_tag="ACHURQ_001617"
     CDS             1165..2826
                     /locus_tag="ACHURQ_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR0823423.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3065..3124
                     /locus_tag="ACHURQ_001618"
     CDS             3065..3124
                     /locus_tag="ACHURQ_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0823424.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHURQ_001619"
     CDS             3441..3500
                     /gene="ibsE"
                     /locus_tag="ACHURQ_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR0823425.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHURQ_001620"
     CDS             complement(3620..5053)
                     /gene="hldE"
                     /locus_tag="ACHURQ_001620"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR0823426.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHURQ_001621"
     CDS             complement(5101..7941)
                     /gene="glnE"
                     /locus_tag="ACHURQ_001621"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR0823427.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHURQ_001622"
     CDS             complement(7964..9265)
                     /gene="ygiF"
                     /locus_tag="ACHURQ_001622"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR0823428.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9507..10127
                     /locus_tag="ACHURQ_001623"
     CDS             9507..10127
                     /locus_tag="ACHURQ_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR0823429.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10191..11429
                     /gene="cca"
                     /locus_tag="ACHURQ_001624"
     CDS             10191..11429
                     /gene="cca"
                     /locus_tag="ACHURQ_001624"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR0823430.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHURQ_001625"
     CDS             complement(11592..12413)
                     /gene="bacA"
                     /locus_tag="ACHURQ_001625"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR0823431.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHURQ_001626"
     CDS             complement(12504..12872)
                     /gene="folB"
                     /locus_tag="ACHURQ_001626"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR0823432.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12977..13594
                     /gene="plsY"
                     /locus_tag="ACHURQ_001627"
     CDS             12977..13594
                     /gene="plsY"
                     /locus_tag="ACHURQ_001627"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR0823433.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHURQ_001628"
                     /gene_synonym="dan"
     CDS             complement(13607..14539)
                     /gene="ttdR"
                     /locus_tag="ACHURQ_001628"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR0823434.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHURQ_001629"
     CDS             14746..15657
                     /gene="ttdA"
                     /locus_tag="ACHURQ_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR0823435.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHURQ_001630"
     CDS             15654..16259
                     /gene="ttdB"
                     /locus_tag="ACHURQ_001630"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR0823436.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHURQ_001631"
     CDS             16308..17771
                     /gene="ttdT"
                     /locus_tag="ACHURQ_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR0823437.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHURQ_001632"
     CDS             complement(17814..18827)
                     /gene="tsaD"
                     /locus_tag="ACHURQ_001632"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR0823438.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHURQ_001633"
     CDS             19064..19279
                     /gene="rpsU"
                     /locus_tag="ACHURQ_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR0823439.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHURQ_001634"
     CDS             19390..21135
                     /gene="dnaG"
                     /locus_tag="ACHURQ_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR0823440.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHURQ_001635"
     CDS             21330..23171
                     /gene="rpoD"
                     /locus_tag="ACHURQ_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR0823441.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5654 a         6331 c         5907 g         5345 t
ORIGIN      
        1 ctaatacaaa cactaaaacg ggccatcagg cccgttatat cagtgctcta actccagctc
       61 tcttgcctgc atcatctgtt ggcaatacca ggcataacgt gcgcaaaacc aggttctatg
      121 ttcttcatcc atgttaccag taacggcaag aatatcgacc gggcgacctt ctgcgtgcat
      181 tctggcaaaa ctacgcgcca ggaaatcgaa attatttaaa gaattaagac tatgatccat
      241 aagcacatcc tccggttatc gcataccttt taaggccata tgctttttct gatttcagta
      301 ttccccaatc agtgtagtgc ctttaaacat aacgtgcgta aatatattgt ccccgatcac
      361 actttttagt gaaaaataaa aaactatatt aaaaaacttt ctcttccaga acttctctaa
      421 catgtgttat tattaacaaa gataaatgta agcaaggaaa ctatcccaaa gtaaatattg
      481 cgtcactaaa tggacattgg agtgacatat gattttattc gccgactata atacccctta
      541 tcttttcgct atttcctttg tactgttgat cggcctttta gaaattttag cgcttatctg
      601 cggtcacatg ctctccggtg ctcttgatgc acatcttgac cattataatt ccataaccac
      661 aggtcatatt agccaggcac ttcattatct caatatcgga agattgccag ctctcgtcgt
      721 tctctgcttg ctggctggtt tttttggtct tattggcatc ctgttgcagc atgcctgcat
      781 catggtctgg caatcaccac tgtcgaacct gttcgtggtt cctgtcagtt tactgtttac
      841 gattattgcg gtgcattata ccggtaaaat agttgcgccc tggattccac gcgaccatag
      901 ttcagctatt acagaagaag aatatattgg tagcatggca ttaattaccg gtcatcaagc
      961 cacttcaggt aatccctgcg aaggaaaact caccgatcaa tttggccaaa tacattattt
     1021 actactggag cctgaagaag gaaaaatttt cactaaagga gtcaaagtgt taattatttg
     1081 ccgactttct gcaacgcgat atttggcgga aaacaatccc tggccacaaa tattgtaatt
     1141 tattaatcaa aaggaaattt aataatggat gatattgtta attctgtgcc ctcctggatg
     1201 tttaccgcga ttattgccgt atgcattctg tttattattg gaattatttt cgccaggctc
     1261 tatcgtcgcg cttcggcaga gcaagctttt gttcgtactg gtttaggtgg gcaaaaagtg
     1321 gtgatgagcg gtggcgcaat cgtgatgccg atctttcatg aaataatccc catcaatatg
     1381 aatactctga agctggaagt cagccgctca accattgata gcctgattac gaaagatcgt
     1441 atgcgtgtcg atgtagtagt cgctttcttt gtacgggtaa aaccgtctgt agaaggcatc
     1501 gccactgcgg cgcagacgtt ggggcaacgc acgctatcac cagaagactt acgtatgttg
     1561 gttgaagata aatttgtcga tgccctccgt gcaacagctg cacaaatgac catgcatgag
     1621 ttacaggata cccgcgagaa cttcgtgcag ggagtacaaa atactgtcgc agaagaccta
     1681 tcgaaaaacg gtcttgagct ggaaagcgtt tcacttacca actttaacca gacctcgaaa
     1741 gaacatttca atccgaacaa tgcctttgac gccgaaggtt taaccaaact gactcaggag
     1801 acggagcgcc gtcgtcgcga acgtaacgaa gttgaacagg atgtagaagt tgcggtgcgt
     1861 gagaaaaacc gtgatgcact ttcgcgcaag ttggagattg aacaacaaga agcgtttatg
     1921 acgcttgagc aggagcagca ggttaaaacc cgtactgccg aacagaatgc acgtattgcg
     1981 gcttttgaag ctgaacgtcg tcgtgaagca gagcagacgc gaattctggc tgaacgacaa
     2041 attcaggaaa cggagatcga gcgcgagcag gccgtccgct caagaaaggt tgaggctgaa
     2101 cgtgaagttc gtattaaaga gatcgaacag cagcaggtca ccgaaatcgc caaccagacg
     2161 aaatcgatcg ctattgccgc caaatcggaa caacagtccc aggcagaagc gcgtgctaac
     2221 cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag aaaccactcg ccagaccgca
     2281 gaagccgatc gtgctaaaca agttgcccta atcgctgccg cgcaggatgc agaaaccaaa
     2341 gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag ccgcagagat gcaggcggcg
     2401 gctatcgttg agttagccga agctacacgt aaaaagggtc tggcggaagc agaagcacaa
     2461 cgtgcgctga acgatgctat caacgtactt tctgatgaac aaaccagcct taaattcaaa
     2521 ctggccttgt tgcaggcgct acctgcggta atagagaaat ccgttgagcc gatgaagtcc
     2581 attgacggta tcaagattat tcaggtcgat ggtctgaatc gtggcagcgc tgcgggtgat
     2641 gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac aagcattatc agccgctctc
     2701 tcttaccgca cacaggcacc gctgattgac tccttgctca atgaaattgg cgtttcaggc
     2761 ggctcactgg cggcattgac ttcaccctta acctcaacaa ctcccgtcgc cgaaaacgta
     2821 gaataaattt tcagctatga acgtccctgg cacaaaaagc cggggatttt ttatatctgc
     2881 gttccgctaa aaactgcaaa tgatcaggcc gttgcaacgt tttgcgtgac cgctcgggga
     2941 aggcaaaact gcctctggga aagcgttgcg cgaggtccgg cgctcatcaa caatcgggga
     3001 gtcagcaaag ggctgaaacg ggaaagcccc tcccgaggaa ggggccttac agaaggaaag
     3061 ggttatgatg aagctcgtca tcatactgat tgtgttgtta ctcgtaagtt tcgcagctta
     3121 ttaacagcca atcagagggg ggagaaatcc tccctcaccc ttgttccttt actttaggtt
     3181 gaaaaaaaat cagcgtcaag aaggtttcat taacacatat ccctggccca aaaagccggg
     3241 gattttttat atctgcgttc cgctaaaagg tgcaaatgct caggacgttg cagcgttttg
     3301 cgtgatcgct cggggaaggc aaaactgctt cagggaaagc gttgcgcggg gtccggcgct
     3361 catcaacaat cggggagtca gcaaggggct gaaacgggaa agcccctccc gaggaagggg
     3421 ccttatagaa ggaaagggtt atgatgaagt tcgtcatcat actggttgtg ttgttactgt
     3481 taagtttccc gacttactaa caactgatca gagggggaag aaatcctccc ttacccttgt
     3541 tcctttactt taggttgaaa aaacaacagc gtcaataggc ctgcctgcta caaagcgaga
     3601 tctgtgaacc gctttccggt tagccttttt tatcctgttg gatcttcttg atgatgttag
     3661 tggtcgagca accgtcttcg aagttgagca ccagcacttc gccgccgttg gcccagactt
     3721 ctttactccc ggcgatctct tctggtttat agtcgccgcc cttcaccagt aaatctggca
     3781 ggatcccggc gatcaagcgc tgcggcgtgt cttcttcaaa cgacaccacc cagtcgaccg
     3841 cttccagtgc gcccagcaca atcatacgct gttcgagcgg gttaacgggg cgggaatccc
     3901 ctttcagccg tttggtggag gcatcgctgt tcacagcgac aatcagacgg tcacccagtt
     3961 tgcgagcatt tgccagatag gagacgtgcc cggcgtgcag gatgtcaaag acaccgttgg
     4021 tcattaccac cttttcgccg cgtttacgcg ctgccgctac ggccagcttc agttcctctt
     4081 cggtcatcac gccaaagccg gtatctgcgc gcccacgcac tgcattttcc agctcgatcg
     4141 gcgaaaccgt ggaggttccc agtttgccga ccaccacgcc agccgccgca ttggcaaaga
     4201 agcaggcttc ttccagcgaa ttacccgctg ccagcgttgc cgccaggacg ccaatcaccg
     4261 tgtcgcccgc accggtaacg tcatacactt cctgcgcctg ggttggcata tgcagcggcg
     4321 ctttacccgg ttgcagcagc gacataccct gttcggaacg ggtcactaac agagccgaga
     4381 gttcgtaatc ggcaatcagt ttcatgccgc gctcaacaat ctcttcttcg gtcttacatt
     4441 taccgacaac agcttcaaat tccgagagat tcggcgttaa cagcgtagcg ccgcggtagc
     4501 gctcaaaatc ggtacctttt ggatcaatca gcaccggaac gcccgcttta cgcgccagtt
     4561 ggatcatctg ctgtacgctt gccagcgcac ctttggcgta gtcagaaagc accagcgcgc
     4621 caatcgaact cagcgcctga ttaatccgct cgtgcagcgg ctgcggatca acgccttcga
     4681 aaccttcttc gaaatccaga cggatcagct gttggttgcg ggaaagtacc cgtaatttgg
     4741 tgatggtcgg atgcgtcggt acagaaacga agtcgcattt gacgttgacg tcggccagag
     4801 atttactcag cgcgcgcgct gcatcgtcaa tgcccgtcaa cccgaccagg cgtgcattag
     4861 caccgagaga agcgatattc atcgccacgt tagccgcgcc gcccggacgt tcttcgatgg
     4921 tattcacttt aaccacgggc accggcgctt ccggcgagat acggctggag ggaccgtacc
     4981 agtaacgatc cagcattaca tcaccaacca ccatcactcc tgcacgttca aactctggca
     5041 gcgttacttt cattcctgtc tcctgagaga ttcaaaattt gcgcgcgata ataccatact
     5101 tcattcttcc accagccact tctgccagct tgcccgcacc agatcacgct ctgcggtgaa
     5161 gcaatccccc gacacatggc ccggcaattc ctgtaatgcc agatgatgaa gttcatcgcg
     5221 cagcgtcgtg taagcacggg tcagcgccat cgcttcctgc tcttccataa tgtcgttttg
     5281 cgccagcagc tccagaatac gcacgttgtc tgaccagcgc gttaacttcg gtttttcatg
     5341 agcgtagcgc aacaccagat attgggtaat aaattcgata tcggtaattc cgccttcatc
     5401 agctttgata tcaaagcgat cgcgatgttt attgccgaga tgagcgcgca ttttctcgcg
     5461 catttcccgc acttccgttt gcagagttct accttcacgc ggcagcgtca taatctcgcg
     5521 acgcactgcg tcaaagtgcg cggtgagctg cggatcgccg tacactaccc gcgcacgcac
     5581 cagcgcctga tgttcccacg tccaggcctc gtttttctga taatcggcaa atgcttctgt
     5641 ggaggtaacc agcattcccg ccgccccgga cgggcgcagc cgcgcatcca cttcgtacaa
     5701 aatgccggaa gaggttcgcg tgctgaacag gtgcataatg cgttgcgcca gacgaagata
     5761 aaactgtcgt ccgtcgattt cccgctcgcc gtcagtcatc gcatccattg ggcaatcatg
     5821 gaggaagatc aggtcaagat cggaactgta gcctaactcc cagccgccca gcttgccgta
     5881 gccgaccact gcaaaaccac gcccttcgcg atcgttgagg tgattcggct taccgtagcg
     5941 ggcaaccatt tgaacccacg cctgctgaac gacggcatct atcatggctt ccgccagcca
     6001 ggttaagtga tcgctcactt tcatcaccgg tagcgtacca gcgatatccg ctgcggcgat
     6061 gcgtaacagc tgcgcctgtt tgaactgacg cagcgcctca agctgttgct cttcgtcatc
     6121 ttccggcacg cgcagcaaat actggcgcaa ctcatcgcgg taggcatcgg tcgccgtcgg
     6181 ctggtaaaga gtgtttggat cgagcaattc atccagcaat aatggataac gcgccagctg
     6241 gctggcaatc atcggcgatg cggcacacag agaaatcaaa tggttaagcg ccgcagggaa
     6301 ttcactgagc aattctaaat aggtggtgcg ggtaacaatc cccaccagca aggcggtaat
     6361 gcgcgacagc gtgacagcag cgtcttcacg cgcacagaca tcacttagca gatgcggcat
     6421 cagatggtcg agcacctgac gtcctcgcgg cccgatggtg cgcttatcca gctctttgcg
     6481 gaaatcggaa atcagcgtta gcacctgttt gcgatcatcc tcgctaagat gcgccagcac
     6541 tggcgtagtg tcatcttcct gcaacgcatc ctgccacagc tcacgccact gttccgacag
     6601 cgactcttcc tgagtttcac tttcgtcgtc gccaatcaat tcattaaaca cccggcgcac
     6661 attgttcata tgtgcggtca gcgccccggt cagttgcggc cagtcagcaa agtccatcgc
     6721 ccacgccagc cgcgcacggt taagctcatc agaaggaagc gtctgggttt gttcgtcgtt
     6781 aatgctttgc agcaggtttt ccagacgccg caggaacaga tacgccactc gcaattgttc
     6841 agcatcgttt tcagaaagca gatgcagcgc agcaatggcg ctgagcgttg gcagtaaaga
     6901 gcacgattgc agcgacggtt cgcgcccgcc gcgaatgagc tggaatacct ggacgataaa
     6961 ttcaatttcg cgaataccac ccgcgccaag tttgatgttg tcggtcaaac cgcgccgacg
     7021 cacttcacgg gcaatcattc ctttcatgtt gcgcagcgac tgaatcacgc tgaaatcgat
     7081 gtaacgacgg aaaacaaacg ggcgtagcat cgcacgcaac tcgttagcat agacgccttc
     7141 gctatcgccc ataatccgcg ctttgaccat cgcgtaacgc tcccagtcgc gcccctgctc
     7201 ctggtaataa tcctccaacg cggcaaagct cagcaccagc gggccacttt cgccaaacgg
     7261 acgcagtcgc atatccacgc gatagacgaa gccatcctgc gttggttgat ccagcacttt
     7321 aatcagccgc tgccccatgc gggtaaaaaa ctgcgcgtta tccagttccc ggcgtccgcc
     7381 ctgcgtacaa ccatgttccg gccaggcaaa aatcagatcg atatcagagg agaaattcag
     7441 ttccccgccg cccagcttac ccatgcctaa gatcagcagc ggttgcgctt cgccctgcgc
     7501 attgcacggc gttccccact cgcggcagca ggcgtcatac agccagtcac gcgccgcaac
     7561 aatcagcgtc tccgccagat aactgagctg ctgcaatatg ctctcttcag taaccagtgc
     7621 cagcgtttgc gcccatgcga tgcgcaccat aatgcgccgc cggaatagcc gcagctcgcg
     7681 cattaacccg gcttcgtcac tcacattact gagcgcctcc tgcaaccatg cagcgtaatg
     7741 ctgccattca tcggcctgcg gcggttggct ttccagttcc gtcaaccact ctcgatgcgc
     7801 aattatgctg tcctgcacaa aatcactgaa agtaagtact gacttcgcct gtgcacttag
     7861 tggttcctcg gctaaaggct ctggcagccg ctcaacaacg gtctgccagt actgctgtaa
     7921 cggtgaagag agcggcttca taagcgattt tatccttgcc tgattaacgt tttccgctgt
     7981 gcaaccagaa cggttcctga ttgtttgcct cattacggaa atgctcaatt tcaatgcgtt
     8041 gcccggtcgc aatagcgtga cgcagcccct gccagttctc cagccaggca tgcgcgacga
     8101 caggatcata gtaacccgcc agcaacagta ttgagtcaat gtcacgcgtc aggcgtggca
     8161 actggtcacg gtagagatcg cctaacggct ggcagaaaac gcttttcagt tcagcggcat
     8221 ggtgggaaag atggatatcg gcaaagcgtt tgaaggagtc gctgattttg ccctgcgctt
     8281 tggcatctaa aaatggctgc catgctttgc ttaccaacca ttcggtcaac gccagcttcg
     8341 ccattgccgt ttcggtagag tagaccgccg tcacggcaga aaccgcagaa gcaatggtcg
     8401 cttcgcattg ggtcagcaaa tcacgtaagt gagtgctcgc tttacgcggc acaataccac
     8461 cgaacagcat cagcgtatga cggaccaggc taatggctgc cagcacctgt tctttcgccg
     8521 catcgttacc gcgtacccac agttcttcat gatactgcca ttgcgctaac gccagctcga
     8581 gcgccgcttc cagcccctgt tctacatcgg cttttgccgc aacatgcaaa atggtggtcg
     8641 gtttgatttc acgcgccgga ttgccctgcg ccagatgata gccacgcgcc gctttgctca
     8701 ggctgccctg gcgtaatccg gtttgcgata ccagttggtt cgccagtttc agcaccgcgc
     8761 gcgtgtcgcc gctaagcagt tccagttcca gctcacagat aggttcagca aattcccccg
     8821 ctttcacctc cccctgatcg agagcgattt caattcggct gccatcgacc gccaccagcc
     8881 atttttcgcg ataaaaatcg gtgctgaaca gcggttgtac gcgggaagcg agatcggcag
     8941 gcaattcacc gttcggccag acttccgagg gaaactgcgc caggtcgagc gtcggttcgc
     9001 tcaacgccac gttatattcc gggcgctggt gtaagccgcc tgtcactctt cctgcaactt
     9061 tcatggtcat ctcatagcga ccgttttcgc cacgaatacg taagcccata tcgtgcctac
     9121 gcagccagtt atccggcgtt tcgtagtaaa tattcagcaa ctgcacgggg tcatggtgct
     9181 cgccgcccag cgtattgaga tggtcacgca acgcctcaac ggcactgtga ttaacaataa
     9241 actttaattc gatttcctga gccatggcct ttacttatcg gttatgtcac ataagtgacg
     9301 atgaacggcg aacttaatgc gatctttttt gtcagtagat agtattttgc gccaaattgc
     9361 catgcaacgg gcaatttgac gggcgtaaaa gtttgaagca gtggcaagac ctgacacaga
     9421 ggattccgat tgatgtcgaa tgctttgcgt agcgacactg atgccactac tatcgttcca
     9481 ctttccatga caataacgac agcctgatgc caaaattacg cctgatcgga ttaactttac
     9541 tcgcacttag cgcgactgcc gtctcacacg ctgaagaaac gcgctatgtt tctgacgaac
     9601 tgaatacctg ggtccgtagc ggtccgggag atcattatcg cctcgtgggc acggttaacg
     9661 ccggcgagga agtgacctta ttacaaactg acgccaacac caattatgcc caggtgaaag
     9721 acagctctgg ccgtaccgcc tggatcccgt tgaaacaact tagcactgag ccaagcctgc
     9781 gctcccgtgt gccagatctg gaaaatcagg tcaaaaccct gaccgataaa ctcaccaata
     9841 tcgataacac ctggaatcag cgcaccgcag aaatgcagca aaaagtggcg cagagcgaca
     9901 gcgtgatcaa cgggttaaaa gaagaaaatc agaaactgaa aaacgagctg attgtcgcgc
     9961 agaaaaaggt cgatgccgcc agcgtacagc tggatgacaa acagcgcacc atcatcatgc
    10021 aatggtttat gtatggcggt ggcgtgctgg ggcttggctt gctgctcggt ctggtactgc
    10081 cgcacctgat cccaagccgc aaacgcaaag atcgctggat gaactaaatc gccttcttcg
    10141 ttgcactgtc ttattattag gaattgtgta tttgacgaag ggatgatggc gtgaagattt
    10201 atctggtcgg tggtgctatt cgggatgcat tgttagggct accggtcaaa gacagagatt
    10261 gggtggtagt cggcagtacg ccacaggaga tgctcgacgc gggctaccag caggtaggcc
    10321 gcgattttcc tgtgtttcta catccgcaaa cgcatgaaga gtatgcgctg gcacgtaccg
    10381 aacggaaatc cggttccggt tacaccgatt ttacttgcta tgccgcaccg gatgtcacgc
    10441 tggaagatga tcttaagcgt cgcgatctga ccattaatgc gctggcccag gacgataacg
    10501 gcgagattat cgacccgtac aacggtctgg gcgatctgca aaatcgtctg ttgcgccatg
    10561 tttcccccgc ttttggcgaa gatccgttac gcgtactgcg cgtggcgcgt tttgctgcgc
    10621 gttatgccca cctcggtttt cgtattgccg atgaaactct gacgctgatg cgcgagatga
    10681 cccatgcggg tgaactggaa cacctgacgc ctgaacgggt atggaaagag acggaaagcg
    10741 ccctcaccac ccgcaatcca caggtgttct tccaggtact gcgcgattgc ggcgcactgc
    10801 gcgttttatt cccggaaatt gacgcactgt ttggcgttcc ggcccctgcc aggtggcatc
    10861 cggaaatcga cacgggtatt cataccttaa tgacgctctc aatggcggcg atgctgagtc
    10921 cgcaggtcga tgtccgtttc gcaactttat gccacgatct cggtaaagga ctgacgccgc
    10981 cagaactttg gccgcgtcat catggtcacg gtccggcggg tgtgaaatta gtggaacaac
    11041 tctgccagcg tctgcgcgtg ccaaatgaaa ttcgcgattt agccagactg gtggctgagt
    11101 ttcacgatct catccacacc ttcccaatgc tgaacccaaa aactatcgtc aaattgtttg
    11161 attccatcga cgcctggcgt aaaccgcagc gtgtcgagca actggcgctg accagcgagg
    11221 ctgacgtacg cggcagaacc ggttttgaat cagcggacta cccgcaaggc cgttggttgc
    11281 gcgaagcctg ggaagtggcg cagtcagtgc cgacaaaagc ggtcgttgaa gcgggattta
    11341 aaggtgtgga gattcgcgag gagctgaccc gacggcggat tgcggcggta gccagttgga
    11401 aggaacaacg ttgcccaaag cctgactgag tataaaacta aacgttaaca cgaaccatcc
    11461 cctcgcccct tcggggagag ggttagggtg aggggaacag accagcaccg gtgcgaacaa
    11521 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    11581 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    11641 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    11701 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg agatatcgcc ggttgtcagg
    11761 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    11821 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    11881 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    11941 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    12001 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    12061 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    12121 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    12181 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    12241 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    12301 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    12361 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    12421 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    12481 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    12541 attacaccaa cattcgccgc ccgcgccatt gcgcctggct tgctgagttt gatgcgcacc
    12601 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    12661 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    12721 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    12781 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    12841 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    12901 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    12961 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    13021 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    13081 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    13141 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    13201 attaggtgtc agccccttct ggttaggctt aattgccatc gccgcctgtc ttggacacat
    13261 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    13321 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    13381 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    13441 ctggtggttt aagccacaat tcaccttccc ggtttcgatg ctctcttgcc tgatcctgct
    13501 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    13561 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    13621 agccttcatc gggcttgccc agccgttgct ggcaccatgc cgccagaaat tccacgcaga
    13681 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    13741 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    13801 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    13861 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    13921 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    13981 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    14041 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    14101 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    14161 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    14221 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    14281 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    14341 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    14401 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtctgccca agcgtcgccg
    14461 ctgcggcgct aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    14521 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    14581 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    14641 aattgttaat tacatattca cggtggcaaa aaatataaaa ccacattttt agtggtagtt
    14701 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    14761 aataagcaac aggcagtgaa taagttgacg gagattgtcg ctaactttac cgccatgatt
    14821 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    14881 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    14941 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    15001 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    15061 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    15121 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    15181 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    15241 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    15301 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    15361 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    15421 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    15481 caagggctga ctggcaacag ttcagtgatg ggcgtacata tcgaatctgc cgcccgccat
    15541 ccgtcaacca tcggcgttgc tgtctctacc ggctgctggg cgcatcgtcg cggcacactg
    15601 ctggttcatg ccgatctcac ctttgaaaat ctgtctcaca cccggagcgc gttatgaaaa
    15661 agatcctgac aaccccgatc aaagctgaag atctgcaaga tattcgcgtc gacgatgtga
    15721 tctacctgac cggtacgctg gtgacctgcc gtgacgtttg tcaccgccgc ttgatcgaac
    15781 tgaaacgtcc gatcccttac gatctcaacg gcaaagcgat tttccacgct ggccccatcg
    15841 tgcgcaaaaa cggcgataaa tgggagatgg tttccgttgg cccgacaacc agtatgcgta
    15901 tggaaagttt tgaacgtgaa tttatcgagc agaccggcgt gaaactggtg gttggcaaag
    15961 gcggaatggg gccgctgacc gaagaaggct gccagaaatt caaggcgcta catgtgattt
    16021 tcccggcagg ctgcgcggtg ctggcggcaa cccaggtgga agagattgaa gaagtgcact
    16081 ggacagagct cggaatgccg gagtcgctgt gggtctgtcg ggtcaaagag ttcggcccgc
    16141 tgattgtctc tattgatacc cacggcaaca acctgatagc cgaaaacaaa aagctgttcg
    16201 ccgaacgccg cgatcccatc gtggaagaga tctgcgagca cgtccattac atcaaataac
    16261 cctcccggag aggctcgccc cctctccttt ttcgcaggca taacacgatg aaaccttcca
    16321 ctgaatggtg gcgatacctt gcgccgctgg cggtcatcgc cattattgct ctaattccgg
    16381 ttcccgcagg gctggagagt catacctggc tctactttgc cgtttttact ggcgtgatcg
    16441 ttggactgat cctcgaaccc gtgccgggtg ccgtggtggc gatggtgggt atatccatca
    16501 tcgccatact ctctccctgg ctgctgttca gcccggagca gctcgctcag ccaggcttta
    16561 aattcactgc aaaatccctc tcgtgggccg tttccggttt ttctaattcg gttatctggc
    16621 tgattttcgc cgcctttatg tttggcacag gctatgaaaa aaccgggctt ggacgccgca
    16681 ttgcgctgat tctggtgaaa aagatgggac atcgcacgct gtttctcggc tatgcggtga
    16741 tgttctccga gctgatcctc gcacctgtaa caccgtccaa ctcggcgcgt ggtgcgggga
    16801 ttatctatcc catcatccgt aacctgccac cgctctatca atcacaacca aacgacagca
    16861 gttcgcgcag cattggctcg tacatcatgt ggatggggat tgttgccgac tgcgtgacca
    16921 gcgccatttt cttgacggcg atggcaccaa acttgctgtt aattggactg atgaaaagcg
    16981 catctcacgc cacgctgagt tggggcgact ggttcctcgg gatgttgccg ctcagtattt
    17041 tgctggtgct gctggttccc tggctggctt atgtgctgta cccgccggta ctgaagtctg
    17101 gcgatcaggt gccgcgctgg gcagagacgg aactgcaggc aatgggcccg ctctgttcgc
    17161 gtgaaaaacg gatgctgggg ctgatagtag gcgcgctggt gctgtggatt ttcggcggtg
    17221 attatatcga tgccgcgatg gtcggttaca gcgtggtggc actgatgctg cttctgcgca
    17281 ttatcagttg ggacgacatt gtcagtaata aagcggcgtg gaacgttttc ttctggctgg
    17341 cctcgcttat caccctcgct accggactca acaacaccgg ttttattagc tggtttggca
    17401 aactgttagc aggcagctta agcggttatt cgccaacgat ggtgatggtg gcgttgattg
    17461 tggtgtttta tctgctgcgc tactttttcg ccagcgccac ggcgtatacc tccgccctcg
    17521 caccgatgat gattgccgct gcgctggcga tgccggaaat cccgctgccg gtgttctgcc
    17581 tgatggttgg cgcggcaatt ggtctgggga gcattcttac accatacgcc accggaccca
    17641 gtccgattta ctacggtagt ggttatctgc caacggcgga ttactggcga ctgggggcga
    17701 tttttgggct gatattcctc gtgttgctgg tgattaccgg cttactgtgg atgcccgtgg
    17761 tgttgcttta agtgaggacg gtgccggaaa ttgctctccg gcacccaata gttttacgca
    17821 gccggtaact ccgccagcgg ccagcgcgga cgcacgctaa cgccgagatc cgccgtcgcg
    17881 cctgctttaa accgcaccat tccggcatag gcgatcatcg cgccgttatc agtacaaaac
    17941 tccggacgcg cgtagaacac ttcgccgcgg cgttttttca tcatttcagc cagcttcgcc
    18001 cgtaacgtgc ggttagcact cacgccgccc gccatgacca gtcgcttaaa gcccgtctga
    18061 tccaacgctc gcttacactt aatcatcaac gtatcgacca ccgcatcttc aaaggcgcgg
    18121 gcgatatcag cacgcgtctg gtcgtcggtg ccgttgtcac gaatcgtgtt cgccgcaaag
    18181 gttttcagac cagaaaagct gaaatccagc cccggacggt cggtcatcgg acgcgggaaa
    18241 acaaagcgcc cggcagtacc ctgagccgcc attttcgaca gtaacggtcc gccaggataa
    18301 tccagcccca gcagcttcgc ggttttatca aacgcttcac cggcggcatc atcgatagac
    18361 tcgccgagca gctcgtactg accaatgcca gtcacgctga ttaactgcgt atgaccgccg
    18421 gacaccagca gcgcgacaaa cggaaattcc ggcgggttat cttccagcat cggcgctaac
    18481 agatgccctt ccatatggtg taccgggatt gccggaacgt tccaggcaaa cgccagagaa
    18541 cgccccacgg ttgcgccaac cagcagtgcg ccgactaatc cagggcctgc ggtataggcc
    18601 acagcatcaa tgtcttttgc cgttaagcca gactccttta gcgccgcctg gatcaacggt
    18661 acggttttgc gcacgtgatc gcgggaggcc agttcaggca cgacgccgcc atagtcagcg
    18721 tgcaatttca cctgactata caattggttg gctaacaaac ctttttcatc gtcgtaaatg
    18781 gcgatgccgg tttcatcgca ggaagtttca atacccagta cacgcatgac ttgttttacc
    18841 tcgctttatt accgcgcagt gtaggaccaa tgcgggttga tgtaaaactt tgttcgcccc
    18901 tggagaaagc ctcgtgtata ctcctcaccc ttataaaagt ccctttcaaa aaggccgcgg
    18961 tgctttacaa agcagcagca attgcagtaa aattccgcac cattttgaaa taagctggcg
    19021 ttgatgccag cggcaaaccg aattaatcaa aggtgagagg cacatgccgg taattaaagt
    19081 acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc aagcgttcct gcgaaaaagc
    19141 aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa aaaccgacta ccgaacgtaa
    19201 gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa ctggctcgcg aaaacgcacg
    19261 ccgcactcgt ctgtactaat tccccgagag cgttgctctc cgatcagacc gagttgtagt
    19321 tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt cgtttatgaa ttgctaaaaa
    19381 tcggggccta tggctggacg aatcccacgc gtattcatta atgatctgct ggcacgcact
    19441 gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa agcagggcaa gaatttccac
    19501 gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca ccgttaacgg tgagaaacag
    19561 ttttaccact gctttggatg tggcgcgcac ggcaacgcga tcgacttcct gatgaactac
    19621 gacaagctcg agttcgttga aacggtcgaa gagctggcag caatgcacaa tcttgaagtg
    19681 ccatttgaag caggcagcgg ccccagccag atcgagcgcc atcagaggca aacgctttat
    19741 cagttgatgg acggtctgaa tacgttttac caacaatctt tacaacaacc tgttgccacg
    19801 tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg aggttatcgc tcgctttgcg
    19861 attggttttg cgccccccgg ctgggacaac gtcctgaagc ggtttggcgg caatccagaa
    19921 aatcgccagt cattgattga tgcggggatg ttggtcacta acgatcaggg acgcagttac
    19981 gatcgtttcc gcgagcgggt gatgttcccc attcgcgata aacgcggtcg ggtgattggt
    20041 tttggcgggc gcgtgctggg caacgatacc cccaaatacc tgaactcgcc ggaaacagac
    20101 attttccata aaggccgcca gctttacggt ctttatgaag cgcagcagga taacgctgaa
    20161 cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg tggcgctggc gcaatacggc
    20221 attaattacg ccgttgcgtc gttaggtacg tcaaccaccg ccgatcacat acaactgttg
    20281 ttccgcgcga ccaacaatgt catttgctgt tatgacggcg accgtgcagg ccgcgatgcc
    20341 gcatggcgag cgctggaaac ggcgctgcct tacatgacag acggccgtca gctacgcttt
    20401 atgtttttgc cggatggcga agaccctgac acgctggtac gaaaagaagg taaagaagcg
    20461 tttgaagcgc ggatggagca ggcgatgccg ctttccgcat ttctgtttaa cagcctgatg
    20521 ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt tgagtacgct ggcactgcca
    20581 ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc ttcgtcagga attaggcaac
    20641 aaattaggca tacttgatga cagccagctt gaacgattaa tgccaaaagc ggcagagagc
    20701 ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca tgcgtatact tatagggttg
    20761 ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc ttgagaatct ggatgaaaat
    20821 aagctccctg gacttggctt attcagagaa ctggtcaaca cttgtctctc ccagccaggt
    20881 ctgaccaccg ggcaactttt agagcactat cgtggtacaa ataatgctgc cacccttgaa
    20941 aaactgtcga tgtgggacga tatagcagat aagaatattg ctgagcaaac cttcaccgac
    21001 tcactcaacc atatgtttga ttcgctgctt gaactgcgcc aggaagagtt aatcgctcgt
    21061 gagcgcacgc atggtttaag caacgaagaa cgcctggagc tctggacatt aaaccaggag
    21121 ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc gatcgggaag cccccgacag
    21181 ccgcactgag aggcagcggc aaatatataa gtacgccctc gtaattatcg ttggcggtaa
    21241 acaaccgttg gatttcagcg ttaacggctg aaggacatcg ggtcaatcgc ccaacaccaa
    21301 cctcatgaaa taagtgtgga taccgtctta tggagcaaaa cccgcagtca cagctgaaac
    21361 ttcttgtcac ccgtggtaag gagcaaggct atctgaccta tgccgaggtc aatgaccatc
    21421 tgccggaaga tatcgtcgat tcagatcaga tcgaagacat catccaaatg atcaacgaca
    21481 tgggcattca ggtgatggaa gaagcaccgg atgccgatga tctgatgctg gctgaaaaca
    21541 ccgcggacga agatgctgcc gaagccgccg cgcaggtgct ttccagcgtg gaatctgaaa
    21601 tcgggcgcac gactgacccg gtacgcatgt acatgcgtga aatgggcacc gttgaactgt
    21661 tgacccgcga aggcgaaatt gacatcgcta agcgtattga agacgggatc aaccaggttc
    21721 aatgctccgt tgctgaatat ccggaagcga tcacctatct gctggaacag tacgatcgtg
    21781 ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg ctttgttgac ccgaacgcag
    21841 aagaagatct ggcacctacc gccactcacg tcggttctga gctttcccag gaagatctgg
    21901 acgatgacga agatgaagac gaagaagatg gcgatgacga cagcgccgat gatgacaaca
    21961 gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact acgcgctcag tacgttgtaa
    22021 cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc cgctcaggaa gagatcctga
    22081 aactgtctga agtattcaaa cagttccgcc tggtgccgaa gcagtttgac tacctggtca
    22141 acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga acgtctgatc atgaagctct
    22201 gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac cctgtttacc ggcaacgaaa
    22261 ccagcgatac ctggttcaac gcggcaattg cgatgaacaa gccgtggtcg gaaaaactgc
    22321 acgatgtctc tgaagaagtg catcgcgccc tgcagaaact gcagcagatt gaagaagaaa
    22381 ccggcctgac catcgagcag gttaaagata tcaaccgtcg tatgtccatc ggtgaagcga
    22441 aagcccgccg tgcgaagaaa gagatggttg aagcgaactt acgtctggtt atttctatcg
    22501 ccaagaaata caccaaccgt ggcttgcagt tccttgacct gattcaggaa ggcaacatcg
    22561 gtctgatgaa agcggttgat aaattcgaat accgccgtgg ttacaagttc tccacctacg
    22621 caacctggtg gatccgtcag gcgatcaccc gttctatcgc ggatcaggcg cgcaccatcc
    22681 gtattccggt gcatatgatt gagactatca acaaactcaa ccgtatttct cgccagatgc
    22741 tgcaagagat gggccgtgaa ccgacgccgg aagaactggc tgaacgtatg ctgatgccgg
    22801 aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc aatctccatg gaaacgccga
    22861 tcggtgatga tgaagattcg catctggggg atttcatcga ggataccacc cttgagctgc
    22921 cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac gcacgacgtg ctggctggcc
    22981 tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg tatcgatatg aacaccgacc
    23041 acacgctgga agaagtgggt aaacagttcg acgttacccg cgaacgtatc cgtcagatcg
    23101 aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc tgaagtgttg cgtagcttcc
    23161 tggacgatta atcggtaggc cggatcaggc gttacgccgc acccggcact agtccctctg
    23221 cacaaacgcc acctttt
//
LOCUS       DAZOZY010000048        22946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZOZY010000048 DAZOZY010000000
VERSION     DAZOZY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.048"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(206..685)
                     /gene="folK"
                     /locus_tag="ACHURQ_001636"
     CDS             complement(206..685)
                     /gene="folK"
                     /locus_tag="ACHURQ_001636"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR0823442.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(682..2079)
                     /gene="pcnB"
                     /locus_tag="ACHURQ_001637"
     CDS             complement(682..2079)
                     /gene="pcnB"
                     /locus_tag="ACHURQ_001637"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR0823443.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2139..3035)
                     /gene="gluQRS"
                     /locus_tag="ACHURQ_001638"
     CDS             complement(2139..3035)
                     /gene="gluQRS"
                     /locus_tag="ACHURQ_001638"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR0823444.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(3102..3557)
                     /gene="dksA"
                     /locus_tag="ACHURQ_001639"
     CDS             complement(3102..3557)
                     /gene="dksA"
                     /locus_tag="ACHURQ_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR0823445.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3735..4439)
                     /gene="sfsA"
                     /locus_tag="ACHURQ_001640"
     CDS             complement(3735..4439)
                     /gene="sfsA"
                     /locus_tag="ACHURQ_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR0823446.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4454..4984)
                     /gene="thpR"
                     /locus_tag="ACHURQ_001641"
     CDS             complement(4454..4984)
                     /gene="thpR"
                     /locus_tag="ACHURQ_001641"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR0823447.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5058..7487
                     /gene="hrpB"
                     /locus_tag="ACHURQ_001642"
     CDS             5058..7487
                     /gene="hrpB"
                     /locus_tag="ACHURQ_001642"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR0823448.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7684..10218
                     /gene="mrcB"
                     /locus_tag="ACHURQ_001643"
     CDS             7684..10218
                     /gene="mrcB"
                     /locus_tag="ACHURQ_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR0823449.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10438..12627
                     /gene="fhuA"
                     /locus_tag="ACHURQ_001644"
     CDS             10438..12627
                     /gene="fhuA"
                     /locus_tag="ACHURQ_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR0823450.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12678..13475
                     /gene="fhuC"
                     /locus_tag="ACHURQ_001645"
     CDS             12678..13475
                     /gene="fhuC"
                     /locus_tag="ACHURQ_001645"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR0823451.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13475..14365
                     /gene="fhuD"
                     /locus_tag="ACHURQ_001646"
     CDS             13475..14365
                     /gene="fhuD"
                     /locus_tag="ACHURQ_001646"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR0823452.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14362..16323
                     /gene="fhuB"
                     /locus_tag="ACHURQ_001647"
     CDS             14362..16323
                     /gene="fhuB"
                     /locus_tag="ACHURQ_001647"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR0823453.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGVLFQQVLRNPLAEPTTLGVATGAQLGITVTTLW
                     AIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAINQLLVIF
                     HHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLDDGVARNL
                     GLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPRLMLASLI
                     GALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDMKVNDRVA
                     AERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWPRIMAALF
                     AGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWLLPAGSIG
                     AAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQVLTWISGS
                     TYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTRIALLLLA
                     ACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFADWCGRMV
                     LFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16481..17761)
                     /gene="hemL"
                     /locus_tag="ACHURQ_001648"
     CDS             complement(16481..17761)
                     /gene="hemL"
                     /locus_tag="ACHURQ_001648"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR0823454.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17927..17974
                     /gene="yadX"
                     /locus_tag="ACHURQ_001649"
     CDS             17927..17974
                     /gene="yadX"
                     /locus_tag="ACHURQ_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR0823455.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17986..19407
                     /gene="clcA"
                     /locus_tag="ACHURQ_001650"
     CDS             17986..19407
                     /gene="clcA"
                     /locus_tag="ACHURQ_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR0823456.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19431..19496
                     /gene="yadW"
                     /locus_tag="ACHURQ_001651"
     CDS             19431..19496
                     /gene="yadW"
                     /locus_tag="ACHURQ_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR0823457.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19489..19833
                     /gene="erpA"
                     /locus_tag="ACHURQ_001652"
     CDS             19489..19833
                     /gene="erpA"
                     /locus_tag="ACHURQ_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR0823458.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19880..20503)
                     /locus_tag="ACHURQ_001653"
     CDS             complement(19880..20503)
                     /locus_tag="ACHURQ_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR0823459.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20541..21341)
                     /gene="btuF"
                     /locus_tag="ACHURQ_001654"
     CDS             complement(20541..21341)
                     /gene="btuF"
                     /locus_tag="ACHURQ_001654"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR0823460.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21334..22032)
                     /gene="mtnN"
                     /locus_tag="ACHURQ_001655"
     CDS             complement(21334..22032)
                     /gene="mtnN"
                     /locus_tag="ACHURQ_001655"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR0823461.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22116..>22946
                     /gene="dgt"
                     /locus_tag="ACHURQ_001656"
     CDS             22116..>22946
                     /gene="dgt"
                     /locus_tag="ACHURQ_001656"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0823462.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
BASE COUNT         5227 a         5810 c         6383 g         5526 t
ORIGIN      
        1 ttttgaacaa attaaatttt cttaattcaa aattaagcaa aaataaattc acaagtgcaa
       61 ttggttaggg tatggcgatg ggattgatat ttatctgcgg gaatttatat tatgtttctg
      121 caaaaatgtt taaactaaaa tttttattaa ggaaagagag gatgtatgat tttcagaaaa
      181 tatttatatt cgtaatataa ataaattacc atttgcttaa ttcgtcaaat gctcttgtat
      241 gtaagacttc acgcaacgtc tccccatcag gaaacgccag ctccggtgca atctcaaaca
      301 gcggccacag cataaatcca cgattcttca tatcgtagtg cggaacggtc aggcgttcgg
      361 tatttatcac ttcattacca aacagcatga tatcgagatc cagcgtgcgt ggtccccagc
      421 gttcagcttt acgaacacga ccctgttgca gttctatgcg ctgtgtatgg ttgagtaact
      481 cttccggggc gagcgaagtt tcaagcgcca cggcggcgtt gaggtaatcg ggttgatctt
      541 gcggccccag cggtggagtg cggtaaaacg aagaaacggc aagaatgtgg ctttcaggga
      601 tatcgcctaa tgctttcagg gcagcattga cctgctccag cggagaggcc agattgctgc
      661 ctatggcaat atacgccact gtcatgcggt accctcacga cgcggtgcgc gtttgcgtgg
      721 acgacgagta cggcgacgcg gtgacggctc ttcatccaac tcgttaagca tccctttttg
      781 atctggtggc gcggaaacct ggaactcacc ccaccatttc accagacgct gcagttcagc
      841 gttacgttca acttcagctc gcaaggccaa caggtcataa gccgcacgga acttaggatg
      901 ctccagcagt ttccatgcgc gtttaccctg acgtcgggac atacgcaact gcaactgcca
      961 gatatcgcgg gttaacgtcg tcaggcgttt cgggattgcc agtgaacggc aggcttcgtc
     1021 cagcacgtcg ttcatcgcca gcgcgaaagc gtcgtgatag gtcaggccgc tttcctgggc
     1081 gatcttctgt gccgtctcca gcagtgggta ccagaacatg gcggcaaaca agaacgccgg
     1141 gttcacgcgc atatcgttat ggatacgcgt atcggtattc ttcagcacct gttcaatgat
     1201 ccgctccatc gggctgtcgc cattttccgt gaagtagcgg gtaatggtcg ggaacagcgg
     1261 ctggaacaga tgatattcac acaacagctt ataggtttcg taaccgtagc ccgcttgtag
     1321 cagtttaagc gattcttcaa acaggcgtgc cggtgggata tcgttcagca gggtagcgag
     1381 gcgagggatc ggttctgcgg tttccgggct gatgcgcata cccaatttgg cggcaaaacg
     1441 taccgcgcgc agcatccgta ccggatcttc acggtagcgc gtttccgggt taccaatcag
     1501 acggataacg ccgtccttca gatccttcat accgccaacg taatcacgga cggtaaaatc
     1561 cgctacgctg taatagaggc tgttgatagt gaaatcgcgg cgctgggcgt cttcttcgat
     1621 ggagccgaaa atgttgtcgc gcagcaacat gccgttttgc ccgcgttggg aggtcgtgcg
     1681 gtcgctgacg ttaccttcgt ggtgtccacg gaaggtcgca acttcgataa tctccgggcc
     1741 aaacattaca tgagccagac ggaaacggcg acccaccagg cggcagttac ggaacagttt
     1801 gcgcacctgc tcaggcgtgg cgttagtggt tacgtcaaaa tctttcggct ttttgccaag
     1861 taacaggtcg cgcacgccgc cgccaaccag ccaggcttcg tatcccgctt tgttgagcct
     1921 gtacattacc ttcagggcat tttcactgat atctttgcgg gaaatagcat gctgctcacg
     1981 cgggatcacc gtcacctgtg gacgggcgac tgcctgttca gcctcgcttt cctcgcggct
     2041 tagcaccttg cggcaaaaat tagcgactcg ggtaaaaata gtacacctcg gtagtgtcaa
     2101 acatcattca ggacaaaaaa atagcggcta atcatagctc agcatgacgc atttgagaat
     2161 gttgaattta caattgccga ctcgggcacg gcggtcagcg tccagttttt gactgctgac
     2221 tgaaggattt gttcgacgct gaaatcctgc cagtgtgctt ctgcctgctg acccagaaat
     2281 tgaagtgccg cgattagtac cggacgtgga tcgcctttcg gcaacgcagg cgcatgattc
     2341 tgcttggaaa gtttagcgcc ttgtggatta agcgccagcg gcagatgaat gtaatctggc
     2401 actttccagc caaaaagctg gtacagcgag atttgcctta ctgttggttc aatcagatca
     2461 gccccacgca ctatttctgt aacgccctgg aaatgatcat caaccacaac agccaggttg
     2521 taggcgaaca acccatcacg gcgatgaatg ataaaatctt cccgtgccag tttttcgtcg
     2581 gcgtgaataa tgccgcgcag ctggtcagta aattgcgtga ccggatgctg ctggcggata
     2641 cgcactgcgg cattgtctgg tccatgatgc aacacccggc aatgaccgtc gtaaataccg
     2701 ccaatgcttt gaatacgcgc acgcgtacag gtgcagtaat aacttagtcc ttgttcatgt
     2761 aaccaggcga gtgcttcacg ataggcgtcg tgacgttgcg attgccagag aacatcgccg
     2821 tcccagtgca gaccgtaatg ttccagctgg cgcaggatag tttctgcggc accgggaact
     2881 tcacgaggcg ggtcgatatc ttctatgcgt accagccagc gaccttgccg ggcgcgagcc
     2941 tgcaaatagc tgccgagcgc ggcgatcaga gagccaaaat gaagctcgcc ggaaggagag
     3001 ggggcgaagc ggccaatata ctgtgtgtct gtcatctctt tgaacaaaaa ataaggcggg
     3061 agcatttccc gcctgtggta aacgtgatgg aacggctgta attagccagc catctgtttt
     3121 tcgcgaattt cagccagcgt tttgcagtcg atgcacagat cggctgtcgg gcgcgcttcc
     3181 agacggcgaa taccaatttc aacaccgcag gattcgcagt agccgaaatc ttcgtcttcc
     3241 acttttttca gcgtcttctc gatctttttg atcagcttac gctcgcgatc gcggttacgc
     3301 agttcgaggc tgaactcttc ttcctgggct gcacggtcta ccgggtccgg gaagttggct
     3361 gcttcatcct gcatatgtgt aacggtgcga tcgacttcat ccctgagttg attacgccat
     3421 gcttccagaa tacgacggaa gtgcgccagc tgggcttcat tcatatactc ttcgcccggc
     3481 ttctcctgat atggttccac cccagcgatg gcgagaatac tcagggacga tgttttacgg
     3541 ttttgccctt cttgcatgtt gcttctcctt aacacgcact atcgatcccc atgttcgggg
     3601 gaaaaatgag gccgctataa atagcagatg cttttccgga tagcaattat ctaaacgtaa
     3661 cacttgacaa ctgtgtgagg aaaagcgtat ttgcgcacgc gaccagaatg taaattaacc
     3721 agttacttac tttactacaa tgtaaccgac agtgattttt taagagccat gccttcagca
     3781 gaaatttccg ctttgtaagc cagaatttct acccccctct gttgagcttc tgacaatagt
     3841 tgcgcgtatt tctcatcgat gtggcgcgcg ggtgaaaacc gtgtaatggc tgaatgcagc
     3901 acggcgaaaa agataaccgc acgctggcct tcagccgcta cgttcatcaa ctcccgaagg
     3961 tgtttctgac ctcgttcagt gaccgcatcg ggaaaatatc cctgttcgtt ctccgctaac
     4021 gtaaccgatt tcacttcaat atagcagtct ggacgcgaat ccgcctgcaa cataaagtca
     4081 atacggctgc gttctgcgcc gtattttact tcgcttttca gcgagctata gcctgacagt
     4141 tctgaaattg attcattaag gatagcctct ttcgtcaacc tgttagccca aagcgtattg
     4201 acgcaaataa atgcgccgct ctggctttga gttagttccc aggtgtgtgg gtatttccgt
     4261 ttggtgttgt ctgaagtcga ataccagacg gtatcgccac gcgttgcaca accggtcatc
     4321 gcacccgtat tcgggcagtg tagcgttaat tcgcgaccat cgggtgtgat cacatcggct
     4381 aaaaaacgtt tgtaacgctg aattagcgtc gcgcgctgta gagggggaga gaattccatt
     4441 gcgacaatcc ttgttattgc gttagcgccc agcgttttag cggcgtgtag cgtgtgcgtc
     4501 cacgggcaaa cgaggaggcg taaagggtga actccgtcac cgcataagac cagttaaaac
     4561 ctggcggcgg gattgtcacc gcctcgctgg cgtcgcgcaa taaggtaata tgtggatgaa
     4621 acggacgatt gctttgaaaa caaccgctgc gggcagcctg tgaacggagc atattcgcca
     4681 gctggattaa gccgcgtggc ggctgacgca tccctaacca caccacacgc gaacgcagcc
     4741 attgtccggc gtcatcaagc gtgagtgtga aaccaggttg acgaatccgt ccggctaaaa
     4801 gagaaagcgc cttctctttc tcggcgctca cttcgcctaa aaatgccaga gtcagatgca
     4861 aattatcggc ggcgaccgga cgtcccgcct caggtgggaa gtgtgtggcg cgccagtgga
     4921 taatctgttc gcggatttct gcaggtaagt cgatagcaaa gaacagacgt tgcggttcag
     4981 acatgtgagg cactcggtta tgaattaccg cgatgctaca atgtggcgcg aagaatgtta
     5041 accctctgga gcgttttgtg tcgtcgttgc ccgttgctgc cgtcttacct gaattactta
     5101 ccgctctcga ttgtgcgccg caggtattat taagtgcgcc gaccggggcc gggaaatcaa
     5161 cctggctgcc gctgcaactg ctggcgcatc ccggcattaa cgggaaaatt atcctgctgg
     5221 agccgcgtcg tctggcggcg cgtaacgtcg cgcaacggct ggcggagctg cttaacgaaa
     5281 agccaggcga taccgttggc taccggatgc gtgcgcaaaa ctgcgtcggg ccaaataccc
     5341 gtctggaagt agttaccgaa ggcgtgctga cgcggatgat ccagcgtgac ccggagttga
     5401 gcggtgttgg attggtgatc cttgatgaat ttcatgagcg cagcttgcag gcggatttgg
     5461 cgttggcgct gttactcgat gtgcaacaag gtctgcgtga tgaccttaaa ctgctgatta
     5521 tgtcggctac gctggataac gaccgcttgc agcaaatgct gccagaagcg cccgtcgtca
     5581 tctcagaagg gcgctcgttt ccggttgaac gccattattt accgctgccc gcgcatcagc
     5641 gttttgacga agccgttgcg gtagccaccg ccgaaatgct gcgtcaggaa agcggatcat
     5701 tactgttatt tttacctggc gtcggagaaa ttcagcgtgt gcaggaacaa ctggcttcgc
     5761 gcatcggcag tgatgtgttg ctctgcccgc tgtatggcgc gttgtcgctg aacgatcagc
     5821 gaaaagcgat cctcccggca ccgcaaggga tgcgcaaagt ggtgctggcg accaatattg
     5881 ctgaaaccag tttaaccatt gaaggtattc gtctggtggt ggattgtgcc caggagcgtg
     5941 tggcgcgttt tgagccgcgc acggggctta cgcgactgat tactcaacgc gttagccagg
     6001 catccatgac gcagcgtgcc gggcgcgccg ggcgtctgga gccgggtatc tgcctgcatt
     6061 taatcgccaa agaacaagca gaacgcgctg ccgcgcaaag tgaaccggag atcttacaaa
     6121 gcgatctttc cggtttgctg atggaattac tgcaatgggg atgcagcgat ccggcgcaga
     6181 tgagctggct ggatcaaccg ccagtagtga atctaatggc cgcgaaacgt ctgttacaaa
     6241 tgctgggggc gctggacggt gaacggctta gtgcgcaagg gcaaaaaatg gcagcgctgg
     6301 gtaacgatcc gcgtttagcg gcaatgctgg ttagcgcgaa gaacgacgac gaagctgcta
     6361 ccgcggcaaa aattgccgcc attctcgaag agccgccact gatgggcaat agtgacctgg
     6421 gcgtggcgtt ttcgcgcaat caaccagcct ggcagcaacg tagtcagcaa ctgttaaaac
     6481 gcttaaacgt acgcggcggt gaggcagaca gttcgcttat cgcgccgcta cttgccgggg
     6541 cgtttgccga tcgcattgct catcgccgtg ggcaagatgg acgctatcaa ctggcaaacg
     6601 gcatgggagc gatgctcgat gccgacgacg cgctaagccg ccacgaatgg ttgatcgcac
     6661 cgttattatt gcagggcagc gcctcgccgg atgcgcggat tttactggcg ctaccggtcg
     6721 atattgatga attagtacaa cgctgtccgc agctggtgca gcaatctgac accgtggaat
     6781 gggatgacgc gcaaggtacg ctgaaagcct ggcgtcggct acaaatcggt cagttgacgg
     6841 tgaaagtgca gccgctggcg aaaccgtcag aagacgagtt gcatcaggcg atgcttaatg
     6901 gcatccgtga taaaggttta agcgtgctca actggacggc ggaagcggaa cagctacgct
     6961 tgcgtttgtt atgcgccgca aagtggttgc cggaatatga ctggccagcg gttgatgatg
     7021 aaagtttatt ggcagcgctg gaaacgtggc tgctgccaca tatgacaggc gtacattcac
     7081 tacgcggcct gaaatcactg gacatttatc aggcactacg cggattactt gattggggaa
     7141 tgcagcaacg tctggatagt gaattgcctg cgcattacac tgtgccgacg ggaagccgga
     7201 tcgccattcg ttatcatgaa gataacccgc ccgcactggc ggtgagaatg caggagatgt
     7261 ttggtgaggc cactaatccg actatcgccc aggggggcgt gccgctggtg ctggagttgc
     7321 tttcacccgc ccaaaggcca ctacaaatca cacgagattt gagcgccttc tggaaaggag
     7381 cgtaccgtga ggtgcaaaaa gagatgaaag ggcgttatcc caaacacgtc tggccggacg
     7441 acccggcaaa cacggcaccg acgcgacgga cgaaaaagta ttcgtaaggt tggttttctc
     7501 cctctccctg tgggagaggg ccggggtgag ggcatcagcg tgcgcatttc cccctcaccc
     7561 taaccctctc cctcaaggga gaggggaccg atcgagcaca aattttgaga gatatcttct
     7621 tctgtcttgt aacagaagaa cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa
     7681 agcatggccg ggaatgaccg cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc
     7741 aaacaaaagg taagccgtcg tcgttacgaa gatgacgatg attacgacga ttatgatgac
     7801 tatgaggatg aagaaccgat gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct
     7861 cgtggcaaac gcggctggct atggctgctg ttaaaactgg ctatcgtttt tgccgtgctg
     7921 atcgccattt acggcgttta tctcgatcaa aaaattcgta gccgtattga tggcaaggtc
     7981 tggcaactgc ccgcggcagt ttatggccga atggtcaacc ttgaaccaga tatgaccatc
     8041 agcaagaacg aaatggtgaa gctgctggag gcaacacagt atcgtcaggt gtcgaaaatg
     8101 acccgtcctg gcgaatttac cgtgcaggcc aacagcattg agatgatccg ccgtccgttt
     8161 gatttcccgg acagcaaaga agggcaggtg cgtgcgcgtc tgacctttga tggcgatcat
     8221 ctggcgacga tcgtcaatat ggagaacaac cgtcagttcg gtttcttccg tcttgatccg
     8281 cgcctgatca ccatgatctc ttcgccaaac ggtgagcagc gtctgtttgt gccgcgcagt
     8341 ggtttcccgg acttgctggt ggatactttg ctggcgacag aagaccgtca tttctacgag
     8401 catgatggga tcagtctcta ctccatcgga cgtgcggtgc tggcaaacct gaccgccgga
     8461 cgcacggtgc agggagcgag tacgctgacg caacagctgg tgaaaaacct gttcctctcc
     8521 agcgagcgtt cttactggcg taaagcgaac gaagcttaca tggcgctgat catggacgcg
     8581 cgttacagca aagaccgtat tcttgagctg tatatgaacg aggtgtatct cggtcagagc
     8641 ggcgacaacg aaatccgcgg cttcccgctt gcgagcttgt attactttgg tcgcccggta
     8701 gaagagctca gcctcgacca gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc
     8761 tacaacccgt ggcgtaaccc gaaactggcg ctggagcgac gtaatctggt gctgcgtttg
     8821 ctacaacaac agcagattat cgatggtgaa ctgtatgaaa tgctcagcgc gcgtcctctg
     8881 ggcgtacagc cgcgcggtgg ggtaatctct ccacagccag cctttatgca actggtgcgt
     8941 caggagctgc aggcgaaatt aggcgataag gtaaaagatc tctccggcgt gaagatcttc
     9001 actacctttg actcggtggc ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg
     9061 gcactgaaga aacagcgtaa gttgagcgat cttgaaactg caattgtggt cgtcgaccgc
     9121 tttagtggtg aagttcgtgc gatggtggga ggttctgagc cgcagtttgc gggctacaac
     9181 cgtgcgatgc aggcgcgtcg ttcgattggt tcccttgcaa aaccagcgac ttatctgacg
     9241 gctttaagcc agccgaaaat ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg
     9301 ctgcgtcagc cgaatggcca ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa
     9361 agcggcagag tgatgctggt ggatgcgttg acccgttcga tgaacgtgcc gacggtaaat
     9421 ctggggatgg cgctggggct gcctgcagtt acggagacct ggatcaaact gggcgtgccg
     9481 aaagatcagc tgcatccggt tccggcgatg ctgctggggg cgttgaactt aacgccaatc
     9541 gaagtggcgc aggcattcca gaccatcgcc agcggcggta accgtgcacc gctttctgca
     9601 ctgcgttcgg taatcgcgga agatggcaag gtgctgtatc agagcttccc gcaggcagaa
     9661 cgcgctgttc cggcgcaggc ggcgtatctg acactatgga ccatgcagca ggtggtacaa
     9721 cgtggtacgg gtcgtcaact tggggcgaaa tacccgaacc tgcatctggc agggaaaaca
     9781 gggactacca acaataacgt agatacctgg tttgcgggca ttgacggcag cacggtgacc
     9841 atcacctggg taggccgtga taacaaccag ccgaccaaac tgtatggtgc cagcggggca
     9901 atgtctattt atcagcgtta tctggctaac cagacgccaa cgccgctgaa tcttgttccg
     9961 ccagaagata ttgcagatat gggcgtggac tacgacggca actttgtttg cagcggtggc
    10021 atgcgtgtct tgccggtctg gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg
    10081 cagcagcagc cgtcaggcaa tccgtttgat cagtcttctc agccgcagca acagccgcaa
    10141 cagcaacctg ctcagcaaga gcagaaagac agcgacggtg tagccggttg gatcaaggat
    10201 atgtttggta gtaattaaca tctaagcgtg aaataccgga tggcgaattg ccatccggta
    10261 aaataacatc ccacctaaag tattaaccct ttcttttcat ctggttgttt attaaccctt
    10321 caggaacgct cagattgcgt accgcttgcg aacccgccag cgtttcgaat attatcttat
    10381 ctttataata atcattctcg tttacgttat cattcacttt acatcagaga tataccaatg
    10441 gcgcgttcca aaactgctca gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc
    10501 acagcggtta gcggcatgtc tgtttatgca caggcagcgg ttgaaccgaa agaagacact
    10561 atcaccgtta ccgctgcacc tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt
    10621 gcggcgaagc actctgctac tgcgactaaa acggatacac caattgaaaa aacgccgcag
    10681 tctatttcgg ttgtgacaaa tgaagagatg cagatgcgtc aatttcagtc tgtaaaagaa
    10741 gcattaggtt atacacctgg cgttacggtt agcagtcgtg gtgcttctaa tacatatgat
    10801 tttgttatca ttcgtggttt ctcatctgtt ggtctgaatc aaaataatta ccttgatgga
    10861 ctaaaacttc agggcaactt ttataacgat gctgtgattg atccttacat gcttgagagg
    10921 gttgaactga tgcgtgggcc gacgtctgtt ctctatggaa aaagtaatcc tggtgggatt
    10981 atttctatgg tgagtaagcg tccgaccact gaacccctga aagaaattca gtttaaaatg
    11041 gggacggata atctgtttca gaccggattc gatttcagtg atgcactgga tgataacggt
    11101 gagttctctt accgtttgac gggcctcgct cgctcaacaa acgaacagca gaaaaactct
    11161 gaatctcagc gttataccat tgctccatca ttctcatggc gtccagacga caaaaccaat
    11221 tttactttcc tgtcctattt ccagaatgaa ccagaaacgg gttattacgg ttggttgccg
    11281 aaagagggga ccgttgagcc attgcctaat ggtaagcgtc taccgactga cttcaatgaa
    11341 ggtgcatcga ataatacata ctcccgtaac cagaaaatgg tgggatatag ttttgaacat
    11401 ggttttaatg acaccttcac cgtgcgtcag aatctgcgtt tcagtgaaat gaaaacctca
    11461 cagaaaagtg tttatggcac agggattgct ggtgatggtc ataccctaaa ccgcgggaca
    11521 gtggtggata atgagcgtct acaaaacttt agcgttgata cccaacttga aagtaagttt
    11581 gctacaggtg acgtcgagca tactttgctg acaggggtag acttcatgcg gatgcgtaat
    11641 gatatcaatg ccagctttgg atccgcacca tccatcgatc tttataacaa atatcaccct
    11701 gaatactttg catttggtag cgcagagcca taccagatga atgaaagcaa acaaacaggt
    11761 atttatgttc aggatcaggc ggaatggaat aaatgggtat tcactctggg gggacgctac
    11821 gattggtcta agcaagcgac cactgttcgt gaaaactctt atacgccgac tgaaggttat
    11881 attgagcgca atgatcatca gttcacctgg cgcggtggtg taaattactt attcgataat
    11941 ggtatttcac cttactttag ctatagccag tcctttgaac cgagtgcttt cgatctgtgg
    12001 agcaacccgc gcatttccta taagccatcg aaaggtgaac agtatgaagc tggcgtaaaa
    12061 tatgttccga atgatatgcc ggtcgttgtt acgggcgcag tctatcaatt gacgaaaaca
    12121 aataacctga cagcagaccc aacaaaccca ttagcgcaag tcccagcagg tgagattcgc
    12181 gctcgtggtg tggaacttga agcaaaagcg gcgttaaatg ccaatattaa cttgacggct
    12241 tcttatacct acaccgatgc ggaatacacc aaagacacca atctcaaagg taaaactcca
    12301 gaacaagtac cggagcatat ggcatctctc tggggggatt ataccttcaa tgaagggccg
    12361 ctttctggtt taacattggg aacaggtggt cgttttattg gttccagcta tggtgatccg
    12421 gcaaacactt ttaaagtggg tagcgcagct gtaatggatg ctgttgtaaa atatgatctg
    12481 gcacgctttg gtatggcggg atccagcctt gctgtgaacg tcaacaattt gctcgatcgt
    12541 gagtatgttg ccagttgctt ccagacttat ggctgcttct ggggcgcaga acgtcaggtc
    12601 gttgcaaccg caaccttccg tttctaattt ctcttttggg gcacggattt ccgtgcccat
    12661 ttcacaagtt ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc
    12721 gtaatatctc ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc
    12781 ctgccgggaa agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca
    12841 aaatgcttgg tcgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc
    12901 tggaaagctg gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc
    12961 ctccggcaga agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg
    13021 gcgcgctggg gcgctttggt gcggcagatc gcgaaaaggt cgaggaagct atctcgctgg
    13081 ttggcttaaa accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcagc
    13141 gggcgtggat cgccatgctg gtggcgcagg atagccgttg tctgttgctc gacgaaccga
    13201 cttcggcgct ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc
    13261 aggaacgtgg cctgacggtc attgccgtgt tgcacgatat caatatggcg gcacgctact
    13321 gtgattatct ggtcgccctg cgcagtggtg aaatgattgc tcagggaacg cctgcggaaa
    13381 ttatgcgcgg cgaaaccctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc
    13441 cggcgggtgc tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg
    13501 ccgtcgactg ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca
    13561 cgcggcggct attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact
    13621 gctggcgctc ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt
    13681 cagcgaacca ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct
    13741 tgaactgctg accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc
    13801 atcagaaatg ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca
    13861 gccgttggcg atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag
    13921 cgcagcggaa acgcatttag cgcaatacga agactttatc cgcagcatga aaccccgctt
    13981 tgtgaagcgt ggcgcacgcc cgttactgct gacgacgctt atcgatccgc gccatatgct
    14041 ggtcttcggt ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc
    14101 ctggcaaggg gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcagc
    14161 gtataaagac gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct
    14221 aatggcaacc ccgctgtggc aggccatgcc gtttgtccgc gccggacgct ttcagcgcgt
    14281 acctgcagtc tggttttatg gtgcgacact ctcggcaatg cactttgtgc gcgttctgga
    14341 taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc tttttccggc gttattgctg
    14401 gcgctgttag tgattgtcgc tacggcgctc acctggatga acttctcgca ggcgctgccg
    14461 cgtagccagt gggcgcaggc tgcctggtcg ccagacatcg atgtcatcga gcagatgatt
    14521 tttcactaca gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgt gctgtttcag
    14581 caagtgctgc gtaacccgct ggcggagccg acgacgcttg gcgttgctac aggcgcgcaa
    14641 ctgggaatta ccgtcaccac actttgggcg atcccaggtg ctatggcgag ccagtttgcc
    14701 gcactggtgg gcgcttgtgt cgttggctta atcgtctttg gcgtcgcgtg ggggaaacgg
    14761 ctttcgccgg ttacgcttat tctcgcgggg ctggtggtga gcctttattg cggggcaatt
    14821 aatcagctac tggttatctt ccatcacgac cagctgcaaa gcatgttcct gtggagcacc
    14881 ggaacgctga cgcaaaccga ctggggcggc gttgagcgtt tatggccgca gttgctgggc
    14941 ggcgtgatgt tgacgttatt gctacttcgc ccgttaaccc tgatggggct tgatgatggc
    15001 gtggcgcgca atctcgggct ggccttgtcg ctcgcgcgtc tggcggcgct gtcgctggcg
    15061 atagtcatca gtgcgctgct ggtgaatgcg gtggggatta tcggctttat cggtttgttc
    15121 gcgccgctgc tggcgaaaat gctgggggcg cggcgtttgt tgccacggtt gatgctggcg
    15181 tcgctgattg gggcgctgat cctctggctt tccgatcaaa tcatcctctg gctgactcgc
    15241 gtgtggatgg aagtgtccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg
    15301 tggctgttgc cgcgtttacg cagcattagc gcgccggata tgaaggtcaa cgatcgtgtc
    15361 gcggctgaac gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct gctgttgatg
    15421 gctgtggtgg tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg
    15481 gcgttgctcg aggatttgat gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg
    15541 ggcgtcatgc tggcggtggc gggctgtatt attcagcggc tgaccggaaa cccgatggca
    15601 agcccggaag tgctggggat tagctctggc gcggcgtttg gcgtggtgtt aatgctgttt
    15661 ctggtgccgg gtaatgcctt tggctggctg ttacccgcgg gcagtatcgg ggcggcggtg
    15721 acgctgttga tcattatgat cgccgccggc cgcggtggat tttccccaca ccgtatgtta
    15781 ctggcgggga tggcgttaag taccgcattc accatgcttt tgatgatgtt gcaggcgagc
    15841 ggtgacccgc gaatggcgca agtgctgacc tggatttccg gttcgaccta caacgcgacc
    15901 gatgcgcagg tctggcgtac cggaattgtg atggtgattt tgctggcgat taccccgctg
    15961 taccgccgct ggctgaccat tttaccgctg ggtggtgata ccgcacgagc cgtaggaatg
    16021 gcgctgacgc cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac ggcgaccgcg
    16081 acgatgacta ttggaccgtt gagttttgtt ggtttaatgg caccgcatat tgcgcggatg
    16141 atgggctttc gacggacgat gccacacatc gtaatttcgg cgctggtggg tggtttactg
    16201 ctggtattcg ctgactggtg tgggcggatg gtgctgtttc cattccagat cccggcgggg
    16261 ctgctgtcga cctttatcgg cgcgccatat tttatctatt tgctgagaaa gcagagccgt
    16321 taatttttcc atataacggc cctgtacgcc tggacggata aagcgtttac gcgacaacgg
    16381 atgcccgatg cgacgctggc gcgtcttatc gggcctacaa acggccccga atcgtaggtc
    16441 ggataaggcg ttcacgccgc atccgacaaa ccatgctgga tcacaacttc gcaaacaccc
    16501 gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccacg gacataaagc
    16561 ccgcttcaaa cgctgacggt gccaggtaaa caccttcgtc cagcatcata tggaagaaac
    16621 gcttaaagcg ttccacgtca caggccatca catcctgata gcacgtcacg gactcggcgt
    16681 cggtaaagaa aataccgaac atgccgccaa cgtggttaac gaccagcgga attccggctt
    16741 cttctgccgc ttccagcaga ccttctgcca gacgtgttgt cagctcatcc aacgtttcgt
    16801 gaacgcccgg ttgcgcgact tcattcagac aggcgaaacc ggctgccatc gcaattgggt
    16861 taccggaaag cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca tccattacat
    16921 cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttg cccaggcagg
    16981 tgagatccgg ttccacaccg taataatcct gtgcgccagc cagtgccacg cggaagccgg
    17041 tcattacttc atcgatgatc agcaatgcgc caaattcgtc gcacagcgca cgcagacctg
    17101 gcaggaagtc tggcagcggt ggaacacagt tcatattgcc tgccaccggc tcgatgataa
    17161 tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaagcc agatcgttat
    17221 aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc
    17281 ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac
    17341 acccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccaga cggatggcgc
    17401 tcatcgtcgc ctcggtgccg gagttcacca tgcgcaccat atccatggtc gggaccaatt
    17461 cggtcaccag ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag cttaaaccac
    17521 gctcggcggc ttcaatcacg gcattgcgga ttgccggatg gttatggccc agcaccatcg
    17581 gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc
    17641 cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg
    17701 agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca
    17761 tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa
    17821 gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta
    17881 taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag
    17941 taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact
    18001 ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt
    18061 cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt
    18121 gttgggctgg cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg
    18181 ggggcgctgg tacatactgc tgataattat ccgcttctgt taaccgtcgc ttttctctgt
    18241 tcggcggtgc tggcgatgtt tggctacttt ttggtgcgca aatacgcgcc ggaagcaggt
    18301 ggttcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcccgt tcgctggtgg
    18361 cgtgtattgc cggtgaagtt ctttggcggg ctggggacac tcggcggagg catggtgttg
    18421 gggcgcgaag ggccaaccgt gcagatcggc ggtaacattg gccgtatggt gcttgatatt
    18481 ttccgcctga aaggtgacga agctcgccat acgctgctgg caaccggtgc tgctgcgggg
    18541 ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt
    18601 ccgcagtttc gctatacgtt aatttcgata aaagcggtat ttattggcgt cattatgtcg
    18661 accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct
    18721 gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc
    18781 ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc
    18841 ggcaatatta ccaaatgggt actgatgggc ggtgcgattg gcggtctgtg tggattgctg
    18901 gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg
    18961 gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta
    19021 ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact
    19081 gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcaccttgag
    19141 gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg
    19201 ttaacgggga tcattctggt tctggagatg accgataact accagctcat tttgccaatg
    19261 attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa accgctatac
    19321 tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag
    19381 gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta
    19441 ttattgggtt agaatttgcc caattgccga tgtcgtttgg agcaaaatat gagtgatgac
    19501 gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct
    19561 gacgaagata acccgaatct gaaattacgc gtgtatatca caggtggcgg ttgcagcggc
    19621 ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa
    19681 cagggcgttg gcttggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt
    19741 gactataccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc
    19801 acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta
    19861 tccggcgcat cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa
    19921 gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg
    19981 ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa
    20041 tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatctcgcgg gccagtacat
    20101 cacgaatgat cccgccgcca acgccagtaa tgacgaccat acaaaccgcg attaacggac
    20161 cgacttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg
    20221 cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg
    20281 tcagcatgct ggttaccatt gcaacgacca gatcggtggg atctttcacc caaaataccg
    20341 ggccgtgatc cagcgccatg tcgcgaattg tcccgccgcc tacagcggta accacgccca
    20401 gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg
    20461 caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga
    20521 gcacctaaaa aggtcaggcg ctaatctacc tgtgaaagcg cattacagag ctgttgtgca
    20581 gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga
    20641 attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta
    20701 atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga
    20761 acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg gttttgaatc
    20821 gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta
    20881 ggtttatcag catattgcgc tttcaattgc gcgtactgat ccagcaggga ttgcgcggct
    20941 tgttcggcct tatccggttg cggactccag ggggccaatt gacgtaacgc attggcaatt
    21001 tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca
    21061 acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca
    21121 atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga
    21181 ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca
    21241 gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcaaga
    21301 aaagacaggg cgaccagcgc cctgaacagt gacttagcca tgtgccagtt tctgcaccag
    21361 tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag
    21421 atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa
    21481 attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa
    21541 gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat
    21601 caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag
    21661 tttatcgtca gctttaaagc ctgccggaca gcctggtaac tgaccgtatt cataaccaaa
    21721 tgccgtgaca tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt
    21781 cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg
    21841 ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag
    21901 cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga gactgatagt
    21961 ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat
    22021 gccgattttc atagatttac tcgcgataag cccgatttga aagcatagtt taccatgcgc
    22081 ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa
    22141 aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc
    22201 tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc
    22261 aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc
    22321 actcgatgga agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga
    22381 aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca
    22441 ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg
    22501 aagcagcgat aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc
    22561 ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaacagc
    22621 ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag
    22681 gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta
    22741 ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt
    22801 taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag
    22861 aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg
    22921 acatctccta ttgtgtggca gacctt
//
LOCUS       DAZOZY010000049        22433 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZOZY010000049 DAZOZY010000000
VERSION     DAZOZY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22433
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.049"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..223)
                     /locus_tag="ACHURQ_001657"
     CDS             complement(<1..223)
                     /locus_tag="ACHURQ_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0823463.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
     gene            complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHURQ_001658"
     CDS             complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHURQ_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR0823464.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHURQ_001659"
     CDS             complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHURQ_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR0823465.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1466..1594
                     /locus_tag="ACHURQ_001660"
                     /pseudo
     CDS             1466..1594
                     /locus_tag="ACHURQ_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHURQ_001661"
     CDS             complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHURQ_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR0823466.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2149..3807
                     /gene="fliF"
                     /locus_tag="ACHURQ_001662"
     CDS             2149..3807
                     /gene="fliF"
                     /locus_tag="ACHURQ_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR0823467.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3800..4795
                     /gene="fliG"
                     /locus_tag="ACHURQ_001663"
     CDS             3800..4795
                     /gene="fliG"
                     /locus_tag="ACHURQ_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR0823468.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4788..5474
                     /gene="fliH"
                     /locus_tag="ACHURQ_001664"
     CDS             4788..5474
                     /gene="fliH"
                     /locus_tag="ACHURQ_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR0823469.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5474..6847
                     /gene="fliI"
                     /locus_tag="ACHURQ_001665"
     CDS             5474..6847
                     /gene="fliI"
                     /locus_tag="ACHURQ_001665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR0823470.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHURQ_001666"
     CDS             6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHURQ_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR0823471.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7306..8433
                     /locus_tag="ACHURQ_001667"
     CDS             7306..8433
                     /locus_tag="ACHURQ_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR0823472.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8538..9002
                     /gene="fliL"
                     /locus_tag="ACHURQ_001668"
     CDS             8538..9002
                     /gene="fliL"
                     /locus_tag="ACHURQ_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR0823473.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9007..10011
                     /gene="fliM"
                     /locus_tag="ACHURQ_001669"
     CDS             9007..10011
                     /gene="fliM"
                     /locus_tag="ACHURQ_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR0823474.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10008..10421
                     /gene="fliN"
                     /locus_tag="ACHURQ_001670"
     CDS             10008..10421
                     /gene="fliN"
                     /locus_tag="ACHURQ_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR0823475.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10424..10789
                     /gene="fliO"
                     /locus_tag="ACHURQ_001671"
     CDS             10424..10789
                     /gene="fliO"
                     /locus_tag="ACHURQ_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR0823476.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10789..11526
                     /gene="fliP"
                     /locus_tag="ACHURQ_001672"
     CDS             10789..11526
                     /gene="fliP"
                     /locus_tag="ACHURQ_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR0823477.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHURQ_001673"
     CDS             11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHURQ_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR0823478.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11814..12599
                     /gene="fliR"
                     /locus_tag="ACHURQ_001674"
     CDS             11814..12599
                     /gene="fliR"
                     /locus_tag="ACHURQ_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR0823479.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHURQ_001675"
     CDS             12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHURQ_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR0823480.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHURQ_001676"
     CDS             complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHURQ_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR0823481.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13902..14129
                     /gene="yodD"
                     /locus_tag="ACHURQ_001677"
     CDS             13902..14129
                     /gene="yodD"
                     /locus_tag="ACHURQ_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR0823482.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14427..15242
                     /locus_tag="ACHURQ_001678"
     CDS             14427..15242
                     /locus_tag="ACHURQ_001678"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR0823483.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHURQ_001679"
     CDS             complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHURQ_001679"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR0823484.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17104..17286)
                     /locus_tag="ACHURQ_001680"
     CDS             complement(17104..17286)
                     /locus_tag="ACHURQ_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR0823485.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17365..18282)
                     /locus_tag="ACHURQ_001681"
     CDS             complement(17365..18282)
                     /locus_tag="ACHURQ_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR0823486.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18455..19376
                     /gene="yedA"
                     /locus_tag="ACHURQ_001682"
                     /pseudo
     CDS             18455..19376
                     /gene="yedA"
                     /locus_tag="ACHURQ_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHURQ_001683"
     CDS             complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHURQ_001683"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR0823487.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHURQ_001684"
     CDS             complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHURQ_001684"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR0823488.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21301..21957)
                     /locus_tag="ACHURQ_001685"
     CDS             complement(21301..21957)
                     /locus_tag="ACHURQ_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR0823489.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHURQ_001686"
     CDS             complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHURQ_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR0823490.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5501 a         5900 c         5685 g         5347 t
ORIGIN      
        1 gacctgcttc tgggtaattc gcaatataag attcatatcc acagggaaat aatattatct
       61 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
      121 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
      181 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
      241 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
      301 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
      361 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
      421 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
      481 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
      541 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
      601 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
      661 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
      721 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
      781 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
      841 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
      901 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
      961 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     1021 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     1081 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     1141 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     1201 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     1261 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     1321 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     1381 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     1441 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     1501 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     1561 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     1621 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     1681 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     1741 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     1801 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     1861 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     1921 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     1981 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     2041 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     2101 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     2161 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     2221 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     2281 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     2341 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     2401 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     2461 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     2521 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     2581 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     2641 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     2701 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     2761 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     2821 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     2881 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     2941 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3001 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     3061 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     3121 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     3181 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     3241 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     3301 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     3361 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     3421 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     3481 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     3541 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     3601 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     3661 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     3721 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     3781 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     3841 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     3901 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     3961 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4021 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     4081 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     4141 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     4201 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     4261 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     4321 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     4381 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     4441 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     4501 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     4561 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     4621 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     4681 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     4741 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     4801 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     4861 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     4921 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     4981 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     5041 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     5101 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     5161 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     5221 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     5281 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     5341 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     5401 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     5461 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     5521 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     5581 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     5641 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     5701 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     5761 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     5821 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     5881 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     5941 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     6001 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     6061 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     6121 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     6181 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     6241 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     6301 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     6361 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     6421 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     6481 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     6541 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     6601 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     6661 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     6721 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     6781 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     6841 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     6901 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     6961 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7021 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     7081 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     7141 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     7201 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     7261 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     7321 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     7381 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     7441 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     7501 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     7561 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     7621 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     7681 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     7741 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     7801 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     7861 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     7921 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     7981 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     8041 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     8101 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     8161 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     8221 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     8281 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     8341 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     8401 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     8461 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     8521 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     8581 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     8641 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     8701 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     8761 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     8821 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     8881 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     8941 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
     9001 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     9061 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     9121 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     9181 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     9241 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     9301 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
     9361 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
     9421 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
     9481 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
     9541 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     9601 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
     9661 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     9721 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     9781 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     9841 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
     9901 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     9961 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    10021 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    10081 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    10141 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    10201 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    10261 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    10321 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    10381 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    10441 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    10501 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    10561 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    10621 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    10681 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    10741 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    10801 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    10861 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    10921 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    10981 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    11041 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    11101 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    11161 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    11221 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    11281 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    11341 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    11401 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    11461 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    11521 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    11581 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    11641 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    11701 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    11761 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    11821 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    11881 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    11941 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    12001 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    12061 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    12121 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    12181 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    12241 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    12301 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    12361 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    12421 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    12481 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    12541 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    12601 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    12661 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    12721 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    12781 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    12841 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    12901 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    12961 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    13021 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    13081 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    13141 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga taagttctaa
    13201 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    13261 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    13321 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    13381 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    13441 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    13501 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    13561 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    13621 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    13681 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    13741 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    13801 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    13861 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    13921 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    13981 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    14041 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    14101 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    14161 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    14221 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    14281 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    14341 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    14401 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    14461 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    14521 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    14581 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    14641 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    14701 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    14761 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    14821 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    14881 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    14941 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    15001 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    15061 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    15121 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    15181 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    15241 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    15301 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    15361 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    15421 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    15481 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    15541 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    15601 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    15661 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    15721 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    15781 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    15841 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    15901 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    15961 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    16021 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    16081 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    16141 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    16201 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    16261 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    16321 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    16381 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    16441 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    16501 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    16561 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    16621 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    16681 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    16741 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    16801 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    16861 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    16921 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    16981 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    17041 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    17101 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    17161 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    17221 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    17281 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    17341 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    17401 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    17461 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    17521 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    17581 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    17641 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    17701 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    17761 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    17821 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    17881 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    17941 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    18001 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    18061 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    18121 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    18181 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    18241 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    18301 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    18361 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    18421 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    18481 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    18541 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    18601 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    18661 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    18721 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    18781 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    18841 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    18901 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    18961 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    19021 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    19081 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    19141 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    19201 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    19261 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    19321 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    19381 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    19441 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    19501 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    19561 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    19621 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    19681 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    19741 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    19801 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    19861 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    19921 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    19981 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    20041 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    20101 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    20161 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    20221 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    20281 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    20341 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    20401 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    20461 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    20521 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    20581 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    20641 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    20701 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    20761 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    20821 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    20881 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    20941 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    21001 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    21061 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    21121 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    21181 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    21241 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    21301 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    21361 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    21421 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    21481 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    21541 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    21601 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    21661 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    21721 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    21781 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    21841 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    21901 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    21961 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    22021 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    22081 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    22141 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    22201 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    22261 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    22321 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    22381 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa tta
//
LOCUS       DAZOZY010000050        21984 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZOZY010000050 DAZOZY010000000
VERSION     DAZOZY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21984
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.050"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHURQ_001687"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHURQ_001687"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR0823491.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHURQ_001688"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHURQ_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR0823492.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHURQ_001689"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHURQ_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR0823493.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHURQ_001690"
     CDS             2284..2730
                     /locus_tag="ACHURQ_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR0823494.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHURQ_001691"
     CDS             complement(2687..3508)
                     /gene="nimR"
                     /locus_tag="ACHURQ_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFR0823495.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3605..4786
                     /gene="nimT"
                     /locus_tag="ACHURQ_001692"
     CDS             3605..4786
                     /gene="nimT"
                     /locus_tag="ACHURQ_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR0823496.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4841..5188
                     /locus_tag="ACHURQ_001693"
     CDS             4841..5188
                     /locus_tag="ACHURQ_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR0823497.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHURQ_001694"
     CDS             complement(5210..5464)
                     /gene="yoaF"
                     /locus_tag="ACHURQ_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR0823498.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHURQ_001695"
     CDS             5647..6672
                     /gene="dgcP"
                     /locus_tag="ACHURQ_001695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR0823499.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6705..6803
                     /locus_tag="ACHURQ_001696"
     CDS             6705..6803
                     /locus_tag="ACHURQ_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR0823500.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHURQ_001697"
     CDS             6806..6880
                     /gene="yoaJ"
                     /locus_tag="ACHURQ_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR0823501.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6939..7187)
                     /locus_tag="ACHURQ_001698"
     CDS             complement(6939..7187)
                     /locus_tag="ACHURQ_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0823502.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7335..7517)
                     /locus_tag="ACHURQ_001699"
     CDS             complement(7335..7517)
                     /locus_tag="ACHURQ_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR0823503.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHURQ_001700"
     CDS             complement(7521..7880)
                     /gene="yeaR"
                     /locus_tag="ACHURQ_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR0823504.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHURQ_001701"
     CDS             complement(8053..8691)
                     /gene="leuE"
                     /locus_tag="ACHURQ_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR0823505.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHURQ_001702"
     CDS             complement(8818..9741)
                     /gene="dmlR"
                     /locus_tag="ACHURQ_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR0823506.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHURQ_001703"
     CDS             9844..10929
                     /gene="dmlA"
                     /locus_tag="ACHURQ_001703"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR0823507.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHURQ_001704"
     CDS             11180..12790
                     /gene="yeaV"
                     /locus_tag="ACHURQ_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR0823508.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHURQ_001705"
     CDS             12822..13946
                     /gene="yeaW"
                     /locus_tag="ACHURQ_001705"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR0823509.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHURQ_001706"
     CDS             14002..14967
                     /gene="yeaX"
                     /locus_tag="ACHURQ_001706"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR0823510.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHURQ_001707"
     CDS             complement(15021..16136)
                     /gene="rnd"
                     /locus_tag="ACHURQ_001707"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR0823511.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHURQ_001708"
     CDS             complement(16218..17903)
                     /gene="fadD"
                     /locus_tag="ACHURQ_001708"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR0823512.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHURQ_001709"
     CDS             complement(18108..18689)
                     /gene="yeaY"
                     /locus_tag="ACHURQ_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR0823513.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHURQ_001710"
     CDS             complement(18729..19424)
                     /gene="tsaB"
                     /locus_tag="ACHURQ_001710"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR0823514.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19482..21394)
                     /locus_tag="ACHURQ_001711"
                     /pseudo
     CDS             complement(19482..21394)
                     /locus_tag="ACHURQ_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
     gene            21526..21870
                     /locus_tag="ACHURQ_001712"
     CDS             21526..21870
                     /locus_tag="ACHURQ_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR0823515.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5374 a         5646 c         5366 g         5598 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt tactatcagt cgcgcaagat
     2941 tgcgttcact catcgcaaaa aagccagccc attgccctaa tgacccccac tcgacgggcc
     3001 ctttcgccat catctccacc atcgtgcgga ttttaggatg agaagaaacg ggcaggtgca
     3061 gctgttgttg tggctgctga gggagttcat caaacaggac ctgaattaat ctgcgggtca
     3121 tcggctcagc acgctgagtc gtcgtcctgt ttgccagggt taaaatcaat tcccggcata
     3181 agggcgatat tttcaatgtg caacatgttg tcggcattgt cactgcgctg ggttcgataa
     3241 acagaaaaca gagttcagcg ttagctgtca cttgattgct atgttccacc ccgcccggta
     3301 tccaaaccgc atattgcggc ggcaccatcc acaaagcatt ttccaccgta caggtaattg
     3361 caccatgtaa tgctagtatc agctgtcctt tgcggtgctg atgtacagga ctggttaact
     3421 catccgttcc ggcgtgaata caaaatgcga ccgcggcttc atggtgtcgg tcgggttcat
     3481 agccattgag attcaacctg tgcatcattt tgtccgaatt tagcgataat ttgtcatttt
     3541 agcttgattc aacataacaa taaaaacggt aaggtacagc ctcgtttgta acaatgagaa
     3601 gcatatgacc tgttcaactt cattaagcgg caaaaacagg attgtcctta tcgctggcat
     3661 tctgatgatt gccacaacat tacgcgtcac ctttatcggc gcagcaccgt tactggatac
     3721 gattcgttcc gcttactcgc tgacgacagc gcaaaccggc ttattgacca ccctgccatt
     3781 attggccttt gcgctaatct cacctttggc caccccggta gcgcgacgtt ttggtatgga
     3841 acgtagcctg tttgccgcgt tacttttgat ctgtgctggt atcgcaattc gctctctccc
     3901 ttcgccttac ttattatttg gcggtacagc ggtcattggc ggtgggattg cattaggcaa
     3961 tgtcttactg ccaggattaa ttaaacgcga tttccctcat tccgtcgcca gacttaccgg
     4021 cgcatattcc ctgacaatgg gagctgcagc ggcactggga tcggctatga tagtgccgct
     4081 ggctttgaac ggttttggct ggcaaggcgc gttgctcatg ctgatgtgtt ttcctctgct
     4141 ggctcttttt ttatggctgc cacagtggcg aagccaacaa catgcaaatt tgagtacctc
     4201 gcgcgcctta catactcggg gtatctggcg ttcgccgctt gcctggcagg taacattgtt
     4261 tcttgggatc aactcactga tctattacgt gattattggc tggcttccgg cgatcctcat
     4321 cagtcacggc tatagcgaag cacaggcggg ttcactgcat ggtttgctgc aactagccac
     4381 agcagctccc ggtttgctga tccctctttt cttacatcat gtgaaagatc agcgtggtat
     4441 tgcagcgttc gttgccttga tgtgcgcagt gggcgctgtt gggctctgct ttatgccagc
     4501 gcacgcgatc acctggactc tgcttttcgg ttttggttcc ggcgcaacaa tgatactggg
     4561 gttgacgttc attggtcttc gggcaagttc tgcgcatcag gcggcggcac tctcggggat
     4621 ggcacaatcc gtcgggtatt tgctggcagc ctgtgggccg ccgctaatgg gtaaaataca
     4681 cgatgctaac ggtgactggt ccgtaccact gatgggtgtt gcaatacttt cactactgat
     4741 ggcgattttc ggactttgcg ccgggagaga caaagaaatt cgctaatatc cggtgctata
     4801 gtgacgtgac aaatcatgcg tgaaagggag aacaaacacg atgaatattc agtgcaaacg
     4861 cgtttatgat ccggctgaac agagcgatgg ttatcgcgta ctggtcgacc gcctctggcc
     4921 gcgcggtatc aaaaaaaccg atttagccct tgatgagtgg gataaagaaa tcacgccgtc
     4981 aacggaactg cgcaaagcct ttcacggcga agtcgtcgat tttgcaacct ttcgcgagca
     5041 atatcttgca gaactggcgc aacacgagca agaaggaaag cggctggcgg acatcgccaa
     5101 aaaacagccg ctgaccctgc tctacgcagc aaaaaacacc acgcagaacc atgcgctggt
     5161 gctggccgac tggctacgta gcttgtgatt ttagtacagc atccggcggt tatttttcac
     5221 cagccggatg gtcccgtcgc cacaatgccc attcatcaat cgtttcaccg cccggtaatt
     5281 tgcaattgtt gctgacccct tgcgctgtcg acactggaat gagcgtcccg cccttctgct
     5341 ggcaatagac cgacgccgga tttgccatac caatctgcgg cggtttaggt acttctggct
     5401 gagaagaggt tgaacaacca gccaggacca gcaagcaagg caaagcaaaa ctgataattt
     5461 tcatttattg atctcactca tttatctacg cttagagtat cgcggtatcg ttttgttttg
     5521 cagcactatt tttattacat tcactcaaaa catattacgt cttgtttcat ctttgttggt
     5581 gatgttttat catgcctgca aagattaaat aatcagcatt tacccgccgt atcctggagt
     5641 tgtttcgtgt cagatcagat tatcgcccgc gtctcgcaat cccttgccaa agaacagtca
     5701 ctggaaagtc tggtccgaca gcttctggag atgctggaaa tggtcactga tatggaatca
     5761 acctacctga ccaaagtgga tgtcgaagcg cgcctgcagc atattatgtt tgcccgtaac
     5821 agccagaaaa tgcacatccc ggagaatttt accgtctcgt gggattactc gttatgcaaa
     5881 cgcgccattg atgaaaactg ctttttcagc gatgaagtcc ctgaccgttg gggcgactgt
     5941 attgcggcac gcaatcttgg catctccaca tttctgagca caccaattca cttaccggat
     6001 ggatcattct atggcacgct ttgcgccgcc agcagtgaga agcgccagtg gagtgaacgc
     6061 gcggaacagg ttttacagtt attcgccgga ctgattgcac aatatattca aaaagaggca
     6121 ctggttgaac agttgcgcga agccaatgct gcgctgattg cgcaatcgta taccgactcg
     6181 ttaaccgggt tgccgaatcc gcgggcgatt tttgaaaatc tgacgacgct gttttctctc
     6241 gcccggcatc ttaaccataa gataatgatc gcgtttatcg atctggataa cttcaaatta
     6301 atcaatgatc gttttggtca taatagtggc gatctgtttc tcattcaggt tggcgagcgc
     6361 cttaatacgc tccagcaaaa tggcgaagtt attggtcgtc tcggcggtga tgagtttttg
     6421 gttgtttcac taaacaacga gaatgcggat atttcgtcgc tgcgagaacg cattcagcag
     6481 caaatacgtg gagaatatca cttaggtgat gttgatttgt attatcccgg tgccagtctt
     6541 ggcatagtag aagtcgatcc tgaaacaacc gatgcagaca gtgccctgca tgctgccgat
     6601 attgcgatgt atcaggagaa aaaacacaaa cagaaaacac cttttgtcgc gcattcagcg
     6661 ccacattgct gaggcgtatt cacatccttt tgattggtga taacatgcga atcggtatta
     6721 tttttccggt tgtaatcttt attacagcgg tcgtattttt agcatggttt tttattggcg
     6781 gctatgctgc cccgggggca taaagatgaa aaaaacaacg attattatga tgggtgtggc
     6841 gattattgtc gtactcggca ctgagctggg atggtggtaa cgtcacctct aaaaaatagc
     6901 aaaggctgcc tgtgtgcagc ctttgtgcaa tttaagcgtt aacttttaat cttcctgtag
     6961 ataaatagca cgacaatcgc accaataacg gcaaccacga agctgccaaa attgaagcca
     7021 tcgactttac caaagccaaa cagcgtgctg atccatccgc cgactacggc accgactatc
     7081 cccagcagga tagtcataaa gaatccacca ccatctttac ctggcatgat ccacttcgcc
     7141 agaataccgg caataagccc aaaaataatc catgacagaa tgcccattgt ttcctcactt
     7201 atctgttttg cattagcggg ttagtcgctg ataaaaagca tagcacaaca tcgggagggc
     7261 aagatttgtg acgagcatca cggaggattt ttttgcgatg gcgcagaaat tgcgccatca
     7321 acgatcagtg ataattacca accacaaaca tcatgttcgt tttccgtgtc ataagaacgc
     7381 acggtattca ccagatcttt tatcacttca gccgccactt ctggcaccag caaagtcatc
     7441 ggcgtctctg tttcataatc gacagaaacg ccattactgt tattggtgac ggtcacggta
     7501 tacgttgctt tgcccatgat tcatttcccg ttatgaatga ctttccgttg ttgcgcacct
     7561 tccatcagga cttcaggagc cacgaagaag tcaatgttga aataagtatc gtcagtcatg
     7621 gcttcaatgt tgtgccactt ttctggaggg aacacctcaa actgccccgc ttcgataagg
     7681 atcacctgat caggctctgc actgtgttca tcagcgtagc cgagatattt gaccgcccca
     7741 tgcataacgg aaaggcgtgg gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg
     7801 aatattccgg caggcgcagt ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa
     7861 ttctgtggga tttgcagcat ccttttccct ccttcggtga atgcgctgaa aacggtttat
     7921 tccagccgtt tcagggtacg cctgataatt tgcattttaa ataccattta ttggttactt
     7981 tttagcacca tatcagagaa aaatcaggga ggattacaag tgggaagccc gcgacaacgc
     8041 gggctgaatg tatcaggatt gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa
     8101 accaatcagt gagttgccaa ctttagccag tttctttttg gtacgtatgt actgcgtgac
     8161 aaaagcacca gaaataatca ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt
     8221 cgccagaata aagaatgaaa ttcccgtatg tggggcatta acatcgataa actgtacgaa
     8281 aaacgacaca tagaacaaaa tggctttcgg attagtcagg ctcaaaatta acgcgcgttt
     8341 aaaaatggcc ccgtattggg gctcatcgga tttggcctcg ttatttttac ccttcagggt
     8401 cgcgtagaga attttactcc ccagatagag caaataaaac gcaccaagat aacgtacaat
     8461 gttgaataat atcggggtgg tcttaattaa tgtcgccact ccagcccatg ccagaaacat
     8521 caatactgca tcgccaataa atacaccgca ggccgcaaga taaccgcctt tcataccgct
     8581 actgacgcta tttttgagta caaacagggt atttggccct ggcaccaaca caataaaaat
     8641 ggccccaacc agataggtcc agtaattcag aaccccgtat tcagcgaaca cattaacctc
     8701 tttaattatc tttcgatcat gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc
     8761 ttagaaagga gaaaatcagg ttttaacccg atatcaaccc gataattgaa taattaatca
     8821 ggcatgctcc agtgaaaaat tcgggtagtg ctcggcaaaa tactggcgta aaaattctat
     8881 cgttatccgc actttcgctg acgtcgccag ccttgaaaca taaacggccc agacgttcgc
     8941 tggctggtaa tattccggta gcacttgcac taaatgacca ctggcaatgt tttcgctaac
     9001 atcccaccag gagcgcagag caatcccctg cccatcgaga caccactgat ggacaatttc
     9061 tccatgatta gacgacagtg ggccggtgac tttaatcgca tggtggcctt ctttattgcg
     9121 taattgccag acaccaaaag gatgatcgcg ctctttaatg accaggcacg gcaatgcgga
     9181 aagatcggtt aaatgtttcg gcgcgccatg ttgcgcaata aattccggcg acgcgcagag
     9241 aatgcggtaa ttagtcgcca gtttgcgggc gattaaatta ggggcgatgt catcgccaat
     9301 gcgaatatcg agatcaacac cttcattgac caaatcgacc aaccggtctt ccacatcaaa
     9361 acgtaattcc agttgcggat acgccttagc cagcgctgat aacgccggag ccaccacctg
     9421 tcgtccaaaa ccaaagctgc tgataatacg cagcatcccc tgcggcacct gacgcacgtc
     9481 agaaagttcg tccatcatct gaccgacatc ctgcaaaatc cgctgcgccc attcataaat
     9541 ccgctctcct tcttcggtaa tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt
     9601 tagcgtttgc tccagcaagg cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc
     9661 ttccgccacg gcggcaaaac cagcccggcg agccaccagc ataaagacgc gcaaatcgtt
     9721 cagcagcggt aaattattca tgattcgtgt tttatgtttc accagttacg gggattaatt
     9781 ccttttcagt caattatagg atggtgatgt tgtcaatttt gatggtcagg aagtgagaac
     9841 ccaatgatga aaacgatgcg tattgctgcg atcccgggag atgggattgg caaagaagtc
     9901 cttcctgaag ggattcgcgt attacaggct gccgctgagc gctggggctt cgacttgagt
     9961 tttgagcaaa tggagtgggc gagctgcgag tattacagcc atcacggtaa aatgatgccg
    10021 gacgactggc atgagcaact tagccgtttc gacgccatct attttggtgc cgtcggctgg
    10081 ccggataccg ttccggacca tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa
    10141 ttcgaccagt acgtcaacct gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg
    10201 gcgggaaaac agcctggcga catcgatttt tacgtggtca gggaaaacac cgaaggcgaa
    10261 tattcctcgc tcggcggtag agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa
    10321 tcggtattta cgcgtcgtgg tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa
    10381 agccgcccac gtaagacgct aacttctgcc actaaatcaa acggtttagc catcagcatg
    10441 ccgtactggg atgagcgagt ggaagcaatg gccgagaatt acccggagat ccgctgggac
    10501 aagcagcata ttgatattct ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg
    10561 gtggtggcgt ccaatttgtt tggcgatatc ctttccgatc ttggcccggc ctgcaccggc
    10621 accattggca ttgccccatc cgccaacctg aatccggaac gcactttccc gtcgctcttc
    10681 gagcctgtcc acggttccgc gccggatatc tacgggaaaa atattgctaa ccctatcgcc
    10741 acaatttggg ccggtgcgat gatgctcgat tttctcggca atggcgatga gcgtttccag
    10801 caagcgcata acggtattct ggcagcgatt gaagaagtga ttgctcacgg gccgaaaaca
    10861 ccggatatga aaggcagtgc caccacgcca caggttgccg acgcgatttg caaaattatt
    10921 ttgcgttaag gtcaaaccag tttatttgaa ccgcgtcact gacgcggttt ttttattcgt
    10981 tctttgcagt taataacctg cgtcatttca ccttttattg tttccgtttc gtgttttatg
    11041 gctttccgta ttcttaattg tttaatttat gtaacatgca aataatcaat ttgttaaaca
    11101 caacatacaa ctcattaccc tgcaaataat tacaactggc aaaagccaga gacgggcttt
    11161 tatgcccagg agatgactca tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag
    11221 catcagcctg gtggccattc ttttcatcgc agctgcatta agccttttcc cacaacaatc
    11281 ggccgacgcg gccaacacaa tatacacttt tgttacgcgt acgttaggtt ccgccgtaca
    11341 ggtattggtt ttgctggcaa tgggactggt gatttattta gccaccagta aatacggcaa
    11401 tattcgtctt ggcgaaggaa aaccggaata cagcacgctc tcctggctgt ttatgtttat
    11461 ttgtgccggt ttaggttctt ctacgcttta ttggggggtt gctgaatggg cctattatta
    11521 tcaaacgcct ggattaaata tcgcaccacg ttcacaacag gcactcgaat ttagcgtccc
    11581 ttactccttc ttccactggg gcatcagcac ctgggcaact tatacgctgg cctcattaat
    11641 catggcttat cactttcatg tgcgtaaaaa caaaggtctg agtctttccg gcattattgc
    11701 cgcgattacc ggcgttcgcc cgcaaggccc atggggaaaa ctggttgatt tgatgtttct
    11761 gatcgccact gtcggcgcac tgactatttc ccttgttgtc accgcagcaa cctttactcg
    11821 tggactttcc gcgctgaccg gtttacccga taacttcacc gtgcaggcat ttgtgatcct
    11881 gctttccggc ggcatttttt gcctaagctc gtggattggt atcaacaacg gtttgcaacg
    11941 tctgagcaaa atggttggct ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg
    12001 tccaaccgaa tttattacca acagcatcat caatgccatc ggcctgacca cgcaaaactt
    12061 cctgcaaatg agcttattca ccgatccgct tggcgatggt tcatttaccc gcaactggac
    12121 cgttttctac tggctgtggt ggatctcata cacccctggc gtagcaatgt ttgtcacccg
    12181 cgtttcccgc ggtcgtaaga ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt
    12241 cggttgctgg ttcttcttcg gcgtaatgga aagctatgcc attcatcagt ttatcaatgg
    12301 cgtaatcaac gtcccacagg tgctggaaac actgggcggc gaaacagctg tacagcaagt
    12361 tctgatgtcg ttgccagccg gtaaattgtt cctcgccgca tacctgggcg tgatgattat
    12421 tttccttgcc tcgcatatgg atgcagtggc ctacaccatg gcggcgacca gtacgcgtaa
    12481 tctccaggaa ggtgacgatc ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac
    12541 tctgatcccg ctttccatct tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt
    12601 cgtgctcaca gcccttccct tcctcgtcat tttactggtg aaagtcggcg gatttattcg
    12661 ctggctgaaa caggattatg ccgacattcc ggctcatcaa gttgaacatt atctcccgca
    12721 gacaccggtt gaagccctgg aaaaaacacc agtgctccct gcgggaaccg tattcaaagg
    12781 cgacaactga gcgccatcat cctaacgata aaggtatccc tatgagcaat ctgagccctg
    12841 actttgtact acccgaaaat ttttgcgcta acccgcaaga ggcgtggacc attcctgccc
    12901 gtttttatac cgatcagaac gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct
    12961 ggatttgcgt cgctcacagc agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga
    13021 tcattggcga aagcatcgtg ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata
    13081 acgtgtgtcc gcaccgtggt catcagttgt tgagcggtga aggaaaagca aaaaatgtga
    13141 ttacctgccc atatcacgca tgggcattca aactcgatgg caatctggcc catgcacgta
    13201 actgcgaaaa cgtcgccaat ttcgatagcg acaaagcgca actggttccg gtgcgtctgg
    13261 aagaatatgc cggattcgtc ttcatcaaca tggaccccaa cgccaccagc gtagaagatc
    13321 agttacccgg tctgggcgcg aaagtgctgg aagcctgccc ggaagtccac gatctgaaac
    13381 tggcggcccg ctttaccacc cgcacgcctg ccaactggaa gaacattgtc gataactatc
    13441 tcgagtgcta tcactgtggt ccggcgcatc caggtttctc cgactccgta caggttgatc
    13501 gctactggca caccatgcac ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg
    13561 aacagtcgtt taaatttgaa gagggtacgg atgcggcatt ccacggtttc tggctgtggc
    13621 cgtgcacgat gctgaacgtc accccgatca aagggatgat gacggtcatt tatgaattcc
    13681 cggtggattc tgaaactacc ctgcaaaact acgatattta cttcaccaat gaagagttaa
    13741 ccgacgagca aaaatcgctg attgagtggt atcgcgatgt gttccgtccg gaagatttac
    13801 gtctggttga aagcgtacag aaagggctga aatcgcgtgg ctatcgtggt caggggcgca
    13861 tcatggccga cagtagcggt agtggcattt ccgaacatgg tatcgcccat ttccataatc
    13921 tgctggcgca ggtgtttaag gactaatgac atcggcggcg gtattttccg ccgctgggct
    13981 gatttttgat ggagtacagc aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag
    14041 gtggaaccgc ttaccgaaca ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg
    14101 ctgcctgcgt tcaccggagg aagtcacatc attgtgcaga tgagcgatgg tgataaccag
    14161 tacagcaatg cgtattcact actgagttcg ccgcatgaca cctcttgtta tcagattgcc
    14221 gttcggctgg aggaaaactc gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg
    14281 ggcgatcggt taacgatttc aacgcctaat aacctgtttg cgctaattcc ctcagccaga
    14341 aagcatctgt ttatcgcggg cggtattagt atcacccctt tcctgtcgca catggcagag
    14401 ttgcaacaca gcgacgtcga ctggcagcta cattactgct cgcgaaatcc agaaagttgc
    14461 gcatttcgtg atgagctagt ccagcatccg caggctgaga aagtccattt gcatcattca
    14521 tcaaccggaa cacgactgga attagcgcga ttattggcgg atatcgaacc tggcacacac
    14581 gtttatacct gtggccccga ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg
    14641 gacatcgccg ccgatacgct gcactttgag caatttgcta tcgaagacaa aaccggcgat
    14701 gcctttaccc tggtgcttgc ccgttccgga aaagagtttg tggtgccgga agagatgact
    14761 attttgcagg ttattgaaaa taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg
    14821 gtatgcggaa cctgcgaaac agcaatactg gaaggtgaag ctgaccatcg ggatcagtat
    14881 tttagcgatg aagagcgtgc cagccagcaa agtatgttga tctgttgttc gcgtgcgaag
    14941 ggtaaacgcc tggtgttgga tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc
    15001 cagtccggct tcggaagatt ttactgcgga tattcctgca ataaattgtg taatgcttcc
    15061 gccatcagct caccacgcca gccggaaatc agctccggca aattgttctg cggtttcagt
    15121 ttccagtgcc agttcagcag ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg
    15181 atcttatgcg tttcgctcac gtcagtaatc agcgacttaa tcgctttaaa cgctttacga
    15241 taacccggca tgtccatcag gttaagcatc ggctgcggta aggcctcttc cggcaatgtc
    15301 tgcgcttttt ccaccagcgc cagcagcgtt ttaccgtgaa agcggatttc gctaccggat
    15361 aaacccaggc tgtccagttc gcctaaactt cccggcatat aacgcgcgac cgaccacaaa
    15421 tgctcttcac gcacgacaaa gttcaccgcc agatcacgct cacgagcctt gcgcagtcgc
    15481 cagtcggcta acagttgcag acaggccagt tggcgtgtgc gtaattgcca ggcattggtg
    15541 atatcacgcc aggcatcttc cggcgcaacg acttcctgac gacgcatttg catcaggcgg
    15601 cattcatcca gcgccgcagg tagccagccg gaggcctccg tttctaccat aagcttggcg
    15661 gtgatcggta acagatacca gacatccgcc gctgcgtatt cacactgacg ttcggtcagc
    15721 ggtctggcca gccagtcggt gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct
    15781 tccaccatgg aagcgaaacc ccatgacatc gggcgtccgc agaaggcagc aaggatttgc
    15841 gtgtcaatca ggggttgtgg taattcgcca aagacattga ggaacacttc cagatcttca
    15901 ctgcctgcat ggagaaattt tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac
    15961 cagtcggtga tcccgagagg atcaattagc gccagatgct caccatcgaa aagttgaatc
    16021 aaccccagct gcgggtaata agtgcgcgta cgaacaaatt cagtatcctg ggctatcgcc
    16081 ggaaagacac ggacggcttc acacaaagaa gccagcgcat cgtccgtggt aatcatttgg
    16141 taattcaaat tgtattctct ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct
    16201 gacgactgac ttaacgctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat
    16261 tctcgtcgca aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc
    16321 ttcggtactt tgtagcccgt gagctgacgg cagcaaaagg tcaccagtga ctcttcggta
    16381 agcgatggat cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt
    16441 acgccaacag ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg
    16501 ttgggataga cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc
    16561 aggaaccctt cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg
    16621 atctcatcgg tagcatcggg acgctgccag taacccagca tcacctgcgg tcctttgaca
    16681 caaagctcac ccggttgacc cggtggtact tcattatcgt cgtcatccac cagtttggct
    16741 tccgtcgacg gcaccggcaa accgatgcta ccactatgat aatcaatatc atatgggtta
    16801 acgctgacca gcggcgcaca ctcggtaaga ccatagcctt ccagcagata ctgtcctgtc
    16861 agtttcaccc aacgctctgc caccacttgc tgcactggca tcccaccgcc tgcggaaaga
    16921 tgcagactgg agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacaag
    16981 gtgttaacgc cagtgatagc ggtaaacgga tatttcgcta actcttttac caaccctgga
    17041 atatcgcgcg ggttagtgat aagcaggttc tgcccaccca gttcgataaa cagcaggcag
    17101 ttaatggtca gggcaaaaat gtgatacagc ggcagtgccg ttactaccag ttctttacct
    17161 ggatgcagca acggcccata ggtggcgttg acctgttcca ggttcgccag catattgcgg
    17221 tgagtcagca tcgcgccttt cgccacacct gtggtgccgc cggtgtattg caggaaggct
    17281 aaatcttccg gcaccagttc gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg
    17341 ctacgaaatg aaatggcatc tggcagatgg tatttcggca ccaaacgctt gatgtattta
    17401 acaacgaaat tgactaccgt gccttttgcc gtagatagct gatcgcccat acgggtcaga
    17461 attacgtgct gaacggcggt tttatcaacc actttttcca gtgtgtgagc aaagttagac
    17521 acgataacaa tcgccgatgc gccgctatcg ttaagctgat gctcaagctc acgcggggta
    17581 tacaacgggt taacgtttac gacgatcatc ccggcacgca aaatgccaaa cagcgccacc
    17641 ggatattgca ataaattagg catcatcaac gcaacgcgat cgcctttctt cagccccaac
    17701 ccttgttgca aataagcggc aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt
    17761 acctccccca tattcacaaa cgcaggctga tcggcgtagc ggacgaccga ctgctcaaac
    17821 atatctatca gagattgata acggtcaggg ttgatctccg tcggaacatc cgcgggataa
    17881 cggttaagcc aaaccttctt caattcttca cctctaaaat gcgtgttcgt cgtcatcgca
    17941 accccaagtg ataaacatgt tgttaacata acattaactc atcttaccag cttgataatt
    18001 agacaacgaa aaggttgcga agcgcgtcac tatttatttt tatagttgcc gtatgaacgc
    18061 agaaacagcg gaccagccgc tgtttctttt tctttaaaaa caacttgtta ctcggttaca
    18121 attgtttcaa ctcttgcagg gccaggatta taccaacccc atccgccgta accatagggc
    18181 cagccgcgtc caccataaaa ccatggatca attggctgcg gcggcataat cacctgttga
    18241 gtgatatgcc agcgtttgta acctgttacc tgcataacca taaatttata tggtgcatta
    18301 tttactttgc catccactgt gccagtgatt ggcccgacaa ccgtcaccag ttgtccacga
    18361 aaatcgaccg gatcaagaaa accgttcaca tcggcataaa tgcgcccgcg tgaaggttct
    18421 cccaaggttg gtcttgctcc gctgtccagc ggtacggtag caatttccag gcgggttttc
    18481 ccttgctggt tttgtaccac aaccactttg ccgccaaagc gcgcctcctg accaacatat
    18541 aaatctggcg cattcatcac gcgtaccaga tcttgctgtg gtgttggact gctgccttta
    18601 atggcgtccg gcacagtgac acaaccgctc agcattagcg caaacgtacc tgccagtatg
    18661 cctttgataa cattcttttg aaccgccatg atgcgactcc tttttttctc agggcatact
    18721 cttaagattc attctttgcc cggaagtttc ttccatgcga cgttgttacg taaataaacc
    18781 ggttcggcat gttccaccgc caccgtttta ccctcggcaa acatctgaca cgcaatcggc
    18841 agcatatctt ccgccgctgg cagtaaaact tctccgtcgc gcaaaaccag cccgctctct
    18901 ttacggagat ccggccaggc ttgccagccc gtccctaccg ttacccattc gccggaaagc
    18961 tgttgcattc gttcatgaac gagttcgggt ttgagtacgg cttcggtttc ttcaccgcgc
    19021 cagataccgt tttcatcacg ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca
    19081 atggctgcca gcacgcgggt tgcgccgttt ttgcgccacg ccccctgcgc catcgtcatt
    19141 agcgtagaga caccaatcat cggtaactcc gcgccaagcg ccagcccttg cgcaatacca
    19201 ataccaatgc gcacgccagt aaagctaccg gggccgcgcc cgtaagccag agcgtttatg
    19261 tcagtcaggg aagttccgct ggtggtcagg atatcctgca ccatcggtaa gattcgttga
    19321 gtatgttcac gagggcaaag ctcaaaatga gcgttgacat taccgtcgtt ccacagagcg
    19381 acagagcaga cctctgtcgc ggtatcgata gccagaattc gcatgggtct tcgtgcttag
    19441 atcaataaaa aggcgcgcat cataccatac tccgtaacaa attacctgga ggatggtatc
    19501 gcacaaggaa acgaaccgca cgggcaatgt cacgggtgcg tggcgcgggc ggcagactgg
    19561 cgagaaacgt cgcgccgtaa ggacgcatca ccagccgatt gtcacaaatt accagcacgc
    19621 cgcgatcgtc ggcgtcgcga atcagtcgcc ctactccctg cttgagagta atgaccgcat
    19681 ccggcagctg cacttcatcg aacgggtcgc caccgcgcaa acgacaatct tccatgcgcg
    19741 cttttaacag tagatcatcc ggcgaggtaa acggcaattt gtcgataatt accaatgaca
    19801 atgtatcgcc acgcacgtcc accccttccc agaaactact ggttgccaca agaagcgcgt
    19861 taccggcgct gacaaattgc tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa
    19921 cgggaagcgt catggtagcg cggaactgct cggccagatc gcgcatcatg gcatgcgagg
    19981 tacaaagcat aaaacaacga ccgttgttag cttcgatgat cggtcgcagc attgccgcca
    20041 gttgccgtgc acaacccggt tgattggttt gcggcaggtt gcgcggcaca cagagtaacg
    20101 cctggcggct gtaatcaaac gggcttggca atagcaacga ctccgcctgt tctatcccaa
    20161 gccgcgaggt gaaatgatgc agatcgtcgt tcaccgacag cgttgctgag gtgaagatcc
    20221 agctaccggg cttttgcgcc attaactctt tgaatttatc cgccacgctg agcggcgtga
    20281 gagccagagt aaaatggcgc gaagtgcatt cgtaccagta gctgtagccc ggctgattga
    20341 tctcttttag ccgcttcagc cgtgtgcgat acaacgtggc gcgctcaaat gccgcatcca
    20401 gcaaggcgga acgccccagt gacagtttcg ccacgtcata acaaagttcc agggtgtcat
    20461 cgagcagtaa aaatgcccgc tgaatttgcg gattagctaa cagctcacgc aggttaccgc
    20521 gataacctgg ctcaccgagt tgcagacgaa aatcctgtgc actctgggca agacgatcag
    20581 cgcacttttg taactgctgg gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt
    20641 ttgccaggtc gagcagttgt cgactggaga gtgactgacc aaaatactgg ctggcaatgt
    20701 ccggtagctg gtgggcttcg tcgaagatca tgacgtccgc ttcagggatc aactcgccaa
    20761 atccactctc tttaaccacc atatccgcca gaaagagatg atggtttacc accaccacat
    20821 cggcgtccat cgctttttta cgtgctttga ccacaaagca atctttatac atcgggcagt
    20881 cgctgccaag acagttgtcg ttggtgctgg tgaccagcgg ccacgcctgt gaatcttccg
    20941 ccacgctgac gcaggtgctg atatcaccat cgactgtttg attagaccag gagcgcagca
    21001 ggatcacatc gcttaagatt tgtaccggca gatcgccccc cgccagcgcc tgctgttcga
    21061 gacgttcgag gcagaggtag tttgagcgcc ctttcagcag cgccacgttg cccgtatatt
    21121 tcaatgcctt tgagactgtt ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg
    21181 agccggtcga gataatgact ttctttttcg cccgcagcgc aggagccagg taagcgtagg
    21241 ttttgcccgt accggttcct gcttccacca ccagcggctg gcctttttct atcgcctggg
    21301 tgacggctac cgccatctgt cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg
    21361 ccagctgacc gtctggtgca aaatcgtccg tcacactacc ccctgttgat ttgaacaggg
    21421 attatgtcag gatgagggcg ctttcgccag ttgaagtggt gacggcaacc tcacattgtg
    21481 gcagtctttg cagcacgaaa atggaagtat aatgaggaca aaatgatgac tatcgttcgt
    21541 atcgatgctg aagcccgctg gtctgatgta gtaatccaca acaacacgct ctactacact
    21601 ggtgtaccgg aaaacctcga cgccgatgcc tttgagcaaa ccgccaacac gctggcacag
    21661 attgacgccg tgctggaaaa acagggcagc aataaatcga gcattctgga tgccaccatt
    21721 ttcctggccg ataaaaacga cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc
    21781 gcgggtcatg cgccagtgcg ctgcacggta caagcgggtt tgatgaaccc gaagtataaa
    21841 gttgaaatta agattgtggc tgcggtgtaa gctttatcga agcaaaataa gtcagacgat
    21901 aatttatcga taatactggt cggttttaca taaatcgacc agagaataag atattactgt
    21961 tgatgcttct gctaattcat tatt
//
LOCUS       DAZOZY010000051        21642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZOZY010000051 DAZOZY010000000
VERSION     DAZOZY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.051"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHURQ_001713"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHURQ_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR0823516.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="ACHURQ_001714"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="ACHURQ_001714"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR0823517.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHURQ_001715"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHURQ_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR0823518.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="ACHURQ_001716"
     CDS             complement(3716..4510)
                     /locus_tag="ACHURQ_001716"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR0823519.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="ACHURQ_001717"
     CDS             4648..4989
                     /locus_tag="ACHURQ_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR0823520.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHURQ_001718"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHURQ_001718"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR0823521.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="ACHURQ_001719"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="ACHURQ_001719"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR0823522.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="ACHURQ_001720"
     CDS             8905..10197
                     /locus_tag="ACHURQ_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR0823523.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10228..10325
                     /locus_tag="ACHURQ_001721"
                     /pseudo
     CDS             10228..10325
                     /locus_tag="ACHURQ_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="ACHURQ_001722"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="ACHURQ_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR0823524.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="ACHURQ_001723"
     CDS             complement(10730..11188)
                     /locus_tag="ACHURQ_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR0823525.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="ACHURQ_001724"
     CDS             complement(11185..12102)
                     /locus_tag="ACHURQ_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR0823526.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="ACHURQ_001725"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="ACHURQ_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR0823527.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="ACHURQ_001726"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="ACHURQ_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR0823528.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHURQ_001727"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHURQ_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR0823529.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHURQ_001728"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHURQ_001728"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0823530.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHURQ_001729"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHURQ_001729"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR0823531.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHURQ_001730"
     CDS             16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHURQ_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR0823532.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHURQ_001731"
     CDS             16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHURQ_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR0823533.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHURQ_001732"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHURQ_001732"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR0823534.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHURQ_001733"
                     /pseudo
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHURQ_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5685 c         5799 g         5144 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg acatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccatctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgttgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtggcga tcctcaaaac
    17581 gctaggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctagcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag at
//
LOCUS       DAZOZY010000052        21454 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZOZY010000052 DAZOZY010000000
VERSION     DAZOZY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21454
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.052"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(63..971)
                     /gene="galU"
                     /locus_tag="ACHURQ_001734"
     CDS             complement(63..971)
                     /gene="galU"
                     /locus_tag="ACHURQ_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR0823535.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1173..2186)
                     /gene="rssB"
                     /locus_tag="ACHURQ_001735"
     CDS             complement(1173..2186)
                     /gene="rssB"
                     /locus_tag="ACHURQ_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR0823536.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2278..3183)
                     /gene="rssA"
                     /locus_tag="ACHURQ_001736"
     CDS             complement(2278..3183)
                     /gene="rssA"
                     /locus_tag="ACHURQ_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR0823537.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3296..3754
                     /locus_tag="ACHURQ_001737"
     CDS             3296..3754
                     /locus_tag="ACHURQ_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR0823538.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3804..4646
                     /gene="purU"
                     /locus_tag="ACHURQ_001738"
     CDS             3804..4646
                     /gene="purU"
                     /locus_tag="ACHURQ_001738"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR0823539.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4806..4890
                     /locus_tag="ACHURQ_001739"
     tRNA            4806..4890
                     /locus_tag="ACHURQ_001739"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4840..4842,aa:Tyr,seq:gta)
     gene            4938..5069
                     /locus_tag="ACHURQ_001740"
     ncRNA           4938..5069
                     /ncRNA_class="other"
                     /locus_tag="ACHURQ_001740"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5253..5930)
                     /gene="narI"
                     /locus_tag="ACHURQ_001741"
     CDS             complement(5253..5930)
                     /gene="narI"
                     /locus_tag="ACHURQ_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0823540.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5930..6640)
                     /gene="narJ"
                     /locus_tag="ACHURQ_001742"
     CDS             complement(5930..6640)
                     /gene="narJ"
                     /locus_tag="ACHURQ_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0823541.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6637..8175)
                     /gene="narH"
                     /locus_tag="ACHURQ_001743"
     CDS             complement(6637..8175)
                     /gene="narH"
                     /locus_tag="ACHURQ_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0823542.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(8172..11915)
                     /locus_tag="ACHURQ_001744"
     CDS             complement(8172..11915)
                     /locus_tag="ACHURQ_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR0823543.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(12308..13699)
                     /gene="narK"
                     /locus_tag="ACHURQ_001745"
     CDS             complement(12308..13699)
                     /gene="narK"
                     /locus_tag="ACHURQ_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR0823544.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            14038..15834
                     /gene="narX"
                     /locus_tag="ACHURQ_001746"
     CDS             14038..15834
                     /gene="narX"
                     /locus_tag="ACHURQ_001746"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR0823545.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            15827..16477
                     /gene="narL"
                     /locus_tag="ACHURQ_001747"
     CDS             15827..16477
                     /gene="narL"
                     /locus_tag="ACHURQ_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR0823546.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(16478..17872)
                     /gene="ychO"
                     /locus_tag="ACHURQ_001748"
                     /gene_synonym="iatA"
     CDS             complement(16478..17872)
                     /gene="ychO"
                     /locus_tag="ACHURQ_001748"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR0823547.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            18057..18410
                     /gene="ychN"
                     /locus_tag="ACHURQ_001749"
     CDS             18057..18410
                     /gene="ychN"
                     /locus_tag="ACHURQ_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR0823548.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(18454..19149)
                     /gene="chaC"
                     /locus_tag="ACHURQ_001750"
     CDS             complement(18454..19149)
                     /gene="chaC"
                     /locus_tag="ACHURQ_001750"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR0823549.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(19307..19537)
                     /gene="chaB"
                     /locus_tag="ACHURQ_001751"
     CDS             complement(19307..19537)
                     /gene="chaB"
                     /locus_tag="ACHURQ_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR0823550.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            19807..20907
                     /gene="chaA"
                     /locus_tag="ACHURQ_001752"
     CDS             19807..20907
                     /gene="chaA"
                     /locus_tag="ACHURQ_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR0823551.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            21310..21417
                     /locus_tag="ACHURQ_001753"
     CDS             21310..21417
                     /locus_tag="ACHURQ_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0823552.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5273 a         5685 c         5250 g         5246 t
ORIGIN      
        1 aagctataaa aaaacggcgt cgattgctca acgccgtttc gtggataaca ccgatacgga
       61 tgttacttct taatgcccat ctcttcttca agccaggctt taaattccgt gccaagggtg
      121 ttatgacgaa taccgtattc aacgaaggcc tgcatgtaac ctaatttatt accgcagtca
      181 tggctcttcc ctttcatatg ataggcttcc accgtttctt tttcgatcag catatcaatt
      241 gcgtcggtga gctgaatttc atcaccagct cccggagggg tttttgccag caacggccaa
      301 atatccgcgc taagtacgta acgacccaca atagcgagat tagacggcgc aacatccgct
      361 ttcggttttt ctaccacacc aaccatcggt acgctttcac ccggcgctaa ttcaacgcct
      421 ttgcaatcca caacgccata tgcggtcaca tcagcaaccg gttcaaccat gatctggcta
      481 tgacccgttt catcaaagcg gcggatcatc tctgccaggt tatcctgtga caaatcggat
      541 tcatattcat ccagaataac atcaggcaaa ataacagcta ccggttcatc acccactacc
      601 gggtgagcac acaataccgc gtgtcccagg cctttcgcca gaccctgacg aacttgcata
      661 atagtcacgt gcggtggaca aatagactgc acttcatcaa gcagttgacg ttttacacgt
      721 ttttccagca ttgcttccag ttcaaaactg gtatcaaagt ggttttcaat agagttttta
      781 gatgagtgtg taaccagcac aatttcagta atgccagccg caatacattc attcacgacg
      841 tattgaatta atggcttatc gacaagtggc agcatctctt tcgggatggc tttcgtcgcc
      901 ggcaacatcc tggttcctaa tcccgcaacg gggataacgg cttttttgac tttcgtatta
      961 atggcagcca tttaaattct cctggactgt tcgtgttttg aacgtgttca tatttctgta
     1021 tcgcatccca gtatatcagc accccgaagg ggctaagttc tgaaaaggag gcgagttagc
     1081 ataattaaga caattactta tacgaatggc gacgatagta ccaccagccg acattagcag
     1141 gtaatgcaaa tttagcccgc gttatcgttt gctcattctg cagacaacat caagcgcagt
     1201 cgaccaccgg ttccccatat ttggcattgc caggcatcgc atcgctggct caattgattc
     1261 aaataagcgt tacctaaagt gcctaacgga acaccattac tgatttgcac ctggtgttcg
     1321 ccggtattta acgtcgcatt cagacccgca gaaaccagaa tgagattttt cagttcgcga
     1381 tgataatagc caactaatag cggaaactgc cccggcagat tggcctgacg gagtaaatgg
     1441 tttacctgct tcaataacgc gcccaactct ggcaaccgtt gattttggtg tgcaagctgt
     1501 tcctgtaata atccgttaaa caatgcgcgt aataacaagg cagcaagtac gccattatgt
     1561 ccagctcggg tgacatcaag gcaataaaat gccagatcgt tttccgaaag tgcggcaata
     1621 tcaagcacca ggccgggttt gtccgcggca accaattgac gataattaac ccggcaatgg
     1681 gaaatcacct gctgaaccgg cggttgtagt tcctgtaata atttcgccgc tgcggcaggg
     1741 ttatcaacca ttgcatccca gtcgcgaaaa agcctttcct cttcctcaac gcgcgaatta
     1801 aacatgctgg gatagagaca ggcaaaaacc atctcgcgca agcgattcag atctttaact
     1861 ggtttcagca aaacatcttc aacgcccaga cgtaacgctt tggcaatatc tgccatattt
     1921 tcagtggcag atatcaccag aactggggtc tggtcgcctc tgttacgtat atgctccagc
     1981 agtttaagcc cgttcattcg tggcatcgcg atatcacata tcatcaggtc tggagtgaaa
     2041 cctcccagca actcaagggc atccacccca tcagccgcca gtaccgttgt cgctcccaat
     2101 gaggaaaacc atgaatccag aagcgagcga aatacctgct catcttcaac aatgagaatc
     2161 tgttttccga ccaatggctg cgtcatgttc tctcccctga ctggctttac tcaatagtgg
     2221 catgctattg ccacctgcgc ctgtcagaat taacttaagt gtagataaaa attctggtca
     2281 aatgttggtg cgtaccaacg gcaaaagttc gtccattttc ctttccactg ccagctgtcc
     2341 ggccgcaatg gcagcgtgcg cgcgatggaa atcaagcgta gatatttgcg ggcaaacagg
     2401 ttgaatcaga atatcgggcg gatcacctgc catgcggttc cttttaaggc ggttctccaa
     2461 cacctggata gacgtggtca taatctctgt tgccgttggc gctgtcacag cccgacgtgt
     2521 cgttatgctg cctaaccttt ctttcagacg cgcatgccac ggtagagaat caccattctc
     2581 gctattttct tcactgacat taaaggagag cagatcttgt tgcatcaaat gagcatcgtg
     2641 ctgcaggtcg accgctatca caatatcagc ccccaatgca cgcgtaagag aaataggaat
     2701 tgggttaact actgccccat caaccagcca gtagccgtta tgtgcaacgg gtgccatgag
     2761 tcctggaata ctgcatgatg cgcgaatagc aagatggaga tcgccttcag taaaccataa
     2821 ttcacgcccc gtacttaaat tggtggcaac agccgcaaag cgacgggaac aattttcgat
     2881 ctctgtttcc ggcattattt cgcgatattg attgaagaca cgctcgccgc gcagtaaccc
     2941 accgcgctgc caggagaggt ccatcaggcg taaaacgtcc caataactga aagaggttac
     3001 ccaatcttcc agcgcagata atcgatcgca tgcataggcc gcgcccacca gcgaaccaat
     3061 tgaacatcct gcaacgatat caatttcaat acccactttt tttagcgcat taataacgcc
     3121 aatatgcgac caacctctcg ccgcgccaga tcccagcgcc agccctattt ttatctttct
     3181 cattattccc gcaagattcc cctggaatgc aatcgtagcc acatcgctac tgctcggtta
     3241 acatagtgct actcaggcgg ttaatacgcc gttattgttt ccagggagat tatttgtgtc
     3301 tcagctttgt ccctgtggta gtgctgtcga gtatagccta tgttgccacc cttatgtgtc
     3361 tggtgaaaag gttgcacctg atccagaaca tctcatgcgt tcgcgttact gcgcttttgt
     3421 gatgcaagac gcagattatt taataaagac ctggcatccc tcttgtggag cagcggcgtt
     3481 acgtgccgaa ttgatggccg gatttgcgca tactgaatgg ttgggattga ccgtatttga
     3541 acattgttgg caggacgccg acaatatcgg atttgtcagt tttgttgctc gatttaccga
     3601 aggtggcaaa acaggtgcaa taattgaacg ttcgcgattt ttgaaagaaa acggtcaatg
     3661 gtactatatt gacggtacac gtccgcagtt tggtcgcaac gatccctgcc cttgtggttc
     3721 aggtaaaaaa tttaaaaagt gctgcggcca ataatggttg acggtacggt ttagcaaaca
     3781 ctctcaacaa ggtttttcca gcaatgcatt cactccaacg taaagttctg cgtactattt
     3841 gtccggacca aaaaggtctg atcgcacgta ttaccaatat ttgctacaag cacgagttaa
     3901 atatcgtaca gaacaatgaa tttgttgatc accgtaccgg gcgctttttt atgcgcacgg
     3961 aactggaagg gatttttaat gattccaccc tgctggcgga tctcgatagc gcattgccag
     4021 aaggctccgt gcgtgagctg aatcctgccg gtcgtcgccg gatagtgatt ctggtcacta
     4081 aagaagcgca ttgccttggc gatttgttga tgaaagccaa ttacggcggc ctggatgtcg
     4141 aaatcgcggc agttattggt aaccacgata ctttacgttc tctggttgag cgttttgata
     4201 ttccgtttga gctggtaagc catgaagggt taagccgcaa cgagcacgat caaaagatgg
     4261 cggaagccat tgatgcttat caacctgact atgtggtgct ggcgaagtat atgcgggtat
     4321 taacgccgga atttgtggca cgcttcccga ataaaatcat caatattcac cattcattcc
     4381 tgcctgcgtt tatcggcgca cgtccttatc accaggccta tgaacgtggt gtgaagatta
     4441 ttggcgcaac cgctcactat gtgaatgaca atctggacga aggcccaatc atcatgcagg
     4501 acgttattca tgtcgatcat acctacacag ctgaagatat gatgcgcgca ggtcgtgacg
     4561 tcgagaaaaa cgtcttaagt cgtgcgctat acaaagtact ggcacagcgc gtctttgttt
     4621 acggtaatcg gacgattatt ctttaatcgc cagcaaaaat aactggttac ctttaatccg
     4681 ttacggatga aaattacgca accagttcat ttttctcaac gtaacacttt acagcggcgc
     4741 gtcatttgat atgatgcgcc ccgcttcccg ataagggagc aggccagtaa aaagcattac
     4801 cccgtggtgg ggttcccgag cggccaaagg gagcagactg taaatctgcc gtcatcgact
     4861 tcgaaggttc gaatccttcc cccaccacca tcacttttca aaagtccgta aactctcaaa
     4921 cgaatccgca attaaatatt ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt
     4981 cgacttgagc gccagcgaga gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc
     5041 agcgctgagt aatccttccc ccaccaccat cgcttcctta aataatcaaa acctgttact
     5101 ccgagctaat tttgcccgat ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag
     5161 gtcggataag cgaagcgcat ccgacaactc accccgcacc gtgcccataa aaaaaccccg
     5221 ccgaagcagg gtctatgtga actaaaattc gcttagtgac gagcgcgcac cagctggtac
     5281 ttacgtgtca gatactccac cggtacgctc cagatgtgta ccagacgcga gaacgggaac
     5341 agcaggaata acgtcatccc cagcaccagg tgcagacgga agataaacgc cacaccatcg
     5401 aggtgttgag aagcgccacc gtggaaggtc accaccgact gcgcccagcc aaccagtttc
     5461 atcatctcgc taccgtccat atgctgagcg gagaacggaa tggtcaacag gcccagcgcg
     5521 cactggataa cgagcagcga caggatcagg atatccgctc cggtagtggt tgcacgcacg
     5581 cgtgggctga acagacgacg cttcagcaac agcacgccgc caatcagaca cagcacgccg
     5641 ctggcaccac cagcaaacat cgccattttc tgtttcactt caatcggcag ccaggcttca
     5701 tacatccagt gcggcgtcag cataccgaag aagtgaccaa caaaaatccc cagaataccg
     5761 atatggaaca ggttcgacgc caggttcatc cctttgcgat ccagcatctg gctggacgcc
     5821 gcgcgccagg tgtactgccc gtagtcataa cgcagccagc taccaatcag gaagaccgcc
     5881 ccggctatgt acgggtagat atcaaagaag aacatattca ggaattgcat tagtgctgtc
     5941 ctccggtggt gatattcaga tattgcggcg cgacggcacc ggcaaagcga cgctgatgag
     6001 cagtgattgc tgaatcaccg cagcctttgt cagcaaagaa tttaacctgc tcttcttccc
     6061 agacagcatc cagcgcctgc ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca
     6121 ctttgtcgct gtcgatagcg gtattggcca atttcagcag cagatcaaac atcacggcat
     6181 aacggctttc acgctgttgc agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt
     6241 tcaaaccttc cacggcttcg ctttgcggca gctgcgacag gtactccaga tacagcggca
     6301 gatggtccgg taattcgcgg ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca
     6361 ggtccaccat cgcctggccg cggtcacggg attcaccgtg aacatgttca aatagcaaca
     6421 gtgacgtggc gcggccacgg tcgaacagtt cgctgtactg ggcctgggca tcgagcggat
     6481 ccatcgccgt taaatcgcgc aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat
     6541 ttttcgacgc agcaatcgcc tcaaacatct cttgttgatg ctgccataag gcagcatccg
     6601 gatattcaag gagacgcgat acaatcacga gttcgatcat ggatgcggct ccgttttgct
     6661 ggtcacatcg atggcatcaa tacgacggct gttgaacaga ttgaatttgg tatctgaacc
     6721 gtggcagcca tcaccaaagg taaagccgca gccatttttc tccgggaagg cttcccgtgc
     6781 cagttcacga tgactactcg gcaccacaaa gcgatcttcg tagttagcaa tcgccagata
     6841 acggtacatc tcctgtgcct gggcttcggt cagaccgacc tcttccagcg cacgggtatc
     6901 aactttaccg tcaacggttt cagcacgttt gtaatgacgc atcgccagca tacgtttcag
     6961 tgcgcgcagt accggtttgg tatcaccggc ggtcagcaga ttcgccagat actgtaccgg
     7021 aatacgcaag ctttcgacgt ctggcagaat gccgttgcta cccagctcac ccgcgtctgc
     7081 tgcagactga atcggagaca gaggcggcac gtaccagacc atcggcagtg tgcgatattc
     7141 tggatgcagc ggcagcgcca gtttccattc cattgccatt ttataaaccg gcgactgctg
     7201 tgcggcttca ataacgctca gcggaatacc gtctttaatc gcctgctcga tgactttcgg
     7261 atcgttcgga tcgaggaaca cctccagctg acgctggtaa agatctttct cgttctcggt
     7321 gctggctgca cgttcaatcg cgtcggcatc gtacagcagc acgccaagat aacggatacg
     7381 accgacacag gtttctgagc acacggtcgg ctgacccgct tcaatacgcg gatagcagaa
     7441 gatgcacttc tcagacttac cgctcttcca gttgaagtag atttttttgt acgggcatcc
     7501 ggtgatgcac atacgccagc cacggcattt atcctggtcg atcaggacga tgccatcttc
     7561 ttcacgcttg taaatcgcac cgctcgggca ggtcgccaca catgccgggt tcaggcagtg
     7621 ttcgcacagg cgcggcaaat acatcataaa ggtgttttcg aactggctat acatcgcctt
     7681 ctggatgttg tcgaagttct tgtctttcgc cagtttgtca aactcaccac ccaggtcatc
     7741 ttcccagttc ggcccttttt cgattttcgc catccgttcc ccggtaatca gcgaacgcgg
     7801 acgggcaatc ggctgcgatt tgctgccttc cggcgcggta tgcaggttct gatagtcaaa
     7861 atcgaacggc tcgtaataat cgtcgatccc cggcagatgc gggttagcga agattttacc
     7921 cagcaacatg gcacggttac ccatgcgcgg ctgcagtttg ccgttgattt tacggatcca
     7981 gccgcctttg tatttttcct ggttttccca gtcagtcggg aagccctgac ccggcttggt
     8041 ttccacgttg ttgaaccacg cgtattccac gccttcacgg ctggtccaga cgtttttaca
     8101 ggtaactgaa caggtgtggc agccgatgca cttatcgaga ttcagcacca tgccgacttg
     8161 tgaacgaatt ttcattttac gctctcctgt acctggtcat tgccttcgcc atctaaccag
     8221 tcaatgttct tcatcttacg cactacaaca aactcatcgc ggttagaacc tacggtgccg
     8281 tagtagttaa agccgtatgc cagatgggca tagccgccga tcatatgcgt cggtttcggc
     8341 gtgatacggg tgaccgagtt atggatacca ccacgctgtt gggtaatttc cgaaccaggc
     8401 aggttaacga tacgttcctg cgcgtggtac atcatggtca tccctgccgg aacacgctgg
     8461 ctgacaaccg cacgggcagt cagagcaccg ttgctgttga atacttcaat ccagtcgtta
     8521 tcggcgatac ccagatcttt ggcatcggct tcactcaacc agaccaccgg accaccgcga
     8581 cctaaagtca gcatcagcag gttgtcgctg taggtggagt ggatacccca cttctggtgc
     8641 ggcgtcagga agttgagcgc tttttccggg ttgccgttgg atttctggcc tatcacttct
     8701 ttcaccgaac gggtgtcgat cggcggacga taaaccagca ggctttcacc gaaatcacgc
     8761 atccactggt gatcctgata cagttgctga cgaccagaga gcgtacgcca tgggatcagc
     8821 tcgtgaacgt tggtgtaacc ggcgttgtaa gaaacgtgtt catcttccag accagaccag
     8881 gtcgggctgg agataatttt gcgcggctgt gcctgaatat cgaggaagcg gatcttctcg
     8941 tcttctttat tcagcgccag atgcgtatgg tcacgaccgg taaattcgct cagggcagcc
     9001 caggctttca cggctacctg accgttggtt tccggtgcca gtgtcaggat catctctgcc
     9061 gcatcaattg cggtgttcag catcggctgg cctttcgccg gaccttccgc tttggtgtag
     9121 ttgagcttac gcagcagatc catctcgctc tgggtgttcc aggcaatccc tttaccgcca
     9181 ttaccgattt tctccatcaa cgggccaata gaggtaaagc gttcgtaagt cgccggataa
     9241 tcgcgctcta cgaccataat gtgtggcgcg gttttacctg ggatcaggtc gcactcgcct
     9301 tttttccagt ctttcacatc cagcggctgc gccagttcag cggcagagtc atgctggata
     9361 ggcagcgtga cgatgtcggt ttctttaccc agatggccga cgcacacttc ggagaatttc
     9421 ttcgcgatgg ctttgtagat ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc
     9481 gcagacagcg ggtgaataaa cggatgcata tccgaagtat tcatgtcgtc tttttcgtac
     9541 caagtcgccg tcggcaaaat gatgtcggaa tagagacagg tgctcgacag acggaagtcc
     9601 agcgtaacca ccagatccag cttgccttcc agaccattgt cctgccagtc cacttcttcc
     9661 ggcttcacgc cgccctgttg ccccagatct ttaccctgga taccgtgctc cgtccccagc
     9721 aggtacttga gcataaactc atgaccttta ccggaagaac cgagcaggtt agaacgccag
     9781 atgaacaggt tgcgcgggtg gtttttaccg ttttctggtt gttctgccgc aaaacggatg
     9841 gaaccctctt tcagggattt caccgtatag tccaccggat tcatcccggc tttttccgct
     9901 tcgccagcga tagtcagcgg gttagtgcct aactgcggtg cagacggcag ccagcccatg
     9961 cgttccgcac ggacgttaaa gtcgatcaag tgtccggtat agcgggattt gtccgccatc
    10021 ggtgacagca actcttccgc cgtgacggtt tcataacgcc actggctgga gtggttatag
    10081 aaataagaag tgctgttcat gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc
    10141 ggctgccagc cggtttgcgg acgcagtttt tcctgaccta catagtgcgc ccagccgccc
    10201 ccgctctgac cgacacagcc gcagaaaatc agcatgttga tcagaccacg atagttcata
    10261 tcgaggtgat accagtggtt cagccccgca ccgacgataa tcatcgaacg accgtgcgtt
    10321 ttatcagcgt tatcggcaaa ttcacgggcg atgcgaataa tctggctgcg agaaacgccg
    10381 gtaatctgct cggcccaggc cggggtataa gctttcacat cgtcatagct ggttgcacag
    10441 ttaacgtcgt tcaggccacg ttccagaccg tagtttgcca gcgtcagatc ataaacggtg
    10501 ctcaccaggg cggtgctgcc atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc
    10561 agcacgtttt ccagttccac tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg
    10621 aagcctacct cagcgatctc atcctggcta cccagcaggc tcagttgcag ctcggtttct
    10681 tcgccagttt tgccgtcgcg ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag
    10741 ccaatagaac cgttcggcgc aaccatttcg ccattggtat taaaggcgac agttttccat
    10801 tccggattgt tttcctggcc cagcgcatca accagatcag cagcgcgcag catacgacct
    10861 gcagcgtagt aaccgtcgcg ttcttccagc atcaccagca tcggcatgtc ggtgtagcga
    10921 cgcacatagt cggtgaaata ctggcttggg ttgtcgaggt ggaattcacg cagcattacg
    10981 tggcccatcg ccagcgccat tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg
    11041 cacagtttgg cgatttcagc gtagtctggt gtgacggcaa cagttttggt gcctttgtaa
    11101 cgcacttcag taaagaagtg agcatccggg gtacgcgtct gcggcacgtt tgaaccccag
    11161 gcgatgatgt agctggagtt ataccagtca gcagattccg gtacgtcagt ttgctcgccc
    11221 caggtttgcg gagacgcagg aggcaggtcg cagtaccagt cgtagaagct taagcaagta
    11281 ccgccaatca gcgagagata gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga
    11341 attggcgaga aaccagcaac acggtccggg ccgtagtttt tgatggtgta aacgttagat
    11401 gcggcgatca gttcgttcac ctcctgccag gaagaacgaa caaatccacc gcgtccacgc
    11461 gcctgcttaa agcttttcgc tttatcggcg tcttcaatga tagaagccca tgcctcaacc
    11521 ggatcgctat gcagcgcctt cgcttcacgc cacattttca tcaggcgttt gcgcatcatc
    11581 gggtatttca ggcggttggc actgtaaaga taccaggagt agctggcacc gcgcgggcag
    11641 ccgcgaggtt catggtttgg cagatccgga cgggtacgcg gatagtcagt ctgctgggtt
    11701 tcccaggtga ccagaccgtt tttgacgtag attttccagc tgcaggagcc ggtgcagttt
    11761 accccgtggg tagagcggac gattttgtca tgctgccaac gctggcgata tccatcctcc
    11821 cagtcacggt tggtattgag aagctggcca tgcccatcgg caaaggtttc acccttctgt
    11881 ttgaagtagc gaaaccggtc caggaattta ctcatcggtt ttctcctgtg ggagcctgtc
    11941 ggccctctga taaatcgaca ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg
    12001 tgggaattga taacgatcaa gagtgatggg gatgaaaaat aaagtaaatt cctaagatac
    12061 ccctttagga taattaacag ccaccacata acagactgta aattaagacg ttttatacct
    12121 gtagttattt aaactattaa gatttttatg cagatgggta ttaaggagta ttccccatgg
    12181 gtaacatatt aatggctctt aatgaaatcc ggcgaaataa gccatataat atgagtatgt
    12241 tgtaactaaa tgacgcataa aaaaagcgcg acatcatgcc gcgcaaagga taatcaaata
    12301 ttaagattta ttttttagaa tgccgaccat ataccgccca ggtaatcact acgcaggcga
    12361 tatagaaaat caaaaatacc ttcattgcgc cgactggcga acccgttaat gccagcgagc
    12421 taccaaacgc tttcgggata aagaagccac caatagcgcc aatcgcagag atgaaaccca
    12481 gcgccgctgc cgtgtcggtt gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg
    12541 ctttcacgcg atccattgtc agtttacgga agatcactga aatcatctgg aaagtggaac
    12601 cactacccag cccagctgtc aggaacagcg ccaggaagac cgcgaagaac gccatgaagc
    12661 ttccgccctg cccgtcagtc ggtaaggtca ggaacagcag gccgctgaaa atcgccatca
    12721 gaataaagtt caccagcgtg acacgagttc cgcccagacg gtcagataat gcaccacctg
    12781 cagaacgcgc cagcgcacca ataaacggcc cgaagaaagc gtattgcaga atctgaacat
    12841 ccgggaactg cgtttttgac agcatcgcaa agcccgcgga gaagccgata aaggagccga
    12901 aggttgccag atacagcagg ctcataatcc acagatgacc ccgtttgagt accggcaact
    12961 gctccttgat ggaggctttc gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg
    13021 tgaagatggc aaggaacggc acccatatcc aggacgcatt cgccagatac agctcagtcc
    13081 catccggctg tttgacgccc tggctaccaa atactgcgaa aatcgacagt gataccacca
    13141 gcggagcaac caactgcatg acgctgacgc ccatgtttcc cagaccacca ttcagaccca
    13201 gcgcgccacc ctgcttctgt ttcggaaaga agaagctgat gtttgccata ctggatgcga
    13261 agttcgcgcc agcaaagccg catagcagag agatgatgat gaagacgcta taaggcgtgg
    13321 aggtatcctg cacggcaaaa cccagccaga cgcaaggaat aatcagaata ccggtgctga
    13381 acgccgtcca gcgacgacca ccgaagatag gaaccataaa ggagtatgga acacgtaata
    13441 acgcgccaga aaccgaaggc agcgcagtca acataaatag ctgatcggtc gtaaaattaa
    13501 aaccgacttt cggtaagttc accgcaacag cgctgaacaa catccatacg caaaacgcca
    13561 gcagcagaca gggaacggaa atccacaggt tacggctggc aatacgttga ccgcgttgtt
    13621 gccagaacgc aggatcttcc ggtcgccaat ctgtaatgac agctccagta gccctttcgg
    13681 gggcggatga gtgactcata gacacctctg atactcgttt cgatgtctgc caccttagtg
    13741 tctgtagcta aaggcaattt gatgtaaatc aaacgataag ataactttat ctatcattga
    13801 tatttatcat tacccatagt gagtacagtg atttcataaa aattatgaga tttttcacgg
    13861 tgctgtaaaa tccctaccct taccgatgta aagcgactaa ccacacggca aataaggagt
    13921 aactctttcc gggtatgggt atacttcagc caatagccga gaatactgcc attccagaat
    13981 gtatcgtcac attcattaag gttattgctc atttaaagcc tgaaggaaga ggtttgcatg
    14041 cttaaacgtt gtctctctcc gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt
    14101 tctactgcta ttggactggc agggatggcg gtttctggct ggctggtgca aggcgttcag
    14161 ggcagcgccc atgcgatcaa caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg
    14221 gcggcagtgc cattaagtga gaaagacaag cccttaatta aagagatgga acaaacggca
    14281 tttagcgccg agttaactcg agcagcagaa cgagacgaac aactggcgca attacagggt
    14341 ttacaagatt actggcgtaa tgaactgatc cctgcactga tgcgtgcaca aaaccgcgaa
    14401 acggtgtcag cggatgtcag ccagtttgtt gccgggcttg atcagctggt atctggtttt
    14461 gaccgcacca cggaaatgcg cattgagaca gtggtactgg tccatcgggt aatggcggta
    14521 tttatggcac ttttactggt gttcactatt atctggttgc gggcgcgact gctacaaccg
    14581 tggcggcaac tgctggcaat ggcgagtgcc gtcagtcatc gcgattttac ccaacgcgcg
    14641 aacatcagcg ggcgcaacga aatggcgatg cttggaactg cattgaacaa tatgtctgca
    14701 gaactggccg aaagttatgc cgtacttgag cagcgggttc aggagaaaac cgccgggctg
    14761 gagcataaaa atcagatcct ctctttttta tggcaggcta accgccgttt gcattcccgc
    14821 gccccgctgt gtgaacgcct gtcacctgta ctcaacggct tacagaattt aaccctgcta
    14881 cgtgatatcg aattgcgggt gtatgacact gatgatgaag agaatcatca ggagtttacc
    14941 tgccagccag atatgacttg tgatgataaa ggctgccagc tctgcccgcg cggcgtatta
    15001 cccgttggtg atcgcggcac gaccctgaag tggcggctgg ctgactctca tacgcagtac
    15061 ggtattttgc tggcgaccct gccgcagggg cgtcatctta gccatgatca acaacaactg
    15121 ctggataccc tggttgaaca actcaccgcc acgctggcgc tggatcgcca tcaggaacgt
    15181 cagcaacagt tgatcgtgat ggaagagcgt gccaccattg cgcgcgaact gcatgattct
    15241 attgcccaat ctctctcttg catgaagatg caggtgagtt gtttacagat gcagggcgat
    15301 gcgctgccag aaagcagccg cgaactgtta agtcagatcc gtaacgaact gaatgcatcc
    15361 tgggcgcagt tgcgtgaatt gctcaccaca ttccgcttgc agctcaccga gcctggatta
    15421 cgtccggcgc tggaggcgag ttgcgaagag tacagcgcca aatttggctt cccggtgaag
    15481 ctggattatc aattgccgcc tcgcctggtg ccttcgcatc aggcaatcca cttgttgcaa
    15541 attgcccgtg aggcattaag taacgccctc aaacattcgc aagcgagtga agtcgtggtg
    15601 acggtggcgc aaaacgataa tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg
    15661 cctgaaaatg ccatccgcag caatcactac ggcatgataa taatgcgcga ccgtgcgcaa
    15721 agtttacgag gcgattgccg cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc
    15781 acctttattc ccgaaaaaac tttcacagac gtccaaggag atacccatga gtaatcagga
    15841 accggctact atcctgctga ttgacgatca cccgatgctg cgaactggcg taaaacagct
    15901 tatcagtatg gcaccagata tcaccgtggt tggcgaagcg agtaatggcg aacagggtat
    15961 tgaactggcg gaatctcttg atcccgatct gatcctgtta gatctcaata tgcccggcat
    16021 gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt
    16081 attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg
    16141 ttatctgtta aaagatatgg aaccggaaga tctgctgaaa gcattgcatc aggcagctgc
    16201 tggcgaaatg gtattaagcg aagcattaac gcctgttctg gccgccagct tgcgcgctaa
    16261 ccgtgccact actgagcgcg atgttaacca gttaacccca cgcgagcgcg atattctcaa
    16321 gctgattgcc cagggtttgc cgaacaagat gattgcccgc cgcctggata tcaccgaaag
    16381 cacagtaaaa gtgcacgtca agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga
    16441 agcagcggta tgggtgcatc aggagcgcat tttctgatta cggttcccag cgcagttcgt
    16501 cgtttgacaa tgcatcgaac ggttcgacaa gcgttagcgt gatctcattg gaggagacac
    16561 gctgcccctg gttatcttcc accaccaccg acaatcgcca gtgattgctc gccccttccc
    16621 cgttctgcca gtcaggcatg atcagcgtcc agccctccgc gctgttggct tgtgcgcccg
    16681 gcgtcaaact taatatctgc gtatcgccct gccaaatcag ttgccgaata ccgtaacgac
    16741 tgcggatttg taatttcagc ggcactgttt cgccaggttt tagatcccac ggcggtgtcg
    16801 ccagaaacac cgttaacgtt tttcgctgtc ggtactcaag agtcggtaga ttatttcgct
    16861 gcggattatc atagcgacta ccacgtaacg actgactttc ggcaacctcg cccgcagaaa
    16921 gttgtttttt gagcggcaca ccaaagcggt aattaagatt cagtccgagg ttattttgat
    16981 tttcgccact ttcaccctgt ttatgctggg ccgtcacagt gactaatggc acaggggtgt
    17041 aatttaatcc cagactcaac gcgacgggat tgtgataacc cgtaccagag ttaaacaaat
    17101 caacacgctc accaaaatac tgttctaccc tgacactggt attgaggtgt tgatagaacg
    17161 gcatgcgcat ccgggctgtc aggtcatacc cgcgcgccat ccgttgttcc tgcgtggctg
    17221 tctgttcatg ccatgcagca aacggctgat aaaagtttgc cgatagtcgc aaatattcgc
    17281 cccacgcttc ggcaccaaag cccgctcgct gaagattttc gtccagcaag ttgtcataaa
    17341 aagtgttata acccaccagc cagttgccgc gcgcccagcg ttgcccaacg cccacattgc
    17401 tcaccaaccc atcatcctgc tgagtaagac caagctggct ccaggtgaga taacgatcat
    17461 tatcttgtaa cggcacaaac cagcttccac gactgccggt gaaatgtcct tcgttatcca
    17521 ctttgacgtc aacactggca tttccccacg gtgatagcca ggactctacg tgctgattaa
    17581 cctgttgact aagcgcgtcg cggacttttc ccaatgcgaa agcttttgcc tgctcgccag
    17641 tatccagccc gttatcattc atactggttt cgccgaaatc tttcacaatt tcagcaaagt
    17701 gtttttcccc atcatggttt tcgggagcca tgcctaaatc cggcagacca tcattgttat
    17761 tatcaaaggg atttgccgct ttttgctcga aggtagattg tgcgttagct gtaccgcctg
    17821 ccgccagcaa gagtaaataa aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg
    17881 taacttccgg taataatgaa aaaccatagc cttaaagaat aacgattact ggcaactttt
    17941 gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaagg tgaccgattg gtcagacgct
    18001 gcagaagcag taagatagaa gatgagtgtc taattccctg aatacaggag taaataatgc
    18061 aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag cgaatccttg tttaacagct
    18121 tgcggctggc cattgcgtta cgagagcagg agagcaatct ggatctgcgt ctgttcctga
    18181 tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc
    18241 agcaaatgct ggagatcctt accgctcaga atgtaccggt gaaattgtgc aaaacctgta
    18301 ccgacgggcg cgggattagc acacttcctc ttattgatgg ggtggaaatc ggtactcttg
    18361 tggaactggc gcaatggacg ctgtcagccg ataaagtgct cacattttaa tgtttgaggg
    18421 ggcgtctgac gccccactat gagggaagtt tactcaggcg aatcccggcc gtaacacacc
    18481 atccggataa ttctccgcca gcagtttttt taccgatacc agcaaatcat tcagcccatc
    18541 gtcctgcatt cccagtttga taagctcctg ttccagtgaa aacaggtatt gtgcgttggt
    18601 tcccagcgga ccgctcgccg ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga
    18661 ttcatattct ggatgtcgcg ggtccataat aaacacaatg gcgtttactg tgcgtccatc
    18721 atcaagatca agctgacacc aggttggcag ataacagccg gtaatcatct cgcgcttcca
    18781 caacagggtt agttcctgct ccagcgtctc ttctggtagt cgataggcga cgcctgtggt
    18841 gcgtccgccc tctttcagtg caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc
    18901 ggtcaggcgc aggcaaaacg cgcgatgcca tccaaccagt gtaccggtgc ataattcggt
    18961 aaactccagt gccggattcc acatcaacga tccataaccg aagatccaca ccggtccctc
    19021 attaggtcga caagccagcg tcgccgccag cgaagccgcc cgttgttctg ctgaccataa
    19081 gagtgattct tcaatggcac caaatgccgt tttacaatcg gcattcatca agaaatcacg
    19141 cgttatcacc ttacacctcc gccactgcat acttccctgc gacaactttt tggtgtcttc
    19201 cggacatttt taccgctcat tttgaaacca ctaacgggac aatatgcaat ccacggacca
    19261 cttgcaaggc aacaccgcac gaatagcttt aactaagcta ccggttttac gattttttat
    19321 gccatttatc atcatcccct ttggcatatt catgcttcac agcagcccag gccactttat
    19381 gcgcggtttc ttcgcgactg gcgtcatcac gccgatcttc tttatcttta tattgatccc
    19441 atgcgctgtt gaacgcttct ttatagatat cctgggcatg agacggtaga acgtgcttta
    19501 cgctttccgg cagatcgctt ttcgttttat acggcatcgt tgactccttt tacaaagttg
    19561 aacattaagt gtggtcaaca atatctactc ttgccaaaac ataagaattt tcatgtcaga
    19621 ctgttcttaa gaaacgctaa aacaaacatt gtctaaacgt aatacttatt tttatgcccc
    19681 aatgagtaaa aaatcgacag cggggtaaaa attcgtaaaa tgttaccgat atccagacac
    19741 tttctgttat ttttcctacc tattttttac attttcgata aattcatctg caaagggata
    19801 accataatgt caaatgctca agaggcggta aaaacccgcc acaaggagac ttcgcttatt
    19861 ttcccggttc tggcgctggt agtgctgttc ctgtggggaa gcagccagac actaccagtg
    19921 gtcattgcca tcaatcttct tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc
    19981 cgtcatgcgg acgtattagc ccatcgcctg ggagaacctt acggttcgct tattcttagc
    20041 ctttcagtgg ttattcttga agtcagtttg atttcagctt taatggcaac cggcgacgcc
    20101 gcgccaacgc taatgcgtga tacgctctat tcaatcatta tgattgttac cggtgggctg
    20161 gttggctttt cattattgtt gggcggtcgt aagtttgcca cccaatatat gaatctgttt
    20221 ggtatcaagc agtatttaat tgcgctgttc cccctggcga taatcgtact ggtatttcca
    20281 atggctctgc ctgcggcgaa tttttcaacc ggtcaggcgt tactggtagc attaatttct
    20341 gcggcaatgt atggcgtatt tttgctgatc cagaccaaaa cgcatcaaag tttgtttgtc
    20401 tacgagcacg aagatgacag tgatgacgac gacccgcacc acggtaaacc gtctgcccat
    20461 agcagcgtgt ggcatgctat ctggttgatt atccatctga ttgccgttat tgcagtcacc
    20521 aaaatgaatg ccagcccgct ggagacattg ctcgacagca tgaatgcccc tgtcgccttt
    20581 actggcttcc tggtggctct gttgattctg tcgccggaag gtttaggtgc gttaaaagca
    20641 gtgttgaaca accaggtcca gcgcgcgatg aatctgttct ttggttcagt gttagcaacc
    20701 atttcgctta ccgtacctgt cgtcacgcta attgccttta tgacgggtaa cgaattacag
    20761 tttgcacttg gtgcgccaga aatggtggtg atggtggcct ctttagtgct gtgccatatc
    20821 tccttctcca ccggacgtac taacgtgctc aatggcgcag cgcatctggc actgtttgcc
    20881 gcctatttga tgacgatatt tgcctgacag ttatatctcc tccgatgacg gaaatcgggg
    20941 gagataagat aaattcctgc ttattgtatt tcttcatact acctggacag ctcgttaatc
    21001 agaattgcga gcctgtacca caacctgcac gaaaccactg caacagctgt tgaaaagccg
    21061 gaatgtactc tttattgcaa acatttcagg gatgaagtga agatgaattg agacgtagta
    21121 tccacaccaa gccggaatta tccggtgagg cgcaatgttg cgggggcttt atccctggtg
    21181 gcattggttg ctggaaagag aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa
    21241 cgggggctaa tcttgaaacc agacacttca agaatagccg ccttgttgtc attacaacaa
    21301 ggcggctata tgacgctcgc gcagtttgcc atgactttct ggcacgacct ggcagcaccg
    21361 atcctggcgg ggattattac cgcagcgatt gtcagctggt ggcgtaaccg gaagtaaggt
    21421 gaaaacgggc gtcaggctgc cgtgatggca atat
//
LOCUS       DAZOZY010000053        21201 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZOZY010000053 DAZOZY010000000
VERSION     DAZOZY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21201
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.053"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(39..440)
                     /gene="nikR"
                     /locus_tag="ACHURQ_001754"
     CDS             complement(39..440)
                     /gene="nikR"
                     /locus_tag="ACHURQ_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR0823553.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(446..1252)
                     /gene="nikE"
                     /locus_tag="ACHURQ_001755"
     CDS             complement(446..1252)
                     /gene="nikE"
                     /locus_tag="ACHURQ_001755"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR0823554.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1249..2013)
                     /gene="nikD"
                     /locus_tag="ACHURQ_001756"
     CDS             complement(1249..2013)
                     /gene="nikD"
                     /locus_tag="ACHURQ_001756"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR0823555.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(2013..2846)
                     /gene="nikC"
                     /locus_tag="ACHURQ_001757"
     CDS             complement(2013..2846)
                     /gene="nikC"
                     /locus_tag="ACHURQ_001757"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR0823556.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2843..3787)
                     /gene="nikB"
                     /locus_tag="ACHURQ_001758"
     CDS             complement(2843..3787)
                     /gene="nikB"
                     /locus_tag="ACHURQ_001758"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR0823557.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3787..5361)
                     /gene="nikA"
                     /locus_tag="ACHURQ_001759"
     CDS             complement(3787..5361)
                     /gene="nikA"
                     /locus_tag="ACHURQ_001759"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0823558.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5472..6059)
                     /gene="acpT"
                     /locus_tag="ACHURQ_001760"
     CDS             complement(5472..6059)
                     /gene="acpT"
                     /locus_tag="ACHURQ_001760"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR0823559.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6114..7163)
                     /locus_tag="ACHURQ_001761"
     CDS             complement(6114..7163)
                     /locus_tag="ACHURQ_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0823560.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7295..8512
                     /locus_tag="ACHURQ_001762"
     CDS             7295..8512
                     /locus_tag="ACHURQ_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0823561.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8516..9073)
                     /gene="dcrB"
                     /locus_tag="ACHURQ_001763"
     CDS             complement(8516..9073)
                     /gene="dcrB"
                     /locus_tag="ACHURQ_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR0823562.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9146..9811)
                     /locus_tag="ACHURQ_001764"
     CDS             complement(9146..9811)
                     /locus_tag="ACHURQ_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR0823563.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            10032..10277
                     /gene="tusA"
                     /locus_tag="ACHURQ_001765"
     CDS             10032..10277
                     /gene="tusA"
                     /locus_tag="ACHURQ_001765"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR0823564.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10379..12577)
                     /gene="zntA"
                     /locus_tag="ACHURQ_001766"
     CDS             complement(10379..12577)
                     /gene="zntA"
                     /locus_tag="ACHURQ_001766"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR0823565.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12651..13277)
                     /locus_tag="ACHURQ_001767"
     CDS             complement(12651..13277)
                     /locus_tag="ACHURQ_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR0823566.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            13418..13777
                     /locus_tag="ACHURQ_001768"
     CDS             13418..13777
                     /locus_tag="ACHURQ_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR0823567.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13780..14049)
                     /locus_tag="ACHURQ_001769"
     CDS             complement(13780..14049)
                     /locus_tag="ACHURQ_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR0823568.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14039..14635)
                     /gene="rsmD"
                     /locus_tag="ACHURQ_001770"
     CDS             complement(14039..14635)
                     /gene="rsmD"
                     /locus_tag="ACHURQ_001770"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR0823569.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            14785..16281
                     /gene="ftsY"
                     /locus_tag="ACHURQ_001771"
     CDS             14785..16281
                     /gene="ftsY"
                     /locus_tag="ACHURQ_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR0823570.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16284..16952
                     /gene="ftsE"
                     /locus_tag="ACHURQ_001772"
     CDS             16284..16952
                     /gene="ftsE"
                     /locus_tag="ACHURQ_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR0823571.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            16945..18003
                     /gene="ftsX"
                     /locus_tag="ACHURQ_001773"
     CDS             16945..18003
                     /gene="ftsX"
                     /locus_tag="ACHURQ_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR0823572.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18248..19102
                     /gene="rpoH"
                     /locus_tag="ACHURQ_001774"
     CDS             18248..19102
                     /gene="rpoH"
                     /locus_tag="ACHURQ_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR0823573.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19373..20476
                     /gene="livJ"
                     /locus_tag="ACHURQ_001775"
     CDS             19373..20476
                     /gene="livJ"
                     /locus_tag="ACHURQ_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR0823574.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(20675..21058)
                     /gene="panM"
                     /locus_tag="ACHURQ_001776"
     CDS             complement(20675..21058)
                     /gene="panM"
                     /locus_tag="ACHURQ_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR0823575.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5043 a         5598 c         5860 g         4700 t
ORIGIN      
        1 tcagttaata tcttttcttt cctcgtttca gaaataactc aatcttcctt cggcaagcac
       61 tgcaaatgcc catgccgcac gccgcgctgg gcgataacgt catcggcaaa atgctgcacg
      121 tcgcccatat cacctttcag cacggcgatt tccagacagt cgtcgtgatt gatatgcaca
      181 tgcagcgtgg cgacggagag atcgtggtga tggtgctggg tggagacaat gcggctggct
      241 aagtcgcgtt tttcgtgttc atacacatag gagagcaccg cgaaaccttg cgtgccgtgc
      301 tgctgagtgg cctcctgggc cagggcgcta cgcagaatat cgcggatagc ttcggaacgg
      361 ttgttataac cacgacgctg gctcaggctg tccagcgtct ccagtaaatc gtcatcaagc
      421 gtgatggtga ctcgttgcat ttgcgttaaa ccttttctgt ggtgcgacgg cgcacgggga
      481 atgcgggtaa taccgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttat
      541 ctcccaccac ctgggtttcg acgatttgtc cgttgtccat caccatcacc cgctgacaga
      601 agcgttccac caggcgtaag tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt
      661 gtagcttttt cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg
      721 cttcatccag aatcagtagt ttcggttcga ccgccagcgc gcgagccagg cagacgcgct
      781 ggagctggcc gccgcttaac tgcggtgggc gtttgtcgag aacgctgtca tcgagatcga
      841 ccgccttcag catttcgctg gcgcgcgcca gttgttcgga ttttttcagt gatagcaggt
      901 ggcgcatcgg ttcacgcagg atctcgcgca cggttttgcg cggattcacg gcgctgatgg
      961 agtcctgaaa taccatctga atatcgcggc ggaacgcttt acgctgggcg cgattgagtt
     1021 tcgccagcgg ttcgccacgc cagctaatat tcccctgcgc gggcgattct aaacccacca
     1081 gcaaccgcgc gagggtactt ttcccgcagc cgctgcgccc cagcagggcg acggtttcgc
     1141 cgcttttcag ggtcagggaa acgttattca gcaccgcctg atgttgatgt tttccgttaa
     1201 atccaccgtg ggcatagtga tgggaaaggc cggagacgtt aagtaaagtc atgatgccag
     1261 ctccataccg tagagggcga gatgagcgga aaccaggctg cgcgttaccg catgtttggg
     1321 ggcgttaaac agcgtttcta catcgccctg ttcgacaatt ttaccttgtg acatcaccgc
     1381 cacgtcatcc gccagacgcg ccacaacgcc catatcatgg gtgaccagca gcattcccgg
     1441 cgcttgtttt tgcataatgc tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc
     1501 gaggtcggtg gtcggttcat cggcgatgat aaacggtgat tcacacagca ccgccatcgc
     1561 aatcatcatg cgctgcaaca tgccgccgct catctcgaac gggtacagct tcagcacgcg
     1621 cgcggcgttt tccagcccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg
     1681 tttccctaac gccaggcagg tttcccgcgc gtgggtgtgc atggtgtgta gcggattaaa
     1741 ggcgctgcgc gggttctgca tgatggtggc aattttgatg ccgcgcaggg cgcagggata
     1801 aaccgctttg ccatcggcta aaatttcccc cgccgtctgg cgaacgccag cgggcaaaat
     1861 gcccagcgtc gcggcgcagg ttaatgattt cccgctgccg ctaccgccga ctaacgccag
     1921 cacgcgcccg cgttgcaggg ttaacgatac accgtgtacc agcggctgcg cggcctgtag
     1981 cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga
     2041 ggatccagat gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac
     2101 agcgccagcc ccggccagaa catttgcagc ggctgggtcc agatatactg gcgcgcgtcg
     2161 ttaatcatca cgccccattc ggcggtcggc gcggtcacac cgaggccaag gaaagacatc
     2221 cccgcgacgt gcagcatcat atggccgata tccagcgttg ccagcaccag cagcgaaggg
     2281 atcaccgcgc ctgccagatg atcgacaaac acccgcacat ggcccgcacc ggaaagccgt
     2341 gacgccagca caaactcgcg ttggcgtagt gaaatcacca agctgcgcac catgcgtgca
     2401 taccacgccc agtgcgacag ggcgatggcg ataattacgt tggtcagccc ggtgccgagc
     2461 acgccaacca taaagaacga cagaatcgag gtcgggaagg tcataaacat atcggcgacg
     2521 cgcatggtgg cctgatcaac gcgcccgcca atcaacccgg cgctgccgcc aataaccagc
     2581 cctaatgtca gcaccagcag caggcaggcc attaccgaac cgagcgacac gcgggtcgct
     2641 gccatcagcc gcgagaaaat atcgcgacct aagtgatcgg tgcccagcca gtgctgcgca
     2701 tccggcgaaa gcaggcgcga cggcaaatca atcgcctggg ggtcatacgg cagccaccac
     2761 tggctggtga gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac
     2821 cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgcgggt
     2881 ccagcgcggc gttgagcaaa tcgacaatca aattacagac cacaaaaacc accaccatca
     2941 tcagcgtaaa gcactgaatc accggatagt cacggttaaa aatcgccgac accgcatagc
     3001 gcccgacgcc cggccaggca aagatgtttt cgataatcat cgtcccgcca atcagttcgc
     3061 cgatgtgcat ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga atatggcggc
     3121 gttcggtctg tttgtcgttc aggccgcgca gacgcgccca agtgacgtga cgctgaccgg
     3181 cgacgtccag catactggcg cgcagcagtc gcgcgttaat cgccagcgac ataaaggcaa
     3241 tggaaaccgc aggcaaaatg atgtgctgcc agccgccgta acccatcgcg ggtagccatt
     3301 gcagatacac cgaaaacgcc atcaccagta aaaacgccag ccagaagtta ggcatcgaca
     3361 cgccaagaaa cgcgatgaaa cgtacggcga aatccggcag acggtcgcga tggcgcgccg
     3421 cccagatacc gagcggtacg gaagtgagca gaattaatac cagcgccgca cctgcaagtt
     3481 ccagcgtggc gggcaggaag ttcagcatat cgtccagcac cgggcgttgg ctggcgaatg
     3541 agataccaaa gtcaagatgc agcgccttcc acaaccaggt accgtactgg acgtacagcg
     3601 gctgatccag cccgagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt
     3661 tagataaacg cagataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa
     3721 tgatcaccga ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac
     3781 gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc
     3841 gtaggggata ttacccagct ccggttttga taccaccatc atcgagatgt agctgatagg
     3901 cagataaacc gcctcgtcat gcagacgggt caaaatgtcg cgatacagcg cctgacgttg
     3961 cgtttcgtca tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gcttgtcggc
     4021 taatccttgc tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc
     4081 gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg
     4141 ctgacgagca tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg
     4201 gcgcatatca gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa
     4261 cgacagttca atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc ccgcaggcag
     4321 cgtccaaccg gctttttcca gcaactcttt cgctttctgc ggatcgtact ggcgcggttt
     4381 caggccgaga ttagcgtaag gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt
     4441 gccatataac gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa gagcttcacg
     4501 tactgccagc tcgttggtgg gggctttggc ggtattgagc gccagcatca cggtttcgat
     4561 cggctgtgac agttgggtgt ggtaagccgg attctggcta aagcgggcga aggtatcgag
     4621 cggtaataac ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc
     4681 gcgggtagtc gggtccggga tgacgttaaa ggtgatcttt ttaatcgctg gcttttcgcc
     4741 ccagtagttt tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgcaaaat
     4801 ccacggtccg gtgccaatcg gcgctttaat tccgttcatg gtttcatggt ttttaaactg
     4861 cgagggagcg ataaaacgga aaggacgggg cagggccagt tcttgcagga aaggatagta
     4921 ggcgcttttc agggtaattt gcagctctgt tttactgagt gctttaacat caacaatctg
     4981 gtttgccagc tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc
     5041 tgccgccgcc tcggcatcga acggttcacc gttggagaat ttcacgtcat cacgcagggt
     5101 gaaggtccag gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac
     5161 cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg
     5221 gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtagtgat
     5281 ttcatctggt gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt
     5341 gcggcggagt gtggagagca tggtaacccc aatggattaa aatagatggc ggaaataagt
     5401 atgacgattt taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc
     5461 gggtgggcgg atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag
     5521 ggggtggggg tgcagatcgc aaggcttaaa ttttctagct gacaatggct gactgacagc
     5581 gaggagtgat aggtgctatc tacgctgacg atttgccagg cgctgccgcc acgctgttta
     5641 acgatggctt ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc
     5701 acggcgtcca tttcagcgtg ttccccgagg ctgaacacgg cgttcgccag ccagcgccag
     5761 ttggcgcgcg ggcgaatcac ttcgatatcg cagccgactt cgccttcatc gctcaacagc
     5821 agagcgatat cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcgaat
     5881 gcaggtttgc cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc
     5941 aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa
     6001 cccggtggca gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata
     6061 tcagggccaa cgtttaatgg aaaatgaaag tgcgtatcgt atcacttgtc gcctcatccc
     6121 ggtaaccgac ttttcggtct gcctggcccc agtaaaatcg ccagtttgct accgcctttg
     6181 gtggtttcca tccagatttt acacacactg gttaatggca ccgaaagtag catccctacc
     6241 gggccgagca gccatcccca aattaacaat gaaagaaata ccaccatggt ggacatcccc
     6301 aggcgatggc ccatcatccg tggttctaaa atattgccga tgaccatatg gaccactaaa
     6361 aacaatgcgc cgaccagaat acattcgtaa acaccattaa acagcagcac ctgaatcatt
     6421 ggcggtacgg cggaaattac cgcgccgata ttgggcacgt agttgagcaa aaacgccagt
     6481 actgcccaca tcagcgcaaa ctgcaccccc atcagctcca gccccagcca gacgatgaca
     6541 cctgtccata aactgagtag cgtcttcaat gcaagatagt gcgaaacgcc tttaagtgcg
     6601 cggtgtaatc ctgcgatgtg aatctgtggg ttattcagcg caaaacgcat tttgtaaggg
     6661 acgtggcgca cttcaaacag cataaaaact acggtcatca ccagcaaaag cacgctcgcc
     6721 attgccccgg aaagcccggt cattagcgct gtggtgaagg taaccacttt ttccgagtcc
     6781 atccgctgca gcattcgctc cggcgacata tgcaaattaa gaaaaggcaa catctcctgc
     6841 aatttaaaaa gtttgcgcgt cagctcctta ttaaacttcg gcagcataga gataaattcg
     6901 ttaaacgatg ccgccagtac gccgaccagc gcggttagtg cgatcagcat caccaccact
     6961 acaatcgtaa tggcaacggg gcgttgtact ccccgacgaa taaaccaggt aacgagcggg
     7021 ttgaggacga tggcaaaaaa cagcgccagt aacagctgca caatgatatc tgccgctgcg
     7081 tgaatgcccg cgaggatcac taccagcgag gccagcttga gcagaatgtg catgcccgtt
     7141 ttatcgggtt gagggatttc cattggggct tccttgtgac tttttgtatt aagtgtagcg
     7201 ggagtcacgc cagcaatatt ctgattctta ctgctgattt tcacgtcagc acgtggtaaa
     7261 aatgaaacac tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag ccgaacccgc
     7321 gctaaacgga ttgcgcctga atttgcgcat tgtctccatt gtcatgttta acttcgccag
     7381 ctacctcacc atcgggttgc cgctcgctgt attaccgggc tatgtccatg atgtgatggg
     7441 ctttagcgct ttctgggcag gattggttat cagcctgcaa tatttcgcca ccttgctgag
     7501 ccgtcctcat gccggacgtt acgccgattt gctgggcccc aaaaagattg tcgtcttcgg
     7561 tttatgcggc tgctttttga gcggtctggg atatctgacg gcaggattaa ccgccagtct
     7621 gcccgtcatc agcctgttat tactttgcct gggacgcgtg atccttggga ttgggcaaag
     7681 ttttgccgga acgggatcga ccctgtgggg cgttggcgtg gttggctcgc tgcatatcgg
     7741 gcgagtgatt tcgtggaacg gcattgtcaa ttacggggcg atggcgatgg gtgcgccgtt
     7801 aggcgtcgtg ttttatcact ggggcggctt gcaggcgtta gcgttaatca ttatgggcgt
     7861 ggcgctggtg gccattttgt tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa
     7921 accgctgccg tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg cactggcact
     7981 ggcttctgcc ggatttggcg tcatcgccac ctttatcacg ctgttttatg acgctaaagg
     8041 ttgggacggt gcggctttcg cgctgacgct gtttagctgt gcgtttgtcg gtacgcgttt
     8101 gttattccct aacggcatta accgtatcgg cggcttaaac gtggcgatga tttgctttag
     8161 cgttgagata atcggcctgc tactggttgg cgtggcgact atgccgtgga tggcgaaagt
     8221 cggcgtctta ctggcggggg cagggttttc gctggtgttc ccggcattgg gcgtagtggc
     8281 ggtaaaagcg gttccgcagc aaaatcaggg ggcggcgctg gcaacttaca ccgtatttat
     8341 ggatttatcg cttggcgtga ccggaccact ggctgggctg gtgatgagct gggcgggcgt
     8401 accggtgatt tatctggcgg cggcgggact ggtcgcaatc gcgttattac tgacgtggcg
     8461 attaaaaaaa cggcctccgg aacacgtccc tgaggccgcc tcatcatctt aaaacttact
     8521 gaataaccag cgtattaatg atgttttctg cggtggtctg cgctttttgc tgatcgtcag
     8581 ctggcagagt aatttgcatg gtcagcagtt gattacccac gttacccaga ataacggaag
     8641 agtacgccgt ctggcctttc gcggagataa tactgtctaa ctgctgcatt ttgtgacctt
     8701 tcagctcaat ggctttgttg gttaccactt gcagctgcgg atcgcggcta cgttgctgat
     8761 cttccagacg cttcgccaac accgccagat cttctttcgg atcatcgccc atgatgacga
     8821 tgactgcttt ctgcccggtg gcgtcggacc agacatgcat gttattggcc tgcgttccca
     8881 gcttaccgct ctggtcggtc atatccgctg gcagcgagaa acttaacttg ccatcaagca
     8941 ggttgacggg attcccggta gcgttacttt ccgcgaccga accctgcgcc gtagcgttag
     9001 tgtctttatc atcacaggcc gcaagcccca taaccagcag gccaattccg acatatttaa
     9061 ccagattgcg cattgacttc ttcctttcga taaacggcca taacggctca ttcatccatc
     9121 ttatcacaac tctgataacg aacctttaac tcgcctgcaa agcgttgatt tcggatttat
     9181 ctgccagtct tttcaacaga atattgagta atacgccata cattggcagg aagaaaacga
     9241 tactgattaa cactttgaaa cagtaatcga ccagcgcgat ttccatccag tgttcagcca
     9301 taaaggcatc cgggctacgc cagaaggcaa tgaagaaaaa ggccagcgtg tcgctgacgt
     9361 taccgaacag tgtggacgct gtcggtgcca gccaccagcg gcgactctga cgcaggcggt
     9421 taaaaacgtg cacgtcgagg atctgtccca gcgcgtaggc catgaaactg gcggtggcga
     9481 tacgggcgac aaacaggttg aagtgggcga gtgcgccgaa tccctgccag gaacccatat
     9541 agaatagcga cgagatgacg taggagatca gcaacgccgg gatcatcacc gcaaagatga
     9601 tacgacgggc cagcggtgcg ccaaaaatac gcacggtcag gtcggtagca aggaaaataa
     9661 acgggaagct aaacgcgccc caggtggtat ggaaacccaa aatggagacg ggaagctgaa
     9721 ccagatagtt actggaggtg atcaccagca gatgaaataa cgataaccag aacaacgcct
     9781 tatagcgttg agtttgcgag aaaacgttca tattgtacct ttttgattaa ccattggggt
     9841 gagggaaccc aatacgtacg acacgtctgt taccttaaca taataacgtg cttacccttt
     9901 tttcgagccg ccgcatgata ctgctttacg ttgacaatgc aatggttaat tttcacgcaa
     9961 tcgttaacct ggtttgctta cggactcaca gggcgtaaac tagcgccgtt tttttatgtg
    10021 atgagaagaa aatgaccgat ctcttttcca gccctgacca cacactcgac gcacttggcc
    10081 tgcgctgccc ggaacccgta atgatggtgc gcaaaactgt gcgcaatatg cagcctggcg
    10141 aaacgttgct gattatcgcc gacgatccgg ccactacccg cgatattcct gggttttgta
    10201 cctttatgga acacgaactg gttgcgaaag agacggatgg actgccttat cgttatttga
    10261 ttcgtaaagg cggttgatgg ggaaagattg gcttcgatgc cgccttttcc cctcacccta
    10321 accctctccc cagaggggcg aggggaccga tcgcgctcaa tgttgcgatc ggtttgcctt
    10381 atctcctgcg caacaatctt aacgcattcg ctgtcaccag caccgtcgca cccgtatctg
    10441 ccagcaccgc cagccacagg ccggtcatcc ctaacagcgt ggtgacgagg aagatccctt
    10501 tcagccccag cgcaatggtg atgttctggc ggatattggc gtgagtggcg cgtgccagtt
    10561 caatcatttg caccaggccg cgcaggtggt tatgggttaa tgctgcatca gcggtttcca
    10621 gcgccacgtc tgtgccactg cccattgcaa tcccaatggc ggcagctttc atcgctggcg
    10681 cgtcgttaat accatcaccg accatcgcca ggggcgcatg ttgattcagc ttggtcaccg
    10741 ctttgacttt atcttccggc aataggcccg ctttaaactc cagccccagc tccccggcga
    10801 ttgccgccgc tgcacgtgga ttatcgccgg tgaggatcac tcctttgacc cccagcgcgt
    10861 tcagttcact gatggcagtt gcagcatcgg cgcgcagggt atcctgcaat gcaatgatac
    10921 ccagcacgtc atcattacgt actaccagca caaccgtttg cccggcgctt tccagttcat
    10981 taatcaaacc agcaaatgca tcagcgggat gtttcccggc agcgcaaatc aatacgcgct
    11041 caccgttaac ctgcgcttca atgccagacc cgaccagcgc ccgctgtgat tcggcggtgg
    11101 gaatggcgag ttcagcaacc tgtgcttcgc gtacgatggc ttgcgccagt ggatgcgtcg
    11161 cgccttgctc gaccgccgcc gccagtgtca gcagttcaga ttcactaata cccgttgccg
    11221 gatgaatcgc ggtaacgcgc ggtttaccga cggtcagcgt accggtttta tcaaacgcca
    11281 cctgagtaac acgacctagt tgttccagcg ccgctccgcc tttaatcaac gccccacgac
    11341 gcgctgccgc cgccagcccg gaggtaatcg ccgcaggcgt tgagataact aacgcacacg
    11401 ggcagccaat cagcagcagc gtcagccctt tataaatcca ctcctgccag ctggcggcaa
    11461 acagcagcgg tggcaccagc gtcaccagca gagcgacggc cataatcgcg ggcgtataga
    11521 tacggctgaa acggtcgata aaccgctcaa tgggggcgcg acgctcttcg gcttcttcga
    11581 tcagtttcag aatccggtca atggcgctgg cacccggttc tgacagcact tccagcgtta
    11641 ccagacggtc tacgctggtg gctcctgcag gaactttatc gcccgtcgcg cgctccaccg
    11701 gaatggattc tccggtcagg gcgctttcat caaaactggc aaacggtgag agcagtttac
    11761 cgtcggcagg caaacgccca cctgcggcga cttcaatcac atcgccaggc cgcaggctgt
    11821 taatcgccac ctcttcccgc tcaccgttac gcaggcgcgt ggcggtttct ggtttcagcg
    11881 ccattaacgc gctaacgccc tgacgcgcgc ggctggcggc ccagccttcc agtcgttcac
    11941 caatcaaaaa cagcagcaac accatcgcag cttcagccgt tgcgccaata aacagtgcac
    12001 caatagcggc tacgctcatt aaggtttcaa tggcgaagta gctgccggat ttgatcagcc
    12061 gtaatgcctg acgagcaatc gggtacagcc caaccagcgt ggtcgcgata aacgccagtt
    12121 gcccgaacgg atgattgaac tgctccagac cccagctgat tgccatcatc acgattagtg
    12181 taatcagcgg cagattctct ttcaggcgcg atgcttgtgg ctcgtcggcg gcctgctcat
    12241 cgcgcaggga atagcccgct ttttgcaccg cagattcaac ttgtgcacga atgtcattgt
    12301 cggcatcaac caccagtttt tcggtggcga acaacacctg cacctgattc acacctgcaa
    12361 gctggcgcac ggcattttct accttgcgcg cacaggcggc gcagtccatg ccactgactt
    12421 tccagctata gcgggtgccg gagacgtttt cagagagcgt tggcgagctg gagcatgcgc
    12481 cgtcgcagca acagtcgttg gcgttctgta ccgtggttag cggtttgaac gcagcaaatt
    12541 gaggggcttt cttgccgtga ttgtcaggtg tcgacatggc atactccggt taagtttttt
    12601 ctcattaacc gaaggataca ctctggagtc gactccagag tcaagtttta tcagagatac
    12661 agtgagcgga tgatgaggaa gtgaccggca aagtagcagg ccgcagcaat cgcgttatcc
    12721 gcacggaagc gtcggcgata gtggctcccc agccagacaa agttactgat aaacagcaac
    12781 gaagcgccaa caaacgcaga gagcgccgga gcggtcggac ggaagaacca cagttcacct
    12841 gccagccaca ccatcaccag cgtcatgccg ataaacgtgc agataggcca acgatactct
    12901 tccagccgcg tccagataat cgccagtaac agcgcaccca gaaccagcaa caccagtggc
    12961 agaggccaga agaaagagag cgtcatctga ctggcgaaat agatggtgta cagcaggtgc
    13021 gagaggaaaa acgcgccgat ggcgtacatc agacgttgac gtggcaacag ggttagcgca
    13081 tcgcccagca gtgaggcgca cagccctgcc agcaccagat agctaatggc gtcgaacatc
    13141 ggcgcttgcc aggccagtaa cagcaggaga agaagggtta acggtttaaa cacccagcgt
    13201 tgccaggttg gcccacgata cgatgcatcc acagatagcc atgcggaaag acagacagcg
    13261 ataaacgacc aaagcatctt agttccttga attcgttatt ttttcgtaag cggaatgcct
    13321 acggtcttgc ttccagttta gtggcgaggg tggacggatg acaacaccat aactggcagg
    13381 gtatgcttat ttccgcattt tccgatgagg gataaagatg agcaaaccac ctcttttctt
    13441 tattgttatc attggcttaa ttgtcgtcgc cgcatcgttt cgttttatgc aacagcgacg
    13501 ggaaaaagct gataatgata tggctccgct ccagcaaaag ctggtggtgg tgagcaacaa
    13561 gcgggaaaaa ccgattaacg atcgccgttc gcgccagcag gaagtgactc cggcaggcac
    13621 cagtatgcgc tacgaggcaa gcttcaaacc gcaaagcgga ggaatggagc agacgtttcg
    13681 cctcgacgcc cagcagtacc acgccctgac agtgggcgat aaaggtacgc tgagctataa
    13741 aggaacgcgc tttgttagct ttgttggcga acaataaggt tattttttaa ctttaaattt
    13801 cttctgccag accagcagtt caaacacgcc gaaaaggaaa atccgtacct tttcggcggt
    13861 ggtcatctgc gggccatctt tcggtaaagt ggatttcaat agcgccagct gcataccatg
    13921 catcagcacg gtaaaaatca gcgccacgtt aacgaaaata tttagcgggc gcgggaaggg
    13981 atgcaccaga ttgaggatta aaaatcccca aacgcagagc attaacaaac gaccaatatt
    14041 aatcagcatc actttctcct tgtgcttcgc gttgatacag ccgataagcc acctgacccg
    14101 ccactttttc ccgatgtaac gaccagtttg ccggaacggt gggcagaccg ttttcgactt
    14161 cgctttcgac ataaatcagg gcttcgtcag ccagccagcc gttatcttcc agtaaattta
    14221 tcgtctcttc taacaagcca cggcggaacg gtggatcgac aaacacgata ttatgcggtg
    14281 tgcctttttg cgccaggaat gacatcgcgt tgctgttcac cacgcgtgca ttgcctgctt
    14341 ttagtgtcgc cagattctta attaactgct gagaaactgc acgatccatc tcaattaacg
    14401 tggcccccgc agcgtagcgc gataacgctt ccagccccag cgcgccgctc ccggcgaagc
    14461 aatccagaca ttgggcgtca acaatgaccg gagccagcca gttaaacaac gtttcgcgta
    14521 cgcggtcggt ggtggggcgc agacctgggc tatcaggaac cgggagttta cggcctcgcc
    14581 actgcccgcc aataatgcgg atttggccgc tgccggaatg attcggtttt ttcatgataa
    14641 attgctcaat ccgccagata acacatactt gcaggcggtg atgtgaatta agttaagtga
    14701 tagactattt catcattttt ttagctgcta tgtacatagc gttaacgctg tgccatgaag
    14761 caacagcgag gagtgtagtc gcaaatggcg aaagaaaaaa aacgtggctt tttttcctgg
    14821 ctgggctttg gtcaaaaaga gcagaccccg gaaaaagaga cagaagttca gaatgaacaa
    14881 ccggttgtag aagaaatcgt tcaggcgcaa gagcctgtga aggcttcgga acaagccgtt
    14941 gaagagcagc cgcaggcgca tactgaagcc gaggcggaaa cttttgctgc cgacgttgtg
    15001 gaagtcactg aacaggttgt tgaaagtgaa aaagcgcagc ctgaagcgga agttgttgca
    15061 cagccggaac cggtcgtaga agaaacgccg gagccagtgg ctatcgaacg tgaagagctg
    15121 ccgttgccgg aagacgtcaa cgccgaagag gtttcgccag aagagtggca ggctgaagcg
    15181 gaaaccgtag agattgtcaa agcggcggaa gaagaagcgg ctaaagaaga aatcaccgac
    15241 gaagagctgg aagcacaggc gctggcagcc gaagcagcag aagaggcggt gatggtggtt
    15301 cctccggcag aagaagagca gccggtggaa gaaatcgctc aggagcagga aaaaccaacc
    15361 aaagaaggtt tcttcgcgcg cctgaaacgc agcctgttaa aaaccaaaga aaatctcggt
    15421 tccggattta tcagcctgtt ccgcggtaaa aaaatcgacg atgatctgtt tgaggagctg
    15481 gaagagcagc ttttgatcgc cgatgtgggc gtggaaacca cacgtaaaat tatcaccaat
    15541 ctgacggaag gcgcatcccg caagcagctt cgtgatgccg aggcgctcta tggcctgctg
    15601 aaagaagaga tgggtgagat tctggcgaaa gtcgatgagc cgctgaatgt tgaaggtaaa
    15661 acgccgttcg tgatcctgat ggtgggcgtc aacggtgtgg gtaaaaccac gacgattggt
    15721 aagctggcac gtcagtttga gcagcagggt aaatcggtga tgctggcggc gggcgatact
    15781 ttccgtgcag cagcggttga acagcttcag gtctggggtc agcgcaacaa tattccggtg
    15841 attgcccagc ataccggggc ggattccgcc tctgttatct tcgacgccat tcaggcagct
    15901 aaagcgcgta atatcgacgt cctgattgcc gataccgccg gacgcctgca gaacaaatcg
    15961 cacctgatgg aagagttgaa gaaaatcgtc cgcgtgatga agaaactcga cgttgaagcg
    16021 ccgcatgaag ttatgctgac tattgatgcc agcaccgggc agaacgcggt aagccaggcc
    16081 aaactgttcc acgaagctgt tggcttaacc ggcatcacgc taacgaaact ggacggcacg
    16141 gcgaaaggcg gggtaatttt ctcggtggct gaccagtttg gtatccctat ccgctacatt
    16201 ggtgtcggcg aacgtattga ggatttgcgt ccgtttaagg cggacgactt tatagaggca
    16261 ctttttgccc gagaggatta acaatgattc gctttgaaca tgtcagcaag gcttatctcg
    16321 gtgggagaca ggcgctgcag ggcgttacgt tccatatgca gccgggtgag atggcgtttc
    16381 tgaccggtca ttccggcgca gggaaaagta ccctcctgaa gctgatctgt gggattgagc
    16441 ggcccagcgc cgggaaaatc tggtttagcg gccatgacat cacgcgtctg aaaaaccgtg
    16501 aagttccgtt tctgcgccgc cagattggca tgattttcca ggatcaccat ctactgatgg
    16561 accgtactgt ctacgataac gtggcgatcc cgctgattat cgctggtgcc agcggtgacg
    16621 atattcgtcg ccgggtgtcg gcggcactgg ataaagtcgg gctactggac aaagcgaaga
    16681 acttccctat tcagctttcc ggcggtgaac aacagcgtgt tggcattgcc cgcgcggtgg
    16741 tgaataagcc cgcggtactg ctggcggacg aaccgaccgg taacctggac gacgcgttgt
    16801 cggaagggat tctacgtctg tttgaagagt ttaaccgcgt tggggtaacc gtattgatgg
    16861 caacgcacga catcaacctg atttcacggc gttcccatcg catgctcacc ctgagcgatg
    16921 gtcacttgca tggaggcgtg ggccatgaat aagcgcgatg caatcaatca tattcggcag
    16981 tttggtgggc gtctggatcg cttccgtaaa tcggtcggcg gctcaggcga cggcggtcgt
    17041 aacgcaccga aacgcgcgaa atcctcgcca aaaccggtaa atcgcaaaac caacgttttc
    17101 aacgaacagg tgcgctatgc cttccacggc gcattgcagg atctgaaaag caaaccgttc
    17161 gccacgtttt taacggtgat ggttatcgcc atctccctga cgctgcccag cgtctgttat
    17221 atggtgtaca aaaacgttaa tcaggcggcg acgcagtatt atccgtcacc gcaaatcact
    17281 gtttacttgc aaaaaacgct ggacgatgac gccgctgcgg gcgtggtggc acagttgcag
    17341 gccgagcaag gcgtggagaa agtgaactat ctttctcgtg aagacgcact gggcgagttc
    17401 cgtaactggt ctggttttgg tggtgcgctg gatatgctgg aagaaaaccc gcttccggca
    17461 gtggcggtgg tgatcccgaa actcgatttt caggggacgg aatcactcaa tacgctgcgt
    17521 gatcgtatca cgcagattaa cggcattgac gaagtgcgga tggatgacag ctggtttgcc
    17581 cgtctggcgg cgttgaccgg gctggtcggg cgcgtttcgg cgatgatcgg cgtgttgatg
    17641 gtggcggcag tgttcctcgt catcggcaac agcgtacgtc tgagcatctt tgctcgccgt
    17701 gactccatca acgtgcagaa actgattggt gcgacggatg gtttcatcct tcgcccgttc
    17761 ctgtatggtg gcgcactgct gggattttct ggcgcattgt tgtcattaat tttgtcagaa
    17821 attctggtgc tgcgattgtc atcggcggtt gcggaagtgg cacaggtttt cggaacgaag
    17881 tttgatatca atggtttatc gttcgatgaa tgcctgctat tgctgctggt atgctcgatg
    17941 attggctggg tggcagcgtg gcttgccacg gtacaacatt tacgccactt tacgcctgaa
    18001 taataaaagc gtgttatact ctttccctgc aatgggttcc gtagcaggga aagagatccc
    18061 gttgtctctt cccggtattt catctctatg tcacattttg tgcgtaattt attcacaagc
    18121 ttgcattgaa cttgtggata aaatcactgt ctgataaaac agtggatgat aacctcgttg
    18181 ctcttaagct ctggcacagt tgttgctacc actgaagcgc cagaagatat cgattgagag
    18241 gatttgaatg actgacaaaa tgcaaagttt agctttagcc ccagttggca acctggattc
    18301 ctacatccgg gcagctaacg cgtggccgat gttgtcggct gacgaggagc gggcgctggc
    18361 tgaaaagctg cattaccatg gcgatctgga agcagctaaa acgctgatcc tgtctcacct
    18421 gcggtttgtt gttcatattg ctcgtaatta tgcgggctat ggcctgccac aggcggattt
    18481 gattcaggaa ggtaacatcg gcctgatgaa agcagtgcgc cgtttcaacc cggaagtggg
    18541 tgtgcgcctg gtctccttcg ccgttcactg gatcaaagca gagatccacg aatacgttct
    18601 gcgtaactgg cgtatcgtca aagttgcgac caccaaagcg cagcgcaaac tgttcttcaa
    18661 cctgcgtaaa accaagcagc gtctgggctg gtttaaccag gatgaagtcg aaatggtggc
    18721 ccgtgaactg ggcgtaacca gcaaagacgt tcgtgagatg gaatcacgta tggcggcaca
    18781 ggacatgacc tttgacctgt cttccgacga cgattccgac agccagccga tggcaccggt
    18841 gctctatctg caggataaat catctaactt tgccgacggc atcgaagatg ataactggga
    18901 agagcaggcg gcaaaccgtc tgaccgacgc gatgcaaggt ctggacgaac gcagccagga
    18961 catcatccgc gcgcgctggc tggacgaaga caacaagtcc acgttgcagg aactggctga
    19021 ccgttacggt gtttccgctg aacgtgtacg ccagctggaa aagaacgcga tgaaaaaact
    19081 gcgcgctgct atagaagcgt aatttccgct attaagcaga gaaccctgga tgcgagtccg
    19141 gggtttttgt tttttgggcc tctacaataa tcaatttccc ctccggcaaa acgccaatcc
    19201 ccacgcagat tgttaataaa ctgtcaaaat agctattcca atatcataaa aatcgggtat
    19261 gttttagcag agtatgctgc taaagcacgg gtagccatgc ataaaacgaa ataaagtgct
    19321 gaacaacaac atcacaacac acgtaataac cagaagaatg gggattctca ggatgaacat
    19381 aaagggtaaa gcgttactgg caggatgtat cgcgctggca ttcagcaata tggctctggc
    19441 agaagatatt aaagtcgcgg tcgtgggcgc aatgtccggt ccggttgcgc agtacggtga
    19501 ccaagagttt accggcgcag agcaggcggt tgcggatatc aacgctaaag gcggcattaa
    19561 aggcaacaaa ctgcaaatcg taaaatatga cgatgcctgt gacccgaaac aggcggttgc
    19621 ggtggcgaac aaagtcgtta acgacggcat taaatatgtg attggtcacc tctgttcttc
    19681 atcaacgcag cctgcgtctg acatctacga agacgaaggc attttaatga tcaccccagc
    19741 ggcaaccgcg ccggagctga ccgcccgtgg ctatcagctg atcctgcgca ccaccggcct
    19801 ggactccgac caggggccga cggcggcgaa atatattctt gagaaagtga aaccgcagcg
    19861 tattgctatc gttcacgaca aacagcaata cggcgaaggt ctggcacgag cggtgcagga
    19921 cggcctgaag aaaggcaatg ctaacgtggt gttctttgat ggtatcaccg ccggggaaaa
    19981 agatttctct acgctggtgg cgcgtctgaa gaaagagaat atcgacttcg tttactacgg
    20041 cggttaccac ccggaaatgg ggcaaatcct gcgtcaggca cgcgcggcag ggctgaaaac
    20101 tcagtttatg gggccggaag gtgtggctaa cgtttcgctg tctaacattg cgggcgaatc
    20161 agcggaaggg ctgctggtga ccaagccgaa gaactacgat caggttccgg caaacaaacc
    20221 cattgttgac gcgatcaaag cgaaaaaaca ggacccaagc ggcgcattcg tttggaccac
    20281 ctacgccgcg ctgcaatctt tgcaggcggg cctgaatcag tctgacgatc cggctgaaat
    20341 cgccaaatac ctgaaagcga actccgtgga taccgtaatg ggaccgctga cgtgggatga
    20401 gaaaggcgat ctgaaaggct ttgagttcgg cgtatttgac tggcacgcca acggcacggc
    20461 gacagatgcg aagtaatcat taatcggcaa ctttgggttg ccgccaaatt gctaatatcg
    20521 agtacgttgc ttcatgccgg atgcggcgta aacgccttat ccggcctata agatccaaag
    20581 aaatcagtaa attgcaacac acattgtagg cctgataagc gtagcgcatc aggcaatttc
    20641 tcgttggtca tcacttttga aatcaacttg acgattaacg cttctcccag ccgccctgtt
    20701 gcgccgtaaa tcccagtgcc tgcataaacg ctgtcatcac accgcgatct tccacgcctg
    20761 catccgctat ccaccagcat gaaacgccag gattgttacg caaaacctct tccagcagat
    20821 attgccccac accgcgacgt cgggtgactt cccgcacacg cagggaatcc agtgctccct
    20881 cggtgccgct taaggttacc cgtacggcag cgagcaggcg ctcgttaaaa cgcgcggcgt
    20941 agatacggtg gttatcgtca acctgtaaag aggaagggga atactccggc cagatctttt
    21001 gcaggtcaat ccggtcctgg tcgctaaagt tttctaatcg aatgatggtc agcttcatgg
    21061 gtaacccgtg taaatcacaa aagtggaacc agtgtagcga aataatttaa tcggaggctt
    21121 tctctttttt atttcttttg gcaggtgatt aattttttaa cagcaataat tacaaagtta
    21181 aaacattaga gaatgaaaaa t
//
LOCUS       DAZOZY010000054        20944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZOZY010000054 DAZOZY010000000
VERSION     DAZOZY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.054"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            129..671
                     /locus_tag="ACHURQ_001777"
     CDS             129..671
                     /locus_tag="ACHURQ_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR0823576.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            771..1973
                     /locus_tag="ACHURQ_001778"
     CDS             771..1973
                     /locus_tag="ACHURQ_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR0823577.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2193..2975
                     /locus_tag="ACHURQ_001779"
     CDS             2193..2975
                     /locus_tag="ACHURQ_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR0823578.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2990..4195
                     /gene="rhmD"
                     /locus_tag="ACHURQ_001780"
     CDS             2990..4195
                     /gene="rhmD"
                     /locus_tag="ACHURQ_001780"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR0823579.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4252..5541
                     /locus_tag="ACHURQ_001781"
     CDS             4252..5541
                     /locus_tag="ACHURQ_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0823580.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5559..6362
                     /gene="yfaU"
                     /locus_tag="ACHURQ_001782"
     CDS             5559..6362
                     /gene="yfaU"
                     /locus_tag="ACHURQ_001782"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR0823581.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(6403..7305)
                     /locus_tag="ACHURQ_001783"
     CDS             complement(6403..7305)
                     /locus_tag="ACHURQ_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0823582.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(7498..8688)
                     /gene="glpC"
                     /locus_tag="ACHURQ_001784"
     CDS             complement(7498..8688)
                     /gene="glpC"
                     /locus_tag="ACHURQ_001784"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR0823583.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8685..9944)
                     /gene="glpB"
                     /locus_tag="ACHURQ_001785"
     CDS             complement(8685..9944)
                     /gene="glpB"
                     /locus_tag="ACHURQ_001785"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR0823584.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(9934..11562)
                     /gene="glpA"
                     /locus_tag="ACHURQ_001786"
     CDS             complement(9934..11562)
                     /gene="glpA"
                     /locus_tag="ACHURQ_001786"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR0823585.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            11835..13193
                     /gene="glpT"
                     /locus_tag="ACHURQ_001787"
     CDS             11835..13193
                     /gene="glpT"
                     /locus_tag="ACHURQ_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR0823586.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13198..14274
                     /gene="glpQ"
                     /locus_tag="ACHURQ_001788"
     CDS             13198..14274
                     /gene="glpQ"
                     /locus_tag="ACHURQ_001788"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0823587.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14316..14558)
                     /locus_tag="ACHURQ_001789"
                     /pseudo
     CDS             complement(14316..14558)
                     /locus_tag="ACHURQ_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14553..14657
                     /locus_tag="ACHURQ_001790"
                     /pseudo
     CDS             14553..14657
                     /locus_tag="ACHURQ_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14737..15386
                     /gene="inaA"
                     /locus_tag="ACHURQ_001791"
                     /pseudo
     CDS             14737..15386
                     /gene="inaA"
                     /locus_tag="ACHURQ_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(15440..15694)
                     /gene="yfaE"
                     /locus_tag="ACHURQ_001792"
     CDS             complement(15440..15694)
                     /gene="yfaE"
                     /locus_tag="ACHURQ_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR0823588.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15694..16824)
                     /gene="nrdB"
                     /locus_tag="ACHURQ_001793"
     CDS             complement(15694..16824)
                     /gene="nrdB"
                     /locus_tag="ACHURQ_001793"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0823589.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17058..19343)
                     /gene="nrdA"
                     /locus_tag="ACHURQ_001794"
     CDS             complement(17058..19343)
                     /gene="nrdA"
                     /locus_tag="ACHURQ_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0823590.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            19471..19614
                     /gene="ypaB"
                     /locus_tag="ACHURQ_001795"
     CDS             19471..19614
                     /gene="ypaB"
                     /locus_tag="ACHURQ_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR0823591.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            20039..>20944
                     /locus_tag="ACHURQ_001796"
     CDS             20039..>20944
                     /locus_tag="ACHURQ_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR0823592.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT         4922 a         5080 c         5652 g         5290 t
ORIGIN      
        1 ttatttaaga agtatttagt tacaataagt gactagatcc cttttttcgc tcattgaatc
       61 ctctgtgcca ggcagtacaa tcccgcagct ttgggaattt tgattaattg cttgtgggaa
      121 aggggataat gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg gtttctgcat
      181 cggtcaatgc aatgagcatc agcggtcagg cgggtaaaga atacaccaat attggtgtcg
      241 gttttgggac tgaatcgacg ggcctggcgt taagcggtaa ctggacacat aacgacgacg
      301 acggtgacgt cgctggcgtg gggctggggt tgaatctgcc tctcgggccg ttaatggcga
      361 ccgttggcgg aaaaggcgtt tacaccaacc cgaattacgg cgatgaaggt tatgccgcag
      421 cggtaggagg tggtttgcag tggaaaattg gcaacagctt ccgtttgttt ggcgagtatt
      481 actactctcc ggattcactc tccagcggta ttaaaagtta tgaggaagcg aatgctggcg
      541 cgcgttacac cattatgcat ccagtcagta ttgaggcggg ttatcgctac ctgaatctgt
      601 cgggtaaaga cggtaaccgc gacaacgccg tggctgacgg cccgtatgtt ggggttaacg
      661 ccagtttctg atcccttccg gcgcggcatt ctgtcgcgcc attcagtttc ttactacgcc
      721 ttatctccct ttgcgttata gtgattcgat aacaaaagca ggagataacc atgttaaaag
      781 tggaaatgtt atccaccggg gatgaagtgt tacacgggca aatcgttgac actaacgctg
      841 cctggctggc cgattttttc tttcatcagg ggttgccatt atctcgccgc aatacggtgg
      901 gggataacct tgatgactta gtcaccattc ttcgcgaacg tagtcagcac gccgatgtgc
      961 tgatcgttaa cggcgggctg ggaccgacca gtgatgattt aagtgcactc gccgctgcga
     1021 cagcaaaagg tgaaggcctg gtgctgcatg aagcctggct caaagagatg gaacgctatt
     1081 tccacgaacg tggacgagta atggcaccga gcaaccgtaa acaagcggag ctgcctgcca
     1141 gtgctgaatt tatcaataac ccggtaggca ccgcctgtgg ttttgccgtg cggcttaatc
     1201 gttgcctgat gttctttact cccggcgtac cgtcagaatt taaggtgatg gtcgagcacg
     1261 aaatcctgcc gcgcctgcgc gagcgttttt ctttaccaca gtcgccggtt tgtctgcgtt
     1321 taactacctt tggtcgttcg gaaagcgatc tggcacaaag cctggacact ctacaactgc
     1381 cgccgggtgt aacaatgggc tatcgctcct caatgccgat catcgaactg aaactcaccg
     1441 gaccggcaag cgagcaacag gcgatggaaa aactgtggct ggacgttaaa cgtgttgccg
     1501 gacagagcgt gattttcgaa ggcaccgaag gactgcccgc gcagatcagt cgcgaattgc
     1561 aaaaccgcca gttcagcctg acgttgagcg agcaattcac cggtggttta ttggctttgc
     1621 aactttctcg cgcaggtgct ccattgctgg cgtgtgaagt ggttccttca caggaagaaa
     1681 ccctggcgca aactgcgcac tggattacag aacggcgggc caaccatttt gccgggctgg
     1741 cactggctgt ttcgggtttc gagaacgagc atctcaactt tgcgctagcc acgccagacg
     1801 gcactttcgc tctgcgtgtg cgtttcagca ctacacgcta cagcctggct atccgtcagg
     1861 aagtgtgcgc aatgatggca ctgaatatgc tgcgccgttg gttaaacggc caggatatcg
     1921 ccagtgagca tggctggatt gaggttgttg agtccatgac cttatctgtc tgattccgcc
     1981 actctgtgcc ggtttactgc accggcacat tccccctccg gcaaaacttg tccaacctgt
     2041 tgtactcaat aagaaaaaat ccgtgcactg attcacaaat ttgtgcgtaa tttctccagt
     2101 ccgctggacg aggagcggga tttgatgcgc ttcacaattt tactccccct gagcaacgag
     2161 actgattcca gtatctaaga ctggagtcca gtatgttgga atcaagcaaa gttcccgccc
     2221 tgacacgggc tattgatatt ctcaatttga ttgcccgcat tggcccctgt agtgctgcga
     2281 cgatcattga cacattggga atccctaaaa gcacggccta tttgctgctt aatgaactca
     2341 ggcgtcagcg ttttcttagc cttgatcacc aggaaaattt ttgtttgtgg accaggctgg
     2401 tggagctttc tggtcatgcg ttaagcaaga tggatctccg ggaactggct cgaccgcgtt
     2461 tgacgcaact gatggatacc accgggctgt tatgtcacct ggggattatt gataacggca
     2521 gcgcttatta cattttaaaa gtggagtcat cagccactat cagcgtgcgt tcccatgaag
     2581 gaaaaagcct ttcgctttat cgctccggaa taggtaaatg cctgcttgcc tggcaacctg
     2641 cagcggtaca gcaaagtatt atcgaagggc tggtctggga gcaggcgaca cccacaacga
     2701 ttactcatcc acagcaactt cacgaagaac tggcgcgtat tcgccgtcag ggctggagct
     2761 atgacaacgg cgaagattac cccgatgtcc gttgtgtcgc tgcgccggtg tttaacgcca
     2821 ataacgagct taccgctgcg atctcggtgg tcggtacccg cttacaaatc aatgaagaat
     2881 accgtgatta tctggcgggc aaggccattg cctgcgccag ggatatttca cgtctgttgg
     2941 gatggaaaag tccctttgac ttacaggcct cataacaatg gagaacatca tgaccctacc
     3001 aaaaatcaaa caggttcgcg cctggtttac tggcggtgcg acagcagaaa aaggtgctgg
     3061 cggcggtgat tatcacgacc agggggcgaa tcactggatt gacgatcata ttgccacccc
     3121 gatgagtaaa taccgcgatt acgagcagtc acgccagtca tttggcatta acgttcttgg
     3181 cacgttgatt gttgaagtcg aagcagaaaa cggccagacc ggattcgctg tttcgacagc
     3241 cggtgaaatg ggctgtttta ttgtcgaaaa acatcttaac cgtttcattg agggtaaatg
     3301 tgtcagtgat atcaaactga tccatgatca aatgctcaat gccaccctgt attactccgg
     3361 ctctggtggc ctggtgatga acacgatttc ctgtgtcgat ctggctctgt gggatctgtt
     3421 cggcaaagtg gtcgggcttc cggtttataa acttttaggc ggcgcagttc gtgatgagat
     3481 tcagttctac gccacaggcg cgcgtccgga tctggcaaaa gagatgggct ttatcggtgg
     3541 caaaatgccg acgcactggg ggccacatga tggcgatgcg gggatccgca aagatgccgt
     3601 tatggtcgcg gatatgcgtg aaaaatgcgg tgaggatttc tggttaatgc tcgactgctg
     3661 gatgagtcag gacgtgaact atgccaccaa actggcccac gcttgcgcgc cctataacct
     3721 gaaatggatc gaagagtgcc tgccgccaca gcagtatgaa ggttatcgcg aactgaaacg
     3781 caacgcgcca gtcggaatga tggtcaccag cggtgagcac cacggcacac tgcaatcttt
     3841 tcgtacgctt tcagaaaccg gtatcgacat tatgcagccg gatgttggct ggtgcggtgg
     3901 tttaaccacg ctggtggaaa ttgccgcaat cgccaaatcc agggggcaac tggtggtgcc
     3961 gcacggttcg tctgtttact ctcaccatgc ggtgatcacc ttcaccaata cgccattcag
     4021 cgaattcctg atgaccagcc cggattgttc aacgatgcgt ccgcagtttg acccgattct
     4081 gctcaatgag ccggttccgg tgaatggtcg tattcataaa tcagtgcttg ataaacccgg
     4141 ttttggcgtc gaactcaatc gtgactgcaa tttgaaacgc ccctacagcc actaatcact
     4201 ccgggcgttg ctgtgacaac ggtaacgccc acatattcta aggagctttt tatgagcacc
     4261 gctttgcttg acgccgtggt gaagaaaaac cgtgtgcgtt taattccgtt tatgttggcg
     4321 ctgtatgtgc tggcgtttct cgaccgttcg aatatcggtt ttgccaaaca gacctaccag
     4381 attgataccg ggttgagtaa tgaagcttat gcgctgggag caggcatttt ctttgtggta
     4441 tatgcgtttc tgggtgttcc ggcgaatctt ttgatgcgca aactgggggc cagaacctgg
     4501 attggtacga caacactgct gtggggattt ctttcggcag ccatggcatg ggccgatact
     4561 gaagcgaaat ttctgatagt tcgcactctg cttggtgctg cggaggccgg atttttccct
     4621 ggtatgattt atctcacctc gcaatggttt ccgcagcgta atcgcgccag cattatgggg
     4681 ctgttctata tgggcgcacc gctggcgtta acactgggat caccgctttc cggcgcgctg
     4741 ttggagatgc atggatttat ggggcatccc ggctggttct ggatgtttgt gattgaagga
     4801 ttgttggcag tcggtgctgg ggtattcaca ttcttttggc ttgatgacac accggagcag
     4861 gcacgttttc tgagtaaaca agaaaaaacg ttgcttatca atcaactggc aagtgaagaa
     4921 caacagaaag tgacttctcg gctgagcgat gcgctgcgta atggccgagt ctggcaacta
     4981 gcgattatct acctgaccat tcaggtggcg gtttacggat taattttctt cctgccgacc
     5041 caggttgcgg cattgctggg aacaaaagtg ggctttacag cgtcggtggt caccgccatt
     5101 ccgtgggttg cggccttgtt tgggacctgg cttattccgc gctattccga taaaaccggc
     5161 gaacggcgta atgtcgcagc gctgacatta ctggcggcgg gcattggtat tggtctgtcc
     5221 gggctgcttt ctccagtaat ggcgatcgta gcgctgtgtg ttgcagctat cgggtttatt
     5281 gccgtgcagc cggtgttctg gacgatgccg acacaactgc tttccggtac ggcgctggct
     5341 gcggggattg gttttgtaaa cctgtttggt gcagtgggcg ggtttattgc cccgatcctg
     5401 cgcgtgaaag cagaaacgtt atttgccagc gatgcggcag gattactgac actggcagcg
     5461 gtggcggtca tcggttcgct gattattttc actctgcgtg taaatcgtac tgttgcgcag
     5521 accgacgtgg cacatcatta aataggttaa ggaacacgat gaacgcatta ttaaccaatc
     5581 cctttaaaga acgtttacgc aagggcgaag tgcaaattgg tctgtggtta agctcgacta
     5641 cttcttatat ggcagaaatt gccgctactt ccggttatga ctggttgctg attgacgggg
     5701 agcacgcgcc aaacaccatt caggatcttt atcaccagct acaggcggta gcgccctatg
     5761 ccagccaacc cgtgatccgt ccggtggaag gcagtaaatc gctgattaaa caagttctgg
     5821 atattggcgc gcaaactcta ttgatcccga tggtcgatac tgccgatcag gctcgtcagg
     5881 tggcgtctgc cacgcgctat cctccctacg gtgagcgtgg tgttggggcc agtgtggcac
     5941 gggctgcgcg ctggggacgc attgagaatt acatggcgca agtcaacgat tcgctttgtc
     6001 tgttagtgca ggtggaaagt aaaacggcac tggataacct ggacgagatc ctcgacgtcg
     6061 aagggattga tggcgtgttt attggaccag cggatctttc tgcgtcgttg ggctacccgg
     6121 ataacgccgg gcacccggaa gtgcagcgaa ttattgaaac cagcattcgg cggatccgtg
     6181 ctgcgggtaa agcggctggt tttctggctg tggctcctga tatggcgcag caatgcctgg
     6241 cgtggggagc gaactttgtc gctgttggcg ttgacacgat gctctacagc gatgccctgg
     6301 atcaacgact ggcgatgttt aaatcaggca aaaatgggcc acgcataaaa ggtagttatt
     6361 gatatcaaag gcccatgggg atcggctgtg ggcctgtgtt aattagtggt tattcgctgc
     6421 cagatcggct tcgcttagct gggtggccgc gagcacctgg tcacgatcaa tgccctgttc
     6481 cagcatgcgc aaagcaattt taatggcttg ttcatgcata ccttcctgcc agccaatttg
     6541 gtgtccttcc tgccgtagcc gctcagcaat agtcatcaat ctctcctttt gtagtggtga
     6601 acgttcggaa atctcctcaa taaaacgggt gaaacgggag gtatcgccgc attgcagcag
     6661 ataattaaac agtgtttgta gctggctgtc attagtgaag cctttaacca aaagcgtggt
     6721 gatcctgtcg accataccga ttaaatcgcg gtcgcgaata tgcttttgaa tcagttcaag
     6781 cagagctata cgccgatgtt gcatgatctc atcgtcaggt acgatggtga tatccaccaa
     6841 cggaaaagct tcggtgtaca actgccgggc aagttgcgga tcgtcaaact catccagcca
     6901 gttaagtgag tacgggtagg gcgatgtttc gccatgataa aacagcaatg gcaccaccag
     6961 cggaactctg tcatagcctt tatccaggtg acgctgcatg gcggcagtgg catagcgcat
     7021 tagccgaaaa gccatattct tttctgcaga gctttgatgt tcaatcacgc agtagatata
     7081 gccgtcaccg tcgctggttt ccacggacca caaaacatcc gagtagtaag cgcgtaaact
     7141 tttttcaata aaactagtgg gttccaggcg taaggtttgc aggttgcaaa gcttgcgcag
     7201 tggttctggc aaatgtattt cgagaaaatc ccgtgcggtt tcgggtgtga acataaaggt
     7261 tttaaatacc gcatcatgcg gggaggaggt tgttgattcg gtcatggttg tccgtcacct
     7321 gatatttatg atgacgcgac attacggtta tcccaggcct ttgacatctg ccagtttcag
     7381 cctttgcgag acgttttcag aggaattttc actgcgttgc aacgcataca ttgggcacgg
     7441 aatcgtgtac gcaaaaatgc ccggtaacca catgcttacc gggcatcaga aaggagttta
     7501 agccagcgcc tgggccagta gcgtaatcgg atgttcgcag cgaagacttg tggacatctc
     7561 aatctgccat ttacaggttt cgcagtcggt gaccaccaga tctgcgccgc tttcttctat
     7621 ctggcggaac agtggtgcgc cgatggcttg tgaggtgggg tagttctctt ttttgaaacc
     7681 gtaagtaccc gcaataccgc agcactggga atccagcacc gttaactcaa gccccgggat
     7741 tttacgcaac agctccaggg tgtagagcgt ccagcccatt ttttccatat ggcacggagt
     7801 gtgataaacc actttcagcg gcagcggttt cagcggtaac gttttgcctt cgtccagctt
     7861 gcgccacagc cagcgggttg ccagttcgat atgatcgcgc aagcctttgt tgtcgacatt
     7921 cagcacttcc gggtattcgt cgcgcagggc aaatgtacag gttgaggagg tggcaatcac
     7981 cggaatgcct tttactccca cagcttcgcg gatcgactct acattcgtaa ttgcctgttt
     8041 gcgtgcttta tcggtaaagc cgttggcgat tagcggtacg ccgcagcatt tttctttgct
     8101 gagcagttgt acaccgatac ccattgcgtt gagcacttta attaaatctt tacctaactg
     8161 cggatggttg tagttaacga agcagccgtg aaagaaagcg acctggtctt tatattgtgc
     8221 ttgctgagcc gctatgctgc gataccagcg acggaacgtg ccgaaggagt atttcggtag
     8281 cgtgcgacga tgatcgattt ttaacgccgc atcaagcagc tgccgcaccg gtttcagcga
     8341 ggtagcggtg ttgacgattg gtgcgaacgg cgtggaaacg ctacccatca ggtcggtatg
     8401 actcaacaca aaattacgca gcgacgggcg cgtggtgtca tattttgccc gcgcgcgctg
     8461 gataatatcg ccaatcttca catcggacgg gcaggcgact tcacaacgtt tgcagttgat
     8521 gcaatatttc agcgcctcgt catacagtgc gccatctttc aaacgcagac gctcgccatc
     8581 cggcccggct tgttttggcc ctggataacc gggattcacc cggctcaccg ggcaggcggt
     8641 ggtgcagacg gtgcacttaa tgcagttttc gaagctggtg tcgttcattg ttggcctcct
     8701 gcgcgttggg caatctgttg agcggcatgt aaagcactga cggcacaaac accgccgccg
     8761 catccctggg cgatgggatc aaatccgccc agcaccgaac cgatggcaaa caggttctca
     8821 atggtttgcc ctgcctgcga cgggcgtagc gtctcatcag tggttacacc gaactgctgc
     8881 cacggttgcg gcgcaaaaaa atcgccctta taccattcac cccgcgtggc ggtttgtagc
     8941 acatcaaggc cgagaatcgg ctcgcgaatg ccgttacgtt ccgctaccag tccgccacta
     9001 aagaaactgc cgctggcgag aaccgcgaaa cgtggacgta gcggaatatc ggcgtgattg
     9061 cgggtccaga tttcattcac tacgccattt ttacaggtca cttttttcac ttcatcgccc
     9121 ggcatccaca cgccaccctg gcgcacaaac tggcgctgta actggttttg cagacgaatg
     9181 cccagcacgg aaggcggcag cgttggcaaa agcatcagtg aacaaggtag tttttcattc
     9241 aaccaacgcc acagtttgtc atcggccagg ccgaagcagg cgggcatcag gatcatttcg
     9301 caggtattgg cgacaggaat aagcgcatca agtaacagcg gccagttttc ttcattatca
     9361 aggaaacggg cgatattcac cgcgcgaaat tcggtggcgt tattgcgcag gacatccagt
     9421 tccggcagct ctatttctgc ggtttcaacc gaaagatcca gttcgcgcaa cgaagccgcc
     9481 gcaagatgcg cctggaaatc catcagcccg cttatcccca ctacacatat cttcttcgcg
     9541 ggtagcggcc agacgggaac ttctggcgaa cttaaccagg ttgcacgcaa agtgccgagc
     9601 ggggtaacgc gctgatgggc cagttccacg ctgccctgca attgcgcacc gctttcagcg
     9661 atcaatgcct gcgcctggca agcgagatcg agcacgcgtt gtggttcgag aagactgtaa
     9721 ggatgggctg gtgcctgctg acgcaaagat tccagtccac tgtgaatgtc tgtcaccggt
     9781 tgaccatcag gcagatggct cagcaaatcc agcgaaccgg atgagaaatg cagcgcgctt
     9841 tgaccacgcg tgacaatggc acagcgcagg ccgtgttttt gcagttgcag gccacagagt
     9901 aatccggcga ggccgccgcc cataatgaca gtatcaaagc gcatctttct gctccttctc
     9961 cagaccacac agtccctgat aaacccagcg ggtaaattcg ctttcgcgca gtgcatctcc
    10021 ccaggcgatg ggttgcacgc ctttccagcg ttcgttaagg aaggtggaaa gttgctcgat
    10081 agattgcgcg gacgtcgtga cgttaaaacg ttgcagcagt ccggcagcgc ggcaggcgca
    10141 gagttcgccc tggcaggtac ccatccccac acgggtacga cggcgtaaat ccagcaggct
    10201 attaacgttt aaattttcta ccgcgtactg cacttcaccc gctgttaccg cttcgcactc
    10261 acataccagg ctacggtgca gacgaccttc gctcagccag gcaggcgtgc gatcgccatg
    10321 acgataaacc gcagaaccgc gcagcggggc aggcagggag atgactttac gcaaggtaac
    10381 ttcagcgggt tcctgtgaac caggaagtgc cagatcggcg gtcgtacagg ggcgcgtgtt
    10441 gcccagtttg cggcacacgg cgtcggtagc ccattcagcc atcagccgat aggtcatcag
    10501 tttgccaccg gtgatggtga taaatccgtc cagaccatcg cgttcagcat ggtcgagcag
    10561 cacgatgcca cggctgacgt tacgtccgct cgggtcgtca tcgctggcaa ccagcgggcg
    10621 cacgccagaa taggcccgca aaatgcgcgt tttcgccatc acgggggcca gtttttcccc
    10681 ttcacgcagc agaatatcaa cctcttctgc cgtcactcga ttatcgtcaa tctcgttgta
    10741 atcaatacgt aaagaggtgg tgccaatcag cgaaatggta tcgccaggca ccagaatatc
    10801 ggcgtcggaa ggtttacggc agcggttgat cacatgctgg ttaatgcggt gatccatgat
    10861 cagcagcgat cctttcgccg ggaacatgcg aatgcgcaga tcggcatatt cggcaatgtg
    10921 ttgcccccag atcccggcgg cattaaccac gacaggcgca tgaagggcct gagtttcgcc
    10981 ggtgagatgg ttacgtacac gaacaccgca caccgtcgcg ccttcacgaa tcagtcccgt
    11041 gacttcatga gcggtaagga taacggcacc gtgttctttg gcatccagca tgtttgctgc
    11101 ggtcagacga aatggatcaa cggtgccatc cggaactttc accgcgccaa tcaatgccgg
    11161 gttaacggca ggttcgataa tgcgcgcttg ctgcgggtct atagcttctg cgctgatccc
    11221 tgcttcttcg caggcgcgaa taaaagtggc ctggaaggag aggtcatctt ccggcagggt
    11281 aataaacagg ccgttggttg gttcaacgca gtgacgtgca atgcgtttca ggatctggtt
    11341 ttcactaatg cattcgcggg ccgattccgc atcggttacc gcatagcgcg caccgctgtg
    11401 tagcaggccg tggttacgcc cggtggcacc ggttgcgatg tcgtggcgct caaccaaaat
    11461 cacgcgcagc ccgcgcaggg cacagtcgcg ggcaatcccg gctcccgttg cgccgccgcc
    11521 aatgataatc acgtcacttg attgcgagtc gcgagttttc attgttatcc ctctgaagtt
    11581 cgttttttac catttagcca tagtaaaaac atgaattgtt tgatttcgcg catattcgct
    11641 catgattcga aagtgaaacg tgatttcatg cgtcattttg aacattttgt aaatcttatt
    11701 taataatgtg tgcggcaatt cacatttaat ttatgaatgt tttcttaaca tcgcggcaac
    11761 tcaagaaacg gcaggttctc tcactgaatc aggctgttaa tcataaataa gaccacgggc
    11821 cacggaggct atcaatgttg agtattttta aaccagcgcc acacaaagcg cgcttacctg
    11881 ccgcggagat cgatccgact tatcgtcgat tgcgctggca aattttcctg gggatattct
    11941 ttggctatgc ggcttactat ttggttcgta agaactttgc gcttgctatg ccttatctgg
    12001 ttgagcaggg attctcacgc ggtgatttag gttttgccct ttcggggatc tcgattgctt
    12061 atggattttc gaaattcatc atgggttcgg tatcggatcg ctcgaatccg cgcgttttcc
    12121 tgcccgcagg tttgattctg gcggcggcag tgatgttgtt tatgggcttt gtgccatggg
    12181 cgacgtcgag cattgcggtg atgtttgtac tgttgttcct ctgcggttgg ttccagggga
    12241 tggggtggcc gccgtgtggt cgtactatgg tgcactggtg gtcgcagaaa gaacgtggcg
    12301 gcattgtgtc agtgtggaac tgtgcgcaca acgtcggtgg tggtattccg ccgctgctgt
    12361 tcctgctggg gatggcctgg ttcaatgact ggcatgcggc gctctatatg cctgctttct
    12421 gcgccattct ggtggcatta ttcgcctttg cgatgatgcg cgataccccg caatcctgtg
    12481 gcttgccgcc gatcgaagag tacaaaaatg attatccgga cgactataac gaaaaagcgg
    12541 aacaggagct gacggcgaag caaatcttca tgcagtacgt actgccgaac aaactgctgt
    12601 ggtatatcgc catcgccaac gtgttcgttt atctgctgcg ttacggcatc ctcgactggt
    12661 caccgactta tctgaaagag gttaagcatt tcgcgctgga taaatcctcc tgggcctact
    12721 tcctttatga atatgcaggt attccgggca ctctgctgtg cggctggatg tcggataaag
    12781 tcttccgtgg caaccgtggg gcaaccggcg ttttctttat gacactggtg accatcgcga
    12841 ctatcgttta ctggatgaac ccggcaggta acccaaccgt cgatatgatt tgtatgattg
    12901 ttatcggctt cctgatctac ggtcctgtga tgctgatcgg tctgcatgcg ctggaactgg
    12961 caccgaaaaa agcggcaggt acggcagcgg gctttaccgg gctgtttggt tacctgggcg
    13021 gttcggtggc ggcgagcgcg attgttggct acaccgtgga cttcttcggc tgggatggcg
    13081 gctttatggt aatgattggc ggcagcattc tggcggttat cttgttgatt gttgtgatga
    13141 ttggcgaaaa acgtcgccat gaacaattac tgcaaaaacg caacggaggc taatggcatg
    13201 aaattgaagc tgaaaaacct tagcatggcg atcatgatga gcactatagt catgggaagc
    13261 agtgcaatgg cggcggacag caacgaaaaa atagtcatcg cccatcgcgg tgccagtgga
    13321 tatttgccgg agcatacgct gccagcaaaa gcgatggcgt atgcgcaggg agcggattat
    13381 ctggaacagg atttggtgat gaccaaagac gaccatctgg ttgttctgca tgaccattat
    13441 ctcgatcgtg ttactgatgt tgccgatcgt ttcccggatc gggcgcgcaa agacggtcgt
    13501 tactacgcga tagatttcac gctggatgaa attaagtcgc tgaaatttac cgaaggtttc
    13561 gatattgaaa acggtaaaaa agtacagact tatccggggc gtttcccaat gggtaagtcc
    13621 gacttccggg ggcacacctt tgaagaagag attgaatttg ttcaggggtt aaatcactct
    13681 accgggaaaa atatcggtat ctatccagaa atcaaagcgc cgtggttcca tcatcaggaa
    13741 gggaaggata ttgcggcaaa aacgctggaa gtgctgaaga aatatggtta caccggtaag
    13801 gacgataaag tttatttgca atgttttgat gctgatgagc tgaagcgtat taagaatgag
    13861 ctggaaccta aaatgggcat ggatctcaat ctggtacagc tgattgccta taccgactgg
    13921 aatgaaacgc agcagaaaca gccggacgga agctgggtta attacagcta tgactggatg
    13981 tttaagccag gtgccatgaa gcaagtcgcg gaatatgccg acggtatcgg gccggattac
    14041 catatgttga ttgaggaaac atcgcagccg ggtaatatca aactcactgg catggtgcaa
    14101 gatgctcagc agaataaact ggtagtgcat ccttataccg tgcggtcgga taaactgcct
    14161 gaatacacta ctgatgtgaa tcagttatat gatgctctgt ataacaaagc gggtgtaaat
    14221 gggctgttta ctgatttccc tgataaggca gtaaaattcc ttaataaaga gtaatgctac
    14281 tgcaggatgg cgttcatgcg ccatcctgtc tcatatcaaa tttttccaat cgccactaat
    14341 tgttccatgc acatttgtag taaaaacagt ggactgcctt ctaccggctt ttctttagcc
    14401 aacaacgaaa tctgtcgata gaaggttggt tcgtgaggaa cggaacacaa ttcacctgca
    14461 atggttttca gcgttaactc tggtaataag gcgatgccta atccctgtcg aataaaattc
    14521 atcgctgtat ccacctgatt aaattcacat tttatctgct gggcgcacta ttagtggtcg
    14581 tggctggtgg aatctatgcg ctgcaaatga agggcaaaag cggtgtcaca ctagtagcgg
    14641 caaaagaaat tcattaatac gacacgtttc attaagattt tcctcaggta accacctata
    14701 gtcattgtcg ttaataaaaa tgacaggcga tagggtatgg cagtttcagc aaagtacgac
    14761 gaatttaatc actggtgggc aacagagggc gactgggttg aagagcctaa ctatcgccgt
    14821 aatggtatga gcggggtgca atgcgtcgag cgcaacggca aaaagctgta tgtaaagcgc
    14881 atgacgcatc atctgtttca ttccgtacgt tatccgttcg gccgaccaac gattgtccgt
    14941 gaggttgcgg taattaaaga actggaacgg gccggtgtca tcgtgccgaa gatcgttttt
    15001 ggtgaagcgg tgaaaattga gggtgaatgg cgcgcgttgc tggtgactga agatatggcg
    15061 gggttcatca gcattgctga ctggtatgcc cggcatgcag tatcgcccta ttctgacgaa
    15121 gtacggcaag ccatgttgaa agcagtggcg ctggcgttta agaaaatgca tagcgttaat
    15181 cgtcagcatg gctgttgtta tgttcgccat atctatgtga aaacagaagg taaagcagaa
    15241 gctggttttc ttgacctgga gaaaagtcgt cgccgcttgc gtcgcaataa agcgattaat
    15301 catgacttcc gccagctgga aaatatctgg agccaatccc caaagcggac tgggagcagg
    15361 tgaaagcgta ttactacgcc atgtaatcgt tgtaggccag ataagacgta taagcgttgt
    15421 atctggcact acaaccaatt cacatctcga tttcaatatc gccttttgcc cggcaacaac
    15481 agggcaaaat ttcccccggc tgaataaagg ctaacggttc ggcaatccag tccacctgac
    15541 ctgcgaccag tcgggtgcga caggagccgc agtaaccttc gcggcactgg tactcaaccg
    15601 caacattgtg ggattccagc gccgccagaa gggaagggtg ttcatcctgg cacagcagtt
    15661 gtgtgccagt gatgcgcagg gtaacgcggg ccatcagagc tggaagttac tcaaatcgtc
    15721 ggtgtccact tccgcgtcaa tctgcccgac cagataagaa ctgacttcca cttcctgcgg
    15781 agcaacctgc acgttatcag acaccagcca ggtgttgatc cacgggatcg ggttggagcg
    15841 cgtctggaac ggcagatcca aaccgactgc ctgcatgcgg atattggtga tgtattcaac
    15901 gtactggcag agaatatctt tattcagacc aatcatcgaa ccgtcacgga acagataatc
    15961 cgcccagtct ttctcttgct gtgccgcctg gacaaacagg tcatagcact cctgcttaca
    16021 ttcttcggca atttccgcca tctccggatc atccgcgccg ctgcgcagca gattcagcat
    16081 atgctgagta ccggtcaggt gcagggcttc atcgcgggca atcaggcgaa taatcttggc
    16141 gttaccttcc atcagttcgc gttctgcaaa ggcaaaggaa caagcaaagc tgacgtagaa
    16201 gcggatcgct tccagcgcgt taacgctcat caggcagaga tacagttttt tcttcagctc
    16261 gcgcaggcta acggttacgg ttttaccgtt aacggtgtgg gtgccttcgc ccagaaggtg
    16321 ccagtagctg gtcatttcga tcagttcatc gtaatagctg gagatccctt ccgcacgttt
    16381 ctggatctgc tcgttggtga cgatatcgtc aaacacaaca gacggatcgt taacgatatt
    16441 acgaatgata tgagtatagg aacgggaatg aatcgtttct gagaacgccc aggtttcgac
    16501 ccaggtttcc agttccggaa tagaaataag cggcaatagc gccacgttcg ggctacgacc
    16561 ctgaatggaa tccagcagcg tctgatattt caggttgctg ataaagatgt gtttttcgtg
    16621 ctccggcagc gcctggtaat ctatacggtc gcgggagacg tcaacttctt ccggacgcca
    16681 gaagaaagag agctgctttt cgatcagctt ttcgaagatg tcatattttt gctgatcgta
    16741 gcgagccacg ttgaccggct gaccaaagaa catcggttct ttgagctgat catttttcgt
    16801 ctgtgaaaag gtggtatatg ccatgagtgt gtcctgttgg gagtttaatg ccggataagg
    16861 cattttacgc cgcatcctgt gcctgatgcg acgctggcgc gtcttatcag gcctacaaat
    16921 ccgagccgta ggccggataa ggcgttcacg ctgcatccgg cacccggagc ctgatgcgac
    16981 gctggcgcgt cttatcaggc ctacaaaccg agccgtaggc cggataaggc gtttacgccg
    17041 catccggcat ctcaatatca gatcttacat gcgccgcttt cgcagccatc gtcctggatt
    17101 gacggtacca gatcgtcctg cgcgtcttca gcgccgtcac gggtgttctg ataatacagc
    17161 gttttgaccc cgaatttgta ggcggtgagc aggtctttca gcaactgctg catcggcact
    17221 tttcctgacg ggaaacgtga cggatcgtag ttggtgttag cagagatcga ctgatcgata
    17281 aatttctgca tgatacctac cagttgcagg tagccatcgt tacccggcat ttcccacagc
    17341 agctcatagg cgtcgtgcag gtgctcgtag tccggcacca cctggcgcaa aataccgtct
    17401 ttcgacgctt tgatgctgac gtaaccgcgc ggcggttcaa taccgttagt ggcgttagag
    17461 atctgcgaag aggtctcgga cggcatcagg gcagaaagcg tagagttacg caggccgtgc
    17521 gttttgattg attcacgcaa tgcttcccag tcgtaatgca gcggctcatt agcgatggta
    17581 tccagatctt tcttataggt atcgatcggc aggatccctt tcgcgtaagt ggtttcgtta
    17641 aaccacgggc acgcgccttg ctctttcgcc agctcattag aggctttcag caggtaatac
    17701 tgaatggctt cgaaggtttt atgcgtcagg ttgttggcgc tgccgtcgga gtagcgttta
    17761 ccgtgcttcg ccaggtagta agcgaagttg atcacaccaa tacccagcgt acgacgaccc
    17821 atcgctccac gtttggcggc cgggatcggg taatcctgat aatccagcag cgcgtcaagt
    17881 gcacgaaccg ccagaattgc cagctcttcc agttcatcca ggttattaat tgcgcccagg
    17941 ttgaaagcag acagcgtaca cagcgcgatt tcaccgttct cgtcgttgac gtcgttcagc
    18001 ggtttggtcg gcagggctat ctccaggcac aggttagact gacgcactgg cgcgatggcc
    18061 ggatcaaacg ggctatgggt attgcagtgg tcaacgttct gaatatagat acgaccggta
    18121 gacgcacgtt cctgcatcat cagcgagaac agctcaacgg ctttcacacg ctgcttgcgg
    18181 atgctgtcgt ctttctcata tttggtatac agacgttcaa actcttcctg atcggcgaag
    18241 aacgcgtcgt acagccccgg tacgtcggac gggctgaaca gggtgatatc ttcacctttc
    18301 agcagacggg tatacatcag tttgttgatt tgtaccccgt agtccatatg acgcacgcgg
    18361 ttgccttcca caccacggtt gtttttcaac accagcaggc tttccacttc cagatgccac
    18421 atcgggtaga acagcgttgc cgcaccgccg cgcacaccgc cctgagagca ggatttcacc
    18481 gctgtctgga aatgtttgta gaacggaatg cagccggtat ggaacgcttc accaccgcga
    18541 atcgggctac ccagcgcacg aatacgcccg gcgttgatgc cgatcccggc acgctgggaa
    18601 acgtatttaa caatcgcgct ggaggtggcg ttgatggaat ccaggctgtc accgcactcg
    18661 atcagtacgc aggagctgaa ctgacgagtc ggggtacgca cgccggacat gattggcgtc
    18721 ggcagcgaaa ttttaaatgt ggaaaccgcg tcgtaaaaac gcttcacata ttgcaggcgc
    18781 gtttcacgcg ggtagttcga gaacaggcac gcggcaacca gaatataaag gaactgggcg
    18841 ctctcataga tttcgccggt cacgcggttc tgtaccagat atttgccttc cagctgctta
    18901 acggcagcat aagagaaggt catatcacgg tcgtgatcga taaacgtgtc catctgcttg
    18961 aactcttctt ccgtgtagtc ttccagcaga tgattatcgt atttgcccat ctcgaccatt
    19021 ttcaccacgt ggtcgtacag cgcaggcggc tcaaactggc cgtaggcttt tttacgcagg
    19081 tggaagatcg ccaggcgcgc ggcgagatac tgataatccg gcgcatcacg ggagatcagg
    19141 tctgcggcag ccttgataat ggtttcgtgg atgtcagagg tcttgatacc gtcataaaac
    19201 tgaatgtggg agcgcagctc gacctgggaa atcgaaacgt tatgcagtcc ttctgccgcc
    19261 caatccagaa cgcgatggat tttgtcgaga ttgatgcgct ctgtgctacc gtcgcgcttt
    19321 gtcaccagca gattctgatt catgtatgtc gtacctgttt ttggaattct ttccgcaata
    19381 gtttcatgca gactacctgt agtaagggaa gctgcttaga acactatata tagggggtat
    19441 gtttgggatt cactgcaaga tagtgtgaaa atgaccctct tgcaagtgca taactttgtg
    19501 gataactcag gaaggaaaaa gtggctttcg cgcaccttag gtcagacaag gtgtccggga
    19561 aagtcaatgg gaagagaaaa atttgttaaa aataactgtt cggcgatttc ttgaacggca
    19621 gaataggttg gataaattga tatagatggt ttattaatct ttttgttaat gattgcaaaa
    19681 attgatctaa gcacgagtca tcacataatc tgatgtatca gttgtggtca atatataagc
    19741 gaatgtaagc gattgttgat gtcgaaatgg ttgttttcta ttcggttatt gttaaatgcg
    19801 aagccatttt gttaatttac cttatattat aagaaaatta ttcattacta taatttagtt
    19861 taaatataac tggtatccct taaggttatt aacttgaatt atgacgcact gatattattc
    19921 atcaaataat aacaaaatag ccattgcatc gggttggaca atttacaaaa gaaagataac
    19981 ccatattgta taatgcgttt aaatatgccg tcttatataa aatgatggtc agattaatat
    20041 gcggattatc tttctacgca aggagtattt atctctactc ccgtcaatga ttgcatctct
    20101 tttctctgct aacggtgtcg cggcggccat tgatttatgc cagggatatg atatcaaagc
    20161 gagttgtcac gccagcaggc aaagcctttc aggcattacg caggactgga gtatcgccga
    20221 tgggcaatgg ctggtttttt cggatatgac caataatgcc agcggtgggg ccgtattttt
    20281 gcaacaagga gcggaattta cattatcacc agaaaacgaa actggaatga ctctgtttgc
    20341 caataacacc gttacaggag aatattataa cggcggggcc atatttgcta aagaaaactc
    20401 aacgctgaat cttactgatg tcattttttc cggtaacgtc gcaggcggct atggtggcgc
    20461 aatctattct tctggtacta acgatactgg tgccgtcgat ttaagtgtca ctaacgccat
    20521 gtttcgcaat aatatcgcta atgatggcaa aggtggcgca atttatacca ttaataatga
    20581 cgtctattta agtgatgtta tttttgataa caaccaggca tatacatcaa caagttacag
    20641 tgatggcgat ggcggggcaa tcgatgttac cgataataat agcgacagca agcatcctcc
    20701 aggttatacg ataataaata acactgcctt tacaaataac actgccgaag gttatggcgg
    20761 ggcgatatat accaatagcg cgacggctcc ctatcttatt gatatttctg ttgatgacag
    20821 ctacagccag aacggcggcg tgttagtgga tgagaacaac agcgcagcag gctatggaga
    20881 tggtccttcc actgcggcgg gtggctttat gtatctcggc ttaagtgaag ttacctttga
    20941 tatt
//
LOCUS       DAZOZY010000055        20828 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZOZY010000055 DAZOZY010000000
VERSION     DAZOZY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20828
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.055"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(21..1880)
                     /gene="gss"
                     /locus_tag="ACHURQ_001797"
     CDS             complement(21..1880)
                     /gene="gss"
                     /locus_tag="ACHURQ_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR0823593.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2085..2951
                     /gene="yghU"
                     /locus_tag="ACHURQ_001798"
     CDS             2085..2951
                     /gene="yghU"
                     /locus_tag="ACHURQ_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR0823594.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2980..3061)
                     /locus_tag="ACHURQ_001799"
                     /pseudo
     CDS             complement(2980..3061)
                     /locus_tag="ACHURQ_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3074..3322)
                     /gene="hybG"
                     /locus_tag="ACHURQ_001800"
     CDS             complement(3074..3322)
                     /gene="hybG"
                     /locus_tag="ACHURQ_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR0823595.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3335..3676)
                     /gene="hypA"
                     /locus_tag="ACHURQ_001801"
     CDS             complement(3335..3676)
                     /gene="hypA"
                     /locus_tag="ACHURQ_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR0823596.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3669..4157)
                     /gene="hybE"
                     /locus_tag="ACHURQ_001802"
     CDS             complement(3669..4157)
                     /gene="hybE"
                     /locus_tag="ACHURQ_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR0823597.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4150..4644)
                     /locus_tag="ACHURQ_001803"
     CDS             complement(4150..4644)
                     /locus_tag="ACHURQ_001803"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR0823598.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4644..6347)
                     /gene="hybC"
                     /locus_tag="ACHURQ_001804"
     CDS             complement(4644..6347)
                     /gene="hybC"
                     /locus_tag="ACHURQ_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR0823599.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6344..7522)
                     /gene="hybB"
                     /locus_tag="ACHURQ_001805"
     CDS             complement(6344..7522)
                     /gene="hybB"
                     /locus_tag="ACHURQ_001805"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR0823600.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7512..8498)
                     /gene="hybA"
                     /locus_tag="ACHURQ_001806"
     CDS             complement(7512..8498)
                     /gene="hybA"
                     /locus_tag="ACHURQ_001806"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR0823601.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8501..9619)
                     /gene="hybO"
                     /locus_tag="ACHURQ_001807"
     CDS             complement(8501..9619)
                     /gene="hybO"
                     /locus_tag="ACHURQ_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR0823602.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9808..10095)
                     /gene="yghW"
                     /locus_tag="ACHURQ_001808"
     CDS             complement(9808..10095)
                     /gene="yghW"
                     /locus_tag="ACHURQ_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR0823603.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10214..11101)
                     /gene="yghX"
                     /locus_tag="ACHURQ_001809"
     CDS             complement(10214..11101)
                     /gene="yghX"
                     /locus_tag="ACHURQ_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR0823604.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11258..12298
                     /gene="gpr"
                     /locus_tag="ACHURQ_001810"
     CDS             11258..12298
                     /gene="gpr"
                     /locus_tag="ACHURQ_001810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR0823605.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12338..12832)
                     /locus_tag="ACHURQ_001811"
     CDS             complement(12338..12832)
                     /locus_tag="ACHURQ_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR0823606.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13023..13907
                     /gene="yghA"
                     /locus_tag="ACHURQ_001812"
     CDS             13023..13907
                     /gene="yghA"
                     /locus_tag="ACHURQ_001812"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR0823607.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14067..14492)
                     /gene="exbD"
                     /locus_tag="ACHURQ_001813"
     CDS             complement(14067..14492)
                     /gene="exbD"
                     /locus_tag="ACHURQ_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR0823608.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14499..15233)
                     /gene="exbB"
                     /locus_tag="ACHURQ_001814"
     CDS             complement(14499..15233)
                     /gene="exbB"
                     /locus_tag="ACHURQ_001814"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0823609.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15485..16672
                     /gene="metC"
                     /locus_tag="ACHURQ_001815"
     CDS             15485..16672
                     /gene="metC"
                     /locus_tag="ACHURQ_001815"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR0823610.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16812..17471
                     /gene="yghB"
                     /locus_tag="ACHURQ_001816"
     CDS             16812..17471
                     /gene="yghB"
                     /locus_tag="ACHURQ_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR0823611.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17511..18410)
                     /gene="yqhC"
                     /locus_tag="ACHURQ_001817"
     CDS             complement(17511..18410)
                     /gene="yqhC"
                     /locus_tag="ACHURQ_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR0823612.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18604..19767
                     /gene="yqhD"
                     /locus_tag="ACHURQ_001818"
     CDS             18604..19767
                     /gene="yqhD"
                     /locus_tag="ACHURQ_001818"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR0823613.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19872..20699
                     /gene="dkgA"
                     /locus_tag="ACHURQ_001819"
     CDS             19872..20699
                     /gene="dkgA"
                     /locus_tag="ACHURQ_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR0823614.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
BASE COUNT         5122 a         5608 c         5228 g         4870 t
ORIGIN      
        1 cggggttgct acaacatgta ttactttttc accacaatta acggttcaat atcactctct
       61 tttttgatga ccagtgattc atcaccgcgc aaacacgtcc caccgtagtt gccgcccacg
      121 gtgaaggtac atacctgaat gtatttaccg tccactttcg gcaaacacca cagttgctga
      181 tagatgtttt tctgctcggc aaatttaccg ctggttttgt ccagcacctc ttcatgatgg
      241 ctgaccaggt cgatattgct accacagcga ccggcaatcg gtttcactgc gtaacctgtt
      301 ttcaccaatt catcattaac agtgaaatcg gtatccagca ggtaacgatg gtgcgggaac
      361 agcgaccaga ggatcggcag aatcgctttg ttgccgggga tcaccgtcca cagcggctca
      421 aagactagca cttccgggcg cagcaatacg tcgataagac gcacttcgtt ttgcggatga
      481 ccggtacgga ttggcaccgc agcaaactca cggtcgctaa cttcacgaat ctgatcaaac
      541 gcggtttccc acgcccaggt tttccacacg cagttaacca gtcgcccttc cccatcaatc
      601 agttgcccgg cagcatccca gcccagttca tccaatccac gcaagatacg cgtttcaaag
      661 cccgcctggt gcagcgcctg ctccataaac tgcgcgtgat agttttcctc gatatctttg
      721 tcctgcatga tatggacaaa cggacgtgca cgactgtgtt tccaggcacc agccaattcg
      781 ttaatcagcc cttccgccgg attgaagccg ttgcctttat agccctgctc cgcccaacgt
      841 tcgaggatca agcccgcttc ggtatgacag gaggcggagt cggcgttgta ctcgtaaacc
      901 ttcaggccac gctcatccat gcagaaatcc atacgaccag tgatcatatg gtgacggcga
      961 cgctgccagg agagacgcaa acgtggccag aggattttcg ggatgtcgaa cagcgccagc
     1021 aggttgtcat ctttcagcac cttgtcggtt gcgtgaagat acatcaggtg cagctcgttg
     1081 gtggctttaa ttagctcctg ctcggcactc tcggtaatgg tgtagtaatg ataaggatcc
     1141 tgattgatca cctgaccgtt ggcctgcaca taggcgtttt gcagcggatc tttttcatcc
     1201 agccatttac cgtcaaactg gcctttgttt tccaggcgcg ctccgctgat tttcagcagc
     1261 tcgcctgcaa tttccggctg cggtaagctg tattcagtat cttccgtctg gatcatccag
     1321 cccagaatgg tggtgtcatc aaaagtgtct ttcagggtat agcagccgtt ttcgaccacc
     1381 atctccagct cacgcgtcca ctgttgccct tgcggcaacg gggaatgaat cacgttctgt
     1441 tccgcaatac ggactttgtt gccatgcaat tgggtaatga tggcgacatg gccagtgtct
     1501 ttaaattcac cgcctttatc ccagataaga agcgcacccg cgaccggcgc acgcggcgag
     1561 ccgttaggaa atgcctgcaa tggcaggatg ttgtcattaa ccacttctcg caggaagcgc
     1621 agcgagaaaa tctcccacgc catacccacg tcagtaaaga ccacaccgta attcagaaag
     1681 agaaaacggc gagcaaattc aacgcattgc cacttgtggc ccatatattc gtcgtcgata
     1741 tagctacgga atacggcgtc atcttcgtat tcctgcggat cgagagaact gtaatctgaa
     1801 gagtagattg ctaccccacc tggggcgtag cccaataatg tcccgaacgg ggcatcctgg
     1861 ctggtcgttc ctttgctcat cactttacct ttaacaatac aacctaagca gttggcgtaa
     1921 ttgttgtgct ctgattacct gctcagcgac attaaccgga cagaggtcag actaatcata
     1981 cacctcatcg cagcggctgg cgcaaagggt taaaaaattc acattctgta cagcaagtga
     2041 cctgctacac tgcttcaaca ctaccactca gaaggcaact cactatgaca gacaatactt
     2101 atcagcccgc gaaagtctgg acgtgggata aatccgctgg cggcgcgttc gccaatatca
     2161 atcgcccggt ttctggtccg acgcatgaaa aaacgctgcc cgttggcaaa cacccattgc
     2221 aactttattc gctgggaacg ccgaacggtc agaaagtaac gattatgctt gaggagctgc
     2281 tggcgctggg cgttactggt gcagagtacg acgcctggct gattcgtatt ggcgatggcg
     2341 atcaattctc cagcggcttt gtcgaagtga acccaaactc gaagatcccg gcgctgcgcg
     2401 atcatacgca taatccgccg atccgcgtgt ttgaatctgg ttcgatcctg ctttatctgg
     2461 cggagaaatt tggctacttc ctgccgcagg atttggcaaa gcgtactgaa acgatgaact
     2521 ggctgttctg gttacagggc gcggcaccgt tcctcggcgg tggttttggt cacttttacc
     2581 attacgcgcc gatgaaaatt gagtacgcca tcaaccgctt taccatggaa gccaaacgtc
     2641 tgctcgacgt gctggataag caactggcgc agcataagtt tgttgcgggc gatgagtaca
     2701 ccattgcgga tatggcgatt tggccgtggt ttggcaatgt ggtgttaggc ggcgtgtatg
     2761 atgccgcaga gtttcttgat gcgggcagtt ataagcatgt acaacgctgg gcgaaagaag
     2821 taggcgaacg tccggcggtg aaacgtgggc gtattgttaa ccgcaccaac ggaccgctga
     2881 atgagcagtt gcatgagcgc catgacgcca gtgatttcga gacgaatacg gaagataagc
     2941 gtcaggggta agggttggtg ttcgtcgcag caagccatcc aggccggata aggcgttcac
     3001 gccgcatccg gcaatcgtgc acaatgcctg atgcgacgct gccgcgtctt atcaggccta
     3061 cgaaagctaa tcatcacgcg ctggtaatgt cgtaatccat ttggcgcagt gcgtctaatg
     3121 tggctttggc ttcatcttcg tcgatgatgc tcatggcaaa tccgacgtgt accagcaccc
     3181 actggcccag tagatcggca gggttacctt cacaaatcag ggcgatattc acgtcgcgct
     3241 tgataccaca tacttcaacc tgcgcaagct ggtgaatatc ttcaccgaca gccagcacct
     3301 ggcctggaac gccaatacac attattaact ccggttattc aacttcaata cttttgacga
     3361 tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga cactgcgcat
     3421 cgtgctgatg aatctccacc acctggctgc aatcccagca ccaagcctgg gcgggtttat
     3481 agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt tcaaaactaa
     3541 aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac acggcggtga
     3601 cgcgcttaac atcgtgctgc tccgcctgcc gttggataat ttcaacggcg ctctggcaaa
     3661 gagacaactc atgcattttc gccactcctg cgaccaaaca gcagggcgcg acgccctgcg
     3721 tgtggtacat ccggattcgt gactggcagc gaaaggatca ttcgtgcgca gtcatccgtc
     3781 aggcgttggc cctcttcaat cgacatgctg tgcgaaagcg gcgacatcag cgaacaggag
     3841 agatattgcg aaacaccgtc cagttcgcca acggtaaagg tcatagtgcc atacggcagt
     3901 tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg gccggggaag
     3961 ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc ttcgaacagc
     4021 gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagatc gtgcatcgaa
     4081 cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt ctggaaacct
     4141 gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgcctg attcgcgcag
     4201 cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg gcgttaagcc
     4261 gatgtgtggc tccagcgatt ccgggatcac gccgaccagg gtcagctttt tcggaaactc
     4321 accggtgaag cgcagggccg acaatacgtc ggccaggcca agctgatgcg gagagatttt
     4381 gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc ccggcgtgtt
     4441 ctttttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca tgtcgccaag
     4501 cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca gaatgtatcg
     4561 ttgctctaac gcttcgacaa tccgcacacc gatggcttca tcggtcagca aaatattgcc
     4621 gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc gttgccgtcg
     4681 gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat ggtacgcacc
     4741 acttccagcg gtttattcgg atcagcaacc ggtgtaccca ccagcgactg ctcgtaagga
     4801 ccgacgtcat cattgaagtt acgcggacca gagttccagg ttgatggaac aactgcctgg
     4861 tagttactga taataccgtc tttaataacc atccagtgag agagcatacc gcgcggcgct
     4921 tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggttt cacaaaggtg
     4981 gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attgattttg caggatatcc
     5041 tgcaattcgc agcagtgaac ggtacgacca ataatacggc ccagcgtgga gtgcagctgc
     5101 gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc attcagtttg
     5161 ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag cggacccact
     5221 tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc gtcgtcagac
     5281 caaccatcat aagccggaat ggtggtacct tcccacggtg cctgcggcgc ttcgtcttta
     5341 taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc ggaatgagaa
     5401 gtgatcggac gatacgagga cagatccgca ttctcaatgt agccgcccgg gaacaggaag
     5461 ctgccgtttt tactgtcggt cgggaattcc ggcacgctca ggtagttcac cgcaccttta
     5521 ccgcgcgtca gccattccgg gtagaacgcg gcaataactg cggtatcaac cttataaacc
     5581 tgttcaacaa agtcgctcag cttgtcgatg aaagacttga tgtacatcag gcgctcaagg
     5641 ttcagcacgc ccaaaccgtc gaggttgatc gggttcgcga caccacctac cgccaggttc
     5701 tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc acgctggcac
     5761 tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg cagtttcatc
     5821 gccgggtgac ccccgtagcc attggcgaaa atacccaact gaccgctggc aaccagatct
     5881 ttgatcttgt tctgaacttt agtgaactct tccggactgt tcagatgcca ggtcgaaacg
     5941 cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt gatgtccacc
     6001 cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt gtgcgcagcc
     6061 agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag cgcactttct
     6121 gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat acgttgcaca
     6181 atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc gcgccacatg
     6241 gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc gatgcgtaaa
     6301 tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct ttgctcgcct
     6361 catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga cgaatgagta
     6421 cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt tcttcccagg
     6481 tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac cagcgaatag gtcagacgcc
     6541 aggttgcaca acctaacagt gcgctcagtg ctgacaggaa cagcatgcgg gaatcattac
     6601 gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact tcaatccaga
     6661 acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga tagataagct
     6721 cgccaaagcg cagcacgatg aaaatcgcca gcaacacact gatggtgttg gtcagcttga
     6781 cgaacagact cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc agcgaacctt
     6841 caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac agcggcaaca
     6901 tttcatagct ctgccacaac ggatgcacct tgtagcccgc cgagatcatc agtgacccca
     6961 ttgaagactg gtgcatggtc ggcaacagcg cgccaagtgc aatgatgaag aacataacct
     7021 tgttcagacg cttgagagaa accttccagc ccagacgttc aaacagtgcc ggagcaaact
     7081 ccagtgccat cacgccgata tagatggtca tacagaccgc cgtctcgaac agtaccgagt
     7141 tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga cccacgtcga
     7201 tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc gccggacgca
     7261 ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgccagc gcccagccgc
     7321 cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc cacgggaagc
     7381 cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca atcaggagca
     7441 tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt ttgccgccca
     7501 gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt tttggtgttg
     7561 cgacgaacca gcacggttaa gcccgccagc acagccagtg gtagcatcat gcctttatac
     7621 agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag tttcggcagg
     7681 tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc gccctctttc
     7741 tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc gccagatttc
     7801 agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg ttttttcgcc
     7861 tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc ccgccgggca cacttctacg
     7921 cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca cagctcgcac
     7981 ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta cggacaggcg
     8041 accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat gccggttttc
     8101 ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc gacgcagtgc
     8161 atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt gacccctgtg
     8221 ccgctggtcc acacctgaat gatgttattg gtatacggcg acagtttgtc gttgttcgac
     8281 caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca cttggtgacg
     8341 caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga tcccggaatt
     8401 ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt cagcaatgcc
     8461 ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc cgtgagtcag
     8521 cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg acaccggcaa
     8581 ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg ccgccctctt
     8641 tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg gcaagctgat
     8701 ggatgccttt atggaagccg ataccttctt cgttacagcc atagcatggg tgaccaatcg
     8761 ctaccggcca cacgccgcca acatcgcaga attgcagcgt tgagcagttg ccgtaagttt
     8821 ctggcccttt acagccgagg tggtacaggc accagccttc acggtggcct tcatcaccga
     8881 actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt tcgtgaatca
     8941 gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt ttgccgtaag
     9001 tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag cccggaatat
     9061 tgataacggt tttgcctggc agaacttctt gcaggctgac tgcgccagtt gggttaactc
     9121 cagctgcggc aacaccgccc cacgcagagc aggaaccgat agcgataatc gctgctgcgc
     9181 cttctgccgc tttgcggatg tgatccacaa tcggctcacc agcaaccatg caataaatac
     9241 cgttatcttt taatgggatg gaaccatcca caaccaacac atactgccct ttgtacttct
     9301 caagtgcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa agcacttcgt
     9361 gatactccag agagatagtc tccagcacga ggttttctac cgttggatgc gttgcacgaa
     9421 gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact ggcggacgct
     9481 gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac cccatggtgg
     9541 cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga gaatggatga
     9601 gggtgttatc tccagtcatt tatagttatt ccgttgcgaa gacctggcat atattttgcc
     9661 tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta ttatggttat
     9721 cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta gtgtaattaa
     9781 ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg ccataaaaaa
     9841 gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct ctttatccag
     9901 tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat ctccggtctt
     9961 ttcgtacatc cggtgtgagt agcctaatac aaaaccgcgt ttataatcgg cacagaattt
    10021 tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta agcctttacc
    10081 aaaatggtta ttcatcgttc acctcattcg ctatatatcg ccatatatag cgatatttta
    10141 ggcaaagaac gacttttgcg cacgctattt tttcatgctt gtttaagacg taatccagta
    10201 agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt tgccaggcaa
    10261 gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg tgattaaccc
    10321 ccggatagat atacgcctca taaaccttat tattggcttt caacgccgcc tcgtaagcag
    10381 gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa ggcgcttcaa
    10441 tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcaccgcg caggccagtt
    10501 ccggatacgc gacagccgcc gcgttcgata cgccaccgcc atagcaaaat ccggtaatac
    10561 ccactttgcc tgccgcttgt ggatagcgtt gcataaactc aattgcggca aagaaatcat
    10621 tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca tcatttcccg
    10681 gataacctcc aacggaactt aagccgtcag gtgccagggc gatatacccc gccttcgcca
    10741 ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc accaccacca
    10801 cggctggcgt tttgccgctc atttttgcgg gtttcaccag ataaccccgt acctcgccgt
    10861 gaccatttgg cgaaggatac atgatgtact cagcaacaat ctccgggtcg gtaaactcta
    10921 cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat gccgtcatcc
    10981 cgcccaccgc atacttcgcc gcaaggttga ggaactcacg tttcgaaatt ttcccgtgag
    11041 cgtaatagtc gtagtaatcc aacaactctt gtgggaaatc tttggcggtt aaacgcggca
    11101 tcgttgcact cctcagttgg tgttttttta agcaaagcat aagcacgtgt ttttgcccag
    11161 tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc taaactagaa
    11221 acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag cgaatcccga
    11281 acgttacggg cagatgcaat accgctattg cggaaaaagc ggtttacgcc tgcccgcgtt
    11341 atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac agcgtgcgat
    11401 cctgcgtaaa gcgtttgatt tgggcattac gcactttgat ttagccaaca attacgggcc
    11461 gcctccagga agcgcagaag agaactttgg tcgcctgctg cgggaggatt ttgccgctta
    11521 tcgcgatgaa ctgattatct ctaccaaggc tggctacgat atgtggcccg gcccttacgg
    11581 ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga agcgtatggg
    11641 gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc cgatggaaga
    11701 aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg tcgggatctc
    11761 ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt ggaaaattcc
    11821 gcttttaatt catcaacctt cgtacaattt actgaaccgc tgggtggata aaagcggcct
    11881 gctggatacc ctgcaaaata acggcgtggg ctgtattgcc tttactcctc tggctcaggg
    11941 attgctgacc ggaaaatatc tcaacggcat tccgcaagat tcacggatgc atcgtgaagg
    12001 gaataaagtt cgtggtctga cgccgaaaat gcttaccgaa gccaacctca acagcctgcg
    12061 cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg
    12121 gttgctgaaa gatgatcgcg tgacgtcggt attgattggt gccagccgcg cggagcaact
    12181 agaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc tggcgcagat
    12241 tgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcgtccg ataaatgacc
    12301 tgttaataac gggcgcgcga accgcgcccg aatcagatca gtgattatga cgagtcagtc
    12361 ggtcaagata gcccatcaca aatgcagaaa gcacaaacgt cagatggata atgacgtacc
    12421 acatcagttt gttatccggg acatttttcg catccataaa gacgcgcagt aagtgaatgg
    12481 aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgcg tccattttcc
    12541 ccagccagtt cagcttctct ttgttctcgg agatatccag ctgtgagacg aaattctcat
    12601 aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc accagcgaca
    12661 gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt acgtgaataa
    12721 tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca aagtacacgg
    12781 gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc atacattcct
    12841 gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcac gcaacagcaa
    12901 atcaacatat acgttaaacc tttgtcgcat aattctgagg tttagggatc ttcctggttc
    12961 ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa gggagaaaaa
    13021 cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat cccaaacaga
    13081 aacaaccgac gccaggcatc caggcgaaga tgacaccggt accggattgc ggcgagaaaa
    13141 cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg ggcgattccg
    13201 gaataggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg gcgatcagtt
    13261 atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa gaatgcggac
    13321 gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt tcgctggttc
    13381 acgaagcgca caaggcgtta ggcgggctgg atattatggc gctggtcgcc gggaaacagg
    13441 ttgccattcc ggatattgca gacctcacca gcgaacagtt tcaaaagacc tttgccatta
    13501 acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg aaaggtgcaa
    13561 gtatcatcac cacttcgtca atccaggcat accagccaag tccgcattta ctggactatg
    13621 cggctacgaa ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag gtcgcggaga
    13681 aaggtattcg ggtgaatatt gtcgcgccag gccctatctg gacggcactg caaatttccg
    13741 gcggacaaac gcaggataag atcccgcagt ttggtcagaa aacgccgatg aagcgtgcgg
    13801 ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag tcgagctacg
    13861 tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa aatgcccggt
    13921 tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc gaaaacgcct
    13981 tatccggcct acaaacgcac gaagattcaa catgttgcaa tagattttgt aggcctgatg
    14041 agcgtagcgc atcaggcaat tctactttac ttcgctttgg cggtttcttc gccgaccaga
    14101 cctatcttca ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa cgtctcgtaa
    14161 tcgacggttt tgtccgctcg gaagaagatg gtggtgtctt tcttgccttc ggttaacgca
    14221 ttcaacgccg taatcattgt ttcatcggtg accggatcgt taccgataaa catcgagttg
    14281 tctgccttca ccgacagata aaccggtttt tccgggcgcg gctgcggcgt gctggtagaa
    14341 gcaggcaagt tcaccttcac atctaccgtc gctaacggtg ccgccaccat aaagataatc
    14401 agcagaacca acatcacgtc gataaacggc gtcacgttga tatcatgcat ttcgccgtta
    14461 tcgtcgaggt tttcgttaag atgcattgcc atcggacatt atcctgcgcg taatttttgt
    14521 gcgacacgaa ccggatgcgc agcggcgctg gcttccagat ccaggtcacg gctttgcagc
    14581 aacaatacct gcgctgcaac atcacccagc atcgctttaa agccgccaat ctggcgtgca
    14641 aatacgttat agataacgac cgcaggaatc gctgccacga ggccgattgc cgttgctaac
    14701 agagcttctg cgatacccgg cgcaacgact gccaggttag tggtctgcgt ttgcgcgata
    14761 ccaataaagc tgttcatgat gccccagacc gtaccaaaca gaccaacgaa cggagaaatc
    14821 gcgccgatgg ttgccagata gccgttaccg cggcccattt gacgacccac tgcggcgacc
    14881 cgacgctcca gacggaagct ggtacgttct ttaatacctt cgttatcgtc gctgccttct
    14941 gacagttcca gctcattctg cgcttcattg agcaaatgca ggcttaagct tttgctacca
    15001 aaatcagcgg cgatatcgtt ggcctggttt aaggaacgcg cttcagccag cagttgctgc
    15061 tcgcgcttaa ggcgacgctt ctgattgaag aactctacgc tcttactgaa gaagattgcc
    15121 caagtgacta cggaggccaa aataagccca atcatcacgc acttaacgac aatatcggcg
    15181 tgctgataca taccccagac ggaaaggtcc gtctgcatca aattattacc cactgtgtat
    15241 ctccaggacg caagtcacaa aatctgcgca taataatatc aaaatgacgt cgaattgata
    15301 gtcgttctca ttactatttg catactgccg tacctttgct ttcttttcct tgcgtttacg
    15361 cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc tgctttatgc caattcttca
    15421 ggatgcgccc gcgaatattc atgctagttt agacatccag acgtataaaa acaggaatcc
    15481 cgagatggcg gacaaaaagc ttgatactca actggtgaat gcaggacgca gcaaaaaata
    15541 cactctcggc gcggtaaata gcgtgattca gcgcgcttct tcgctggtct ttgacagtgt
    15601 ggaagccaaa aagcacgcga cgcgcaatcg cgccaatggc gagttgttct atggacggcg
    15661 cggaacgtta acccatttct ccttacaaca agcgatgtgt gaactggaag gtggcgcagg
    15721 ctgcgtgcta tttccctgcg gggcggcagc ggttgctaat tccattcttg cttttgtcga
    15781 acagggcgat catgtgttga tgaccaacac cgcctatgaa ccgagtcagg atttctgtag
    15841 caaaatcctc agcaaactgg gcgtaacgac atcgtggttt gatccgctga ttggtgccga
    15901 tatcgttaag catctgcagc caaacactaa aatcgtgttt ctggaatcgc caggctccat
    15961 caccatggaa gtccacgacg ttccggcgat tgttgccgcc gtacgcagtg tggtgccgga
    16021 tgccatcatt atgatcgaca acacctgggc agccggtgtg ctgtttaagg cgctggattt
    16081 tggcatcgat gtttctattc aagccgccac caaatatctg gttgggcatt cagatgcgat
    16141 gattggcact gccgtgtgca atgcccgttg ctgggagcag ctacgggaaa acgcctatct
    16201 gatgggccag atggtcgatg ccgataccgc ctatataacc agccgtggcc tgcgcacatt
    16261 aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa gtggctgaat ggctggcaga
    16321 acatccgcaa gtagcgcgag ttaaccaccc tgctctgcct ggcagtaaag gccacgaatt
    16381 ctggaaacga gactttacag gcagcagcgg gctattttcc tttgtgctta agaaaaaact
    16441 cagtaatgaa gagctggcga actatctgga taacttcagt ttattcagca tggcctactc
    16501 gtggggcggg tatgaatcgt tgatcctggc gaatcaacca gaacatatcg ccgccattcg
    16561 cccacaaggc gagatcgatt ttagcgggac cttgattcgc ctgcatattg gtctggaaga
    16621 tgtcgacgat ctgattgccg atctggacgc cggttttgcg cgaattgtat aacattgcca
    16681 cttttggaca attttgcaga catttaattg tgaaaagtct taaattgttg cgtccgggat
    16741 caaggcgtcc cggacgaatc aggagtacaa taggcagata aaggcttaaa cgctgttcca
    16801 caggaaagtc catggctgtt attcaagata tcatcgctgc gctctggcaa cacgactttg
    16861 ccgcgctggc ggatcctcat attgttagcg ttgtttactt tgtcatgttt gccacgctgt
    16921 ttttagaaaa cggcctgctg cccgcctcat ttttgccagg cgacagcttg ttgatactgg
    16981 caggcgcatt gattgcccaa ggggttatgg attttctgcc tacgattgcg attctgaccg
    17041 ccgcagcaag tctgggctgc tggctaagtt atattcaggg gcgctggtta gggaatacca
    17101 aaacggtgaa aggctggctg gcacagcttc ctgctaaata tcaccagcgc gccacctgca
    17161 tgtttgaccg ccacggtctg ctggcgctgc tggctggacg ttttcttgca tttgtccgta
    17221 cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc cagtttttca
    17281 actggttaag tggattgctg tgggtcagcg tggtaaccag ttttggctat gccttaagta
    17341 tgattccgtt cgttaaacgc catgaagatc aggtaatgac gttcctgatg atcctgccaa
    17401 ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt agtgattaaa aaaaaatact
    17461 gtaacgcctg acgattttcc ccgttcccgg ctgctgtacc gggaacgtat ttaattcccc
    17521 tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca caccgaagta acgtttaaac
    17581 tcacggctaa attgcaatgc gctttcatag tcgacgcgca tcgctgctgc gctggccttc
    17641 atgccgtcat ggatgatcat catccgcgcc ttatgcagac ggtaattctt caaatactgc
    17701 aacggcgagg tgctggtgac agacttaaaa ttatgatgga acgccgatac gctcatgttg
    17761 gcttctgccg ccagttgctc gacgctcagg ttttcggtgt atttattctc aatccgtttc
    17821 agcacgcggc taatcagact gaagtgagtc tggcgactga ccagcgccag taacgcgccg
    17881 ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga tgatctgttt gccgagaata
    17941 cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca taaaatctct
    18001 tctgataacg tggcggagtt aatcccgctg gctgccatcg acggctggaa atgctcatct
    18061 tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat cgacattgag acgcaatcct
    18121 gccagcggca cctctgacgt ggcataggtt tcgcactcaa acggcaacgg caccgtcagc
    18181 agcaggtatt cattggcatc ataacgaaac acgcgttcat tgatataacc gattttatgc
    18241 ccggaaaaga gaattatgat gccaggctcg tacatcaccg gtgtacgtgc gaaaggcgtc
    18301 tcgccataca acaaacgcac atcgggcaac agttctgaca aactattttc tttatttttc
    18361 agtttattaa ctttatccgc cagcaagcgg caaatctctt cacgtttcat atcgcgtaat
    18421 ttcttaggaa taatgcggca atttgattgt gcgcaatttt gtagcatttc tccagcactc
    18481 tggagaaata ggcaagacat tggcagaaat gagcattgag agccagggcg ctggcgatca
    18541 caatgaaaaa catcaggcag atcgttctct gccctcatat tggcccagca aagggagcaa
    18601 gtaatgaaca actttaatct gcacacccca acccgcattc tgtttggtaa aggcgcaatc
    18661 gctggtttac gcgaacaaat tcctcacgat gctcgcgtat tgattaccta cggcggcggc
    18721 agcgtgaaaa aaatcggcgt tctcgatcaa gttctggatg ccctgaaagg catggacgtg
    18781 ctggaatttg gcggtattga gccaaacccg gcttatgaaa cgctgatgaa cgccgtgaaa
    18841 ctggttcgcg aacagaaagt gactttcctg ctggcggttg gcggcggttc tgtactggac
    18901 ggcaccaaat ttatcgccgc agcggctaac tatccggaaa atatcgatcc gtggcacatt
    18961 ctgcaaacgg gcggtaaaga gattaaaagc gccatcccga tgggctgtgt gctgacgctg
    19021 ccagcaaccg gttcagaatc caacgcaggc gcggtgatct cccgtaaaac cacaggcgac
    19081 aagcaggcgt tccattctgc ccatgttcag ccggtatttg ccgtgctcga tccggtttat
    19141 acctacaccc tgccgccgcg tcaggtggct aacggcgtag tggacgcctt tgtacacacc
    19201 gtggaacagt atgttaccaa accggttgat gccaaaattc aggaccgttt cgcagaaggc
    19261 attttgctga cgctaatcga agatggtccg aaagccctga aagagccaga aaactacgat
    19321 gtgcgcgcca acgtcatgtg ggcggcgact caggcgctga acggtttgat tggcgctggc
    19381 gtaccgcagg actgggcaac gcatatgctg ggccacgaac tgactgcgat gcacggtctg
    19441 gatcacgcgc aaacactggc tatcgtcctg cctgcactgt ggaatgaaaa acgcgagacc
    19501 aagcgcgcta agctgctgca atatgctgaa cgcgtctgga acatcactga aggttccgac
    19561 gatgagcgta ttgacgccgc gattgccgca acccgcaatt tctttgagca attaggcgtg
    19621 ccgacccacc tctccgacta cggtctggac ggcagctcca tcccggcttt gctgaaaaaa
    19681 ctggaagagc acggcatgac ccaactgggc gaaaatcatg acattacgtt ggatgtcagc
    19741 cgccgtatat acgaagccgc ccgctaagct ttttacgcct caaactttcg ttttcgggca
    19801 tttcgtccag acttaagttc acaacacctc accggagcct gctccggtga gttcatataa
    19861 aggaggaacg tatggctaat ccaaccgtta ttaagctaca ggatggcaat gtcatgcccc
    19921 agctgggact gggcgtctgg caagcaagta atgaggaagt aatcaccgcc attcaaaaag
    19981 cgttagaagt gggttatcgc tcgattgata ccgccgcggc ctacaagaac gaagaaggtg
    20041 tcggcaaagc cctgaaaaat gcctcagtca acagagaaga actgttcatc accactaagc
    20101 tgtggaacga cgaccacaag cgcccccgcg aagccctgct cgacagcctg aaaaaactcc
    20161 agcttgatta tatcgacctc tacttaatgc actggcccgt tcccgctatc gaccattatg
    20221 tcgaagcatg gaaaggcatg atcgaattgc aaaaagaggg attaatcaaa agcatcggcg
    20281 tgtgcaactt ccagatccat cacctgcaac gcctgattga tgaaactggc gtgacgcctg
    20341 tgataaacca gatcgaactt catccgctga tgcaacaacg ccagctacac gcctggaacg
    20401 cgacacacaa aatccagacc gaatcctgga gcccattagc gcaaggaggg aaaggcgttt
    20461 tcgatcagaa agtcattcgc gatctggcag ataaatacgg caaagccccg gcgcagattg
    20521 ttatccgctg gcatctggat agcggcctgg tggtgatccc gaaatcggtc acaccttcac
    20581 gtattgccga aaactttgat gtctgggatt tccgtctcga caaagacgaa ctcggcgaaa
    20641 ttgcaaaact cgatcagggc aagcgtctcg gtcccgatcc tgaccagttc ggcggctaac
    20701 atgcaaattc tcccggtgac ggtaatgttc cgctaccgga cttttcagaa atcatttatt
    20761 cccctcgcgt cccgcccgtt gttactcttc cttgttcagg aatgccaaat ataaggacat
    20821 catcatgc
//
LOCUS       DAZOZY010000056        20812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZOZY010000056 DAZOZY010000000
VERSION     DAZOZY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.056"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHURQ_001820"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHURQ_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR0823615.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="ACHURQ_001821"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="ACHURQ_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHURQ_001822"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHURQ_001822"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR0823616.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHURQ_001823"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHURQ_001823"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR0823617.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHURQ_001824"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHURQ_001824"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR0823618.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHURQ_001825"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHURQ_001825"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR0823619.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHURQ_001826"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHURQ_001826"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR0823620.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="ACHURQ_001827"
     CDS             8330..10048
                     /locus_tag="ACHURQ_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR0823621.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHURQ_001828"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHURQ_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR0823622.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHURQ_001829"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHURQ_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR0823623.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="ACHURQ_001830"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="ACHURQ_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR0823624.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHURQ_001831"
     CDS             complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHURQ_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR0823625.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHURQ_001832"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHURQ_001832"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR0823626.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHURQ_001833"
                     /pseudo
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHURQ_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHURQ_001834"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHURQ_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR0823627.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHURQ_001835"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHURQ_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR0823628.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="ACHURQ_001836"
     CDS             complement(20443..20718)
                     /locus_tag="ACHURQ_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR0823629.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4688 a         5515 c         5697 g         4912 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggtcagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaactg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggt ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctacgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aa
//
LOCUS       DAZOZY010000057        20517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZOZY010000057 DAZOZY010000000
VERSION     DAZOZY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.057"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..606
                     /locus_tag="ACHURQ_001837"
     CDS             <1..606
                     /locus_tag="ACHURQ_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0823630.1"
                     /translation="GIKEFDLSSSWGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQR
                     SSPEEWKALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLT
                     EEESLWLQSRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDS
                     AGSRMFEELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
     gene            603..2054
                     /gene="djlC"
                     /locus_tag="ACHURQ_001838"
     CDS             603..2054
                     /gene="djlC"
                     /locus_tag="ACHURQ_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR0823631.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(2114..3784)
                     /gene="hscC"
                     /locus_tag="ACHURQ_001839"
     CDS             complement(2114..3784)
                     /gene="hscC"
                     /locus_tag="ACHURQ_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR0823632.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(3900..4835)
                     /gene="rihA"
                     /locus_tag="ACHURQ_001840"
     CDS             complement(3900..4835)
                     /gene="rihA"
                     /locus_tag="ACHURQ_001840"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR0823633.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(4953..5678)
                     /gene="gltL"
                     /locus_tag="ACHURQ_001841"
     CDS             complement(4953..5678)
                     /gene="gltL"
                     /locus_tag="ACHURQ_001841"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR0823634.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5678..6352)
                     /gene="gltK"
                     /locus_tag="ACHURQ_001842"
     CDS             complement(5678..6352)
                     /gene="gltK"
                     /locus_tag="ACHURQ_001842"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR0823635.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6352..7092)
                     /gene="gltJ"
                     /locus_tag="ACHURQ_001843"
     CDS             complement(6352..7092)
                     /gene="gltJ"
                     /locus_tag="ACHURQ_001843"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR0823636.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7262..8170)
                     /gene="gltI"
                     /locus_tag="ACHURQ_001844"
     CDS             complement(7262..8170)
                     /gene="gltI"
                     /locus_tag="ACHURQ_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR0823637.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8625..10502
                     /locus_tag="ACHURQ_001845"
     CDS             8625..10502
                     /locus_tag="ACHURQ_001845"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR0823638.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10580..12118)
                     /gene="lnt"
                     /locus_tag="ACHURQ_001846"
     CDS             complement(10580..12118)
                     /gene="lnt"
                     /locus_tag="ACHURQ_001846"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR0823639.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12143..13021)
                     /gene="corC"
                     /locus_tag="ACHURQ_001847"
     CDS             complement(12143..13021)
                     /gene="corC"
                     /locus_tag="ACHURQ_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR0823640.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13111..13578)
                     /gene="ybeY"
                     /locus_tag="ACHURQ_001848"
     CDS             complement(13111..13578)
                     /gene="ybeY"
                     /locus_tag="ACHURQ_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR0823641.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13575..14615)
                     /locus_tag="ACHURQ_001849"
     CDS             complement(13575..14615)
                     /locus_tag="ACHURQ_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR0823642.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14768..16192)
                     /gene="miaB"
                     /locus_tag="ACHURQ_001850"
     CDS             complement(14768..16192)
                     /gene="miaB"
                     /locus_tag="ACHURQ_001850"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR0823643.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16338..17513
                     /gene="ubiF"
                     /locus_tag="ACHURQ_001851"
     CDS             16338..17513
                     /gene="ubiF"
                     /locus_tag="ACHURQ_001851"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR0823644.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17667..17741)
                     /locus_tag="ACHURQ_001852"
     tRNA            complement(17667..17741)
                     /locus_tag="ACHURQ_001852"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17707..17709),aa:Gln,seq:ctg)
     gene            complement(17790..17866)
                     /locus_tag="ACHURQ_001853"
     tRNA            complement(17790..17866)
                     /locus_tag="ACHURQ_001853"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17830..17832),aa:Met,seq:cat)
     gene            complement(17882..17956)
                     /locus_tag="ACHURQ_001854"
     tRNA            complement(17882..17956)
                     /locus_tag="ACHURQ_001854"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17922..17924),aa:Gln,seq:ttg)
     gene            complement(17991..18065)
                     /locus_tag="ACHURQ_001855"
     tRNA            complement(17991..18065)
                     /locus_tag="ACHURQ_001855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18031..18033),aa:Gln,seq:ttg)
     gene            complement(18089..18173)
                     /locus_tag="ACHURQ_001856"
     tRNA            complement(18089..18173)
                     /locus_tag="ACHURQ_001856"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18137..18139),aa:Leu,seq:tag)
     gene            complement(18182..18258)
                     /locus_tag="ACHURQ_001857"
     tRNA            complement(18182..18258)
                     /locus_tag="ACHURQ_001857"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18222..18224),aa:Met,seq:cat)
     gene            complement(18638..20302)
                     /gene="asnB"
                     /locus_tag="ACHURQ_001858"
     CDS             complement(18638..20302)
                     /gene="asnB"
                     /locus_tag="ACHURQ_001858"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR0823645.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(20256..>20517)
                     /locus_tag="ACHURQ_001859"
     CDS             complement(20256..>20517)
                     /locus_tag="ACHURQ_001859"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823646.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT         4987 a         5156 c         5325 g         5049 t
ORIGIN      
        1 gggataaaag aattcgatct ctcatcatcc tggggaataa ataatcgtga tgatttaatt
       61 cagactattt atcagatgac tgatgatggt catgccaatg atttagctgg cttatatctg
      121 acatggcaac gctcttcccc tgaagagtgg aaagcattaa ttgctggtgg ctcggagaga
      181 gggttgattt atacccaatt cgttgcccaa accgctatgt gctgtggaga agggggaatc
      241 aaagcctggg attatgttcg aatggggttt ctgtcacggg tcggtgtgct caacaactgg
      301 ctgacggaag aagagagttt atggctgcaa tcgcgcgtct atgcacgagc tcaccattac
      361 tatcacagct ggatgcacta tttttctgcc tattcactag ggcgtctcta ctggcaatct
      421 tctcagtgcg aagacaacgc ttcgctacgc gaggcgctaa ccctgtacaa atacgatagc
      481 gccgggagtc ggatgttcga agagctggca gcgggaagcg accgtttcta tgccacgctt
      541 ccctggcaac cattaactgt ccagcctgaa tgcccggtaa cgcttaagga tgtgagcgac
      601 ctatgaaaac atgttggcaa atcctcgaaa ttgaaagcac gacgcaaata gacattatcc
      661 gccaggctta tcttgctcgc ttaccgttgt gtcatcccga aaccgatccg caggggttta
      721 aagcattacg ccaggcctat gaagaggcgc tgcgactggc ggtaaatcct gtcgaggaag
      781 cagatgatga agaaaaagat gccaccgctg aacatgaaat actacgtgca ttcaggacat
      841 tactggattc agaaagtgat cgttttcagc cctccgcctg gcagaaattt attcagcaat
      901 taaatacctg gaacatggag gatgtcgatc aattacgctg gccgctgtgt gcaatcgcta
      961 tagaagcgcg atatctttca ttaaattgtg cttctttgtt ggcagagcgt ttgaactggc
     1021 attcgtttaa tgacagcgaa ggaatggatg aggaagaaag ggaggctttt cttgaggcca
     1081 ttcaggctgg tgattgtttc gatttcctta gccttctgga atatcccgtc gcgttacaga
     1141 accagactgt tgaatattac ttcgcgctgg aacgttgctg ccgttaccat cctgactatg
     1201 tcactgcgtt tttggcgatg gaaggaccgt ggttcattcc tgatgatgca aagttacatc
     1261 gcaaactgtt gcgctggtac agctcggtgc aaacaggtat ggcggaactc attcctgtag
     1321 ccaaacagtg gcaagcggaa gaaccagaaa gcgaagatgc ccggtattac cagtgtgcac
     1381 aacgtttgta ctgcggcgag ggggaaagcc tgcttgccga tctctgcgcg tactgggaaa
     1441 gttacccatc tacacaagct gataatttgt tgttgcagtg gagcaagcgt cattgcccgg
     1501 attatttcgc gttattagtg atggttatcg aagcgcgaag catggtagat gcgcaaggta
     1561 aaccgttgaa atatgttcct ggtgagagcg cccggacgcg gctgttatgg gcggagattt
     1621 tacatagcgg aaaattatcg ccgttaggtc aatcgtttat tgagtcgtta ttcttcaagc
     1681 gcaaagcatg ggcgtggtgg aaatcgagag tcggtagcga gacagagcaa gattcaccgt
     1741 tactggattt gtatcgggta gcggaacagg ttgtgctgga agcgttcccg aaacaagaga
     1801 tgctggcccg tcttaataca aggctggaag gtggagatgc tcatccatta gaggccatta
     1861 tcacccagat gctgttgacg aaagtgaaac tcgagccgga ggatgaagat gtcgatgagc
     1921 caacacctga aaaccatgaa gaaaaaaatg atgaggatga aaaaccacag agcattacca
     1981 gtattatcaa aatcagctta acggtgctga tgataggtta tgcgctcgga aaaatcgcga
     2041 tgttgtttag ctgaggatgt gttttttgcg ccgatgccgg atgcagcgtt tatgccgcat
     2101 ccggcagccg tgtttaaggg atctcaatag ccaaataatc gctcgcccgt cgacgaactt
     2161 catcgacgcg ggccatatcg tttgactgca acgccgcatc aaaatcggtg atcacccggc
     2221 caatctcttc tcgctcgtca cccagcgccc gcgcccacaa ctcttccagt ttggccttaa
     2281 aggtgcggtt gattagcata tcgcgcggat aaattttcaa tgcggataac cgcgtccgac
     2341 tctcttcaat ctgctgcgcg ctcaatgtta ccgggctatg attaatgact ctggacttaa
     2401 cgctgccatc ttccagaagc acgtcaactt caagcaagcc gttgatatca taactaaagc
     2461 gaatatcaat cgactgataa gccccggttt tcttcaacgg cacatcgaag gattcaacca
     2521 gaatgttatt tttaactttg tggttttctc cctgatagac gttaaccgta atagaatcct
     2581 gttccgggtg catggtcgag taagtttcta cacgcgacac gggtactgtg gtgtttcgtt
     2641 caataattgg cgagaaaatg ccggaaacgc cctggcggtt aacttcaacg cccaacgagt
     2701 aagggcaaat atcagtgagg atcacctctt caatatcttc actgcgtaag cggcaggcgg
     2761 cctggattgc tgcgcccagc gcgacaatgg tgctcgggtc gtaactttga tacggtaatt
     2821 tgccaaacag acgcacggcg attcgctgca ccagcggcat ttgtgacgcg ccgccaacca
     2881 gcaccagact gtcgatttga ctcggcttca gacgtgcatc gcgcaacgcc tgttcaatcg
     2941 gcactcgcaa gcgattgagc agcggcaacc acaaatcttc cagttcgttc tcgtaaaatt
     3001 cgcactcccg cgtttcatcc tggtactgcc agcgaatgtg caatggcgat tgattgctac
     3061 attttgccgc ttccacacag gcgtacaagg ctgccagttc actctcgtta agcgtggtcc
     3121 tggcgacatc cgcgcgcttt aaaacgtcat cgaccaacat atgggtaaaa tcttcgccac
     3181 cgaggaagtt atcgccagcg gaggcgtgaa cttcaatcac cggcgtggcg tattcgagca
     3241 ccgtgacgtc aaacgtgccg ccaccgagat caaaaaccag cgagcgggta ttttgttgcg
     3301 tatgcaggcc atatgccatc gcagctgctg tgggttcatt aattaagcgt accgcattta
     3361 acccggctaa ctccgctgct aaacgggtat gcttgcgttg ttcatcgctg aaataagccg
     3421 gaacggagat caccacatct ttaatcggac gttgcagaaa ttcttcggca tcttctttta
     3481 atgagcgtaa taccaaagag gacagttctg gcgcgttaaa tgtgtcgctg cctaaccgcc
     3541 agttggtatt actgcccatt gcacgtttaa ataacgctgc cgttttatcc ggatgcgaag
     3601 tacgccgtga tacagccggt tttccgacta aaatatgatt attttcatcc atgctaatta
     3661 tggatggtgt taaatattca ccgaacttat ttggaattaa ttgcgcggca ccgtctttcc
     3721 agacggcaat taaactattg gtagtgccga gatcgatacc aatggcgagt tctgcattat
     3781 ccattgcatt tatatcttga aagaaaaaga gataaacaga taaacagata aacagataaa
     3841 cagataaaca gattaagacc acccaggtaa cgcgtggtga tcttaatcaa tgacgtgtgt
     3901 taagcgtaaa atttcagacg atcagccagt aaatcaacaa agccctgacg atcaacatcg
     3961 accattacag tggcattcgg tttattgcca gtcagataat aataatcaac aaccgtcata
     4021 ccctggctat atttcccctg tgtttccacg ccaacccagc gctcaacagt ggtaaacaac
     4081 tcgggtttca acagccaggc gatggtgcat gggtcatgca gcggtgcacc gacaaagccc
     4141 catttttcgt ctttatgata ttcgaggaag aaatccagca gttcggcaac aatggttgaa
     4201 acagggttgc caatcgcgcg gaaacgctcg gtgtcttcaa cgtggatttg tgctttgtga
     4261 gtaacatcca gaccggccat caccactggg atccctgact ggaagacaat ttctgccgct
     4321 tccgggtcaa cgtaaatatt aaattcagcc gcaggcgtcc agttaccaag ccccattgcg
     4381 ccgcccataa tcacgatacg ggcaattttg ctatgcagtt ccggatggct attgagcagc
     4441 aaggcaacgt tagtttgcgg tccggtagac acaatcgtga caggttccgc actttcgcgc
     4501 agcgttttcg ccatcagctc taccgccgta cagttttgcg gtgcgaacgt cggttccggt
     4561 aatgccgggc catccagacc gctttcgccg tgcacattgt ccgcgataat caactcacgc
     4621 attaacggtt ttaccgcgcc gcctgctacc ggaatatcgg tgcgattaag caaggtcagc
     4681 atacgcagaa cattgcgtaa ggttttttct ggtgtctggt ttccggcgga agacgtaatt
     4741 gctttgacat caagctctgg tgaggcgagg gcgagaacta ttgcgatagc gtcgtcatga
     4801 cctgggtcgc aatctaacag aattggcagt gccattgttg ctccttgttg tgtgcttctt
     4861 tgcgacaagg gtaacgccag gatgtaacag atacgagggg cgaaacgata aagcgtgaga
     4921 tggcgcgcac ttgggtatgc gcgccagagt gattaatgca ggattttcgc gaggaagtct
     4981 ttcgcgcggt ccgatttcgg atcatcgaag aaagcgtctt tcggcgagtc ttcgacaatt
     5041 ttaccctcgt ccataaagat cacccgattc gccactttgc gggcaaagcc catttcgtgg
     5101 gtcaccacca tcatggtcat tccttcgttc gccagttcca ccatcacgtc cagcacttcg
     5161 ttgatcatct ccggatccag cgccgatgtc ggttcgtcga acagcatcgc aataggatcc
     5221 atacacaatg cgcgagcgat tgccacacgc tgctgctgac cgccggaaag ctgcgccgga
     5281 aatttattgg catgtgcgga aagtcccacg cgctccagca atttcaatgc tttttcacga
     5341 gcgggcgctt tatcgcgttt aagcactttc acctgcgcca gggtcaggtt ttcgataatc
     5401 gacagatgag ggaacagctc gaaatgctgg aataccatcc cgacgcggga acgcagcttt
     5461 gccagatcgg ttttcttgtc attaaccacg ataccatcga cggtgatttc accttgctgc
     5521 actggttcga ggccgttgac ggttttaatc agcgttgatt tgccggaacc agacgggccg
     5581 caaaccacca ccacttcgcc ttttttcact tcggttgagc agtcggtcag cacctgaaag
     5641 tgaccatacc attttgaaac atttttcagg gtaatcatta tgctgtcctt cttttcaagt
     5701 agctgaccaa caacgacgcg ctaagactaa taacgaaata aacaaatccg gcaaacagga
     5761 tcatctcaac ctgcgtacca tcacgctcac caatggttga ggcggtacgg aagaaatcgg
     5821 ccagagataa cacatacacc agtgaggtat cctggaacag tacgatgccc tgagtgagca
     5881 gaagcggcac catcgcgcgg aacgcctgcg gcagaataat cagtttcatc gactgccagt
     5941 gagtcattcc caacgccagc gcggcgctcg actgaccacg agaaatactt tgaataccag
     6001 cacggataat ctctgaatag taggccgctt caaacatcga aaacgccacc atcgccgaaa
     6061 ttaaacggat atcatttttt ggtgataatc ccagcacgtt ttgcagaaaa cctggcacga
     6121 tcaggtaaaa ccacagcaaa accataacta aaggaatcga gcggaatacg ttaacgtagg
     6181 ctttggcaaa ccacgctacg ggcgcaaagc tggacaagcg catcaccgcc agcatggtgc
     6241 cccacaaaat accaatcacg actgccgtga cggtgatttt cagcgtgatc accagcccgt
     6301 cgagcagata tggcagggaa gggacaatgg aactccagtc aaactcgtac attatttgcc
     6361 ccccatgttg ccaggcaggc gaactttacg ttcaaccagc gtcattacca gcatgataac
     6421 cgcgttaatc aacacatacg ccagcgtaat agcggtaaac gactcccagg cgtgggctga
     6481 gtaatcaagc aatttacccg cctgcgccgc catatccacc agaccaatag tcgaggcgat
     6541 ggcggagttt ttcaccaggt tcatcatctc ggaagtcatc ggcgggacga taacgcgata
     6601 agcattaggc agcagtacgt atcgataagc ctgcggtagc gtcaggccca tcgccagcgc
     6661 ggcatttttt tgcccacgcg gcaacgactg aatggcggcg cgtacctgtt cgcaaacacg
     6721 ggcggcggta aacagcccca ggcagagcat ggatgaaagg aaaaactgaa tattgggatc
     6781 cagctcggcc ttaaaccaca tgccgatttt ctccggcaac agctccggga tcaccagata
     6841 ccaggtaaag aattgcacaa tcaacggcac gttgcggaac agttcgacat acaacgtacc
     6901 cagaccggag agaaaacggt ttggaacggt acgtaaaatg ccgaaaaatg aaccgacgag
     6961 gaaagcgata atccaggcgc agatcgacaa agcgatcgtc acctgaaaac cactccagat
     7021 ccaaccaaga taggtggtgt tgccgaacgg ggcttgttgt aaaaaaatac cccagttcca
     7081 gtctatagac ataaatctac tccagaaaaa agagggtagc agcgttaact gctaccctcg
     7141 aagattgtta cccagcgtat tgcggttttc aggcccgatg gggaacgacc atcaggcgta
     7201 tagtctgtcc gtgctacgta acaatcgaga gggctggaat ttccgcccct ggttcttgta
     7261 attagttcag tgccttgtca ttcggttctt tgaacagtgc tttcatttcg tctgacagtt
     7321 cgaaattcat gttcaggttt ttcggcggaa tgggattttt gaaccattta tcaaaccatt
     7381 tttccgcttc accggaggtc tgcacctgcg cggtggtgtc atccatcagc tttttgaact
     7441 gcggatcgtc tttacgcaac atgcaaccgt aggcttcctg agactgcggc ttgccgacga
     7501 tttcccagtt gtctggtttc ttcgctttcg cacgttcacc ggccagcagg gcatcatcca
     7561 tcataaaggc aacggcacga ccgctttcca gggtgcggaa agagtcactg tgatctttgg
     7621 cgctgatgat gcgcatattc attttttgct cttcattcag tttgttgagc aaaacttcag
     7681 aggtagtgcc ggaagtgacg actacggctt tgtctttcag gtcggcaaaa tctttgatat
     7741 cgccgccctt tttggtcagc aggcgcgtac cgaccacgaa aatagtatca gagaaagccg
     7801 cctgtttttg gcgttcgacg ttgttggtgg tagaaccaca ttcaaaatcg aaagtgccgt
     7861 tttgcagcag tggaatacgg ttttgtgagg taatcggaat cagttttacc tgcaagtccg
     7921 gtttgttgag tttctttttc actgcttcaa caatggcgtt ggagtaatcc tgcgagtaac
     7981 ccaccacttt ttgctgattg tcgtaataag agaaaggtac tgaagattca cggtgaccga
     8041 cgacaatcac accgtttttg gcgattttgt ccagcgtact gcctgctgcc ggggcggcgt
     8101 catctgcctg tgccagtcct gcggaaagcg ccagggcgag gattgctgtg gcaggtttac
     8161 gtaattgcat atccaactcc tttatcttct gcgttaagaa cgcatggata cccgttgtga
     8221 gtgtttgtgt tgttatcgtc tgcaacttta ttgtgcagtg ttgtgcctgt taggtaacat
     8281 ttagtttggc taaatgtaaa gatattgctg ttttattgtt tgtttttgcg agatgcgccg
     8341 caccattccg aagcaaaatt cttaaaccgc acctttttgg tgctaccgct gatttgtggt
     8401 ggtgcaacct ggttgcgtcg cggctgattt catcgcttac acagtaaata aagcaactta
     8461 catgccaaaa aatacttagg ggcgaattaa ttacgattgt aattttaggc tgaaaaagtc
     8521 atatttcgga cagaattatc ttttcggcat gggacgtttg ccgggatgat aactgcacac
     8581 gggaaaaatg ctttttacct gtcttttcaa ggaagaaaca ccttatgtct gcatcttcgg
     8641 ttaagccgtt aaatgttcaa ttacccgcaa taacccttat cctttttgca ctctgtattg
     8701 ggatattttg ttatctcgca caatggatga gttatgaaga ggtcgatcaa tccgcactta
     8761 tccatctcgg cgcaaacgtt gcgcctctta ctttgtcggg tgaaccctgg cgcttattga
     8821 gcagtatctt tctgcacagt agtgtttctc atctgctgat gaatatgttt gcattcctgg
     8881 tcgtgggagg cgtggcggaa cagattctgg ggaaatggcg actcctgatt acctggttat
     8941 tctccggcgt ctttggtggg ctcatcagcg cctgttatgc gttacgcgaa agtgagcaga
     9001 tagtcatcag cgttggggca tccggggcga ttctgggaat agctggtgca gcgatagcaa
     9061 cacagtttgc ttcaggtacg ggcacatacc ataaaaacca gcggcgagta tttcctctgt
     9121 tgggtatggt ggcgctgaca ctgttgtacg gtgcccggca aacaggaata gataacgctt
     9181 gccacattgg cggcctgatt gcgggtggcg cgttgggttg gctgagcgcg cgtttatctg
     9241 ggcaaaaccg actcgttacg gaaggcggga ttattgttgc gggcagtctt cttctgaccg
     9301 gggctatctg gcttgcgcag cagcagatgg atgagtcagt tttacaggtc aggcaaagcc
     9361 tgcgtgaagc gttttatcca caggagattg aacaagagcg acggcaaaaa aagcagcagt
     9421 tagcggagga acgcaacgcc ctcagggaaa cattatccgc tccggtaagt cgtgaacagg
     9481 ccagtggtga tttgctcgct gagattgccg atatccatga tatggcgatc agtcgggatg
     9541 gtaatatgtt gtatgccgca attgaaaaca cgaacagcat tgttgttttc gacctcggac
     9601 aaaagaaaat cctgcatacc tttacagcgc ccatagcgaa agaaaagtca gtcaaacatt
     9661 gtggtggctg taaagatcag ggcgtcagat cgctggcatt aagcccggat gaaaagttga
     9721 tttatgcgac ttcatttgaa gcgaatgcgt tatcggtcat taacgtggcg acaggggaga
     9781 ttattcagtc gattaccacc ggtgcacatc ctgacagcct tatcctctcg cgtgatggca
     9841 caaaagcctg ggtgatgaat cgcaccagta atagtgtgtc agcgattgat ctggtgactt
     9901 atcagcatgt ggcggatatc ccgctggaga aatacgacgg gacggggacg agcggtaaac
     9961 caggcgcctg ggttatggca ctttccccgg atgaaagaac attactggtt ccgggagcag
    10021 gcagaggtaa catcgtgcgg atcaatacca tcacgcatca gaaagaagac tttcccgcag
    10081 gtgatgcgcg tggaacgata tcggcgatgc gttttcgacc tgaaaacggc gaggttattt
    10141 ttgccgatag tcaggggatt tcacgtataa gcgtagggga tcaacaagcc agcattatga
    10201 cgcaatggtg tagcaggagc gtttattccg ttgagggtat tagcccggac ggtcaatatt
    10261 tagcgttggt gtcatatggc ctgcaaggtt atgtcatcct gctcaatatt aatgccgggc
    10321 agattattgg cgtttatcct gccagctacg ttaatcacct tcgtttttca gcggatggta
    10381 gaaaaatatt tgtcatggcg aagaacgggt tgatccaaat ggacaggacg cgctcgcttg
    10441 atccgcaggc aattattcgt catccccaat atggcaatgt ggcttgtatc cctgaaccgt
    10501 aagaaaaagg ggcatgataa ccatgcccca aagctcaatg agaaagtacg ttttgctcat
    10561 gcaggataca tcaggcacat caacgtttac gtgctcgcag actcatcaac acagcggcaa
    10621 aaccaaacag cgccgtcagc acccacagtg gccagttgcc ggtacgtgca tacggtgtga
    10681 gtccggtggt cggtgtcaca ttagtggtca acacctcgcg ggtgaactgc gggatcatcg
    10741 cctgaacctc cccctgcggg ccaatcaccg ccgtaatgcc gttgttggtg ctgcgcaaca
    10801 gtgggcgcgc cagctccagc gcacgcattc gcgccatctg gaagtgctgc catggaccaa
    10861 tagacttacc aaaccacgca tcgttggaga tagtcagcag atagtcggta tccgggcgga
    10921 agttatcgcg cacttgctcg ccgagaatga tctcgtagca aatagccgca gtaagctcaa
    10981 taccatttac cgacagcggc ggctggatat atggcccacg gctgaacgac gacatcggca
    11041 gatcaaagaa cggtgctaac ggacgcagaa tcgactccag cgggacaaac tcgccaaacg
    11101 gcactagatg gtttttgtta tagcgatcgg ctgattcgta gctgtatggt gcacctttac
    11161 ccagtgtgat gatggtgttg taggtatcgt agcggttctg cttattgaga cgcgcgtcga
    11221 caatcccggt taccagcgag ctacctttat cgcgcaactc accgtccagt gctttgagga
    11281 acggttgctg attaatttcc agatcggtta tcgccgactc cggccagata atcaacgatg
    11341 atttgcccat cagcggagcc gttgcgttgt agtaaatctt cagcgtatta agaagctgat
    11401 ctccatccca tttcagtgat tgcggaatat cgccctgaac catcgaaacc tgaatggttt
    11461 tctccggttg cggggtaaac cattggatgt aacgcagcgg gaagggtagg gcaaacagca
    11521 cgacggccac caccagcgga cgccagttgc gtttgaccaa cgccagtgcc agcaggccac
    11581 taaccatcat cagcaggaag ttaatggctt ccacgcccat tatcggtgcc agccctttta
    11641 acgggccatc aatctggcta tagccgaact gtaaccacgg gaagcctgtc agtacccaac
    11701 cgcgcagaaa ctcggtcact tgccagagag caggggcggc aatcgccacg cgcagccagg
    11761 tggttttcgg ccacagacgc gacagcacgc cagcaaacag tccggtatac agcgacaaat
    11821 acgccgccag cagcaccacc aggaagatgt taaccgggcc aggcattccg ccaaaggtcg
    11881 cgatgctgac atagacccag ttaataccgc tgccaaagag gccaaatccc cagcaaaagc
    11941 caatagcggc agactggagt ggacggcggt taaaggtcaa cgcctgaagc cccatcaacg
    12001 aaataatcgc cgcaggccag acgtcgtaag gagagaaggc cagcgttccg caggcaccga
    12061 ataataacgc cagcagcagg cgaatgcgct ggcgttcaat taatgaggca aaagccatgt
    12121 agttatctat ccagtttcgg ttttattcat ccagcttcgg ctggggtgag tcatccggga
    12181 ttttgacatg aacctgaata atacgccgac tgtcggccat cgccactttg aactggtaac
    12241 cgtcgatgtc gatagtttcg ccacgcgccg gaagatgccc aaatgcctgc atcaccagac
    12301 caccgatagt gtcaacctct tcatcgctaa agtgggtgcc gaacgcttcg ttgaagtctt
    12361 caatggaagc cagtgcacgc acggtccagg tatgacgact cagctgacgg aagtcgatat
    12421 catcttcttc gtcatactcg tcttcaatct caccaacaat cagttccagg atgtcttcaa
    12481 tggtcaccag accggaaacc ccaccgaatt cgtcaatgac gatcgccatg tggtaacgct
    12541 gagagcgaaa ctctttcagc atccggtcta cgcgcttact ttcaggaacg acaaccgcct
    12601 gacgtaacac tttgtccatg ctgaaggctt cagcatcgct gcgcataaac ggcagcaagt
    12661 ctttcgccat cagaatccct tcaatgtgat ctttgtcttc gctaatcacc gggaaacgtg
    12721 agtgggcgga ctcgatgatg acatcaaggc attcgtccag cgtctggttg cgtttcaggg
    12781 taatcatctg ggagcggggg atcatgatgt cgcgaacgcg ttggtctgcg atgtccatca
    12841 ccccttcgag catatcgcgc gtatcttcgt cgataaggtc gttctgcccg gaatcacgga
    12901 tcagcgccag cagttcgtca cggtttttcg gttcaccgtg gaaaagttgg ctgagtaaca
    12961 gggagaaaaa tcccttcttg ttgcttatcg tgtcactact gtgtgaattg tcgtcgctca
    13021 tggcgtcgta tgggttctca tgttagttaa tcaaaacgcc gtcgttaatc accaacggcg
    13081 gggacgtctg ccagtcaaat gcctggcaaa ttattctttc tcggcaatgt acggatcctc
    13141 atagcccaga gcaagcataa tctctgtttc gagggcttcc atttcttctg cttcgtcatc
    13201 ttcgatgtga tcgtaaccta acaaatgcag actgccgtgc accaccatat gcgcccagtg
    13261 cgcctccagt ggtttgcctt gctcctgagc ttccttctca accacctgac ggcagataac
    13321 cagatcgccc agtagcgaca tttccatgcc aggcggcact tcaaacggga aggagagcac
    13381 gttggtcggc ttatccttac cgcgataggt cagattcaga ctgtggcttt cggcggtatc
    13441 gaccacgcga atagtcactt ccgattcttc ctgaaactgc gggatcaccg cattcagcca
    13501 tgtttgaaac tggctctctt ccggtaaccc ggaattatct tcacatgcca gttgtaaatc
    13561 gaggatcacc tgactcattt ttgttcctgt tcttcgcgct tgcgttctgc cgccagcgcc
    13621 gcttttcgtt tttgttcggc ttcttcccag gcttcatagg cgttaacgat acgcgccacc
    13681 acagggtgac gaaccacgtc ttcgctgtgg aagaagttaa agctgatctc ttcgacatcg
    13741 gccagcactt cgatggcgtg acgtaagcct gatttagtat tacgcggcag gtcgatctgt
    13801 gtgacgtcgc cggtgataac cgcttttgag ttaaaaccga tacgggtcag gaacatcttc
    13861 atctgttcga tggtggtgtt ctggctctca tcgagaatga taaacgcgtc gttcagcgta
    13921 cgaccacgca tataggccag cggcgcgact tcaataacgt tgcgctcaat cagtttctca
    13981 actttctcaa agcccagcat ttcaaacagc gcgtcgtaca gtgggcgcag atacgggtct
    14041 actttctggc ttaaatcgcc aggcaggaag cccagtttct caccggcttc gaccgccgga
    14101 cgagtcagca gaatacggcg aatctcctga cgctccaggg catcaactgc cgcagccact
    14161 gccaggtagg ttttacccgt acccgccggg ccaacgccga aggtaatgtc atggtcgaga
    14221 atattggcga tgtactgcgc ctggtttggc gtgcgcggct taattacgcc gcgtttggtt
    14281 ttgatattga ccgctttgcc gtactccggc acgctctccg cactttgctc cagtactcgc
    14341 gcttctttaa tcgcaaggtg gatctgttcc ggttcgatat cctgaatctg accgcgcatc
    14401 ggggctgtat cgacatacag gctacgcaga atgtctgccg cagcggtgac gcaaatcgga
    14461 cggccggtca gtttaaagtg gttgtcgcgg cgattgatct cgatgccgag acggcgttcg
    14521 agctgcttga tgttgtcatc aaacgggccg cacaggctca acagacgcgc gttgtctgct
    14581 ggctccaggg tgatttcgcg agtgtctatg ttcaaaccgt tcctcttatc tgtatgccgc
    14641 cggaagctga acattcaccg gcctataagg aaattattca cgccacagga aaaaggcgca
    14701 agcattgcga taaagatggg gataaagaga gaaaaaacaa ggcccaccgg aacggcgggc
    14761 ctgagaatta cggctgataa taacccacgc caaggtcgtt ttctttgcgg gtacgggcaa
    14821 tcaccgattc cggtgtttct gccacgcgca gacccatttc atcttcagta cgcaccactt
    14881 taccgcgcag agagttcggg tagacgtcgg taatttctac atcgacgaat ttaccgatca
    14941 tatccggtgt gccctcgaag ttgaccacgc ggttattttc ggtacgcccg gaaagctcca
    15001 tgatgctctt acgcgaagtc ccttctacca gaatacgctg ggtggtgccg agcatccggc
    15061 ggctccatgc catcgcttgc tgattgatac gttcttgcag aatatacaga cgttgcttct
    15121 tctcttcttc cggaacatca tcaaccatat cggcggctgg tgtacccgga cgtgcagaga
    15181 agataaagct gtagctcatg tcgaaattga cgtcggcaat cagcttcatc gttttctcga
    15241 agtcttcggt ggtttcgcca gggaagccaa caatgaagtc agaactgatc tgaatatccg
    15301 gacgcgccgc acgcagttta cggatgatcg ctttgtactc cagcgccgtg tgagtacggc
    15361 ccatcaggtt cagaatgcga tcggaaccgc tttgtaccgg cagatgcagg aagctcacca
    15421 gctccggcgt gtcgcgatac acttcgatga tatcgtcggt gaattcgatc ggatggctgg
    15481 tggtaaagcg aatacgatcg atcccgtcga tcgcagcaac cagacgcagc agatcggcaa
    15541 acgatccggt ggagccgtcg tagttttcac cacgccaggc gttcacgttc tgaccgagca
    15601 ggttgacttc acgcacgccc tgagccgcaa gctgggcaat ctcaaacaga atatcgtcag
    15661 acggacggct tacctcttca ccacgggtgt aaggcaccac gcagtaggtg caatatttat
    15721 tgcagccttc catgatggag acaaacgcgg tcggcccttc ggcgcgcggt tccggtagac
    15781 ggtcaaactt ctcgatttcc gggaagctga tatctacaac cgggctgcgg tcgccgcgca
    15841 cggagttgat catctccggc agacggtgca gcgtttgcgg cccaaaaata atatcgacat
    15901 agtgggcgcg ctggcgaatg tgctcgcctt cttgcgatgc cacgcagcca ccgacgccga
    15961 taatcaggtc tggattcttc tcttttaaca gtttccagcg acccaactga tggaagactt
    16021 tttcctgagc cttctcgcgg attgagcagg tgttcagcag cagcacatcc gcttcttccg
    16081 ccacgtcggt cagttgatag ccgtgggtgg catccagcag atcggccatc ttcgatgaat
    16141 cgtactcgtt catctgacag ccccaggttt taatatggag ttttttggtc atcgacttgc
    16201 tcttgcgaaa tagtagccag gaatgcaggg cgtatagtgt aatgctttgc tgccgttgtg
    16261 accagtatga gcgttatcag cccttggggg taaaaatcct gtaaacttaa agcatattgc
    16321 taacaggatg attgaccatg acaaatcaac caacggaaat tgccattgtc ggcggaggaa
    16381 tggtcggcgg cgcgctggcg ctggggctgg cacagcacgg attttcggta acggtgatcg
    16441 agcacgcaga acctgcaccg tttgtcgctg atagccaacc ggacgtgcgg atctcggcga
    16501 tcagcgccgc atcggtatca ttgcttaaag ggttaggggt ctgggatgca gtacaggcta
    16561 tgcgttgcca tccttaccgc agactggaaa cgtgggagtg ggaaacggcg catgtggtgt
    16621 ttgacgccgc tgaacttaag ctaccgttgc ttggctatat ggtggaaaac accgtcctgc
    16681 aacaggcgct gtggcaggcg ctggaagcgc atccgaaagt aacgttacgt gtgccaggct
    16741 cgctgattgc attacatcga gataatgatc ttcaggagct ggaactgaag ggcggtgaaa
    16801 cgatccgcgc gaaactggtg attggtgccg acggcgcaaa ttctcaggtg cggcagatgg
    16861 cgggaattgg cgttcatgcc tggcagtatg cgcagtcgtg catgttgatt agcgtacagt
    16921 gtgagaacga tcccggcgac agcacctggc agcaatttac cccggacgga ccgcgtgcgt
    16981 ttctgccgtt gtttgataac tgggcatcgc tggtgtggta tgactctccg gcgcgtattc
    17041 gccagttgca gaatatgaat atggcgcaat tccaggcgga aatcgcgaag catttcccgt
    17101 cgcgtctggg ttacgttaca ccgcttgccg ctggtgcgtt tccgctgacg cgccgacatg
    17161 cgttgcagta tgtgcagccg gggcttgcgc tggtgggcga tgccgcgcat accatccatc
    17221 cgctggcggg gcagggggtg aatcttggtt atcgtgatgt cgatgccctg attgatgttc
    17281 tggtgaatgc ccgcagctac ggtgaagcgt gggccagtta tcctgtcctc aagcgttacc
    17341 agatgcggcg catggcggat aacttcatta tgcaaagtgg tatggatctg ttttatgccg
    17401 gattcagtaa taatctgccg ccgctgcgtt ttgtgcgtaa tctcgggtta atggcggcag
    17461 agcgtgctgg cgtgttgaaa cgtcaggcgc tgaaatatgc gttagggttg tagccttaca
    17521 acattgccgg atgcgtgcca accgtaggtc ggataagacg cggcagcgtc gcatccgaca
    17581 ttgaaggata agacgtgtca acatcgcatt cgacattgaa tgaacgcaga aaagcaaaaa
    17641 actcgccgaa gcgagctttt ttaatgtggc tggggtacga ggattcgaac ctcggaatgc
    17701 cggaatcaga atccggtgcc ttaccgcttg gcgatacccc aacaaattgg tttttgaatt
    17761 tgccgaacgt attcgacaca ttcagaattt ggtggctacg acgggattcg aacctgtgac
    17821 cccatcatta tgagtgatgt gctctaacca actgagctac gtagccagat tgtttcttcg
    17881 atggctgggg tacctggatt cgaaccaggg aatgccggta tcaaaaaccg gtgccttacc
    17941 gcttggcgat accccaataa ccggtcggtg aaccgcttac tcgaagaaga tggctggggt
    18001 acctggattc gaaccaggga atgccggtat caaaaaccgg tgccttaccg cttggcgata
    18061 ccccatccgt acaacgcttt ctggtgaatg gtgcgggagg cgagacttga actcgcacac
    18121 cttgcggcgc cagaacctaa atctggtgcg tctaccaatt tcgccactcc cgcaaaaaag
    18181 atggtggcta cgacgggatt cgaacctgtg accccatcat tatgagtgat gtgctctaac
    18241 caactgagct acgtagccat ctttttttcg cgatacctta tcggcgttgc ggggcgcatt
    18301 atgcgtatag agccttgcag cgtcaacctc tttttcaagg aaaattgctc gaaagtgact
    18361 gtttggttag gttgcgaaca gcgtggcgct atattcgtca attattgttt actttgtgtt
    18421 ttttccccac cctacagcca ttcttttgtc atacaggatg aaattcggaa ttcaacaatg
    18481 gtggtgggaa attaatctat gaaatactgg cctacagtga tgagttgtca aacagtgatg
    18541 tggcaaaccc ggaacatttc cttactgcat atcagaatca acaagcacct caataactga
    18601 aacagccccg gatttcaccg gggctgtttc gcatttctta cttatacgcc gactggtgaa
    18661 cgccaaccgc acgaccagac ggatcgtcca ttttcttgaa cgcctcatcc cactcaatcg
    18721 ctttagcgga agaacacgcg acggacgggc cgcccggcac gcactcagcg gcgctcggaa
    18781 gcgggaacag ttcttcaaag atctcccggt acagatacgc ttcttttgag gttggcgtgt
    18841 tgtacgggaa gcggaagcgg gcagtttcca gttgctgatc agaaacctgc tgcgccgcca
    18901 cttctttcag ggtgtcgatc caactgtaac cgacgccatc ggagaactgc tctttctgcc
    18961 gccaggccac gcttgcgggc aggtatgact caaaacattc acgcaggatg tgtttttcca
    19021 ttttgccgtt accgcacatt ttatcctgcg ggttaatgcg catcgccaca tcaaggaatt
    19081 ttttgtcgag gaacggaacg cgtgcttcca cgccccaggc tgacatcgct ttgttggcgc
    19141 gcgcgcagtc atacatatgc agggccagca gtttacgcac cgtctcttca tgcagttctt
    19201 tagcgttggg cgctttatgg aagtaaagat aaccgccaaa cacttcatca gaaccttcac
    19261 cggacagcac cattttaatg cccatcgcct tgatcttacg cgacattaaa tacatcggtg
    19321 ttgaagcgcg aatagtggtc acatcataag tttcgatgtg gtaaatcacg tcgcggatgg
    19381 catccagacc ttcctgtaca gtgaagtgaa tttcgtgatg caccgtgccc agatggtttg
    19441 ccacttcctg ggctgcttta agatccggtg aacccggcag acctacagca aaggagtgta
    19501 actggggcca ccaggcttca gagcgttcct gatcttccac gcgacgggct gcgtatttct
    19561 tggtgatagc ggaaataatt gaggaatcca gaccaccaga aagcagcaca ccgtaaggca
    19621 catcagacat cagatggctt ttaacggaat cttccagagc ctgacgcagc tcgtttttgt
    19681 cggtcacgtt atctttcact gcatcaaagt cgaaccagtc gcgatgataa taagaacgga
    19741 tttcgccgtc ctggctccac aaatagctcc ccgccgggaa ctctttaatc gtgcggcaaa
    19801 ctggcaccag cgatttcatt tctgaggcca catacagctg accgtgttcg tcatacccca
    19861 tatacagtgg gatgatcccc agatggtcgc gaccaatcag gtaggcatct ttttcgctgt
    19921 cgtacagggc aaaggcaaac atgccctgca agtcgtcgag aaattccggc cctttttcct
    19981 gatacagcgc gaggatcact tcacagtcag accctgtctg gaactgataa cgatcgccat
    20041 attcagcgcg caatgcctgg tggttgtaga tttcaccgtt taccgccagt acgtgggttt
    20101 tttgttggtt gtagagaggt tgcgcccccg cgttaacgtc aacaattgac agacgttcgt
    20161 gagcgagaat ggcgttatcg ctggcataaa taccggacca gtccgggcca cgatgacgca
    20221 tcaggcgtga cagctcgagt gctttcttac gctgctcaac tgcgtctgtt ttgatatcga
    20281 atacgccaaa aattgaacac ataaccttct ccgttaacct ggtatttgtt gcttgttgtg
    20341 tttgcttgtt taaaaaaatg ccgcaaagca gcactgtgcg caagcgattt ggcggtgaaa
    20401 aaataaaaaa cgtaatggtg attgtcgatt ggtgaaaaag gggctggtgt gaggatgtat
    20461 ttattgatga atcgataatt tttagcgggt tttattgaat ggttatattt tacgggg
//
LOCUS       DAZOZY010000058        20423 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZOZY010000058 DAZOZY010000000
VERSION     DAZOZY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20423
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.058"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..368
                     /gene="ydjO"
                     /locus_tag="ACHURQ_001860"
                     /pseudo
     CDS             1..368
                     /gene="ydjO"
                     /locus_tag="ACHURQ_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(372..1763)
                     /gene="tcyP"
                     /locus_tag="ACHURQ_001861"
     CDS             complement(372..1763)
                     /gene="tcyP"
                     /locus_tag="ACHURQ_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR0823647.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1896..2486)
                     /locus_tag="ACHURQ_001862"
     CDS             complement(1896..2486)
                     /locus_tag="ACHURQ_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0823648.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2649..3317)
                     /gene="hxpB"
                     /locus_tag="ACHURQ_001863"
     CDS             complement(2649..3317)
                     /gene="hxpB"
                     /locus_tag="ACHURQ_001863"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR0823649.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3464..4000
                     /locus_tag="ACHURQ_001864"
     CDS             3464..4000
                     /locus_tag="ACHURQ_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR0823650.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4041..4901)
                     /locus_tag="ACHURQ_001865"
     CDS             complement(4041..4901)
                     /locus_tag="ACHURQ_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR0823651.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5007..5297)
                     /gene="ghoS"
                     /locus_tag="ACHURQ_001866"
     CDS             complement(5007..5297)
                     /gene="ghoS"
                     /locus_tag="ACHURQ_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0823652.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5398..6327)
                     /gene="pfkB"
                     /locus_tag="ACHURQ_001867"
     CDS             complement(5398..6327)
                     /gene="pfkB"
                     /locus_tag="ACHURQ_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR0823653.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6614..7372
                     /locus_tag="ACHURQ_001868"
     CDS             6614..7372
                     /locus_tag="ACHURQ_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR0823654.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7425..7532)
                     /gene="yniD"
                     /locus_tag="ACHURQ_001869"
     CDS             complement(7425..7532)
                     /gene="yniD"
                     /locus_tag="ACHURQ_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR0823655.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7704..9602)
                     /gene="espL1"
                     /locus_tag="ACHURQ_001870"
                     /pseudo
     CDS             complement(7704..9602)
                     /gene="espL1"
                     /locus_tag="ACHURQ_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10127..12055
                     /gene="thrS"
                     /locus_tag="ACHURQ_001871"
     CDS             10127..12055
                     /gene="thrS"
                     /locus_tag="ACHURQ_001871"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR0823656.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12059..12601
                     /gene="infC"
                     /locus_tag="ACHURQ_001872"
     CDS             12059..12601
                     /gene="infC"
                     /locus_tag="ACHURQ_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR0823657.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12698..12895
                     /gene="rpmI"
                     /locus_tag="ACHURQ_001873"
     CDS             12698..12895
                     /gene="rpmI"
                     /locus_tag="ACHURQ_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR0823658.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12948..13304
                     /gene="rplT"
                     /locus_tag="ACHURQ_001874"
     CDS             12948..13304
                     /gene="rplT"
                     /locus_tag="ACHURQ_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR0823659.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13421..13545
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13427..13471
                     /gene="pheM"
                     /locus_tag="ACHURQ_001875"
     CDS             13427..13471
                     /gene="pheM"
                     /locus_tag="ACHURQ_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR0823660.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13754..14737
                     /gene="pheS"
                     /locus_tag="ACHURQ_001876"
     CDS             13754..14737
                     /gene="pheS"
                     /locus_tag="ACHURQ_001876"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR0823661.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14752..17139
                     /gene="pheT"
                     /locus_tag="ACHURQ_001877"
     CDS             14752..17139
                     /gene="pheT"
                     /locus_tag="ACHURQ_001877"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR0823662.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17144..17443
                     /gene="ihfA"
                     /locus_tag="ACHURQ_001878"
     CDS             17144..17443
                     /gene="ihfA"
                     /locus_tag="ACHURQ_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR0823663.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17544..18524
                     /gene="btuC"
                     /locus_tag="ACHURQ_001879"
     CDS             17544..18524
                     /gene="btuC"
                     /locus_tag="ACHURQ_001879"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR0823664.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            18587..19138
                     /gene="btuE"
                     /locus_tag="ACHURQ_001880"
     CDS             18587..19138
                     /gene="btuE"
                     /locus_tag="ACHURQ_001880"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR0823665.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19138..19887
                     /gene="btuD"
                     /locus_tag="ACHURQ_001881"
     CDS             19138..19887
                     /gene="btuD"
                     /locus_tag="ACHURQ_001881"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR0823666.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19964..>20423
                     /locus_tag="ACHURQ_001882"
     CDS             19964..>20423
                     /locus_tag="ACHURQ_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR0823667.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         5262 a         4776 c         5210 g         5175 t
ORIGIN      
        1 ttatatacat cctgccggga gcatttttgc tgcgattcaa aaaaaatgag agtctcctaa
       61 agtttccttc gataatatca atgcttaata tcaatcttct ttttaatcca ctaaatcttc
      121 cgggaatggg aagtggtgtg cttgaagata ttatgagtat tccagatagt tcacttagaa
      181 aacgtctcgg gtatgaggtt ttaagcttct cactacaggc acattcattg agccaggaat
      241 gtattgataa gttggacatt tttttcgctg acgatctgtt taaatatgag tcagtatgca
      301 tcgccgcgat ggaacattta aaaagtaaag caacggcccc tatacaaaac ggaccgttgc
      361 cagcataaga attaacggtg tgccagttcg gcgtcgtctt cgctatccag aatggcttta
      421 tcggtttgct tcagccactg gctggtcagc gtgccagctg tcatcgagcc actaacgttt
      481 agcgccgtac ggcccatgtc gataagcggt tcaacggaga ttaacagcgc caccagggtt
      541 actggcaggc ccatcgcagg cagcacaatc agtgcggcga aagttgcacc accaccgaca
      601 ccggcaacgc ctgcggaact aacggtaaca ataccgacca gcgtcgcaat ccacatcggg
      661 tccagcgggt taatgccaac cgtaggcgca accatcaccg ccagcattgc cggatacaaa
      721 ccggcgcagc cgttctgacc aatggttgca ccgaaagagg cggcgaaact ggcgatggat
      781 tcaggaacgc ccagacgacg cgtttgtgct tccacattca gtgggataga cgcagcactg
      841 gaacggctgg taaaggcaaa cgtcagcaca ggccatacct tacggaagta cttcagcgga
      901 ctcacgccat taatgcccag cagaatgcca tgcactgcaa acataatcag cagaccgagg
      961 taggacgcga caacgaaact tcccagtttg atgatgtctt gcaagttaga acctgcaacc
     1021 actttggtca ttagagccag aacgccgtaa ggggtcaact gcatgacaag gcgaaccagt
     1081 ttcatcaccc agctttgtag ggtatcgata gcggctaaga cgcgttcacc tttcggcgca
     1141 tcatccttca gcagtttcaa tgcagctacg ccgaggaatg cggcaaaaat taccacgctg
     1201 ataattgacg tcggattggc tccggtaaga tcggcaaacg ggtttttcgg gataaaggac
     1261 aagaccagct gcggaacgct cagatcagag actttaccaa catagttact ttcaatggcg
     1321 ttcagacgtg cagtttctgc accaccctga accagacctt cagccgtcaa accaaacagg
     1381 ttggtgacca gcacaccgac cagcgccgca atcagcgtgg taaacaaaag cgtaccgatg
     1441 gtcagaaaac tgattttgcc taactgagat gcgttatgca gacgggcaac cgcgctcaga
     1501 atagaggcga agactaacgg cataacgatc atttgcagca gttgaacata gccgttacca
     1561 acgatgttaa accactgtac agaatcttta agtacctggc tgtcagaacc ataaatggta
     1621 tgcagggcaa ggccaaaaac cacacccatc accagaccca ccagcacttt tttcgccaga
     1681 ctccactgtt tatggcgggt ctgagccagc gcaaacagca gtacaacgaa caccacgatg
     1741 ttcgcaatta atggaaagtt catccccgtt ctcctgatct tattatttct atcactgcgt
     1801 agcagtcata gtgttggcgc aagattaaca gaactgtgag ctggctctta tattcaaatg
     1861 gaattgctta ttccagaaag gtgatttttg cctttttagt attcaacctg atgcttaata
     1921 agccgatgaa actggatctg caaggtattg atcatttgcg atgaccaacg aacagcgctg
     1981 ttctcgggta atgaatgggg catccatacc gaccagacaa ataatgccat gcagataaaa
     2041 cgttccagtt ggttgccctt ttggggaacc aggataggca agcggaaacg ccagcggcat
     2101 ggccagagca ggggaacacc ggcgggtgtc agcatatcgg caagtatgtg gctcaaataa
     2161 cccagcacca ttccttgtag cgcatcagcc ggaatgaacc agccttccgg aaccttaagg
     2221 taaaaggttg ccagcagcgc aaataccgcc agcagactgt gggtaaaacc acgatgccca
     2281 aaagcgcggg cgatcggttt tgatatccat tttaatcgct gcccaagaaa cgactttggg
     2341 tgatcgatgt ccggtaacaa acacgtcagg attgcggaag ggacaatatg ccaccagtca
     2401 ccctgtgcca gcacgggcgt cagctcggca tttttggcaa ataccgcaca agcaatagaa
     2461 aagagaaggt gaccttccgc cgtcatgata aaactcacta aactgtcgat acgtacagta
     2521 taggattttt atacagtgca cggaagtggt gacggtgtaa ctctttctct taaattgacg
     2581 attaatccgt cagttgaatg caaattcaga taaagaacca ggggcagcgt gatcactgcc
     2641 cctggtcatt aaccgagaag gtcttttgcg gtgagttctg tcagcgatga gagtttgacg
     2701 tttgctaata caaaacgtgg atcattttgc gcttctggcg caggaacgac gatggaacgc
     2761 atgcgggctg ctttagaagc gatcatgcca tttaccgagt cttccagcgc cacgcaggtc
     2821 agtgggtcaa cgcccagttt tgctgcgcag tcgagatata cttgcggatg cggcttgctg
     2881 taaggcagtt tttcagccga ggcgagggca tcgaaactgt cgcgtaagtc aaacatggtc
     2941 aacacttttt ccagcatatg tagtggtgac gcggaggcca gtcccaccaa taaaccttgt
     3001 tctttgcata acgcaacagc ttcgcgcacg cctggtaata atggacgtgt ctcttcaacc
     3061 agtgaaatgg cacgggcaat aacccgttct actacttcct gacggcttgg cccattccac
     3121 ggttgccggg cgtaccaaag atcgaccacc atatcgatgc gtaaacctaa ggtgtccggc
     3181 agctcgttac gacgggagat atccaccccc aggcttgcca tcacatccag ttcggctcga
     3241 tcccataaag gttctgagtc gataagtaat ccatccatat caaaaattgc agcaagaatc
     3301 tgacgcgggg ttgacattac aacctctcct ttagagcatt atgtgtaagg tattaagccg
     3361 tgcttatgat taagcgccta tcatatcgcc tttaaagaat gggcgaaaac agatatctac
     3421 aggtaaacta agtgaaacga cgcgtcttaa tcaaggagaa ctcatgacgt atcaacaagc
     3481 tggacgcatt gctgttttga aaaggatttt gggttgggtg atttttatac ctgcattgat
     3541 ctccacattg atttctttgc tgaaatttat gaatacccgg caggaaaacc aggaaggcat
     3601 taatgcggtc atgctcgact ttactcatgt catgatcgat atgatgcagg cgaatacgcc
     3661 ttttttgaat ctgttctggt ataactcccc gacccctaat tttaatggtg gcgtgaacgt
     3721 tatgttctgg gtgattttca tcctgatttt tgtcggactg gcgttgcagg attccggtgc
     3781 ccggatgagt cgccaggcgc gcttcttgcg ggaaggtgtt gaagatcaac tcattctgga
     3841 aaaagccaaa ggggaagagg ggctaacgcg tgaacaaatt gagtcccgta ttgttgttcc
     3901 gcaccatact attttcctgc agtttttctc actatatatc ctgccggtta tttgtattgc
     3961 tgcaggttat gtgttctttt ctctgcttgg gtttatttaa tcaaaaggcc gcacggagcg
     4021 gccattatcc tcaacccata tcatgctgct aataatctat ccaatgactg ctgagcaata
     4081 accaaatgct gaccgccaaa taatcttgca cgatttaaca gcgtgtagag ttggtaaacc
     4141 ggttgacgtt caaggaaatc ggcaggtagc ggggatactg actgatagcc gtcatagatt
     4201 tgtggcggct gttcagtatg cagcggtaac atcgccaggt cgcactctcg gtcaccccag
     4261 tagcaggcag ggtcgaaaat gtacgggcca tccggaccca gcgcacagtt gccggaccat
     4321 aaatcgccgt gcaacagaga aggctgcagt tgatgcgagg ccagacgctg ctgaatatgc
     4381 tcgacgatgg cgtcgatatt gccgaaagcg atccctttct ctgctgccag ttccaactgc
     4441 cagccaatcc gttgttcagc aaaaaacgtt gaccagcgac gttgccaggt gttgggctgt
     4501 ggagttgtgg agagcgagtt atcaaaatcg aggccaaatt gtggttggtc actccattga
     4561 tgtaaacgcg caatttgctg accaagaata aatgcgctat gcgcatccag tggacgaggt
     4621 gggagataat ccatcaccag aaaactgtag tcacggtcag cgcctactgc ccaaacctta
     4681 ggcacggtga cggttttact acgcgacagt aactccagtt ggtcggcttc ggcggtaaaa
     4741 ccgggaagca gttccctttc atcacatttg acgaaaaagt catgtcctgc atagcgcaaa
     4801 tgccatgcgg catggacttc tccgccaggc agttcattac gcagttcgat ttcgccttca
     4861 cctaactgct cgctcaaaag acgactgatt gcctgccaca tgatgtctct cccatgttgt
     4921 ctgccagatc ataaggttag cgcaagaatg cggtgtaaaa acacgagcta acgcacacca
     4981 cagactgatt attgcccaaa aaccatttat tttctgttgc tattccattc ctcccaagtc
     5041 gtgatggtga tttcaggatc tttgccggtt gcactttggg taagccccga aaccagttct
     5101 ctaatttctt cttcactttg ggtactgata agtccaaaag tgttagtccc cagctcatgg
     5161 atatttccct catcatccgt catggtgagc aaaaaaccgt cgcgagtcag gtaattattc
     5221 aactcgttaa tttcagtcaa tgtatcctca tgcaacatga cggttatgac ataacggaca
     5281 agatcgccac tggccatagt tcaccccttt gttatcacat gacttttgag caaagtcgga
     5341 gaaacgcgtt gccgacaggt tggtggtgat tcccccaatg ctgggggaat gtttttgtta
     5401 gcgggaaagg taagcgtaaa ttttttgcgt atcgtcatgg gagcacagac gtgttccctg
     5461 attgagtgtg gctgcactcc ccgcagctac gccaaaacgg accatctctt caagagaggc
     5521 attttctgcc agtttcagtg tcatcgcgcc gaccatgctg tcaccagcgc caacggtact
     5581 ctggcttttc accggtggtg gcaccacctg aatgcagttt tcactatcaa cacccagcgc
     5641 tccttgtgga ccaagggaaa cgacaacccg tttggccttg ccgctattaa cgatttcctg
     5701 cgcggctttg cggacatcgt ccggctgggt gagttcgcga ttcaccagcg cactgagttc
     5761 tttttggtta ggcttaacca actcgatgtt accaattgcc agtgctgcac ttaacgcttc
     5821 gccagaactg tcgacgatgc agcggatccc ttgtttttgc gaagcggaaa tcagttgggt
     5881 taatttttcc agcttcacac ctggcggcag gcttccgctt atgaccagga tggccccgga
     5941 ttcaatttcc agaacttgct cttcaagctg gcgaaactca tcttcattta atgccgcgcc
     6001 tggcataaca aaacgatact gctcaccgct tgcttccaca tgtacatgta aattctgccg
     6061 ggtccagtct ttggcttcta cagtagcgac ggggacattt tcatccgcca acagtgaaac
     6121 caggtgttcg ccggtcgcgc cacccgccgg gaagatcgct gtggcactgc ctccaagatg
     6181 ggcaatggcg cgggcgacgt tgatgccgcc gcccccgggt tcgaacaccg gtgcggtaca
     6241 gcgcagtttt ccttcgggat aaatttgcgg ggtaattgtt gcgctatcga gagagggcgc
     6301 aagtgtcaac gtatagatac gtaccatcat ttcctcctat aggctgattt cagtctggca
     6361 ccgaatcagc ggaaagtgaa gaaattaaca atatgattta ttggagcttt atttaaaatt
     6421 ttgcagataa atatatataa ataaaaatct gttttcgcca catgcttatt caaaaaatga
     6481 aataagaata cattgctaag ttattcgcgc ctttttataa ttcgttacct ttccagggac
     6541 gctaatcttt gatccttaag aaagtttccg ggaccgcgat ggtctctttt tttgttgtac
     6601 ggactcttga taaatgaagc ttttgaagac agttcctgcc atcgttatgt tggcgggggg
     6661 catgtttgcg tcactgaatg cagctgccga tgattccgtt tttactgtca tggacgatcc
     6721 tgccagcgct aaaaaacctt tcgaaggtaa tctgaacgca ggatatcttg cacaatcagg
     6781 caatacgaaa agctcctcac ttacggctga caccaccatg acctggtatg gacaaaccac
     6841 tgcctggtcg ctgtggggga acgccagtaa tacttcttct aatgatgagc gttcttcaga
     6901 aaaatatgcg gcgggcgggc gtagccgttt taatttaact gattatgact atttatttgg
     6961 tcaggcaagc tggctgacag accgttataa cggctatcga gagcgcgatg tgttgaccgc
     7021 gggttatggt cgccagtttt tgaatggccc ggtacacagt ttccgctttg aattcggccc
     7081 aggtgtgcgt tacgacaaat ataccgacaa tgccagtgaa acccagccgc tgggctatgc
     7141 ctccggggct tatgcatggc agttgactga caacgctaaa tttacgcagg gtgtttcggt
     7201 ctttggcgcg gaagatacaa cgctgaactc cgaaagcgca ttaaatgttg ctattaatga
     7261 acattttgga ctgaaggtgg cttacaacgt cacatggaac tctgaacctc cagagtctgc
     7321 accggaacat accgatcgtc ggacaacctt atcgctgggc tacagcatgt aaaattatct
     7381 gccggaccgt gttatgcggt ctggctgtct tcgtttgtca ctgctcagcc ccaaatcatt
     7441 gctgcccaac gtagcaacaa catagcgcca cagattgcca gtagcaccac caccatcttc
     7501 cagtgttttt tatcaaagcg tttagtcggc ataccagtgt cctgcatatc tcttgtgcgt
     7561 gataacggag tgtaacaaaa gggataggca tcaagggatt ttttgttatt acagccgtcg
     7621 ggtggcgttg tgcacatggt cactgtttct gcgttttatg aaaaaaataa aattatgtat
     7681 cgatatatat cgtaaatagc tggttataaa tacgtcttta tttcatcagc actaaaactc
     7741 aggctttggc tgatgacatt tatagcagca taatatgttt ctacagtttt ataatgctta
     7801 tgatgaatgg ctatatgaac agctgacatg ttgtcatgat ttttagcggc caatagtgta
     7861 aataactgat gttgactaaa agaatgtttt tttgcaaaag cacccagcgt cgatatataa
     7921 gataacacga cgtcttcatt acccttgctc atggcgatgt ataatgcagg ggttccctgt
     7981 gctgtagctc cttttaataa atccatgatg ttagctttgc tcaacttgta tttaaaggcg
     8041 atgccattaa ttttagagag gaaccgtgtg acacacaaag ggtgattatt ttccattgcg
     8101 gcgtataatc ctggctcacc atcatgtatt ttcatggcta aaatactcat caccattttt
     8161 ttgtttaaaa gttcttgtgc tattggagtg gttaatgcat tcagaaagat atcgattatt
     8221 tcatcgaatc catacaacat ggagatatag agtccaggtg tttcgttagc acttttagtt
     8281 tgcagtaatt taaccagatt atcttcatga atgatatgat tgtataccag tgactgaatt
     8341 tcttgcataa atatttttac gttgtcagtt tgtccgttca tcatagccag aaataaagca
     8401 ggtgtaccaa cgctattctg cgcagttatt atttcaatga tttctttttc agggagtatg
     8461 ggcaactgtt tgaatagtgt aataagctat tgagtctggc ctgtggccat tattaacaac
     8521 attgccgatg gatgtatagg ggcaaatggc attcgtgtcg tgatacccgc taaaatagca
     8581 actggaactc cctcaggtaa tagaggaaga atagcaattg cacagttgcg aacataccta
     8641 tcagcccagg tctgagcatc gggagctgaa ctgatgaact tatcaagcga gagaaagccc
     8701 cgtgctgccc tgtgggtgcc tctgacggca acgttggtat cgttcgggtc gtaaacagac
     8761 acaacataat ttaattgtcc atacactgac tgctttattc gtagccttac tgacaaagta
     8821 tgattgtcta tatataaaat cgcggacata tgactgacat tgtttgccat cattcgacaa
     8881 aagaaaatat ctaaagcgaa gccaaaatcc ttactcgcga taacgcgact ccctgcagga
     8941 ggatggcgaa gtaatgctct gaactccgcg tcccggacta aggggacatg ctgctgtatg
     9001 gattcaacgg aggagtagtg tttaatcagg ttgactttgc cttttccatt tctcaatgaa
     9061 tcaagggcgt attgcaatga cagatggcga caaaaaatag cgtcagaagg agattgcgaa
     9121 aaaaatgcac taccattgag atttactgtt ggttgtatag ttttttctct atggattttt
     9181 ttagacagtt tcatacaatg gttacgatct tcggcagtta actgttcctg ttcaatataa
     9241 tccagtatgc ttcgcaaatg gttagcctta ataggcgtgg tttccagaaa taagggaatg
     9301 atttcttttt tatagtgtaa tgtaatgagt atattttctg catggatggg tgaagtggca
     9361 aagttaatga taaaaagggc agtggaataa caattttgtg aaatttttcg acagtttgcg
     9421 gtaaattcct gagtaacaca gtatgacagc atcgaactta agtcagaggt atcaagtagg
     9481 gtgatgagtt ggcgcgcaaa ttgattaata ggctctgatg aaggcgttgg tatgtcgcta
     9541 tcgatgtgca atctatgagg agacttataa tgggttctga taatgatatc gttttgactc
     9601 ataatctatt cgccttatat atgtctggag gttttgagga tgatgattaa ttattatcgc
     9661 cagaacgaaa tgtgcacgaa aaattgctat aagaattgtg tcacttttat cattgtgata
     9721 tgaccacttc gtcattaatc tcatgagcgt ttaaaatact accgccaaat cacgcaacga
     9781 actttgctgg attcagttta tgctgctgta aatccgctcg cagtaaacct ttcagacgca
     9841 ccgtgatgtt atcagtggtt ctttaagcgt tttgctggtg tactcactac aaacgaattg
     9901 cgaatcgaat caatgtgaaa cggaaaggta caatctccct ttttttagtg ttaacatcgt
     9961 tcaaccgggt ggaaggtgag aggttaatgt ctttcatttg tgtacctgat tgcgaaccaa
    10021 tttagcattt gttggctaga tggtttcgca atgaactgtt aataaacaaa tttttctttg
    10081 tatgtgatct ttcgtgtggg tcaccactgc aaattaagga tataaaatgc ctgttataac
    10141 tcttcctgat ggcagccaac gccattacga ccacgctgta agccccatgg atgttgcgct
    10201 ggacattggt ccaggtctgg cgaaagcctg tatcgcaggg cgcgttaatg gcgaactggt
    10261 tgatgcttgc gatctgattg aaaacgacgc acaactgtcg atcattaccg ccaaagacga
    10321 agaaggtctg gagatcattc gtcactcctg tgcgcacctg ttagggcatg cgattaaaca
    10381 actttggccg cataccaaaa tggcaatcgg cccggttatt gacaacggtt tttattacga
    10441 cgttgatctt gaccgcacgt taacccagga agatgtcgaa gcactcgaga agcggatgca
    10501 tgagcttgct gagaaaaact acgacgtcat taagaagaaa gtcagctggc acgaagcgcg
    10561 tgaaactttc gccaaccgtg gggagagcta caaagtctcc attcttgacg aaaacatcgc
    10621 ccatgatgac aagccaggtc tgtacttcca tgaagaatat gtcgatatgt gccgcggtcc
    10681 gcacgtaccg aacatgcgtt tctgccatca tttcaaacta atgaaaacgg caggggctta
    10741 ctggcgtggc gacagcaaca acaaaatgtt gcaacgtatt tacggtacgg cgtgggcaga
    10801 caaaaaagca cttaacgctt acctgcagcg cctggaagaa gccgcgaaac gcgaccaccg
    10861 taaaatcggt aaacagctcg acctgtacca tatgcaggaa gaagcgccgg gtatggtatt
    10921 ctggcacaac gacggctgga ccatcttccg tgaactggaa gtgtttgttc gttctaaact
    10981 gaaagagtac cagtatcagg aagttaaagg tccgttcatg atggaccgtg tcctgtggga
    11041 aaaaaccggt cactgggaca actacaaaga tgcaatgttc accacatctt ctgagaaccg
    11101 tgaatactgc attaagccga tgaactgccc gggtcacgta caaattttca accaggggct
    11161 gaagtcttat cgcgatctgc cgctgcgtat ggccgagttt ggtagctgcc accgtaacga
    11221 gccgtcaggt tcgctgcatg gcctgatgcg cgtgcgtgga tttacccagg atgacgcgca
    11281 tatcttctgt actgaagaac aaattcgcga tgaagttaac ggatgtatcc gtttagtcta
    11341 tgatatgtac agcacttttg gcttcgagaa gatcgtcgtc aaactctcca ctcgtcctga
    11401 aaaacgtatt ggcagcgacg aaatgtggga tcgtgctgag gcggacctgg cggttgcgct
    11461 ggaagaaaac aacatcccgt ttgaatatca actgggtgaa ggcgctttct acggtccgaa
    11521 aattgaattt accctgtatg actgcctcga tcgtgcatgg cagtgcggta cagtacagct
    11581 ggacttctcc ttgccgtctc gtctgagcgc ctcctatgtg ggcgaagaca acgagcgtaa
    11641 ggtaccggta atgattcacc gcgcaattct ggggtcgatg gaacgtttca tcggtatcct
    11701 gaccgaagaa ttcgctggtt tcttcccgac ctggcttgcg ccagttcagg ttgttatcat
    11761 gaatattacc gattcacagt ctgaatacgt taacgaattg acgcaaaaac tatcaaatgc
    11821 gggcattcgt gttaaagcag acttgagaaa tgagaagatt ggctttaaaa tccgcgagca
    11881 cactttgcgt cgcgtcccat atatgctggt ctgtggtgat aaagaggtgg aatcaggcaa
    11941 agttgccgtt cgcacccgcc gtggtaaaga cctgggaagc atggacgtaa atgaagtgat
    12001 cgagaagctg caacaagaga ttcgcagccg cagtcttaaa caattggagg aataaggtat
    12061 taaaggcgga aaacgagttc aaacggcgcg ccctaaccgt atcaatggcg aaattcgcgc
    12121 ccaggaagtt cgcttaacag gtctggaagg cgagcagctt ggtattgtga gtctgagaga
    12181 agctctggag aaagcagaag aagccggagt agacttagtc gagatcagcc ctaacgccga
    12241 gccgccggtt tgtcgtataa tggattacgg caaattcctc tatgaaaaga gcaagtcttc
    12301 taaggaacag aagaaaaagc aaaaagttat ccaggttaag gaaattaaat tccgtcctgg
    12361 tacagatgaa ggcgactatc aggtaaaact ccgcagcctg attcgctttc tcgaagaggg
    12421 tgataaagcc aaaatcacgc tgcgtttccg cggtcgtgag atggcgcacc agcaaatcgg
    12481 tatggaagtg cttaatcgcg tgaaagacga tttgcaagaa ctggcagtgg tcgaatcctt
    12541 cccaacgaag atcgaaggcc gccagatgat catggtgctc gctcctaaga agaaacagta
    12601 aggccttcaa gtagcactgt ctgtggagcc ttcgggcttc acaggctttg ttcgcctttg
    12661 tttcgtttat taacaatgcg aagtggaagt tattaaaatg ccaaaaatta agaccgtacg
    12721 cggtgctgct aagcgcttca aaaaaaccgg taaaggtggt tttaagcaca agcacgctaa
    12781 cctgcgtcac attctgacca aaaaagcgac caaacgtaaa cgtcacctgc gtccgaaagc
    12841 catggtttcc aaaggcgatc tgggcctggt aatcgcgtgc ctgccgtacg cataagccgt
    12901 taacgttttt aactttttaa ttagaatata gatacaggag agcacatatg gctcgcgtaa
    12961 aacgtggtgt tattgcacgc gcacgtcaca agaaaatttt gaaacaagct aaaggctact
    13021 acggtgcgcg ttctcgcgta taccgcgttg ccttccaggc tgttatcaaa gctggtcagt
    13081 atgcttaccg tgaccgtcgt caacgtaagc gtcagttccg tcaactgtgg attgcgcgta
    13141 tcaacgcagc agcacgtcag aacggtattt cttacagcaa attcatcaat ggcctgaaaa
    13201 aagcctctgt tgaaatcgac cgtaagatcc tggctgatat cgcagtattc gacaaagtag
    13261 cgttcaccgc tctggttgaa aaagcgaaag cagctctggc ataagccagt tgaaagaggg
    13321 agctagtctc cctcttttcg tttcaacgcc atcaaaacat tgacttttat cgccgtagcc
    13381 ttttcaataa aggtcttttg aagagtacca aaaggtaacg caagcaatga atgctgctat
    13441 tttccgcttc tttttttact ttagcacctg aatccaggag gctagcgcgt gagaagagaa
    13501 acggaaaaca gcgcctgaaa gcctcccagt ggaggctttt tttgtatgcg cgttttgaaa
    13561 aataaaaagc cagtctatca ggcgtaattg tcgcagccag tctggacacg gccagcgcgg
    13621 agaaaccgga gcgtatttaa gtacgtgaga atttcgagca ctgcccggga ccaaaatggc
    13681 aagtaaaata gcctgatggg ataggctcta agtccaacga accagtgtca ccactgacac
    13741 aatgaggaaa accatgtcac atctcgcaga actggttgcc agtgcgaagg cggccattag
    13801 ccaggcgtca gatgttgccg cgttagataa tgtgcgcgtc gaatatttgg gtaaaaaagg
    13861 gcacttaacc cttcagatga cgaccctgcg tgagctgccg ccagaagagc gtccggcagc
    13921 tggtgcggtt atcaacgaag cgaaagagca ggttcagcag gcgctgaatg cgcgtaaagc
    13981 ggaactggaa agcgcagcgc tgaatgcgcg tctggcggcg gaaacgattg atgtctccct
    14041 gctaggtcgt cgcattgaaa acggcggtct gcatccggtt acccgtacca tcgaccgtat
    14101 cgaaagtttc ttcggtgagc ttggctttac cgtggcaacc gggccggaaa tcgaagacga
    14161 ttatcataac ttcgatgctc tgaacattcc tggtcaccac ccggcgcgcg ctgaccacga
    14221 cactttctgg tttgacacta cccgcctgct gcgtacccag acctctggcg tacagatccg
    14281 caccatgaaa gcccagcagc caccgattcg tatcatcgcg cctggccgcg tttatcgtaa
    14341 cgactacgac cagactcaca cgccgatgtt ccatcagatg gaaggtctga ttgttgatac
    14401 caacatcagc tttaccaacc tgaaaggcac tctgcacgat ttcctgcgta acttctttga
    14461 ggaagatttg cagattcgct tccgtccttc ctacttcccg ttcaccgagc cttctgcaga
    14521 agttgacgtc atgggtaaaa acggtaaatg gctggaagtg ctgggttgcg ggatggtgca
    14581 tccgaacgtg ctgcgtaacg ttggcatcga cccggaagtt tactctggtt tcgccttcgg
    14641 gatggggatg gagcgtctga ctatgttgcg ttacggcgtc accgacctgc gttcattctt
    14701 cgaaaacgat ctgcgtttcc tcaaacagtt taaataaggc aggaatagat tatgaaattc
    14761 agtgaactgt ggttacgcga atgggtgaac ccggcgattg atagcgatgc gctggcgaat
    14821 caaatcacta tggcgggcct ggaagttgac ggtgtagaac cggttgccgg tagcttccac
    14881 ggcgtggtcg ttggtgaagt ggttgagtgt gcgcagcatc cgaacgctga caaactgcgt
    14941 gtgacaaaag tgaatgtcgg cggcgatcgc ctgctggaca tcgtctgtgg tgcgccaaac
    15001 tgccgtcagg gcctgcgtgt ggcggtagct accattggtg ctgttctgcc gggtgatttc
    15061 aaaattaaag cggcgaaact gcgtggcgaa ccgtctgaag ggatgctgtg ctccttctct
    15121 gaactgggaa tttctgacga tcacagcggc attatcgaac tgcctgcaga tgcgccgatt
    15181 ggcactgaca tccgcgaata cctgaaactc gatgacaaca ccatcgaaat cagcgtaacg
    15241 ccaaaccgtg ccgactgctt aggtatcatt ggtgttgcgc gtgacgttgc cgtgctgaat
    15301 cagctgccgc tggttgaacc ggaaatcgtt ccggttggtg cgaccatcga cgacacgttg
    15361 ccgattacag tcgaagcgcc tgaagcctgc ccgcgctatc ttggccgtgt ggtaaaaggc
    15421 attaacgtta aagcgccaac tccgctgtgg atgaaagaaa aactgcgtcg ttgcgggatc
    15481 cgttctatcg atgcagttgt tgacgtcacc aactatgtac tgctcgaact gggccagccg
    15541 atgcacgctt tcgataaaga tcgcattgaa ggcggcattg tggtgcggat ggcaaaagag
    15601 ggcgaaacgc tggtgctgct tgatggcact gaagcgaagc tgaatgctga cactctggtc
    15661 atcgccgacc acaacaaggc gctggcgatg ggcggcatct ttggtggcga acactctggc
    15721 gtgaatgacg aaacgcaaaa cgtcttgctg gaatgcgctt tcttcagccc gctgtctatc
    15781 accggtcgtg ctcgtcgtca tggcctgcat actgatgcgt ctcaccgtta tgagcgtggc
    15841 gttgatccgg cactgcagca caaagcgatg gaacgtgcga cccgtctgct gatcgacatc
    15901 tgcggtggtg aggctggtcc ggtaattgat atcaccaacg aagcaacgct gccgaagcgt
    15961 gcaaccatca ctttacgtcg tagcaaactg gatcgcctga tcggccatca tattgctgat
    16021 gaacaggtaa ctgacattct gcgtcgtctc ggctgcgaag tgaccgaagg caaagacgag
    16081 tggcaggcag ttgcgccgag ctggcgtttc gatatggaga ttgaagaaga tctggttgaa
    16141 gaagtcgcgc gtgtttacgg ctacaacaac atcccggatg agccggtaca ggcaagcctg
    16201 attatgggta ctcaccgtga agctgacctg tcgctcaagc gcgtgaaaac gctgctcaac
    16261 gacaaaggct atcaggaagt gatcacctac agcttcgttg atccgaaagt gcagcagatg
    16321 atccatccag gcgttgaagc cttactgctg ccaagcccga tctctgttga aatgtcagca
    16381 atgcgtcttt ctctgtggac cggcctgctg gcaaccgtgg tgtacaacca gaaccgtcag
    16441 cagaaccgtg tgcgcatttt cgaaagcggt ctgcgtttcg taccagatac tcaggcaccg
    16501 ttgggcattc gtcaggatct gatgttagcc ggtgtgattt gcggtaaccg ttacgaagag
    16561 cactggaacc tggcaaaaga gaccgttgat ttctatgatt tgaaaggcga tcttgaatcc
    16621 gttctcgacc tgaccggtaa actgaatgag gttgagttcc gtgcagaagc gaatccggca
    16681 ctgcatccgg ggcaatccgc agcgatttat ctgaaagggg aacgtattgg ttttgttgga
    16741 gttgttcatc ctgaactgga acgtaaactg gatcttaacg gtcgcactct ggtgttcgaa
    16801 ctggagtgga acaagctcgc agaccgcgtg gtgcctcagg cgcgcgagat ttctcgcttc
    16861 ccggcgaacc gtcgtgacat cgcggtggtg gtcgcagaaa acgttcccgc agcggatatt
    16921 ttatccgaat gtaagaaagt tggcgtaaat caggtagttg gcgtaaactt atttgacgtg
    16981 taccgcggta agggtgttgc ggaggggtat aagagcctcg ccataagcct gatcctgcaa
    17041 gataccagcc gtacactcga agaagaggag attgccgcta ccgtcgccaa atgtgtagag
    17101 gcattaaaag agcgattcca ggcatcattg agggattgaa cctatggcgc ttacaaaagc
    17161 tgaaatgtca gaatatctgt ttgataagct tgggcttagc aagcgggatg ccaaagaact
    17221 ggttgaactg tttttcgaag agatccgtcg cgctctggaa aacggcgaac aggtgaaact
    17281 ctctggtttt ggtaacttcg atctgcgtga taagaatcaa cgcccgggac gtaacccgaa
    17341 aacgggcgag gatattccca ttacagcacg gcgcgtggtg accttcagac ccgggcagaa
    17401 gttaaaaagc cgggtcgaaa acgcttcgcc caaagacgag taatctgatc taactaaaaa
    17461 ggccgctctg cggccttttt tcttttcact gtcgaagagt caccgtaaaa tcaacgccat
    17521 gacacttcag cagaacggat accatgctga cacttgcccg ccaacaacag cgacaaaata
    17581 ttcgctggtt attatgcctg tcagttttga tgctgctggc gcttctctta agcctttgcg
    17641 ccggtgaaca atggatttcg ccaggtgact ggtttactcc tcgtggcgaa ctgttcgtct
    17701 ggcaaattcg cctgccacgt acgctggctg tattgctggt tggtgcggcg ctggctatat
    17761 ccggcgctgt aatgcaggcg ttgtttgaaa atcctctggc agaacctgga ctacttggcg
    17821 tctctaacgg cgcaggcgtg gggcttatcg ccgcggtatt gcttgggcaa gggcaactcc
    17881 ccaactgggc gctagggctg tgtgcgattg ctggcgcgct tatcatcact ttaatactct
    17941 tacgtttcgc ccgtcgtcat ctttcgacca gtcggttatt gctggctggc gttgcattag
    18001 ggattatctg tagcgcacta atgacgtggg ctatctactt ttccacctct gttgatttac
    18061 gtcagctgat gtactggatg atgggcggtt ttggcggcgt agactggcgg caaagctggc
    18121 tgatgctggc attgatcccc gtgttgttgt ggatctgttg tcagtccagg ccgatgaata
    18181 tgttagcact tggcgagatc tcggcgcggc aactgggttt acccctgtgg ttctggcgca
    18241 atgtgctggt ggcagcgacc ggctggatgg ttggcgtcag tgtggcgctg gcgggtgcta
    18301 tcggctttat tggtctggtg atcccacata ttcttcggtt gtgtggttta accgatcatc
    18361 gcgtattact tcccggctgc gcgctggcag gggcgagcgc agtgctgctg gccgatattg
    18421 tagcgcgcct ggcattagct gccgcagagc tgcctattgg cgtggtcacc gcaacgttgg
    18481 gtgcgccggt gtttatctgg ttattgttaa aagcaggacg ttagccgcaa aaagacggtc
    18541 tatgattaaa agctaagatt tttactgacc acacccagga gaaacgatgc aagattccat
    18601 tctgacgacc gtagtgaaag atatcgacgg tgaagtgacc acgctggaga agtacgccgg
    18661 taatgtgctg ttgattgtca atgtcgcctc aaagtgtggc ttaacgccgc aatatgagca
    18721 gttggagaat attcagaaag cctgggccga tcgaggtttt gtggtgctgg gattcccgtg
    18781 caaccagttt ctggaacaag aaccgggcag cgatgaagag attaaaactt actgtaccac
    18841 cacatggggg gtgacgttcc cgatgttcag taagattgaa gttaacggcg aaggacgcca
    18901 tccgctgtat caaaaattga ttgccgcagc gccgaccgca gtcgcgccgg aagagagcgg
    18961 attctatgcc cgtatggtca gcaaaggtcg tgcaccgctg taccctgatg atattttatg
    19021 gaattttgag aaatttttag ttggcaggga cggattggtc atccagcgtt tttccccgga
    19081 tatgacgccg gaagacccca ttgtgatgga aagcattaaa ctggcgttgg caaaataatg
    19141 tctattgtga tgcagttaca ggatgttgcg gaatctaccc gcctggggcc actttctggc
    19201 gaggttcggg ctggggagat cctgcacctg gtggggccga atggcgcggg taagagtacc
    19261 ttactggcac gaatggccgg aatgaccagc ggtaagggaa gtattcagtt cgcggggcaa
    19321 ccactggaag catggtccgc aacaaaactc gcgctgcatc gcgcctatct ttcacaacag
    19381 cagacgccgc cgtttgcaat gccggtctgg cactatctga cactgcatca gcacgataaa
    19441 acgcgtaccg aactactgaa tgatgtcgca ggggcgctgg ctcttgatga caaactcgga
    19501 cgtagcacca atcaactttc cggcggtgaa tggcaacgcg tacgtcttgc tgcggtggtg
    19561 ctgcaaatca caccacaagc caatcccgca ggccaattgc tgcttcttga tgagccgatg
    19621 aacagtcttg atgttgcgca acaaagtgcg ttagacaaaa ttctgagcgc tctgtgtcag
    19681 caaggactgg cgattgtgat gagcagtcac gatctcaacc acacattgcg tcatgcgcat
    19741 cgggcgtggt tgctaaaagg tggaaaaatg ctggccagtg gacgcagaga agaggtgctc
    19801 acgccgccaa atctggcgca ggcctatggg atgaattttc gccgtctgga tatcgaaggt
    19861 cacagaatgc tgatttcgac catctgattt cattagtatc ttgaaaatcg ggtaattacg
    19921 acgctaaatt aatgcaagaa taaaaacaga ggaaccgtac ggaatgcgtt tctgccttat
    19981 tttgatcaca gcactgtttc tggccgggtg tagccaccat aaagcaccgc cgccaaatgc
    20041 cagactttct gattcgatta ccgttattgc cggtttgaac gaccagctac aaagctggca
    20101 tggcacgccg tatcgttatg gtggcatgac gcggcgcggt gtggactgtt cgggatttgt
    20161 ggttgtaacg atgcgcgatc gtttcgattt gcagctgccc cgagaaacca aagaacaagc
    20221 ctctatcggc acgcaaattg ataaagacga gttgctgcct ggtgacctgg tctttttcaa
    20281 aacgggttcc ggacaaaatg gtttgcatgt aggtatttac gataccaaca accaatttat
    20341 ccacgcctct accagcaagg gagtgatgcg ttcctcactt gataatgtct attggcagaa
    20401 aaatttctgg caggcgagac gaa
//
LOCUS       DAZOZY010000059        20328 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZOZY010000059 DAZOZY010000000
VERSION     DAZOZY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20328
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.059"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            212..712
                     /gene="tssB"
                     /locus_tag="ACHURQ_001883"
     CDS             212..712
                     /gene="tssB"
                     /locus_tag="ACHURQ_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR0823668.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            747..971
                     /locus_tag="ACHURQ_001884"
     CDS             747..971
                     /locus_tag="ACHURQ_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823669.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1022..2497
                     /gene="tssC"
                     /locus_tag="ACHURQ_001885"
                     /pseudo
     CDS             1022..2497
                     /gene="tssC"
                     /locus_tag="ACHURQ_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2504..2917
                     /gene="tssE"
                     /locus_tag="ACHURQ_001886"
     CDS             2504..2917
                     /gene="tssE"
                     /locus_tag="ACHURQ_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR0823670.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2921..4771
                     /gene="tssF"
                     /locus_tag="ACHURQ_001887"
     CDS             2921..4771
                     /gene="tssF"
                     /locus_tag="ACHURQ_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR0823671.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4735..5817
                     /gene="tssG"
                     /locus_tag="ACHURQ_001888"
     CDS             4735..5817
                     /gene="tssG"
                     /locus_tag="ACHURQ_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR0823672.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5842..7122
                     /gene="tagH"
                     /locus_tag="ACHURQ_001889"
     CDS             5842..7122
                     /gene="tagH"
                     /locus_tag="ACHURQ_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR0823673.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7119..7643
                     /gene="tssJ"
                     /locus_tag="ACHURQ_001890"
     CDS             7119..7643
                     /gene="tssJ"
                     /locus_tag="ACHURQ_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR0823674.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7646..8977
                     /gene="tssK"
                     /locus_tag="ACHURQ_001891"
     CDS             7646..8977
                     /gene="tssK"
                     /locus_tag="ACHURQ_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR0823675.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8982..9743
                     /gene="icmH"
                     /locus_tag="ACHURQ_001892"
     CDS             8982..9743
                     /gene="icmH"
                     /locus_tag="ACHURQ_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR0823676.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9752..12535
                     /gene="tssH"
                     /locus_tag="ACHURQ_001893"
                     /gene_synonym="clpV"
     CDS             9752..12535
                     /gene="tssH"
                     /locus_tag="ACHURQ_001893"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR0823677.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAEPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            12532..13275
                     /gene="vasI"
                     /locus_tag="ACHURQ_001894"
     CDS             12532..13275
                     /gene="vasI"
                     /locus_tag="ACHURQ_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR0823678.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13280..14692
                     /gene="tssA"
                     /locus_tag="ACHURQ_001895"
     CDS             13280..14692
                     /gene="tssA"
                     /locus_tag="ACHURQ_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR0823679.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14801..18235
                     /gene="tssM"
                     /locus_tag="ACHURQ_001896"
                     /pseudo
     CDS             14801..18235
                     /gene="tssM"
                     /locus_tag="ACHURQ_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18246..19695
                     /locus_tag="ACHURQ_001897"
                     /pseudo
     CDS             18246..19695
                     /locus_tag="ACHURQ_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19622..20104
                     /locus_tag="ACHURQ_001898"
     CDS             19622..20104
                     /locus_tag="ACHURQ_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0823680.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20148..>20328
                     /locus_tag="ACHURQ_001899"
     CDS             20148..>20328
                     /locus_tag="ACHURQ_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823681.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLT"
BASE COUNT         4803 a         5559 c         5613 g         4353 t
ORIGIN      
        1 gttcacacaa tctatttaac gcttgcagaa tacccctctt tacgatcgat aaaaagtcct
       61 tttcttattt attttgaagc gtaaaatgcg gggtgatgaa ggagtggtga ttttattttc
      121 accgccatta gggcggcatt gccgatattt ttttgcggta caaatatgcc gtgttcatgg
      181 cctgtacgag ataacatgac ggatcacttt tatgagcaaa aaatttgaag gcagcgtcgc
      241 gccccgcgag cgtattaata tatcgtatgt cccaaaaaca gatggccaga cggctgaagt
      301 ggaacttccg cttaacatgc tcgttgtcgg cgataccgga aatacgcagg aaacatcccc
      361 actggatgaa cgtcaggcag tttccgttaa caagcacaat ttcggtgcag tgatggctga
      421 agcagccatt ggcctcaatt ttactgtccc cgccacgctg aagggaagca caacagacga
      481 tgaactgaat gtggcgctga atatcaaatc actggatgat ttttcaccag atagcgtggc
      541 ccggcaggtt ccggaagtga ataaattgct ggaactgcgt gaagcactga ctgcactgaa
      601 ggggccgatg ggtaaccttc cggcattccg tacccagctc caggcattgc tggaaaatga
      661 agagtcccgc gagcagttgc tcaaagaaat cggtctggtg agcaataaat aaccaccact
      721 atttcattaa acagggttat ccaggcatga cggcacacaa aaatatctcc gggacatacc
      781 acctctctgt tgcagacatt ctgcaggtgg tttaccaggt atgtttcagc ccgtccgttg
      841 aaatcaacca ggatggtgtg gctgcgctga ttacaacact ggatcgccgt atcagtgatc
      901 tattggatga gattatccat ttttgtgagt ttcagcagag cgcgtcgcac tggcagcgcg
      961 tacttcactg atggcgaaaa gccggtttca gaaacacagc taaaacagaa ggattttcag
     1021 tatgtcttta caggaagagg aactcgtttc cagtcatgcc gggcagcctg agcaggcatc
     1081 gtcgctgctt gatcaaatca tggcacagac ccgtattcag cccggttcgg aaggttatga
     1141 cgttgccctc cagggtgtga cagcctttat tgccagcatc ctgcaatcca ctgcatctgc
     1201 cgaaccggtc aataaactgg ccgtggacag catgattgca gatattgatg aacgcatcag
     1261 ccgccagatg gatgtcatca tccatgcgcc tgcctttcag caggtcgagt cgttctggcg
     1321 ctcactgaaa accatggtgg atcgcgttga tttccgtgaa aacatcaagg tcaacgtgct
     1381 tcatgtcacc aagcaggaac ttctggaaga ctttgagttt gcgccagaaa ttattcagtc
     1441 cggattttat aagcacgttt attcgtccgg tttcggacag tttggcggta aacccatcgc
     1501 tgccgtgctg ggcgcttatg agttcaaaaa taccgcgcct gacatgaaac tgctgcaata
     1561 cgtcagtgcc gtgggcgcaa tggcgcacgc accgttcctg tcctccgttt ccccggagtt
     1621 tatggggctg aactcgtgga ccgaactgcc taatatcaaa gatctttatg ccatctttga
     1681 aggtccggcc tacaccaaat ggcgcgccct gcgtgactcg gaagattcgc gctatttagg
     1741 gcttacggca ccccgcttcc tgcttcgcca gccctattca ccaacagata accctgttaa
     1801 gaactttaac tattacgaag atgtcagcca gaaccacgaa gattatctgt ggggtaatac
     1861 ggcctggatg ctggcatgca atatcgcaga cagttttgcc aaataccgct gatgtccaaa
     1921 cattatcggt ccgcaaagcg gcggcgcagt gaaagatctg ccgatgcatc tgttcgaaac
     1981 gatggggcag attcaggcca aaatcccaac cgaggtgctg gtcaccgatc gccgcgaatt
     2041 tgaactggct gaagagggtt ttatcaccct taccatgcgt aaagactctg ataatgcagc
     2101 ctttttctct gcaaactcgg tacaaaaacc gaagcacttt ccgggaaaag atgcagaaac
     2161 caactataag ctgggtacgc agcttcctta tctcttcatc atcaatcggt tagcgcacta
     2221 catcaaggtg ttgcagcgtg aacaactggg gtcatggaaa gaaaggagcg acttagaaag
     2281 agagcttaat acctggatcc gtcagtatgt tgccgaccag gaaaacccgc ctgcagacgt
     2341 gcgcagccgc aaacctctgc gcgctgccaa agttgaggtt atggatgtag aaggcgaacc
     2401 cggctggtac caggttgcgc taagcgtgag acctcatttt aaatttatgg gggcaaattt
     2461 tgagctttcc ctggttggcc ggttagacag ggagtaacac accatgatac cggatcggga
     2521 aagaaccaca ggttctcttt ttgagcgtat ggaagcctct tcggccagaa accggcaggg
     2581 aggcagtatt cattcgctca ggcaatccat tcgccagaac ctacgaaaca ttcttaatac
     2641 ccggtccggt agttgccggg gtgcgcctga gctgggcatt gatgaaccgg aaggggctga
     2701 aaacttccgc gaatccatga gtcgggccat cgagcaatgt atagaacgct atgaaccccg
     2761 gatctcacac gctgaagtgc aggcagtggt ttcatccgcc tccagcccgc tggatatgac
     2821 gtttcatatt acagcctggg tgacatttaa cgaaacgcat gaggtgcttg aatttgatat
     2881 ggcaccgaat ggcagccagc attaccgggt ggactgacaa atggaatttg aagaacgcta
     2941 tttccgggaa gaactcgatt acctgcgcca gcttagcaag ctgctggcaa cggaaaaacc
     3001 ccatctggcc cgcttcctgg ccgaaaaaga tgcagatccg gatattgaac gcctgctgga
     3061 aggggtggct tttcttaccg gcaatctccg ccagaaaatt gaggatgaat tcccagaact
     3121 gacgcacggg cttattaaga tgctatggcc taattacctg cgtccggttc cggcaatgac
     3181 ccttattgaa tatacgccgg atatggataa gtcttctgta ccggtgttaa tcccccgtaa
     3241 tgagcagttt acaaccaacg ccggggaaat cagagttgat gaagtgctgc cctctgatgc
     3301 taaaaaggag gagccgcctc cctgtacctt caccctctgc cgggatatct ggctgctgcc
     3361 cgttcgcctg gagcagattg aaaaccgcag tacgacccgt aatggtgtta tcaacatcac
     3421 cttttcggtc gcaccgggaa cggacttccg cacgctggat ctgaacaaac ttcgcttctg
     3481 gctcggcaat gacgacaact atacccgtga ccagctttat ttatggttct gcgaatactt
     3541 gcagggtgcc gacctgactg tgggtgaaca gcatattcgc ctgcctgagt ttatgctaaa
     3601 agctgtcggt tttgagccgc aggatgccat gctgccctgg ccgaaaaacg tccacagcgg
     3661 ctaccggatc cttcaggagt atttctgtta ccccgatgcg tttctctttt ttgatctttg
     3721 tggttgtccg gctttgcctg acggattgca ggcggaattc tttaccctgc aactgcgttt
     3781 ttcgcgccct ttgcccgtgg acatccggct gcgccgcgat tccctgcgcc tgtattgcgc
     3841 acctgccatt aatttattta tccaccatgc agaagccatc acgctggaca accggcgggc
     3901 agactatccg ctggttccca gccgccatta cccacaacat tacgatgtat tttccgttaa
     3961 cagtgtggtg agccaggtcc aggatatgtt caggaaaaaa gatctggggc gtcctgtttc
     4021 gacgcaggcc gcgcgccagt ggccagcctt tgaaagtttc agccatcaga tagaatacag
     4081 ccggaagcgg gaagtggtgt actggcatca ccggaccaaa acatccctgt tccatcgcgg
     4141 ctttgatcat acccttgcct ttatacatgc tgatggcagt tatccgtcag acgaatctct
     4201 gctcagtaat gaagtggttt cggtatcgct gacctgtacc aaccgtgagc ttccgtcaca
     4261 aattcgttcc ggcgatatca ccggcacaac cggaaaaaat gcggctgttg cttcatttcg
     4321 caacattacc cgcccgacgc aaccactctg gccggtcatt gatggcagcc tgcactggcc
     4381 cctactctcc gccatgaacc tgaattatct gtcattactg gatacggacg cgctgaagca
     4441 ggtcatcgcc aactttgatc gccacgcaat ccatcatccg cagacggcac ggctgtcaca
     4501 acaaaagctg gatgccattg agcgtctgga gacccgcccc gttgatcgcc tgtttacggg
     4561 tattcccgtc cgggggctgg cttccacgct gtatctgcac ccggagccgt ttgtctgtga
     4621 aggggaaatg tatctgctcg gtacggtgct ttcgcatttt ctgtcgctgt acgccagcgt
     4681 taactcattc cacatgctga ccgttgtgaa cacagaaagc caggagacat ggaaatggac
     4741 ggaaagaatc gggcagcatc ctcttatctg agtccgggaa atcccccggc agataaagag
     4801 cagaatgatc cgctggcaca ggttttccat aatgcctgct cttacaattt ttttgcgatg
     4861 gcggagctgt tgcaccgcct ggcaaagggt gaaaagggga cgccagaatt atccctgcgc
     4921 gacgatccgg cacaggaaac cctgcgtttc agcgccgatg ccagccttgc cttcccgtgc
     4981 agtgatatca gtgcgctgaa acgggatacg tcaggggcat tcaggatgac caccaccttt
     5041 atggggttac aggggagcca gtcgcctctc cccggttatt acctcgctca cctggcctgg
     5101 aaagcggtac atgaacaaag tccggtaggt gattttctgg atatgttcag ccaccgcctg
     5161 acccagtttg tctggcatat ctggcgtaag taccgctatc acatcagttt tcgtaacggc
     5221 ggtgtggatg cgttctcgca gcgcatgtac tcccttgtcg ggctgggcca ccgccagctt
     5281 cgcgataagc tggcgattaa tcacagcaaa atgctggcgt attccgggat cctggcaaat
     5341 ccgggacgat ccccggaaat tatctgtggc ctggtgtccc actgctttga tttgtcagaa
     5401 gtgacactgc aaaactggca acgtcgcaag gtggatattg aaccggatca gcaaaatagt
     5461 ctggggagct attctcttaa aaatggcgaa aaactggcgg gaagatcggt actgggcaac
     5521 tttgttctgg gtacacgagt gcccgatctc agcggaaaat tccagctcag tattaccagc
     5581 ctgacccgaa aacagttcct ctcttttctg ccgtccggcg aaaacttcct gccactgacg
     5641 atgttcgtgt ccttcatcct gcgcgatcag cttgcctggg acttacatct ggggctggcg
     5701 ccggagcagg tgggcgcaat gcgcctgggc gataacaaaa gtgcgctgct gggctggacc
     5761 agcttcctcg gcacaccgga agaacgaccc tcagtcacca tcagggtacg gtcgtaaatt
     5821 atgataatca cggagcagaa tatgccggaa gagaaactac agacgctttc attgcaggtc
     5881 attaacggca gtgagctgga aagcgggcgg gcggcgcgct gtctgttcac acagcaggga
     5941 aatgtcggcc atggccccga atgccactgg tcggtacagg atcgtcagca gagcattccg
     6001 gcccaggctt ttaccgttat cctgcacgat ggcacatttt gtctgcgccc gcagacggca
     6061 caactgtggc tgaatcaggc aaaagtcaca gcaacatcag acctaataca gttgcgccag
     6121 ggcgatgaga tccagatcgg acggctgatg gtgagggttc atctgaaccg gggagatatt
     6181 ccccattacg atgaggaaat ggccactccc gaaaccatcg ttaccaatcg cgatatgctc
     6241 acggataccc tgctatcaac ggagggtgcg ccacactatc cgggaatgac tcaccggcac
     6301 cagcttgcag acaccgtggt aaacggtttt tctgccgatc cactccaggc tcttcagtcc
     6361 gaaagcctga ttaccacggg cgatccgctt tcaggcattg cggctgtccg gccatcggca
     6421 ccgctgtccg atccggcaag taatgggggg atcaatactc cgtttatgga tctgccgccc
     6481 atttatgcca gccccggcga tcgtaatgac gacgtctctg cggcagaaat ggcgcaacgc
     6541 cacgttgcgg tcaccccctt actgcgcggt cttggcggct cgcttaccat gagcaattcc
     6601 gacgatgcgg atgattttct ggaggaggcc ggacgaacgt tacaggccgc aataaaaggt
     6661 ctgctcgatt tgcagcagca gcgtaacagc ctctcagaca aacatttgcg cccgctggaa
     6721 gataacccgc tgcgcctgaa catggattac gccaccgcgc tcgacgtaat gtttgccgaa
     6781 ggtaaaagcc cggtacatct ggcggctccc gccgccgtca gtgaaagcct gcgcaatgtc
     6841 cgccaccacg aagaagctaa ccgggcagcg attgtggagt cgcttcgtgt cctgctggat
     6901 gctttctcac cacaaaatct gctgcgccgc tttgtgcagt accgccgcag ccatgaactg
     6961 cgccagccgc tggatgatgc cggagcatgg caaatgtaca gccattatta cgaagaactg
     7021 gcctccgatc gccagcaggg gtttgagatg ctgtttaacg aggtctacgc ccaggtctat
     7081 gaccgggtgc ttcgtgaaaa acagcgggag ccggaagcat gaacaggaag gcgtttctgg
     7141 cctgcgtgct gatgtgcgtc atattaaccg gctgcgaaac ggcgaaaaaa atcagtgagg
     7201 tgatcaaaaa cccggatatc caggtcggga gcctgaagga gcaaccttcg gaaataaccg
     7261 tcaccctgct gaccgaaccg gacaccaata ccaatgcgga aggtgaatca gccgccgtgg
     7321 atgtgcaact ggtgtacctg actgacgact caaaattgct ggcagccgac tatgaccaga
     7381 tagccagcac gccgctaccg gatgtgctgg gtaaaaacta tatcgaccac caggatttca
     7441 acctgttgcc ggacaccatc aaaaccctgc cgccggtaaa gctggatgag aaaacgcagt
     7501 ttattggtgt cgtggcctat ttctccgatg accaggccac tgaatggaaa caaatcgaga
     7561 cggtggaagg aatcggccac cattaccgcc tgctggtgca tgtacgtcaa agcagcatcg
     7621 aaatgaaaaa agaggatgaa taattatggc aaccacccgc aacaaggtga tgtggcagga
     7681 agggatgctg atgcgtccac accatttcca gcagcagcag cgttacaacg actacctgga
     7741 taaccagcgt ttccgggcca tgaatgattt atcctgggga tttaccgaac tcaccctcaa
     7801 caatgaactg ctggcgcagg gtaagatcat gattgacagc gcgtcaggca cactgcccga
     7861 cggcaccgtc ttttctatcc ccgaccagga cgcactgccc gatccgctgc acccgcaaaa
     7921 ttttccggac gaaagaagcc gcaatatcta cctcgctctg ccggtcgcca gtgatgtgag
     7981 aaatgaaatc agcgacggac ggcgaatcgg gcgttaccgg ctgaattatg ccgatgtccg
     8041 ggatttgcat tcagaagaag gtgacgcgcg aacattgacg ctgggtcaac tgacgccgcg
     8101 cattatgagc ggtgcagaag atatgagcgc ctatattacg ctcccgcttt gccgtatcag
     8161 cgatcgccat gccgatggct ccctgacgtt ggatgacgat tttatcccct cctgccagaa
     8221 tattcaggtc agtaagaaac tgcgtgttta tctcaaagag gtacaggggg ccattggcgg
     8281 acgggcaagc gatctggcaa accgtattgg ctctccggcg cagagcggca tcgcggatgt
     8341 ggcggaattt atgatgttgc agttacttaa ccgtaaccag acccggttta cccatcacgc
     8401 ccgtcgatcc cagctccacc cggaagattt ctaccttgat cttgccgggt tgctgggtga
     8461 actgatgacc tttaccgagc cgtcgcgcct gccctgcccg cttgatgtgt atgatcatca
     8521 tgacctgacc aaaatattta aaacgctgtt accggaagtc aaacgggcgc tgcataccgt
     8581 actgtcgcca agagccgtca atctgccgct gcatctgcgt gacggtatct ggcaggccga
     8641 tatccatgac acagaactgc tgcaatctgc cacctttgtg ctggccgtgg cggcaaatat
     8701 gccggtcgat cagatccagc gccagtttat ccagcagtcg aaaatttcct cgccggaaaa
     8761 aatccgcaat atggtcagtg tgcagatccc ctgtattcca ttgcgtgccc ttatggtggc
     8821 tccccgccag cttccttacc attccgggtt cagctatttc gaactcgaca agagtggcca
     8881 ggcctggaca gaaatggctg ccgccggggc cgttgcactg catgtttccg gcagtttccc
     8941 ggatctgaac atgcaactgt gggcgataag agggtaagac gatggatgag gggtcactgt
     9001 ctctgccacc atttaccgga cacgacgaaa aaagccagcg caactaccat ctcgcgcttc
     9061 gtggcaacag cctgaatccg atgatcgacg cggccacgcc gctactcggt atggtaatgc
     9121 gcctttcaac catgaacagc cagacgatgc cggagcatct ttttgctcag gtggtgacag
     9181 atgttcaggc cgtggagcaa ctgcttcagg agcagggcta cgaaccgggc gtgattatct
     9241 cgtttcgcta catcctctgc acgtttattg atgaagcggc gctcggtaac ggctggtcaa
     9301 acaagaacga gtggatcaag cagtcattgc tggtccattt ccacaacgaa gcctggggtg
     9361 gcgagaaagt tttcatcctg ctggagcgcc tgatccgtga acccaaacgt taccaggatc
     9421 tgctggaatt tctctggatc tgtttttctc tgggatttcg cgggcgctac aaagtggcag
     9481 cacaggatca gggggaattt gagcagatct accgccgtct gtaccacgtt ctgcataaac
     9541 tgcgcggcga tgcccctttc ccgctgttac accaggacaa aaaaacccag ggcgggcgtt
     9601 atcagctcat cagtcgcctg actgttaaac atattttttg tggtggcgtt gtcgtgctgg
     9661 cgttgtttta cctgttctat ctgttgcggc tggacagcca gacgcaggac atcctgcacc
     9721 agctaaacaa attacttcga taaggacgtt tatgatccag attgatcttc ccacgctggt
     9781 aaaacggctg aacctgttct cccgccaggc gctggagatg gccgcctctg aatgtatgag
     9841 tcagcaggca gcggaaatta ccgtcagcca tgtgctcatt cagatgctcg ccatgccacg
     9901 cagtgacctg cgggttatta cccgccaggg cgatattggc atggaagagt tgcgccaggc
     9961 gctgacggta gagaactaca caaccgcccg ttctgcggac agctacccgg cgttttcccc
    10021 gatgctggtt gagtggctta aagagggctg gctgctggcg tcggctgaga tgcagcacag
    10081 cgaactgcgc ggcggcgtgt tgctgctggc cctgctgcat tcgccgctgc gttatatacc
    10141 gcctgctgcc gcccggctgt tgaccggcat taaccgtgac cgtctgcaac aggactttgt
    10201 gcagtggaca caggagtcgg cggaatcagt cgtgccggat gcagacggta aaggcgcagg
    10261 cacaatgacg gacgcctctg acaccctgct tgcccgctat gccaaaaaca tgaccgcaga
    10321 cgcccgtaac ggcaggcttg acccggtact gtgccgcgac cacgaaatcg acctgatgat
    10381 cgacattctc tgccgccgcc gtaaaaacaa cccggtggtg gtgggcgaag cgggcgtggg
    10441 caaaagcgca ctgattgaag ggctggcgct gcgcatcgtg gcaggccagg tgccggacaa
    10501 gctgaaaaac accgatatca tgacccttga cttgggcgca ttgcaggccg gggcgtcggt
    10561 gaagggtgaa ttcgaaaaac gtttcaaagg gctgatggcg gaggtcattt cctccccggt
    10621 gccggtcatt ctgtttatcg acgaagcaca taccctgatt ggcgcgggca accagcaggg
    10681 cgggctggat atctccaacc tgctcaaacc ggcgctggcg cgcggcgagc tgaaaaccat
    10741 cgccgccacc acctggagcg agtacaaaaa atacttcgaa aaagatgccg ccctgtcgcg
    10801 ccgcttccag ttggtgaagg tcagcgaacc caacgctgcc gaagccacca ttattctgcg
    10861 cggtctgtcg gcggtctatg aacagtctca cggcgtgctg attgatgatg acgccttgca
    10921 ggccgctgcg acattaagcg agcgttatct ctccgggcgt cagttaccgg acaaagcgat
    10981 tgatgtgctg gataccgcct gcgcccgtgt ggccatcaac ctgtcgtcgc cgccgaagca
    11041 aatctcggcg ctgaccactc tgagccacca gcaggaggcg gaaattcgcc agcttgagcg
    11101 cgagcttcgc atcggactgc gtaccgacac atcacggatg accgaggtgc tggtgcagta
    11161 tgatgaaacg ctgacggcgc tggatgaact ggaagcggcc tggcaccagc agcagacgct
    11221 ggtccgggag attatcgcgc tgcgccagca gttactgggc gtggcagagg acgatgcggc
    11281 gccgttgccg gacgcagata ccgtggagga tacgcagcca gagtcagagt cagagtcaga
    11341 gtcagagtca gagcaggata ataccggtgc cgaaccggct gatgaggccg acagagaaca
    11401 accggaagag accgctgaaa cagtttcccc ggtacagcgg ctggcccagc tcactgccga
    11461 actggacgcc ctgcataacg accggttgct ggtctccccg cacgtcgata aaaaacagat
    11521 tgcggcggtg attgccgaat ggaccggcgt gccgctcaac cgcctgtcgc agaatgaaat
    11581 gtcggtcatc accgacctga cgaaatggct gggtgacacc atcaaaggcc aggacctggc
    11641 gattgccagc ctgcataagc atctactgac cgcacgcgcc gacctgcgtc gtccgggacg
    11701 cccgctcggt gcgttcctgc tggctggccc cagcggtgtg ggtaaaaccg aaaccgtcct
    11761 gcaactggca gagctgctct acggcggtcg ccagtacctg accaccatca atatgtccga
    11821 attccaggag aaacacaccg tctcgcggct gattggttcg cctccgggct acgttggcta
    11881 cggtgaaggc ggcgtactga ccgaagcgat tcgccagaaa ccctactcgg tagtactgct
    11941 cgatgaagtg gaaaaagcgc acccggatgt gctcaacctg ttctaccagg cgttcgataa
    12001 gggcgaaatg gcagacggtg aaggccgcct gattgactgc aaaaatatcg tcttcttcct
    12061 gacgtccaac ctcggctacc aggtaatagt cgagcatgcc gatgacccgg aaaccatgca
    12121 ggaagtactg tatccggtgc tggccgactt cttcaaacct gccctgctgg cgcgtatgga
    12181 agtggtgccg tatctgccgc tgtcgaaaga gacgctcgcc accattatcg ccggaaaact
    12241 ggcccgtctg gataacgtgc tgcgcagtcg ctttggtgta gaagtggtca ttgaaccgga
    12301 agtgacggac gaaatcatga gccgcgtcac ccgcgcggaa aacggcgcga ggatgctgga
    12361 atcggtcatc gatggcgaca tgctaccgcc gctctcgctg ctgctgttgc agaaaatggc
    12421 ggctaacacg gcgattgccc ggattcggtt gtcggcagtg gacggcgcat ttacggcaga
    12481 cgtggaagat gctcagaacg acgagtccgt cacaaaggat gaaacggttt tatgagcagg
    12541 ttgcgcgttt tccggcagac aggatggttt atcgcgggcc tgatgacggg cttacccgcc
    12601 actgccgcgc ccgcagaagc atcgtcaatg gccggtgtgg cggtcgccgt cgccaccacg
    12661 accccgccgg atgcgacagc cacgttacag gccatgcagt cctgtcggcg ggagtccgcg
    12721 gcgctggagc ggcttgactg ctatgaccgc ctgctggcac cgctgtcccc gtcagggttt
    12781 gacggggcgc tggtcaaagc cggttttgtg ggcgaggcct ggacccgcgc cacagagcag
    12841 gaaaaacgcc gcgagggcaa caccacagaa ctgctggtga cgcaggtgcc gggggagcgc
    12901 ccgacggtgg tgataaccac cccggctatc ggtcatgttc cgccacgccc ggtgctgatg
    12961 ttcagttgcg tggacaatat cacccggatg caggtggcgc tgatgcaccc tctggatgtc
    13021 catgatatcg ccgtcactct gaatgccgac aaccgggcat tgcgcagcca ctggtttgtg
    13081 cgcgagaacg gcaccctgct ggagtccagc cgggggttat ccgggattga tgaaattaag
    13141 caactgttcg gcgcaaaaac gctgacggtg gataccggcg cagacaatgc cgccggaaag
    13201 ctgaccttta acattgacgg gctggcaagg gcgattgctc ccctgcgcga tgcctgccac
    13261 tgggcgggag aataaaaata tggcaatgga cttacgcgat ccgaatgtct ggatatcgca
    13321 cctgctggaa aacctgccgg aagagaaact ggcatcggcg ctgaaagatg acaacccgaa
    13381 ctgggagtat atcgacggcg aaatcgtcaa gctgggatcg ctggcccaca gtcaactgga
    13441 tatcccggaa ctccagcgcc gggggctggt cattctggct tcggagagta aggattttcg
    13501 tctgctggcg catctgctaa gaaccctgca acacgccggt gatcctcatc tcgcattgcg
    13561 cctgctggcg ctgtatgtgg agcattactg ggcggtggct gcgccgcaga atatggcgca
    13621 taaaaaacgc tttgccagcc aggtcattaa acgctttgaa acaggtattg aagttttttc
    13681 acagaacgcc gccacagcgc agcgcgatgc gctgtcaggg caactggcaa aactggcgca
    13741 atgctggcag tcacataacg ccccggaact ggcacaggct actgatgatc tttttgccct
    13801 gtaccagcgt gcgtttaatc gtgcggcacc ggctcctgtc cccactccgg cggcctccgg
    13861 tagttcacca cagaccaccg ccacgtctga aagcggcgtg acgcaaccca gtgctccggc
    13921 tccccaaatc gtcatcgaca gtcacgacga caaagcctgg cgcgacacgc tgttaaaagt
    13981 ggcggccatt ttatgtgaac gccagccgga ttcaccgcag ggctaccgtc tgcgccgcca
    14041 tgcgctttgg cagaacatca ccagtacgcc ccaggcggaa agcgatggcc gtactccact
    14101 ggctgcggtc tccgccgata tggtggcaga ttaccatgct cagctcggta gtgccgatat
    14161 ggcgctgtgg cagcaggtgg agaaaagcgt actgctggct ccttactggc tggatggtca
    14221 ctgcctttct gcacagacgg cgctgcgtct gggttacaaa caggtggcag acgccatccg
    14281 cgatgaggtc atccgcttcc ttgagcgtct gccccagctt accgggctgc tgtttaatga
    14341 ccacacaccg tttatcagtg agcagacgaa acaatggctg gcagcttcgc ccgacgccaa
    14401 agttgcaccg gttgcgcaaa tcggtgagga atcgaaggca gccagagcct gttttgctga
    14461 gcagggtctg gaggcggcgc tgcgatatct ggacatgcta cccgaaggcg atccccgcga
    14521 tcagtttcac cgccagtacc ttgccgcaca gttgacggaa gaggcggggc tggtacagct
    14581 tgcgcagcag cagtaccgga tgttgttcag gatgggattg cagatgatgg tggcagactg
    14641 ggagccgtcc ctgcttgagc agctagaaca aaaattcacg gcagaacaat aaaagagaac
    14701 aggagttttt gtgttcagat taccaacacc ccgactactc agcggactca aatcagccct
    14761 gcgaccggcg atgccccggt ttaaagtctc tgctttctgg ctgctgatac tggcgtggat
    14821 ttttctgctg gtgtggatct ggtggaaagg cccgatgtgg acgctgtatg aagaacagtg
    14881 gctcaaacca ctggcgaacc gctggctggc aacggcggcg tgggggatta ttgccctggt
    14941 gtggcttacc gtccgggtga tgaagcgcct gcaacagctt gaaaaaatgc aaaagcaaca
    15001 gcgcgaggaa gccgttgatc cgctcagcgt ggaactgaac gcccagcagc gttatcttga
    15061 ccgctggctg ctgcgcctgc aacgccatct cgacaaccgc cgtttcctgt ggcagttgcc
    15121 gtggtatatg gtcatcggcc cggcgggcag tggcaaaacg acactgctgc gcaaggggtt
    15181 tccgtccgac attatttatg ccccggaggg cgcacgcggc gcagaacaac gcctgtacct
    15241 cacgccccat gtcggtaaac aggcggtgat ctttgatatc gacggcacac tctgcgctcc
    15301 cgctgatgcg gatatcctgc atcgccgtct gtgggaacat gccctcggct ggctgaaaga
    15361 aaagcgcgcg cgccagccgc tgaacgggat tattctgaca ctcgatttac ccgatctgct
    15421 gaccgcagac aaacgccgcc gcgagcatct gttacagacg ctgcgcagcc gcttacagga
    15481 tatacgccag catcttcact gccagttacc ggtttacgtg gtactgaccc ggcttgattt
    15541 attacagggt tttgccgccc tgttccagtc actcaacaga caggatcgcg atgcgattct
    15601 gggcgtcacg ttcacccgcc gtgcccatga aaatgatgac tggcgaacag agttgaatgc
    15661 tttctggcag acatgggtgg atcgaatgaa tctggcgttg ccggatctga tggtcgctca
    15721 gactcacacc cgcgcgtctt tattcagttt ttcccgccag atgcagggaa gccgtgaacc
    15781 gctggtgtca ctgcttgagg gtctgcttga tggcgaaaat atgaacgtga tgctgcgtgg
    15841 tgtctatctc acctcttcgc ttcagcgtgg acagatggat gatatattca cccagtctgc
    15901 cgcccgccag taccggctgg gcaataaccc actggcgtcc tggcccctgg tggacaccgc
    15961 gccttatttc acccgcagcc tgttcccgca ggcattactc gcagagccta atctggcaac
    16021 agagagccgc gcctggctga tacgttcccg tcgccgcctg acggttttct ctgccacagg
    16081 cggcgtggca gcactgctgc tcatcaccgg ctggcatcac tattacaacg gtaactatca
    16141 gtccggcatc accgtgctta agcaggccaa agcctttatg gacgtgccgc ctccgcaggg
    16201 ggaagatgac tttggcaacc tgcaactgcc gctcctgaac ccggtacgcg atgccacact
    16261 ggcctatggc gactggggcg accgcagccg tctggccgat atgggactgt accagggacg
    16321 acgtatcggg ccttatgtgg aacagaccta tctgcaactg ctggagcaac gttacctgcc
    16381 ctcgctgttt aacgggctgg tcaaagcgat gaacgccgcg ccgccggaga gtgaagaaaa
    16441 actcgcggtg ctgcgcgtga tgcgaatgct ggaggacaaa agcggacgta acaatcaggt
    16501 ggtgaagcag tatatggcaa aacgctggag cgaaaagttc cacggtcagc gcgatatcca
    16561 ggcacaactg atgtcccatc ttgactacgc gctggctcat actgactggc acgcagagcg
    16621 tcaggcgggc gacggtgacg ccatcagccg ctggacgcca tatgacaagc ccgtggtatc
    16681 agcacagaaa gaactgagca aactgcctgt ctaccagcgg gtttaccaga gcctgaaaac
    16741 gcgggcgctg ggcgttcttc ctgccgacct caatctgcgt gaccaggtag ggccaacctt
    16801 tgaccaggtg tttacatctg ccgatgacaa caaactggtt gttccacagt ttatcacccg
    16861 ctacggcctg caaagctatt ttgtaaaaca gcgcgatgaa ctggttgaac tgacagcgat
    16921 ggattcctgg gtacttaacc tcacccgcaa tgtgaaatac agtgacgccg accgtgcgga
    16981 aatccagcac cagttgaccg agcagtatat cagcgactac accgccacct ggcgggctgg
    17041 gatggacaat ctgaatatcc gcaattttga gtccatcgga caactgaccg gggcgctgga
    17101 gcaggttatc agcggcgacc agcctttgca gcgggcgctg accgtgctgc gtgacagcac
    17161 acagccaggc gtcttttctg aaaaactctc tgccaaagaa cgggaggaag ccctggcaga
    17221 gccggattac cagttactca cccgcctcgg gcatgaattc gccccggaaa acagtaccct
    17281 ggcagtacag aaagacaaag aaagcacgat gcaggccgtg tatcagcaac tcaccgagtt
    17341 gcaccgctac ctgctggcaa tccagaacgc gcctgtacca gggaaatcgg cgctgaaagc
    17401 cgtgcagtta cggcttgatc agaacagcag cgatccgata ttcgccaccc gccagatggc
    17461 aaaaacgctg cctgctccgc tcaaccgctg ggttggcaga ctgactgact aggcctggca
    17521 tgtggtgatg gtggaggctg ttcattatat ggaagtggac tggcgcgaca gcgtggtgaa
    17581 accgtttaac gagcaactgg caaataacta tccgtttaat ccgcgttctg cacaggatgc
    17641 ctcactggat gccttcgaac gcttctttaa accggatggc atactggata ccttctacca
    17701 gcagaacctg aagctgttta tcgataatga cctgagtctg gaggatggcg ataacaacgt
    17761 cattattcgc gaagatatta ttgcgcaact ggaaactgcg cagaaaatcc gtgacatctt
    17821 cttcagcaaa cagaacggtc tgggaacatc ctttgccgtg gaaacggtat cgctttcagg
    17881 caataaacgc cgcagtgtac tgaaccttga cagtcagtta gtcgattaca gccagggccg
    17941 taactatacc gcccatctgg tctggcctaa caacatgcgc gaaggcaacg aaagtaagct
    18001 gacgctcatc ggcaccagcg gcaacgcgcc gcgcagtatc agcttcagcg ggccgtgggc
    18061 gcagttccgc ctgttctggg ccggacaact gaccggagta caggatggca actttaccgt
    18121 gcgctttagc gtggacggtg gcgcgatgac ctaccgtgtg cataccgaca cggaagataa
    18181 cccgttcagc ggtgggttgt tcagccagtt tggtctgtca gacacactgt actgaggagg
    18241 cagggatgaa tagtaacgta ctgacacaaa ctatcgttac cggcagtgac ccgcgcgggc
    18301 tgccggaatt cagcgccatc cgcgaggaaa taaacaaagc cagccacccg tcacagcctg
    18361 agctgaactg gaaactggtg gagtcgctgg cgctggcgat ttttaaagcc aacggtgtgg
    18421 atttacacac cgccacctac tatacgcttg cccggacacg gacacaggga ctggcgggat
    18481 tctgcgaagg tgcggaactg ctggcggcaa tggtaagcca cgactgggat aagttctggc
    18541 cgcagggcgg cccggcgcgt actgaaatgc tggactggtt taactcccga accggcaata
    18601 ttctgcgtca gcaaatctcc tttgcggaat ccgacctacc actgatctac cgcacagagc
    18661 gggcattgca gcttatctgc gataagctcc agcaggtgga actgaagcgc gttccgcgcg
    18721 tggagaatct gctctatttt atgcagcaca cgcgtaaacg gcttgaaccc cagccgaaga
    18781 gtaacgctga gaacgccgca cagaccacgg tcagaacgct gatttatgcc ccggaaacac
    18841 tggcatcttc cacaccagaa gcggtagtgc ctcccctgcc cggcctgcct gagatgaaag
    18901 tggaagtgcg cagtctgaca gagaatcccc cacaggccag tgtgataaag caaggcagta
    18961 cggtaagagg gtttatcgca gggatcgcct gttcagtggc tgtcgcctca gcattgtggt
    19021 ggtggcaggt ctatccggtg cagcagcaac tgttacaggt taacgacacc gctcagggcg
    19081 cagcaacggt gtggatggcc tcacctgaac tcgaaaacta tgagcgcagg ctgcaacaac
    19141 ttcttgatac ctccccggta cagccgctgg aaaccgggat gcagatgatg cgtgttgccg
    19201 acagtcgctg gccggaaagc ctgcaacagc aacaggcctc gacacaatgg aatgaggcac
    19261 tcaaaacccg cgcacagagt agcccgcagt tgcgtggctg gttgcagacc cgccaggact
    19321 tacatgcttt tgcagatcta gtgatgcagc gcgagaaaga gggactaacc ctttcctata
    19381 tcaaaaatgt catctggcag gcggagcggg gactggggca ggaaaccccc gttgagtctc
    19441 tgttgacgca gtaccatgat gcccgtgcgc agaagcagaa tacagatgcg ctggaaaaac
    19501 aaattaatga gcgactcgaa ggcgtgttaa gccgctggct gctgctgaag aataacgtca
    19561 tgccagaggc ggcaaccggt accacggccg aaaaataatt atttaattaa aggagattat
    19621 catggcaaat ataagttatt tatctttgag tggagagaca caagggctaa tttcagcagg
    19681 ttgctcaacg cttgattctg tcggaaacaa agcgcagcca gaacataaag atcagatcat
    19741 ggtttatgcg ttaatgcatt ccatctcaag aagtcaaaat gtcaatcatc atgaattaat
    19801 tatcaccaaa ccggttgata aatcctcacc attgctggca aaagcactta gtgacaatga
    19861 aaaaatggca atatgtgaat ttatactcta ccgaaccagt aaagcgggta tttaccagcc
    19921 ttattataaa ataaatctgt caaaagcccg aatatcaagt attgattttg tcacaccgca
    19981 tgctgtcctg gaaaaagaac tggaaccaca ggagagaatt gcctttattt atgaagatat
    20041 ttcctgggag catacgttag ccggtacgaa tgcaatgagt aaatggcagg atcgtgtaca
    20101 gtaacttact gtaataacat aataaattac tattgcggag agataaaatg gctggtttaa
    20161 ggaataacaa caacacacag aatgcacagt gggctgatta tgtgggtgac attcttcggg
    20221 gggctcagcc gataaaccag ctagtacctc agcaccctta tcttaacgac gtcccactca
    20281 ttgatgagtt aaggcatcag aacactcatc atgttattct cttgacac
//
LOCUS       DAZOZY010000060        20246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZOZY010000060 DAZOZY010000000
VERSION     DAZOZY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.060"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            319..990
                     /locus_tag="ACHURQ_001900"
     CDS             319..990
                     /locus_tag="ACHURQ_001900"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR0823682.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1000..2196
                     /locus_tag="ACHURQ_001901"
     CDS             1000..2196
                     /locus_tag="ACHURQ_001901"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0823683.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2196..2756
                     /locus_tag="ACHURQ_001902"
     CDS             2196..2756
                     /locus_tag="ACHURQ_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0823684.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2758..3531
                     /locus_tag="ACHURQ_001903"
     CDS             2758..3531
                     /locus_tag="ACHURQ_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR0823685.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3719..3994
                     /gene="frmR"
                     /locus_tag="ACHURQ_001904"
     CDS             3719..3994
                     /gene="frmR"
                     /locus_tag="ACHURQ_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR0823686.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4029..5138
                     /gene="frmA"
                     /locus_tag="ACHURQ_001905"
     CDS             4029..5138
                     /gene="frmA"
                     /locus_tag="ACHURQ_001905"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR0823687.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5231..6064
                     /gene="fghA"
                     /locus_tag="ACHURQ_001906"
     CDS             5231..6064
                     /gene="fghA"
                     /locus_tag="ACHURQ_001906"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0823688.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6290..6829)
                     /locus_tag="ACHURQ_001907"
     CDS             complement(6290..6829)
                     /locus_tag="ACHURQ_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR0823689.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6931..8142)
                     /gene="mhpT"
                     /locus_tag="ACHURQ_001908"
     CDS             complement(6931..8142)
                     /gene="mhpT"
                     /locus_tag="ACHURQ_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR0823690.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8429..9442)
                     /gene="mhpE"
                     /locus_tag="ACHURQ_001909"
     CDS             complement(8429..9442)
                     /gene="mhpE"
                     /locus_tag="ACHURQ_001909"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR0823691.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9439..10389)
                     /gene="mhpF"
                     /locus_tag="ACHURQ_001910"
     CDS             complement(9439..10389)
                     /gene="mhpF"
                     /locus_tag="ACHURQ_001910"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR0823692.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10386..11195)
                     /gene="mhpD"
                     /locus_tag="ACHURQ_001911"
     CDS             complement(10386..11195)
                     /gene="mhpD"
                     /locus_tag="ACHURQ_001911"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR0823693.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11205..12071)
                     /gene="mhpC"
                     /locus_tag="ACHURQ_001912"
     CDS             complement(11205..12071)
                     /gene="mhpC"
                     /locus_tag="ACHURQ_001912"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR0823694.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(12089..13033)
                     /gene="mhpB"
                     /locus_tag="ACHURQ_001913"
     CDS             complement(12089..13033)
                     /gene="mhpB"
                     /locus_tag="ACHURQ_001913"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR0823695.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(13035..14699)
                     /locus_tag="ACHURQ_001914"
     CDS             complement(13035..14699)
                     /locus_tag="ACHURQ_001914"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR0823696.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14741..15574
                     /locus_tag="ACHURQ_001915"
                     /pseudo
     CDS             14741..15574
                     /locus_tag="ACHURQ_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15651..16733
                     /gene="lacI"
                     /locus_tag="ACHURQ_001916"
     CDS             15651..16733
                     /gene="lacI"
                     /locus_tag="ACHURQ_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR0823697.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16856..19930
                     /gene="lacZ"
                     /locus_tag="ACHURQ_001917"
     CDS             16856..19930
                     /gene="lacZ"
                     /locus_tag="ACHURQ_001917"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR0823698.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            19982..>20246
                     /locus_tag="ACHURQ_001918"
     CDS             19982..>20246
                     /locus_tag="ACHURQ_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0823699.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         4792 a         5537 c         5231 g         4686 t
ORIGIN      
        1 gcatattaca ttcacataat gtatataaac aatacttgtt cacagaacat attttttaaa
       61 tgcaattaat tgacaaacta aatcacatct aaatacaatg tataaaacaa aataactata
      121 cataccaaac atacaaacac ttaacatatg aattgcataa aaacattacc cagtcgaacc
      181 tccgggtctg aaagcccttg cttttcagtt attagcaaca tttaacattt ataaattaac
      241 atctggctgc gccagagatt acgtctctga gtgcatcgtt gtgcttgtag tataataaat
      301 aaaatggagt gtcattaagt ggataaggtt ttagattcag ccctcctttc ttcggcaaat
      361 aaaagaaagg gtatccttgc tattggcgca catcctgacg atatagaatt aggctgtggc
      421 gcatcgcttg ctcgtcttgc gcaaaaagga atttatatcg cagcagtggt gatgactacc
      481 ggcaactctg gtacagatgg aataatcgat cgccatgaag aatcgcgcaa cgccctaaag
      541 atattagggt gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag
      601 ctcaatgata tgatttccgc cctggaagac atcattaaaa atcaaattcc ttctgatgtt
      661 gaaatcatgc gggtatatac catgcatgat gccgaccgcc atcaggatca tctcgctgtt
      721 tatcaagctt caatggttgc ctgccgcact attccacaaa ttctcggcta cgaaacccca
      781 agtacctggc tttcatttat gcctcaggtt tttgaatccg ttaaagaaga atatttcaca
      841 gtcaaacttg ctgcattaaa aaaacataaa agccaggaac gacgtgatta tatgcgtcat
      901 gatcgcctgc gtgcagttgc acaatttcgc gggcaacagg tcaatagcga tctgggtgaa
      961 ggctttgtaa ttcataaaat gattctttga gcaaattgca tgaaaacctg gatatttatc
     1021 tgtatgtcca tagcaatgtt gctatggttt ttaagtacgc taagacgtaa acccagtcaa
     1081 aagaaaggct gtattgacgc cattatacct gcgtataacg aaggcccgtg tctggcgcag
     1141 tcactggata atctactgcg taacccttat ttttgccggg taatttgcgt taacgacggc
     1201 tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt
     1261 gttgccgtca cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat
     1321 gccacctgcg accaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac
     1381 ggaatgggct atatgctggc agaaattaag cgcggtgctg atgccgtagg cggcattccc
     1441 tctactgcgt tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg
     1501 atgattgtta tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt tattatcagc
     1561 ggtgcctgcg gaatgttccg tactgatgta ttgcgtaagt tcggtttctc ggatcgtact
     1621 aaagtcgaag accttgatct cacctggaca ttagtggcaa acggctaccg tattcggcag
     1681 gcgaatcgct gcatcgtata cccacaggaa tgcaacagtc cgcgtgagga gtggcgtcgc
     1741 tggcggcgtt ggattgtggg ctacgcggtc tgtatgcgcc tgcataaaag gcttttattt
     1801 agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgattta tggcgttggg
     1861 atttatctca ctacctggtt taatgaattc atcaccaccg ggccgcatgg tgtggtgttg
     1921 gcaatgtttc cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt tagcgcctgg
     1981 tttcatcgtt gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta tgtattatta
     2041 gcttatgcca tctggattat ctatggactt attgcctttt ttactggacg cgaacctcag
     2101 cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaacct
     2161 tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa attattcaga
     2221 ttgcagacca ggtaattatt gatgaaactg caggtgaagt tgttatcggc gcgaataccc
     2281 gtatttgtca tggtgccgtt attcagggtc cggtagtgat tggcgcaaac tgcctgatag
     2341 gtaattatgc gtttattcgt cctggcacaa taatcagcaa tggtgtaaaa attggttttg
     2401 ccaccgaaat taaaaatgcg gttattgaag cggaagcaac gattggtccg caatgttata
     2461 ttgccgactc ggtagttgca aaccaggcat atttgggcgc acaagtacgt accagtaatc
     2521 atcgtctgga tgaacagcct gtgtctgttc gaactccaga gggaattatc gctaccggat
     2581 gcgataaatt aggttgttat atcgggcagc gttcacgcct tggcgtacaa gttattattt
     2641 tgcctgggcg aattatttcc ccgaacacgc aacttggccc gcgcgtgatt gtagaacgta
     2701 atttacctac cggaacttat tcactccgac aagaacttat ccgtacagga gattaaaatg
     2761 ataaaacgga cgttattagc ggcggccatt tttagcgcat tgcccgctta tgccgggtta
     2821 acttccatta ccgcgggcta cgattttacc gattattctg gcgatcatgg caaccgtaat
     2881 ttagcgtatg ctgaactggt ggcgaaagtt gaaaacgcaa cgctgctttt taatctttcc
     2941 caggggcgtc gtgattatga aactgaacat ttcaatgcca ctcgcggtca gggtgcggtc
     3001 tggtataaat ggaataactg gctgacaacc cgaacgggta ttgcctttgc ggataatacg
     3061 ccggtctttg cccgccagga ttttcgtcag gatattaacc tggccctgtt gccaaaaacg
     3121 cttttcacga ccggttatcg ctacactaaa tattacgatg atgtcgaagt cgatgcctgg
     3181 caaggcggcg tatcactcta tactggcccg gtgatcacca gctaccgcta tacccattat
     3241 gactccagcg atgcaggtgg tagttatagc aatatgattt ccgtgcgtct gaatgacccg
     3301 cgcggcactg gttatacgca actatggcta agccgcggaa caggcgctta cacctatgac
     3361 tggacgccag aaacacgcta cggcagcatg aagagcgtca gtttgcaacg tattcaaccg
     3421 ctgactgagc aacttaatct cgggctgacg gcaggtaaag tgtggtacga caccccaacc
     3481 gatgattata acggtctgca acttgcagcc catctgacct ggaaattctg attgcttctg
     3541 ccgcccgcta tccggggcgg ccttccctgc cgattagccc cactcccttt cctcgttgtt
     3601 ttccgaccac attcaccgga taaattttat tctccagcgt tatatactat agggaggtat
     3661 gcattgacat atagcatacc tccctatagt atattgcgtg cagatgatga ggtgcgaaat
     3721 gcccagtact ccggaagaga agaaaaaggt ccttactcga gttcgtcgta ttcgggggca
     3781 gattgatgct ctggaacggt cgctggaggg tgatgccgaa tgccgtgcca tactccaaca
     3841 gatcgctgcc gttcggggcg cggctaacgg gctgatggca gaagtgcttg aaagccatat
     3901 ccgggaaacg tttgaccgaa atgactgcta cagccgcgaa gtcagccaat ccgttgacga
     3961 cactattgaa ctggttcgag cctatcttaa atagctgaat ctattaccat attgaggaag
     4021 agcgagagat gaaatcacgt gctgccgttg catttgctcc cggtaaaccg ctggaaatcg
     4081 ttgaaattga cgttgcacca ccgaaaaaag gtgaagtgct gattaaagtc acccataccg
     4141 gcgtttgcca taccgacgca tttaccctct ccggggatga cccggaaggt gtattcccgg
     4201 tggttctcgg tcacgaaggg gccggcgttg tggttgaagt cggtgaaggc gtaaccagcg
     4261 tcaaacctgg cgaccatgtg atcccgcttt acaccgcgga gtgcggcgag tgtgagttct
     4321 gtcgttctgg caaaactaac ctctgtgttg cggttcgcga aacccagggt aaaggcttga
     4381 tgccagacgg caccacccgt ttttcttaca acgggcagcc gctttatcac tacatggggt
     4441 gctctacatt cagtgaatac accgtggtcg cggaagtgtc tctggccaaa attaatccag
     4501 aagcaaacca tgaacacgtc tgcctgctgg gctgtggcgt gaccaccggt attggcgcgg
     4561 tgcacaacac agctaaagtc cagccaggtg attctgttgc cgtgtttggt cttggcgcga
     4621 ttggtctggc agtggttcag ggcgcgcgtc aggcgaaagc gggacggatt atcgctatcg
     4681 ataccaaccc gaagaaattc gatctggcgc gtcgcttcgg tgctaccgac tgcattaacc
     4741 cgaatgacta cgacaaaccg attaaagatg tcctgctgga tatcaacaaa tggggtatcg
     4801 accatacctt tgaatgcatc ggtaacgtca acgtgatgcg tgcggcgctg gaaagtgcgc
     4861 accgcggctg gggtcagtcg gtgatcatcg gggtggcagg ttctggtcag gaaatctcca
     4921 cccgtccatt ccagttggtc actggtcgcg tatggaaagg ttccgcgttt ggcggcgtga
     4981 aaggtcgttc ccagttaccg ggtatggttg aagatgcgat gaaaggtgat atcgatctgg
     5041 aaccgtttgt cacgcatacc atgagccttg atgaaattaa tgacgccttc gacctgatgc
     5101 atgaaggcaa atccattcga accgtaattc gttactgatt tcccgcaggt ataccccgtc
     5161 cacttcagac ggggttctta atactctccc cgggcagccg tccgggggat taaccgtgag
     5221 ataatgactg atggaactca ttgaaaaaca tgccagcttt ggcggctggc aaaatgtgta
     5281 tcggcattat tcccaatcac tgaaatgtga aatgaatgtc ggcgtctatc tcccaccaaa
     5341 agccgcgaat gaaaaattgc cggtgctgta ctggctttca ggcctgacct gcaacgagca
     5401 gaatttcatt actaaatcgg ggatgcagcg ttacgcggct gagcacaaca ttattgttgt
     5461 tgcgccggac accagtccgc gaggcagtca tgtcgcagat gccgaccgtt acgatctcgg
     5521 gcaaggtgcc gggttttacc tgaacgcaac gcaagcgccg tggaatgaac attacaaaat
     5581 gtatgactat atccgcaacg agctgccgga tttagtgatg caacattttc cggcaacgac
     5641 cagaaagtct atctctggtc attctatggg cgggctgggc gcgctggtgc tggcgttacg
     5701 taacccagat gaatatgtca gcgtctcggc gttttcgccc attgtctccc catcgcaagt
     5761 accgtgggga cagcaagcct ttgccgcata tcttggtgaa aataaagatg cctggttgga
     5821 ttacgatccg gtgagtctta tttcacaagg tcaacgcgtt gcggaaatca tggttgatca
     5881 ggggttgagt gatgattttt acgcggaaca gctgcggact ccaaatcttg aaaagatctg
     5941 ccaggagatg aatatcaaga cgttaatccg ttatcacgag ggttacgacc acagctatta
     6001 ttttgtctcc agttttattg gcgagcatat tgcctaccac gccaataaac tgaatatgcg
     6061 ttgataatag tgcacgactg ccggatgcgg cgtgaacgcc ttatccggcc tacacttcgc
     6121 ccgtaaaccg taggcctgat aagacgcgca aagcgtcgca tcaggcatgg cgcacgactg
     6181 ccggatgcgg cgtgaacgcc ttatccggtc tacacttcgc ccataaaccg taggcctgat
     6241 aagacgcgca tagcgtcgca tcaggcatct gcgcacgact tatctatgtt taccacatca
     6301 aatcatcagg cactttgaag tcggcatacg gatcgtcttc atcctgctct tcggcactga
     6361 gcgcgctgtg caacacaata ctgctggcat cacgctgagc aattttatcg gcaacactgg
     6421 cgggaataat ggtgtattca ccttcgctat tgttatcgac caacaagcgg gcaatcgcca
     6481 gacgaccatt aatcaactgc gcctgtgtga gcttatcgac aaaaatcttc ttaatcagat
     6541 tgccgtcagt gaagttaaaa ccaatatcgc cattggcaat ggtgattcgg ttcatttcaa
     6601 tgagctgctt cacctgggct ttatattctt tcgccaacgc cgcttgtttt tgctgttcgc
     6661 taagctgttt atcacgctca agctgtgcct ttttattttc ttctaccgca gcccgagctt
     6721 cacgcgcctg aacgcgcgat tttttcgccg ttctctcgac cttcgccgct tttttgctgg
     6781 tgactaatcc tgctttgagc aactgctctt gtaaggtaag ttttgccatt tttgtttcca
     6841 aacccgctga ataattttta gcgagtatac ctgtaaacgc cgtgggtgta ttgggggcaa
     6901 gtccctttcg cccctgctta ataaggcgcg tcaggcatcg gcacacggct gcattcttga
     6961 tctccgactc atcaaaataa acaccgccaa cccagcaaca agaatacccg gtgcagaagc
     7021 ggccattacg ccgaccgtgc cagtgcctaa tgccagcatt ttcccggcca gtaacggacc
     7081 gctcatagcc cccagacgcc ctaccgccac ggctgttccc acacctgttg cgcggatctg
     7141 cgaactgtaa aacaacggtg ccagggcata caaaacgctt tgcccacctg tcgcaaacaa
     7201 ccccgcgaca aatcccgcca gcaacatacc gttaaatgac gacaccgttc caagcgccag
     7261 cagcgaagct aacatgccgc tataaatcag tagcgacatg gttactggac gcagcttatc
     7321 catcaatgcg cccaacatta acgtcccgct tgccgccccc atttgcaggg caaacatcac
     7381 ccctgccgcc tgcgatggct ggaatccttg ctccaccaaa agtagcggta gccagttgat
     7441 caacatataa accaccagca gagtgaagaa ataacacaac cacagcagca gcgtcgcggt
     7501 tgccgtttct ggcgcaaata aggcacgcag tggtggcgca gcctgttttt cgccagcgaa
     7561 aaccgctgac tccggcagcc agcgcattaa tagcggcacc agaatcaacg gcaccacacc
     7621 acctacccaa aacaccgttt gccatgctaa gtttgccccc gcgaaaccca gtgtcgccgc
     7681 cagcgccgcg ccaatgggaa caccgcaata catcaggctc actgccgtcc cacgaaaacg
     7741 tggacccgcg gcttcagatg tcagggcgat aagattcggc aacgccgccc ccagcccgac
     7801 accggtcatc agccgcgcaa agaccagtga ggggaaatcc caggcaatcg ccgttgccag
     7861 tgagaacaaa ccaaacagcg caactgagcc aatcaaaata cgcttacgac cataacggtc
     7921 tgccagcatc ccgccaacca acgcgccggg tagcaaaccg agtattccgg cgctaaatat
     7981 ccagcccatt tgcattttat cgagtgcgaa agcctgggcg atgccacccg ccgcaatgcc
     8041 agccgcctga agatccagcc cttccatcag agcgaccaaa aaacaaagcc cgatggtcag
     8101 catcaggcgg gatgaagatg atgaaggggt acgagtcgac ataagatgaa cctcattgcg
     8161 agtgcaggtg aatcgcgact gatacattgc cagccgcgta atcagaacta tttaatgact
     8221 gcctacgggc gaaatgtagc gaaatgtagg ccggataagg cgttcacgcc gcatccggca
     8281 gtcgtgcgca gatgcctgat gcgacgctaa cgcgtcttat cagacctaca aattctcacg
     8341 ccatccgtag gccggacaag gcgttcacgc cgcatccggc tgtcgtgctg ggatgggcat
     8401 acaaaatgca cgttatcggc agtcatcatt atttgttgtt gcgcagatcc agcgccacgt
     8461 cgacgatcat atcctcctgg ccgccaacca tccggcgttt gcccagctca acgagaatat
     8521 ccaccgcact taagccataa cgcgccgccg ccgtttcaca gtgacgcagg aagctcgagt
     8581 aaacaccagc gtatcccagc gccagcgttt cgcgatcgac tcgtaccgga cgatcctgca
     8641 acggacgcac caggtcgtcg gcggcatcca ttaacgcata gagatcggtc ccatgctgcc
     8701 agcccagttt atccgcggcg gcaataaaca cttccagcgg tgcgttacct gcgcccgcgc
     8761 ccattcccgc gaggctggcg tcgattcggt cgcagccctc ttccaccgcc gcgatagagt
     8821 tcgccacgcc aagactcagg ttatggtgag cgtgcatgcc ggtttgcgtt tctggtttca
     8881 acactgcttt cagggcgcgg aaacggtcac ggatatcgct catgttcatc gcaccgccag
     8941 aatccaccac ataaatacag gtcgcaccgt agccttccat cagctttgcc tgcttggcga
     9001 gattctccgg cgtggtcata tggctcatca tcagaaaacc aacggtgtcc attccgagct
     9061 cgcgggcata ctgaatatgc tgggcggaaa catcagcttc ggtacagtgc gttgccacac
     9121 gaaccacccg cgcgccagcc tgccaggcat ttttcagatc gtgaatagtg ccgattcctg
     9181 gcagcaacaa cgtcgcgatt ttggcgtgcc tcaccacatc cgccgccgct tcaatccatt
     9241 caaggtcgct atgtgcgccg aaaccatagt taaagctgga accttgcaaa ccgtcgccgt
     9301 gggccacttc aatcgaatcc acgcgggcat cgtccagtgc tttggcaatc tggcgaacgt
     9361 tttccagcga atactgatga cgaatggcgt gcataccgtc acgcaatgtg acgtccgaga
     9421 tataaagttt tttaccgttc atgccgcttc tcctgccttg cgcgccagtg actgggccat
     9481 tttttccgct gtcgccagcg cactggaagt cataatatcg aggttgcccg cataggcagg
     9541 cagataatgc gctgcgcctt cgacttccag ccagaccgct gttttcagtc cggagaattg
     9601 ccccacgccc ggtaaattga ccggtttatc ctgcgggata acttcaaact gcacgcgctg
     9661 tttcaggcga taacccggta cgtaagcctg caccgcctca gccatttcat tgattgaggc
     9721 ttcgatatca tcttgtgaag cttcgtcgct caatacatac accgtgtcac gcatcatcaa
     9781 cggtggctct gctgggttaa gcacaataat cgccttccct tttgccgcgc cgcccaccac
     9841 ttcaatggct cgggaagtgg tttccgtaaa ttcatcgata ttggcacgcg tgccaggtcc
     9901 ggcagattta ctggcgatag aagcgataat ttcggcgtaa tgaacacgcg ccacgcgtga
     9961 aactgccgcc accattggaa tggtggcctg gccgccgcag gtgaccatgt tgacgttcag
    10021 ttgatcgacg ttcgcctcga ggttaaccac cggcacgcag taagggccga tggcagcagg
    10081 cgtcaggtca attaagcgaa tatccggttt cgcttcgcgt aaagcggcat cgtttttcac
    10141 atgcgcgccc gcgctggtcg catcaaatac aatgtcgata tcagcaaatt caggcatgtt
    10201 catcagcccg atcacccctt catgggtggt ggcgacgccc atacgtctgg cacgcgccag
    10261 accgtcggac tgaggatcaa tgccgaccat caccgccatc tccagatgct gaccgtgacg
    10321 caaaatttta atcatcagat cggtgccaat gttgccagaa ccgataatgg cgactttacg
    10381 cttactcatg acagacttcc ttttggggct gcgcttgaaa atgtcgcagc aactgaacct
    10441 atgccttcaa tatgggcttc aaaacgatcg cccgcattca ccgccaccat cgggcctaat
    10501 gccccggtaa gaatgatatc tccggcgcgc agcggttcac ccagactggc cattttgcgt
    10561 gccagccaga cggcggcatt aagcggatgt cccaggcatt cgctgccgcg cccgctggaa
    10621 acctcttcgt tattacgcgt catcttcatg gcgcagtttt tcaggtctaa ccccgccgga
    10681 cgttgcgccg gaccgccgat gacatacacc ccgcaggaag cgttatctgc cacggtatcg
    10741 acaaactgaa tcgaccagtc gcgaatgcgg ctccccacca cttccagcgc cggaagtacc
    10801 cattcaatgg cgttatacag ttcgtcgaag gtgatatcag ttgcgggtaa atcgcggttc
    10861 aacaccagtg cgatctccgc ttcaatgcgg ggttgcagaa cacgggaaaa aggaatgatt
    10921 tcgttatcgc cataacacat gtcggcaaat aacgtcccaa aatccggttg atcaacgccc
    10981 agttgttgtt gcactttcgg atgcgtcagg cccactttac gccctaccac gcgacgcccc
    11041 tgcacaacgt catattgcac atttatgtgc tgaatggcgt aggccgcttc agcgtgatcg
    11101 ataccaatca gatcgcgcag cggcgcaatc gcttcgccct gctctgcggc gcggcgtaaa
    11161 tccgccgcca gttgctcaag agtatgcttc gtcatgacca ttccttaagg gcgtgcgagg
    11221 aaattcagca ccaattgatt gaaagcgtcg gcatgttccc actgcgccca gtgaccacag
    11281 tcgcggaaga tatgcagttc agaaccggca atgccggaca gcagacgcag acccgcatcc
    11341 atcggcacaa agcggtcgtt gcgcccccag acaatcaggg tttgcgcttt gatttccgcc
    11401 agacgtgggc caaaatccgg gaactgtttc ggattagctt ccaggctctt aacgaagttt
    11461 tccaggtgat cgcggcgcga cagcatatta ttcaggcgcg cttcaaacag ggcgtcggtc
    11521 aaatcgctgg tatcgaaaac gaagatatcc atcatcagct tcaggttttc gatagtcggc
    11581 tgacgataaa gctgattcag tcgcttaata ccttcggttg gcatcggcgt aaacaaactc
    11641 atgccgcccg taccgccgcc catcagcacc agtttgccga cgcgctccgg ccagttaagg
    11701 gtgaacgcca cagaactatg gccgcccatc gagttgccca gcaggtggat tttggcgata
    11761 tccagttgat ccaccacgct tttcaggatt cgtgcattaa gatccgatcg tgaaccacta
    11821 ttaacgatcg aatcgctttt gccccaaccc ggacaatcca gcaggatcac ccgatagccc
    11881 gcctctacca gcggatcgat attgcggctg aagttcgccc agccagtagc acccgggccg
    11941 gaaccatgca gcaggacaac ggtttcgtcg ccttgtccgc agtcattaaa atggatgcgc
    12001 agcgttttac ccgcttcttc tacattcaga aaacggctgg tggcggcttc ggtttgtggc
    12061 tgataactca tcatcttctc ctgcatattc agttctctgt tctggcgctt aacgagccaa
    12121 atccggcaat ccactccggg attgggcggt aataacgccc ttcgctacgc cagttgccaa
    12181 atgcagaaat agcggcaaaa gcggcgaccc aggttttgat ttcatgtgtc gactttccgg
    12241 caatggcgga aagctcttcg ttactgacgg catccagttc ctgtatgcgt ccctgctcca
    12301 gcaaagtcat gaactggtta tcccaaatcg ggttgagcgg atgcagagtt ctctgatcct
    12361 caacaaactt ctcagcggcg ctaatcaccc tttgctgacg caattcgcgc tcactggcgg
    12421 gtaaatcttt cccgctcccc aacagacggt cgcgcatatg ggcatcggct ttcgccagtt
    12481 cgggcaccgg cggctgatgg gaaagcccac cggaacccag gaacagcacg cgtttattga
    12541 gagtgctggt gaaacgtcca atggcttcac ccaacatgcg ggtacgctgg aaaccgggca
    12601 gcggcgtggc gacaccgttg atgaacacag gcagaactgg caccttatcc agcccaccga
    12661 gcaggaactc cagcggctgg gcgaacccgt ggtccacctg catacagtaa gaaacggcaa
    12721 gatcgatccc gctcttcatg acagcatgcg cacaggcctc cgccagctcc acaggcacgg
    12781 gcagctctcc tgccgcactg ccgaaatcac caattgccgt cgctccaacg cctaaacaga
    12841 acggtggcat cacgtcatag aaaaagccgt tgtagtgatc tggcgcaaac agcaccacca
    12901 gttcagggga gaaagccgca atacgctcgc gggcgctggc aatcacgcca ttgacctcat
    12961 cgagtacctc ttgcgccggg tcgacatacc ccaccaacgg cgagtgggaa agacagtgaa
    13021 gataagcgtg catatcaggc taccttttcg acagaaacgt cggcatcagg gcgggtcagc
    13081 gtcatcaccg acgccagttt attcagcgta ttgcccaggg tttgcggaat ggcggtggcg
    13141 gcaacaaagc gatccgggcg catcaccacc agcgaagcat tgtgttgcgc gaaccagcta
    13201 cgcagacgac cttgcgtatc gcccacgtgt agcacgccgt cgtggttatc ctgtgcggta
    13261 tgaatttgca cttccggcac cacctgaatg aagcgtgtgc ccaacgcgcg ccactgctgg
    13321 atttgctcgt cgctcatccc ccacagtgga ttgcatcccc agccaattac cgcgaagttc
    13381 gcgccgatcg cgttatcgag cagcgtcacg tcgccgtttt ccagcgtgac tttcggctga
    13441 ataaacatct tgccgaccgg agagtgcttc gcttcgccct cacgcaccag cgcaccgccg
    13501 taatattgcg gcatcggctt gaagcgcatt tcgaggaagt agcgttttac tggcggcaga
    13561 taattcaaca gccaggaaac gccgtcacgt aacgtaccct gccagcgttt cggcggagcc
    13621 agcacgttgc ccgccgtcac ggacaggtca atcatcgctt tggcgtgatc gcgacgttct
    13681 tgttgatagg tatcgagcag cgcatcgcgg gctttcccct ggataaccaa cgccagtttc
    13741 caggcgaggt taaaggcgtc gcgcatacca ctgttatagc cctgcccctg ccacaccggc
    13801 atgatgtgcg cggcatcgcc cgccagcagt acgcgatcaa tacggaaacg ttgcgccagt
    13861 cgcgcgttat gggtgtagac acgctggcga atcaattcaa cattgtccgg attaggcagc
    13921 actttgctta acagcttgcg catattttgc ggctcacgca gctgctcttc ggtttctccc
    13981 ggcatcacca taaattcaaa gcgacgtacc gcatgaggca gcgcggcaga aacatacggg
    14041 cgcaccggat cgcaacacaa atagatatgc ggcgtactta acggatcgtt ggcgatatct
    14101 accacaatcc actgatttgg cgcagtttta ccttcaaacg gcacattcag ggtgcgacga
    14161 acaaaacttg ccccaccatc acaggccacc agccactggg ctttgatcgt ttcccgctgc
    14221 ccttctgccg ttttcaggtg caaggtcact tcgtcatctt gctggctgaa ggcctccagc
    14281 tcgcgggaaa acaagcagcg cacattcgga aaacgcgaca acccttccag catcaccgca
    14341 tcgacctgtg gctgaataaa ggcgttacgg cgcggccagc caaattcatc ggtcattggc
    14401 tgaatatcag caaaacagcg gccttttggg gtgagaaaac gcatcgcgtg ccacggtgta
    14461 gtgtgcggca gaacattctc gaccaggccg accgactgca tggtgcgcag cgcctcgtca
    14521 tcaataccaa tcgcacgcgg gtagtcgatc aacttatcga gtttctccac caccagcacg
    14581 tcaatgccca tctgaccgag atagttcgcc atcatcagcc caaccggacc ggcaccagcg
    14641 atcgccacct gaacgctatg gttaacagca ggctggatgt cagggtgttg tattgccatt
    14701 tctgcacaac gttgttttaa ctatagaaat gtcaattaat atgcagaaca atgagcagac
    14761 ggaatacaaa accgtgcgcg gcttaacccg cggtctaatg ttattaaata tgttaaataa
    14821 acttgatggc ggtgccagcg tcgggctgct ggcggaactc agcggcctgc atcgcaccac
    14881 tgtgcggcga ctgctggaga cgctgcagga agagggatat gtccgccgta gcccctccga
    14941 tgacagtttt cgtctgacca tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca
    15001 gtggatttct gcactggcgg ccccgctgct gggcgatctg ttgcgcgaag tggtatgacc
    15061 aacagatgtg tccaccctgg atgttgatgc aatggtggta cgcgaaacca cccaccgttt
    15121 cagccgctta tcctttcacc gggcaatggt cgggcgacgt ttaccgctcc tgaaaaccgc
    15181 ctcgggcctg acctggctgg ccttttgccc ggaacaagaa cgcaaggaat taatcgaaat
    15241 gttagccgcc cgccccggtg atgactatca actggcacga gaaccgttaa agctgcaagc
    15301 cattctggcg cgcgcgcgca aagagggtta cggacagaac taccgcggct gggatcagga
    15361 ggagaagatc gcctctatcg ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct
    15421 gaatctggtg tatatggcga gcgcaatgac cattgaacag gcagcggaaa agcatcttcc
    15481 ggcgctacaa cgggtagcaa aacagatcga agaaggggtt gaatcgcagg ctattctggt
    15541 ggccggacgg cgaagcggcg tgcatttacg ttgacaccat cgaatggcgc aaaacctttc
    15601 gtggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
    15661 taacgctata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tctcgcgtgg
    15721 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
    15781 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
    15841 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
    15901 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
    15961 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
    16021 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
    16081 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
    16141 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
    16201 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
    16261 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
    16321 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
    16381 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
    16441 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
    16501 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
    16561 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
    16621 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    16681 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    16741 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    16801 gctcgtatgt tgtgtgaaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    16861 catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
    16921 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
    16981 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc
    17041 ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc
    17101 cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac
    17161 caacgtgacc tatcccatta cggtcaatcc gccttttgtt cccgcggaga atccgacggg
    17221 ttgttactcg ctcacattta atattgatga aagctggcta caggaaggcc agacgcgaat
    17281 tatttttgat ggtgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta
    17341 cggccaggac agccgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaaa
    17401 aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggt agttatctgg aagatcagga
    17461 tatgtggcga atgagcggca ttttccgtga cgtctcgttg ctgcacaaac cgagcacaca
    17521 aatcagcgat ttccatgttg ccactcactt taatgatgat ttcagccgcg cggtactgga
    17581 ggcagaagtt cagatgtacg gcgagctgcg cgatgagctg cgggtgacgg tttctttgtg
    17641 gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga
    17701 gcgtggtggt tatgccgatc gcgtcacgct acgtctgaac gtcgaaaacc cggcgctgtg
    17761 gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg acgacggcac
    17821 gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct
    17881 gctgctgctg aacggcaagc cgttgctgat tcgcggcgtt aaccgtcacg agcatcatcc
    17941 tctgcatggt caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca
    18001 gaacaacttt aacgccgtgc gttgttcgca ttatccgaac catccgctgt ggtacacgct
    18061 gtgcgaccgc ttcggcttgt acgtggtgga tgaagccaat attgaaaccc acggcatggt
    18121 gccaatgaat cgtctgaccg atgatccgcg ctggttaccc gcgatgagcg aacgcgtaac
    18181 gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga
    18241 gtcaggccac ggcgctaatc acgacgcact ctatcgctgg atcaaatctg tcgatccatc
    18301 ccgcccggtg cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc
    18361 gatgtacgcg cgcgtggatg aagaccagcc cttcccggct gtgccgaaat ggtccatcaa
    18421 aaaatggctt tcgctacctg gagagacgcg cccgctgatc ctttgcgaat acgcccacgc
    18481 gatgggtaac agtcttggcg gcttcgctaa atactggcag gcgtttcgtc agtacccccg
    18541 tttacagggc ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa
    18601 tggcaatccg tggtcggctt acggcggtga ttttggcgat acaccgaacg atcgccagtt
    18661 ctgcatgaac ggtctggtct ttgccgaccg cacgccgcat ccagcgctga cagaagcaaa
    18721 acaccagcag cagtttttcc agttccgttt atccgggcaa accatcgaag tgaccagcga
    18781 atacctgttc cgtcatagcg ataacgagct tctgcactgg tcggtggcgt tggatggcaa
    18841 gccgctggca agcggtgaaa tgcctctgga tgttgctcca caagataaac agttgattga
    18901 actgcctgaa ctaccgcagc cggagagcgc cggacaactc tggcttacgg tacacgtagt
    18961 gcaaccgaac gcgaccgcat ggtcagaagc cggacacatc agcgcctggc agcagtggcg
    19021 tctggcggaa aacctcagcg tgacactccc cgccgcgccc cacgccatcc cgcaactgac
    19081 caccagcgaa acggattttt gcatcgagct gggtaataaa cgttggcaat ttaaccgcca
    19141 gtcaggcttt ctttcacaga tgtggattgg cgctgaaaaa caactgctga cgccgctgcg
    19201 cgatcagttc acccgtgcac cgctggataa cgacattggc gtaagtgaag cgacccgcat
    19261 tgacccgaac gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcagc
    19321 gttgttgcag tgcacggcag atacacttgc cgacgcggtg ctgattacga ccgctcacgc
    19381 gtggcagcat cagggaaaaa ccttatttat cagccggaaa acctaccgga ttgatggtag
    19441 tggtcaaatg gcgattaccg ttgatgttga agtagcgagc gatacaccgc atccggctcg
    19501 gattggcctg acctgccagc tggcgcaggt agcagaacgg gtaaactggc tcggattagg
    19561 gccgcaagaa aactatcccg accgccttac tgccgcctgt tttgaccgct gggatctgcc
    19621 attgtcagac atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac
    19681 gcgcgaattg aattatggcc cacaccagtg gcgcggcgac ttccagttca atatcagtcg
    19741 ctacagccaa caacaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg
    19801 cacatggctg aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc
    19861 gtcagtttca gcggaattcc agcttagcgc cggtagctac cattaccagt tggtctggtg
    19921 tcaaaaataa taataaccgg gcaggccatg tctgcccgta tttcgcgtaa ggaaatccat
    19981 tatgtactat ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt
    20041 ttttatcatg ggggcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat
    20101 cagcaaaagt gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca
    20161 accgctgttt ggtctgcttt ctgacaaact cgggctgcgc aaatacctgc tgtggattat
    20221 taccggcatg ttagtgatgt ttgcgc
//
LOCUS       DAZOZY010000061        20161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZOZY010000061 DAZOZY010000000
VERSION     DAZOZY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.061"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(225..1037)
                     /locus_tag="ACHURQ_001919"
     CDS             complement(225..1037)
                     /locus_tag="ACHURQ_001919"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR0823700.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1299..2060
                     /gene="udp"
                     /locus_tag="ACHURQ_001920"
     CDS             1299..2060
                     /gene="udp"
                     /locus_tag="ACHURQ_001920"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR0823701.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2201..3628
                     /gene="rmuC"
                     /locus_tag="ACHURQ_001921"
     CDS             2201..3628
                     /gene="rmuC"
                     /locus_tag="ACHURQ_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR0823702.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3723..4478
                     /gene="ubiE"
                     /locus_tag="ACHURQ_001922"
     CDS             3723..4478
                     /gene="ubiE"
                     /locus_tag="ACHURQ_001922"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR0823703.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4492..5097
                     /gene="ubiJ"
                     /locus_tag="ACHURQ_001923"
     CDS             4492..5097
                     /gene="ubiJ"
                     /locus_tag="ACHURQ_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR0823704.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5094..6734
                     /gene="ubiB"
                     /locus_tag="ACHURQ_001924"
     CDS             5094..6734
                     /gene="ubiB"
                     /locus_tag="ACHURQ_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR0823705.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6813..7082
                     /gene="tatA"
                     /locus_tag="ACHURQ_001925"
     CDS             6813..7082
                     /gene="tatA"
                     /locus_tag="ACHURQ_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR0823706.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7086..7601
                     /gene="tatB"
                     /locus_tag="ACHURQ_001926"
     CDS             7086..7601
                     /gene="tatB"
                     /locus_tag="ACHURQ_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR0823707.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7604..8380
                     /gene="tatC"
                     /locus_tag="ACHURQ_001927"
     CDS             7604..8380
                     /gene="tatC"
                     /locus_tag="ACHURQ_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR0823708.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8422..9204
                     /gene="tatD"
                     /locus_tag="ACHURQ_001928"
     CDS             8422..9204
                     /gene="tatD"
                     /locus_tag="ACHURQ_001928"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR0823709.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9201..9689)
                     /gene="rfaH"
                     /locus_tag="ACHURQ_001929"
     CDS             complement(9201..9689)
                     /gene="rfaH"
                     /locus_tag="ACHURQ_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR0823710.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9856..11349
                     /gene="ubiD"
                     /locus_tag="ACHURQ_001930"
     CDS             9856..11349
                     /gene="ubiD"
                     /locus_tag="ACHURQ_001930"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR0823711.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11396..12097
                     /gene="fre"
                     /locus_tag="ACHURQ_001931"
     CDS             11396..12097
                     /gene="fre"
                     /locus_tag="ACHURQ_001931"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR0823712.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12287..13450)
                     /gene="fadA"
                     /locus_tag="ACHURQ_001932"
     CDS             complement(12287..13450)
                     /gene="fadA"
                     /locus_tag="ACHURQ_001932"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR0823713.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13460..15649)
                     /gene="fadB"
                     /locus_tag="ACHURQ_001933"
     CDS             complement(13460..15649)
                     /gene="fadB"
                     /locus_tag="ACHURQ_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR0823714.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15839..17170
                     /gene="pepQ"
                     /locus_tag="ACHURQ_001934"
     CDS             15839..17170
                     /gene="pepQ"
                     /locus_tag="ACHURQ_001934"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR0823715.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17170..17784
                     /locus_tag="ACHURQ_001935"
     CDS             17170..17784
                     /locus_tag="ACHURQ_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR0823716.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLMTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17823..19274
                     /gene="trkH"
                     /locus_tag="ACHURQ_001936"
     CDS             17823..19274
                     /gene="trkH"
                     /locus_tag="ACHURQ_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR0823717.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19286..19831
                     /gene="hemG"
                     /locus_tag="ACHURQ_001937"
     CDS             19286..19831
                     /gene="hemG"
                     /locus_tag="ACHURQ_001937"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR0823718.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
BASE COUNT         4712 a         5104 c         5488 g         4857 t
ORIGIN      
        1 gttgataata attaatgttt gtttgtgaat gggagttgat ggtaaatctc taaggtgtct
       61 tccattgaag agactcattt atgactatga cttgccgact ttcttaaggt caaatagcgg
      121 agtcgtaatc tccaatcaat tcatactgat tattttttgg gggacagaga tggcagcaaa
      181 acgcactcgt ttactcacca tctcaaatat agactaagag gaaactaact ccgcttctct
      241 ccatactgct taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga
      301 tagctcgggc gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct
      361 gctttcgcat tagcagcccg cagcgcctgg cgcatggttt caacgctctc ctgcggaatg
      421 ctgttatcct gaccgccata taagccgaga atcggcgcgt taagatcggt tgcgatatca
      481 acaggttgtt tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact
      541 gcagctttta gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag
      601 aatccggtga tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcggca
      661 tgatcgagat cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac
      721 gtggggatat cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata
      781 gccagatacc cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca
      841 aaaatttcct gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca
      901 tggtaagcag gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg
      961 tcgtccaggg tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat
     1021 tgttgtgttg ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact
     1081 atagtcagca tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca
     1141 gaatgaagag tgatttatgt gatttgaatc acttttggtg ggtaaattta tgcaacgcat
     1201 ttgcgtcatg gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt
     1261 gcttcttctg actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc
     1321 atctcggcct cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc
     1381 cggatcgtgt ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc
     1441 gcgaattcac tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg
     1501 gtatcggcgg cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca
     1561 ccttcctgcg tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc
     1621 tggttaccac ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat
     1681 tcccggctgt cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatccattg
     1741 gcgcgacaac tcacgttggc gtgacagctt cttctgatac cttctaccca ggtcaggaac
     1801 gttacgatac ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc
     1861 aggcgatggg cataatgaac tatgaaatgg aatctgcaac cctgctgacc atgtgtgcaa
     1921 gtcagggcct gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga
     1981 tcccgaatgc tgagacgatg aaacaaactg aaagccaagc ggtgaaaatc gtggtggaag
     2041 cggcgcgtcg tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt
     2101 gtatttttgc gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa
     2161 ttctattttg tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt
     2221 ttacgcagtt attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca
     2281 tgcgcagcaa aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc
     2341 ggcaaaacaa caaattaccc aaagcgagca ctggcgtgca gagtgcgagt tactcaataa
     2401 cgaagtgcgc agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac
     2461 cacgcggatg gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag
     2521 cgagcagcgc ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa
     2581 tcgccgggtt gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga
     2641 acaactggac ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg
     2701 ccataccctg acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga
     2761 agcgatcaac ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga
     2821 ggtagtattg acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac
     2881 ccaggtcagc atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct
     2941 gccgcaggga aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta
     3001 ttttaacgcc gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt
     3061 gcgtaaccat atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac
     3121 tctggattac gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg
     3181 ccagccggag ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac
     3241 gctgctggtg gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgcaa
     3301 cgcccagcaa atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga
     3361 tgacatgtcc gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat
     3421 gaaaaaactc tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt
     3481 aggagtagaa attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagaa
     3541 tgaagagtat cgacttcggt cggttccgga gcagccgaat gatgaagctt atcaacgcga
     3601 tgatgaatat aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc
     3661 tagggcatcg acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg
     3721 agatggtgga taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac
     3781 aaaaagcgga tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga
     3841 atgatttgat gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg
     3901 gcgtacgccg tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga
     3961 aattctcccg cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca
     4021 tgctcaaaat gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg
     4081 ttcaggcgaa cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt
     4141 cgtttggtct gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc
     4201 tgaaacccgg cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga
     4261 gcaaagccta tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga
     4321 acgacgccga cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata
     4381 ccctgaaagc catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg
     4441 caggggttgt ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt
     4501 aaacctttag tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc
     4561 gcgctgaaaa cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc
     4621 ttttcgacgt cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg
     4681 gcaggcgatg ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat
     4741 cgccagcagc ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag
     4801 gtggtgcaaa acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg
     4861 gccccttata ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca
     4921 aagttcctgc atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa
     4981 gagtggcgta tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc
     5041 gtcgagcgtg ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc
     5101 caggtgaagt acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg
     5161 aactgatccc caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct
     5221 ggatgccaaa tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag
     5281 aactggggcc ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc
     5341 caccgcatat tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca
     5401 agctggcgaa gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg
     5461 acgattttga aatcaagcca ctggcttctg cttctatcgc ccaggttcat accgcgcgat
     5521 tgaaatcgaa tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtta
     5581 ttaaagcgga tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg
     5641 atggtcgccg tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg
     5701 aactgaattt gctgcgggaa tctgccaacg ccattcagct tcggcgaaat tttgaagaca
     5761 gcccgatgct ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga
     5821 tggagcgcat ttacggcatt ccggtgtctg atgttgcgac gctggagaaa aatggcacca
     5881 acatgaaatt gctggcggaa cgtggcgtgc aggtgttctt cactcaggtc tttcgcgaca
     5941 gctttttcca tgccgatatg caccctggca acattttcgt aagctatgaa cacccggaaa
     6001 acccgaaata tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac
     6061 gctatctggc agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc
     6121 tacacgtcga ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca
     6181 ttcgtacggt ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg
     6241 tactgttaaa tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg
     6301 tgttactcca gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc
     6361 tcgatttatg gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta
     6421 ttcctgccct ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag
     6481 aactgcctga attggtttac gacagtttgc gccagggcaa gtatttacag cacagtgttg
     6541 ataagattgc ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc
     6601 tcggaattgg cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgaat
     6661 gggggctgat gcccggctgg ttaatggcag gtggtctgat cgcttggttt gtcggttggc
     6721 gcaaaacacg ctgatttttt catcgctcaa ggcgggtcgt gtaacgtata atgcggcctt
     6781 gtttaatcat catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat
     6841 tgattattgc cgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt
     6901 ccgatcttgg tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc
     6961 aggataaaac cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg
     7021 atacgaatca ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt
     7081 aatccgtgtt tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg
     7141 ttctggggcc gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt
     7201 tgcgttcact ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt
     7261 ttcaggacag tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga
     7321 aagcgtcgat ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa
     7381 acgatcctga aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata
     7441 atgaagctgc gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg
     7501 aacagaagcc agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gctgaaccga
     7561 aaaccgctgc accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac
     7621 tcaaccgctt atcacgcatc taattgagct gcgtaagcgt ctgctgaact gcattatcgc
     7681 ggtgatcgtg atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc
     7741 cgcgccattg atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc
     7801 gccgttcttt acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt
     7861 gattctctat caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct
     7921 ggtggtgccg ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta
     7981 ctttgtggtc tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca
     8041 ggtatccacc gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg
     8101 tgtctccttt gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc
     8161 agaagactta cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt
     8221 gctgacgccg ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt
     8281 tgaaatcggt gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga
     8341 aaacgacgct gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc
     8401 ggttgtcata tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt
     8461 gcgaaagacc gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc
     8521 atcaccggca ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg
     8581 tcctgttggt caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact
     8641 gaagaagcga ttattgagct ggccgcgcag ccagaagtgg tggcgattgg tgaatgtggt
     8701 ctcgacttta accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgcccag
     8761 ctacgcattg ccgcagattt aaacatgccg gtatttatgc actgtcgcga tgcccacgag
     8821 cggtttatga cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc
     8881 tttaccggca cacgcgaaga gatgcaggcg tgcgtggcgc atggaattta tatcggcatt
     8941 accggttggg tttacgatga acgacgcgga ctggagctgc gggaactttt gccgttgatt
     9001 ccggcggaaa aattactgat cgaaactgat gcgccgtatc tgctccctcg cgatctcacg
     9061 ccaaagccat catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt
     9121 gcgcactggc gtggagaaga tgccgcatgg ctggctgcca ccacggatgc taatgtcaaa
     9181 acacggtttg ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta
     9241 atctctttat taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg
     9301 aaaatggcct gaaagccttc gaacgcgcct tcggtaataa tcaccttatc tcccggataa
     9361 ggggttgccg gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc
     9421 gatgggacta tcgctagcga cgcgccaaag cgcacgaagt ggctgacacc gcgggtcgcg
     9481 ttgatagtcg tggtatgaat cacttctgga tcaaattcga caaacaggta gttgggaaac
     9541 aatggctcac tgactgcagt acgttttcca cgcacgattt tttccagggt gatcatcggt
     9601 gccaggcaat tcacagcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc
     9661 ttgcagtaca gtaaatacca agattgcata atgactctta tccgtttaat cggggcgcaa
     9721 ggatagcaaa agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga
     9781 gttcacgcta atttaacaaa tttacagcat cgcaaagatg aacgccgtat aatgggcgta
     9841 gattaagagg ctacaatgga cgccatgaaa tataacgatt tacgcgactt cttgacgctg
     9901 cttgaacagc agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc
     9961 actgaaattg ctgaccgcac tttgcgtgcc ggtgggcctg cgctgttgtt cgaaaaccct
    10021 aaaggctact caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg
    10081 ggcatggggc aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg
    10141 aaagagcccg agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag
    10201 caagtattga acatgccgac aaagcggctg cgtggtgcgc cctgccaaca aaaaatcgtc
    10261 tctggcgatg acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc
    10321 gcgccgctga ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat
    10381 ctgggcattt atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg
    10441 catcgcggcg gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc
    10501 ccggtttctg tggcgctggg tgccgatccc gccacgattc tcggtgcagt cacccccgtt
    10561 ccggatacac tttcagagta tgcgtttgcc ggattgctgc gcggtaccaa gaccgaagtg
    10621 gtgaagtgta tctccaatga ccttgaagtg cccgccagtg cggagattgt gctggaaggg
    10681 tatatcgaac aaggcgaaac tgcgccggaa gggccgtatg gcgaccacac cggttactat
    10741 aacgaagtcg atagtttccc ggtatttacc gtgacgcata ttacccagcg tgaagatgcg
    10801 atttaccact ccacctatac tggtcgtccg ccggatgaac ccgcggtgct gggtgtcgca
    10861 ctgaacgaag tgtttgtgcc gattctgcaa aaacagttcc cggaaattgt cgatttttac
    10921 ctgccaccgg aaggctgttc ttatcgtctg gcggtagtga caatcaaaaa acagtacgcc
    10981 ggacacgcga agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact
    11041 aaatttgtga tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg
    11101 gcgattacca cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt
    11161 gattatctgg attttgcctc gcctgtctcc gggctgggtt caaaaatggg gctggatgcc
    11221 acgaataaat ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcct
    11281 gatgtcgtcg cgcatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa
    11341 agcgcctgat gcgcgttagt tttgccctat ttatttgatc cgacagagaa agcgcatgac
    11401 aaccttaagc tgtaaagtga cctcggtaga agctatcacg gatactgtat accgtgtccg
    11461 aatcgtacca gacgcggcct tttcttttcg tgctggtcag tatttgatgg tagtgatgga
    11521 tgagcgcgac aaacgtccgt tttcaatggc ttcgacgccg gatgaaaaag ggtttatcga
    11581 gctgcatatt ggcgcttctg aaatcaacct ttacgcgaaa gcagtcatgg accgcatcct
    11641 caaagatcat caaatcgtgg tcgatattcc ccacggagaa gcgtggctgc gcgatgatga
    11701 agagcgtccg atgattttga ttgcgggcgg caccgggttc tcctatgcgc gctcgatttt
    11761 attgacggcg ttggcgcgta atcccaatcg cgatatcacc atttactggg gtgggcgtga
    11821 agagcaacat ctgtatgatc tctgcgagct tgaggcgctt tcgctgaagc atcctggtct
    11881 gcaagtggtg ccggtggttg aacaaccgga agcgggctgg cgtgggcgta ctggcaccgt
    11941 gttaacggcg gtcttgcagg atcatggtac gctggcagag catgatatct atattgccgg
    12001 acgttttgag atggcgaaaa ttgctcgtga cctgttttgc agtgagcgta atgcgcggga
    12061 agatcgtctg tttggcgatg cgtttgcatt tatctgagat ataaaaaccc gcccctgaca
    12121 ggcgggaaga acggcaacta aaactttatt cagtggcatt tagatctatg tcgcatccgg
    12181 caatcggtgc acgatgcctg atgcgacgct ggcgcgtctt atcaggccta cattggtgca
    12241 ggatcggtag accggataag gcgttcacgc cgcatccggc aagtggttaa acccgctcaa
    12301 acaccgtcgc aataccctga cccagaccga tacacatcgt cgccagacca aactgaacgt
    12361 ctttgtgttc catcagattc agcaacgtgg tgctgatacg cgcaccggaa caacccagcg
    12421 gatgacccag cgcgatcgca ccaccattga ggttgatctt ctcgtcaatc tgctccatta
    12481 gcccgagatc tttaatgcac ggcaggatct gcgcggcaaa ggcttcgttc atctcaaaca
    12541 cgccgatatc gctggcagaa agccccgctt ttttcagcgc cagcttcgag gcaggaaccg
    12601 ggccgtaacc cataatcgac ggatcacaac caacgaccgc catcgaacgc acacgggcgc
    12661 gcggcttaag acctaattca cgggcgcggc tttcactcat caccagcatg gccgcggcac
    12721 catcagaaag tgcagaagat gtacccgccg ttaccgtacc gcttactgga tcaaacgccg
    12781 gacgcagcgt ggcgagggct tccacggtgg tttccgggcg aatcacttcg tcgtaattaa
    12841 actgcttcag gacgccgtcg gcatcgtgac caccggtcgg gatgatttca tttttaaatg
    12901 cgcccgactg cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag gcatcctgca
    12961 tttcacggct gataccgtgc atacgcgcca gcatttctgc cgttaagccc atcatgcccg
    13021 ccgctttggc gacattgcgg ctcaggccgg ggtgaaaatc gacgccgtga ctcatcggca
    13081 catggcccat atgctccacg ccgccaacca gacatgcctg cgcatcgcca gtcatgatca
    13141 ttcgtgctgc gtcatgcagt gcctgcatgg atgaaccaca caagcgattg acggtaaccg
    13201 ccgggacaga gtgcggcact tctgccagca gcgccgcgtt acgggcgata ttaaaaccct
    13261 gctccagcgt ctgctgcaca caaccccagt aaatatcgtc gagggccgcc gcttccagcg
    13321 ccgggttacg cgccagcagg ctacgcatta aatgagcgga gagatcttct gcacgcacgt
    13381 tacgaaaagc accgcccttc gaacggccca tcggggtgcg aattgcatcg acaatgacaa
    13441 cctgttccat tgtgactcct taagccgttt tcaggtcgcc aaccggacgg gctggctcaa
    13501 ccggaggata gtacggttcg ttatgacgcg ctttattacg cagaccttcc ggcacttcat
    13561 acagcgggcc gaggtgctga tattgctgtg ccatatcgag gtattttgcg ctaccgaggg
    13621 tgtccagcca gcggaacgcg ccgccgtgga acggagggaa gcccaggccg tagaccagcg
    13681 ccatatccgc ttccgccgga gtggcgataa tgccctcttc cagacagcgc accacttcgt
    13741 tgaccatcgg gatcatcatg cgggcgataa tctcttcttc gctgaaatcg cgcttcggct
    13801 ggctcacttc tgccagcagg tcttcaacgg cggcgtcttc ttctttcttc ggcttacctt
    13861 tgctgtcttc tttataacgc cagaaaccga ggccgttctt ctgaccaaag cggttggcat
    13921 caaacagcgc gtcgatggca tcgcggtaat ctttttgcat ccgctgcggg aagcctgctg
    13981 ccatgacagc ctgagcgtga tgcgcggtat caatgcccac aacgtccagc agatatgccg
    14041 ggcccatcgg ccagccaaac tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc
    14101 cgtcgcgcag cagctggctg aaaccggcga aatacgggaa cagcacgcgg ttaacaaaga
    14161 agccggggca gtcgttaacc acaatcggcg tcttgcccat cttgctcgcc caggcgacaa
    14221 ctttcgcgat ggtttcgtcg gagcttttct cgccgcgaat aatttctacc aacggcattc
    14281 ggtggaccgg gttaaagaag tgcatcccgc agaagttttc cgggcgttcc agcgcgttgg
    14341 ccagttcgct gataggaatg gttgaagtgt tagacgccag cacggtatcc tggcgtactt
    14401 tttgttcggt ttctgccagt acggcttttt tcactttcgg gttttcaaca accgcttcta
    14461 ccacaatatc cacgcggtca aatccggcgt agtcgagcgt tgggtggatc gtggagatca
    14521 cgccagccag tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc agcagtttcg
    14581 cggcttcggt catgctgagg gttaacgact tgtcgttgat atctttcatg acaaccggca
    14641 cgcctttcca cgcggactgg taagcgatac cgccacccat gatgcctgcg cccagcacag
    14701 cagcctgttt cggggtttca acgtctttgg tgagtttctt cgctttgcct tttacatatt
    14761 gatcgttaag gaaaatgccg accagtgcgc gagcttcgtt ggtatgcgcc agcgggacaa
    14821 aacttttgtt ttccagattt aaggcttctt cacgaccaaa acgggccgca gcttcaatgg
    14881 tttttactgc ggtgatgggg gccggataat gtttccccgc cgtttgcgcg accattcctt
    14941 tagcgatggt gaagctcatg gtggcttcaa tcttgctcag ttttagcggt tccagcttcg
    15001 gctggcgttt cgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa accgcctttg
    15061 cgccttcaac cagtttttct gctttgacta ccccatccac cagaccgatt ttcagcgcct
    15121 gatccgcgcc gacatcttta ccggcggcaa tgatttccag cgcactgtca gcgcccagca
    15181 tacgtggcat acgtacagaa ccgccaaagc ccggcatgat gcccagtttg gtttccggca
    15241 gaccgatgcg cagatccggc gtcgccagac gataatcggt cgccagcacg cattcgcagc
    15301 caccacccag cgcatagccg ttgacggcag caatggtcgg caccggcaga tcttccaggc
    15361 gattaaacac gctattggca aagtgcagcc actgacttaa ctgttcttca ggaacgagga
    15421 acagggacaa aaattcggtg atatcagcac cgacgataaa ggctgctttg ttcgaacgca
    15481 gcagcagccc ttttagatct gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg
    15541 cgacggtcgc agtgtcgagt ttattaactg aacctggggc atcaaatacc agttcggcaa
    15601 tgccatcttc cagccagtca aggtacaggg tgtcgccttt gtaaagcatg tcagtctcct
    15661 gaatccgcaa ggtgatctgg tcgtaccaga tgcatcgaag tgtgtatttt gtgttaaaaa
    15721 tatgcaaata aaagattaaa gaaatgccga tctgatcacg ctcggcagaa atcacgctct
    15781 ggatgaacga tgtgctaaga tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat
    15841 ggaatcactg gcctcgctct ataaaaatca tatagctacc ttacaagaac ggactcgcga
    15901 tgcgctggcg cgcttcaagc tggatgcatt acttattcac tccggcgagc tgttcaacgt
    15961 ttttctcgac gatcatccct atccgtttaa agtgaacccg caattcaaag cgtgggtgcc
    16021 ggtaactcag gtgccaaact gctggttgct ggtggatggc gtgaacaagc cgaaactgtg
    16081 gttctatctg ccggttgatt actggcacaa cgtcgaaccg ctgccgacct ccttctggac
    16141 tgaagatgtg gaagtgatcg cactgccgaa agccgatggc attggtagcc tgctgcccgc
    16201 tgcgcgcggc aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc aactgggtat
    16261 tgaggccagc aacatcaacc cgaaaggggt gatcgactac ctgcattact accgctcctt
    16321 caaaaccgag tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca
    16381 tcgtgcggca gaagaagcgt tccgttctgg catgagcgag ttcgatatca acatcgccta
    16441 tctgaccgcg accggtcatc gtgataccga cgtaccttac agcaacattg tggcgcttaa
    16501 cgaacacgct tcggtgctgc attacaccaa actggatcat caggcaccgg aagagatgcg
    16561 cagcttcctg ctggatgccg gggccgaata taacggctat gcggctgacc tgactcgtac
    16621 ctggtcggca aaaagcgaca acgactacgc acagctggtg aaagacgtaa atgatgaaca
    16681 actggcgctg atcgccacca tgaaagcagg cgttagctat gtggattacc acatccagtt
    16741 ccatcagcgc atcgccaaac tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga
    16801 agcgatggtc gaaaacgatc tcaccggacc gtttatgccg catggtatcg gtcatccgct
    16861 gggcctgcag gtgcatgacg tagccggttt tatgcaggat gatagcggta cacacctcgc
    16921 ggcaccggca aaatatccgt acctgcgctg cacccgtatt ctccagccgg gtatggtgtt
    16981 aaccatcgaa ccgggtatct acttcattga atcgctactg gcaccgtggc gtgaagggca
    17041 gttcagcaag cacttcaact ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg
    17101 tatcgaagac aacgtggtga tccacgaaaa caacgtggaa aacatgaccc gggatctgaa
    17161 actggcgtga tggaaagctg gttaattcct gcggcaccgg tcacggtcgt tgaagagatc
    17221 aaaaaaagcc gcttcataac gatgttggcg cataccgatg gcgttgaggc ggcgaaagcg
    17281 tttgttgaat cggtgcgggc agaacacccc gatgcccgcc accattgcgt ggcgtgggtg
    17341 gcgggtgcgc cggatgattc tcaacagctg ggtttctctg acgacgggga gccggcagga
    17401 acggcaggta aaccgatgct cgcccagcta atgggcagcg gcgtcgggga aattaccgct
    17461 gtggtagtgc gctactacgg cggcatattg cttggcaccg gtgggttagt gaaagcgtat
    17521 ggcggcggcg tgaatcaggc gctgcgccag ctaatgaccc aacgcaagac gccattaacc
    17581 gaatatactt tgcaatgtga atatcatcag ttaaccggca ttgaagcgtt gctggggcag
    17641 tgtgacggca aaattatcaa cagtgattat caggcattcg ttctgctgcg ggtggcgctt
    17701 ccggcggcga aagtggctga attttccgca aagctggcgg attttagccg tggttcattg
    17761 caattgttag cgattgaaga ataatcccca cttcgttttg cagaactaag gaagcggcag
    17821 agatgcattt tcgcgccatt acccgaatcg ttggactact ggtcatctta ttttcaggga
    17881 ccatgattat ccctgggctg gtagcactca tctaccggga tggagcgggc cgcgctttta
    17941 cccagacctt ttttgtcgcc ctcgccattg gttctatgct gtggtggccg aaccgtaaag
    18001 agaagggcga actgaaatcc cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc
    18061 tgggtagtgt cggtgcgctc ccttttatct tctcggaaag cccgaacctc acgattaccg
    18121 atgcgttttt tgaatctttc tctggcctga ccaccaccgg ggccactacg ctggtggggc
    18181 tggattcgct ccctcatgcc atcctctttt atcgccagat gctgcaatgg tttggcggga
    18241 tggggatcat cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt gggatgcagc
    18301 tctatcgcgc agaaatgccc ggcccgctga aagataacaa aatgcgcccg cgaattgcgg
    18361 aaacggcgaa aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt
    18421 tgtggtttgc cggaatggat gcctttgatg ccatcggcca tagctttgcg actatcgcta
    18481 ttggcggctt ctcgacacat gatgccagta tcggttattt cgacagcccg actattaaca
    18541 ctatcattgc tatcttcctg ctgatctccg gctgtaacta cggtctgcac ttttcactgt
    18601 taagtgggcg tagtctgaag gtttattggc gcgatccgga atttcgcatg tttatcggcg
    18661 tacagtttac gctggtggtt atttgtacac tcgtactgtg gtttcataat gtctacagtt
    18721 cggcgctgat gacaattaac caggcgtttt tccaggtggt atcgatggcg acaaccgccg
    18781 ggtttacgac tgacagcatt gcccgctggc cgctcttttt gccggtactg cttttatgtt
    18841 cagcttttat cggcggttgc gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc
    18901 tgctgctgtt taagcagggg aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct
    18961 atagcattaa gctggggaat cgcgcactgc cggaacgtat cctcgaagcc gtttggggat
    19021 ttttctccgc ctatgcattg gtgtttattg tcagtatgct ggcgattatc gccacgggcg
    19081 tggatgactt ttctgccttt gcttcagttg ttgcgacatt gaataacctg gggccagggc
    19141 ttggcgtggt tgctgataac tttaccagta tgaacccggt ggctaaatgg atcctgattg
    19201 ccaacatgct gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt accccgactt
    19261 tctggcgtga atgatggagt aatacgtgaa aacattaatt cttttctcaa caagggacgg
    19321 acaaacgcgc gagattgcct cctacctggc ttcggaactg aaagaactgg ggatccagac
    19381 ggatgtcgcc aatgtgcacc gcattgaaga accacaatgg gaaaattatg accgtgtggt
    19441 cattggtgct tctattcgct atggtcacta ccactctgcg ttccaggaat ttgtcaaaaa
    19501 acatgcgacg cgactgaatt cgatgccgag cgccttttat tccgtaaacc tggtggcgcg
    19561 caaaccggag aagcgtactc cacagaccaa cagctacgcg cggaagtttc tgatgaactc
    19621 gcaatggcgt cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc cacgttatcg
    19681 ctggtacgat cgttttatga tcaagctaat tatgaagatg tcaggcggtg aaacggatac
    19741 gcgcaaagaa gttgtctata ccgattggga gcaggtggcg aatttcgccc gagaaatcgc
    19801 ccagttaacc aacaaaccga cgctgaaata agcataaaga ataaaaaatg cgcggtcaga
    19861 aaattatttt aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg
    19921 acacggaaca acggcaaaca cgccgccggg tcagcggggt tctcctgaga attccgacag
    19981 agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca caccccgcgc
    20041 cgctgagaaa aagtgaagcg gcactgctct ttaacaattt atcagacaat ctgtgtgggc
    20101 actcgaagat acggattctt aacgtcgcaa gacgaaaaat gaataccaag tctcaagagt
    20161 g
//
LOCUS       DAZOZY010000062        20133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZOZY010000062 DAZOZY010000000
VERSION     DAZOZY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.062"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..875
                     /gene="yieE"
                     /locus_tag="ACHURQ_001938"
     CDS             <1..875
                     /gene="yieE"
                     /locus_tag="ACHURQ_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR0823719.1"
                     /translation="TLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGV
                     QLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILP
                     AVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDEL
                     VVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNS
                     EAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNAL
                     RRFLDYCDPANVPR"
     gene            1080..2549
                     /gene="lysP"
                     /locus_tag="ACHURQ_001939"
     CDS             1080..2549
                     /gene="lysP"
                     /locus_tag="ACHURQ_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR0823720.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2841..4832
                     /gene="cirA"
                     /locus_tag="ACHURQ_001940"
     CDS             2841..4832
                     /gene="cirA"
                     /locus_tag="ACHURQ_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR0823721.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4864..5700)
                     /gene="yeiG"
                     /locus_tag="ACHURQ_001941"
     CDS             complement(4864..5700)
                     /gene="yeiG"
                     /locus_tag="ACHURQ_001941"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR0823722.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5958..6626
                     /gene="folE"
                     /locus_tag="ACHURQ_001942"
     CDS             5958..6626
                     /gene="folE"
                     /locus_tag="ACHURQ_001942"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR0823723.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6643..7800
                     /gene="yeiB"
                     /locus_tag="ACHURQ_001943"
     CDS             6643..7800
                     /gene="yeiB"
                     /locus_tag="ACHURQ_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR0823724.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7984..8982
                     /gene="galS"
                     /locus_tag="ACHURQ_001944"
     CDS             7984..8982
                     /gene="galS"
                     /locus_tag="ACHURQ_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR0823725.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            9262..10260
                     /gene="mglB"
                     /locus_tag="ACHURQ_001945"
     CDS             9262..10260
                     /gene="mglB"
                     /locus_tag="ACHURQ_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR0823726.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10321..11841
                     /gene="mglA"
                     /locus_tag="ACHURQ_001946"
     CDS             10321..11841
                     /gene="mglA"
                     /locus_tag="ACHURQ_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR0823727.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11857..12867
                     /gene="mglC"
                     /locus_tag="ACHURQ_001947"
     CDS             11857..12867
                     /gene="mglC"
                     /locus_tag="ACHURQ_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR0823728.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12994..14229)
                     /gene="preA"
                     /locus_tag="ACHURQ_001948"
     CDS             complement(12994..14229)
                     /gene="preA"
                     /locus_tag="ACHURQ_001948"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR0823729.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14223..15461)
                     /gene="preT"
                     /locus_tag="ACHURQ_001949"
                     /pseudo
     CDS             complement(14223..15461)
                     /gene="preT"
                     /locus_tag="ACHURQ_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(15655..15894)
                     /locus_tag="ACHURQ_001950"
     CDS             complement(15655..15894)
                     /locus_tag="ACHURQ_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR0823730.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15897..16616)
                     /gene="sanA"
                     /locus_tag="ACHURQ_001951"
     CDS             complement(15897..16616)
                     /gene="sanA"
                     /locus_tag="ACHURQ_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR0823731.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16766..17650)
                     /gene="cdd"
                     /locus_tag="ACHURQ_001952"
     CDS             complement(16766..17650)
                     /gene="cdd"
                     /locus_tag="ACHURQ_001952"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR0823732.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17780..18475)
                     /locus_tag="ACHURQ_001953"
     CDS             complement(17780..18475)
                     /locus_tag="ACHURQ_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR0823733.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18472..18870)
                     /locus_tag="ACHURQ_001954"
     CDS             complement(18472..18870)
                     /locus_tag="ACHURQ_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR0823734.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19109..20056
                     /gene="dusC"
                     /locus_tag="ACHURQ_001955"
     CDS             19109..20056
                     /gene="dusC"
                     /locus_tag="ACHURQ_001955"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR0823735.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         4929 a         4974 c         5267 g         4963 t
ORIGIN      
        1 tcaccctccg gcagttggaa gtttttgcag aagtattgaa aagtggatca accacccagg
       61 cgtcggtgat gctggcgttg tcgcaatcag cagtgagcgc agccttgacc gacctggaag
      121 ggcagcttgg cgtgcaactg tttgatcgcg tggggaaaag actggttgtt aatgaacacg
      181 ggcggctgct ctatccgcgt gcgttggcat tgcttgaaca ggcggttgaa atcgaacaac
      241 tgtttcgcga agacaacggc gcgattcgta tctatgccag tagtaccatc ggtaactaca
      301 ttctgcctgc agttatcgcc cgttatcgcc atgattatcc gcagttgccg attgaactta
      361 gcgttgggaa cagccaggac gtgatgcaag cggtgctgga tttccgcgtt gatattggct
      421 ttattgaagg accgtgccac agcactgaaa tcatttctga accgtggctg gaagacgagc
      481 tggtggtttt cgccgcgccg acttcgccgt tggctcgtgg tccggtcacc ttagaacagc
      541 tggccgctgc gccgtggatc ctgcgtgaac gcggttccgg cacgcgggag attgtcgatt
      601 atctgttgct gtcacattta ccgaagtttg agatggcgat ggaattaggt aactccgagg
      661 caatcaaaca tgcggtgcgt catgggttgg gaattagttg cctgtcgcga cgtgtgattg
      721 aagatcaatt gcaggcaggc acattaagtg aagttgcggt ccctctcccg cgcctgatgc
      781 gtacgttgtg gcgtatacat catcggcaaa aacacctttc caacgcgcta cggcgctttc
      841 tggactattg cgatcccgcg aatgtaccgc gttaaggtgc tgtacaagaa catgctggtg
      901 ctgcgtcgat ttcgtgacgc agcgccctca gcatgcattc gccagaaaag agattggctg
      961 ctttacttat aatccctggg cgatcatgaa ggtgtcttat aaccgtgtat ttctgccgga
     1021 aggattgcca atcgtctgct acaatcgcgc ctcattttta agatggatag catttttgta
     1081 tgggttccga aactaaaact acagaagcgc cgggcttacg ccgtgaatta aaggcgcgtc
     1141 acctgacgat gattgccatt ggcggttcca tcggtacagg tctttttgtt gcctctggcg
     1201 caacgatttc tcaggcaggt ccgggcgggg cattgctctc gtatatgctg attggcctga
     1261 tggtttactt cctgatgacc agtctcggtg aattggctgc atatatgccg gtttccggtt
     1321 cgtttgccac ttacggtcag aactatgttg aagaaggctt tggcttcgcg ctgggctgga
     1381 actactggta caactgggcg gtgactattg ccgttgactt ggttgcagca cagctggtca
     1441 tgagctggtg gttcccggat acaccgggct ggatctggag tgcgttgttc ctcggcgtta
     1501 tcttcctgct gaactacatc tcagttcgtg gctttggtga agcggaatac tggttttcac
     1561 tgatcaaagt cacgacagtt attgtcttta tcatcgttgg cgtgctgatg attatcggta
     1621 tcttcaaagg cgcgcagcct gcgggctgga gcaactggac aatcggcgaa gcgccgtttg
     1681 ctggtggttt tgcggcgatg atcggcgtag ctatgattgt cggcttctct ttccagggaa
     1741 ccgagctgat cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac attccacgcg
     1801 cggtacgtca ggtattctgg cgaatcctgt tgttctatgt gttcgcgatc ctgattatca
     1861 gcctgattat tccgtatacc gatccgagcc tgctgcgtaa cgatgttaaa gacatcagcg
     1921 tcagtccgtt caccttggtg ttccagcacg cgggtctgct ctctgcggcg gcggtgatga
     1981 acgcagttat tctgacggcg gtgctgtcag caggtaactc cggtatgtat gcgtctactc
     2041 gtatgctgta caccctggcg tgtgacggta aagcgccgcg cattttcgct aaattgtcgc
     2101 gtggtggcgt gccgcgtaat gcgctgtatg cgacgacggt gattgccggt ttgtgcttcc
     2161 tgacctccat gtttggcaac cagacggtat acctgtggct gctgaacacc tccgggatga
     2221 cgggttttat cgcctggctg gggattgcca ttagccacta tcgcttccgt cgcggttacg
     2281 tattgcaggg acacgacatt aacgatctgc cgtaccgttc aggtttcttc ccactggggc
     2341 cgatcttcgc attcattctg tgtctgatta tcactttggg ccagaactac gaagcgttcc
     2401 tgaaagatac cattgactgg ggcggcgtag cggcaacgta tattggtatc ccgctgttcc
     2461 tgattatttg gttcggctac aagctgatta aaggaactca cttcgtacgc tacagcgaaa
     2521 tgaagttccc gcagaacgat aagaaataag tttcctccct tccttgctaa gccctctcaa
     2581 ccgagagggc tttttcaatt ccatttccct gacaaatcat gcggatataa aatttaacat
     2641 ttggattgat aactgttatc gtttgcatta tcgttacgct acaatcaaaa aaggctgaca
     2701 aatcagaggc tggtccggct ttctgggatg atcacctgca tacaaataag tccaccgcga
     2761 tgctgccgta cgcaagggga cgtaaagaag atgtgagcga tacccatttt attttcgtag
     2821 ttacctcatg gagatatgga atgtttaggt tgaacccttt cgtacgggtc gggctgtgtt
     2881 tgtccgctat ttcttgtgta tggcctgtgt tagcggtcga tgacgatggc gaaacgatgg
     2941 ttgtcactgc atcttccgtt gaacaaaacc ttaaagatgc acccgccagt atcagcgtca
     3001 ttacccagga agatctgcag cgaaaaccgg tacagaatct gaaggatgtc ctcaaagaag
     3061 tgcctggcgt acaactgacg aacgaagggg ataaccgtaa gggcgttagt attcgtggtc
     3121 tggacagcag ctataccctg attctcgtcg acggtaaacg cgtgaactcc cgcaatgccg
     3181 tcttccgcca caatgatttc gatctgaact ggatcccggt cgattccatc gaacgtattg
     3241 aagtggtccg tggcccgatg tcgtcgctgt acggttccga tgcgctcggc ggtgtagtga
     3301 atatcatcac caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc gataccacca
     3361 ttcaggaaca tcgcgatcgc ggtgatacct ataacggtca gttctttacc agtggaccat
     3421 taattgatgg tgtgctggga atgaaagctt acggcagcct ggcaaaacgt gaaaaggatg
     3481 acccgcaaaa ctcaacgacc accgataccg gagaaacgcc gcgtattgaa ggattctcca
     3541 gccgcgacgg caatgtcgaa tttgcctgga caccgaatca aaatcacgat tttactgccg
     3601 gatacggttt cgaccgtcag gatcgtgatt ccgactcgct ggacaaaaac cgcctggaac
     3661 gccagaacta ctccgtcagc cataatgggc gttgggatta cggcaccagc gaactgaaat
     3721 actacggtga gaaagtcgag aacaaaaacc ctggcaacag cagcccgata acttccgaaa
     3781 gcaatacggt cgacggcaaa tacacgttgc cgctgacggc gattaatcag tttctcacgg
     3841 ttggcggtga atggcgtcac gacaaactta gcgatgcggt gaacctgacc gggggaacca
     3901 gctccaaaac gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg cggatcttcg
     3961 agccgctggc gctgacgacc ggcgtgcgta tggacgatca cgaaacctac ggtgaacact
     4021 ggagtccgcg tgcctacctg gtttataacg ccaccgacac cgtaacggtg aaagggggct
     4081 gggcgacggc atttaaagca ccttctctgt tgcaacttag ccctgactgg acgagcaatt
     4141 cctgccgtgg cgcatgtaag attgtgggta gcccggatct gaaaccagaa accagcgaaa
     4201 gttgggagct ggggctttac tacatgggtg aagaaggctg gctggaaggg gttgaatcca
     4261 gcgttaccgt tttccgtaac gatgtgaaag atcgtatcag catcagccgt acgtctgacg
     4321 tcaacgctgc accgggctac caaaactttg ttggttttga gacgggcgct aacggacggc
     4381 gcataccggt atttagctac tacaacgtta acaaagctcg tattcagggc gtggaaaccg
     4441 aactgaaaat tccgttcaac gatgaatgga aactgtcgat caactacacc tacaacgatg
     4501 gtcgtgatgt cagcaacggc gaaaacaaac cgctatccga tctgccgttc catactgcta
     4561 acggtacgct ggactggaaa ccgctggcgc tggaagactg gtcattctat gtttctggtc
     4621 actataccgg gcagaaacgc gccgacagcg cgacggctaa aacaccgggc ggttatacca
     4681 tctggaatac cggcgcggcc tggcaggtga ctaaagacgt caaactgcgc gcaggcgtgc
     4741 tgaaccttgg cgacaaggat ctcagtcgtg acgactacag ctataacgaa gacggacgtc
     4801 gttactttat ggcagtggat tatcgcttct gatgagaaga tgcccggcga accgggcgga
     4861 ctttcacttc agtaaatact gcgcatggaa gcgcaggtga tcctctataa aagaggcgat
     4921 gaagtagtaa ctgtgatcat atcccggctg aatacgcagc gtcatcggcc acgctttctg
     4981 gcgcgcggct tctgccaata ccgcaggttg caactggtcg gcaagaaact gatcattatc
     5041 gccctgatcg ataagcgtcg ggatcgcatc ctgcgcgtta ctggcataca tcagcgcgca
     5101 actatcccat tccagccatg catttttgtc ctcacctaaa taggtgctaa acgctttgat
     5161 tccccacggg acgctgcacg gattcacaat tggcgcaaag gccgaaacgc tggtgtattt
     5221 acccggattt ttcagcgcca taatcagcgc accgtgacca cccattgagt gaccgctaat
     5281 ggcgcagcgg tcgctgacat taaattgcga ctgaaccagc gccggtaatt catcgcgcag
     5341 ataatcatac atccggtaat gcgtcgccca cggcggttgc gtggcattaa gataaaagcc
     5401 tgcgccctgg cctaaatcgt agccatcgtc gttggcaacc ttttcgccgc gcgggctggt
     5461 gtctggcatc accagtacaa tccccagttc tgccgccacg cgctgggcac ccgccttggt
     5521 ggtgaagttc tcgtcattgc aggttaatcc ggaaagccag tacagcactg gtggcggggt
     5581 gtgatcacga gggggtggga gaaagatact gaacgtcatc gggcagttta aggtactgga
     5641 gtcgtgtcgc cagcgttgct gccagccttc aaaacagcgg tgctcttcga gcatttccat
     5701 gcgtggctcc ttgttgtgtt gtttgcaatt tcttcatcat accgtttatt caatgtgctg
     5761 tgagtaactt tcacttccgt atttgcataa cgatgtttta acatctgctg atgaaagtca
     5821 gcggcaatta caataattat cgctgtgaat actggattat gtgcgccgcc tcacgcacaa
     5881 taatcaggct gtaaatcagc ttaataactt tgcccccacg cagggcggag gcgtcacacc
     5941 tgcaggagaa atcataaatg ccatcactca gtaaagaagc ggccctggtt catgaagcgt
     6001 tagttgcgcg aggactggaa acaccgctgc gcccgcccgt gcatgaaatg gataacgaaa
     6061 cgcgcaaaag ccttattgct ggtcatatga ccgaaatcat gcagctgctg aatctcgacc
     6121 tggctgatga cagtttgatg gaaacgccgc atcgcatcgc taaaatgtat gtcgatgaaa
     6181 ttttctccgg tctggattac gccaacttcc cgaaaatcac cctcattgaa aacaaaatga
     6241 aggtcgatga aatggtcacc gtgcgcgata tcactctgac cagtacctgt gaacaccatt
     6301 ttgttaccat cgatggcaaa gcgacggtgg cttatatccc gaaagattcg gtgatcggtc
     6361 tgtcaaaaat taaccgtatc gtgcagttct ttgcccagcg tccgcaggtg caggaacgtc
     6421 tgacgcagca aattcttatt gcgctacaaa cgctgctggg caccaataac gtggctgtct
     6481 ccattgatgc ggtgcattac tgcgtgaaag cgcgtggcat ccgcgatgca accagtgcca
     6541 caacaacgac ctctcttggt ggattgttca aatccagtca gaatacgcgc cacgagtttc
     6601 tgcgcgctgt gcgtcatcac aactgattaa aaggcaggaa ccatggagcg caacgtaacg
     6661 ctcgattttg ttcgcggcgt cgccattctg gggatcctgc tattaaacat cagcgccttt
     6721 gggctaccaa aggcggctta tctcaatccc gcctggtacg gcgctattac gccgcaggat
     6781 gcatggacct gggcatttct cgatctcatc ggccaggtga aattcctcac gctttttgcg
     6841 ctgctgtttg gtgcgggcct gcaaatgttg ctgccccgtg gcagacgctg gatccagtcg
     6901 cggttaacgc tgttagtctt gctgggtttt attcacggtt tattgttctg ggacggcgat
     6961 attctgctgg cttacgggct ggtgggctta atctgctggc ggctggtgcg cgatgcgcca
     7021 tcggtaaaaa gcctgtttaa tacaggcgtc atgctttatc tggtggggct tggcgttttg
     7081 ctgttattgg ggctgatttc cgacagccag accagccgca cctggacgcc ggatgcatcg
     7141 gctattttgt atgaaaaata ctggaagctt cacggcggcg ttgaagcgat cagtaatcgt
     7201 gccgatggtg ttggcaacag tttactggca ctgggcgcac agtatggctg gcaactggca
     7261 gggatgatgc tcattggtgc ggcgctgatg cgcagcggct ggctgaaagg gcagttcagc
     7321 ttacgtcact atcgtcgtac tggttttgtg ctggtggcga ttggggtgac cattaacctt
     7381 cctgccatcg ccctgcaatg gcagctggac tgggcgtatc gctggtgtgc cttcttactt
     7441 caaatgccgc gggaactgag tgcgccgttt caggcgattg gctatgcgtc gctgttttat
     7501 ggcttctggc cgcaattgag ccgctttaag ctggtgcttg cgatcgcctg cgtcggacgg
     7561 atggcgctga ccaactatct attgcaaacg ctgatttgta ccatgctttt ttaccacctc
     7621 ggtttgttta tgcattttga ccgcctggag ctgctggcgt ttgttattcc ggtatggctg
     7681 gcgaatatcc tcttctctgt tatctggctg cgttacttcc gccaggggcc ggtggaatgg
     7741 ctctggcgtc agttaacttt gcgtgctgcc ggaacggcaa tatctaaaac atcaagataa
     7801 cgatctggat cacattcgtt aacaaaacgg ctgtaaccgt ttccattgct gtgactcgat
     7861 tcacgaagtc ctgtattcag tgctgacaaa atagccgcca gcaagcagtc atttactgca
     7921 atctcataac aggtagtgaa tatgatcacc attcgtgatg tagcgcgtta ggctggcgtc
     7981 tctgtggcaa cggtttcccg ggtgctcaat aacagcacgc tggtcagtgc cgacacgcgt
     8041 gaagcagtaa tgaaagccgt gagtgagctg gattatcggc caaacgccaa tgcccaggcg
     8101 ctggcaactc aggttagcga caccattggc gtggtggtga tggacgtttc tgatgcgttt
     8161 ttcggcgcgc tggtaaaagc ggtggatcta gtcgctcagc agcatcagaa atacgtgcta
     8221 atcggcaata gctatcatga agcggaaaaa gagcgtcacg ccattgaggt gttaattcgc
     8281 cagcgttgta atgcgttgat tgttcactca aaagcattga gtgacgatga actggcgcaa
     8341 tttatggata acattcccgg tatggtgtta atcaaccgcg ttgtgccggg gtacgcccat
     8401 cgttgcgttt gcctggataa tctcagcggt gcccgaatgg cgacgcgcat gttgctgaat
     8461 aacggtcatc aacgtattgg ttatctttct tccagtcacg gcattgaaga tgacgccatg
     8521 cgtaaagcag gctggatgag tgcgttgaaa gagcaggata ttattccgcc ggaaagctgg
     8581 attggcactg gtacgccgga catgccgggc ggtgaggcgg cgatggttga actgctgggg
     8641 cgcaatctac aacttaccgc tatatttgct tataacgaca atatggccgc tggcgcactg
     8701 acagcattaa aagataatgg cattgcgatt ccgttacatc tctcaatcat cggtttcgat
     8761 gatattccca tcgcccgtta taccgacccg caattaacga ccgtgcgtta tcccattgct
     8821 tcaatggcta aattagccac cgaactggcc ttgcaggggg cagcaggcaa tattgatcct
     8881 cgtgccagcc actgttttat gccgacgtta gtgcgtcgcc attctgtcgc aacgcgccag
     8941 aatgcggcgg cgatcactaa ctcaacaaat caggcgatgt aaccgctttc aatctgtgag
     9001 tgatttcaca gtatcttaac aatgtgatag ctatgattgc accgttttaa cgttgtaacc
     9061 cgtatgtaac agtgaataat cactttcacc gaggtaatag cgtcataaca acaattaaag
     9121 ccgttttctg gagcgttacc gggcatggaa gaacgaattt aaaaaagtga gcttcggcgt
     9181 tcagtaacac ttcattaact ctactgcccc gccgagcatt tatctcaagc actaccctgc
     9241 ataagaaaaa ccggagatac catgaataag aaggtgttaa ccctgtctgc tgtgatggcc
     9301 agcatgttat tcggtgccgc tgcacacgct gctgatactc gcattggtgt aacaatctat
     9361 aagtacgacg ataactttat gtctgtagtg cgcaaggcta ttgagcaaga tgcgaaagcc
     9421 gcgccagatg ttcagctgct gatgaatgat tctcagaatg accagtccaa gcagaacgat
     9481 cagatcgacg tattgctggc gaaaggggtg aaggcactgg caatcaacct ggttgacccg
     9541 gcagctgcgg gtacggtgat tgagaaagcg cgtgggcaaa acgtgccggt ggttttcttc
     9601 aacaaagaac cgtctcgtaa ggcgctggat agctacgaca aagcctacta cgttggcact
     9661 gactccaaag agtccggcat tattcagggc gatttgattg ctaaacactg ggcggcgaat
     9721 cagggttggg atctgaacaa agacggtcag attcagttcg tactgctgaa aggtgaaccg
     9781 ggccatccgg atgcagaagc acgtaccact tacgtgatta aagaattgaa cgataaaggc
     9841 atcaaaactg aacagttaca gttagatacc gcaatgtggg acaccgctca ggcgaaagat
     9901 aagatggacg cctggctgtc tggcccgaac gccaacaaaa tcgaagtggt tatcgccaac
     9961 aacgatgcga tggcaatggg cgcggtagaa gcactgaaag cacacaacaa gtccagcatt
    10021 ccggtgtttg gcgtcgatgc gctgccagaa gcgctggcgc tggtgaaatc cggtgcactg
    10081 gcgggcacag tactgaacga tgctaacaac caggcgaaag cgacctttga tctggcgaaa
    10141 aacctggccg atggcaaagg tgcggctgat ggcaccaact ggaaaatcga caacaaagtg
    10201 gtccgcgtac cttatgttgg cgtagataaa gacaacctgg ctgaattcag caagaaataa
    10261 gactgattgg gcgcagtcgt ttactgcgcc cttattgtat ggccaacaag gtaaaattat
    10321 atggtcagct caacgactcc gtcttccggg gaatacttgt tggaaatgag cggtatcaac
    10381 aagtcctttc ctggtgttaa ggcacttgat aacgttaatt taaaattccg gccacattct
    10441 atccatgcat taatggggga aaacggtgca ggaaaatcga cattattaaa atgcctgttt
    10501 ggtatttatc aaaaagactc cggcaccatt ttattccagg gtaaagagat cgatttccat
    10561 tccgccaaag aggcactgga aaatggtatt tcgatggtac accaggagtt aaacctggta
    10621 ttacaacgtt cggtgatgga taacatgtgg ctggggcgat atcccaccaa aggcatgttt
    10681 gtcgatcagg acaaaatgta ccgcgaaacc aaagcgattt ttgatgaact ggatattgat
    10741 atcgatccgc gtgcgcgcgt cggcacatta tccgtttcgc aaatgcagat gatcgaaatc
    10801 gccaaagcgt tttcctataa cgcgaaaatt gtgattatgg atgaaccgac ttcttcgtta
    10861 accgaaaaag aggtcaatca tctgttcact attattcgta aattaaaaga gcgcggctgc
    10921 ggtattgttt atatctcgca taaaatggaa gaaatcttcc agttatgtga tgaagttacc
    10981 gtattgcgcg acggtcagtg gatcgccacc gaaccgctgg caggactgac gatggacaag
    11041 atcatcgcca tgatggttgg gcgttctctt aatcagcgtt ttcctgacaa agaaaacaag
    11101 ccgggcgaag tcatcctcga ggtgcgtaac ctaacgtcac tgcgccagcc gtcgattcgc
    11161 gatgtctcgt ttgatctgca taaaggggag atcctcggta ttgccgggct ggtgggggcg
    11221 aaacgtaccg atattgttga gacgttattt ggtattcgcg agaaatcggc tggcaccatt
    11281 acgttgcacg gcaaacagat caataaccat aatgccaacg aagccataaa ccacggattt
    11341 gcactggtaa ctgaggagcg ccgctcaacg ggaatttatg cctatctgga tattggtttt
    11401 aactcgttaa tttccaatat tcgcaactac aaaaataaag ttggtttact ggataactcg
    11461 cggatgaaaa gcgataccca gtgggtgatt gattcgatgc gggtaaaaac gccgggtcat
    11521 cggacgcaaa ttggttcgct ctccggtggt aatcagcaaa aggtgattat tggtcgctgg
    11581 ctactaacgc aaccagaaat attaatgctc gatgaaccga cgcgcggtat tgatgtcggg
    11641 gcgaagtttg aaatttatca gttaattgcc gaactggcga agaaaggcaa ggggattatt
    11701 attatctcct ctgaaatgcc tgagttgtta gggataacag accgtattct ggtcatgagc
    11761 aatggtctcg tttccggaat tgtcgataca aaaacaacaa cgcaaaacga aattctgcgt
    11821 cttgcgtctt tgcaccttta agattagggg cttcccatga gtgcgttaaa taagaaaagt
    11881 tttcttactt acctgaaaga gggcggtatt tacgtcgttc ttttagtttt gctggcgatt
    11941 attattttcc aggacccaac atttttaagt ctgttgaact taagtaatat tctcacccag
    12001 tcatcggtgc gtattattat cgcgctcggt gtggcagggt taattgtcac ccaggggacc
    12061 gatctttctg ctggtcgtca ggtagggctg gcggcagtgg tggctgcgac attattgcag
    12121 tccatggata acgccaacaa agtgttcccg gaaatggcga cgatgccgat tgcgctggtt
    12181 attctgattg tctgtgccat tggtgcggtg atcggtttga tcaacggtct gattatcgct
    12241 tatctcaacg tgacgccgtt cattaccacg ctcggcacga tgatcatcgt ctatggcatc
    12301 aactcgctct attacgactt tgtcggggcg tcgccaattt ctggttttga cagtggcttc
    12361 tctacctttg ctcagggctt tgtcgcgctg gggagtttcc gtctctctta catcaccttc
    12421 tacgcgttga ttgcggtggc gttcgtctgg gtgttgtgga acaaaacccg cttcggtaag
    12481 aacatttttg ccattggcgg taacccggaa gcggcaaaag tatctggtgt caacgtcggc
    12541 ctgaacctgc tgatgatcta cgcgttgtct ggcgtgttct atgcctttgg cgggatgtta
    12601 gaagccggac gtatcggctc tgccaccaac aacctcggct ttatgtatga gctggatgct
    12661 atcgcggcgt gcgtggtagg cggtgtatcg ttcagcggcg gtgtggggac ggtgattggc
    12721 gtggtgaccg gggtgattat ttttaccgtc atcaactatg gcctgacgta tatcggcgta
    12781 aacccatact ggcagtacat catcaaaggg gcgattatta tcttcgccgt agcgctggat
    12841 tcactgaaat acgcgcgtaa gaaatgatgt gaatgtggtc cgcattcggt cataaacgca
    12901 cataacatca tgatctgtgt ggttttgtag gccggataag gcgttcacgc cgcatccggc
    12961 aacaggaacc aaatgccgga tgtgacgcgg gttctacaac gttaccgggt gttctttctc
    13021 gcctttctta aacttcactt ccccgagctc aatacaaccc accgggcaga cgtgaccaca
    13081 aagcagacaa cccacacatt tctcggtatt acaatgcggt gtgcgggttt tctcgctcca
    13141 ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa cagcgtccac agccaacaca
    13201 tttgtcaaga ttgatacggg gatagacaat ataactgcgg tctaaatctt ccgccgggac
    13261 aatattgtta ttcgccagac ctaccatctc ctgcaacgaa tcaaatccct gatcggcgag
    13321 ataatgcgat aacccgctcg ccatatcttc tacgatgcga tagccgtact gcataatgcc
    13381 ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac tccgccgcat cctcccaggt
    13441 ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt tctggatggg tgcgcatttg
    13501 ctgaatgaag cgcagggcga tcggttttac cgctttaccg gaatatccgg aaatacttga
    13561 ttttccgtta acgatcggca taccgatttt ctgattaaga tcgatattgg tgatggattt
    13621 aacggtgtta atcgccgcaa tgccatctgc gccgccgcgc ttcgccgcca gcgccacttc
    13681 gcacatatca ccgatattcg gcgtcatctt cgccagcatt ggcagcgtgg aaccccgttt
    13741 cactgcccga caatattttt ctaccagctc cgggctttgc ccgacatcgc tacccatcgc
    13801 atgagaagtc atttgcggac aggagaagtt acactcgatc atatccgcgc cagcttcttg
    13861 caccaggcgc gccagctcct cccattgctg ctcattttcc cccatgatcg aagcgatcaa
    13921 tactttgtcc gggtaatctt ctttcagccg acgcagggcg gccagattct cctccagcgg
    13981 atgctcggca atctgctcca tatttttgaa gccgataaaa ccggtatctt ctttcaccag
    14041 atgatcaaaa cgcggcgaga cttcgttggc gataaaaaag ccgatcgttt taaacaccac
    14101 accgccccaa cctgtgtcgt aggctttggc acacatctca tagcagttgc ctaccggcga
    14161 agaagagagg cagaacgggt tgggaaactt cacgccgcaa aaagtaatcg aaagatcttt
    14221 cgttaacatg agcaagctcc ctctaaatag tgatgaatcg cctcggcggc ttctttcccg
    14281 gttttcacgg catagaccac ggttttgtct ccctcaacaa tatcgccagc agcaaagact
    14341 tgcgggtcgc gggtctggta attttgtgtt ttgatggtgt tacgctgcgg ctctaactcc
    14401 gcaaaggcat ctagtctggc atgctgaccg acggcgagaa taattttatc tgccgccatc
    14461 gtcagttcgc ccgataaccg tacatgctta aacgtgactt tattgccttc cacggctact
    14521 ggcgtgaatc catcgatgat cgaaacgccc agctcccggg cgctggtaaa ctctttttcg
    14581 cttgccggaa actcatctaa ctcttcacgc gctacgcaag ttaccgcctg acagccgaga
    14641 actttcagcg tgctggctac gtccatcgcg acatcaccgc cgccgataat taatgcgctt
    14701 tgtggaatgc tgatatcgcc ttgcgcctgt cgtgcacgtt gcaagaagtc gacggcaatc
    14761 tcaacgtcac tatgctcaaa cagcggtagc ccggaaccgc ttgataaccc cacggtgacc
    14821 agtaccgcgc ggttttctgc tttcagctgc tcaagggtga gtgtgttacc gacttcgttg
    14881 ttgcacttaa tggtcacgcc cattttttca atacgggcga tctctgcatc cagtactgat
    14941 tgcggtaacc ggaattgcgg aatgccgtta cgcaatcagc caccgggttg tgcttctttc
    15001 tcatagatcg tgacgtcata accctggttt gtcagtgtca cactggcctg caatccggca
    15061 ggacctgcgc caataatcgc gactttgccg agcgttttag taccgggctg ataaatttcc
    15121 atcccggttt gttgttcaaa atcagtaaca aaacgctgta agcggccgat atcaatggga
    15181 gcatcaacac cggcacgggt acaaccgctt tggcataatt tctccgtcgg gcacactctg
    15241 gcacaaacgg caccgagggc attattttcg cgaattgtct cggcagcgcc tttaaaatta
    15301 cgaaagtaga ttgagcgaat aaatttcccc ggatcggtct gtgccgggca agcctgacta
    15361 cagggagcgt catgacataa taaacagcga gaggcttctt taatcgccag taaaggcgta
    15421 aaagccggag tgagttcatc cagataattt tgttgcggca tgtgatcttc cttaatgcta
    15481 tgagtaatta aggcgaacgc taaataaata ttgcgtgctg atattaaggc taagcgtgtg
    15541 gggaaattta aactgtgaaa ggattcacaa atagctgcgg ttcagaatgt tatatccgca
    15601 gcttattgag tgaagtaata attattctca ataattaaca gaaaagagaa aatattaacg
    15661 atagaaaata agataaacaa tcatgccgat agataaaatt ccgaagccga tatatgccac
    15721 catataagcg aaaaagagtc cgggattttt tgctcgccag acatacaccg gttccggtgc
    15781 atttttaacc cgtttatcaa tcgcgcctgc gcgccagcct ttccatgctt cgcggaaaca
    15841 aaatatcggg ataaggaaaa aaacggcaac gacaaaaaac tgctgtacat ccataactac
    15901 tttccttgtt tcttttgtaa ttcaagtaac tgttcgggtg tgacggcggg atacccctgc
    15961 gcatcttccg gcacctggtg catagccgga atagggacca gcggccctaa aaaacgcggt
    16021 tcacgtctaa aaatataaag gtcagccagc gcaccgaaac gggcggcaaa ttcgcgaata
    16081 cgtactgaca gcatatcttt cggtgacggt acggcataac actgagcctg aatccccata
    16141 tgcagcgcaa taaataatgc ccgctcacag tggaaacgtt gggtgataat aatgaaatca
    16201 ttagtatcga acactttgcg tgtacgcacg atggaatcca gcgtacgaaa gcctgcgtaa
    16261 tcgagaacaa tatctgatgg gtcgacacca gcagcgatta aatctttgcg catggtcatc
    16321 ggctcattat aactttgcaa tgcgttatcg ccgctcagta atagataatt taccttaccg
    16381 ctgttatagg cattaatcgc tccttgaatg cggtagcgat aatactgatt aattacgcca
    16441 gtacgataat attttgctgt gccgagcacc acgccgacct ggcggtaggg gagatcctgc
    16501 aattcgtcgt agatataagg cgcggttttc cagctcatcc agcgatcgag gccgagcaca
    16561 gtcaacagca gcaagccgat caggactaac aggctgagga acacgcgctt taacatgcag
    16621 gagactcgat ctaaggaaaa tttccatcag gctactttac ccgctggttt tgcgcaagaa
    16681 acggcaaact gtttgccggg aatttcgaca ccaggagagg gctggcaagc cggacaaggc
    16741 gttcacgccg catccggcac caggcttaag cgagaagcac gcggtcgata ctgtgacagc
    16801 cgagagcttt cagcgttgcg gaggtggcat cccactgaat caacggcgca tcggcttttt
    16861 ctgccagaac cgcgcgctgg atatccgggt aatcataacc cttgagattc aacagaatta
    16921 acgccccctg caacggtggc agagtcgggt tgaatgcggc gttttcagcg tagctgccgc
    16981 tgaaaatacg accgtcttta cattccagcg cgacaccgct tggcgactta ctgtaaggca
    17041 tgtgcgaacg gtttgccgcc gcaatcgctg cctgagaaag cgcatcaccc gtcagcgcat
    17101 agccgtgatc ctgttcgtcc atcagcagcg ttttaatctc cagatctttc ggcccaaagg
    17161 catccggcag atagtcacgc agcgcgtgtg cctcgcggcc cggcagatga atacgcagat
    17221 ccagaccgct gttcagttca ttcataaact gacggcagtg accacaaggc gtatagttaa
    17281 cggtgatggc tgcaagcgct ttttcaccac tcaaccaggc gtggctgatc gcgctttgtt
    17341 cggcatgaac ggtttgctgc attgttgcac caataaattc catattggca ccgaaatacc
    17401 aggttccgct cacaccgcgc gcaattgcgc caacattaaa attcgacaat ggcgtacgcg
    17461 cacaggccgc cgccagtgga agtagtgcga atgccagcgc gtcttcgtcc agccccgttg
    17521 cgctcttcag cgatgagact tgctccccgg tcaacaaagc ggggaagtac ttgtctgcca
    17581 gaataggttc cagtgcagat tgcaagttat ccgcaagttg ggcaaaagcg gtttgaaaac
    17641 gtggatgcat ggcgttgcct cataatcatg taatgggata acagtttacg aacccgttat
    17701 ggctttatat gtgatctgaa tctcattaat tatgcaacgc gtgaaactta catcaaaaat
    17761 gcgcgacgca tcgcaaattt tagcccatta ctgccagaat aatcgggaaa aggaacggtg
    17821 cgatcagcga agtaattatc ccgcataaca ccagcgccag cgaactaaat gcaccttcct
    17881 gataatccag ctcggcgcag cgcgccgtac cgagggcgtg cgaggctgtc cccattgcca
    17941 gaccgcgcgc agctttggta cgaatacgca tcgcattaag caatgtatgg ccaaataccg
    18001 cgccgaggat gccgacgaaa atcacgcaaa ctgcgctgat tgccggaata ccgccaatac
    18061 tgccgccaac cgccattgca ataggcgtgg tgactgattt cggcaggatt gacgcggcga
    18121 tttccggtga agcgcccatc aacaatgcca cggaagtccc cgtcaccatc gcaaccacgc
    18181 tgccgataaa acagatggtg atgatcgatt tccagcgcgc gcggatctgg tgtagctgct
    18241 catataaagg ataggccagc gcgacgaccg ccggttgcag cagatcgttt agcacttcgc
    18301 tacctttaaa gtagctgtcg taagagatgc cggtcagcat taaaaaagga atgatcacca
    18361 ccattgctac cagcaacggg ttaagcaggg gaaacttata ccgtgccgcc agtttgcggg
    18421 cggcaaaaaa gacaatcaac gtcagcggta atgaccacca gatattcgcc atcattcttc
    18481 tgatcctttc tgacctacga ctttacgttc accgtgtacc agttgtgaac tccagctcac
    18541 caccagaaaa accaccagcg tactgactgc acaggaaacc actaccgggc caaactgtgc
    18601 gcggagcaaa tcaaaatatt gcatgacgcc tacgccaatc ggcacaaaca atagcgccat
    18661 atagcgaatc agtacgtagc accccggatt gacccatttt gccggaagaa tttgcaaggc
    18721 cagcaggaca aacaggatca gcatcccgat gatgctgccc ggaatggtta ccggtagcag
    18781 ggaagcaatg aaaatgcctg catacaggca ggcataaatc aggacgaaag cgcgtaaata
    18841 ttgccagata atgttcagtg tcttactcat aatcaacacc ccagatgaga tggattcatc
    18901 atacaattaa ttcgtaaaac gtgctaccga tcacattaag aaatcagcgc ttattttttc
    18961 gctccccggc attacgcaat gcgcaacgaa ctcaggcata catcaagcat agagagggat
    19021 tacagtttgc agcaatccct ctttgcagac ctttaatccg ttgaccatag gcgctaacat
    19081 agcgcctcat ttttttccgg gtgaagatat gcgtgtgtta ctggctccga tggagggtgt
    19141 gcttgactct ctggtgcgag aattgctgac cgaagttaac gactacgatc tgtgcatcac
    19201 cgagtttgtc cgcgtggtgg atcaactgct gccggtaaaa gtctttcatc gcatttgccc
    19261 tgagctacaa aacgccagcc ggacaccatc cggtacgctg gtgcgcgttc agttgttagg
    19321 tcagttccca caatggctgg cagagaacgc cgcccgtgcg gtggaattag gttcctgggg
    19381 cgtggatctc aattgcggct gcccgtcgaa agcggttaac ggcagcggcg gcggggcgac
    19441 gttactcaaa gatcctgaac tcatctacca gggtgcaaaa gcgatgcgtg aagctgtacc
    19501 ggcgcatttg cccgtcagcg tgaaagtgcg tctgggctgg gacagcggtg agaagaaatt
    19561 tgaaatcgcc gatgcggttc aacaggctgg tgctacggag ctggtggtgc atggacggac
    19621 aaaagagcag ggttaccgcg cggagcatat tgactggcag gcgattggcg agattcgcca
    19681 gcggctgaat attccggtga ttgccaacgg cgaaatctgg gactggcaga gcgcgcaaca
    19741 atgcattgcg atcagcggct gcgacgcagt gatgattggt cgcggggcgc tcaatattcc
    19801 caacctgagc cgggtagtaa aatataacga accgcgaatg ccgtggccgg aggtggttgc
    19861 tttgctgcaa aaatataccc gtctggaaaa gcagggcgat accgggttat atcacgttgc
    19921 gcggattaaa cagtggttga gttatttgcg taaagaatac gatgaagcaa cggaattatt
    19981 tcagcatgtt cgggtgttga ataattcccc tgatattgca agggctattc aggcaattga
    20041 tatcgagaaa ctctaaaaaa ccatagtgat gttttttgac aatgtgttag ctctttatca
    20101 ataggccgca cggttgtttg gtggcgtcaa aat
//
LOCUS       DAZOZY010000063        20033 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZOZY010000063 DAZOZY010000000
VERSION     DAZOZY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20033
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.063"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHURQ_001956"
     CDS             complement(<1..330)
                     /locus_tag="ACHURQ_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR0823736.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHURQ_001957"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHURQ_001957"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR0823737.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHURQ_001958"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHURQ_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR0823738.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHURQ_001959"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHURQ_001959"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR0823739.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHURQ_001960"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHURQ_001960"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR0823740.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHURQ_001961"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHURQ_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR0823741.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHURQ_001962"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHURQ_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR0823742.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHURQ_001963"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHURQ_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR0823743.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHURQ_001964"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHURQ_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR0823744.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHURQ_001965"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHURQ_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR0823745.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHURQ_001966"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHURQ_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR0823746.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHURQ_001967"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHURQ_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR0823747.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHURQ_001968"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHURQ_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR0823748.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13115..13543
                     /gene="uspC"
                     /locus_tag="ACHURQ_001969"
     CDS             13115..13543
                     /gene="uspC"
                     /locus_tag="ACHURQ_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR0823749.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13550..14974)
                     /gene="otsA"
                     /locus_tag="ACHURQ_001970"
     CDS             complement(13550..14974)
                     /gene="otsA"
                     /locus_tag="ACHURQ_001970"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR0823750.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14949..15749)
                     /gene="otsB"
                     /locus_tag="ACHURQ_001971"
     CDS             complement(14949..15749)
                     /gene="otsB"
                     /locus_tag="ACHURQ_001971"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR0823751.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(15916..16902)
                     /gene="araH"
                     /locus_tag="ACHURQ_001972"
     CDS             complement(15916..16902)
                     /gene="araH"
                     /locus_tag="ACHURQ_001972"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR0823752.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITILATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(16917..18431)
                     /gene="araG"
                     /locus_tag="ACHURQ_001973"
     CDS             complement(16917..18431)
                     /gene="araG"
                     /locus_tag="ACHURQ_001973"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR0823753.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(18501..19490)
                     /gene="araF"
                     /locus_tag="ACHURQ_001974"
     CDS             complement(18501..19490)
                     /gene="araF"
                     /locus_tag="ACHURQ_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR0823754.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT         4854 a         5392 c         4757 g         5030 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagggcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca ataatgtgtg
    12601 atgcagatca cacaaaacac tcaattactt aacataaatg tataagtcat acttttgttt
    12661 tgggtgtatt tcaatatgtt aaaaagtttt tcgctacgct agcaagcaaa aatgaaacag
    12721 gaataatcga aatgggatgt tgcgcacagt taaaataaat caccgtaaat aatcatctgc
    12781 tataaataat cactttcatg caataccaga taagctattt ttaaacagac acttaccgca
    12841 caacaaacta attaaataca aagaaaggca acaatcaact taatgttaat gaaatgtttt
    12901 acatatatta accacttgtt taatgcggag aatcagtaca ctattttctt ataattacat
    12961 ttgaaattat atgatcaccg ggagtgattg attaattgtc tttactaata atcagactaa
    13021 tatttacctg tttgaccgag ttgggattgc gtcgtttctc cattaggagt aaagctttaa
    13081 tgtcacctga agttcacaga ataaggaaca ggttatgagc tatagcaata ttcttgtcgc
    13141 tgttgcggtt acaccggaaa gtcagcaact gctggcaaaa gcggtatcta tcgccaggcc
    13201 agtaaaggga cacatcagtt taattactct cgcttccgac ccggaaatgt acaatcaatt
    13261 agccgcgccg atgcttgaag atttacgcag cgtgatgcaa gaagaaacgc aaagctttct
    13321 tgataagtta attcaggatg cggggtatcc cgttgacaag acgtttattg cctacggtga
    13381 gttaagcgaa catattctgg aagtatgtca caaacatcat ttcgatttgg tgatttgcgg
    13441 taatcacaat cacagtttct tttcgcgagc atcctgctcg gcgaaaagag tgattgcctc
    13501 aagtgaggtc gatgtgctgt tagttccact tacgggagat taaccgctcc tacgcaagct
    13561 ttggaaaggt agcaacttta tcgcgctgct ggctttccgc gcttcgcgga actatctgct
    13621 ttaggtcgct aatgaagcac tcctgccagt ggttaatatc gtttttcacg ataacgtcca
    13681 gcatttctgc atgacgggaa atacgttccg ccagcgacat agtcaatgca cgatccagcg
    13741 cagctgcaac ttcgtcacga tcgtaggggt taacaattaa cgccgacgtt aactcgtttg
    13801 ccgctcccgc aaattgcgaa agaacaagaa cgcccgggtt ggctgggtcc tgagcagcaa
    13861 catactcttt tgctaccagg ttcatcccgt cacgcagtgg cgtcactaag cccacgtcag
    13921 agtagcggaa tattttcatc agtaatttac ggtcaaaatg ctgattcaaa taataaagcg
    13981 gcgtccagcc taattgcccg tatttaccat taattcgtcc agcttcattt tcgagctgat
    14041 gacgaatatc ctgataggct tgcacatcac cacgcgacgt tggtgcaatc tgggtataac
    14101 gaattttacc atgatgctgc ggatattttt ccagcaacgc ttcataggcg agaaaacgct
    14161 ctggcaaacc tttggaataa tccagccgtt cgacagaaaa gatattttgt acgtttttca
    14221 gttccgcttt aagttgcgcc agttttggcg gcagtggccc ggcagcctgt ttggctattt
    14281 ctttcggttc aatgccgatc gggtagactt ctgttcgaaa tgctttgccc caggctgtat
    14341 ggctttttgc gctacgtgtc gtgacgcggg tcaggttaga aagacaatcc aggaacgcca
    14401 gacgatcgtt ttctgtctgg aaacccagca aatcataatc acaaagctgt tcaagcaagg
    14461 tgtcatatgt cggcagcgcg ttgaagattt ccggtgtcgg gaaaggaata tgcagaaaga
    14521 aaccaatgcg attattcact ccccgtttgc gtaattcatg cgcaaatggc aacaggtgat
    14581 aatcgtggat ccagataatg tcatcgtctt gcaacagcgg cagtaattta tctgccagca
    14641 acgcatttac gcgtagatag ccgtcccagg caggacgctg aaattgcacc agatcgagcc
    14701 gataatgaaa agcgggccag agaacggcat tggagaattg gttgtagtat tcgtcaaggt
    14761 cctgttcgct gaggttaaaa gaggcccacg taatgttacc ttttttcacc ttttttagcg
    14821 gctgatcctc attccctgtt tcaccactcc agccaaacca cagtccgcct gcggctttca
    14881 gtgcccccag tatgccaacg gcaaggccac cggcactggc ggcgtgctcg tctggtggtg
    14941 caatccggtt agatactacg actaaacgac tcatagtcat cactcctgtt attttctctt
    15001 ttttgttgta atgcggtggt tatcatttca agccagctcc agacatccgg cacacccgcc
    15061 agtcgccatg atgcctgagt tgcacctgtg ccaattttta ctgacattcc gcccagtcgg
    15121 ttaacgactg cgaagccaga ttcatcggtt aaatcatcgc ccagaaatac gggcgttcgc
    15181 ccgataaagg gagcttcctg cataaaagct gcaattgcct cacctttact ggtacctctc
    15241 ggtttgatct cgacaacaca ctttccctgc tgtaacgcca tttgtggcca gatctgagta
    15301 atacgttgcg ctaatgtcat taatgcgtct tcatgctgcg gagcctgacg ataatgcagc
    15361 gcaaaagcca tccctttcgc ctccagctcc gcgccgggat actgagcgat gactgtatgc
    15421 agttgcacgc taatatcacg cgcaatcgca tccggcagat gaacgatatg tgttttacca
    15481 ttgatgtcac ggcgctccgc cccatgcacg cccgctaacg ggaagcgata aggttttgcc
    15541 agtgcgtcaa gctccaccat tgagcgccct gatatcaatg ccaatgcacc atcacttgcg
    15601 gttgccagta gctgtagtcc ttgcagaata ttgtcaggca cgacgacctg atcgggatgc
    15661 ggtttgattt ccgccagcgt tccatcaaga tcaaaaaacc aggcatattt cgcggatagt
    15721 tcaggggttt cggttaacgg ttctgtcacc cggctctcct ccttctcttt tcttattgtt
    15781 ttgcgcagaa cgcgctttac agacatgtaa gtatagacag tgtgacgggg gtcgccattt
    15841 gcaatgaaga acagccaacc gttgtggcgg ctggctgtgg tgggaaaaaa cgttaaattg
    15901 ttgtggaaaa aagcatcaga cagtgcgttt cgctttttgc ttgtaacggt cgaagatcac
    15961 cgctgccagc aggattaagc cgcgaaccac gtactgcgcg aaaggagaaa tattaagcag
    16021 gttcatggcg ttttccacgg tgcctaaaat taagataccc gccaccacat atgagatttt
    16081 tccgatgcca cctttcagag aaacgccacc taaaacgcag gcggagataa caataagctc
    16141 ataaccaatc gacgtcattg gctgcccact agtcatacgt gaagccagaa taattccggc
    16201 tatcgcagat accaggccag agagaacaaa gataataatt ttggtgcgaa caaccggtac
    16261 acccgccaga cgcgcagcct cttcgttccc gccaatcgcc agggtgttac gaccaaaggt
    16321 ggttttattc agcaacaaac caaagataat cagacacgcg acggtgagcc agattggcgc
    16381 aggcagaccg aaccagttag cgtaaccaag ggcaaagaag ctttcatctt cgataccgac
    16441 cgctttaccg tctgaaatga tgtacgccag acctcgaaca atctgcatcg ttgccagtat
    16501 tgtgatcaga gcatttattt tcagtttggc gataacaaag ccattgacca ggccacagag
    16561 aatgccaagc agcaaccccg ctgccacgcc aatccacagg ctttcagtca ggttgataac
    16621 caccgccgtg gtgacacccg cacaggcaat tacggaggcg acagaaaggt caaagtcacc
    16681 ggaagcaagg cagaacaaca tgccacacgc caccatcccc gacatggaaa ttgccaggcc
    16741 caaccctttc atattaatga aggtggcaaa atttgggaca aaaatggcac aggcgataaa
    16801 gagcaccgca aacaccacca gcatgccgta ctgatcccaa atacgcccga agctgaatga
    16861 cgacttaggt gcgccagaac ccgatgtaga aacagaagac atcatactct ccttactcag
    16921 gcaactgcct ggctgacttt aggcatcgca aggctcagtg cctgacgctc atctgcctgc
    16981 tcgtgtaaca attcaccggc gatttcacct tcccgcatca ccacaatccg gtcggcaacg
    17041 ccgaggactt caggtaagtc gctggaggca aacagcaccg ccacaccctg cgccgccagc
    17101 gcataaatta cgttatatat ttcgtgctta gcaccaacat caatgctgcg cgtaggttca
    17161 tccagcaaaa tgaccttcat ctcttccgat aaccagcggc ccagaatggc tttttgctga
    17221 tttccgcctg agagattcat gatcagttgc tcagcgcccg gcgttttgat gttgagcgaa
    17281 cgaatgtgct gatcggcatt gttttcttcc caaccgttgt tgattacaca accgccgagc
    17341 acatgtttgc gtctggcact gatgttgata ttgtcgcgaa cggagtgcac gggaataatg
    17401 ccttccgctt tgcgatcttc cgggcagagc atcatgcctg cggcaatggc gtggctcggt
    17461 ttacgaatat cgatcggctg ttggtcgata taaacctgac cggcggtgat ttgcgtcccg
    17521 ccaaacaagc ctttcattaa ttcgctacgc cccgccccta ccagaccaaa cagcccaaca
    17581 atttcaccac tgcgaaccgc cagacttatt ggcgtacgca cgcctggtgc tttcacagca
    17641 tcaagacgca ggcgctcctc gccataacta cgaggttgcc agccgtagat atcgccaatg
    17701 tcgcgcccga ccatcgcctg caccagcgcg tcgtggtcaa cctgctgcat atcggtaaag
    17761 gttttgacat aacgtccatc tttaaagaca gtgatggcat cgctgagggc aaatatttct
    17821 tccatacggt gagaaacgta taagatcacc cgcccctctt ttcgcagttc acgaataacg
    17881 cggaaaagat tgtcgatttc gcgggcagaa agggagctgg ttggctcatc aaaggcgata
    17941 attttagcgt tacgcgccag cgccttggcg atttcaacca tctgccactg accaatggag
    18001 agatatttca gcggcgtgtc cgggtcaata tccataccaa gatgtttaag ttgtaaaccc
    18061 gcctcataat tcagcaatga gcgattcaca atgccgcctt tatgcggcag ctggccgaga
    18121 tagatgtttt ccgcgacggt catttccggc acgagatgca gttcctggta aataatcgcc
    18181 acacccgcgt taagtgctgc ggtcgtgtcg gaaaaggaca tttcctgccc attaatcact
    18241 acagaacccg tggttggcgc atagttgccg ctgaggattt ttaagagagt tgattttcct
    18301 gcgccatttt cacccatcaa cgcatgaacc tgaccggcat agcagtcaaa actaatatcc
    18361 gtcagcgcct taacgccggg aaacgtttta ccgatgccgc gaaatgagag atacggggta
    18421 gactgttgca taacgtctcc gtgaatcact ggtcgttcac accccctctg catcatgcag
    18481 aggggaattt ttccggcaaa ttacttaccg cctaaacctt ttttctccag ttcttcttta
    18541 aagttgtcac gcgtgatcag caccacgtcg gtaacttcgg taaattttgg cggttcaacg
    18601 tcttttgcta cccagttgta aagcatttcg ctggatttat agccatgtac gtccgggctt
    18661 ggcagcaggg aaccgtagaa gccggttgcc tgtgctttag acagttcgct caacgcatcc
    18721 acaccgttaa tgccaatgcc gatgatatcg gcagctttaa agccctgacc ttccgtcgcg
    18781 cgtacgccgc ccagcacggt gctgtcgttc ataccgacga tcagccaatg tttaacttcc
    18841 ggatgttgaa ccagcattga gttggcagcg tcaaatgccc ccgggatgtc gttagatttg
    18901 gtaggtacct gataaatttg tttttccggg aatccggccg ctttcagcgc atccatagaa
    18961 cccgttgtac ggcgacgtgc cgtatccagt tcgttggcgg taatcgccat caccgcgctt
    19021 tctttgacat cccagccacg tttctgcatc tctttataca gttcctgacc ctgacgttcg
    19081 ccaattttag tcgccgccat catcaccagc ggaacggtat ccattggctt acctttggcg
    19141 ttaacaaact ggtcatccac ggcaatgact ttcatatcgt agccacgcgc tttcgcgacg
    19201 atggcagagc cgagtttggg gtccggagta caaataacga aaccttttgc gccactggca
    19261 gccaggctgt cgatcgcgtt caatgttttt tcgccatccg gcacggcaat cttaataacc
    19321 tcaaacccta aatccttccc ggctttatcg gcaaacttcc attcggtctg gaaccacggc
    19381 tcttccggtt gcttcaccag aaaaccgagc ttcaggttct ccgccatagc ggattgtgac
    19441 ataacggctg ccagaccaat ggctgccagg gctttagtaa atttgtgcat ggttctctcc
    19501 agctttagtg tcgttttgtg tagggcaaaa acgaatgact ttcgttaaat taatcggaaa
    19561 acaaagcatt accttttatc taaaagataa gtgaccgtgt tgacatagtt ttagcgagaa
    19621 attaattctc cataggagag caatatcaca tcgcagaatt acagtgagaa cgtgcataaa
    19681 tttagcggga aaagacataa gggaaagcca atttgtcaga caaattgtcg aatgcacagc
    19741 agattaatcc ataagattag cctggaaatc cttgttgtct ttggtaccca tgcgggatgt
    19801 cttcttttta accagtcaat aggccgcatt acctggcgtt gagtttttga aatggtgtaa
    19861 taaccgcaac tcaaagatgt ggaaaatgca cgtcattcat ttcgccatta attatcactg
    19921 tgctcattaa ttaacagaac acgtataatg agagccatct cgcaaaaatg aaaaaacgtt
    19981 ttataaaatc atcacttcat catgaattca aattcattga ttaatatcaa caa
//
LOCUS       DAZOZY010000064        19822 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZOZY010000064 DAZOZY010000000
VERSION     DAZOZY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.064"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            271..1041
                     /locus_tag="ACHURQ_001975"
     CDS             271..1041
                     /locus_tag="ACHURQ_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR0823755.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1032..1505
                     /locus_tag="ACHURQ_001976"
     CDS             1032..1505
                     /locus_tag="ACHURQ_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR0823756.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1613..2152
                     /gene="dnaT"
                     /locus_tag="ACHURQ_001977"
     CDS             1613..2152
                     /gene="dnaT"
                     /locus_tag="ACHURQ_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR0823757.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2155..2892
                     /gene="dnaC"
                     /locus_tag="ACHURQ_001978"
     CDS             2155..2892
                     /gene="dnaC"
                     /locus_tag="ACHURQ_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR0823758.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2941..3435
                     /gene="yjjA"
                     /locus_tag="ACHURQ_001979"
     CDS             2941..3435
                     /gene="yjjA"
                     /locus_tag="ACHURQ_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR0823759.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3689..5980
                     /gene="opgB"
                     /locus_tag="ACHURQ_001980"
     CDS             3689..5980
                     /gene="opgB"
                     /locus_tag="ACHURQ_001980"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR0823760.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6119..7141)
                     /gene="lgoD"
                     /locus_tag="ACHURQ_001981"
     CDS             complement(6119..7141)
                     /gene="lgoD"
                     /locus_tag="ACHURQ_001981"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR0823761.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7280..8194
                     /locus_tag="ACHURQ_001982"
     CDS             7280..8194
                     /locus_tag="ACHURQ_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR0823762.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8409..9770
                     /locus_tag="ACHURQ_001983"
     CDS             8409..9770
                     /locus_tag="ACHURQ_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0823763.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9819..11474)
                     /gene="tsr"
                     /locus_tag="ACHURQ_001984"
                     /pseudo
     CDS             complement(9819..11474)
                     /gene="tsr"
                     /locus_tag="ACHURQ_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11593..12039)
                     /gene="hpaR"
                     /locus_tag="ACHURQ_001985"
     CDS             complement(11593..12039)
                     /gene="hpaR"
                     /locus_tag="ACHURQ_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR0823764.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12311..13600
                     /gene="hpaG"
                     /locus_tag="ACHURQ_001986"
     CDS             12311..13600
                     /gene="hpaG"
                     /locus_tag="ACHURQ_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR0823765.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            13597..15063
                     /gene="hpaE"
                     /locus_tag="ACHURQ_001987"
     CDS             13597..15063
                     /gene="hpaE"
                     /locus_tag="ACHURQ_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0823766.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15065..15916
                     /gene="hpaD"
                     /locus_tag="ACHURQ_001988"
     CDS             15065..15916
                     /gene="hpaD"
                     /locus_tag="ACHURQ_001988"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR0823767.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15926..16306
                     /locus_tag="ACHURQ_001989"
     CDS             15926..16306
                     /locus_tag="ACHURQ_001989"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR0823768.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16417..17220
                     /gene="hpaH"
                     /locus_tag="ACHURQ_001990"
     CDS             16417..17220
                     /gene="hpaH"
                     /locus_tag="ACHURQ_001990"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR0823769.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17231..18019
                     /gene="hpaI"
                     /locus_tag="ACHURQ_001991"
     CDS             17231..18019
                     /gene="hpaI"
                     /locus_tag="ACHURQ_001991"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR0823770.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18194..19570
                     /gene="hpaX"
                     /locus_tag="ACHURQ_001992"
     CDS             18194..19570
                     /gene="hpaX"
                     /locus_tag="ACHURQ_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR0823771.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19580..>19822
                     /locus_tag="ACHURQ_001993"
     CDS             19580..>19822
                     /locus_tag="ACHURQ_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR0823772.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4725 a         5348 c         5118 g         4631 t
ORIGIN      
        1 ttaacttgtt aacattaaaa aacgtgcccg gttatctatt atcctgattc gtaaaggcca
       61 aacaataaat tcattatagt gataatattc atgtgatagt ttgtttgcgg cgagagataa
      121 ttcgcttttt atcaccgagc ttgtcccgat aaccaggatt aatcagcgca attttgatct
      181 taacctaccg caaaacaacc gtgcttcatg atacaaatgg attctcatta tctgaacaat
      241 acgcagcacg tctatgacaa agggcgagtt atgcaaactg agcaacagcg agccgtaaca
      301 cggctttgta tccagtgtgg attatttctt ttgcaacatg gtgcggaaag cgcgttggtt
      361 gatgagcttt cctcacgact gggtcgggcg ttggggatgg acagcgtcga aagttctatc
      421 tcttcgaacg ccatagtgct gacaaccatt aaagatgggc aatgcctgac atcgacacgt
      481 aaaaatcacg atcgcggcat taatatgcat gtggtgactg aagtccagca cattgtgatt
      541 ctggcggagc accatctgct ggattacaaa ggcgtagaga aacgatttag ccaaattcag
      601 ccattacgtt acccaagatg gctggtagcc ttaatggttg gcctttcttg cgcctgtttc
      661 tgcaaactca ataaaggtgg ctgggatggt gccgtcatca ccttctttgc cagtacggcc
      721 gcgatgtata tccgccagct gctggcacaa cgtcatcttc atccgcagat caacttttgc
      781 ctcaccgctt tcgccgccac caccatttcc ggattgcttt tgcaacttcc cactttcagc
      841 aatacaccca ctatcgcgat ggccgccagc gttctgctgc tggtgccggg atttccgttg
      901 attaatgccg tcgccgatat gtttaaaggc cacatcaata ccggactggc acgctgggcg
      961 atcgccagtc tgctgacact ggctacctgc gtcggcgtgg tgatggcact gacgatttgg
     1021 gggctacgcg gatgggtgtg atcgagtttc tgtttgcatt ggcgcaggat atgatcctcg
     1081 ccgccattcc agcggtcggc tttgcgatgg tgttcaacgt tcccgttcgg gcattacgct
     1141 ggtgtgcgct gcttggcgcg attggtcatg gttcacgaat gatcttaatg accagcgggc
     1201 tgaatattga gtggtcaacc tttatggcct ccatgctggt cggcaccatt ggtattcaat
     1261 ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt ggcggccgtt atccctatgt
     1321 tcccgggcat atcggcttat accgcaatga tttcggcggt aaaaatcagc cagttaggtt
     1381 acagcgaacc gttgatgatt accctgttaa ccaactttct tacagcttca tcgattgttg
     1441 gtgcgttatc catcggtctt tccattcctg gattatggtt gtaccgcaag cgccctcgcg
     1501 tataaaattg cctcgcttaa agcacacgga tgagagacag cctcctctcc tccgtgtgtt
     1561 actataaaag ttatctccct tctcgttcat cgttccatat ttgagaaaca gtatgtcttc
     1621 cagagttttg accccggacg tcgttggtat tgacgccctg gtacacgatc accaaaccgt
     1681 tctggcaaaa gctgaaggcg gtgtggttgc cgtatttgct aacaatgccc cggcgtttta
     1741 tgccgtcacg cctgcacgcc tggctgaact gctggcgctg gaagaaaagc tggcgcgtcc
     1801 gggaagcgat gtcgctctgg acgatcaact ctatcaggaa ccgcaagccg ctcccgttgc
     1861 tgtacccatg gggaaattcg ccatgtatcc ggactggcaa cccgatgccg attttatccg
     1921 cctggcggcg ctatggggtg tggcactaag agagccggtg accaccgaag aactggcctc
     1981 attcattgcc tactggcagg cggaaggtaa agtctttcac catgtgcagt ggcaacaaaa
     2041 actggcgcgc agcctgcaaa tcggtcgtgc cagcaacggc ggactgccga aacgagatgt
     2101 gaatacggtc agcgaacctg acagtcaaat tccaccagga ttcagagggt aacgatgaaa
     2161 aacgttggcg acctgatgca acgcctgcaa aaaatgatgc ctgcccatat caaacctgca
     2221 ttcaaaacgg gtgaagaact actggcctgg cagaaagaac aaggagcgat ccgctccgcc
     2281 gctctcgaac gtgaaaatcg ggcgatgaaa atgcagcgca cctttaaccg ctccggtatt
     2341 cggccactgc atcagaactg ctcctttgag aactatcgcg ttgagtgtga agggcagatg
     2401 aatgcgttaa gcaaagcgcg ccagtacgtc gaagagtttg acggcaacat cgccagcttc
     2461 atcttttctg gtaagccagg aaccggcaaa aaccatctgg cggcggcaat ctgcaacgag
     2521 ctgctgctac gcggtaaatc cgtattgatc atcaccgtgg ccgatattat gtcggcgatg
     2581 aaagatacct tcaggaatag cggtaccagt gaagaacaac tgcttaacga tctgagcaac
     2641 gttgatctgc tggtgatcga tgagatcggc gtgcagaccg aatcgaaata cgaaaaagtg
     2701 atcatcaacc agatcgtcga tcgccgctct tcttccaaac gcccaaccgg gatgctgacc
     2761 aacagtaata tggaagagat gaccaagttg ctgggcgaac gcgtgatgga ccgtatgcgc
     2821 ctgggtaaca gtttgtgggt gatcttcaac tgggatagct accgtagccg ggtaacaggt
     2881 aaagagtatt aatccccgtt tctggcggta tactgcgaca caattccacc ttgagtaatg
     2941 atgatgaaaa ctgttaaaca tcttctgtgc tgtgccattg ccgccagcgc attaatttcc
     3001 accggggtgc atgctgcgtc ctggaaagat gcgctctcca gcgccgccag cgaacttggc
     3061 aaccaaaaca gcacgacaca ggaaggcggt tggtcgctcg cgtcattaac taacttgctt
     3121 agcagcggga accaggcctt aagcgcagat aacatgaaca acgccgcagg cattctgcaa
     3181 tactgcgcga agcaaaagct ggcgtcggta accgatgccg aaaacatcaa gaaccaggtg
     3241 ctggaaaagc tgggcctgaa cagtgaagag caaaaagaag acaccaacta tctggatggc
     3301 attcagggtt tgctgaaaac caaagatggt cagcaactca atctggataa catcggaacg
     3361 actccgctgg cagaaaaggt gaaaaccaaa gcctgcgatc tggtgttaaa gcaggggctg
     3421 aacttcattt cctgaaccgc gaaacgccgc gttttagctg ttacccggct gacgcggctc
     3481 tctcctgccc ttttccctgc tcctggacgg ttttccccct gttacacggg aagcgaccgc
     3541 gatcaaaaaa gtctgtttat aaactaattc ctaacaacat cgcatttttt tgacactaga
     3601 attgcagcaa tatggcgacg ccattacatt gtgcttctca aaaagcttaa acgttggcta
     3661 aacggcccgg tttagtgagg atcgcccgtt gtcagaacta ctctctttcg ccctttttct
     3721 cgcctctgtg ctgatttacg catggaaagc gggacgtaac acctggtggt ttgcagccac
     3781 attaacggtg ctggggctat ttgtcgtttt aaatatcacc ctgtttgcca gcgactattt
     3841 tactggcgat ggtattaacg acgcggttct ctatacctta accaacagcc tgaccggtgc
     3901 tggcgtcagc aaatacatcc tgccgggtat cggcattgtg ctggggctga cagcggtgtt
     3961 cggtgcgctg ggctggatcc tgcgccgtcg tcgccatcat ccgcaccatt ttggttacag
     4021 cctgctggcg ctcttactgg cgctgggttc ggtggacgcc agcccggcat ttcgtcagat
     4081 aacggaactg gtgaaatccc agtcacgcga tggcgacccg gactttgcgg cttattataa
     4141 agagccgtcg aaaactatcc ctgacccgaa actcaacctg gtttatatct acggcgaaag
     4201 tctcgagcgg acctattttg ataacgaagc tttcccggat ctcacgcctg aactgggcgc
     4261 gttgaaaaat gaaggcctgg atttcagcca cacgcagcag ctgccaggaa cggattacac
     4321 gattgcgggc atggtggctt ctcagtgcgg cataccgctg tttgccccct ttgaaggcaa
     4381 cgcctccgcc tctgtctcca gcttcttccc gcagaacatc tgtctgggcg atatcctgaa
     4441 aaactcgggt tatcagaact atttcgtgca gggcgcgaat ctgcgttttg ccggtaaaga
     4501 tgtgttcctg aagtcgcatg gcttcgacca cttatacggc tcagaagagc tgaaaagcgt
     4561 ggtggccgac ccgcactatc gcaacgactg gggattctac gacgataccg ttctcgatga
     4621 agcgtggaaa aagtttgaag agctttcccg ctcaggtcag cgattctcac tgtttaccct
     4681 gacagtcgat acccatcacc cggatggttt tatctcgcgt acctgtaacc gcaaaaaata
     4741 tgattttgac ggtaagccga accagtcatt cagcgcggta agttgcagcc aggagaatat
     4801 cgcggcgttt atcaacaaaa tcaaagcgtc accgtggttt aaagataccg tcatcgtcgt
     4861 ctcttctgac catttagcga tgaacaacac ggcgtggaaa tacctcaata agcaggatcg
     4921 caataacctg ttttttgtca ttcgtggcga caagccgcag caagagacgc tggcagtgaa
     4981 gcgtaacacg atggataacg gcgcgacggt gctggacatt ctcggtggcg ataactatct
     5041 cggacttggt cgtagcagtt tatccgggca gtcgatgtcg gaaatcttcc tcaatatcaa
     5101 agagaaaaca ttggcgtgga agccggatat catccgcctg tggaaattcc ctaaagagat
     5161 gaaagagttc accatcgacc agcagaaaaa catgattgcc ttctcgggta gccatttccg
     5221 tttgccgctg ctgttgcggg tttcagacaa acgcgtggaa ccgctgccgg aaagcgaata
     5281 ctcagcaccg ctgcgtttcc agctggccga tttcgctcca cgcgacaatt tcgtctgggt
     5341 tgaccgttgc tataagatgg cacaactctg ggctccggaa ctggcactct ccaccgactg
     5401 gtgtgtctcg caagggcagc ttggcggtca gcaaattgtt cagcatgttg acaaaacaac
     5461 atggcagggc aaaacggcat ttaaagatac ggtcatcgac atggcgcgtt ataaaggcaa
     5521 tgtcgatacg ctgaaaattg ttgataacga tattcgctac aaagccgaca gtttcatctt
     5581 caacgtcgcc ggtgcgccag aagaggtgaa acagtttagt gggatttccc gtccagagtc
     5641 gtggggccgc tggtccaacg cgcagctggg cgatgaagta aaaatcgagt acaagcatcc
     5701 gctgccaaag aaatttgacc tggtgattac cgccaaagca tacggcaata acgccagccg
     5761 tcctattccg gtacgcgtag gcaatgaaga acaaaccctt gtgctgggca atgaagtgac
     5821 caccaccaca ctgcatttcg ataacccaac cgatgccgac acactggtaa ttgtgccgcc
     5881 ggaacctgtc tcaaccaacg aagggaatat cctcggacac tcgccgcgta agctcgggat
     5941 cggcatggtg gaaattaaag tggtggaacg tgaaggataa ttcctgactg atgtgtaggt
     6001 cgcataaggt gcgacctaca caccttctgc ggcctacaaa acatcattga gaaacagaaa
     6061 aaaacagcgc ccaacacttc cccggacgct gcatggcaac tttaataatc tcgccttatc
     6121 agaaagtaat tacaccctta atcaactcac gattgttaat cacatcgcgc tcgtaggttt
     6181 ctgccagcgt ggcgaacgga tagcgatggg ttaacatcat gtcagcagtg atttttcctt
     6241 ccgccatcag tcgaccgact ttagcaaaat cttccggcgt ggcgttgcgg ctacccatca
     6301 tcgtcgtttc ttttttatgg aattccggat cggagaactg caactcacct ttaaacaggc
     6361 cgacaaatac caccgtgccg ccgtgacgaa tcaaattcac ggtgttattc atcgcatgtt
     6421 gattacctgt cgcgtcgatc actttctgcg ccagcgaacc accaaactgc gcccgtagct
     6481 gcgcgtcaaa atcttcggct gacgggtcca gtaaaggtaa ttccagacgc gttgccacat
     6541 gttcacggcg cgccggactg gtatccgcca ccaccacctg tgcgccatcg gctttagcga
     6601 ttgccgccgc gcccagaccg attggccccg ccccgaccac cagcacctgc tcgccgggag
     6661 caatggctgc gcgacgcacc gcatgagcgc taatagcgaa aggttcaatc aatgctgccg
     6721 cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac cggcacgctc aaatactcac
     6781 taaaaccgcc atcctgatgc acgccaatga ctgaaatttt ttcacagcaa ttggtacgcc
     6841 cgcttttaca cgccgggcat tgctgacagg caacataagg gatcacagca acttgctgac
     6901 catttttaag atcagcaata tttttaccca gcccaacaat ctccccacat atttcatggc
     6961 ctaaaacacg tggataacta aaaaatggtt gatttccacc ccaggcatga atatcggtac
     7021 cgcaaatccc gacagactta atttttatta atgcttcatt gtcacccgga ataggtatct
     7081 cgcgttgttt ccagactaat tctttcggct gctggcaaat taaaacattc atcgtagaca
     7141 tgattcggct ctccattttt tgttgcctta gatatcaatg aaaatgaaaa atggcagcgg
     7201 aacggaaaat ctgttttgtg aaaacacacg cataaaaatg ttttaaatcg ggttttaatg
     7261 gactcacaag gaggaaaata tgagtcgttc acaaaattta cgccacaatg tgattaacca
     7321 ggtcattgat gatatggcgc ggggtcatat cccttcccca cttccatcgc aaagtgcgct
     7381 ggcggaaatg tacaacatca gccgcaccac agtgcgtcat attctcagcc acttacgcga
     7441 atgcggcgtc ttgacgcagg ttggaaacga ctatgttatt gcgcgtaaac ctgaccatga
     7501 tgacggtttc gcctgtacta ccgcttcaat gagtgaacaa aacaaagtgt ttgagcaggc
     7561 ttttttcacg atgataaacc agcgccagtt acgcccaggg gaaacgtttt ctgaactgca
     7621 actggctcgg gccgcgggtg tcagcccggt tgtagtaaga gaatatcttt taaaattcgg
     7681 ccgttataat cttattcaca gcgaaaagcg cggccagtgg agcatgaagc aattcgatca
     7741 atcctacgcc gagcagctgt ttgagctacg cgaaatgctc gaaacacatt cacttcagca
     7801 ctttctcaac ctgcctgatc atgatccccg ctggctgcag gcgaaaacca tgctcgaacg
     7861 tcatcggcta ctgcgtgaca acatcggcaa tagctttcgc atgttctcgc aacttgacag
     7921 agattttcac tccttactgc tttcagctgc cgacaatatc ttttttgatc aatcgcttga
     7981 gatcatctca gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg
     8041 caatatcatt gccgtcgatg agcatatgac cattctcagc gctctgattt gccgcagtga
     8101 ccttgatgcc acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg
     8161 atccattaat gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt
     8221 agcgggtgca cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca
     8281 caatcagtcg actaaaaatg tttttgtgag cggtagtaaa gtcctaaaac tttaacctgc
     8341 cgttaatgtc aaccttgcgt aattcacaat tacgtaaaca attaaaatta taaatacagg
     8401 agtccggcgt ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt
     8461 cgtcggcaga tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac
     8521 gcattcaaac caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc
     8581 gcagttcgct gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg
     8641 aaatcggcgc tttgctctcc gtgttttcac tcgcttacgg gattgcgcaa cttccttgcg
     8701 gcccactatt ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt
     8761 cactgttcca ggcaatgtct ggcatggtgc acaactttac gcagttcgtg ttggtgcgta
     8821 tcggtatggg gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact
     8881 ggttcaacat caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg
     8941 gcgttgccgt aagcccaccg attctggcgg cgatgatgct ggtgatgggc tggcgcggga
     9001 tgtttattac cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc
     9061 gcaaccgcga gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg
     9121 tcaatgcccg ccgagatccg ctcagttttg ccgaatggcg cagcctgttc cgtaaccgta
     9181 caatgtgggg aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg
     9241 cctggcttcc tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga
     9301 tggcggctat ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg
     9361 actggctggt caaaggggga atggctccga ttaaaagccg taagatctgc attattgccg
     9421 ggatgttctg ttctgccgcc tttacgctgg tcgtaccgca agcgacaaca tccatgacag
     9481 cggttctgct gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg
     9541 gcttgatcca cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact
     9601 ttgccagctt catctgcgcc tcttttgcgc cgatcattac tggttttatt gttgatacca
     9661 cccactcatt ccgtctggca ctaatcatct gcggttgcgt caccgcagcg ggggcactgg
     9721 cgtacatctt cctggttcgt cagccgatca acgacccacg taaagattaa tctccttatg
     9781 cccgataaca ttttgcttat cgggcatttt catggcgatt aaaatgtttc ccagttctcc
     9841 tcgctatctg ccacggccat tttacgcggc gcagctggcg tcacggtttt taccacagcc
     9901 gatgtttcac gctgctgttg ctgaatccgg aacactgcca ctgcttcggt caggcgactg
     9961 gcctgctctt ccagcgcggc ggcggcagcg gcagactctt ccaccagcgc ggcgttctgt
    10021 tgagttaccc ggtccatctc agcaaccgct aagccaacct gatcgatacc acggctctgc
    10081 tcatcagaag cagaagcaat ttcgcccata atgtccgtca cgcgggtcac ggcgctgaca
    10141 atctccgcca ttgtttcccc ggcgctttcg accagcgtag agccaacatc cactttcccc
    10201 accgagtctt caatcaggct tttaatttca cgagccgcct gggcgctgcg ctgggccaga
    10261 ttacgcactt ctcccgcgac caccgcaaaa ccgcgccctt gctcacccgc acgcgcagcc
    10321 tcaaccgccg cgttcaaagc cagaatattg gtctggaagg caatgccgtc aattacgctg
    10381 ataatatcgg cgattttctg cgaactggtg gagatatcgc gcatagtctg caccacgtta
    10441 tctaccactt tgccgccgcg ttgcgccgtt tcagaagcac ttaacgccag atggctggcc
    10501 tggcgcgcat tttcggcgtt ctgcttcacc gttgcggtca gttactccat gctggctgcc
    10561 gtctcttcca gcgaagcggc ctgttgctcg gtgcgcgaag agagatcgtt attgccggtg
    10621 gcgatttcgc tggcaccgct atagatggca ttggccccgt tgcgcacatc accgacggta
    10681 cgcatcagct ctccctgcat atggcgcaaa ctctctgcca gttgccccat ctcattagag
    10741 ccatccacct caatcggttt caccagatcg ccgcctgcaa tatgacgaat gctgtcaatc
    10801 aggcgattca ttggcgctac cagcgaggct ttaataccga accagacggc gaagatgacc
    10861 gccagtacga cgatcatcac gcccaccaga atccacatcg cctggctgta ggaggcattg
    10921 ttatcgctga cggcgatatc atagagccga tcgttttgct ccatgtaagc cacatactgc
    10981 ttctcgaaac cgtcctgata tccctgggtc ggctgatcaa agaactcgtt gattttgcct
    11041 gcacctaaca gttggatcag ctccgccagc gcattgtgat aaatatcgta attacgtttg
    11101 atctctgccg ctgcggcggt gctctgacgc gggtcacgcg gcaacgcttc gtaatccgcc
    11161 cagttttttt ccgcctgttt cagcgaaata ctggcactct gcatcagctc agcaacggtt
    11221 gaaccgctac caatattgtt ctgatccatc atgtagcgga tacccgcgcg gttgagggtg
    11281 ttacgcgtct gcaacaacgc gacccagctg ccattcagcg tggattgctg ctggcgaatg
    11341 gtttgtaaaa cagtgaaatt ttctttgtca ttctttaagg cattaaagaa cagaccgcct
    11401 gatgtcagtt gtaaaaggcc aaaaacggcc aaaaccagca gtaagctggt cacgatcttg
    11461 atacgtttta acatggtttc tctttcctgt ggaccgatgc aagattttcg gcctggaaag
    11521 caaaaacttt atgaaaaggt gtaacccacc atccatggaa aaaccaggcg ctggttatca
    11581 gatactaaaa agttattcat tatcgcccgg gatggcttct tgtcgggaat tgcccagagc
    11641 aataaattcc tctaacagat gggttaactg ttgcattttc tcggcagtaa actgagcttc
    11701 aatctgccga taggcttctt caatctgtgt ctgagcgcgg ttatacagcg cctgcccctc
    11761 tttggttagc gagatataga gcttccgctg atcattgatc ggctttaatc gcaacactaa
    11821 accgtcgcgt tccatccgcg tgaggatccc ggtcaggctt gggcgcaaaa tgcaggcgcg
    11881 atacgccaga tcgtgaaaat ccattgatgg gctttccgcc agaatacgca cgatgcgcca
    11941 ctgttgctca gtcaaattat gccgtttaac gattgggcga aaataactca tcgccgcttc
    12001 tcgcgcctgg agcaacgcaa tggttagtga gtcgtgcatt atctttcccc tgaaaaattc
    12061 aaaatcatta atatagaaac agttaatcgt cacgttaccg ggactgcgct gcccctgcaa
    12121 ttcctgattt tatcaaaatt gatgatattt tattaactta ctgatattta atattttatt
    12181 atctttaaat gtaaatagtt tgttaattag atcacattta catcacttac ttttgccaaa
    12241 ctacgcaatc gggaataaaa gcaatcatta acatattaat gattaagatg atcgaatccg
    12301 aggagtggta atgaaaggca ctatcttcgc cgtagcgttg aaccatcgca gccaacttga
    12361 tgcatggcag gaagcgttcc agcaatcccc ctacaaagcc ccgcctaaaa ctgcggtctg
    12421 gtttattaaa ccgcgcaata cggtgattgg ttgcggtgaa ccgattccct ttccacaggg
    12481 tgaaaaggta ctgagcggtg cgactgttgc gctgattgtg ggaaaaacgg cgacgaaagt
    12541 acgtgaagaa gatgcggcag agtacatcgc cggatatgcg ctggctaacg acgtcagcct
    12601 gccggaagag agcttttacc gcccggcaat caaagcaaaa tgtcgtgatg gattctgccc
    12661 cattggcgaa accgtggctc tcagcaatgt cgataatctg accatctata ccgagatcaa
    12721 cgggcgtcct gccgatcact ggaataccgc cgatttacaa cgtaacgccg cgcagttgct
    12781 gagcgccctg agcgaatttg ccacgctgaa tccaggcgac gccattctgc tcggcacgcc
    12841 acaggcgcgc gtggaaatac agccaggcga tcgcgttcgt gttctcgcag aaggtttccc
    12901 gccgctggaa aatccggtag tggacgaacg tgaagtgacc acgcacaaga gcttcccaac
    12961 gcagccacac ccgcacggca cgctgtttgc cctcggcctg aactacgccg accacgccag
    13021 cgaactggaa tttaagccac cggaagaacc gctggtgttc ctgaaagcgc caaataccct
    13081 cactggcgat aaccagacct ccgtgcgtcc aaacaatatt gaatacatgc actatgaagc
    13141 ggagctggtg gtagttattg gcaaacaggc gcgtaacgtc agcgaagccg atgccatgga
    13201 ttatgtcgcg ggctacaccg tgtgtaacga ctacgccatt cgtgactatc tggaaaacta
    13261 ctaccgccct aacctgcggg taaaaagccg cgacggactg acgccgatgc tttcaaccat
    13321 cgtgccgaaa gaggcgatcc cggacccgca taatctgacc cttcgcacct tcgtcaacgg
    13381 cgagttacgc cagcaaggca ccaccgccga tctgatcttc agcgtgccct tcctgatcgc
    13441 ctacttaagt gaatttatga ccctgaatcc gggcgacatg atcgccaccg gcacacccaa
    13501 aggcttatct gacgtggtgc ctggcgatga agtagtggtg gaagtagaag gcgtgggccg
    13561 cctggtgaac cgaattgtga gtgaggatac agcgaaatga aaaaagtaaa tcattggatc
    13621 aacggcaaaa atgttgcagg taacgactac ttccagacca ccaatccggc aacgggtgaa
    13681 gtgctggcgg atgtggcctc tggcggtgaa gcggagatca atcaggcggt agcggcagcg
    13741 aaagaggcgt tcccgaaatg ggccaatctg ccgatgaaag agcgtgcgcg cctgatgcgc
    13801 cgtctgggcg atctgatcga ccagaacgtg ccagagatcg ccgcgatgga aaccgcggac
    13861 accggcctgc cgatccatca gaccaaaaat gtgttgatcc cacgcgcttc ccacaacttt
    13921 gaatttttcg cggaagtctg ccagcagatg aacggcaaga cctatccggt tgacgacaag
    13981 atgctcaact acacgctggt gcagccggtg ggcgtttgtg cgctggtatc gccgtggaac
    14041 gtaccgttta tgaccgccac atggaaggtc gcgccgtgtc tggcgctggg caataccgcg
    14101 gtactgaaaa tgtcggaact ctccccgctg accgctgaca gcctgggtga gctggcgctg
    14161 gaagccgata ttccggcagg cgtgctgaac gtggtacagg gctacggcgc aaccgcaggg
    14221 gatgcgctgg ttcgtcatca tgacgtacgt gccgtgtcgt tcaccggcgg tacggccacc
    14281 gggcgcaaca tcatgaaaaa cgccgggctg aaaaaatact ccatggaact gggcggtaaa
    14341 tcgccggtgc tgatttttga agatgccgat attgaacgcg cgctggacgc cgccctgttc
    14401 accatcttct cgatcaacgg cgaacgctgc accgccggtt cgcgcatctt tattcagcaa
    14461 agcatctacc cggaattcgt taaacgcttt gccgaacgcg ccaaccgtct gcgcgtgggc
    14521 gatccgaacg atccgaatac ccaggttggc gcgcttatta gccagcagca ctgggaaaaa
    14581 gtctccggct atatccgtct cggcattgaa gaaggcgcaa ccctgctggc gggcggcccg
    14641 gataaaccgt ccgacctgcc tgcgcacctg aaaggcggca acttcctgcg cccaaccgtg
    14701 ctggcagacg ttgataaccg tatgcgagtc gcccaggaag agattttcgg gccggtcgcc
    14761 tgcctgctgc cgtttaaaga cgaagccgaa ggcttacgtc tggcaaacga cgtggagtac
    14821 ggcctcgcgt cgtacatctg gacacaggat gtcagcaaag tgttacgcct ggcgcgtggc
    14881 attgaagctg gcatggtgtt cgtcaacacc cagaacgtgc gtgacctgcg ccagccattt
    14941 ggcggcgtaa aagcctccgg caccgggcgt gaaggcggtg agtacagctt cgaagtgttc
    15001 gcggaaatga agaacgtctg catttccatg ggcgaccatc caattccgaa atggggagtc
    15061 tgatatgggt aagttagcgt tagccgccaa gatcacgcac gtaccgtcga tgtatcactc
    15121 tgaactgcca gggaaaaacc acggttgccg ccagggcgcg atcgacgggc ataaagagat
    15181 cagcaagcgt tgccgggaaa tgggcgtcga taccattatc gttttcgata cccactggct
    15241 ggtcaacagt gcttatcaca tcaactgtgc agaccatttt gaaggcgtct acaccagcaa
    15301 cgagctgccg cattttatcc gcgatatgac ctacaactac gagggcaacc cggagttggg
    15361 gcagcttatt gccgatgaag ccttaaagct cggcgtgcgg gcaaaagcgc acaacattcc
    15421 cagcctgaaa ctggaatacg gcacgctggt gccgatgcgc tacatgaatg aagacaagca
    15481 cttcaaagtg gtctccattt cggctttctg cacggttcac gattttgccg acagccgcaa
    15541 gctgggcgaa gcgattctga aagcgatcga acagtacgac ggcaccgtgg cggtccttgc
    15601 cagcggttcg ttatcgcacc gctttattga cgatcagcgt gcggaagaag ggatgaacag
    15661 ctacacccgc gagttcgacc gccagatgga cgagcgcgtg gtgaagttgt ggcgcgaagg
    15721 ccagttcaaa gagttctgca atatgctgcc ggagtacgcc gactactgct acggcgaagg
    15781 caatatgcac gacacggtga tgctgctggg gatgctcggc tgggataaat acgacggcaa
    15841 ggtggagttt atcaccgagc tattcccaag ctctggcacc ggtcaggtta acgctgtttt
    15901 cccgcttccc gcgtaaggac gttttatgcc gcactttatc gttaaatgca gtgacaacat
    15961 ccgcgaagaa gccgacctgc cggggttgtt cgccaaagtg aatccgacgc tggcagccac
    16021 gggtattttt ccgctggcgg gtattcgcag ccgcgtgcat tgggtcgata cctggcagat
    16081 ggccgacggg cagcgtgatt atgccttcgt gcatatgacg ttgaaaatcg gcgcaggtcg
    16141 cagcctggaa agccgccagc aggcgggtga aatgctgttt gaactgatta aaacgcactt
    16201 cgccgccctg atggagagcc gcctgctggc gttgtcgttt gagattgaag agctgcatcc
    16261 gacgctgaat tttaaacaaa acaacgtgca cgcattgttt aagtagcgcg cagattgccc
    16321 ggtggcgctg cgcttaccgg gcctacaaaa ccccaaaccg tacaccgtag gccggataag
    16381 gcgcagccgc atccggcaat gccacaggat atcgctatgt tcgataaaca cacccacacc
    16441 ctgatcgccc agcgtctgga tcaggcagaa aaacagcgcg aacagatccg cgcgatctcg
    16501 ctggattacc cggagatcac catcgaagac gcttacgcgg tgcagcgtga atgggttcgc
    16561 ctgaaaatcg ccgaaggtcg cacgctgaaa ggccacaaaa tcggcctgac ctcgaaagcg
    16621 atgcaggcaa gctcgcagat cagcgaaccg gattacggcg cgctgctgga tgacatgttc
    16681 ttccacgatg gcagcgatat cccgaccgat cgctttatcg tgccgcgcat tgaagtggag
    16741 ctggctttcg tgctggcaaa accgctgcgt gggccaaact gcacgctgtt cgacgtctac
    16801 aacgccacgg actacgtgat cccggcgctg gagctgatcg acgctcgctg ccacaacatc
    16861 gatccggaaa cccagcgccc gcgcaaagtg ttcgacacca tttctgataa cgccgccaac
    16921 gccggggtga tcctcggcgg tcgtccgatc aaaccagacg aactggatct gcgttggatc
    16981 tccgccctga tgtatcgcaa tggcgtgatt gaagaaaccg gcgtcgccgc tggcgtgctg
    17041 aatcatccgg caaacggcgt ggcctggctg gcgaacaaac tcgcaccgta tgacgtacaa
    17101 ctggaagccg ggcaaatcat tctcggtggt tcgttcaccc gcccggttcc ggcgcgtaag
    17161 ggcgacacct tccacgtcga ttacggcaac atgggttcca ttagctgccg ctttgtttaa
    17221 ggagagaacg atggaaaaca gctttaaagc ggcgctgaaa gcaggccgtc cgcagatcgg
    17281 attatggctg gggctgagta gcagctacag cgcagagtta ctggccggag caggattcga
    17341 ctggttgttg atcgacggtg agcacacgcc gaataacgtg caaaccgtgc tcacccagct
    17401 acaggcgatt gcgccctacc ccagccagcc ggtagtacgt ccgtcgtgga acgatccggt
    17461 gcaaatcaaa caactgctgg acgtcggcac acaaaccttg ctggtgccga tggtacaaaa
    17521 cgctgacgaa gcccgtgaag cggtacgcgc cacccgttat ccccccgccg gtattcgcgg
    17581 tgtgggcagt gcgctggccc gcgcctcgcg ctggaatcgt attcctgatt acctgcaaaa
    17641 agccaacgat caaatgtgcg tgctggtgca gatcgaaacg cgtgaggcaa tgaagaactt
    17701 accgcagatt ctggacgtgg aaggcgtcga cggcgtgttt atcggcccgg cggatctgag
    17761 cgccgatatg ggttatgccg gtaatccgca gcacccggaa gtacaggccg ccattgagca
    17821 ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg atcctgatcg ccaatgagca
    17881 actggcaaaa cgctatctgg aactgggcgc gctgtttgtc gccgtcggcg ttgacaccac
    17941 cctgctcgcc cgcgccgccg aagcgctggc agcacggttt ggtgcgcagg ccaccgccgt
    18001 gaagcccggc gtgtattaat gccggatgcg ctgcgcttat ccggcctaca ctcgcaccaa
    18061 accgtaggcc ggataaggcg tttacgccgc atccggcaaa aagccgtacc aaatcgcgga
    18121 ccggataagg cgttacgccg catccgccat aaaccttgtc gtaccctaca aaaatcccat
    18181 tagaggaaga aaaatgagcg acacctcacc tgccataccg gagagtatcg atccggcgaa
    18241 tcagcataaa gcgctgactg ccggacaaca ggcggttatt aagaagctat ttcgccgcct
    18301 gagcgtcttt ctgttcgtgc tgtttatctt ctcgttcctt gatcgcatca acatcggctt
    18361 tgccggactc acgatgggac gcgacctcgg tctgagcgcc accatgtttg gcctcgctac
    18421 caccctgttc tatgccgctt atgtcatctt cggcattccc agcaacatta tgctgagtat
    18481 tgtcggtgcg cggcgctgga tcgccaccat catggtgctc tggggcatcg cctctactgc
    18541 caccatgttt gccactggcc ccaccagctt gtacgtactg cgtatactgg ttggcattac
    18601 cgaagccggc tttctgcctg gtattctgct gtatttaacc ttctggtttc cggcctattt
    18661 ccgcgcccgt gccaacgcct tgtttatggt agcaatgccg gtaacgacag cgttgggctc
    18721 gatcgtttcc ggctacattt tgtcgctgga tggcgtcatg gcattaaaag gctggcagtg
    18781 gctgtttttg ctggaaggct tcccgtcggt attactcggc gtcatggtgt ggttctggct
    18841 tgatgactca ccggacaaag ctaagtggct gacgaaagaa gacaaaaaat gcctgcaaga
    18901 gatgatggat aacgatcgtc tgacgctggt tcagccagag ggagccatca gccaccacgc
    18961 catgcaacaa cgcagtatgt ggcgggagat cttcactccg gtggtgatga tgtataccct
    19021 ggcgtatttc tgcctgacca acacacttag tgcgatcagc atctggacac cgcagatcct
    19081 gcaaagtttt aatcagggca gcagtaatat caccatcggc atgctggccg ccgtaccgca
    19141 gatttgtacc attctcggga tggtctactg gagccgtcac tcagatcgcc gccaggaacg
    19201 aaggcatcac accgcccttc cttatttgtt cgctgccgca ggttggttac tggcttcggc
    19261 aactgatcac aacatgatcc agatgctggg gatcattatg gcttcgaccg gatcattcag
    19321 cgcaatggcg attttctgga caacaccgga tcagtccatc agcctgcggg cacgagcgat
    19381 cggtattgcg gtgatcaacg ccactggcaa cattggttca gcgttaagtc cgtttatgat
    19441 cggctggttg aaagatttga ccggcagctt taacagtgga ttgtggtttg ttgccgcgct
    19501 gctggtgatt ggtgcgggga ttatctgggc gattccaatg cagtcctccc gtccgcgagc
    19561 gaccctgtaa ggaacgacga tgtgtgaccg tcagattgcc aatattgata tcagcaaaga
    19621 gtacgatgaa agcctgggca cggacgatgt gcattatcag tcgttcgccc gcatggcggc
    19681 attttttggc cgccatatgc tgccacatcg ccacgaacag tactttcaga tgcatttcct
    19741 caatagcgga cagattgagc tacagcttga cgatcatcgc tactcggtgg aagcgcccct
    19801 gtttgtcctg acgccgccgt ca
//
LOCUS       DAZOZY010000065        19542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZOZY010000065 DAZOZY010000000
VERSION     DAZOZY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.065"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHURQ_001994"
     CDS             complement(<1..346)
                     /locus_tag="ACHURQ_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR0823773.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIV"
     gene            complement(433..2730)
                     /locus_tag="ACHURQ_001995"
     CDS             complement(433..2730)
                     /locus_tag="ACHURQ_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR0823774.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(2720..2995)
                     /locus_tag="ACHURQ_001996"
     CDS             complement(2720..2995)
                     /locus_tag="ACHURQ_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR0823775.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(2992..3216)
                     /locus_tag="ACHURQ_001997"
     CDS             complement(2992..3216)
                     /locus_tag="ACHURQ_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR0823776.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(3219..3518)
                     /locus_tag="ACHURQ_001998"
     CDS             complement(3219..3518)
                     /locus_tag="ACHURQ_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR0823777.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(3518..3742)
                     /locus_tag="ACHURQ_001999"
     CDS             complement(3518..3742)
                     /locus_tag="ACHURQ_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR0823778.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(3806..4306)
                     /locus_tag="ACHURQ_002000"
     CDS             complement(3806..4306)
                     /locus_tag="ACHURQ_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR0823779.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(4476..4748)
                     /locus_tag="ACHURQ_002001"
     CDS             complement(4476..4748)
                     /locus_tag="ACHURQ_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR0823780.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            4885..5178
                     /locus_tag="ACHURQ_002002"
     CDS             4885..5178
                     /locus_tag="ACHURQ_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0823781.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            5248..6228
                     /locus_tag="ACHURQ_002003"
     CDS             5248..6228
                     /locus_tag="ACHURQ_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0823782.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(6414..6914)
                     /gene="cpxP"
                     /locus_tag="ACHURQ_002004"
     CDS             complement(6414..6914)
                     /gene="cpxP"
                     /locus_tag="ACHURQ_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR0823783.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            7064..7762
                     /gene="cpxR"
                     /locus_tag="ACHURQ_002005"
     CDS             7064..7762
                     /gene="cpxR"
                     /locus_tag="ACHURQ_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR0823784.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            7759..9132
                     /gene="cpxA"
                     /locus_tag="ACHURQ_002006"
     CDS             7759..9132
                     /gene="cpxA"
                     /locus_tag="ACHURQ_002006"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR0823785.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(9238..9912)
                     /gene="yiiM"
                     /locus_tag="ACHURQ_002007"
     CDS             complement(9238..9912)
                     /gene="yiiM"
                     /locus_tag="ACHURQ_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR0823786.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(10061..11044)
                     /gene="kdgT"
                     /locus_tag="ACHURQ_002008"
     CDS             complement(10061..11044)
                     /gene="kdgT"
                     /locus_tag="ACHURQ_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR0823787.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(11304..11924)
                     /gene="sodA"
                     /locus_tag="ACHURQ_002009"
     CDS             complement(11304..11924)
                     /gene="sodA"
                     /locus_tag="ACHURQ_002009"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR0823788.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            12209..13243
                     /gene="rhaT"
                     /locus_tag="ACHURQ_002010"
     CDS             12209..13243
                     /gene="rhaT"
                     /locus_tag="ACHURQ_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR0823789.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(13240..14088)
                     /gene="rhaR"
                     /locus_tag="ACHURQ_002011"
     CDS             complement(13240..14088)
                     /gene="rhaR"
                     /locus_tag="ACHURQ_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR0823790.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(14162..14998)
                     /gene="rhaS"
                     /locus_tag="ACHURQ_002012"
     CDS             complement(14162..14998)
                     /gene="rhaS"
                     /locus_tag="ACHURQ_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR0823791.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            15286..16755
                     /gene="rhaB"
                     /locus_tag="ACHURQ_002013"
     CDS             15286..16755
                     /gene="rhaB"
                     /locus_tag="ACHURQ_002013"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR0823792.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            16752..18011
                     /gene="rhaA"
                     /locus_tag="ACHURQ_002014"
     CDS             16752..18011
                     /gene="rhaA"
                     /locus_tag="ACHURQ_002014"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR0823793.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            18253..19077
                     /gene="rhaD"
                     /locus_tag="ACHURQ_002015"
     CDS             18253..19077
                     /gene="rhaD"
                     /locus_tag="ACHURQ_002015"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR0823794.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            19087..19401
                     /gene="rhaM"
                     /locus_tag="ACHURQ_002016"
     CDS             19087..19401
                     /gene="rhaM"
                     /locus_tag="ACHURQ_002016"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR0823795.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         4861 a         5055 c         4880 g         4746 t
ORIGIN      
        1 ttacgatttc tggagatact tcgcgaatta aacgttcgaa ctcataaaga agaggccatt
       61 ttttatcatc agccttatct tttccttgag tatattttga aaaaggttgg caaggtgcgc
      121 atcctgccaa tacttttaca gacgcatcac cataaagcct cagcagttcc tcttttgtta
      181 ctttagcgat atcttgctca ataaacttag atttattgtt tctttcatag ggaaacctac
      241 attccccctc aaggtcaatg cctgcaacga catcaagccc cgcaagctgc aaaccatgag
      301 ttaatccgcc agcaccacaa aaaaggtcta tcacctttac agtcatcact cactccaaat
      361 ctatacgata ctcagtatac atccctttct acaaaatctc gaggcctaac ttataaactc
      421 atatttattg agttaacgct tcactgagtt tgactctaat actgttgaga atcccgacca
      481 ctcatcagcg agcgggtaag tgaatttttt cccgtcataa tttacggttg ccccacgcgc
      541 cagcacctca agctcccatc gctgcggcct gataccgttc tgagcaaggt caacgcggat
      601 acgggtaatt tgcattcgtt ccgaccgggt cagtctggcc gatggcgcta tttcatgcgg
      661 ttttaacggg cttccgtttc tttgctgacg gtttggtctt ctcagtccgt gttttaatgc
      721 acctctgagc gccctcacaa cctccgggtc attccattcg ataacaccgt catctaccag
      781 attaagcact gctgcggcgt gctcagaagg tgtgggagcc ggtaacgaag tatcaccacc
      841 agtgagcttt ccacagttat tgacaggact ccgaggcgcg gcgatgccgc tttttaaagt
      901 caaaggctca acgaccggca ctttcggaac aatgcgccag tccgtcgttc tggtgatatg
      961 aatatgacgc gcgccgagat gcggcgcgta aatgccgacc actctctcga cttcttcctc
     1021 gtactcgtta acttcatccg acggactacg ggcgaccctg acagtctgac aatcgcgcgg
     1081 gacatttgcc ccaccctgcg cgctgatata cagcgcaaaa tcaccactgt ctgcggcggc
     1141 gcgtgcagcc tcgacgcgct cgtcaaactc atcagcaatg ctgacgccgc gaggcaattt
     1201 gcgtagttca cggtaagccc ccattgtcgg caggccaacc gttttaaatt gcggaatgcg
     1261 ccacgttgac gcccatgcgg taacagccgc ggcagtatct ttcagcggtc tgccgctatc
     1321 gttatcgagc tgcccatcca gtgcatagcc gtcgatattt tttgagatgt atttcgcgat
     1381 ataccccgca gcaccgcccc gattaagatg ttttgcctga aaacggtttc gcgcagctcc
     1441 tctttcgtcg ccatcctctt tgagcgcgta gcgacgcatg atttcgataa tctggttacg
     1501 ctggcgtgga ttacaaaaaa gcatcatatg ccagtgcggc gttccgtcgt ggtgtggctc
     1561 gacgactcgc aaaccgtaga cctgtaaatc attatccttg aatgccgtgc gcatcaggct
     1621 ccagatacgg cagagataac gctgcgcatc ctttggatta aatgcctcat cgttccagcc
     1681 gtgatttagc tggacggttt tactttcgcc ttttccaacc tgacgtgtcg ggtgatactt
     1741 tgacggcgcg gtcagcgtga taaacatccc cacatcacct tctgcggcgg cgtaacgctc
     1801 aataccggca atggtgttca tcagctccat ccggcgaatt tcaggattag aaatactgcc
     1861 catcacctta ctgataaggt cgatgcgctc gcctgtttcc ctgttttcga gatcacacga
     1921 tttaagaaat tccagatttg cctggcggcg tgcacgcaca tcacgaatgg catgtttact
     1981 ggcataagga gaacggtctt tattgacctc cccgacagca atcagtaacg cctcatgcca
     2041 gcgcatacgc tggcctttaa gctgactaat ccaccactca tcgttaaaca gacgggcaat
     2101 ggcagaatat gcctgcctcg tggtcatctg tcctttacgg tattttttcc agtagagagg
     2161 ggaaatattg aaagcacgtg cagcgccagc aacatgacca tagaggtgag cctgcgcctc
     2221 atccgtaaac agcgattctt tttcgccatg cgcatccacc caggcatcgc tgagttcctc
     2281 atacatcatg aaaagctgcg atgagatacg ggcggcaaac tttttcagct ccttgtcatt
     2341 cattcccggc aggcgcgcat agtggtcacg ctcagccaga aacagcatcg acgcgtcggt
     2401 gttcatttca tggcgctgat tcacacgctc aatgcgcggc cataaacgac gctgaaaagt
     2461 ggatgtgagg aaataaaacc cgtgcaccgg gcttttattg cgccggatgt agtcatagcg
     2521 tgacgtaaac agcgagcgca aaaagtaagg caggcggtta atcgtggata aaacaccttg
     2581 cacctgacgc atctcgtcac gtgtaagggg tctttcgcgc ccgacagcct cgcgtggcgc
     2641 gttccatgca taagcaccgg taaacgcctt accggtgcct gcagcaaatg ctgacggagg
     2701 gacaaaacgc ccggaggctt taacggccat atgagccaaa agcctctgaa caacgcttgc
     2761 tgagttgctc aacctgcgcg tttaaatcag caaaagactt tgcgcttccg gtcagaatat
     2821 cgtgatgcat caggccggaa acgagctggc ttaatttcgg gtaataacca accaccgcca
     2881 gccattcctg accggcgttt ttaccgcttt ccgctctctt tttctcgtgg agaataaact
     2941 ggaagctgtc actggtaacg acataacgtt cgccaatttc aatacgaata ctcatgccgt
     3001 tctccggtaa tgtttgtttt ttgcttcaaa gactgactga caggaaacac aacgcgtggc
     3061 tgacggataa gccgcacgac gggcagcagg aattggcgcg tcacactctt cgcaaaccag
     3121 cgcagaagca ccgcaatgtt ttacccttgc cgcgttaatc tggcgctcca gtaattcagc
     3181 ctgttgttcc tgaataaaat ctatgttgtc cggcattatc agctcctttt atcgttaagt
     3241 ttcctggata catcagcgca ataactggca agttctgtcg ttaattttgt cagttcatcc
     3301 actgaggaaa tttgcttgtg gaatacagcg cgtttaacaa gtaaattgac cacatcagac
     3361 aggaggttta attcattctg ataaatcgcg ataacagatt cagtgatgtc gcgtttttct
     3421 ttatcaagac aaagttgaat aagagacaag tcgccatttt tcataacggc gatttttaag
     3481 gcgttattca gtaatacaac tgaatgagaa caggacatca aagcacctcc ccgcgagaca
     3541 atccgatatt gtgaaatttt tccgactcct gactgagcag ctcgactatc tccacgcggg
     3601 ataactccgc ctttgtgata tggcgaatca tggcgtcaag atgagaagaa aagcgcgtcg
     3661 cagcgtcggc ctgtgcttcg gttctggcct gttgcagcag taatgcgtat ttaccgcact
     3721 gattttcaga aactgtatgc atgactttct ccaggcaaaa agaagccccg cacaattaag
     3781 tgcgttaaaa actctggtta attatttaat gcaaatattg ctctggtttt accgacgtca
     3841 gaattgtcgg tgcatactca aacagactga atagttcacg taatgcacgg aataaagcat
     3901 cacgccagta acatgactct tcattaattc gccagtatgg ctgattgaat tctttttcag
     3961 tcaatccggc atgcataaat aaagtacgac gctgactgac tgttaaaaaa ctaatatatg
     4021 catactcact tgcgccaacc tgacggcgtt ttgagaatgc cccacgcaat tcatcaattg
     4081 cacaaaccag ccgttcacgt tcgacgtcgt tcatttcttc aaaacgcatc gttgcgtgac
     4141 gttgttttaa ctgcgcatga aagcaaactg ttagccgttc gcgctccatc atctgattat
     4201 aataatcaca tgtatcctgc caacgaggga cggcaagatg cttaccaatt atccggcgca
     4261 tagctgctgg ctgtttttcg acgagattga gcgtcatcac tgtcatttcc agaccctccg
     4321 gcttttcaga aaggtcagag ccttttttaa cggactctgt tttttggtgc ggataatgat
     4381 tcccttgcga cccttcccgt gggtgatggt gaagtcaatc gccctagggc tttcgttacg
     4441 caataactga gcaatacaac gcggctcatt cataatcaca accccatcca caaaagccat
     4501 gcatcacgct gttcaaccgg tcggttataa aacgcctcac gtacagcgcg attaaactca
     4561 ggaatgaaaa cccatttttc accggcacga gccttcggtt tgcaaggatc acgcaattca
     4621 ataattggta atttatttgc cttcaccatt tcactgacgg ctgtctttgg cttccctaat
     4681 aaatcagcaa atttatccac atgaaccgca tcaagcggat actgaatcac ataattttca
     4741 gcgtccatat atggtaccct cataggatcc agccctttct aaaccactca aaaccgttta
     4801 gacgctggtt tattctcaaa tcaatggaac ctatataggt tccagttttg agggaattta
     4861 gtccctatat aggcaccatg tcaaatgaaa ttaagcgaaa agattaaggc cttgcgtgag
     4921 gctgaagggc taagccaatc aaaattctgt gaaatcatag agttaccgct aagcacactt
     4981 aaaaaatatg aaggaggaaa ctttgaaccc ggtggcacag ctttgctaaa aatcactatg
     5041 catcccacat tccaaaaata tgctctatgg cttatgacag ataaaaccgc gccggacgca
     5101 ggacaaatcg caccggctct cgcgcacatt gggccagagt caacagagtc caaccactcc
     5161 gcgaaaagga ttggctaact ctatataaag attacatttt caccatttgc taccaagatg
     5221 gtgaatacag cgccggaggg ctttcttatg gcaattaaga agctcgatga tggtcgctat
     5281 gaagtggaca ttagacctcg cggtcgcgac ggaaaacgca tccgcaggaa attcgaaaga
     5341 aaagctgaag cactagcatt tgagcgatac acaatcgcca atgccagtca gaaagaatgg
     5401 ggaggccagc gagcagaccg ccggactttg agtgagttgc tggacatctg gtggaaatat
     5461 cacgggcaaa accacgagca tggaacaaaa gagtttaatc atctactcaa aaccatcagc
     5521 ggcataggtg atataccagt gagcaggatg agcaaaaggg ctttgatgga ttatcgttcc
     5581 atgcgactac gtgatggtat cagtgccgca acgataaacc gtgacatgta cagattatcc
     5641 ggcatgttca caaaattaat tcaattggat gaattttccg ggcaacaccc aattcacgga
     5701 ctgccgccac tggcggaggc caaccctgaa atgacgttcc tggaaaaagc agaaatcgaa
     5761 aaactgttaa atgttttggc tggtgatgac ttacttgtcg cgcttttatg tctgagcact
     5821 ggaggaagat ggacgaaagt tgccacgcta aaaccagcac agattacaaa ttgcagggtt
     5881 accttcctga aaaccaaaaa cggtaaaaag cgaaccgtgc cgatttctga ggaactggag
     5941 aaaaaagtta aagaggaggc cagcgccaaa ttattcaaag ttgattatga gaaattttgc
     6001 gggattttac gcagagtgaa acctgatata ccacccaatc aggcaaccca catcctgcgg
     6061 catacattcg caagccattt catgatgaat gggggcaata taatcgcact gcaacagatt
     6121 ctgggacatg cgagcattca gcagacgatg gcctatgcgc accttgcgcc tgactatctg
     6181 caaaatgccg tcgctctgaa tccactaaaa ggcggagtga cgttataaat ttccccctct
     6241 gagtgtccac atagtgtcca cactcaagaa attttgaaac cgctccaaac ccttacagct
     6301 ttcgataagt aactgttttt acttttgatt gcatgtaagt atttgataaa aaaaacccca
     6361 catcatgtgg gggaagacag ggatggtgtc tatggcaagg aaaacagggt ttactactgg
     6421 gaacgtgagt tgctactact caatagcttc aacgatgaac ttttttgcca ttgcgtcacg
     6481 tcacgcaact gctccattcg ttgttgatgt ttctcgttta aaaccgcttg ctgctccggc
     6541 gttaacaggc gatacatttg gttgcggact tttgccatct caacctgacg agcaatttgc
     6601 tcattcgcca ttttttctgc ctgtgcgcgc acagcgtttt catcaaaatt ttctgcggtg
     6661 acaaggcgat gcattgtctc cagttcgcta acattaacag gaggctgttc gtgccgggcc
     6721 tgttgcataa gatctcgcat ctgctgacgc tgatgttcgg ttaaacttat gccgtcgaac
     6781 atatggctct gcgtactgcg ctgcgtaagt tcttcacccg gatgccagtt atcgcctgaa
     6841 ccgacttcag cagcgtggct taatgaactg actgccagcg ttgaggccat gacggcagcg
     6901 gtaactatgc gcatcatttg ctcccaaaat ctttctgtcg cgattcaacg atagagagtt
     6961 tacgattcag gctgcaaaca tgcgtcaggg ggtgtaaaac aacgtaaagt catggattag
     7021 cgacgtctga tgacgtaatt tctgcctcgg aggtatttaa acaatgaata aaatcctgtt
     7081 agttgatgat gaccgagagc tgacttccct attaaaggag ctgctcgaga tggaaggctt
     7141 caacgtgatt gttgcccacg atggggaaca ggcgcttgat cttctggacg acagcattga
     7201 tttacttttg cttgacgtaa tgatgccgaa gaaaaatggt atcgacacat taaaagcact
     7261 tcgccagaca caccagacgc ctgtcattat gttgacggcg cgcggcagtg aacttgatcg
     7321 cgttctcggc cttgagctgg gcgcagatga ctatctcccg aaaccgttta atgatcgtga
     7381 gctggtggca cgtattcgcg cgatcctgcg ccgttcgcac tggagcgagc aacagcaaaa
     7441 caacgacaac ggttcaccga cactggaagt tgatgcctta gtgctgaatc caggccgtca
     7501 ggaagccagc ttcgacgggc aaacgctgga gttaaccggt actgagttta ccctgctcta
     7561 tttgctggca cagcatctgg gtcaggtggt ttcccgtgaa catttaagcc aggaagtgtt
     7621 gggcaaacgc ctgacgcctt tcgaccgcgc tattgatatg cacatttcca acctgcgtcg
     7681 taaactgccg gatcgtaaag atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta
     7741 tctgatggtt tctgcttcat gataggcagc ttaaccgcgc gcatcttcgc catcttctgg
     7801 ctgacgctgg cgctggtgtt gatgttggtt ttgatgttac ccaagctcga ttcacgccag
     7861 atgaccgagc ttctggatag cgaacagcgt caggggctga tgattgagca gcatgtcgaa
     7921 gcggagctgg cgaacgatcc gcccaacgat ttaatgtggt ggcggcgtct gttccgggcg
     7981 attgataagt gggcaccgcc aggacagcgt ttgttattgg tgaccaccga aggccgcgtg
     8041 atcggcgctg aacgcagcga aatgcagatc attcgtaact ttattggtca ggccgataac
     8101 gccgatcatc cgcagaagaa aaagtatggc cgcgtggaac tggtcggtcc gttctccgtg
     8161 cgtgatggcg aagataatta ccaactttat ctgattcgtc cggccagcag ttctcaatcc
     8221 gatttcatta acttactgtt tgaccgcccg ctattactgc tgattgtcac catgttggtc
     8281 agtacgccgc tgctgttgtg gttggcctgg agtctggcaa aaccggcgcg taagctgaaa
     8341 aacgctgccg atgaagttgc ccagggaaac ttacgccagc acccggaact ggaagcgggg
     8401 ccacaggaat tccttgccgc aggtgccagt tttaaccaga tggtcaccgc gctggagcgc
     8461 atgatgacct ctcagcagcg tctgctttct gatatctctc acgagctgcg caccccgctg
     8521 acgcgtctgc aactgggtac ggcgttactg cgccgtcgta gcggtgaaag caaggaactc
     8581 gagcgtattg aaaccgaagc gcaacgtctg gacagcatga tcaacgatct gttggtgatg
     8641 tcacgtaatc agcaaaaaaa cgcgctggtt agcgaaacca tcaaagccaa ccagttgtgg
     8701 agtgaagtgc tggataacgc ggcgttcgaa gccgagcaaa tgggcaagtc gttgacagtt
     8761 aacttcccgc ctgggccgtg gccgctgtac ggcaatccga acgccctgga aagtgcgctg
     8821 gaaaacattg ttcgtaatgc tctgcgttat tcccatacga agattgaagt gggctttgcg
     8881 gtagataaag acggtatcac cattacggtg gacgacgatg gtcctggcgt tagcccggaa
     8941 gatcgcgaac agattttccg tccgttctat cggaccgatg aagcacgcga tcgtgaatct
     9001 ggcggtacag gattggggct ggcgattgtt gaaaccgcca ttcagcagca tcgtggctgg
     9061 gtgaaggcag aagacagccc gctgggcggt ttacggctgg tgatttggtt gccgctgtat
     9121 aagcggagtt aaactccgca tttgtaggca ggataaggcg tttacgccgc atccggcatt
     9181 tgagcaggat gcctgatgcg acgctgatag cgtcttatca ggcctacact ccgggggtta
     9241 ttttccccac aaccggcggg aaaaatcttc gatcttgccg ctcagtcggc gcttttgcat
     9301 cgtccgcgtc cagcttttcg ataacccggc ggcggagagt aaacggtgat actgatcgtc
     9361 atcaaacggc atatgccatg cgatggcggc agcctcctgc acggtcacat cgctgacacg
     9421 ggaaaccaac tccagcggcg cgtccgcaga cactttccct ggcgctatca cgctatacaa
     9481 ccaccccacc ttacctgtgt tttgcatcaa ctgcgctata tcgctgatat caaaatggta
     9541 attgagttta tagcaaggcg agcgcggctg gctgacctga attaatgcct ctccccagcg
     9601 gaaaatatcg cccatatgaa cattactttc cgtgagaccg tcggttgaga gattttcgcc
     9661 aaacgcaggc gcaacaaaca actccgcctg ttccggaaat tcccgcaccc agtagagata
     9721 gtgctcacga ggataatgac acagcgctct gtctggcccg ccgtgaacct ttttctccgc
     9781 ctgctcgtca ccttccagcc ccagctccgt cagcatcaac tcaccatcaa cctggatttt
     9841 ggcaattgcg ctgggtttgc cttcgggata agcctgaatc ttgcctgtgt atacatcaac
     9901 cggatatcgc atctgttccc tctccacgag aaatttcccc attagaccat atttatttta
     9961 tgtcaggcgt gctgttttcg cggtaagtat ccaggcagga actcaatagc aaaccggacg
    10021 aacgttagtt catccggctg tggggattaa ggaagcaggt ttatttcacc gtacctaaaa
    10081 tttcgatttt cgctgctctg gctttgactt tacgtgacca gatagaggta agaattggca
    10141 ccagaatcga agtcacaatg accgccgtcg ctaccagcga agttgctgcc ggagccatcg
    10201 gtttaaacgc aggcaccatt tctgcaatca gcacaggtgt cgctaccgcg gcccctgcgg
    10261 aactggaagc ggcaattccg gcagtgccat cgccaccgcc aatcaattta tcggcgataa
    10321 tcaacggaat accggtcacg ataattactg ccacacccaa caggatcccc agcaaaccag
    10381 tctgggcaat cacagtcaaa tcaatggtat tgcccagcgc gaaggcaaag aatggaatca
    10441 gcgtttgcac tgctttgctg aaaaattctc gtaattcagg gtcaagattc cccagggcaa
    10501 agcccaccag gaacggtaat actgcgccga cgaaaacatg cggttcaaac gaggcaatcc
    10561 cggcagtgcc cagaataatc atcgtcatga gcggcccgga ctccaacgac atcaacacaa
    10621 atgccccagc ttcttctttc gtaccgtact gctgcatgat ggaagcgtaa agtccgccgt
    10681 tggtcatatc catcgccgcc accagcgcca gcgttgaaag tccggcaaag aatccaactt
    10741 caacaccatg ttccggaata atgcgcgagg caattgccgc aaccacccac gcgacggcaa
    10801 ttttagttac caccagcgta ccggatttac gcagtaccgt tcccgtcgcg cttaatttta
    10861 ttgacgcccc catgcaaaaa aaccacaccg ccagaatggg caccgtaccg gtaatcatcc
    10921 cgttggtgaa tgatccaaaa tatttccccg cccccggcga gaaggtgtgg cacagtgcgc
    10981 caaggaatag cgggacgagc atcatccccc ccgggatttt ctcaatcgag cgttttatct
    11041 gcatttccat cacctgtcgg catattttta agggctgatg cgtaattcct caatggagca
    11101 tcagtgttga tggaaacgaa gatagacatt catttaatca ataaaagtga tcaaaaccac
    11161 atcaattgaa acgctgtttt atttttatcg gatcattgtt tttattttga aattgatcat
    11221 aaaaaaacca ccgccgttgg cgatggttca ttttttaagc tgatatgcgg cctatacgcc
    11281 tcattgcagc aggcggcaaa tgattatttt ttcgccgcaa aacgtgccgc tgcttcgtcc
    11341 cagttcacca cgttccagaa ctctttaatg tagtccggac ggcggttctg gaatttcagg
    11401 tagtaagcat gttcccacac atccaggccc agaatcggga agccggaagc gccagaaata
    11461 gcttcaccca tcagcggaga atcctggtta gcagtagaaa ccaccgccag tttatcgcct
    11521 ttcagcacca gccatgccca gccggaacca aagcgggaag ctgccgcttt ttcaaattct
    11581 gctttgaagt tatcaacgga gccgaagtca cgttcgatag ccgctttcag gtcaccctgc
    11641 agggtggtgc cttttttcag acctttccag aacaggctgt ggttagcgtg accgccagcg
    11701 ttgttgcgca gtacggtttt cttgtctgct ggcagctggt ccagtttggt gatcagctct
    11761 tcaaccggca ggttggcaaa ttctggcagg ctttccagcg ccgcgttggc gttgtttacg
    11821 taggtctgat ggtgtttggt gtggtggatt tccatggtct gcttatcgaa gtgcggttcc
    11881 agggcatcgt aagcatacgg cagggatggc agggtatagc tcatattcat ctccagtatt
    11941 gtcgggcggc cgattgttaa tgccgcgtaa gcagttggtt cattatagtt aattaaatga
    12001 tattgaaaat gattatcaat gccgtacttt tcgtaagggt atggttttgc aggaaaatgc
    12061 ccgagatgtg aagcaaatca cccacttaat gccgtgattg ccagtaaatc gacaacggcg
    12121 gcaacaggcg aaaggttaat cgacagcacg atttttacac tcatctcgtc ggagatgtga
    12181 agcgacgaaa aatgatgagg ataagaagat gagtaacgcg attacgatgg ggatattttg
    12241 gcatttgatc ggcgcggcca gtgcagcctg tttttacgct ccgttcaaaa aagtaaaaaa
    12301 atggtcatgg gaaaccatgt ggtcagtcgg tgggattgtt tcgtggatta ttctgccgtg
    12361 gaccatcagc gccctgttac taccggattt ctgggcgtat tacagctcgt ttagtctctc
    12421 tacgctgctg cctgtttttc tgttcggcgc tatgtggggg atcggtaata tcaactatgg
    12481 cctgaccatg cgttatctcg gcatgtcgat gggaattggc atcgccattg gcattacgtt
    12541 gattgtcggt acgctgatga cgccaattat caacggcaat ttcgatgtgc tgattaacac
    12601 cgaaggcgga cgcatgacgt tgctcggcgt tctggtggcg ctgattggcg tagggattgt
    12661 aactcgagcc gggcagttga aagagcgcaa gatgggcatt aaagccgaag agttcaatct
    12721 gaaaaaaggg ctggtgctgg cggtgatgtg cggcattttc tctgccggga tgtcctttgc
    12781 gatgaacgcc gcaaaaccga tgcatgaagc cgctgccgca cttggcgtcg atccactgta
    12841 tgtcgctctg ccaagctatg ttgtcatcat gggcggcggc gcgatcatta acctcggttt
    12901 ctgttttatt cgtctggcaa aagtgaagga tttgtcgcta aaagccgact tctcgctggc
    12961 aaaaccgctg atcattcaca atgtgttact ctcggcactg ggcgggttga tgtggtatct
    13021 gcaattcttt ttctatgcct ggggccacgc ccgcattccg gcgcagtatg actacatcag
    13081 ttggatgctg catatgagtt tctatgtatt gtgcggcggt atcgtcgggc tggtgctgaa
    13141 agagtggaac aatacaggac gccgtccggt aacggtgttg agcctcggtt gtgtggtgat
    13201 tattgtcgcc gctaacatcg tcggcatcgg catggcgaat taatctttct gcgaattgag
    13261 atgacgccac tggctgggcg tcatcccggt ttcccgggta aacaccaccg aaaaatagtt
    13321 actatcttca aagccacatt cggtcgaaat atcactgatt aacaggcggc tatgctggag
    13381 aagatattgc gcatgacaca ctctgacctg tcgcagatat tgattgatgg tcattccagt
    13441 ctgctggcga aattgctgac gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt
    13501 atccagcgca aagggacttt tcaggctagc cgccaaccgg gtaatcagct tatccagcaa
    13561 cgtttcgctg gatgttggcg gcaacgaatc actggtgtaa cgatggcgat tcagcaacat
    13621 caccaactgc ccgaacagca actcagccat ttcgttagca aacgacacat gctgactact
    13681 ttcatgctca agctgaccga taacctgccg cgcctgcgcc atccccacgc tacctaagcg
    13741 ccagtgtggt tgccctgcgc tggcgctaaa tcccggaatc gctccctgcc agtcaagatt
    13801 cagcttcagc cgctccgggc aataaataat attctgcaaa accagatcgt taacggaagc
    13861 gtaggagtgt ttatcgtcag catgaatata aaagagatcg ccacgggtaa tgcgataagg
    13921 acgatcgttg agtacatgca gaccattacc gcgccagaca atcaccagct cacaaaaatc
    13981 atgtgtatgt tcggcaaaga catcttgcgg ataacggtca gccacagcga ctgcctgctg
    14041 gtcgctggca aaaaaatcat ctttgagaag ttttaactga tgcgccaccg tggctacctc
    14101 ggccagagaa cgaagttgat tattcgcaat atggcgtaca aatacgttga gaagattcgc
    14161 gttattgcag aaagccatcc cgtccctggc gaatatcacg cggtgaccag ttaaactctc
    14221 ggcgaaaaag cgtcgaaaag tggttactgt cgctgaatcc acagcgatag gcgatgtcag
    14281 taacgctggc ctcgctgtgg cgtagcagat gtcgggcttt catcagtcgc aggcggttca
    14341 ggtatcgctg aggcgtcagt cccgtttgct gcttaagctg ccgatgtagc gtacgcagtg
    14401 aaagagaaaa ttgatccgcc acggcatccc aattcacctc atcggcaaaa tggtcctcca
    14461 gccaggccag aagcaagttg agacgtgatg cgctgttttc caggttctcc tgcaaactgc
    14521 ttttacgcag caagagcagt aattgcataa acaagatctc gcgactggcg gtcgagggta
    14581 aatcattttc cccttcctgc tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata
    14641 cgctgtggtt aacgcgccag tgagacggat actgcccatc cagctcttgt ggcagcaact
    14701 gattcagccc ggcgagaaac tgaaatcgat ccggcgagcg atacagcaca ttggtcagac
    14761 acagattatc ggtatgttca tacagatgcc gatcatgagc gcgtacgaaa cagaccgtgc
    14821 caccggtgat ggtatagggc tgcccattaa acacatgaat acccgtgcca tgttcgacaa
    14881 tcacaatttc atgaaaatca tgatgatgtt caggaaaatc cgcctgcggg agccggggtt
    14941 ctatcgccac ggacgcgtta ccagacggaa aaaaatccac actatgtaat acggtcatac
    15001 tggcctcctg atgtcgtcaa cacggcgaaa tagtaatcac gaggtcaggt tcttacctta
    15061 aattttcgac ggaaaaccac gtaaaaaacg tcgatttttc aagatacagc gtgaattttc
    15121 aggaaatgcg gtgagcatca catcaccaca atgaagcaaa ttgtgaacat catcacgttc
    15181 atctttccct ggttgccaat ggcccatttt cctgtcagta acgagaaggt cacgaattca
    15241 ggcgcttttt agactggtcg taatgaaatt cagcaggatc acattatgac ctttcgcaat
    15301 tgtgtcgccg tcgatctcgg cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt
    15361 gaatgccgca gcctgacgct gcgcgaaatc catcgtttta acaatggcct gcatagccag
    15421 aacggttatg tcacctggaa tgtggatagc ctggaaagtg ccattcgcct tggattaaac
    15481 aaggtgtgcg aggaagggat tcgtatcgat agcattggga ttgatacctg gggcgtggac
    15541 tttgtgctgc tcgaccaaca gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc
    15601 cgctccaatg gcctaatggc gcaggcacag caacaactcg gcaaacgcga tatttatcaa
    15661 cgtagcggca tccagtttct gcccttcaat acgctttatc aactgcgtgc gctgacggag
    15721 caacaacctg aacttattcc acacattgct cacgctctgc tgatgccgga ttacttcagc
    15781 tatcgcctga ccggcaagat gaactgggag tacaccaacg ccacgaccac gcaactggtc
    15841 aatatcaata gcgacgactg ggacgagtcg ctactggcgt ggagcggggc taacaaagcc
    15901 tggtttggtc gcccgacgca tccgggtaat gtcattgggc actggatttg cccgcagggt
    15961 aatgagattc cggtggtcgc cgttgccagc catgataccg ccagcgcggt tatcgcctcg
    16021 ccgttaaacg gttcacgcgc tgcttatctc tcttctggca cctggtcatt gatgggcttc
    16081 gaaagccaga cgccatttac caatgacacg gcgctggcag ccaacataac caatgaaggc
    16141 ggggcggaag gtcgctatcg ggtgctgaaa aatattatgg gcttatggct gcttcagcga
    16201 gtgcttcagg agcggcaaat caacgatctc ccggcgctta tcgccgcgac acaggcactt
    16261 ccggcctgcc gcttcatcat caatcccaat gacgatcgct ttattaatcc tgaggcgatg
    16321 tgcagcgaaa ttcaggctgc gtgtcgggaa atggcgcaac cgctcccgga aagtgatgct
    16381 gaactggcgc gctgcatttt cgacagtctg gcgctgctgt atgccgatgt gttgcatgag
    16441 ctggcgcagc tacgcggtga agatttctcg caactgcata ttgtcggcgg cggctgccag
    16501 aacacgctgc tcaaccagct atgtgccgat gcctgcggta ttcgggtgat cgccgggcct
    16561 gttgaagcct cgacgctcgg caatatcggc atccagttaa tgacgctgga tgaactcaac
    16621 aatgtggatg atttccgtca ggtcgtcagc accaccgcga atctgaccac ctttacccct
    16681 aatcctgaca gtgaaattgc ccactatgtg gcgcagcttc actcgacacg acagacaaag
    16741 gagctttgcg catgaccact caactggaac aggcctggga gctggcgaaa cagcgtttcg
    16801 cggcggtggg gattgatgtc gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa
    16861 tgcactgctg gcagggcgat gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg
    16921 gtgggattca ggctactggt aattatccgg gcaaagcgcg taatgccagt gagctacgag
    16981 ccgatctgga acaggctatg cggctgattc cggggccgaa acggcttaat ttacatgcca
    17041 tctatctgga atcagacacg ccagtctcgc gcgaccagat caaacctgag cacttcaaaa
    17101 actgggttga atgggcgaaa gccaatcagc tcggtctgga ttttaacccc tcctgtttct
    17161 cgcatccgct aagcgccgat ggctttacgc tctcgcatcc cgacgacagc attcgccagt
    17221 tctggattga tcactgcaaa gccagccgtc gcgtttcggc ctattttggc gagcaactcg
    17281 gcacaccatc ggtgatgaac atctggatcc cggatggtat gaaagatatc accgttgacc
    17341 gtctcgcccc gcgtcagcgt ctgctggcag cactggatga ggtgatcagc gagaagctgg
    17401 atccggcgca ccatatcgac gccgttgaga gcaaattgtt tggcattggc gcagagagct
    17461 acacggttgg ctccaatgag ttttacatgg ggtatgccac cagccgccag actgcgctgt
    17521 gcctggacgc cgggcatttc cacccgactg aagtgatttc cgacaagatt tccgccgcca
    17581 tgctgtatgt accgcagttg ctgctgcacg tcagccgtcc ggttcgctgg gacagcgatc
    17641 acgtggtgct gctggatgat gaaacccagg cgattgccag tgagattgtt cgtcacgatc
    17701 tgttcgaccg ggtgcatatc ggcctcgact tctttgatgc ctctatcaac cgcattgccg
    17761 cgtgggtcat tggtacacgc aatatgaaaa aagccctgct gcgtgcgttg ctggaaccta
    17821 ccgctgagct gcgcaagctg gaagcggcgg gcgattacac tgcgcgtctg gcgctactgg
    17881 aagaacagaa atcgttgccg tggcaggcgg tctgggaaat gtattgccaa cgtcacgata
    17941 cgccagcagg tagcgaatgg ctggagagcg tgcgggctta tgagaaagcg attttgagcc
    18001 agcgtgggta aacactgccg gatgcggcgc gagcgcctta tccggcctac aggtcgaatg
    18061 accatgcgtg ctgccaccct caccccggcc ctctccctga aagggcgagg gagaaaaaca
    18121 tagcctcgtg cggcaacgga gctgaacacc gctccggaca gtcccctcgc ccctacgggg
    18181 agagggttag ggtgagggga acaagccgca ccatgtctaa acaaggcaac ctaatttaca
    18241 ggaacacaga acatgcaaaa cattacccag tcctggtttg tccagggaat gatcaaagcc
    18301 accaccgatg cctggctgaa aggctgggac gagcgcaatg gtggtaacct gacgctgcgc
    18361 ctggatgacg ccgatatcgc gccttatcac ggcaactttc atgcacaacc gcgctatatt
    18421 cccctcagcc aacccatgcc tttactggca aatacgccgt ttattgtcac cggctccggc
    18481 aaattcttcc gtaacgtcca gcttgatcct gcagctaact taggcgtcgt aaaagtcgac
    18541 agcgacggcg cgggctacca tattctctgg gggttaacca acgaagccgt tccaacttcc
    18601 gaacttccgg ctcacttcct ttcccactgc gagcgcatta aagccaccaa cggtaaagat
    18661 cgggtgatca tgcactgcca cgccaccaac ctgatcgccc tcacctatgt acttgaaaac
    18721 gacaccgcgg tctttactcg ccaactgtgg gaaggcagca ccgagtgtct ggtggtattc
    18781 ccggatggcg ttggcattct gccgtggatg gtgcccggca cggacgaaat cggccaggcg
    18841 accgcacaag agatgcaaaa acattcgctg gtgttgtggc ccttccacgg cgtcttcggc
    18901 agcgggtcga cgttggatga aacattcggt ttaatcgaca ccgcagaaaa atcagcacaa
    18961 gtattagtga aggtttattc gatgggcggc atgaaacaga ccatcagccg tgaagagttg
    19021 atagcgctcg gcaagcgctt cggcgttaca ccactagcta gtgcgctggc gctgtaagga
    19081 gcaaacatga tccgcaaagc ctttgtcatg caggttaacc ccgacgccca cgaagagtat
    19141 cagcgtcggc ataaccccat ctggccggaa ctggaagcag tgttgaaatc tcacggtgcg
    19201 cataactacg ccatttacct cgacaaagcg cgtaatctgc tgtttgccac ggtagagatt
    19261 gaatctgaag aacggtggaa tgcggtagcc agcactgatg tttgccaacg ttggtggaaa
    19321 tatatgaccg atgttatgcc cgctaacccg gataacagcc cggtgagtag cgagctgcaa
    19381 gaagtgtttt acctgccgta attgcccaat gtgccagaga tgcctccggc acaatatttt
    19441 aaatttcgat ttgaaaaatt aaacatatta catctcccgg taagttatat ttccctgata
    19501 cattgtgagt aaatcacaaa aataatgaat aacccattaa tg
//
LOCUS       DAZOZY010000066        19530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZOZY010000066 DAZOZY010000000
VERSION     DAZOZY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.066"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHURQ_002017"
     CDS             <1..245
                     /locus_tag="ACHURQ_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR0823796.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHURQ_002018"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHURQ_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR0823797.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHURQ_002019"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHURQ_002019"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR0823798.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHURQ_002020"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHURQ_002020"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR0823799.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHURQ_002021"
     CDS             3423..4061
                     /locus_tag="ACHURQ_002021"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0823800.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHURQ_002022"
     CDS             4066..4842
                     /locus_tag="ACHURQ_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR0823801.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHURQ_002023"
     CDS             4931..6295
                     /locus_tag="ACHURQ_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR0823802.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHURQ_002024"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHURQ_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR0823803.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHURQ_002025"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHURQ_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR0823804.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHURQ_002026"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHURQ_002026"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR0823805.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHURQ_002027"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHURQ_002027"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR0823806.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHURQ_002028"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHURQ_002028"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR0823807.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHURQ_002029"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHURQ_002029"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR0823808.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHURQ_002030"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHURQ_002030"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR0823809.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHURQ_002031"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHURQ_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR0823810.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHURQ_002032"
     CDS             complement(12844..13167)
                     /locus_tag="ACHURQ_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR0823811.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHURQ_002033"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHURQ_002033"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR0823812.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHURQ_002034"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHURQ_002034"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR0823813.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHURQ_002035"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHURQ_002035"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR0823814.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHURQ_002036"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHURQ_002036"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR0823815.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHURQ_002037"
     CDS             complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHURQ_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR0823816.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHURQ_002038"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHURQ_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHURQ_002039"
     CDS             complement(19048..>19530)
                     /gene="cas6e"
                     /locus_tag="ACHURQ_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0823817.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4605 a         5270 c         4896 g         4759 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gcccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaagcg
//
LOCUS       DAZOZY010000067        19198 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZOZY010000067 DAZOZY010000000
VERSION     DAZOZY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19198
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.067"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..72
                     /locus_tag="ACHURQ_002040"
     CDS             <1..72
                     /locus_tag="ACHURQ_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823818.1"
                     /translation="ARRRCDVLFAMMRDGTFYTPQGS"
     gene            481..930
                     /gene="alaE"
                     /locus_tag="ACHURQ_002041"
     CDS             481..930
                     /gene="alaE"
                     /locus_tag="ACHURQ_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR0823819.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(967..1311)
                     /locus_tag="ACHURQ_002042"
     CDS             complement(967..1311)
                     /locus_tag="ACHURQ_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR0823820.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1463..1792
                     /locus_tag="ACHURQ_002043"
     CDS             1463..1792
                     /locus_tag="ACHURQ_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR0823821.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2040..2285
                     /gene="nrdH"
                     /locus_tag="ACHURQ_002044"
     CDS             2040..2285
                     /gene="nrdH"
                     /locus_tag="ACHURQ_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR0823822.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2282..2692
                     /gene="nrdI"
                     /locus_tag="ACHURQ_002045"
                     /pseudo
     CDS             2282..2692
                     /gene="nrdI"
                     /locus_tag="ACHURQ_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2665..4809
                     /gene="nrdE"
                     /locus_tag="ACHURQ_002046"
     CDS             2665..4809
                     /gene="nrdE"
                     /locus_tag="ACHURQ_002046"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR0823823.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4819..5778
                     /gene="nrdF"
                     /locus_tag="ACHURQ_002047"
     CDS             4819..5778
                     /gene="nrdF"
                     /locus_tag="ACHURQ_002047"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR0823824.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6134..7336
                     /gene="proV"
                     /locus_tag="ACHURQ_002048"
     CDS             6134..7336
                     /gene="proV"
                     /locus_tag="ACHURQ_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR0823825.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7329..8393
                     /gene="proW"
                     /locus_tag="ACHURQ_002049"
     CDS             7329..8393
                     /gene="proW"
                     /locus_tag="ACHURQ_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR0823826.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8451..9443
                     /gene="proX"
                     /locus_tag="ACHURQ_002050"
     CDS             8451..9443
                     /gene="proX"
                     /locus_tag="ACHURQ_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR0823827.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9734..10918
                     /locus_tag="ACHURQ_002051"
     CDS             9734..10918
                     /locus_tag="ACHURQ_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0823828.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            11042..11779
                     /gene="ygaZ"
                     /locus_tag="ACHURQ_002052"
     CDS             11042..11779
                     /gene="ygaZ"
                     /locus_tag="ACHURQ_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR0823829.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11769..12104
                     /gene="ygaH"
                     /locus_tag="ACHURQ_002053"
     CDS             11769..12104
                     /gene="ygaH"
                     /locus_tag="ACHURQ_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR0823830.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12195..12725
                     /gene="emrR"
                     /locus_tag="ACHURQ_002054"
     CDS             12195..12725
                     /gene="emrR"
                     /locus_tag="ACHURQ_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR0823831.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12852..14024
                     /gene="emrA"
                     /locus_tag="ACHURQ_002055"
     CDS             12852..14024
                     /gene="emrA"
                     /locus_tag="ACHURQ_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR0823832.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            14041..15579
                     /gene="emrB"
                     /locus_tag="ACHURQ_002056"
     CDS             14041..15579
                     /gene="emrB"
                     /locus_tag="ACHURQ_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR0823833.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15643..16158)
                     /gene="luxS"
                     /locus_tag="ACHURQ_002057"
     CDS             complement(15643..16158)
                     /gene="luxS"
                     /locus_tag="ACHURQ_002057"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR0823834.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16308..17864)
                     /gene="gshA"
                     /locus_tag="ACHURQ_002058"
     CDS             complement(16308..17864)
                     /gene="gshA"
                     /locus_tag="ACHURQ_002058"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR0823835.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17937..18365)
                     /locus_tag="ACHURQ_002059"
     CDS             complement(17937..18365)
                     /locus_tag="ACHURQ_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR0823836.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18362..18928)
                     /gene="yqaB"
                     /locus_tag="ACHURQ_002060"
     CDS             complement(18362..18928)
                     /gene="yqaB"
                     /locus_tag="ACHURQ_002060"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR0823837.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(18958..>19198)
                     /locus_tag="ACHURQ_002061"
     CDS             complement(18958..>19198)
                     /locus_tag="ACHURQ_002061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823838.1"
                     /translation="TFSNEGIEEIAVLTAGTRKDNVAPATARRANVSESSSRMHHLLQ
                     KISFKNISFLNNSFIHFLNFLLLRHQRQNQAITLK"
BASE COUNT         4572 a         4966 c         4845 g         4815 t
ORIGIN      
        1 gcgagacgac gctgcgacgt tctgttcgcc atgatgcgcg acgggacttt ttataccccg
       61 caggggtcat aacatgcttg acaacttaat aggggcaccc ccctcactgg tgctttctgc
      121 tgttaatgta cattttctgg catcaaaaat gctgtaaatg acacggtcac gacatacacc
      181 gaattctcta cgcttatagc gttacctcac ccccaaactt tcattagtga aattaaaggc
      241 aaggtcatat ctaattgatt taattgaaaa taaatcatat cggcatgtaa aaatccacaa
      301 tgtacaaaaa acgagccgtt acggaatatt ttatctacaa aaactgacta aataaaaatt
      361 tttcactaat tgaatagtca tagccagcga tatacgctat gcgaaaatgc agatggcaat
      421 gagatccact gctttcatct ccattaacat cccattacgc ttttattaag gagcattagc
      481 atgttctcac cgcagtcacg cttgcgtcat gcagttgcag atacgttcgc gatggttgtt
      541 tactgttctg tcgtgaacat gtgtattgaa gttttcctct ccggaatgag cttcgaacag
      601 tctttttatt ccagattggt agcgattccg gtgaacatct taattgcatg gccatacggt
      661 atgtaccgcg atctatttat gcgcgcggca cgcaaagtta gcccgtcggg ctggataaaa
      721 aatctggctg atatcctggc ttatgtgacg ttccagtcac cggtgtatgt ggcgatcttg
      781 ttagtggtgg gcgcagactg gcatcagatt atggcggcgg tcagttcaaa catcgttgtt
      841 tcgatgttga tgggggcggt ttatggctac ttcctcgatt attgccgccg actgtttaaa
      901 gtcagccatt accagcaggt aaaagcctga ctcttctttt cgcgactggc atcgccagtc
      961 gcgtctttaa ctggcttcgc caaataaacc attcaaataa cgttcaagcg caacacgcga
     1021 actgaagcca tgcgggatac cgtaatgctc gtgtcgctcg cctgctaaat agagcggaaa
     1081 ttgttgataa tgatcgcaag ttttaatatc ggaagcgggt tcagccagtg tcgaactacg
     1141 ttcttttaat gttggatgaa ttaccagtgc ggtacgtccc attcgcgctt cgcgattaac
     1201 atagacataa ttttccccac ggcggtaacc ataagctttc tgggttacca catcgacagt
     1261 aaaaccgact ttttcaagta cgcgcgccac ctcgtctggt cgtaaataca tatttgatcc
     1321 tcgttattat caaccgcggg cctaccttac ctgattgcgc attcacaacg ctttcagaaa
     1381 agtccataaa gccgcgatga acctcagtta agaaaattat ggtctacact gaaaattaca
     1441 tcgaattcta atgggggatc atatgtttaa ccgaccgaac cgcaacgacg ttgatgatgg
     1501 cgtgcaggat atccagaatg atgtcaatca attagctgac agcctcgaat ctgtattgaa
     1561 gtcctggggc agcgacgcca aaggggaagc tgaagccgca cgcagtaaag ctcaggcatt
     1621 gctgaaagaa accagggcgc gaatgcatgg tcgtactcgc gtccagcaag ccgcacgcga
     1681 tgccgttggc tgcgcggatt cttttgttcg tgaaagaccc tggtgtagcg tgggtacagc
     1741 agctgcggta ggtattttta tcggcgcact gttaagcatg cgcaaatcgt agtgcaaaaa
     1801 tgataataaa tacgcgtctt taaccccgaa gcctgtcttc ggggtttctt tttgcctggt
     1861 gaatcacaaa aatccccctc ccccgtcacg ctcatatcta gggtaatttc gatcactatt
     1921 tgctatatat tgtgtggttg aatctttttt caactacatc tagtatctct gtatcaacag
     1981 agagacaacc cgacgcgtat catcgcgccg tatcttcatt ttaaacggaa atacgaatca
     2041 tgcgcataac tatttacact cgtaacgatt gcgttcagtg ccacgccacc aaacgggcga
     2101 tggaaaaccg gggctttgat tttgaaatga ttaatgtcga tcgcgttcct gaagcagcgg
     2161 aagcgttgcg tgctcagggc tttcgtcagt tgccggtagt gattgctggc gatcttagct
     2221 ggtctggttt ccgtccggac atgattaacc gtctgcatcc agcgccacac gcggccagtg
     2281 catgagccag ctcgtctact tctccagcag ctccgaaaac acgcagcgtt ttatcgaacg
     2341 tttaggtctg cccgcggtgc gcatcccgct caatgagcgg gaacggattc aggtagacga
     2401 gccttacatc ctgatcgtgc cctcttacgg cggcggcggt acggctggcg cggtgccacg
     2461 ataggtaatt cgctttttaa acgacgagca caaccgggcg ttgcttcgcg gcgttattgc
     2521 ttctggtaat cgcaactttg gtgaggcgta tggccgcgcc ggagatgtga ttgcccggaa
     2581 atgcggcgtg ccgtggctgt accgttttga actcatgggt acgcaaagcg atatcgaaaa
     2641 cgttcgtaaa ggagtaaccg aattttggca acgacaaccg cagaatgcct gacgcaggaa
     2701 acgatggatt accacgcgct gaatgcgatg cttaacctct acgatagcgc aggtcgcatt
     2761 cagttcgata aagaccgcca ggccgttgac gcctttatgg cgacgcatgt gcgtccgaac
     2821 agtgtgacct tcagtagcca gcagcagcgc ctgaactggc tggtcaacga aggttactat
     2881 gatgaaagcg ttcttaatcg ctactctcgc gactttgtca ttacgctgtt tgcccacgca
     2941 cacaccagcg gttttcgttt ccagacattc ctcggggcat ggaagtttta caccagctat
     3001 acgttgaaga cattcgacgg taaacgttat ctggaagatt ttgccgatcg agtcacgatg
     3061 gtggcgctga cgctggcaca aggcgatgcg acgctggcgt tgcaactgac cgatgaaatg
     3121 ctgtcaggac gctttcagcc agccacgccg acattcctca actgcggtaa gcagcagcgc
     3181 ggcgaactgg tttcctgttt tctgctgcgt attgaagaca atatggagtc gattggccgg
     3241 gcggtaaatt ccgcactgca actgtcgaaa cgcggcggcg gcgtggcatt tttgctgtcg
     3301 aatctgcgag aagcgggcgc gccaattaaa cgtattgaaa atcaatcttc tggcgtaatt
     3361 ccggtgatga aaatgctgga agacgcattt tcctatgcca accaactcgg cgcgcgtcag
     3421 ggggcaggtg cagtctattt acatgctcat catcccgata ttctgcgttt tctcgataca
     3481 aaacgggaaa atgccgacga aaaaatccgc attaaaacac tgtcgctggg cgtggtgatc
     3541 ccggatatca ctttccatct ggcaaaagag aatgcgcaga tggcgctgtt ttcgccctat
     3601 gacgttgagc gagtttatgg caagccgttt gccgatatcg ccatcagcga acactatgac
     3661 gaactggttg ccgatgaacg catccgcaaa aaatacctca acgcccgtga tttcttccag
     3721 cggctggcag aaatccagtt tgagtccggc tatccctaca tcatgtatga agacacggta
     3781 aaccgtgcta accctgtcgc cgggcgcata aatatgagta atctctgctc agagattttg
     3841 caggtcaact gtgcctcaga gtatgacgag aatctcgact atgcccgcac aggccatgat
     3901 atctcctgca atttaggttc gttgaatatt gcgcacacca tggattcccc cgattttgcc
     3961 cgcacggtag agactgccgt gcgcggttta acggcggtgt cagatatgag tcatatccgc
     4021 agcgtgccgt ccatcgaagc cggaaatgcc gcctcacacg ccatcggact ggggcagatg
     4081 aatttacacg gctatctggc gcgagaaggc atcgcttatg gttcgccgga agcactggat
     4141 ttcaccaatc tctatttcta tgccatcacc tggcacgcac tgcgtacctc gatgttgctg
     4201 gcgcgcgaac gcggtgaaac cttcgccggg ttcaaacagt cacgttatgc cagtggtgaa
     4261 tattttagcc aatatctgca aggtaactgg cagccgaaaa cggcgaaagt tggcgaactg
     4321 tttgcccgca gcggtattac gttacctacc cgtgagatgt gggcgcaact gcgcgacgac
     4381 gtgatgcgct acggcattta caaccagaat cttcaggcgg tgccgccaac cggttctatc
     4441 tcttatatca accatgctac gtcgagtatt catccgattg tggcgaaagt agagatacgc
     4501 aaagagggca aaacaggacg cgtttactac cctgccccgt ttatgactaa cgaaaatctg
     4561 gcgctgtatc aggatgctta cgaaatcggc gcagaaaaga tcatcgacac ctacgcggaa
     4621 gcgactcgcc atgtcgatca gggtttgtcg ctgacgctgt ttttccccga taccgccacc
     4681 actcgcgata tcaacaaagc gcagatttac gcctggcgca aaggtatcaa aacgctctat
     4741 tacattcgcc tgcgtcagat ggcgctggaa ggcactgaaa ttgaaggctg cgtctcctgt
     4801 gcactttaag gaatatctat gaaactctca cgtatcagcg ccatcaactg gaacaagata
     4861 tctgacgata aagatctgga ggtgtggaat cgcctgacca gcaatttctg gctaccagaa
     4921 aaggtgccgc tgtcgaacga tattcctgcc tggcagacat taactgtcgt agaacaacaa
     4981 ctgacgatgc gcgtttttac tggcctgacg ctgctcgaca cgctgcaaaa tgttatcggc
     5041 gcgccttcgc tgatgcccga tgcactcacg cctcatgaag aagcggtatt atcgaatatc
     5101 agctttatgg aagcggttca tgcccgctct tacagttcga ttttctcgac gctgtgccag
     5161 accaaagatg tcgatgccgc ctacgcctgg agtgaagaaa acgcaccgtt gcagcgaaaa
     5221 gctcagatta ttcagcaaca ttatcgcggt gatgatccgc tgaaaaagaa aatcgccagt
     5281 gtgtttcttg aatctttttt gttctattcc ggtttctggc tgccgatgta tttttccagc
     5341 cgcggaaagc tgaccaatac cgcagacctg atccgtctga ttatccgtga tgaagcagtc
     5401 cacggttact acataggcta taaatatcag aaaaacatgg aaaagatatc tctgggacaa
     5461 cgtgaagagt tgaagagttt cgccttcgat ttgttgctgg aactctacga caacgagctg
     5521 caatacaccg atgagctgta cgccgaaacc ccgtgggctg acgatgtgaa agcgtttctc
     5581 tgttacaacg ccaataaggc tttgatgaat ctgggctacg aacctttatt tcccgcagaa
     5641 atggcggaag tgaatccggc aatcctcgcc gcgctttcgc cgaatgccga tgaaaatcac
     5701 gatttctttt ccggttcagg ctcctcttat gtgatgggga aagcggttga aacagaagat
     5761 gaagactgga atttctgagg gtgttatttt caaaaatatc actacccgcg acagggaaat
     5821 aattcccgcc aaatagcttt ttatcacgca aatattttgt ggtgatctac actgatactc
     5881 tgttgcatta ttcgcctgaa accacaatat tcaggcgttt tttcgctatc tttgacaaaa
     5941 aatatcaact tttctcgatt tgctctcagc ccttatatca cgggaaattc cggcgatttg
     6001 ctcgcatcaa tattcatgcc acatttgcca tcaggggttg cctcagattc tcagtatgtt
     6061 agggtagaaa aaagtaacta tttccattgg gtaatatatc gacataaaca aataacagga
     6121 atctttctat tgcatggcaa ttaaattaga aattaaaaac ctttataaaa tatttggtga
     6181 gcatccacag cgagcattca aatatatcga acaaggactt tcaaaagaac aaattctgga
     6241 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     6301 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     6361 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     6421 cgatgccaaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     6481 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     6541 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     6601 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     6661 ccgcgcgtta gcgattaatc cggatatttt attaatggac gaagccttct cggcgctcga
     6721 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     6781 caccattgtc tttatttccc acgatctcga tgaagccatg cgtattggcg accgaattgc
     6841 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     6901 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tattcagtgc
     6961 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaacccctg gcttcggccc
     7021 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     7081 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     7141 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     7201 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     7261 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     7321 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     7381 cgcaatccgc agacgcctgg ggtacaccga cgactgcacc gactgacggc ggtggtgctg
     7441 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     7501 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     7561 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacagtttcc
     7621 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     7681 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg
     7741 caatcggtgc ctggtcgcag gcaatggtga ctctggcgct ggtgttaacc gccctgctgt
     7801 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     7861 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtac
     7921 caatcgtcat gctgtttggt atcggtaacg tgccgggcgt agtggtgacg atcatctttg
     7981 ctctgccgcc gattatccgt ctgacgattc tggggattaa ccaggttccg gcggatctga
     8041 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     8101 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gctctttcta
     8161 tggtggtcat cgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     8221 tcggtcgtct ggatatgggc cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     8281 ttatcctcga ccgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     8341 gctggtacac cactggccct gtcggtctgc tgacccgccc attcattaag taactctgca
     8401 cttgcccggt aacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     8461 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gcagccgatc
     8521 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     8581 agacgctgct ggtcagtcgt gcactggaga aattaggtta taccgtcaat aaacccagcg
     8641 aagtggatta caacgttggc tacacctcac ttgcttccgg cgatgcaacc ttcaccgccg
     8701 tgaactggac gccgctgcat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     8761 attgtgaagg ggtattcgtt aacggcgcgg cacagggtta cctgatcgat aagaaaaccg
     8821 ccgaccagta caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     8881 tcgataccaa cggcgacggt aaagcggatt taaccggctg taacccaggc tggggctgcg
     8941 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     9001 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     9061 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     9121 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaacgcc gataccaaac
     9181 tgccgaatgg tgcgaattat ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     9241 cctgggccga gaaaaacccg gcagcagcga aactttttgc cattatgcag ttgccagtgg
     9301 cagatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     9361 agggccatgt tgatggttgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     9421 aggcgctggc agcgcagaag taatttttat tcgggcggat aagtcgtttt tgcctcatcc
     9481 gccattctgt gcacaatgcc tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaatac
     9541 actcattccg tatggcggat aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc
     9601 tgatgcgacg ctggcgcgtc ttatcatgcc tacaaaccac atcgcacatt tcaacaagct
     9661 atctttcgcc cccattcatc aacatccgct attattgatt tccagcttaa tcatcacctg
     9721 atgaacaaaa ataatgacta aacctaatca tgagcttagc ccggcgctga tcgtgctgat
     9781 gtctatcgcc accggtcagg cggtagccag taactattac gcccagccat tgctcgacac
     9841 catcgcgcgt aacttttccc tttccgccag ttcggcaggc tttattgtta ccgccgcgca
     9901 gttgggctat gcagcaggtc tgctgtttct tgttcccctc ggtgatatgt ttgaacgccg
     9961 ccgcctgatt gtctcgatga ccttactggc ggcaggcggc atgttaatca ctgccagcag
    10021 tcagtcgctg gcgatgatga tcctcggtac ggcattaacc ggtttattct cagtcgtggc
    10081 acaaattctg gttccgctgg cagcgacgct ggcttcaccg gataaacgcg gcaaagtggt
    10141 tggcaccatt atgagcggtc tgctgttggg gatcttgctg gcacgaaccg ttgccgggtt
    10201 actggcaaac ctcggcggct ggcgcaccgt cttttgggtt gcctcggtgt taatggcact
    10261 gatggcgcta gcattatggc gtggtctgcc acaaatgaaa tcagaaaccc acctcaacta
    10321 cccacagttg ttaggttccg ttttcagcat gtttatcagc gataagatcc tccgcacccg
    10381 cgcattgctg ggctgcctga cctttgccaa cttcagcatt ctctggacct caatggcctt
    10441 tttgcttgcc gctccacctt ttaactacag cgatggtgtc attggtctgt ttggacttgc
    10501 gggagctgcc ggagcgttgg gcgctcgtcc ggcgggcggt tttgccgata agggtaaatc
    10561 gcaccacacc acaactttcg gtctgctgct gctattactt tcatggctgg cgatctggtt
    10621 tggtcacact tccgtactgg cgctgattat cggcatcctg gtgctggatc tcaccgtaca
    10681 gggcgtgcat atcactaacc agacggtaat ttatcgaata caccctgatg cgcgtaatcg
    10741 cctgaccgca ggttacatga ccagctactt tattggcggt gccgccggtt cgctaatttc
    10801 agcctcagcc tggcaacatg gcggttgggc tggcgtttgt ctggctggcg cgacgattgc
    10861 cctagttaac ttactggtct ggtggcgagg ttttcatcgt caggaagccg caaattaagc
    10921 aatcagcaac cgttttcgtg gctttacact tataagggtg ttaagaagcc catcagtctg
    10981 ataaggttaa gatattcatt cagtctattt ataatattaa caatcgttaa gcgtacactc
    11041 tatggaaagc cctactccac agcctgctcc tggttcggcg accttcatgg aaggatgcaa
    11101 agacagttta ccgattgtta ttagttatat tccggtggcc tttgcgttcg gtctgaatgc
    11161 gacccgtctg ggattctctc ctctcgaaag cgtttttttc tcctgcatca tttatgcagg
    11221 cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg agtagtttgt gggttgctgc
    11281 actgaccgtc atggcaatgg atgttcgcca tgtgttgtat ggcccgtcac tgcgtagccg
    11341 tattattcag cgtctgcaaa aatcgaaaac cgccctgtgg gcgtttggcc tgacggatga
    11401 ggtttttgcc gccgcaacag caaaactggt acgcaataat cgccgctgga gcgagaactg
    11461 gatgatcggc attgccttca gttcatggtc atcgtgggta tttggtacgg taataggggc
    11521 attctccggc agcggcttgc tgcaaggtta tcccgccgtt gaagcagcat taggttttat
    11581 gcttccggca ctctttatga gtttcctgct cgcctctttc cagcgcaaac aatctctttg
    11641 cgttaccgca gcgttagttg gtgcccttgc aggcgtaacg ctattttcta ttcccgtcgc
    11701 cattctggca ggcattgtct gtggctgcct cactgcgtta atccaggcat tctggcaagg
    11761 agcgcccgat gagctatgag gttctgctgc ttgggttact ggttggcgcg gcgaattatt
    11821 gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc ccgcccaacc aaacgtggcg
    11881 cggtaggtat tttgctcgac accattggca tcgcctcgat atgcgctctg ctggttgtct
    11941 ctaccgcacc agaagtgatg cacgatacac gccgtttcgt gcccacgctg gtcggcttcg
    12001 cggtactggg tgccagtttc tataaaacac gcagcattat catcccaaca ctgcttagtg
    12061 cgctggccta tgggctcgcc tggaaagtga tggcgattat ataacccaca agaatcattt
    12121 ttctaaaaca atacatttac tttatttgtc actgtcgtta ctatatcggc tgaaattaat
    12181 gaggtcatac ccaaatggat agttcgttta cgcccattga acaaatgcta aaatttcgcg
    12241 ccagccgcca cgaagatttt ccttatcagg agatccttct gactcgtctt tgcatgcaca
    12301 tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa ggctcagggg attaacgaga
    12361 cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa ccacagtatt cagccttctg
    12421 aattaagttg tgctcttgga tcatcccgta ccaacgcgac gcgtattgcc gatgaactgg
    12481 aaaaacgcgg ttggatcgaa cgtcgtgaaa gcgataacga tcgccgctgc ctgcatctgc
    12541 aattaacgga aaaaggtcac gagtttttgc gcgaggtttt accaccgcag cataactgcc
    12601 tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga tcagctcgag caaatcaccc
    12661 gcaaattgct ctcccgtctc gaccagatgg aacaagacgg tgtggttctc gaagcgatga
    12721 gctaacgcgt catctcgctc aaaaatccag atttataaaa gaaaaaatga ccggccagca
    12781 tcgcaacatg ctggcctttt tggcaagcag gtcggctcag ccgatgagtt aagaagatcg
    12841 tggagaacaa tatgagcgca aatgcggaga ctcaaacccc gcagcaaccg gtaaagaaga
    12901 gcggcaaacg taagcgtctg ctcctccttc tcaccttgct ctttataatt attgccgtag
    12961 cgatagggat ttattgtttt ttggtactgc gtcatttcga agaaaccgat gacgcatacg
    13021 tggcagggaa tcagattcaa attatgtctc aggtgtctgg cagcgtgacg aaagtctggg
    13081 ccgataacac cgattttgta aaagaaggcg acgtgctggt cactctcgac ccgacagatg
    13141 ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc cagcgttcgc caaacccacc
    13201 agctgatgat taacagcaaa cagttgcagg cgaatattga ggtgcagaaa atcgccctcg
    13261 caaaagcaca aagcgactac aaccgccgtg tgccgctggg caatgccaac ctgattggtc
    13321 gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc ccaggcgcaa ctggacgtcg
    13381 ctattcaaca atacaatgcc aatcaggcga tgattctggg gactaaactg gaagatcagc
    13441 ctgccgtgca acaggctgcc accgaagtac gtaacgcctg gctggcgctg gagcgtaccc
    13501 gtattgtcag tccgatgacc ggttatgtct cccgccgcgc ggtacagcct ggggcgcaaa
    13561 ttagcccaac gacgccgctg atggcggtcg ttccagccac caatatgtgg gtggatgcca
    13621 actttaaaga gacgcagatt gccaatatgc gtatcggtca gccggtcact atcaccacgg
    13681 atatttacgg cgatgatgtg aaatacaccg gtaaagtggt tggtctggat atgggcacag
    13741 gtagcgcatt ctcactgctt ccggcgcaaa atgcgaccgg taactggatc aaagtcgttc
    13801 agcgtctacc tgtgcgtatc gaactggacc agaaacagct ggagcaatac ccgctgcgca
    13861 ttggtttgtc cacactggtg agcgtcaata ccactaaccg tgacggtcag gtactggcaa
    13921 ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg tgaaatcagc ctggcacctg
    13981 tcaataaact gatcgacgat atcgtaaaag ctaacgctgg ctaatccaga ggtgcgtgtg
    14041 atgcaacagc aaaaaccgct ggaaggcgcg caactggtca ttatgacgat tgcgctgtca
    14101 ctggcgacat tcatgcaggt gctggactcc accattgcta acgtggcgat ccccactatc
    14161 gccgggaatc tgggctcatc gctcagccag ggaacgtggg taatcacttc tttcggggtg
    14221 gcgaatgcca tctcgatccc gcttaccggc tggctggcaa agcgcgtcgg ggaagtgaaa
    14281 ctgttccttt ggtccaccat cgcctttgct attgcgtcgt gggcgtgtgg tgtctccagc
    14341 agcctgaata tgctgatctt cttccgcgtg attcagggga ttgtcgccgg gccgttgatc
    14401 ccgctttcgc aaagtctatt gctgaataac tacccgccag ccaaacgctc gatcgcgctg
    14461 gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg gcccgatcct cggcggttat
    14521 atcagcgata attaccactg gggctggata ttcttcatca acgtgccgat tggcgtggcg
    14581 gtggtgttga tgacactgca aactctgcgc ggacgtgaaa cccgcaccga acggcggcgg
    14641 attgatgccg tggggctggc actgctggtt attggtatcg gcagcctgca gattatgctc
    14701 gaccgcggta aagagctgga ctggttttca tcacaggaaa ttatcatcct taccgtggtg
    14761 gcggtggtgg ctatctgctt cctgattgtc tgggagctga ccgacgataa cccgatagtc
    14821 gatctgtcgt tgtttaagtc gcgcaacttc accatcggct gcttgtgtat cagcctcgcg
    14881 tatatgctct acttcggcgc tattgttctg ctgccgcagt tgttgcagga ggtctacggt
    14941 tacacggcga cctgggcagg tttggcctct gcgccggtag ggattattcc ggtgatcctg
    15001 tcgccgatta tcggccgctt cgcgcataaa ctggatatgc ggcggctggt aaccttcagc
    15061 tttattatgt atgccgtctg cttctactgg cgtgcctata cctttgaacc aggtatggat
    15121 tttggcgcgt cggcctggcc gcagtttatc caggggtttg cggtggcctg cttctttatg
    15181 ccgctgacca ccattacgct gtctggtttg ccaccggaac gactggcggc ggcatcgagc
    15241 ctctctaact ttacgcgaac gctggcgggg tctatcggca cgtcgataac cacgaccatg
    15301 tggaccaacc gcgagtcgat gcaccatgcg cagttgactg agtcggtaaa cccgttcaac
    15361 ccgaatgccc aggcgatgta cagtcaactg gaagggcttg ggatgacgca acagcaggca
    15421 tcaggctgga ttgcccagca gatcaccaat caggggctga ttatttccgc caatgagatc
    15481 ttctggatgt cagccgggat attcctcgta ctgctggggc tggtgtggtt tgctaaaccg
    15541 ccatttggcg caggtggcgg tggcggcggt gcgcactaag tacaactaag ccagttcatt
    15601 tgaactggct tttttcaatt aattgtgaag atagtttact gactagatgt gcagttcctg
    15661 caacttctct ttcggcagtg ccagttcttc gttgctgttg atgcgtacgt cacgttccag
    15721 aatgctacgc gcaatatcct gcgcttcctg caacgagtgc atctggtaag tgccacactg
    15781 gtagacgttc agttccggga tctgattctg atcctgcact ttcagcacgt cttccattgc
    15841 cgctttccag gcatcagcaa cacgctgctc atctggcgta ccaatcagac tcatataaaa
    15901 accggtgcgg cagcccattg gcgagatatc gataatctct acaccattac cgttaagatg
    15961 gttacgcata aaaccagcaa acaggtgctc cagggtatgg atccctcttt ctggcatcac
    16021 ttctttgttc ggcacgcaga agcgcagatc gaacacggtg attgcgtcgc catgcggggt
    16081 gttcattgtt ttcgccaccc gaactgcagg cgcttccatc cgggtatgat cgactgtgaa
    16141 gctatctaac aacggcattt agccacctcc ggtaattttt ttaaaaattt tctgaactct
    16201 ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt tgttatcatc atccctgaat
    16261 tcagagatga aattttggcc actcacgagt ggcctttttc ttttctgtca ggtgtgtttt
    16321 tccagccaca ccgcaaacgg ttcggtatca gcggtttcca tttcctgctg acggcgttca
    16381 gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa tttccagcgg ctcttcacgc
    16441 agcagattac ggtaggcttc tgcaaacgct ttgcctgttc cgccaatacc agtatcaatc
    16501 atagacctta agatacgggc agagaaagtc agatcgggat tatcgaagca ggcaaccagt
    16561 tcatcacaca ctttctgata cgcgtcgccg ccgttaatgc tatccagcgt ttgcgcgacg
    16621 cgtttcagat cgcggaacag atctttaccc acctgcagta acgggaactg cgcggtttcg
    16681 cagccgatac ccagcgtcag gcccggtttg cgaccttcaa ggatcacccg gttccagtta
    16741 acgcgcgtac aggcaagttc gctactgctc atttccggtg catcagccag cgcacaccag
    16801 accataaaca ggtcgaggaa tcgcacctgc tgttcatcta caccaatcgg cgagaacggg
    16861 ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc cacgtaacag cgcatcagaa
    16921 ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg gcgcatacag ttcgttttca
    16981 atctgcagca cgttgctatt gatttgcagc ctcttaccgt ctttctcaat accaatcttc
    17041 gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc ccgctacgta ctcgtaaaga
    17101 tcgttgaagg taataccaag attgctttgc gatttattgg tatagccgag atcgctcaaa
    17161 cgaagagagg tcgcatacgg caggtaatac ataccgcact cggttttctc aaacggcagc
    17221 gacgttggtt ttccttgcag gaaagaagaa caaatcgccg gagatgcacc aaacagataa
    17281 ggaatgaccc aaccgaaacg atagtaattg cggataacgc ggaaatagcc cgcagaaatt
    17341 ttctctttgg catcagcgcc cgagatatca ccgcacttcg cttgccagaa tgccattggc
    17401 aaagagaaat tgtagtgcac gccggaaatg gtttgcatca gcgcgccgta gcgatttttc
    17461 agcccttcac gatacagcgt tttaaagcgt ccggtgttag aagtgccgta ctgtgccagt
    17521 tcgatgtcct gaccttctgc gatgtagcat ggcatactta acggccacat ccgctcatcg
    17581 cccatattgc gcgccgtata acgatgcaga tcgcgcataa aggtcagcat atgttcaata
    17641 tcaccatcca ctggtgtaat gaattccagc aatgcttccg caaaatcggt agtaatccat
    17701 ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg ttgttgccag tgtgccatca
    17761 gcattaacac gcaaagtttc gcgctccagc ccacgctgta tcccctttaa cgcctgagga
    17821 tgtttttcca gccaggccag cgcctgtgat acgtccggga tcaaattgac ctcccgcctg
    17881 tcaaaatcgt tttaattagc ataactgtaa tggtgaccat atgtgcaggc ctacaattag
    17941 tgccaccaca tcatgccctg aacggtcgct gctgcaactg caacatagcg taacgcttta
    18001 ccaaggcaca aaaaaaagat taccggtccc cacgagatgc gcatccatcc cgctaacaga
    18061 cacagtaaat cgccaaccac gggcatccag cttaataata gcgtgactgc accatagcgt
    18121 ttcagccagc cgatagcttt ctcttgccag cgcgatgttt tacgcaatgg aaagaaacgc
    18181 ccaaggataa cgttagttaa ccctccaagg ctattaccca ttgttgctgt taaaactaaa
    18241 acccagggat gactgatccc ggaaagcaac attgccacca gcacgacttc ggagttgccg
    18301 ggtaatagtg tagcgctgag aaaactactg gcaaacaacg agaaaagcga taacgcttca
    18361 ctcacagcaa gcgaacgtcc acagcgtcca tacctgcggc acgggccgcc tgaataccga
    18421 aatcggcatc ttcaaagacc acacactgcg tcggttgcac gcccatacgc tgcgcgcaca
    18481 acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg atcggcagcg acgacggcgt
    18541 caaaataatg gcgtaatccc aggtgcgcca gcaatgcctc agcgatggcg ctttcactcc
    18601 ccgttcctac agccattggg cgacgaccat gccaactttt caccacatca acaagaggaa
    18661 gcggttcgac gctatccagc agcatacttc ttactgcttc tgttttttca cgcgctaacg
    18721 catgcgggtc gagatcggcc tgattcagct caataattgc ctgagcaata cgccaggtgg
    18781 gcgatccatt aagcgcaatc atcgcctgaa tatcgtactg aagaccgtag tgccctaata
    18841 cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag gattgtgcca tccatatcaa
    18901 aaattaaacc tgcataacgc tcgtacatgg tcttctcgca agtcgaaaaa tatgacgcta
    18961 ctttagcgta attgcttgat tttgtcgctg atggcgaagg agaagaaaat tcaggaaatg
    19021 gataaaggag ttatttagaa atgagatatt tttgaaggaa attttttgga gaagatggtg
    19081 catccgggag gatgactcac tgacgttcgc ccttcgggcc gttgctggcg caacgttatc
    19141 cttcctggtg cctgcggtta ataccgctat ctcttcgata ccttcattgc tgaaggtt
//
LOCUS       DAZOZY010000068        18847 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZOZY010000068 DAZOZY010000000
VERSION     DAZOZY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.068"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(104..1453)
                     /gene="lysC"
                     /locus_tag="ACHURQ_002062"
     CDS             complement(104..1453)
                     /gene="lysC"
                     /locus_tag="ACHURQ_002062"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR0823839.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1545..1741)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            1978..3627
                     /gene="pgi"
                     /locus_tag="ACHURQ_002063"
     CDS             1978..3627
                     /gene="pgi"
                     /locus_tag="ACHURQ_002063"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR0823840.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4089..4331
                     /gene="yjbE"
                     /locus_tag="ACHURQ_002064"
     CDS             4089..4331
                     /gene="yjbE"
                     /locus_tag="ACHURQ_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR0823841.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            4445..5083
                     /locus_tag="ACHURQ_002065"
     CDS             4445..5083
                     /locus_tag="ACHURQ_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR0823842.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5080..5817
                     /locus_tag="ACHURQ_002066"
     CDS             5080..5817
                     /locus_tag="ACHURQ_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0823843.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            5817..7913
                     /locus_tag="ACHURQ_002067"
     CDS             5817..7913
                     /locus_tag="ACHURQ_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0823844.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(7960..8238)
                     /locus_tag="ACHURQ_002068"
     CDS             complement(7960..8238)
                     /locus_tag="ACHURQ_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823845.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            8239..8382
                     /locus_tag="ACHURQ_002069"
     CDS             8239..8382
                     /locus_tag="ACHURQ_002069"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823846.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            8508..8918
                     /gene="psiE"
                     /locus_tag="ACHURQ_002070"
     CDS             8508..8918
                     /gene="psiE"
                     /locus_tag="ACHURQ_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR0823847.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(8962..10437)
                     /gene="xylE"
                     /locus_tag="ACHURQ_002071"
     CDS             complement(8962..10437)
                     /gene="xylE"
                     /locus_tag="ACHURQ_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR0823848.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10809..11699)
                     /gene="malG"
                     /locus_tag="ACHURQ_002072"
     CDS             complement(10809..11699)
                     /gene="malG"
                     /locus_tag="ACHURQ_002072"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR0823849.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(11714..13258)
                     /gene="malF"
                     /locus_tag="ACHURQ_002073"
     CDS             complement(11714..13258)
                     /gene="malF"
                     /locus_tag="ACHURQ_002073"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFR0823850.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(13412..14602)
                     /gene="malE"
                     /locus_tag="ACHURQ_002074"
     CDS             complement(13412..14602)
                     /gene="malE"
                     /locus_tag="ACHURQ_002074"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR0823851.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            14967..16082
                     /gene="malK"
                     /locus_tag="ACHURQ_002075"
     CDS             14967..16082
                     /gene="malK"
                     /locus_tag="ACHURQ_002075"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR0823852.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            16154..17494
                     /gene="lamB"
                     /locus_tag="ACHURQ_002076"
     CDS             16154..17494
                     /gene="lamB"
                     /locus_tag="ACHURQ_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR0823853.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            17646..18566
                     /gene="malM"
                     /locus_tag="ACHURQ_002077"
     CDS             17646..18566
                     /gene="malM"
                     /locus_tag="ACHURQ_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR0823854.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         4710 a         4854 c         4898 g         4385 t
ORIGIN      
        1 tcgataaaga cgttggttaa cagagtacag gctcgtttat tgataagcat agtgacaaga
       61 aaatcaatac ggcccgaaat atagcttcca ggccatacag tatttactca aacaaattac
      121 tatgcagttt ttgcaccacc tgctcggcat cttcgccggg caccaggaag cacaggttat
      181 ggctggatgc gccgtaacaa atcatgcgaa tgttgaacgg ttccagtacg ccgaatacct
      241 ctttgccaac gccgcaggct tttgacaggt cattgccaat caacgcgacc agcgccagac
      301 cttcttctac ttccacccgg cacagtgcgg aaagctccat cagcagagat tgcgtcagca
      361 acgtatcgcc agtggaggtt gaaccggtgg tatcaagggt taatgccacg ctcacttctg
      421 acgtggtgat taagtctacc gaaatattat gccgcgcgag gatgccgaaa acttccgcga
      481 ggaaaccgcg agaatgcagc atattcaggc tgtgcaaagt gagcagagtc tgattgcgac
      541 gaagcgccag cgcgcggaac agcggcggat tttcagtttt attgcacacc agcgtaccac
      601 ctgcgcgtgg gtctttgctg gagccgacaa agaccgggat atcgctgcgt actgcgggta
      661 gcaacgttgc cggatgcagt acttttgcac caaaagttgc catctctgcc gcttcggcaa
      721 acgcgatttc atcaatgcgt tttgctgcgg aaactacgcg tggatcggtg gtgtagatgc
      781 ccgggacgtc ggtccagata tcaacacgag atgcgtgtaa agcctccgcc agcaaggctg
      841 ccgtataatc gctgcctcca cggccaagcg tcgttgtacg acctttattt tcgctaccga
      901 taaatccctg ggtgatcact aagccttcat tgaggcgtgg gagcagctgc agcgcggcca
      961 gttccgccag cgcggctaca tctggctctg cacgaccaaa tcggtcgttg gtacgcatca
     1021 ctttacgtac atcaaaccac tgtgcctgaa catcgcgttc gcgcaggatt tcgacaaaca
     1081 gcagggtcga catcagctcg ccgtggctga ccagctcatc tgtcagcgcc ggagacgttg
     1141 ccagcgccgc cgcttctgcc agaacagtaa tgttctccag cagacgttca atctcttcac
     1201 ggataacgtt cgggtaacgc agacgttcca gaatggcaaa ctggatgttg cgaatagcgt
     1261 cgagtttttc gaatcgctcg ccaggttcca gcccttccgc taaagcgacc agcagattag
     1321 tgataccagc agaagccgag aggactacta aacgcacgtt ggcatcagaa agcacaatat
     1381 cagcgctgcg gttcatggcg tcaaaatcag ctacgctggt accgccaaat ttggagacaa
     1441 caatttcaga cataactacc tcgtgtcagg ggatccattt tcagccttgg cacaagggaa
     1501 gagcggaaga cgggtgggcg cagagcgata cttcgctact attttcaccc agaagtgctc
     1561 caccacttgc gaaacgcccg actgcgaacg cttctggtga caacccaggg gattcagccc
     1621 ctgtagccga tgatgaacgt ggccagccgt tcaatcacct cggcgatgca ccccctcagg
     1681 tgttatcaca ggactggctc ctccaacacc gttacttggg caacgcgcct cttctggcct
     1741 gcgctagcgc aggtagtaca tttataaata aagggtgagc ggggcggttg tcaacgacgg
     1801 ggtcatgcgg atttttcatc cactcctggc ggtcagtagt tcagctaata aatgcttcac
     1861 tgcgctaagg gtttacactc aacattacgc taacggcact aaaaccatca catttttctg
     1921 tgactggcgc tacaatcttc caaagtcaca attctcaaaa tcagaagagt attgctaatg
     1981 aaaaacatca atccaacgca gaccgctgcc tggcaggcac tacagaaaca cttcgatgaa
     2041 atgaaagacg ttacgatcgc cgatcttttt gctaaagacg gcgatcgttt ttctaagttc
     2101 tccgcaacat tcgacgatca gatgctggtg gattactcca aaaaccgcat cactgaagag
     2161 acgctggcga aattacagga tctggcgaaa gagtgcgatc tggcgggcgc gattaagtcg
     2221 atgttctctg gcgagaagat caaccgcact gaaaaccgcg ccgtgctgca cgtagcgctg
     2281 cgtaaccgta gcaatacccc gattctggtt gatggcaaag acgtaatgcc ggaagtcaac
     2341 gcggtgctgg agaagatgaa aaccttctcg gaagcgatta tctccggtga gtggaagggt
     2401 tataccggca aagcaatcac tgacgtagtg aacatcggga tcggtggttc tgacctcggc
     2461 ccgtacatgg tgaccgaagc actgcgtccg tacaaaaatc acctgaacat gcactttgtt
     2521 tctaacgtcg atgggactca catcgctgaa gtgctgaaaa aagtaaaccc ggaaaccacg
     2581 ctgttcctgg tagcatctaa aaccttcacc actcaggaaa ctatgaccaa cgcccatagc
     2641 gcgcgtgact ggttcctgaa agcggcaggt gatgaaaaac acgttgcaaa acactttgcg
     2701 gcgctttcca ccaatgccaa agccgttggc gagtttggta ttgatactgc caacatgttc
     2761 gagttctggg actgggtcgg cggccgttac tctttgtggt cagcgattgg cctgtcgatt
     2821 gttctctcca tcggctttga taacttcgtt gaactgcttt ctggcgcaca cgcgatggac
     2881 aagcatttct ccaccacgcc tgccgagaaa aacctgcctg tcctgctggc gctgattggc
     2941 atctggtaca acaatttctt tggtgcggaa actgaagcga ttctgccgta tgaccagtat
     3001 atgcaccgtt tcgcggcgta cttccagcag ggcaatatgg agtccaacgg taagtatgtt
     3061 gaccgtaacg gtaacgttgt ggattaccag actggcccga ttatctgggg tgaaccaggc
     3121 actaacggtc agcacgcgtt ctaccagctg atccaccagg gaaccaaaat ggtaccgtgc
     3181 gatttcatcg ccccggctat cacccataac ccgctctctg atcaccacca gaaactgctg
     3241 tctaacttct tcgcccagac cgaagcgctg gcgtttggta aatcccgcga agtggttgag
     3301 caggaatatc gcgatcaggg taaagatccg gcaacgcttg actacgtggt gccgttcaaa
     3361 gtgttcgaag gtaaccgccc gaccaactcc atcctgctgc gcgaaatcac tccgttcagc
     3421 ctgggtgcgt tgattgcgct gtatgagcac aaaatcttta ctcagggcgt gatcctgaac
     3481 atctttacct tcgaccagtg gggcgtggag ttgggtaaac aactggcgaa ccgtattctg
     3541 ccagagctga aagatgacaa agaaatcagc agccacgata gctcgaccaa tggtctgatt
     3601 aaccgctata aagcgtggcg cggttaatca tcatgcgtgc ctgatgccga tatgtaggcc
     3661 ggataaggcg tttacgccgc atccggcaac cggagcctga tgccaatatc gacattaatg
     3721 catagtatga ggagttaaat aaccttaaaa acaatgccga caataagtcg gcattgtttt
     3781 atcagataaa tccccttgtc tgtgatttaa cggaaatcat accgtgaggt taatcctaaa
     3841 atagattttt aatcgttgtt tatttcggag aatacgcaga ttaattactt ttgtttttat
     3901 tttaagttta tgatttttat tgttatttaa atataagttg aaacttatat ttgatattca
     3961 ttccaattat cctaaaacac catcgctaat tccccgcgcc gtaattcgca tgctttagtt
     4021 gtgtatactc gatcccgccc gaaatgtttt tgggtaaatc tccattcatt caatgaaggg
     4081 aaattgttat gaaaaaagtt ctgtatggca tttttgccat atctgcgctt gcggcgactt
     4141 ctgtgtgggc tgcacctgta caggtgggcg aagcggcagg ggcggcagca acgtcggttt
     4201 cggcggggag ttcctccgcg accagcgtca gcaccgtaag ctcggcggtg ggtgtcgcgc
     4261 tcgcggcaac cggtggcggt gatggttcta ataccgggac cacaacaacc acaaccacca
     4321 gtacccagta ataaagtatg tatccccaaa ataattcgag tcattgcatc tgtggcttga
     4381 aatatgaagg gattaaccat aaccacactc cggtgtggtt attctgcccc tctggagaag
     4441 agtcgtgaag cgacctgcac tcattcttat ctgcctgtta ttacaggcct gttcagccac
     4501 gactaaagag ctgggcaatt cactgtggga cagtctgttc ggcacgccag gcgtacagct
     4561 gacggacgat gatattcaaa atatgcccta cgccagccag tacatgcagc ttaatggcgg
     4621 gccgcaattg tttgtggtgc tggccttcgc tgaagacgga caacaaaaat gggtcactca
     4681 ggatcaagcc actcttgtca cacaacatgg tcgtctggtg aagactttgc ttggcggcga
     4741 caacctgatt gaagtgaata accttgccgc cgacccgctg attaaacccg cacaaattgt
     4801 tgatggtgca acatggacgc gcacgatggg ctggaccgag taccagcagg tacgctacgc
     4861 caccgcacgc tcagtcttca aatgggatgg caccgatacc gtcaaggtcg gcagcgatga
     4921 aaccctggtt cgcgtgctgg acgaagaagt ctccaccgac caggcgcgct ggcataaccg
     4981 ctactggatc gacagcgaag ggcaaattcg ccagtcggaa cagtatctcg gcgcggatta
     5041 tttcccggtg aaaaccactc tcatcaaggc ggcaaaacaa tgattaaaca aactattgtc
     5101 gcgttgcttt tgagtgtggg agcgtcatcg gtcttcgcgg caggaaccgt caaggtgttc
     5161 agcaatggca gcagtgaggc caaaacgcta acgggcgcag agcatttaat cgatctggta
     5221 gggcaacctc gactggcaaa cagctggtgg cctggtgcgg tgattagcga agagctggca
     5281 acggcggcag cattgcgtca gcagcaggcg ttgctgacac ggctggcaga acagggcgca
     5341 gattccagca ccgacgatgc cgccgccatt aacgccttac gccagcaaat ccaggcattg
     5401 gaagtgacag gcaggcaaaa aatcaatctt gatcccgata tcgtccgcgt tgccgaacgc
     5461 ggtaacccgc cgttgcaggg caactacacg ctgtgggtcg gaccaccgcc gtccacggtc
     5521 acgttgttcg ggcttatcag ccatcctggc aatcagccat tcactccagg tcgcgacgtg
     5581 gcgagctatc tctctgacca gagcctgctc agcggtgcgg atcgcagcta cgcgtgggtc
     5641 gtttacccgg acggacgcac gcaaaaagcg ccggtggctt actggaacaa gcgtcacgta
     5701 gagccgatgc ccggcagcat tatttatgtt ggcctcgcag actccgtctg gagtgagacg
     5761 cctgatgccc ttaacgccga cattcttcag actctgacgc agcggatacc ccaataatga
     5821 aaaaaagaca tctgcttagc ttactggcgc tgggcattag cacagcttgc tacggcgaaa
     5881 catatcctgc gcccattggt ccgtcgcagt cggatttcgg tggcgtagga ttattacaaa
     5941 ctcccaccgc gcgtatggca cgggaagggg agttgagtct gaactatcgc gataacgatc
     6001 agtaccgtta ttactcagct tcagtgcaac tcttcccgtg gctggaaaca acgctgcgtt
     6061 acaccgacgt gcgcacccgg cagtacagca gcgtcgaagc gttctctggc gatcaaacgt
     6121 ataaagataa agccttcgat ctcaaactgc gtttgtggga agagagttac tggctgccgc
     6181 aagtggcggt tggtgcgcgg gatatcggcg gtacggggct gtttgatgcg gaatatcttg
     6241 ttgccagcaa agcctggggg ccgttcgatt ttacgctcgg cctgggctgg ggatatctgg
     6301 gcaccagcgg taatgtgaaa aatccgctct gttcagccag tgataaatat tgctatcgcg
     6361 ataacagcta caaacaagcg ggatctatcg acggcagtca gatgttccac ggcccggcct
     6421 cactgtttgg cggcgtggaa taccagacgc cctggcaacc gctgcgcctg aaactggagt
     6481 atgaaggtaa taattatcag caggattttg ccgggaagct ggagcaaaaa agtaagttta
     6541 acgtcggggc gatttatcgc gttaccgatt gggccgacgt taaccttagc tatgaacgtg
     6601 gcaacacctt tatgtttggc gtcacgctgc gcaccaactt taacgatctg cgcccgtcct
     6661 acatcgataa cgcccgcccg caatatcaac cgcagccgca ggatgccatt ttgcagcact
     6721 cggtggtggc gaatcagtta acgttgctga aatacaatgc cggacttgcc gatccgcaga
     6781 tccaggcgaa aggcgatacg ctgtatgtta ccggcgagca ggtgaaatat cgtgattcgc
     6841 gcgaagggat catccgtgct aatcggatcg tgatgaacga tctgccggat gggatcaaaa
     6901 cgatccgcat tacggaaaat cgccttaaca tgccgcaggt gacgacggaa accgatgtcg
     6961 ccagcctgaa aaatcatctc gccggagagc cgttgggcca cgaaacgacg ctggcgcaaa
     7021 aacgcgtcga gccagtggtt ccgcagtcca ccgagcaggg ctggtatatc gacaaatcac
     7081 gctttgattt ccatatcgat ccggtgctga accagtcggt cggtggcccg gaaaactttt
     7141 acatgtatca gctgggcgtg atgggaacgg cagatttgtg gctgacggac catctgctga
     7201 ccatcggcag cctgtttgca aatcttgcca acaactacga caagtttaac tacaccaatc
     7261 cgccacagga ctcgcactta ccgcgcgttc gtacccatgt gcgcgagtat gtgcagaacg
     7321 atgcctatgt gaataacctg caagccaact acttccagca tctgggcaac ggcttctacg
     7381 gtcaggtcta tggaggttat ctcgaaacca tgtttggcgg tgcgggggct gaagtgttgt
     7441 atcgtccgct ggacagcaac tgggcgtttg gtctggatgc caactacgtt aaacagcgcg
     7501 actggcgtag tgcaaaagat atgatgaaat tcaccgacta cagcgtgaaa accggacatc
     7561 tgaccgccta ctggacgcca tctttcgccc aggacgtgtt agtgaaagcc agcgtcgggc
     7621 agtatctggc aggggataaa ggcggcacgc tggagatcgc caaacgcttt gatagcggcg
     7681 tggtggtggg tggctatgcc acgatcacga atgtttcaaa agaggagtac ggcgaagggg
     7741 acttcaccaa aggcgtgtat gtctcggtac cgttggatct cttctcgtct ggcccgacac
     7801 gcagccgtgc ggcgattggc tggacgccgc tgacgcgtga cggtggtcag caacttgggc
     7861 gtaagttcca gttgtatgat atgaccagcg accgtagcgt caatttccgc taagtcatgg
     7921 gaaaggtgcc agttttcgca atcactggca ccctcattct taatggcctg ttgagccatc
     7981 aggaacgcag ttcacccccg gtttgcgatc tggtgtgtaa aggcgaaaac attctgtctt
     8041 cctgtccgtc gatatcggtt gcaggtctgg ttgcctgagc gactgggcca tcattgggct
     8101 ggagacattg ctttgcggta ggtcggcttt atcagcagta ccgagcggac cagcataagc
     8161 aggaagaaca gagactgata acatcaaagc agcaaaataa ggcttcattt ttaccacctt
     8221 tatcaggtta cgtttcattt gttccagagg aacattgtcg atttttcgcg cattgctggt
     8281 ggctgggaat cacctgaatg ggtgattttt gaattaccgg cttgggtgcg gtttgtcttg
     8341 ccggatgcgc cgccaggcgc ggcttatccg gcctacgggt aggtatatcc ggctttggtg
     8401 gaggcgcgct ccaaatccag gttgaacaaa acatacacaa aaaatataga tctccgtcac
     8461 atttttgcgt tatacaggaa gctcgccact gtgaaggagg tactgctatg acgtcactct
     8521 ctcgtccgcg cgtggagttt atctccacca ttttgcagac cgtactcaat cttggcctgc
     8581 tgtgcctcgg cctgattttg gttgtcttcc tcggcaaaga aacggtgcat ctggcagatg
     8641 tgctgttcgc gccagaacaa accagcaaat atgagctggt agaaggactg gtggtttact
     8701 ttctctattt cgaatttatc gcgctgattg tgaagtactt tcagtccggt tttcacttcc
     8761 cgctacgtta ctttgtctat attgggatca ccgccattgt gcgcttgatc atcgtcgatc
     8821 ataaatcgcc actggatgtg ttgatctact cggctgcgat cctgttactg gtgatcactc
     8881 tgtggctgtg taattcgaaa cggctgaagc gggagtaaaa agaaagcacg ccgaactggc
     8941 gcggcgtgct ggacaggaag attacagcgt agcagtttgt tgtgttttct tcgtttccgg
     9001 ttcccagagg gcttccagct cctcaagggt tttacctttg gtttccggga caaatttcca
     9061 cataaatagt gctgccagaa cgcccataca cccgtaaatc cagtaggaga aaccgttgtg
     9121 gaaatgggcc accagccagg agtttttgtc catcatcggg aaggtccagg agacgaagta
     9181 gttcgccagc cactgggccg ccaccgcgat tgccagcgct ttaccacgaa tagcattcgg
     9241 gaagatttcc gacagcagta cccagcatac cggaccccag gacatggcaa aggcagcaac
     9301 atagaacagc atcgacagta gcgccacaat acccggtgcc tgagtgtaaa acgcggtacc
     9361 gaggctaaac ataccgattg ccattccgag tgcgccgata atttgcagtg gcttacgacc
     9421 aaatttatcc accgtcataa ttgccagaac ggtgaaggtg aggttgataa ctccgacaat
     9481 aatggtctgc aacagcgcga tatccgtgct ggcccccagc gttttgaaca cttccggcgc
     9541 gtagtacagc accacattga tgccgacaaa ttgctggaag atggagagca taacgccgat
     9601 gacaatcacg cccacgccaa acatcagcag acgaccaccg gttttgcggc catgatccag
     9661 ggagtgttta atttcctgta ctgcctgagt tgcaagcgtg ttgcccataa ttttgcgcag
     9721 gatactttcc gcctgttctt gcttgccgcg tgacatcagc cagcgaggac tttctggcac
     9781 ggtatacagc agcattaaga acagcagtgc agggatacat tccgaggcaa acatataacg
     9841 ccagccgtca gtattcagcc agctggcatc accggaacgg gcaataaaat agtttacgca
     9901 gtaaactaaa agttgcccga aaataatcgc aaactggtta aatgaaacca gttttccgcg
     9961 aatatgagct ggagccagtt ccgcaatata cattggtgaa agcattgagg ctaaaccaac
    10021 gccaataccg ccaataatac gataaataac aaattccggg acataacctg ccagataaat
    10081 aggtactgtg ttgtccgggt ttatagaggt aaaaccaagt tctggccagg cagaacctac
    10141 accagaaata aaaaacagga cagcagcaat cttaagtgaa tcacgacggc cgaagcggtt
    10201 actgcaataa ccaccgaggg caccgccgat gatgcaacca atcagagcgc tggctacgca
    10261 aaatcctaac agggagttgg cagcggattc acttaagttt tgtggagcaa caaagacggt
    10321 attgagtgac tcaacagtac cggaaataac ggcggtgtcg tagccaaata ataaaccacc
    10381 taatgtagcg actaaggtaa tcgaaaatat ataactggaa ttatactggg tattcattca
    10441 gacctgcctt agaccattct gatcttttat cagtgcattg atgttggaat tgtcaggcag
    10501 ttaaggcaat tatcattttt tacaatgcgt ttacgtaatg tcttttctgt gatcttaatt
    10561 gtgataatta tcaaaaattg atgaaaacaa tactattgcc gtgactcaga gcacgaaaga
    10621 gaattatcgt aagtgggaaa acaaataacg taaaaataat aagctctatg atggaaatat
    10681 taaccggcga acgattcaga ttgcagacga aagaaaaaaa ggcgctccgt ggagcgccga
    10741 ataacagtca caagttggga taacgtaagt tgagggtgca gcggcataac attggcagaa
    10801 caacatcttt aacctttcac accacctgcc gtcaggccgt tgaccagcca gcgttgagcc
    10861 agcaagaaga cgatggtgat cggtaatgca gacatcacgg cagcggcggc aaagtcaccc
    10921 cacaggtagt tttgcgggtt gaggtattgc tgcatcccca cggccagggt gtagctgttt
    10981 acgtcacgca gtaacagcga cgcgaccgga acttcagtaa tggcagcgat aaacgacagg
    11041 atgaatacca ccgccagaat cggtactgac agcggcaaca ggacaaggcg gaaggcctgc
    11101 cacggtgtcg caccatccag cgcagccgct tcttccagcg aactatcgat ggtttcgaaa
    11161 tagcctttga tggtccagac atgcagcgca atcccaccca gatacgcgaa aattacgccg
    11221 ccgtgagtat tcaggccaat gaatggaatg tactcaccca gacgatcaaa caacgcatac
    11281 aacgcgacca gtgaaagtac tgccgggaac atctggaaaa tcagcattcc tttcagcagc
    11341 gtcgctttgc ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt agagagcgcg
    11401 acaatgccaa tcgcggaaat cccggcgacc tttaccgagt tccatagcca cagcagtacc
    11461 gggaatggcg gtggcgtaat gcgaccatca gcctgttcca cgctaaaacc taaagccagt
    11521 ttccagtgat cccaggagat ttgctccggg atcaggctgc cgatcgcaaa gttcccctgg
    11581 cgcagtgaga tagcgacgac catcagcagc gggaacataa tcgccgcgat aaaaagtagc
    11641 agtagcaggt gagtaataaa caaacgtgct ttttgcgatt tcggttggac cattgccatt
    11701 tttgttatct cccttaatca aacttcattc gcgtggcttt caggttcact atcgccagcg
    11761 cacccaccag caggaagagc agcgtggcaa ttgctgcagc cagaccgaag tcctgacccc
    11821 cgccgccttc aaaagcgata cggtaggtgt agttaacaag caggtcggta taaccggctg
    11881 gcgtggtcgt gccaagacga tccgggccgc cgttggttaa cagttgaatc agcacgaagt
    11941 tgttaaagtt aaaggcgaag ctggcgatca tcagcggcgt cagcggttta atcagcagcg
    12001 gcagcgtaat cttaaagaag ttctggaacg gacctgcgcc atccattgct gaggcttcat
    12061 acaaatcgtc cggaatcgct ttcagcaagc ccatgcagag gatcatcatg tacggataac
    12121 ccagccaggt attgacgata attagcatcg tgcgggcggt ggtcggatcg ctgaaccagg
    12181 cgggcttcac gccaaacagc gcgctcaaca tcatgttgat ttcaccgaag ctctggttaa
    12241 acaacccttt gaaaatcaag attgaaatga atgatggcac cgcgtagggc agaatcagca
    12301 gaacgcgata gaccgctttg ccgcgcaatg cttcccactg caccagacat gccagaacca
    12361 tgccgaccgc caccgttaaa aagacagtga tcagcgagaa caccacggtc cagacgaaaa
    12421 tggcgaggaa cggtttctga atgccttcgt cggtgaagac gcgggtaaag tttttccagc
    12481 cagtagtcac ggtgtaaccg gggcttagct tttcatcacc ccagttgccg tcggcggtaa
    12541 tggactggta aaagccaatt tggttattcg gacgatattt cacgccgctc tgattattcg
    12601 tcaacgtgcc gtcaccgtcg agtgtgtaga gcggctgcgt gccagaaaac tggcgcaggg
    12661 agctcatcat cactttgttg ccatccggca gaatggcggt aatgtcactc agcgcctgac
    12721 gattctgggt aatcacgcgt agattcgcgc gttcgccttc gggctgggcg gtcgtttctt
    12781 tcagttgcag tttttgttcg ccgccaaatt taaacgcgtc ggagaggtaa tttttgccga
    12841 tttcgccgtc actgagcgcc agttgccact catcgcccgc cgggtaaaga ccaaagttat
    12901 aggttttgcc tgcttgccag gagcgatcta acaacacttc ctgcgcacat tcaaaagtca
    12961 gctggttagt gctgctgtag ttggtgaagg caatggcgat ggtgcagacc agagggaaga
    13021 ggacgaataa tcccattcca gccattcccg ggtaaacata gcgccaggcg taggctttac
    13081 gattggcgaa aatatacagc cccgctgaac tcaatatcag cgtggtaatg gcgaacaggt
    13141 attccccttg tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg agcagaccta
    13201 gcactgacca tttcagcgcg tcgctttgcc accaatgttt ctttttaatg acatccatgg
    13261 ggttcttcct cattccagga cggataaggc tttcacgcct tatccgacaa caactgcctg
    13321 atgcgacact gatgcgtctt atcaggccta catgcgtttc agttttgtag gccggacaag
    13381 gcgttcacgc cgcatccggc atttcacagc attacttggt gatacgagtc tgcgcgtctt
    13441 tcagggcttc atcgacagtc tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg
    13501 cataccagaa agcggacatc tgcgggatgt tcggcatgat ttcacctttc tgggcgtttt
    13561 ccatggtggc ggcaatacgt ggatctttcg ccaactcttc ctcgtaagac ttcagcgcca
    13621 ctgcacccag cggtttgtct ttattgaccg cttccagacc ttcgtcagtc agcagatagt
    13681 tttcgaggaa ctctttcgcc agctctttgt tcggactggc ggcgttaata ccggcgctca
    13741 gcacgccaac gaacggttta gatggttgac ccttgaaggt cggcagtacc gttacaccat
    13801 aattcacttt gctggtgtcg atgttggacc atgcccacgg gccgttgatg gtcatcgctg
    13861 tttcgccttt attaaaggca gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt
    13921 tgtttttaat aaggtcaacc aggaaggtca gacccgcttt cgcgccagcg ttatccacgg
    13981 ccacgtcttt aatatcgtac ttgccgtttt catacttgaa cgcataaccc ccgtcagcag
    14041 caatcagcgg ccaggtgaag tacggttctt gcaggttgaa catcagcgcg ctcttacctt
    14101 tcgctttcag ttctttatcc agcgccggga tctcttccca ggtttttggt gggttcggca
    14161 gcagatcttt gttataaatc agcgataacg cttcaacagc gatcgggtaa gcaatcagct
    14221 tgccgttgta acgtacggca tcccaggtaa acggatacag cttgtcctgg aacgctttgt
    14281 ccggggtgat ttcagccaac aggccagatt gagcgtagcc accaaaacgg tcgtgcgccc
    14341 agaagataat gtcagggcca tcgccagttg ccgcaacctg agggaatttc tcttccagtt
    14401 tatccggatg ctcaacggtg actttaattc cggtatcttt ctcgaatttc ttaccgactt
    14461 cagcgagacc gttatagcct ttatcgccgt taatccagat taccagttta ccttcttcga
    14521 ttttggcgag agccgaggcg gaaaacatca tcgtcgttaa tgcggataat gcgaggatgc
    14581 gtgcacctgt ttttattttc ataatctatg gtccttgttg gtgaagtgct cgtgaaaaca
    14641 cctaaacgga ctctagtttc tttatacggc aacctctttc catcctcctt gcccctacgc
    14701 cccaccgttg ctttgtgtga tctctgttac agaattggcg gtaatgtgga gatgcgcaca
    14761 taaaatcgcc acgatttttg caagcaacat cacgaaattc cttacatgac ctcggtttag
    14821 ttcacagaag ccgtgttctc atcctcccgc ctcctccccc ataaaaaagc cagggggtgg
    14881 aggatttaag ccatctcctg atgacgcata gtcagcccat catgaatgtt gctgtcgatg
    14941 acaggttgtt acaaagggag aagggcatgg cgagcgtaca gctgcaaaat gtaacgaaag
    15001 cctggggcga ggtcgtggta tcgaaagata tcaatctcga tatccatgaa ggtgaattcg
    15061 tggtgtttgt cggaccgtct ggctgcggta aatcgacttt actgcgcatg attgccgggc
    15121 ttgagacgat caccagcggc gacctgttca tcggtgagaa acggatgaat gacactccgc
    15181 cagcagaacg cggcgttggt atggtgtttc agtcttacgc gctctatccc cacctgtcag
    15241 tagcagaaaa catgtcattt ggcctgaaac tggctggcgc aaaaaaagag gtgattaacc
    15301 aacgcgtcaa ccaggtggcg gaagtgctgc aactggcgca tttgctggat cgcaaaccga
    15361 aagcgctctc cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg gtggccgagc
    15421 caagcgtatt tttgctcgat gaaccgctct ccaacctcga tgctgcactg cgtgtgcaaa
    15481 tgcgtatcga aatctcccgt ctgcataaac gcctgggccg cacaatgatt tacgtcaccc
    15541 acgatcaggt cgaagcgatg acgctggccg acaaaatcgt ggtgctggac gccggtcgcg
    15601 tggcgcaggt tgggaaaccg ctggagctgt accactatcc ggcagaccgt tttgtcgccg
    15661 gatttatcgg ttcgccaaag atgaacttcc tgccggtaaa agtgaccgcc accgcaatcg
    15721 atcaagtgca ggtggagctg ccgatgccaa atcgtcagca agtctggctg ccagttgaaa
    15781 gccgtgatgt ccaggttgga gccaatatgt cgctgggtat tcgcccggaa catctactgc
    15841 cgagtgatat cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag caactcggca
    15901 acgaaaccca aatccatatc cagatccctt ccattcgtca aaacctggtg taccgccaga
    15961 acgacgtggt gttggtagaa gaaggtgcca cattcgctat cggcctgccg ccagagcgtt
    16021 gccatctgtt ccgtgaggat ggcactgcat gtcgtcgact gcataaggag ccgggcgttt
    16081 aagcacccca caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga aaagcaatga
    16141 ctcaggagat agaatgatga ttactctgcg caaacttcct ctggcggttg ccgtcgcagc
    16201 gggcgtaatg tctgctcagg caatggctgt tgatttccac ggctatgcac gttccggtat
    16261 tggctggaca ggtagcggcg gtgaacaaca gtgtttccag actaccggtg ctcaaagtaa
    16321 ataccgtctt ggcaacgaat gtgaaactta tgctgaatta aaattgggtc aggaagtgtg
    16381 gaaagagggc gataagagct tctatttcga cactaacgtg gcctattccg tcgcgcaaca
    16441 gaatgactgg gaagctaccg atccggcctt ccgtgaagca aacgtgcagg gtaaaaacct
    16501 gatcgaatgg ctgccaggtt ccaccatctg ggcaggtaag cgcttctacc aacgtcatga
    16561 cgttcatatg atcgacttct actactggga tatttctggt cctggtgccg gtctggaaaa
    16621 catcgatgtt ggcttcggta aactctctct ggcagcaacc cgctcctctg aagctggtgg
    16681 ttcttcctct tttgccagca acaatattta tgactatacc aacgaaaccg cgaacgacgt
    16741 tttcgatgtg cgtttagcgc agatggaaat caacccgggc ggcacattag aactgggtgt
    16801 cgactacggt cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg catcgaaaga
    16861 cggctggtta ttcactgctg aacatactca gagtgtcctg aagggcttta acaagtttgt
    16921 tgttcagtac gctactgact cgatgacctc gcagggtaaa ggtctgtcgc agggttctgg
    16981 cgtcgcgttt gataacgaaa aatttgccta caatatcaac aacaacggtc acatgctgcg
    17041 tatcctcgac cacggtgcga tctccatggg cgacaactgg gacatgatgt acgtgggtat
    17101 gtaccaggat atcaactggg ataacgacaa cggcaccaag tggtggaccg tcggtattcg
    17161 cccgatgtac aagtggacgc caatcatgag caccgtgatg gaaatcggct acgacaacgt
    17221 cgaatcccag cgcaccggcg acaagaacaa tcagtacaaa attaccctcg cacaacaatg
    17281 gcaggctggc gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa cctacgccaa
    17341 gtgggatgag aaatggggtt acgactacaa cggcgatagc aaggttaacc cgaactacgg
    17401 caaagccgtt cctgctgatt tcaacggcgg cagcttcggt cgtggcgaca gcgacgagtg
    17461 gaccttcggt gcccagatgg aaatctggtg gtaatagcaa aacctgggcc ggataaggcg
    17521 tttacgccgc atccggcata aaaacaggtt gtcattatct gaaaggggcg aaagcccctc
    17581 tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat tttgaggtga
    17641 aaacaatgaa aatgaataaa agtctcatcg ccctctgttt atcagcaggg ttactggcaa
    17701 gcgcgcctgg aattagcctt gccgatgtta actacgtacc gcaaaacacc agcgacgcgc
    17761 cagccattcc atctgccgcg ctgcaacaac tcacctggac accggtcgat caatctaaaa
    17821 cccagaccac ccaactggcg accggcggcc aacaactgaa cgttcccggc atcagtggtc
    17881 cggttgctgc gtacagcgtc ccggcaaaca ttggcgaact gaccctgacg ctgaccagcg
    17941 aagtgaacaa acaaaccagc gtttttgcgc cgaacgtgct gattcttgat cagaacatga
    18001 ccccatcagc cttcttcccc agcagttatt tcacctacca ggaaccaggc gtgatgagtg
    18061 cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa aaactttatg
    18121 ttctggtctt taccacggaa aaagatctcc agcagacgac ccaactgctc gacccggcta
    18181 aagcctatgc caaaggcgtc ggtaactcga tcccggatat tcccgatccg gttgctcgtc
    18241 ataccagcga tggcttactg aaactgaaag tcaaaacgaa ctccagctcc agcgtgttgg
    18301 taggaccact atttggttct tccgctccag ctccggttac ggtaggcaac acggcggcac
    18361 cagctgtggc tgcacctgct ccggtgccgg tgaagaaaag cgagccgatg ctcaacgaca
    18421 cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat gttgataagg
    18481 cgttaaaact gcttgatgaa gctgaacgcc tgggatcgac atctgcccgt tccaccttta
    18541 tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg caacaactgg
    18601 tgcgtctcct ggcgcacctt tttttatgct tccttcctgg gatatgagcg attttttata
    18661 gtacttcact tcttcttcac taagaatgtc cattatctca atgccttatc agagattctt
    18721 ttcctttcgc cggtagtgtc tggacgctca ggctactttt ccaggttatt ttatttctgt
    18781 attacagagg ttttatgata ggtcatatca caaattctgg cggcaataac tctttgatga
    18841 aacatga
//
LOCUS       DAZOZY010000069        18513 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZOZY010000069 DAZOZY010000000
VERSION     DAZOZY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.069"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            76..288
                     /gene="cspH"
                     /locus_tag="ACHURQ_002078"
     CDS             76..288
                     /gene="cspH"
                     /locus_tag="ACHURQ_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR0823855.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            735..1040
                     /locus_tag="ACHURQ_002079"
     CDS             735..1040
                     /locus_tag="ACHURQ_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823856.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHURQ_002080"
     CDS             1147..1791
                     /gene="gfcB"
                     /locus_tag="ACHURQ_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR0823857.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1788..2534
                     /locus_tag="ACHURQ_002081"
     CDS             1788..2534
                     /locus_tag="ACHURQ_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR0823858.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2534..4630
                     /locus_tag="ACHURQ_002082"
     CDS             2534..4630
                     /locus_tag="ACHURQ_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR0823859.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4676..5815
                     /locus_tag="ACHURQ_002083"
     CDS             4676..5815
                     /locus_tag="ACHURQ_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR0823860.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5803..6249
                     /gene="etp"
                     /locus_tag="ACHURQ_002084"
                     /gene_synonym="yccY"
     CDS             5803..6249
                     /gene="etp"
                     /locus_tag="ACHURQ_002084"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR0823861.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6269..8449
                     /gene="etk"
                     /locus_tag="ACHURQ_002085"
     CDS             6269..8449
                     /gene="etk"
                     /locus_tag="ACHURQ_002085"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR0823862.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHURQ_002086"
     CDS             complement(8564..9862)
                     /gene="appA"
                     /locus_tag="ACHURQ_002086"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR0823863.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHURQ_002087"
     CDS             complement(9942..10034)
                     /gene="cbdX"
                     /locus_tag="ACHURQ_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR0823864.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHURQ_002088"
     CDS             complement(10047..11183)
                     /gene="appB"
                     /locus_tag="ACHURQ_002088"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR0823865.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHURQ_002089"
     CDS             complement(11195..12739)
                     /gene="appC"
                     /locus_tag="ACHURQ_002089"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR0823866.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12873..13730)
                     /locus_tag="ACHURQ_002090"
     CDS             complement(12873..13730)
                     /locus_tag="ACHURQ_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR0823867.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHURQ_002091"
     CDS             complement(13787..14125)
                     /gene="hyaE"
                     /locus_tag="ACHURQ_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR0823868.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHURQ_002092"
     CDS             complement(14122..14709)
                     /gene="hyaD"
                     /locus_tag="ACHURQ_002092"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR0823869.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHURQ_002093"
                     /pseudo
     CDS             complement(14706..15412)
                     /gene="hyaC"
                     /locus_tag="ACHURQ_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHURQ_002094"
     CDS             complement(15431..17224)
                     /gene="hyaB"
                     /locus_tag="ACHURQ_002094"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR0823870.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHURQ_002095"
     CDS             complement(17221..18339)
                     /gene="hyaA"
                     /locus_tag="ACHURQ_002095"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR0823871.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4547 a         4914 c         4782 g         4270 t
ORIGIN      
        1 gcggtcttaa atatcgctgg ctttatttta ttaaccccgt atagtgcagc gcagtatttt
       61 cagtaaggaa tttgtttgtc ccgtaaaatg acaggaattg tcaaaacctt tgatcgcaaa
      121 agcggcaaag gattcattat cccctccgac ggtcgtaaag aagttcaggt ccatatttcc
      181 gcattcactc cccgcgacgc agaagtgctt ataccaggat tacgtgtgga attttgccgc
      241 gtaaatggcc tgcgaggacc aacagcggca aatgtttatc tctcttaata tgacgctgct
      301 tgtttaaagc tgactggttt tattggaata gtgcaatagt caacatcata cattataaca
      361 tcagttactt ttccacaaag aaactaatcc atttaagcga atatctctgc acctaatagt
      421 tataaatgcc atttaacagc cagttatagt accacttgaa actatattac caacaataat
      481 taatagcttt cgtaacaatt tccttatctt taatttgtat tttgctaaga tttatcttat
      541 gctcacaagt cgtatttcca gaggggatcg ttaataatct tatatttccc tctgcgcacc
      601 tttcctaccc gcagtatcag tatatagtgc aagcggtatg aggaaaggga gatttcacaa
      661 ggatgttata ctcaaagtag acaatcctga tctcaagcgt acgtattgtc catgcgatga
      721 ataaaggaaa agttatgaaa cacaagcttt ctgcaatcct tatggccttc atgttgacga
      781 cgccagctgc gtttgctgcc tctgaagcaa caaacgggac cgaggcaacg acgggtacta
      841 ctggcaccac aacgaccact accggcgcaa ccacgactgc tactaccact ggtggcgtgg
      901 ctgctggcgc tgtcggcacg gcgacagttg tcggtgtggc aacggctgtt ggtgtggcga
      961 cgttggcagt tgttgcagca aatgacagtg gcgatggcgg ttctcataac acctcaacca
     1021 ccacgtccac tacacgctaa tactgcttta acgcgtcgaa attaaagtta aatgataccc
     1081 ggttgcgata ttcgtaccgg gctttagaga tttgcatttt tttaattaac ctttcaagga
     1141 ttaaaggtgc gccctcttat tttatcgatt ttcgcactat ttcttgcggg atgtacgcac
     1201 agccagcaaa gtatggtcga tacatttcgc gccagccttt tcgataatca ggatatcacc
     1261 gtagcggatc agcagatcca ggcgttgcct tattccacga tgtatttacg ccttaatgaa
     1321 gggcaacgaa tctttgtggt actgggatat atagaacaag aacaaagcaa atggttatcc
     1381 caggataacg ccatgctggt tacccacaat ggacgtcttt taaaaaccgt caaacttaat
     1441 aataatctgc tggaagtgac aaattccggg caggaccctc tgcgtaacgc gctggcaata
     1501 aaagatggca gccgctggac gcgcgatatt ctctggagtg aagacaacca ttttcgctct
     1561 gcgaccctga gttctacttt ttcctttgct ggattagaga cgctgaatat tgcgggtcgc
     1621 aatgtgctgt gtaatgtctg gcaggaagag gtgacttcca cgcggccaga aaaacagtgg
     1681 caaaacacat tctgggtcga ttcggctact ggccaggttc gtcaaagtcg gcaaatgtta
     1741 ggcgcagggg ttattcccgt agaaatgacg tttcttaaac ccgcaccatg aataaattac
     1801 agtcgtattt cattgccagc gtactttacg taatgacacc ccatgccttt gcgcaaggaa
     1861 cggtgactat ttatctgcct ggcgaacaac agacactttc cgttggcccc atggaaaatg
     1921 ttgtccagtt ggtgacacaa ccgcaactgc gcgatcgcct ctggtggcct ggtgccttgc
     1981 tgaccgactc tgcggcgaaa gctaaagcgc ttaaagacta ccaacatgtc atggcacagc
     2041 ttgcttcctg ggaagcagaa gctgacgacg atgtggcggc aacgattaaa tccgtgcgcc
     2101 agcagttact caatctcaat atcactggtc gcttaccggt caaactggac cctgattttg
     2161 tacgagtgga tgagaacagc aatcccccgt tggtcgggga ctatacgctc tataccgtac
     2221 agcgtccggt gactattact ctgctgggtg cggtttctgg ggccgggcaa ttgccctggc
     2281 aggcaggccg tagcgttacc gattatctgc aagatcaccc ccgccttgca ggcgcggaca
     2341 agaataacgt tatggtgatt acgcctgagg gtgagactgt cgtcgcccct gttgccctgt
     2401 ggaataaaag acatgtggaa ccaccgcctg gcagccagct ttggttgggc ttttctgcgc
     2461 atgtactgcc cgaaaaatat gctgatttaa atgaccagat tgtgtctgtt ttaacgcaac
     2521 gtgttcctga ctgatgaaga agaattctta tcttttaagc tgcctggcca ttgccgtctc
     2581 cagtgcctgt catgctgaag tattaaccta cccggatccg ctgggttcgt cgcaatcaga
     2641 ctttggcggc acaggattgt tgcagatgcc aaatgcgcgc atcgcaccgg aaggtgaatt
     2701 cagcgtcaac taccgggata acgatcaata ccggttctac tccacctccg tggcgctgtt
     2761 cccatggctg gaaggcacca ttcgttatac ggatgtgcgc acacgcaaat atagccagtg
     2821 ggaagatttc agcggcgatc agtcatacaa agacaaatca ttcgatttta aacttcgcct
     2881 gtgggaagaa ggttactggc taccgcaagt ggcgtttggt aaacgtgata ttgctggtac
     2941 gggtctgttt gacggtgagt atctggtggc cagcaagcaa gcggggccat ttgatttcac
     3001 cctcgggatg gcatggggct acgccggtaa tgcgggcaat attaccaacc cgttttgccg
     3061 ggtgagcgat aaatattgtc atcgcgcaga gtctcacgat gcgggcgata tcagctttag
     3121 cgatatcttt cgtggcccgg cttccatctt tggcggcatt gagtatcaaa cgccgtggaa
     3181 tcccctgcgt ctgaaactcg aatacgatgg caacaattac cagaatgatt tcgctggcaa
     3241 actgcctcag gcaagccatt tcaacgtcgg cgcagtttat cgcgctgcca gctgggcaga
     3301 tctcaacctg agttatgaac gcggtaacac gttgatgttt ggcttcacgt tacggaccaa
     3361 tttcaacgat ctgcgccctg ccctgcgcga tacgccaaaa ccggcatatc aacctgcgcc
     3421 tgaatctgaa ggattgcagt acaccacagt agcgaaccaa cttaccgcgc tgaagtacaa
     3481 cgcaggtttt gaagcaccgg aaattcagct gcgcgataag acgctgtata tgtctggtca
     3541 acaatacaaa taccgtgatt ctcgcgaagc ggtcgatcgt gccaaccgga ttctggtgaa
     3601 taacctgccg caaggcgttg agaagattag cgtgacgcaa aagcgcgagc atatggcgat
     3661 ggtgactacc gaaaccgacg tagccagcct gcgcaaacag ctggctggta cagcgcctgg
     3721 tcaatcagag caacttcaac aacaacgtgt tgaagcagaa gatctttctg cctttggtcg
     3781 gggctaccgt attcgtgaag atcgctttag ctactctttc aacccaacac tttcacagtc
     3841 gctgggcggc ccggaagatt tctatatgtt ccagctgggg ctgatgtcca gtgcccgcta
     3901 ctggtttacc gaccacctgc tgcttgatgg cggtattttc accaatattt acaacaacta
     3961 cgacaagttt aagtcttcgc agttgcccgc ggactctacc ctgccccgcg tgcgcacgca
     4021 tatccgtgat tacgttcgca atgacgttta tctcaacaac ttgcaggcga actactttgc
     4081 cgacttaggc aatggtttct atggccaggt gtatggcggt tatctggaaa cgatgtacgc
     4141 cggtgtcggt tccgagctgc tttatcgccc gctagatgcc agctgggcgc tgggtgtgga
     4201 cgttaactac gtgaagcaac gtgactggga caacatgatg cgcttcaccg attattccac
     4261 gccaactggt ttcgtgacgg cttactggaa cccgccgacg ctcaatggcg tactgatgaa
     4321 acttagcgtt gggcaatatc tggcaaaaga taaaggggca acgatcgacg tcgccaaacg
     4381 ctttgacagc ggcgtggcgg taggggtatg ggcggcaatc agtaacgtat ctaaagatga
     4441 ctacggcgaa ggcggcttta gtaaaggttt ttatatctcg attccgttcg acttgatgac
     4501 cattggacct aaccgcaacc gcgcggtggt ttcgtggaca ccattgacgc gtgatggtgg
     4561 acaaatgctg tcacgcaaat accagctcta tccaatgacg gcagagcgag aagtaccggt
     4621 tggacaataa cctgacgtaa ttcataacat tacattgact gataaatata aaccaatgaa
     4681 aaagaacatt tttaagtttt cggtactgac attggctgtt ctgtctttga cagcatgtac
     4741 ccttgttcct ggtcagaatt tatcgaccag taacaaggat gtcattgaac tacctgacaa
     4801 tcagtatgac ctggacaaaa tggtcaatat ctatcccgtc acgccaggat tgattgatca
     4861 gttgcgcgcc aaaccgatta tgtcgcaggc taacccggag ctggagcagc aaatcgcaaa
     4921 ctatgaatat cgtatcggga ttggtgatgt gttgatggtt acggtctggg atcacccgga
     4981 actaaccacc cccgcagggc aataccgtag cgccagtgat accggcaact gggttaacgc
     5041 cgatggtgcg attttctacc cctatattgg tcgcctgaaa gtggccggga aaacacttac
     5101 ccaggtacgc aacgagatta cagcgcgtct cgattcagtc attgaaagcc cgcaagtcga
     5161 tgtcagcgtc gcggcgttcc gatcgcaaaa agcgtacgtc accggcgaag tcagcaaatc
     5221 gggtcagcag ccgattacca atattcctct gaccatcatg gatgcgatta acgccgcggg
     5281 cggtctgacc gcagatgcag actggcgcaa cgtggtgctc acccagaatg gcgtaaagac
     5341 caaagtgaac ctctatgcgc tgatgcagcg tggcgacttg cgccagaaca aactgctgca
     5401 cccgggcgat attttattta ttccgcgcaa cgatgatctg aaagtcttcg tgatgggtga
     5461 agtgggtaaa cagagcacgc tgaaaatgga ccgcagcggt atgaccctgg cggaagcgct
     5521 gggtaacgct gagggtatga atcaggatgt cgctgatgcg acggggattt ttgttattcg
     5581 tgcaacgcaa aacaagcaga atggcaaaat cgccaatatt tatcagctga atgcgaagga
     5641 cgcatcggca atgatactcg gaacggaatt ccagcttgaa ccttacgaca ttgtttatgt
     5701 cactaccgcg ccgctggctc gttggaaccg tgtgatttct ctgctggtac cgacgatttc
     5761 cggggtgcat gacctgacag agacttcacg gtggattcaa acatggccca actaaaattt
     5821 aactcaatcc tggtggtttg taccggcaac atttgccgtt cgcccattgg cgagcgcttg
     5881 ctgcgtaaac gtctgccggg cgtaaaagtc aaatcggcag gtgttcatgg tctgataaaa
     5941 caccctgcag atgcgacagc ggcagatgtt gccgctaatc atggcgtatc tcttgaagga
     6001 catgccggac gtaagctcac tgctgagatg gcacgaaatt acgatctgat tctggcaatg
     6061 gagtcggaac atattgctca ggttaccgca attgctcccg aggtccgcgg gaagacaatg
     6121 ctgtttgggc aatggctgga acagaaagag atcccggatc cctatcgtaa aagtcaggac
     6181 gcatttgaac atgtctacgg tatgttggag cgcgccagtc aggaatgggc gaagcgcctc
     6241 agccgataat ttgagtttat aaaatacgat gacaactaaa aatatgaata cgccaccagg
     6301 cagcactcag gaaaatgaga tcgatctgct tcgtctggtc ggcgagttat gggatcaccg
     6361 taagtttatt atcagcgtga ccgcgttatt cacgctgatc gctgtcgctt actcgctgtt
     6421 aagcacacca atttatcagg cagatactct ggtccaggtt gagcaaaaac agggcaacgc
     6481 cattctcagc ggcctgagcg atatgatccc taactcatcg cccgagtctg caccggagat
     6541 ccaactgctg caatcgcgca tgattctcgg taaaaccatt gctgaactga atctgcgcga
     6601 catagttgag cagaagtatt ttccgattgt gggtcgcggc tgggcgagat taaccaaaga
     6661 aaaaccaggt gagctggcga tcagctggat gcatattcca caactgaatg gtcaggatca
     6721 gcaactgaca ctcacggttg gggaaaacgg ccactataca ctggaaggtg aagagttcac
     6781 cgtcaatggt atggtcggcc agcgtctgga aaaagatggc gttgcgctga ctatcgcgga
     6841 cattaaggcc aaaccaggaa cacagtttgt cctgagccag cgtaccgaac tggaagcgat
     6901 taacgcattg caggaaacct ttaccgttag cgaacgcagt aaagaaagcg ggatgctgga
     6961 acttaccatg actggtgatg atccccagtt gattactcgt attctgaaca gcatcgctaa
     7021 caactatttg caacagaata tcgctcgcca ggcggcgcag gattcacaaa gccttgaatt
     7081 cttacagcgc cagttacctg aagtgcgcag cgagctggac caggcggaag aaaaactcaa
     7141 cgtttatcgc cagcagcgcg attcggttga ccttaacctg gaagccaaag ccgttcttga
     7201 gcagattgtg aacgttgata atcaactcaa tgagctgacc ttccgcgagg cagagatctc
     7261 ccagctgtat aagaaagatc acccaactta tcgtgcgctg ctggaaaaac gccagacgct
     7321 ggagcaagaa cgcaaacgcc tgaataagcg ggtatcggca atgccttcca cccaacagga
     7381 agtgttgcgt ttaagtcgtg acgtagaagc gggccgtgcg gtatatctgc aattacttaa
     7441 ccgccagcag gagttgagta tttcgaaatc cagtgccatt ggtaacgtgc ggattatcga
     7501 cccggcagtc actcagccgc agccagtgaa accgaaaaaa gcgttgaatg tggtgcttgg
     7561 ttttattctt ggcctgttta tttctgtggg tgccgtgctg gcgcgtgcga tgttgcgtcg
     7621 tggtgtagaa gccccggaac aactggaaga gcacggcatc agcgtttatg ccactatccc
     7681 aatgtccgag tggctggata aacgcacccg tctgcgtaag aaaaatttat tttctaatca
     7741 gcagcgccat cgtactaaaa atatcccctt cctggcggtg gataacccgg cggattctgc
     7801 tgtggaagcc gtacgtgcgc tacgaaccag tctgcatttc gctatgatgg agacggagaa
     7861 taacattctg atgatcaccg gtgcgacgcc agacagtggt aaaacgtttg tcagttcaac
     7921 tctggcagcg gtgatcgccc agtccgatca aaaagtgtta tttattgatg ccgacttacg
     7981 ccgtggttat tcgcataacc tgtttaccgt gagtaatgaa catggcttgt cggaatatct
     8041 ggcaggtaaa gatgagctca acaaagtgat ccagcatttt ggcaaaggag gctttgatgt
     8101 gattactcgc ggtcaggtgc cacctaaccc gtctgaactg ctgatgcgcg atcggatgcg
     8161 tcaattactg gaatgggcga acgaccatta cgatctggtg attgtcgata cgccgccgat
     8221 gctggcggtg agtgatgccg cggtcgtggg gcgttctgtt ggcaccagcc tgctggttgc
     8281 gcgttttggc ttgaacaccg ccaaagaggt gagtttgtca atgcagcgtc tggaacaggc
     8341 aggcgtcaat attaaaggcg ctatcctcaa tggtgtgatt aaacgcgcca gcaccgctta
     8401 cagttacggc tataactatt acggttatag ttactccgag aaagagtaaa actgaaagtg
     8461 gccggatgcg gcgtgaacgc cttttccggt ctacgcatta gacagttctt cgtttgtcat
     8521 cagcctcaga gcattcaggt aactgaatgc tcttttttat gcattacaaa ctgcacgccg
     8581 gtatgcgtgc ttcattcacg atttgcgtaa aacctgccaa cgaacacatg ccctgcgcat
     8641 ttcgctcttc acatcctgcc agggtcagtt tcacctctcc gggcggcgta tttaatgaca
     8701 gcggcgtttt atcacgcatc tgctgtaaag tctggaagac cagcgaaacc tgaatccact
     8761 ggctgttatc gcttagccga cgccagcgtt caaacaccag ttcaccacct ggcggcgtgt
     8821 tatccggctg accgggaagc gtccagttga gctccagtgc gccgccgaga tttgccagat
     8881 tagtatcgtg tccggcgata aacagcactg aagtgggtaa tgtcacacca tacgcctgtt
     8941 tttgcggtgg atggggcgtc aacgctgtct tgatcaaatc taataacggg gtggcgcggc
     9001 tgcgggcaac ctctggcgtg cgttgtagca aataaaattg cgcgttatgc aaacttagca
     9061 aggtgttcca ctggtgtgaa tcggtgatcc ttccccaccc cggctccggc attccctgtg
     9121 cttgttgcag gagaaatatc tccgtcagca ttgatgcgag gcttaccgca ccggttaatg
     9181 agacattgtc ggcgctcacc ttgagttccg atggtaatgc ctgcgttaat gaacagcttt
     9241 cgtcctgttt ctcacgttta aggcacaagt ttgattgcgg aaaattaagc acccgttcca
     9301 gttcgcgaaa cgccgtttgc cgatgcccgg taaagtcagc aattgaccct cctgccctgc
     9361 tgaggatcgc gtcagtcacg ttcgcgttat ccagttggca aacgccagtt tttagaggat
     9421 taaataacgg atcgggactg gacgtatcag cctgggtatg tacggttatt gcacagtcag
     9481 gtgccagccc ggcggcgaag gcttcgcctg ttttacgggt acgctcgtcg acatcagcaa
     9541 taatcgcgac ctgaccagac tgcgggcagc cctttttcgc cagcaatccg tcggctacca
     9601 gacgctggcg ttggtaatgt ccgagatagg cgattagctc accaccgcgc ggtgtcagcc
     9661 aacccagttt taccggccag gttggccatg cgtctggggt gacatcctgc atcagttgcg
     9721 tggccttggt tggagcacgc acaccatgac gactgacaat caccacactt tccagcttca
     9781 gctccggctc actctgagcg aatgcagatt gcggggttaa cggaatcaga agagataaaa
     9841 atgggattaa gatcgctttc atcgatatgt ttccgctttt catatctgac attattgatt
     9901 gcctgatgcg atgctaatgc gccgcatcag gcctacgttc gttaactttt cagacgcggg
     9961 tccagccata ccaacacaag ggtggagagc gaacacatca acaaaatgcc gacgaaccaa
    10021 agtaaatacc acattgttcc tgctccttag tacaactcgt tttcgttacg gcggagagtt
    10081 tctgttgtca tgcgtcccca cattttgtag tagctccaga gagtgtagag caacacaatg
    10141 ggcaaaaata tcagcacgat taccaacata atgctcagcg tcagctgact ggaagtgctg
    10201 tcccacaacg tcaggctgga gattggactc acgcttgacg gcatgacaaa ggggaacagc
    10261 gtgatgcctg ccgtgaaaat cacgccaaat tgcatcaatg acgccattaa aaatccccaa
    10321 cccgggcgac cacgataaat cgccatcacc gtcagcaatg ggcagaagaa ccccagcagc
    10381 gggaagatcc acaaaacggg cgattcgacg aaattattca tccaggcacc aggtagcact
    10441 gccaccagtt tcattaacgg attggaagga ccgttagcat cctgggcgag cagtacaaag
    10501 ccatcaatac cgacccacag ccagtaaccc gccagcaaaa agcacagcat caccaacagt
    10561 gcagcgcgtt tggtggccag ctgtgaacgc agatgaatca caccaacagt tttcagttgt
    10621 aaccagacgc caccttgcaa aatcaccatc ccaaggctga gcaatccgca caataaaggg
    10681 aatggcgtca gcagttgcca gaagctaccg agatactcca cgcgtaattg cggtgtgaag
    10741 gcaaacggca cgccgagcaa caagttgccg aaggcgatac cgaagactac cggcggcacc
    10801 agactgccga tgaccagacc ggcgtcccac attttacgcc agcgtgcatc ggcgattttt
    10861 ccgcgataat caaaggccag cgggcggaag aacagtgagc acagcaccag gatcatcgcc
    10921 acataaaagc cggaaaacgc cgctgcatac actctgggcc aggcggcaaa taatgcccca
    10981 ccagcgagga tcaaccagac ctggttgcct tcccagtgtg caccaacgct gtttatcact
    11041 atccggcgtt catcatcatt acgcgccacc agcggcagca gacagccgat ccccatgtca
    11101 aatccgtcgg agatcataaa gaccaccagg atcacgccaa tcagcagcca ccagatgaag
    11161 cgcaatgttt cataatcaaa catgattttc ccctttaccc ctgttgctgc gtcggttgtt
    11221 cactctgcat cgcgctcggc cccagacggg catatttctg catcaggtag acttcggcga
    11281 ttaagaacag ggtgtaaagc cctacgatca tgatcagtga gaaagccagt tgtcctgtgg
    11341 ttaaagcgga gtgcgcggag tatgtcggta agatgtcctg tatcgcccac ggctgacgac
    11401 caaactcggt cataaaccac ccggcttcaa tcgcaatcca cggcaacggc aaactccaga
    11461 gcgccatttt cagcacccag cgatgctggt cgattttgcc acgcagcgtc tggacaagcg
    11521 caatcagcat caccagtagc ggcagggaac cacagcccac catgatgcgg aaactccaga
    11581 ataccggcgc aacctgaggt atcgcgccac gcatcgccgc ctggtactgt gcggctgtga
    11641 catgattcat atccggcgca tagcgggaga gcaacatgcc gtagcccagg tcaccttcca
    11701 gttcccggaa tgcctgcaac acatgcggct cacgattgcc ttgcgatatt tcctgcatta
    11761 acagccaggc catacgtccg cgttgcaagc gtgggtaggt ttcagccatc aaatttttca
    11821 gacccggcac ggttttatct aatgagtgag tggcgaggat ccctaacagc gcgggaattt
    11881 tgatggcaaa ggcgttacgc tcttgatcct gttccggcca ggcaaccaca tggaacggtg
    11941 caggagcagg ttccgtttgc cactcccctt ccatcgccgc cagttttacc ggttgtactt
    12001 gcgcgacttc atacgcagaa ctgtctccga gttgtagcgt gccgataatc gccagagtac
    12061 cgaagacgga accgatggca aacgagcgta atgcaacatc gcgctcccgt ccgcgcagta
    12121 aataccaggc gctgatcgcc ataataaaca tggccccggt cacgtagccc gccattacgg
    12181 tgtgcacaaa tttcacctgg ctgaccgggt taaagaccag ctcgctgaag ctggtcatct
    12241 ccatacgtag ggtgtcgata tcaaaatgcg caccggtcgg gtattgcatc caaccgttgg
    12301 cattcaatat ccacaatgca gagagatttg aaccgaacgc caccagccac gtcaccagca
    12361 ggtgctggta tttattcagc cgctgccagc cgaagaagaa aagcccgaca aaggtggatt
    12421 cgaggaagaa ggccattaat gcttccatcg ccagcggtgc gccaaaaata tcgcccacat
    12481 agttggaata gaatgaccag ttagtaccaa actgaaactc catggtcagg ccggtagcca
    12541 cgccaagagc aaagttgata ccgaagagct taccccagaa gcgcgtcata tcgcggtaga
    12601 ttgttttgcc ggtgaccacg taaatggttt ccatgatagc cagcaaaaaa atcagcccca
    12661 gggtaagggg tacaaataaa aagtgataca gcgcggtcag agcaaactgc cagcgcgata
    12721 aatcaatgac atcccacatg cgcactcctg taggcgaaga gagaaaaata acccgataat
    12781 gttagatctc cctctttatc ctgaaactct acgaccagag gtattcgccg attgcgatat
    12841 tcataagact gaaagcgata cttaccgtct ttttacgtcg gtgcagcttc cgccagccac
    12901 tgacatacct cgctaagccg ctgcgcagag tcgaccaaat cttctggcgc tgccagcacc
    12961 acttccggta ttgggcagat ttcataactt tccagtaaag gaccttttaa ggtgtccgta
    13021 cagcgtaaat gccagacatg gcgtaacccc gtggcgttga tatagctctc gccatagcca
    13081 atggtacgaa tctgaatatt tcccggccca cagagacgtg agagaaaaag ccgatcagcc
    13141 tcgctgatgg gtaattgcgt cagattaatg ctgtgcggct gcgcgtcggg gttacgtaca
    13201 tgcgccagta actcatgcgc caacggtagg ccattcatca ggccatcaat gggcggcggt
    13261 aacagcgaat ctgtcggcaa gagattttgc gtggccgcct gccacaacgc cagcggcgcg
    13321 cagccggcct ccagtttgtc ctccagcaac ttttcgccgc gacgtttgcg cacccgccat
    13381 aatccgcaga agatcgcctc ctgtatttca ctttcgctgt cgtcagcctg ctgaatgcgc
    13441 actgacactt ccccttcccc gagaagcgta ttgataaagt gacgatcgtc ggcgttgaga
    13501 tttaacagat cgactgccag cacctcgccc aacggcggct ggcgttgact tagttgatgt
    13561 tgtaactcgt ttaacagcgc aatcacctgc gggctgtgag cacattgctc cagggccgcc
    13621 atactcggtt catcattcac ctgacagctg atcggcagtg gattcatgct gaaactgtca
    13681 tcgttcggtt gcgttcctgg ccccagcaga tggaaaaaag tttcgctcat gaggcacgct
    13741 cctgttgtgg ttcgacaagc ccacgcatca ggtttatcag ttccgctcac gggtgaatac
    13801 cattcagcac accgcgatag tttccgccgg taaacactaa tgtggcggga aagcgaaaga
    13861 cgccaaagcg atctccgatg gcttcgctct gttcaaggtc agcaatcgcc acctgccatg
    13921 tgtagccggg aaactcgcgc agtaattcgc caatcattac cggattatcg ctgacctctg
    13981 gcgtgcgttt cgggtcactg cttaataaca ccacgccgtc tggcgcttgc gtaagccagt
    14041 cgtcaagacg gaattcacta accggcgtcc agccgcgcgc cagcattcgt tgccacaacg
    14101 catcaaatgg cgtgtcgttg ctcatccctg ctcctccagt gtcatcctgt actggcgcaa
    14161 gcgaacgcct tcgtaatttt ccatcgacag acagtcataa ttgagacagc gcgattcatt
    14221 agccggttgc ggcacaatac cccatgcagc cagctgcgcc agcgccgcct gttccgctgc
    14281 gggcagttgc tcccgtgcca gttcgctcag gcggccgccg tagtcgtcga gcattgcggg
    14341 ttgcagaccg acgagggcaa tatgtgctgg cagatgtccg cggatatctg ccagcgccag
    14401 cacttcggag aaactgttct ggtgcaggct cattttcttc gcgctgagat aagccggaat
    14461 acgttctccg gcataggttc gcagcgttcc aggttccagc ccgtagtcaa tggcatcgag
    14521 aatcaacaga tggctggcgc tttcgacata ccccagcaag ttcagtccct gagtaccgcc
    14581 atcgacaatc tccacatact cgggccagtg gtaatgggca tacaaccgtt ccgccacccg
    14641 cacgccgaaa ccttcatcgg cccacagcag gttgcccagc cccattacca caacgcgttg
    14701 ctcgctcatg aacgctcctt gttacttatt ttgccaaatt tgtggctacg gtagccgttg
    14761 accatggtgg agatcaccgt gtcgtcggac atgatgtctt cacgcagcgc catatagaca
    14821 tgaccgatca caaacgcgcc aatcagccac atccccagcc gatgccagct gtgaatgtcc
    14881 attgagttgc ctcccgtcca gtagaaaaat tccaccacat aacggaacgg cgcaaaaata
    14941 gcgtactggc tgtgttcgct gtacagcgca aagccggtga tgatcataaa gaccgacatc
    15001 aggaaatagc cgaacatcgc cgcctgggcg atggggttat ggcctatatc ggcactcgga
    15061 cgttttgcca gaacagatac cagcggattt cataccacac gccctgccac cagcttttac
    15121 gccataccgg cacgataaac agctcgcggg agtatcgatt gccaacaaaa gcccagtaga
    15181 tccgcatcag caagaccacg gtaaaaacca tcccggcgct gaagtgaatt aacctgatgt
    15241 agcccatata gaacagatac gtcgcctcgc cgctgacgga aggtagcggc ttgccgataa
    15301 agtatccggt gaccatcaac accgccatgc ataacaccgt cagccagtgc cagatgcgga
    15361 ctggcgcttc aaagacatag tggctgacaa cgttgtcgct tttctgttgc atgatgattc
    15421 tccttcgctg ttaacgcacc tgcacggaga tcagctcgct accgtcgtcg cccagcacgt
    15481 gtgttgaaca ggcgaggcac gggtcaaagc tgtgcagagt acgcaggatc tccagcggtt
    15541 gctcggggat cgccattttg gtgttcatca gcgccgcttc ataagcgcca atctgccctt
    15601 tgggatcgcg cgggctggcg ttccaggtgg tcggcaccac gcactggtag agatcaatct
    15661 tgccatcgcg aatggcggcc cagtggccta acgccccgcg cggcgcttcg gtaaagccga
    15721 cgccacggca ctctgtcggc caggttgcag gttcccattt ttccgtggag gcggtggcaa
    15781 gattgccgtt tttcaggtta gtcatcagct tgttgaagaa atattgcaac ttacctgcgg
    15841 cccactgtgc ttcgtgcgcg cggcacaaaa tgcggcccaa cgttgactgg ataccggaaa
    15901 gcggcaggtt cagcgccgac atcatgcgat cgaccgactc aacggtcgca gcatcgcctt
    15961 tgtgataagc gattaacgtg cgtgccagcg gccccacttc catcgcgtta ccgcgccagc
    16021 gcggcgcttt gatccacgag tagcgttcct gttcattcag ctgctgaatg ttggtatcgc
    16081 tgcctttgac atcgccgggg ttgtaccacg ggtcggtaat gccatcgaat ggatgacgcc
    16141 cgacctgatc gttgggataa cgataccagg cgtggtcgac aaactcctgc acctgctgcg
    16201 gatcaaccaa atccactggc agcacattgt tgaagtcgcc gttaatcacc gcgccgccag
    16261 gcatcagcag acttttctca ccaaagtcgt tggcaatatc cgggaacgca ccgtagctga
    16321 ggacacattt atcagaaaga cccgtgccga tttcgctcca cggtttgttg aactgaccga
    16381 tggctaaggc gtcggggatc atcacgttgt taatgaagtc cgccgtgcgg gtgataatcg
    16441 actgcaccag gttcaggcgt tccatattga ccgccccgac cgcgccgctt tcgtcaatgt
    16501 taatggcgca aggcatcccg ccgacaatcc agtttggatg cgggttttta ccgccaaaga
    16561 ccgcgtggat tttgacaatt tcacgctgga aatcgagagc ttcgagatag tgggcaaagc
    16621 ccatcaggtt agcttctggc ggcagtttgt actgcgggtg cccccagtag ccattgcgga
    16681 agatccccaa ctgcccgcct tcaacaaatt ttttcaggcg gttttgtacg tcgaagaaat
    16741 agccagggga tgatttcggc caagaggaga gactttgcgc cagttcggag gttttccgcg
    16801 ggtcggcttt cagcgcatct aacacatcga tccagtccat cccggcaagc tgatagaagt
    16861 gcaccagatg atcgtggcac cagagcgttg ccagcataat gttgcggatg atattggcgt
    16921 tgtccggcac tttaataccg atagcatctt cgatggcgta aaccgaagcc agggcgtgta
    16981 cgccagtaca gacgccgcag atacgttcaa cgaacgccca cgcatcgcgc gggtcgcgcc
    17041 cttgtaggat gatctccagc ccgcgaaaca tggtgccgca ggagacggca ttggtgatca
    17101 cattctgatc gttaatattc acttcgcagc gcatgtggcc ttcgatgcgc gtaatcgggt
    17161 cgaccaccag gcggcgtccg gcattattga tggtgtatcc ctgagtttcg tactgagtgc
    17221 tcatgcctgt ttatcctcat tgcctggctg atgttcggtt tctgtaggtt gctggttatg
    17281 acgtctgcgc tggtcaacgg cgctggcgac tgcgtgcaca ccaacagccg ctgccaccac
    17341 accaagcgcg gttaaaccga cggtatcggc ggtggaatga gtacccattt gcggaatatc
    17401 gaccacgcgg ctgtagaacg aaccgcaatc ccagaaacca ttttccgcac agcccaggca
    17461 gccgtgacca gactggattg ggaaagaaac gccatcattc cagcgtgtgg aggaacaggc
    17521 gttataggtg gtaggccctt tgcagcccat tttgtacagg cagtaacctt tgcgggcagc
    17581 gtcatcatcc caactctgga cgaactctcc ggcgtcgaag tgggcgcggc gatagcattt
    17641 atcgtggatt cgctgaccat agaacatcag cggacggccc attctgtcga catctggcaa
    17701 gcgatcaaag gtcaccatgt aagtaatgat ggcgctcatc acatccggga tcggcgggca
    17761 gccaggtact ttgataatgg gtttgtcggt gatgactttg tcgataggcg ttgcctgcgt
    17821 cggattgggc cacgcggcct gcacgcagcc ccaggacgcg caggttcccc aggcgataat
    17881 cgcgctggct ccggcagcgg cacgtttgag tttctcaata aacggtcgac cgctgctgat
    17941 acagaacatc ccctgctcgc ccagcggcgg attaccttct actgcgagga tatatttgcc
    18001 attgtattgc gtgatgatgt cttcgaagac ttcttccgcc tgggttccgg cggcagccat
    18061 caaagtatcg tcataatcga gggaaatcag ggaaaggatg acgtccttcg ccagtgggtg
    18121 agcggagcgg ataaaagatt cggtacagca ggtgcattcc agaccgtgga tccataccac
    18181 cggaatgcgg ggtttgttct ccagcgccca ggcaatcttt ggtgccattc ccgcgcctaa
    18241 tcccagcgac gtggcagcca gactacaata tttgagaaag ctgcgccggg taacgccctg
    18301 acgccgcatg gcctggtaaa atgtttcctc gttattcata tcgcacgtct cttctccttg
    18361 cgacaccggc aggacaccgg gcgcttatgg tcggcaagcg acgtccctgt agcaaggcaa
    18421 accatcgctt cacgtgagat gctgaaaacg aaagctcatc cttctgcact ggcgcacgtc
    18481 gccagaaagt attgttaata aagcgtagtg aaa
//
LOCUS       DAZOZY010000070        18444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZOZY010000070 DAZOZY010000000
VERSION     DAZOZY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.070"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(6..275)
                     /locus_tag="ACHURQ_002096"
     CDS             complement(6..275)
                     /locus_tag="ACHURQ_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0823872.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            332..1000
                     /locus_tag="ACHURQ_002097"
     CDS             332..1000
                     /locus_tag="ACHURQ_002097"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0823873.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTRVQGTVVMFSA
                     SGKK"
     gene            complement(945..1082)
                     /locus_tag="ACHURQ_002098"
     CDS             complement(945..1082)
                     /locus_tag="ACHURQ_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR0823874.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1055..1153)
                     /locus_tag="ACHURQ_002099"
                     /pseudo
     CDS             complement(1055..1153)
                     /locus_tag="ACHURQ_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(1142..1381)
                     /locus_tag="ACHURQ_002100"
                     /pseudo
     CDS             complement(1142..1381)
                     /locus_tag="ACHURQ_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1480..2370
                     /locus_tag="ACHURQ_002101"
                     /pseudo
     CDS             1480..2370
                     /locus_tag="ACHURQ_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2373..2906
                     /locus_tag="ACHURQ_002102"
     CDS             2373..2906
                     /locus_tag="ACHURQ_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0823875.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2935..3462)
                     /locus_tag="ACHURQ_002103"
     CDS             complement(2935..3462)
                     /locus_tag="ACHURQ_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0823876.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(3464..6220)
                     /locus_tag="ACHURQ_002104"
     CDS             complement(3464..6220)
                     /locus_tag="ACHURQ_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0823877.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAVIEYLGLQET
                     VNKAGNAVQRSGDKMTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
     gene            complement(6372..6971)
                     /locus_tag="ACHURQ_002105"
     CDS             complement(6372..6971)
                     /locus_tag="ACHURQ_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0823878.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(7039..10490)
                     /locus_tag="ACHURQ_002106"
                     /pseudo
     CDS             complement(7039..10490)
                     /locus_tag="ACHURQ_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515772.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(10834..11514)
                     /locus_tag="ACHURQ_002107"
     CDS             complement(10834..11514)
                     /locus_tag="ACHURQ_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0823879.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(11412..12155)
                     /locus_tag="ACHURQ_002108"
     CDS             complement(11412..12155)
                     /locus_tag="ACHURQ_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0823880.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            complement(12166..12864)
                     /locus_tag="ACHURQ_002109"
     CDS             complement(12166..12864)
                     /locus_tag="ACHURQ_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0823881.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(12864..13193)
                     /locus_tag="ACHURQ_002110"
     CDS             complement(12864..13193)
                     /locus_tag="ACHURQ_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0823882.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(13190..15763)
                     /locus_tag="ACHURQ_002111"
     CDS             complement(13190..15763)
                     /locus_tag="ACHURQ_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0823883.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(15744..16157)
                     /locus_tag="ACHURQ_002112"
     CDS             complement(15744..16157)
                     /locus_tag="ACHURQ_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0823884.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(16184..16615)
                     /locus_tag="ACHURQ_002113"
     CDS             complement(16184..16615)
                     /locus_tag="ACHURQ_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0823885.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(16629..17381)
                     /locus_tag="ACHURQ_002114"
     CDS             complement(16629..17381)
                     /locus_tag="ACHURQ_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0823886.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(17389..17784)
                     /locus_tag="ACHURQ_002115"
     CDS             complement(17389..17784)
                     /locus_tag="ACHURQ_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0823887.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(17781..18356)
                     /locus_tag="ACHURQ_002116"
     CDS             complement(17781..18356)
                     /locus_tag="ACHURQ_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0823888.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(18371..>18444)
                     /locus_tag="ACHURQ_002117"
     CDS             complement(18371..>18444)
                     /locus_tag="ACHURQ_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0823889.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         4057 a         5392 c         4416 g         4579 t
ORIGIN      
        1 aatgattact ccctgacagg atttgcaggc cactcaatat caggtgcagt tgatgtatca
       61 acacgattca acaatacccg atatttattc catgcctcca gcaacgatct ttcttcctcc
      121 gttgcgattt ccagatctac agcatcctgc agtggcgcaa tatactcact aaattcctgg
      181 atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat cgaagcatac
      241 caggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat tcatcgccat
      301 aattgattta attcacaaat aaaactataa catggtgaaa tcaatgaaaa caaacacaga
      361 tgatggggct aaaatttaca caccacttac cctaaagctt tatgactggt gggttttggg
      421 agtatcaaat cggcttgcat ggggatgtcc tacaaaggaa caccttcttc cacactttct
      481 ggaacattta ggtaacaacc atctggatat tggtgttgga actgggtttt accttactca
      541 cgtacctgag agtagtctga tatctttaat ggatttgaac gaagctagcc tgaacgcggc
      601 atctacaagg gctggggaat caaaaattaa acataaaatt agccatgatg tttttgaacc
      661 ttatcccgcg gcgttacatg gtcaatttga ttccatttcc atgttttacc ttcttcactg
      721 cctgcctgga aatatatcta caaaaagctg tgtaatacgc aatgcggcgc aggccttaac
      781 tgacgatgga actctatacg gagccacaat tcttggcgat ggagttgtgc acaatagctt
      841 cggtcaaaaa ctgatgcgca tttacaatca gaaaggcatc ttttcaaaca caaaagattc
      901 cgaagaaggc ttaacacata tactctcaga gcatttcgag aatgttaaaa ccagggttca
      961 aggtactgta gtaatgtttt ccgcttcagg gaaaaaatag catccaaccg cagcacgttc
     1021 ttgcttaaga cgtgctgcgg cataatccca atgattactc cctgacaggg ttcgtaggcc
     1081 actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga tactttttct
     1141 aggcttccac caccagcacg acaagatgcc gcatacagtg acccagtcag tccagttttc
     1201 agacaaccag tgcgtcacct ttttgaaggc gctttaaagc acgttttaat ccaggtcggc
     1261 ctgtccttgt tccgcttaat ttatcttcaa atatttgttc atatcctgca caaacaagag
     1321 cgtttcgttg caggtctgta ttctggtcat ttgttgatac ccttacatag ccaatcagca
     1381 cgctgaatct cccgtccaaa agcacaaatc atgccatgca ggccagaaac cgccattatc
     1441 taaaacctcg gtttacgaga actcggcaca agcggggaga aaataccgtt actcagtaca
     1501 gcgaatacct ggactaatcg acaaacattc agcggtggcc tttctggtga actatccggc
     1561 aatgcttcta cagctgcaaa attaaaaact gccaggaaaa taagcaatgt ggcttttgat
     1621 ggttcctccg atatcacatt aaaagcaagt catgttggtg cgtttgcctt agggaaaaca
     1681 ggaagcaccg ttgcgaatga taaagcagtt ggatggaact ggagtagcgg agcctataac
     1741 gcaactatta gtggtgcatc aacgttaatt attcattttt atatgggaga aggaagttgt
     1801 cctgcagctc agtttcggat taattataaa aatggcggta ttttttatcg ttcagcccgt
     1861 gatggttatg gttttgaagc cgactggtcc gaattttaca ccaccagaaa accttcagca
     1921 ggagatgttg gtgcactgcc gttatctggt ggtcaactga atggtgcact gggtatcgga
     1981 acatccagtg ctcttggcgg taattcgatt gtattgggtg ataatgacac gggctttaaa
     2041 caaaaaggcg atggtaatct ggatgtttat gctaataacg tccatgttat gcgctttgtc
     2101 tccggaagca ttcaaagtaa taaaaccata aatattacgg gacgtgttaa tccctcggat
     2161 tacggtaact ttgattcccg ctatgtgaga gatgtcagac ttggcacacg agttgtttag
     2221 accatgaaga aaggcgtgat gtatgaaaaa tcaggccatg taatcacggg gcttggtatt
     2281 atcggtgaag tcgatggtga tgatccggca gtattcagac caatacaaaa gttaattaac
     2341 ggaacatggt ataacgtatc gcaggtataa tcatgcagca tttaaaaaac attgtcgcag
     2401 gtaatccaaa aaccgttgaa caataccagc taacaaagaa ttttgatgtt atctggttat
     2461 ggtccgaaga tggaaaaaat tggcatgagg aattaaaaaa ctttcaggag gatacaataa
     2521 aactggctta cacagttgag ggaataatta ttgctataga caaggatgta tcagcaatta
     2581 atccagaagg tttaagtgtc gttgagttgc ctgatattac agcaaatcgc cgggctgata
     2641 tttcgggcaa ctggatgttc aaagatggtg cggtgataaa gcgaacttat accgaggaag
     2701 agcaaaggca acaagcggaa aatgaaaagc agagcctgct gcagctcgtc agggataaaa
     2761 cccagctgtg ggactctcag ctacagctgg gcatcatttc cgatgagaat aaacaaaaat
     2821 taaccgagtg gatgctctat gcgcagaagg ttgaatccac agacacctcc agcctgccag
     2881 taacgtttcc cgaacaacca gaatgagaga aggcccgcta tcgggcctta atttttattc
     2941 aggcttttgt ggccattcag gatttgccgt atccacacgg ctgaccagaa cactgtagcg
     3001 ttcccatgct tccagtcgtg tgcgttcctc gtctgttgcc atattcagcc tgacagcgcg
     3061 ttccagcggc tggatgactg attcagcttc ggaaagcaat gcggcctttt gtgattcggc
     3121 ctgttgttgc agttcctgtt ctgtatattc cctctttact actttaccat ccagaaacat
     3181 ccattttccg ttattgtctg cgtgacgatt tgccgtaata tcaggaactt caactatgct
     3241 tagcccttca ggattaatag ttgaaacgtc tttactgaca gcaacgatga tgttgttctc
     3301 atcgtaagca atttttatgg tgtctggctg aaaattcttt tgttcctcat accagttttt
     3361 gccatcttcg caaaagagcc ataccacgcc cgctttttta gttagctggt attgttccgg
     3421 tgttttcgga ttcccggcct taatattttt caggtgcatc atcttaaaca ctccccacgt
     3481 tgtaccatat tcccccaatc aatttctgaa ttggccttct ggcaacagtg tcgacaatat
     3541 catccgcatt gctgttaatt gcggctgtta aaacataacc tgacgcatcg ccaaagccgt
     3601 agtcgcgcca tacgttttta tattcaatgc tgccaagacg aatatcccga acgacatgat
     3661 tatgcagcca tgtgcttaac cagttgtttt cccacactga accataaata tcgccactag
     3721 acgcaacttg cgcaccactt cccagcgcaa atcccccatc tgttttaaaa atgaaatcac
     3781 caccaccatc tgaaccgtta ttaatatgaa caccatcacc accgttatct ttccacaaaa
     3841 acatacgaat tcttccgtct tcgttggcga atgtaatgtg gtggcggtaa ttacttttta
     3901 atctaaaatt gctgccatta gattcaatat ctttataaag atgtaatcct aactcatcaa
     3961 tatgtccgat gcgcgaacca tttgaataga agctaataat accatctcca tcttgcctaa
     4021 atccggtgtc gttatctccc agaacaatgg agttttcacc cagtgcgtta tcagtagctc
     4081 caatagctag acctccttta attttggccc cgtggctgac agatatagca cctgttctca
     4141 gatttatagc gaatggcctt aatgggccga tattaccgtt ttcgccttgt ccttcagccg
     4201 tcggaatgaa atgaagaaaa tcttctgaac gacggaaaat tagaccgaag gtatcattga
     4261 aaattcgcag cgcattcacc gtgccaattt tcagttctcc ggtcatttta tcgccggaac
     4321 gctgaacggc gttaccagcc ttgtttaccg tttcctgtaa accgaggtat tcgataacgg
     4381 cagcaacggt cgatttcgca agaatatccc gcccgacttt tgtcagggtt gccaggctgg
     4441 cgacatcatt ccccgtaaaa tacggaaact tgcctgccgc agtagtaagt ccggccagcg
     4501 ccgtcagggt ggcatctttc ggttgcttac ccgcaagcgc gttagtcatg gtggtcgcaa
     4561 aattcgggtc gttgcccagc gccgcagcca gctcgttcag cgtgttcagt gcatcaggtg
     4621 acgaatctac aagggcggca atcgcagcca taacgaaagc cgtgcttgcg atttgggtat
     4681 tattcgttcc ctgtcgcgca gttggtgttg ttggcgttcc ggtcagtgca ggactattta
     4741 agggcgcttt cttgttcgtt tcacccatta ccgccttaac cgcttttggc gttgccgcca
     4801 gtgactcgga agtgctgttg gtcgcactgc tgagctgtac tatccccttt ttcgtcgtgc
     4861 tcgcatcctc cagcgccacg gcggatgcaa tatcctctgc ccgttttgct gctgtctcgg
     4921 cgcgcgttgc cgcggattca gcagcaactt tgctctgaga tgcagccgtc gcactgcctg
     4981 ccgcctctga tgctttcgtt gttgctgtcg tggcactacc tttcgctgct gacgcttgtc
     5041 tggtcgcctc atcttttgaa gcagacgcag atgatgccga tgacgccgct gaactggctg
     5101 acgatgcggc tgctgcctta gaggaagcag cattgtctgc tgaagtcttt gcatttgttt
     5161 cagaggtttt tgctgcagaa gcagacctcg ctgctgcagt ggcttgctca gtggctttgc
     5221 cagccttcgt tgtggctgtt gaagcggatg atgcggcgct ttctgccgat tttccggcgg
     5281 cggtggcact ggctgaggcc tgcccggcac ttgttgacgc ggcactggca gataatgcag
     5341 ccgctgtttt tgaacctgcc gcagctgagg cgctctgtcc cgctgctgtt tcagaagact
     5401 tagcgttcgt ctcggacgtt tttgccgcct tcgcggaatt tcctgccgcc gttgccgagg
     5461 aagctgcact actggcactt gatgatgcat tcgtttctga agatttcgct gcctcttttg
     5521 aggccgccgc accccgtgcc gaggtggcag cttctgacgc cttcgtggtc gctgtggatg
     5581 cagaagtggc tgccgatttt tgtgatgctg cggcattcgt ttctgacgtt ttcgcggcac
     5641 tggcgctggt agctgccgcg ctttttgatg actctgcagc agcacttttt gctgcttcac
     5701 gggcctttgt cgatgccgtt cctgcgctgg aagacgctga ctgagccgac gacgcggcct
     5761 gtccggctga cgtgctggcg gcacgtgctg aggctgcagc atcggttgca tgagttgccg
     5821 cctcgctggc tgattttttc gcggctgccg tattctgtgc aaccgcggag gcgttacgtg
     5881 acacctcttc caccatctgc tcaaagcggc gcagtgcctc cggtcggaca tcatcctccg
     5941 tcatggcacc gagaaaatca ttcagcgtac ctggtctgga accttcatag acggtaatgg
     6001 tcccggcatg tgaaggcgga aaaccttcaa ccagcagggt gacgctgtac tgaccatgct
     6061 caacatccat gctgtaacgt ccggcttcat ccggattttc agaggccacc gtgttcacca
     6121 ccatcgtgct gctggttcgt ctggccttca gcacaatggt gcagttctgt actggttttc
     6181 ctgtgccatc tttaagcacg ccagaaattt ttactgtcat acttttccac caataaaaaa
     6241 agcccgcagc agtgacgcca cgggcttcag gacagtgtaa ctttacgttt cctcaaacgc
     6301 agttcacccc ataaggtgga tgaacctgcg tatcataaca atatttacag aagataaatc
     6361 ggcgtctgtt gtcagatacg gtatccgata ccaacaataa atgcatccgt tcgccagtca
     6421 ccactaccgg aaccttcata agcaaggtca atggtcacgg attcggtcgg gttaaactgc
     6481 acgccagccc cccacgccag agacgtgttg ctgtggcgac cgtcatcact tccggtcagc
     6541 acatcgtgcg ttttcccctt gttgtcagtt acgcggagat aatccccgga gaaagtcgac
     6601 acacggctgt aagccacacc caccatcgca tacgcgctga accattcatt cacgcgtaca
     6661 gacggccccg ccatcacgct gaaccagcgg ttacgcacgg aatcttcatg ccagcgggta
     6721 tcgctgtagt gcgttttttg ctcatcctca gcattggcat aactgaagga cgtaatcagc
     6781 cccagcgcgt ccgtaaactc ataacggtat ttcacgttaa tcccgttcag attatcgctg
     6841 ccgggagcgt tcgtacgggc atgaagatac cccgcgctca gtgtggactg atgttcagac
     6901 gcccatgcag gcgcaccgga tacggacaga cagatggctg cggacaaaat ggctgcacaa
     6961 actttacgca taattacctc tcgcttttct gcaataaaaa aggcgccatt tctggcgccc
     7021 gtatatgggt tataaaattc agctgatact gatgcctgcg gtggctttct tcatcaccac
     7081 aaccagcaaa tcgctgatac ttgctgtggg ataccagtta ttcaccagcc atgctgacac
     7141 cgaaaactcc agcgtcatgt ggccgcgacc agctggcata tcaataacac cactgtaaat
     7201 cagcgtatta tccagcgcgg tacggttata aatttcagca ccgtttttcc gtactatcag
     7261 gcggcatgac gaataaatat cgttattctc ccgctcatgt ttagcgccgc tgaatgccac
     7321 cgccggaata acaatttgcc tgtcaaaagg ctgatcgtca taaatcctga cggtaatggt
     7381 ccctgatggc caccgctccg gtgcccggga gtcccgcgga aaagccttac ccactgtttt
     7441 gactatatcg ccttcaatct ggttggctga cagtttcccc ttaatctgac agttctcatt
     7501 aattgtgaca ttgttgagcg tcccggcgtt cgcattcaca ctgccactga tatctgcatt
     7561 tttagcggtc agttttccgt ccggtgtcag ggaaaatgcc ggaggattac cgccgctggt
     7621 aatggtggga gccgtcaggc gtttcaggaa cacgtcgttc atgaatatct gatcgccctg
     7681 accaacaaac atcggctttg tgttgccatt cgcaggatta atcatcgcaa tcctgtctgc
     7741 cgccagcagc acctgactct gcattcctgc tggcgtattc tcaataccgg caccgatacc
     7801 cgcaatataa aggcgtccgt ccttcatctg ttgcagcttc acagcccaca tgctgttcag
     7861 attattattt gtatcaacct gaaccttctg tatctgctgg attgccgcac tctggtcttt
     7921 cagtttctta ttgacagtct gcgtgatttc attgctgaca tccgtaatgg acgtcctgat
     7981 ttcagccagg tcaggcgcaa gctgaccgtt atcaatctgc gtccacagct cctgagccag
     8041 atgtgttttc cctatctctc ctttgaaaaa atccaagtaa ccttccgcat catcgctcgc
     8101 ccgaccgaca gcctccacga atgccgattt gccaacggtg ttcacactgc gaacgtaaaa
     8161 ataataatca tggcccggtt tgatattgat actggcggct atccagtaca gcgccgtgcc
     8221 aagatagcgg gcgctggttt caacctgcct gatatccgca atccgctttt ccgagaacca
     8281 gaactcaaac tgtaccgtcg ggtcataaac ggcaagatgc ggcgttgcgg ttatctgaaa
     8341 atagcccggc gtcagctcaa tccgcgacgg tgctgccggt gcggcaatcc ggaaggtggt
     8401 ggtggcaggt tcaccctgct ggccatagct gtttatcgcc cgcaccgtca gggtgtattc
     8461 cccgagcggc aggccgctga aacggtgctc cgtgtctgcg gtgatggcgg tggtcaccag
     8521 tctggcatcc gttcccttac cactggtcag gcgcagactg aagcgcacac ccttcaccac
     8581 ccgcggcgtg tcccatttcg cctgcgccag atactggccg tcaggtgctg cactgccggt
     8641 gggatgacgc tgttcaggga acctgactgc ggctcaaagc gggcaccgtt atccacgatg
     8701 gcttcttttt ccagtacgtg ctgcaccgcc gtgatggcaa aggtgccgtc cgtgttttcc
     8761 cggacggaga cacagcggaa caggagacgg cgcagtgacg gcagggagag tccccacacc
     8821 ccgtatgtct ccacaccatc aggcagggta ctgacctgta tccggtccgg cgcggggtgt
     8881 tcggtgatgt ccacactcac cggcttaccg ctgccgttaa tcaggttcac cgtggcggca
     8941 ccggtctccg gaagtgtcac ttcacggtcc agcgtcagag tgcgggtggc agcatcaatg
     9001 gacaggacac gtccgccggt cagggtcccg gcatagtcgt tatcacagat ttcaatgatg
     9061 tcaccgggtg tgtgccgcag cccctgagac ccgagcgtga aatccaccgt ctgcgtttcc
     9121 agcagttcgg tctttatcac ccacagtccg gcacggtggg cctgaccgcg gctggtacag
     9181 ccgaacgcgt ccatcttcag caggttgcgt ccgtagcgca gtatggcttc cgggtcttcc
     9241 accagttccg tggaggtctg ccagccgttc tgcgggtcgg tataattcac ctccaccgcc
     9301 gtgtgccggt ccttcagggc actgaagctg tagcggaaac ccacgccgtt atcatccacc
     9361 accacatcgc tgttggtgta cggccacacc acatccgacg ggcggtcctg aacaaacgtc
     9421 agcgtctggc cgttccatac cggcatacag cgcatcgccg agcagaaatc actgagcacg
     9481 tcccatgcct tacgctgttg tgccaggtac gcattgaagg tcatccgcgg ctcggtctcc
     9541 ccgaaactat ccgggaccgt ctggtcgcag tactgcccga tggcatacag cgcccacttg
     9601 tccacatccg ccgcccccag acgttttccc atgccgtagc gcgggtgagt cagcatgtcc
     9661 cacagacacc aggccggatt gttgctgtat gccggtttca ggctaccgtc ccagatacca
     9721 ctgtacgtgc gtttttccgg gtcatagttt gacggcacct ggatgatgcg accgcggata
     9781 tggtagttca ccgtcatctg ctggccgccg aactgctccg catccacctg cagccccaca
     9841 atggccgtgt tcgggtagca ctgtttcaca tcgatgattt cggtgtatga cgaccacagc
     9901 gtcttattct gcagctggtc cgtggtgctg tccgctgtct cccggaccat ccggatgtta
     9961 aaaggacggg gaggcagatt atccagaatc accgacgcca gaaactgcga ggtggtcttg
    10021 ccgttaatgg tgacatcctt ttctgtcacc cagttaccgt tacgctgtaa ctgaatcagc
    10081 aggcggacgg atgtcgggtt tcggtcaccc tttgacgtgg tctgcaccag tgactgcacc
    10141 ccgaaggtaa cccgcaggcg gtcaatgttc gcggacgtaa tggtgcgcgt caccggtttt
    10201 gccttcgtca cttccacgcc cagtccggtt tcagcaccgg aggactcaaa gccttccggt
    10261 ggtgtctgct cctgctcccc ggcgcgtcag accgccgtca caccgtgtat cacgggatta
    10321 ccgtccgtgt ccgtcagcgg ggttttgttc accaggatac tctgcagtcc cttcaccgga
    10381 ccttctatca gtccctcacc aatcgcatca atcacgctca tcatctgcgt ggatttgaga
    10441 ttatccttcg cctcacgagg cgtgtgtgcc ttaccgccac cttttcccat acagccttcc
    10501 cctgaataaa ttaaccgcca cttgccattc cgtacagaag tcggatatcc ttcgcccgaa
    10561 aagcatgaaa cacatttctg ccatgctaaa gagaaacccc ggtatcagca gataccgggg
    10621 ttttctttca tgcccaccga taatcctgtt ggttaaaacc ggtaatggca taaaaattct
    10681 gaatatcttc acattttcac aaactgactg tggcgcgtat aatttctctg cgttaatttt
    10741 tttgtcgtga tataagaata attccttaca cttaatcttc gtaactctcc cgcagttcct
    10801 gtccgcgatc actgcgggat ttttttattc tttttacccc tgccgcccga taaccacgac
    10861 ctttccgccc ccgccttcat cacgggtgct gatgtcctgg gatatacggc gggagccaac
    10921 cagcatttcc ccgtaaggca ccggcatcgg gttcccctgg gcaatcatgt tatccagtga
    10981 ggaaaagtac gtgttctgtc tgccgttatc cgttgcgcgg taatccggtg tttttgcctt
    11041 cggggccagc atctgggcca caccgcccag aatcatgctg gctccaagtg aaaacagcat
    11101 cgtggtggca gaaaaaccac cggctgccag ggctgaaccc cataacgcca ttgatgcccc
    11161 ggcagtgaag aaagagccca cgatggctgc cgcccccagc acaatctgca gtccaccctt
    11221 tccggccccg gccagtcgcg gcacaatgtg gatgaccgtt ccctcaccca gctgttcgtg
    11281 aagacgggcg tacaccgcct ccggtgccgt gtcataaccg gcaatacgta tctggtacca
    11341 gccttcgttc atctgacggc gaaagcccgg catctgcatc gacagggcgc gaatggcttc
    11401 cgctgccgtg ttcacataca ggctgaggcg gcggccaaat cgttgtaaat ccccgtgaag
    11461 gcagatgcgt gccagtggcg gtgacgccag actgaatgcg ttcgtcgttg ccatttttcg
    11521 gaatacctct cccgtttact cagttgttca ggcagatggt gaagcagctc accgttgccg
    11581 cagtaaatgg cggcatggtt cggtaccgaa gcaccaaagc agcacagcag aatatcgccc
    11641 gcctgtgcag aggacagggg cacccggtaa aagccggtga ccgccatatt gtccaggtaa
    11701 aggttctgac cgttgcgcca ccagtcatcc tcgcgatgaa aatccggcat ttcagtcccc
    11761 gccagatgat aagcatcccg gaacagcgtg taacagtccg tcaccccgtg ctcaaagcgc
    11821 cgtcctgtca gatgtggcac acagcggaat ttgtgaatgt caccccggca gaccaaccac
    11881 cagggcagtg cgctttttat ctgcaaccgc cggtcagcct cgctcagcca gggcagccca
    11941 ccgggatgac tgtggaccag tgccacaatc tccccctgca tctctgcccg cagccagtct
    12001 tccggtgcga tacgaaaata cgcctccggc tctgcggaaa tattcacaca agggatatac
    12061 cgctccccct ccggcgtgct tatcacgaag ccgcacgact ccgcaggcgc acaccgccgg
    12121 gcatgtgcca gaatcgctga ttcagtctgt gtcataaacc gggatttact gcgaaagttt
    12181 attaatggaa aggaaaccgc caaaattgcc gacattcctg cgcagttcac acccgcgcat
    12241 gcacttgctg catctgtcct tacggatatc cgtggtgggg ttgtcgaact catccgccac
    12301 cgcaggaccg ttatacccgc attcatctcc ccggtaatcc cacatacagg tgttcgccag
    12361 catgatgcga ccgggaaaca gcgccccgtc cgtctcggtc ggtgtagcca gcacaaacga
    12421 ggccgtcatg gctgtcagct gcgacatctg ctccaccacc cagcggtcac tcagctcctg
    12481 ctccgggtcc gcctccggat tgcccgcaac gaaattcacc gcatccagaa aacgggcata
    12541 cacccggcgg cggaccaccg tggcccccac cagactctgc aggtcttccg ccatcccggt
    12601 gaccagaccg aacagattgg acaccgtcag cgacggtctg gcactgctgc cccggccgtt
    12661 catctcaaag ccgctgccgt caatcgggta tgcctcatac ttacgcccct gccaggtgac
    12721 cggctcccct ttttcattca gctcattaca gaaaaaatac cgctcaccac cctgtaccgt
    12781 caggtcgatt tcccagagta ccacccgcgg tgactgctct gacttaaccg actcgttcag
    12841 actttcttcg cgaatatcct gcatcagttc accacctgct taaactccgc gctgaactca
    12901 acgcgcaaca tcccgacccg cgcagaccac ccggcacagg tcacctttat ctgccggtat
    12961 gcatagggtg gcttccacaa aaatgccttc cagccaccgt gctctgccag gaacgcttcc
    13021 agatgccggg cctcctcccg ggtcacggaa agcgtcaccc tgtatgtttt caggtcagca
    13081 ttcagccctg ccgccatacg ctgtgagtac ccgtcaccaa aacgcacttc acgcaccgat
    13141 ggctgcgagt tcacctccat atccggcttc actttccagc gaaaggtttt catccaccgc
    13201 tccctgataa cataccgcca tcacgcaact gcagccggag ttcatcctgt gcccccttgc
    13261 gggccatctc atacaccgct ttcatcagct gcggccctgc ccgcccgttg gggccgtcgt
    13321 tctgaatcac cacgtgattg ttctgattaa aattaatgcc ttccgcccgc cgcatctgcg
    13381 ccggacttcc ggcaccgccg acataaccac cttccgcata gccccgcatc aggcggtaca
    13441 gattgccgac accaatccgg ctggtcgcct ccttcgtgaa gacaaactcc ccgcgatgaa
    13501 caatccccgc aggttcatat ttaccccccg tccccgtaaa tcccccggtc gcgaaatgga
    13561 agttcgccgc cgcagcctga atggccgtcc ccgtggaggc agacgcacca ccaccgaaag
    13621 caccgccaat ggcgctgccg atactcccga ctatccccac catcgcctgc ttcagaaaaa
    13681 tctctgtcag catggacagc acagaacggg tgaaaccacg ccagttctgt tcgctgccga
    13741 tcagcatcgc tgccatattc tgtgcaatac cgtcaaaggt ctgcgtggcc gcgtttttaa
    13801 cctgcgaaaa actgtccgtc gcactttccg cccactcgcc ccagccggac ttcatcccgg
    13861 ccatccagct tccacgaagc tgctcctccg cagaccaggt gttcttcagt gcagatgtgg
    13921 ccttcgccag cgcagccgga ttatcaccgt acacctcacg aaggcgctgc tcttccgact
    13981 cccgctgcgc ctgacggtcg gtgagtccgc gggcttttgc gctgattgcc gcctgcttcg
    14041 cgctctgctg ctgttcaaac cgcgccgcct gctgtgccag ctcattcagc cgtttctggt
    14101 gttcaatctt gtcgcccagc tcagccagct ggcgtttgta ctccagcgtt tctttctcat
    14161 gggccagcag ggatttttcc tgctcagata actgccgttt cgtggcggcc tctttcagga
    14221 ccacatactg attttccgct ttccataaat cacggcgctg ctggctgatt ttttcattcg
    14281 caccgctgtg cttctccagc gtcctgagct cagtttcaag cgccagcagg gctgcatgcg
    14341 cctggtcttc ctggcgctca ccggcagaca ccttcacacc ggacggcttt ttctgcgtcg
    14401 actcataatc cttttttgcc gacgccatca gcgtgttgta atccgcctgc aggattttcc
    14461 cgtctttcag ggccttattc agctcttcct gccgggcggt atatttctcc agtggcgtca
    14521 gcaggcgctc atacgccttc tgcgcctctc cggtatactt cagctgtgac gactcacgct
    14581 cagccctgtc ccttgccgcc agttcaccgg ctttttccat atccgactgc agcgttgccg
    14641 ctgccagacc cagacgggca ttttcccggt catcccatgc gccctgaagg ttggcccgga
    14701 aagaggaggt tttaccgcgg cgctggctcc gactctggta ccactgccat tttttatccg
    14761 cctcatcaaa tgccttctgc gcactggcga gcatatccgc tgaggattcc ggacgaccga
    14821 tatccagaat ggcatcccac atcgatttga atgccttccc tgttttatcc gcccaggtct
    14881 ccagtgttcc catgttttct ttcaggcgac gggtctgctc atcaaagcct ttcgtggcga
    14941 tatcgttcgc cgcctgcaat gccccggcct cgtctccgga acgctgcagc tgtgcaacat
    15001 acgcaatctg ctctgccgtc acgttacgga actggcgcgc catcgccatc agtcccgacg
    15061 tcgggtcagt ggtcagcttc ccgaaggctt cagcgacttt atccacctcc acaccggatg
    15121 cagacgcaaa acgcgcgaca ctctggttga tggcatcaaa ctgttcacca ccacgcacac
    15181 cggcattcac cagggctgcc agtgactctc tcgcctggtt aaacgtcagc cctgctgcct
    15241 gcccggctct tgagagagtc agcatacgat cggcagtcag tccggactga ttaccggaaa
    15301 gaaccagggt tttattaaac gctgaaagcg tggaatctcc ctggtaccag gcgtacacca
    15361 gtgcacctgt cgccaccgcc agcgaggtga ccccgaccat cggcagggtg atcgcatcgg
    15421 caagccccct gaacatgggg atcatcccgc cgaaggagtc cttcacctga ccgccctgtt
    15481 gcagcaggat cagccaggga ttctgaccac cggcaagctg cgtggcgata tccgtaaact
    15541 gtgcgggcag ggttcgcatg gccgctttat actgcccgac ggaaatcccg gctttttgtg
    15601 cagccagcgc ctggcggctc aggccctgtt caacagcact ggcggttttt ctggcgtcgg
    15661 tatccagacc tgaaaaatga cgccttaccc ggctcatctg ctcatcgaaa cggacagcat
    15721 ccagactcag gtcaataaca agatcaccaa ccggctggga catatctcac acctcccgga
    15781 atccccgctg aagccatcat taatgcggca tcatccacca tgacatccgc cacatccgca
    15841 gacgataaaa tatcgcgccc tccgtcccca ccgaaccgga cgcctccggc aagtcctgcc
    15901 gctttctgca tcagcatttt gtcctcatcc ggcctctcca cctgctcttc ctcatgccgg
    15961 gggacaagca gactgaaatc agagggatgc atatccggat cgcaaaaaaa caggctgagt
    16021 acagcgtacg tcagcccgga aaaatgcata tccagctggg tatcctgaaa ataatgcgtg
    16081 cggtaaaaac ggcgccagtc ggcatattcg gtggatgtca tcccggcaag catggcgcgc
    16141 cagtcgggtc tccccatctc acgcgccagt ctgagggcaa agttcagctc gccgtcgaag
    16201 actttcccgc agaaaaatca tcatcagtca gcgtgttatt tttcgccact tcagtaatat
    16261 cagtatccgg acgaacagct tcgatcatcc cggacaggca caacaccacg tcttccgccc
    16321 gggcaatggc atcggcaggc caggtggtga gcacttcctg ctctatcttc atcacggcct
    16381 cattcattga cggtgactgc gttttctgtg gatggttatg ccacagggac atcgccacca
    16441 gaaacgcgcc ggttctgacg agatcttcca cgcttacctg caggttgccg ctggattctg
    16501 cctgttctgc acgccgtttc aggagggcaa gatgctcgat acgctgcagc gcagacaatt
    16561 cggaaagcgt gacagacaca ccgttatatt caaattgttc tgttttcaga aacatgtatt
    16621 acctccgttt accctgcagc gcccgcttca gtaacggtga cttcagccac tgcggcgaac
    16681 tgaccatttc cgctcaccac agggatctgc accttacctg tcgccacgcc gtttaccgta
    16741 attgtcatat ctttcacact aatggtggct ttcgacggat cggcggaaac cgctctgaac
    16801 gtcttgtcgg ttgcactttc cggctcaaaa gaaaccgtca gggtggttgt tttccctttt
    16861 gccaccgtac cggatgtcgg cgtcacctta atcgcactga ccggcgtaat tttgctgcgt
    16921 tcttccgcta cagaaggttt acccacgtta gtgactttca ccgtgcgggt gatcacttct
    16981 ttcgccgtca cggccttacc gatactgctg acccagccac gaaacacatc caccgtgcca
    17041 ttcggaaaac ggattttata ggcccggaca tcgccgcttt caaaccagcc tataagccct
    17101 ttctggcctt cctctcccgg tttccaggcc agcgtaaaac tggtatcacc tgcagatttc
    17161 tgcccctgcc cggtcgcgct ccagtccgcg tcttcatcat ccaggtagtt atcatcatag
    17221 gattcagccg tcatctcgcc cggcgtcaga tccttcacct tagccagtcg ctgccagtca
    17281 tcgtctgaca acgggtttgc ataagcatca gccttgccgt tgtaaaccca cagagtggta
    17341 ccggcacctt ttaccggctc caggggattt ggtgttgcca tatcgtcctc acatctcgta
    17401 tgtaatggaa taagtcagat ccgcagagct ccataacgcc atatcgtcat cacgacgata
    17461 ctcatagccc tgcgtaacca tcgtggtaat cagtcctgcc agtgcaggga tcgcagtcat
    17521 cgccggataa atccggcttt ccatccactg atcgagctcc gaatcaggta cctgtgccgg
    17581 taaaaacacc tcaatatgca gtgtggcccg ccaggtatct gcatccagct cttcaccggt
    17641 atactctgca tccgtcagat aaaccgcgat cgcaggaaaa tcctcttcgt caaaaacaac
    17701 ggggcgacca tcaaacagcg tcgccccgtg ttcatgctgc tcgagtgcat ccagcactgc
    17761 ggcacgaatg tcagtgtgtt tcatcgtttt atcgcaatcc tcagttgttg tttcagcgcg
    17821 tatgccagtt ctttaggcag gcgttcacgc cggatacggt caacattctc atcaaatgcc
    17881 tgtttcagtg gggccgccat cgggattttc accacctgaa tgggaaggcg attacgcttt
    17941 ttccttccct tgtcgtcatt gccctcctca tatctggcct ggggaagacg ttgcataaca
    18001 tgccagcgcc cattatttaa tcgctggata aatgcccgct gataacgatg ctgaccggct
    18061 ttgagtatgc tgttcggacg acgccccagc attctgatcc ccagcttaat cacagggaga
    18121 tcaccgcggt taacgataat tctggcattc ggatttctga ccgtcgcccg tttcagtctg
    18181 gaccgttcct ttaccagttt ccgtctcacc ctggtttccc gggcaacctg tgacgaagac
    18241 tgattaatcg ccgttgtggc cacgcggtta atggtcattg cagaagcagc cggaatggcg
    18301 tttttacgaa cccggttcag attgtcaatc gcctgatcaa gcccttttat cgccataatt
    18361 caccctgcgt ttatcgtcgc cggttaactg cgggtggttg cccacggttg agccagagat
    18421 aacagctgcc cccgtcatcc ggag
//
LOCUS       DAZOZY010000071        18316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZOZY010000071 DAZOZY010000000
VERSION     DAZOZY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.071"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..369
                     /locus_tag="ACHURQ_002118"
     CDS             <1..369
                     /locus_tag="ACHURQ_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR0823890.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
     gene            complement(403..813)
                     /locus_tag="ACHURQ_002119"
     CDS             complement(403..813)
                     /locus_tag="ACHURQ_002119"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823891.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            960..1811
                     /gene="hchA"
                     /locus_tag="ACHURQ_002120"
     CDS             960..1811
                     /gene="hchA"
                     /locus_tag="ACHURQ_002120"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR0823892.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHURQ_002121"
     CDS             complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHURQ_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR0823893.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHURQ_002122"
     CDS             complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHURQ_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR0823894.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHURQ_002123"
     CDS             4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHURQ_002123"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR0823895.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4603..5607
                     /gene="msrP"
                     /locus_tag="ACHURQ_002124"
     CDS             4603..5607
                     /gene="msrP"
                     /locus_tag="ACHURQ_002124"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR0823896.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHURQ_002125"
     CDS             5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHURQ_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR0823897.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            6500..7150
                     /gene="zinT"
                     /locus_tag="ACHURQ_002126"
     CDS             6500..7150
                     /gene="zinT"
                     /locus_tag="ACHURQ_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR0823898.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7493..8023
                     /locus_tag="ACHURQ_002127"
                     /pseudo
     CDS             7493..8023
                     /locus_tag="ACHURQ_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(8593..8682)
                     /locus_tag="ACHURQ_002128"
     tRNA            complement(8593..8682)
                     /locus_tag="ACHURQ_002128"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:Ser,seq:cga)
     gene            8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHURQ_002129"
     CDS             8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHURQ_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR0823899.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9674..9749
                     /locus_tag="ACHURQ_002130"
     tRNA            9674..9749
                     /locus_tag="ACHURQ_002130"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9707..9709,aa:Asn,seq:gtt)
     gene            10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHURQ_002131"
                     /pseudo
     CDS             10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHURQ_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(17390..>18316)
                     /locus_tag="ACHURQ_002132"
     CDS             complement(17390..>18316)
                     /locus_tag="ACHURQ_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR0823900.1"
                     /translation="AIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTM
                     TMQDHATLKEIKDLAKNITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGIT
                     MNCYSPLCKNASRMDQKLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEM
                     RSLGSYIAFNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDC
                     YNNVYRLSLPPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAM
                     ADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         5188 a         4316 c         4063 g         4749 t
ORIGIN      
        1 gcaatgatgt atgctgaaac ccgcaatatg acaccatatg gtaatgtggg tatcgcaaat
       61 aaaacccaga actttgaagc tgtagcacag tatcagttcg attttggtct gcgcccatct
      121 cttgcatatg tttattcaaa aggcaaagac ctcggcggta acgactataa caacaatggt
      181 catcaagagt atgttgatca ggatctggtc aactatgttg aaattggagc gacctactat
      241 ttcaataaga acttctcaac atatgttgac tataaaataa acttgttgga taaagatgac
      301 gatttctatg acaacaatgg catcgcaaca gatgacgttg ttggtgtggg cttagtctac
      361 cagttctaat cagccaacgt gctgcggcgt gttactgtaa aactaaacac tagcctgagt
      421 tcgtccctca tcgaattcag gcttttttat tggcatttca tctccatctg cggcacacgc
      481 aattgccaat gcatatcgat acagcacctt accctgcccc tcctggcaca ataacatcaa
      541 ccagcgactc ttcaaaccaa cacccaaatg catttcttta tgaattaatc cgtaacaaac
      601 acgcttccca tatagaaata tttgcaaaaa tagtgcatga aataatcttt tctctaccaa
      661 tccgcgatat aaatattcat cagtaccaca atatttcaca gcactaaatc tctccccgcc
      721 gccccgtacc tttgataatg gtctaaaatc attgaggcca cttgcgacga cgttgctcaa
      781 taaggaaaat ttggcgtgaa taatattcaa caccagtgca gcatcatcat tacatactta
      841 cacccgccga ctaccgcttt cgatatattg actcaatttt ccagactggt taaaaatagc
      901 gaccagctca gtcgcaaata tagtgactac cctaactaag caacaataag gaatacacta
      961 tgactgttca aacaagtaaa aatccgcagg tcgatattgc tgaagataat gcattcttcc
     1021 cttcagaata ttcgcttagc caatatacca gtcccgtctc tgatcttgat ggcgtggact
     1081 atccaaaacc gtatcgcggt aagcataaaa ttctggtgat cgccgcggac gaacgttatt
     1141 tgccaaccga taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc
     1201 cgttgtatca tctccatgct gctggtttcg aattcgaagt ggcgaccatt tccggtctga
     1261 tgaccaagtt tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg
     1321 agcagcataa atcgttgttc cgcaatccga agaaactcgc ggatgttgtc gccagcctca
     1381 acgctgatag cgaatatgca gcaatctttg ttcctggtgg tcatggcgca cttattggtt
     1441 taccagaaag ccaggacgta gctgctgctt tgcagtgggc aatcaaaaat gaccgttttg
     1501 ttatctccct ttgccacggc ccggcggctt ttctggcgct tcgccacggc gataacccac
     1561 tgaatggtta ttccatttgc gcattcccag acgccgcaga caaacaaacg ccagagatag
     1621 gctatatgcc gggtcatctc acctggtact tcggcgaaga gctgaagaaa atgggcatga
     1681 acattattaa tgacgacatc accgggcgag tacataagga ccgtaaagtt ctcaccggcg
     1741 acagtccttt cgcagcgaat gcgttgggta aactggcggc gcaggaaatg ctggcagctt
     1801 atgcggatta atccctgtta cgtttgaatg taaacgcata atcaatcgca gccacgttaa
     1861 tactcacgac gtggctgcgt tttttcatgg ttgatttatg gcgtactgaa gccctatgtt
     1921 aatttctttg cggtaacata atccggaaca cattatgctt attgagatag tgatacgtag
     1981 cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt ccctgaccta
     2041 cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca ggctcatgaa
     2101 ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca agaaaactgg
     2161 ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata agattcgata
     2221 acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg accttaaagc
     2281 aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg acttccttat
     2341 tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg gcaagaaaca
     2401 gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg gcaattgttt
     2461 tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga cccagtaatg
     2521 cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt agacgctcaa
     2581 aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt tttagttctc
     2641 tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc gcttccgtta
     2701 caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga cttaatactg
     2761 agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac tgttcaagca
     2821 tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc gaaacttcat
     2881 caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg taaatgccag
     2941 ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg acatttgtcc
     3001 ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga ctaacatcca
     3061 tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt aacaactgct
     3121 tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac tcacttgcca
     3181 ggccattata gagagtccag acaattccag cgccagcaac agacagcagc aatataaaaa
     3241 gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg ctacgaatga
     3301 atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat caacttttgc
     3361 gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt tgattcccca
     3421 aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg ccagtagcca
     3481 aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc tcacactttg
     3541 acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag cgtgatgttg
     3601 ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata
     3661 atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc ttgcagtaag
     3721 gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc agccatccat
     3781 acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg caagataaag
     3841 cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa gcccctgcgt
     3901 tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga aaataatatg
     3961 ccataaatca tatgttatag aaacagccta gttcattaca aaattgtaat gctgctgtaa
     4021 ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa ggatataaac
     4081 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
     4141 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
     4201 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
     4261 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
     4321 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
     4381 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
     4441 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
     4501 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
     4561 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
     4621 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
     4681 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
     4741 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
     4801 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
     4861 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
     4921 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
     4981 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
     5041 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     5101 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     5161 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     5221 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     5281 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     5341 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     5401 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     5461 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     5521 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     5581 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     5641 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     5701 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     5761 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     5821 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     5881 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     5941 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     6001 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     6061 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     6121 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     6181 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     6241 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     6301 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     6361 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     6421 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     6481 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     6541 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     6601 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     6661 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     6721 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     6781 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     6841 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     6901 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     6961 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     7021 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     7081 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     7141 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     7201 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     7261 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     7321 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     7381 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     7441 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     7501 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     7561 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     7621 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     7681 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     7741 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggggat
     7801 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     7861 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     7921 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     7981 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     8041 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     8101 tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt aagacttatc
     8161 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     8221 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     8281 taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct acgcaacttc
     8341 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     8401 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     8461 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     8521 tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc aggagataag
     8581 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     8641 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     8701 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     8761 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     8821 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac ggaacaggaa
     8881 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     8941 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     9001 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     9061 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     9121 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     9181 gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca taagctggac
     9241 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     9301 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     9361 gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg ttttgccgta
     9421 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     9481 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     9541 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     9601 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgtgcc
     9661 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     9721 cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt tagcgcaatg
     9781 tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac accttaagcg
     9841 ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt ttcccctcga
     9901 ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca aaactgccta
     9961 acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt tcgctgtgct
    10021 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
    10081 agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa actacgccgc
    10141 ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc ggcagcacaa
    10201 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat tgcaaatgcc
    10261 aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
    10321 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
    10381 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
    10441 accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag tacttcacag
    10501 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa tatggcgcgt
    10561 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
    10621 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
    10681 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
    10741 actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
    10801 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
    10861 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
    10921 accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc ggccaatggc
    10981 tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg taaactggtc
    11041 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
    11101 cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac cttcagcgcg
    11161 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
    11221 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
    11281 cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct ggaatatcgc
    11341 aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc aggagaagtg
    11401 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
    11461 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
    11521 ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
    11581 accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt ggtcgttcag
    11641 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
    11701 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
    11761 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgactgg
    11821 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
    11881 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
    11941 atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa ggaccgttat
    12001 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
    12061 aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa accaggagtc
    12121 actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac cgcctatacc
    12181 aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg ctggatttat
    12241 catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca ataatggtgt
    12301 actcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa
    12361 cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa cttccttcaa
    12421 caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc tgaaaagtag
    12481 taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat
    12541 cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt cgaaaaatga
    12601 agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg atgcaaaagg
    12661 caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg cgaaactgag
    12721 ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca gtttgaaaaa
    12781 tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc aacaggtgaa
    12841 ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag gtgatatcac
    12901 cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa atggcaaccc
    12961 actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta agttctcgat
    13021 tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct ccctgaccgg
    13081 cacgttagcg ggcacgcata tgatcatggc tcgtcgggct aacagcaatg tcagcgatgc
    13141 acagccaatg acgtttgtgg cggataaaga cagagcggtt gtcgttttgc aaacatcgaa
    13201 agcggaaatc attgggaatg gcgtggatga gacaactctg acagcaacag tgaaagatcc
    13261 gtcgaatcat ccggtggcgg ggataacggt aaacttcacc atgccacagg acgttgcggc
    13321 aaactttacc cttgaaaata acggtattgc catcactcag gccaatgggg aagcgcatgt
    13381 cacgctgaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgctgg gtaataacaa
    13441 taccagtgat tcgcagccgg taacatttgt ggcggacaaa gcctcggctc aggttgtcct
    13501 gcagatatca aaagatgaga tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac
    13561 ggttaaagat cagttcgaca atgaggtgaa taatcttccg gtaacattca gctcagcctc
    13621 ttcaggactc accctgaccc cgggagtaag taataccaat gagtctggca tcgcgcaggc
    13681 cactctcgca ggcgttgcct ttggtgagca gacggtcact gcatcactgg ctaataatgg
    13741 tgccagcgac aacaaaactg tgcattttat tggcgacaca gcggcggcaa aaattatcga
    13801 gttgacgcct gtcccagaca gcataatcgc cggtaccccg cagaacagct ccggcagcgc
    13861 catcaccgcc acagtcgttg ataataatgg ctttccggtg aaaggtgtga ctgtgaactt
    13921 caccagcaga acaaactctg ccgaaatgac gaatggcggc caagccgtaa cgaacgaaca
    13981 gggtaaggct accgtcactt ataccaatac ccgctcctcg atagaatcag gagcgagacc
    14041 ggataccgtt gaggccagtc tggaaaatgg tagctccacg cttagcacat caattaatgt
    14101 caacgctgat gcgtctacgg cacatctcac ctggctacag gcactttttg atacagtctc
    14161 cgcaggcgag acaaccagtc tgtatattga ggtgaaggat aattacggca acggtgtccc
    14221 ccagcatcag gtgaccctca gcgtttcacc aagtgaaggc gtgaccccca gtaataacgc
    14281 tatatatact accaaccacg acggcaattt ttacgcaagc tttaccgcta caaaagccgg
    14341 ggtttatcaa gtgacggcaa ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta
    14401 tgtgccgaac gtcacgaatg ccgaaatcac gctggcaacc tcgaaggatc cggtgattgc
    14461 cgacaataac gatctcacga cactaacagc aacagtcgct gatacagagg gcaatgcgat
    14521 agccaacact gaagtaacat ttactctgcc ggaagatgtt aaggcgaact tcacgctgag
    14581 cgatggcggt aaagcgatta ctgatgctga aggcaaagcg aaagtcacgc tgaaaggtac
    14641 aaaagcaggc gctcatactg ttacagcatc gataactggc ggtaagagtg agcagttggt
    14701 ggtgaacttt actgcggata cgctcactgc gcaggttaat cttaacgtta ccgaggacaa
    14761 ttttatcgct aataacgtcg ggatgaccag gctgcaggca acagtgactg atggaaacgg
    14821 caacccgtta gccaatgagg cggtgacatt cacgctaccg gcagatgtga gcgcaagctt
    14881 tactctcgga caaggcggtt ccgccattac tgacatcaac ggcaaggctg aagttacact
    14941 gagcggtaca aaatccggca cctaccccgt gacagttagc gtgaacaatt atggtgtcag
    15001 tgatacgaaa caggtgactt tgattgccga tgctggtacc gcaacactag cctccttaac
    15061 ctctgtatac tcattcgtcg tcagcacgac cgagggcgcg accatgactg caagcgtcac
    15121 tgacgctaac ggcaacccgg tagaaggcat aaaagttaat ttccgcggaa cctccgtcac
    15181 gctaagcagc accagcgttg aaacggatga tcggggtttc actgaaattc ttgtgacaag
    15241 caccgaagtc ggactgaaaa cagtttcagc ctctctggca gataaaccta ctgaagtcat
    15301 atcgcgatta ctgaatgcaa aagcagatat taattctgca acgattacca gtctggagat
    15361 acctgaaggt caggtcatgg tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca
    15421 gtttggcaac ccgattctta atgaatctgt aacattcagt gcagagccac cagagcacat
    15481 gaccatcagc caaaatattg tctctactga tacgcatggt atagccgagg tctccatgac
    15541 gcccgaaaga aacggttcgt atatggtgaa agcatccctg gcgaatggag cctcacttga
    15601 gaaacaactg gaggctattg atgaaaaact gacactctcg gcgtccagcc cgcttatcgg
    15661 tgtcaattcc ccaacaggtg caactctgac ggcaacgcta acttctgcaa atggcactcc
    15721 agtggagggt caggtcatca actttagcgt aacgccagaa ggtgcgacgt taagtggcgg
    15781 aaaagtgaga actaactctt ctggtcaggc tccagtcgtt ctgaccagca ataaagtcgg
    15841 tacatatacg gtgactgcat cgttccataa cggcgtaaca atacagacac agacaaccgt
    15901 gaaagtcact ggcaactcaa gcaccgccca tgttactagc tttatcgctg atccatcgac
    15961 tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg
    16021 taacctgatc gaaggtctca ctgtgtactt tgccttaaaa agcggctctg ccacattaac
    16081 gtcattaaca gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggagcgat
    16141 gacaggtagc gtcacggtaa gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat
    16201 aacgctggtg gcaggcccag cagacgcctc aaagtcagtc cttaagaaca atcggtcatc
    16261 attgaaagga gactttaccg atagtgctga gctacatctt gttctgcacg atatatcagg
    16321 caatccgatc aaagtttctg aagggctgga atttgtacag tcaggtacca acgtgcccta
    16381 tgtgcaagtt agtgcaattg actacagtaa aaatttctca ggcgagtaca aagccactgt
    16441 tacaggaggc ggagagggta tcacaacgct gatccctgta ttgaatggtg ttcatcaagc
    16501 tggtctgagt accacaatac aattcactcg cgcagaagac aaaataatga gcggtacagt
    16561 atcagtcaat ggtactgacc taccgacaac tacattccct tcgcaggggt tcaccggggc
    16621 gtattatcag ttgaataatg acaactttgc cccaggaaaa acggcggctg attatgagtt
    16681 ttcaagctct gcctcctggg tcgatgttga tgctaccggt aaagtgacat ttaaaaatgt
    16741 cggcagcaat tcggaaagga ttacggcgac accaaaatca ggaggcccta gctatgtata
    16801 cgaaatccgt gtgaagagtt ggtgggtgaa cgccggcgag gctttcatga tatacagcct
    16861 tgctgaaaat ttttgcagca gcaatggcta cacgctcccc agagcaaact atttaaacca
    16921 cagtagttcc cgaggcatcg ggtcactgta cagtgaatgg ggagatatgg ggcattacac
    16981 gactgaagct ggttttcaat caaatatgta ttggtcatct agtcccgcaa actcaagcga
    17041 acaatacgta gtttccctgg caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc
    17101 ttatgcgaca tgttataaaa acctctgatt ttccgttata atttcttaaa gagtgctcca
    17161 ttcggagctc tcttttttac attccctcta tagagcttgc ccttctctca cagaaaatga
    17221 ataaataaaa atgcggcacc gccagaattg cgttcgatgt gactatgatc gtatccggtg
    17281 agtactgctt tgattcaaag gctggttttc tgtgatgatt gaccagcctc agagtattga
    17341 tgacaccacc gttcataaca gatcggcatt actatgcata agtactgtct catattctcg
    17401 ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc
    17461 caacgacgac tgactccgag acatcggcca ttgcgggcca tcgcgttttg taatcagcat
    17521 aaattgcaac aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag
    17581 ttattgcgat ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtggtaagg
    17641 ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct tcacctttgg
    17701 gtccatgaac gatcactatt ggtatatctg tttcactttg aatttttgct attagatttt
    17761 ctgcaatcga taatgaaaat gtacgttcct gcgagctacc ttctaaattg aacgcaatgt
    17821 aagatcctaa cgatcgcatt tcctcgcgca cctcatcgag tacatcctca cttagtggca
    17881 attcatatat tggcctgact gctggaaaac ccgcctcacg catcataaat gcccatgtca
    17941 taggtacggg agcccggagt ttctgatcca tacgggacgc gttcttgcac aaaggggagt
    18001 agcaattcat ggttatacca acaacctgaa aattcgtttt tgctttcaac tgactgataa
    18061 ataacatcgt tttcaggttc tttttacgca tcccctcaat acaaagatcc ggcgtaccgt
    18121 attgctgtgt tatgttcttt gctaaatctt ttatttcttt taatgttgcg tgatcctgca
    18181 tagtcattgt gactaatgtt aatttagtct gttcaagttt aagcgcatta aagacttcta
    18241 aattaattgt cgacgttaca attaaaagat gcttaatttt atgcaattca agcgcccgaa
    18301 taacaggaaa gatggc
//
LOCUS       DAZOZY010000072        18213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZOZY010000072 DAZOZY010000000
VERSION     DAZOZY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.072"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHURQ_002133"
     CDS             15..1091
                     /locus_tag="ACHURQ_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0823901.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHURQ_002134"
     CDS             complement(1484..1825)
                     /locus_tag="ACHURQ_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR0823902.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHURQ_002135"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHURQ_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR0823903.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHURQ_002136"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHURQ_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR0823904.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHURQ_002137"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHURQ_002137"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR0823905.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHURQ_002138"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHURQ_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR0823906.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHURQ_002139"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHURQ_002139"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR0823907.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHURQ_002140"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHURQ_002140"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR0823908.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHURQ_002141"
     CDS             complement(7167..7826)
                     /locus_tag="ACHURQ_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR0823909.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHURQ_002142"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHURQ_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR0823910.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHURQ_002143"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHURQ_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR0823911.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHURQ_002144"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHURQ_002144"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR0823912.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHURQ_002145"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHURQ_002145"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR0823913.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHURQ_002146"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHURQ_002146"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR0823914.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHURQ_002147"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHURQ_002147"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR0823915.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHURQ_002148"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHURQ_002148"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR0823916.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHURQ_002149"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHURQ_002149"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR0823917.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHURQ_002150"
     CDS             complement(17270..18112)
                     /locus_tag="ACHURQ_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR0823918.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4670 a         4574 c         4393 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaa
//
LOCUS       DAZOZY010000073        17956 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZOZY010000073 DAZOZY010000000
VERSION     DAZOZY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.073"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(227..1201)
                     /gene="cysB"
                     /locus_tag="ACHURQ_002151"
     CDS             complement(227..1201)
                     /gene="cysB"
                     /locus_tag="ACHURQ_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR0823919.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1411..4008)
                     /gene="topA"
                     /locus_tag="ACHURQ_002152"
     CDS             complement(1411..4008)
                     /gene="topA"
                     /locus_tag="ACHURQ_002152"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR0823920.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4388..4639
                     /locus_tag="ACHURQ_002153"
     CDS             4388..4639
                     /locus_tag="ACHURQ_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR0823921.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="ACHURQ_002154"
     CDS             complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="ACHURQ_002154"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR0823922.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5944..6702
                     /locus_tag="ACHURQ_002155"
     CDS             5944..6702
                     /locus_tag="ACHURQ_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR0823923.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6699..7289
                     /gene="cobO"
                     /locus_tag="ACHURQ_002156"
     CDS             6699..7289
                     /gene="cobO"
                     /locus_tag="ACHURQ_002156"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR0823924.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="ACHURQ_002157"
     CDS             complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="ACHURQ_002157"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR0823925.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8414..9528)
                     /locus_tag="ACHURQ_002158"
                     /pseudo
     CDS             complement(8414..9528)
                     /locus_tag="ACHURQ_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9556..10176)
                     /locus_tag="ACHURQ_002159"
     CDS             complement(9556..10176)
                     /locus_tag="ACHURQ_002159"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR0823926.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10173..11054)
                     /gene="rnm"
                     /locus_tag="ACHURQ_002160"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(10173..11054)
                     /gene="rnm"
                     /locus_tag="ACHURQ_002160"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR0823927.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11192..11236
                     /gene="trpL"
                     /locus_tag="ACHURQ_002161"
     CDS             11192..11236
                     /gene="trpL"
                     /locus_tag="ACHURQ_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR0823928.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11213..11306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11328..12890
                     /gene="trpE"
                     /locus_tag="ACHURQ_002162"
     CDS             11328..12890
                     /gene="trpE"
                     /locus_tag="ACHURQ_002162"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR0823929.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12890..14485
                     /gene="trpD"
                     /locus_tag="ACHURQ_002163"
     CDS             12890..14485
                     /gene="trpD"
                     /locus_tag="ACHURQ_002163"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR0823930.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14486..15847
                     /gene="trpCF"
                     /locus_tag="ACHURQ_002164"
     CDS             14486..15847
                     /gene="trpCF"
                     /locus_tag="ACHURQ_002164"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR0823931.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15859..17052
                     /gene="trpB"
                     /locus_tag="ACHURQ_002165"
     CDS             15859..17052
                     /gene="trpB"
                     /locus_tag="ACHURQ_002165"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR0823932.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17052..17858
                     /gene="trpA"
                     /locus_tag="ACHURQ_002166"
     CDS             17052..17858
                     /gene="trpA"
                     /locus_tag="ACHURQ_002166"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR0823933.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4317 a         4749 c         4488 g         4402 t
ORIGIN      
        1 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
       61 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
      121 aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa gagaatatat
      181 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
      241 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      301 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      361 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      421 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      481 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      541 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      601 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      661 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      721 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa tcagcattgc
      781 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      841 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      901 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      961 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
     1021 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
     1081 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
     1141 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1201 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1261 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1321 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1381 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1441 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1501 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1561 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1621 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1681 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1741 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1801 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1861 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1921 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1981 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     2041 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     2101 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     2161 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2221 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2281 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2341 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2401 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2461 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2521 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2581 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2641 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2701 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2761 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2821 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2881 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2941 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     3001 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     3061 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     3121 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3181 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3241 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3301 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3361 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3421 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3481 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3541 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3601 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3661 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3721 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3781 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3841 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3901 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3961 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     4021 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     4081 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     4141 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4201 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4261 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4321 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4381 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4441 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4501 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4561 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4621 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4681 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4741 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4801 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4861 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4921 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4981 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     5041 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     5101 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     5161 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5221 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5281 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5341 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5401 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5461 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5521 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5581 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5641 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5701 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5761 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5821 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5881 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5941 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     6001 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     6061 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     6121 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6181 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6241 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6301 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6361 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg tcagggacga
     6421 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6481 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6541 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6601 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6661 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6721 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6781 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6841 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6901 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6961 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     7021 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     7081 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     7141 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7201 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7261 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7321 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7381 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7441 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7501 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7561 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7621 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7681 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7741 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7801 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7861 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7921 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7981 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     8041 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     8101 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     8161 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8221 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8281 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8341 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8401 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8461 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8521 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8581 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8641 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8701 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8761 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8821 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8881 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8941 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     9001 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     9061 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     9121 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9181 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9241 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccatcag gctgcatcca
     9301 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9361 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9421 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9481 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9541 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9601 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9661 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9721 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9781 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9841 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9901 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9961 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    10021 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    10081 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
    10141 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10201 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10261 agttcgatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10321 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10381 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10441 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10501 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10561 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10621 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10681 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10741 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10801 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10861 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10921 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10981 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    11041 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    11101 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    11161 tacgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11221 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11281 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11341 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11401 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11461 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11521 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11581 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11641 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11701 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11761 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11821 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11881 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11941 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    12001 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    12061 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    12121 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12181 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12241 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12301 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12361 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12421 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12481 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12541 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12601 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12661 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12721 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12781 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12841 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12901 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12961 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    13021 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    13081 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    13141 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13201 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13261 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13321 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13381 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13441 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13501 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13561 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13621 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13681 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13741 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gcggcctgtg ggctgaaagt
    13801 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13861 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13921 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13981 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    14041 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    14101 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    14161 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14221 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14281 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14341 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14401 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14461 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14521 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14581 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14641 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14701 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14761 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14821 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14881 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14941 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    15001 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    15061 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    15121 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15181 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15241 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15301 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15361 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15421 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15481 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15541 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15601 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15661 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15721 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15781 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15841 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15901 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15961 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    16021 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    16081 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    16141 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16201 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16261 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16321 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16381 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16441 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16501 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16561 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16621 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16681 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16741 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16801 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16861 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16921 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16981 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    17041 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    17101 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    17161 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17221 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17281 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17341 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17401 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17461 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17521 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17581 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17641 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17701 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17761 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17821 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc ccccagccgc cagttccgct
    17881 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17941 ctttttaccg tttcgc
//
LOCUS       DAZOZY010000074        17870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZOZY010000074 DAZOZY010000000
VERSION     DAZOZY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.074"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            155..833
                     /locus_tag="ACHURQ_002167"
                     /pseudo
     CDS             155..833
                     /locus_tag="ACHURQ_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            877..5727
                     /locus_tag="ACHURQ_002168"
     CDS             877..5727
                     /locus_tag="ACHURQ_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFR0823934.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKTLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6095..7750
                     /gene="lldP"
                     /locus_tag="ACHURQ_002169"
     CDS             6095..7750
                     /gene="lldP"
                     /locus_tag="ACHURQ_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR0823935.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7750..8526
                     /gene="lldR"
                     /locus_tag="ACHURQ_002170"
     CDS             7750..8526
                     /gene="lldR"
                     /locus_tag="ACHURQ_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR0823936.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8523..9713
                     /gene="lldD"
                     /locus_tag="ACHURQ_002171"
     CDS             8523..9713
                     /gene="lldD"
                     /locus_tag="ACHURQ_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR0823937.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9897..10370
                     /gene="trmL"
                     /locus_tag="ACHURQ_002172"
     CDS             9897..10370
                     /gene="trmL"
                     /locus_tag="ACHURQ_002172"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR0823938.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10423..11244)
                     /gene="cysE"
                     /locus_tag="ACHURQ_002173"
     CDS             complement(10423..11244)
                     /gene="cysE"
                     /locus_tag="ACHURQ_002173"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR0823939.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11324..12343)
                     /gene="gpsA"
                     /locus_tag="ACHURQ_002174"
     CDS             complement(11324..12343)
                     /gene="gpsA"
                     /locus_tag="ACHURQ_002174"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR0823940.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12343..12810)
                     /gene="secB"
                     /locus_tag="ACHURQ_002175"
     CDS             complement(12343..12810)
                     /gene="secB"
                     /locus_tag="ACHURQ_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR0823941.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12873..13124)
                     /gene="grxC"
                     /locus_tag="ACHURQ_002176"
     CDS             complement(12873..13124)
                     /gene="grxC"
                     /locus_tag="ACHURQ_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR0823942.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13266..13697)
                     /locus_tag="ACHURQ_002177"
     CDS             complement(13266..13697)
                     /locus_tag="ACHURQ_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR0823943.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13942..15486
                     /gene="gpmM"
                     /locus_tag="ACHURQ_002178"
                     /gene_synonym="pgmI"
     CDS             13942..15486
                     /gene="gpmM"
                     /locus_tag="ACHURQ_002178"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR0823944.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15520..16779
                     /gene="envC"
                     /locus_tag="ACHURQ_002179"
     CDS             15520..16779
                     /gene="envC"
                     /locus_tag="ACHURQ_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR0823945.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16783..17742
                     /locus_tag="ACHURQ_002180"
     CDS             16783..17742
                     /locus_tag="ACHURQ_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFR0823946.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
     gene            complement(17729..>17870)
                     /locus_tag="ACHURQ_002181"
     CDS             complement(17729..>17870)
                     /locus_tag="ACHURQ_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR0823947.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4484 a         4623 c         4675 g         4088 t
ORIGIN      
        1 tgaaacgaat gatacatttt tatgatgaat gcttaaaaca atatttaatc gacacacatc
       61 actgtcatta atgtaaagca acatagatca ccaataatac aatggacaac tctaccggca
      121 ttgatcgctg gtcggaggaa ataaggattt ttacatgaac ttgaagaaga tttttttcag
      181 tgctgtcact gtttcagttc tctgcgcatt aacgggatgt gactacattg aagaaggcaa
      241 gccagaaagt agtttattga aacaacaaga agaacattaa aattgatcta cttgaaaaac
      301 aacaagcaca gctaaagagt caactggaaa ctattcaaaa acaacaaact ggcataataa
      361 gcagtacaaa gactttaact cacgtaatta aatccgttaa agatcaacaa aacactttta
      421 tttttaccga gtttaaccca gcaaaaacga agtatttcat cctgaacaat ggttcggtgg
      481 ctttagcggg tcgagtgtta tccattgacg ccacagagaa tggcagtgtt attcatattt
      541 cactggtcaa cttattaagt acacctatct caaatattgg ttttaatgcg acatggggtg
      601 gtgaaaaacc tgtcgatgcc aaagagtttg cccggtggca acaattactt ttcaacacat
      661 caatgaaatc cacattgaaa ttattaccag gtcaatggca agacattaat ttgaccctga
      721 agggtgtatc gcccaataac ctgggatatc tgaagttagc catcaacatg gaaaatattc
      781 agttcgacaa tcttccctct gctgaaaatc ggcagaaaag aagcaaaaaa taaatgcagt
      841 tagtacgctt ataacaagga atagcgagca tcattaatga acaaaatatt taaagttatc
      901 tggaaccctg cgacagggaa ttatactgtt accagcgaaa cggcaaaaag ccgtggcaag
      961 aaatctgggc gcagtaagct gttaatttct gcgctggttg cgggtggaat gttgtcgtcg
     1021 tttggggcat tggcgaatgc cgggaatgac aacggccagg gtgttgatta cggtagtgga
     1081 tcagctggcg acagctgggt tgctataggc aaaggggcga aagcaaatac ttttatgaac
     1141 accagtggtt ccagtactgc tgtgggttat gacgctatag ctgaaggcca atatagctct
     1201 gccatcgggt caaaaaccca tgcgattggt ggtgcatcaa tggcctttgg ggttagtgca
     1261 atatcagaag gcgatagaag tatagcactg ggtgcctctt cgtattcatt gggccaatac
     1321 tcaatggccc tcggccgtta ttcaaaagca ttgggtaaat tgtctattgc tatgggggac
     1381 tcttccaaag cggaaggagc aaacgccatt gccctgggaa atgccactaa agctactgag
     1441 attatgagta ttgctcttgg cgacaccgcc aatgcgtcaa aagcgtattc aatggcgctg
     1501 ggagcaagta gcgtcgcatc tgaagaaaac gcaattgccc tggggcgtag cagtgtagct
     1561 agcggtactg acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt
     1621 gcgataggtg ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag
     1681 gcaaaaggga ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact
     1741 atcgcattag gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc
     1801 aacaaagctg atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta
     1861 aacaccattg ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt
     1921 agtaatagca gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat
     1981 ttagacaata ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa
     2041 aatggcgcga tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt
     2101 agcgactcgg tagcaaaaac gcttggagga ggggctgcag tagatgttga tgacggtact
     2161 gttacagcac caacctacaa tttaaaaaat ggtagcaaaa ataacgtagg ggctgcgctc
     2221 gctgtacttg atgaaaacac cctgcaatgg gaccaaacca aaggcaaata cagcgctgct
     2281 catggtacta gtagcccaac tgccagcgta atcaccgatg ttgcggatgg cacgatttca
     2341 gcctccagta aggatgcggt taacggttcc caactgaaag ctaccaatga cgatgtcgaa
     2401 gccaacaccg ccaatatcgc tactaatacc agcaacattg ccacgaatac agcaagtatt
     2461 gccaccaata ccaccaatat caccaacctg acggattccg ttggtgacct tcaggctgat
     2521 gccctgctct ggaacgaaac taaaaaggca ttcagtgcag ctcacggcca ggataccacc
     2581 agcaaaatca ccaacgttaa agatgccgac ctgacggctg acagcactga tgctgttaac
     2641 ggctctcagc tgaaaaccac caacgatgct gtggcgacga ataccaccaa tatcgccaat
     2701 aacacttcca atattgccac taacaccacc aacatctcta atctgactga gacggtgact
     2761 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtattcac ggcagctcat
     2821 ggcaccgaga ccaccagcaa aatcaccaac gttaaagatg gcgacctgac gactggcagc
     2881 accgatgccg ttaacggctc tcagctgaaa accaccaacg atgccgtggc gacgaatacc
     2941 accaatatcg ccactaacac caccaacatc tctaatctga ctgagacggt gactaatctt
     3001 ggtgaggatg cgctgaaatg ggataaggac aatggtgtct tcactgcagc tcatggcaac
     3061 aataccgcca gcaaaatcac caatatcctg gacggcacag tcactgcaac cagttccgat
     3121 gccattaacg gtagccagct ttatgactta agcagcaata tcgccaccta cttcggcggc
     3181 aatgcttctg tgaatactga cggtgtgttt accggtccaa cctacaaaat cggtgaaaca
     3241 aattattata acgtcggcga tgcactggct gcgattaact cctcatttag cacgtctctc
     3301 ggcgatgctc tgctttggga tgccaccgca ggtaaattca gtgccaaaca cggtactaat
     3361 ggtgacgcaa gcgtgatcac tgatgtcgca gatggtgaaa tttcagactc cagttctgac
     3421 gcagtaaacg gctcacaact ccacggcgtg agcagttatg ttgttgatgc gctggggggt
     3481 ggtgccgaag tcaatgcaga cggcaccatc actgcgccga cgtacaccat tgctaatgct
     3541 gattacgata atgtcggtga tgccctgaat gctatcgata ccactcttga cgacgctctg
     3601 ctctgggatg cggacgccgg tgaaaatggt gcatttagcg ccgctcacgg aaaagataaa
     3661 actgccagtg taatcactaa cgtcgctaac ggtgcaatct ctgctgccag cagcgacgcg
     3721 attaacggct cacaactcta taccaccaat aagtacatcg ctgatgcgct gggtggtgac
     3781 gcagaagtca acgctgacgg caccatcacc gcaccgactt acaccattgc gaacgccgag
     3841 tacaacaacg tcggtgacgc cctggatgcg cttgatgata acgccctgct gtgggatgag
     3901 actgccaatg gcggtgctgg agcctacaat gccagccatg acggtaaagc cagcatcatc
     3961 actaatgtcg ctaatggcag tattagtgag gacagtaccg atgcagtgaa cggttctcag
     4021 ttgaatgcga cgaatatgat gattgagcag aacacccaaa ttatcaatca gctcgctggt
     4081 aacaccgacg caacctatat cgaagaaaat ggcgctggta tcaactacgt tcgtactaac
     4141 gacaacgatt tagcctttaa cgatgcaagc gcctctggtg tcggcgctac agctgtaggt
     4201 tataacgctg tcgcgtctgg tgccagcagc gtagccattg gtcagaacag cagcagcacc
     4261 gttgataccg gtattgcgct gggtagcagc tccgtttcca gccgtgtgat tgccaaaggt
     4321 tctcgtgaca ctagcgtaac ggaaaatggc gtggtgattg gttatgacac cactgacggc
     4381 gaactgctag gtgcattgtc aattggtgat gacggtaaat accgccaaat catcaacgta
     4441 gctgatggtt cagaagccca tgacgccgtt acggttcgcc agttgcagaa cgctattgga
     4501 gcggtcgcca ctacgccaac caagtacttt cacgccaact caacggcaga agactcactg
     4561 gccgttggtg aagactcgct ggcaatgggt gcgaaaactg tcgttaatgg taatgcaggt
     4621 attggtatcg gtttgaacac tctggttcta gctgatgcga tcaacggcat tgctatcggc
     4681 agtaacgcac gcgcaaacca tgcaaacagt atcgcaatgg gtaacggttc tcagaccacc
     4741 cgtggtgcac agactggcta caccgcctac aacatggacg caccgcagaa ctctgtaggt
     4801 gagttttctg tcggtagtga agacggtcaa cgtcagatca caaacgtcgc agctggttca
     4861 gcggataccg atgcggttaa cgtgggtcag ttgaaagtca ctgatgagcg cgtagcgcaa
     4921 aatacccaga gcattactaa cctgaacaat caggtcacta atctggatac tcgcgttact
     4981 aatatcgaaa acggtattgg cgacattgtc accaccggta gcaccaagta cttcaagacc
     5041 aacaccgatg gcgtagatgc caacgcccag ggtaaagata gcgttgctat tggttctggt
     5101 tccattgctg ccgctgacaa cagcgtcgca ctgggtaccg gttccgttgc agaggaagaa
     5161 aatacaatct ctgtaggttc ttctactaac caacgccgta tcaccaacgt agctgccggt
     5221 gttaatgcca ccgatgcggt taacgtgtcg cagctgaaat cttctgaagc aggcggcgtg
     5281 cgctacgaca ccaaagctga tggttctatc gactatagca atatcaccct cggtggtggc
     5341 aacggcagta cgactcgtat cagcaacgtc tccgctggcg tcaacaacaa cgacgcggtg
     5401 aactacgcgc agttgaagca aagcgtgcag gaaacgaagc aatacaccga tcagcggatg
     5461 gttgagatgg ataacaaact gtctaaaact gaaagcaagt tgagcggtgg tatcgcttct
     5521 gcaatggcaa tgaccggtct gccgcaggct tatacaccgg gtgccagcat ggcttctatt
     5581 ggtggcggta cttacaacgg tgaatcggca gttgctttag gtgtatcgat ggtgagcgcc
     5641 aatggtcgtt gggtctacaa attacaaggt agtaccaata gccagggtga atactccgcc
     5701 gcactcggtg ccggtattca gtggtaatga tccattaagt tagtgtgact aagggcaaac
     5761 gccaaattgc ctgatgcgtt acgcgcatca ggcaagataa ggcgccccgg gtcaacaatc
     5821 taagggcaat tctctgatga ggattgccct tttctttacc agacatctcc ccccacaaga
     5881 attggcccta ccaattcttc gcttatctga cctctggttc acaattcccc aattaaaact
     5941 cacatcaata ttgccaatac ataacattta gttaaccatt cattgtcatt atccctacac
     6001 aacacaattg gcagtgccac ttttacacaa cgtgtgacaa ggagatgagc aacagactca
     6061 ttacacgatg tgcgtggact ccaggagacc tgcaatgaat ctctggcaac aaaactacga
     6121 tcccgccggg aatatctggc tttccagtct gatagcatcg cttcccatcc tgtttttctt
     6181 ctttgcgctg attaagctca aactgaaagg atacgtcgcc gcctcatgga cggtagcaat
     6241 cgcccttgcc gtggctttgc tgttctataa aatgccggtc gctaacgcgc tggcctcggt
     6301 ggtttatggc ttcttctatg gtttgtggcc catcgcgtgg atcattattg cagcagtgtt
     6361 cgtctataag atctcggtga aaaccgggca gtttgacatc attcgctcgt ctattctttc
     6421 gataacccct gaccagcgtc tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct
     6481 tgaaggagcc gcaggctttg gcgcaccggt agcaattacc gccgcattgc tggtcggcct
     6541 gggttttaaa ccgctgtacg ccgccggact gtgcctgatt gttaacaccg cgccagtggc
     6601 atttggcgcg atgggcattc caatcctggt tgccggacaa gtaacaggca tcgacagctt
     6661 tgagattggc cagatggtgg ggcggcagct accgtttatg accattatcg tgctgttctg
     6721 gatcatggcg attatggacg gctggcgcgg tatcaaggag acgtggcctg cggtcgtggt
     6781 tgcgggcggc tcgtttgcca tcgctcagta ccttagctct aacttcattg ggccggagct
     6841 gccggacatt atctcttcgc tggtatcact gctctgcctg acgctgttcc tcaaacgctg
     6901 gcagccagtg cgcgtattcc gttttggtga tttgggggcg tcaaagattg atatgacgct
     6961 ggcccacacc ggttacactg cgggtcaggt gttacgtgcc tggacaccgt tcctgttcct
     7021 gacagctacc gtaacactgt ggagcatccc gccgtttaaa gccctgttcg catcgggtgg
     7081 tgcgctgtat gagtgggtga tcaacattcc ggtgccgtac ctcgataaac tggttgcccg
     7141 tatgccacca gtggtcagcg aggctacagc ctatgccgcc gtgtttaagt ttgactggtt
     7201 ctctgccacc ggcaccgcca ttctgtttgc tgcactgctc tcgattgtct ggctgaagat
     7261 gaaaccgtct gacgctatca gcaccttcgg cagcacgctg aaagaactgg ctctacccat
     7321 ctactccatc ggcatggtgc tggcgttcgc ctttatctcg aactattccg gactgtcatc
     7381 aacactggca ctggcactgg cgcacaccgg tcatgcattc accttcttct cgccgttcct
     7441 cggctggctg ggggtattcc tgaccgggtc ggatacctca tctaacgccc tgttcgccgc
     7501 gctgcaagcc accgcagcac aacaaattgg cgtctctgat ctgttgctgg ttgccgccaa
     7561 taccaccggt ggcgtcaccg gtaagatgat ctccccgcaa tctatcgcta tcgcctgtgc
     7621 ggcggtaggc ctggtgggca aagagtctga tttgttccgc tttactgtca aacacagcct
     7681 gatcttcacc tgtatggtgg gcgtgatcac cacgcttcag gcttatgtct taacgtggat
     7741 gattccttaa tgattgtttt acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg
     7801 gcgctgattg atgaaaaaaa cctggaagcg ggcatgaagt tgcccgctga gcgccaactg
     7861 gcgatgcaac tcggcgtatc acgtaattca ctgcgcgagg cgctggcaaa actggtgagc
     7921 gaaggcgtgc tgctcagtcg acgcggcggc gggacgttta ttcgctggcg tcatgacaca
     7981 tggtcggagc aaaacatcgt ccagccgcta aaaacgctga tggccgatga tccggattac
     8041 agtttcgata ttctggaagc ccgctacgcc attgaagcca gcaccgcatg gcatgcggca
     8101 atgcgcgcca cacctggcga caaagaaaag attcagcttt gctttgaagc aacgctaagt
     8161 gaagacccgg atatcgcctc acaagcggac gttcgttttc atctggcgat tgccgaagcc
     8221 tcacataaca tcgtgctgct gcaaaccatg cgcggtttct tcgatgtcct gcaatcctca
     8281 gtgaagcata gccgtcagcg gatgtatctg gtgccaccgg ttttttcaca actgaccgaa
     8341 caacatcagg ctgtcattga cgccattttt gctggtgatg ccgacggggc gcgtaaagca
     8401 atgatggcgc accttagttt tgttcacacc accatgaaac gattcgatga agatcaggct
     8461 cgccacgcac ggattacccg cctgcccggt gagcataatg agcattcgag ggagaaaaac
     8521 gcatgattat ttccgcagcc agcgattatc gcgccgcagc gcaacgcatt ctgccgccgt
     8581 tcctgttcca ctatatggat ggtggtgcat attctgaata cacgctgcgc cgcaacgtgg
     8641 aagatttgtc agaagtggca ctgcgccagc gtattctgaa aaacatgtcc gacttaagcc
     8701 tggaaacgac gctgtttaat gagaaattgt cgatgccggt agcactggca ccggtgggtt
     8761 tgtgtggcat gtatgcgcgt cgtggcgaag ttcaggcagc caaagcggcg gacgcgcatg
     8821 gcattccgtt tactctctcg acggtttccg tttgcccgat tgaagaagtc gcgccagcca
     8881 tcaagcgccc aatgtggttc cagctttatg tactgcgcga tcgcggcttt atgcgtaacg
     8941 cgctggagcg agcaaaagca gcgggttgtt cgacgctggt tttcaccgtg gatatgccga
     9001 caccgggcgc acgctaccgt gatgcgcatt caggtatgag cggcccaaat gcggcaatgc
     9061 gccgctactt gcaggcggtg acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc
     9121 gtccacatga tttaggtaat atctcagctt atctcggcaa accgaccgga ctggaagatt
     9181 acatcggctg gctggggaat aacttcgatc cgtccatctc atggaaagac cttgagtgga
     9241 tccgcgattt ctgggatggc ccgatggtga tcaaagggat cctcgatccg gaagatgcgc
     9301 gcgatgcagt acgttttggt gctgatggaa ttgtggtttc taaccacggt ggccgccagc
     9361 tggacggtgt actctcttcc gctcgtgcgt tgcccgctat tgcagatgcg gtgaaaggtg
     9421 atatcgccat tctggcggat agcggaattc gtaacgggct tgatgtcgtg cgtatgattg
     9481 cgctcggtgc cgacaccgta ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg
     9541 gccaggcggg tgtagctaac ctgctaaatc tgatcgaaaa agagatgaaa gtggcgatga
     9601 cgctgactgg cgcgaaatcg atcagtgaaa ttacgcaaga ttcgctggtg caggggctgg
     9661 gtaaagagtt gcctacggca ctggctccga tggcgaaagg gaatgcggca tagtcgtttg
     9721 cgccccctca ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9781 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9841 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
     9901 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
     9961 gcgctaatac cggctttcgt ctgcatatca tcgaaccgat gggatttgcc tgggacgata
    10021 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10081 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10141 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10201 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10261 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10321 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10381 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10441 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10501 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10561 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10621 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10681 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10741 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10801 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10861 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    10921 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    10981 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11041 ttccaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11101 catgtagctc agtgcactac caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11161 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11221 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11281 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtgg ctgctgcgct
    11341 cgtccttgcg tgcacgacct aataacgtca atgctgcctc gcgcgcgttt tttccgcaat
    11401 ataatacttg ataaatttcc tcggttattg gcatttctac gccgaagcga tgcgccagtt
    11461 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11521 tttgtacatc catgccctga ccgagcatca tgccaaaacg gcggttacgc gactggttgt
    11581 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11641 cacccagcgc cgcgccaaga cgcgacattt cagccagccc acgggtgatc agtgccgtac
    11701 gcgcattcgc accaaaaccg ataccgtcgg acatccccgc gccaatggca atgacgtttt
    11761 tcaccgcacc gccaagctgc acaccgatga aatccgggtt gctgtaaacg cggaaacttt
    11821 tgccacagtg cagcaattgt tggagatcat cggcaaaggt ctggtcggta gaggccaacg
    11881 aaatagccgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    11941 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12001 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12061 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacggctgg
    12121 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12181 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12241 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12301 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12361 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaat
    12421 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc acgctggtga tgcactcacg
    12481 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12541 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12601 ttcgcccagg gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12661 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12721 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12781 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12841 atactgcgca atgtaatgcc aacgttcaca cgttatttca gcaggggatc cagtccacca
    12901 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    12961 tggggaaccg tggtgcgacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13021 tcgatcggca actcctgaaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13081 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13141 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13201 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13261 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13321 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13381 tgcatgccag aaccgtctac cacgataacc ggtttgtctt tgtgcttctc aagctcacca
    13441 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13501 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13561 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13621 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13681 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13741 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13801 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13861 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    13921 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    13981 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14041 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14101 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14161 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14221 tttgctaatc cagtgctgac tggcgcggta gataaagcga aaaacgcagg taaagcagta
    14281 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14341 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14401 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14461 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14521 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14581 tttcaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14641 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14701 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14761 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14821 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    14881 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    14941 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15001 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15061 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15121 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15181 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15241 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15301 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15361 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15421 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15481 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15541 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15601 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15661 gccacaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15721 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15781 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15841 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    15901 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    15961 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16021 caaacgcgtg aagaagtcgc catgcagcgt gctgaactgg aagagaaaca aagcgagcaa
    16081 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16141 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16201 gaactgcgtg ccaacgaatc ccgtctgcgt aacagtattg cccgtgcgga agccgcggcg
    16261 aaagcgcgtg ctgaacgaga agcgcgtgag gctcaggcag ttcgcgacag gcagaaagaa
    16321 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16381 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cagttcgtgg ccctacgctg
    16441 catcgctatg gcgagcagct acagggtgaa ttacgctgga aaggaatggt tatcggtgcc
    16501 tctgaaggta ctgaagttaa agcaattgcc gacggtcggg tgattctggc tgactggctg
    16561 caaggctacg gtctggtggt ggtggttgag cacggtaaag gcgatatgag tctttacggc
    16621 tataatcaaa gcgcgctggt gagcgttggt tcgcaggttc gtgcgggcca gccaattgca
    16681 ctggtgggca gcagtggcgg tcagggcaga ccttcactct atttcgaaat tcgccgccag
    16741 ggtcaggccg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16801 aacgttcttg catttgccgc tctgttggcg ctctcctccc ctgtaattgc tggcaaactt
    16861 gccatcgtca ttgatgattt tgggtatcgc ccgaacaacg aaaaccaggt gctggcgatg
    16921 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    16981 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17041 cagccgctgg agaaaaatac gctgcgcccg gagatgagca gcgacgaaat tgagcgcatt
    17101 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaataacca catgggcagc
    17161 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttataat
    17221 ctttacttcc tcgacagcgt aaccattggt aatacccagg cgatgcgcgc cgcgcaaggc
    17281 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcacagaa cgaagcggac
    17341 atccgtgtgc aattcaatcg cgcaattgac ctagcgcgtc gcaacggatc aaccattgcc
    17401 attgggcatc ctcacccttc aacggtacgc gtgttgcaac agatggttta taacctgccg
    17461 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacgtct
    17521 acaccgccga aaaacactgt gcccgacaca ccgcgtaatc cgttccgtgg cgtgaagctg
    17581 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17641 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17701 aaacagcctg aagcggcgaa gcttaacgct agcgtaaatt aaaggcgcta cgcgtaatgc
    17761 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17821 gataacccac ctttacgctg cgcacattgg taatcaggcg cttatacata
//
LOCUS       DAZOZY010000075        17447 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZOZY010000075 DAZOZY010000000
VERSION     DAZOZY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17447
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.075"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..501)
                     /locus_tag="ACHURQ_002182"
     CDS             complement(<1..501)
                     /locus_tag="ACHURQ_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0823948.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(687..771)
                     /locus_tag="ACHURQ_002183"
     tRNA            complement(687..771)
                     /locus_tag="ACHURQ_002183"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Leu,seq:caa)
     gene            967..1986
                     /gene="ahr"
                     /locus_tag="ACHURQ_002184"
     CDS             967..1986
                     /gene="ahr"
                     /locus_tag="ACHURQ_002184"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR0823949.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHURQ_002185"
     CDS             complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHURQ_002185"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0823950.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2770..3801
                     /gene="idnD"
                     /locus_tag="ACHURQ_002186"
     CDS             2770..3801
                     /gene="idnD"
                     /locus_tag="ACHURQ_002186"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR0823951.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3825..4589
                     /gene="idnO"
                     /locus_tag="ACHURQ_002187"
     CDS             3825..4589
                     /gene="idnO"
                     /locus_tag="ACHURQ_002187"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR0823952.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4654..5973
                     /gene="idnT"
                     /locus_tag="ACHURQ_002188"
     CDS             4654..5973
                     /gene="idnT"
                     /locus_tag="ACHURQ_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR0823953.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6040..7038
                     /gene="idnR"
                     /locus_tag="ACHURQ_002189"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6040..7038
                     /gene="idnR"
                     /locus_tag="ACHURQ_002189"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR0823954.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7116..8618
                     /locus_tag="ACHURQ_002190"
     CDS             7116..8618
                     /locus_tag="ACHURQ_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR0823955.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHURQ_002191"
     CDS             complement(8737..9819)
                     /gene="lptG"
                     /locus_tag="ACHURQ_002191"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR0823956.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHURQ_002192"
     CDS             complement(9819..10919)
                     /gene="lptF"
                     /locus_tag="ACHURQ_002192"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR0823957.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHURQ_002193"
     CDS             complement(10982..11032)
                     /gene="ytgA"
                     /locus_tag="ACHURQ_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR0823958.1"
                     /translation="MSIIILATISTTQENK"
     gene            11186..12697
                     /gene="pepA"
                     /locus_tag="ACHURQ_002194"
     CDS             11186..12697
                     /gene="pepA"
                     /locus_tag="ACHURQ_002194"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR0823959.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12831..13274
                     /gene="holC"
                     /locus_tag="ACHURQ_002195"
     CDS             12831..13274
                     /gene="holC"
                     /locus_tag="ACHURQ_002195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR0823960.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13274..16129
                     /gene="valS"
                     /locus_tag="ACHURQ_002196"
     CDS             13274..16129
                     /gene="valS"
                     /locus_tag="ACHURQ_002196"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR0823961.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16185..17381)
                     /locus_tag="ACHURQ_002197"
     CDS             complement(16185..17381)
                     /locus_tag="ACHURQ_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR0823962.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4483 a         4336 c         4454 g         4174 t
ORIGIN      
        1 gcgctgttga agacgagcag ccacttcaag acgtccagac atttctacgg ccttaatagg
       61 tgctaataaa tctcgagttt ttaactcagc gatattacgt tcaccaagcg ctgcaaaaag
      121 attatcttcc agacttttta gcacacgatg ggcatgatct tcagaccact ttttattggt
      181 gccatgccac tcaatcgcga cttctttaaa ggttcgtgct ttactctgtt caaccttatc
      241 acttttcttt ttgtctcccg gatcgacgcc attcgcaagc agcttacgtg gctcgtcacg
      301 acgtattctg gcatccgcga gtgtgatttc aggataaacc ccaagtgcca gcatcttttg
      361 ctttccctca taacggtact gcaaacgcca gtacttagaa ccatttggat ggacaagcag
      421 atgcataccg ccaccatcag tcaacttata ttgcttatct gttggcttag ctgttctgac
      481 tttgatgtct gttaatgcca tgcgtattcc ctccctgttg gtacaagcat tatcgaaccg
      541 gaaataccat catctgtacc aacatatgtt ggtagaagta cgttgatatc ggttgacctt
      601 gagagagtta atatagcggg aaaggtagat aaatactgaa tttcaggcat aaaaaagacc
      661 tcagttgagg tctattgaca tacttttggt gccgaaggcc ggactcgaac cggcacgtat
      721 ttctacggtt gattttgaat caactgcgtc taccgatttc gccacttcgg cactgaagag
      781 gtatgcggaa aacgttgtgg attatacctg tcgcacgcca ccatgcaagc gccagcttgc
      841 tcgcacctcg ctaagtgctg aaattttcag cattgcatac agcgatgtgt aacctttgtc
      901 acactccagg caccccgccc tgccatgctc tacacttccc agacaacacc agagaaggac
      961 caaaaaatgt cgatgataaa aagctatgcc gcaaaagaag cgggcggcga actggaagtt
     1021 tatgagtacg agcccggtga attgaagcca caagatgttg aagtgcaggt ggattactgc
     1081 gggatctgcc attccgatct gtcgatgatc gacaacgaat ggggattttc acagtatccg
     1141 ctggttgccg ggcatgaggt gattgggcgc gtggtggcgc tcgggagcgc cgcgcaggat
     1201 aaaggtttgc aggtcggtca gcgtgtcggg attggctgga cggcgcgtag ctgcgggcat
     1261 tgcgacgcct gtattagcgg taatcagatc aactgcgagc aaggtgcggt gccaacaatt
     1321 atgaatcgcg gcggttttgc cgagaagttg cgtgcagact ggcaatgggt gatcccactg
     1381 ccggaaaata tcgatatcga gtccgccggg ccgctgctgt gtggtggtat cacggtcttt
     1441 aaaccactgt tgatgcacca tatcactgct accagccgcg ttggggtaat tggtattggc
     1501 gggctggggc atatcgctat aaaacttctg cacgcaatgg gatgcgaggt gacagccttt
     1561 agttcgaatc ctgcgaaaga gcaggaagta ctggcgatgg gtgccgataa agtggtgaat
     1621 agccgcgatc cgcaggcact gaaaacactt gcggggcagt tcgatctcat tatcaacacc
     1681 gtcaacgtca gcctcgactg gcagccttat tttgaggcgc tgacgtatgg cggtaatttc
     1741 cacaccgtcg gtgcggtcct cacgccgctg tctgttccgg cctttacgtt aattgcgggc
     1801 gaccgcagcg tctctggttc agcaaccggc acaccttatg agcttcgtaa gctgatgcgc
     1861 tttgcggccc gcagcaaggt tgcgccgacc accgaactgt tcccgatgtc gaaaataaac
     1921 gacgccatcc agcatgtgcg caacggtaag gcgcgttacc gcgtggttct gaaagccgat
     1981 ttttgaaaat cattcgcagc gctgatctga ggcgctaccc cctttcgcac atattctgtc
     2041 ttgtcgtatc gccagcacgg cctgccgaca ttgttcggtg acattggcaa tatcatggtt
     2101 gatatcaatg cgtacaatat cctgttcatc tgcttgtgga cgctccagcg cctcaaactg
     2161 actttttagt aacgctaccg gcataaaatg cccagcccgc cgctgcattc gcgcgagaat
     2221 agtttcatag tcgccatcta accagaggaa atgaacatgg gggctaccct tgcgtaaaat
     2281 atcacgatac tgttttttta atgatgaaca gacaataaat cctgtttcat tctttttata
     2341 aagactgtat gaagcatcat ttaagcgttc cagccaggga agtcgatctt catcagataa
     2401 tggaataccc tgcgacattt tatctatatt tttggctgga tgaagatcgt caccatcaat
     2461 aaatttagca gataataacg cggcaacctt gctaccaatt aatgttttac cactccctga
     2521 aacgcccatc aaaataaagc tttcacccgc catttaaaat ctcctgaatc atcttacctg
     2581 ctgcgatact acgcctggtg gcataataat ttcacgtttt tttatttctg ctgataagaa
     2641 ttacaaggca catcacgtta tgcgtaacat agtaatgtaa caattttctg acgtgatctt
     2701 catcacaaat aatgacaatt aaaaccgctt aaatgcttcc gaggtgtcta cctgaccagt
     2761 gaggaattta tgcaagtgaa aacacagtcc tgcgttgttg cggacaagaa aactgttgcc
     2821 gttaccgagc agacgataga ttggaataat aatggaacat tagtacaaat aacccgaggt
     2881 ggaatttgcg gttccgattt acattattat caggaaggaa aagtaggtaa tttcatgata
     2941 aaggcaccga tggtgttagg tcatgaagtt atcggtaaag ttattcatag cgactcatca
     3001 gaattacatg aagggcaaac ggtagccatt aatccgtcta aaccgtgcgg tcactgcaaa
     3061 tactgcattg aacataacga gaatcagtgt acagatatgc gtttttttgg cagtgccatg
     3121 tatttccctc atgttgatgg tggttttacc cgttataaaa tggtcgaaac gtcgcaatgt
     3181 gtcccttatc cggccaaagc tgacgaaaag gttatggctt ttgccgaacc tttagccgtc
     3241 gcgattcatg ccgcacatca ggccgacgag ttacagggca agcgagtatt tgtttccggt
     3301 gttgggccca ttggctgcct gattgtcagt gctgtgaaaa cactggggtc cgcggaaatt
     3361 gtctgtgccg atgtgagtcc ccgttccctt tcgctggcca aagagatggg tgcggatgtg
     3421 ctcgtaaacc cacaaaacga cgacatggat cactggaaag cggaaaaagg ccatttcgat
     3481 gtcagctttg aagtgtccgg tcatccttca tcagtgaata cctgtctgga ggtcactcgt
     3541 gcacgcggcg taatggtgca ggtagggatg ggaggcgcga tggcagaatt cccaatgatg
     3601 acgttgattg gtaaggagat ttcactcaaa ggctctttcc gttttaccag cgaatttaat
     3661 accgcagtgt catggctggc gaatggcgtt atcaatccac tgcctttact gagtgctgaa
     3721 tatctcttca ctgacctgga agaggcgcta cgtttcgccg gtgataaaac ccaggcagca
     3781 aaagtccagc ttgttttcta agagataaat aaaggaataa tacaatgaac gatctatttt
     3841 cactggcagg aaaaaatatc ttgattaccg gttcagcaca gggcattggc tatttactgg
     3901 caaccggcct gggtaaatat ggcgcacaaa taattattaa tgatattact gccgaacgcg
     3961 cagaacttgc tgtagaaaaa ctccaccagg agggtattca ggccgttgcc gcacctttta
     4021 atgttactca taaacatgaa attgatgccg ccgttgaaca tatcgaaaag gacatcggcc
     4081 ccattgatgt gctggtgaat aacgccggta tccagcgccg tcatcctttt actgagttcc
     4141 ctgaacaaga gtggaatgat gtgatcgcag taaaccagac cgccgtgttc ctggtatcgc
     4201 aagcggtaac tcgtcacatg gttgaacgca aggcaggtaa agttattaat atttgctcga
     4261 tgcaaagcga actgggacgt gacaccatca ccccttatgc cgcatcgaaa ggggcggtaa
     4321 aaatgctcac ccgcggcatg tgcgtcgagc tggcgcgcca caatattcag gtcaacggta
     4381 ttgcgccggg ctatttcaaa acagaaatga ctaaagcact ggttgaggac gaagccttca
     4441 ccgcctggtt gtgcaaacgg acccccgccg cacgctgggg agatccgcag gaactgattg
     4501 gtgctgcggt gttcctttct tcaaaagcct ctgatttcgt aaacggccac ctgttgtttg
     4561 ttgatggcgg catgttagtg gctgtttaac agccaccacc ctacgtgcag cgcgaaagcg
     4621 ctgcttttct ggcactaata agagataaag actatgccat taatcattat tgcggcaggc
     4681 gtcgcgctgc ttcttatcct gatgatcggc tttaaagtta acggctttat tgccctcgtt
     4741 ctggtagctg ccgtcgtcgg atttgccgaa gggatggatg cacaggccgt cctgcactct
     4801 atacaaaatg gtatcggcag cacgctcggc gggctggcaa tgatcctcgg tttcggggcc
     4861 atgttaggca agctgatttc tgatacgggc gcggcacaac gtatcgccac tacgctgatt
     4921 gctacttttg gtaaaaaacg cgtgcaatgg gcgctagtga tcaccggtct ggttgtgggc
     4981 ctcgccatgt tttttgaagt gggttttgtc ctgctgttgc cgttggtatt taccatcgtg
     5041 gcatcatctg gattacccct gttgtatgtt ggcgtaccga tggtagcagc gctctctgta
     5101 acccactgtt ttctgccgcc acatccaggg cctactgcca tcgcgactat ctttgaggct
     5161 aatctcggaa cgactttact gtatggattt atcattacca ttccgacagt tattgtcgca
     5221 ggaccgctgt tttctaaact gctaactcgc tttgagaaag caccaccgga aggcttattt
     5281 aatcctcatc tgtttagcga agaggagatg ccctccttct ggaacagtat ttttgctgca
     5341 gtgatcccgg tcatcctgat ggctatcgcc gccgtttgtg aaattacgtt accgaaaact
     5401 aacaccgtgc gcctcttctt tgaatttgtc ggtaaccctg ccgttgcgct gtttattgcc
     5461 attgttattg cgattttcac actgggccga cgtaatggac gcaccatcga gcaaatcatg
     5521 gatatcattg gggattctat aggcgctatc gcgatgattg tgtttattat cgctggcggc
     5581 ggcgcgttta agcaggtatt agtagatagt ggtgtcgggc agtatatttc acacttaatg
     5641 accggaacta cgctttcgcc gttattgatg tgctggactg tcgcggcgct gttgcgtatc
     5701 gctctgggct ctgccaccgt cgcggccatt accaccgcgg gtgtggtgtt gccgattatc
     5761 aacgttaccc atgccgatcc cgctttaatg gtactggcaa ctggtgcggg cagcgtgatc
     5821 gcgtcacacg taaacgaccc tggcttctgg ctatttaaag ggtattttaa tctgacggtt
     5881 ggtgaaacgt tgcgtacctg gacagtgatg gaaaccctta tttctattat gggtttgctg
     5941 ggcgtgttag ccattaacgc cgtattgcac tgaaagaata aaatattgtg atgtagtttc
     6001 gggggaagat tgaagattcc cccgacgttg ggagaagtta tgcgcaatca cagaatttct
     6061 ttacaggata tcgctacgct ggctggcgta acaaaaatga ccgtgagtcg ttatatccgc
     6121 tcgccgaaaa aggtggcaaa ggaaacaggc gagcgcatcg ccaaaattat ggaagagatc
     6181 aattatatcc ctaatcgcgc tcccggcatg ttgttgaatg cacaaagtta taccctcggt
     6241 atcttgattc cgtcatttca aaatcaactc ttcgctgaca ttctcgcggg aatagaatcc
     6301 gtcacctctg tgcataatta tcagaccctt attgctaatt acaattacga tcgcgattcc
     6361 gaagaagagt cggtgattaa cctgctctct tataacattg acgggattat tctttcggaa
     6421 aaatatcaca ccatcaggac ggtgaaattt ctgcgttcag ccaccattcc ggtcgtggaa
     6481 ttgatggatg tacagggaga acggctggat atggaggtcg gttttgataa tcggcaggct
     6541 gcttttgaca tggtgtgtac catgctggat aagcgagtca ggcgcaaaat tctgtatctc
     6601 ggttccaaag atgacacccg cgatgaacag cgttatcagg ggtattgcga tgcgatgatg
     6661 ctgcataatc tttccccatt acgcatgaat ccacgcgcca tctcatccat tcatttagga
     6721 atgcagttaa tgcgtgatgc ccttagcgca aacccggatt tagacggtgt attttgcacc
     6781 aatgacgata ttgcgatggg tgcgcttctg ctgtgccgcg agcgaaacct cgccgtgcca
     6841 gaacaaattt ccattgcggg ttttcatggg ctggagattg gcaggcaaat gatcccaagc
     6901 ctcgccagcg taattactcc ccgttttgat attggtcgga tggccgccca aatgttgctg
     6961 agcaagatta agaataacga tcacaaccac aacactgttg acctcgggta tcagatttat
     7021 cacggtaaca cgctttaggt tattttctct tcattacaca tattctcttt gccggacgtt
     7081 gcctatactc caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc
     7141 gcacgccgga cacagaactg tttttactgc cgggaatggc taaccgtcac gggctgatta
     7201 ctggcgcaac ggggacgggt aaaactgtca cgttgcaaaa actggcagag tcattgtcgg
     7261 aaatcggtgt tccggtgttt atggccgatg tgaaaggcga tctgaccggc atcgcgcagg
     7321 caggaacggc gtcggaaaaa ctgctcgcaa gactgaaaaa tatcggcgtc aacgactggc
     7381 aaccgcatgc caatccggtg gtggtgtggg atatctttgg cgagaaaggc catccggtgc
     7441 gggcgacggt ttcggatctg gggccgctgt tgctggcacg actgttgaat ctcaacgatg
     7501 tgcaatctgg cgtgctgaat attatcttcc gcattgcgga cgatcaggga ttgttgctgc
     7561 tcgactttaa agatttgcgg gcgattaccc agtacatcgg cgataacgcc aaatccttcc
     7621 agaatcagta cggaaatatc agtagcgcat cggttggtgc catccagcgc ggattactgt
     7681 cgctggaaca gcaaggcgca gaacacttct ttggtgagcc gatgctggat atcaaagact
     7741 ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaaacttt
     7801 atcagatgcc gaaactgtac gccgccagcc tgctgtggat gctttcagag ttatatgaac
     7861 aattgcctga agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaagcac
     7921 atctgctgtt taacgatgcg ccacaggtac tgctggataa gattgagcag gtgataaggc
     7981 ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtca gatatcccgg
     8041 ataacgtgct agggcagctc ggtaatcgcg ttcaacacgc tttgcgtgct tttacgccta
     8101 aagatcagaa agcagtgaag gcagcggcgc aaaccatgcg ggccaatccg gcgtttgata
     8161 ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt ctcgatgcga
     8221 aaggaagtcc ttcagtggtg gaacgggcga tggtgatcgc accttgttcg cgaatggggc
     8281 cggtgacgga agatgagcgt aatggcctga ttaatcactc cccggtgtat ggcaaatatg
     8341 aagatgaggt ggaccgcgag tccgcctatg agatgctgca aaaaggcttt caggccagta
     8401 ccgagcagca aaataatccc cccgtgaaag ggaaagaggt ggcggtggat gacggtattc
     8461 ttggtggatt gaaggatatt ttgtttggca ctaccggacc acgcggcggg aagaaagatg
     8521 gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt gacgaatcag attgtacgcg
     8581 ggatgttggg gagtttgctg ggggggagaa aaaggtaagt cagacgtgat ctgtgcccct
     8641 caccctaacc ctctccccaa aggggcgagg ggaccgttcg aactcgtatt tgactttgtc
     8701 aacaataata aagggagctt tcgctccctt gttagattac gattttctca ttaacagcca
     8761 caggctgatt aagaagaagc tggcgcttgg caacagtgcg ccgatgatcg gcgggatgcc
     8821 ataaaccaac gtcagcgggc cgaagatctg gtccagtacg tagaagacaa aaccgaagct
     8881 gataccggtg accacacgca cgcccatcgg tacgctacgc agtgggccaa agatgaacga
     8941 cagcgccatc aacatcatca ccgccacgga tagcggctgg aagattttgc tccacatgtt
     9001 gagctgataa cgtccggcat cctgaccgct cgacttcaga tacttcacat agttgtgcaa
     9061 accactgatg gagagtgcat ccgggtccag cgccaccacg cccagtttgt ctggcgtgag
     9121 gttggttttc caggtgccgc tcaccgtctg cgaaccggtg atctgtttcg gattggtcag
     9181 atcagattca tcaacctgcg acagacgcca gactttatgt tccgggtcaa acttcgcggt
     9241 agcggcataa cgtacggatt gcagacgacg attctcgtta aaggcataaa tactgatgcc
     9301 acctaactct tcgtcacctt taacccgctc aatgtagacg aagttgttgc catctttcgc
     9361 ccataagcct tgctgagtgg agagcaacga gccgccgtac atcgcctgcg cacggtagtt
     9421 acgcgccatc tgctcgccct gcgacgcgac ccattcacca atcgccatcg tcagcaagac
     9481 cagcggaatg gcggttttca tcaccgacag cgccacctgc atacgggtaa aaccagaagc
     9541 ctgcatcacc accagttcgc tgcgctgcgc cagcatccca agaccaagca acgccccaag
     9601 cagagccgcc atcgggaaga agatttgcac atctttcggc acgctcagca aggtatacat
     9661 tcctgcgcct aacgcgtcgt aactcccctg cccggctttt ttcagctgat cgacaaactt
     9721 gataatgccc gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg tgaaaatagt
     9781 tttaccgata tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc tttacgcgaa
     9841 aaactggcgc gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac aatcgccaaa
     9901 gccagataaa tcaggttaac ggtccacatc cacagcgtcg ggtccagctt acctttaccg
     9961 ccgttcgatt tcagggaggt ctggatcagg aagaaaagta gatacagcag catggctggc
    10021 agcatcgaca gtacgcgtcc ctgacgcggg ttaaccacgc tcaacggtac aaccataagt
    10081 gccatcataa acacggtgaa taccaacgtg atacgccagt tcagttctgc gcgggcacga
    10141 tcggtgtcag tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt cgggtcgagc
    10201 accaccgcct ggtgaccaat gatcgcctga taatcctgaa agtccgtaat gcggaaatcg
    10261 cgtaacagtg cagtgccttc gaagcgcgtt ccctggttga gagtgacgac ctgggagccg
    10321 tcgcgcagct gggttaaatg tccggaatcg gccaccacca cagaaggacg tgcattacct
    10381 tttggtcgaa tttgtgcgag gaacacatct ttgaaatcgc tgccgtcaac gctttcgatg
    10441 aacagcaccg agctgccatt agtcgcttgc tggaattgcc cttgcgccag cgccgccatg
    10501 ccagggttcg ctttcgcttc tgctaacact tcatcctgat gacgcgatga ccatggtccc
    10561 gcccacatca cgttaaccgc cgcgacgatt gccgtgaaca ccgcaaggat cattgccgct
    10621 ttcaccagca ccgctttgct cagaccgcag gcatgcatta ccgtaatttc actttcggta
    10681 tacagtttgc ccagcgtcat cagcagcccg aggaacaggc ttaatggcag gataagctgc
    10741 gccatttccg gcacgcccaa cccgagaagg gagagcacca gattcgccgg aatatcgccg
    10801 tcaaccgctg cgccgaggat cctcactaac ttttgacaga agaagatcaa aagcaagatg
    10861 aagagtatcg ccagctggct tttgagcgtc tcccgcacca gatatcttat gattatcact
    10921 ttaaatacgc ccgtaaaaac tcgtcttttg caggatttta gcttgtttca tggcttaaac
    10981 gtcatttatt ctcttgagtc gtcgaaatcg tcgctaagat aattatactc aacggattca
    11041 cctctcagat tttgttctga cgtgccaatg ccgtaataac gttaagatta acacgaagtc
    11101 atcgcaacag cggacatgag ttacgaaagc ttgcaattct atctgtagcc accgccgttg
    11161 tctttaagat tcaggagcgt agtgcatgga gtttagtgta aaaagcggta gcccggagaa
    11221 acagcggagt gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt ctccgattgc
    11281 agaacagctc gataaaatca gcgatgggta catcagcgcc ctgctacgtc ggggcgaact
    11341 ggaaggaaaa ccggggcaga cactgttgct gcaccatgtt ccgaatgtac tttccgagcg
    11401 aattctcctt attggttgcg gcaaagaacg tgagctggat gaacgtcagt acaagcaggt
    11461 tattcagaaa accattaata cgctgaatga tactggctca atggaagcgg tctgctttct
    11521 gactgaactg cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg ctgtcgagac
    11581 tgcaaaagag acgctctaca gtttcgatca gctgaaaacg aacaagagcg aaccgcgtcg
    11641 tccgctgcgt aaaatggtgt tcaacgtgcc gacccgccgt gaactgacca gcggtgagcg
    11701 cgcgatccag cacggtctgg cgattgccgc cgggattaaa gcagcaaaag atctcggcaa
    11761 tatgccgccg aatatctgta acgccgctta cctcgcttca caagcgcgcc agctggctga
    11821 cagctacagc aagaatgtta tcacccgcgt tatcggcgaa cagcagatga aagagctggg
    11881 gatgcactct tatctggcgg tcggtcaggg ttcgcaaaac gaatcgctga tgtcggtgat
    11941 tgagtacaaa ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg gtaaaggttt
    12001 aaccttcgac tccggcggta tctccatcaa gccttcagaa ggcatggatg agatgaagta
    12061 cgatatgtgc ggtgcggcag cggtttacgg cgtgatgcgg atggtcgcag agctacaact
    12121 gcctattaac gttattggcg tgctggcagg ctgtgaaaac atgcctggcg ggcgtgccta
    12181 tcgtccgggc gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc tgaacaccga
    12241 tgccgaaggc cgcctggtac tgtgcgacgt gttaacttac gttgaacgtt ttgagccgga
    12301 agcggtgatt gatgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg gtcatcatat
    12361 tactggtctg atggcgaacc ataatccgct ggcccatgaa ctgattgccg cgtctgaaca
    12421 atccggtgac cgcgcatggc gcttaccgct gggtgacgag tatcaggaac aactggagtc
    12481 caattttgcc gatatggcga acattggcgg tcgtcctggt ggggcgatta ccgcaggttg
    12541 cttcctgtca cgctttaccc gtaagtacaa ctgggcgcac ctggatattg caggaaccgc
    12601 ctggcgttct ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc tggcacagtt
    12661 cctgttaaac cgcgctgggt ttaacggcga agagtaagtt tgcgttttac cctcacccta
    12721 accctctccc taagggagag ggaaccgatc gagcgcaata gaaattcatt gctcatcttt
    12781 cagggtgagg aatgcagcaa tatttaaatc caccacaaga agccccatat atgaaaaacg
    12841 caacgttcta ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac
    12901 tggtgtgtga aattgccgca gaacgttggc gcagcggtaa gcgcgtgctc atcgcctgtg
    12961 aagatgaaaa gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct
    13021 ttgtcccgca taatttagcg ggagaaggac cgcgcggcgg tgcgccggtg gagatcgcct
    13081 ggccgcaaaa acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg
    13141 cagattttgc cactgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga
    13201 aacaactggc gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg
    13261 caacctggaa ataatggaaa agacatataa cccacaagat atcgaacagc cgctttacga
    13321 gcactgggaa aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg
    13381 catcatgatc ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca
    13441 aaccatcatg gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctatggca
    13501 ggtcggtact gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc
    13561 agaagaaggt aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga
    13621 atggaaagcg gagtccggcg gcaccattac ccgtcagatg cgccgtctcg gcaactctgt
    13681 cgactgggag cgtgaacgct tcacgatgga cgaaggcctg tccaatgcgg tgaaagaagt
    13741 tttcgttcgt ctgtataaag aagacctgat ttaccgtggc aaacgcctgg taaactggga
    13801 tccgaaactg cgcaccgcta tctctgacct ggaagtggaa aaccgcgaat cgaaaggttc
    13861 gatgtggcac atccgctatc cgctggctga cggcgcgaaa accgcagacg gtaaagatta
    13921 tctggtggtt gcgactaccc gtccggaaac cctgttgggc gataccggcg tggccgttaa
    13981 cccggaagat ccgcgttaca aagacctgat tggcaaatat gtcattctac cgctggttaa
    14041 ccgtcgtatt ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt
    14101 gaaaatcact ccggcgcacg actttaacga ctatgaagtg ggtaaacgtc acgcgctgcc
    14161 gatgatcaac atcctgacct ttgacggcga tatccgtgaa agcgcccagg tgttcgatac
    14221 caaaggtaac gaatctgacg tttattccag cgaaatcccg gcagagttcc agaaactcga
    14281 gcgttttgct gcacgtaaag cagtcgttgc tgcggttgac gcactcggcc tgctggaaga
    14341 aattaaaccg cacgacctga ccgttcctta cggcgaccgt ggcggcgtag ttatcgaacc
    14401 aatgctgacc gaccagtggt acgtgcgtgc cgatgtactg gcgaaaccgg cggttgaagc
    14461 ggttgagaac ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg
    14521 gatgcgcgat attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc
    14581 tgcatggtac gacgaagcag gtaacgttta tgttggccgc aacgaagaag aagtgcgcaa
    14641 ggaaaataac ctcggtgccg acgttgccct gcgtcaggac gaagacgttc tcgacacctg
    14701 gttctcttct gcgctgtgga ccttctctac cctaggctgg ccggaaaaca ctgacgcact
    14761 gcgccagttc cacccaacca gcgtgatggt atccggcttc gacatcattt tcttctggat
    14821 tgcccgcatg atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt
    14881 gccgttccac accgtttaca tgaccggcct gattcgtgac gacgaaggcc agaagatgtc
    14941 caaatccaag ggtaacgtta ttgacccgct ggatatggtt gacggtattt cgctgccgga
    15001 actgctggag aaacgtaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa
    15061 gcgcaccgag aagcagttcc cgaacggtat tgagccgcac ggcaccgatg ccctgcgctt
    15121 caccctggcg gcgctggcgt ctaccggtcg tgacatcaac tgggatatga agcgcctgga
    15181 aggttaccgt aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac
    15241 ggaaggtcag gattgcggct tcaacggtgg tgaaatgacg ctgtcgctgg cggaccgctg
    15301 gattctggca gagttcaacc agaccatcaa agcgtaccgc gaagcgctgg acagcttccg
    15361 cttcgatatc gctgcaggca ttctgtatga gttcacctgg aaccagttct gtgactggta
    15421 tctggagctg actaagccgg taatgaacgg tggcaccgaa gccgaactgc gcggtactcg
    15481 ccatacgctg gtgaccgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt
    15541 catcaccgaa accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat
    15601 catgctgcag ccgttcccgc agtacgatgc atctcaggtt gatgaagccg cactggccga
    15661 caccgaatgg ctgaaacagg cgatcgttgc ggtacgtaac atccgtgcag aaatgaatat
    15721 cgcgccgggc aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg
    15781 cgtaaatgaa aaccgtggct tcctgcaaac cctggcgcgt ctggaaagca tcaccgtgct
    15841 gcctgccgat gacaaaggtc cggtttccgt tacgaagatc atcgacggcg cagagctgct
    15901 gatcccgatg gctggcctca tcaacaaaga agatgagctg gcgcgtctgg cgaaagaagt
    15961 ggcgaagatc gaaggcgaaa tcagccgtat cgagaacaag ctggcgaatg aaggctttgt
    16021 cgcccgcgca ccggaagcgg tcatcgcgaa agagcgtgag aagctggaag gctatgcgga
    16081 agcgaaagcg aaactgattg aacagcaggc tgttatcgcc gcgctgtaat caaaacacag
    16141 tgatgaaaac gaaggccgga gcatgctccg gcctttttta tctcttacag aaatggtaaa
    16201 gaaggcataa ttccccgtga aatactggct aagaatcctt tatctggttg ttcttcttta
    16261 ttgattaaag gaaggtcatc caaatcgccc agcaaatacg tattttttgc ctgccagtca
    16321 atcatccata ttttcagcag tggataagcc agaccgcccg taatcccgga tatcaccacc
    16381 aacgcacaca tgcgataaag cataccgtgg tatgttaaag ttgagcgaaa acgaatacgc
    16441 ccatcattca gtgacaggtt gctcataaaa tgatttcgca acgacaccgt taaataactg
    16501 gtgctaacag caatcccaaa ataatagatt aactgcgcga ttatcatttt acgttgcatt
    16561 tccataaatt gctgtaaatt ctcaatatca tcatttgcat acccagaact atcatactca
    16621 tttaatattt gatcgaagat aatataacca gcaacagcga gaaaaggtaa taaagccaaa
    16681 aatgcgagaa tggcatattt tatacaatat gtagtatcta atttcacctt gaaacgatgt
    16741 ataccgaaac tggtattact ccagagaaaa ctcattacca gactatataa agtgccatta
    16801 aaaataccaa tgaaaacaat acaaactatt gtcatcagaa ccacagatat aataacgcta
    16861 cttgaactat tggcatgtag catctttgtc gagataaaga aaacagtccc catcccaagg
    16921 gccattaaaa tcggcaagaa aaaggtcacc caccagaacc ctttcataga gcaattaaaa
    16981 ctaaatctta cgccgttgag actggtcatc aaggcctgat aacgcagtcc ttttgctgcc
    17041 ataaatataa gcaaaaccaa cagtgacaaa gtcaaaacac agccaataag cggcatatct
    17101 gctgacactg tcattaagat tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa
    17161 acattcccac cggtaatccc ataagaaaat cgttgtccgt taacttccat attagcatag
    17221 agataacgct tacatttcat taatgcccat ggtaaataga tacctagcgt tataatcgtt
    17281 aacaacacat tcaccacaca aataagaaaa tatctctcac ctgtaccatg aaaaacaaac
    17341 gaatgggacg gaacatccat atcattaata acttgagcca taaaagttcc ctgaattaaa
    17401 aaatccattg atataattta tatatacaga cagtaaaatg ttctgcc
//
LOCUS       DAZOZY010000076        17288 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZOZY010000076 DAZOZY010000000
VERSION     DAZOZY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.076"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..921
                     /gene="ybeQ"
                     /locus_tag="ACHURQ_002198"
     CDS             <1..921
                     /gene="ybeQ"
                     /locus_tag="ACHURQ_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR0823963.1"
                     /translation="AEKGDCEAQYIVGFYYNRDSAIDSPDDEKAFYWLKLAAEQGHCE
                     AQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASNALGWTLDRGEAPNYKEAVV
                     WYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYKQAALQGHSDAQNNLADLYE
                     DGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGEGVDQDYKQAMYWYLKAAAQ
                     ESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDATAWYNLAIMYHYGEGRPVD
                     LRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(991..1473)
                     /locus_tag="ACHURQ_002199"
     CDS             complement(991..1473)
                     /locus_tag="ACHURQ_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR0823964.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1708..4290
                     /gene="leuS"
                     /locus_tag="ACHURQ_002200"
     CDS             1708..4290
                     /gene="leuS"
                     /locus_tag="ACHURQ_002200"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR0823965.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4305..4886
                     /gene="lptE"
                     /locus_tag="ACHURQ_002201"
     CDS             4305..4886
                     /gene="lptE"
                     /locus_tag="ACHURQ_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR0823966.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            4886..5917
                     /gene="holA"
                     /locus_tag="ACHURQ_002202"
     CDS             4886..5917
                     /gene="holA"
                     /locus_tag="ACHURQ_002202"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR0823967.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            5919..6560
                     /gene="nadD"
                     /locus_tag="ACHURQ_002203"
     CDS             5919..6560
                     /gene="nadD"
                     /locus_tag="ACHURQ_002203"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR0823968.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6584..7195
                     /locus_tag="ACHURQ_002204"
                     /pseudo
     CDS             6584..7195
                     /locus_tag="ACHURQ_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            7455..7772
                     /gene="rsfS"
                     /locus_tag="ACHURQ_002205"
     CDS             7455..7772
                     /gene="rsfS"
                     /locus_tag="ACHURQ_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR0823969.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7776..8243
                     /gene="rlmH"
                     /locus_tag="ACHURQ_002206"
     CDS             7776..8243
                     /gene="rlmH"
                     /locus_tag="ACHURQ_002206"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR0823970.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8274..10175
                     /gene="mrdA"
                     /locus_tag="ACHURQ_002207"
     CDS             8274..10175
                     /gene="mrdA"
                     /locus_tag="ACHURQ_002207"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR0823971.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10178..11290
                     /gene="mrdB"
                     /locus_tag="ACHURQ_002208"
                     /gene_synonym="rodA"
     CDS             10178..11290
                     /gene="mrdB"
                     /locus_tag="ACHURQ_002208"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR0823972.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11301..12389
                     /gene="rlpA"
                     /locus_tag="ACHURQ_002209"
     CDS             11301..12389
                     /gene="rlpA"
                     /locus_tag="ACHURQ_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR0823973.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12528..13739
                     /gene="dacA"
                     /locus_tag="ACHURQ_002210"
     CDS             12528..13739
                     /gene="dacA"
                     /locus_tag="ACHURQ_002210"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR0823974.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13849..14112
                     /gene="ybeD"
                     /locus_tag="ACHURQ_002211"
     CDS             13849..14112
                     /gene="ybeD"
                     /locus_tag="ACHURQ_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR0823975.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14213..14854
                     /gene="lipB"
                     /locus_tag="ACHURQ_002212"
     CDS             14213..14854
                     /gene="lipB"
                     /locus_tag="ACHURQ_002212"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR0823976.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15114..16067
                     /locus_tag="ACHURQ_002213"
     CDS             15114..16067
                     /locus_tag="ACHURQ_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR0823977.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16276..17241
                     /gene="lipA"
                     /locus_tag="ACHURQ_002214"
     CDS             16276..17241
                     /gene="lipA"
                     /locus_tag="ACHURQ_002214"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR0823978.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         4305 a         4379 c         4444 g         4160 t
ORIGIN      
        1 gctgaaaaag gcgattgcga ggctcaatat attgttgggt tttattataa tcgcgatagc
       61 gcaattgact ctccggacga cgaaaaggcc ttttactggc tgaagctggc cgctgagcaa
      121 ggtcattgtg aagcacagta ttccttaggg caaaagtata ccgaggataa aagccgtcat
      181 aaagataatg agcaagccat cttctggctg aaaaaagctg ccctacaagg ccatactttc
      241 gcttccaacg cccttggctg gacactggat cgtggagaag cccccaatta taaagaagcg
      301 gttgtctggt atcagatagc cgcggagagc ggaatgtctt atgcgcaaaa taatcttggg
      361 tggatgtaca gaaatggcaa cggagtcgca aaagactatg cgctggcatt tttctggtac
      421 aaacaagctg cattacaagg ccatagtgac gcgcaaaaca atctggccga tctttatgaa
      481 gacggaaaag gcgttgctca aaacaagaca ctcgccgcat tctggtattt gaaaagcgca
      541 cagcagggta atcggcacgc ccagtttcaa attgcatggg attataacgc tggcgaaggg
      601 gtggaccagg actataagca agcgatgtac tggtatctga aggctgccgc tcaggaaagc
      661 gtcggcgctt acgtcaacat cggttatatg tataaacacg gacaaggcgt tgagaaagat
      721 tatcaggctg cctttgaatg gtttacgaaa gccgctgaat gcaatgacgc cactgcctgg
      781 tataacctgg ccattatgta tcactacgga gaaggaagac ctgtcgatct ccgacaggct
      841 ctcgacctgt atcgtaaagt tcagtcatcc ggaaccagag atgtcagtca ggaaattcgt
      901 gagactgaag atttactgta gagataaaaa caagggccgg ggaatttcat tcggcccata
      961 ttctctttat tgctgtgcga aatcagacta ttacggctca aacgggcgtc tctggaactg
     1021 gtcatgacca catttcgggc atagcgtcag cacttccggt gtgtagatcg ggagatggaa
     1081 gtgacatttc tcgcagacca gatttcccag cccgaccact tctccgctgt gataaacccc
     1141 atgatgattg aggtcctgga aaacttcgcg ccattcaagc tgcgttttat cggtgatatc
     1201 tgccagctcc tgccataagc tttctttaat cacccgcata aagacgctgt cagattcttc
     1261 tttcaggctc tcttcatagc tcatggcgaa ctcttccagg tcacgtctta cagctcgcgt
     1321 cagctcatcg acctcggttc gcgttaactc ccctgttttt attacgcgct cgcgcgcctg
     1381 ttccaccagt gcgtcgatat cacgttcgcc attgcgcagg cgttcgctca gtgacgcgac
     1441 cagttcacgg taatattgag caaccttgtt catcgtttca tctcccgggt cgttaactga
     1501 ctataataat agacgttctt tgttccttgc tttgctaata cgcccacaaa ctctgtaaat
     1561 taatgtatga ataatccggg catcatttca acgtcgagtg cgcgaaaggc tgttttgacg
     1621 cggacgtttg ggctatgcta tgcggatctg aaaaaccacc tcaacgctac atttgtagcc
     1681 gtattgaaaa caggaccact ggctgccatg caagagcaat accgcccgga agagatagaa
     1741 tccaaagtac agcttcattg ggatgagaag cgcacatttg aagtaaccga agacgagagc
     1801 aaagagaagt attactgcct gtctatgctt ccctatcctt ctggtcgact acacatgggc
     1861 cacgtacgta actacaccat cggtgacgtg atcgcccgct accagcgtat gctgggcaaa
     1921 aacgtcctgc agccgatcgg ctgggacgcg tttggtctgc ctgcggaagg cgcggcggtg
     1981 aaaaacaaca ccgctccggc accgtggacg tacgacaaca tcgcgtatat gaaaaaccag
     2041 ctcaaaatgc tgggctttgg ttatgactgg agccgcgagc tggcaacctg tacgccggaa
     2101 tactaccgtt gggaacagaa attcttcacc gagctgtata aaaaaggcct ggtatataag
     2161 aagacttctg cggtcaactg gtgcccgaac gaccagaccg tactggcgaa cgaacaagtt
     2221 atcgacggct gctgctggcg ctgcgatacc aaagttgaac gtaaagagat cccgcagtgg
     2281 tttatcaaaa tcactgctta cgctgacgag ctgctcaacg atctggataa actggatcac
     2341 tggccagaca ccgttaaaac catgcagcgt aactggatcg gtcgttccga aggcgtggag
     2401 atcaccttca acgttaacga ctatgacaac atgctgaccg tttacactac ccgcccggac
     2461 acctttatgg gttgtaccta cctggcggta gctgcgggtc atccgctggc gcagaaagcg
     2521 gcggaaaata atcctgaact ggcggccttt attgacgaat gccgtaacac caaagttgcc
     2581 gaagctgaaa tggcgacgat ggagaaaaaa ggcgtcgata ctggctttaa agctgttcac
     2641 ccattaacgg gcgaagaaat tcccgtttgg gcagcaaact tcgtattgat ggagtacggc
     2701 acgggcgcag ttatggcggt tccgggtcac gaccagcgcg actacgagtt tgcctctaaa
     2761 tacggcctga acatcaaacc agttatcctg gctgctgacg gctctgagcc agatctttct
     2821 cagcaagccc tgactgaaaa aggcgtgctg ttcaactctg gcgaattcaa cggtcttgac
     2881 catgaagcgg ccttcaacgc catcgccgat aaactgactg cgatgggcgt tggcgagcgt
     2941 aaagtgaact accgcctgcg cgactggggt gtttctcgtc agcgttactg gggcgcgccg
     3001 attccgatgg tgacgctgga agacggtacc gtaatgccga ccccggacga ccagctgccg
     3061 gtgatcctgc cggaagatgt ggtgatggac ggcattacca gcccgattaa agcagatccg
     3121 gagtgggcaa aaactaccgt taacggtatg ccagcgctgc gtgaaaccga cactttcgac
     3181 acctttatgg agtcctcctg gtactatgcg cgctacactt gcccagagta caaagaaggt
     3241 atgctggatt ccgaagcggc taactactgg ctgccggtgg atatctacat tggtggtatc
     3301 gaacacgcca ttatgcacct gctctacttc cgcttcttcc acaaactgat gcgtgatgca
     3361 ggcatggtga actctgacga accagcgaaa cagttgctgt gtcagggtat ggtgctggca
     3421 gatgccttct actatgttgg cgaaaacggc gaacgtaact gggtttcccc ggttgatgct
     3481 atcgttgaac gtgacgagaa aggccgtatc gtgaaagcga aagatgcggc aggccatgaa
     3541 ctggtttata ccggcatgag caaaatgtcc aagtcgaaga acaacggtat cgacccgcag
     3601 gtgatggttg aacgttacgg cgcggacacc gttcgtctgt ttatgatgtt tgcttctccg
     3661 gctgatatga ctctcgaatg gcaggaatcc ggtgtggaag gggctaaccg cttcctgaaa
     3721 cgtgtctgga aactggttta cgagcacaca gcaaaaggtg atgttgcggc actgaacgtt
     3781 gatgcgctga ctgaagatca gaaagcgctg cgtcgcgatg tgcataaaac gatcgctaaa
     3841 gtgaccgatg atatcggccg tcgtcagacc ttcaacaccg caattgcggc gattatggag
     3901 ctgatgaaca aactggcgaa agcaccaacc gatggcgagc aggatcgcgc tctgatgcag
     3961 gaagcgctgc tggccgttgt ccgtatgctt aacccgttca ccccgcacat ctgcttcacg
     4021 ctgtggcagg aactgaaagg cgaaggcgat atcgacaacg cgccgtggcc ggttgctgac
     4081 gaaaaagcga tggtggaaga ctccacgctg gtcgtggtgc aggttaacgg taaagtccgt
     4141 gccaaaatca ccgttctggt ggacgcaacg gaagaacagg ttcgcgaacg tgctggccag
     4201 gaacatctgg tagcaaaata tcttgatggc gttaccgtgc gtaaagtgat ttacgtacca
     4261 ggtaaactcc tcaatctggt cgttggctaa gcgcgggagg aagcgtgcga tatctggcaa
     4321 cattgttgtt atctctggcg gtgttaatca ccgccgggtg tggctggcat ctgcgtgata
     4381 ccacgcaggt tccttccact atgaaggtca tgatcctgga ctcaggcgat ccgaacgggc
     4441 cattaagccg tgcagtgcgt aaccagttac gtctgaatgg tgtcgagttg cttgataaag
     4501 aaaccacgcg taaggacgtt ccatccctgc gtttgggtaa agtgagcatc gcgaaagata
     4561 ccgcatcggt attccgtaac ggtcaaacag cagagtatca gatgatcatg acggtgaatg
     4621 cgaccgtgtt gatccccggc cgtgatatct acccgattag cgccaaagtc ttccgttcgt
     4681 tctttgataa cccgcaaatg gcgttagcga aagataacga acaagacatg atcgtaaaag
     4741 agatgtacga ccgtgctgcc gaacagctga ttcgtaagct gccaagcatc cgtgctgcgg
     4801 atattcgttc cgacgaagaa cagacgtcga ccacaacgga tactccggca acgcctgcac
     4861 gcgtctccac cacgctgggt aactgatgat tcggttgtac ccggaacaac tccgcgcgca
     4921 gctcaatgaa gggctgcgcg cggcgtatct tttacttggt aacgatcctc tgttattgca
     4981 ggaaagccag gacgctgttc gtcaggtagc tgcggcacaa ggattcgaag aacaccacac
     5041 tttttccatt gatcccaaca ctgactggaa tgcgatcttt tcgttatgcc aggctatgag
     5101 tctgtttgcc agtcgacaaa cgctattgct gttgttacca gaaaacggac cgaatgcggc
     5161 gatcaatgaa caacttctca cactcaccgg acttctgcat gacgacctgc tgttgatcgt
     5221 ccgcggtaat aaattaagca aagcgcaaga aaatgccgcc tggtttactg ctcttgcgaa
     5281 tcgcagcgtg caggtgacct gtcagacacc ggagcaggct cagcttcccc gctgggttgc
     5341 tgcgcgcgca aaacagctca acttagaact ggatgacgcg gcaaatcagg tgctctgcta
     5401 ctgttatgaa ggtaacctgc tggcgctggc tcaggcactg gagcgtttat cgctgctctg
     5461 gccagacggc aaattgacat taccgcgcgt tgaacaggcg gtgaatgatg ccgcgcattt
     5521 cacccctttt cattgggttg atgctttgtt gatgggaaaa agtaagcgcg cattgcatat
     5581 tcttcagcaa ctgcgtctgg aaggcagcga accggttatt ttgttgcgca cattacaacg
     5641 tgaactgttg ttactggtta acctgaaacg ccagtctgcc catacgccac tgcgtgcgtt
     5701 gtttgataag catcgggtat ggcagaaccg ccggggcatg atgggcgagg cgttaaatcg
     5761 cttaagtcag acgcagttac gtcaggccgt gcaactcctg acacgaacgg aactcaccct
     5821 caaacaagat tacggtcagt cagtgtgggc agagctgaaa gggttatctc ttctgttgtg
     5881 ccataaaccc ctggcggacg tatttatcga cggttgatat gaaatcttta caggctctgt
     5941 ttggcggcac ctttgatccg gtgcactatg gtcatctaaa acccgtggaa acgctggcga
     6001 atttgattgg tctgacgcgg gtcacaatca tccctaataa tgttcctccg catcgtcccc
     6061 agccggaagc gaacagcgtg cagcgtaaac acatgcttga actggcgatt gccgacaagc
     6121 cattatttac tcttgatgaa cgcgagctaa agcgcaatgc cccctcttac actgcgcaaa
     6181 cactgaaaga gtggcggcag gaacaaggac cggacgtgcc gctggcgttt attattggtc
     6241 aggattcact gctgaccttt ccgacctggt acgaatacga aacgatactc gacaatgcac
     6301 atttgatcgt ctgtcggcgt ccaggttacc cacttgaaat ggcgcaaccg caataccagc
     6361 aatggctgga agatcatttg acacataacc cggaagatct tcaccttcag cctgccggta
     6421 aaatttatct ggctgaaacg ccgtggttta acatctcggc gaccatcatc cgcgaacgtt
     6481 tgcaaaacgg tgaatcgtgt gaggatttat tgccggaacc ggtactgact tacattaacc
     6541 aacaaggctt gtatcgctga tacctgtcgt tacggagggc atgatgcgac tgtggttaat
     6601 tcgtcatggt gaaacgcaag cgaatatcga tggtctttac agcggtcatg cgcccacccc
     6661 cctgaccgcg cgcggtattg agcaagcgca aaatctgcat acgctgctac atggtgtttc
     6721 ctttgatctg gttttatgca gtgaactgga acgggcacag cataccgcac gactggttct
     6781 cagtgaccgc cagctccccg tgcaaatcat ccctgaactc aacgaaatgt tttttggcga
     6841 ctgggagatg cgacatcatc gcgacctcat gcaagaagat gccgaaaact atagcgcgtg
     6901 gtgcaatgac tggcagcatg caatccccac gaacggtgaa ggatttcagg cattttcgca
     6961 acgtgtggaa cgctttatcg caaggcttag tgaatttcag cactatcaga atattttagt
     7021 cgtcagccat cagggtgtac tgagtctgtt aatcgcccgt ttaattggca tgcctgccga
     7081 ggctatgtgg cattttcgcg ttgactaggg gtgttggagc gccattgata tcaaccaaaa
     7141 attcgcgacg ctacgcgtcc tcaatagccg tgccatcggg gtcgagaatg catgactttt
     7201 ctgttttttt acgggtaagc cgcaacgacc attgacagac ccgggcaggc tgatattctc
     7261 cgcagccaga cttttttcgc cagacacgac tttgtagaaa ttgttttaca aaaatggcga
     7321 tgcaatctgc ggcgcggggt gggatgatag cccactttcg aaagccgatt cggcgacaat
     7381 tgtcccgaaa tcgcctctgg ttcaggtata ctgacagacc atttttatct atttgattca
     7441 cccaggggga aaacttgcag ggtaaagcac tccaggattt tgttatcgac aaaattgatg
     7501 acctcaaagg tcaggacatc atcgccttag acgttcaagg caaatccagc atcaccgact
     7561 gcatgatcat ctgtacgggt acgtccagcc gtcatgttat gtccattgct gaccacgttg
     7621 tgcaggagtc tcgcgcagcg ggcctgttac cgctcggcgt agaaggtgaa aacagcgccg
     7681 actggattgt cgtggatttg ggcgatgtga ttgtccatgt catgcaggaa gagagccgtc
     7741 gcctgtatga actggaaaaa ctctggagtt aatgcgtgaa gctgcaactt gtcgccgtgg
     7801 gaacgaaaat gccggactgg gtacaaaccg gttttaccga gtacctgcgt cgttttccga
     7861 aagatatgcc cttcgagctg attgaaattc cggccggaaa acgcggcaag aatgcggaca
     7921 tcaagcgcat actcgacaaa gagggtgagc agatgttggc ggccgcaggc aaaaaccgca
     7981 ttgtcaccct cgatattcca ggcaagccct gggatacgcc gcagttagcc gctgagctgg
     8041 aacgctggaa gctggatggt cgcgacgtca gtctactgat tggcgggcct gaagggttgt
     8101 cgcctgcctg taaagcggcg gcggagcaga gctggtcgct gtcggcgctt accctccccc
     8161 atccgctggt tcgcgtgctg gtcgcagaga gtctgtaccg ggcgtggagc atcaccacca
     8221 accatcctta tcaccgtgag tgataaggga gctttgagta gaaaacgcag cggatgaaac
     8281 tacagaactc ttttcgcgac tatacggcag agtccgcgct gtttgtgcgc cgggcgctgg
     8341 tcgccttttt ggggattttg ctgctgaccg gcgtgcttat cgccaacctg tataatctgc
     8401 aaattgttcg ctttaccgac taccagactc gctctaatga aaaccgcatt aagctggtgc
     8461 ctatcgcgcc cagccgcggc attatctacg accgtaatgg tatccctctg gccctcaacc
     8521 gcactatcta ccagatagaa atgatgccag agaaagtcga taacgtgcag caaacgctgg
     8581 acgctttgcg cagcgtggtt gatctgaccg atgacgatat tgctgcattc cgaaaagagc
     8641 gcgcacgttc acaccgtttc acctctattc cggtgaaaac taacctgacc gaagtacaag
     8701 tagctcgctt tgccgtcaat cagtaccgtt ttccgggtgt cgaagttaaa ggctataaac
     8761 gtcgttacta tccttacggt tcggcgttga cccacgtcat cggctatgtg tcgaaaatca
     8821 acgataaaga cgtcgaacgc ctgaataatg acggcaaact ggccaactat gcggcaacgc
     8881 atgatatcgg taagctgggc attgagcgtt actatgaaga cgtgctgcac ggtcagaccg
     8941 gttatgaaga ggttgaagtt aacaaccgtg ggcgtgttat ccgccagtta aaagaagtac
     9001 caccgcaagc cggacacgat atttacctga cgctggatct caaactccag caatatattg
     9061 aaacgctgct ggcgggtagc cgcgcagctg tggttgtcac cgatccgcgt acaggtgggg
     9121 tgctggcgct ggtttccacg cctagttatg acccaaacct gtttgttgac ggtatctcca
     9181 gcaaagatta ttccgccttg ttgaacgccc cgaatacacc gctggtgaac cgcgccacac
     9241 agggggttta tcctcccgcg tctacagtta aaccctatgt ggcggtttcg gcattgagcg
     9301 ccggggtgat cacgcgcaat acgacgctgt ttgacccagg ctggtggcaa ctgccaggtt
     9361 cggaaaaacg ttatcgtgac tggaaaaaat ggggccacgg gcgtctgaat gtcacaagat
     9421 cgctggaaga atctgcggat accttcttct atcaggtggc ctacgatatg gggatcgatc
     9481 gcctctccga atggatgggt aaattcggtt atggtcatta caccggtatc gacctggcgg
     9541 aagaacgttc cggcaacatg cctacccgcg aatggaaaca gaaacgcttt aaaaaaccgt
     9601 ggtatcaggg tgacaccatt ccggttggta tcggtcaggg ttactggaca gcgaccccaa
     9661 tccagatgag taaggcactg atgatcctga ttaatgacgg tatcgtgaag gttcctcatt
     9721 tgctgatgag caccgccgaa gacggcaaac aggtgccatg ggtacagccg catgaaccgc
     9781 ccgtcggcga tattcattcc ggttactggg agctggcgaa agacggtatg tacggtgttg
     9841 ctaaccgccc taacggtacg gcgcataaat actttgccag cgcaccgtac aaaattgcgg
     9901 cgaaatccgg taccgctcag gtcttcggtc tgaaagcgaa cgaaacctat aatgcgcaca
     9961 aaattgccga gcgtttacgt gaccacaaac tgatgaccgc ctttgcgcca tacaacaatc
    10021 cgcaagtggc tgtcgccatg attctggaga acggtggtgc gggtccggcg gttggtacac
    10081 tgatgcgcca gatcctcgac cacattatgc tgggtgataa caacaccgat ctgcctgcgg
    10141 aaaatccagc ggttgccgca gcggaggacc attaatcatg acggataatc cgaataaaaa
    10201 aacattctgg gataaagtcc atctcgatcc cacaatgctg ctgatcttac tggcattgct
    10261 ggtttacagc gccctggtta tctggagcgc cagcggtcag gatattggca tgatggagcg
    10321 taaaatcggc caaatcgcga tgggtctagt catcatggtg gtgatggcgc aaattcctcc
    10381 acgcgtttat gaaggctggg ccccctatct ctatatcatc tgtattattt tgctggtggc
    10441 ggtagatgct ttcggtgcca tctctaaagg tgctcaacgc tggctggacc tcggtattgt
    10501 tcgttttcag ccgtcggaaa ttgccaaaat agccgtacca ctgatggttg cgcgctttat
    10561 caaccgcgac gtttgcccgc catcgttgaa gaacactggc atcgcgctgg tgctgatatt
    10621 tatgcccacg ctgctggtgg ctgcacagcc tgacctggga acatcaatcc tcgttgcgct
    10681 ttccggtctg tttgtactgt tcctctctgg ccttagctgg cgtctgattg gcgtcgcagt
    10741 agtgctggtg gcggcgttca ttccgattct gtggttcttc ctgatgcatg attaccagcg
    10801 ccagcgcgta atgatgctcc tagacccgga atcagaccca ctcggcgcgg gctatcacat
    10861 tattcagtct aaaattgcta ttggctccgg cggattacgc ggcaaaggct ggctgcacgg
    10921 cactcagtca cagcttgaat ttctccccga acgccatacc gactttatct tcgcggtact
    10981 ggcggaagag ctgggattag tgggcattct gattctgctc gctctctaca ttctgctgat
    11041 catgcgcggg ctgtggatag ccgccagagc gcaaaccacc tttggtcgcg tcatggctgg
    11101 tggcttaatg ctgatattat tcgtttatgt cttcgtaaat attggtatgg taagcggtat
    11161 tctgccggtt gtaggggttc cgctcccact ggtcagttat ggaggatcgg cgctaattgt
    11221 gctgatggct gggttcggga ttgtaatgtc aatccacacc cacaggaaaa tgttgtcgaa
    11281 aagcgtgtaa gaggtgcgca atgcgtaagc agtggctcgg gatctgcatc gcggcaggaa
    11341 tgctcgcggc atgtacaagc gatgatggtc agcaacagac ggtaagtgta ccgcagcctg
    11401 cggtatgtaa cggccccata gttgaaatta gcggggcgga cccgcgtttc gaaccactga
    11461 acgcgacggc aaatcaggat taccagcgcg acggtaaaag ctacaaaatc gtgcaggatc
    11521 cgtctcgatt tagccaggcg ggactggcgg caatctatga tgccgaaccc ggcagtaacc
    11581 tgacggcctc tggcgaagct ttcgatccga cacagctgac ggcggcccat ccaacgcttc
    11641 cgatccccag ctacgccaga atcactaacc tggctaacgg gcgaatgatc gtggtgcgca
    11701 ttaatgatcg cggcccttac ggcaacgacc gcgttatttc actttctcgc gcggcagctg
    11761 accgtcttaa cacgtcaaac aacaccaaag ttcgtatcga tccgattatt gtcgcccagg
    11821 atggttcgct ttctggtcct ggtatggctt gtaccacagt cgccaaacag acttacgccc
    11881 tgccagcacc tcccgattta agcggtggcg cgggaacaag ttcagtgtct ggcccgcagg
    11941 gtgacattct tccggtcagt aattcgacgc taaaaagcga agatccgacc ggcgcgccgg
    12001 taaccagtag cggtttcctc ggcgctccaa cgaccttagc gcctggtgta ctggaaggca
    12061 gcgaaccgac gcctgcgcca cagcccgttg ttacagctcc gtcgacaacg cctgcaacct
    12121 cgcctgcaat ggtgacaccg caagccgcct cgcaaagcgc cagcggcaac tttatggtgc
    12181 aagtcggggc cgtaagcgat caggctcgtg cgcaacagta ccaacagcaa ctgggacaga
    12241 agttcggcgt ccccggtcgc gtaactcaaa atggcgcggt ctggcggatc cagcttggcc
    12301 cattcgccag caaagccgaa gccagtacct tgcagcaacg tttgcaaacc gaagcccaat
    12361 tacagtcatt tattactacc gcgcagtagc gtaaagcagg catctgatgt gtcaatttct
    12421 gtaaatgacg ttaacaaagt cacgcggaaa gtcagatgcc tgccggtagt gcatttgcta
    12481 tagtagggca cttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    12541 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    12601 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    12661 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    12721 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    12781 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    12841 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    12901 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg
    12961 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    13021 acgttaacgc actgggcctg aaaaataccc acttccagac ggtacatggt ctggatgctg
    13081 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
    13141 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    13201 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    13261 acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga
    13321 tttctgcggt gatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    13381 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    13441 cctctgaacc ggtttggttt ggtgattctg atcgcgcttc gttaggggtt gataaagacg
    13501 tgtacctgac cattccacgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    13561 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    13621 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
    13681 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    13741 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaactcc
    13801 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    13861 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    13921 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    13981 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    14041 actcatatcg agcaggttga aacactgtat gaagaactag gcaaaatcga tattgtccgc
    14101 atggttctgt aactcgcttc tccgttaccc ggcccctggt cgggtaactc ccctctcctc
    14161 gctgtgatat acttttcccc cacttttact cattctccac ggagatgcag ttttgtatca
    14221 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat
    14281 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    14341 ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa tgccgggtga
    14401 tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc cggggcaaca
    14461 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    14521 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    14581 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgtat
    14641 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    14701 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa
    14761 acccgaagcg acgactaata atattgcacc acgtttactg gaaaatattt tagcgctact
    14821 aaacaatcct gacttcgaat atattaccgc ttaattgcat acctcaatgg cccaatttac
    14881 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatactt
    14941 tctttatcac ttgcagaaat gcggtgctac gccttgctcc gtaactcacc ccattgatta
    15001 acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta gacataagat
    15061 tattgtaatt tttttaaata ttatttttcc atgaataaaa ttggagtaag tgcgtggata
    15121 gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    15181 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    15241 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt
    15301 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    15361 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    15421 ttcttggcgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    15481 ccactccctc ggtcggagcc ctggtccttc ctgtcatcta tcgggcgatt aaaactcact
    15541 atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt
    15601 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    15661 ttctgttcac cgacaatatg gtgttaattt gccgcgaggg aaatccacta ctctctttag
    15721 aagatgacag agagactatc gacaacgctg cgcatgtact cctgttaccg gaagggcaaa
    15781 atttcagcgg tctgcggcag agagttcaag agatgtttcc ggaccggcaa atcaatttca
    15841 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    15901 ttcccagccg tttttataac ctgtttagtc gttgctggcc gctggaaaaa ctgccttttc
    15961 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc agcctgcgtg
    16021 atcccatcct gcacggggta atagatgtta tccgtaatgc attttgaaaa agtattgtta
    16081 cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
    16141 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    16201 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    16261 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    16321 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    16381 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    16441 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    16501 ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
    16561 cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
    16621 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    16681 gttgaccgtg atgacctgcg cgatggcggt gcccagcact ttgcggattg cattactgcc
    16741 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgtggtcgt
    16801 atggatcgtg ctctggatat tctgactgca acgccaccag atgtgttcaa ccataacctg
    16861 gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg cagattacaa ctggtcgctg
    16921 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    16981 gtgggactgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    17041 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    17101 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    17161 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
    17221 aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    17281 gctttttg
//
LOCUS       DAZOZY010000077        16905 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZOZY010000077 DAZOZY010000000
VERSION     DAZOZY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16905
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.077"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            284..1336
                     /locus_tag="ACHURQ_002215"
     CDS             284..1336
                     /locus_tag="ACHURQ_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR0823979.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1652..2854)
                     /locus_tag="ACHURQ_002216"
     CDS             complement(1652..2854)
                     /locus_tag="ACHURQ_002216"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR0823980.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3086..4585
                     /gene="pitA"
                     /locus_tag="ACHURQ_002217"
     CDS             3086..4585
                     /gene="pitA"
                     /locus_tag="ACHURQ_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR0823981.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4829..5164)
                     /gene="uspB"
                     /locus_tag="ACHURQ_002218"
     CDS             complement(4829..5164)
                     /gene="uspB"
                     /locus_tag="ACHURQ_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR0823982.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5555..5989
                     /gene="uspA"
                     /locus_tag="ACHURQ_002219"
     CDS             5555..5989
                     /gene="uspA"
                     /locus_tag="ACHURQ_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR0823983.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(6088..6141)
                     /locus_tag="ACHURQ_002220"
     CDS             complement(6088..6141)
                     /locus_tag="ACHURQ_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823984.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6307..7776
                     /gene="dtpB"
                     /locus_tag="ACHURQ_002221"
     CDS             6307..7776
                     /gene="dtpB"
                     /locus_tag="ACHURQ_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR0823985.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7825..8577)
                     /gene="rsmJ"
                     /locus_tag="ACHURQ_002222"
     CDS             complement(7825..8577)
                     /gene="rsmJ"
                     /locus_tag="ACHURQ_002222"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR0823986.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8585..10627)
                     /gene="prlC"
                     /locus_tag="ACHURQ_002223"
     CDS             complement(8585..10627)
                     /gene="prlC"
                     /locus_tag="ACHURQ_002223"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR0823987.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10830..11672
                     /gene="rlmJ"
                     /locus_tag="ACHURQ_002224"
     CDS             10830..11672
                     /gene="rlmJ"
                     /locus_tag="ACHURQ_002224"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR0823988.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11744..13096
                     /gene="gorA"
                     /locus_tag="ACHURQ_002225"
     CDS             11744..13096
                     /gene="gorA"
                     /locus_tag="ACHURQ_002225"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR0823989.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13150..13233)
                     /gene="dinQ"
                     /locus_tag="ACHURQ_002226"
     CDS             complement(13150..13233)
                     /gene="dinQ"
                     /locus_tag="ACHURQ_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR0823990.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13261..13434
                     /locus_tag="ACHURQ_002227"
     CDS             13261..13434
                     /locus_tag="ACHURQ_002227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0823991.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            13973..14326
                     /gene="arsR"
                     /locus_tag="ACHURQ_002228"
     CDS             13973..14326
                     /gene="arsR"
                     /locus_tag="ACHURQ_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR0823992.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14380..15669
                     /gene="arsB"
                     /locus_tag="ACHURQ_002229"
     CDS             14380..15669
                     /gene="arsB"
                     /locus_tag="ACHURQ_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR0823993.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15682..16107
                     /gene="arsC"
                     /locus_tag="ACHURQ_002230"
     CDS             15682..16107
                     /gene="arsC"
                     /locus_tag="ACHURQ_002230"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR0823994.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16241..16537)
                     /locus_tag="ACHURQ_002231"
     CDS             complement(16241..16537)
                     /locus_tag="ACHURQ_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0823995.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            16644..>16905
                     /locus_tag="ACHURQ_002232"
     CDS             16644..>16905
                     /locus_tag="ACHURQ_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0823996.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         3899 a         4386 c         4285 g         4335 t
ORIGIN      
        1 atgattttaa gcaccgagac tatccggtct tttactaaat aatggaaaac aatccatccc
       61 tggaagataa ttgtaaaaaa aacaacagaa taagaattaa tcttaggata aatatttatt
      121 tatcatggcc ttttaaccgg taaaaaatgg cactcggcac agtaaacccc gctatagtta
      181 tgctatgcat ttgtctttaa tgatatctcg tcgttatatt aacaggctga aattatcatt
      241 aatgcacatt tcattattag cagggatacc tgaggagttt aaagtgaaca tatatatcgg
      301 gtggcttttc aaattaatcc ctttgattat gggattaatt tgcatcgcgt taggtggctt
      361 tgtgctggaa agttcagggc aaagcgagta tttcgtcgcg ggtcatgtgc tgatttctct
      421 ggcggccata tgcctggcat tattcactac cgcatttatt atcatttcgc agctcacgcg
      481 cggcgttaat acgttttaca atacattgtt ccccattatt ggctatgcgg ggtcaattat
      541 caccatgata tggggttggg cactgttagc aggcaatgat gtgatggcag acgagtttgt
      601 cgccggccat gttattttcg gcgttggtat gattgccgcc tgtgtatcga cggtggcagc
      661 gtcatccggt cactttctgc tcattcccaa aaatgcagcg gggagcaaga gcgatggaac
      721 gccggtacag gcttattctt cattaatcgg taattgcctc attgccgttc ccgttttact
      781 caccctgctc ggtttcattt ggtctattac gctgttacgt agtgctgaca taactccgca
      841 ttatgtcgcg ggtcacgtat tgcttgggtt aaccgcaatc tgtgcctgtc taattggcct
      901 tgttgccaca attgtccatc aaacacgtaa tacgttttca actaaagaac actggctgtg
      961 gtgttattgg gttatttttc tcggctcaat cacggtactg caggggatat acgtcttagt
     1021 cagttccgat gcaagcgccc gactggctcc cggcattatt cttatttgcc tcggaatgat
     1081 ctgttacagc atattctcaa aagtctggct actggcactg gtctggagac gtacctgttc
     1141 gttagccaac agaataccga tgatacccgt cttcacctgc ctgttttgcc ttttcctggc
     1201 atcatttctt gcggaaatgg cgcagaccga catgggatat tttattcctt cgcgagttct
     1261 ggtcggtttg ggagcggtat gctttacgtt gttctcaatc gtttcaatat tagaagcggg
     1321 ttctgctaaa aaataattgc aacgtaccgg ataaaaccag cgttgaccat ttgcgtaacg
     1381 ctggtttttc ttaggcatca tgaaataacg cagcattaat gcatagtggt taaggataaa
     1441 aaaacaacaa agttactgtc tttttcagcc tgttcatatt ttataaagat ctggccactg
     1501 ggtgagtttt caatcacctt tccttccacc ttatattaag cctggagggt ttcagttcac
     1561 gggctcatta gaaaataatc acaagatagc cactatcaaa attagctcat ttttaatgcg
     1621 gctctaataa ttttcatctt taggtaacag gtcaggacga cttcgctgca atcaaatcct
     1681 gcgcacaagc ccacgcactc gaccacgccc actggaagtt atagcccccc agccagccgg
     1741 taacgtccat cacttcgccg atgaagtaca gcccaggcac tttgcgcgct tccatcgtcc
     1801 gtgaagagag ttcgttggtg tccacgccgc cgagcgtcac ttcggcagtg cgatagcctt
     1861 cagtgccgtt gggttgtacg cgccagtcgg tcaatgtgct aatcagtgcc tgttggtcac
     1921 gcacgttgag ctgttttagc gaaacattcg ggatttgccc gagttgctgt aagcgttcaa
     1981 ccaaccgctt cggtagatga accgccagtg tgtttttcag gctctgattc ggatgtgcgt
     2041 tacgctgctc attcaggaag gtttcgagat ccacatccgg tagcagattg atactgacaa
     2101 attcacccgg ttgccagtag ctggaaatct gcaacaccgc cggtccagac aagccgcgat
     2161 gggtgaagag taagttctca cggaaaacga tgccgttttc agcggtaatc acggaaggca
     2221 ccgccacgcc cgccagcacc tgtaactctt cgagcaacgg tttatgcaga gtgaatggca
     2281 ccagacccgc acgggtcggc agcacgttga ggccaaattg ttcggcaatc ttataaccaa
     2341 acggcgacgc gcccagcccc ggcattgaca gcccaccggt cgccatgacc agcttttcgc
     2401 aaccgacagt catgccgttc agatcaagcg tgaagcctgt ttcatcctcc gccacactca
     2461 gcacttcgct acgcaatctg aaggtcacat tgcccttctc gcactcatcc accagcatgt
     2521 cgacaatctg ctgcgcggag tcatcgcaga agagttgccc taacgttttc tcgtgccatg
     2581 cgatgccgtg tttattgacc agatcaatga aatcccactg ggtaaaacgt gcgagtgcag
     2641 acttacaaaa atgcggattc tggctcagat aagcgcctgg ttcgacataa aggttggtaa
     2701 agttgcagcg cccaccgcca gacataagga ttttgcgccc tggtttttta ccattatcga
     2761 tcagcagaac ccggcgtcct gcctgacctg ccagcgcaga acagaacata cccgccgcac
     2821 cagcgcctat aataatggca tcaaaccttt ccacgttgcg ctcctcttag aaaaaacggg
     2881 cgtgaattgt aaagattcct cagtggtcgc accagcatca atattactaa aaggaagtat
     2941 ttgcctgaat tatataagat aattattttt tgagtgaaat ccatacagga ggcaaatcaa
     3001 aaaaagtcta tatttcactt tgcccgcgcc gcgaaagtca ctgataatgc gccgcgttca
     3061 tgtcctcaaa atggcgtaac gtcctatgct acatttgttt gctggcctgg atttgcatac
     3121 cgggctgtta ttattgcttg cactggcttt tgtgctgttc tacgaagcca tcaatggttt
     3181 ccatgacaca gccaacgccg tggcaaccgt tatctatacc cgcgcgatgc gttctcagct
     3241 cgccgtggtt atggcggcgg tgttcaactt tttgggtgtt ttgctgggtg gtctgagtgt
     3301 tgcctatgcc attgtgcata tgctgccgac ggatctgctg cttaatatgg gatcgtctca
     3361 tggccttgcc atggtgttct ctatgttgct ggcggcgatt atctggaacc tgggtacctg
     3421 gtactttggt ttacctgcat ccagctctca tacgctgatt ggcgcgatca tcgggattgg
     3481 tttaaccaat gcgttgatga ccgggacgtc agtggtggat gcactcaata tcccgaaagt
     3541 attaagtatt ttcggttctc tgatcgtttc ccctattgtc ggcctggtgt ttgctggcgg
     3601 tctgattttc ttgctgcgtc gctactggag cggcaccaag aaacgcgccc gtatccacct
     3661 gaccccagcg gagcgtgaaa agaaagacgg caagaaaaag ccgccgttct ggacacgtat
     3721 cgcgctgatc ctttccgcta tcggcgtggc gttttcgcac ggcgcgaacg atggtcagaa
     3781 aggcattggt ctggttatgt tggtattgat tggcgtcgcg ccagcaggct tcgtggtgaa
     3841 catgaatgcc actggctacg aaatcacccg tacccgtgat gccatcaaca acgtcgaagc
     3901 ttactttgag cagcatcctg cgctgctcaa acaggctacc ggtgctgatc agttagtacc
     3961 ggctccggaa gctggcgcaa cgcaacctgc ggagttccac tgccatccgt cgaataccat
     4021 taacgcgctc aaccgcctga aaggtatgtt gaccaccgat gtggaaagct acgacaagct
     4081 gtcgcttgat caacgtagcc agatgcgccg cattatgctg tgcgtttctg acactatcga
     4141 caaagtggtg aagatgcctg gcgtgagtgc tgacgatcag cgcctgttga agaaactgaa
     4201 gtccgacatg cttagcacca tcgagtatgc accggtgtgg atcatcatgg cggtcgcgct
     4261 ggcgttaggt atcggtacga tgattggctg gcgccgtgtg gcaacgacta tcggtgagaa
     4321 aatcggtaag aaaggcatga cctacgctca ggggatgtct gcccagatga cggcggcagt
     4381 gtctatcggc ctggcgagtt ataccgggat gccggtttcc actactcacg tactctcctc
     4441 ttctgtcgcg gggacgatgg tggtagatgg cggcggctta cagcgtaaaa ccgtaaccag
     4501 tattctgatg gcctgggtgt ttacccttcc ggctgcggta ctgctttccg gcgggctgta
     4561 ctggctctcc ttgcagttcc tgtaatcgta cgcaccaaaa cgagcgggtc agctggcccg
     4621 cttcagattg tgacatagtg cgctttgttc atgccggatg cggcgtgaac gccttatccg
     4681 gcctacaaaa acatgcaaat tcaatagatt gcagagatta tgtaggcctg ataagcgtag
     4741 cgcatcaggc aattttgcgt ttgtaatcag tctcgagcgg gtcagtttac tggcccgctt
     4801 tttttatgcg ttattattgc tgaagtgctt aatgccaaat catcaatgca atcaggctga
     4861 ccaccaccag accacacaat gcgctggtca gaataaactg ccgacgcacc cgctcacagc
     4921 ggcgaataaa ttcatcatcg tgatgatcgc gataacgttg ggcatagata taccaaacga
     4981 gacgcacctg tttgttgggt tggccatgtg aggtaaaaaa gccccctcca tcaacatatt
     5041 gatagagcaa tggatcgcag ttacgcagta ccactaacaa cgcgcgtagt gatgagaaat
     5101 agcgcgccat gttaacaatg caaacgacac ataaagccca aaataatgcg acggtgctta
     5161 tcatacctcc tccccggcga cctgcccgcg gagttccacc ccggggctac cgctcccgat
     5221 acgctgccaa tcagttaaca ccaggtcctg gagaaaccgc ttttgtggtg accaacatcc
     5281 gagcggctct atagatagtg taggagatca ggttgttttt tttccagaag gttaaccact
     5341 atcaatatat tcatgtcgaa aatttgttta tctaacgggt aagcaaggcg gattgacgga
     5401 tcatccgggt cgctataagg taaggatggt cttaacactg aatctttacg gctgggttag
     5461 ccccgcgcac gtagttcgca ggacgcgggt gacgtaacgg cacaagaaac gctagctggc
     5521 cagtcatcga caactttatg gaaggagtaa cactatggct tataaacaca ttctcatcgc
     5581 ggtcgatctc tccccggaaa gcaaagttct ggtagagaaa gcagtctcta tggctcgccc
     5641 ctacaatgcg aaagtttctc tgatccacgt agatgtaaac tactctgacc tatacaccgg
     5701 gcttattgat gtgaatctgg gtgatatgca gaaacgcatc tctgaagaga cacatcatgc
     5761 gctgaccgag ctttccacta atgcaggcta cccaatcact gaaaccctga gcggcagcgg
     5821 cgacctgggc caggttctgg tcgatgcaat caagaaatac gatatggatc tggtggtttg
     5881 tggtcaccac caggacttct ggagcaaact aatgtcttcc gcacgtcagc tgatcaacac
     5941 cgttcacgtt gatatgctga ttgttccgct gcgcgacgaa gaagaataat catcccccca
     6001 cgacgtgttc ctgaacgccc gcatatgcgg gcgttttgct ttttggcgcg ccttgttacc
     6061 tgatgcagcg taaacccctt atctggccta cgatctgcgt acgcaatcaa aatctcctgc
     6121 caatacaaca tttaacacta tcatattttc catcattagt gtgatcagct ggttattttc
     6181 tgttgtaata gtgtattaat ctattcaccg catcaatatt aagaatctct gacagatgta
     6241 aactttttcg cgcgttatcc cttacgcgtt catacttttc aggatggtat tggaaggtta
     6301 ataaatatga atacaacaac acccatgggg atgctgcagc aacctcgccc atttttcatg
     6361 atcttttttg tcgagttatg ggagcgattc ggctactacg gcgtgcaggg cgtactggcg
     6421 gttttcttcg ttaaacagct tggattctcg caagagcagg cttttgtcac ttttggtgct
     6481 tttgctgcgc tggtctatgg cctcatttcc attggcggct atgtcggcga ccacctgctg
     6541 gggaccaaac gcaccattgt tctcggagca cttgtgctgg cgattggcta cttcatgacc
     6601 ggcatgtcgc tacttaagcc tgacctgatt ttcatcgccc tggggactat cgctgtcggt
     6661 aacggcctgt ttaaagctaa cccagccagc ttgctttcga agtgctatcc gccgaaagat
     6721 ccgcggcttg atggcgcatt caccctgttc tatatgtcga tcaatatcgg ctcgttgata
     6781 gcgttatcgc tggcccctgt gatcgctgat agatacggtt attcagtcac ctacaacctg
     6841 tgcggggcgg ggttaattat cgcattactg gtttacatcg cctgtcgtgg aatggtgaaa
     6901 gacattggtt ctgaacccga cttcaagccg atgagcttca gcaaactgtt gtacgtatta
     6961 cttggcagcg tggtgatgat cttcgtatgt gcatggctga tgcacaacgt agaagtcgcc
     7021 aatctggtgc tgattgttct ctccatcgtc gtcaccatca tcttctttcg tcaggcattc
     7081 aagctggata aaaccgggcg caataaaatg tttgtcgcct ttgtcctgat gctcgaagcg
     7141 gtggtgtttt acattctcta cgcccagatg ccaacgtcgc tgaacttctt tgccatcaac
     7201 aacgtgcatc atgaaattct cggcttttcc ataaacccgg tcagcttcca ggcgcttaac
     7261 ccgttctggg tggtactcgc cagcccaata ctggcaggca tttacacgca tctgggtaac
     7321 aaaggcaaag acctctcgat gccgatgaaa tttactctcg gcatgtttat gtgctcactg
     7381 ggctttttga cggcggcagc tgcgggaatg tggtttgcgg atgcacaagg gctgacatcg
     7441 ccatggttta tcgtgctggt gtatttattc cagagcctgg gtgaattgtt tattagcgcc
     7501 cttggcctgg cgatgattgc tgccctggtg ccgcagcatt tgatgggctt tattctcggg
     7561 atgtggttcc tgacgcaggc tgccgcattc ttgctgggcg gctatgtggc aacatttacc
     7621 gcagtgccgg acaacattac cgatccgctt gagacgttac ccgtctatac caacgtgttt
     7681 ggtaagattg gcctggttac gctgggcgtt gcagtagtga tgctgttgat ggtgccgtgg
     7741 ctgaaacgca tgattgcgac gccggaaagc cattaattat tcttgcagaa agcagggtag
     7801 cgttatcgct accctgcttt acttttacac gggcgtacct gcatagatat caaagcgatg
     7861 ccctttagtc acaaccgcgt ttggcgtggc gacattcgct agcggtggcg cgtagtccgg
     7921 gcgctttact accacgcgtt tggtcgccag caagcgtgca ggctccagca atccatcggc
     7981 atcaagatcc ggccccacca gcgactgaaa gacacgcatc tcttttttca ccagcgcgct
     8041 tttctgctta tgcgggaaca tcgggtcgag atagaccacc tgcgggcgtg gggtaatatc
     8101 ggttagcgcc gtcaggctgg aggcgtgaat taactgcaaa cgctcctgca accagccgcc
     8161 gatttccgca tccgcataac cacgcgccag gccgtcgtcg agcagcgcgg caacaactgg
     8221 attacgctcc agcatccgca cgcggcaacc gactgaagcc agcacaaagg catcgcgccc
     8281 cagtcctgca gtggcatcca ccacatccgg caaataatcg cctttaatgc ccaccgcttt
     8341 cgccaccgcc tcaccgcgac caccgccgaa tttgcgtcgg tgcgccatcg ctccgccaac
     8401 aaaatcaaca aagatgccgc caagttttgg ctcatcacgc ttgcgcaatt ccagatgttc
     8461 cggcgttaac accagcgcca tcaggttgtc ttcatcgtgc tccagccccc agcgggccgc
     8521 cagaacagat aaggcgccgt ctccggcgcc tgtttcatca attaagcaga ttttcactga
     8581 atgatcagcc cttaatgccg taatgctcca gcatcgcatc cagctgcggt tcacgaccac
     8641 ggaagcgttt gaacagatcc atcggctctt ctgaaccgcc acggctcaga atgttgtcga
     8701 ggaacgactg cccggtttca cggttgaaaa tgccctcttc ctcaaagcgc gagaaagcat
     8761 ctgccgccag tacgtcagcc cacaggtagc tgtagtaacc tgccgcataa ccaccggcga
     8821 aaatatggct gaaagcgtgc gggaaacggc cccaggacgg agatggcacc acggcaacca
     8881 gtttcttgat ttctgccaga gtttcgagga tttttgcccc ctgatccggg cggaactcgg
     8941 catgaaggcg gaaatcaaac aggccgaact ccagctgacg cagaataaac agcgccgcct
     9001 ggtagttctt cgccgccagc attttatcca gcaactcttt cggcagcggt tcgccggttt
     9061 catagtgacc agagataaac gccagcgcct ccggctccca gcaccagttt tccataaact
     9121 gactcggcag ttcgaccgca tcccacggca caccgctgat accggaaaca ccagcggttt
     9181 cgatgcgggt cagcatatgg tgcaggccgt gaccgaactc gtggaacagg gtgatcactt
     9241 cgtcgtgagt gaacagcgcc ggtttaccat ttaccgggcg gttgaagtta caagtcaaat
     9301 acgcgaccgg tttttgcaga gaaccatcag ctttacgcat ctggcctacg cagtcatcca
     9361 tccacgcccc gccgcgcttg ttttcacggg catacagatc gaggtagaag ctgccgcgca
     9421 gttcgttatt ttcgtcatac agttcgaaga aacgtacatc cggatgccag acatcaacct
     9481 ctttacgctc tttagcggtg atgccgtaaa tacgtttcac cacttcaaac aggccgttaa
     9541 ccgctttgtt ttccgggaag tacggacgca gctgctcgtc gctgatgctg tagaggtgct
     9601 gtttctgttt ttcgctgtag taagcgatat cccacggctg caactcatcg acaccaaatt
     9661 cggctttagc gaaggcacgc agttgctcca gctctttttc gccttgcgga cgcgcgcgtt
     9721 ttgccagatc ggttaagaaa tccagcacct gctgcgggtt ttctgccatt ttagtggcaa
     9781 gggatttaaa ggcgtagttt tcaaagccca gcagttgcgc cagttcgtga cgcagcgcga
     9841 ggatctcttc catcaccttg ctgttatccc acttaccggc gttcgggcct tgatcggagg
     9901 cgcgggtgct gtaagcgcga tacatctctt cgcgcagagc ctggttgtcg cagtaggtca
     9961 ttaccggcag gtagcttggg atatccagcg tcagcaggta gccttccagc tctttcgctt
    10021 cggcctgggc ttttgccgca gccagcgcgc tttctggcat ccccgccagc cccgcttcgt
    10081 cggtaacgag tttggtccag cccatcgtcg catcgaggac gttgttgctg tactggttgc
    10141 ccagttcaga aagacgggta gcaatttcgc cgtaacgctg ctgtttctct ttcggcagac
    10201 ctatgccaga gagttcaaaa tcacgcaggg cgttatcaac cgctttcttc tgcgccgtgt
    10261 tcagcgtggc gtaatgatcg ccatcgcgca ggtcgcggta cgctttatac agcccttcat
    10321 gttgccctac ccaggtgctg tattccgaca gcagcggcag ggtttgctcg taggcttcac
    10381 gcagttccgg gctatttttc accgagttca ggtggctgac cggggagaag atacgcccca
    10441 acacatcgtc cacttccgcc aacggctggc agagattttc ccaggtgtac ggtgcccctt
    10501 gcgctactac gcgcgccaca ttttcgcggc aatcgttcag cgcctgagtc acggctggaa
    10561 cgacatgttc cgggagaatt ttagaaaacg gaggcaattc aaagggagtc agtaacggat
    10621 tcgtcattcg cgcagtcctg gttaaagagg ttaaggaagc gctcaacagg cgctttacat
    10681 aatgtgtgta gcatggggtt aagtgtagtg aatttcaatg agaaacgtta cgctttcgcg
    10741 gcggcggcct cttttcggta tactgtcctg atacgctttt gtgcgccccg aatacgggcc
    10801 gatttttact taccggaaca cctttaccca tgctcagtta tcgccacagc tttcacgctg
    10861 gcaaccacgc cgacgtcctt aaacataccg ttcagagcct gatcatcgag tcgctgaaag
    10921 agaaagataa accgtttctc tatctcgata cccacgcagg ggccgggcgt tatcagttag
    10981 gcagcgaaca tgccgagcgt accggcgaat atctcgaagg tatcgcccgt atctggcagc
    11041 aggacgattt gcccgcagaa ctggaggcgt acatcaatgt ggtaaaacac ttcaaccgta
    11101 gcggtcagtt gcgttactac cccgggtcgc cgttgattgc tcgccagcta ctgcgcgaac
    11161 aagacagcct gcaactgacc gaactgcacc cgagcgatta cccgttgctg cgttctgaat
    11221 ttcagaaaga cagccgtgcg cgcgtcgaaa aagccgacgg tttccagcag cttaaggcca
    11281 aacttccgcc ggtttcccgc cgtggtttaa tccttatcga cccgccgtat gaaatgaaaa
    11341 ccgactatca ggcggtggtc agcgggatag cagaaggtta caaacgtttc gccactggta
    11401 cttacgcact gtggtatccg gtggtgctgc gtcagcaaat taagcgcatg atccacgatc
    11461 tggaagcgac cggtattcgc aaaattctgc aaattgaact ggcggtactg ccagacagcg
    11521 atcgccgtgg catgaccgct tccggcatga ttgtgattaa cccgccgtgg aaactggaac
    11581 agcagatgaa taacgtgctg ccgtggctgc acagcaaact ggttccggca ggcaccgggc
    11641 acgccaccgt aagctggatc gtgccggagt aattgcagcc attgctggca cctattacgt
    11701 ctcgcgctac aatcgcggta atcaacgata aggacactct gtcatgacta aacactatga
    11761 ttacatcgcc atcggcggcg gcagcggcgg tatcgcctcc atcaaccgcg cggctatgta
    11821 cggccagaaa tgcgcactga ttgaagccaa agagctgggc ggcacctgcg tgaacgtcgg
    11881 ctgcgtgccg aaaaaagtga tgtggcacgc ggcgcaaatc cgtgaagcga tccatatgta
    11941 cggccctgat tatggtttcg ataccaccat caacaagttc aactgggaaa cgttgatcgc
    12001 cagccgtacc gcctatatcg accgtattca tacttcctat gaaaacgtgc tcggtaaaaa
    12061 taacgttgat gtaatcaaag gctttgcccg cttcgttgat gccaaaacgc tggaggtaaa
    12121 cggcgaaacc atcacggccg atcatattct gatcgccaca ggcggtcgtc cgagccaccc
    12181 ggatattccg ggcgtggaat acggtattga ttctgatggc ttcttcgccc ttcctgcttt
    12241 gccagagcgc gtggcggttg ttggcgcggg ttacatcgcc gttgagctgg cgggcgtgat
    12301 taacggcctc ggcgcgaaaa cgcatctgtt tgtgcgtaaa catgcgccgc tgcgcagctt
    12361 cgacccgatg atttccgaaa cgctggtcga agtgatgaac gccgaaggcc cgcagctgca
    12421 caccaacgcc atcccgaaag cggtagtgaa aaatgccgat ggtagcctga cgctggagct
    12481 ggaagatggt cgcagtgaaa cggtggattg cctgatttgg gcgattggtc gcgagcctgc
    12541 caatgacaac atcaacctag aagccgctgg cgttaaaact aacgaaaaag gctatatcgt
    12601 cgtcgataaa tatcaaaaca ccaatattga aggtatttac gcggtgggcg ataacacggg
    12661 tgcagtggaa ctgacaccgg tagcagttgc agcgggtcgc cgtctctctg aacgcctgtt
    12721 taataacaag ccggatgagc atctggatta cagcaacatt ccgaccgtgg tcttcagcca
    12781 tccgccgatt ggtactgttg gtttaacgga accgcaggcg cgcgagcagt acggcgacga
    12841 tcaggtgaaa gtgtataaat cctctttcac cgcgatgtat accgccgtca ccactcaccg
    12901 ccagccgtgc cgcatgaagc tggtgtgcgt tgggccagaa gagaagattg tcggtattca
    12961 cggcattggc tttggtatgg acgaaatgtt gcagggcttc gcggtggcgc tgaagatggg
    13021 cgcaaccaaa aaagacttcg acaataccgt cgccattcac ccaacggcgg cagaagagtt
    13081 cgtgaccatg cgctaaatgt taaagggcta agagtagtgt gctcttagcc cttaattacg
    13141 tttccgctat cagttcagaa gctgaagcag aaagcggatc agttccagca gcgcaattaa
    13201 cgctccaaga acgatgattg ctttatcaat cacccgtttt ctccatgcga tggagtgaga
    13261 atgcatccgc ttactcatcc actgcctgtc acggcgcatg tctcattgtt agatgacgac
    13321 tatctcactc cggccagagc atcagttaac ggcaccaccc gtacttctga ccaggacttt
    13381 gaaagcgttt atgcgcatta ccagagtgaa aatgcctcag agctaactgg ataatcatac
    13441 agtacattca ggttataaaa ccagcatgtc cttgcaatag tttcagtatg gtattagcat
    13501 tgatgcgtta gatgatgact atctcactcc agccagagcc accaactcag ggctgaagag
    13561 taaaaaaacc gacgcaaagt cggttttttt acatccggat tcggacaagg cttaatatga
    13621 cgatgaccca gtgaaagtat ataaatcgtc attgcgatat ataccgaagt gccccctccg
    13681 ccagctgaag aaatcgctaa ttcttgcaat gttagccact ggctaatagt attgaaccgt
    13741 tagatgtggg cattctcact ccagccagag ccaccaactc aggctgaaga gtaaaaaacc
    13801 gacgcaaagt cggttttttt acgtcctgat tcaaaccgcc tttcaaatga atagccaact
    13861 caaaattcac acctattacc ttcctctgca cttacacatt cgttaagtca tatatgtttt
    13921 tgacttatcc gcttcgaaga gagacactac ctgccacaat caggagcgca atatgtcatt
    13981 tctgttaccc atccaattgt tcaaaattct tgctgatgaa acccgtctgg gcatcgtttt
    14041 actgctcagc gagctgggag agttatgcgt ctgcgatctt tgtactgccc tcgatcagtc
    14101 gcagcccaag atctcccgcc acctggcatt gcttcgtgaa agcgggctat tgctggaccg
    14161 caagcaaggt aagtgggttc attaccgctt atcaccgcat attccagcat gggcggcgaa
    14221 aattattgat gaggcctggc gatgtgaaca ggaaaaggtt caggcgattg tccgcaacct
    14281 ggctcgacaa aactgttccg gggacagtaa gaacatttgc agttaaaaat ttagctaaac
    14341 acatatgaat tttcagatgt gttttatccg ggaggcatta tgttactggc aggcgctatc
    14401 tttgtcctga ccatcgtatt ggttatctgg cagccgaaag gtttaggcat cggctggagt
    14461 gcaacgctcg gcgcagtact ggcgttagtt acgggcgtgg ttcatccggg tgatattccg
    14521 gtggtgtgga atatcgtctg gaacgcgacg gctgcgttta tcgccgtcat tatcatcagc
    14581 ctgctgctgg atgagtccgg cttttttgaa tgggcggcgc tgcacgtctc acgctggggt
    14641 aatggtcgtg gtcgcttgct gtttacctgg attgtcctgc tcggtgctgc cgttgccgcc
    14701 ctgtttgcca atgatggcgc ggcgcttatt ttgacgccga ttgtcatcgc catgctgctg
    14761 gctttaggat tcagtaaagg cacgacgctg gcgttcgtga tggcggccgg attcattgcc
    14821 gataccgcca gcctgccgct tattgtctcc aacctggtga atatcgtttc cgctgatttc
    14881 tttggcctcg gctttcgcga atacgcctcg gtgatggtgc cggtggatat cgccgcgatt
    14941 gttgccacgc tggtgatgct acatctctat tttcgcaaag atattccgca gaactacgat
    15001 atggcgctgc tgaaatctcc cgcagaagcg atcaaagatc ctgctacgtt caaaactggc
    15061 tgggttgttt tactgcttct gctggtggga tttttcgtcc tggaaccgct cggcattccg
    15121 gtgagcgcca ttgcagctgt gggcgcgctg atattatttg tcgtcgctaa acgcggtcat
    15181 gcgattaata cgggtaaagt cctgcgcggt gccccctggc agattgtcat cttctcgctc
    15241 ggcatgtatc tggtggttta tggcctgcgc aatgccggat taacggaata tctttctggc
    15301 gtactcaacg tgctggcgga taacggcctg tgggccgcga cgctcggcac cggattcctc
    15361 accgccttcc tctcttctat tatgaacaat atgccgacgg tactggttgg cgcgttgtcc
    15421 attgatggca gcacggcatc tggcgttatc aaagaagcga tggtttatgc caatgtgatt
    15481 ggctgcgatt tgggaccgaa aattacccca attggtagcc tggctacgct actctggctg
    15541 cacgtacttt cgcagaagaa tatgactatc agctggggat attacttccg tacagggatt
    15601 atcatgaccc tgcctgtgct gtttgtgacg ctggctgcgc tggcgctacg tctctctttc
    15661 actttgtaat gagatactga tatgagcaac attaccattt atcacaaccc ggcctgcggc
    15721 acgtcgcgta atacgctgga gatgatccgc aacagcggca cagaaccgac cattatccat
    15781 tatctggaaa ctccgccaac gcgcgatgaa ctggtcaaac tcattgccga tatggggatt
    15841 tccgtacgcg cgctgctgcg taaaaacgtc gaaccgtatg aggagctggg ccttgcggaa
    15901 gataaattta ctgacgatcg gttaatcgac tttatgcttc agcacccgat tctgattaat
    15961 cgcccgattg tggtgacgcc gctgggaact cgcctgtgcc gcccttcaga agtggtgctg
    16021 gaaattctgc cagatgcgca aaaaggcgca ttctccaagg aagatggcga gaaagtggtt
    16081 gatgaagcgg gtaagcgcct gaaataaagc ggcgatatcc actgccgata gttggtaaag
    16141 tgcgctttgt ttatgtcgga tgcgacgcta acgcgtctta tccgacctac aaaaccgtgc
    16201 aaattcaata tatggcatga aatatgtaga gcgtagcgca tcaggcaatt ttgctattta
    16261 cgcagcactc ctcgcgtaag aattcaatta tcgcttccag cattgcgtat tgcgaaacac
    16321 acatcagcgt ccgactctca cggttttgtt tgaccagtcc taccgatacc agtgctgata
    16381 tatggtggct gagtgtggag ccaggtatcc caagctgctt ttgcaactca ccgaccggca
    16441 acccttgttc cccggctttg acaagatgtt taaatatgaa caaacgggtc gggtgaccta
    16501 gctctttcag cgctttagcg acttcttcca actgcatatg acacccctct gtctggttga
    16561 tatttcgata ataccagaaa tatcttgatc tgaatttcat ttcgattatt ctggaaatat
    16621 agatttatcc aatgaggtta cgtatgagta gttggcttgc gatgctgcaa gatgccgcag
    16681 agatgtttgt gtttctcgcc gtcgagcttt cgttgctgtt tatagtgatt agtgccggtg
    16741 tcagcctgat aagacaaaag gtgccagacc ataaaatcca gcagatgatg ggggccagaa
    16801 aagggagagg ctatctcctg gctgctctgt tgggagccgt taccccgttc tgtagttgct
    16861 cgacaatacc catgttacgt ggattgttgt cagcgaaagc cgggt
//
LOCUS       DAZOZY010000078        16642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZOZY010000078 DAZOZY010000000
VERSION     DAZOZY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.078"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            45..1184
                     /gene="arnB"
                     /locus_tag="ACHURQ_002233"
     CDS             45..1184
                     /gene="arnB"
                     /locus_tag="ACHURQ_002233"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR0823997.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1188..2156
                     /gene="arnC"
                     /locus_tag="ACHURQ_002234"
     CDS             1188..2156
                     /gene="arnC"
                     /locus_tag="ACHURQ_002234"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR0823998.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2156..4138
                     /gene="arnA"
                     /locus_tag="ACHURQ_002235"
     CDS             2156..4138
                     /gene="arnA"
                     /locus_tag="ACHURQ_002235"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR0823999.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4135..5025
                     /gene="arnD"
                     /locus_tag="ACHURQ_002236"
     CDS             4135..5025
                     /gene="arnD"
                     /locus_tag="ACHURQ_002236"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR0824000.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5025..6677
                     /gene="arnT"
                     /locus_tag="ACHURQ_002237"
     CDS             5025..6677
                     /gene="arnT"
                     /locus_tag="ACHURQ_002237"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR0824001.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6674..7009
                     /gene="arnE"
                     /locus_tag="ACHURQ_002238"
     CDS             6674..7009
                     /gene="arnE"
                     /locus_tag="ACHURQ_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR0824002.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7009..7395
                     /gene="arnF"
                     /locus_tag="ACHURQ_002239"
     CDS             7009..7395
                     /gene="arnF"
                     /locus_tag="ACHURQ_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR0824003.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7389..7655)
                     /gene="pmrD"
                     /locus_tag="ACHURQ_002240"
     CDS             complement(7389..7655)
                     /gene="pmrD"
                     /locus_tag="ACHURQ_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR0824004.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7765..9120)
                     /gene="menE"
                     /locus_tag="ACHURQ_002241"
     CDS             complement(7765..9120)
                     /gene="menE"
                     /locus_tag="ACHURQ_002241"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR0824005.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(9117..10079)
                     /gene="menC"
                     /locus_tag="ACHURQ_002242"
     CDS             complement(9117..10079)
                     /gene="menC"
                     /locus_tag="ACHURQ_002242"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR0824006.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(10079..10936)
                     /gene="menB"
                     /locus_tag="ACHURQ_002243"
     CDS             complement(10079..10936)
                     /gene="menB"
                     /locus_tag="ACHURQ_002243"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR0824007.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10951..11709)
                     /gene="menH"
                     /locus_tag="ACHURQ_002244"
     CDS             complement(10951..11709)
                     /gene="menH"
                     /locus_tag="ACHURQ_002244"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR0824008.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11706..13376)
                     /gene="menD"
                     /locus_tag="ACHURQ_002245"
     CDS             complement(11706..13376)
                     /gene="menD"
                     /locus_tag="ACHURQ_002245"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR0824009.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13465..14760)
                     /gene="menF"
                     /locus_tag="ACHURQ_002246"
     CDS             complement(13465..14760)
                     /gene="menF"
                     /locus_tag="ACHURQ_002246"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR0824010.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14839..15144)
                     /gene="elaB"
                     /locus_tag="ACHURQ_002247"
     CDS             complement(14839..15144)
                     /gene="elaB"
                     /locus_tag="ACHURQ_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR0824011.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15199..15660)
                     /locus_tag="ACHURQ_002248"
     CDS             complement(15199..15660)
                     /locus_tag="ACHURQ_002248"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0824012.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15725..16642
                     /gene="rbn"
                     /locus_tag="ACHURQ_002249"
     CDS             15725..16642
                     /gene="rbn"
                     /locus_tag="ACHURQ_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR0824013.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3834 a         4533 c         4283 g         3992 t
ORIGIN      
        1 agcaaatata agtggacagg tattcaatgg cggaaggaaa agcaatgtca gaatttttgc
       61 ctttttcgcg accagcaatg ggcgtggagg aactcgctgc agttaaagag gttctcgaat
      121 ccggttggat cacaaccggt ccgaagaatc aggcgcttga gcaagccttt tgccagttga
      181 cgggaaatca gcatgccatc gcggtcagtt cagccaccgc cggaatgcat atcacgctaa
      241 tggcgttgga aattggcaag ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa
      301 ccctcaatat gatttccttg ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata
      361 cgctgatggt cacgcctgaa gcgatcgagt cagccattac gccacgcact aaagccatca
      421 ttccggtgca ttatgccggt gcgccagcag atattgacgc cattcgcgcc attggcgaac
      481 gttacggcat cgcagttatc gaagatgctg cccatgccgt cggtacgtat tacaaagggc
      541 gacatattgg cgcaaaaggt accgctattt tttcatttca tgccattaaa aatattacct
      601 gtgcggaagg tggcctgatt gtaactgata atgaaaacct tgcccgccag ctacggatgc
      661 tgaaatttca cggtctgggt gtcgatgcct atgacagaca aacctggggc cgtgcaccgc
      721 aggctgaagt cttaacaccg ggctataagt acaatctgac cgatattaac gccgcgattg
      781 ccctgacaca gttagcaaaa ttagagcacc tcaacacccg tcggcgcgaa attgcccagc
      841 aatatcagca agcactggca gctctcccct ttcagccatt aagccttccc gcctggccgc
      901 acgttcacgc ctggcatctg tttattattc gtgtcgatga acaacgttgt ggtatcagtc
      961 gcgatgcgtt gatggaagcg ttaaaagaaa gaggcattgg taccgggtta catttccgcg
     1021 ccgctcacac acaaaaatat tatcgcgagc gttttcccac gctgtcgtta ccgaataccg
     1081 aatggaatag cgaacgcatc tgttctttgc cgctgttccc ggatatgact accgccgatg
     1141 ccgaccgtgt catcacagcc cttcagcaac tcgcaggaca ataagccatg tttgaaatcc
     1201 accctgttaa gaaagtctcg gtggttattc ccgtttataa cgagcaggaa agcttaccgg
     1261 aattaatcag gcgcaccacc acagcctgtg aatcgttggg gaaagagtat gagatcctgc
     1321 tgattgatga cggcagtagc gataattccg cgcatatgct ggtcgaagcc tcacaagcgg
     1381 agaacagcca tattgtgtct attttgctta accgcaatta cgggcaacat tcagcgatta
     1441 tggcgggatt cagtcacgtt actggcgact taattattac ccttgatgcc gatctccaga
     1501 atccgccaga agaaatcccc cgcctggtgg caaaagccga tgaaggttac gacgtggtag
     1561 ggactgtacg ccagaaccgc caggacagct ggtttcgtaa aacagcttcg aagatgatta
     1621 acaggcttat tcagcgcacc accggcaaag cgatgggtga ctacggttgt atgctgcgcg
     1681 cctatcgccg tcatattgtc gatgcgatgt tgcactgcca tgaacgtagc acctttatcc
     1741 cgattctggc gaatatcttc gcccgccgtg ccattgaaat tccagtacat catgccgagc
     1801 gtgagtttgg tgaatccaaa tacagtttta tgcgcctgat taatttgatg tacgacctgg
     1861 tgacctgcct taccacaacg ccgctacgta tgctgagtct gctcggcagc attattgcga
     1921 ttggaggttt tagcattgcg gtgctgctgg tgattttacg cctgaccttc ggaccacaat
     1981 gggcggcaga aggcgtcttt atgctatttg ccgtgctgtt tacttttatt ggcgctcagt
     2041 ttatcggcat gggattgctc ggtgaatata tcggcaggat ctacaccgat gtccgcgccc
     2101 gcccccgcta ttttgttcag caagttatcc gtccatccag caaggaaaat gaataatgaa
     2161 aaccgtcgtt tttgcctacc acgatatggg atgcctcggt attgaagctc tgctggctgc
     2221 cggttacgaa attagcgcca tttttaccca taccgataat cccgctgaaa aagcctttta
     2281 tggttcggtg gctcgtctgg cggcggaaag aggcattcca gtttatgcgc cggataacgt
     2341 taatcatccg ctgtgggtgg aacgcattgc ccaactctca ccagaggtga ttttctcttt
     2401 ttattatcgc catcttattt gcgacgagat tttgcagctc gctcccgcag gtgcatttaa
     2461 tctgcacggt tcgctgttac caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt
     2521 gaacggtgaa acggaaacgg gcgttacatt gcaccgaatg gtaaaacgtg ccgatgctgg
     2581 ggccattgtg gcgcaactgc gcgttgccat tgcgccagac gatatcgcta ttacgctgca
     2641 tcataaattg tgccatgccg cgcgccagct actggaacag acattacccg ccattaaaca
     2701 cggtaatatt ctggaaattg cccagcgcga aaacgaagcc acctgttttg gtcacagaac
     2761 gccagatgac agtttccttg aatggcataa accggcatcc gtactgcaca acatggtacg
     2821 tgccgttgcc gatccgtggc cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt
     2881 ctggtcgtcg cgtgttcatc ctcatgccag caaagcacag ccggggagcg tgatttctgt
     2941 tgcgccactg ctgattgcct gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg
     3001 cgacggcatt actatgcagg gctcgcaatt agcgcagatg ctgggcctgg tgcaaggttc
     3061 acgcttgaat agccagcctg cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg
     3121 ggtgaatggc tttattggca accatctgac agaacgcctg ctgcgcgaag atcattatga
     3181 agtttacggt ctggatattg gcagcgatgc gataagccgt tttctgaatc atccgcattt
     3241 tcactttgtc gaaggcgata tcagtattca ttccgaatgg attgagtatc acgtcaaaaa
     3301 atgtgatgtc gtcttgccgc tggtggcgat agccacgccg attgaatata cccgcaaccc
     3361 gctgcgcgta tttgaactcg attttgaaga gaatctgcgc attatccgct actgcgttaa
     3421 gtaccgtaag cgaatcatct tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa
     3481 atacttcgat gaggaccatt ctaatttaat cgtcggcccg gtgaataaac cacgctggat
     3541 ttattcggtg tcaaaacaat tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg
     3601 tttacagttc accctcttcc gcccgtttaa ctggatggga ccacgactgg ataaccttaa
     3661 tgcagcgcga attggcagct cccgcgctat tacgcaactc attctcaatc tggtagaagg
     3721 ttcaccaatt aagctaattg atggcggaaa acaaaaacgc tgctttactg atatccggga
     3781 cggtatcgag gcgttgtacc gcattatcga aaatgcggga aatcgctgcg acggcgaaat
     3841 tatcaacatt ggcaatcctg agaacgaagc gagcattgag gaactgggcg agatgctgct
     3901 ggcgagcttc gaaaaacatc cgctgcgcca tcacttccca ccgtttgcgg gctttcgtgt
     3961 tgtcgaaagt agcagctact acggcaaagg atatcaggac gtagagcatc gtaaaccgag
     4021 catccgcaat gcccgccgct gcctggactg ggaaccgaaa attgatatgc aggaaaccat
     4081 cgacgaaacg ctggatttct tcctgcgcac cgttgatctt acggataaac catcatgacc
     4141 aaagtcggct tacgcattga tgtcgatacc tttcgtggca cccgtgaagg cgtgccacgt
     4201 ctgctggaaa tcttgagtaa gcataatatt caggccagca tttttttcag cgtcggcccg
     4261 gacaatatgg gccgccatct ctggcgactg gtgaagccac agtttttgtg gaagatgctg
     4321 cgctcaaacg cggcatcgct ttatggctgg gatattttac tggcaggtac ggcctggcca
     4381 ggtaaagaga ttggtcatgc caatgccgat atcattcgtg aagcggctaa acatcacgaa
     4441 gtcggcctgc acgcctggga tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg
     4501 caaacaatga tcgacgatat tgcacgcggt cttcgcactc tggaagagat tatcggtcaa
     4561 ccggtaacct gttctgccgc tgcgggctgg cgtgccgacc agaaggtgat cgaagcaaaa
     4621 gaagcgttcc atttgcgcta caacagcgat tgtcgtgggg ccatgccgtt ccgtccactg
     4681 ctcgaatcag gaaaccctgg cactgcgcaa attccggtga ccttacccac ctgggatgaa
     4741 gtgattggtc gggatgtgaa agcagaagat tttaacggtt ggttactcaa ccgcatcctg
     4801 cgagataaag gcacgccggt ttataccatt catgcagaag ttgaaggctg cgcttatcag
     4861 cataattttg tggatctcct caaacgcgca gctcaggaag gcgtgacatt ttgcccttta
     4921 agcgaactgt tatcagagac gttgccgctc ggacaagttg ttcgcggaaa tattgccgga
     4981 cgtgaaggct ggctgggttg ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt
     5041 accttatcgg cctcttcgcg tttattgcct gctattacct gttaccgatc agcacgcgtc
     5101 tgctctggca acccgatgaa acgcgttatg cggaaatcag tcgagaaatg ctggcatccg
     5161 gcgactggat tgtgccccat ctgttagggc tacgttattt cgaaaaaccc attgccggat
     5221 actggattaa cagcattggg caatggctat ttggcgcgaa taactttggt gtgcgggcag
     5281 gcgttatctt tgcgaccctg ttaactgccg cgctggtgac ctggtttact ctgcgcttat
     5341 ggcgcgataa acgtctggct ctactcgcca cagtaattta tctctcattg tttattgtct
     5401 atgccatcgg cacttatgcc gtgctcgatc cgtttattgc attctggctg gtggcgggaa
     5461 tgtgcagctt ctggctggca atgcaggcac agacgtggaa aggcaaaagc gcaggatttt
     5521 tactgctggg aatcacctgc ggcatggggg tgatgaccaa aggttttctc gcccttgccg
     5581 tgccggtatt aagcgtgctg ccatgggtag cgacgcaaaa acgctggaaa gatctcttta
     5641 tttacggctg gctggcggtt atcagttgcg tactgacggt tctcccctgg ggactggcga
     5701 tagcgcagcg ggagcctgac ttctggcact attttttctg ggttgagcat attcaacgct
     5761 ttgcactgga tgatgcccaa catagagctc cgttctggta ctacgtgccg gtcatcattg
     5821 ccggtagcct gccgtggctg ggattactcc ccggtgcact gtacacaggc tggaaaaacc
     5881 gcaagcattc cgcaaccgtc tatttgttga gctggacgat aatgccgctg ctgtttttct
     5941 ccgtcgctaa aggtaaattg cccacctata ttctttcctg ctttgcatct ctggcaatgc
     6001 tgatggcgca ttacgctttg ctggcagcaa aaaataatcc tctggcgctg cggattaatg
     6061 gctggattaa catcgctttt ggcttcactg gcattattgc cacatttgtg gtctccccgt
     6121 ggggaccaat gaacacgccg gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct
     6181 ggtcgatttt ttcgctatgg gcatttttcg gctggtacac cttaacaaac gtcgaaaaga
     6241 cctggtcttt tgccgcgctt tgcccgctgg ggctggcgtt gctggtagga ttttcaattc
     6301 ctgacagagt catggaagga aaacatccgc aattttttgt cgagatgaca caagaatcac
     6361 ttcagccaag ccgctatatt cttactgata gcgtcggtgt tgccgcaggt ctggcatgga
     6421 gcctgcaacg cgatgacatc atcatgtatc gccagacagg tgagttgaaa tacggcctta
     6481 attatccgga tgcgaaaggg agatttgtca gcggtgatga gttcgcaaac tggcttaatc
     6541 aacatcgtca ggaggggatt attacactcg tgctttcggt tgaccgcgat gaagatatca
     6601 acagtctcgc cattccgccc gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc
     6661 agtatcgtcc caaatgatct ggctaacatt agtctttgcc agcttgctta gcgttgccgg
     6721 gcagttgtgt cagaaacagg caacctgctt tgtggcgata aacaaacggc gcaaacatat
     6781 cgtgctgtgg ctgggactgg cgctggcttg tcttggtctt gccatggtgc tctggctgct
     6841 ggtcttgcag aacgtaccgg taggcattgc ctacccgatg ttaagtctga attttgtctg
     6901 ggtgacgctg gctgcagtaa aactgtggca cgaaccggta tcgccgcgtc actggtgtgg
     6961 ggtggcgttc attattggcg gcattgtgat cctcgggagt acggtgtaat gggcctgatg
     7021 tggggattat tcagcgtcat tattgcctca gttgcgcaat taagcctggg ttttgcggcg
     7081 agtcatctgc cgccgatgac gcacttatgg gattttatcg cggctctgtt agcttttggc
     7141 ctggatgccc ggatactgtt actcgggctg ctgggttatc tgctttccgt tttttgttgg
     7201 tataaaacgc tacataaact tgcgctaagc aaagcctatg ccttgctcag tatgagttat
     7261 gtgctggtat ggattgcttc aatggtactt cccggctggg aagggacttt ttcgctgaaa
     7321 gcactactgg gagtagcctg tattatgagc gggttgatgc tgattttcct gcccacgaca
     7381 aaacaacgtt actgagtttt ccctgccact ttacactgcc gttcccactc atcggggcta
     7441 taactgcttg ccgagaggac tttcaccgta tgcagctttt cacgattaat acaataaagc
     7501 gcattctgca aaggcgagag taaatccccc actttcacgg cgaagtcact cttcacttcg
     7561 gcgatcattt ttatcgcgcc gccagcatcg cagagcataa gtacatgtct gttatcttgt
     7621 ttattgcagc acgatttttt gaccagccat tccattgcat tatcctgttt gctaagagtt
     7681 ttcacatcaa tttacagatt aacagataaa cctattatca acggcaatgg aatggcaaaa
     7741 ttgcgaagtg aaactaatga tttcttaatc ctggcgttgc acccactctt ttaacgcctg
     7801 acgtgaaatt ttaataccgc cgtttttcag ctccggcggt aaagttagcc agcgcaccgg
     7861 ttgttgaaaa cgagccagct tatctttcac ccattcgcca agatcaacgg tttgctggtc
     7921 atactccacc accgctaccg gtcgatgacc aaactccttg tcggcaacgg ggacgataaa
     7981 cacctgcaaa accgcaggat gtgcagcaat tacgcgctcg acttcttccg gctgaatacc
     8041 ctctccgcca ctgaagaaca gattgtctaa acgtccgaca atggtcagct tgccattatg
     8101 catctcaccg cgatcgcgcg tagcgtacca gccttcgtca ttaaccagtg aaaccagttg
     8161 cccgttacgc cagtaacctt ctgccatact ggcagcccgc agccacactt cattattaac
     8221 gattttcact tcccgacccg gcagcggcga accaacgtct gccaggccgt cggcttcttt
     8281 cgcacacacc gtggaggcaa actcggtcag accatagccg caaaagcaac gaatcccctg
     8341 ctcgcgcgcc tgttccgtca actcgaccgg gatagccgcg ccgccaagta acaccgcttt
     8401 cagggaaacg gaactgcggt taaccagcaa acgccagagt tgtgttggca ccagtgaagc
     8461 gtgagtacag cctgccagca tttgctccaa tggctgttta tcacgcaccg tcatccgcgc
     8521 accagcgtat aaccagcgcc acataattcc ctgaccggag acgtgaaaca gcggtaaaga
     8581 gagcaaccag tcatcgtgat cgccaaacgg aatcagcgat aacacacctt gcgcactggc
     8641 aagatgggcc tgataagtat ggacagcggc tttcggcaaa ccggtagaac ccgaggtcaa
     8701 cgtcattgag cacagacgcg tcggctgcca cgtagcggca tgtgcgcctt caacccgctg
     8761 aatgtgcagc gacgttaatg ccggaaacgt gttttcccca tccggcacca gcgcaaattg
     8821 cagcgtcaga ttgggcagca attcttcaag caacggttgc ggcagctgag ggttcacggg
     8881 caacacccgc gccccgcatt gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt
     8941 ccacgcccgc aacatcacgc cgctgccctc aaccacaccc tgcgccgcaa agcccgtagc
     9001 taactcatcg acgcgagcac aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa
     9061 ggcgatggct tctccccgca cttgccgcca gtgacgccac ggccagtcag agaagatcat
     9121 aacaactgct ccagtacatc agcgtccact aacggcagtg ggctccccgg ccagcgacga
     9181 acctgctgcg cctgcatcag atccagcgtg tccagtcccg gaatggtgtc tggcgttaac
     9241 caggcagcaa tccgcgccag ttgcgttaga cctaagctcg actcaatgga agaactgatc
     9301 accgccgtca gccccagcgc atgcgccgcc tgtacctgct cgcgtacttt ttccagactg
     9361 cccgtgagcg tgggtttgat aactaccgcg cgcacgccct cttcagccac aaaggcaaaa
     9421 tccggctcgc gcaggctttc atcccaggca atggcaatgc cggtttcacg ggcaaacgct
     9481 cgcgaatcat cgcgggtttt gcacggctct tcgagaaacg cgatgcggtg gcgataatcc
     9541 gggttaacat atttggcaaa ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca
     9601 agacgcaaat gcagatccgg aattgcctcc agcaacagat taaccaccat gccgtcgcgc
     9661 acggcttcgt acaatcccac tttgaccttc gccactttct cgcctggcat atcagcaagt
     9721 ttgaggatca gatcgtcagg atcgccatta cacagtggtg ccgcacggta attggccgcc
     9781 tgcgggagcg tatctgccag ctccgccaat gcacagctta cgccaaaggc aacggaaggc
     9841 atctgcggta gctcgccatc gcctgccagc cagttattta cccaagcaag cagcacaatt
     9901 tgcgcctctt cccaggtttc ctgactgaag cccggcagtg gggagatctc cccccaccct
     9961 tcgcgctcgc cttcacgcag gcaaacatac agcccgtcgc gggtttttaa ccgcctgtcg
    10021 cgcagaacca tccccgcgtc catggggatc tgccagcggt atacctgcgc gctacgcatt
    10081 acggattccg tttaaatttg ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac
    10141 cttcctgacc ttcttccgtc atgtagaaca gcatggtggc gttgcccgcc agctcctgca
    10201 gacccgcctg accgtcacag tcggcgttca gcgcagcttt caggcagcgc agcgccatcg
    10261 ggctgttttg cagcatttcg cggcaccaac ggacggtttc tttttccaga tccgccagcg
    10321 gtaccacggt gttgacaagg cccatatcca gcgcctgttt tgcatcgtac tgacggcaca
    10381 ggaaccagat ttcacgcgct tttttctgtc cgacgatgcg agccatgtag gaagcgcccc
    10441 agccgccgtc gaaggaaccg actttcgggc cagtctgacc gaagatggca ttatctgccg
    10501 cgatagtcag gtcgcacatc atgtgcagaa cgtgaccacc gccgatggag tagccagcca
    10561 ccatcgcgac aaccggtttc ggacaggtac ggatctgacg ctggaagtcc agcacgttca
    10621 ggtgatgtac gccggaatca tctttatagc cgccgtaatc accacgcact ttctggtcac
    10681 caccggagca gaacgcttta tcgcctgcgc cagtcagaat gatcacgccg acgttgtcgt
    10741 cataacgcgc atctgccagt gcctggatca tctcttttac cgtcagagga cggaaggcat
    10801 tgcgcacctg cggacgatta atggtgattt ttgcgatacc gtcggtggat ttttcataac
    10861 gaatgtcctc aaaaccttcg gagcagtcgt gccattcaac cggtgcgtaa agcattgctt
    10921 catcaggata aatcatattg tgtcctttag tcagaaacgc aagatctgcg ccagacttgc
    10981 gataacgcca gcgggatttt cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca
    11041 gtcggcagcc agttccgccg ccaaggcgcg gaatttgctg tcacgttcac cacataaata
    11101 ataaaacgca aatgtgcggg cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga
    11161 agtcgcctcc agcatggcgg caagggttgc gccattattg ttactgcgca gcgccaccag
    11221 ctcccggcgt tgatcgtcat tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa
    11281 taccgccgtt aacggttctg tgcgaaaacg ctgcgcccat tggcgatcgg aacgctgacg
    11341 ttccgcgcgt tgttcagcat tttgcaaccc aggatgcccg ccttcgacaa caaccccaca
    11401 aagccccggc atcccctggc aagccgccat catcgccacc cgtcccccaa gcgagtaccc
    11461 caccaaccag aagttaagga tgttgtaact aaccagggtt ttacgcagta agtcggtgac
    11521 atcatcaaat ccatcgacgc taatcgccgc cgaaccaccg tgacctggga gataaacata
    11581 caaccgtgag tagtcggcaa acgcctcgcc cacttcttgc cattcgtggc aatcgccgga
    11641 aaaaccgtgc aaaaacacca gccagggtaa acctggtttg ccgtgttttg cctgcgcgtg
    11701 caggatcata aatggcttac ctgcgccaga agttgctgga gcgtttgcgc accatcggtg
    11761 tcgttaacca ccatttcaat taccgtggtg gttggcgtgc gccaggcatc ggcaagtgcc
    11821 gtttcaagtt cctgccagtt ttgcggacga tgatatttca gctcgaacat cgcggcggcg
    11881 tgctcaaaat ggacgttttg cggcatcaga tagaaacgct cgcgctcgct tttcggcgtt
    11941 ggcagcagcg agaaaatttg cccgccgttg ttgttcacca caattaatat cagcggcgcg
    12001 gaaacctgac gcaataacgc cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca
    12061 atcgccagcg tcggtttgcc gcttgcccgc tgaacgccgg cggcagtcga gagcagcccg
    12121 tcgataccgc tggccccacg gttgctgtac accgggtaac ctgccggaag ttgcgaaagc
    12181 gcatcaatca gacgtaccac caggctgtta ccgacaaaca attgcccctg ttcaggcaga
    12241 tagtcgctga tgcgatgcgc cagttgcgct tcgccaaacg catcgcgacg ggcaataacc
    12301 gcctgcattg cctgttccgc caggcgcggg atttcaacgc accagggctg gcgtttttct
    12361 gccggatgca gctccagcca gtcggcaata ttggcaatta agcggcgtcc acgatggtgc
    12421 gccggatcaa gccgcccttc gatgtcatcc acaatccagt actcttcggg ttcacagctt
    12481 gcctgccatt gcaggagccg tttgcccgtc aggctgcttc ccagttgcac cacaatttgc
    12541 gcctgttgca gttcgctggt cgctttggca ttgcctaacc agagatcggc acacggcagc
    12601 ggctgtccgg tttgcgacag cacgtcgcca atcagcggcc agccaagagt ttgcgcccac
    12661 agtgcaactt ttttgccctc ttccgcactc atgcgcccgg caaccaccac gccgcgtttt
    12721 tgtcgccaga agaaccagtc gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc
    12781 cacggtttgt cgtcctgcca ccagtcgccc agccgctgtt gccagctaag cccggtgtcg
    12841 tccatttcgc catacagcgg ttcggcaaac gggcagttga tatggactcc accagaatga
    12901 agcgtaccga gagcatggtc gatggtagaa accagccaac gtgcggggat atcctgggtc
    12961 gggcgcggca atgaaatact gtgcgtgggg tgagaggcga acattcccgg ctgacgaatc
    13021 gcctgattcg cgccgcagtc aatcagctcc ggcgggcgat cggcggttaa caggatcagt
    13081 ttttctccgg ttaacccagc ttcaatcagt gccggataga gatttgctac cgccgtaccg
    13141 gaggtcacaa tcaccgccac cggctgcttg ctgactttcg ccagccccag cgccagatgc
    13201 cccaacccac gctcatcgaa atgggtgtgg tgaatgaatg cggaattctc cgccgccgct
    13261 aacgttaacg gtgtagaacg cgagccaggg gcgatacaga tgtgtctgac gccgtgacgc
    13321 gttaatgctt ccagaatgac cgccgcccag cgtcggttaa atgcgcttac tgacatgagt
    13381 ttgtccggta tcaatattgc ggctaagtat aaggagctac aaaaatagat tattgatatg
    13441 aatcggtaat gatgcgactc attactattc catctgtaat aaagtacgca gccctgccgc
    13501 tttgttgtcg atttcctgcc actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc
    13561 agcatataat cgcacgacat tgccgctaat ttttgctgag cgcagggaaa cgcagaattc
    13621 gctttgctgt aatgagagat agcccgcaga accggcgtac cattctcggg tgaacggttc
    13681 gtgacgggcg ataaactgtc gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg
    13741 ctgcaactga tgtaaacaga tcacattatc cgctttgttg agtgaagtcc agatacagcg
    13801 gcgaagatgc tgcactttac gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg
    13861 ggtatcggcc tgtaaccgtt ggcagatatc ttccaccacc agcatattct cgcgctggtt
    13921 tttatcatcc gccatcagcc actcacctaa ctgctgcgcc tgcttatcat caggatgatt
    13981 tgctaccgtt cccgccagcg cttcagtacg cagcgctttg tcacgccgcc gccataaccg
    14041 ttccggtgaa gagccaagaa aagcattttc gccatcaaag gccatgtaaa aatggtagca
    14101 attcagattc agtcgacgac tggcagccat catcgccgcc gcgttgaccg gacttgcgaa
    14161 atgcaggtca gttgcccgag cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt
    14221 cgttgccagt tcgattaatt gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt
    14281 ggtggttaaa tgtaacccag gcaagggctt gatactcacc agtgtggcga taaattctct
    14341 tgccttaatc gcatcgtgct gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc
    14401 tttaccgcca cagcgtcgcc attccaggcg tggtaaaagt aaattgccct gcgacgggtc
    14461 aaacgcattc agcccccaaa tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg
    14521 ttgtgcctgg tccaacgacg taaaacgggt aatcgcgccc aggacggcag cttcttcatc
    14581 accattacgt tgttgccagt agaattgcgg gtatgtttgc tgactggcca gccagctcaa
    14641 ggcatcaaaa gcgtctttga gagggaaagg aatatcgata acccgaatgc cgggtgtcgc
    14701 cggaatctct tgcgacaaat ggcgcagtag attttccagc gccgtagtaa gtgattgcac
    14761 gcggacctct ccctgttaaa agcctcacat tatacggggt actacaaaaa aaatgcagta
    14821 ccccggtgta gggaggtttt aacggcgtgc cagcaacagt cctagtacca gcccaacggc
    14881 cgcgcccaca ccaattcctt gccagggttt ttcgtggacg tagtcatcag cacgataaac
    14941 tgcctgcttc gcccgatagt aataactgtc tgaagcctgg ctaacccgtt ttttcacatc
    15001 atccagcgct ttttctgcac gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc
    15061 agaggagcgg agcacctctt ccagtgtttc actaagcagc gtcaggtcgt catcgatacg
    15121 tgtatcacca aactgattag acattctcgt tctccatttg cgtaaaacct gtcactaact
    15181 atagacaagg gttgttgatt acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat
    15241 accatcttct tcatagacct ccgtcacagg gataaaaccg aaactctggt agaagttttg
    15301 cagatgcgcc tgcgccccga ggtaaacagg cttatcaggc cagtgatgcg tgcaggtgtc
    15361 gagggtttta ctcatcagtt gctgccccac tttttcgcca cgcaacgctt cactgacaat
    15421 cacccgacca ataacgaccg gctcaagatc atcatcactt ttcagaatcc tcgcatatgc
    15481 caccagttca tcatttttcc aaccgagaat atggcgatta tcccccgtca aatcatcacc
    15541 atcgatatcc tgataagggc aattctgctc gacaacaaac accgcgcaac gcagttgtaa
    15601 taaggcataa agctgggaaa ccgataattc agagtgatgc agatcttgcc attcaatcat
    15661 aatgctctcc ttttagatgt atgccttata ctagccggaa tattttttga aacgtgatga
    15721 actcatggaa ttaatttttt taggtacttc agccggtgtg ccaacccgca cgcgcaatgt
    15781 cacggcaata ttgcttaacc tgcaacatcc gacccagagc ggactttggt tgtttgactg
    15841 cggtgaaggc acccagcatc agctactgca taccgccttt aaccctggga aactggacaa
    15901 gatttttatc agtcaccttc atggcgatca tctctttggt ttacccggct tgctgtgcag
    15961 tcgttctatg tcaggcatta tccaaccctt aacgatttat ggtccgcaag gtatccgtga
    16021 atttgtggaa accgcgctgc ggattagcgg ctcatggacc gattatccgc tggaaattgt
    16081 cgaaattggc gctggcgaaa ttctcgatga tggcctgcgc aaagtgaccg cttatccgat
    16141 ggaacaccca ctggaatgtt atggctatcg tattgaagaa catgataaac cgggtgcatt
    16201 aaatgcccag gcattaaaag ctgctggcgt gccgcctggc ccactgtttc aggaattaaa
    16261 agcgggcaaa acaatcacgc tggaagatgg aaggcagatt aacggcgcag attacttagc
    16321 tgctccagtg ccaggtaaag cgctcgctat tttcggcgat accggcccct gcgatgccgc
    16381 acttgacctg gctaaaggtg tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat
    16441 ggaagccaaa gccaatagtc gcggccatag ctctacacgc caggctgcga cactagcccg
    16501 tgaggctgga gtcggcaagc taatcattac ccacgtcagc tcgcgctatg atgacaaagg
    16561 ttgtcaacac ctgttacgtg aatgcaggtc aattttcccg gcgactgaac tggcgaatga
    16621 tttcaccgtg tttaacgttt aa
//
LOCUS       DAZOZY010000079        16464 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZOZY010000079 DAZOZY010000000
VERSION     DAZOZY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16464
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.079"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHURQ_002250"
     CDS             complement(<1..170)
                     /locus_tag="ACHURQ_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR0824014.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHURQ_002251"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHURQ_002251"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR0824015.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHURQ_002252"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHURQ_002252"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR0824016.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHURQ_002253"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHURQ_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR0824017.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHURQ_002254"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHURQ_002254"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR0824018.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHURQ_002255"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHURQ_002255"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR0824019.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHURQ_002256"
     CDS             4288..5151
                     /locus_tag="ACHURQ_002256"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR0824020.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="ACHURQ_002257"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="ACHURQ_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR0824021.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="ACHURQ_002258"
     CDS             6489..7106
                     /locus_tag="ACHURQ_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR0824022.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHURQ_002259"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHURQ_002259"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR0824023.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLAHKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="ACHURQ_002260"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="ACHURQ_002260"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR0824024.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHURQ_002261"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHURQ_002261"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR0824025.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="ACHURQ_002262"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="ACHURQ_002262"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR0824026.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="ACHURQ_002263"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="ACHURQ_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR0824027.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="ACHURQ_002264"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="ACHURQ_002264"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR0824028.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHURQ_002265"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHURQ_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR0824029.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
BASE COUNT         4077 a         4479 c         4176 g         3732 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggct
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttatgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacttgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaactt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggca tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca ttta
//
LOCUS       DAZOZY010000080        16402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZOZY010000080 DAZOZY010000000
VERSION     DAZOZY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.080"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHURQ_002266"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHURQ_002266"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR0824030.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHURQ_002267"
     CDS             complement(2380..2820)
                     /locus_tag="ACHURQ_002267"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0824031.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHURQ_002268"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHURQ_002268"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR0824032.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHURQ_002269"
     CDS             3538..4236
                     /locus_tag="ACHURQ_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR0824033.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHURQ_002270"
     CDS             4465..5673
                     /locus_tag="ACHURQ_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0824034.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHURQ_002271"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHURQ_002271"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR0824035.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHURQ_002272"
     tRNA            7780..7856
                     /locus_tag="ACHURQ_002272"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHURQ_002273"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHURQ_002273"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHURQ_002274"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHURQ_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR0824036.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHURQ_002275"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHURQ_002275"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR0824037.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHURQ_002276"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHURQ_002276"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR0824038.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHURQ_002277"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHURQ_002277"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR0824039.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14612
                     /gene="dppF"
                     /locus_tag="ACHURQ_002278"
                     /pseudo
     CDS             13606..14612
                     /gene="dppF"
                     /locus_tag="ACHURQ_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
     gene            complement(14642..15913)
                     /locus_tag="ACHURQ_002279"
     CDS             complement(14642..15913)
                     /locus_tag="ACHURQ_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR0824040.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3900 a         4096 c         4409 g         3997 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccccggac gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa
    14461 gcccactgaa tccaccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc
    14521 cctgcaccca gttgcagccg cagctaaaag actacggcgg tcaactggta gcttgttttg
    14581 ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca tgcgctccgg
    14641 tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtac agcagaatca
    14701 gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc aggaaaaacg
    14761 gaatgaggca cgacacaata ccatataacg ggcttcccaa ctgaaagaac accagcaccg
    14821 atacacgaaa cgaaacccaa atcgtcaacg taatgacgat aaaagcgcag atcgccagag
    14881 tcagcacgcg tgagttaatt ttcttggtat caataattcg gcttaacaga ttgagaataa
    14941 tgcctttaat ggcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa
    15001 agatattaag caccgtagag gtgatatgaa tgatatgccc agggatcacc tgcgcggcca
    15061 gcgccagtgc tgagatattt tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga
    15121 aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa ctaattcggt
    15181 gggtacgcag cgcgagccgg gttgccagta ctttatccgc ttcccgttta cgataggcaa
    15241 tattcattgg gttaagtacc tgaataaata ctgcagaaaa gaagcaaaat ggaatggtaa
    15301 gcaagacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg gcgaaattcc
    15361 agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc accaccattg
    15421 gcccggaaat cttaaacagt aatcgttcac caccagacgc aatcgccacc agtacggcga
    15481 aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa ccgaaggttt
    15541 tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc ccgtggataa
    15601 tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg taatgactga
    15661 taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa taaatgtcct
    15721 gcactaccca ggtggcagga taagcaatga tcgctgcggt aataaatacc caaattccct
    15781 tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg cctatgcata
    15841 ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg
    15901 tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc
    15961 agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac
    16021 agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat ttcactacca
    16081 gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt aagctggaag
    16141 gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc
    16201 ggcaagccga aagggttccg gtgaggcgca atgttgcggg ggctttatcc ctggtggcat
    16261 tggttgctgg agagagaaaa cccccgcacg ttgcaggtat gtacctgaca acataacggg
    16321 ggctaatctt gaacctagac cactcaagaa tagccgcgaa acgttgtcat tacaacacag
    16381 gcggctatat gacgctcgca ga
//
LOCUS       DAZOZY010000081        16256 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZOZY010000081 DAZOZY010000000
VERSION     DAZOZY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16256
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.081"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(87..1289)
                     /gene="ubiI"
                     /locus_tag="ACHURQ_002280"
                     /gene_synonym="visC"
     CDS             complement(87..1289)
                     /gene="ubiI"
                     /locus_tag="ACHURQ_002280"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR0824041.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1312..2490)
                     /gene="ubiH"
                     /locus_tag="ACHURQ_002281"
                     /gene_synonym="visB"
     CDS             complement(1312..2490)
                     /gene="ubiH"
                     /locus_tag="ACHURQ_002281"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR0824042.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2487..3812)
                     /gene="pepP"
                     /locus_tag="ACHURQ_002282"
     CDS             complement(2487..3812)
                     /gene="pepP"
                     /locus_tag="ACHURQ_002282"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR0824043.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3838..4416)
                     /gene="ygfB"
                     /locus_tag="ACHURQ_002283"
     CDS             complement(3838..4416)
                     /gene="ygfB"
                     /locus_tag="ACHURQ_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR0824044.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4584..4913
                     /gene="zapA"
                     /locus_tag="ACHURQ_002284"
     CDS             4584..4913
                     /gene="zapA"
                     /locus_tag="ACHURQ_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR0824045.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4955..5138
                     /gene="ssrS"
                     /locus_tag="ACHURQ_002285"
     ncRNA           4955..5138
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHURQ_002285"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5159..5761
                     /locus_tag="ACHURQ_002286"
     CDS             5159..5761
                     /locus_tag="ACHURQ_002286"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR0824046.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5862..5921)
                     /locus_tag="ACHURQ_002287"
     CDS             complement(5862..5921)
                     /locus_tag="ACHURQ_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR0824047.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6253..6312)
                     /gene="ibsC"
                     /locus_tag="ACHURQ_002288"
     CDS             complement(6253..6312)
                     /gene="ibsC"
                     /locus_tag="ACHURQ_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR0824048.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6541..7773)
                     /gene="serA"
                     /locus_tag="ACHURQ_002289"
     CDS             complement(6541..7773)
                     /gene="serA"
                     /locus_tag="ACHURQ_002289"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR0824049.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8029..8688)
                     /gene="rpiA"
                     /locus_tag="ACHURQ_002290"
     CDS             complement(8029..8688)
                     /gene="rpiA"
                     /locus_tag="ACHURQ_002290"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR0824050.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9118..10011
                     /gene="argP"
                     /locus_tag="ACHURQ_002291"
     CDS             9118..10011
                     /gene="argP"
                     /locus_tag="ACHURQ_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR0824051.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10214..12358
                     /gene="scpA"
                     /locus_tag="ACHURQ_002292"
     CDS             10214..12358
                     /gene="scpA"
                     /locus_tag="ACHURQ_002292"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR0824052.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12351..13346
                     /gene="meaB"
                     /locus_tag="ACHURQ_002293"
     CDS             12351..13346
                     /gene="meaB"
                     /locus_tag="ACHURQ_002293"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR0824053.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13357..14142
                     /gene="scpB"
                     /locus_tag="ACHURQ_002294"
     CDS             13357..14142
                     /gene="scpB"
                     /locus_tag="ACHURQ_002294"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR0824054.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14166..15644
                     /gene="scpC"
                     /locus_tag="ACHURQ_002295"
     CDS             14166..15644
                     /gene="scpC"
                     /locus_tag="ACHURQ_002295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR0824055.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15641..>16256)
                     /locus_tag="ACHURQ_002296"
     CDS             complement(15641..>16256)
                     /locus_tag="ACHURQ_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0824056.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3946 a         4382 c         4138 g         3790 t
ORIGIN      
        1 ttatttcaaa tgaggaaaat ctcccggcga aaaaaccggg agatgaaagt gtgatgggta
       61 tcaaataaac agcagaagag aaatttttaa cgcagccatt caggcaaatc gtttaatccc
      121 attgcctggc ggataagttg tggtttaacg ccaggaagcg tgtcggccag tttcaaacca
      181 atatcacgca gcagtttttt cgccggattg gtaccggaaa acagatcgcg gaatccctgc
      241 ataccagcca gcatcagcgc cgcactgtgc ttgcggctac gctcataccg gcgcaggtaa
      301 atgtactgcc cgatgtcttt cccctgacga tgcaaccgtt tcagttcagc aatcagctct
      361 gcagcatcca taaagccaag atttactccc tgccccgcca gcgggtgaat ggtatgcgcg
      421 gcgtcgccca ccagcaccag acggtgcgcg gcaaactggc gcgcataacg ccccgtcagt
      481 gggaacacct gacgcgcgct ctcaaccttg cataagccca ggcgattatc aaaagcgata
      541 tttaacgcgc gattaaattc atcttcactt gcctgctgca tccgctgcgc ttcctctggc
      601 gacagtgacc agacaattga gcaaagatgc ggatcgctaa gcggtaaaaa ggccagaatg
      661 ccttcgccat ggaaaacctg ccgcgccacc gcatcatgcg gttcttccgt gcgaatggtc
      721 gctaccagcg cgtgatgctg ataatcccag aaagtcagcg gaatatctgc tttgttgcgc
      781 aaccaggaat tagcaccgtc cgcgccaatc accagacgcg ccgttaacat gctgccatct
      841 ttcagcgtca ggaaagtttc attttctccc caggcgacct gctgtaattc tgcgggggcc
      901 aacagagtga tatctgacga ctgctgcgct ttgttccaca gcgcgtagtg aatcactgag
      961 ttttcaacga tatgcccaag atggctatag cccatgcttt gatcgtcaaa cgaaatgtga
     1021 ccaaaactgt ctttgtccca cacttccata ccgtgataac aactggccct acgagagaga
     1081 atgtcctgcc agacgccaag acgggtgagt aatttttcgc tggcggcatt gatagccgaa
     1141 acgcgcagtt gtggtggtgc atccgccgcc agaggttccg gtacgcgctg ctccagtacg
     1201 gcaacgcgta agccgctccc ctgtaagccg caggcaaccg ccagccccac catgccgcca
     1261 ccaacaatgg ctacatcaac actttgcatt gtttattcct taaaaccgcc ttcaacgcgc
     1321 cacccaaccg agggtgcgct gcgccagcac atcgcgtgcc ggggtgaata attccatcgt
     1381 catcagtccg atgttgcgcc cgacaaccag cggtgcccaa cggttggcaa aaagatgtac
     1441 caggctgtcc gtgacgccga tggttgcttc gcgatcgctc tgtcgacgct gctgatagcg
     1501 gcacaatatg ccgtaatcac cgatgtcttc tccgcgctca tgcgcctgag tcagggtttc
     1561 cgcaaggctc atgacatctc gcataccgag gttaaaccct tgcccggcaa tcgggtgcag
     1621 agtttgcgcc gcattgccca ccagcacggt acgatgggta atagctctgg cggcgcgggt
     1681 taacgccagc ggataagcac tgcgtttacc ggcgtgggta atttttccaa gtcgccagcc
     1741 aaaggccgac tggagttcac ggcaaaactt ctcgtcgctc cacgacagca cctcttcgcg
     1801 ccgttccagt ggatgacacc agaccagcga acagcgtccg tcagacatcg gcaacatcgc
     1861 cagcgggcca tgttgcgtga agcgttcaaa agcgcggcct tcgtgcgcaa cggaagtcgc
     1921 aacgttggca atcacggcca gttgttcgta aggctcctgc tgccagtcaa cgccgcacgc
     1981 ggtggctaac gctgaatggg tgccatcagc tgctaccagc acgcggcccg tcagcgtctc
     2041 gccactctcc agcgtcactt caacgtgact ctgagtacgg gcaacgttag ccacgcgatc
     2101 agggcaatgc agcgttacgc caggtgcttt acgcagcaat gcaaacagcc gttgcccgac
     2161 attgtgcaat tcgacaacct gtcccagcgc cgccagttgg taatcttctg cggcgagggt
     2221 gacaaatcca gcgtgaccac gatcgctgac atgcacggta gtgatggcgg ttgcgtaatc
     2281 cgccagagat tgccagacgc cgatgcgcgc cagttgctga caggtccccg cagccagcgc
     2341 aatagctcgt ccatcaaagc ccggatgagc atgtgactct ggcgcggtcg cttcaatcaa
     2401 atgtaccggc agcgccccgt gacttaaccg ggaaatagcc agcgccagcg tcgcgcccgc
     2461 catgccgcca ccgacgatga ttacgctcat tgctttctcg cagctgccat caacgcttcg
     2521 atttcttccg gctttttcac cacgctggcg gtgaggtttt cgttgccggt ttcggtaatc
     2581 acaatgtcgt cttcaatacg aatgccgata ccgcgatatt gttctggcac atccgcatcc
     2641 ggcgcgatat acagaccagg ctctacggtc agtaccatcc ccggttccag aatgcgcgag
     2701 cgatcctgac cataaacgcc aacgtcatgg acatccagtc ctaaccagtg gctaaggcca
     2761 tgcataaaga aaggacgatg ggcgttctga gcgatcagtt catcaacatc acctttcagg
     2821 atgccgagtt ttaccaggcc gctaaccatg atgcgcacca cttcaccagt gacttccaga
     2881 atggaagttc ccggacgata caggcgcagg ctggtttcga gagactccag cacaatgtcg
     2941 tagatttcac gctgggcctg ggtgaatttg ccgttgaccg ggaaggtgcg ggtaatatcg
     3001 cccgcgtaac ctttgtattc acaacccgcg tcaatcaaca ccaggtcacc gtcgcgcagt
     3061 tcactctcgt tttcggtgta gtgcagaatg cagccgtttt caccgctgcc gacaatggtg
     3121 ttataggacg gatagcgcgc accgtggcgg ttaaattcgt ggtgaatttc gccttccaga
     3181 tggtactcga acattcccgg acggcatttt tccatcgccc gtgtatgtgc catggcggtg
     3241 atttctcccg cgcggcggag tacggcaatc tcttctggcg atttgaacag gcgcatttca
     3301 tgaacaacag gacgccagtc gatcatcgtt gccggtgcgg tgagattttg ccgcgaacct
     3361 ttacgcagtt tttccagcgc actgttcatg atttcatcag catatgcata ttcgccctgg
     3421 gcatggtaaa ccacatccag gccgttaagt agttgataaa gttgctgatt gatttcgctg
     3481 aatgccagtg cgcggtcaac gcccagtttc tctggcgcgg catcctggcc taagcgacgg
     3541 ccaaaccaga tctccgccgt caggtcgcga acgcggttaa acagaacgct gtggttatga
     3601 gtgtcatcgc ttttaatcag caccagcacc gcttccgatt cgttaaagcc ggtgaagtac
     3661 cagaagtcac tgttctgacg ataggggtat tcgctgtcgg cgctacgtgt tacttctggt
     3721 gcagcaaaaa tcagcgcggc gctgccgggt tgcatttgct ccaccagggc ctgacggcga
     3781 cgctgaaact cttgccggga tatctcactc ataacactct ccttacgttt tttgttttta
     3841 gtgtagagtc ggtttttgta cttctggcgc ggtcggttgc ggatgagtaa aggtgtcgtg
     3901 gcataacagc gcggcaacac gaacgtattc gatgatctct tcaagcgaca tttcaagctc
     3961 ttcctgatct tcgtcttcgt cgtaacccag ttgcgcaatg ttacgcagat cgtcgatagc
     4021 ttcaccggtt tcgccggtca ctttatccag cttcggttgc gtaacgccaa gaccaagcag
     4081 gaagtgattg acccaacctg ccaatgcatc agcccgatcg aaaacgctga catcatcgcc
     4141 atcaggcaga taaagctgaa aaaggaagcc gtcatcctgc agggcatcgc tggtggcaga
     4201 gtgcatttta cgcagtgcct gtgccagctc atgaccgaaa gccatgcctt cgttcgtcag
     4261 gtcgtgaagt agcggcagcc atgagctgtc atcgttaccg ccacatatca tcccactgat
     4321 taaaccatgc atctcggcag gggtcagccc cgtcccttgt tggttcagat actggttcat
     4381 ttcgttgtaa ccaggcattt cgttctgtat agacataagc attcgtcgtc aaagggagga
     4441 atattcatga tatgctacca ctttgggccc tggtggacca gaaaagggct tgtctcttct
     4501 catcagggta gctatagtgt cgccccttcg cagaccatgg gtctaaagac gaaggcagcg
     4561 cagtcaatca gcaggaaggt ggcatgtctg cacaacccgt cgatatccaa atttttggcc
     4621 gttcactgcg tgtgaactgc ccgcctgacc aaagggatgc gttgaatcag gcagcggacg
     4681 atctgaacca acggttgcaa gatctgaaag aacgcactag agtcacaaat actgaacagt
     4741 tggtcttcat tgccgcattg aatatcagct atgagttagc gcaagaaaaa gcgaagactc
     4801 gtgactacgc ggcaagtatg gaacagcgta ttcggatgct gcagcagacc atagaacaag
     4861 cgttacttga acaaggtcgc atcaccgaaa aaactaacca aaactttgaa tgacactttt
     4921 cggtttactg tggtagagta accgtgaaga caaaatttct ctgagatgtt cgcaagcggg
     4981 ccagtcccct gagccgatat ttcataccac aagaatgtgg cgctccgcgg ttggtgagca
     5041 tgctcggtcc gtccgagaag ccttaaaact gcgacgacac attcaccttg aaccaagggt
     5101 tcaagggtta cagcctgcgg cggcatctcg gagattccct tcttatctgg caccagccat
     5161 gacgcaacta ccagaactcc cactgacatt atcccgacaa gaaatccgca aaatgattcg
     5221 gcaacgtcgt cgtgcgttaa cgccggaaca acagcaggaa atgggtcaac aagccgctac
     5281 ccggatgatg acttatcccc cggtggtgat ggcgcatacg gtcgctgtat tcctctcttt
     5341 tgatggtgaa ctcgacaccc agccgctaat agaacaactc tggcgcgccg gtaagcgcgt
     5401 atatcttcca gttttgcatc cctttagtgc cggtaatttg ctgttcctga attaccatcc
     5461 gcaaagcgaa ctggtgatga acaggttgaa gatccatgag ccaaaactgg atgtgcgtga
     5521 cgtgctaccc ctttcccgat tagacgtgct gatcacaccg ctggtcgcct ttgatgagta
     5581 cggtcagcgc ctgggaatgg gcggtggttt ttatgatcgg accttacaaa actggcagca
     5641 ctataaaacg caaccggtgg gttatgcgca tgattgtcag ttggtggaaa aactccccgt
     5701 tgaagagtgg gatatccctc ttcctgcggt ggttacaccg tcgaaagtct gggagtggta
     5761 agggcgatac acctgcatcg ccctgattga catcgttgat tctttaacct aatttagtga
     5821 gtaagggtaa gggaggattg ctcctcccct gtgactgact gttaataagc tgggaaactt
     5881 atgagtaaca gtacaatcag tatgatggca agtcgcatca taacccttct ccttcaagcc
     5941 ctcgcttcgg tgagggcttt accgttacag ccccatgctg cccagctatc gtaagctctc
     6001 tttaaataac cacaacgcat tgatctacct ttaaatcttt tcttcgagcg tgctcgaaaa
     6061 acccatgcaa aacagagata atttttggga aaaaggatct attacggcag taaacgtggg
     6121 cgatgaagtg gctatcaaaa agttcggttc agggcgatgc accccgcatc gccctgattg
     6181 acatcgttga ttctttgacc taatttagtg agtaagggta agagaggatg actcctcccc
     6241 tgagactgac tgttaataag ctccgaaact tatcagtaac agtacaatca gtatgatgac
     6301 aagtcgcatc ataacccttc tcctttaagc cctcgcttcg gtgagggctt taccgttaca
     6361 gccccatgct gtcccgccat cgtaaatccc cattaaataa acacaacgca ttgatctgag
     6421 tttgatttat tttctggagc agactcgcaa agtagaatgc gcaacgcggc aacggtgtgg
     6481 agaagggata aaaaaacggg caagtcagtg acctgcccgt taattttcag agaaggggaa
     6541 ttagtacagc agacgggcgc gaatggtacc cggaatagct ttcattgcct gcagcgcttt
     6601 ttcggcaacg tcttcgtcag cttcaatatc aataactaca taacccatct gggcggaagt
     6661 ttgtagatat tgcgcggcga tgttgacgcc ctgctcggcg aagattttgt tcagcgcagt
     6721 tagcacgccc ggacggtttt cgtggatgtg catcagacga cgaccaccat gcagtggcag
     6781 cgagacttcc gggaagttca ccgcagagag cgttgagcca ttgtcagaat acttgatcaa
     6841 tttacccgca acttccaggc cgatattctc ctgcgcttcc tgagtcgaac cgccaatgtg
     6901 tggcgtcaga aggacgttgt cgaattcaca cagtggagag gtaaatggat cgctattggt
     6961 cgccggttcc gtcgggaata cgtcgattgc cgcccccgcc agatgtttgc tcgccagcgc
     7021 atcacacagc gccggaatat ccaccacagt accgcgcgag gcattaatca gcagcgagcc
     7081 gggcttcatt agcgaaatct ctttcgcgcc catcatattt ttggtggacg gattctctgg
     7141 tacatgcaga ctcaccacat cgctcatatt cagcaggtca gaaagatgct gtacctgagt
     7201 ggcgttgccc agcggcagtt tgttttcaat atcataaaag taaacataca ttcccagcga
     7261 ttcagccaga atgcccaatt gcgtaccaat atgaccgtag ccgatgatac ccagcttttt
     7321 gccgcgcgct tcaaaagaac ccgccgccag tttgttccac acgccacggt gcgctttagc
     7381 attggcttcc ggcacgccgc gcaatagcag cagcagttcg ccaatcacca gctccgcaac
     7441 agagcgcgta tttgagaacg gtgcgttaaa taccgggatc ccgcgctttg ccgccgcatc
     7501 cagatcaacc tggtttgttc cgatacagaa acagccaata gcgaccagtt tttctgcggc
     7561 gttgatcacg tcttcagtca gatgggtacg ggatcgcagg ccgatgaagt gggcatcgcg
     7621 gatggattct tttaattgtt catcatccag cgcgcctttg tgaaattcga tgttggtgta
     7681 accagctgca cgaaggcttt ccagcgcctt ttggtgcacg ccttctacca gcagaaactt
     7741 aatcttgtct ttctccagcg atacctttgc catttaccca atcctgtctt ttgaaatgtt
     7801 gtgtgcggat ttgcatccgc ctttcaacat atcaaaaaat aatattgcgg caatatgaac
     7861 gtttgcgtcg cgatgttgag gaaatatcac gcaaaggtat ttcttggttg aatatgctgg
     7921 tttagaggat ttatttagag caatcgacaa tagccaagta aaaacgtgac acatgtcacc
     7981 aaatttaatg aagagaattt ttttaacggg ggaggttccc ccgtcagatc atttcacaat
     8041 ggttttgaca ccgtcaggtg tgccaatcag cgcaacgtcc gcgccacggt tagcaaacaa
     8101 gccaacagtc accacgccag gaatcgcatt tatggcgttt tccatcgcta tcgggtcaag
     8161 gatttccatg ccgtggacgt cgaggatcac gttgccgtta tcggtcacca cgccctgacg
     8221 gtattccgga cgaccgccca gtttcaccag ctggcgcgcc actgcactac gtgccatcgg
     8281 gataacttct actggcagcg ggaatttgcc cagaatatca acctgcttgg aagcgtcagc
     8341 aatacagata aatttttccg caaccgaagc aatgattttt tcacgggtca gcgccgcgcc
     8401 gccgcctttg atcatttgca tgtggccgtt gatttcatct gcgccatcaa cgtagatgcc
     8461 aaggctgtcg acttcgttga gatcaaaaac gtgaatgccg aggcttttca gtttttcagt
     8521 ggaagcatct gaactggaaa cggccccttc aatctggcct ttcattgtac cgagcgcgtc
     8581 aataaagtgt gcggcggtgg aacctgtacc tacaccaaca atggtgccgg gctgaacata
     8641 ctgaagtgcc gcccatccta ctgctttttt caattcatcc tgcgtcatga tcgtttcgcc
     8701 tgtggtatga aatttcacac gcattatata caaaaaaagc gattcagacc ccgttggcaa
     8761 gccgcgtggt taactcatcc ataaaatatc gcgcaatggc gggcatcccc tttcgccccg
     8821 caaataaagc atacaacggt ctgggtatgc cgctccacgg tgcgaacagg cgcaccagtt
     8881 ccccccgttc gcaagcccct gtttacaggc aaattgaggc aataacgcca ccccattcaa
     8941 cacaaccagg gcacgaactc gctgagcgaa atgcattagc gcaaatccct gatcttttaa
     9001 gctgcgcgtt taaccacgga tacaaatgtt acccgccgac ggcttcggta tatgcaacct
     9061 gacacaaaat tgtgtcatag tgcaggaaaa agcatttacc aggagcagac aacagcaatg
     9121 aaacgcccgg actacagaac attacaggca ctggatgcgg tgatacgtga acgaggattt
     9181 gagcgcgcgg cacaaaagct gtgcattaca caatcagccg tctcacagcg cattaagcaa
     9241 ctggaaaata tgttcgggca gccgctgttg gtgcgtaccg taccgccgcg cccgacggaa
     9301 caagggcaaa aactgctggc actgctgcgc caggtggagt tgctggaaga agagtggctg
     9361 ggcgatgaac aaaccggttc gactccgctg ctgctttcac tggcggtcaa cgccgacagt
     9421 ctggcgacgt ggttgcttcc ggcactggct cctgtgttgg ctgattcgcc tatccgcctc
     9481 aacttgcagg tagaagatga aacccgtact caggaacgtc tgcgccgcgg cgaagtggtc
     9541 ggcgcggtga gtattcaaca tcaggcgctg ccgagttgtc ttgtcgataa acttggtgcg
     9601 ctcgactatc tgttcgtcag ctcaaaaccc tttgccgaaa aatatttccc taacggcgta
     9661 acgcgttcgg cattactgaa agcacctgtg gtcgcctttg accatcttga cgatatgcac
     9721 caggcctttt tgcagcaaaa cttcgatctg cctccaggca gcgtgccctg ccatatcgtt
     9781 aattcttcag aagcgttcgt gcaacttgct cgccagggca ccacctgctg tatgatcccg
     9841 cacctgcaaa tcgagaaaga actagccagc ggtgaactga ttgacttaac gccagggcta
     9901 tttcaacgcc ggatgctcta ctggcaccgc tttgctcctg aaagccgcat gatgcgtaaa
     9961 gtcactgatg cgttgctcga ttatggtcac aaagtccttc gtcaggatta atccatcaaa
    10021 taatgcctga tagcacatat caggcgttat cctcacttct ttttgtattc cttgaatcac
    10081 atcacaaaat agacaaatct caggcggcaa aaaacgacgt ctgaatgcat ttttttgctg
    10141 gcgacaaacc cacgtaaaaa gctcaccgta agcgcaaata ccctcatttt gattgcgttt
    10201 tacggagcaa ataatgtcta acgagcagga gtggcaacag cttgccaaca aggaattgag
    10261 ccgtcgggag aaaactgtcg actcgctggt tcagcaaacc gcggaaggga tcgccatcaa
    10321 gcagctgtat accgaagccg atctcgataa tctggaggtg acaggtaccc ttcctggttt
    10381 gccgccctac gttcgtggcc cgcgtgccac tatgtatacc gcccaaccgt ggaccatccg
    10441 tcagtatgct ggtttttcaa cagcaaaaga gtccaacgct ttttatcgcc gtaacctggc
    10501 cgccgggcaa aaaggtcttt ccgttgcgtt tgaccttgcc acccaccgtg gctacgactc
    10561 cgataaccca cgcgtggcgg gtgacgtcgg caaagcgggc gtcgctatcg acaccgtgga
    10621 agatatgaaa gtcctgttcg accagatccc gctggataaa atgtcggttt cgatgaccat
    10681 gaatggcgca gtgctaccag tactggcgtt ttatatcgtc gccgcagaag agcaaggtgt
    10741 tacacctgat aaactgaccg gcaccattca aaacgatatt ctcaaagagt acctctgccg
    10801 caacacctat atttacccac caaaaccgtc aatgcgcatt atcgccgaca tcatcgcctg
    10861 gtgttccggc aacatgccgc gatttaatac catcagtatc agcggttacc acatggggga
    10921 agcgggtgcc aactgcgtgc agcaggtagc atttacgctc gccgatggga ttgagtacat
    10981 caaagcagca atctctgccg gactgaaaat tgatgacttc gctcctcgcc tgtcgttctt
    11041 cttcggtatc ggcatggatc tgtttatgaa cgtcgccatg ttgcgtgcgg cacgttattt
    11101 atggagcgaa gcggtcagtg gatttggcgc acaggatccg aaatcactgg cgctgcgtac
    11161 ccactgccag acctcaggct ggagcctgac tgaacaggat ccgtataaca acgttatccg
    11221 caccaccatt gaagcactgg ctgcgacgct gggcggtact cagtcactgc ataccaacgc
    11281 ctttgacgaa gcgcttggtt tgcctaccga tttctcagca cgcattgccc gcaacaccca
    11341 gatcatcatc caggaagaat cagaactctg ccgcaccgtc gatccactgg ccggatccta
    11401 ttacattgag tcgctgaccg atcaaatcgt caaacaagcc agagctatta tccaacagat
    11461 cgacgaagcc ggtggcatgg cgaaagcgat cgaagcaggt ctgccaaaac gaatgatcga
    11521 agaggcctca gcgcgcgaac agtcgctgat cgaccaaggc aagcgtgtca tcgttggtgt
    11581 caacaagtac aaactggatc acgaagacga aaccgatgta cttgagatcg acaacgtgat
    11641 ggtgcgtaac gagcaaattg cttcgctgga acgcattcgc gccacccgtg atgatgccgc
    11701 cgtaaccgcc gcgttgaacg ccctgactca cgccgcacag cataacgaaa acctgctggc
    11761 tgccgctgtt aatgccgctc gcgttcgcgc caccctgggt gaaatttccg atgcgctgga
    11821 agtcgctttc gaccgttatc tggtgccaag ccagtgtgtt accggcgtga ttgcgcaaag
    11881 ctatcatcag tctgagaaat cggcctccga gttcgatgcc attgttgcgc aaacggagca
    11941 gttccttgcc gacaatggtc gtcgcccgcg cattctgatc gctaagatgg gccaggatgg
    12001 acacgatcgc ggcgcgaaag tgatcgccag cgcctattcc gatctcggtt tcgacgtaga
    12061 tttaagcccg atgttctcta cacctgaaga gatcgcccgc ctggccgtag aaaacgacgt
    12121 tcacgtagtg ggcgcatcct cactggctgc cgatcataaa acgctgatcc cggaactggt
    12181 cgaagcgctg aaaaaatggg gacgcgaaga tatctgcgtg gtcgcgggtg gcgtcattcc
    12241 gccgcaggat tacgccttcc tgcaagagcg cggcgtggcg gcgatttatg gtccaggtac
    12301 acctatgctc gacagtgtgc gcgacgtact gaatctgata agccagcatc atgattaatg
    12361 aagccacgct ggcagaaagt attcgccgct tacgtcaggg tgagcgtgcc acgctcgccc
    12421 aggccatgac gctggtggaa agccgtcacc cacgtcatca ggcgctaagt acacagctgc
    12481 ttgacgccat tatgccgttc tgtggtaacg ccctgcgact gggcgttacc ggcacacccg
    12541 gcgcggggaa aagtaccttt cttgaagcct ttggcatgtt gttgattcgt gagggattaa
    12601 aggttgctgt tattgcggtc gatcccagca gcccggtcac tggcggtagc attctcgggg
    12661 ataaaacccg catgaatgac ctggcgcgtg ccgaggcggc gtttattcgc ccggtaccct
    12721 cctccggtca tctgggcggt gccagtcagc gagcacggga attaatgctg ttatgcgaag
    12781 cggcgggtta tgacgtagtg attgtcgaaa cggttggcgt cgggcagtcg gaaacggaag
    12841 tcgcccgcat ggtggactgt tttatctcgt tgcaaattgc cggtggcggc gatgatctgc
    12901 agggcattaa aaaagggctg atggaagtgg ctgatctgct cgttatcaac aaagacgatg
    12961 gcgataacca taccaatgtc gccattgccc ggcatatgta cgagagtgcc ctgcatattc
    13021 tgcgacgtaa atacgacgaa tggcagccac gggttctgac ttgtagcgca ctggaaaaac
    13081 gtggaatcga tgagatctgg cacgccatca tcgacttcaa aaccgcgcta actgccagtg
    13141 gtcgtttaca acaagtgcgg caacaacaat cggtggaatg gctgcgtaag cagaccgaag
    13201 aagaagtact gaatcacctg ttcgcgaatg aagatttcga tcgctattac cgccagacgc
    13261 ttttagcggt caaaaacaat acgctctcac cgcgcaccgg cctgcggcag ctcagtgaat
    13321 ttatccagac gcaatatttt gattaaagga atttttatgt cttatcagta tgtgaacgtt
    13381 gccactatca acaaagtggc ggtcattgag tttaactatg gacgaaaact taatgcctta
    13441 agtaaagtct ttattgatga tcttatgcag gcgttaagcg atctcaaccg gccggaaatt
    13501 cgctgtatca ttttgcgggc gccaagtgga tccaaagtct tctccgcagg tcacgatatt
    13561 cacgaactgc cgtctggcgg tcgcgatccg ctctcctatg atgatccatt gcgtcaaatc
    13621 acccgcatga tccaaaaatt cccgaaaccg atcatttcga tggtggaagg tagtgtttgg
    13681 ggtggcgcat ttgaaatgat catgagttcc gatctgatca tcgccgccag tacctcaacc
    13741 ttctcaatga tgcctgtaaa cctcggcgtc ccgtataacc tggtcggcat tcacaacctg
    13801 acccgcgacg cgggcttcca cattgtcaaa gagctgattt ttaccgcttc gccaatcacc
    13861 gcccagcgcg cgttggctgt cggcatcctc aaccatgttg tggaagtgga agaactggaa
    13921 gatttcacct tacaaatggc gcaccacatc tctgagaaag cgccgttagc cattgctgtt
    13981 atcaaagaag agctgcgtgt actgggcgaa gcgcacacca tgaactccga tgaatttgaa
    14041 cgtattcagg ggatgcgccg cgcggtttat gacagcgaag attaccagga agggatgaac
    14101 gctttcctcg aaaaacgtaa acctaatttc gttggtcatt aatccctgcg aacgaaggag
    14161 taaaaatgga aactcagtgg acaaggatga ccgccgatga agcggcagaa attatccagc
    14221 ataacgacat ggtggcattt agcggcttta ccccagcggg ttcgccgaaa gccttaccga
    14281 ccgcgattgc ccgcagagct aacgaacagc atgaggccaa aaagccgtat caaattcgcc
    14341 tgctgacggg ggcgtcaatc agcgccgccg ctgacgatgt actttctgac gccgatgctg
    14401 tttcctggcg tgcgccatat caaacatcgt ccggtttacg taaaaagatc aatcagggcg
    14461 cggtgagttt cgttgacctg catttgagcg aagtggcgca aatggtcaat tacggtttct
    14521 tcggcgacat tgatgttgcc gtcatcgaag catcggcact ggcaccggat ggtcgagtct
    14581 ggttaaccag cgggatcggt aatgcgccga catggctgct gcgggcgaag aaagtgatca
    14641 ttgaacttaa tcactatcac gatccgcgcg ttgcagaact ggcggatatt gtgattcctg
    14701 gcgcaccacc gcggcgcaat agcgtgtcga tcttccatgc aatggatcgc gtcggtaccc
    14761 gctatgtgca aatcgatccg aaaaagattg tcgccgtcgt ggaaaccaac ttgcccgacg
    14821 ccggtaatat gctggataag caaaatccca tgtgccagca gattgccgat aacgtggtca
    14881 cgttcttatt gcaggaaatg gcgcatgggc gtattccgcc ggaatttctg ccgctgcaaa
    14941 gtggcgtggg caatatcaat aatgcggtaa tggcgcgtct gggggaaaac ccggaaattc
    15001 ctccgttcat gatgtattcg gaagtgctac aggaatcggt ggtgcattta ctggaaaccg
    15061 gcaaaatcag cggggccagc gcctccagcc tgacaatctc ggccgattcc ctgcgcaaga
    15121 tttacgacaa tatggattac tttgccagcc gcattgtgtt gcgtccgcag gagatttcca
    15181 ataacccgga aatcatccgt cgtctgggcg tcatcgctct gaacgtcggc ctggagtttg
    15241 atatttacgg gcatgcaaat tcaacgcacg tagccggggt cgatctgatg aacggcatcg
    15301 gcggcagcgg tgattttgaa cgcaacgcgt atctgtcgat ctttatggct ccgtcgattg
    15361 ctaaagaagg caagatctca accgtcgtgc caatgtgcag ccatgttgat cacagcgaac
    15421 acagcgtcaa agtgatcatc accgaacaag ggatcgccga tctacgcggt ctttccccgc
    15481 ttcaacgcgc ccgcactatc attgataatt gtgcacatcc tatgtatcgg gattatctgc
    15541 atcgctatct ggaaaatgcg cccggcggac atattcacca cgatcttagc cacgtcttcg
    15601 acttacaccg taatttaatt gcaaccggct cgatgctggg ttaatggttt gtcccgtcac
    15661 gatcttcagc aatatgctga agcatcgtgg cggtataacg atgatttctg attgaataaa
    15721 gatattcctg catatacatc ggcctgtgcg gcgcatcaaa atatttcagc gttgccggat
    15781 tcaccagtcg ccaggcaaaa tgcgggatca ccgtcagaaa atgtccgcgc tccacggcgc
    15841 tgattttggc cataaaactg tagggacgat aaataatggt gggattgatc ccacaaggtc
    15901 gcatattcgc atcaatcatt gcttcgaagt tagcccggtt ttgaaagcgc atttgtagcc
    15961 agggcagctc ctgaagcagc tcttgctctt gacgctcttc ataacggcga gaaacgagaa
    16021 agcccaatcg cagcggtggt aattcactga tagtcaggtt ttccagttcc tgaactcgtg
    16081 cagatacatg ctgtggggag ataataaaat ctaactgacg gtcaaaaaga ttatcgataa
    16141 cgccgttttc gctgaactca actggctggg cggtcacgcc ttcatactta tctccgagac
    16201 taatcaactg atcgaaaata attgtcggat aagtattatc tatgccaatc acaatt
//
LOCUS       DAZOZY010000082        16208 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZOZY010000082 DAZOZY010000000
VERSION     DAZOZY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16208
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.082"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHURQ_002297"
     CDS             complement(<1..81)
                     /locus_tag="ACHURQ_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR0824057.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHURQ_002298"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHURQ_002298"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR0824058.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHURQ_002299"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHURQ_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR0824059.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHURQ_002300"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHURQ_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR0824060.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHURQ_002301"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHURQ_002301"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR0824061.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHURQ_002302"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHURQ_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR0824062.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHURQ_002303"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHURQ_002303"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR0824063.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHURQ_002304"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHURQ_002304"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR0824064.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHURQ_002305"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHURQ_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHURQ_002306"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHURQ_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR0824065.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHURQ_002307"
     CDS             complement(12387..12626)
                     /locus_tag="ACHURQ_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR0824066.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHURQ_002308"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHURQ_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR0824067.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHURQ_002309"
     CDS             13003..13290
                     /locus_tag="ACHURQ_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR0824068.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13960..14103
                     /locus_tag="ACHURQ_002310"
     CDS             13960..14103
                     /locus_tag="ACHURQ_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR0824069.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14116..14325
                     /gene="cspE"
                     /locus_tag="ACHURQ_002311"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14116..14325
                     /gene="cspE"
                     /locus_tag="ACHURQ_002311"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0824070.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHURQ_002312"
     CDS             14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHURQ_002312"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR0824071.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHURQ_002313"
     CDS             complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHURQ_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR0824072.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4241 a         3937 c         4108 g         3922 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc
    13561 ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat tgatagccgt
    13621 taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat
    13681 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
    13741 gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca
    13801 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
    13861 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
    13921 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
    13981 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
    14041 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
    14101 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
    14161 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct
    14221 atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
    14281 gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
    14341 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
    14401 atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
    14461 tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag
    14521 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
    14581 aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga tccgggcgac
    14641 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
    14701 cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg
    14761 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc
    14821 accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg
    14881 caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac
    14941 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc
    15001 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
    15061 atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta
    15121 cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
    15181 ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac actggcagca
    15241 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
    15301 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga
    15361 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt
    15421 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
    15481 aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
    15541 agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa
    15601 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc
    15661 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg
    15721 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
    15781 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
    15841 agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca
    15901 tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca
    15961 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc
    16021 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
    16081 ccaaatgagg gcgcaacctt aacatatttt gagaatataa aacaactgtt ggaatatatt
    16141 atttcattgc tttattgata acgattttca tttggattta tatatcaatg aaacgttaaa
    16201 ataaataa
//
LOCUS       DAZOZY010000083        16058 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZOZY010000083 DAZOZY010000000
VERSION     DAZOZY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16058
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.083"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(141..293)
                     /locus_tag="ACHURQ_002314"
     CDS             complement(141..293)
                     /locus_tag="ACHURQ_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR0824073.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(558..1292)
                     /gene="cysH"
                     /locus_tag="ACHURQ_002315"
     CDS             complement(558..1292)
                     /gene="cysH"
                     /locus_tag="ACHURQ_002315"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR0824074.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1367..3079)
                     /gene="cysI"
                     /locus_tag="ACHURQ_002316"
     CDS             complement(1367..3079)
                     /gene="cysI"
                     /locus_tag="ACHURQ_002316"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR0824075.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3079..4878)
                     /gene="cysJ"
                     /locus_tag="ACHURQ_002317"
     CDS             complement(3079..4878)
                     /gene="cysJ"
                     /locus_tag="ACHURQ_002317"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR0824076.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5197..5559
                     /gene="queD"
                     /locus_tag="ACHURQ_002318"
     CDS             5197..5559
                     /gene="queD"
                     /locus_tag="ACHURQ_002318"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR0824077.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5637..6908
                     /locus_tag="ACHURQ_002319"
     CDS             5637..6908
                     /locus_tag="ACHURQ_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0824078.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6899..7159
                     /locus_tag="ACHURQ_002320"
     CDS             6899..7159
                     /locus_tag="ACHURQ_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0824079.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7176..7751
                     /locus_tag="ACHURQ_002321"
     CDS             7176..7751
                     /locus_tag="ACHURQ_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR0824080.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7899..8759)
                     /locus_tag="ACHURQ_002322"
     CDS             complement(7899..8759)
                     /locus_tag="ACHURQ_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR0824081.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8756..9535)
                     /locus_tag="ACHURQ_002323"
     CDS             complement(8756..9535)
                     /locus_tag="ACHURQ_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR0824082.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9513..10463)
                     /locus_tag="ACHURQ_002324"
                     /pseudo
     CDS             complement(9513..10463)
                     /locus_tag="ACHURQ_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10450..11883)
                     /locus_tag="ACHURQ_002325"
     CDS             complement(10450..11883)
                     /locus_tag="ACHURQ_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR0824083.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11953..12769)
                     /locus_tag="ACHURQ_002326"
                     /pseudo
     CDS             complement(11953..12769)
                     /locus_tag="ACHURQ_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13087..14364
                     /locus_tag="ACHURQ_002327"
     CDS             13087..14364
                     /locus_tag="ACHURQ_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR0824084.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14391..15877
                     /locus_tag="ACHURQ_002328"
                     /pseudo
     CDS             14391..15877
                     /locus_tag="ACHURQ_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3791 a         4228 c         4039 g         4000 t
ORIGIN      
        1 taatcaaata attttcatcc gtaatatata tgaataataa acataggcat aatattcaca
       61 taaataatat aaattaaaaa agggtgcagg agaccaaacc atcacaattc agtacgcggg
      121 gttttacccc cgcgtggtgg ctacttcttc gattcgtaag cgagaactgc cttaaactcc
      181 ataccacgtt ctctgatact caactcacac aacgagtcgc ctaccatcag cgtgaatccc
      241 agtactgtaa aacacagcac tatgattgcc acaagggcat attttgtcag catgtcttgc
      301 ctccctgcaa agaagagact aacattcgaa ttgtttgggt tcgaacgctg gcctcaggtt
      361 gatagaaata tcgcctgggg cttttgtcca tctggaacct cgcgaatgct taacgccaga
      421 cagcctcaag cacccgacgc cattctatac ctgataattc ttccagcggc gttcacgttt
      481 aaaatcagga atactgctcg caaaataaaa acgcccccat catttctgac agaggcgttt
      541 aatttatccg gcaatattta cccttcgtgc aatccacatt ccctttttaa gccaaagaaa
      601 cgtgtttctt cttccgacat gccaggttcc catttacggg ttgtatgggt atcaccgacc
      661 gataaatatc cttcatccca taatgggtga tatttcaggc catgtttttg caggtactga
      721 taaatagttc ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt
      781 gccagcaccg gtaaattggc gcgactgccg gattgttcac ggcgcaggcc agcaaaccag
      841 gtttgcgcgt taagttcttt cagagcccgg ttcatcggtt cgactttgtt gatgtcattg
      901 tacttttcaa tgccttcaac gccctgctcc cacagttttc cgtagcgtgc ttcctgccag
      961 gctgcgcttt cggtagcacg gtacactttc aggttgagct tgagtttgtc cgttaactcg
     1021 tcaataaagc ggtaggtttc cgggaacaag taacccgtat cggtgaggat caccggaata
     1081 tccgggcgaa tttgattcac cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa
     1141 gaaagtacat attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc
     1201 agtttttcca gttcggcgtt agtttccgcc agcgccagaa tacgatctac cttcggcagt
     1261 tcgttcaggg cgtttagatc gagtttggac atagattcct cactgtttgc cttgcctgat
     1321 gcgacgctca cgcgttctta tcaggcctac aagaccgggc tgatggttaa tcccacaaat
     1381 cgcgcgccgg atcgagcacc gggcgaatga tgcccgcacg caccgtaaag tcgccgaagc
     1441 cttcacccgc ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc gacgccagga
     1501 tttccggctc ggtgatgttt tctttataca tccgtgggat acgtgtccca atgcggttgc
     1561 cgccaagatg caggttgtag cgacccggcg ctttacccac caggcccact tccgccagca
     1621 tcgcgcgacc acaaccgttc gggcagcctg ttacacgcat cacgatatgc tcatcgctga
     1681 caccatgttt cgccattaaa ttatcgatgt tgtcgataaa agacggcagg aaacgctctg
     1741 cttccgccat cgccagcggg caagtcggga atgacacgca ggccatcgag ttttcacgct
     1801 gcggcgtgac ggcattcatt aacccgctct ctttggcgat cttctcgatc ttcgctttct
     1861 cgctttccgg tacaccggtg atgatcagat tctggttcgc cgtaatgcgg aaatcgcctt
     1921 tgtggatctt cgcgatctcc agcaggccgg ttttcagcgg acgccccgga taatcaagga
     1981 tgcgaccatt ttcgataaac agcgtcaggt gccagttatc atcaatgccc ttaacccagc
     2041 caatacgatc gcctcgtccg gtgaactcat atggacggat cggttcaaat ttgatccccg
     2101 cgcgacgctc cacttccgct ttaaacgtct caacccccac gcgctccagc gtgtatttgg
     2161 ttttggcatt tttacgatcg gttcggttac cccagtcacg ctgagttgtc acgacggctt
     2221 cggccaccgc cagcgtatgc tccagcggca gatagccaaa ctcgctcgcc gtgcgggcgt
     2281 aggttttctt gttgccgtgt tcgatagaaa gcccaccgcc caccagcagg ttgaagccta
     2341 ccagcttgcc gttttcggcg attgccacga agttcatgtc gttggcatgc agatcgatat
     2401 cgttctgcgg cgggatcact accgtggttt tgaacttacg cggcaggtag gtctgaccaa
     2461 ggatcggttc ttcatcagta gtggcgactt tttcctggtc gagccagatc tccgcatacg
     2521 cgcgggtacg aggcaacaga tgctcagaaa tcttcttcgc ccactcgtac gcttccgcgt
     2581 gcagctgcga ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg tcgttagctg
     2641 tcgccagcgc atcaagaccg accgagtgca gcatctggtg caccggtttg acgttctttt
     2701 tcagaatgcc gtggaactga aacgtctggc ggttggtcag gcgaatgctg ccatagatgg
     2761 tgttttcacc ggcaaatttg tcgatcgcct gccactgttt agtggtaatc accccacccg
     2821 gcagacgaca gcgaagcagc atcgcgtggc gcggctccag cttctgttca gcacgttcgg
     2881 cacggatgtc gcggtcatcc tgctgataca tgccgtggaa gcggatcagc aggaagttgt
     2941 cgcccttaaa gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg ccgcgcaggt
     3001 agttgctttc aagcttcatg cgctcggcgt ctgtcagttt tccttcgacc actaaaggcc
     3061 ctggatgttt ttcgctcatt agtagacatc tcgctgataa cggcgctcta cgcgcagctc
     3121 acttaaaaat tcatccgccg cttcggtgtc catgccacca aattcggcaa tcacttccag
     3181 aagtgcctgc tcaacgtctt tcgccatgcg attagcgtcg ccgcagacat aaatgtgggc
     3241 accgtcattg atccagcgcc acagctccgc gccctgttcg cgcagtttgt cttgtacata
     3301 aactttttct ttttggtcgc gcgaccaggc aagatcgata cgcgtcagca cgccctcttt
     3361 gacgtagcgc tgccactcca cctggtagag gaaatcttcc gtaaagtgcg ggttgccaaa
     3421 gaacagccag tttttacccg gcgcttcgtc ggcggcgcgt tgctgcataa aggcacggaa
     3481 cggcgcgatg ccggtaccgg ggccaatcat aatcactggc gtttgcgggt tagcgggcag
     3541 gcggaaattg tcgttatgtt cgataaatac gcggacttcg ccctcttctt ccacgcgatc
     3601 cgcgaggaag ctggaggcac caccggcacg ggcgcggcct tccacgtcgt aacgcaccac
     3661 accaacggtg acgtgtactt cgttctcgac ttccgcctgc gaggaggcga tggaatacag
     3721 gcgcggggtc agcgggcgca gcaggttaat cagcgcttcg gcatccagtt gcgccggaga
     3781 gaaacgcacc atgtcgacaa tcggcgtggt cgcggcgtaa tgctgtaact tcgctttatc
     3841 gcccaccagc ggcagcagcg tttcgctgcg ggtaagcgtg gcatagttct caacaatgtt
     3901 ggcggtgttg acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag gcaacgtttt
     3961 gccctcgacg gtgacaggtt catcgccttt cagccacagc agttcgacaa gttctttcac
     4021 cagtgccggg tcgttctgat accagacacc cagcgcgtca cccggctggt aacgcaggcc
     4081 tgaatcgcct aagtcaattt cgatatgacg aacgtctttt tcagagttac gcccggtaat
     4141 tttctggtta accgaaaggc tcgccaccag cggcgcgtct ttgctgtacg ggctggtgtg
     4201 gatttcattt accgcgccag tagcgacgga ttgcgaaggt gccgcgacag gcgcacgcga
     4261 tttaagcgca tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt attcaacatc
     4321 ggcatcgaca cggtcgagca ggcgttcacc acccagttcc gccagcttgc tgtcgaaatc
     4381 tttcccggac tggcagaaaa attcataaga gctatcgccg aggctaaaca cggcaaacgc
     4441 ggtgttttcc agctttggcg cttttttgga gaacaggaac ttatgcagcg cgacggcttc
     4501 ttccggcggt tccccttccc cttgcgttga cgtcactacg atgagcagtt tttcgctggc
     4561 gatttgtttg aatttatagt cgcccgcgtt caccagctta acgttcagtt ttgctgctaa
     4621 taaatcatcg cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg aggcggagat
     4681 aatagttata cccggcattt ctgcggctgg cgctggcgtc gctgcaagcg cagcaggctg
     4741 ctgattgagt acgccccaga aatagccaga aacccaggca agctgggtag gcgttaaatc
     4801 ggtcgtggcc gcctgaaggc gtgccagttg ctccgggttc aacggaagca acgcggaagg
     4861 tgggacctgt gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt ctgcgccctg
     4921 tcagcgccca taaaacagaa gagaagataa ggttaacggg gtgaacggtg tggattaaag
     4981 acgggatagc gataactaat aaccaaatcg actaacctgt tttagcaata gtctttaaca
     5041 acaaaataga ttaaccaacc caatgaaaaa caaatgaatt tagccaatca ttaagataaa
     5101 tcagcgattt tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc tttccggtac
     5161 tctacggcgg ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt atttaaagat
     5221 ttcaccttcg aagccgctca ccgcttacca cacgtcccgg aagggcataa atgtggtcgc
     5281 ctgcacgggc attcctttat ggtgcgactg gaaattaccg gggaagtcga tccgcatacg
     5341 ggctggatta tcgatttcgc tgaactgaaa gcggcgttta agccaactta tgagcgcctc
     5401 gatcaccatt atctcaatga tattccaggt ctggaaaacc caaccagcga agttttagca
     5461 aaatggattt gggatcaggt taaacccgtt gtgccgctgt taagtgcggt gatggtaaaa
     5521 gaaacctgca ccgcagggtg tatctatcgc ggcgaatgat aagagtgtgt cggcggtcaa
     5581 tttcccttaa gtaacgctat gttagggtgt tgtgttctgg atatctgggg catgacatgg
     5641 aagacgactg cgacattatt attattggtg ccggtattgc aggcaccgct tgcgcgttac
     5701 gctgcgcgcg agcgggttta tccgttttgt tactggaacg cgctgaaatc cccggcagca
     5761 aaaatctttc cggcgggcgg ttatataccc atgcactcgc ggaactcctc ccgcaatttc
     5821 atctgaccgc gcctcttgaa cgacgcatca ctcacgaaag cctttccctg ttaacgcccg
     5881 atggcgtaac gacgttttcc agcttacagc ccggcggtga atcctggagt gtattacgtg
     5941 cacggttcga tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa tgcatccccg
     6001 gagcgacggt ggatgcactg tatgaagaaa acggcagagt ctgtggcgtt atttgtggtg
     6061 acgatattct ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc gtcctggctg
     6121 aacgtcacgg gttagtgact cgtcctgctg gcgaagcgat ggcgttgggg atcaaagaag
     6181 tgctgtcgct ggaaacatcc gctattgaag aacgttttca tctggagaat aacgaaggcg
     6241 cagcgttgct gttcagcggc ggaatctgtg atgacttacc cggcggcgca tttctttata
     6301 ctaatcaaca aacgctctcg ttagggattg tttgcccgct ctcttccctt acgcaaagtc
     6361 gtgttccggc aagcgagctg ctgactcgct ttaaagcgca tccggcagtg cgcccgctta
     6421 tcaaaaacac ggaatcactg gagtatggtg cgcatctgga gccagaaggt ggcttgcaca
     6481 gtatgccggt gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg ttgcgcagtt
     6541 gcgtcaatac cggaatttcc gtgcgcggca tggatatggc gctgactggc gcacaggcgg
     6601 cggcacaaac gctaataagc gcctgccagc accgcgagcc gcaaaatctg tttgcgcttt
     6661 atcatcacaa cgtagagcgc agcctgctat gggatgttct acaacgttat cagcatgttc
     6721 cggcgctttt gcaacgccct ggctggtatc gggcgtggcc tgcgttaatg caggatattt
     6781 cccgcgattt atgggatcag ggtgataaac ctgttccacc gctgcgccag ttattctggc
     6841 gtcatttacg tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg agtctgcgat
     6901 gtctgtagcc cataatctct ggcgcgttgc tgatgcgccg cacattgttc cggctgactc
     6961 cgttgagcgt cagatggtac aacggttgat taacgcctgt ccggcaggtc ttttttcgct
     7021 cacaccggaa ggtgacttac gcgttgatta tcacggctgc ctggagtgtg gcacctgccg
     7081 tttgctgtgc gacgaatcca cactacaaca gtggcgctat ccgccttccg gattcggcat
     7141 cacctaccgc tttggataaa taataaggat aatttatgcc cctcttacac ctactccgcc
     7201 agaatccggt gattgctgcc gttaaagata atgccagcct gcaactggct atcgattctg
     7261 aatgccagtt tatttccgtg ttgtacggca atatctgcac catcagtaat atcgttaaaa
     7321 agattaagaa cgccgggaaa tatgctttta ttcatgttga tttgctggaa ggcgcgtcaa
     7381 ataaagaagt cgttattcag tttttgaaac tggtgaccga ggcggacggc attatcagta
     7441 ccaaagcctc aatgttgaaa gcggcaagag cggaaggttt tttctgtatt catcgcctgt
     7501 ttattgttga ttcaatttcg tttcacaaca ttgataagca agttgcgcaa tcgaatccgg
     7561 attgtattga gatcctgcca ggctgtatgc ccaaagtgct gggctgggtg acagagaaaa
     7621 tccgccaacc gctgattgcc ggtgggctgg tgtgcgatga agaagatgcg cgtaatgcga
     7681 ttaacgcggg tgtcgtggcg ctttccacca cgaataccgg ggtctggacg ttagcgaaaa
     7741 aattactttg acgggataat cgtaaccaat tgaatttggt ttgatttgta ggccgcacgc
     7801 cacatccgac attcagcgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaagtcc
     7861 ccaggccaga accgtaggca ggatcatccg gcaacacgtt attggcaatc tgcgtgaatg
     7921 tttgtcacca gtgcctccag caccactttc cagtcatcaa ccacgccaac atctgcctgt
     7981 gaaaataccg cagcgctggc gtcatgatta atcgccacca caaacttgct attgcgtacg
     8041 cccgccatta acgctgctgc gccagaagcg ccaacaacaa tacacacctc aggtgccagc
     8101 agatgtccgg aaataccaat cactttttca gcatcaacgc caccgttcat cactcgcgcc
     8161 cgactgtagc ccacttcggc tcccagcttt tctgccagca tagcgatttc ctggttatct
     8221 gcctctccgc cctgccccac caccagcacc cgtctggcct cagctaatgg gtcgcgagta
     8281 acatttttca gatcctctgt gctaaccagc cagtccggaa gcgcacccgg gacaatgttc
     8341 agttgttgca ttccagaggg caacgtggcg tttttcgctg ctcccgcctg acgcgccagg
     8401 gaaaggcata gcgggcgttt ttctgtttgc agcgtcgccg ttagcgcatt tccccagtgg
     8461 gatttgcgca cgcttacggt tggaatatct aacgaagtca cctggcagat gcttgcacca
     8521 tgtaaacgcc aggccagccg ggtcgacagt tcatcgccaa acgttcccgg cggaaagaga
     8581 acaacgtccg caggcgttcg ctgccactgt tccaccaacg catccagcac ctgttccgcc
     8641 actacaggct gaggttcaat ctgccagtgc gccagggtac aaccgctgaa atcctgcgcc
     8701 gccagccagc ttgctattgc ggcattctcc tgatttattg tcacaattgc gatattcatg
     8761 gctgcatcct ttggcgtaaa tagtcctgcc atagcttttg agctttttct gctaccgttt
     8821 gcccatcaat cagcgtcgct ccgcgtcgtt gctctgctct tgccagttgc agacactgca
     8881 tcgctggcat ttcggcagca acagttttac ggataatttc tgctttcccc gcagccattc
     8941 gctggcgcat tcccggtact ggcaaggcca cttcaccgca ctgacgcacg gcaatcaccg
     9001 caggcaagcg aacccggcaa caacgcaacc catgttcagt acgctgttcg agggtgataa
     9061 acagcgcgtc gagagtgaaa cgctccacct gggtgaagca gggccagccc agcatttccg
     9121 ccagcaaaaa tggcgtttgc ccattctgcc cttcgctgct ttggcagccg gtaattatca
     9181 gatccagcgg attctgatgc tgccattcgg caatatggcg agcgacaaat tccggcgcaa
     9241 agcgcagatc tgccgccgtc tccagcaata cagcttcctc aaacccaaga gccatgagat
     9301 agcgcagcca gtgcaatgcc cgttcatccc ccatgctcaa cgcggttaaa gacatcggcg
     9361 taccgttttt tctctgcgcc aacagcagcg cggcagcggc ctgttcatca gcaccgagta
     9421 aacttcgcag tagcgaaata tccggtccgc ttttaccctg agccgccgcc tgccactctt
     9481 tttccgccag cattccggca tccggttcgg ctttaaacgc taacagaatg ttcattcgca
     9541 cctcctacat ttcccgccgc caccagcggg agggctttag tttctggtgc ccatagccag
     9601 gtcacaacaa aatcaaccaa caacactgtc gccaggagca ataaggtgac ttgcattccc
     9661 cactgcgcca gcacccacgg cagcaggcca gtacttaccg ccgcgccaag tcgactcatg
     9721 gcggtggcaa aaccgacgcc cagcgagcga atgtcagtag gaaaactttc cgcaggcaaa
     9781 atgcccacca gattactgac tgccgaaatg gtggtgctga agagaacaaa aagcagcagc
     9841 gttaatgaac tgccggaagg caaacaggcc atcactacca gcgttgccgc cagcagcaaa
     9901 aaacttccca gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa tcccagcagc
     9961 gcgcccacaa ttaacaacgc attaagcatc aggctggcag tcagcgcatc ttccagacca
    10021 atagtctggg cgatagttgg cagccaggta taaatcacaa accatgggat tacgaggcag
    10081 acaaagaaga cgctgttaaa cgccgtgcgc cgccagtaac gcgaagagaa caaggttttg
    10141 atgtgtttat gggtcgccgt taccacttca tcgcccagta aaacatgggg accaaaatag
    10201 cgatgcacga tagcgtgagc ttctgcaaaa cgcccctggc gcagtagcca gcgtggtgat
    10261 tctggcgttc cccagcgtaa taacgtaatc aacaacgcgg gcagagctgc cgatgccagt
    10321 agccagcgcc aggcctccgg gttttcggaa ataaagtgat gtccggcaat acttgccagc
    10381 acatagccta cggtccacac cacgctgaat gcgcccagca aaataccgcg atggcggcgc
    10441 ggggaaaatt cagccagcaa ggttaatgcc attaggatcg aactgctttt tcagcccttc
    10501 cagcaacgcc cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat
    10561 accgtggtgg tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt
    10621 gagcggattg tggtacttgt cgatttcctc ttccggctta cagtcaacga cgttgtaatc
    10681 gtagacgaag tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat
    10741 ggtgatgtcg tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat
    10801 ttcgtggatg cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt
    10861 gaggatctgc acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca
    10921 ggtttcgatc agcttgctgt ccacgcgctg gcattgcggg tagcgggcaa cgatttccgc
    10981 aatcccttcg cccgtcgcct tcgcaatgcg agggttacct tcagccataa agatcagcac
    11041 gcattttccg tctgcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg
    11101 agcgatcgac ggacgatacc cttccaccat gacttcacgc aggatgttga agtcggtttt
    11161 catgtcttcc aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt
    11221 cactgttact tcagtgatat agcaaagtgc accttcgtta ccgatgatga tgtggcgaat
    11281 gtccgggcca gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa
    11341 tactgcttcc agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc
    11401 gatactgcgg gttgctacca agccgcccat ctgcgccagc ggctttgact gcggagaatg
    11461 ccccgtggtg taaccttttt cacgcaacgc attttccagc acctccagcg gaacgccaca
    11521 ttgcgccgtc gcctgcatat tctcaatatc aatattaatg atttgattca tggcggagcc
    11581 gtcaagcacc accgagtttt ccacaacggt ttccagccca ccttcagtgg cggaagcgcc
    11641 ggtacgcggc acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggatacttg
    11701 ctctgtggaa ccgagtttta cgaccgctgc cggaatcggc aaagtataaa tgccatgaat
    11761 atccggaaat ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat
    11821 tacgcgatct gcaccaacaa tttcctttag ttggtcgaca atcgctgcgc gagataaaga
    11881 catagtaatt ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac
    11941 caccgttatg gattagcgca ctaaataacc accattgacc actaataaat gcccattgac
    12001 ataatttgaa gccggacttg cgaggaatac agctgcgccc attaaatcct gagtatcgcc
    12061 ccaacggttt gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt
    12121 cgccagcgta atatctgttg cataatagcc aggagcaata ccatttacct gaatattata
    12181 ttgacctagt tcatcacaat aagctttggt gaatccggca agagcatgtt tagtggcaga
    12241 atatgcaggt gaccattgtc cacctaagta agagaacaat gaacagatat taataatttt
    12301 gccgcttttc tgcgggatca taatttttgc agcttcatag cttaactcga atgcggcggt
    12361 caggttcaca tcaatcatcg gatcccagtc ggcacgaccg aagtccagca ccttattcag
    12421 cttacaaata ccggcattgt ttaccagaat atcaactgta ccgaaacgct cacagcaagc
    12481 agcgataatc ttctgcggcg cgccttctgc ggtgatatcc acctgcatga agtccacctc
    12541 aacaccctgt ttttcaaaca tttcctttgt ttcgccgtta tctttcaatc atttcctttg
    12601 tttcgccgtt atctttgacg aaactaggaa taaagatatt tgcgccagct ttggccaacg
    12661 ccatggcaaa tgcctggcct aaaccgctat tcccaccggt aacaattgcg gttttacctt
    12721 tcagtgagaa aaaatccatt gagaacgcat tgagagattc gattgacata gttagctcca
    12781 atttccgtaa ggcaaaaaaa agaaaggtaa cttcccccgc gagagggaag ttacctttct
    12841 cattatctct ggtaactgga gataattttt agcataacgg cataactgct aatgtgatcg
    12901 taatcacagt gtgatattcg tattgtcaaa tagttttaaa aagcagaagc gtgattactc
    12961 tcacacaaca cctctgcttt ccatgttatt tcttattgca attactagag acctgagaaa
    13021 agtaatccac ctgaaagggc ggcttacttt tctctttttt ttgtctatta caaatgggaa
    13081 tacgatatgc aacacaactc atatcgccgt tggataaccc tcgcgataat tagttttagc
    13141 ggcggcgtta gttttgacct ggcttattta cgttatattt atcaaattcc catggcgaaa
    13201 tttatgggat tcagcaatac cgagataggt ttaataatga gtacctttgg tattgcggct
    13261 attattcttt atgcccccag cggcgttatt gccgataaat tttcacaccg caaaatgatt
    13321 acttccgcga tgatcattac cggattactg ggtctgttaa tggcaacgta tccaccgctg
    13381 tgggtaatgc tctgtattca ggtcgccttt gcgataacga cgattttaat gctgtggtcg
    13441 gtgtcgatta aagccgcctc gttgctgggc gatcatagtg agcaagggaa aattatgggc
    13501 tggatggaag ggctgcgcgg cgtcggtgta atgtcgctgg cggtgtttac catgtgggtc
    13561 ttttctcgct ttgcaccgga tgacagcgcc agcctgaaaa cagtcattat catctacagt
    13621 gtggtttaca tcttgttggg gattctgtgc tggttttttg ttagcgataa caacaacctg
    13681 cgcagtgcca ataacgaaga aaaacagtca ttccagctta gcgacatcct tgccgttttg
    13741 cgtatcagca ccacctggta ttgcagcatg gtgatttttg gtgtcttcac aatctacgcc
    13801 attctgagtt actccaccaa ctatctgacc gaaatgtacg gcatgtcgct ggtggcagcg
    13861 agctacatgg ggattgtgat caacaaaatc ttccgcgcgc tgtgcggccc acttggaggc
    13921 attatcacca cctacagtaa agtgaaatcc cctacccgcg tgattcaaat cctttccatc
    13981 atcggtttgc tggcgttgac agccctgctc gtcacgaact ctaaccccca atcggtcgcg
    14041 atggggattg gcctgatttt actgctggga ttcacctgtt acgcctcacg cgggctgtac
    14101 tgggcctgcc ccggcgaagc gagaacaccg tcttacatta tgggcaccac ggtaggtatt
    14161 tgttcggtga ttggattcct gccggatgtc ttcgtttacc cgattatcgg ccactggcaa
    14221 gacaccctgc ccgctgcaga agcctaccgc aatatgtggc tgatgggcat ggcggcgctc
    14281 ggcatggtga ttgtctttac ctttttgctg ttccaaaaaa ttcgtactgc tgatagcgcc
    14341 cccgcaatgg ctagcagcaa gtaagccagg aaaattacag gagaaaaatc atgtcgaaaa
    14401 aatacatcat agggattgat ggcggaagtc agagcacaaa agtggtgatg tacgatctgg
    14461 aaggtaatgt ggtttgcgaa ggtaaaggct tattacagcc gatgcacacg cctgatgccg
    14521 atactgcaga acatcctgac gacgatttat gggcatcatt atgttttgca ggtcacgatt
    14581 tgatgagcca gtttgccggg aataaagaag atattgttgg catcggcctg ggctccatcc
    14641 gttgctgccg tgcgttattg aaagccgacg gcacgccagc tgcgccgttg attagctggc
    14701 aggatgcacg cgttacacgc ccttacgaac atacgaatcc tgacgtggcg tatgtaacct
    14761 ctttttcggg ttatctgacg catcgcttaa ccggcgaatt taaagataat atcgccaact
    14821 attttggtca gtggccggtg gattataaga cctgggcatg gagcgaagat gccgcagtca
    14881 tggagaagtt taatatcccc cgtcatatgc tgtttgatgt gcaaatgcca ggcactgttc
    14941 ttgggcatat cacacctcaa gccgcgctgg cgacgcattt cccggcagga ctgccggttg
    15001 tttgtaccac cagtgataaa ccggtagaag ctctgggagc cggattactg gatgatgaaa
    15061 ctgcggtaat ttctcttggc acttacatcg cattgatgat gaacggcaaa gccttgccga
    15121 aagatccggt ggcgtactgg ccgattatgt cttctattcc gcaaacattg ctgtatgaag
    15181 gttacggtat tcgcaaaggc atgtggacgg tgagctggct gcgcgacatg ttaggcgagt
    15241 cgttaattca ggatgccagg gcgcaggatc tttcaccgga agatttactc aacaagaaag
    15301 cctctagtgt gccacctggc tgtaatgggc tgatgacggt gctggactgg ctgaccaatc
    15361 cgtgggaacc gtacaaacgc gggattatga tcggctttga ttccagcatg gattacgcat
    15421 ggatatatcg ttcgatattg gaaagcgtgg cgctgacgct gaagaacaat tacgacaata
    15481 tgtgtaatga aatgaatcac tttgcgaaac atgtaatcat tactggcggc ggttcgaaca
    15541 gcgatctgtt tatgcagatt tttgccgacg tgttcaacct tccggcacgc cgtaacgcca
    15601 ttaacggttg tgcaagcctg ggggcagcga ttaatacagg ggtaggtctg gggctatacc
    15661 cggattacgc aacggctgtc gataaaatgg ttcgcgtgaa agatatcttt ataccgattg
    15721 agagcaatgc caaacgctac gacgcgatga ataaaggcat tttcaaagac ctaaccaaac
    15781 atactgatgt gatcctgaaa aaatcgtatg aagtgatgca tggggaattg gggaattggg
    15841 gaatgcggat tcgatccaga gctggtcgaa tgcgtgaata aggtaggccg ggcggaaaac
    15901 tgcccggcct gaacatacct gaatggttat ccccgctggc gcggggaaca taagttcgac
    15961 aacttaaaaa atgataacaa gtccggttta tccccgctga tgcagggaac ataataaaaa
    16021 ctttaactag ttcggtttat ccctgttttt acaaggaa
//
LOCUS       DAZOZY010000084        16001 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZOZY010000084 DAZOZY010000000
VERSION     DAZOZY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.084"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            85..1515
                     /locus_tag="ACHURQ_002329"
     CDS             85..1515
                     /locus_tag="ACHURQ_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0824085.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(1556..2488)
                     /locus_tag="ACHURQ_002330"
     CDS             complement(1556..2488)
                     /locus_tag="ACHURQ_002330"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR0824086.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2852..5638
                     /gene="polA"
                     /locus_tag="ACHURQ_002331"
     CDS             2852..5638
                     /gene="polA"
                     /locus_tag="ACHURQ_002331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR0824087.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(6020..6652)
                     /gene="yihA"
                     /locus_tag="ACHURQ_002332"
     CDS             complement(6020..6652)
                     /gene="yihA"
                     /locus_tag="ACHURQ_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR0824088.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6949..7115
                     /locus_tag="ACHURQ_002333"
                     /pseudo
     CDS             6949..7115
                     /locus_tag="ACHURQ_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7234..7743
                     /gene="yihI"
                     /locus_tag="ACHURQ_002334"
     CDS             7234..7743
                     /gene="yihI"
                     /locus_tag="ACHURQ_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR0824089.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7932..9305
                     /gene="hemN"
                     /locus_tag="ACHURQ_002335"
     CDS             7932..9305
                     /gene="hemN"
                     /locus_tag="ACHURQ_002335"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR0824090.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9495..9605)
                     /locus_tag="ACHURQ_002336"
     CDS             complement(9495..9605)
                     /locus_tag="ACHURQ_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR0824091.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9717..11126)
                     /gene="glnG"
                     /locus_tag="ACHURQ_002337"
     CDS             complement(9717..11126)
                     /gene="glnG"
                     /locus_tag="ACHURQ_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR0824092.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(11138..12187)
                     /gene="glnL"
                     /locus_tag="ACHURQ_002338"
     CDS             complement(11138..12187)
                     /gene="glnL"
                     /locus_tag="ACHURQ_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR0824093.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(12361..13770)
                     /gene="glnA"
                     /locus_tag="ACHURQ_002339"
     CDS             complement(12361..13770)
                     /gene="glnA"
                     /locus_tag="ACHURQ_002339"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR0824094.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            14143..15966
                     /gene="typA"
                     /locus_tag="ACHURQ_002340"
     CDS             14143..15966
                     /gene="typA"
                     /locus_tag="ACHURQ_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR0824095.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         4189 a         3937 c         4093 g         3782 t
ORIGIN      
        1 ttctgataat gcgtcctgcc gctggacatt atttcagcat aagcggatat ttaaagtttt
       61 gctgttttat cagggaatat ttatatgata cgtaagtcag ctacaggtgt tattgttgcg
      121 ttagccgtaa tctggggtgg tggcacatgg tacacaggta cgcaaattca gcctggtgtc
      181 gaaaagttta ttaaagattt taacgatgct aaaaagaaag gtgaacatgc ctacgatatg
      241 acgttaagtt ataaaaattt tgacaaaggt ttttttaatt ctcgttttca aatgcaaatg
      301 acattcgata acggtgcacc cgatctcaat atcaagccag gccagaaagt tgtatttgat
      361 gtggatgttg agcacggtcc gttgcccatc acaatgttaa tgcatggtaa tgtcatccca
      421 gcactggcag cggcaaaagt gaacttagtg aataatgaac tgacacaacc gctatttatc
      481 gccgcgaaaa ataaatcgcc cgtggaagcg acattgcgat tcgcgtttgg tggctcattc
      541 tctacgacat tagatgttgc ccctgcagag tatggaaagt tttcttttgg tgagggccag
      601 tttactttta atggtgatgg tagttcattg tctaacctgg atattgaagg caaagtcgaa
      661 gatattgttc tgcaattatc accaatgaac aaagtaacgg caaaaagttt taccattgat
      721 tctctggcgc gattagaaga aaagaaattt ccggttggtg aaagcgagtc gaaatttaat
      781 cagattaaca ttatcaatca cggggaagac gttgcccaaa tcgatgcttt cgttgcaaaa
      841 accatgctgg atcgcgttaa agacaaagat tatatcaatg tcaatctgac ctacgaactt
      901 gataagttaa caaaagggaa tcagcaactc ggtagtggtg agtggtcatt gattgctgaa
      961 tctattgatc cctcagcagt gcgccaattt atcatccagt ataacattgc tatgcagaag
     1021 cagcttgctg tacaccctga gttagcaaac gatgaagttg ctctgcaaga agtgaatgct
     1081 gcattgttca aagagtattt accgttatta caaaaaagtg agccgaccat taaacaaccg
     1141 gtaagatgga agaacgcact cggcgaacta aatgccaatc tggatatcag tattgccgac
     1201 ccagccaaat cttcatcatc cacaaacaaa gatatcaaat tgctcaattt tgatgtgaag
     1261 ttaccgctta atgtcgtcac agaaaccgca aaacagctta atttatctga aggaatggat
     1321 gcggaaaaag cgcaaaagca ggctgataaa caaatcagcg ggatgatgac attgggtcag
     1381 atgtttcagt taatcacgat tgacaacaat accgcctcgc tgcagctgcg ttatacaccg
     1441 ggtaaagttg tgtttaacgg acaggagatg agcgaagaag aatttatgtc tcgtgccgga
     1501 cgttttgttc attaaaatac tcactcgcct ctgctcattc agaggcgagt aaaacttact
     1561 tattctgacg ctgtgatgac atgagagacg tcagaagccg gtctttttcc tgccacaatg
     1621 agttcagcca ttgctgaaaa tggcgcttaa agcttttgtc attgatgtag tcgccatgta
     1681 actcatcggc aataggctgt aaatccacat gtacgacaat ccgcgtcagt ttaccgctta
     1741 acatatcgaa gaacggctga cggttattgt ccggataaca cagcgtaaca ttaagcagtt
     1801 tatcgaattg tttacccagt acatttagcg ccatcgcaat gcctgccgcc tttggtggca
     1861 acaagttttg aaaagtggag tgggtttgct gatgtttttc ttgcgtgaaa cgggagcctt
     1921 caacgaaatt gacaatggtg gtgggatgca ggcgaaactt ttcacaagaa cgccgagtgg
     1981 tttcaacatc tttaccacgt cgctccggat gacgtaataa ataagcgcgg gaataacgct
     2041 tcataaacgg catatccagc gcccagcacg ccaggccaag aaatggcacc caggccagct
     2101 gttgcttgag gaaatattta ttcatcggaa tgtgcttacg aaacagcacg cacagtacga
     2161 caatatctgc ccaactacgg tggttacaaa taagcagata ccagttcttc ttacttaacc
     2221 cttccagccc gtgaacttcc cattgcaggt gtgggttcag atgcagtaat accgccagac
     2281 cttcacacca gcaatacatc ataaaatcac aaaaacgcga aacctttcgc caaatgactg
     2341 gcacaggtag caatagtttt acaatcccgg caatgatgat cgggacggaa caaaaaatag
     2401 tgaccaaaat ggtcaatacg atactcagca aaagggttat cgcagcgagt attctcgtca
     2461 taatgaattt attcaaaagg ttagccatag acagtgcgct aaagaagcgc aaggcgcaga
     2521 ttttagcaga aaacgaccca aataacggat gatccttaag gagaaaaata atccatatct
     2581 atccacatta gaaaaaatcc cattatctca attattaggg atggctttat ttttaactgc
     2641 atgaaaaaca aagacaaaca tcatgctgta aaaagcatga taataaatta aaagcgatgt
     2701 aaataattta tgcacaaagt tatccacatg acgatttgcg agcgatccag aagatctaca
     2761 aaagattttc acgaaaagcg gtgaaaaact catgttttca tcctgtctgt ggcatccttt
     2821 acccataatc tgataaacag gcacggacat tatggttcag atcccccaaa atccacttat
     2881 ccttgtagat ggttcatctt atctttatcg cgcatatcac gcgtttcccc cgctgactaa
     2941 cagcgcaggc gagccgaccg gtgcgatgta tggtgtcctc aacatgctgc gcagtctgat
     3001 catgcaatat aaaccgacgc atgcagcggt ggtctttgac gccaagggaa aaacctttcg
     3061 tgatgaactg tttgaacatt acaaatcaca tcgcccgcca atgccggacg atctgcgagc
     3121 acaaatcgaa cccctgcacg cgatggttaa agcgatggga ctgccgctgc tggcggtttc
     3181 tggcgtagaa gcggacgacg ttatcggtac tctggcgcgc gaagccgaaa aagccgggcg
     3241 tccggtgctg atcagcactg gcgataaaga tatggcgcag ctggtgacgc caaatattac
     3301 gcttatcaac accatgacga ataccatcct cggaccggaa gaggtggtga ataagtacgg
     3361 cgtgccgcca gaactgatca tcgatttcct ggcgctgatg ggtgactcct ccgataacat
     3421 tcctggcgta ccgggcgtcg gtgaaaaaac cgcgcaggca ttgctgcaag gtcttggcgg
     3481 gctggatacg ctgtatgccg agccagaaaa aattgctggg ttgagcttcc gtggcgcgaa
     3541 aacaatggca gcgaagctcg agcaaaacaa agaagttgct tatctctcat accagctggc
     3601 gacgattaaa accgacgttg aactggagct gacctgtgaa caactggaag tgcagcaacc
     3661 ggcagcggaa gagttgttgg ggctgttcaa aaagtatgag ttcaaacgct ggactgctga
     3721 tgtcgaagcg ggcaaatggt tacaggccaa aggggcaaaa ccagccgcga agccgcagga
     3781 aaccagtgtt gcagacgaag cgccagaagt gacggcaacg gtgatttctt atgacaacta
     3841 cgtcaccatc cttgatgaag aaacactgaa agcgtggatt gcgaagctgg aaaaagcgcc
     3901 ggtatttgca tttgataccg aaaccgacag ccttgataac atctctgcta acctggtcgg
     3961 gctttctttt gctatcgagc caggcgtagc ggcatatatt ccggttgctc atgattatct
     4021 tgatgcgccc gatcaaatct ctcgcgagcg tgcactcgag ttgctaaaac cgctgctgga
     4081 agatgaaaag gcgctgaagg tcgggcaaaa cctgaaatac gatcgcggta ttctggcgaa
     4141 ttacggcatt gagctgcgtg ggattgcgtt tgataccatg ctggagtcct acattctcaa
     4201 tagcgttgcc gggcgtcacg atatggacag cctcgcggaa cgttggttga agcacaaaac
     4261 tatcactttt gaagagattg cgggtaaagg caaaaatcaa ctgaccttta accagattgc
     4321 cctcgaagag gccggacgtt acgccgccga agatgcggat gtcaccttgc agttgcatct
     4381 gaaaatgtgg ccagatctgc aaaaacacaa agggccgttg aacgtcttcg agaatatcga
     4441 aatgccgctg gtgccggtgc tttcacgcat tgaacgtaac ggtgtgaaga tcgatccgaa
     4501 agtgctgcac aatcattctg aagagctcac ccttcgtctg gctgagctgg aaaagaaagc
     4561 gcatgaaatt gcaggtgagg aatttaacct ttcttccacc aagcagttac aaaccattct
     4621 gtttgaaaaa cagggcatta aaccgctgaa gaaaacgccg ggtggcgcgc cgtcaacgtc
     4681 ggaagaggta ctggaagaac tggcgctgga ctatccgttg ccaaaagtga ttctggagta
     4741 tcgtggtctg gcgaagctga aatcgaccta caccgacaag ctgccgctga tgatcaaccc
     4801 gaaaaccggg cgtgtgcata cctcttatca ccaggcagta actgcaacgg gacgtttatc
     4861 gtcaaccgat cctaacctgc aaaacattcc ggtgcgtaac gaagaaggtc gtcgtatccg
     4921 ccaggcgttt attgcgccag aggattatgt gattgtctca gcggactact cgcagattga
     4981 actgcgcatt atggcgcatc tttcgcgtga caaaggcttg ctgaccgcat tcgcggaagg
     5041 aaaagatatc caccgggcaa cggcggcaga agtgtttggt ttgccactgg aaaccgtcac
     5101 cagcgagcaa cgccgtagcg cgaaagcgat caactttggt ctgatttatg gcatgagtgc
     5161 tttcggtctg gcgcggcaat tgaacattcc acgtaaagaa gcgcagaagt acatggacct
     5221 ttacttcgaa cgctaccctg gcgtgctgga gtatatggaa cgcacccgtg ctcaggcgaa
     5281 agagcagggc tacgttgaaa cgctggacgg acgccgtctg tatctgccgg atatcaaatc
     5341 cagcaatggt gctcgtcgtg cagcggctga acgtgcagcc attaacgcgc caatgcaggg
     5401 aaccgccgcc gacattatca aacgggcgat gattgccgtt gatgcgtggt tacaggctga
     5461 gcaaccgcgt gtacgtatga tcatgcaggt acacgatgaa ctggtatttg aagttcataa
     5521 agatgatgtt gatgccgtcg cgaagcagat tcatcaactg atggaaaact gtacccgtct
     5581 ggatgtgccg ttgctggtgg aagtggggag tggcgaaaac tgggatcagg cgcactaaga
     5641 ttcgcctgaa catgcccttt tttcgtaagt aagcaacata agctgtcacg ttttgtgatg
     5701 gctattagaa attcctatgc aacaactgaa aaaaaattac aaaaagtgct ttctgaactg
     5761 aacaaaaaag agtaaagtta gtcgcgtagg gtacagaggt aagatgttct atctttcaga
     5821 ccttttactt cacgtaatcg gatttggctg aatattttag ccgccccagt cagtaatgac
     5881 tggggcgttt tttattgggc gaaagaaaag atccgtaatg cctgatgcgc tatgtttatc
     5941 aggccaacgg tagaattgta atctattgaa tttacgggcc ggatacgcca catccggcac
     6001 aagcattaag gcaagaaaat tattcgccgt cctgcgtttc ttctacaggc tgcatctcgc
     6061 taaaccaggt atccagtttc tgccgcagct tgtccacgcc ttgtttcttc aacgaagaaa
     6121 acgtttcaac ctgcacatca ccgttaaacg ccagtacagc ttcacgcacc atattcaatt
     6181 gcgctttacg tgcgccgctt gccagtttgt ccgctttggt cagcagcacc agaacggcga
     6241 tattgctgtc taccgcccac tcaatcatct gctgatccaa atctttcagc ggatggcgaa
     6301 tatccattag caccaccaga ccttgcaggc tctgacgttt ttcgaggtat tcgccgagcg
     6361 cacgctgcca tttgcgcttc atctcttccg ggacttccgc ataaccgtac ccaggcaagt
     6421 caaccagacg cttgccgtca gccacttcaa acaggttgat aagctgggtg cgccctgggg
     6481 tttttgaggt acgagccagg cttttctggt tagtcagcgt gttcagcgcg ctggatttac
     6541 ctgcgttgga acggcctgca aaagccactt caattccggt atcggaaggt aggtggcgaa
     6601 tatcaggcgc actcatcaca aaatgcgtct gttgataatt caaattagtc aaagcggtcg
     6661 tctccgtcag tcaaagcttt gtggcgatta tacctgaacc ataataaaag gcggattttt
     6721 cggcgtgagc gttgtaagta aaagccatac gctttgtgag acattgccga tagtctttat
     6781 gcgaaatagc agagaaaatt ctgcgatgca tgtcaaataa gctatataaa tcagtgaatt
     6841 gcttttatat aaaactctga aaatgtgcaa atactgatgg cggttgattg tttgtttaaa
     6901 gcaaaggcgt aaagtagcac ccatagagcg aggacgctaa caggaacaat gactcaggat
     6961 gagggtcagg agcgccagga ggcgaagaca gaggattgtc aggaagacaa acgtccggag
     7021 acgtaattaa acggaaatgg aatcaacacg gattgttccg gctaaaggaa aaacagggtg
     7081 tgttggcggc ctgcaaggat tgtaagaccc gttaagggtt atgagtcagg aaaaaaggcg
     7141 acagagtaat ctgtcgcctt ttttctttgc ttgctttctg ttagattccg ccgcaaatct
     7201 atactgaata aaacggctaa aagacgaacc attatgaaac catcatcttc aaactcacgc
     7261 agcaaaggtc atgcaaaagc gcgtcgaaaa acacgcgagg agctggatca ggaagctcgt
     7321 gatcgcaagc gtcagaaaaa acgtcgtggt catgcgccgg gcagccgcgc agcgggcgga
     7381 aacaccacgt caggcagcaa aggccagaac gcaccaaaag atccacgtat tggcagtaaa
     7441 acccctattc cattgggcgt ggctgaaaaa gtcaccaaac agcacaaacc gaagagtgag
     7501 aaacctatgc tttcaccgca ggcggagttg gaattactgg aaacggatga gcgtctggat
     7561 gcgctgctgg aacgtctgga agcaggcgaa accctgagcg ccgaagagca atcctgggtg
     7621 gatgctaaac tggatcgtat tgatgagttg atgcagaaac tcggcctctc ttatgacgat
     7681 gacgaagaag aggaagaaga cgagaagcaa gaagacatga tgcgtctgct gcggggtaac
     7741 taacggattg ccaccgtggg ccttcccgtt ctgctgataa cccttccggt tatatgttat
     7801 ctggtgtggt tattcgttaa actacagcgg ttgtcgcggc gacaaaagtg gctgcgcaac
     7861 cggctgatga cccgaaacgg gcatccgccg gtacgccgta gccgccagag acgccatcgg
     7921 aaggagtgag catgtctgta cagcaaatcg actgggatct ggccctgatc cagaaatata
     7981 actattccgg gccacgatac acctcgtacc cgaccgcgct ggagttttca gaagacttcg
     8041 gcgaacaggc gtttttacaa gccgtggcgc gctatcctga gcgtccatta tctctctacg
     8101 tacatatccc gttctgccat aagctttgtt acttctgcgg ttgcaataag attgttactc
     8161 gccagcagca caaggccgat cagtatctgg acgcgctgga gcaagaaatc gtccatcgtg
     8221 caccgctgtt cgccgggcgt cacgtcagcc aattgcactg gggcggcgga acgccgacgt
     8281 atctgaataa agcgcaaatc agccgcctga tgaagctgct gcgcgaaaac ttccagttca
     8341 atgccgatgc ggagatttcg atcgaagtcg atccgcggga aatcgaactg gatgtactcg
     8401 atcatttacg cgccgaaggc tttaatcgcc tgagcatggg cgtgcaggac ttcaacaaag
     8461 aagtgcaacg tctggttaac cgcgagcagg atgaagagtt catctttgca ctgcttaacc
     8521 atgcgcgtga gattggtttt acctccacca acatcgacct gatttacggc ctgccgaaac
     8581 agacgccgga gagtttcgcc tttaccctga aacgtgtggc ggagctgaac cccgatcgtc
     8641 tgagtgtctt taactacgcg catctgccga ccatttttgc tgctcagcgc aaaatcaaag
     8701 atgctgacct gccgagtccg cagcaaaaac tcgatatcct gcaggaaacc atcgccttcc
     8761 tgacgcaatc gggctatcag tttatcggta tggatcactt tgcccgtccg gatgacgagc
     8821 tggcggtggc ccagcgtgaa ggcgtgctgc atcgtaactt ccagggctac accactcagg
     8881 gcgataccga tctgctgggg atgggcgttt ccgccatcag catgattggc gactgctacg
     8941 cgcagaacca gaaagagttg aagcagtact atcagcaagt ggatgaacaa ggcaatgcgc
     9001 tgtggcgtgg tattgcgcta acgcgtgatg actgtattcg ccgcgatgtg attaagtcgc
     9061 tcatctgcaa cttccgtctg gattacgccc ctattgagaa acagtgggat ttgcacttcg
     9121 ctgattactt tgcggaagat ctcaagctgc tcgccccgtt agcaaaagat gggctggtgg
     9181 atgtggatga gaagggaata caggtgacgg cgaaaggtcg cttgctgatc cgcaacattt
     9241 gcatgtgctt tgatacctat ctgcgccaga aagcgcggat gcagcagttc tcacgggtga
     9301 tttaaatagt gaatggcgct tcgtttacaa agtagaaaac gaaagcgcca tcaatgtgct
     9361 gagaacaaga ttgcctgatg cgctacgctt atcaggccta caaaacctgt tgaaaatatt
     9421 gcttttgtag gccggataag gcgttcacgc cgcatccggc atgaacaaag agcacttcat
     9481 cagcaatctg aaactcaact aaacagccca atcattgcgg cacacagcac ggcagcaacg
     9541 gcagtttgcg gtgtgtggct gtcaactgcg ccactcgata ccagattaat tattgattcc
     9601 agcataatga ctctccccgt tttccgggca agatcatact gaacttatcg gaacagtaaa
     9661 gcgtaaaata ccagcaattt gcgctcaata atcaatcttt acacacaagc tgtgaatcac
     9721 tccatcccca gctcttttaa cttacgcgtc agggtgttgc ggccccagcc gagtagccgc
     9781 gccgcttcct gtttatgccc ctgcgtatgt cgcaacgcgg tcgtcagtaa cgtccgctcc
     9841 agctctggct gcgcttcgga aagcagattt tgatgaccgg aacgcagcgc tctgtctgcc
     9901 cactgcgcaa gaagcgttgc ccagctgtcc ggttgcattt gcgaagtact ctccgcaacc
     9961 gttgattcaa acagttcgcc gggcaaatcc tgaatcaaca cttcctgccc ggcggccatc
    10021 accgttagcc agcggcaggt gttttccagc tggcgcacgt tgcctggcca cgccagacgc
    10081 gtcagagcag cttcggtttc cggatgcagt aacttcgctt ctacgcccag ttcgcgcgcg
    10141 gcaacctgta aaaaatggcg cgccagacgg ggaatatctt cccgacgttc gcgcagcggc
    10201 ggcagatgaa cgcggataac gttcaggcgg tggaacagat cctcacggaa cttaccttcc
    10261 tgcactcgtt gttcgagatt ctggtgagtg gcagcgataa tacgcacatc cactttcacc
    10321 ggcgcatagc cgccaacgcg gtaaaactga ccgtctgcca gcacgcgcag caaacgcgtc
    10381 tgcacatcca gcggcatatc accaatttcg tcgaggaata atgtaccgcc atcggcctgt
    10441 tcaaaacgcc cctgacgaat ggtattcgcg ccagtaaacg cgcctttctc gtggccaaac
    10501 agttctgatt cgatcaaatc ttttgggata gctgccatat tcagcgcgat aaacggcgct
    10561 ttggcgcgcg gactgtggcg atgcagggca tgagcgacca gttctttacc ggtgccggat
    10621 tcgccgttaa tcagcacgct aatagaagaa cgcgaaagcc gaccgataat acggaacacg
    10681 tcctgcatgg ctggcgcttc gccgatgata tcggtcgttg ggccgttaag ctgaatatta
    10741 cgcggctgct gctgttcctg gtaatgactg atagcgcgct caaccagcgc cactgcttcg
    10801 tcgatatcaa acggtttggg cagataatca aacgcccctt gttgataggc gctgacggca
    10861 gcatccagat cggaatgtgc ggtcataatg atgaccggaa gcattggatg gcgctgttta
    10921 atctgcttga gcagcgccag cccgtccatt cccggcatac ggatatctga aagcagcaca
    10981 tccggcgttt tgctcgccag cgcctccagt acttccgcgc cgttctcaaa tgtcgtacag
    11041 gttaaacccg ctccagcgag cgcacgttca agcacccaac ggatggaact atcgtcatcg
    11101 actacccaga ctatccctcg ttgcataaac gtcaccttta tttcctgata ggcaggtaaa
    11161 ccgagaactc ggtatgacct ggccaactgg taaattcaat tttgcctgaa tgctgatcaa
    11221 tcaaattacg ggcgatggat aagccaagcc cggtgccacc ttcgcggccg ctgaccatcg
    11281 ggtaaaacag cgtatcctgc aaatgaggcg gaatgcccgg cccgttatct tccacatcaa
    11341 tccgcgccgc cagccggtag cgctcgccgt gtaaggtcag ttgaaacgcg gtgcgggtac
    11401 gcagaatgat ttcaccgcct tccggcccca gcgcctgtag cgcattgcgc acaatattca
    11461 gcaagacctg ttcaatttga tccgggtcgt gcgccagttc cggcaggctg gggtcgtaat
    11521 cacgaatcaa ccgcacgttg tccggcagtt ccatcgacac cagcgtcacc acgcgttcag
    11581 ctactttgtg aatactttcg gtaacgcgcg taccgggcag ctgcggcccc aacagacggt
    11641 cgaccagatt tcgcagccgg tccgcctgtt cgataatcac tttggtatat tcgagtagtg
    11701 atgggtcagg taacgctttg ctgagcagct gcgccgcgcc acgtaaacct ccaagcggat
    11761 ttttaatctc atgtgccagg ccgcgcacta aatcacgggc agcaacctgc tgggcgtgct
    11821 gtagctgttc ctgacttaag cggcgctggt tatccatcgg agccatctcc agcaggatca
    11881 tgccgtccgg catacgctgg gccgtcacag aaaggatatg cgagcgcccg tcgatgacca
    11941 gcgtcacttc gttatcggta aaaccttgcc ccgcctccag actttcttgc atcagctcga
    12001 tatttaatga gaagtagctc aacagttccg gtaacggtgt accaaacaat ttgcgggagc
    12061 tttgggcgag cagttgttgc gcggcggggt tggcgtaatg gatcgccaga ttgtcatcaa
    12121 ttaacaaaat actgttaatc agcgagttga ggatctgccc agcatcgggc tgcgtgcctg
    12181 ttgccataaa gcagtctcct gaacaggttg caccatttta gtgcattata gctttttacg
    12241 gataaaaagc gcgaagcatc agagaattga cggagaaaaa agcccatgca gagatgggct
    12301 gaaagtttcc acggcaacta aatcccggcg ttgttgcgcc gggtagtaca acttcaacta
    12361 ttagacgctg tagtacagct caaactctac cggatgcgga gtcatacgca cgcggtcatc
    12421 ttcttcgcga cgtagagcga tgtacgcatc aattgcttcg tcagtgaaca cgccaccggc
    12481 tttcaggaac tcgcggtcca gatccagttc gttcagtgct tcttccagag agcctgcaac
    12541 ctgtgggatc tctttcgctt cttctggcgg caggtcatac aggtttttgt ccatggcttc
    12601 gcccggatgg atcttgttct tgataccatc aagaccggcc atcagcaggg cagcaaagca
    12661 caggtacggg ttagctgccg gatccgggaa acgtacttcg atacgacgtg ctttcggaga
    12721 agaaaccacc ggaatacgga tagacgcaga acggttacgc gcagagtaag ccagcattac
    12781 cggtgcttca tagcccggga ccagacgctt ataagagttg gtggtcgggt ttgccagggc
    12841 gttaatcgct ttagcgtgtt tgattacgcc gccaatgtag tacagcgcct gctcagacag
    12901 acctgcgtat ttgtcgcctg cgaacaggtt aacgccgttt ttagacagag acatgtggca
    12961 gtgcataccg gagccgttat caccgaacat cggttttggc ataaaggtcg cggttttacc
    13021 gaagcggtgc gctacgttgt gcacaacata tttgtagatc tgaatttcgt cagctttttt
    13081 ggtcatggta ttgaagcggg tagccacttc gttctgacca gcagtcgcta cttcgtggtg
    13141 atgggcttca accaccagac ccatctgttc catcaccaga cacatttcag aacgaatatc
    13201 ctgagccgag tctaccggtg gaaccgggaa gtaaccgcct ttcactgccg gacggtgacc
    13261 tttgttacca ccttcgtatt gggtggagga gttccatgcg ccttcgatat cgtcgatagc
    13321 aacgtgggaa ccggagatag atgatccgaa acggatgtca tcgaacagga agaattcagg
    13381 ttctggcccg aacagtacgg tgtcggcaat gccagtggaa cgcaggtaat cttcggcgcg
    13441 cttcgcaatg gagcgcgggt cacggtcata gccttgcagg gtgccaggtt caaggatgtc
    13501 gcaacggata atcagggtgg agtcggcgaa gaacgggtca atcactgcgg tggatgcgtc
    13561 tggcatcagc accatgtcgg actcgttaat gcctttccag ccgccaatcg aggagccgtc
    13621 aaacattttg ccttcttcga agaattcagc attcacctga tgagcaggga tagtgacgtg
    13681 ctgttcttta cctttagtat cggtgaagcg caaatcaaca aacttcactt cgtgctcgtt
    13741 cagcatcgtc agtacgtgtt cagcggacat actttaactc tcctggattg gtcatggtcg
    13801 tcgtggtaac gaaatctgca attattttgg ccgtgtcgcc gtaaaaaaga taaagcgaaa
    13861 tctgtgccaa cttttaaatt gcccctaaaa ggcgttatca tgcgcaccat cgtgcaaaag
    13921 ggctgcacca cgatgtgaat gttgcaccaa tatagtgctt caatggaaac attaagcacc
    13981 atgttggtgc aatgaccttt ggataaccct ttttatgctc cgtgaaagcg atcacaaagg
    14041 gactctgcaa tacttgtttg cggaggatgt ttgtgatcct gttttgtagt gcgattaatc
    14101 cgtgtacaat aacgcgctat ttctaatgcc tgaggcaaag ttgtgatcga aaaattgcgt
    14161 aatatcgcca tcatcgcgca cgtagaccat ggtaaaacca ccctggtaga caagctgctc
    14221 caacaatccg gtacgttcga ctctcgtgcc gaaacccaag agcgcgtgat ggactccaac
    14281 gatttggaga aagagcgtgg gattaccatc ctcgcgaaaa acaccgctat caaatggaat
    14341 gattaccgta tcaacatcgt tgataccccg gggcacgccg acttcggtgg tgaagttgaa
    14401 cgtgtaatgt ccatggtaga ctcagtgctg ctggtggttg acgcatttga cggcccgatg
    14461 ccgcaaacgc gcttcgtaac caaaaaagcg tttgcttacg gcctgaagcc gattgttgtt
    14521 atcaacaaag ttgaccgccc tggcgcgcgt cctgattggg ttgtggatca ggtattcgat
    14581 ctgttcgtta acctcgacgc gaccgacgag cagctggact tcccgatcgt ttacgcttct
    14641 gcgctgaacg gtatcgcggg tctggaccac gaagatatgg cggaagacat gaccccgctg
    14701 taccaggcga ttgttgacca cgttcctgcg ccggacgttg accttgacgg tccgttccag
    14761 atgcagattt ctcagctcga ttacaacagc tatgttggcg ttatcggcat tggccgcatc
    14821 aagcgcggta aagtgaagcc gaaccagcag gtcactatca tcgatagcga aggcaaaacc
    14881 cgcaacgcga aagtcggtaa agtgctgggc cacctcggtc tggaacgtat cgaaaccgat
    14941 ctggcggaag ctggcgatat cgtggcgatc acgggccttg gcgaactgaa catttctgac
    15001 accgtttgcg acacgcaaaa cgttgaagcg ctgccggcac tctccgttga tgagccgacc
    15061 gtttctatgt tcttctgcgt taacacctcg ccgttctgcg gtaaagaagg taagttcgta
    15121 acgtctcgtc agatcctgga tcgtctgaac aaagaactgg tacacaacgt tgcgctgcgc
    15181 gtagaagaaa ccgaagacgc cgatgcgttc cgcgtttctg gtcgtggcga actgcacctg
    15241 tctgttctga tcgaaaacat gcgtcgtgaa ggtttcgaac tggcggtatc ccgtccaaaa
    15301 gtaatcttcc gtgaaatcga cggtcgtaaa caagagccgt atgaaaacgt gactctggac
    15361 gttgaagaac agcatcaggg ttctgtaatg caggcgctgg gcgaacgtaa aggcgacctg
    15421 aaaaacatga atccagacgg taaaggccgc gtacgtctcg actacgtgat cccaagccgt
    15481 ggtctgattg gcttccgttc tgagttcatg accatgactt ccggtactgg tctgctgtac
    15541 tccaccttca gccactacga cgacgtacgt ccgggtgaag tgggtcagcg tcagaacggc
    15601 gtactgatct ctaacggtca gggtaaagcg gtcgcgttcg cactgttcgg tctgcaggac
    15661 cgcggtaagc tgttcctcgg tcacggtgca gaagtttacg aaggtcagat tatcggtatt
    15721 cacagccgct ctaacgacct gactgtaaac tgcctgaccg gtaagaaact gaccaacatg
    15781 cgtgcttccg gtactgacga agctgttgtt ctggttccgc ctatccgcat gactctggaa
    15841 caagctctgg agttcatcga tgatgacgaa ctggtagaag tgactccgac ctctatccgt
    15901 attcgtaaac gtcacctgac ggaaaacgat cgtcgtcgcg ccaaccgcgc accgaaagac
    15961 gattaatttc gttttttagt tataaaaaac ctgccatcgc t
//
LOCUS       DAZOZY010000085        15931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZOZY010000085 DAZOZY010000000
VERSION     DAZOZY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.085"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHURQ_002341"
     CDS             <1..173
                     /locus_tag="ACHURQ_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR0824096.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHURQ_002342"
     CDS             complement(345..674)
                     /locus_tag="ACHURQ_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR0824097.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHURQ_002343"
     CDS             complement(679..864)
                     /locus_tag="ACHURQ_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR0824098.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHURQ_002344"
     CDS             complement(861..3500)
                     /locus_tag="ACHURQ_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR0824099.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHURQ_002345"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHURQ_002345"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0824100.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHURQ_002346"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHURQ_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR0824101.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHURQ_002347"
     CDS             complement(5227..5493)
                     /locus_tag="ACHURQ_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR0824102.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVALGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHURQ_002348"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHURQ_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR0824103.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9657..10790
                     /gene="ompN"
                     /locus_tag="ACHURQ_002349"
     CDS             9657..10790
                     /gene="ompN"
                     /locus_tag="ACHURQ_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR0824104.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10931..11365
                     /gene="uspF"
                     /locus_tag="ACHURQ_002350"
     CDS             10931..11365
                     /gene="uspF"
                     /locus_tag="ACHURQ_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR0824105.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11951..12865
                     /gene="sitA"
                     /locus_tag="ACHURQ_002351"
     CDS             11951..12865
                     /gene="sitA"
                     /locus_tag="ACHURQ_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR0824106.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12865..13692
                     /gene="sitB"
                     /locus_tag="ACHURQ_002352"
     CDS             12865..13692
                     /gene="sitB"
                     /locus_tag="ACHURQ_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR0824107.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13689..14546
                     /gene="sitC"
                     /locus_tag="ACHURQ_002353"
     CDS             13689..14546
                     /gene="sitC"
                     /locus_tag="ACHURQ_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR0824108.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14543..15400
                     /gene="sitD"
                     /locus_tag="ACHURQ_002354"
     CDS             14543..15400
                     /gene="sitD"
                     /locus_tag="ACHURQ_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR0824109.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15601..>15931)
                     /locus_tag="ACHURQ_002355"
     CDS             complement(15601..>15931)
                     /locus_tag="ACHURQ_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0824110.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3986 a         4023 c         3829 g         4093 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctag
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9481 tattatcttc gatacattca ccagacttat tatcatttca gaagaattat ttgattaagg
     9541 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
     9601 tttcactccc cggaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
     9661 aaagcaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag
     9721 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
     9781 attatttttc tgataattca gcgaaagatg gcgatcagag ctatgcgcgt ctgggtttta
     9841 aaggcgaaac ccaaattaac gatcaactca ctggctacgg tcaatgggaa tacaatattc
     9901 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccgggc
     9961 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
    10021 tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca
    10081 actttatgac tggtcgtgcc aacggcgtcg cgacttatcg taatactgat ttcttcggtc
    10141 tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaaggt gccagtaatg
    10201 gtcaggaagg caccaacaac ggacgtgatg ttcgccatga aaatggtgac ggctggggtc
    10261 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcatctg
    10321 accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
    10381 ctgctgggct aaaatacgat gctaacaata tttacctggc aaccatgtat tcagaaacgc
    10441 gtaatatgac cccgtttggc gacagcgatt atgctgttgc aaacaaaacc cagaactttg
    10501 aagtcactgc acagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
    10561 ctaaaggccg tgacctgcac gctgccgatg gtgcagacaa cccggcaggt gttgatgata
    10621 aagatcttgt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
    10681 cttatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg
    10741 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
    10801 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
    10861 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
    10921 aggaggtttt atgaacagaa cgattcttgt ccctatcgat atttccgatt cagaattaac
    10981 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gacgatgcag aggttcattt
    11041 cctgacggta ataccttcac tgccttacta tgcctctctg ggtttagcgt attccgcaga
    11101 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
    11161 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
    11221 ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
    11281 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
    11341 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
    11401 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt
    11461 atatcagatt tcttatgctg ggcgttccgg catgacgcat actcttctga tgccatataa
    11521 cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa
    11581 cctgaacaag taagggcaaa aatcacaact atctgaccca taagtatttt atttattttt
    11641 caatatgtta aacattatca gcgacaacaa taagctacga taacccactc tttttttgcc
    11701 ccttttttgc cccttctaca gcgttttgcc ccatttttgc catcaacaat tttcctcata
    11761 aagtcatttc aaagcctaat agctgctcta cttttttcct caatatcatg tcgccagcag
    11821 gaaacaacct aatccctgtt ttaggcctca aagctgttaa tgataatcat tttcattttg
    11881 tatgggttat gctataaaac atagccgttg ctatgtttgt tctaattttg ctcactatag
    11941 gtactaaatt atgcactcaa taaaaaaagt aaccatgctc ttggggtgcc tcgccctcac
    12001 ctgctcgatc gcatttcagg caagtgcaac tgaaaaattc aaggtcatta caacatttac
    12061 catcatcgcg gatatggcca aaaacgtggc tggagatgct gcagaagtct catccataac
    12121 caagcctggt gcagaaattc atgagtatca gcctacccct ggcgatatta aacgtgcgca
    12181 gggggcacaa ctgattctcg ccaatggtat gaatctggaa ttgtggttcc aacgctttta
    12241 ccagcatctc aatggggttc cagaagtaat tgtctcttcg ggtgtgacgc cagtagggat
    12301 caccgaagga ccctatgagg gcaaacctaa tccccatgca tggatgtcgc cagataatgc
    12361 tctgatttac gtcgataata ttcgtgatgc gttgataaaa tacgatccgg caaatgcaca
    12421 aacctaccaa cgtaatgccg atacttataa agccaagatt acccaaaccc ttgcccccct
    12481 gcgtaagcag attacggaac tccctgagaa tcagcgatgg atggtcacca gtgaaggggc
    12541 tttttcttat ctcgcacgcg atttggggct aaaagagctt tatctgtggc cgattaatgc
    12601 cgatcaacaa ggaacaccgc agcaggtacg taaggttgtt gatatagtta agaaaaatca
    12661 tatcccggca gtctttagcg agagtacgat ttccgataaa ccagcgcgtc aggttgcgcg
    12721 tgaaaccggc gcacactacg gcggtgtgct ctatgtcgat tccctgagca cagaaaacgg
    12781 cccggtacct acgtatatcg accttcttaa ggttactacc agtacgctgg tacagggaat
    12841 taaagccggg aaaagggaga aataatgatg caatctgcag gcattgtcgt taatgatgtt
    12901 actgtcacct ggcgtaacgg gcacacagca ctgcgcgatg catccttcac agtaccaagt
    12961 ggatctattg ccgctctggt tggggtaaac ggttccggaa aatcgacgtt gtttaaagcg
    13021 attatgggat ttgtgcgtct gaccagcgga aagatatctg ttctgggtat tcccacgcga
    13081 caggcgctac agaaaaacct ggttgcctac gtccctcagt cagaagaggt tgactggtca
    13141 tttcctgtac tggtggaaga tgtggtgatg atgggccgct atgggcatat gggtatgttg
    13201 cgtatagcca aaaagaggga tcgtcagatt gtcaccgatg cgctggaacg tgtcgatatg
    13261 gtggattttc gccatcgtca aatcggcgag ctttccggtg gacaaaagaa acgcgtcttt
    13321 ctggcgagag cgattgcaca gcagggtgat gtgatcctgc tggatgaacc ttttaccggc
    13381 gttgatgtga aaaccgaagc gaaaatcatc agcctgttga gggagttacg cgcggaaggc
    13441 aaaacgatgc ttgtctcaac gcacaatctc ggctcagtca cgacattttg cgattatacg
    13501 gtcatggtca aaggcaccgt cctggcaagc gggccgacag acaccacttt tacagccgag
    13561 aacctggagc tggcttttag cggcgtactg cgccatgtca cacttaacga ctcggaagaa
    13621 agcattatta ctgacgatga acgccctttt gtggcacatc gaccgtcagc ggtgcagagg
    13681 gaagcaagat gaacgtgcta ctggaaccct tcagctatga atatatgctc aatgcgatgt
    13741 gggtctcggc aatggtcggc ggtctctgcg ctttcctgtc atgctatctg atgctaaaag
    13801 gctggtcgct gattggcgat gcgctgtcgc actctattgt cccgggtgtt gcgggggcat
    13861 atatgctggg gcttccgttt tctctgggag ccttcttttc cggcggactg gcagcgggca
    13921 gcatgctgtt tcttaaccaa cgcacccgct taaaagaaga tgccatcatc ggcctgatct
    13981 tctcgtcctt ttttggcctg gggctgttta tggtgtcatt gaacccgaca tccgtgaaca
    14041 ttcagaccat cgtcctgggc aatattctgg caatcgatcc ggcggatatc ctgcaactca
    14101 ctatcattgg cattctctcg atcatcgtgc tttttttcaa atggaaagat cttatggtga
    14161 cctttttcga cgagaatcac gcccgtgcta ttggattaca tccgggcaga ttaaaaatcc
    14221 tcttcttcac cctgctgtcg gtttcgaccg ttgcggcgct gcaaacggtt ggtgcctttc
    14281 tggtgatctg tctggtggtg actcccggtg caacagcatg gttgttaacc gatcgttttc
    14341 cccgcctgct cataattgct gtaactattg gcagtgtaac cagtttcctc ggcgcgtggg
    14401 tcagttactt tctggacggc gcgacaggtg gaatcatcgt ggtggcgcag acactgctgt
    14461 ttcttttagc atttgtattt gcgcccaccc atggactttt ggcaaatcgt cggcgtgcgc
    14521 ataaagcgct ggaggatcgc tcatgatggc attattactg gagccactgc agttcacctt
    14581 tatgagtcac gcactgctga tttcactggt agtttccata ccctgtgcgc tgctgtcggt
    14641 ctttctggtc ctgaaaggtt gggcactgat gggtgacgcg atgagtcatg cggtttttcc
    14701 tggcatcgtg cttacctgga ttctggggtt acctctggct acaggggcat tcgtcgccgg
    14761 agtattctgc gcggtggcga cgggatacct gaaagacaac agccgaatca agcaagatac
    14821 agttatgggg attgtttttt ctggcatgtt tgccgcaggt ctgatcttgt atattgcaat
    14881 caaaccagac gtacatcttg accatattct tttcggcgac atgctcggga taaccatcgg
    14941 cgatataatc cagacggtga ttattgccgg gctggttaca cttgttatta gcgcaaaatg
    15001 gcgggatttt ttgctgttca gctttgatta tcaacaggcg caggtaagcg gtttgcatac
    15061 gagatggctg cattatgggc tgctgtgcat ggtttccctg accattgtgg cgacgctgaa
    15121 agcagtaggt atcattctct ctatttctct gctcattgcg cctggcgcca ttgcagtact
    15181 cctgacacaa cgctttcata ttgcgctcct actggcgact ggcatatcgg taatagtgtc
    15241 aatgactggt gtctggctgt ccttttttat cgacagcgca ccagctccga cgattgtcgt
    15301 cttattcgcg gtcgtgttca tcatgacgtt taccgttacc agcatcaacg cacgcacaaa
    15361 gggaaacgcc catacacagg atctgttatc acccaattag cacaacatcc gaggacagca
    15421 tcgtccgcca gcctacagaa gtccggaaag ccatggaagg tcgttgggag gaggtacgaa
    15481 ttctatcatg caaaaaatac gtaaaatcga taacgcctag aaatcattca atactcgcac
    15541 tatcgaaagt ttaccagcca accgcggcac gttcctgcat acgacgtgcc gcagcttctg
    15601 ttattccacg ataaaattcc taaccgaatt caaatccttc agactattca gctcttcctt
    15661 cagctcccgc tgacggcgat aaatctcgtc attgcgctca acctgtgcct gagccattgc
    15721 tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat cggcatcacc
    15781 ccacgtcatc acgtcccgta caatgtcgga tttcgcagcc attaccaccg gataaaggcg
    15841 gcccagtgag ttggggccag cattccagat acgaccgttc cattcaaacg tgaacggctt
    15901 cgcctcctgt tctgtgcgcc atgcttcaat t
//
LOCUS       DAZOZY010000086        15581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZOZY010000086 DAZOZY010000000
VERSION     DAZOZY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.086"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHURQ_002356"
     CDS             92..1132
                     /locus_tag="ACHURQ_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR0824111.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHURQ_002357"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHURQ_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR0824112.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHURQ_002358"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHURQ_002358"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR0824113.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHURQ_002359"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHURQ_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR0824114.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHURQ_002360"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHURQ_002360"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR0824115.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHURQ_002361"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHURQ_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR0824116.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHURQ_002362"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHURQ_002362"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR0824117.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHURQ_002363"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHURQ_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR0824118.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHURQ_002364"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHURQ_002364"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR0824119.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11786..12622)
                     /gene="kduI"
                     /locus_tag="ACHURQ_002365"
     CDS             complement(11786..12622)
                     /gene="kduI"
                     /locus_tag="ACHURQ_002365"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR0824120.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12909..14090)
                     /locus_tag="ACHURQ_002366"
     CDS             complement(12909..14090)
                     /locus_tag="ACHURQ_002366"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR0824121.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14345..15574
                     /locus_tag="ACHURQ_002367"
     CDS             14345..15574
                     /locus_tag="ACHURQ_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0824122.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         4009 a         4201 c         3683 g         3688 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccaca tggtccatat
    11821 catcaaagac ctggttttca ccaaccatgc cccagataaa ggtataagct ttggttccga
    11881 cgccggaatg gatcgaccag ctgggggaga tcaccgcctg ctcgttatgc atcacaatgt
    11941 gacgcgtttc ttgcggctgc cccatcatgt ggaaaacgca ggcgtcatca tccatattga
    12001 aatagaaata aacttccatc cggcgctcgt gagtgtgaca cggcatggtg ttccacaagt
    12061 tacctggagc cagctccgtc agccccatac tcaattggca ggtttccagc acatccggga
    12121 caaaatattt gttaattgtg cgacggttac tggtgaggtt atcgcctaac gtgactggag
    12181 atacttcgtc cggtgtgact tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt
    12241 aataaaactt tgccggagtg gcggtatcaa tactggcaaa gacaacttct tttgcacctt
    12301 taccaacata cagggcgtcg cggtgaccga tttcatagca ttggccatcg acagtaatcg
    12361 taccggcacc gccaatattg ataacaccta actcgcgacg ttcaaggaaa tagcttacgc
    12421 cgagttgttt accaacttcc ccgccaacgg aaaccgtttt agttatcggc ataatgccgc
    12481 caacaataat tcggtcaatg tggctgtaaa ccatggtgta ctcatcggcg acaaatacct
    12541 tttcaaccaa aaattcattg cgcagccctt gggtatccag cgtttttgcg tgcgcactgt
    12601 ggatgccctg tcttacgtcc acatcaacct ccgataatca gtgaagcgtg aaaatagaaa
    12661 acgatagtga aacactgttt cgttttctct ataaagggat gatatgccca tgggctaggg
    12721 ttttcaatta aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt
    12781 gtcgatcacg aatatgtcat gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga
    12841 cttgccagcc atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa
    12901 atgcagagct actcgtcacg ttcaatggtc aatgccacgc cctggccccc gccgatacaa
    12961 agcgttgcca gccctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg
    13021 attcggcaac cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg
    13081 acccgacgct catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct
    13141 tcattagcct cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg
    13201 cgggtcgcat acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat
    13261 gcgcggatgc gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc
    13321 attaccgcag ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga
    13381 ctatcaaatg aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt
    13441 tcatcggtat caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca
    13501 tctttaaatc gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa
    13561 gcatcctgca actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca
    13621 atatgataat cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg
    13681 ctattgccaa gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg
    13741 ttttcctggc caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc
    13801 agatgcagtg ctttaagtcc ggaaccgcaa acgtcattaa tcgtgattgc agaaacgcta
    13861 ttaggaagac cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc
    13921 aacacctgac caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt
    13981 aacgctttca cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccacga
    14041 aagcagccga taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg
    14101 caccaattaa cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa
    14161 aaagtgattt ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac
    14221 gtaaggtttg caaccactaa cccaccaata gatgggtaga taaggcgtta atctcccata
    14281 cttaacctgg tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca
    14341 gataatgtct aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac
    14401 agccgttggt gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt
    14461 cctgtttatt actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg
    14521 ccagttcatc ctctcatcaa aaacatcagc atgtgaaggg ataacgggcg cggtaacaca
    14581 ctactatggc aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt
    14641 cgtcgtgttg atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca
    14701 tatggttatt gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa
    14761 tctcattttt ctgatgggac gtcatgccac tattcgggta atgggatttt tggtattccc
    14821 attgattgcc tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct
    14881 attaacgacc caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc
    14941 cgtgatggtt ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg
    15001 tgaaaaatat ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct
    15061 catcatctgc ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc
    15121 gtatattgaa gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc
    15181 gaacgcccca gcatggttgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa
    15241 atcattcctg ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac
    15301 actacagcag gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt
    15361 ggtatcgctg attaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta
    15421 tgcgatcagc ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta
    15481 tctgatcccg gcgcttaaac cctggcgctc catcggaaat ctgattacgc tgatcgtggg
    15541 tatcctgtgc gtatcggtaa tgttctttag ctaatatccg t
//
LOCUS       DAZOZY010000087        15574 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZOZY010000087 DAZOZY010000000
VERSION     DAZOZY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.087"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            105..377
                     /locus_tag="ACHURQ_002368"
     CDS             105..377
                     /locus_tag="ACHURQ_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR0824123.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(510..1382)
                     /gene="rluF"
                     /locus_tag="ACHURQ_002369"
     CDS             complement(510..1382)
                     /gene="rluF"
                     /locus_tag="ACHURQ_002369"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR0824124.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1730..2567
                     /locus_tag="ACHURQ_002370"
                     /pseudo
     CDS             1730..2567
                     /locus_tag="ACHURQ_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            2637..3443
                     /locus_tag="ACHURQ_002371"
     CDS             2637..3443
                     /locus_tag="ACHURQ_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0824125.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3453..3860
                     /locus_tag="ACHURQ_002372"
     CDS             3453..3860
                     /locus_tag="ACHURQ_002372"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR0824126.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            3860..4354
                     /locus_tag="ACHURQ_002373"
                     /pseudo
     CDS             3860..4354
                     /locus_tag="ACHURQ_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            4420..5217
                     /locus_tag="ACHURQ_002374"
     CDS             4420..5217
                     /locus_tag="ACHURQ_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR0824127.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            5228..6052
                     /locus_tag="ACHURQ_002375"
     CDS             5228..6052
                     /locus_tag="ACHURQ_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR0824128.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6104..7332
                     /gene="sorE"
                     /locus_tag="ACHURQ_002376"
                     /pseudo
     CDS             6104..7332
                     /gene="sorE"
                     /locus_tag="ACHURQ_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            7715..8404
                     /gene="pepE"
                     /locus_tag="ACHURQ_002377"
     CDS             7715..8404
                     /gene="pepE"
                     /locus_tag="ACHURQ_002377"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR0824129.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8495..10126)
                     /locus_tag="ACHURQ_002378"
     CDS             complement(8495..10126)
                     /locus_tag="ACHURQ_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR0824130.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10346..14029)
                     /gene="metH"
                     /locus_tag="ACHURQ_002379"
     CDS             complement(10346..14029)
                     /gene="metH"
                     /locus_tag="ACHURQ_002379"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR0824131.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14229..15053
                     /gene="iclR"
                     /locus_tag="ACHURQ_002380"
     CDS             14229..15053
                     /gene="iclR"
                     /locus_tag="ACHURQ_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR0824132.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15370..>15574
                     /locus_tag="ACHURQ_002381"
     CDS             15370..>15574
                     /locus_tag="ACHURQ_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824133.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDF"
BASE COUNT         3732 a         4080 c         4047 g         3715 t
ORIGIN      
        1 tcgaaagaga taaatagtta agagaaggca aaatgaatat tatcagttct gctcgcaaac
       61 gaattcccgt acactgaact ctctggctca ctgagggcac catcatggca ctcccccgta
      121 ttacccaaaa agagatgact gaacgcgagc agcgtgaact taaaacactg ctggatcggg
      181 cgcggattgc gcatggtcgc gtactgacca actccgaaac caacagcatt aagaaagagt
      241 acatcgataa attaatggtc gagcgtgagg ctgaagcgaa aaaagcccgc caattgaaga
      301 aaaagcaggc ttataaaccg gatcctgaag cgtcatttag ctggtcagca aatacgtcga
      361 cgcgcggcag gcgctaatga acaatcaacg cctgagattt tcactcgtca gtgtgaacac
      421 aattttcccc tcaccctaac ccttttcccg aaggggcgag gggactgtcc gagcactttt
      481 gggccgtttt catcagtctg aggcgttaat cagcgcccct tctttttacg ccccggcgag
      541 gtaaaacgct taccgttgga cgccgggcga ccgccttttt ccgccgtttt ttccatctta
      601 acgactggac gtttgatgcc cgctgttttc ggtttggcct tcgctttggg cttcgcctcg
      661 gaagaggaat tttcaatgag cttaaagaga tcgataagct cgtcgtcggt taaatcgcgc
      721 cattctccca gcggaatgcc gcttaagctg acgttcataa tgcgcgtacg ttccagcttt
      781 ttcacttcat agccgaaatg ctcgcacatg cgccggatct gacggttcaa cccctgcacc
      841 agggtaatgc ggaagacaaa cggtgcttct tttttgactt tgcacttttt cgtcaccgtc
      901 ccgaggatcg gcactcccgc ccccatacca cgaataaatt catcggtaat cggtttatcg
      961 accgtcacca gatactcttt ctcatgatcg ttacccgcac ggaggatctt attcaccaga
     1021 tcgccgtgat tggtgaggaa aatcagcccc tgggagtctt tatccaggcg gccaatcggg
     1081 aacacgcgtt tgctgtggtt aacgaaatcg acaatgttat cgcgctcgcc atcttcggtg
     1141 gtgctgacaa taccgacggg cttgttcagg gcgataagta ccaaatcttc ggcttcccga
     1201 ggctcaatca actgaccgtt tactttcaca atgtcgccgg gtttcacctg atcaccaatg
     1261 gtggctcgct tgccattaag gaacacattg ccttgctcga tatagcgatc cgcttcgcgg
     1321 cgtgagcaaa ttccgctttc gctgatgtat ttatttaaac ggactgatga gtcgggcagc
     1381 ataaattctc ctgtaaaacg cggaatatac cgtacctctt agcggggaaa aacagggtta
     1441 tgcggcaacg tcgaaattaa atgtgatctg ccacgccttt tatcacaaaa gtgtctccgg
     1501 aagtacgatg caaatttgcc tggcaaaata aggataatca acatattacg aatcgctgca
     1561 cacctgtgct gatgactact acgcagagaa acaaatgtgc aagagaaagc gctatttctc
     1621 cccatgcaaa gagtgaaaaa tcgctttgaa aggccctcat aatggggtta tatcagatca
     1681 agcggtgaga aagcgtattt gcacaaatgt gcagaaccag gaggcggcaa tggaaaacag
     1741 tgacgatatc cgtttgattg tgaagattgc ccaactctat tacgaacagg atatgacgca
     1801 ggcgcaaatc gcgcgcgaac tgggtattta ccgcaccacc atcagccgct tgcttaaacg
     1861 aggccgcgat cagggaattg tcaccatcgc catcaactat gactacaacg aaaatctctg
     1921 gctggagcag caactgaagc aaaagtttgg cttgaaagac gttgtggtgg tgtcgggaaa
     1981 tgatgaggat gaagagactc aactggcgat gatggggtta cacggcgcgc aactgctgga
     2041 tcgcttgctg gagcctggcg atattgtcgg tttttcctag ggtcgcgcgg tgagcgcact
     2101 ggttgaaaac ttgccgcaag cggggcaatc gcggcagtta atctgcgtgc caattattgg
     2161 cggaccgtcc ggtaaactcg aaagccgcta tcacgtaaac acattaacct acagcgtggc
     2221 agcgaagctg aaaggggaat cgcatctcgc ggattttccg gctctgctgg ataacccctt
     2281 aattcgtaat gggatcatgc agtctcagca ctttaaaacc atctctgcct actgggataa
     2341 tctggatgtc gccctggtgg gaattggctc accggccatt cgcgacggcg ctaactggca
     2401 tgcgttttat ggtggtgaag agagtgacga cctgaaagca ggcgcggtat tccattggca
     2461 ttgccatgag tgaagaaaaa tacagcggaa ttgttggtgc actgcgtgga aaatatatta
     2521 attgtctggt aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat
     2581 gtttaccgta ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc
     2641 aaacgtggtt aaatttgcag gataaaatca ttatcgtcac cggcggcgca tccggtattg
     2701 gtctggcgat tgtagaggaa ttattagcac aaggcgcaaa cgtacagatg gtagatattc
     2761 acggtggcga tggtcaatat gaagttcata aaggctatca gttttggccg accgatattt
     2821 ccagcgccaa agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg
     2881 acggtctggt caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc
     2941 ctgccgggca gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaaa
     3001 aaggcgtttt tctgatgtcg caggcggtgg cgcgccagat ggtcaaacaa catgatggcg
     3061 tgattgtgaa tgtttcctca gaaagtggac tggaaggctc agaaggccaa agctgttacg
     3121 ccgctaccaa agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac
     3181 acggtatccg tgtggtcggt atcgcgccgg ggattctgga aaaaaccggg ctacgcacgc
     3241 cggaatatga agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag
     3301 gctataccaa aaacgccatt cctattgggc gcgccggaag attagcagaa gtcgctgatt
     3361 ttgtttgtta tctgctgtct gaacgcgcca gctacatcac cggagtaacc actaacattg
     3421 cgggcggcaa aacgcgcgga taaggaggct ttatggtcaa cgccattttt tgtgcccacg
     3481 gcaaattagc ttgcgccatg ctggaatcgg ttcagatggt gtacggcgac gccaatgtcg
     3541 aggcggttga atttgtacca ggcgagaacg ccggagacat cgtagcaaag ctggaaaagt
     3601 tagtaagcat tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc
     3661 cgtggaatgc ggcagcgatg cttgcgatgc gcaatcctcg tctgcgggta atcagcggcc
     3721 tttccctgcc gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac
     3781 tgtgtgaaca cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa
     3841 cggcagagga agatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca
     3901 cggccaggtc actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa
     3961 tgacgaagtt tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg
     4021 aatgaaggtt aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata
     4081 ttaggacgaa accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg
     4141 ccagggggta aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa
     4201 acagttaacc aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa
     4261 taatcttggc gtcataatgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat
     4321 cgacaaaata aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt
     4381 taacaggcag ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa
     4441 attattgcta tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt
     4501 cagactcatc gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa
     4561 accggaataa tactcggtgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc
     4621 gcggcgcaat ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt
     4681 ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccaa
     4741 gtgctgacgg tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca
     4801 gcggaagagg cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag
     4861 gcgctgcgcg ttgctattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc
     4921 agcaatatgt taaacgccat tcccgaattt gtgacccgtg gactgcaaat tgctggcggt
     4981 tttatcgtgg tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg
     5041 cctttctttt tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc
     5101 ggtggcgtcg gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa
     5161 gctgaacctc aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg
     5221 agccatcatg gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc
     5281 caacctgcaa caggcgtcct ttaactttga acgtattcac ggactgggct tttgctacga
     5341 catgatcccc gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag
     5401 gcgacacctg gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgtcac
     5461 tgccgccatg gaagaggcgc gggccaacgg cgcggaaatt gatgacggta ccattaacgg
     5521 catcaaagtc ggtctgatgg gaccattggc aggagttggc gatccactgg tctggggaac
     5581 gctgcgcccg attaccgctg cgctcggcgc atctctggca ctttctggca acatcctcgg
     5641 cccgctgctg tttttcttta ttttcaatgc ggtgcgtctg gcgatgaagt ggtatggcct
     5701 acagctcggc tttcgcaaag gggtgaatat cgtcagcgat atgggcggga atgtgctgca
     5761 aaaactcacc gaaggcgcgt cgattctcgg actgtttgtg atgggcgtgc tggtcaccaa
     5821 atggacgtca atcaacgtac cgttggtggt ttcacaaacg catgccgccg atggctccac
     5881 cgtcatcatg accgtgcaga acattctcga ccaactttgc cctggtttgc tggcgctcgg
     5941 tctgacgcta ctgatggttc gtctgctcaa caaaaaaatt aacccggtat ggctgatttt
     6001 cgccctgttt ggcttaggga ttatcggcaa tgcgctgggc ttcctgtcct gattcttcgc
     6061 cccggcacga ctgccggggc catcgctcaa catgaggtgg tttatgaaaa caacagctct
     6121 gcgtctttat ggtaaacgtg atttacgcct ggaaaccttt gaccttcctg aaatgcagga
     6181 ggatgaaatc ctcgcgacgg tggtcactga cagcctgtgc ctctcttcct ggaaagaggc
     6241 aacctgggtg aaaaccataa aaaagtaccc gacgatgtgg cgaccaaccc aatcatcatc
     6301 ggccacgagt tttgcggcga tattctggcc gtgggtaaaa agtggcagca caaattccag
     6361 ccgggtcagc gttatgtgat tcaggccaac ctgaaactcc ctgaccgccc ggactgcccc
     6421 ggctactcct tcccgtgggt gggcggcgaa gccacgcatg tggttattcc caacgaggtc
     6481 atggaacaag attgcctgct ggcatacgac ggcgaaacct attttgaagg ctcgctggtt
     6541 gaaccgcttt cctgcgtgat tggcgcgttc aacgccaact atcatcttca ggaaggtagt
     6601 tataaccaca cgatggggat tcgcccgcaa gggcgcacgc tgatcctcgg cggcaccgga
     6661 ccaatgggat tgttggcgat tgattatgcg ctgcatggac cagttaaccc gtcgctgctc
     6721 gtcattaccg ataccgacaa cgacaaattg agttatgcgc gcaagcacta tccatcagaa
     6781 ccgcaaacac tgattcatta tctcaatgcc accgatgcag catttgatac gttaatggcg
     6841 ctgagtggcg gtcacggctt cgatgatatt ttcgtctttg tgcctaatga aggactggtg
     6901 actctcgcct cttccttgct ggcgacagat ggttgcctga atttcttcgc cggaccgcag
     6961 gataaacatt tcagcgcgcc aattaatttc tacgatgtgc attatgcatt tacccactac
     7021 gtgggcacgt caggcggcaa taccgacgac atgcgcgcgg cggtcaaact gattgaagag
     7081 aaaaaagtgc aggccgcaaa agtggtaaca catattcttg ggctgaatgc cgcgggcgaa
     7141 accacgcttg aattgcctgc cgtcggcggc ggcaaaaagc tggtgtatac cgggaaatac
     7201 ctgccgctga cgtcactcac gcagattcag gatcaagcac tggcggcgat tctggcgcgt
     7261 catcagggga tctggtccgg tgaggcggag caatatctgc tcactcatgc agaggcaatt
     7321 tcccatgatt aatcgcgcta cttaactctg catgtcgctg gctggtcgcc gcggcaactt
     7381 cgcgacctga tgtgcggctg attgctgaag cggcccaatg tgcacgagaa ggataaaaaa
     7441 acatcagcgc cttaaggggc gctcaaattg gtggcaaagt acacattgtt tatgccggat
     7501 gcggaataaa gccttattcg gcctacataa tctcgaaaat tcaatatatt gcagttatct
     7561 ggtaggcctg gtaagcgtag cgcatcaggc aatgctgtgt ttgtcatcag tctcagcgcc
     7621 cggtgcaata cgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca
     7681 tgccttccgt gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc
     7741 gacgctgccg ggtaaagcct ggctggaaca tgcactgccg ctaattgctg aacagttgca
     7801 gggtcgccgc tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta
     7861 cacagcgaaa acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag
     7921 cgttgtcgat cccgttgccg cgattgaaaa tgctgagatc gtgattgtcg ggggcgggaa
     7981 tactttccag ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt
     8041 ggttaaacgt ggtgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac
     8101 cattcgtacc accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct
     8161 gttcccgctg caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga
     8221 aacccgtgag cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg
     8281 tctaccggaa ggtaactgga tcacggtgag taaaggtcac gctacgctgg gtggcccgaa
     8341 caccacttat gtgtttaagg ccggtgaaga agcggtgccg ctggaagcgg gtcaccgttt
     8401 ttaagtgaat tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt
     8461 gacgcgtctt atcaggccta caggattcac aatattaata ctcgtcccgt ccttcatctt
     8521 catccggctg ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc agacgctgca
     8581 tatctcccag taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat
     8641 gcaggcgatc gacgtgggcg tgggaatagc ggcgattaag aatgcgaaag cgatgcttgc
     8701 tgcggcgcaa acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagct
     8761 ttaaattact gagcaattgc tcatagagcg catccagttc cttcaaccca tcaagcgaaa
     8821 atgcccgccg tgctgccagc gatttatcag caatttcgct gcccatgcgc tcgacgatat
     8881 cggaagcctg ttcaaggttg agcgacattt caataatctc cgcccagcga cgcgactctt
     8941 cttctgccag ttcctctttt ggcattcgcg ccagatacag cttaatggcg gtgtagagaa
     9001 cgttgatatc atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca
     9061 ctttattcag cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg
     9121 cggcgttcgc cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc
     9181 gtagctgggt atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg
     9241 ggtcaacaaa tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga
     9301 tcaccagttc cgcttttggc aatgacaact tccccattgt ctctgccagc aaatggacaa
     9361 acggcaggat aatcaggcta cccaccagct taaacagcaa actacccagc gcgacacggc
     9421 gggctgcggc attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag
     9481 caccaatcac cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg
     9541 cggtcagcag tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca
     9601 gcgcatccag cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct
     9661 gggtgatcgg cgttacggcc tgcacaatca actccagcgc cagcaaaatc agcccaagac
     9721 caataccgac gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca
     9781 cgccgataaa aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca
     9841 ttagcgccgt cccgacatct gcacccagca caatcaccag agccggtgcg agggctacca
     9901 gatcctgagc gacgaacgag gtcaccagca tggtggtggc attactgctc tgtaccagtg
     9961 cggtaacgcc gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga
    10021 cagtacgcaa acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc
    10081 agaccagcag ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcaggttctc
    10141 cttatcgtta tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg
    10201 gcatctttac tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc
    10261 cagtataagt gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg
    10321 taaggaaaag tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc
    10381 agcgctcaac atcggagacg ctcatacctt tacggcgggc atagtcttca acctgatcgc
    10441 gctgaatttg cgccaccgcg tagtacttgc tgtccgggtg gctgaaatac caacccgaaa
    10501 ccgatgcacc aggccacatg gcgaaagatt ctgtgagttt catgccagta tgtttttcca
    10561 cttccagcag ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg
    10621 gtgccggacg gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt
    10681 tcggcgcata gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt
    10741 cggctaaacg gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt
    10801 caaaggcatc agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt
    10861 aatctgcttt accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc
    10921 ccgttttttc ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt
    10981 cgtcacggta gatttcaatg tcatcgccca cacggtttgc cgggaacaag cccaccacgc
    11041 cacgcggatt cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa
    11101 acagccgctg cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg
    11161 ccagcgacca ggtcataaag aacggtgtcc agtcgatgta attacgcaga gtttcgatgc
    11221 tggcttcaac ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt
    11281 caaaagcgaa gtcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct
    11341 tgcgcccgtg ctgaatacgt acggtttcat actccttgcg ggtacgagcg acaaaatcat
    11401 cgcgctgagt atcggaaagc agcgccgcca ccacaccaac ggtgcgcgag gcattctgca
    11461 catacaccgt cgggccgctg tagttctgct caattttcac cgccgtgtgc gcttttgagg
    11521 tcgtcgcgcc accgatcagt aacggaatgg tgaagccctg acgctccatc tctttcgcca
    11581 cgttaaccat ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat
    11641 tcacttcttt agcggtacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa
    11701 tttcgtagtt gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt
    11761 cgcccttcac ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt
    11821 caataaacgg ttcgaggtag gccactgcct gtttcatgac gcgcgccgat ttgaccacct
    11881 gtggcaggaa cattttcccc tcgccaaaca gatcgccgac cacgttcatg ccgtccatca
    11941 acgggccttc aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct
    12001 gctcgataaa ctcagtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc
    12061 acgagcgcca ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt
    12121 tctcggcaag ctccagtaaa cgttcagtgc catcatcgcg acgattaaga atcacatctt
    12181 ccaccgcgtc gcgcagttca gcgggcaggt cgtcgtaaat cgccagttgc ccggcgttga
    12241 cgatccccat atccatgcca ttgcgaatag cgtagtagag gaacaccgcg tgaatggctt
    12301 cgcgcaccgg ttcgttgcca cggaatgaga aagaaacgtt agatacgccg ccggaaatca
    12361 gcgcgtgcgg cagttcgcgt ttgatgtctt cgcaggcgcc gataaagtcc tgcgcatagt
    12421 tgttgtgctc ttcaatgccg gtggcgaccg cgaagatgtt cgggtcgaag atgatatctt
    12481 ctggcgggaa gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt
    12541 tccgtgcgcg ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc accgctgcac
    12601 cgtaacgacg caacagtttc gcatgatgga taaaggcgtc gacgccctct ttcattgaga
    12661 tagagttaac aatgcctttg ccctgaatac acttcagccc cttttcgatg acctcccatt
    12721 ttgaggagtc gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga
    12781 gaaaacgcac catcgccgct tcggcgtcga gcatcccttc atccatattg atatcgataa
    12841 tctgcgcgcc gttttccacc tgctggcggg ccacatccag tgcctcgctg tatttctctt
    12901 ctttgatcag gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca
    12961 caaacaggct atcttcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg
    13021 ggatttccgg cagtttgcgc ggcgctaatc cttctactgc acgactcatc gctgcaatat
    13081 gttgtggcgt ggtgccacag cagccgccga cgatattgag aaagcccgct tccgcccatt
    13141 cacgtatttg ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta
    13201 gcccggcgtt cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt
    13261 actggcgcag ttcatcgggc cccagcgcac agttcaggcc aaaggtcagc gcttcggcgt
    13321 ggcgcaatga gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt
    13381 cggtgatggt gccagagatc ataatcggca gctcaacgcc cagcgtttca aactccgttt
    13441 ttaccgcaaa taccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca
    13501 gatccgcacc accttccacc agcgctttgg tggactctcg ataagccgcc accagcccgt
    13561 caaaagtgat attacgaaat gccggatcgt tgacgtccgg agaaatagac gccgtgcggt
    13621 tggttgggcc gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact
    13681 cgtcagcaca ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt
    13741 ccatctggta atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat
    13801 ccgcgcccgc ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttactgagta
    13861 ccagcaggtc gttgttgcct ttgaggtcgc atggccagtc ggcaaagcgt tcaccacgaa
    13921 aatcggcttc gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca
    13981 gaatacgttc atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg
    14041 acacgctcaa cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt
    14101 ggtaaaagcg accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc
    14161 agcaactatt gcattaacta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg
    14221 agactgtcat ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg
    14281 caccagcgac tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg
    14341 ccgaatccaa tggcagtgtg gcactcacgg aactggcgca acaagccggg ttacccaatt
    14401 ccacgaccca ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggttggcg
    14461 aactgggaca ctgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga
    14521 gccgtaattt gttagcgatt gttcacccta tcctgcgcaa tctaatggaa gagtctggcg
    14581 aaacggtcaa tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgatcagg
    14641 tacagtgtac tcacctgatg cgtatgtccg cacctatcgg cggtaaattg ccgatgcacg
    14701 cttccggtgc aggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc
    14761 tgcaccgcaa agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa
    14821 aagaagatct cgcccaaacg cgcaaacggg gctattcatt tgacgatgag gaacatgcac
    14881 tggggctacg ttgccttgca gcgtgtattt tcgatgagca ccgtgaaccg tttgccgcaa
    14941 tttctatttc cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga
    15001 tggtgattaa agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc
    15061 tggcgggcag aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct
    15121 gttaatcgca ttgacgccaa aataacttaa tgtcatacac ttcacacact tttattaact
    15181 cagcattatt tttaaacatc aaaccactta attattaaca aaatcgcaag atctcattcc
    15241 accgaaactt ttacggctgc attatgagat tgagcgattt atgatatttc tttagttgta
    15301 atcatattaa attttttcac tgttctgata gttaaaattt aagacatcaa taaacaatga
    15361 gatatttaaa tgattactcg tattcctcgt agttctttct ctgcaaatat taataataca
    15421 gcccagacaa atgaacacca aaccctgagt gaattgtttt ataaagaact cgaggataaa
    15481 ttttctggca aggagctggc gacgcctcta ttaaaaagct tctcagagaa ctgtagacaa
    15541 aatggtcgtc atatctttag caacaaggat tttg
//
LOCUS       DAZOZY010000088        15511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZOZY010000088 DAZOZY010000000
VERSION     DAZOZY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.088"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHURQ_002382"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHURQ_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR0824134.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHURQ_002383"
     CDS             567..650
                     /locus_tag="ACHURQ_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824135.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHURQ_002384"
     CDS             750..1571
                     /locus_tag="ACHURQ_002384"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR0824136.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHURQ_002385"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHURQ_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR0824137.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHURQ_002386"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHURQ_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR0824138.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHURQ_002387"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHURQ_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR0824139.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHURQ_002388"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHURQ_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR0824140.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPAAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHURQ_002389"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHURQ_002389"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR0824141.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHURQ_002390"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHURQ_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR0824142.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHURQ_002391"
     CDS             8347..9729
                     /locus_tag="ACHURQ_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0824143.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHURQ_002392"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHURQ_002392"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR0824144.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHURQ_002393"
     CDS             complement(10485..10820)
                     /locus_tag="ACHURQ_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR0824145.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHURQ_002394"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHURQ_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR0824146.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHURQ_002395"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHURQ_002395"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR0824147.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHURQ_002396"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHURQ_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR0824148.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHURQ_002397"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHURQ_002397"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR0824149.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEQGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3811 a         4021 c         3793 g         3886 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcggcaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc ctgctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZOZY010000089        15444 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZOZY010000089 DAZOZY010000000
VERSION     DAZOZY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.089"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHURQ_002398"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="ACHURQ_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR0824150.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="ACHURQ_002399"
     CDS             1598..4654
                     /locus_tag="ACHURQ_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR0824151.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="ACHURQ_002400"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="ACHURQ_002400"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR0824152.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="ACHURQ_002401"
     CDS             5161..5349
                     /locus_tag="ACHURQ_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR0824153.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="ACHURQ_002402"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="ACHURQ_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR0824154.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="ACHURQ_002403"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="ACHURQ_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR0824155.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="ACHURQ_002404"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="ACHURQ_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR0824156.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="ACHURQ_002405"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="ACHURQ_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR0824157.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="ACHURQ_002406"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="ACHURQ_002406"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR0824158.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="ACHURQ_002407"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="ACHURQ_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR0824159.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHURQ_002408"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="ACHURQ_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR0824160.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHURQ_002409"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="ACHURQ_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR0824161.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHURQ_002410"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="ACHURQ_002410"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR0824162.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(14591..14785)
                     /locus_tag="ACHURQ_002411"
     CDS             complement(14591..14785)
                     /locus_tag="ACHURQ_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824163.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="ACHURQ_002412"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="ACHURQ_002412"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR0824164.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3901 a         3738 c         4089 g         3716 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gacagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acga
//
LOCUS       DAZOZY010000090        15406 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZOZY010000090 DAZOZY010000000
VERSION     DAZOZY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.090"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..453
                     /locus_tag="ACHURQ_002413"
     CDS             <1..453
                     /locus_tag="ACHURQ_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0824165.1"
                     /translation="GLCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRIS
                     LPTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPP
                     PFHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="ACHURQ_002414"
                     /pseudo
     CDS             669..758
                     /locus_tag="ACHURQ_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHURQ_002415"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="ACHURQ_002415"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR0824166.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHURQ_002416"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="ACHURQ_002416"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR0824167.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHURQ_002417"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="ACHURQ_002417"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0824168.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHURQ_002418"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="ACHURQ_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR0824169.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="ACHURQ_002419"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="ACHURQ_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR0824170.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="ACHURQ_002420"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="ACHURQ_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR0824171.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="ACHURQ_002421"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="ACHURQ_002421"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR0824172.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="ACHURQ_002422"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="ACHURQ_002422"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR0824173.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="ACHURQ_002423"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="ACHURQ_002423"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR0824174.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="ACHURQ_002424"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="ACHURQ_002424"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR0824175.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHURQ_002425"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="ACHURQ_002425"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR0824176.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHURQ_002426"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="ACHURQ_002426"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR0824177.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="ACHURQ_002427"
     CDS             13055..13435
                     /locus_tag="ACHURQ_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR0824178.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="ACHURQ_002428"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="ACHURQ_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR0824179.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHURQ_002429"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="ACHURQ_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR0824180.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /locus_tag="ACHURQ_002430"
     CDS             complement(15244..>15406)
                     /locus_tag="ACHURQ_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0824181.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4042 c         3851 g         3597 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt tagaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaata taatggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaga
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAZOZY010000091        15259 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZOZY010000091 DAZOZY010000000
VERSION     DAZOZY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15259
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.091"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            87..956
                     /locus_tag="ACHURQ_002431"
     CDS             87..956
                     /locus_tag="ACHURQ_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR0824182.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1159..1512)
                     /locus_tag="ACHURQ_002432"
     CDS             complement(1159..1512)
                     /locus_tag="ACHURQ_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR0824183.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1650..3296)
                     /gene="groL"
                     /locus_tag="ACHURQ_002433"
     CDS             complement(1650..3296)
                     /gene="groL"
                     /locus_tag="ACHURQ_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR0824184.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3340..3633)
                     /locus_tag="ACHURQ_002434"
     CDS             complement(3340..3633)
                     /locus_tag="ACHURQ_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR0824185.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3909..5165
                     /gene="yjeH"
                     /locus_tag="ACHURQ_002435"
     CDS             3909..5165
                     /gene="yjeH"
                     /locus_tag="ACHURQ_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR0824186.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5187..5657)
                     /locus_tag="ACHURQ_002436"
     CDS             complement(5187..5657)
                     /locus_tag="ACHURQ_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR0824187.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5994..7430
                     /gene="aspA"
                     /locus_tag="ACHURQ_002437"
     CDS             5994..7430
                     /gene="aspA"
                     /locus_tag="ACHURQ_002437"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR0824188.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7548..8849
                     /gene="dcuA"
                     /locus_tag="ACHURQ_002438"
     CDS             7548..8849
                     /gene="dcuA"
                     /locus_tag="ACHURQ_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR0824189.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8965..9303
                     /gene="cutA"
                     /locus_tag="ACHURQ_002439"
     CDS             8965..9303
                     /gene="cutA"
                     /locus_tag="ACHURQ_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR0824190.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9279..10976
                     /gene="dsbD"
                     /locus_tag="ACHURQ_002440"
     CDS             9279..10976
                     /gene="dsbD"
                     /locus_tag="ACHURQ_002440"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR0824191.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11013..11588
                     /locus_tag="ACHURQ_002441"
     CDS             11013..11588
                     /locus_tag="ACHURQ_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0824192.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11695..11770
                     /locus_tag="ACHURQ_002442"
     tRNA            11695..11770
                     /locus_tag="ACHURQ_002442"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11728..11730,aa:Phe,seq:gaa)
     gene            12013..12127
                     /locus_tag="ACHURQ_002443"
                     /pseudo
     CDS             12013..12127
                     /locus_tag="ACHURQ_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            12387..12686
                     /locus_tag="ACHURQ_002444"
     CDS             12387..12686
                     /locus_tag="ACHURQ_002444"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR0824193.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(12677..12802)
                     /gene="laoB"
                     /locus_tag="ACHURQ_002445"
     CDS             complement(12677..12802)
                     /gene="laoB"
                     /locus_tag="ACHURQ_002445"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR0824194.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            12869..13924
                     /gene="cadC"
                     /locus_tag="ACHURQ_002446"
     CDS             12869..13924
                     /gene="cadC"
                     /locus_tag="ACHURQ_002446"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR0824195.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
     gene            14289..>15259
                     /gene="cadB"
                     /locus_tag="ACHURQ_002447"
     CDS             14289..>15259
                     /gene="cadB"
                     /locus_tag="ACHURQ_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR0824196.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3768 a         3792 c         3649 g         4050 t
ORIGIN      
        1 ttaacaacta ttactaccaa gcccatttat ctggcttata aattaaaatt aattatttaa
       61 ttgagaaatg gttagggaga acctacatgg ccataagcat caagggtgtt aataccggtg
      121 ttattcgcaa gagtaataat ttcattgcac tggcgctgaa aattaaagaa cctcgcaata
      181 aagaatcgtt gttctttatg tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa
      241 gtcgtctgca tcaaaagcat aaactgaatg ctgctgcacg ccttcagtat gagcaagctc
      301 gcgataaagt cattaagaaa atggcagaaa acatcccaga aattctggtt gatgagctta
      361 aaaatgccga tattaatcgt cgggttaaca cgctggaact taccgataat caaggcgaaa
      421 atcttacctt cgtattaacg ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt
      481 tgcagattga aatgctggcg cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg
      541 aactggcatt acgtattacc tcactgttag atttcctgcc gttatacgac gttgactgcc
      601 aggacaatgg caatctggaa tatgacacct attcccaacc ggaatggaaa cataacctgt
      661 tcgaccatta tctggcggtg ctttaccgct ttaaagatga aagtggcaaa gaacaattta
      721 gcggcacagt ggtgaaaacg cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc
      781 gccgaatgct tgatttcagc ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct
      841 atgttcgcac cgtagcggcg aataacgccc aaccgctaac ccaggatcaa tgcttacgcg
      901 cgctgcatca cttacgtgtt caatccacca gcaaaactgc accacaggcg aaataatctc
      961 atctttgcta atgcgaggct ggagccatcc agcctgaaat gactgataac gtgatcgttg
     1021 gtcatgccgg atgcgacgta aacgccttat ccggcctaca aattctcaag cattcaatca
     1081 gttgcaatgc ctgctgtagg cctgataagc gaagcgcatc aggcaataat acgtttgtca
     1141 cccagcctct ggatcacatc agttcgggca cttataaacc tgaccgataa tctggctatc
     1201 cgtcgggaca aaactggaca acattccctg cgacgggctg ctgatgccat aaatcacgtt
     1261 accgcccatt gcagccgcct ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc
     1321 ctcttctccg tgttgcccgg aaagccagtt gctttgctta cctgtcgcag taccaatcag
     1381 ctggcactct gcgcctggct gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa
     1441 ttcgttgctg gagctacaac ccgccatcag tagcgcggca ccgacaatcc ctgctaagta
     1501 ttttacgtgc atgttattcc ccataatcaa tgagctggac gccactcgcg tcgtccgtgt
     1561 ctgaatctta tactagaaag atgaccaaaa gaaaaacccc cagacatttc tgcccggggg
     1621 tttgtttatt tctgcgaggt gcagggcaat tacatcatgc cgcccatgcc acccatgccg
     1681 cccataccgc cagcagcgcc taagtcagct gcatcatttt tcggcaggtc ggtaaccatg
     1741 cactcggtgg tgatcatcag gccagccaca gaagccgcgt actgcagagc agaacgggtt
     1801 actttggttg ggtccaggat acccatgtcg atcatgttgc cgtattcttc ggttgctgcg
     1861 ttgtaaccgt agttgccgtc gccgccttta acggtgttag caacaacaga cggttcttcg
     1921 ccgcagttca ggacgatctg acgcagcgga gcttccattg cacgcagtgc aactttgata
     1981 cccacgttct ggtcttcgtt ctgaccacgc aggtcagcca gtttagacgc tacgcggatc
     2041 agcgcaacac caccaccagc aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg
     2101 gcgtcttcaa cgcgtgcttt tttctctttc atttcaactt cggtagcagc acctactttg
     2161 ataactgcaa cgccgcctgc cagtttcgct acgcgttcct gcagtttttc acggtcgtag
     2221 tcagaagttg cttcttcaat ctgctgacgg atctgagcaa cacggccctg gattgcagct
     2281 tcttcaccca cgccatcgat gatagtggtg gtgtctttgt tgatcacaac acgtttagcc
     2341 tgacccaggt cttccagggt tgctttttcc agttccatac cgatctcttc agagatcacg
     2401 gtaccgccag tcagggttgc gatatcctgc agcatagctt tacgacgatc gccgaagccc
     2461 ggagctttaa ctgcagcaac tttcacgatg ccacgcatgg tgttaacaac cagagttgcc
     2521 agcgcttcgc cttctacatc ttcagcgatg atcagcagcg gtttgcctgc tttggcaacg
     2581 gcttccagaa ccggcagcat ttcgcggatg ttggagattt tcttgtcagc cagcaggatg
     2641 aacgggcttt ccagttctac tgcgccagtt tccggcttgt tgatgaagta aggagacagg
     2701 tagccacggt cgaactgcat accttcaacc acgtccagtt cgtcctgcag accggtaccg
     2761 tcttcaacgg tgataacgcc ttctttaccg actttgtcca tcgcttcagc gatcagttta
     2821 cctacggttt cgtcggagtt agcggagatg gtaccaacct gagcaatcgc tttagagtca
     2881 gagcacggta cggacagcgc tttcagttct tcaactgcag cggtaacggc tttgtcgata
     2941 ccacgtttca ggtccatcgg gttcatgccc gcagcaacag ctttcagacc ctcagtgatg
     3001 atagcctgag ccagtacagt tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgcttta
     3061 gaggcaactt ctttcaccat ctgcgcaccc atgttttcga acttgtcttc cagttcgatt
     3121 tcacgagcaa cggaaacacc atctttggtg atggtcggtg caccgaaaga tttatccaga
     3181 actacgttac ggcctttcgg accgagggta actttcactg catctgccag tacgtttacg
     3241 ccgcgcagca ttttcacacg agcgtcgtta ccgaatttta cgtctttagc tgccattatc
     3301 tttattcctt aaattcgtat gttcagtgtc gtgcgaggat tacgcttcaa caattgccag
     3361 aatgtcgctt tcggacatga tcaacacttc ttcattgtcg atcttctcag atttcacacc
     3421 gtagccatcg ttgaaaataa cgatgtcgcc aactttcaca tccagcggct tcacttcgcc
     3481 attttcaagg atacggccat tgccgacagc cagcacttcg ccgcgggtgg atttagccgc
     3541 tgcagagccg gtcagaacga tgccgccagc agatttagtt tcaacttctt tacgcttgac
     3601 gatcacgcga tcatgcaatg gacgaatatt cattgataac tctcctttga gaaagtccgt
     3661 atctgttatg ggtgacgccg gagcttacgt ggtttcccgg ctggtgacca gagaaatggg
     3721 gatgaggatt cgccccttca agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg
     3781 ggtgaaaacc ctgattcacc tcacatttca tcgcaatttc ttcatcgccg tcataagcga
     3841 atctgattgt gctaccatcg aaaatctacg cagttgccca aaatttgggc gcaatcggat
     3901 atcaacccat gagtggactc aaacaagaac tggggctggc ccagggcatt ggcctgctat
     3961 cgacgtcatt attaggcacc ggcgtgtttg ccgttcctgc gttagctgca caggtagcag
     4021 gcaataacag cctgtgggcg tggcccgttt tgattatctt agtgttcccg attgcgattg
     4081 tgtttgcgat tctgggtcgc cactatccca gcgcaggcgg tgtcgcgcac ttcgtcggta
     4141 tggcgtttgg ttcgcggctt gagcgagtca ccggctggtt gtttttatcg gtcattcccg
     4201 tgggtttgcc tgccgcgcta caaattgccg ccgggttcgg ccaggcaatg tttggctggc
     4261 atagctggca actgttgttg gcagaactcg gtacgctggc gctggtgtgg tatatcggta
     4321 ctcgcggtgc cagttccagt gctaatctac aaaccgttat tgccggactt atcgtcgcgc
     4381 tgattgtcgc tatctggtgg gcgggcgata tcaaacctgc gaatatcccc ttcccggcac
     4441 ctggtaatat cgaacttacc gggttatttg ctgcgttatc agtgatgttc tggtgttttg
     4501 tcggtctgga agcatttgcc catcttgcct cggaatttaa aaatccagag cgtgattttc
     4561 ctcgcgcatt gatgattggt ctgctgctgg caggattagt ctactggggc tgtacggtag
     4621 tcgtcttaca cttcgacgcc tatggtgaac aaatggccgc ggcagcatcg cttccaaaaa
     4681 ttgtagtgca actgttcggt gtaggagcgt tatggattgc ctgcgtaatt ggctatctga
     4741 cctgctttgc cagtctcaac atttatatac agagcttcgc ccgcctggtc tggtcgcagg
     4801 cgcaacataa tcctgaccac tacctggcac gcctctcttc tcgccatatc ccgaataatg
     4861 ccctcaatgc ggtgctcggc tgctgcgtgg tgagcacttt ggtgattcat gctttagaga
     4921 tcaatctgga cgctcttatt atttatgcca atggcatctt tattatgatt tatctgttat
     4981 gcatgctggc aggctgtaaa ttattgcaag gacgttatcg actactggcg gtggttggcg
     5041 ggctgttatg cgttctgtta ctggcaatgg tcggctggaa aagtctctat gcgctgatta
     5101 tgctggcggg gttatggctg tttctgccaa aacgaaaaac gccggaaaat ggcataacca
     5161 cataatccgg cgtttcgaca ttaatcctag cgatcgtctt tatgatcaag gcggtcgcgg
     5221 tcatcatcct ttcgctggta ctcaccatca aaagtattac cgccaccggt cccggcgcta
     5281 aaaccgccgc ctggcatgcg agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc
     5341 ttttgcaccg gcggcaataa aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat
     5401 aaaagcagca aaccggcaat aatcagcgaa acacttttaa tcatctccgc cgcagggttt
     5461 tcacccgccg ccattttttg ctgcatcagc acgaaattct taaagccctg gttacgtacc
     5521 agtgacatac cgataactga cgtgaatata accagcacga gggtcagcaa tacccccaat
     5581 acatgggcaa cctgaataaa gattgaaatc tcaatataga cataaaggaa aatggcaata
     5641 aaaggtaacc agcgcaaagg tttctcctgt aatagcagcc ggttaaaccc ggctacctga
     5701 atgggttgcg aatcgcgttc agcttatatt gtggtcatta gcaaaatttc aagatgtttg
     5761 cgcaactatt tttggtagta atcccaaagc ggtgatctat ttcacaaatt aataattaag
     5821 gggtaaaaac cgacacttaa agtgatccag attacggtag aaatcctcaa gcagcatatg
     5881 atctcgggta ttcggtcgat gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca
     5941 tatattctgt gtgtttaaag caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa
     6001 acaacattcg tatcgaagaa gatctgttgg gtaccaggga agttccagct gatgcctact
     6061 atggtgttca cactctgaga gcgattgaaa acttctatat cagcaacaac aaaatcagtg
     6121 atattcctga atttgttcgc ggtatggtaa tggttaaaaa agccgcagct atggcaaaca
     6181 aagagctgca aaccattcct aaaagtgtag cgaatgccat cattgccgca tgtgatgaag
     6241 tcctgaacaa cggaaaatgc atggatcagt tcccggtaga cgtctaccag ggcggcgcag
     6301 gtacttccgt aaacatgaac accaacgaag tgctggccaa tatcggtctg gaactgatgg
     6361 gtcaccagaa aggtgaatat cagtacctga acccgaacga ccatgttaac aaatgtcagt
     6421 ccactaacga cgcctacccg accggtttcc gtatcgcagt ttactcttcc ctgattaagc
     6481 tggtagatgc gattaaccaa ctgcgtgaag gctttgaacg taaagctgtc gaattccagg
     6541 acatcctgaa aatgggtcgt acccagctgc aggacgcagt accgatgacc ctcggtcagg
     6601 aattccgcgc tttcagcatc ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg
     6661 aactgctgct ggaagttaac cttggcgcaa cagcaatcgg tactggtctg aacacgccga
     6721 aagagtactc tccgctggca gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac
     6781 cggctgaaga cctgatcgaa gcgacctctg actgcggcgc ttatgttatg gttcacggcg
     6841 cgctgaaacg cctggctgtg aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt
     6901 ctggtccacg tgccggcctg aacgagatca acctgccgga actgcaggcg ggctcttcca
     6961 tcatgccagc taaagtaaac ccggttgttc cggaagtggt taaccaggta tgcttcaaag
     7021 tcatcggtaa cgacaccact gttaccatgg cagcagaagc aggtcagctg cagttgaacg
     7081 ttatggagcc ggtcattggc caggctatgt tcgaatccgt tcacattctg accaacgctt
     7141 gctacaacct gctggaaaaa tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag
     7201 gttacgttta caactctatc ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca
     7261 acggtgacat cgtgggtaaa atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc
     7321 tggaacgcgg tctgttgact gaagcggaac ttgacgatat tttctccgta cagaatctga
     7381 tgcacccggc ttacaaagca aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg
     7441 tagtacaaat aaagaaggca cgtcagatga cgtgcctttt ttcttgtgag cagtaactta
     7501 aaaataacaa tctaatatca acttgttaaa aaacaaggaa ggctaatatg ctagttgtag
     7561 aactcatcat agttttgctg gcgatcttct tgggcgccag attgggggga ataggtattg
     7621 gttttgcagg cggattgggg gtgctggttc ttgccgctat tggcgttaaa cccggtaaca
     7681 tcccgttcga tgtcatttcc attatcatgg cggttatcgc cgctatttct gccatgcagg
     7741 ttgctggcgg tctggactat ctggttcatc agacagaaaa gctgctgcgc cgtaacccga
     7801 aatacatcac aatcctcgca ccgatcgtga cctatttcct gacaatcttt gctggtactg
     7861 gcaacatctc tctggcgaca ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta
     7921 aaccttgccg tccgctgtct actgcagtgg tatccgcgca gattgcgatc accgcatcgc
     7981 caatctcagc ggcagtggtt tacatgtctt ccgtgatgga aggtcatggc atcagctacc
     8041 tccatctgct ctccgtggtc atcccgtcca ccctgctggc ggttctggtg atgtccttcc
     8101 tggtcactat gctgttcaac tccaaactct ctgacgatcc gatttatcgc aagcgtctgg
     8161 aagagggcct ggttgaactg cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa
     8221 cgtccgtctg gctgttcctg ctgggcgtag ttggcgtggt tatctatgca atcatcaaca
     8281 gcccaagcat gggtctggtt gaaaaaccgc tgatgaacac caccaacgca atcctgatca
     8341 tcatgctcag cgttgcaact ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc
     8401 tcaactccag caccttcaaa gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt
     8461 ggctgggcga tactttcgtt tccaacaaca tcgactggat caaagatacc gctggtgaag
     8521 tgattcaggg tcatccgtgg ctgctggccg tcatcttctt ctttgcttct gctctgctgt
     8581 actctcaggc tgcaaccgca aaagcactga tgccgatggc tctggcactg aacgtttctc
     8641 cgctgaccgc tgttgcttct ttcgctgcgg tgtctggtct gttcattctg ccgacctatc
     8701 cgacgctggt tgctgcggta cagatggatg acacgggtac aacccgtatc ggtaaattcg
     8761 tcttcaacca tccgttcttc atcccgggta ctctgggtgt tgccctggcc gtttgcttcg
     8821 gcttcgtgct gggtagcttc atgctgtaat gaccaatcgc ggggcgttca cgccccgctt
     8881 tctttcccgc cgactaacat ccttcccccg tccgttgtat agtgacctct ctcttgcggt
     8941 tccatctgtt cttgcgaggt gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc
     9001 gtggtgctat gtaccgcacc ggatgaagcg acagcccagg atttagccgc caaagtgctg
     9061 gcggaaaaac tggcggcctg cgcgaccttg atccccggcg ctacctctct ctattactgg
     9121 gaaggtaagc tggagcaaga atacgaagtg cagatgattt taaaaactac cgtatctcac
     9181 cagcaggcac tgctggaatg cctgaagtct catcatccat atcaaacccc ggaacttctg
     9241 gttttacctg ttacacacgg agacacagat tacctctcat ggctcaacgc atctttacgc
     9301 tgatcctgct actttgcagc acttccgttt ttgccggatt attcgacgcg ccgggacgtt
     9361 cacattttgt ccccgcggat caagcctttg cttttgattt tcagcaaaac caacatgacc
     9421 tgaatctgac ctggcagatc aaagacggtt actacctcta ccgtaaacag atccgcatta
     9481 cgccggaaca cgcgaaaatt gccgacgtgc agctgccgca aggcgtctgg catgaagatg
     9541 agttttacgg caaaagcgag atttaccgcg atcggctgac gcttcccgta accatcaacc
     9601 aggcgagtgc gggagcgacg ttaactgtca cctaccaggg ctgtgctgat gccggtttct
     9661 gttatccgcc agaaaccaaa accgttccgt taagcgaagt ggtcgccaac aacgcagcgt
     9721 cacagcctgt gtctgttccg cagcaagagc agcacaccgc gcaattgccc ttttccgcgc
     9781 tctgggcgtt gttgatcggt attggtatcg cctttacgcc atgcgtgctg ccaatgtacc
     9841 cactgatttc tggcatcgtg ctgggtggta aacagcggct ctccactgcc agagcattgt
     9901 tgctgacctt tatttatgtg caggggatgg cactgactta cacggcgctg ggtctggtag
     9961 ttgccgccgc agggttacag ttccaggcgg cgctacagca cccatacgtg ctcattggcc
    10021 tcgccatcgt ctttaccttg ctggcgatgt caatgtttgg cttgtttact ctacaactcc
    10081 cctcttcgct gcaaacacat ctcacattga tgagcaatcg ccaacagggc ggctcacctg
    10141 gcggtgtgtt tgttatgggg gcgattgccg gactgatctg ttcaccatgc accaccgcac
    10201 cgcttagcgc gattctgctg tatatcgccc aaagcgggaa catgtggctg ggcggcggca
    10261 tgctttatct ctatgcgttg ggcatgggcc tgccgctgat gctaattacc gtctttggta
    10321 accgcttgct gccgaaaagc ggcccgtgga tggaacaagt caaaaccgcg tttggttttg
    10381 tgatcctcgc actgccgatc ttcctgctgg agcgagtgat tggtgatata tggggattac
    10441 gcttgtggtc ggcgctgggt gtcgcattct ttggctgggc ctttatcacc agcctacagg
    10501 ccaaacgcgg ctggatgcgc gtggtgcaaa taatcctgct ggcagcggca ttggttagcg
    10561 tgcgcccact tcaggattgg gcatttggtg cgacgcatac cgcgcaaact cagacgcatc
    10621 tcaactttac acaaatcaaa acggtagatg agttaaatca ggcgctcgtt gaagccaaag
    10681 gcaaaccggt gatgttagat ctttatgccg actggtgcgt cgcctgtaaa aagtttgaga
    10741 aatacacctt cagcgacccg caggtgcaaa aagcgttagc agacacggtc ttacttcagg
    10801 ccaacgtcac tgctaacgac gcacaagatg tggcgctgtt aaagcatctt aatgtccttg
    10861 gactaccgac aattctcttt tttgacggac aaggccagga gcatccacaa gcacgcgtca
    10921 cgggctttat ggatgctgaa accttcagcg cacatttgcg cgatcgccaa ccgtgaacga
    10981 cacttgcagt gggaaagacg gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag
    11041 ccctgaaatt attagaatta caagggattg ccaacaccac gctggagatg gttgctgaac
    11101 gtgtggatta tccactggac gagctacgcc gcttctggcc agacaaagag gcaatcctct
    11161 acgatgcgct gcgttatctt agccaacaga tagatgtctg gcgtcgtcag ctgatgcttg
    11221 acgaaacgca aaccgccgaa caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg
    11281 ttaaaaacaa ccgctatccg ggctgtctat ttatcgctgc ctgtacgttt tatcccgacc
    11341 ctggccaccc tattcatcaa ctggccgatc agcaaaaaag cgcggcctac gatttcaccc
    11401 acgaactgtt aaccacgctg gaagttgacg atccggcgat ggtagcaaag cagatggaac
    11461 tggtgctgga aggctgttta agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca
    11521 ccgcacatcg gctggcggaa gatatcctgc gcttcgcccg ctgccgccag ggtggtgcac
    11581 tgacctgaca gaaacacaga aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct
    11641 tttactgaaa ttaggttgac gagatgtgca gattacggtt taatgcgccc cgttgcccgg
    11701 atagctcagt cggtagagca ggggattgaa aatccccgtg tccttggttc gattccgagt
    11761 ccgggcacca aattcatata aacggacctc cacggaggtc cgtttttcgt ttcaggacgc
    11821 cacgatttaa gcgttctgcc tccaaatcaa ttctaccgaa ctcaaccaga ttctccccac
    11881 atcaccagca ttttgcgggt atatcccaat tcgggaaaat ttgttcctga gcgatagcac
    11941 tgactgacgt gaaatgtcgt gcggccccgt gatgttgttg aacgtcaaat gacgtcatca
    12001 ggagcgtaac gcatccataa agcacaacac cggtctgaac ggcgactgat gagattttct
    12061 gaatgagaac aaagagaaat gtatcagtcc gcttgctcat gcaaagacta acaatccatt
    12121 aaaatagtaa gcgctccgga caattttcca tggattattt tctacacatt tttctttggc
    12181 aaagatgatg aattttgatg gtaaggaata ttacttctgg ttctcagtaa aatcctttcg
    12241 taatactatg taatcaagaa gtttatggct ggtaaaaata acgtcttgca ttcaccaata
    12301 acatgtaaat aaacccatct attgatggta aaaataggtc atggcaatta tcattgcacc
    12361 attccctttt cgaatgagtt tctattatgc aacaacctgt agttcgcgtt ggcgaatggc
    12421 ttgttactcc gtccataaac caaattagcc gcaatgggcg tcaacttacc cttgagccga
    12481 gattaattga tcttctggtt ttctttgctc aacacagtgg cgaagtactt agcagggatg
    12541 aacttatcga taatgtctgg aagagaagta ttgtcaccaa tcacgttgtg acgcagagta
    12601 tctcagaact acgtaagtca ttaaaagata atgatgaaga tagtcctgtc tatatcgcta
    12661 ctgtaccaaa gcgcggctat aaatgaatgg tgccggttat ctggtacagc gaagaagagg
    12721 gagaggaaat aatgctatct tcgcctcccc ctataccaga ggcggttcct gacacaaatt
    12781 ctccctccca cagtcttacc attcaaaaca ccgcaacgcc acctgaacaa tccccagtta
    12841 aaagcaaacg attcactacc ttttgggtat ggtttttttc ctgttgtcgt taggtatctg
    12901 tgtagcactg gtagcgtttt caactcttga tacacgtctt cctatgagca aatcgcgtat
    12961 tttgctcaat ccacgcgata ttgacattaa tatggtaaat aaaagttgta acagctggag
    13021 ttccccgtat cagctctctt acgcgatagg cgtgggtgat ttggtggcga catcacttaa
    13081 caccttctcc acctttatgg tgcatgacaa aatcaactac aacattgatg aaccgagcag
    13141 ttccggtaaa acattatcta ttgcctttgt taatcagcgc caataccgtg ctcaacaatg
    13201 ctttatgtcg gtaaaattag tagacaatgc agatggttca accatgctgg ataaacgtta
    13261 tgtcatcact aacggtaatc agctggcgat tcaaaatgat ttactggaga gtttatcaaa
    13321 agcgttaaac caaccgtggc cacaacgaat gcaggagacg ctccagcaaa ttttgcctca
    13381 tcgtggtgcg ttattaacta atttttatca ggctcatgat tatttactgc atggcgatga
    13441 caaatcattg aatcgtgcca gtgaattatt aggtgagatt gttcaatcat ccccagaatt
    13501 tacctacgcg agagcagaaa aagcattagt tgatatcgtg cgccattctc aacacccttt
    13561 agatgaaaaa caattagcag cactgaacac agaaatagac aacattgtta cactgccgga
    13621 attgaacaac ctgtccatta tatatcaaat aaaagcggtc agtgctctgg taaaaggtaa
    13681 aatagatgag tcttaccagg cgataaatac tggcattgat cttgaaatgt cctggctaaa
    13741 ttatgtgttg cttggcaagg tttatgaaat gaaggggatg aaccgggaag cagctgatgc
    13801 atatctcact gcctttaatt tacgcccagg ggcaaacacc ctttactgga ttgaaaatgg
    13861 tattttccag acttctgttc cttatgttgt accttatctc gacaaatttc tcgcttcaga
    13921 ataagtaact ccgggttgat ttatgctctg gaatatttgt tgttgagttt ttgtatgttc
    13981 ccattggtat aatatgttgc ggcaatttat ttgccgcata atttttatta cataaattta
    14041 accagagaat gtcatgcaat ccattgtaaa cattgaatgt ttatcttttc atgatatcaa
    14101 cttgcgatcc tgatgtgtta ataaaaaacc tcaagttctc acttacagaa acttttgtgt
    14161 tatttcacct aatctttagg attaatcctt ttttcgtgag taatcttatc gccagtttgg
    14221 tctggtcagg aaatagttat acatcatgac ccggactcca aattcaaaaa tgaaattagg
    14281 agaagagcat gagttctgcc aagaagatcg ggctatttgc ctgtaccggt gttgttgccg
    14341 gtaatatgat ggggagcggt attgcattat tacctgcgaa cctagcaagt atcggtggta
    14401 ttgctatctg gggttggatt atctctatta ttggtgcaat gtcgctggcg tatgtatatg
    14461 cccgactggc aacaaaaaac ccgcaacaag gtggcccaat tgcttatgcc ggagaaattt
    14521 ctcctgcatt tggttttcag acaggtgttc tttattacca tgctaactgg attggtaacc
    14581 tagcgattgg tattaccgct gtatcttatc tttccacctt cttcccagta ttaaatgatc
    14641 ctgttccggc gggtatcgcc tgtattgcta tcgtctgggt atttaccttt gtaaatatgc
    14701 tcggcggtac ctgggtaagc cgtttaacca ctattggtct ggtgctggtt cttattcctg
    14761 tggtgatgac tgctattgtt ggctggcatt ggtttgatgc ggcaacttat gcagctaact
    14821 ggaatactgc ggataccact gatggtcatg cgatcattaa aagtattctg ctctacctgt
    14881 gggccttcgt gggtgttgag tccgcagcag taagtactgg tatggttaaa aacccgaaac
    14941 gtaccgttcc gctggcaacc atgctgggta ctggtttagc aggtattgtt tacatcgctg
    15001 cgactcaggt gctttccggt atgtatccgt cttctgtaat ggcggcttcc ggtgctccgt
    15061 ttgcaatcag tgcttcaact atcctcggta actgggctgc gccgctggtt tctgcattca
    15121 ccgcctttgc gtgcctgact tctctgggct cctggatgat gttggtaggc caggcaggtg
    15181 tacgtgccgc taacgacggt aacttcccga aagtttatgg tgaagtcgac agcaacggta
    15241 ttccgaaaaa aggtctgct
//
LOCUS       DAZOZY010000092        15231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZOZY010000092 DAZOZY010000000
VERSION     DAZOZY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.092"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..350
                     /locus_tag="ACHURQ_002448"
     CDS             <1..350
                     /locus_tag="ACHURQ_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0824197.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
     gene            complement(359..1666)
                     /gene="ynjE"
                     /locus_tag="ACHURQ_002449"
     CDS             complement(359..1666)
                     /gene="ynjE"
                     /locus_tag="ACHURQ_002449"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR0824198.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(1733..2386)
                     /locus_tag="ACHURQ_002450"
     CDS             complement(1733..2386)
                     /locus_tag="ACHURQ_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR0824199.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(2386..3922)
                     /locus_tag="ACHURQ_002451"
                     /pseudo
     CDS             complement(2386..3922)
                     /locus_tag="ACHURQ_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            complement(3895..5061)
                     /locus_tag="ACHURQ_002452"
     CDS             complement(3895..5061)
                     /locus_tag="ACHURQ_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR0824200.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(5071..5619)
                     /locus_tag="ACHURQ_002453"
     CDS             complement(5071..5619)
                     /locus_tag="ACHURQ_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR0824201.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(5619..6326)
                     /locus_tag="ACHURQ_002454"
     CDS             complement(5619..6326)
                     /locus_tag="ACHURQ_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0824202.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(6341..7018)
                     /gene="ydjY"
                     /locus_tag="ACHURQ_002455"
     CDS             complement(6341..7018)
                     /gene="ydjY"
                     /locus_tag="ACHURQ_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR0824203.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(7023..7733)
                     /locus_tag="ACHURQ_002456"
     CDS             complement(7023..7733)
                     /locus_tag="ACHURQ_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR0824204.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(7900..8706)
                     /gene="xthA"
                     /locus_tag="ACHURQ_002457"
     CDS             complement(7900..8706)
                     /gene="xthA"
                     /locus_tag="ACHURQ_002457"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR0824205.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            9152..10372
                     /gene="astC"
                     /locus_tag="ACHURQ_002458"
     CDS             9152..10372
                     /gene="astC"
                     /locus_tag="ACHURQ_002458"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR0824206.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            10369..11403
                     /gene="astA"
                     /locus_tag="ACHURQ_002459"
     CDS             10369..11403
                     /gene="astA"
                     /locus_tag="ACHURQ_002459"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR0824207.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            11400..12878
                     /gene="astD"
                     /locus_tag="ACHURQ_002460"
                     /pseudo
     CDS             11400..12878
                     /gene="astD"
                     /locus_tag="ACHURQ_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            12875..14218
                     /gene="astB"
                     /locus_tag="ACHURQ_002461"
     CDS             12875..14218
                     /gene="astB"
                     /locus_tag="ACHURQ_002461"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR0824208.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            14211..15179
                     /gene="astE"
                     /locus_tag="ACHURQ_002462"
     CDS             14211..15179
                     /gene="astE"
                     /locus_tag="ACHURQ_002462"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR0824209.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         3527 a         3985 c         4196 g         3523 t
ORIGIN      
        1 tcttttacgc gctggtgccg tttggcttta ttcttgctgc accggagcaa aatgcgctgg
       61 caggcggctg gttgttgttt gcgtttatcg gcacgggcag cagttttctc gcctttgctg
      121 cactggcagc aaaacatcag attgataacc ccggttatgc acataagtcg ttttactatc
      181 tggggggatt aaccgaaggc acagaaacga tcttactgtt tgtgctggga tgtttattcc
      241 ctgcatggtt tgcgtggttt gcgtggatct tcggtgcgtt gtgctggatg acgaccttta
      301 cgcgggtgtg gagtgggtat ctgacgctga agtcgctcca gcgtcagtaa ttgcaatgtt
      361 atttgctact gtccgggccg cgttcaccgg ttgctaccgg attttttgga tcgctgctcc
      421 attcgtacca gccgccgtca tacacggaaa cattcttcca acccatggcg cgtgcgtaca
      481 taaaggtttc ggacgcgcgc cagccggtgc cgcagtagaa tgaaacttgc tgctctggtt
      541 tgatattcca cgctttccac atagcggtaa tatcatcggc gctgcgcatg gtgccatccg
      601 ggttatgaaa atcttccata tgcgtcgagt cgctaccagc gtgtccccaa cgtgctccgg
      661 ctatttcacc ttttggttta atatagctgt aaccgctggt cgtaccgata aattctggcc
      721 acgaacgaat gctcaccagc gatgcatcct ggcgatgcag cagtccacgg gcctgttcca
      781 tatcgagcat caactgcggt tgtgccggga tcttcacgcc gaaatccggt tccgctttca
      841 ctttcggtgg cgttcctcgc tcaacaggca gtcccgcgtc ggaccaggtt tgccagccgc
      901 catccagcag acgcacatct ttcacgcctg cgtagagcat aatttgcgcc acacgcgctc
      961 cagcgtatac gtcacgcccg tacagaatga ccgtggtgtc atggcgaatg ccgtgttttg
     1021 ccagcatcgc ttttagttgt tcatcagaaa ctttgttcca cagcggttcg ctttccactt
     1081 cgttggtatc gatgtagtca gcaccgggaa tatggctgat aaggtaaagc ttaggagcgc
     1141 cccaggccgc ttcaatgact ttccagtcac cggcaggttt cgccgtaacc tctttacctt
     1201 gttgcaggtc gtgcagccat tgcggataaa ccagctgctc aaagtgcggc agtttttgta
     1261 gacgggaagg ttcgcttagc gcgtcactca gaatggagat atgcgtaaaa cctgcttttt
     1321 gcagtcgcgt tttgacggcg tcgacatctt tgtcattacc gtacagcgcc accggggcat
     1381 cggctttcag gttatgttgc ttgatccacg cgttgagctg ttcggtgctc attttgtcga
     1441 gccagctggc agagaggttt aaggcggcag gttcatgacc agaagggcca tttaaggttt
     1501 gtggccagcc gttataaaac gcgctggggc gagtatctat cgctttgcca ttttgttgtt
     1561 gaagctggtc aagtgtaaga ggcttcgcca gttcagcggc ccacgaagaa gcgcaagcca
     1621 gccctaaagc cattgccagc gcggtcattt gagaaacacg tttcatcgaa aatcccgaca
     1681 ttgtagtttt gagtgcgcca ttctgaactg acgcattgag aaaaactttg cgttatcgca
     1741 gtttgttgta attttctgac cactgcgcca tatccagaac agaactatca gcaggaacat
     1801 cctggagatc gtgcgttacc tgaacgacgg ggatcgccag ttcgcgaact tcgctgaaca
     1861 cccactggcg aaaattatcg cgcagagcca catcaagacg gctgaatggc tcatccagga
     1921 gtaacgcttt tggctgagcg agaagggcgc gcagcagggc cacccgcgct cgctgaccgc
     1981 ctgataatgt cgcagggtcc tggtgataag tttcagccag tcccgctcga tcaagcgcgt
     2041 ctttcacggc attgcgcctg gcagtcccct tgagcgtcga cagtagcgcc agcagtaaat
     2101 tttgcccgac gctgaactgg tcaaataaca gtgcatcctg gaaaagaatg ccaatctgac
     2161 gctgtgcggt gggtagcatg tcaatccgtt gctcattgag ccatagctca cctgtacaag
     2221 aaaactgtcc agccagtgca ccaatcatcc atgaaaacag agtggatttt ccacagccag
     2281 acggccccat taacgtgacg atgtcacctt tatcaaccgt aaagttaacg tttgtcagca
     2341 agcggctttc tggtaaacgt agcgaaacat ttttcacgca gagcattagc ggagtccttg
     2401 tctgacataa cctacccatt ttgcgactaa cgcggtcagg gcaaaaataa taagcggtaa
     2461 caacagttgc cataaagcct gggcggcgag aataccgttg ctgccgccgc tgcttaatgc
     2521 caccgcctca gtggtgagcg tcggaaaacg ccccgcgccc agccacagcg tcggcatata
     2581 ctgcgcaata ctgactgaaa atcccaccgc gaaggcaatc agcacagggc gcaacatgag
     2641 tgggcatttc acgtaaaaga agattttggc ccgcgaccag cccagtgttt gcgcaatcaa
     2701 aattaaccgt gaatcaatgc gctgccaggc gggttgcagg ataaacagca tccacggcat
     2761 tacccacagc agatgccccc agaccaccgc cgtccagctt ccatctaggt tcagccatag
     2821 cgccagcgtg tactggcccg ccaccagtgg cagatcgggc aatagaatgg gcaaccatag
     2881 ccacaactgg cgacgctgtg gcccccattc cagccacagc agtaacagga gcaaagcgat
     2941 gaatgttgct accagcccca ttgtcagact gttgatgagt gcttcactat tgatcgtcga
     3001 ctgatccgcg aggatcgcca gcagaaccac acacagcaca ccggttaagg gtaaaaaact
     3061 cgccagcgta gtgcctggta ataaaggtgt ggcaggcatt gcgaatgcca tctacgcggg
     3121 gaatagtgcg ccgccagctg cgccatagca gatagctcag caaaacgtag gcggcgagta
     3181 acagcatcag cagcagactg gcgagtgcgc ctttcgtttg ttgatccgcg tcgccctgag
     3241 ttaaccactg ccagctaatt actgccagcg tcggtggatt acctggccca agaataatcg
     3301 ccacatccac gaccgaaagc gaccaggcaa caatcgccag catcgccatt gccagcgaag
     3361 gcgcgacgga gggcaacagc agccagttca ggcattgcca gcggctgtag cccagcgaat
     3421 ccagcacaat gacctgctgc aacagccgtt tttcgctcaa tactgccgct aagatccaca
     3481 gcagaaatgc gctttctttc actgcaaggg tgaggcccag accgatgcca aatcggtcca
     3541 ttggcggagt gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca
     3601 gggcgctggt ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca
     3661 tacgctgcca tttcggccca ggccatagcg ccacaatcac cagcagggca atcaacaatg
     3721 ccccgaccgc cgcgatggtt gtcgatacca gcgttgccag taatgcatgc ggtaactgtg
     3781 gatcggcaaa taacgcctgc cagtgtgtca acgacaacgc aggcgatatc agcgtgagag
     3841 ctgccgggat cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg
     3901 cgtaccgtaa cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc
     3961 agccagtacc ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc
     4021 aggcagtttt tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg
     4081 cagttgcgca tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt
     4141 cgcgttggct ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa
     4201 actgtaactg cttgccggca aatcgccgct ggcgattttt tgctgcgcat gcgcggggtt
     4261 aaaggtcagc gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga
     4321 aggcgggaaa tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcca
     4381 caagggcgca gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg
     4441 atctggcgtc agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg
     4501 gcgcggataa gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt
     4561 ttgtggtggc tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca
     4621 gggcgattcc gcaccttgtg tcggcactga aaaatcttcc cgcaccggca gctgtgtgtc
     4681 gacatagcgc cagttgggca gagtctccgc ccagcccgtt tgtaataaat tggcctcttt
     4741 taaggtgcgg aagttttcgc cgttcaccca gagcagatcc accgagccgc ccgttttacg
     4801 accggcagca gcttcggtct gaatgcgctt caccgcgtct gcggcatccg cgagacgaac
     4861 aatcttcagg tttatagcgt aatgggtttt catctcgccg ctaacccagt cgagatagcg
     4921 gttaattgcg gtatcgccgc cccaggcgtt aaaccagacg gtttgccctt tggcctcatt
     4981 tttaatttcc tgccagtcac tggcatgtgt tgccaaagaa aacagcgata acaatcccag
     5041 caaccaccca caatggcgca tcttctctcc ttaagaacgt ttttccggaa tacggcacag
     5101 cccctgagcg ggaatgtcca tggcagaatt aaaacgagcc gacaggtttt gcaggccgat
     5161 caatgccgtc agttcggtga gcgcctgagc gtcaaaatgc gcagccagtc gggtacgcag
     5221 ggcatcatcg actgttggcg gtgtgacgct tgcggcttcg gcgtactcca gcgccagccg
     5281 ttcttcatcg ctaaagagcg ggctttgccg ccagtcagcc actgccagca gtttatcgct
     5341 gctgccggta cgctcggcga ctttcatact ggtgatatcc acacaaaact cacacaggca
     5401 catttgcgca atgcgcgcgc tgacaagcga tcgcactacc ggatcgagcg gtgagcgttt
     5461 gcgctccagc cacccaacaa acattgacac caaataaaag ataaacggga tccgccccca
     5521 ccagcgaatc gggcttagca cctcgccgta atggcgacga tgcagccacg cctgcggacg
     5581 taaaatgaaa ggaattttgc taagcggcgg taaacccatc agtcattcct cttctggcgc
     5641 tcaagccata ttttcttcgc cataaaaatc accacggtca gggcaaacag aataaacagt
     5701 ccggtgacaa accagaaagt accgcctgtt aacatgctgc ccgcccagga ataaacaata
     5761 gtcgtcggta attgaccaag cccggtggcg ataaaaaacg agcgaaaacg tattgaagtc
     5821 agatcggcag catagctgat tggatcgaaa gggacaaaag gcaataaccg acagaccagg
     5881 atggtgtgtt tgccgtagcg agtgaaaaag ccgtccatac tgtcaagcac ggttttgccg
     5941 gttaattttt ccaccacttc gcggcccatc actctggcga taaaaaagca cagcgccgcg
     6001 ccggccatcg aactggtcca cgacagcaag ccgccccaga acgcgccaaa cagcgacgca
     6061 ttggcaaagg tgatcaaaaa cgcaggtagc ggtgcagcaa tggcctgtaa aatcatcaac
     6121 aagaacgaga caactgccgc cagtgcgccg taagactgaa taaagcgttc tataccctgt
     6181 tggtccacgg cggcaaacgc cgcaacgctg cgattgataa actcatggac gccgggaagc
     6241 agcgcccatg cgagcagcat agcgaacaac aggatgatga gggttatacg gtagtaccag
     6301 attttacgcg attgcatctt catcatcctt tatcctggct ttattcggtg attttaaagg
     6361 taaccgttgc cagcgtgtta tccgccggga gaactgaggc attgcctttg aatttcactt
     6421 cgccgcgttt ttccaccgca ccataagtgt atgttgcatt gctgacgatg ccgaccgggc
     6481 agctatccag acacaccagg caacccgttt tcttctcttc tgctgccgtt aaattaccgc
     6541 caaagcgcat gtccagtttc ttgccattac tgtcaacaat cacttcatcg aaggaatacg
     6601 cttttgccgc cccttgccag ttgaccgaaa tatccagttt gctgcctgtg acatgagtcg
     6661 tttctttatt atccatcgtc atgttttcgc ccggcgtacc acctgcctct ttcagggctt
     6721 cataaaatgc tttcggggtc gcataagcca taaacagcga tttatgtccg ttggagccat
     6781 ctttaaacac aataccgtga cgagtgtcgt cggtgaggaa acgaccatta atttgcacca
     6841 acatggttac cgtatggtta ttggcatcga cgcgcagcgg ttggctgttc gaaagtccgt
     6901 cgtattccac ttttgccgcc gcattctctt tttgatcgca gccgctaagc ccggcaacag
     6961 ccagtaagca gagtgcagcc agtccttttt tccatgacac tgaataatgt tgcgacatcg
     7021 ttctatcctt cattttttgg gtgagtaagc gggctgcggc gcgaagcaga cagatccaca
     7081 tgtttgtgtc gggcgtagag ttttgcgagc tggacgagaa taaacagcgc caggcccgcc
     7141 agacagagtt gtaaaataaa gcgcagcgta atgccttcat tggcgagatc gcttgccatc
     7201 acggtataaa taacaatacc gggcagggtc gtgagtgccg aaataagggt ataaggccag
     7261 aaggcgatgg tggttaatcc gtaagcgtaa ttttgaatat tgtaaggaaa caacgggatt
     7321 aagcgggtca gaataagaaa atcaataccg ttacgcgcaa tgcctttttc aatggcctgg
     7381 aaggtatggc tatggcccac gtatttcagc agtaaatcac gtcccagcca ccgcgccagc
     7441 aggaatgagc acgaggaggc cagcgtggcg gcaattaatg agagtagtgt ccctaagagc
     7501 gggccaaaaa ctattcctcc agcgatcacc agtatgcttc ctggtaacac aaagagggtg
     7561 gcaatgatga ataacagaat atagaggcta taaccgaaca atccgctctg gcgaatgagt
     7621 gtctgtaagt ggggtaaatc ggtgagcaga tcgaataaac cgaaagcgtg gatagcgtaa
     7681 atgaccagcg caaaaataag acaggcgaaa agaaatttac gctcagcatt catcgtggag
     7741 tttcctatat caaacttgat aatagagaag tggtcagaca actaaaaacg cagtgacggt
     7801 ataacaaagg acggcaggca acaaatcatt actgacagtt tgttgatttt tctcaaggtt
     7861 aattctcctg acccagtttg agccaggaga gctgctaaat tagcggcgga aggtcgccca
     7921 gacgggggcg tgatcggacg gtttttccat gctgcgaatt tcatagtcga tgccggtttc
     7981 tacgcaacat tctgccagcg gttggctggc gagcagcagg tcgatgcgca gaccacggtt
     8041 atcgtcaaaa ccttttgagc ggtaatcaaa ccatgagaaa cgatctgctg tttgcggatt
     8101 agcatggcgg aaggtatcga ccaaccccca gctcatcagc ctgtccatcc attcgcgctc
     8161 ttccggcagg aaagagcatt taccggtacg cagccagcgc ttacggtttt cttcgccaat
     8221 gccgatatcc agatctgtag ggctgatatt catatcgccc ataatcagta ccggattatc
     8281 acgtttgagt tcggtttcca ggtagttttg cagattctga taaaactgcg cttttgccgg
     8341 gaattttatc ggatggtcgc ggctttcacc ctgcgggaag taaccgttga tcacggtgac
     8401 attacccagc agtgagggga tttccgccat aataatccgc cgctgcgcct cttcgtcgtc
     8461 accgggaaag ccgcgacgca cggcaatcgg cgtctctttg gtcagcagcg ccacgccata
     8521 atggcctttc tgcccgtgat aaaacacgtt gtagccgagc ttcgccacct cttcgagcgg
     8581 aaacatatcg tcatgaactt ttgtctcctg caggccaatc acatccggtt ggtgcttttc
     8641 gacgatggct tcaagctggt gaggtctggc gcgcaggccg ttgatattaa aagagacaaa
     8701 tttcatagtc gctgccattt attcagataa agagtgcgtg gatggtagca gaatttcgcg
     8761 ttgcttaccg cctgttgtta agtgatttga tcgatggttg ttaatgatgt caacgatggc
     8821 gcaaaaaatg cccgcttttg gtgcgcgctg cgtcagaatg gcgcagtaat ttccagtaaa
     8881 tttcgtcaat gatcacaatt ccagaattat attttacttc tgcataacat tgcgtttttt
     8941 attcttttta ttcacccaca tcgcaaacgt attcacttta tatgcacttt aaatgcatat
     9001 ggtttggtta taactccttg atttccagtt agcctccgcc gtttatgcac ttttatcact
     9061 ggctggcacg aaccctgcaa tctacattta cagcgcaaac attacttatt gttaacatat
     9121 agatagcgaa ttatttactg tagaggtcgc tatgtctcag ccaattacgc gtgaaaactt
     9181 tgatgaatgg atgatacctg tttacgctcc ggcacccttt ataccggtac gtggcgaagg
     9241 ttcgcgcttg tgggatcagc aggggaaaga gtatatcgac ttcgcgggtg gcattgcggt
     9301 gaacgcgctg ggccatgcgc atccggaact gcgtgaagcg ctgaacgaac aggcgagtaa
     9361 gttctggcat accggcaacg gttacaccaa cgagccggta ctgcgactgg cgaaaaaatt
     9421 gatcgacgcc acgtttgccg atcgcgtctt cttttgtaac tccggtgcgg aagccaacga
     9481 agcggcgcta aaactggcgc gtaaattcgc tcacgaccgc tacggcagcc ataagagcgg
     9541 tatcgtggcg tttaaaaatg cgtttcatgg tcgcacgctg tttactgtca gtgcgggtgg
     9601 gcagccagcc tattcacagg attttgcgcc actgccacca gatatccgtc atgctgcata
     9661 taacgatatt aactctgcca gcgcgctgat tgacgacgcc acctgtgcgg tgattgtcga
     9721 acccatccag ggggaaggcg gtgtggtgcc agccagcaac gcgtttttac aaggtctgcg
     9781 tgaattgtgt gaccgccaca atgcgctgtt gatttttgat gaagtacaaa ccggcgtcgg
     9841 gcgcaccggg gaactgtatg cctatatgca ctacggcgtg acacctgatc tgttaactac
     9901 cgccaaagcg ctgggcggcg gtttcccggt cggtgcgttg ttaacaaccg aagagtgcgc
     9961 cagcgtgatg accgttggca ctcatggcac cacctatggc ggcaatccgc tggcctcggc
    10021 ggtggcgggc aaagtgctgg agctcatcaa cacaccagag atgcttaatg gcgttaaaca
    10081 gcgtcacgac tggtttgttg agcgtcttaa taccattaat caccgctacg gtttgttcag
    10141 tgaagttcgc ggcttaggtt tgttgattgg ctgtgtactg aatgccgatt acgccggaca
    10201 agcgaaacag atctctcagg aggcggcgaa agcaggcgtt atggtactga ttgcgggtgg
    10261 caacgtggtg cgttttgcgc ctgcgctcaa tgtcagcgaa gaagaggtga cgaccggact
    10321 ggatcgcttt gcagctgctt gcgaacactt tgttagccga ggttcatcat gatggtcatc
    10381 cgtcccgttg agcgctcaga tgtctcggcg ctgatgcagc ttgccagcaa aacgggcggc
    10441 ggcctgacgt cgcttcccgc caatgaagcc acgctttccg cgcgtatcga aagggcaatc
    10501 aaaacctggc aaggcgaact gcccaaaagc gagcagggct atgtgttcgt gctggaagat
    10561 agcgagacag gcaccgtggc ggggatttgc gccattgagg tggcggttgg gctgaacgat
    10621 ccctggtaca actatcgggt cggcacgttg gttcacgcct caaaagagct gaatgtctat
    10681 aacgcattgc cgacgctgtt tcttagtaac gatcacaccg gcagcagcga gctgtgcacg
    10741 ctgtttctcg acccggactg gcgcaaagag ggcaacggct atttgctgtc gaaatcgcgc
    10801 tttatgttta tggcggcttt tcgcgacaag tttaatgaca aagtggttgc tgaaatgcgc
    10861 ggggtgattg acgaacacgg ctattcaccg ttctggcaaa gcctcggtaa acgcttcttt
    10921 tcgatggatt ttagccgcgt cgattttctc tgcggcaccg ggcaaaaggc atttattgca
    10981 gaactgatgc cgaaacatcc gatctatacc cactttttat cccaggaagc ccaggacgtc
    11041 atcggtcagg tacatccgca aaccgcgcct gcccgcgcgg tgctggagaa agaaggtttt
    11101 cgttaccgca actatatcga catcttcgac ggcgggccaa cgcttgagtg tgacatcgac
    11161 cgcgtgcgcg ccatccgtaa aagtcggctg gtggaagtgg cagaagggca gcctgcgcag
    11221 ggcgattttc cggcctgcct ggtcgccaat gaaaattatc accatttccg cgtggtgctg
    11281 gcgcgtaccg atccggcaac cgagcgtttg attttaaccg ccgcacaact ggatgccctc
    11341 aaatgccacg caggggatcg cgttcgtctg gtgcgcctgt gcgcagagga gaaaacagca
    11401 tgactttatg gattaacggt gactggataa cgggccaggg cgcatcgcgt gtgaagcgta
    11461 atccggtatc gggcgaggtg ttatggcaag gcaatgatgc cgatgccgct caggtcgaac
    11521 aggcttgtcg ggcagcccgt gcggcgtttc cgcgctgggc gcggctctca ttggctgaac
    11581 gtcaggtcgt tgtcgaacgc tttgccggat tgctggaaag caataaagcc gaattaaccg
    11641 cgattattgc cagagaaacg ggtaagccgc gctgggaagc ggcaaccgaa gtgacggcga
    11701 tgatcaataa aatcgcgata tcaattaagg cgtatcacgt tcgtaccggc gagcagcgta
    11761 gtgaaatgcc ggacggtgcg gcgagcctgc gacatcgccc gcacggcgtg ctggcggtgt
    11821 ttgggccgta taatttccct ggtcatttgc cgaacggaca tatcgttccg gcattgctgg
    11881 caggtaacac cattatcttt aaacccagcg aactgacacc gtggagtggc gaagcggtaa
    11941 tgcgtttatg gcagcaggct ggcttgccgc cgggcgtgct gaacctggtg cagggcgggc
    12001 gtgaaacggg tcaggcgctg agtgcgctgg aggatctcga cggtttgctg tttaccggta
    12061 gcgccaatac cggctaccag ctgcatcgcc agctctccgg tcagccggag aaaattctcg
    12121 cccttgagat gggcggtaat aatccgctaa ttatcgatga ggtggcggat atcgactcgg
    12181 ctgtccatct gaccattcag tcggcgtttg tcacagccgg gcaacgctgc acctgcgccc
    12241 gccgtttatt gctcaaaagc ggagcacagg gcgatgcgtt tcttgctcgt ctggttgccg
    12301 tcagccagcg attaacgccg ggcaactggg atgacgaacc gcagccgttt attggcgggc
    12361 tgatttctga acaggccgca cagcaggtgg ttactgccta gcagcaactg gaagcgatgg
    12421 gcggacgaac cctgcttgcg ccgcgcttat tacaatcaga gacatcgttg ctgacgccgg
    12481 ggatcattga aatgacaggc gttgctggcg taccagatga agaggtgttc ggaccgttat
    12541 tgcgcgtctg gcgttatgat tctttcgagg aagcgattct aatggcgaat aacactcgct
    12601 tcggactctc ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa ctgttgctgg
    12661 aggcgcgggt ggggattgtt aactggaaca aaccgcttac tggtgctgcc agtaccgcgc
    12721 cattcggcgg cattggtgca tccggtaacc atcgccccag cgcctggtat gccgcagatt
    12781 actgcgcatg gcctatggcg agcctggagt cggactcgtt aactttgccc gcaacgctta
    12841 accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag ttaatttcga
    12901 cgggctggta gggctgacgc atcattacgc tggcctgtcg tttggtaatg aagcctctac
    12961 ccgtcaccgt tttcaggtgt ctaacccgcg actggcggcg aaacagggct tactgaaaat
    13021 gaaaaccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg agcgtccgtt
    13081 tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac tggaaaaagt
    13141 tgcacgccag gcaccgcact ggctttccag cgtcagctcc gcttcgccaa tgtgggtagc
    13201 caatgcggca acgatcgcgc catctgccga tacgctggat ggcaaagtgc atctcaccgt
    13261 tgccaacctg aacaataaat ttcaccgttc gctggaagcg cccgtcactg aatcgctgtt
    13321 aaaagctatt tttaacgacg aagagaaatt tagcgtccat tcggcgttgc cgcaggtagc
    13381 gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt acggtgagcc
    13441 gggtatgcaa ctttttgtct acgggcgaga agaaggcaat gatacccggc cttcccgtta
    13501 tccggcgcga cagactcgcg aagccagcga ggcggtggca aggcttaatc aggtgaatcc
    13561 ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagggtg tttttcataa
    13621 tgacgtgatt gccgtaagta accgccaggt gctgttttgc caccaacagg cgttcgctcg
    13681 ccagtcacag ttactggcaa acctgcgggc gcgggtgaac ggttttatgg cgatagaagt
    13741 tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta acagccaact
    13801 gttgagccgc gatgatggtt ccatgatgtt ggtgctgcct caggagtgtc gggaacacgc
    13861 cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta gcgaactaaa
    13921 agtctttgat ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc tgcggttgcg
    13981 ggtagtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga tgaacgatac
    14041 gctgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgcc ttactgctgc
    14101 cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg tattgagcca
    14161 attactgaat ctcggttcgg tttatccgtt ccagcgcgag ggagggggca atggataatt
    14221 ttcttgctct gaccttaacg ggtaaaaaac cggttatcac cgagcgagaa atcaacggcg
    14281 ttcgctggcg ctggctgggc gatggtgtgc tggaactgac gccattaacg ccaccgcaag
    14341 gcgcgctggt gatttctgcg gggatccacg gtaatgagac ggcacctgtg gaaatgctgg
    14401 acgcgttgct tggcgcgata tctcacggcg agatcccgtt acgttggcgg ttgctggtga
    14461 tcctcgggga tcctcctgcg ctgaagcaag ggaaacgtta ttgccatagc gatatgaacc
    14521 gaatgtttgg cggtcgttgg cagctatttg ctgaaagcgg agaaacctgt cgggcgcgcg
    14581 aactggaaca gtgcctggaa gatttttatg accagggcaa agaatctgtg cgttggcacc
    14641 ttgatctaca taccgcaatt cgtggctcct tgcatcctca gttcggtgta ttaccgcaac
    14701 gcgacattcc ctgggacgag aaatttctga cgtggctggg tgcggcgggg ctggaggcgc
    14761 tggtgttcca tcaggagcct ggtggtacgt ttacccattt cagcgccaga cattttggcg
    14821 cgctggcctg tacgctggaa cttggcaaag cgttgccctt tgggcaaaac gatcttcgcc
    14881 agtttgcagt aactgccagc gcaattgctg cgctgctatc tggtgagagt gtcggtatcg
    14941 tgagaacacc gccgctccgt tatcgggtgg tttcgcaaat tactcgccac tcgccgtcct
    15001 tcgaaatgca tatggcaagt gacacgctga attttatgcc gtttgagaaa ggaacattgc
    15061 tggcgcagga cggagaggaa cgttttaccg taacccatga tgtagagtat gtgttattcc
    15121 ctaatccgtt ggtagcgttg ggattacgcg cgggattaat gctcgaaaaa ataagctaat
    15181 acttacccgc agaaatcatt ctgcgggtat taagttgctt tcttataaat t
//
LOCUS       DAZOZY010000093        15177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZOZY010000093 DAZOZY010000000
VERSION     DAZOZY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.093"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(8..1993)
                     /gene="yjcS"
                     /locus_tag="ACHURQ_002463"
     CDS             complement(8..1993)
                     /gene="yjcS"
                     /locus_tag="ACHURQ_002463"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR0824210.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            2246..2563
                     /locus_tag="ACHURQ_002464"
     CDS             2246..2563
                     /locus_tag="ACHURQ_002464"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR0824211.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            2632..2961
                     /gene="yjdP"
                     /locus_tag="ACHURQ_002465"
     CDS             2632..2961
                     /gene="yjdP"
                     /locus_tag="ACHURQ_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR0824212.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(3108..3866)
                     /gene="phnP"
                     /locus_tag="ACHURQ_002466"
     CDS             complement(3108..3866)
                     /gene="phnP"
                     /locus_tag="ACHURQ_002466"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR0824213.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(3868..4302)
                     /gene="phnO"
                     /locus_tag="ACHURQ_002467"
     CDS             complement(3868..4302)
                     /gene="phnO"
                     /locus_tag="ACHURQ_002467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR0824214.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            complement(4289..4846)
                     /gene="phnN"
                     /locus_tag="ACHURQ_002468"
     CDS             complement(4289..4846)
                     /gene="phnN"
                     /locus_tag="ACHURQ_002468"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR0824215.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(4846..5982)
                     /gene="phnM"
                     /locus_tag="ACHURQ_002469"
     CDS             complement(4846..5982)
                     /gene="phnM"
                     /locus_tag="ACHURQ_002469"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR0824216.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            complement(5979..6659)
                     /gene="phnL"
                     /locus_tag="ACHURQ_002470"
     CDS             complement(5979..6659)
                     /gene="phnL"
                     /locus_tag="ACHURQ_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR0824217.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            complement(6770..7528)
                     /gene="phnK"
                     /locus_tag="ACHURQ_002471"
     CDS             complement(6770..7528)
                     /gene="phnK"
                     /locus_tag="ACHURQ_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR0824218.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(7525..8370)
                     /gene="phnJ"
                     /locus_tag="ACHURQ_002472"
     CDS             complement(7525..8370)
                     /gene="phnJ"
                     /locus_tag="ACHURQ_002472"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR0824219.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(8363..9427)
                     /gene="phnI"
                     /locus_tag="ACHURQ_002473"
     CDS             complement(8363..9427)
                     /gene="phnI"
                     /locus_tag="ACHURQ_002473"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR0824220.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(9427..10011)
                     /gene="phnH"
                     /locus_tag="ACHURQ_002474"
     CDS             complement(9427..10011)
                     /gene="phnH"
                     /locus_tag="ACHURQ_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR0824221.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(10008..10460)
                     /gene="phnG"
                     /locus_tag="ACHURQ_002475"
     CDS             complement(10008..10460)
                     /gene="phnG"
                     /locus_tag="ACHURQ_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR0824222.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(10461..11186)
                     /gene="phnF"
                     /locus_tag="ACHURQ_002476"
     CDS             complement(10461..11186)
                     /gene="phnF"
                     /locus_tag="ACHURQ_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR0824223.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(11207..11986)
                     /gene="phnE"
                     /locus_tag="ACHURQ_002477"
     CDS             complement(11207..11986)
                     /gene="phnE"
                     /locus_tag="ACHURQ_002477"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR0824224.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(12112..13128)
                     /gene="phnD"
                     /locus_tag="ACHURQ_002478"
     CDS             complement(12112..13128)
                     /gene="phnD"
                     /locus_tag="ACHURQ_002478"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0824225.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(13153..13941)
                     /gene="phnC"
                     /locus_tag="ACHURQ_002479"
     CDS             complement(13153..13941)
                     /gene="phnC"
                     /locus_tag="ACHURQ_002479"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR0824226.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(14074..14517)
                     /gene="yjdN"
                     /locus_tag="ACHURQ_002480"
                     /pseudo
     CDS             complement(14074..14517)
                     /gene="yjdN"
                     /locus_tag="ACHURQ_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            complement(14675..15010)
                     /locus_tag="ACHURQ_002481"
     CDS             complement(14675..15010)
                     /locus_tag="ACHURQ_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR0824227.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         3083 a         4187 c         4480 g         3427 t
ORIGIN      
        1 agtgacatta atttggggtt acgatattca cccacaaatc gaaattatcc agacaggcga
       61 taatttcttc cagtttcgcg ccattgccaa taattttggc tttcttcgct tttaccagat
      121 ccgccatttt ggcttgcccg gtcagcacgg cgtgcagatc ttcacggctg atgtagaaag
      181 aggcgtcagc ttgggattgc agtgttttac ggtagttaag cacgctatcg ttcagcgtca
      241 ggttgaggtt atcgccattg ctcatattga agttcaggct gatattttta cccgcggctt
      301 tcgcgctatc gaggcgaacg gacataaagt cgaacagcat ttcgaccgac atcccgcgaa
      361 tggtgtccgg ggaaccgatg gtgccgtggc tgaacttatg caccccttcg cgcagctctt
      421 tcgcgccggt caggtagaaa ccgcgccagg tggcggactc ggcctgatag cccagctgtt
      481 caaagttatt cgcttgcaga ttcttcgcga cctggtcacc cgggttggcg gcaatcacct
      541 gtttcagcag ttctgccgac cagcggtaat caccttgctt gttcgcttct tgcgccaggt
      601 tgatgacacg ggcagaaccg cccagcgcct gcacgtaacg tttacccatc tccacctgac
      661 catacggatg caggttagcc gggttaccgt cgtaatagcc aagatagaag ttatacaccg
      721 cgcgggcgtt gtggctgaca gaaccgtaat agccacggct ggcccagtta ttggcaagtg
      781 caggcggcag tttaatcatg tcgccgattt cattcatggt gtagccctgg ttcgccaggt
      841 gcagggtctg gtcgtgaatg tacttgatag tatcgcgata tttaccaata taatcattga
      901 tatgcttatt gccccagacc ggccaggtgt gcggcataaa caggacttcc gcgtcgttac
      961 cccacatatc cagcgtttcg ttcagatact cggtccactt gctggtgtcg cgggttttcg
     1021 cgccgcgcag agtgtagaag ttgtgcaggg tatgcgtggc gttctcggcg gtacacagcg
     1081 ctttcagggc cggaatatag aagtgcattt cggctggtgc ttcgctacct ggggtcatca
     1141 ggaagtcaaa ctccaggccg tcgataatca tcttctcacc ggttttggtg atggttttgt
     1201 tcggtgcgat gatgctcggg tagcccgatg ccagcgttac gccaagacca ttgccgatgt
     1261 taccctgagg gttatgcgcc agcagcaggc cgtaggagta aagtgcacgg cggctcataa
     1321 tattacccgc cagtacgttt tcgctgatgg cttcgtccat aaagccagca ggggcgataa
     1381 cctgcacttt accggatttc acatcagctt cagagataat gcctttcacg ccgccatagt
     1441 ggtcggtatg gctgtgggta taaataaccg caacaatcgg tttttgcggg cgattttgga
     1501 agtaaaggtc aagggcggct tttgcgctcg gtggcgtcac cagcgggtcg atgacaataa
     1561 tcccggtatc accttcgata aaggtgatgt tggagatatc ctgaccgcga acctggtaca
     1621 ttctgtcggt gactttaaac aggccggaaa taccgtttaa ctgcgactga cgccacaggc
     1681 tagggttaac ggtttcgggc gcgggcgcat tgatatcaaa tttataatct tccccgcgat
     1741 aatatggttt gccattagca tcgcgcagaa taccttcatc cagcagcgga gcgataaatc
     1801 cacgctgagc gtcgtcaaaa tcctgacgat cgctaaacgg taatgatttg gcgtaattct
     1861 gattaacctg ttgggtatat tgagtggcgg ctttcgcttc ctctttcgcg ttagcggtat
     1921 ttaccatcat gccgctggcg gcagttaacg caataacaat tctgctcaaa cggaataacc
     1981 gagagttatt catgtaaatc tccatttatc atttatgtcg taaatatgta atccatgcaa
     2041 aatacgtcat ttaaagctgg ggttatcata attgggaatt tgcgacttta ataagtggaa
     2101 gtgtgagctg aacgatccat tttattaggc atttgtgctg aagagggaaa attacgcgaa
     2161 tttattatct ctgtaaataa tatgaggtgt tttatatatt tattatatat attgtacttc
     2221 ggcaaatact gagcacaatt tatcaatgca accggtgttt ctttttaaaa aacgtaccgg
     2281 agcaatgggc ggtcatgtcg ctggcaccgc aggattctgc gttgctgtgc cagcctttta
     2341 ttcatggtca atgagtgagg tgattgataa agacaccagg tcgtcagagc gcactgatta
     2401 tccgcattat gccagtgaag ttgcactggc ttttggttca cgagtggttg gcctcgaact
     2461 ggcaaaaatg attgtggatg cgtggctggg cgcacagtac gaaggcggtc gtcatcaaca
     2521 acgcgtggag gcgattacgg caatagagca gcggagaaat tgagattcat ccactacttg
     2581 catggatgag taatgactaa tgtggataga gtttcttttt gaggttggct aatgaaacgc
     2641 tttccgctat ttcttttatt caccctgctc acgctgtcca ccgttccggc ccaggctgat
     2701 attatcgacg atactattgg taatattcag caggcgatta atgacgctta taaccctgac
     2761 cgtgggcgag actatgaaga ttcgcgcgac gacggctggc agcgtgaagt gagcgacgat
     2821 cggcgcagac aatatgacga ccgccgccgc cagtttgaag atcgccgcag gcagctggac
     2881 gatcgccagc gccagcttga tcaggagcgt cggcaactgg aggatgaaga gcggagaatg
     2941 gaagatgagt atgggcgatg aggttgggta tggagcaggc atcgcccctc accccaatgg
     3001 ggagagggag aaaacgagcg caatattcaa tatcgagcac aatcggtccc ctcgcccctc
     3061 tggggagagg gttagggtga gggggccagc gcgcacattc cctctcatca cgccacccca
     3121 atctccatcc catcaaaccc cacctcaaac cctgacggta gtgcgttttc catcagccac
     3181 gcatcaaact ggtggctgat atgggtcaga atcacccgtg gcgagcggat aacctgattc
     3241 agcgcaagca cggtatttaa atcacagtga ttacgcggtg catccgcgcg cggcgggtga
     3301 ctgcaatcca tcaccattac ctgcggctga ttattgcgta aaaatttcag cgttttttcc
     3361 ggcaagccag cggtgtcaga cagccacgcc acccggctgt gcgccgtttc cagtaaatag
     3421 ccgaaagtga gttttgagtg gttaagcggc agcggcgtga cctgcaaccc ctgcaaatca
     3481 aacaccacaa acggttccac cgtgtggctg aaatcaagca gcccgggatg tttaaacaga
     3541 tcgtcgcagc cctgttcatc cggcgggccg tagatcggga tcggatcgcc cacgccccag
     3601 cgcagcggaa acagcccctg gacgtgatcc atatgataat gcgtcagcaa aaactgctgg
     3661 aacgatccgg gagaccagcg atcggtgaga tcgtgcagcc cggcgtcgat cagggtgatc
     3721 gcctcgttaa acttcactac gccgctgcac ggttggcggc gatactgcgg cgagcgccgc
     3781 gctcggtcgc aggccgcgca ctcgcagccc catgccggaa cgccctgtgc gccgccggtg
     3841 ccggtgagcg tgagggtcag actcatgtta cagcgccttg gtgaagcgga agtgactctg
     3901 ctcgtagcct tctcgcagat agaaacggtg cgcgtcgtgg cgcttcacgt tggtcgaaag
     3961 ctcggtcatt tcggccccgg cctggcgggc ttcttcttct gcccacgcca gcaacttgct
     4021 accgacgttc aggccgcgcg cctgcggcat cactaccagt tcctgaattt cgccgatcca
     4081 gttgacatga tgcagatgaa actgcaaatg caggccgatc atgccgacaa cctcgccatc
     4141 cagcagcgcc agatgatagc gcatgttcgg gtcgcgcaga ttggcgctaa agcccacgcg
     4201 aaatgcgtgg tggtcaaact ccgcctgttt tagctcacaa atcagcgcgt aaaccgcgtc
     4261 ggtatcatac tgcgtggccg ggcgaagctc acaagcaggc atggtgtttc tccttctgat
     4321 gaatcagcgt cagcagcgtg tcgaccgact ggcgcaggct gccgtcattg ttgagcgtat
     4381 ggcaatcctg tggagtatag cgggcggcgc gcgccaggcg ggcgttaatt tcactggcgt
     4441 tttcacggcc acggttttcc aggcgctggc ggaggatctc cggcgaaacc tgtaaacaga
     4501 cgggcagcag cgccgattga tagcgcgccc gcgcctgcgg cagatgggcg cgtgagccgt
     4561 tgaccagcac gtcgaatccg gcgtgcagcc agaggtcgat ctcgacgccg acgccataat
     4621 acagaccgtt agcgtgccag cttaaggcca gtaggttctg ccccgcgcgg gtaaaaaact
     4681 cctgctcgct cagagcgata tggttttcgc ttccggcgct ggcgtcgcgc gtgatgtagc
     4741 gatgcgccac cagtagctga gtttgttccc gcaggcggag ttccgccagc aggctatctt
     4801 tcccggagcc ggacggcccc attaaccaaa tcagttttcc catcatcaga acaccctttt
     4861 accctgacgc cagacgtggt cgatatgaat gtgattgccc ttgcgatgcg ccagcaccaa
     4921 gtccgcgcgt ttgccctcgc caatcacccc gcgatcctgg agattaagcg cctgcgctgg
     4981 atttttagtc accagcttca ccgcctgcgg cagcgtaaag cggttgctct cgtcatcggc
     5041 gacgcgaaat gccgcatcga gcaggctggc ggggtagtag tcggaagaga ggatatccag
     5101 caggccaagc tgcgccagtt cactggctgc cacgttgccg gagtgcgagc cgccgcgcac
     5161 aatattcggc gcgcccatca gcacgttcat gccatgcttg cgcgaggctt ccgccgcttc
     5221 gaacgtggtg ggaaattcgg cgatcacgct gccaagctgg tgagattcag caacgtgggc
     5281 gtgggtggcg tcatcgtggc tggcaagcgc aatttttcgc gcgcggcaca gggcggcgat
     5341 ggattcgcga ttcggctgcg accagcgtgc ggcgagcgcc agttgctctt cttcgtactg
     5401 ctgcatctgc gcatcagtga gggagtattt gccctgataa tattcgcgat acttctcgcg
     5461 attggcgaac tggcgctggc ccggcgagtg gtccatcagc gacaccagcg tcaccggctc
     5521 gcgctgcacc agtttttcaa acagcggcag cgtggtgtga tgcggcagtt cgcagcgcag
     5581 atgcagacgg tgctcggcgc ggttgacgcc gcgtttctgc gtctcttcga tggcgttgat
     5641 catcttctcc agattcacca gccgatcgcc gccgtcgcgc acgtcgccaa ttgccacggc
     5701 atccagtacg gtggtgatgc cgctcgccac catcagcgcg tcgtggctgc tcatcgccga
     5761 gtgggcaggc cagtcaactt tcgggcgcgg ggtgaagaat ttatccagat tatcggtatg
     5821 cagctcaatc agccccggca gcagccagcc gccttcgccg tccatcgcct ccggcaggcg
     5881 gctctggctt tcggcaaagg cgcggatttc gccgtcctgc acctccagtg aaccgtgtac
     5941 cacttcgttt tccagcacca atttgacgtt attgataatc atgaagaggc ttccattggg
     6001 tgcaggcggt cggcaacgtc attacgtacg gcttcgtcgt ggaagatgcc gacgatggcc
     6061 gcgccacggg ctttggcttc gtgaatcagc tcgactacgg cggcgctgtt tttggcgtcc
     6121 agcgaggcgg taggttcgtc gagcagcaga atggggtagt cgacgataaa gccgcgggcg
     6181 atgttgacgc gctgctgttc gccgccggag aaggtcgatg gcgccagatg ccacaggcgt
     6241 tccggcacgt tcaggcgggt gagcagacgc gcggctttag cggcgcaggc ttcacgcggt
     6301 acgccggtat cgagtagcgg ctgcatcacc acttccagcg ccgaaatacg cgggatgacg
     6361 cgcagaaact ggctcaccca gccaacggtg gttttgcgga tctccaccac tttgcgcgct
     6421 ggcgcggtga ccagatcgac ccattcgtca ccgtgtttga tctggatttg cccttcgtcg
     6481 ggcagatagt tggcgtacag cgagcgtagc agggttgatt ttccgctgcc ggaatggccg
     6541 tggagcacca cgcattcgcc cgcgttgacg atgagcgagg cgcggttgag gacgggcagg
     6601 cgcacgccgt tttgctggtg caggatgaag gttttactga cgttttgtac gttaatcatt
     6661 tttcgcctcg tggttcgggt gtcggatgcg acgctgacgc gtcttatccg acctacgggg
     6721 agcgcatttg taggccggat aaggcgttta cgccgcatcc ggcaccggct caattctgca
     6781 aaaccgatga caccagcagc tgtgtatacg gatgatgcgg gtcgtcgagc acgcggtcgg
     6841 ttaacccact ctccaccact tgcccctgct tcatcaccag caaacggtcc gccagcaggc
     6901 gggcgacgcc taaatcgtgg gtgacaatca ctaccgcgag gttcagctcc accaccagtc
     6961 cgcgcagcag gtcgagcagg cgggcctgca ccgacacatc cagcccgccg gtcggttcat
     7021 ccataaacac cagcttcgga tgcgtcacca ggttgcgggc aatctgcaaa cgctgctgca
     7081 taccgccgga aaaggtggtc ggcaggtcgt cgatacggtt ggcgggaatc tccacctctt
     7141 ccagccactt ctgcgcggta gcgcgaatat cgccgtaatg tcgcgccccg gtcgccatca
     7201 gccgctcgcc gatattgccg cctgccgaca cctggcggcg caggccgtcg agcggatgct
     7261 gatgcaccac gccccattcg gtgcgcagca ggcgacggcg gtcggcctcg ctcattgcat
     7321 agagggaacg gttttcatag tgaatctccc cctgctgcgg cgtcagacgc gcggagatcg
     7381 acttcagcag cgtggtcttc ccggagccgg attccccgac aatgcccagc acttcccccg
     7441 gccataaatc aaaagagaca tcgctaaagc ctttgcccgg cgcgtaaagg tgggtcaggt
     7501 tattgaccga aagtaacggt tgattcattg gcttttcgcc tcgctctgtt ggcggcaata
     7561 atcggtatcg gagcagacaa acatgcggtt tccggcgtca tccagcacca cttcatcaag
     7621 atagctgtgg gtcgatccgc agatggcgca tggctcatcc cactgctgaa cggtgaacgg
     7681 gtgatcgtcg aaatcgagac tttccacgcg ggtaaacggc ggcaccgcat agatgcgctt
     7741 ctcgcgccct gcgccgaaca gctgcagggc gggcatcatg tccattttcg ggttatcgaa
     7801 tttcgggatc ggcgacgggt ccatcacgta gcgcccgttc accttcaccg gataagcata
     7861 ggtggtggcg atatgaccga agcgggcgat atcttcatac agtttcacct gcatcacgcc
     7921 gtactcctcc agcgcgtgca tagtgcgggt ctccgtttcg cgcggctcga taaagcgcag
     7981 cggttccggg attggcacct ggaagataat gatctgatct tcggtcagcg gcgtttcggg
     8041 gatgcggtga cgcgtctgga taagcgtcgc atcgtccgta cattcagtgg tgtttacccc
     8101 ggtcacgcgc ttaaagaagt tgcgaatcga cacggcgttg gtggtgtcat ccgcaccctg
     8161 gtcaatcacc ttcaacacgt cgctttcgcc aatcacgctg gcggtgagct ggataccacc
     8221 agttccccag ccgtacggca tcggcatctc gcgcccgcca aacggtacct gataaccggg
     8281 gatcgccacc gcttttaaga tggcgcggcg gatcatgcgt ttggtctgct cgtcgaggta
     8341 ggcaaagttg tagccgctca gattagccat ggttctgctc ctgttgcaga cgtttgagta
     8401 gctccagttc ggcctggaaa tcgacgtagt gcgggagttt gaggtgcgag acaaagcctg
     8461 cggcttcgac gttgtcggca tgcgccagca cgaactcttc atcctgcgcc gggcctgtcg
     8521 cgtgctcgcc gtattccggg gcttgcaacg cacggtcaac cagcgccatt gccatcgctt
     8581 tgcgctcgct catgccgaat accagcccgt agccgcgcgt gaagtgcggc ggctcatccg
     8641 gcgggtcgat aaaaccgttg accatttcac actcggtcat cagcagttcg ccgacgttta
     8701 ccgcaaatcc cagctcttcc ggcacaatcg acacgtcgat gtaaccactg cggatctcgc
     8761 ccgcgaacgg gtggttgcgc ccgtaaccgc gctgggtgga gtaagccagc gccagcaaat
     8821 agccttcgtc gccgcgcatt aactgctgca aacgggaaga acgtgaacag gggtaaaccg
     8881 gcggcgtgcg ggtgatgtca tccggctgtg ctccgctatc ctcttcaaac ttcgccagcc
     8941 cctgacgcgc cagcaggctg aaaacgtgcg gcgacggctg ttgttcgctg tcggcggtgg
     9001 tcagcgtcgg cgcttcgccg tttgccagca gagtgaaatc gagcaggcga tgggtgtagt
     9061 cgtaggttgg gccaagcagc tggccgccgg gaatatcttt ataaaccgct gaaatacgcc
     9121 gttcgagacg catctccgtg gtgtcgagcg gctcgcttac cgccagtttc gtcagcgtgg
     9181 tgcggtaggc gcgtagcagg aaaatcgctt cgacgttatc gccgctggcc tgtttcagcg
     9241 ccagcgccgc cagttcgcgg tcggcaatgc cgccttctgt catcacgcga tccaccgcga
     9301 ggttaagctg ctgttcaatc tgggcgacgc tcagttcggg caaatcggta tcgccccggc
     9361 gtcggctctc ttgcagggcg tgggcggcgt cgatcgcctt ttcgccccct ttcacggcaa
     9421 cgtacatcag cacacctcca catgagtggt tcgcgggata gccagcaggc gctcgccgca
     9481 ggtcaggatt aggtcgatgc cgagcgggaa cgggtgcggg cgctcggtga gttcgtgcag
     9541 aatgcactct ggcagctgcg gagcgatcat tcgttcttcg gcaatgcccg cgccggtgag
     9601 acgcaacatg cgtccgccgc tcaggctggc gacctgtaaa atcagcgtcg cgcccgcttc
     9661 cggcgcaacg gcggtgtcgg tggaaagggc gttgagctgt tcgctggaaa tcgcctcatc
     9721 cgtcaccgcg aaggtcgcct gttccggctg gctgaccagc ggcgcgttgg tatgaaaacg
     9781 caggctctga ttgacgatat cgttacttaa tggcgtggca agccacaccg gcgtgtcgtt
     9841 atcggccagc gtcagcagca cgctggtggt ggcgatattc agcggctgcc agccgcgttt
     9901 gagctgatac agggcgacaa tcacgcccgg ctcgctcatg gcctttaaca ggcgacgaaa
     9961 actgtgctgg gcatcctgca cgggaagcat aaaagcggtt tccagggtca tgcgttgtct
    10021 ccgcgaacca tcgtaaagaa gtcgacccgg ctggcgttca cttcggcctg gcgtgcggca
    10081 atgcgtgcca tacggtcagc gtccagcggg gcaataaggg tttctgataa gttttgaaag
    10141 tggcgagatt gctgcatcag cgcatcaatc agcgcgcagc gttcggcgtg ctgtttatca
    10201 cgccccagca cccagctgta gccgagggtg ccgtcagtca ggcgtacggc ggcacgggtc
    10261 agcgtggcgt cgccggcaaa aaagcgttcg ccggtaccgc ccattcgcgc ctgaatctgc
    10321 accaggccag tttcggcagt gcggatcact tcatagtcgg cggtgatgtt cagcgcgttc
    10381 aggcgggctg ccagttcagc cggttggctg tgcgccagta cggacatcca gtgctggcgg
    10441 gtcgcggtat ctgcgtgcat tcagtgctcc atagtgaatt caatcatgtc ggcgcgcgtc
    10501 aggctgacgg agtactccgc cgggctgctt tcaccgtcac ggtggttaag ggtgcgcacg
    10561 cacagcagcg gcgacatatt cgggatttca aggcgctggc actctttggc ctgggcgcgg
    10621 cgggcgctga tccgcgtctg gctgcggcgc agcgcaattc cggtttgctc gcgcagaaaa
    10681 tcgtgcagcg aaccgctgtc gaaacgttgc agtgtcggcc agagggtgag gtccgcaaag
    10741 tagtggtcga ttaaacagag cgcgacgccg ttgacgcgac gcagggtgcg caaatggata
    10801 acgttctcac cctcggtaat ccccagcgcg tcagcaacgt gaccggacgc ggggcgcaat
    10861 accgacagca gtttttcgct ggtgggatgg ctgccctggt ccagcaggtt ctggctaaaa
    10921 cgcgcctggg cgttgagcgg gtaatcgaac gggcgcatca gcaccagcac gccgatgccc
    10981 tgacggcgct gtacccagcc tttttccacc agttggtcga tggcgcggcg cagggtgtgg
    11041 cggtttacct caaagcgcgc cgccagttgc tgctcggcgg gaagatagtc gccgcagcga
    11101 tagtgttgac gaagctcctg ctcaagtttt gcggctatct cctgatagcg tgttgggtag
    11161 ctggtcggat gtgtagacaa gtgcatagat atcaatgcct cacttatcag ataaagtgct
    11221 tacgcaaccg ttgagagagg aaatccagca ggctgaccgt gacgatgata agcaccatca
    11281 gggcgcaggt ttgttggaac tggaaaccgc gaatcgcttc ccacagggtg acgccgatcc
    11341 cgcctgcacc gaccatgccg acgacggtcg ccgagcggac gttggattcg aagcgataga
    11401 gggagtagga gatcagcagc ggcattacct gtggcagcac gccgtaaagg atctcttcca
    11461 gcttgttggc gccggtggcg cgaatgcctt ccaccgggcc gggttcaatc gcttccaccg
    11521 cttcggaaag cagtttcgag agcacgccgg tggtgtgaat aaatagcgcc agcacgccag
    11581 cgaacggtcc gagaccgacg gcgaccacga acagcatggc gaagaccatt tcgttaatgg
    11641 cgcggcaggc gtccatcagg cggcgaacgg gctggtaaac ccaccacggc accaggtttt
    11701 cggcgctcat caggccgaag gggatggaga gaaccactgc cagcgcggtg ccccagacgg
    11761 cgatttgcag cgtgacggcc atttcggtga ggtaatcctg ccactggctg aaatcgggcg
    11821 ggaagaagtc ggcggcgaag gtcgccatgt tgccgccgtc tttgatcagc gtaagcgggg
    11881 ccatttccgc gccctgccac gagacgacca acacggcgag aacaatggcc cagctcagaa
    11941 gcgagaacca gctgcgcttg ggtggggcga tggtgatggt ttgcatgttt ggctccgttt
    12001 aatgttttct ccctctccct gtgggagagg gccggggtga gggcatcagc gtgcgcattt
    12061 ccccctcacc ctaaccctct ccctcaaggg agaggggacc gatcgagcac attactgcac
    12121 cgctttactc accgaactca tcgcgcttaa cgcgttgttc aggcggtcca ggtcatccag
    12181 ctgcgcctga atctcggtgg ttttcgccag cttgtcctgc tcattcagtc cgttattgct
    12241 ttttacgccc tgaatctctt taaacagtgc gagctggcga atcggcacca gttgcaggtc
    12301 gctggaggcg cgaaacggtg cccagcccag gcgttccagc accgctttct cttccggcgt
    12361 ttttccgtag ttcataaaga agtcgtagat cttgtctttg gtggtttcgg agagattctt
    12421 gcgccagacg atcggatcgc ctgggatcag cggcgacttc cagatcacct tcagttcttt
    12481 cagcttctct ggcgcggagg ttttcagctt gtcgaggttt tcggtgttgt tggtggcaac
    12541 atccacctgc ttgttggcga cggccagcgc gttggtttca tgcccggcgt tgacggtgcg
    12601 cttaaagtcg ctggcggaga tattgttttt ggcaaagacg tagtaaccgg ggacgaggaa
    12661 gccagaggtg gagttaggat cgccattgcc gaaggtgaga tctttccgct tcgccagcag
    12721 atcgttcagg ttgttgatcg gactatcttt gttgacgatc aacacgctcc agtaacctgg
    12781 cgatccatcc gccgcgaccg tctgggcgaa gacctggccg ttggcgcgat ccaccgcttc
    12841 cattgccgac aggttgccgt accaggcgat atccacttta ttgaagcgca ttccctggat
    12901 aatgcctgcg tagtctgggg caaagaaggc gttcaccttc acgcccagct tcttctccat
    12961 atcctgtaag aatggcgtcc attgcggttt caggttttgc tgtgattccg ttgaaataat
    13021 gccgaaattc agcgcctttt cctgctcttc ggcgtgtgcc gggcttaaca gggtgctgag
    13081 gctgaacatg ctggtgaagg ccagcgaggc aattatctta gcgttcattc gttttcctca
    13141 atgatgggga tgtcaggcag ctttcgcgtt ctcttcgatg cgattaatgc tgcggtagag
    13201 atggtcaaaa cgttcgttat caaactgttg gctgctgccg tcgtagaaaa cgtgcccctg
    13261 gcgcagggcg acgatgcgtt cgcagtagcg cagggcgtaa tccacctgat gcagcgtgac
    13321 gaccacggtg atgccgtcgt tctgattgat gtcgcgcagg gtgtccatca cgatgccggc
    13381 ggattccggg tccagcgagg cgatgggttc atcggccaga atcaccttcg cctgctgcat
    13441 cagcgcgcgg gcaatcgcca cacgctgctg ctgtccgccg gagagggtgg aaacgcgttg
    13501 atgggcaaaa tgcaccatgc caacgcgggt cagcgcctgt aacgcgcgtt gtttctgctc
    13561 gcgggtaaac cagctaaaac aggtgcgcca gaacggcgtg ctgccgagtg cgccaatcag
    13621 cacgttctcc agtacgctca ggcggttcac caggttgaat tgttggaaga tgtagccggt
    13681 gttggcgcgg cttttgcgga tatcgcgcgc cagacggcct tcgcgctgga ctgtgcggcc
    13741 cagcagctcg atatggctgc cggcggattt atcgccggta atcaaaccgc ttaagtgacg
    13801 taaaagggtg gatttgccgg aacccgacgg cccaagcaga gccaccattt caccgtgatg
    13861 aatgttcaga tcaaccgcat gcagcgcctg atgctgattg aaggttttgg cgagcttctc
    13921 gacacggata atcgtttgca tgatgcagcc tccctaaaaa agtggctcca tcgtggcgga
    13981 ttattgtgac gatttggtta attaaaagtg actaacagat gaagagttaa cgggaattcg
    14041 atgacagtgc gggagggcgg gcctcccggc gggttattgc gttggttgtt gtttgacgac
    14101 attaatcatc cacggtacgc caaatttatc ggtgactttg ccaaagccat gcgcctagaa
    14161 agtttcctgc caggccattt cgatttttcc gttagcggca agattgtcaa accagcgttt
    14221 tccttcttcg acctgttgcg aatcgagcac cagcgtaaag ccggagtagc tggcttttcc
    14281 tgacggcatg gcatcgctca tcatgatgtc gcttccggca atgcgcacat tggcgtgggc
    14341 gatggcggta tcgggaaatt gcattccgga agggcagttc tcggcgctgt cctgcgctga
    14401 ttttggcatt tcgccgaagc tgattttata gagcagttca gcgcccaacg tacgttgata
    14461 ataagcaatt gcgtcggcac agttaccggc aaaagagagg tagggactta acggcatgat
    14521 ggtgacctca gttaagagaa gccaattaag tgtagttcga aatttataca gatgagaggc
    14581 tgcctgataa gacgcgcaag cgtcgcatca ggcagtcggc atcgttgccg gatgcggcgc
    14641 gagcgcctta tccggcttac cgatcacaat acaatcagtt ctttttcaca aactcagatt
    14701 tcagtttcat cggaccaaaa ccgtcgattt tgcaatcgat gttatggtcg ccttcaacca
    14761 ggcggatgtt tttcactttg gtgccaattt tcagcatcga agagctacct ttcaccttca
    14821 gatctttaat gatggtaacg ctgtcgccgt cagccagcag attgccgtta gcatctttaa
    14881 cgatcagctc gtcgctttcc tgtgcaggtt ctgcgtcgtt ccattcgtag gcacattccg
    14941 ggcagatgta catgccgtta tcttcgtaag tgtattcgga gttgcatttt gggcagtgtg
    15001 gtaatgacat gtggatttcc tcaaaagtca gcagggcaaa agcgccaaaa aacggcagat
    15061 tgccgaaaaa ggccgcaatt atacacaaaa tccctgagtt tgtcgggggt actgctgatt
    15121 ttagacgacg atcgttttca tttgaaagga aacgcaaaaa gaaaataatc tttttgc
//
LOCUS       DAZOZY010000094        15169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZOZY010000094 DAZOZY010000000
VERSION     DAZOZY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.094"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHURQ_002482"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHURQ_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR0824228.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHURQ_002483"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHURQ_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR0824229.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHURQ_002484"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHURQ_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR0824230.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHURQ_002485"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHURQ_002485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR0824231.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAECGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHURQ_002486"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHURQ_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR0824232.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHURQ_002487"
     CDS             complement(8214..9107)
                     /locus_tag="ACHURQ_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR0824233.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHURQ_002488"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHURQ_002488"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR0824234.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHURQ_002489"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHURQ_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR0824235.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHURQ_002490"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHURQ_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR0824236.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHURQ_002491"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHURQ_002491"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR0824237.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHURQ_002492"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHURQ_002492"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR0824238.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15169)
                     /locus_tag="ACHURQ_002493"
     CDS             complement(14275..>15169)
                     /locus_tag="ACHURQ_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR0824239.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3560 a         4283 c         3739 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgca ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaat
//
LOCUS       DAZOZY010000095        15102 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZOZY010000095 DAZOZY010000000
VERSION     DAZOZY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15102
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.095"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHURQ_002494"
     CDS             <1..1015
                     /locus_tag="ACHURQ_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0824240.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHURQ_002495"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHURQ_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR0824241.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHURQ_002496"
     CDS             1797..1904
                     /locus_tag="ACHURQ_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR0824242.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHURQ_002497"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHURQ_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR0824243.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHURQ_002498"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHURQ_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR0824244.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHURQ_002499"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHURQ_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR0824245.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHURQ_002500"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHURQ_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR0824246.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHURQ_002501"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHURQ_002501"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR0824247.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHURQ_002502"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHURQ_002502"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR0824248.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHURQ_002503"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHURQ_002503"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR0824249.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHURQ_002504"
     CDS             complement(11226..11813)
                     /locus_tag="ACHURQ_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR0824250.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHURQ_002505"
     CDS             11983..12561
                     /locus_tag="ACHURQ_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0824251.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHURQ_002506"
     CDS             complement(12691..13452)
                     /locus_tag="ACHURQ_002506"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0824252.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHURQ_002507"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHURQ_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR0824253.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
BASE COUNT         3678 a         4133 c         3792 g         3499 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag ataatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg ga
//
LOCUS       DAZOZY010000096        14886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZOZY010000096 DAZOZY010000000
VERSION     DAZOZY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.096"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHURQ_002508"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHURQ_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR0824254.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHURQ_002509"
     CDS             complement(1556..1879)
                     /locus_tag="ACHURQ_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR0824255.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHURQ_002510"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHURQ_002510"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR0824256.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHURQ_002511"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHURQ_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR0824257.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHURQ_002512"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHURQ_002512"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR0824258.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHURQ_002513"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHURQ_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR0824259.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHURQ_002514"
     CDS             7479..8516
                     /locus_tag="ACHURQ_002514"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR0824260.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHURQ_002515"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHURQ_002515"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR0824261.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHURQ_002516"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHURQ_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR0824262.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHURQ_002517"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHURQ_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR0824263.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHURQ_002518"
     CDS             10687..11568
                     /locus_tag="ACHURQ_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0824264.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHURQ_002519"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHURQ_002519"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR0824265.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHURQ_002520"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHURQ_002520"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR0824266.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHURQ_002521"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHURQ_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR0824267.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3589 a         3767 c         3899 g         3631 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZOZY010000097        14628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZOZY010000097 DAZOZY010000000
VERSION     DAZOZY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.097"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..877)
                     /gene="yhaJ"
                     /locus_tag="ACHURQ_002522"
                     /pseudo
     CDS             complement(1..877)
                     /gene="yhaJ"
                     /locus_tag="ACHURQ_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            982..1683
                     /locus_tag="ACHURQ_002523"
     CDS             982..1683
                     /locus_tag="ACHURQ_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR0824268.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1706..1870
                     /gene="yhaL"
                     /locus_tag="ACHURQ_002524"
     CDS             1706..1870
                     /gene="yhaL"
                     /locus_tag="ACHURQ_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR0824269.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2004..3314)
                     /gene="cyuA"
                     /locus_tag="ACHURQ_002525"
     CDS             complement(2004..3314)
                     /gene="cyuA"
                     /locus_tag="ACHURQ_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR0824270.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3342..4673)
                     /locus_tag="ACHURQ_002526"
     CDS             complement(3342..4673)
                     /locus_tag="ACHURQ_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0824271.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4948..6312)
                     /gene="tdcG"
                     /locus_tag="ACHURQ_002527"
     CDS             complement(4948..6312)
                     /gene="tdcG"
                     /locus_tag="ACHURQ_002527"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0824272.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6384..6773)
                     /locus_tag="ACHURQ_002528"
     CDS             complement(6384..6773)
                     /locus_tag="ACHURQ_002528"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR0824273.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6787..9081)
                     /gene="tdcE"
                     /locus_tag="ACHURQ_002529"
     CDS             complement(6787..9081)
                     /gene="tdcE"
                     /locus_tag="ACHURQ_002529"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR0824274.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9115..10323)
                     /gene="tdcD"
                     /locus_tag="ACHURQ_002530"
     CDS             complement(9115..10323)
                     /gene="tdcD"
                     /locus_tag="ACHURQ_002530"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR0824275.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10349..11680)
                     /gene="tdcC"
                     /locus_tag="ACHURQ_002531"
     CDS             complement(10349..11680)
                     /gene="tdcC"
                     /locus_tag="ACHURQ_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR0824276.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11702..12691)
                     /gene="tdcB"
                     /locus_tag="ACHURQ_002532"
     CDS             complement(11702..12691)
                     /gene="tdcB"
                     /locus_tag="ACHURQ_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR0824277.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12790..13728)
                     /gene="tdcA"
                     /locus_tag="ACHURQ_002533"
     CDS             complement(12790..13728)
                     /gene="tdcA"
                     /locus_tag="ACHURQ_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR0824278.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14043..14261
                     /gene="tdcR"
                     /locus_tag="ACHURQ_002534"
     CDS             14043..14261
                     /gene="tdcR"
                     /locus_tag="ACHURQ_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR0824279.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            14517..>14628
                     /locus_tag="ACHURQ_002535"
     CDS             14517..>14628
                     /locus_tag="ACHURQ_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824280.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
BASE COUNT         3856 a         3883 c         3389 g         3500 t
ORIGIN      
        1 agtttgggaa tttcccgcag acaccaggat ttcgcttccc ccatactgtc acgacgccag
       61 gccataataa tatcgatctc actggtcgat tccgggctga cgacacgcaa ccgcccttcc
      121 gcaatatctt tttcgaccat cggatacggc atcgtcgcca cgccaagccc cgccagtaat
      181 gcctgacgtt tatcttcaat cgtgctcacc gttaagcgcg gctgtttgtc cagcagttgt
      241 acggtcaaca ccgggcgctc acgagcggta tccgctaccg caattccacg atatttcacg
      301 cgcgtcactt cagataacgg ttccggctcc tgatgaatcg ggtgatcagg cgcggcaacg
      361 tagacgttca ttaacgtata gagcttgcgc gagttgatct ccgacgagga acgaaaatgc
      421 atatccggcg cgataacaat atccgcccgc ccctgctcca gtcgttccta cgcccccgcc
      481 agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt gtcgattaac
      541 gggaaaaagg caggtgtcgg taccagcgct tcggtcacaa tggtgagatg cgtttcccaa
      601 ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag caaaacgcgc
      661 cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc cgagcggtca
      721 aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag tgcggaaggc
      781 acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgca tccataaccc
      841 gtagtgcttc cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg
      901 aacataccgg gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga
      961 agtaaagaga ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca
     1021 gactacggat ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa
     1081 ttgttaggct atgcctccct gcgtgtgctt aaccaggaag tgctggcccc aggtgccgcc
     1141 tttcagccgc gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca
     1201 gagtatcgcg acagcgaagg caatcatgtt caggccagcg ccggtgaggc gttgctgctc
     1261 tctacccagc cgggtgtcag ctacagcgaa cacaatctca gcaaagacaa accgttaaca
     1321 cgaatgcagc tttggctgga cgcctgcccg cagcgagaga atccgctgat tcaaaaactg
     1381 gcgcttaata tgggcaagca gcaattaatc gcctcgccag agggtacgat gggaagcctg
     1441 caattacgcc agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat
     1501 ttccagttgc atgggccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt
     1561 acgcatcatg aagagaaagc ggcgctgacc tgcggtgatg gggcgtttat tcgtgacgag
     1621 gctaacatta cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc
     1681 tagttgtttt tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gccagccagt
     1741 gaagcccgtg gtggcgaaag aacctgctcg caccgccaaa aattttggct atgaagagat
     1801 gttgagcgag ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc
     1861 taccgcgtaa agacaggttg ccggatgcgg cgaaaacgcc ttatccggcc tacaaaatcc
     1921 aacaacatta acgaattaaa caacccgtag gccggataag gtgcgccagc atcgcatccg
     1981 gcacaatact caaaacctgg cgtttatctg gccttgctcg ccataatctc gataatctgc
     2041 cgatccgttt gctgcatcga atggcttgct aacgcacaca ggttggcaat cgactgctca
     2101 acatcatgcg ccacgatccc ttcattgccg gtcacggcgg tatcatccag cgccattaac
     2161 accgctttcc acgcagccga agcactggtc gaaaccttca tcgcgcagct gttcgacgca
     2221 ccatcgcaaa tcatgccgct gacatcgccg atcatactgc tgatcgccat cgagatagtt
     2281 tcataacgcc catccaccag ccatgccatc ccggcggcgg cccccattgc tgcggtcgtt
     2341 gcggcacaaa gtgcagacaa acgcggtaac tggttatgga tgtaaattgc gctcaaatga
     2401 gaaagcatca gcgcacgcgc cagtcgttca tcatccgctc cgaagtgttc tgctacaacc
     2461 accacaggca ttgttgcggt gatcccctgg ttacccgagc cggagttact catagccgga
     2521 agcgtagcgc cgcccatacg cgcatcggat gccgcgctgg tacgaatcac aatggatgaa
     2581 gagagatctt tcgccagcaa accgcgctcg cactgttttt ccagcgtcgc gccaatatgc
     2641 agcccccact taccgctcaa accttcctgc gataacgcac aatttagctt cgcggaatcg
     2701 agaataaagc ggatcgccgc aaacgggact tcattgacga acttcaggat ctcagccagc
     2761 gtcgttctgg aaagcactgt cagcggagac tcttgctcgc cctctgccac acacgcctgc
     2821 tgggtaaaca ccacaccatt gtgcgtctca atatgcacaa tgttggtatg cccgccgacg
     2881 atggtgacac acgcccactt ctcaccgttc cagactttgg cgcgtgagaa gaggatttca
     2941 ttgcaaggtt cctggatctt aacggagact ttccccgccg ccagcagtgc tttggcatcg
     3001 gcaattgcct gcgcagttgc gtctttcagc acttccagcc cggcgttggc atttccacct
     3061 aacgccccca gcgccgccgc aatcggcagc cccaccattc ccgtgccggg aacggtgacg
     3121 cccagaccgt tcttcatcag atttggcgaa acccaggctt ctacacgttc aaccggacct
     3181 tccagttctg ccgcagcaac cgccgccgcc agcgccagtg aaatcggttc agtacatccc
     3241 agcgccggtt ttacttcctc ctgaacggcg aggatgtaac gctgccataa cggatttaaa
     3301 gtcgaatcaa acatattaaa aaccttaaaa tttcaggtaa atcaagaaaa tgccaggaac
     3361 ggagaaacac aaagcaacaa accagtgacg ataatcaggt acagagacat ccctttgtat
     3421 ttgtgcaatg ccggtacttt gtaaaccagc cacgccggga tcaggcaccc taccatgccg
     3481 aaaatcgggc tacagataga ggtgaagctc aacaccggtg cgttcagtac gatagcgctc
     3541 caggccagca aaatggcgaa aatcatgatg ccgcgctgaa caagattttc gttaatcttc
     3601 tcggcaggca tcttgcgacg caggatgttc attacgatcc cttgcgttgc ttcgcgaaag
     3661 cctaaataga cgccaaagaa tgcagtcatg acagcaaaga tattgagaat gacgctgacc
     3721 actttcaccc acgctgcgcc gtcaccgcta ataaactgtg cggcaatcgc cagcgcggaa
     3781 atattctgct cgtaggcttt taccgcttcg tcatgcccca tcgccagcgt gaacgacacg
     3841 gcgtagaaaa agacggtgac aaacaaaatg ccaaacgcga tattcattgc ccgcaatgct
     3901 ttatgccgcg ccacttcaat agatttttcc cgcgagcgat aagagatcac catcgggctt
     3961 aacgtctgga taaacaaaat cgacgtcagg gtaaacggca gcgtaataat ggcgtttttc
     4021 accagcagcc ccagcggcgg tagcgaaccg acgttataca gatgccacat tcccaccatc
     4081 gacacgccca gcgccgcgac caccagcagc ttggtcagca ccatgccggt cgaaattttg
     4141 aataacaatt tctcgccgcg tgaagagatc gccaccagaa tgcaaatcag taccagacca
     4201 taaaagggac tgtctgacag caacccttcc gtcacgccga aggtatgcag ataggaagca
     4261 ctatcgttgg tgatggcggt ggaatagacg aacatccaaa tcaccagcat tacgaaatag
     4321 aacgcaccta acaggatgcc ccagttttta cctaaataac cgctaatgac actcgggtaa
     4381 tctttacatt ctggtgattc tgccagcgta ttaataaaca accgctgaaa cagatacatt
     4441 gccgggtaac caatcaccga tgagagcaaa aatacccaca atcccatcaa accgacctgc
     4501 accgggagaa aaacaatccc cgcgccaatc gccatcccga tactcataat cacccagccg
     4561 gtgtcagtac tgtcgaattt gatcgcttct cgccactcgc tctcactcat tccagcacga
     4621 cccgccgggg tcgaagcgtc tgcaatgacg cctttattcg atgcaatttc cataatttct
     4681 cgctcaatat tttgtagggc ttattatttt tttccgagcc gcatcaaggc gatatgcggt
     4741 ctacgttttt gcaggcgagt tattagaata aagaaatgat acgcgcgagg tcagagaaaa
     4801 tcttcacaat caagactttg aaataacgga gatggataag aattttctac taaattaatc
     4861 gcaagagaat attcattctc tatttgcggc agatcacaaa aaaaaggtgc acatttgtgc
     4921 acccaaggat gaaagctgac agcaatgtca gccgcagacc actttaatgg ccagtcctcc
     4981 gcgtgatgtt tcgcggtatt tatcgttcat atctttgccg gtttcataca tcgtctcgat
     5041 cactttatcg agtgaaacac gcggtgccga ggtgcggcgc atcgccatcc acgcggcgtt
     5101 tactgctttc acggcattaa tggcattacg ttcaatgcac gggatttgta cctgtccggc
     5161 aaccggatcg caggtcagcc caaggttatg ctccatcgcg atttccgccg cattgcatac
     5221 ctgcgccgga ctgccgccca gtagttcagt taaccctgcc gccgccattg aacaggccac
     5281 gccaatctcc ccctgacagc cgacctccgc gccagagatg gaggcgttca ttttatacag
     5341 cgcgccaata gcccccgctg ccagaaaata gcgggcaatt gaccgctcgt ttaccggacg
     5401 acggaactta tcgtaataag ccagcactgc cggaataatg ccgcacgcac cgttagtcgg
     5461 tgccgttacc acgcgcccgc cagctgcgtt ttcttcacta accgccagcg cgtacatgtt
     5521 gatccagtcg atgacattca tcggatcgtt agagatgtta tcgctagaaa ccagctgacg
     5581 acgcagcgct acggcacgac gcggcacatt gagcggacca ggcagcacgc cttcagtgtt
     5641 catcccgcgt tcaataccgt catgcatcac ttgccagata cgggcaaatc ctgcgtcaat
     5701 ttccgctttg ctgcgcagcg ccagctcgtt gtgcatcatc aggccagata tagacaggcc
     5761 gttgtaatca cacattttca gcagttcacc tgctgagtgg aaatcgtaag gtacggacgt
     5821 ttcgacatcg tgcgacaggc cgaagtgttc ttcttcgaca ataaacccgc cgccgacaga
     5881 gtaataggtt ttacttaata gcgcttcctg tcccttccag gcagtgatcc gcattccgtt
     5941 ctcatggcga ggcaacattt cgggatggaa gataatgttc tttgctacag gaaaatcaac
     6001 aatatgcgca ccagatgcca ctggcagccg cccgctgcgc gttactaact ctataaatgc
     6061 agggatctca tcaatgacaa catcctgcgg actgtttcct gccagtccca tgataatggc
     6121 gacatccgtg gcatggcctt tgcccgtcag tgacaacgac ccatacagat cgaccacaat
     6181 atggctcgtc gcggttaata agccgctact ttccagccga tcaataaaac tttttccggc
     6241 attcattggc cccacggtat gcgaactgga gggaccaatc ccaattttga aaatatcgaa
     6301 tgcactaatc atatccacac cctcggattg ccgttcagtg aagtggagcg gaacgacctt
     6361 gcgaccgtcc cgctcacgag gctttacgca ctacgtactg cgatagcttc aatttccagc
     6421 ttcacatctt tcggcaaacg cgcaacctgc acacagctcc gggtcgggta ggtcgcctga
     6481 tgctcatcga agaactgctt atagacttca ttgatggtgg caaaatcatt cagatcggtg
     6541 atgaacacgg tcatcttgat gatatcgccc acgctcagcc cggcagcaac cacgatcgct
     6601 ttgacgtttt cgaggcttaa acgcgcctga tcttgcacat cagccgggat ctcaccggtc
     6661 tgcgggcaaa ccggaatttg cccggaggtg aagaccatgc tgcctaaatc aacgccctga
     6721 acataagggc cgattgcgcc tggggcacgt tgcgtttcga taatcttttt catacatcct
     6781 ccggcgtcag agcgcctggg taaaagtacg tgaaataaca tcctgttgct gttcacgggt
     6841 cagtgcgttg aagcgcacgg cgtagccaga gacacggatt gtcaggttag gatatttttc
     6901 cgggtgctcg atggcatcca gcagcatttc ccgattcatt acgttgacgt tgaggtgttg
     6961 accgccttcg acatccgctt cgtggtggaa atacccatcc agcaggccga caaggttggt
     7021 tttacgtact ggatcttctt tgcccagcgc cgcaggaacg attgagaagg tgtacgagat
     7081 cccatctttg gcgtaggtga acggcagttt cgccaccgac gtcaatgagg ccacggcacc
     7141 tttgcggtca cgaccatgca tcgggttagc gcccggcgcg aacggtgttc cggcgcgacg
     7201 accgtccggc gtattaccgg ttttctggcc gtacaccacg ttagaagtga tagtcagaat
     7261 cgactgggta gggacggcgt tgcgataggt tggcagcgct ttaattttct tcataaagcg
     7321 ttcaaccagg tcgcaggcaa tgctgtctac gcgctcgtcg ttgttgccgt actgcggata
     7381 ttcaccgtcg atttcaaagt ccaccgccag gccgttttcg tcacggattg gtttcacgcg
     7441 ggcatatttg atggcagaca gggagtccgt cgccaccgac aggcccgcga tgccgcatgc
     7501 catagtgcga tagacatcac gatcgtgcag cgccatcagc gaagcttcgt agctgtactt
     7561 gtcgtgcatg tagtgaatga tattcagcgc gctgatgtac tgcaccgcca gccagtccat
     7621 gaagtgatcg aggctgtcca tcactttgtc gtaatccagc acgtcgtcca tcagcggtgc
     7681 tgttttcggc ccgacctgaa tcttcagctt ctcgtccacc ccgccgttaa ttgcgtacag
     7741 cagtgttttc gccaggttag cgcgtgcacc aaagaactgc atttgcttac caatcaccat
     7801 tgggctgacg caacaagcaa ttgcgtaatc gtcgctgttg aagtcagtac gcatcagatc
     7861 gtcgttttca tactgcaagg aagaggtgac gatcgacacc tgcgcggcat attttttgaa
     7921 ggcaatcggt aattcttccg accaaagaat ggtcaggtta ggttccggtg ccggccccat
     7981 agtgtgcagg gtgtgcaaat agcggaagga gtttttggtc accagcgtac gaccgtccag
     8041 ccccatcccg ccgatcactt ccgtcgccca gattgggtcg ccggagaaca gcgaatcaaa
     8101 ttccggcgta cgcaagaagc gcaccatacg gatcttcata atgaagtgat cgatcagttc
     8161 ctgtgcctgc tgctcattga gtacgcctgc tttaaagtcg cgctcaatgt agatatcgag
     8221 gaacgacgcc gtgcgcccca gcgacatcgc gccgccgttc tgtgatttca ctgccgccag
     8281 ataagcgaag tagagccact gcaccgcttc ctgcgcattc tgcgccgggc gagagatatc
     8341 aaagccatat ttcgccgcca tttcctgaat ctgcaacagc gcatgacgat gctctgccag
     8401 ctcctcacgc agacggatgg tggcttccag atcttcgcct ttttccagac gggactggag
     8461 atcggcaaat tgcagttcgc gttcacgcac cagataactg atgccataca gcgctacgcg
     8521 gcgatagtca ccgataatgc gcccacggcc atagccatct ggtaaaccgg tcagcacgcc
     8581 agatttacgg cagcgcagca tatccggtga gtaaacatca aatacgccct ggttatgggt
     8641 tttacgcaga tcggtaaaca gatattcaaa ttcactgtcc atttcacggc cataggcgtg
     8701 gaatgaactt ttaatcatat taatgccacc gaacggatgc agcgcgcgct ttaatggcgc
     8761 atccgtttgc agaccaacaa ttttttccag cggctggtta atatatcccg catcatgagc
     8821 ggtaattgtg gtggcaatat tggtatcgaa atcaaccggc gcgtgggttg cgttttcgat
     8881 acggatgcct tccattactt tttcccacaa ttccgtggtg gcaggcgtcg cttcggcgag
     8941 gaaagattca tcgccttcat acggtgtata gttatgttga ataaaatcgc ggacattaat
     9001 ttcgtttttc cagtccgtac ctttaaagcc aagccatgcg tcggcgtaca gcttatcgct
     9061 ggtatcaata tctatcttca tgaaaaataa tctctctaca atacttcaac taaattatgc
     9121 aaattctgcg ggcgcgttaa ctttgcctaa atgaatggca tccaaagcaa tcattttttc
     9181 ttcgttagtc ggaataacgg cacaaatgac acgcgcattt tcactggaaa caattcgctc
     9241 tccaaaagag ttagagcgat tattcatttc tgtatcaatc actacgccta atacagccaa
     9301 atgttccatg accagacgac gaattaaact tgaattctct cctattccgc cggtgaatat
     9361 aattccatcc aggcgacgta atgaagctgc gtgtccggca atatgacggg caattcggtg
     9421 aacaaaggtt ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg ctttttccag
     9481 aacacgtaaa tccgaagaaa gaccggaaat acctaataat cccgactctt tattcactac
     9541 gcgttccagg tcacccaggc tctggttggt ttggctggcg acccaggaca tcgcaccaaa
     9601 gtcgacatcg ccactgcggg ttcccatcat caagccttcc agcggcgtca ttcccattga
     9661 agtatcaaca ctctgaccgt tgcgaaccgc gcagattgac gcgccattgc caagatgcgc
     9721 cacaaccagg ccggagtcat cttccgccag attcagcaga gaatgtgcgc gctgggaaac
     9781 atagcggtgc gacgtgccgt ggaaaccata acggcgtaca cctaactctt cataatattt
     9841 ccacggcagg ccgtataaat aagcttccgg agccatcgtc tggtggaaac tggtatcaaa
     9901 taccgccacc tgagttacgc ccggaaataa ttgctgcgcc gattcaatac cacttaaatt
     9961 ggcgtaatta tgcaggggtg ccagtggaga aacgcgacgg atattatcaa tgacttcatc
    10021 ggtaataatg gcggactcgg taaaaatact gccgccgtga gcgatgcggt ggccaattaa
    10081 ggccacactg tcatttaaat tccgtttttc cagttcaaat gcaattgcct tcaatgcacc
    10141 ttcgtagctg tggtgagcca gcggtgctgg ctctccccca tttacggata agaatgcatt
    10201 ttccgagtta ataccgtcgg caatacctga cattaatact tcacagtcgc tggcatcgag
    10261 cacggaaaac ttaatcgaag acgaaccaca gttaataacc aaaacaaccg gaaattcatt
    10321 catctctttt ctcatcctga gttacggatt aaaacagttt gtatacgatg ttcaggatgg
    10381 tcagcagacc aatcacggta acaaacacgt tatccagacg accacggtat ttcgccagag
    10441 acggcgcttt acggatggca tacatcggca acaggcacag cagggatgcg ataatcggtg
    10501 cgcccatggc ttcaatcagg tcgaggatgt tcgggttggc gtaggcaaca acccaggtgg
    10561 agcccatgat gaagatcatg ctgatagtgt tcagtttacc cagcgacact ttagttttgt
    10621 cgcctttata accaaacttc aggaccaggc cattcagacc ttccagcgtt cccagatagt
    10681 gaccgaagaa agatttgaag atagccacga gtgcgatgat ggaagccgca tattccagtg
    10741 taatcgcgaa cgttgttttg gtaccggtca tggacgcaaa gtggttagcc agataagaaa
    10801 gcactggaat attctgcgct ttggcttccg ccatgttggc cggagacagc gtaaataggc
    10861 agctaaaggc aaagaacatc accactgcaa ccatcagcat gctggcacga gaaatgattt
    10921 gggaacattt acgttcggtg aagtcgcgac cgaagtcttt ctcatactct tcacgtttag
    10981 aaaccacgaa ggaagagacg attggcgaga aattaaagga gaaaaccatg atggaaatcc
    11041 ccagccagac ggtgatcagg ataccgtcat gaccggttaa cgacagcgaa ccgaggtcaa
    11101 cctggtcgat aactgcagag ttccagtaag ggatcagcga cagagaaatc agcaccaggc
    11161 tggcgataaa cggccatacc aggtagctca tcactttaac catcagatcc ttaccaaacc
    11221 agatgacgaa agccatcagc agcagcagga acagcgccac aaagccgcga ttcagcggtg
    11281 caaagccgag ctggttttcc cagaacgtca taaaggtatt ggtaatagta acgccataaa
    11341 tccacagcag tgggcaaatc gcgaagaagt acaggaacgt gataaccacg ccgccagttt
    11401 taccaaaatg ctcttccacc gtttccgtaa tgttgccgga agggttagag ccagaaagac
    11461 atagacgcgc cagcgcccgg tggcaataaa atgcgatggg gtatgccaat accaacatca
    11521 gaagaatcgg gatcagtccg ccaaaacctg cgcggatagg gaagaacagc accccggcgc
    11581 cgattgccgt accaaacaag cctaacgtcc atgtggtatc tgatttacgc caggacgatt
    11641 gttttgtctg gctggataca atgctatctg aagtactcat atcctatcct caacgaatta
    11701 attaagcgtc aacgaaaccg gtgatttgag agacgcgaga aagatcgata ttgccgccgg
    11761 aaataatact gacggttttt ctgttttgaa tatattggtc taatttaccg cttaataatg
    11821 cagcacatgc cagagcgcct gcgccttcgg tgacgacttt attgcgctga attaaggcaa
    11881 tcatactgtt tctgatttcg tcttcgctga ccagcacgat gtcatcgact aattcacgaa
    11941 cgatttcgta agttaaatta cccgggcggg agacatcaca accatccgcc agggtgccgg
    12001 tagttcggtg cgtggttatt tctccggagt ggaaagaagc cgccatgccg tgaacgtttt
    12061 cagactgtac gccaataaca cgaatggtcg ggttaataga tttaattgcc accgcaatac
    12121 cagcaattaa accgccacca ccaattggca caatcacgtt atcgacatca tagagatctt
    12181 ccataatttc cagaccaatc gttccctggc cagcaatcac tttcggatca tcgtaaggtg
    12241 ggataaaaat acggccttcc atttcgacaa tttcgctcac tttagcgata gtgtcgttga
    12301 agttatcacc atgcagaacg acttctgcgg agtagtcgca cgttgccgct actttggatt
    12361 ttggcgcacc ttttggcatc accactttac cgtcgatacc cagcatcgcg caggagagcg
    12421 aaaccccttg cgcatggttg cccgcagaac aggccaccac gcctttgcgt ttttccgcat
    12481 cggtcagtga acttaattta ttaaatgcgc cacgaatttt aaatgaaccc gtacgctgca
    12541 tattttcaaa cttcaggaat atttcacctt tgcaacgttc actaaaatag ttggagcgag
    12601 gcatgcctgt tttataaatt cgcccagcca gtcgttgttt cgcttcaata atatcatcaa
    12661 tagcaatcgg cagatcgtat gtaatatgca ttataaaacc tcttcgcctg aattaaatat
    12721 gtaggtaacc gtaaccgaca cctgcaaaac agacaggtgg attatttatg tttaaataaa
    12781 aacaaataac taaccaactt ctattaattg ccttcgtcta cacccattat aagatgaata
    12841 ctctttggct aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca
    12901 taccgcggca tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa taaattgatt
    12961 agaaccaaaa ggtgacgtca tatcacaagg aattacagtt aagaaatcag cattgagaac
    13021 aagattataa attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt
    13081 tctttgtaac gtagtaagca gttcgctgta gtaccccata ttagtttgtg gcaacaccca
    13141 ctgttcgttc ttcaacgact ccagcgtggt ggtgccggtg catgttcggg acttactggc
    13201 taccagcaca aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc
    13261 tgcacttaac gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga
    13321 agacagttgc gcttcataca tagaaacctg cgctttcggg aacacctctt tgaacttgtt
    13381 gatcatccct gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac
    13441 caccgcctca gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat
    13501 ggattcggaa cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt
    13561 ccgcaccact aattccacac caaaataatc ttcaatatcg ttaatgattt tactgacggc
    13621 cggttgagtt aaccctaatt cttttgcagc cgagccgata gagccacttc taatgacttc
    13681 ctgaaagact accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt
    13741 taattacctc attgacggca tgaagtgtat caaaatgaaa tgaacaggat atgtgcgacc
    13801 actcacaaat taactttcaa tactttccag ggtatcgtca ttacaattaa atacctttaa
    13861 atatcaacaa gttaaagcat aaaaatcagc ataaaccctg atttttgtca aaaaaaatga
    13921 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    13981 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    14041 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    14101 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    14161 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    14221 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    14281 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctgatc gccgcgataa
    14341 ggtcagacaa agacaacaag ggaaattttc acagagcttt tgatcgtcgt aggccacaga
    14401 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgaaagaac
    14461 cctgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    14521 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    14581 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttg
//
LOCUS       DAZOZY010000098        14523 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZOZY010000098 DAZOZY010000000
VERSION     DAZOZY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.098"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHURQ_002536"
     CDS             <1..161
                     /locus_tag="ACHURQ_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0824281.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHURQ_002537"
     CDS             complement(199..1260)
                     /locus_tag="ACHURQ_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR0824282.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHURQ_002538"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHURQ_002538"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR0824283.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHURQ_002539"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHURQ_002539"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR0824284.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHURQ_002540"
     CDS             complement(3454..4155)
                     /locus_tag="ACHURQ_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR0824285.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHURQ_002541"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHURQ_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR0824286.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHURQ_002542"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHURQ_002542"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR0824287.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHURQ_002543"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHURQ_002543"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR0824288.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHURQ_002544"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHURQ_002544"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR0824289.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHURQ_002545"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHURQ_002545"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR0824290.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="ACHURQ_002546"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="ACHURQ_002546"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR0824291.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHURQ_002547"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHURQ_002547"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR0824292.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
BASE COUNT         3841 a         3707 c         3610 g         3365 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgcaggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cat
//
LOCUS       DAZOZY010000099        14386 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZOZY010000099 DAZOZY010000000
VERSION     DAZOZY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.099"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            270..1388
                     /locus_tag="ACHURQ_002548"
     CDS             270..1388
                     /locus_tag="ACHURQ_002548"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR0824293.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            1454..1702
                     /locus_tag="ACHURQ_002549"
                     /pseudo
     CDS             1454..1702
                     /locus_tag="ACHURQ_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            1767..2135
                     /locus_tag="ACHURQ_002550"
     CDS             1767..2135
                     /locus_tag="ACHURQ_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0824294.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2229..2882
                     /gene="nfsB"
                     /locus_tag="ACHURQ_002551"
     CDS             2229..2882
                     /gene="nfsB"
                     /locus_tag="ACHURQ_002551"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR0824295.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2990..4237
                     /gene="ybdG"
                     /locus_tag="ACHURQ_002552"
     CDS             2990..4237
                     /gene="ybdG"
                     /locus_tag="ACHURQ_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR0824296.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="ACHURQ_002553"
     CDS             complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="ACHURQ_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR0824297.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="ACHURQ_002554"
     CDS             complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="ACHURQ_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR0824298.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="ACHURQ_002555"
     CDS             complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="ACHURQ_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR0824299.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="ACHURQ_002556"
     CDS             complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="ACHURQ_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR0824300.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="ACHURQ_002557"
     CDS             complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="ACHURQ_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR0824301.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12072..12755
                     /gene="cusR"
                     /locus_tag="ACHURQ_002558"
     CDS             12072..12755
                     /gene="cusR"
                     /locus_tag="ACHURQ_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR0824302.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12745..14193
                     /gene="cusS"
                     /locus_tag="ACHURQ_002559"
     CDS             12745..14193
                     /gene="cusS"
                     /locus_tag="ACHURQ_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR0824303.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3457 a         3874 c         3556 g         3499 t
ORIGIN      
        1 caaaatggct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
       61 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccatcag
      121 ccagctctgc cataacttgc cacattgaaa aagtagccaa attgcttcag tcaccatcat
      181 caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg
      241 aatagaaaac gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac
      301 cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa
      361 gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa
      421 agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc
      481 aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc
      541 atctggaaat ttgcggcggt ggcacacacc cgtttcagaa atggcagcgt caggaggtat
      601 gcgataacga gcgctatcaa cgaacgctgg aaaactttgg ctatctcatc cagcaggcga
      661 ccgtttttgg tcagcatgtc catgttggct gcgccagtgg cgatgacgcc atttatttgc
      721 tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata
      781 tgcaaggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcccg
      841 ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtt
      901 tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc
      961 ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg
     1021 taaatatggc gggattaatt caggctaccg cccactggtt actgacagaa cgcccgttca
     1081 aacatcagga gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg
     1141 ggctggaagg cgtcattacc gatccgcaca ctggcgatcg tcgaccgcta acggaagaca
     1201 ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa aattggtgca tcgagcgcga
     1261 ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt
     1321 tcgtcgccga tggtggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg
     1381 atgactaagt agggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac
     1441 atttaacaat gttatgaaac accctttaga aaccttgact accgcagcag gcattttgct
     1501 gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca
     1561 aaaactggtg accatgttcc atctgatgga tcttagctag ctttacactt tattgttttg
     1621 tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg
     1681 ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt
     1741 attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac
     1801 gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg
     1861 tttttaaaat tggcggcaaa atttttatgc tgtcgtcgga gctgcgcggc gtccccttta
     1921 tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcagatatac ccgagcatta
     1981 agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct
     2041 ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg
     2101 ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc
     2161 tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg
     2221 gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg
     2281 ccagtaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc
     2341 catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag
     2401 cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg
     2461 cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg
     2521 ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg
     2581 ataaaggtcg caagttcttc gctgatatgc accgtaaaga tctgcatgat gatgcagagt
     2641 ggatggcaaa acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggctctgg
     2701 gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc
     2761 tgaaagagaa aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag
     2821 attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt
     2881 aattctctct tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata
     2941 accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt
     3001 aatatcccag gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat
     3061 gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt
     3121 actgcggacc ttcgaaaaac gtgccatcgc cagttcacgg ctttggttgc aaatcataac
     3181 ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca
     3241 ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca
     3301 gttgtggatc atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa
     3361 tctggcgcag aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat
     3421 taaactgatc ggcgcgatcc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc
     3481 gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa
     3541 agatccgatt cttggtctgg tggccggtat tcagctttcc gcgaacgata tgctgaaact
     3601 gggcgactgg ctggagatgc cgaaatacgg cgcagatggc gcggtgatcg atattgggtt
     3661 aaccaccgtc aaagtgcgta actgggacaa taccattacc actattccca cctggtctct
     3721 ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa
     3781 gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg
     3841 tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga
     3901 gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat
     3961 tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga
     4021 tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta
     4081 cgcgtttact aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga
     4141 tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa
     4201 tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa
     4261 tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc
     4321 gttttaaatg ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc
     4381 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcagagatag
     4441 tttccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct
     4501 gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag cgttgctacc
     4561 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc
     4621 agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacaccgcg acggctgacg
     4681 cgagtcaaaa acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     4741 gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca
     4801 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     4861 ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc
     4921 aggatgcgat acaccacctg atttaccgct tttggaatgc ttttatccgg atcgcgcgct
     4981 tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc
     5041 gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg
     5101 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     5161 ccgatgattg ccagcacttt aatcaacgca aaccagaact cggtttcgcc atataagcgc
     5221 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     5281 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     5341 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     5401 tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgcca
     5461 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtagcccag caatacagcc
     5521 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     5581 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     5641 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     5701 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     5761 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     5821 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     5881 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     5941 gcagaccggc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc
     6001 cgtgatacaa cgcctcatcc agcttctgct cactgaatgt ttgcggatta ttcaacgacg
     6061 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga
     6121 ccccggcaag ggcaataaaa ccggtgcccg ttgccacaga aagatgaaag cccatccacc
     6181 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     6241 cgcccacccg acggaatgcc agatacaaca gcacgaagat gatcatcagt gtcatcggca
     6301 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     6361 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca
     6421 ccgacaccat gtcacgatcg cgggcatcga tataaatcca gctcgtcggg cgcgcattct
     6481 cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga
     6541 tttgctgctt catcggcgtc aggatcggta gctggcgcag tgcctgcggg ctatcgcgcc
     6601 agctttgcgg ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa
     6661 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     6721 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct
     6781 cggctagagc agaagccacg cttggcaccg ttcgcgctac ttcttcaatt tgctcagcca
     6841 tcgcgtcgat atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc
     6901 cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     6961 gcagccgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     7021 actgatcctg cggcttaagc tggatggtcg tttcaaccat ctcaagcgga gcagaatcgg
     7081 tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa
     7141 ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg
     7201 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     7261 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     7321 gccagtgcag tactttcagc aatagaggat gataaacacg aatcaaaaag cgattgagcg
     7381 ggttactgct ttccggcgga attttgccac ggatccagta ccccatcagg atagggatca
     7441 ctacgatcgc cagcagcgcc gcgcccgcca tcgcataggt tttagtgaac gccaacgggc
     7501 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa
     7561 tcagcagact gataaatagc gccggaccca cttcaacaga tgcatcggtg atcacctgcc
     7621 agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     7681 tatgtgcatt ttcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     7741 tgccgcccaa cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa
     7801 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     7861 acagcgcaca gactaccgcc accacgataa actcttccag caacttgccg ctgaggttgt
     7921 cgatggcgcg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     7981 gcagactact tttcagcgtt tccagtttgt ccttcacgga ggcgatcact tctcgggcgt
     8041 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgcca ttcagttcgg
     8101 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     8161 gcacgccatt ttcactggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     8221 agccgctggc gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt
     8281 cctggtttga agcatccagc gcgcttttta cttcggctag actgatgcca tattgcgcca
     8341 ggcgctgggg atcgataacc acctgatact ctttcaccac gccgcccacc gacgccactt
     8401 ccgcaacgtc agggatggtt tttagctcat atttgagaaa ccagtcctgc aatgagcgta
     8461 aatcggccag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     8521 cacccgtggc atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     8581 ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa
     8641 tgacatacac ataagagtct ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     8701 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     8761 gaccgggata gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca
     8821 ctggcgtgtt aatgatggtc caggtgcccc agatgctcag aaacaacgcg cccatcagca
     8881 ccaaaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
     8941 atgcgcattg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
     9001 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagtg
     9061 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
     9121 gtcggcatca acggtaatca cccgctgttc gttgccggta tcaatcagcg cctgtgacgg
     9181 aatgagcagc atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt
     9241 tagcgcctcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
     9301 cacgccaggt agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac
     9361 ggtgagcgta aactgcgagg catctttcac cagccaggcg atagactccg ggatcgcagc
     9421 agtgacccac accgggtcca taccctgaat tttcgctacc acgttatctt tggcgatatt
     9481 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
     9541 gcgagtctgg attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc
     9601 cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
     9661 cagtaaatac tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt
     9721 gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc
     9781 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
     9841 ggcaaaagtc agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg
     9901 agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag
     9961 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg ggtacatcgg
    10021 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc
    10081 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    10141 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    10201 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    10261 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcata gtcatctccg gccagttcac
    10321 ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac
    10381 cacgccagtg gcgctaataa cctgcggttg tacttcgctc atggtttcat gatgatgttc
    10441 gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc
    10501 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg
    10561 gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg
    10621 gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga
    10681 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    10741 ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    10801 ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc
    10861 agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc
    10921 cacatcccgc tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg
    10981 ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc
    11041 gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    11101 aagcccgccg gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggc
    11161 agtttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    11221 tgacgtttag cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc
    11281 agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    11341 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagttgctg attgaaatag
    11401 ctttgcgcga cattagaaac cagcagaata tgcaccgcgc gctgagcttc ctcagtggct
    11461 aaataatttt gtcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgaggtca
    11521 aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta
    11581 ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    11641 cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    11701 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    11761 acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc
    11821 ggcacgggca ttgccgtacg ctgataatcc ggtgccaatg aacaaccggt tagcgcaagg
    11881 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    11941 taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    12001 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    12061 gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    12121 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    12181 accatctggc gatgaccggt gattatgacc tgataatcct cgatattatg ctgccggacg
    12241 tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt
    12301 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg
    12361 actatctggt gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc
    12421 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    12481 tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta
    12541 ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct
    12601 cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    12661 ggctacgcgg caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg
    12721 tgggctatat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg
    12781 gcaacccgtc tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca
    12841 tggatcatga tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    12901 gaaattagcg ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc
    12961 ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat
    13021 agccacggca aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    13081 gacgccatac ccgataaaga cgctcagggt ggcgaggtgt atctcctttc cggcccgacg
    13141 attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac
    13201 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctttcg
    13261 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatct
    13321 gtcatcagca tcctgatcgt ctttatcgtg ctattggcgg tacataaagg tcacgcgccg
    13381 atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt
    13441 gacccgcaga ccgagcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
    13501 gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat cgctcacgaa
    13561 attcgcacgc cgattaccaa tctcatcacg caaacggaaa tcgccctcag ccagtctcgc
    13621 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
    13681 aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa
    13741 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta
    13801 gcggaagatc gcggcgtgga gttgcgattt gttggcgaca aatgtcaggt tgcgggcgat
    13861 ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg ttatacgcca
    13921 accagagaga caattgtagt gcgctgtcag acggtcgatc accaggtgca agttaccgtc
    13981 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctac
    14041 cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg gcattgggct ggcgatagtg
    14101 aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc gcgggggaca
    14161 aggtttgtga ttatgctgcc ggagagagag tgattattaa gttcaaattc agggcgtggt
    14221 gcatcacctc ctggtattgt tgatcaacaa attagagaaa aacttattaa acatatgaaa
    14281 tatcgaaggc ttttattttt caaagtttca cgagatgttt gtatatgaaa cttagttaaa
    14341 tctgtgtttc ttccaattgt agatcacaat gccctttcta aatttt
//
LOCUS       DAZOZY010000100        14297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZOZY010000100 DAZOZY010000000
VERSION     DAZOZY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.100"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="ACHURQ_002560"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="ACHURQ_002560"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR0824304.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHURQ_002561"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHURQ_002561"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR0824305.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHURQ_002562"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHURQ_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR0824306.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="ACHURQ_002563"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="ACHURQ_002563"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR0824307.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="ACHURQ_002564"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="ACHURQ_002564"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR0824308.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHURQ_002565"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHURQ_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0824309.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHURQ_002566"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHURQ_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR0824310.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHURQ_002567"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHURQ_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR0824311.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="ACHURQ_002568"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="ACHURQ_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR0824312.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="ACHURQ_002569"
     CDS             complement(13978..>14297)
                     /locus_tag="ACHURQ_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR0824313.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAZOZY010000101        14217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZOZY010000101 DAZOZY010000000
VERSION     DAZOZY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.101"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..183)
                     /locus_tag="ACHURQ_002570"
     CDS             complement(<1..183)
                     /locus_tag="ACHURQ_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824314.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSA"
     gene            complement(225..762)
                     /locus_tag="ACHURQ_002571"
                     /pseudo
     CDS             complement(225..762)
                     /locus_tag="ACHURQ_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(1123..1224)
                     /locus_tag="ACHURQ_002572"
                     /pseudo
     CDS             complement(1123..1224)
                     /locus_tag="ACHURQ_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(1409..1618)
                     /locus_tag="ACHURQ_002573"
                     /pseudo
     CDS             complement(1409..1618)
                     /locus_tag="ACHURQ_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
     gene            complement(1678..3126)
                     /gene="uxaB"
                     /locus_tag="ACHURQ_002574"
     CDS             complement(1678..3126)
                     /gene="uxaB"
                     /locus_tag="ACHURQ_002574"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR0824315.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3353..4303)
                     /locus_tag="ACHURQ_002575"
     CDS             complement(3353..4303)
                     /locus_tag="ACHURQ_002575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR0824316.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4442..4801)
                     /locus_tag="ACHURQ_002576"
     CDS             complement(4442..4801)
                     /locus_tag="ACHURQ_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR0824317.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(4801..5727)
                     /gene="glsB"
                     /locus_tag="ACHURQ_002577"
     CDS             complement(4801..5727)
                     /gene="glsB"
                     /locus_tag="ACHURQ_002577"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR0824318.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(5791..7179)
                     /gene="sad"
                     /locus_tag="ACHURQ_002578"
     CDS             complement(5791..7179)
                     /gene="sad"
                     /locus_tag="ACHURQ_002578"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR0824319.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7280..8161
                     /locus_tag="ACHURQ_002579"
     CDS             7280..8161
                     /locus_tag="ACHURQ_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0824320.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8238..9353
                     /gene="yneK"
                     /locus_tag="ACHURQ_002580"
     CDS             8238..9353
                     /gene="yneK"
                     /locus_tag="ACHURQ_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR0824321.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            9503..10693
                     /gene="ydeA"
                     /locus_tag="ACHURQ_002581"
     CDS             9503..10693
                     /gene="ydeA"
                     /locus_tag="ACHURQ_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR0824322.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(10718..11383)
                     /gene="marC"
                     /locus_tag="ACHURQ_002582"
     CDS             complement(10718..11383)
                     /gene="marC"
                     /locus_tag="ACHURQ_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR0824323.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            11595..12029
                     /gene="marR"
                     /locus_tag="ACHURQ_002583"
     CDS             11595..12029
                     /gene="marR"
                     /locus_tag="ACHURQ_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR0824324.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            12049..12432
                     /gene="marA"
                     /locus_tag="ACHURQ_002584"
     CDS             12049..12432
                     /gene="marA"
                     /locus_tag="ACHURQ_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR0824325.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            12464..12682
                     /gene="marB"
                     /locus_tag="ACHURQ_002585"
     CDS             12464..12682
                     /gene="marB"
                     /locus_tag="ACHURQ_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR0824326.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(12739..14178)
                     /locus_tag="ACHURQ_002586"
     CDS             complement(12739..14178)
                     /locus_tag="ACHURQ_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0824327.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     SRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3748 a         3593 c         3204 g         3672 t
ORIGIN      
        1 ggcggataaa tcgacagatt cagcaagatc attgcttaaa aaccgtggat taaaataata
       61 ctctgcgtca acgggaaata ccgcagttgc taagccaagg gcaatggtaa gccgtgcaaa
      121 gcgaggcagt attaacacct gatgcatacg atatgcctta aatgcggcac gaaaggctgt
      181 cattattaat tccgattgag tttattatta attaggtcac tggcttactg catggttgcc
      241 ttaatacgcg gagttaatgc accgtaatca ttgatcgtct gaaaactgat atccccggtt
      301 gcagcatgcg ggatatccac cgatacttca ccttttggcg gcaccatggt gtttgggaga
      361 ttgctatttc ctgctttcat atttgttacg gtaatgaaat agggcgttgg attaataaga
      421 gtgagtttgc taccgctacg acgaaaccga agcattgcag gcgcttcttc aggtgccatt
      481 gccaggttcg tggggcgata aaacattttt atgcggctaa taattgccag ctgtaatgta
      541 ttctcattct tctggtcttt ttccatagca ggaatagctt taacattcac ccagaacagg
      601 ctttcacggt cgcctggtaa ctgatgatta gtagcgttga ttatccgcag cgtattccag
      661 tgcgacagct gcgttggcaa cggtgctatg gcaagcaaag agcaataaaa gtaacactgt
      721 cgccataaag gaaatagagt agggggtacg cgtagtttgc atctgatgca ttctccatgt
      781 gttaagagga taagcgggaa aaccgttaca tcagacaatc gattaatgtt gctaatacat
      841 tggcgagtcg ccgggtttcc agacttaatg cgtttgtaat aaatgttggt tctcttttaa
      901 taggaaaacc ttgctacaaa gtaaatattt taaataacag taagtttaac tactgttgtt
      961 tattgatagt ttagatatta tgtgattttt catagcgctc caaatacaaa ccactcctca
     1021 ttaataatga gattcacatt ggtgaattgc tcgggtgtgc gtagccatgc cacacacacc
     1081 ctgtttcaat ttcagatgac gtatagccac cttaagattg aattatagat attgaacttt
     1141 gaaggtcgca tcagcgtttg cagtaccagc tacggactgc ccgagagcaa tataacgagc
     1201 ctggaatggc agtatattcg tgccacgata acatctttaa atcacaagga cacctaatct
     1261 gaacgaataa cgactaccag aaaagcactt cgcatcactc agacatgatt taacataata
     1321 tacattatgc gcacaaacat aaaccaaggg agaatccagt cgttgggccg gtcatgattg
     1381 ctcattaaca acgaccaaac cccatatctc acttactacc gtattctcgg cttaaccgtc
     1441 catgcaacct caacacattg gtttcaactg ccgtcaccac gttctccgga aaatctgtcg
     1501 gtaatgaagt cttcacgtta tccagtgctg ccggaatcat tctggcaaag tcacttagga
     1561 tttcatgcat ctgcacttcc gggaatctca gcacctttgc tgtcgccaaa aaatgttgta
     1621 actaatatag tgaaataaga aacccggtca aaaccgggtt cagaagtaac ggtgttatta
     1681 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
     1741 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
     1801 caacacaatc gctaccagtt cctgggtacc gataacatgg tcacgatgct ggctccagag
     1861 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
     1921 ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
     1981 gccgtttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat
     2041 accgttgagc gcgatagaca gcagctgatg cttatacggg ttacggaaac gcccggtgac
     2101 tgcactggcg aaagattcca gttcatcacg aggcaaatcc agtaccggaa taatttcttc
     2161 gtagatggct ttttcaacga atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt
     2221 atccagccct gcctggaacg ccaccggcac cagcgcggtg tgcgcaccgt tgaggattgc
     2281 cactttgcgt tctttatacg gtttaatatc gtcaacaatc agcacgttga gcggatattt
     2341 gtccaggcgc agctcagtcg ctaaggattt cggcccctga atcacaaaca ggtaaaagtg
     2401 ttcagcggtg tcgaggaaac catcgtgata acccagttct tcttccagtt tagccacttc
     2461 atcgcgcgga taaccggtaa cgatacggtc caccagcgta gaacagaagc tgttagcctg
     2521 atccagccat tgaataaatg cttctggcag tgcccactct tgcgcatagc gcagcaccag
     2581 ttcacgcaac gcgtcgccat tgtagtcgat caactcacac gggatgatga tccaaccttt
     2641 atccagcgca ccgttgaaat ggctaaagcg ttcgaacagc agacgggtca gttttgccgg
     2701 atagcttatc gctggcgcgt catcgaattt atcgcccgca tggtagctga tccccgcttc
     2761 ggtggtgtta gagaaaacaa agcgcatttc cgggttgtgc gccagtttca ggaattcatc
     2821 gtattcactg tagacgctga tttcacgatt aaccgagcga atcagacgcg cgtcgctgac
     2881 cgcttccccc ttctcattca ggccacggat aatggtggta tacagaccat cctgcgtgct
     2941 cagtgacggc gggaatgaag tttcaatcgg acgaacaacg accacgccag aattcagatc
     3001 ggtgtgctca ttcaggagat cgatttgcca gtcaacaaag gcgcgcagga agttaccttc
     3061 accaaactga atgatacgtt ctggatactg tgcaccggga aaatcgcgac gatttagtgt
     3121 tttcacaatg ggttcccttc tgattagtca tacaacctgt ttgaattggt acgacaggtt
     3181 agcaaacttt aatacgccga acccctgttt tgatcaactc ctgatgatta atgagcagtt
     3241 ttatgagaaa agtgtggcgc ggatcatggt ttaatcgagg aaatacgcct tttcctggat
     3301 cataaagtgg tagaacacat tgcattcaaa tcgcgcggaa tgaataaaga tgtcagacaa
     3361 cttcctcacc gtaacgcata gtgctggtac ggttgcgccc atctttcttc gagcgataca
     3421 gacacgtatc ggcttcgacc atcaatttat taaaatcatc ggtcagcgtt cggtacgatg
     3481 cgcgaccact accgacgcca atacttaccg tgagataaag cgttttttgt tgccaggtga
     3541 atggttgcag ttcaacgcct ttacggattt tttccgccat tagcagacca tcgacaggat
     3601 tcaccgacgg cactgcaaca gcaaattctt cgccgcccat gcgcgccacc agccccttat
     3661 caccgacaat cttctgaata tgccgggcaa acacgcttaa cactttatcg ccacattcat
     3721 gtccatagtt atcgttaatg cttttgaaat agtcgatatc aagcagcatg accgtcagag
     3781 gcacgatcaa cacgcccgtt gagaattgct cactgaacca gtccgcaagc aaaggccaga
     3841 aatccagact atcaatactg accgaaccaa tcgccccgac aatagcgcat aataatgcgg
     3901 caatcagaca gtaattaaat agccgtaagg cacttactgg ctcatactta tttttcccca
     3961 gacgcttatc ccgcacgacc agtaaggcga cggtaacaat aaacatcata ttggagaaat
     4021 tgataaccag tgaaaccagt ccccattcgg tggttatggc atcataaacc agcatcgcca
     4081 cataactgat cgcatagtaa tgcagtcgat taagccagac atagcgggca aacccccccg
     4141 ccattacgcc attcagcggc cagaaaagag aaagttcgtg aaccagccgt aactctgcac
     4201 caataaaata gaacagcgtg gttagcgcaa aaatagcgat gctattacgt agcagatgac
     4261 cttcctggaa caaacggaaa gttgaaatgg ggtgtacatg cattaattat ctgtccatac
     4321 aattgcgggt ggcaattatc tcaacgtaat aagtaaattc tcataaagga tagcatgttc
     4381 gctaacagcg gcggcaaata aaggtaccct gggaatgcta ttttatcgtt ttccgcaatt
     4441 ctcatggctg attcatctgt acaaccagcc agtgatagat taccgccacg atataacgtt
     4501 gttgttcttc gcttaacgaa acaccctgcg gaatgttatg ccacttagcc agacaaccgc
     4561 gacagcaggt cgcggtggcg tgctgagcga taaacaccgg atgcccgcgc atgggggttt
     4621 gcttaccgtc attagccggt aatgccggag caagacgttt agcgacaaaa tccgccgcat
     4681 gttgttcgat gactggcgcg cctttctcca ggcaatactg acgctctttc atgcccagac
     4741 gaaagcgaga gcgaaatttt gaacgggata aacgcgcgaa gagtggatcg agagactgca
     4801 ttaataaacc gaacgcccta actgtttcgt caattgttca aggacggcga tacccgcaag
     4861 cgagttacct gcatcatcca gttccggact ccagactgcg atggccattt catgcggaac
     4921 aatcgccaca ataccgccac caacgccaga tttcgccggt agtcccaccc gccaggcaaa
     4981 ctcccccgcg ttctggtaca taccactggt cgccatcagc gcgttaattt gccgcgcctg
     5041 cattggcgtc actacaggtt catcaatatg aatagctttc ccctgattag ccagaaagac
     5101 aaacgtccga gccagctcta cacagctcat tttcagagcg cagtaatgaa agtagttttg
     5161 cagaacggtt gtcacgtcat gatggaaatt gccaaacgac ttcatcagcc aggcgatagc
     5221 cgcatttcgc gcggaatgtt caaattcgga acgcgctacc accgtatcgt atgaaatatc
     5281 agacacaccg cttaagccgc gcacgacttc cagcatacgt tgccgtggtg cgcttaatcg
     5341 cccttgcaac atatcgcaga ccaccagcgc accagcatta ataaacggat tacgcggaat
     5401 accctgttcc atttccagtt gcactaaaga attgaacggt gatccagacg gatctttgcc
     5461 gacgcgttgc cagatttcct cttcggagta atgacgcatg gcgacaacga gactcagcac
     5521 tttggaaata gactgaatgg aaaaacgttc ttgcgcgtct ccggcctgaa aaagctgtcc
     5581 gtcaacggta cagatagcaa tgcccaatcg ggaaccgtct actgtagcca gcgccggaat
     5641 ataatccgcg actttaccct gaccaatgag cggccgcact tgccgcaaga tgttttctaa
     5701 aattgcatta tccatggcga ctgccacttt ctactcctgg accgcaggtc tgaaaagacc
     5761 tgcgagtata tcagagctga atatgtcgcg tcagatccgg tctttccaca ccgtctggat
     5821 attacagaat tcgtgtaagc cgaaatggga aagctcacga ccaaagccac tctttttcac
     5881 gccaccaaag gccactcgcg cgtcgctggc acaataacca ttgataaaca ccccaccgca
     5941 ttccagacgt gccgccatct gtctggcctg tgtttcgtcg gtggtaaaaa tggtcgctga
     6001 aaggccgaac tcactatcat tagccagttc cagcgcatgt tctgcatctt tcgcaacggt
     6061 gattgccgca acggggccaa acatttcttc ccgaaacgcg gtcatttctg gggtaacatt
     6121 cgccagaacc gttggcggat agtagttacc tgccccagcc atcttttccc cgcccagtaa
     6181 caaacgcgca ccctgcgcca gggttttctc tacctgatga tgcagctcat cacgtaaatc
     6241 aaaacgagcc attggtccga gagtgttctc ttcgtcacgg ggatcgccca ttttcaaggc
     6301 tgccgcagct gccacaaaac gttcggtaaa tgcagaagca attccctctt cgataataaa
     6361 gcgttttgcc gctgcacata cctgtccggt attctgataa cgtccggcta ccgccgcttt
     6421 aaccgccagt tccagatcgg catcgttaag cacaataaac ggatccgaac cgcccagttc
     6481 cagtacgcat tttttcagtg ccgctccagc ctgtgcgcca atagccgctc cagcacgaac
     6541 acttccggtc accgtgacag cagcaatgcg cgagtcttta atcatctgac tgacaccgtc
     6601 gttgtcggca ttcagccagc catatacgcc ttgcgggatt cccgcatctt taaacacctg
     6661 ggcaatgagc tgagcacatc ccatcacatt cggcgcatgt ttaagtaagt agccgttacc
     6721 tgcaagaatg atgggaacag cgccacgcat cacctgccat aacggaaaat tccacggcat
     6781 aatcgccaga atcgtcccca acggtcgata ctcaataacc gcctgctgat tttccaccag
     6841 tgtaggttcc gccttcagca ttgccggacc atgttctgca taccagtcac acaaattcgc
     6901 cgatttcgcc acttcagcgc gcgcctggtt gattggtttg cccatttcgc gggtgatcat
     6961 ttgcgccatt tcttcgctac gggcgcgcag agccttaccg atatcacgca ttttttcagc
     7021 acgataatct atatttgtct cgcgccagtc gcgaaagcct gctgccgcca gctcaagtgc
     7081 gttttcgata tcgttagcgc cagcccacgg cagcacagaa agttgttcac ccgtggcagg
     7141 atttattgaa attgcatgag ttgccggagt aatggtcatc ggggtatctc ctttatgagt
     7201 catggtatga agatacgcag atttactctt gctttaaaat gaataatatt aagccactta
     7261 ttcacgaatc gagaatgtta tggatctgac ccaactggag atgttcaacg cggttgccga
     7321 agctggcagc attacccagg ctgcagcaaa agtgcatcgc gtaccgtcga atctcactac
     7381 ccgtctacgc cagctggaaa cagaactggg ggtagatctg tttattcgcg agaatcagcg
     7441 tttacgtctc tctcctgccg ggcataactt tttacgctat agccaacaaa ttctcgcgtt
     7501 agtggatgaa gcgcggagcg ttgtcgctgg cgatgagccg caaggtttat tttctcttgg
     7561 ttcgctggaa agcaccgcag cagtgcgcat tccggccacg ctggcggaat tcaaccatcg
     7621 ttatcccaaa attcagtttt cactttccac cggcccttcc ggcactatgc tggaaggagt
     7681 gctggaggga aaactgaatg cggcatttat tgatgggccc attaaccata ctgccattga
     7741 cgggataccg gtataccgcg aggagctgat gatcgtcacg ccacaaggtc atgcgccagt
     7801 aatccgcgcc agtcaggtta acggcagtaa catttatgcc ttccgcacca attgttcgta
     7861 tcgtcgccac ttcgagagct ggtttcatgc tgacggtgcc gctccgggaa ctattcacga
     7921 gatggagtcg tatcacggga tgttggcctg tgtggtcgca ggagcaggca ttgcgcttat
     7981 tccacgctct atgctggaaa gtatgccggg gcatcaccag gttgaagcgt ggccgttagc
     8041 tgagcaatgg cgttggttaa caacctggct ggtctggcgt cgcggtgcga aaacgcgtcc
     8101 gcttgaggca tttattcaac tactggatgt gtctgactcg gcaaaacaga gttaccaatg
     8161 aactattttg atagttcttg cggctaatat gctctatata gtgatgttcc gatgacttat
     8221 gactatatgg ggcaaatatg gttacgccag taagcatcag caattacata tctcttcctg
     8281 atgattttcc cgttcgtaat attgcaccac aggtaaaaga agttttaaag gactttattg
     8341 atgcgctaag tacaataata tgtgatgaag agtggcgcac gtctttaaac atcaactcag
     8401 ccacaaaaaa gatatttaat aaccttgata acttatcata tattcagagg acttctttcc
     8461 ggggtaacga cacgctatac aatgaaaagg ttcagtttaa actcacttat cccgtcaaga
     8521 atggaagaca caaagaaaat attgaatttc aggtagtaat aaatttaagc cctatttatt
     8581 tagataattt tcgccatgat ggagaaatta atattttttg cgctcccaac ccaaagcctg
     8641 tcactatggg gcgcgtattt cagaccggcg tcgagcgtgt gctctttctt tttctgaatg
     8701 attttattga acaatttcca atgttcaacc ctggcgttcc gatcaaaata gcgcatacac
     8761 cacatattga acccctgcct ccggatcacc ataccgctgc agattactta cgccagtttg
     8821 atttgcttgt cctgaatttt atctctcgcg gtaattttgt catactcccc cgattatgga
     8881 ataactctga ggttcacaga tggttcgtca ataaagatcc taacttgatc accgccattc
     8941 tcgacataac ggacagcgaa ttaaaagagg atttattgca aagcctgatg gattcattag
     9001 gttctaacaa acatgtacta cccgaagtct gcatctgctt tttattcctt ttagcagaac
     9061 aagaatctcc tcattttcag gacttgtttt tatttttcgc caatatgtta ttgcactatc
     9121 accaatttat gaatcccaat gaaagtgatt tgaatgacgt gttgatgcca gcatcattaa
     9181 gtgatgataa aattatcaag catatggcgc gcaggaccct cgaactgttt gtaaaaaatg
     9241 aaacaccgcc aaaagttact cacgaagacc tggtgaaaaa caggcctcgc tcccctgtca
     9301 gaccacctat acccgcaacc tccaaaacgc cagacctccc tgaacgtcat taaaccgtga
     9361 tgttaccgac tctctgacgc ggaacagaat cagcgtcaga gaaacggaaa acgcgatcca
     9421 gatcacaaat acattgtatt cacatcatta accgttttaa gatcatttca tcactttttc
     9481 gcaactcacc cgataatctg ttatgacaac aaacactgtt tcccgcaaag tggcgtggct
     9541 acgggtcgtt acgctggcag tcgccgcctt catcttcaac accaccgaat ttgtccctgt
     9601 tggcctgctc tctgacattg cgcaaagttt tcacatgcaa accgctcagg tcggcatcat
     9661 gttgaccatt tacgcatggg tagtagcgct aatgtcattg ccttttatgt taatgaccag
     9721 tcaggttgaa cggcgcaaat tactgatctg cctgtttgtg gtgtttattg ccagccacgt
     9781 actgtcgttt ttatcgtgga gctttaccgt tctggtgatc agtcgcattg gtgtggcttt
     9841 tgcacatgcg attttctggt cgattacggc gtctctggcg atccgtatgg ctccggccgg
     9901 gaagcgggca caggccttga gtttaattgc caccggtaca gcactggcga tggtcttagg
     9961 tttacctctc gggcgcattg tggggcagta tttcggttgg cgaatgacct tcttcgcgat
    10021 tggtattggg gcgcttatca cccttttgtg cctgattaag ttacttccct tactgcccag
    10081 tgagcattcc ggttcattga aaagcctccc gctattgttc cgccgcccgg cattgatgag
    10141 catttatttg ttaactgtgg tggttgtcac cgcccattac acggcataca gctatatcga
    10201 gccttttgtg caaaacattg cgggattcag cgccaacttt gccacggcat tactgttatt
    10261 actcggtggt gcgggcatta ttggcagcgt gattttcggt aaactgggta atcagtatgc
    10321 gtctgcgttg gtgagtacgg cgattgcgct gttgctggtg tgtctggcac tgctgctacc
    10381 tgcggcgaac agtgaaatac acctcggggt gctgagtatt ttctggggga tcgcgatgat
    10441 gatcatcggg cttggtatgc aggttaaagt gctggcgctg gcaccagatg ctaccgacgt
    10501 cgcgatggcg ctattctccg gcatatttaa tattggaatc ggggcgggtg cgttggtagg
    10561 taatcaggtg agtttgcact ggtcaatgtc gatgattggt tatgtgggcg cggtgcctgc
    10621 ttttgccgcg ttaatatggt caatcattat atttcgccgc tggccagtga cactcgaaga
    10681 acagacgcaa tagttgaaag gcccattcgg gcctttttta atggtacgtt ttaatgattt
    10741 ccaggatgcc gttaataata aactgcacac ccatacatac cagcaggaat cccatcagac
    10801 gggagatcgc ttcaatgcca cccttgccca ccagccgcat aattgcgccg gagctgcgta
    10861 ggcttcccca caaaataacc gccaccagga aaaagatcag cggcggcgca accatcagta
    10921 cccaatcagc gaaggttgaa ctctgacgca ctgtggacgc cgagctaata atcatcgcta
    10981 tggttcccgg accagcggta cttggcattg ctagcggcac aaaggcaata ttggcactgg
    11041 gttcatcttc cagctcttcc gacttgcttt tcgcctccgg tgaatcaatc gctttctgtt
    11101 gcggaaagag catccgaaaa ccgataaacg cgacgattaa gccgcctgca attcgcagac
    11161 cgggaatcga aatgccaaat gtatccatca ccagttgccc ggcgtaatac gccaccatca
    11221 tgatggcaaa tacgtacacc gaggccatca acgactgacg attacgttcg gcactgttca
    11281 tgttgcctgc caggccaaga aatagcgcga cagttgttaa tgggttagct aacggcagca
    11341 acaccaccag ccccaggcca attgctttaa acaaatctaa cattggtggt tgttatcctg
    11401 tgtatctggg ttatcagcga aaagtataag gggtaaacaa ggataaagtg tcactcttta
    11461 gctaaccttg catcgcattg accaaaactt gaaccgattt agcaaaacgt ggcatcggtc
    11521 aattcattca tttgacttat acttgcctgg gcaatattat cctctgcaac taattacttg
    11581 ccagggcaac taatgtgaaa agtaccagcg atctgttcaa tgaaattatt ccattgggtc
    11641 gcttaatcca tatggttaat cagaagaaag atcgtctgct taacgagtat ctgtctccgc
    11701 tggatattac cgcggcacag tttaaggtgc tctgctctat ccgctgcgcg gcgtgtatta
    11761 ctccggttga actgaaaaaa gtgttgtcgg tcgacctggg agcactgacc cgtatgctgg
    11821 atcgcctggt ctgtaaaggc tgggttgaaa ggttgccgaa cccgaatgat aagcgcggcg
    11881 tactggtaaa acttaccacc agcggcgcgg caatatgcga acaatgccat caattagttg
    11941 gccaggacct gcatcaagaa ttaacaaaaa acctgacggc ggacgaagtt gcaacacttg
    12001 agcatttgct taagaaagtc ctgccgtaaa caaaaaagag gtatgacgat gtccagacgc
    12061 aatactgacg ctattaccat tcatagcatt ttggactgga tcgaggacaa cctggaatcg
    12121 ccactgtcac tggagaaagt gtcagagcgt tcgggttact ccaaatggca cctgcaacgg
    12181 atgtttaaaa aagaaaccgg tcattcatta ggccaataca tccgcagccg taagatgacg
    12241 gaaatcgcgc aaaagctgaa ggaaagtaac gagccgatac tctatctggc agaacgatat
    12301 ggcttcgagt cgcaacaaac tctgacccga accttcaaaa attactttga tgttccgccg
    12361 cataaatacc ggatgaccaa tatgcagggc gaatcgcgct ttttacatcc attaaatcat
    12421 tacaatagct agttgataac gtgacaacgt cactgaggca atcatgaaac cactttcatc
    12481 cgcaatagca gctgcgctta ttctcttttc cgcgcagggc gttgcggaac aaaccacgca
    12541 gccagttgtt acttcttgtg ccaatgtcgt ggttgttccc ccatcgcagg aacaaccacc
    12601 gtttgattta aatcacatgg gtactggcag tgataagtcg gatgcgctcg gcgtgcccta
    12661 ttataatcaa cacgctatgt agttattgtc aggctctgat cacagagcct gacatctccg
    12721 taatatcttc ctcaagtctc aaatattttc gccattgttg gcgatgagat cccgatacca
    12781 gtaaaacgac gctttacgta tccgttccag cgttcccttc ccttcgttgt ctttatcgac
    12841 ataaatcatt ccgtagcgct ttttcatttc ccctgttccg gcagaaacca ggtcaatgca
    12901 gccccacggg gtgtagccaa ttaagtcaac accatcttca actacggctt ttttcatttc
    12961 ccgaatatgg gaagcaaagt aatcaatgcg atagtgatcg ttcaccgtgc cgtcagcttg
    13021 tctctggtca accgcaccaa atccattttc gacaataaac agcggcaact ggaaatgatc
    13081 ccagaaccag ttgagtgaat aacgtagccc tgccggatca atttgccatc cccattcgga
    13141 tgtatcgata taagggttgc tgaccaaatc tcgtgattca acataatcca gttgcgggtt
    13201 atcgggagaa aattgtgtcg taaaagacat gtagtagcta aaaccgataa agtcgacaca
    13261 tccactggca agaatcgcgt tatcttctgg tgtgatatcg aggttgaatc ctttcctggc
    13321 aaagtaattc agcatatgtt gcggataata tccacgagca tgaacatcag taaaccagta
    13381 acgacgatgc atcgctttcg tggccatcat catatcgttg ggtgcacacg tcagaggata
    13441 gatggggcac atggcgatca tacagccaat gttaaattca ggattgatcg attttccagt
    13501 ctgtaccgcc agggcactgg caactaactc gtaatgtacc gcctggtaca ttatttgttc
    13561 gcgctcattg atatcttcct ctggcgaata caagataccg gaattagtaa atggacacag
    13621 gctttcgctg aaattcacct gattattgat ttcgttaaac gtcatccagt actttacttt
    13681 ttctttatag cgcgtgaaga ccgttgatgc gaagcggata aaaaagtcga tcagcttacg
    13741 gtttcgccag ccaccatatt ttgtcaccag atgataaggc atctcaaaat gcgaaagcgt
    13801 caccacaggt tccattcctt gcttcaggca ttcatcgaac agatcatcat aaaattgtaa
    13861 accctcttca ttcggctcct gttcgtcacc ttgcggaaag attcgtgtcc aggcaatgga
    13921 agttcgaaag catttgaatc ccatctcggc aaataactga atatctgttt tatagcgatg
    13981 ataaaaatca attgcttcat gattgggata attaagcccg tcgataacgc cttctgtcac
    14041 ttcacgcggc accccgtgag cgccagcagt catcacatca gcgatactga tgccttttcc
    14101 tccttcattc cagccacctt ccaactgatg cgcggctacc gcgccacccc ataaaaaacc
    14161 ttttttaaat cctgacatac acaactcctt aactaaaata gattaccacc agacttc
//
LOCUS       DAZOZY010000102        13988 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZOZY010000102 DAZOZY010000000
VERSION     DAZOZY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.102"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..245)
                     /locus_tag="ACHURQ_002587"
     CDS             complement(<1..245)
                     /locus_tag="ACHURQ_002587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824328.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAVSMYG"
     gene            280..1005
                     /locus_tag="ACHURQ_002588"
     CDS             280..1005
                     /locus_tag="ACHURQ_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR0824329.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            1498..2244
                     /gene="mipA"
                     /locus_tag="ACHURQ_002589"
     CDS             1498..2244
                     /gene="mipA"
                     /locus_tag="ACHURQ_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR0824330.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2334..3188
                     /gene="yeaE"
                     /locus_tag="ACHURQ_002590"
     CDS             2334..3188
                     /gene="yeaE"
                     /locus_tag="ACHURQ_002590"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR0824331.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(3239..4123)
                     /locus_tag="ACHURQ_002591"
     CDS             complement(3239..4123)
                     /locus_tag="ACHURQ_002591"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR0824332.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4207..5202)
                     /gene="gapA"
                     /locus_tag="ACHURQ_002592"
     CDS             complement(4207..5202)
                     /gene="gapA"
                     /locus_tag="ACHURQ_002592"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0824333.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5544..5957
                     /gene="msrB"
                     /locus_tag="ACHURQ_002593"
     CDS             5544..5957
                     /gene="msrB"
                     /locus_tag="ACHURQ_002593"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR0824334.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5999..6271
                     /locus_tag="ACHURQ_002594"
     CDS             5999..6271
                     /locus_tag="ACHURQ_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR0824335.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(6432..7073)
                     /gene="pncA"
                     /locus_tag="ACHURQ_002595"
     CDS             complement(6432..7073)
                     /gene="pncA"
                     /locus_tag="ACHURQ_002595"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR0824336.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7084..8100)
                     /gene="ansA"
                     /locus_tag="ACHURQ_002596"
     CDS             complement(7084..8100)
                     /gene="ansA"
                     /locus_tag="ACHURQ_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR0824337.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(8267..10123)
                     /gene="sppA"
                     /locus_tag="ACHURQ_002597"
     CDS             complement(8267..10123)
                     /gene="sppA"
                     /locus_tag="ACHURQ_002597"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR0824338.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            10284..10835
                     /locus_tag="ACHURQ_002598"
     CDS             10284..10835
                     /locus_tag="ACHURQ_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR0824339.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10952..11995
                     /gene="selD"
                     /locus_tag="ACHURQ_002599"
     CDS             10952..11995
                     /gene="selD"
                     /locus_tag="ACHURQ_002599"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR0824340.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGHIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            12000..13961
                     /gene="topB"
                     /locus_tag="ACHURQ_002600"
     CDS             12000..13961
                     /gene="topB"
                     /locus_tag="ACHURQ_002600"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR0824341.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3592 a         3373 c         3633 g         3390 t
ORIGIN      
        1 aatccataca tagaaacagc ggagatagca ataccgaaaa ccatccacat atctataggg
       61 cttttatttg tcgatttgac cgtagtgtac tttttgacta agtccgtacc tgatagcact
      121 gttaaaatta atgataatat agcgagggca gtatttacat taaaatttag cttagcgaat
      181 aaaagcaaca ttagtaaaat aaaaataagt aacgctatga ttaaaaatat tcctttcttg
      241 tccataaata ccccgtcgta tttttaaaaa aggtatctta tggcaaggat aagtaaaaag
      301 gaatctgcta atcacaataa agtgatggaa ttaatacatt cagataagct cctaacacaa
      361 gaagataggg aattcatatt caccaattat aggggggatg ggataggggc tacaggtgcc
      421 ttcttcacac ctgaaatttt agcatgggat tttgtacttg atgctgactg tactggtgac
      481 tgtattgaac tatgtgctgg tatcggtcgc ctcagctact accagtactt aaggaataaa
      541 ccaacgcata ttacatgcgt ggaactcaat cctgagtacg taatgatcgg gaagagggta
      601 ctaccagagg cagaatggat cactggtaac gcactacagt actcaccaga ccgtttctat
      661 gatgttgcct atggtaatcc accctttggg aagatcagta cctcaaaagc gtatacaggc
      721 cgttataagg gtagtgaatt tgaatacaag gtgattgaac atgcaagtac ctttgcgtcc
      781 tatggtgcat ggatagtacc gcaaggttcc gcaggctttg tctacagtgg ggtacattgt
      841 tatgagcgta gagaatcatg taagtaccag aagtttgtga aggatactgg ctatgagcta
      901 catccaggtg taggcatcga tacaagtatc tacagggatg aatggaatgg tactaaggtg
      961 atatgcgaat cggtgttagt agatgatgac ggtacaaatc tgtaactaaa ttagttaaga
     1021 aatcataaag atagtagtac ttgcaaatgt tcccattgat gaagttatgc gtcctttcgt
     1081 tttttagcac aggttaagag ccgttcatat gaatagtaga ggctcccgga agaggtaaac
     1141 tgaacgacgt ctcttgctaa aacacagcaa ctctcatgcc attttatcga cgatgagaac
     1201 aacgtgcccg gtgatacacc ttcaaaatta agcgtagatg gcaaatggaa aacttgcctg
     1261 cacggctttc ttaatttcaa tggcatatca acaactcatc cataaatttt gcataattaa
     1321 tgtaaagaca aggctcgcca gtaacgctaa attcatttgg ctgtaagcgc ggtgtcatcc
     1381 gcgtcaggaa aattaaacag ttactttaaa aaatgaaaac gtaaaaaggt tgggtttcga
     1441 tgtattgacg ggtaaacttt gtcgcccgct aaacatttgt ttaaggaatg attaattgtg
     1501 accaaactca aacttctggc acttggagtg cttatcgcaa cgtctgcagg cgtagcgcac
     1561 gctgaaggta aattttccct gggcgcaggc gtcggtgtcg ttgaacaccc atataaagat
     1621 tacgataccg atgtttaccc agtaccggta atcaactatg aaggcgataa cttctggttc
     1681 cgtggcttag gtggtggtta ctacctgtgg aatgacgcaa cggacaaact ttcaattacc
     1741 gcttattggt cgccgcttta cttcaaagcg aaagacagtg gcgatcacca aatgcgtcac
     1801 ctggatgacc gtaagagcac catgatggct ggtctgtctt atgctcactt tacccagtac
     1861 ggttacctgc gtaccaccct ggctggcgat accctggata acagcaacgg cattgtctgg
     1921 gatatggcct ggttgtatcg ttacaccaac ggcggcctga cagtgacccc gggtatcggt
     1981 gtgcagtgga acagcgaaaa ccagaacgaa tactattatg gcgtatcgcg caaagagtcc
     2041 gctcgcagcg gtctgcgtgg ctataacccg aacgacagct ggagccctta cctggagctg
     2101 agcgccagct acaacttcct cggcgactgg agtgtttacg gtaccgcgcg ctatacccgt
     2161 ctgtctgatg aagttactga cagcccgatg gtggataaat cctggactgg cctgatttct
     2221 accgggatca cctacaaatt ctgataatgc atctttccag ggcattttgt gcataaaaac
     2281 agggcgtcac gccctgtttt gcattacggt ggtgcaatac ctgggaggaa acaatgcaac
     2341 aaaaaatgat tcaatttagt ggcgatgtct cactgccagc catagggcag ggaacatggt
     2401 atatgggcga agatgccagt cagcgcaaaa cagaagttgc tgcactacgc gcgggcattg
     2461 agctcggttt aaccctcatt gataccgccg aaatgtatgc cgatggcggt gccgaaaagg
     2521 tggttgggga agcattaacc ggtctgcgtg ataacgtctt tctcgtctct aaagtctatc
     2581 cgtggaatgc tggcgggcaa aaagcgataa atgcatgcga agccagttta cgccgtctca
     2641 atactgatta tctcgatctc tacttattac acaggtctgg cagtttcgct tttgaagaga
     2701 ctgttgcagc gatggaaaaa ttgatcgccc agggaaaaat ccgccgctgg ggcgtttcta
     2761 accttgatta tgctgatatg caggaactct ggcagctgcc ggggggaaat cagtgtgcca
     2821 ctaatcaggt gctttaccat ctcggttcac gagggattga gtacgatcta ctcccctggt
     2881 gccagcaaca gcagatgccg gtgatggctt acagtccatt agcccaggcc gggcggttgc
     2941 gcaatggact gttaaaaaac gcggtagtca acgaaattgc acatgctcac aatatcagct
     3001 cggcacaagt attgttggcg tgggtaatca gccatcaggg tgtgatggcg attccaaaag
     3061 cggccacgat tgcccatgtt caacagaatg cggctgtgct tgaggtcgaa ctttcgtcag
     3121 cggaattaac catgctggat aaagcgtatc cggcaccaaa aggaaaaact gcgctggata
     3181 tggtgtgatg ttgatacgtg ccggatgcat cgcacccggc acacaacatt aacgcaaatt
     3241 aacgtttcgc aacgcgaatg gattgcgcca gatgtgcagg tttctctttg gtgacttttt
     3301 gcgtttctga agcgtaagcc gtttctacac aaacaaatgt tttgtagcca tcatccggca
     3361 tatcgcccat gctaattgaa agcgccggtc ccgggttcca gcctacaacg ttcagatgat
     3421 gctggtggcc tacggcgata atacgattca gcgcttcatc attaatcacg ctgcaatctt
     3481 gcggattcag atacacgcga tcggtacggt cagggaaggt ctgaataccg tcggtcagta
     3541 catcttcttt cgcgtcattc actttatcaa tgaagcgatc gcccagccca ctgacgctta
     3601 ccttagcgat atcacccacg ttaaagtagg tatgcagggc agaggtggtt tcaaattcgc
     3661 catgtgattc aagatcgatt tcacaggttt tacccacgcg gaaatgcgct aacagcgtaa
     3721 agtcgtgcgg acagaatttt ttcgtctctt cgctttgcgt caattcaaaa gtcagcgcta
     3781 cgccatcagc atcttcatta tgtgatttca gcgtccacgg caggttgcgg gcaaaaccgt
     3841 gcgcaggcag accttgttgt gccgccggac caaaccacgg ccagcaaacc ggtacgccac
     3901 cgcgaatagc gacgccattt ttgaacggtg tgttgttgct caaccacaga acttcttctt
     3961 cacccgcagg tttccacgag agaaggtgtg cgccctgtaa tgcaaaagag gcttttactt
     4021 ggggatgatc gaccacaatg aggtccagtt catccagttt acgacgggag aggacagggg
     4081 agatttgttc gatgaccgga agggcaaaaa ttttcttaat catgacgcag tcctttaact
     4141 tcattttatc aggtaaaaaa aagagcgacc gaagtcgctc tttttagatc acagtttcat
     4201 ctcaacttat ttggagatgt gagcgatcag gtccagaact ttgttggagt aaccggtttc
     4261 gttgtcgtac caggatacca gtttcacgaa gttgtcgttc agagcgatac cagctttagc
     4321 atcgaacacg gaagtgcaaa cttcgccgtt gaaatcggta gatactacgt catcttcggt
     4381 gtagcccaga acgcctttca tttcgccttc agcagcggct ttaacggcag ctttgatctg
     4441 ctcgtaagtt gcagcttttt ccagacgaac ggtcaggtca actacggata cgttcggggt
     4501 cggaacgcgg aacgccatac cagtcagttt gccattcagt tctggcagta ctttacctac
     4561 agctttagca gcaccggtag aggacgggat gatgttctgg gaagcgccgc ggccgccgcg
     4621 ccagtctttg tgagacgggc catcaacggt tttctgagta gcggtagtag cgtgaacggt
     4681 ggtcatcaga ccttcgatga tgccgaagtt atcgttgata actttagcca gcggagccag
     4741 gcagttggtg gtgcaggaag cgttggaaac gatgtcttgg ccagcatatt tgtcgaagtt
     4801 agcgccttta acgaacatcg gagtgttgtc tttagacgga ccagtcataa ccactttctt
     4861 cgcaccagcg gtgatgtgtt tacgagcagt ttcgtcagtc aggaacagac cagttgcttc
     4921 agcgacaacg tcaacaccaa cttcgtccca tttcaggtta gccggatcac gttcagcggt
     4981 aacacggatt tttttaccgt taacgatcag atgaccgtct ttcacttcaa cggtaccgtc
     5041 gaaacggccg tgagtggagt catatttcag catgtatgcc atgtaatcag cgtctaacag
     5101 gtcgttgatt gcaacgatct cgatgtcaga acgtttctga gcagcacgga aaacaatgcg
     5161 accgatacgg ccaaaaccgt tgatacctac tttgatagtc atatattcca ccagctattt
     5221 gttagtgaat aaaaggttgc ctgtaaaatt acaaaaacct tacgcagcgt caagcggaat
     5281 cgtgtcaatc attgcgacaa atcaatcctg tgcctaagca ttacgcgact gactcgcctc
     5341 actcttcctt tgggcttgaa accacatggg gtcggcgccc cgaattttaa agggcaatta
     5401 cgataaaaat gtgattttca tcacgattta ggtgcagcca tttcgtcgag atcaagttta
     5461 ggacaaaatt ttgccagttg gtgttaatgt tttgttagaa tcagtcacgt aatgtgagca
     5521 cgattaaagt gagatgtgag caaatggcta ataaaccttc ggcagaagaa ctgaaaaaaa
     5581 atttgtccga gatgcagttt tacgtgacgc agaatcatgg gacagagccg ccatttacgg
     5641 gtcgtttact gcataacaag cgtgacggcg tatatcactg tttgatctgc gatgccccgc
     5701 tgtttcattc ccaaaccaag tatgattccg gctgtggttg gcccagtttc tacgaaccgg
     5761 tgagtgaaga atccattcgt tatatcaaag acttgtcaca tggaatgcag cgcatagaaa
     5821 ttcgttgcgg taactgtgat gcccatctgg ggcatgtctt ccctgacggg ccgcagccaa
     5881 cgggcgaacg ttattgtgtt aactctgctt ctttacgctt cactgatggc gaaaacggcg
     5941 aagaaatcaa cggttgaaaa aacgattcag caacttattc cacaggagcg gaaaaattat
     6001 gaatcttgat gacattatca acagcatgac gcctgaggta taccagcgtt tgtcgaccgc
     6061 cgttgaactg gggaaatggc ccgatggcgt tgcgttgacc gaggaacaaa aagcgaactg
     6121 cctgcaactg gtgatgctgt ggcaagctcg ccataatacc gaagcacagc atatgacaat
     6181 tgacacaaat ggtcagatgg tcatgaagag caaacagcag ttaaaagaag attttggtat
     6241 ctcagcaaag cctattgcta tgtttaagtg aatgagttat ctagtattta agcctgttat
     6301 taagggaggg caaatatact tgttaggacg tcgtgtttta gctgcaagta ggttaaatgc
     6361 aacactgaca agtattatct ggcctacaat gtcattgagg aaatgtaggc cagagatgaa
     6421 ttcaactaat attacccctg tgtctcttcc cagtctgcca gcgtatatag cgttgcccca
     6481 gctgctgaca tctccataaa cgcgtgcgca ctgtcctggg gctggatatt cacgccacga
     6541 caaccatcgg taatcacatt taccttataa cctaactgta acgcgtccag cacggtaaac
     6601 ttcacgcaat agtcagtagc caggcccata acgatcaatt catcgatttc atgatcgcgt
     6661 aaccagtcat cgagcgaggt tttctgccga cggccgttat caaaaaaggc actgtaactg
     6721 tcaactaaag gattttcgcc tttatggaac accgctgcga tcgctttttg gttcagtaac
     6781 ggatgtaatt gtgcgccttc actgttctgc acacagtgat ctggccagaa ggtttgtggc
     6841 aaaccgtcga gttggcctgg cgtataaggc tctacaccgt gctgactggc aaaactgccg
     6901 tgattcgccg ggtgccagtc ctgactggcg ataaccgctt caccgcgcga ctggcaccag
     6961 tcaatcaggc ggttagcgac atccaccgta ctgtcacctt ccggcacggc gagcgcgcca
     7021 ccagcacaga aatcattttg taaatcgacc agtaacaggg cgcgaggggg cattacagtc
     7081 tccttaatca tccggcgtca gttcgccgcg caggttttgg ctcatggcct tgcgaatggt
     7141 ttcagtatcc agttcctggc tcagcaggta atgcagtttg gttagcgtgg cttctacagt
     7201 catatctgca ccgccaataa cgccggcatg ggcgagggcg ttaccggtgg cataaccacc
     7261 catattcact ttaccggaca tacattgtgt caggttgacc accacaatac cgcgatcgct
     7321 ggcttcttgt aattcctgca ggaaggcttt gttttgtggc gcattaccca cgccatagga
     7381 gcgcagaatc aatgctttca ccggttggcg cagaaaattg cgcacgacgt cagcagaaat
     7441 ccctggataa atcgtcacta cgccaattgg ttgtggcgtg attggatgaa cgattaattc
     7501 cccttcaccg tgcggggcgg gtggcgtatt caaacgacga atatggatac cagcttccag
     7561 taacggagga aggtttggag aggcaaacgc atcaaaacca tcggcatggg ctttggtagt
     7621 gcggttgccg cgatacaatc ggttattgaa aaagagcgtt acttcgttga tcggataatt
     7681 cgccgcaacg tacaacgcat tcagcagatt aatttgtccg tcagagcgta actcagccag
     7741 cgggatttgt gaccctgtca caatgaccgg tttaccgaga ttctcgagca tgaacgacag
     7801 cgcagaggca gtatacgcca tcgtgtcggt gccgtgcagg atgacaaaac catcatagtc
     7861 gtcatagtgc gctttaatat cttcagcaat atgctgccag tcttctggcg tcatatcaga
     7921 agaatccatc agcggcgtat attcatgaat ggtgaaatct ggcatctccg ggcgatggaa
     7981 ttccggcatc agcgccagtt ggcgttgtag atgacctgac accggaatat aaccctgctc
     8041 ggaacgctgc atcccgatgg tcccgcccgt gtaggcgacg taaattgatt tcttttgcat
     8101 gatattgata tgaatgatat cgaaagagca aaagtatata cgtgaggcag gaggagcgca
     8161 cctgatacta tgcggataag gaaatatggg gcgagacagc gggaagagga ttcgtcgacg
     8221 taaaaaataa tgtggccgac gatctgtcgg ccactcagta caagacttaa cgcacgttgg
     8281 cgcaggtcag gcaaaacgca taacggtttt gtgggtcgtt aaacgcggcc agcttgtcgc
     8341 tttcactttt aacggtagag gctaccgagg caagcggtgc aggtaacatg gcctggaacg
     8401 catctggcaa cattgcccgg acagaaccag acatgttgtc catcactttg tcgaagaagg
     8461 tcggttcatc aacgtagtat tccagatgcc actgtttcac ttttgccagc tctgctgctt
     8521 tggcgaccgc atcatcgaaa tccccgagac tatcgaccag cccgttagct tttgcatcct
     8581 gaccggtcca gacgtggccc tgggcgattt tatcaatctg ctccggcgtc gaatgacgcg
     8641 catcagcaac cagcgtgata aagcgtttat agccattctc aatgcttaat tgcatcatct
     8701 gctgcgcttc cggcggcagt gccctggtga tagaaacatc cgccagcggt gaagttgaga
     8761 caccatcagt atgaacacca atcgaatcca gactattttc tacggtggtg atcacgccga
     8821 agataccgat agaaccggtc agggtgctgg ggttagccac aatgtaatta gctggcgtgg
     8881 aaatccagta accaccagat gccgccatgc cgcccatcga tacaaccaca ggcttacccg
     8941 ctgcccgggc tgctgccagt tcagcgcgaa tcacttcaga cgcggtaacg ctgccgcctg
     9001 ggctattaac acgcaggaca atcgctttca ctttcgggtc aaggcgagcg tcgcggattt
     9061 gtgccgcagt ggtatcaccg ccaacattcc cctgagtttc ctcgccatcc ataattgcgc
     9121 cattagcgaa gacgacaccg atgctgtcac cggtatctgc cggcgttttc aatgcgtaat
     9181 cgtaataact gatggcgcga taatttttat cagtcttact ccagccgaac tctttggtta
     9241 gagctttttc gatttcggca ctcgatgcca gtgcatcgac cagcttgttt tccagtgcat
     9301 atttcgcggt atcgccaccg gttttggtta aaccctcaag caacccttgc gcgccaggga
     9361 atacctgctg agcagggatc tgccggttag cggcaacagt attcagatag ttttgccaca
     9421 gctcaccaat ccagcggctg tcagcttcgc gggctgccgg tgacatgtca tcacgaataa
     9481 acggttcaac ggcagattta tacgtaccca cgcggaacac atgggtggaa actttcagct
     9541 tatccagcaa cgatttgtag tacagaccgt tggtagcaaa accgtgcaaa tccaccacgc
     9601 cttgcggtga cagccaaatt ttattggcga aactggcgag ataatattgc ccctggctgt
     9661 agttctcgcc aacggcataa accggtttcc cgctgtcacg aaactctttc agggctttgc
     9721 cgatgtactg catagacggc tggtcgccgc ctgcgaagtt tttcagatcc atcacgatac
     9781 cggtgatatt gcggtcgtcc ttcgcctggc gaatggtgtt gacgatatca aacagtgagt
     9841 tttcctgcag acgatcggaa ctggcaccaa gcagctggcg gcttaattta ctaaaccgct
     9901 gagaactgtc gggtttatcg acgatcacac cagaaatgtc cagcagcagt gcgccacgac
     9961 tggccgtttc tttcgaatca ccaccactga cctgcatcca gatccccaca ccaaccagta
    10021 cgaggaaaat aaagaacagg ttaagtacca tttcacggac gaaattcagc agacgccacg
    10081 tccatttaaa aaatccggca ataaatcgcc aaagggttcg catgtattct cccaacttaa
    10141 ggtcacacct gtcacaggcg caatagatgt ggttatcgta atgacccaac cggcacttgt
    10201 cagcaggaat tgcggactgt gctgtaacaa aatccactct cgtgttaatt ttgtgaataa
    10261 atatcacgac aggagttaat caaatggatg cactcgaact attgatcaat cgccgtagcg
    10321 cctcccgcct ggctgaaccc gcgccaacgg gtgaacaact gcaaaacatc ctgcgtgcgg
    10381 gtatgcgtgc gccggaccat aagtccatgc aaccgtggca tttttttgtg attgaagggg
    10441 aagggcgcga gcgtttcagc gccgtactgg aacagggggc gattgctgcc ggtagtgatg
    10501 acaaagctat cgacaaagcc cgtaatgcgc cgttccgcgc accgctcatc atcacggtgg
    10561 tggcgaaatg cgaagaaaat cataaagtcc cgcgatggga acaggaaatg tctgccggat
    10621 gcgcggtcat ggcgatgcaa atggcagccg ttgcccaggg atttggcggc atctggcgca
    10681 gtggcgcatt aactgaaagt ccggtagtgc gtgaagcatt cggttgccgt gagcaggata
    10741 aaattgtcgg ttttctctac ctcggcacgc cgcagttgaa agcatctacg tcgattaacg
    10801 tcccggaccc gacgccgttt gtaacttatt tctgataatt gtcgcgaaac tgtctggatt
    10861 gtgagcaaag gcgcaggaat tcagacactc tcacttatca cttcacggaa tgagggttac
    10921 catacccgga ttgcattgac aggagatgtc catgagcgag aactcgattc gtttgaccca
    10981 atacagccac ggagctggtt gcggctgtaa aatttcccca aaagtgttgg aaaccatcct
    11041 gcacagtgag caggcgaagt ttgttgatcc gaatttgctt gtgggtaatg agacccgcga
    11101 cgatgcggcg gtgtacgatc tgggcaatgg caccagcgtt atcagtacca ccgacttctt
    11161 tatgccgatc gttgataatc ctttcgattt tggccacatt gcggcgacta acgccatcag
    11221 cgatatcttc gcgatggggg gcaaaccgat tatggcgatt gcgatcctcg gctggccgat
    11281 taacaaactt tccccggaaa ttgcccgcga agtaaccgaa ggtggacgct atgcatgccg
    11341 tcaggcgggt attgcgctgg ctggcggtca ctccatcgat gcgccggagc cgatttttgg
    11401 tctggcggta acggggatcg taccgaccga gcgggtgaag aaaaacagta ccgcacaagc
    11461 cggatgcaaa ctgttcctga cgaaaccgct ggggatcggc gttcttacca cggctgagaa
    11521 aaaatcactg ctgaaaccag aacatcaggg actagcgacg gaagtgatgt gccggatgaa
    11581 catcgcaggc gcgtcctttg ccaacatcga aggcgtaaaa gcgatgaccg acgttacggg
    11641 ttttggtctg ctgggccatt tgagcgaaat gtgtcagggc gcaggtgtgc aggcgcgcgt
    11701 tgactatgac gcgatcccaa aattgccggg ggttgaagag tacattaagt tgggcgcagt
    11761 gcctggcggc accgaacgta actttgccag ctacggtcat ctgatgggtg aaatgccgcg
    11821 tgaagtgcgc gatttgctgt gcgatccgca aacttctggc ggtttgctgc tggcggtcat
    11881 gccggaagca gaaaatgagg tcaaagctac agccgccgag tttggcattg aactgacggc
    11941 aattggcgaa ctggtgccag cgcgcggcgg tcgtgccatg gttgagattc gttaattcaa
    12001 tgcggttgtt tattgccgaa aaaccgagtc tggcgcgcgc cattgctgat gtcctgccca
    12061 aaccgcaccg gaaaggcgat ggctttatcg agtgcggtaa tggtcaggtg gtgacctggt
    12121 gtatcggtca cctgcttgag caggcgcagc cagacgccta cgacagccgc tatgcgcgct
    12181 ggaatcttgc ggatttgccg attgtcccgg aaaagtggca attacagccc cgaccctccg
    12241 tgaccaaaca acttaacgtc atcaaacgct tcctgcatga agccagcgaa atcgttcacg
    12301 ccggggaccc ggatcgtgaa gggcaactgc tggtggatga agtgctggac tatctgcaac
    12361 tggcaccgga aaagcgccag caggtgcagc gttgcttgat aaacgacctg aacccgcagg
    12421 cggttgagcg ggcgctcgac cgtcttcggt ccaacagtga gtttgtgcca ttgtgcgttt
    12481 ctgcgctggc gcgagcgcgt gccgactggc tgtacggcat caatatgacc cgtgcgtata
    12541 ccattctcgg tcgcaatgcc ggttatcagg gcgtactttc cgtgggacgc gtgcagacac
    12601 ccgtgctcgg gctggtggtg cgccgcgatg aagagattga aaacttcgtg gcgaaagact
    12661 tctttgaagt caaagcacat atcgtgacac ctgccgatga gcggtttacc gctatctggc
    12721 aaccgagcga agcgtgtgaa ccgtaccagg atgaagaagg gcgcttgtta catcgtccac
    12781 tggcggagca tgtggttaac cgcattagtg gtcaaccagc aattgtcacc agctataacg
    12841 ataaacggga atcagaatcc gcgccgctgc ctttttcgct ttcggcgttg cagattgaag
    12901 cggcaaaacg ctttggtctg agcgcgcaga acgtgcttga tatctgccag aagctgtacg
    12961 aaacgcacaa gctaatcact tatccgcgtt ccgattgtcg ctatttgcca gaagaacatt
    13021 ttgccggacg ccacgcggtg atgaatgcca tcagcgttca tgcaccagat ctgttgctgc
    13081 agccagtggt agatccagat atacgcaacc gctgttggga tgacaaaaag gtcgatgcac
    13141 accacgccat catcccgacc gcacggagtt cagccatcaa cctgacggag aacgaagcga
    13201 aggtctataa cctgattgcc cgtcagtact tgatgcagtt ctgcccggat gtggtgttcc
    13261 gcaagtgtgt tatcgaactg gacattgcca aaggcaaatt tgtcgctaaa gcgcgttttc
    13321 ttgctgaagc aggctggcgc acgctgttag gcagcaaaga gcgcgatgaa gaaaacgacg
    13381 gtacgccatt gcctgtggtg gcgaaaggcg atgagttgct gtgtgaaaaa ggtgaagtgg
    13441 tagagcggca aacccagccg ccgcgccatt ttaccgatgc aacactgctt tcggcgatga
    13501 ccgggatcgc gcgctttgtg caggacaaag atctgaaaaa gatccttcgt gcgaccgatg
    13561 gtctggggac agaagcaacc cgtgccggga ttattgaact gctgttcaag cgtggttttc
    13621 tgaccaaaaa agggcgctat atccactcta ccgacgccgg aaaagcgtta ttccattcgc
    13681 tgccagaaat ggcgacgcga ccggacatga ccgcgcactg ggaatcggtg ctgacgcaaa
    13741 tcagcgaaaa gcagtgtcgc tatcaggact ttatgcagcc gctggtgggg acgctatatc
    13801 agcttattga tcaagccaaa cgtacgccgg tgcggcagtt tcgcggcatt gtggctccgg
    13861 gcagtggtgg cagtgctgat aagaaaaagg ctgcaccgcg taaacgtagt gcgaaaaaaa
    13921 gtccgccagc agatgaagtc ggaagcgggg cgatagcgta agcgagtgaa tctttcgtgc
    13981 tattcgag
//
LOCUS       DAZOZY010000103        13967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZOZY010000103 DAZOZY010000000
VERSION     DAZOZY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.103"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(2..94)
                     /locus_tag="ACHURQ_002601"
     tRNA            complement(2..94)
                     /locus_tag="ACHURQ_002601"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHURQ_002602"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="ACHURQ_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR0824342.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHURQ_002603"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="ACHURQ_002603"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR0824343.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHURQ_002604"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="ACHURQ_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR0824344.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4316..5377)
                     /gene="recA"
                     /locus_tag="ACHURQ_002605"
     CDS             complement(4316..5377)
                     /gene="recA"
                     /locus_tag="ACHURQ_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR0824345.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="ACHURQ_002606"
     CDS             complement(5457..5954)
                     /gene="pncC"
                     /locus_tag="ACHURQ_002606"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR0824346.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="ACHURQ_002607"
     CDS             complement(6099..7184)
                     /gene="mltB"
                     /locus_tag="ACHURQ_002607"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR0824347.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7440..8003
                     /gene="srlA"
                     /locus_tag="ACHURQ_002608"
                     /gene_synonym="gutA"
     CDS             7440..8003
                     /gene="srlA"
                     /locus_tag="ACHURQ_002608"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR0824348.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8000..8959
                     /gene="srlE"
                     /locus_tag="ACHURQ_002609"
                     /gene_synonym="gutE"
     CDS             8000..8959
                     /gene="srlE"
                     /locus_tag="ACHURQ_002609"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR0824349.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            8970..9341
                     /gene="srlB"
                     /locus_tag="ACHURQ_002610"
                     /gene_synonym="gutB"
     CDS             8970..9341
                     /gene="srlB"
                     /locus_tag="ACHURQ_002610"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR0824350.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9345..10113
                     /gene="srlD"
                     /locus_tag="ACHURQ_002611"
                     /pseudo
     CDS             9345..10113
                     /gene="srlD"
                     /locus_tag="ACHURQ_002611"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            10219..10578
                     /gene="gutM"
                     /locus_tag="ACHURQ_002612"
     CDS             10219..10578
                     /gene="gutM"
                     /locus_tag="ACHURQ_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR0824351.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10645..11418
                     /gene="srlR"
                     /locus_tag="ACHURQ_002613"
                     /gene_synonym="gutR"
     CDS             10645..11418
                     /gene="srlR"
                     /locus_tag="ACHURQ_002613"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR0824352.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11411..12376
                     /gene="gutQ"
                     /locus_tag="ACHURQ_002614"
     CDS             11411..12376
                     /gene="gutQ"
                     /locus_tag="ACHURQ_002614"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR0824353.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12373..13887)
                     /gene="norR"
                     /locus_tag="ACHURQ_002615"
     CDS             complement(12373..13887)
                     /gene="norR"
                     /locus_tag="ACHURQ_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR0824354.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
BASE COUNT         3255 a         3871 c         3488 g         3353 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatccct acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gagtgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcacca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata acctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgacccaggt
     4141 tttgctgata aacagattta atagtcaatg agtgtgcagc caattcgtta gaggatcgag
     4201 gtgagagtaa tttttcacgc cagtgttggc ctgttcccct caccctaacc ctctccccaa
     4261 aggggcgagg ggaccgtccg tgcacacatt atctattatc atcaataacg aacgattaaa
     4321 aatcttcgtt agtttctgct acgccttcgc tgtcatctac agagaaatcc ggcgttgagt
     4381 tcgggttgct cagcagcaac tcacgtactt tcttctcgat ctctttcgcg gtttccgggt
     4441 tatctttcag ccaggcagtt gcattcgctt taccctgacc gatcttctca cctttgtagc
     4501 tgtaccacgc gcctgctttc tcgatcagct tctcttttac gcccaggtca accagttcgc
     4561 cgtagaagtt gataccttcg ccgtagagga tctgaaattc agcctgttta aacggtgcag
     4621 cgattttgtt cttcaccact ttcacgcggg tttcgctacc caccacgttt tcgccctctt
     4681 tcaccgcgcc gatacgacgg atgtcgagac gaacagaggc gtagaatttc agcgcgttac
     4741 caccggtagt ggtttccggg ttaccgaaca tcacaccaat tttcatacgg atctggttga
     4801 tgaagatcag cagcgtgttg gactgcttca ggttacccgc cagcttacgc atcgcctggc
     4861 tcatcatacg tgccgcaagg cccatgtgag agtcgccgat ttcgccttcg atttccgctt
     4921 tcggcgtcag tgccgccacg gagtcaacga cgataacgtc tactgcgcca gaacgcgcca
     4981 gggcgtcaca gatttccagt gcctgctcgc cggtgtccgg ctgggagcac agcaggttat
     5041 cgatatcgac gcccagttta cgtgcgtaga ttgggtccag cgcgtgttca gcatcgataa
     5101 acgcacaggt tttaccttca cgctgcgctg cggcgatcac ctgcagcgtc agcgtggttt
     5161 taccggaaga ttccggtccg tagatttcga cgatacggcc catcggcaga ccacctgccc
     5221 caagcgcgat atccagtgaa agcgaaccgg tagagatggt ttccacatcc atggaacggt
     5281 cttcacccag gcgcatgatg gagcctttac caaattgttt ctcaatctgg cccagtgctg
     5341 ccgccaacgc tttctgtttg ttttcgtcga tagccatttt tactcctgtc atgccgggta
     5401 ataccggata gtcaatatgt tctgttgaag caattatact gtatactcat acagtatcaa
     5461 gtgttttgta gaaattgttg ccacaaggtc tgcaatgcat acgcagtagc ctgacgacgc
     5521 accgcatcac ggtcgccgct gaagcattcc cgccgggtaa tgccttcacc gcgggcagtg
     5581 gcaaaagcaa accagacggt gccgacaggc ttctcttcac tgccgccatc cggcccggcg
     5641 ataccactaa tagacacggc ataatcagca cgagccgctt tcagtgcacc tatcgccatt
     5701 tccaccacga cgggttcact caccgcgcca tgctgcgcca gcgtctcttc gcgtacgccg
     5761 atcatctgcg ctttggcttc gttactgtag gtgacaaatc cgcgttcaaa ccaggcggag
     5821 ctaccggcaa tatcggtaat cactttcgct acccaaccac cggtacaaga ctcggcagtt
     5881 gttacggttg cgccacgggc tttcagcgcc tgcccaacct gttcgcttaa ctgcatcagt
     5941 tcactgtcag tcatcacaac ctcagtcagt caaaaattta tgccggacaa gatagcactt
     6001 tcgtcaggaa gatggtgttg gcgttgcaat tttacgagga ggattcagaa aaaagctgat
     6061 tagccagagg gaagctcacg cccccctctt gcaaatagtt actgcacgcg cgccagcgcc
     6121 acggcttgtc ctaactgcca gaccgccatt gcgtaatggg tgctgtggtt ataacgggta
     6181 atggtgtaga agttcggcag accgtaccag tactggtaac cggtaccgac atccagacgc
     6241 agcaggctgg cttgttgatg gttgcccagc ggctgctgtg gcgttaaacc tgccgtggca
     6301 agctgcgaaa tgctgtacct ggttttgaag ccatttggca agcctggagc ctgaccgttt
     6361 gccattaccg cgacctgatc gcctttcacc cagccgtgcg ctttgaaata gttcgccaca
     6421 ctaccgatcg catcaaccgg atcccacagg ttgatatgcc cgtcgccgct gaaatctacc
     6481 gcatattgtt tgtaagacga cggcataaac tgtccgtagc ccatcgcccc ggcaaaggaa
     6541 cctttcagat tgagcggatc gtcctgctcg tcgcgcgcca tcaacaggaa ggtttccagc
     6601 tcgccagaga aatactccgc gcggcgtggg tagttaaatg acagcgttgc cagcgcatcg
     6661 aggatgcgag ttttccccat cacgcgcccc cagcgggttt caacgccgat aatcccgacg
     6721 ataatttccg gcggtacgcc atacacctgc cacgcgcgat tcaacgcatc ttcatactga
     6781 ttccagaaaa ccacaccgtt ctgcacgttg tccggcgtaa taaatttttt gcgataacgc
     6841 agccatgcgc cgttcggacc tgatggcggt ttcaccgatg tggttggtgc ctggttatcc
     6901 atcagccgca gtaccgaatc cagacgcttc gcctgggaga gaatttcctg caactgctga
     6961 cgatcgaaac cgtgtttgtt caccattctg tcgatgaact gctgggcatt cgggttatta
     7021 gcgaaatcgc cgcccatctg catcacattg tgctgaggct caagcaggaa gccgccagac
     7081 ggcgttccgg tggtcgtttc agtctcagta ggttttggct tgctgctaca ggcggcaagc
     7141 aacacaaaaa ggggaagcaa tgttacataa cgacgcttga acatgagggg tccatttaac
     7201 agattcaacc aggggcaagt atggtaaagc atcacgcccc gcacaaggaa gcggtagtca
     7261 ctgcccgata cggactttac ataactcaac tcattcccct cgctatcctt ttattcaaac
     7321 tttcaaatta aaatatttat ctttcatttt gcgatcaaaa taacactttt aaatctttca
     7381 atctgattag attaggttgc cgtttggtaa taaaacaata aatcctgaag gagagaacaa
     7441 tgatagaaac cattactcat ggtgcagagt ggtttatcgg gctgttccaa aagggcggag
     7501 aggtgtttac cgggatggtg accggcattc ttccgctgtt gattagcctg ctggttatca
     7561 tgaacgcact gattaatttt atcggtcagc atcgtattga acgttttgct caacgttgcg
     7621 ccggtaaccc tgtttcccgt tacctgctgc taccgtgcat tggcacgttt gtcttttgca
     7681 atccgatgac cttaagcctg ggtcgcttta tgccggaaaa gtacaaaccc agctactacg
     7741 cggcagcctc ttatagctgc cactcaatga atggcctctt tccccatatc aaccctggcg
     7801 aactgtttgt ttatcttggc attgccagcg gtctgacaac gctgaacctg ccacttggcc
     7861 cactggcggt gagctatctg ctggttggtc tggtcaccaa cttctttcgc ggctgggtga
     7921 ccgatctgac caccgccatt tttgagaaaa agatgggcat tcaacttgaa caaaaagttc
     7981 acctggcagg agcaacatca tgacgcgtat tcggatcgaa aaaggaacgg gtggctgggg
     8041 cggcccgctt gagctggaag ccacgccggg caaaaaaatc gtctatatca ccgccggtac
     8101 ccggcctgcg attgttgaca aactggcaca acttactggc tggcaggcta ttgacggatt
     8161 taaagaaggt gaacccgcgg aggcggaaat tggtgtcgcg gtaatcgact gtggcggcac
     8221 attacgctgc ggcatctatc cgaaacgacg tattcccacc attaatatcc actcgacggg
     8281 caagtccagt ccgctggcgc agtacattgt ggaagatatt tatgtctctg gcgtaaaaga
     8341 agaaaacatc actgtagtgg gtgatgcgac accacaaccc tcttccgtgg gccgtgacta
     8401 tgacaccagc aagaaaatca ccgaacaaag cgatggttta ctggcgaagg tgggaatggg
     8461 tatgggttct gccgttgccg tgttgtttca atctggtcgt gacaccatcg acactgtatt
     8521 aaaaaccatt ctgccgttta tggcgttcgt ctcggcgctc attggcatca ttatggcttc
     8581 tggccttggt gactggattg cccacggtct tgctccgctg gcgagccatc cactgggtct
     8641 ggtcatgctg gcgctcatct gctccttccc gctgctttca cctttcctcg gcccaggcgc
     8701 agttatcgca caggttatcg gcgtattgat tggcgtgcag attggtctcg gcaatattcc
     8761 gccgcatctg gctttaccgg cactgtttgc catcaacgcg caggcggcct gcgacttcat
     8821 cccggtcggt ttgtcgctgg cggaagcccg tcaggacacg gttcgcgtcg gtgtcccttc
     8881 tgtactggtg agccactttt taaccggcgc accgactgta ctgatcgcct ggtttgtctc
     8941 cggttttatc tatcaataga ggctgaaaca tgaccgttat ttatcagacc accatcaccc
     9001 gtatcggcgc gagtgccact gacgccctca gcgaccagat gctcatcacc tttcgtgaag
     9061 gcgcgccagc ggacctcgaa gagtattgct tcattcattg ccacggcgag ttgaaaggtg
     9121 cactccatcc cggtttgcaa tttacgctcg ggcagcatcg ctatccggtg accgctgttg
     9181 gcagcgtggc ggaagacaac cttcgcgaac tgggtcatgt caccctgcgc ttcgatggtt
     9241 taaacgaagc ggaatttccg ggcactgtcc atgtggcagg ccctgtaccc gacgatatcg
     9301 cgccgggatc ggttttgaag tttgaatctg ttaaggagta aaaaatgaat caggttgccg
     9361 ttgtcatcgg tggtgggcaa accttaggcg cgttcctgtg ccacggtctg gctgccgagg
     9421 ggtatcgcgt cgcggttgtc gatattcaga gcgacaaagc cgcaaatgtg gcacaagaaa
     9481 ttaacgccga atatggtgaa ggaacagcgt atagttttgg tgctgacgcc accagcgagc
     9541 aaagcgtcct ggcgctctct cgtggggtag atgaaatctt tggtcgcgtg gatttgctgg
     9601 tctacagcgc cggaatagca aaagcagcct ttatcagcga cttccagctc ggcgattttg
     9661 accgtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttctcgttt gatgatccgc
     9721 gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt gggcagcaaa
     9781 cacaactctg gctacagcgc agcgaaattt ggtggcgtcg ggctgactca atcactggcg
     9841 ctggatctgg cggagtacgg cattacggtg cattcactga tgctcggtaa cctgctgaaa
     9901 tcgcctatgt tccagtcact gttaccccaa tacgcgacca agctgggtat caaaccggat
     9961 caagtcgagc agtattacat cgacaaagta ccgctcaaac gcggctgcga ttatcaggat
    10021 gtgctgaata tgctgctgct ctatgccagt cctaaggcgt cgtactgcac cggacagtcg
    10081 atcaatgtca ccggcggtca ggtgatgttc taatcaacag cggagatccg ttaaggatct
    10141 ccgtgagact aatagaatgc ctgatgcgct acgctcatca ggcatacagg acttccgcca
    10201 ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat cgcctggtgt
    10261 gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt cgacacacta
    10321 tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc gctttaaacc acgggtcgtg
    10381 gtcgccatcg ccctggacga tcagcagcgc atcgtcgaca ccttgtttat gaaaggactg
    10441 accgtcttcg cacgaccgca aaaaattccc gcaattaccg gtatgcatgc gggtgattta
    10501 cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc attggcgctt
    10561 aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt atcattttga
    10621 aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc cattctggag
    10681 tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt tgacaccaca
    10741 ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg caggaaccgt cattcgtact
    10801 tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca taaaacactc
    10861 atcaacaccc acaagaaaga gctgatagca gaagctgccg ttagttttat tcatgatggc
    10921 gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggttcccct gctctcgcgc
    10981 tttaataaca tcacggtgat gaccaacagt ctgcatatcg tcaatgcgct atctgaactg
    11041 gataacgaac aaactatcct gatgccaggc ggaacgtttc gtaaaaaatc ggcctcattt
    11101 cacggacagc tggcagagaa tgccttcgaa catttcagtt tcgataaatt gtttatgggc
    11161 accgacggca tcgatctcaa tgcgggcgta accaccttta acgaggttta taccgtcagt
    11221 aaggcaatgt gcaatgccgc gcgcgaagtg attttgatgg cggactcatc aaagtttggc
    11281 cgtaaaagcc ccaacgtagt ttgcagtctt gaaagcgtcg ataagctgat taccgacgca
    11341 ggtatcgatc cggcgtttcg tcaggcgctg gaagagaaag ggatcgatgt gatcataacc
    11401 ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa tgctggagtt
    11461 gcaggaagca agccgtttac cggaacgtct gggcgatgat tttgttcgcg ccgccaatat
    11521 catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg gccacattgg
    11581 taagaaaatc gccgcaacac ttgccagtac cggcacatcg gctttttttg tccatccggc
    11641 agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc tgtttatctc
    11701 ttactccggt ggcgcgaagg aactggatct gattattccg cgtctggaag ataaatctat
    11761 cgcgctgctg gcgatgaccg gcaaaccgac gtcaccgctg ggcctggcgg caaaagcggt
    11821 gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga cctccagcac
    11881 cgtcaatacc ctgatgatgg gtgacgcgct ggcgatggcg gtcatgcagg cgcgcggatt
    11941 taatgaagaa gattttgccc gctcccaccc agccggggca ctgggcgctc gcttgctgaa
    12001 taaagtgcat catctgatgc gccgtgacga tgccatccca caggtggcgt taaccgccag
    12061 cgtgatggat gcgatgctgg aactcagccg caccggtctg gggctggtgg cggtatgtga
    12121 cgctcaacaa caggtacaag gcgtctttac cgacggcgat ttacgtcgct ggctggtagg
    12181 cggcggcgca ctcaccacgc cagttaatga agcgatgacg acaggcggca ccacgttaca
    12241 ggcgcaaagt cgcgccatcg acgccaaaga gatcctgatg aagcgcaaaa tcactgccgc
    12301 accggtggtg gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg atttctatca
    12361 ggccgggatt atttaatcct tcattcccag acgtttcgcc agccgatgca ggttggcgac
    12421 gtcggtttcc agcatccgcg cgcaggcagc ccagttatga tgattttgtg ccagtgcctg
    12481 gcgaatagtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct taacaacggg
    12541 caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat gttgcgcctc
    12601 aagaatcact tcatcgccgt tgcgggtggc tctcgccagc actaccgccc gatgaatagc
    12661 atgttccagt tcgcgcacgt tccccggaaa acgatagtgt tgcagtaaat ttcgcgctcc
    12721 ggcacttaat accacgcggg agagccccag ccgcaaacga cactgctcgc agaaataccc
    12781 cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcaccgaaa gtggaaacac
    12841 gctcaggcga tgaaacaagt cagcgcgaaa tcgccctgcc atcacctctt cgcgtaagtc
    12901 gcggttagtc gccgccagca cgcgcacatc gacccgcaaa ctacggtcat cgccaacgcg
    12961 ctgaatatcg ccatactgca acaccctcag cagcttggcc tgcaatgcca acgacaactc
    13021 gccgatctca tccagaaaca gagtgccgtt atccgccatt tcaaacttcc cgctgcggtt
    13081 actgatagcg ccagtaaacg ctcctttcac atgcccgaac aactcacttt ccgccacact
    13141 ttccggcagt gcagcacagt tgagatagac cagcggattc accgcccgtg gcgaggcttc
    13201 atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctga tcaggacgtt
    13261 gagatcggac gccgccacaa tctcaatctc ttttttcagt tgcgtcatgc caggggacaa
    13321 gccaatcatc tgcgtctgtt tcaccgcttc aaacggcgtg gcatcgcctg gcatcatatt
    13381 ctggctttcc agttgttcaa tcagcaacgc attgcttaac gctcccgccg ccagcgcagc
    13441 aatcagccgt agctcttcgt cgctgaaaac atcgaactga tcgggctgca tcccgtcgag
    13501 cgtcagtgcg ccgatcaggt tttgcccggc aaacaatggc agaccaacgc aggcgtgaac
    13561 cttcagactc tcctgcccag gaatcaaacc gtcataggga tcgggcaatt cgctgtctgc
    13621 gggaaagcgc accacatccc cggcgcgggc aatcgcttcc agccgtggat gcccttccag
    13681 cgcaaagcgt ctaccgagta catcctttgc cagaccgtcg atggcaagcg gaataaactg
    13741 ccgcgaatcg taacgtagca acgcagacgc atcgcactcc agcacctgac gtagcgtggt
    13801 gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga tggcgatatt
    13861 cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac tatagatatt
    13921 gtcatatcga ccatttgatt gatagtcatt ttgactactc attaatg
//
LOCUS       DAZOZY010000104        13964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZOZY010000104 DAZOZY010000000
VERSION     DAZOZY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.104"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHURQ_002616"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHURQ_002616"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0824355.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHURQ_002617"
     CDS             2273..2662
                     /locus_tag="ACHURQ_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0824356.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHURQ_002618"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHURQ_002618"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR0824357.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHURQ_002619"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHURQ_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR0824358.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHURQ_002620"
     CDS             complement(4321..4521)
                     /locus_tag="ACHURQ_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR0824359.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHURQ_002621"
     CDS             4687..5232
                     /locus_tag="ACHURQ_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR0824360.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHURQ_002622"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHURQ_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHURQ_002623"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHURQ_002623"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR0824361.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHURQ_002624"
     CDS             complement(6529..7353)
                     /locus_tag="ACHURQ_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR0824362.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHURQ_002625"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHURQ_002625"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR0824363.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHURQ_002626"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHURQ_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR0824364.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHURQ_002627"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHURQ_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR0824365.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHURQ_002628"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHURQ_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR0824366.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHURQ_002629"
     CDS             complement(11316..11969)
                     /locus_tag="ACHURQ_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR0824367.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHURQ_002630"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHURQ_002630"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR0824368.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHURQ_002631"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHURQ_002631"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR0824369.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3390 a         3536 c         3712 g         3326 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagc
//
LOCUS       DAZOZY010000105        13904 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZOZY010000105 DAZOZY010000000
VERSION     DAZOZY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.105"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            241..1494
                     /gene="kch"
                     /locus_tag="ACHURQ_002632"
     CDS             241..1494
                     /gene="kch"
                     /locus_tag="ACHURQ_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR0824370.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1549..1722)
                     /locus_tag="ACHURQ_002633"
     CDS             complement(1549..1722)
                     /locus_tag="ACHURQ_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR0824371.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1865..3325
                     /gene="cls"
                     /locus_tag="ACHURQ_002634"
     CDS             1865..3325
                     /gene="cls"
                     /locus_tag="ACHURQ_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR0824372.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3360..3689
                     /locus_tag="ACHURQ_002635"
     CDS             3360..3689
                     /locus_tag="ACHURQ_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR0824373.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHURQ_002636"
     CDS             complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHURQ_002636"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR0824374.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTFKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHURQ_002637"
     CDS             complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHURQ_002637"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR0824375.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHURQ_002638"
     CDS             complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHURQ_002638"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR0824376.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHURQ_002639"
     CDS             complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHURQ_002639"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR0824377.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHURQ_002640"
     CDS             complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHURQ_002640"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR0824378.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHURQ_002641"
     CDS             complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHURQ_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR0824379.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11191..13866
                     /gene="adhE"
                     /locus_tag="ACHURQ_002642"
                     /gene_synonym="adhC"
     CDS             11191..13866
                     /gene="adhE"
                     /locus_tag="ACHURQ_002642"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR0824380.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3656 a         3436 c         3232 g         3580 t
ORIGIN      
        1 ttatagccgg tcagtgcatt cactaccaca agcagataat aactttatct gtatttcttt
       61 atatgatgtg agcatattca ggaaaaatga cctttcctga ggtacagttg ctcgctcaaa
      121 tcctttgatt actgcggata aagtcatcta ctattgttta acacattcac gcgctgcatg
      181 tatgttcaga aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca
      241 gtgagtcact gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac
      301 gacattctcg cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt
      361 atgtcggtca atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc
      421 ttgctggcaa acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc
      481 ttactgttcc gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta
      541 atttacaccc tgcattttta tccctggctg aaatttagta ttggattttg catttttacg
      601 ctggtgtttt tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca
      661 atttttgcat ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat
      721 ttaagcgaag gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata
      781 gaaaccatgt caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg
      841 ttcactattt cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt
      901 tttggtccgc ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg
      961 catcgtaaag atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg
     1021 caactgaatc aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc
     1081 aagcaacttg agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac
     1141 agttcagtat taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat
     1201 aacgatgcag ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa
     1261 acagttctcg ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat
     1321 atcattctct caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa
     1381 gagattaata atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc
     1441 gataacgatg aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta
     1501 aagacgaaag agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta
     1561 gatcgcaaac aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat
     1621 acgcatattt cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg
     1681 ctgcatgcgc cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa
     1741 taacagaatg gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt
     1801 atttgccaga tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta
     1861 atctatgaca accgtttata cgttggtgag ttggttggcc attctgggat actggttgct
     1921 cattgcaggc gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc
     1981 ctggctgttg attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg
     2041 cgagctccat ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc
     2101 aaaatggctt aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt
     2161 cgctgcgcca ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa
     2221 tcagctacaa ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca
     2281 gctcgcgcgc cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga
     2341 ccaggtggct gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct
     2401 cgactccgcc gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc
     2461 cggtattgaa gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat
     2521 ggacctgcgc caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag
     2581 catgaatatg gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga
     2641 tctgatggcg cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga
     2701 ctgggagatt gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc
     2761 gtttgaacag gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga
     2821 agatctcatt caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat
     2881 gacaacgccc tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca
     2941 gcgcggggtg gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg
     3001 ggccagtcgc gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga
     3061 aggcgggtta ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac
     3121 cgttaacctt gatatgcgta gtctgtggct gaatttcgag attaccctgg caatcgacga
     3181 taaaggtttt ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct
     3241 gctcgatgcc cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt
     3301 ttacttcttc agcccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta
     3361 tggatatgga tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata
     3421 tttttctcga actggctgcc gacaatctcg acccagctga tgttctgctg ttcaatcttc
     3481 agtttgaaga gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg
     3541 ttgatttcga tttgaacccg gacttttttg ccgaagtggt gattggtctg gcggacagtg
     3601 aggatggtga aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca
     3661 aactttgcca tatcatctgg cgggaataaa taatcgcttt cggcgtgaaa aagggctgac
     3721 aactgtcagc ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct
     3781 gaaactccct tccagcacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaaca
     3841 ggtacggaaa acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa
     3901 ctggatggtt ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc
     3961 cctggtgtaa ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc
     4021 atggccgaga tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg
     4081 agcgataaaa attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac
     4141 ctgcgcctga attgacacgt ccagcgccga aatcggctca tcgcagataa tcagcttcgg
     4201 ttcaagaata agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg
     4261 cggatagcgg ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg
     4321 ctcgcgaact tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat
     4381 ctcgccgatg gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat
     4441 atcactgcga acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca
     4501 ggcaacatga ccgtcggtcg ctttaaccag gccaatgatg gcgcgagcaa aggtggactt
     4561 accgcatccc gattccccta ccacacctaa tgtttcccct tcatacaggc gaagcgtgac
     4621 accatcgacg gctttgaaag ttttcggcgg ttgccagaac cactgtttgc catctttgat
     4681 ttcaaagtgc actttcagat cggcgatttc gaggaggact tttcttcctt cggttacagc
     4741 attcataaca gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct
     4801 tccagcggcg gtgcgctact acaaatttcc atcgcatgcg gacaacgtgg ctggaacggg
     4861 caacctttcg gtaatcgcag caggtttggc ggattaccag ggatggtcaa cattgtttca
     4921 ccttccgcat cgagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt
     4981 tgataaaaga catcgcgcgc gttgccatat tccatcgtgc gcccggcgta cattaccagc
     5041 actttgtcgc agatccccgc caccacgcca agatcgtggg taatcataat gatggcggta
     5101 ttaaattccc gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc
     5161 agcgcagtag tgggttcatc cgcaatcagc agcttaggtc gacatagcaa tgccatcgca
     5221 atcataactc gctgacgcat gccaccagaa aattcgtgcg ggtacatttt catgcgttta
     5281 cgtgcttccg gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct
     5341 ttgctcatgt tcttatgcag catcagcact tccatcaact gctcaccgac gcgcatatag
     5401 ggattcaacg aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta
     5461 ttgagttcac gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca
     5521 atacgtccgt tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa
     5581 ccagactcac ctacaatgcc cagcgtttct ccggcacgta gagaaaaatt caaatcattg
     5641 accgccgtga catcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc
     5701 agtgcgtcag cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct
     5761 gcactcctta acgatctttc gggtcgagag catcacgcaa gccatcgccg ataaagttga
     5821 aacaaaacag cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt
     5881 ccatcgagtt cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac
     5941 ccaaccccag gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg
     6001 cgtagaccac caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg
     6061 tcgatacacc gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt
     6121 gtccacgcac aatacgagcc atatccagcc aggaaaccat gccaatcgcc acgaaaatca
     6181 gcaggatgtt ttgaccgaaa aaggtcacaa gcaaaatgac gaagaacatg aatgggaagg
     6241 agttgaggat ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg
     6301 aaagcgaacc gtaaagtgtc cccacgacca ctgccaccag tgccgcagca acaccgacca
     6361 tgagtgagat acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag
     6421 taccaaagta gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat
     6481 cgtcataggc aaactgcgaa agcatcggtg ccaggattac aaataacgcg atcagcacca
     6541 gcactatcag actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc
     6601 acaagctgcg cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt
     6661 tcttacttaa catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc
     6721 atatagcaca tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag
     6781 gcttaacact aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc
     6841 cggcaaacca taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg
     6901 gcccatataa gagagcacgg gtaacagagc aggttttaat gcgtggcgta aaatgatccg
     6961 ccgcataggt aaccctttcg cccgggcagt acgaataaag ttggagtgta atacttcaat
     7021 catagagcct cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat
     7081 cggcaatatc atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa
     7141 aatgatcgca aatatcatga ctaataatgg cgcaaccaca aaactgggga taacaacccc
     7201 ggtcattgcc agccccatca cggtatagtc ccatttggtg ttttgtttta atgcgacaat
     7261 aacgccagca ctaacaccca gtattaccgc aaggaaaaat gcggcggctc ccagtttggc
     7321 agaaacggga aaactggatg caaccaggtc attgaccgaa taatctttat atttaaacga
     7381 tggaccgaaa tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg
     7441 atcattaaga tgatatttcg cttcgatatt ggccatcact tccggcggta aagtacgttc
     7501 gccggtaaaa gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat
     7561 aaatagcgtc ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc
     7621 ttctggcctg tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct
     7681 gccccacgta ttgccattag tgcttcacaa tgtacatatt ccgggtgtag gtattatcca
     7741 gcggatcttt gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt
     7801 aataaacagg aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag
     7861 ctgtgcgctg cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg
     7921 ggctcttata atgcgcggta ttcatcgagc tgttcgacag catggtgttc aggaaggaag
     7981 ttggttcgtt gtagtctgca caccagcctg cacgggccac atcaaaagtg ccctggtgac
     8041 gggtgtcgag gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt
     8101 tcttccacaa tgaagaggca gcaatcgcca gctttttatg cagatcagag gtgttataca
     8161 gcaggttgat ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg
     8221 cttcttcgtt acgtttttcc tggctccagc caaaccattc tggctgagtc aattttgcgc
     8281 catcagtata cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat
     8341 taacaatgat atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga
     8401 atggcggttt ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa
     8461 cttcgtccgg gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttatacg
     8521 tcatgtcgat ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca
     8581 aataggttac ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa
     8641 gaacgattcg ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat
     8701 taccaggctg ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag
     8761 ttgatgggtg aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta
     8821 atgtgtgatc atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa
     8881 tttcatcaat accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt
     8941 tcggatcaac agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgctat
     9001 cagaccattt cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc
     9061 aggattcagc gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa
     9121 acaggtctcg gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact
     9181 gaacttctga accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg
     9241 gtacatcagc tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta
     9301 ctaaacttct cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg
     9361 tattaaccag catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct
     9421 gtcaggttac tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag
     9481 acgatcacct tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc
     9541 gacgctcttt aattgttaat tattctcatt accaataaag aatcgtctgg cggttgcccg
     9601 gaaagtacca aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct
     9661 cttttattga ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa
     9721 ttcaatcaaa tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg
     9781 aaatgcacaa taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg
     9841 gaattactaa tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa
     9901 taacatgatg gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa
     9961 gaatattttt acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat
    10021 gacgaagtaa acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc
    10081 ggggaaaatc cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag
    10141 ccccataata cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg
    10201 tgccatgcgg aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc
    10261 aaacagatag ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc
    10321 tggccccgcc atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt
    10381 ttctgatttt tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat
    10441 cgctatcgtc accaccagga tgccccctgc gatacggaac gaatcaattg atataccaaa
    10501 aagttgcaga atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag
    10561 gttggctgta aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat
    10621 aaagacggga ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa
    10681 gtaaacggga aaatccaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata
    10741 atgttagcca taaattaagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt
    10801 ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac
    10861 atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa
    10921 agcgatgctg aaaggtgtca gcttagcaaa aatttgattt ggatcacgta atcagtaccc
    10981 agaagtgagt aatcttgctt acgccaccta gaagtgacgc attagagata ataactctaa
    11041 tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca
    11101 taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact
    11161 aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac
    11221 gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa
    11281 gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg
    11341 aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
    11401 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct
    11461 gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc
    11521 gttccgacca ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc
    11581 cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct
    11641 gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc
    11701 gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc
    11761 ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
    11821 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca
    11881 gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag
    11941 tctgttgttg ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc
    12001 ggctatctgt tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt
    12061 gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact ggcaggcttc
    12121 tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa
    12181 ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
    12241 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc
    12301 ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa
    12361 acggctcgta tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac
    12421 ttcaaactcg caccttccct gactctgggt tgtggttcct gggggggtaa ctccatctct
    12481 gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac
    12541 atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg
    12601 ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
    12661 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact
    12721 gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa
    12781 ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac
    12841 gccgcgaaga ttatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg
    12901 ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg
    12961 aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt
    13021 gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
    13081 atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt
    13141 ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc
    13201 tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac
    13261 cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc aactatcgcg
    13321 ggtatcgcat ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt
    13381 tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc
    13441 tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
    13501 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt
    13561 actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt
    13621 attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat
    13681 aaactgtctg aagatgcatt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg
    13741 atctccgagc tgaaacagat cctgctggat acctactacg gtcgtgatta tgtagaaggt
    13801 gaaactgcag cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc
    13861 gcttaatcag tagcgctgtc tggcaatata aacggcccct tctg
//
LOCUS       DAZOZY010000106        13853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZOZY010000106 DAZOZY010000000
VERSION     DAZOZY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.106"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHURQ_002643"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHURQ_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHURQ_002644"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHURQ_002644"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR0824381.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHURQ_002645"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHURQ_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR0824382.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHURQ_002646"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHURQ_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR0824383.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="ACHURQ_002647"
     CDS             4177..5415
                     /locus_tag="ACHURQ_002647"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR0824384.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHURQ_002648"
     CDS             complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHURQ_002648"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR0824385.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHURQ_002649"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHURQ_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR0824386.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHURQ_002650"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHURQ_002650"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR0824387.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHURQ_002651"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHURQ_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR0824388.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHURQ_002652"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHURQ_002652"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR0824389.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHURQ_002653"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHURQ_002653"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR0824390.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHURQ_002654"
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHURQ_002654"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR0824391.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHURQ_002655"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHURQ_002655"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR0824392.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHURQ_002656"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHURQ_002656"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR0824393.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHURQ_002657"
     CDS             complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHURQ_002657"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR0824394.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3400 a         3507 c         3688 g         3258 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc atccaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaacgtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tggtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca acg
//
LOCUS       DAZOZY010000107        13797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZOZY010000107 DAZOZY010000000
VERSION     DAZOZY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.107"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(894..1985)
                     /gene="ychF"
                     /locus_tag="ACHURQ_002658"
     CDS             complement(894..1985)
                     /gene="ychF"
                     /locus_tag="ACHURQ_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR0824395.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(2102..2686)
                     /gene="pth"
                     /locus_tag="ACHURQ_002659"
     CDS             complement(2102..2686)
                     /gene="pth"
                     /locus_tag="ACHURQ_002659"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR0824396.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2963..3241
                     /gene="ychH"
                     /locus_tag="ACHURQ_002660"
     CDS             2963..3241
                     /gene="ychH"
                     /locus_tag="ACHURQ_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR0824397.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="ACHURQ_002661"
     CDS             complement(3296..4975)
                     /gene="dauA"
                     /locus_tag="ACHURQ_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR0824398.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(5099..6046)
                     /gene="prs"
                     /locus_tag="ACHURQ_002662"
     CDS             complement(5099..6046)
                     /gene="prs"
                     /locus_tag="ACHURQ_002662"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR0824399.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="ACHURQ_002663"
     CDS             complement(6197..7048)
                     /gene="ispE"
                     /locus_tag="ACHURQ_002663"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR0824400.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="ACHURQ_002664"
     CDS             complement(7048..7671)
                     /gene="lolB"
                     /locus_tag="ACHURQ_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR0824401.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7885..9141
                     /gene="hemA"
                     /locus_tag="ACHURQ_002665"
     CDS             7885..9141
                     /gene="hemA"
                     /locus_tag="ACHURQ_002665"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR0824402.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            9183..10265
                     /gene="prfA"
                     /locus_tag="ACHURQ_002666"
     CDS             9183..10265
                     /gene="prfA"
                     /locus_tag="ACHURQ_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR0824403.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            10265..11098
                     /gene="prmC"
                     /locus_tag="ACHURQ_002667"
     CDS             10265..11098
                     /gene="prmC"
                     /locus_tag="ACHURQ_002667"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR0824404.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            11095..11487
                     /gene="sirB2"
                     /locus_tag="ACHURQ_002668"
     CDS             11095..11487
                     /gene="sirB2"
                     /locus_tag="ACHURQ_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR0824405.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11491..12300
                     /gene="sirB1"
                     /locus_tag="ACHURQ_002669"
     CDS             11491..12300
                     /gene="sirB1"
                     /locus_tag="ACHURQ_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR0824406.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12336..13190
                     /gene="kdsA"
                     /locus_tag="ACHURQ_002670"
     CDS             12336..13190
                     /gene="kdsA"
                     /locus_tag="ACHURQ_002670"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR0824407.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13339..13446)
                     /locus_tag="ACHURQ_002671"
     CDS             complement(13339..13446)
                     /locus_tag="ACHURQ_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0824408.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3464 a         3429 c         3522 g         3382 t
ORIGIN      
        1 attcagcgag acgatctgct ttggtattcc cattgtgttg tttgatgccc ataccactcc
       61 tatatagtac ccagactatg ggtctatgga ttttgtcatt ttgtttaaat caatgaataa
      121 tatcctctta tcatcatgat atttgtatgg tgcaatcatg atgagcttct cagagaataa
      181 ttctctatta ccgggcaaaa tcaggaacac aggaattaat atatatttaa catccccctt
      241 acaaaggtga gtttatatcg agttattccc taagttgtcg ttacttattc caattcgtta
      301 ctataaatat ggcaaaaata ttacaacaaa cagcggttaa aaaatttggt gaagattttt
      361 tgtaataatc ctaattagtt atgggaattt cgcttgatgt atctgcataa caaaaacaat
      421 atgcgccatt tttgcattac taataagaaa aagcaaaaaa catcgatatt catttttggt
      481 caatgggttt tctggcctat cgttgttatt gtgctactgg ttatcatttt catcttgcca
      541 ggatcgcacg taatcacgtt attcggggac tttatctgcg ggatattacc cttcaggtaa
      601 gtccttttct cgccagcata ttggagcgtg atcaatattg atcagctgtg aagggggtga
      661 tgtgtagttg aacaaaaact tacgttatat cactactaat gagatatccg tcatctgaca
      721 ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
      781 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
      841 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaataaat taattagacg
      901 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
      961 attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     1021 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     1081 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     1141 taagtttgca ggttcagcag cttataaccg acacggatca cacggttcag gcccggttct
     1201 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     1261 gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc gatttcacgc
     1321 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     1381 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     1441 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc taggaccgcc
     1501 agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     1561 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc aatatcgtca
     1621 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     1681 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     1741 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     1801 cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat tggtacgacg
     1861 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     1921 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     1981 cccatgattt aaatcacctt aatatcttaa taatcaatct gttattgcta acagattgca
     2041 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     2101 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     2161 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     2221 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgccagga tgaccgattc
     2281 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     2341 cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga ggcagatcca
     2401 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     2461 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acatcttcgc
     2521 ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     2581 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     2641 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt tttgtcctga
     2701 gtgtgtacat aactggcgcg tagtttactg gttgcggccc cgcttgacaa aaaactgcgt
     2761 atcaaatgca gataacgtaa taattgcctg agtggactat tagaaagtca aggtgttcag
     2821 gcgtttattt gtaaagtttt gttgaaataa gggttgtaat tgtgatcacg cccgcacata
     2881 acccacaggg tgttgtctat actttacaca taaggaagag gggtattccc tgttacaacc
     2941 cagaaagttc cggaggtgac atatgaaacg caaaaacgct tcgttactcg gtaacgtgct
     3001 catggggttg gggctggtgg taatggtggt cggcgtgggg tattcaatcc tcaaccagtt
     3061 accacagttt aatatgcccc agtatttcgc acatggtgca gtgctaagta ttttcgtcgg
     3121 tgccattctc tggctggcgg gtgcccgtgt tggcgggcat gaacaggtgt gcgaccgtta
     3181 ctggtgggtt cgtcactatg acaaacgttg ccgccgtagc gataatcgcc gtcatagcta
     3241 acaaataatg cagtttgctg accagtcagt ttgcgctgac tggtcaatct cgtacttata
     3301 aatccgccat cgccgcgcga cgattcggga agaacgcgag gcgtcccggg atcggttgaa
     3361 tgcctgcgcg agccatagtg cgcagtggct ggaattccac gttgcacacg cgcagttcac
     3421 atccttcggg cagacgcttc acaaaacgct ggaacgcatc aagaccacca gcatcaagta
     3481 ccggaacggc atcccacttc agaatcacaa tccgtttgcc ttcaagacgt gactccaggt
     3541 ccgtgaacaa gccttcagca gcagcaaaaa acagcgggcc aataacgcgc agtaccagaa
     3601 catcgtctgg aacatctacg actaccggtg ccaggcgagt catacgtgcg atacgacgca
     3661 taaacagcag cgatgccagc acgatcccca cgctgatggc aataaccata tcaaacagca
     3721 cggtcagcga catgcacagc agcatgacaa tgatgtcatc tttcggtgca tgacgcagca
     3781 agtcgaccac tttatgcgct tcactcatgt tccacgccac catcaacagc agggctgcca
     3841 tagcggaaag cggcagccag gagagcagcg gtgccagtac cagcagggca agaataacca
     3901 gaatagagtg gatcaccgcc gagatagggg aagttgcccc ggcacggacg ttagcggcag
     3961 aacgcgcgat ggcagctgta gcggtaatac caccaaagaa cggagcgata atattcccca
     4021 gtccctgtcc aaccagttcg ctgttcgcct tgtgtttcgt cccggtcata ccatccagta
     4081 ccacggcgca gagcagagat tcgattgcgc cgagcattgc cattgagaat gccgcaggca
     4141 gcagtgtgcg aatagaatcc caggttagcg tgaattctga attaggcaga tcccacggca
     4201 gcaccagttg cggcagcagt tgcggaatac cgttaccctg agaaccatcg gccaggacgt
     4261 agtggaattg cgaaccgatg gtagcaacat gtccgccgag caggttaaca atccccatca
     4321 ccgcgcaacc agccagcaag gccggaaggt gaccgggtaa acgaatgccc agacgcggcc
     4381 aaaaaacaag aatacctagc gtcacaatgc caatggcagc atcacccaca ttaatggtcg
     4441 gcagcgccat aaataatgcg ccgacttttt gtagataatg ttccgggaca tgggccattt
     4501 gcagaccgag aaaatcttta atctgcatgg taccgatggt gatcccgata cccgaggtga
     4561 aacctaaggt gacggaaacc ggaatatact caatcaggcg accaaagcgt gccagaccca
     4621 tcagaatcaa aaagatcccc gacagcaagg tcgcaaccag cagtcctgcc agtccaaact
     4681 gttgcgacac gggatagaga attaccacaa atgccgcagt cggaccggaa acgctaaagc
     4741 gtgacccacc cgtcagagca atgacaatcc ccgcaacagc tgcggtatat aaaccgtact
     4801 ggggtgccac accactacca atagccaacg ccatcgccag cgggatagca ataatcccga
     4861 cggttatccc ggcaatcagg tcacgggtaa accgtgcggc agtatatttt tctttccagc
     4921 aagcgtcgat cagagcgcgg aaaggcatca catgtgagga aaatattttg ttcacaataa
     4981 tgtttcatcc gtgagcgcat catctgtcaa ctaaatggca ggtgaaggag gcataggtca
     5041 tacaaatgga tattacagac aaaaaaaccc gccgcagcgg gtctttgagc gggttcgatt
     5101 agtgttcgaa catggcagag atcgattctt cgttgctgat acgacgaatc gcttcggcca
     5161 gcatacctga cagggtcaga gtacgcacgt tcggcagtga tttgatttca tcgctcagcg
     5221 gaatggtatc gcagacaacg acttcatcaa ttacagagtt acgcaggttg ttcgccgcgt
     5281 tgccagagaa gatcgggtga gtcgcgtacg caaatacacg tttagcacca cgttctttca
     5341 gagcttcagc agctttacac agcgtaccgc cagtgtcgat catatcatcg accagtacgc
     5401 agtcacgacc tgcaacgtca ccgatgatat gcatcacctg ggaaacgttc gcacgcggac
     5461 gacgtttgtc gatgattgcc atatcggtat cgttcagcag cttagcgata gcgcgggcac
     5521 gcacaacgcc gccgatgtcc ggagaaacca caattgggtt atccagattc agctgcagca
     5581 tgtcttccag caggatcggg ctaccaaata cgttatcaac cggaacgtcg aagaaaccct
     5641 gaatctgttc agcgtgcaga tccactgtca gcacacggtc aacaccgacg ctggagagga
     5701 agtctgcaac cactttcgca gtgattggta cacgagcgga acggacgcga cggtcctggc
     5761 gcgcatagcc aaagtagggg ataacagcgg tgatacgacc tgcggaagca cgacgcaggg
     5821 catcaaccat aacgactaat tccatcaggt tgtcgttagt aggggcacaa gtggactgga
     5881 tgatgaaaat atcaccaccg cgtacatttt cgttgatttg tacactgact tcgccgtcgc
     5941 taaagcgacc tacagcggcg tcgccgagtg aagtgtacag gcggttggca atacgttgtg
     6001 ctagttccgg ggtggcgtta ccagcaaaaa gcttcatatc aggcacgaga agaacctcag
     6061 gcatgcgtcc attggtggaa agaatctgcc gaaaactgtg cgggccaggc atgatccttt
     6121 ccaggcggtg tattaaagag cgcgatgcaa cgtctggaac aaggtgacgt tgtcaccgaa
     6181 actcagcttg cccggcttaa agcatggctc tgtgcaatgg ggaaagatta acacctttcg
     6241 ccacaaagcc attgagccat tccggggctt gctctagcac ctggcgggct tcagactctg
     6301 tatcaaattc agcaaagaca caggcccctg tcccagtcag gcgcgacggg gcgtattcta
     6361 acagccagga aagcaccgca tcaacctcgc gaaaacgttt tcttgcgata acctcgcaat
     6421 cattgctgaa ttcacatttt agcaacgttt ctattgacct ttttggcgta ttgcgcggga
     6481 gttcaggatc tttaaaaatc actggtgtcg gaatacttac accagggtgc gccaccagat
     6541 accacttctc tggcggatcc accggcgtta gtatttcacc aacgccttcg gcaaacgcgg
     6601 catgcccccg aacaaagaca ggaacatctg cgcccagcgt cagccccatt tccgccagct
     6661 catccatgct tagcccgcat tgccagagat gatttaatgc caccaggacc gtcgcggcat
     6721 tggatgaacc accgccgaga ccgccgccca tcggcaaacg cttgtcaatg ctgatattcg
     6781 caccgcttcc cgtcggaaga cgcccgctgt ctgccgcagt tttcatcaac aaccgcgctg
     6841 cgcgaacgat caggttatct tcatgttcca cgccttcaac gggcgttaac agacgaatat
     6901 ccccatcgtc acgaagctca atgctgatgg tgtcgccgta atcaagaaac tgaaacagcg
     6961 tttgcagcgt gtggtaacca tccgcacgct gaccggtaat gtataaaaac agattaagtt
     7021 ttgccggaga gggccactgt gtccgcatta tttcactatc cagttatcca tttttaactt
     7081 gatgcgttga ccaccgtcgg tgagttccat attggctggc atcgcaggtt gcgttttggt
     7141 gtcataacca ccataaacaa ccttccagtt tttgccattc tggctgtagg taatttcgct
     7201 caggcggtac tggtcgtcca gtttgtagtc ggttgcatca cccggtaaac ctaaaatcca
     7261 ttggcgcaag ctgttgagcg gaattggcat tccggtcaat ttgccaatca tctcttcggc
     7321 gtcatcggcg gtataacgct gacctttatt gtcgactaac tgcacgttac ccggttgagc
     7381 attcagctcc agttccgtgc tgcccaatgg gttagtgagc agcagacggt agcgatcctg
     7441 gccggtttgc tgccagaaaa agcgggcgta cactttttgt tggtcagaaa tataagcgaa
     7501 cgcgccgcga gtctgatact gattaagatt gcgcacgtct tgctgatgct gacgccattg
     7561 tggcgaatcc gggcttttgc caggaccttt gggcgtggta acggaacagg cagtgagcac
     7621 aagagcagcc agcggtagca ggcggataag acgaaaatcg ggcaggggca tagtgatgac
     7681 aagtccttga gatacgttgc agttataacc cttaatgcta gcgttaccgt ccgctatcgt
     7741 ctatgttcaa gttgtcttaa ttgccagaat ctaacggctt tcggcaatta ctccaaaagg
     7801 gggcgctctc ttttattgat cttacgcatc ctgtatgatg caagcagact aaccatatca
     7861 acgttggtat tatttcccgc agacatgacc cttttagcac tcggtatcaa ccataaaacg
     7921 gcacctgtat cgctgcgaga acgtgtatcg ttttcgccgg ataagctcga tcaggcgctt
     7981 gacagcctgc ttgcgcagcc gatggtgcag ggcggcgtgg tgctgtcgac gtgcaaccgc
     8041 acggaacttt atcttagcgt tgaagagcag gataacctgc aagaggcgtt aatccgctgg
     8101 ctttgcgatt atcacaatct taatgaagaa gatctgcgta aaagcctcta ctggcatcag
     8161 gataacgacg cggttagcca tttaatgcgt gttgccagcg gcctggattc actggttctt
     8221 ggggagccgc agatcctcgg tcaggttaaa aaagcgtttg ccgattcgca aaaaggtcat
     8281 atgaaggcca gcgaactgga acgcatgttc cagaaatctt tctctgtagc gaaacgcgtt
     8341 cgcactgaaa cagatatcgg tgccagtgct gtgtctgtcg cttttgcggc ttgtacgctg
     8401 gcgcggcaga tctttgaatc gctctctacg gtcacagtgt tgctggtagg cgcgggcgaa
     8461 accatcgagc tggtagcgcg tcatctgcgc gaacataaag tacagaagat gattatcgcc
     8521 aaccgcactc gcgaacgtgc ccaaattctg gcagatgaag ttggcgcgga agtgattgcc
     8581 ctgagtgaga tcgacgaacg tctgcgcgaa gccgatatca tcatcagttc caccgccagc
     8641 ccgttaccga ttatcgggaa aggcatggtg gagcgcgcat taaaaagtcg tcgtaaccaa
     8701 ccaatgctgt tggtggatat tgccgttccg cgcgatgttg agccggaagt tggcaaactg
     8761 gcgaatgctt atctttatag tgtggacgat ctgcaaagca tcatttcgca caacctggcg
     8821 cagcgtaaag ccgcagcggt tgaggcggaa actattgtcg ctcaggaaac cagcgaattt
     8881 atggcgtggc tgcgagcaca aagcgccagc gaaaccattc gcgagtatcg cagccaggca
     8941 gagcacgttc gcgatgagtt aaccgccaaa gcgttagcgg cccttgagca gggcggcgac
     9001 gcgcaagcca ttatgcagga tctggcatgg aaactgacta accgcttgat ccatgcgcca
     9061 acgaaatcac ttcaacaggc cgcccgtgac ggggataacg aacgcctgaa tattctgcgc
     9121 gacagcctcg ggctggagta gcagtacatc attttctttt tttacagggt gcatttacgc
     9181 ctatgaagcc ttctatcgtt gccaaactgg aagccctgca tgaacgccat gaagaagttc
     9241 aggcgttgct gggtgacgcg caaactatcg ccgaccagga acgttttcgc gcattatcac
     9301 gcgaatatgc gcagttaagt gatgtttcgc gctgttttac cgactggcaa caggttcagg
     9361 aagatatcga aaccgcacag atgatgctcg atgatcctga aatgcgtgag atggcgcagg
     9421 atgaactgcg cgaagctaaa gaaaaaagcg agcaactgga acagcaatta caggttctgt
     9481 tactgccaaa agatcctgat gacgaacgta acgccttcct cgaagtccga gccggaaccg
     9541 gcggcgacga agcggcgctg ttcgcgggcg atctgttccg tatgtacagc cgttatgccg
     9601 aagcccgccg ctggcgggta gaaatcatga gcgccagcga gggtgaacat ggtggttata
     9661 aagagatcat cgccaaaatt agcggtgatg gtgtgtatgg tcgtctgaaa tttgaatccg
     9721 gcggtcatcg cgtgcagcgt gttcctgcta cggaatcgca gggtcgtatt catacttctg
     9781 cttgtaccgt tgcggtaatg ccagaactgc ctgacgcaga actgccggac atcaacccag
     9841 cagatttacg cattgatact ttccgctcgt caggggcggg tggtcagcac gttaacacca
     9901 ccgattcggc aattcgtatt actcacttgc cgaccgggat tgttgttgaa tgtcaggacg
     9961 aacgttcaca acataaaaac aaagctaaag cactttctgt acttggtgct cgtatccacg
    10021 ctgcagaaat ggcaaaacgg caacaggccg aagcgtctac ccgtcgtaac ctgctgggga
    10081 gtggcgatcg cagcgaccgt aaccgtactt acaacttccc gcaggggcgc gttaccgatc
    10141 accgcatcaa cctgacgctc taccgtctgg atgaagtgat ggaaggtaag ctggatatgc
    10201 tgattgaacc gattatccag gaacatcagg ccgaccaact ggcggcgttg tccgagcagg
    10261 aataatggaa tatcaatact ggttacgtga agcaataagc caacttcagg cgagcgaaag
    10321 cccacgccgt gacgctgaaa tcttattgga gtatgtcact ggcagggggc ggacttttat
    10381 tctggccttt ggtgaaacgc agctgactga cgaacaatgt cagcaacttg atgcgctact
    10441 gacacgtcgt cgcgatggtg aacccattgc tcatttaacc ggggtgcgag aattctggtc
    10501 gttgccgtta tttgtttcgc cagcgacctt aattccgcgc ccggatacgg agtgtctggt
    10561 ggagcaggca ctggcgcggt tgcctgaaca accttgccgt attctcgatc tcgggacggg
    10621 taccggggcg attgcgcttg cgctggctag cgagcgcccg gactgcgaaa ttatcgctgt
    10681 agatcgtatg cctgatgctg tctccctggc acaacgtaat gcccagcatc tggcgatcaa
    10741 aaatatccac attctgcaaa gcgactggtt tagcgcgcta gccgggcagc agtttgcgat
    10801 gattgtcagc aatccgccgt atattgacga gcaggaccca catcttcaac aaggcgatgt
    10861 ccgctttgag ccgctcactg cgctggttgc ggcagacagt ggaatggcag acatcgtgca
    10921 tatcatcgaa cagtcgcgta acgcgctggt atccggcggc tttctgcttc tggaacatgg
    10981 ctggcagcag ggcgaagcgg tgcgacaagc atttatcctc gcggggtatc atgacgtcga
    11041 aacctgccgt gactatggtg ataacgagcg cgtaacgctc ggccgctatt atcaatgaca
    11101 agtttttcta cactgcttag tgttcatctt attagtatcg cgctttctgt tgggctatta
    11161 accttacgtt tctggctacg ttatcagaag catccacagg catttgctcg ctggacgcgc
    11221 attgtgccgc cggttgtcga tacggtgtta ttgttaagcg gcattgcgtt gatggctaaa
    11281 gcgcacatcc tgccattttc cgggcaggca cagtggctga ctgaaaagct gtttggagtt
    11341 atcatttata tcgttttggg ttttattgca ctcgattatc gtcgtatgca cagtcagcag
    11401 gcgcgcatta ttgccttccc gctggcgttg gtggtgctgt acatcatcat taaactcgcc
    11461 accacaaaag taccgttact ggggtaagtc atgagatcgt tagctgattt cgaatttaat
    11521 aaagcgccat tgtgcgaagg catgatcctg gcttgcgaag caatccgccg cgattttccc
    11581 tcgcaagatg tttacgacga actggagcgt ctcgttagtc tggcgaagga agaaatcagc
    11641 cagcttctgc ctttagaaga gcagctggaa aaactgatcg cgctgtttta cggcgactgg
    11701 ggatttaaag cctcacgcgg cgtttatcgt ctttccgatg cattatggct ggaccaggtg
    11761 ttaaagaatc gacagggcag tgcggtatca ttaggtgcgg ttttattatg ggtcgcgaat
    11821 cgtctcgatt tgccgctgct gccggtgatt ttccctacgc agctgatatt gcgcattgaa
    11881 tgtccggatg gcgaaatttg gctgattaat ccttttaacg gtgaatcgtt aagcgaacat
    11941 atgctggacg tatggttaaa gggaaatatc agcccgtcgg cggaactgtt ttatgaagac
    12001 cttgatgaag ctgataacat tgaggtaatc cgcaaattgt tggatacact caaagcctcg
    12061 ttgatggaag aaaatcagat ggagctggcg ttacgcacca gcgaagcttt attacaattc
    12121 aacccggaag atccctatga aattcgcgat cgcgggttga tttatgcgca actggattgc
    12181 gaacacgttg cgttgaacga tttaagttat ttcgttgaac agtgtccgga agacccgatc
    12241 agcgaaatga tccgtgcgca aataaataac atcgcgcata aacatattgt gctgcattaa
    12301 ttaatcgaca ttttactcaa gattaaggcg atcctatgaa acaaaaagtg gttagcattg
    12361 gcgacatcaa cgtagcaaat gacctgtcgt tcgtactgtt tggtggtatg aacgtgctgg
    12421 aatctcgcga tctggcgatg cgcatttgcg agcactacgt aaccgtgacc cagaaactgg
    12481 gtatccctta cgtgttcaaa gcctcttttg acaaagccaa ccgctcctcc atccactctt
    12541 atcgtggacc gggcctggaa gaagggatga aaatcttcca ggagttgaag cagacttttg
    12601 gcgtgaaaat tatcaccgac gttcacgaac caagtcaggc acagcccgtt gctgatgtcg
    12661 tggatgtgat tcagttgccg gcgtttcttg ctcgccagac tgacctggtt gaagccatgg
    12721 cgaaaaccgg tgcggtaatt aacgtcaaga aaccacagtt tgtcagcccg ggacagatgg
    12781 gtaatatcgt tgataaattc aaagaaggcg gcaacgaaaa agtgattctt tgcgatcgcg
    12841 gtgctaactt cggctatgac aacctggttg tcgatatgct gggcttcagc attatgaaga
    12901 aagtgtctgg taactcgccg gtgattttcg acgtgaccca cgcactgcaa tgccgcgatc
    12961 cgtttggcgc agcttccggt ggtcgtcgtg ctcaggtggc tgagctggca cgagccggta
    13021 tggcggtagg tctggcgggg ctgtttattg aagcgcatcc ggatccggaa catgcgaaat
    13081 gtgatggtcc atccgcgctg ccgctggcta aactggaacc gttcctcaag cagatgaaag
    13141 cgattgatga tctggtgaaa ggtttcgaag aactggatac cagcaagtaa tcttttttgc
    13201 ttgaaaaata aagtcttagc gttctgcgtt aagacttttt tcatgggtgc cagatacaaa
    13261 aaaggccgca ggctgttacc cctgcggccg gtttcgggcg catattgcca tcacggcagc
    13321 ctgacgcccg ttttcacctt acttccggtt acgccaccag ccgacaatcg ctgcggtaat
    13381 aattcccgcc aggatcggtg ctgccaggtc gtgccagaaa atcatggcaa actgcgcgag
    13441 cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg ctattcttga attgttctgg
    13501 ttcaagatta gcccccgttc tgttgtcagg ttaaacctct caacgtgcgg gggttttctc
    13561 tttccagcaa ccaatgccac cagggataaa gcccccgcaa cattgcgcct caccggataa
    13621 cgccggcttg atgtggatac tacgtctcaa ttcatcttca cttcatccct gaaatgtttg
    13681 caatgaagag tgcattccgg tttttcaaca gctgttacag tcgtttcatg agtgctctgg
    13741 atgatgcttc cagctcgggt tgccaatatt tacttgtgga ggagataaag acaaaaa
//
LOCUS       DAZOZY010000108        13763 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZOZY010000108 DAZOZY010000000
VERSION     DAZOZY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13763
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.108"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /gene="yojO"
                     /locus_tag="ACHURQ_002672"
     CDS             complement(<1..101)
                     /gene="yojO"
                     /locus_tag="ACHURQ_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR0824409.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYK"
     gene            210..704
                     /gene="napF"
                     /locus_tag="ACHURQ_002673"
     CDS             210..704
                     /gene="napF"
                     /locus_tag="ACHURQ_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR0824410.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            694..957
                     /gene="napD"
                     /locus_tag="ACHURQ_002674"
     CDS             694..957
                     /gene="napD"
                     /locus_tag="ACHURQ_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR0824411.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            954..3440
                     /gene="napA"
                     /locus_tag="ACHURQ_002675"
     CDS             954..3440
                     /gene="napA"
                     /locus_tag="ACHURQ_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR0824412.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3447..4142
                     /gene="napG"
                     /locus_tag="ACHURQ_002676"
     CDS             3447..4142
                     /gene="napG"
                     /locus_tag="ACHURQ_002676"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR0824413.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4129..4992
                     /gene="napH"
                     /locus_tag="ACHURQ_002677"
     CDS             4129..4992
                     /gene="napH"
                     /locus_tag="ACHURQ_002677"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR0824414.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4989..5438
                     /gene="napB"
                     /locus_tag="ACHURQ_002678"
     CDS             4989..5438
                     /gene="napB"
                     /locus_tag="ACHURQ_002678"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR0824415.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5448..6050
                     /gene="napC"
                     /locus_tag="ACHURQ_002679"
     CDS             5448..6050
                     /gene="napC"
                     /locus_tag="ACHURQ_002679"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR0824416.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6063..6686
                     /gene="ccmA"
                     /locus_tag="ACHURQ_002680"
     CDS             6063..6686
                     /gene="ccmA"
                     /locus_tag="ACHURQ_002680"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR0824417.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6683..7345
                     /gene="ccmB"
                     /locus_tag="ACHURQ_002681"
     CDS             6683..7345
                     /gene="ccmB"
                     /locus_tag="ACHURQ_002681"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR0824418.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7387..8124
                     /gene="ccmC"
                     /locus_tag="ACHURQ_002682"
     CDS             7387..8124
                     /gene="ccmC"
                     /locus_tag="ACHURQ_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR0824419.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8121..8330
                     /gene="ccmD"
                     /locus_tag="ACHURQ_002683"
     CDS             8121..8330
                     /gene="ccmD"
                     /locus_tag="ACHURQ_002683"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR0824420.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8327..8806
                     /gene="ccmE"
                     /locus_tag="ACHURQ_002684"
     CDS             8327..8806
                     /gene="ccmE"
                     /locus_tag="ACHURQ_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR0824421.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8803..10746
                     /gene="ccmF"
                     /locus_tag="ACHURQ_002685"
     CDS             8803..10746
                     /gene="ccmF"
                     /locus_tag="ACHURQ_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR0824422.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10743..11300
                     /gene="dsbE"
                     /locus_tag="ACHURQ_002686"
     CDS             10743..11300
                     /gene="dsbE"
                     /locus_tag="ACHURQ_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR0824423.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11297..12349
                     /gene="ccmH"
                     /locus_tag="ACHURQ_002687"
     CDS             11297..12349
                     /gene="ccmH"
                     /locus_tag="ACHURQ_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR0824424.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12384..13031)
                     /gene="narP"
                     /locus_tag="ACHURQ_002688"
     CDS             complement(12384..13031)
                     /gene="narP"
                     /locus_tag="ACHURQ_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR0824425.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13351..13722
                     /locus_tag="ACHURQ_002689"
                     /pseudo
     CDS             13351..13722
                     /locus_tag="ACHURQ_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2939 a         3474 c         4040 g         3310 t
ORIGIN      
        1 tgtttataac catttgaaat gtgagcaaaa gcccgttttt gccacgcttc ccgccactct
       61 tttgatcctg ctacaggttt taccccgatc ggggtatgca tctttgacac atcctttaat
      121 atcttagcgg ctataaaaat ggcttattaa ttatgcggtt tatttggtcg ctctcaattt
      181 tcagagcgcg ttaatgatgg aaggtcaatg tgaagattga tgcatcccgt cggggcatac
      241 tcactggtcg ctggcgcaaa gccagtaacg gtatccgtcc gccctggtcc ggtgatgaat
      301 ctcattttct gacccattgc acccgttgcg acgcctgtat taatgcttgt gaaaacaaca
      361 ttctgcaacg cggcgcgggc ggctatccga gcgttaattt caaaaataac gagtgcagct
      421 tctgttacgc ctgcgcgcag gcttgccccg aatcgctatt ttctccgcgc cacaccaggg
      481 cctgggattt gcagttcacc attggggacg cgtgcctggc gtatcagtca gttgaatgcc
      541 gccgctgtca ggatagctgt gagccaatgg caattatctt tcgcccgacg ctctccggga
      601 tctaccagcc gcaacttaat agccaacttt gcaacggatg tggagcatgt gcagccagct
      661 gtccggtatc agccataact gcggagtatc tccatgcaca ctaactggca agtttgcagc
      721 ctggtcgtgc aggccaaaag cgaacgaatt tcagacatca gcacccaact gaacgccttt
      781 cccggctgtg aagttgctgt cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa
      841 gcagaagaca gcgaaacgct gatccaaacc attgagtcag tacgcaacgt agagggcgtg
      901 ctggcggtgt cgctggttta tcaccagcag gaagagcaag gtgaggaaac accatgaaac
      961 tcagtcgtcg tagctttatg aaagctaacg ccgttgcggc cgctgcggcg gctgccggtc
     1021 tcagcgtgcc gggcgttgcc cgcgccgttg ttggtcagca ggaagccatt aaatgggata
     1081 aagcgccgtg ccgtttctgc ggtactggtt gcggcgttct ggtcggaacg cagcaggggc
     1141 gtgtggtggc ctgtcagggc gacccggacg caccggttaa ccgtggcctg aactgcatta
     1201 agggctattt cctgcccaag atcatgtacg gtaaagaccg tttgacgcag ccgctgctgc
     1261 gtatgaaaaa cggtaaatat gacaaagaag gcgaatttac cccaatcacc tgggatcagg
     1321 ccttcgatgt gatggaagag aaattcaaaa ccgccctgaa agaaaaaggg ccggaatcga
     1381 tcggtatgtt cggttctggt cagtggacta tctgggaagg ttatgccgcg tccaagctgt
     1441 ttaaagcggg cttccgttcg aacaacatcg acccgaacgc gcgtcactgt atggcgtcgg
     1501 cagtagttgg ctttatgcgt acctttggta tggatgagcc gatgggctgc tatgacgaca
     1561 tcgagcaggc tgacgcgttt gtgctgtggg gcgcaaacat ggcggagatg cacccgatcc
     1621 tctggtcgcg catcactaac cgtcgtctct ctaatcagaa cgtcaccgtg gcggtgcttt
     1681 ctacctacca gcatcgtagc ttcgagctgg cggataacgg catcatcttt acgccacagt
     1741 ctgacctggt gatcctgaac tacatcgcca actatatcat tcaaaacaat gcgataaatc
     1801 aggacttctt cagcaaacac gttaacctgc gcaaaggggc gacggacatc ggctacggtt
     1861 tacgtccgac ccatccgctg gaaaaagcag cgaagaatcc aggttctgac gcctccgaac
     1921 cgatgagctt tgaagattac aaagccttcg ttgccgagta tacgctggaa aaaaccgctg
     1981 aaatgaccgg cgtgccgaaa gaccagttag aacaactggc gcagctgtat gccgatccga
     2041 acaagaaagt catctcctac tggacgatgg gcttcaacca gcatactcgt ggcgtgtggg
     2101 ctaacaacct ggtctacaac ctgcacctgc tgaccggcaa aatttcccag ccgggttgcg
     2161 gtccgttctc cctgaccggg caaccttctg cgtgtggtac tgcgcgtgaa gtgggcacct
     2221 ttgctcaccg tctgcctgcg gacatggtgg tgaccaacga gaaacaccgt gatatctgcg
     2281 agaagaagtg gaatatcccg agcggcacca ttccggcgaa aatcggtctg cacgcggtgg
     2341 cacaagaccg tgcgctgaaa gacggcaaac tgaatgttta ctggaccatg tgtaccaaca
     2401 acatgcaggc cgggccgaac atcaatgaag agcgtatgcc gggctggcgc gatccgcgca
     2461 acttcatcat cgtctccgat ccgtatccga cagtcagtgc gctggccgcc gacttgatcc
     2521 tgccgaccgc aatgtgggta gagaaagagg gtgcttacgg taacgccgaa cgccgtactc
     2581 agttctggcg tcagcaggta caggcaccgg gcgaagcgaa atcggatctc tggcagttag
     2641 tccagttctc ccgccgcttc aaaactgaag aagtgtggcc ggaagagctg ctagcgaaga
     2701 aaccggaact gcgtggcaaa acgctgtacg aagtgcttta tgccacaccg gaagtgagca
     2761 aattcccggt atccgaactg gcggaagatc agctgaacga tgaatcccgc gagctgggct
     2821 tctatctgca aaaagggctg ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg
     2881 atctcgcgcc gttcgatgac taccacaaag cgcgcggtct gcgctggccg gtggtgaacg
     2941 gtaaagaaac gcagtggcgt tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag
     3001 gctataagtt ctacggtaaa ccggatggta aagcggtgat cttcgcgctg ccgttcgaac
     3061 cggcggcgga agcaccggat gaagagtacg acctgtggct ctctaccgga cgcgttctgg
     3121 agcactggca cactggcagt atgactcgcc gtgtgccgga actgcaccgc gccttcccgg
     3181 aagcggtctt gtttattcac ccgctggatg cgaaagcgcg cgatctgcgc cgtggcgaca
     3241 aagtgaaagt ggtttctcgc cgtggcgaag tgatctcgat tgttgaaacg cgcggtcgta
     3301 accgtccgcc acagggcctg gtgtacatgc cgttcttcga cgccgcacag ctggttaaca
     3361 aactaacgct ggacgcgacc gatccgctct cgaaagagac ggacttcaag aagtgcgcag
     3421 tcaaactgga gaaggtgtaa gccattatgt cccggtcagc gaaacctcaa aatggtcgcc
     3481 gccgctttct gcgcgatgtt gttcgcacag caggcgggct ggctgccgtg ggtgtggcgc
     3541 tggggttaca acagcaaacc gcacgcgcat ctggcgtgcg gttgcgcccg cccggagcca
     3601 taaacgagaa cgcctttgcc agtgcctgtg tgcgttgcgg tcagtgtgtt caggcttgcc
     3661 cttacgacac cttaaaactg gcgacgctgg cttctggcct gtcggcgggc acgccatatt
     3721 ttgtcgcacg ggatattcct tgcgaaatgt gtgaggacat tccgtgcgcc aaagtgtgcc
     3781 cgagtggtgc gctggatcgt gagatcgaat cgatcgacga cgcgcggatg gggctggcgg
     3841 tgctggtgga ccaggaaaac tgtctcaact ttcaggggct gcgctgcgat gtttgttatc
     3901 gcgaatgccc gaaaattgat gaggccatca ccctggagct ggagcgcaac acgcgtaccg
     3961 gtaagcacgc ccgctttctg ccgacggttc acagcgacgc ctgtaccggc tgcggtaagt
     4021 gcgaaaaagt gtgcgtgctg gaacaaccgg caatcaaggt gttaccgctg tcactggcga
     4081 aaggggagtt aggtcaccat taccgcttcg gctggctgga ggggaacaat ggcaaatcgt
     4141 aaacgtgacg ccgggcgcga ggcgctggtg aaaaaaggct ggtggcgcag tcaccgttgg
     4201 ctggtgttac gtcgcctttg tcagttcttc gtgctgggga tgtttttgag cggtccgtgg
     4261 tttggtgtgt ggatcttaca cggcaactac agctgtagcc tgttattcga caccgttccg
     4321 ctgaccgatc cgctgatgac gctgcaaagt cttgccagtg gtcatctgcc tgccacggtg
     4381 gcgttgaccg gtgcggtgat aatcactgtg ctctacgccc tggcaggaaa gcgattattt
     4441 tgcagctggg tctgcccgct gaacccgatt accgacttag cgaactggtt acgcaggcgc
     4501 tttgacctca atcagtctgc gacgatccct cgccacattc gctacgtgct gttggtggtc
     4561 attctgctgg gatcagcctt aaccggcacg ctcatctggg aatggatcaa ccccgtttcc
     4621 ctgatggggc gtagcctggt aatgggcttc ggcagcggcg cgctgcttat tctcgcgctg
     4681 tttttatttg atctactggt cgttgaacac ggctggtgcg ggcacatttg tccggttggc
     4741 gcgctgtatg gcgtgctggg tagcaaaggt gtgattaccg ttgcggccag cgatcggcag
     4801 aaatgtaacc gctgtatgga ttgttttcat gtttgcccgg aaccgcatgt gctacgtgcc
     4861 ccggtgctgg atgaacaaag cccggtgcag gtcaccagcc gcgattgcat gacgtgcggt
     4921 cgctgcgtgg atgtctgttc tgaggatgta tttacaataa ctacacgatg gagttcggga
     4981 gcgaaatcat gaaaagccat gacctgaaga aagcgctgtg tcaatggacg gcgatgctgg
     5041 ccctggtggt aagcggcgcg gtttgggcgg ctaatggcgt cgattttagc cagtcgccgg
     5101 aagtatccgg gacgcaggaa ggggccattc ggatgccaaa agagcaggat cggatgccgc
     5161 tgaactatgt gaatcagccg ccaatgatcc cgcatagcgt tgaaggttat caggtaacga
     5221 ccaataccaa ccgctgcttg cagtgccacg gtgtcgaaag ctatcgcacc actggcgcgc
     5281 cgcgtatcag tcctactcac tttatggaca gcgacggcaa agtgggcgcg gaagtggctc
     5341 cgcgtcgtta tttctgtctg caatgtcacg taccgcaggc cgataccgcg ccaatcgtgg
     5401 ggaatacctt taccccatca aaaggttacg ggaaataaga ggttattatg ggaaattctg
     5461 accgtaagcc tggtctgatt aagcgcctgt ggaaatggtg gcgtaccccc agccgtctgg
     5521 cgctgggaac gctgctgttg atcggttttg tcggcggcat cgtcttctgg ggcggcttta
     5581 acaccggaat ggaaaaagcc aataccgaag agttctgcat tagctgccac gaaatgcgta
     5641 acacggtgta tcaggaatac atggattccg tgcactacaa caaccgtagc ggcgtccgtg
     5701 cgacctgtcc ggattgtcac gttccgcacg agtttgtgcc gaagatgata cgcaagctca
     5761 aagcaagtaa agagctgtat ggtaaaattt ttggcgttat tgacacgccg cagaaatttg
     5821 aagctcatcg tctgacaatg gcacagaatg agtggcggcg catgaaggac aataactcgc
     5881 aggagtgccg taactgtcac aacttcgagt atatggatac aaccgcccag aaatcggttg
     5941 ccgcgaagat gcatgaccag gcggtgaaag atgggcaaac ctgtattgat tgccataaag
     6001 ggatagcgca caagctgccc gatatgcgtg aagtcgagcc aggtttttaa cagggttatt
     6061 gcgtgggtat gcttgaagcc agagagttac tttgtgagcg ggatgaacga accttattta
     6121 gtggcttgtc atttacgctg aacgcaggag agtgggtaca aatcaccggt agcaacggcg
     6181 cggggaagac aacgcttctc cgtttgctga cggggttgtc tcgccctgac gcaggcgagg
     6241 ttctctggca agggcagccc ttgcatcagg tacgcgacag ctaccatcaa aacctgttat
     6301 ggataggcca tcagccgggg atcaaaaccc ggctgacggc gttagaaaat ctgcactttt
     6361 atcatcgcga tggcgatacc gcacaatgtc tggaagccct ggcgcaggcc gggcttgccg
     6421 gattcgacga tattccggta aatcaacttt ctgccgggca acaacgccgc gtcgctttag
     6481 cgcgtctgtg gctgacccgt gccacgttat ggatcctcga cgagcctttt accgcgattg
     6541 acgttaacgg cgtcgatcgt ctgacccagc gtatggcgca gcatacggag caggggggga
     6601 ttgtgattct gactacccac cagccgctca acgttgctga aagtaaaatt cgccgcattt
     6661 cactgacgca aacgagggcc gcatgatgtt ctggcgcatt ttccgtcttg agctgcgtgt
     6721 agcgtttcgc catagcgccg aaatcgccaa cccgctgtgg ttcttcctga ttgtaattac
     6781 cctttttccg ctcagtatcg gtccggagcc gcaactgctg gcgcgtattg caccgggcat
     6841 tatctgggtt gctgcgctgc tttcatcctt gctggcgctg gaacgactgt tccgtgacga
     6901 tttgcaggac ggcagtcttg aacaattgat gttgttgccg ttacccttgc ccgccgttgt
     6961 gctggcgaag gtgatggcgc actggatggt aaccggtctg ccgttactca tcctttcgcc
     7021 actggtagca atgctactgg gaatggatgt ttatggctgg caagtgatgg cgctgacgct
     7081 gctgctggga acgcctacgc ttggctttct cggtgcaccg ggtgtggcgt tgacagtggg
     7141 acttaagcgc ggtggtgtgc tgctcagcat actggtgtta ccgctgacta tcccattact
     7201 catctttgcc accgccgcga tggacgcggc ttccatgcat ttgcccgttg acgggtatct
     7261 ggcaatttta ggcgcgttgc tggcaggcac cgcgacatta agtccttttg cgacggcggc
     7321 agcgttacga atcagcattc aataacgctg gattactgat ttttgttcga gtctggtatc
     7381 gaaactatgt ggaaaacact gcatcaactg gcgatcccac cacggctgta tcaaatctgt
     7441 ggctggttta taccgtggct ggcaattgcc agtgtggtcg tgcttaccgt cggctggatc
     7501 tggggattcg gctttgctcc ggctgattat cagcagggaa atagctaccg cattatctac
     7561 ctgcatgtgc ctgcggcgat ctggtcgatg ggcatttatg catcaatggc agtggcagcg
     7621 tttattggcc ttgtctggca gatgaaaatg gccaacctgg cggtggcggc gatggccccc
     7681 attggtgccg tgtttacctt tattgccctg gttaccggct ctgcatgggg aaaaccgatg
     7741 tggggcacct ggtgggtatg ggatgcacgt ctgacttctg aactggtgct gctgtttttg
     7801 tatgtgggtg tgattgccct gtggcacgcc ttcgacgacc gccgtctggc gggccgtgcg
     7861 gcaggtatcc tggtgctgat tggcgtggtg aatctgccga ttattcatta ctccgtggag
     7921 tggtggaaca ccctgcatca gggatcaacg cggatgcagc aaagtatcga tccggcgatg
     7981 cgttcgccgc tgcgctggtc gatttttggc ttcctgctcc tgtctgccac gctgacgctg
     8041 atgcggatgc gtaatttgat tttgctgatg gaaaaacgcc gtccgtgggt gagtgaactg
     8101 atactgaaaa gaggccgtaa atgacccctg catttgcttc ctggaatgaa tttttcgcaa
     8161 tgggcggtta cgcctttttt gtctggctgg cggtggtgat gaccgttatt ccgctggtgg
     8221 ttttggtcgt gcactcggtg atgcaacatc gcgcaattct gcgtggtgtg gcgcaacagc
     8281 gggcgcgtga ggcgcgttta cgtgctgcgc aacagcagga ggctgcatga atattcgccg
     8341 taaaaaccgc ttgtggattg cctgtgccgt gttggcaggg ctggcgctga ctatcggtct
     8401 ggtgctatat gcgctgcgct cgaatatcga tctcttttat acgccggggg aaattctcta
     8461 cggcaagcgt gaaactcagc aaatgccgga agtcggtcag cgtctgcgcg ttggcgggat
     8521 ggtgatgctg ggtagtgtgc agcgcgatcc caattcgctg aaagtgacct tcactattta
     8581 cgatgctgaa ggctcagtgg atgtctctta cgaaggcatt ttgccggatc tgttccgtga
     8641 agggcagggc gttgtggtgc agggcgagct ggaaaaaggc aatcatatcc tcgcgaaaga
     8701 agtgctggcg aaacacgacg aaaactacac gccgccagaa gttgagaaag cgatggaagc
     8761 taaccaccgt cgcccggcga gtgtttataa ggacccagca tcatgatgcc ggaaattgga
     8821 aacgggctgc tgtgtctggc gttagggatt gcgctgctgc tgtccgtgta tccgctatgg
     8881 ggcgtggcgc gcggagatgc gcgcatgatc gcgtcttccc gcttgtttgc ctggctgctg
     8941 tttatgtctg tggctggcgc atttctggtg ctggtcaatg ctttcgtggt caacgacttt
     9001 accgtcacct atgttgccag ctactccaat acccagcttc cggtgtggta tcgcgtggcg
     9061 gctacctggg gcgcgcatga aggctcgcta ctgctgtggg tgctgctgat gagcggctgg
     9121 acctttgcgg tggcgatttt tagtcagcgt attccgctgg atattgtggc ccgcgtactg
     9181 gcgataatgg ggatggtcag cgtcggcttt ttgctgttca ttctctttac ctctaaccca
     9241 ttctcacgca cgctgccgaa cttcccgatt gaagggcgcg atcttaaccc gctgttacag
     9301 gacccggggc tgatcttcca tccgcctctg ctttatatgg ggtacgtggg tttctcggtg
     9361 gcgtttgctt ttgccattgc ttctttgctg agcgggcgtc tggacagcac ttatgcgcgt
     9421 tttactcgcc cgtggacgct ggcggcgtgg attttcctga cgctcggcat tgtgctcggt
     9481 tccgcatggg cctattacga actcggctgg gggggctggt ggttctggga tccggtagaa
     9541 aacgcctcgt ttatgccgtg gctggtgggg actgcgctga tgcactcact ggcggtcact
     9601 gaacaacgcg ccagcttcaa agcgtggaca ttactgctgg caatcagtgc cttctcgttg
     9661 tgtctgttgg ggactttcct cgtgcgttcc ggcgtgctgg tatcggtaca cgcgtttgcg
     9721 tctgatccgg cgcgcggtat gtttatcctc gcctttatgg tgctggtgat tggaggttcg
     9781 ctgctgctgt ttgccgcgcg tggacacaaa gttcgctcac gcgtaaacaa tgcgctgtgg
     9841 tcgcgggaat ctctgctgtt agcgaacaac gttttgctgg tcgccgcgat gctggtggtg
     9901 ttgctgggga cgctgctgcc gctggtgcac aagcaactgg gactgggcag tatttcgatt
     9961 ggcgaaccgt tcttcaacac catgtttacc tggctgatgg tgccgtttgc gctactgctg
    10021 ggtgtcggtc ctctggtgcg ctgggggcgc gatcgcccac gtaaaatccg caatttattg
    10081 attatcgcct tcatctcaac gctggtgctg tctctgctgt tgccgtggct gtttgagagc
    10141 aaagttgtgg cgatgacggt gctcggcctg gcaatggcct gctggattgc ggtgctggca
    10201 attgcggaag ctgcgctgcg tatttcacgc ggcacgaaaa ccaccttcag ttattggggg
    10261 atggtggctg ctcacctggg gctggcagtg acaattgttg gcattgcttt tagccagaac
    10321 tatagcgttg agcgtgatgt gcgcatgaag tccggcgata gcgtcgatat tcatgaatat
    10381 cgcttcacct tccgtgatgt caaagaggtg actggcccga actggcgtgg cggtgtggcg
    10441 actatcggcg taacgcgaga gggtaaaccg gaaacagtgc tgtatgcgga aaaacgttat
    10501 tacaacactg ccgggtcgat gatgaccgaa gcggcgattg acggtggcat cacgcgtgac
    10561 ctgtacgccg cgctcggtga agagctggaa aacggcgcgt gggctgtgcg tctttactac
    10621 aaaccatttg ttcgctggat ttgggcgggc gggctgatga tggcgttggg cggactgctg
    10681 tgtctgtttg atcctcgcta tcgtaagcgc gtgagtccgc aaaaaactgc gccggaggct
    10741 gtatgaagcg caaagtattg ttaattccgt tgattatctt cctggcgatt gccgcggcgc
    10801 tgctgtggca gctggcgcgt aatgccgaag gggatgatcc gaccaatctg gaatcggcgc
    10861 tcattggcaa gccagtgccg aagtttcgcc tcgaatcact ggacaatccg gggcagtttt
    10921 atcaggcgga tgtgctgact cagggcaaac cagtgctgct taacgtctgg gcgacctggt
    10981 gtccgacctg ccgtgcggaa catcaatatc tgaatcagct ttctgcgcag ggcatccgcg
    11041 tagtcggcat gaactataaa gacgatcgcc agaaggcaat cagctggctg aaagagctgg
    11101 gcaatcctta cgcgctaagc ctgtttgatg gcgatggcat gttagggctg gatctcggtg
    11161 tctatggcgc gccagaaacg ttccttattg acggcaacgg catcattcgc tatcgccatg
    11221 cgggcgatct caatcctcgc gtctgggaag aagagatcaa gccgctgtgg gagaaataca
    11281 gtaaggaggc cgcacaatga ggtttttatt gggcatgctg atgctgatga tctccggctc
    11341 agcgctggcg accatcgacg tgttgcagtt taaagatgaa gcacaggaac aacagttccg
    11401 tcagctcact gaagaactgc gctgcccgaa atgccagaac aacagcattg ccgattccaa
    11461 ctcgatgatt gccaccgacc tgcgccagaa agtgtatgaa ctgatgcagg aaggtaaaag
    11521 taaaaaagag attgtcgatt atatggtggc gcgttacggc aacttcgtca cttacgatcc
    11581 gccgttaacg ccgctgaccg tgctgctgtg ggtgctgcca gtagtagcta ttggcattgg
    11641 cggttgggtc atatacgccc gctcgcggcg tcgggtacgc gtggtgccgg aagcgtttcc
    11701 tgaacaaagc gtgccggaag gtaagcgtgc cggatatgtc gcttatttgc cgggcattgt
    11761 ggtggcgtta attgtggctg gcgtcagcta ctaccagacc ggaaattatc aacaggtgaa
    11821 aatctggcag caggccacgg cacaggctcc ggcgttgctg gacagggcgc tggatccgaa
    11881 agctgatccg ctcaacgaag aagagatgtc gcgccttgcg ctggggatgc gtactcaact
    11941 gcaaaaaaat ccgggagata tagacggctg gattatgttg ggccgcgttg gcatggcgct
    12001 gggtaacgcc agtatcgcca ccgatgcata cgctactgcg tatcgcctcg atccgaaaaa
    12061 cagtgatgcc gcactgggtt atgctgaagc gttgacacgt tcatctgatc ccaacgacaa
    12121 ccgcctcggc ggcgaactgc tacgtcagct ggtgagaacg gaccatagca atatccgtgt
    12181 gctaagcatg tatgcgttta atgcctttga gcagcagcga tttggcgaag ccgttgccgc
    12241 gtgggagatg atgttgaaac tcttacctgc caacgatact cgccgtgcgg tgattgaacg
    12301 tagtatcgcg caggcgatgc aacatttgtc gccgcaggag agtaaataaa aagaggcccg
    12361 atggtgatgc catcgggcta tttttattgt gccccgcgtt gttgcaggaa cagaatggtg
    12421 gccgccacgc gtgagcggac attgagttta cgcagcagat ttcgaatatg aacttttact
    12481 gtctgctcgg aaatattcaa cacagaggca atctgtttat ttgacagccc ctgtgccagc
    12541 tcgtgcagaa catccagctc gcgctccgtc agcacgctga agggatcttc ttccgcgcca
    12601 aacatttcac gttcacgtaa gtactgattg acgcgttcgc taaagacttt gctgcctttc
    12661 gctccggcac gaatcgcttc cagcaacact tccgggtcgc tgtctttcag cagataaccg
    12721 tctgcgcctg cgtctatcag cgcaaagaca tcgctggagg catcggatac ggtcaggata
    12781 ataatttgcg cggtaacgcc atccctgcgc aaggcattga gggtatccag gccactcata
    12841 cctttcatat tgagatccag caagatcacg tcgatatcca gtctattcgc cagatcgatc
    12901 gcgctcgcgc cgtcgcccgc ttcggcgacc acttcaaagc caggatcaag ctccagtaac
    12961 tgacgaacac cgcgtcgcat aagtggatga tcatccacaa tcatcacctg aaaaggcgtt
    13021 gcttcaggca tagtagtctc ctgaggtttt attagaatca ttatgtttga gcgtagcgtt
    13081 ggtaacgcta ccaggcgcgt gaatgttacc ccattttatg tatggacatg tagtgaaaac
    13141 tacaaccttg ccgttcgctt tggcatcatt ttcattataa agacggaatt ttgcatcagc
    13201 ttctcatcaa gaaaaacaac taaaaaatca atcacaatac ttgtgcattt ctttaaacag
    13261 tttttaaaaa ctgactccgg gtatggagct atgggtattt tctgtaccca atgcttttaa
    13321 cagcaattaa tttcatagga tgaaagctca atgcatcaat ctggttctgt ttctctttgt
    13381 cgttccgcaa tatctgttct ggtggctaca gcgttatatt cacccatagc attggcatca
    13441 acagttgagt atggtgagac agttgatggt gttggcctgg aaaaagatat ccagctggtt
    13501 tatgggaccg ccaataatac gaaaatcaat cctggcggag aacagcatat aaaagaattt
    13561 ggtgtaagta gtaatactga aattaacggc gggtatcagt acattgaaat gaatggcact
    13621 gcagaatact cagtattaaa tgatggttat caaattgttc aaatgggtgg cgcggtaaac
    13681 cagactacgc tcaataatgg agtgctacag gtttatggcg ctagactggc cccctgaatc
    13741 tccagacaac cagtatcact tat
//
LOCUS       DAZOZY010000109        13559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZOZY010000109 DAZOZY010000000
VERSION     DAZOZY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.109"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            54..1820
                     /gene="adeD"
                     /locus_tag="ACHURQ_002690"
     CDS             54..1820
                     /gene="adeD"
                     /locus_tag="ACHURQ_002690"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR0824426.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(1865..3256)
                     /gene="uhpT"
                     /locus_tag="ACHURQ_002691"
     CDS             complement(1865..3256)
                     /gene="uhpT"
                     /locus_tag="ACHURQ_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR0824427.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3394..4713)
                     /gene="uhpC"
                     /locus_tag="ACHURQ_002692"
     CDS             complement(3394..4713)
                     /gene="uhpC"
                     /locus_tag="ACHURQ_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR0824428.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4723..6225)
                     /gene="uhpB"
                     /locus_tag="ACHURQ_002693"
     CDS             complement(4723..6225)
                     /gene="uhpB"
                     /locus_tag="ACHURQ_002693"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR0824429.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6225..6815)
                     /gene="uhpA"
                     /locus_tag="ACHURQ_002694"
     CDS             complement(6225..6815)
                     /gene="uhpA"
                     /locus_tag="ACHURQ_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR0824430.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6890..7180)
                     /gene="ilvN"
                     /locus_tag="ACHURQ_002695"
     CDS             complement(6890..7180)
                     /gene="ilvN"
                     /locus_tag="ACHURQ_002695"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0824431.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7184..8872)
                     /gene="ilvB"
                     /locus_tag="ACHURQ_002696"
     CDS             complement(7184..8872)
                     /gene="ilvB"
                     /locus_tag="ACHURQ_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR0824432.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(8978..9076)
                     /gene="ivbL"
                     /locus_tag="ACHURQ_002697"
     CDS             complement(8978..9076)
                     /gene="ivbL"
                     /locus_tag="ACHURQ_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR0824433.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9641..9730
                     /gene="tisB"
                     /locus_tag="ACHURQ_002698"
     CDS             9641..9730
                     /gene="tisB"
                     /locus_tag="ACHURQ_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR0824434.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9854..9928)
                     /gene="ysdE"
                     /locus_tag="ACHURQ_002699"
     CDS             complement(9854..9928)
                     /gene="ysdE"
                     /locus_tag="ACHURQ_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR0824435.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10010..11194
                     /gene="emrD"
                     /locus_tag="ACHURQ_002700"
     CDS             10010..11194
                     /gene="emrD"
                     /locus_tag="ACHURQ_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR0824436.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11202..11699)
                     /locus_tag="ACHURQ_002701"
     CDS             complement(11202..11699)
                     /locus_tag="ACHURQ_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR0824437.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11696..12058)
                     /locus_tag="ACHURQ_002702"
     CDS             complement(11696..12058)
                     /locus_tag="ACHURQ_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR0824438.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12048..12395)
                     /locus_tag="ACHURQ_002703"
     CDS             complement(12048..12395)
                     /locus_tag="ACHURQ_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR0824439.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12504..12953
                     /gene="yidI"
                     /locus_tag="ACHURQ_002704"
     CDS             12504..12953
                     /gene="yidI"
                     /locus_tag="ACHURQ_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR0824440.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13000..13158)
                     /locus_tag="ACHURQ_002705"
                     /pseudo
     CDS             complement(13000..13158)
                     /locus_tag="ACHURQ_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13202..>13559)
                     /locus_tag="ACHURQ_002706"
     CDS             complement(13202..>13559)
                     /locus_tag="ACHURQ_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0824441.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3207 a         3777 c         3538 g         3037 t
ORIGIN      
        1 cgtttcttta tgataaataa taatcaaatt gataaaatca aaatgagaaa aatatgaata
       61 attctattaa ccataaattt catcacatta gccgggctga ataccaggaa ttgttagccg
      121 tttcccgtgg cgacgctgtt gccgattata ttattgataa tgtctctatt ctcgacctga
      181 tcaatggcgg agaaatttcc ggcccaattg tgattaaagg acgttacatt gccggtgttg
      241 gcgcagaata cgctgatgct ccggctttgc agcggattga tgctcgcggc gcaacggcgg
      301 tgccagggtt tattgatgct cacctgcata ttgaatccag catgatgacg ccggtcactt
      361 ttgaaaccgc taccctgccg cgcggcctga cgaccgttat ttgcgaccct catgaaatcg
      421 tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg ctgtgccgaa caggcaaggc
      481 aaaaccagta cttacaggtc agctcttgcg tacccgccct ggaaggctgc gatgttaacg
      541 gtgccagttt tacccttgaa cagatgctcg cctggcggga ccatccgcag gttaccggcc
      601 ttgcagaaat gatggactac cctggcgtaa ttagcgggca gaatgcgctg ctcgataaac
      661 tggatgcatt tcgccacctg acgctggacg gtcactgccc gggtttgggt ggtaaagaac
      721 ttaacgccta tattactgcg ggtattgaaa actgccacga aagttatcag ctggaagaag
      781 gacgccggaa attacaactc ggcatgtcgt tgatgatccg cgaagggtcc gctgcccgca
      841 atctcaacgc gctggcaccg ttgatcaacg aatttaacag cccgcaatgc atgctctgta
      901 ccgatgaccg taacccgtgg gagatcgccc atgaaggaca catcgatgcc ttaattcgcc
      961 gcctgatcga acaacacaat gtgccgctgc atgtggcata tcgcgtcgcc agctggtcga
     1021 cggcgcgcca ctttggtctg aatcacctcg gcttactggc acccggcaag caggccgata
     1081 tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca ggtactggtg aaaggcgagc
     1141 cgattgatgc gcaaacctta caggcggaag agtcggcgag actggcacaa tccgctccgc
     1201 catatggcaa caccattgcc cgccagccag tttccgccag cgactttgcc ctgcaattta
     1261 cgcccggaaa acgctatcgg gtcattgacg tcatccataa cgaattgatt acgcactccc
     1321 actccagcgt ctacagcgaa aatggttttg atcgcgatga tgtgtgcttt attgccgtac
     1381 ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt gcttggcggc tttggactga
     1441 atgaaggtgc gctggctgcg acggtcagcc atgacagcca taatattgtg gtgatcggtc
     1501 gcagtgccga agagatggcg ctggcggtca atcaggtgat tcaggatggc ggcgggctgt
     1561 gcgtggtacg taacggccag gtccaaagtc atctgccgtt gcccattgcc ggcctgatga
     1621 gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc cttgaaagcc gccgcccgtg
     1681 aatgcggtcc gttacccgat gagccgttta ttcagatggc gtttctttct ctgccagtga
     1741 tccccgcgct aaaactaacc agtcaggggc tatttgatgt cgagaagttt gccttcacta
     1801 cgctggaagt cacggaataa taaaaaagcc cggcgtcatg ccgggcaaaa gtcaccagtt
     1861 acgtttatgc cactgtcaac tgctgaattt ttttctcgcg gcggattttg cgttcttcca
     1921 tcaccgccac tatcgccatc aggcagatac aaccaatcgc ggcgatatca agtgcggcga
     1981 aggtgcctgc ccagccggta aggccgaata ccggcgtccc atcggcaatc attcccagac
     2041 ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt gcctttaata ccatcggcag
     2101 cgccaatcgc ttttttaggt acaaagccaa cagcagccac accaatcaac aattgcgggc
     2161 caaagaccag gaaacccaac gcaaagagag aagccagata gatatattcg ttactggcat
     2221 gttgatacac accgagcgtg gcgataatca gcgccagcgc gatgcaggcc accaggccac
     2281 ggcgaccgtt cgctaggtca gagagccagc cccacagcag cgtaccgacc agcgcaccag
     2341 cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga gagtttcagt tcctggaacg
     2401 cgtatacggt tgaccactgg tcgataccaa tacgtaccac atagaggaaa atgttggcga
     2461 agcacagcag ccagatcact ttgtttttca gcacatactc aacaaagatc tgccacttgg
     2521 tcatatcggt agattctgtc tctttgtcct cttcgctgat ctcctcgccg aacagttctt
     2581 cagctttgcc gaggccataa gattccgggg agtcgctgcc gtaacgcagg ccgataaaac
     2641 cgacaatcag cgcgataatc gacgggaaga taaacatgcc gatgacatgg ccatcgaaca
     2701 ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc accgccaagg ttgtgagaaa
     2761 tattccagaa accgaggaat gtcccgcgtt tacgacgcgg cgtccatttg gtgatggtgg
     2821 agtaactgca cgaaccgccg gtactctgga aaaagccgct taaggcgtag aaggcaatca
     2881 tcaggaacag gctaaccgag ccgctgccca tactggcact gaagcccagc atacaaatgg
     2941 cagacaggat cagcatgaac ggcaggaatt gtttggtgtt tttgccgtcg gcgtagtagg
     3001 aaaccagcgt tttacccacg ccataagtga tggagaaacc caggccgatc atccccagct
     3061 gcgtcatgct caacccgtag gtcgagatca tgtcgttctg cgcgatgtta aagttcttgc
     3121 gaatcaggta catcgtcagg tagccgataa agaccaccag gtaggattgc atgaacggtt
     3181 tgaaccacat tttgcgccgc acttcgagcg gaaggtccag ggtcggcttg cgaacctggt
     3241 ttaagaaagc cagcatgggt tactcctgaa atgaatacct gcgtgaggca tgcattgtaa
     3301 aaatcagcag caaatcaggc ctgagatagc gtccaggcaa aacctggaaa atttcttagt
     3361 tttgccggat atgaagtgaa aaggtgagat gcatcacgct tcgcgcggtg tctgggcgtt
     3421 caaaaagggc agtaacagca gtgcggaaat cccggcggcg atagagataa ccacaaaaaa
     3481 tccgctccag tgccaggtat cgagtacttt cgccagcggc caaccagcaa gcgacgcccc
     3541 cagataagca aacaagccga caaaccccgt cgccgcccct gccgcctctt tgtgggaaca
     3601 ctctgccgcc gccataccga ttaacatctg tgggccaaag acaaaaaaac caatggtgaa
     3661 gaagcaggtt gcctgcatca cgtagctggc aaatggcatc agccacaggg agccgactga
     3721 aagcaaaatt ccggcggcga aaatcaaatt catcggccct cggttgccgt taaacaattt
     3781 gtccgagccc caacccgcta ccagcgcacc gataaatcca cccagttcaa acatcgtcac
     3841 tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg gacatataca aattgcccca
     3901 gtcgttgatc gccgcccgga ccacatagac cagcacatag caaaacgaaa gcagccagat
     3961 atacggattc agcaacacat atttggtgag gatctcttta cgcgtcagcc ctgccccttc
     4021 ttgttgttga gctatctcca gcgcgtcgtg tcgccattca ccgaccgccg gtaaacctaa
     4081 cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt cccacgacga tcgccataca
     4141 accagcaatc atcatcccgg cacgccagcc gtaatgtagt gcagccgctg ccatcacaat
     4201 gggaatgagt gcgccgccga cgttatgcgc cgtgttccat aatgcccacc aaccaccgcg
     4261 ctcggtacgt gaataccagg ccgttaacag acgcgcacac accggtgaac cccagccctg
     4321 gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat agcgacgtcg agaagccaaa
     4381 cagaatgttg ataatgcccg tggcgataag ccctatcccc ataaaataac gggcatttga
     4441 gcgatcgctg acaatgccgg agacaaactt cgacacgcca taggtaatgt aaaacagggt
     4501 cgctaacagg ccgatatcgc tacggctgag cacgccgtta gcaaggattt ctggtacggc
     4561 ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa cccagccaga tggtcagcag
     4621 aatatgccga cgccagtagc gatagcgggc atcaatttca tatttatcag ccattaatgg
     4681 cgcatcggca ggcgctttca gaaacggcaa catcatcaaa ccttagacat agcgttgagg
     4741 tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg tgtaatgtgc cacccagcgc
     4801 cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt tgcccggaac ccggcggcaa
     4861 accgctgcca tcgtcttcaa taaccagcat caaccgttca tcctgctgcc agccttgcag
     4921 ggtgaccgcg ctggcatcag catgtttcac aatgttgttc agcccttcct ggcagacacg
     4981 aaacagcgtc acgcgctggt tttcgcttaa cgctgattca tcgattcgcc attcgagatg
     5041 gctgacaata ccgcgccctt ccagctccat ttcccgcatc agtgagcgga tggcctgctc
     5101 cagggtgaga tcatccaact ggcgcggacg taaccgaccc aacaaacggc gcaccgcgtc
     5161 gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc tgcttcacgc tggcgttatc
     5221 tgccgccagc cgctgaacaa tgcccgcctg agtacgaata gcagtgatgg tctggccgat
     5281 atcatcatgc agctcacgcg ccacatcacg gcgcacgctc tcttcggttt ccagcaaccg
     5341 ttcagccaga tgctgattgc gcgccagttc cttttgcagc gactggttaa gttcacgcaa
     5401 ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt tgcaccagca gcgagagcaa
     5461 taaatccacc ggatgatcgc gccaggtttg actggcgatc agcgcgatgg cgttcatcaa
     5521 cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc agcgcgataa tcggcagcac
     5581 cagacagaat ggcgtaaagc gcgacagttc gtccggcaat cccaactgga gccagagact
     5641 gataacaaac agcagcaagt accagaccag atgtcgcccg cgccagttga ttggctgaga
     5701 aaccagtgac gggccgagcg gcagccaggt gttattggcg agatagtgcc agaacaccag
     5761 acatatcggg gccagcgtca ggccgccagt taaagtcagc aacagcgcat tccacgactc
     5821 tttgccgtgc caaagccagg gcagcgactg caacaacgcc gccgccgtta acgccgcccc
     5881 ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag cgcgagatca gcgctacggg
     5941 cagtaacgtc agtaaactac cgatcatcaa taacggaaaa tgggttaaac cgaccgcctg
     6001 cgttagccag taaatcagca gccactccgc acccagcaat acgggccagt atccgcgcgg
     6061 gcattgcagc attagcccca gacgcagacc aaacggaaat aacagcaccg ccatatcagg
     6121 gcgctcaacc agatgcaggc tgatactcca caggcaaaac catgcggcag agaagataaa
     6181 aaagcaggca ataacggtaa ttaagcggga gaacaacgtc ttcatcacca gccatcaaac
     6241 atgcggcgcg ccagctctac gtcgttactg acgcccagtt tttccatcag attggcgcga
     6301 tggacgtgta ccgttttcgg tgacaagccc agttcggcgg caatctcttt caccgccatt
     6361 ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg ttagcgggtc ctgacgaccg
     6421 gatgccagtt taatggcaat atccggcgtc agataacagc cacccgtggc aaccgtatgc
     6481 accgcagcaa tgagttcatc cgggctacag cgtttggaaa gaaagccgcg tgcccccgcg
     6541 ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg agagcataat cgtcgccata
     6601 cctttcggca gctggcttag cagctccaga ccggagatat cgggcatgga gatatcgcaa
     6661 atacacacct gcacaccgcg ccccggcagc cccgccagcg cctcgcgccc cgaaccaaac
     6721 tcggcaacta cctgcaaatc aggttccagc cccagcagct gcgcaaagcc ggagcggacg
     6781 atgaggtgat cgtctataag ggcaacggtg atcatggtct tgtcctggcg ggtaaaaaaa
     6841 aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc cttgagcggt tactgaaaaa
     6901 acaccgcgat cttgttaaac atcgtcggat cggactgatt acgctgcact ttcacgacat
     6961 cttccagctt atcgatttgg cttatcatct gctccagacg ctggtcgtca ttgaccagta
     7021 gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca aagaatgcct tcaacgttaa
     7081 aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac gcccggatgg ttgcgaacgg
     7141 tgagctccag aattacgttg tcatgagttg tgttttgcat ggcttattcc tccaccattt
     7201 cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt ttcttcggca tcaatgcgca
     7261 catggatcag cgccgggcta gggcgattga tgatttcctg caatgcagcc tgcggatcgg
     7321 cttcgttatt caaatcacag gtttcgaggc cgaatccggc ggcaatctgc ataaagttga
     7381 ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta gaacagactc tgttgctgat
     7441 gcaccagccc cagcgcttcg ttgttcatca gaatgatttt gacatccagc tgattttcac
     7501 tggcggtcgc catctcctga atattcatca tcaggctgcc gtcgccggag aaacacaaca
     7561 ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc aggcaggcca aaacccatcg
     7621 tgcccagccc accggaggtc agccactggc gtgggcgatt gagcggataa gcttgcgcgg
     7681 tccacatctg atgctgaccc acatcggtgg tgataatcgc attgtcatcg acacaggcgg
     7741 caacggcgtt gatcaggccg taatggctta atggatcgca cgctttcggg attggacacg
     7801 ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc tgcacgcggt tgcgcttcca
     7861 cctgcgggat caattgcgcc agcacgtcat caacatccgc ctgaatcgcc acatgcggct
     7921 gcttgatttt acccagctct gcgcgatcga tatcgacatg aataattttg gcattcggac
     7981 agaactgctc ggttttgcca atcgcccggt catcaaaacg cgcaccgagc acaatcaaca
     8041 aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc gtgcatcccc agcataccca
     8101 gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat taaagtcatg gtggtaggca
     8161 gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc attgatcaca ccaccaccca
     8221 gataaagcac cgggcgtttg gcagcgttaa tcattgtagc tacgtcacga atgctttctt
     8281 cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg ctgagcttca atctcaaaaa
     8341 ccgccgtttg tacatcctta ggaatgtcta tccacaccgg gccagggcgg cctgattgcg
     8401 caatgcggaa ggcatcgctc atgacctgcg ggagttcttc gatatgtctg accagatagt
     8461 tgtgtttggt gatggggata gagatgccgt aggtgtcgac ttcctggaag gcgtcggtgc
     8521 cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag cgggatggag tccagccgcg
     8581 catcggcaat ggcggtcacc aggttagtcg cacccggtcc gctacaggcc atacagaccg
     8641 ccggtttacc gtcggtgcgc gccattccct gagcgataaa gcccgcgccc tgttcatgac
     8701 gggccagaat atggcggatt tgcgtgcttt ggcttaaggc atcgtaaaca ggcaggatag
     8761 aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg ttccaggaaa tgaacgataa
     8821 attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc cgaacttgcc atgctccagt
     8881 ccttttcttc tgggccgact ttccgggagg tgcttaaacg aaaaaccccg cccggtttgc
     8941 gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta cggcgcattg ccgacgacca
     9001 ccaccacacg cacgacgacc actgcggcgg atggcgcagt tggtagtagt tttgcgttga
     9061 acatggaagt agtcattggg gaccttgttg gttttgtgtt taacaatatt tatacaagca
     9121 cagctttaca ggggagacaa tggaaaattt ttcagcaagg gaaaattgag gggttgatca
     9181 cgttttgtac tgaattgcag ataacaaaaa accccgccgg agcgaggttt cgtcagtcgc
     9241 ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac acagaaagta tacgtgttcc
     9301 gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt agtgcatagt tgagtatcga
     9361 tcacagtttg cgttttgtcc aaatattact gtttatttat acagtaaact tctataatat
     9421 cactgtacgc aatgtgttat gcgggggccg catcgttacc cggcgcacta agtcctggct
     9481 gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc acactgtgtt atgtcaacaa
     9541 gcacaaagtt tctccttgag ataccgcgtg cacaacagct ggcaacaggc agcggaaagg
     9601 tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt atgagcctgg tggatatcgc
     9661 cattcttatc ctcaaactca ttgttgcagc actgcaactg cttgatgctg ttctgaaata
     9721 cctgaagtaa ttcagattca agtcgcacca aaggggagcg ggtaaccgct ccccttttat
     9781 atttagcgtg cgggttggtg tcggatgcga tgctgacgta tcttatccgc cctaccatct
     9841 ctcccggcaa catttattgc cgcttttgtt tacatattct gccgctaaac aactccccat
     9901 tcctggcgta tatctggcta acattcatca atgtgataga ttcctctccc gcatttatgg
     9961 gaatgcgtag tgacttactc taattatttt tataaaagca tccgtgataa tgaaaaggca
    10021 tagaaacgtc aatttgttat tgatgttggt attactcgtg gccgtcggtc agatggcgca
    10081 aaccatttat attccagcta ttgccgatat ggcgcgcgat ctcaacgtcc gtgaaggggc
    10141 ggtgcagagc gtaatgggcg cttatctgct gacttacggt gtctcacagc tgttttatgg
    10201 cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc gtcggaatgt ccatttttat
    10261 gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg gtattgattg ccgccagcgc
    10321 gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg cgtactttgc cgcgagattt
    10381 atatgaacgg acacagttgc gccatgctaa cagcctgtta aacatgggga ttctcgtcag
    10441 tccgttgctc gcaccgctaa tcggcggtct gctggatacg atgtggaact ggcgcgcctg
    10501 ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc agtatggccc gctggatgcc
    10561 ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt accagttata aaacgctttt
    10621 cggtaacagc ggttttaact gttatttgct gatgctgatt ggcggtctgg ccgggattgc
    10681 cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg ttagggctga gcagtatgac
    10741 ggtcagtatt ttgtttattc tgccgattcc ggcagcgttt tttggcgcat ggtttgccgg
    10801 acgtcccaat aaacgcttct ccacgttaat gtggcagtcg gttatctgct gcctgctggc
    10861 tggcttgctg atgtggatcc ccgactggtt tggcgtgatg aatgtctgga cgctgctcgt
    10921 tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt cctctggcga ccagcggcgc
    10981 gatggagccg ttccccttcc tggcgggcac ggctggcgcg ctggtcggcg gtctgcaaaa
    11041 cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg ccgcaaaccg gtcagggcag
    11101 cctggggttg ttgatgacct taatgggatt gttgatcgtg ctgtgctggc taccgctggc
    11161 gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac gtcaccgcag catcgtcatc
    11221 agctccatgg gagaacgatg ctgctttatc agatcacgca tcacccgcat atgcggtgcg
    11281 gagtaagaat aaaacgcctg atagcccgca caaagcacgc tgtctggcgt ctcctgccga
    11341 gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt cacagcgcgg ttgcgggttt
    11401 tcgctgccac accatttttg taaggtggcg tcaaaaagct caatcgagat acgactgata
    11461 tctgcccgca cccaacactc gaacagggca attaaaaatt tcccccacag ctctgctgtc
    11521 accgaagcgg ccgtcagatc gccccggtca tcaaattcaa gatagggaat gagctgtacg
    11581 gagcggatgc cccgctgttt gaaattgtga taaaaatcgg caggttgcag agcatccttg
    11641 tactctacaa ccagtttatg cctgtcctct tcagcatcaa tgacctgact tcctgtcatg
    11701 cgactctctc aataaatacg ataagttgat gtatatgcgt tacagcaaac agtattgcga
    11761 gagataacac cgcgagggag atcaaaaatt tgtcacggac tacgtgaaat tgggaaaaat
    11821 cgctattcgt gacatccatt aaattgcgat tacgggtgta gtgccagagg atcagcgcca
    11881 cgatggcgag aatgccaatc gaaatccaga ataacatgcc cgcctggtgc cagttgtgtt
    11941 tgatagccaa cgccatcaac gcgccgtagc ccagcatggt acgaaaccac gccagtgatg
    12001 tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc tttgcggcta tccggcatac
    12061 aacaccagtc ccataacgat caccgccacg accatcaata ttaagctaat gattaataag
    12121 ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt tttcattgcg taaccagcgc
    12181 aggtagccat acattgctaa cccgccggaa aacagacaca acaacaacgc cagcagttca
    12241 cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa gcccaacccc cgccgccaga
    12301 aatcctagtg cggtacggat ccacgccaga aaagtacgct cattagccag cgagaagcgg
    12361 taatcgggcg cttctccaag gcgggaaatt ttcattgttg ctcctttgca atttcactaa
    12421 ctctctacct ttagcggttt tttttaagaa tttcgtccgc ccgtttcctg atatgctttt
    12481 gccatccgct acaaggaggt cagatgggta tcatagcgca aaataaaatc agttcattgg
    12541 gcatgctgtt cggtgccatc gctttaatga tggggattat ccacttttca ttcggccctt
    12601 tttccgcacc accgccaacg cttgaaagca tcgtggcgga caaaacggct gaaattaaaa
    12661 gaggtcttct cgccggaata aaaggcgaaa aaattaccac cgtggagaaa aaagaggacg
    12721 tagatgtcga taaaatcctc gatcaaagcg gtatcgcact ggccattgct gcgctacttt
    12781 gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg cattcgcggc gcgctggttt
    12841 ttggcggcgg aacgctggct ttccatacct tgctttttgg tatcggcatc gtgtgcagca
    12901 ttttgctgat attccttatc ttttccttcc tgaccggcgg ttcgctggtt taagcagcct
    12961 gaataatgcc cgccgggtga tcatcagcgg gcattatcgt cagaacttct gttttttctc
    13021 ttccaccttc accccttgtg tcggtaggcc ggtgtcatag tcacgtacca ccggcgaata
    13081 gccatcttgt gggcgtggac gaaatgcccc catccagcgc ggccgtgcat ctttacgcca
    13141 cggacgcagg ctccattgtt gacatcctcc acgccctgaa ggacgtggac ccctgctacg
    13201 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca
    13261 ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccattta
    13321 catcggcgtt cgctgtatat ccacatacct ggcatctgaa tttactttgt gacaggcgat
    13381 tttctttcgc tgtatgacca cagcacacgc aacgctggct tgtgtacgct ggcggaacag
    13441 caagcacctg accaccacgc cagagctgct tgtacgcaag ctggcggcgc atttcatacc
    13501 agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggc
//
LOCUS       DAZOZY010000110        13260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZOZY010000110 DAZOZY010000000
VERSION     DAZOZY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.110"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHURQ_002707"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHURQ_002707"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0824442.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHURQ_002708"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHURQ_002708"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR0824443.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHURQ_002709"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHURQ_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR0824444.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHURQ_002710"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHURQ_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR0824445.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHURQ_002711"
     CDS             complement(4036..5568)
                     /locus_tag="ACHURQ_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR0824446.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHURQ_002712"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHURQ_002712"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR0824447.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHURQ_002713"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHURQ_002713"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR0824448.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHURQ_002714"
     CDS             complement(8663..10399)
                     /locus_tag="ACHURQ_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR0824449.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHURQ_002715"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHURQ_002715"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR0824450.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHURQ_002716"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHURQ_002716"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR0824451.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHURQ_002717"
     CDS             complement(12121..12855)
                     /locus_tag="ACHURQ_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR0824452.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13260
                     /locus_tag="ACHURQ_002718"
     CDS             13056..>13260
                     /locus_tag="ACHURQ_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR0824453.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3367 a         3373 c         3487 g         3033 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aatcagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
//
LOCUS       DAZOZY010000111        13231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZOZY010000111 DAZOZY010000000
VERSION     DAZOZY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.111"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..2066
                     /locus_tag="ACHURQ_002719"
     CDS             <1..2066
                     /locus_tag="ACHURQ_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR0824454.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2082..3101
                     /locus_tag="ACHURQ_002720"
     CDS             2082..3101
                     /locus_tag="ACHURQ_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR0824455.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3183..3521)
                     /gene="rcnB"
                     /locus_tag="ACHURQ_002721"
     CDS             complement(3183..3521)
                     /gene="rcnB"
                     /locus_tag="ACHURQ_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR0824456.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3740..4564)
                     /gene="rcnA"
                     /locus_tag="ACHURQ_002722"
     CDS             complement(3740..4564)
                     /gene="rcnA"
                     /locus_tag="ACHURQ_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR0824457.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4685..4957
                     /gene="rcnR"
                     /locus_tag="ACHURQ_002723"
     CDS             4685..4957
                     /gene="rcnR"
                     /locus_tag="ACHURQ_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR0824458.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5049..5145
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5180..5968
                     /gene="thiM"
                     /locus_tag="ACHURQ_002724"
     CDS             5180..5968
                     /gene="thiM"
                     /locus_tag="ACHURQ_002724"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR0824459.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5965..6765
                     /gene="thiD"
                     /locus_tag="ACHURQ_002725"
     CDS             5965..6765
                     /gene="thiD"
                     /locus_tag="ACHURQ_002725"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR0824460.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6830..7648
                     /locus_tag="ACHURQ_002726"
     CDS             6830..7648
                     /locus_tag="ACHURQ_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFR0824461.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7700..8446
                     /locus_tag="ACHURQ_002727"
     CDS             7700..8446
                     /locus_tag="ACHURQ_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR0824462.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8420..9385)
                     /locus_tag="ACHURQ_002728"
     CDS             complement(8420..9385)
                     /locus_tag="ACHURQ_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR0824463.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9382..10386)
                     /locus_tag="ACHURQ_002729"
     CDS             complement(9382..10386)
                     /locus_tag="ACHURQ_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR0824464.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10383..11660)
                     /locus_tag="ACHURQ_002730"
     CDS             complement(10383..11660)
                     /locus_tag="ACHURQ_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR0824465.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11917..12969
                     /gene="fbaB"
                     /locus_tag="ACHURQ_002731"
     CDS             11917..12969
                     /gene="fbaB"
                     /locus_tag="ACHURQ_002731"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR0824466.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         3473 a         3183 c         3375 g         3200 t
ORIGIN      
        1 gtggctatgt tccaccggaa aactgggagc ggggtattaa tgcgttttat acctcttatt
       61 atgtgagtca gtattacagt gactataaag cgtcgggtaa tagtaagagt acatatgtac
      121 gttttaacag cgggttaaat ttactggggt ggcaactgca ttctgatgcc agtttcagta
      181 aaacaaataa caatccaggg gtgtggaaaa gcaataccct gtatctggaa cgtggatttg
      241 cccaacttct cggcacgctt cgcgtgggtg atatgtacac atcaagcgat atttttgatt
      301 ctgttcgctt cagaggtgtg cggttgtttc gtgatatgca gatgttgcct aactcgaaac
      361 aaaattttac gccacgggtg caggggattg ctcagagtaa cgcgctggta actattgaac
      421 agaatggttt tgtggtttat cagaaagagg ttcctcctgg cccgtttgcg attacagatt
      481 tgcagttggc cggtggtgga gcagatcttg atgtcagcgt gaaagaggcg gacggttcgg
      541 taaccaccta tctggtgcct tatgcagcgg tgccaaatat gctgcaaccc ggcgtgtcga
      601 aatatgattt tgcggcgggt cgtagccata ttgaaggggc gagcaaacat agtgattttg
      661 tccaggcggg ttatcagtat ggttttaata acttattgac gttgtatggt ggctcgatgg
      721 ttgcgaataa ttattacgcg ttcactttgg ggactggctg gaatacacgc attggtgcca
      781 tttccgtcga tgccacgaag tcgcatagta aacaagacaa cggcgatgtg tttgacgggc
      841 aaagttatca aattgcctac aacaaatttg tgagccaaac gtcgacgcgt tttggtctgg
      901 cggcctggcg ttattcgtcg cgtgattacc ggacatttaa cgatcacgtt tgggcaaaca
      961 ataaagataa ttatcgccgt gatgaaaacg atgtctatga cattgccgat tattaccaga
     1021 atgattttgg tcgcaaaaat agcttttccg ccaatatgag ccagtcattg ccagaaggtt
     1081 gggggtctgt gtcattaagt acgttatggc gagattactg ggggcgtagc ggcagtagta
     1141 aggattatca gttgagttat tccaacaacc tgcgacggat aagctatacc ctcgcggcaa
     1201 gccaggctta tgacgagaat catcatgaag agaaacgttt taatattttt atatcgattc
     1261 cctttgattg gggtgatgac gtttcgacgc ctcgtcggca aatatatatg tctaactcaa
     1321 cgacgtttga tgatcagggg tttgcctcaa ataatacggg attatcagga acagtaggga
     1381 gtcgggatca gttcaattat ggtgtcaacc tgagtcatca acatcaggga aatgaaacga
     1441 cagctggagc gaatttaacc tggaacgcgc cggtggcaac agtgaatggc agttatagtc
     1501 agtcgagtac ttatcgacag gctggagcca gtgtttcagg gggcattgtc gcctggtcgg
     1561 gtggcgttaa tctggcgaac cgtctttccg aaacgtttgc tgtgatgaat gcgccaggaa
     1621 ttaaagatgc ttatgtcaat gggcaaaaat atcgcacaac aaaccgtaat ggagtggtgg
     1681 tatacgacgg aatgacacct tatcgggaaa atcacctgat gctggatgtg tcgcaaagcg
     1741 atagcgaagc agaattacgt ggcaaccgga aaattgccgc accttatcgc ggcgcggttg
     1801 tactggttaa ttttgatacc gatcagcgca agccctggtt tataaaagcg ttaagagcgg
     1861 atgggcaacc attaacgttt ggttatgaag tcaatgatat ccacggtcat aatattggcg
     1921 ttgtcggcca gggaagccag ttatttattc gcaccaatga aataccgcca tcggttaatg
     1981 tagcaattga taagcaacaa ggactttcat gcacaatcac cttcggtaaa gagattgatg
     2041 aaagtagaaa ttatatttgc cagtaatagt caggtggttc tatggaaatt cgcataatgc
     2101 tatttatatt aatgatgatg gttatgcctg tgagctatgc ggcatgttat agtgagttat
     2161 ctgttcagca caatttggtt gttcaggggg attttgcact tactcaaaca caaatagcga
     2221 catatgagca taattttaat gattcgtcat gcgtgagtac aaatactatc acccctatga
     2281 gttcgtcgga tattattgtt gggctttata acgataccat aaaattaaat ttacattttg
     2341 aatggaccaa taaaaacaac atcacgttgt caaataatca gaccagtttc accagtggtt
     2401 attcagttac ggtgacacct gcggccagta atgcaaaagt gaatgtttct gcggggggcg
     2461 gcggttcagt gatgattaat ggtgttgcga cattatccag tgcttcatca tcgacacgcg
     2521 ggagtgccgc agtacaattt ctactgtgtt tattaggtgg caagtcatgg gatgcatgtg
     2581 taaatagcta cagaaatgca ttggcacaaa atgctaatct gacattgtca tacaacccga
     2641 taaccacaac ctgcaaaccg gacgatttat taattacttt agacagtatt cccgtttcac
     2701 aattaccagc cacaggtaac aaagcaacaa taaatagtaa acaaggggat attattctgc
     2761 gttgtaaaaa tttattaggt caacaaaatc aaacatcacg gaaaatgcag gtgtatttat
     2821 caagttctga tttgttagcc ggcagtaaca cgatcctgaa aggtgcagaa aataatggag
     2881 tcggatttat gcttgaaagc aatggttctc cagtcacatt tttaaatatc actgataaca
     2941 gcaaaggata cacaaattta aaagaaattg cggcaaagtc aaaacttaca gatacaacgg
     3001 tttcaattcc gataactgcc agttactacg tctacgatac aaacaaagtt aaatctggcg
     3061 cactggaggc aaccgcatta atcaacgtga aatacgacta ataacaagca agacgagcaa
     3121 gtggctaata ataaaaaata acaaggtgtc tgcatttccc cctcatgatg agggggcttt
     3181 ttttagcgat gataaaaaat ctcaccgtcg taggctttaa tgattttacc gtcggtgtcg
     3241 ctgatcaaca cgtacgcgcc acccatatag gtccagtgcg tcccggcatc aggcgcgggc
     3301 aggttacgta actgccactg cttaatgttg tactcatcgg tgcggtacat ctctgggacg
     3361 gtatcacctg gtttgaaatg cttaaagtca gcaatgaaac tacttaactc atacttagaa
     3421 attcccgagg tattggtcga acccgcggtg gctggtgcgg cccaggcggc actggtaacc
     3481 agcaaaagca cacccagcaa catcttattt ttaatggtca taaactctcc actttgggga
     3541 tctgtaatat cagtcgcata tctttcctgc catacaatat aactgacaag cgatattttg
     3601 ttagtgatcc catgatttcg taaaaagtgt aaatgaaagg atgccctaaa agcatcctta
     3661 gcgagcagag agtcagcgga aatagctgat ttaagaatca tcctgggctg aagataacat
     3721 gcaagcctaa aggattgagt tatcgcatta tgcccatgaa gccatgtacg cccatataca
     3781 caccgactaa gccaatcaac agactggaaa aatagggggc gcgtttagcg agagtgttaa
     3841 atccgctcca gcgttttgcg acctgctgaa cgctgattgc tgcgccaacg cctacggtga
     3901 caagcgttaa cgccaggcca atgctgaaac tgacgaccag tgttgcgccc agtgtcaggg
     3961 ctttcaactg aatgcatatc aacagcacgg taattgctgc cgggcagggg ataaggccac
     4021 cggttaagcc aaatagcaaa atttgccagt tggtgacctc tctaccatca aagcgtcgtt
     4081 taatgtcatt ggcatgggct cgtgcatggg catcctgata ctcgccatgt tcgtgatggt
     4141 gatgatgtcc atggtcgtgg tgatcttcgt gatcgtgatg atgatgctca taatcatact
     4201 cgtgcatatt ctccagccag ttgcgttcgc cgcgccaggt acgccagaac atccagaacg
     4261 cggtgccaat aatgatcact gcggaaatca gctggagcca cggttctgct gattgagcag
     4321 taaagcgctt gctgatcacc atcccgccaa aggcaattaa ccagaccact gcggtatgcg
     4381 aaatagttgc tgccagtccg agcatcaccg cttgtttaat ggtgcctttg atggcgatga
     4441 taaacgccgc catcatcgtt tttgagtgcc ctggttccag gccatgaagc gcaccaagta
     4501 agatggcgct ggggatgaag aaccaggcgt ttccttgctg aagaagagtt gtaaattcgg
     4561 tcatgataat aattcttagt attaattcgg caatctgatt ctactccccc cagtacctga
     4621 tactaccccc cagtagatta atagtgctat gatattttca tattcttgtt aaccaggtgt
     4681 tgccatgtct catacaatcc gtgataaaca gaaactgaaa gcgcgtgcca gtaagattca
     4741 gggccaggtc gtggcgctca agaaaatgct cgacgagccg catgaatgcg ctgcagtttt
     4801 acaacagatt gctgctatcc gtggcgcggt aaacggtctg atgcgggaag tgattaaagg
     4861 tcatctgacg gaacacatcg ttcaccaggg ggatgagcta aaacgtgaag aagatctgga
     4921 tgtcgttctg aaggtgctgg attcatatat caaataattt attaacgcga ttgtaaaact
     4981 gccgtttttc ctcgtttaca acgcgtgcgc tggacattac catcctcctc tgcgatttat
     5041 catcgcaacc aaacgactcg gggtgccctt ctgcgtgaag gctgagaaat acccgtatca
     5101 cctgatctgg ataatgccag cgtagggaag tcacggacca ccaggtcatt gcttcttcac
     5161 gttatggcag gagcaaacta tgcaagtcga cctgctgggt tcagcgcaat ctgcgcacgc
     5221 gttacacctt tttcaccaac attcccctct tgtgcactgc atgaccaatg atgtggtgca
     5281 aacctttacc gctaatacct tgctggcgct cggtgcatcg ccagcgatgg ttatcgaaac
     5341 cgaagaggcc agtcagtttg cggctatcgc cagtgccttg ttgattaacg ttggcacact
     5401 gacgcagcca cgcgctcagg cgatgcgtgc tgccgttgag caagcaaaaa gctctcaaac
     5461 accctggacg cttgatccag tagcggttgg tgcgctcgat tatcgccgcc atttttgtca
     5521 tgaactttta tcttttaaac cggcagcgat acgtggtaat gcttcggaaa tcatggcatt
     5581 agctggcatt gctaatggtg gacggggagt ggataccact gacgccgcag ctaacgcgat
     5641 acccgctgca caaacactgg cacgggaaac tggcgcaatc gtcgtggtca ctggcgaggt
     5701 ggattatgtt accgatggcc atcgcgccgt tggcattcac ggtggcgatc cgttaatgac
     5761 caaagtggta ggaactggct gtgcattatc ggcggttgtc gctgcctgct gtgcgttacc
     5821 aggcgatacg ctggaaaatg tcgcatctgc ctgtcactgg atgaaacaag ccggagagcg
     5881 cgcagtcgcc agaagcgagg ggctaggcag ttttgttcca catttccttg atgcgctctg
     5941 gcaattgacg caggaggtgc aggcatgaaa cgaattaacg ctctgacgat tgccggtact
     6001 gatccgagtg gtggtgcggg gattcaggcc gatcttaaaa ccttctcggc acttggcgct
     6061 tatggttgct cagttattac tgcactggtg gcgcaaaata cccgtggcgt acagtcggtg
     6121 tatcgcattg agcctgattt tgtcgccgcc cagctcgatt cggtgttcag cgatgtgcga
     6181 atcgatacca ctaaaatcgg tatgttggcg gaaaccgata ttgttgaagc ggtggcagaa
     6241 cggttgcaac gttatcagat ccaaaacgtg gtactcgaca ccgttatgct ggcaaaaagc
     6301 ggcgacccac tgctttcacc ttcggcagtt gccacgctac gcagtcgatt attgccacag
     6361 gtttcattaa taacgccaaa cttgcccgaa gcagccgcct tgctcgacgc gccacacgcg
     6421 cgcaccgaac aggaaatgct ggaacaaggg cgatcgctgt tggcgatggg ctgtggcgca
     6481 gtgctaatga aaggtggtca tctggatgat gagcaaagcc cggactggct gtttacccgc
     6541 gagggtgaac aacggtttac cgcaccgcgc attatgacca aaaacaccca cggtactggt
     6601 tgtacactct ctgcggcgtt ggctgcacta cgcccgcgcc atacaaactg ggctgacacc
     6661 gtacaggagg caaaaagctg gctttcatcg gcgttagccc aggccgacac gctggaagtt
     6721 ggtcacggta ttggtccggt tcaccacttc cacgcctggt ggtgagcacc gtactatagc
     6781 tgctttggtt attgtcagcc tttggcactg acattcaggg atgaaaccaa tgcagctaag
     6841 aattaccagt cgaaaaaaac tcaccgctct attatgcgct cttgggctaa tttccatcgt
     6901 tgcaatttat ccgcgtcaga cggtgaattt tttctattcg acagcagttc agattacaga
     6961 ctacatccac ttctacggtt atcgcccggt taaatctttc gctattcgta ttcctgccag
     7021 ttacaccatt cacggaatag atgtttcgcg ctggcaggag cggatcgact ggcagcgtgt
     7081 ggcaaaaatg cgcgataacg gtattcgctt acaatttgcc tttattaagg cgacggaagg
     7141 cgaaaagctg gtggacccct atttttcgcg taactggcaa ctaagccgcg aaaatggctt
     7201 gctgcgcggg gcatatcatt atttctcccc gtcggtagca gcaccggttc aggtgcgatt
     7261 atttctgcaa acggtagatt tctcacaagg cgatttccct gcggtactgg acgtagaaga
     7321 gcgaggaaaa ttatcggcaa aagaattacg caagagggta agtcagtggc taaaaatggt
     7381 cgaaaaaagt acggggaaaa agccgattat ttactcagga gccgtttttt atcacacgaa
     7441 tctggcgggc tatttcaatg aatatccgtg gtgggtggct cactactatc aacgtcgtcc
     7501 ggacaatgac ggcatggcct ggcgcttctg gcagcattcc gaccgtggac aggtagatgg
     7561 catcaatggt ccggtggatt ttaatgtgtt taatggcacg gtggaggagt tgcagggatt
     7621 cgttgatggg attaaagaaa cgccttaaat aagaagcagt gatatactcc caccagactc
     7681 cctggcagga aaaagacaaa tggaacaagc gcatacccag ctgattgccc aactgaacga
     7741 acgtatttta gcggcggata acacgccgct ttatattaag tttgccgaaa cggtaaaaaa
     7801 tgccgtgcgc agcggggtgc tggagcatgg caatattttg cccggtgagc gtgatttaag
     7861 tcagttaacc ggcgtttcgc gcattacggt gcgcaaggcg atgcaggcgc tggaagaaga
     7921 gggcgtggtg acgcgttcgc gcggttacgg cacgcagatc aacaacatct tcgaatactc
     7981 gttgaaagaa gcgcgaggtt tttctcagca ggtggtgttg cgcgggaaaa agcccgatac
     8041 gctatgggtt aacaagcgtg tcgtgaaatg tcccgaagaa gtcgcgcagc agctggcggt
     8101 cgaagcagga agtgatgtct ttttgcttaa gcgtattcgc tatgtcgatg aagaagcggt
     8161 atcgattgag gaatcgtggg ttccggcgca tttaattcat gatgttgatg ccatcgggat
     8221 ttcactttat gactatttcc gcagccagca catttaccca caacgtacgc gttcccgcgt
     8281 tagcgcccgg atgccggatg ccgagtttca gtcacatatt cagttagata gcaaaatacc
     8341 ggtgctggtg atcaagcaag tggcgcttga ccaacagcaa cggcctattg agtacagcat
     8401 tagccactgt cgcagcgatc tatacgtttt tgtgtgcgag gagtagttcc tcgcgcgttg
     8461 gcgcacaatc accgccccga tgcccgacaa cccacgacgc cactgcattg cccagtaata
     8521 cggcatccgc cagtggcaga ccagaggcca gcccggcaag tacgccacct gcatgactgt
     8581 cgcccgcccc aatggtgtct acaacttgcg ttggaaatgc cggaatgcag ccagaagcgt
     8641 cgttgctgaa ataccatgcg ccttctttat cgagacgaac gatcaacggc gcggcaaatt
     8701 tctcctgcca ttgctcgcca agtgttgtta tctctgcgga taaagcaaaa cgttcggcgg
     8761 caatctcagc ctcttgacga ttgagcgaca ctaaaggtcg acaggccatg atccgcgcca
     8821 gtaatgcatc cgggatatcg ccaatacgtg ggccaaaatc gataaacggc gtcacgtctt
     8881 gcagctcttc cagccattcc actaacaatt cgccgcaggg cgaggccagt tgataaccgg
     8941 aaaaatagag caggctgcca ggcgcaacgg ttaatcgcgc cagccactgg cgattccact
     9001 gattttccac accgctgaat gacataaaag tgcgttcgcc atccggctca accagcgcca
     9061 gacaccagcc gttatcacct tcggcgttat cgatcaggct gattaagccc tcttttgcca
     9121 tccggttgcg aataatctcc gcccacacgc cctgaccgag cggcaaggca ttacccgctt
     9181 cgatgccgag gcgctttaac gccacggcaa tattcagtgc gcagccgcca acgttaacgc
     9241 tctgctgttt cagttcgata tcgcacccac gccaggggag ggcataagcg tcggcgatca
     9301 cgtcaatgac cgccgcgccg acgaccatca ccggctgacg cgtggttaat tcaggcagca
     9361 gcgtgtgtaa tcgagcgccg ctcataccgc ctcccgttgt tgacgatatt tcgccagcgc
     9421 tgtggcatag cggttgaaat caagctgatt taccgcatcc agttccgcct ttaacgcagg
     9481 atcgatagcg ttaacgccat gcaacgcgcc gcaaattgcc gtcgccatag caccgatggt
     9541 gtctgtgtcg ccgccaaggt tagcgcacag gacggcgcag cgattcgggt cggtttgtgc
     9601 tagttcaacc agcgcaatgg cgcacggaac ggactcaata gtgctggtac ctgcgccaac
     9661 gacctggtaa agctgttcgc tggcggattc ggtgccgtcg gcattgcgca caattttcag
     9721 cgcaatctcc agccgcgccg ccagtgaggc gctgaacgta gtgatgcgtt tttgctgtgc
     9781 atgtcgcgca attgaaggca gggaatcaac aatcgctgac cagctttctc cgtcaatggc
     9841 acgagaaatc gcccatgcga tgactaccgc gcctgcaacc gccagatcgg atttatgggt
     9901 cgggctggac gccagcgcta catcatcaat aaaggaatca acatcacgcg ccggaagcaa
     9961 acaacctaat ggcgagacgc gcatcgctgc gccgttggtc acgccgttgt tttccagttc
    10021 agcaacgggt ttaccgtcgc gaatggcgtt aagcgcaatc ttcgaggtcg gacctagtac
    10081 gtttttgtta aaggcgtcga aacgcagcgc ccagtcgaga atattacgcc caatcagatc
    10141 cggatcgatc ttgccttcac gttccagtaa cgcatccgcc agacacagcg ccatcgaggt
    10201 atcgtcggtg aattcggcgc ggttaaaata acaggccgcg ttattctcct ttggtccagg
    10261 aagaaaacgg tcaatccagc caaagtgtgc tttaacgcgg ctgcgtggcc aaagctcgga
    10321 gggcatcccc atcgcatccc ctaacgcctg cccataaaga gcaccgagaa tacgttctgt
    10381 tttcatttaa cttccccttg tgtcaacgca atatcgcgat catcgacctt gatagccgta
    10441 atttcgttgt cggattcgcg gaaaaaaatc atgaacagca cggcgataat ggcaatcatc
    10501 accgcgccga aagtccacat cccggaccag ttgaaagtca gtccgtttac cggttcctga
    10561 taagcgaaca tcttttccat catcacaccg ccaagacgat agccgagcaa actgccgaag
    10621 ccctggcagc agagcgtgat cagcccctgc gcagcggtac gcatatgcac gggggctttt
    10681 ttatcgacat agatgtaagc ggtaacgtag taaaaatcgt aacttacgcc gtgaagcaaa
    10741 ataccgagga acagtaacgc gtaggtgaaa tattcatccg tactaccgta aataaagaag
    10801 ccatagcgga tcgcagcggt gaccagacca agcaataata cctttttgat accaaagcgt
    10861 ttagtgaaaa acggcaatgc cagcataaag aagatttcag agaactggcc gagcgtcatc
    10921 cagccggtgg cgtttttcat gccaacttcg gtcagataac cgttggcaaa gatgtaatag
    10981 aacgccagtg gcatcgcaaa caggaatgaa cagaagaaaa agacgaggaa gtttttatcg
    11041 cgcagcagga tcagcgcatc caggccgagc atgactttaa tatccatttt gccggtgctt
    11101 tttggtggcg tgtcgggcag gaaaaacgca aacacaccga gcagagcaga acttccggcg
    11161 gtaatcagca gcgggatgtt agtcggtgag atatcggcat accccagtat ttgcggcaag
    11221 aaaccacatg ccagaccgga ggcaatccag ccgatagtgc ccatcacacg aatgcgcggg
    11281 aaatcacgct ccacatccgg cacgttggca aaagcgatgc tgttagtcag cgcaatggtc
    11341 ggcatatagg ttagagagta agccagcagt aacggaaaga acccggcaaa agtggtctgt
    11401 tgcgcagcga aatacatcag cagcgcgcct gcgaacatca ataccgccag cactttttgc
    11461 gccgagaaaa agcggtcagt gatggagcca accagaattg gcgacaggat cgccgcaatg
    11521 gcggtacagg catacgacca gccaatttct ccggcactaa aaccgctttt acttaaccac
    11581 aaccacaatg gcacaaacca cgcgccccag ataaaccatt caacaaacat catgaacgac
    11641 agctttgctg ttgttttcat cttttaattc cttcatgaca ggtaatgtaa ggtacgcgat
    11701 gacaatacca cttaacaata ccttttaaat acctttgcgg tgaaaacttg accgcgctaa
    11761 cattttttct gatgaatcga gccaacagaa aacgctgaaa aaacatccaa aagatggaaa
    11821 aactcgcccg taacgaccag gattatgatt gcccgcaaaa ggcgggccag gacaatccat
    11881 agccgatatc caatcggaat ttacgggagc atagtaatga cagatattgc gcagttgctt
    11941 ggcaaagacg ccgacaacct tttacagcac cgttgtatga caattccttc tgaccagctt
    12001 tatctccccg gacatgacta cgtagaccgc gtaatgattg acaataatcg cccgccagcg
    12061 gtgttacgta atatgcagac gttgtacaac accgggcgtc tggctggcac aggatatctt
    12121 tctattctgc cggttgacca gggcgttgag cactctgccg gagcttcatt tgctgctaac
    12181 ccgctctact ttgacccgaa aaacattgtt gaactggcga tcgaagcggg ctgtaactgt
    12241 gtggcgtcaa cttacggcgt gttggcgtcg gtatcgcggc gctatgcgca tcgcattcca
    12301 tttctcgtca aacttaatca caacgagacg ctaagttacc cgaataccta cgatcaaacg
    12361 ctgtatgcca gcgtggagca ggccttcaac atgggcgcgg tggcggttgg tacgactatc
    12421 tattttggct cggaagagtc acgtcgccag attgaagaaa tttctgcggc ctttgaacgc
    12481 gcgcacgagc tgggcatggt gaccgtgctg tgggcctatt tgcgaaactc cgcctttaag
    12541 aaagatggcg ttgattacca tgtttccgcc gacctgaccg gtcaggcaaa ccatctggcg
    12601 gcaaccatcg gtgcagatat cgtcaaacaa aaaatggcgg aaaataacgg cggctataaa
    12661 gcaattaatt acggttacac cgacgatcgt gtttatagca aattgaccag cgaaaacccg
    12721 attgatctgg tgcgttatca gttagctaac tgctatatgg gccgggctgg gttgataaac
    12781 tccggcggtg ctgcgggcgg tgaaactgac ctcagtgatg cagtgcgtac tgcggttatc
    12841 aacaaacgcg ctggcggaat ggggctgatt cttggacgta aagcgttcaa gaaatcgatg
    12901 gctgacggcg tgaaactgat taacgccgtg caggacgttt atctcgatag caaaattact
    12961 attgcctgat acgcctcata cgaacagcac caccccgtag gacggataag gcgtttacac
    13021 tgcatccggc aacctatgcc tgatgcgacg cggaagcgtc ttatcatgcc tacatagcac
    13081 tgccacgtat gtcggaaaag gcgtttacac cgcatccggc ataaaaacac gcgcactttg
    13141 ctacggcttc cctatcggga ggccgttttt ttgcctttca ctcctcgaac agttttcata
    13201 ttgtcgtttt gtgatcgtta tctcgacatt t
//
LOCUS       DAZOZY010000112        13045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZOZY010000112 DAZOZY010000000
VERSION     DAZOZY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.112"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            73..372
                     /locus_tag="ACHURQ_002732"
     CDS             73..372
                     /locus_tag="ACHURQ_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824467.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(419..1306)
                     /gene="rarD"
                     /locus_tag="ACHURQ_002733"
     CDS             complement(419..1306)
                     /gene="rarD"
                     /locus_tag="ACHURQ_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR0824468.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1358..1825)
                     /gene="yigI"
                     /locus_tag="ACHURQ_002734"
     CDS             complement(1358..1825)
                     /gene="yigI"
                     /locus_tag="ACHURQ_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR0824469.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1990..2859
                     /gene="pldA"
                     /locus_tag="ACHURQ_002735"
     CDS             1990..2859
                     /gene="pldA"
                     /locus_tag="ACHURQ_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR0824470.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2992..4821
                     /gene="recQ"
                     /locus_tag="ACHURQ_002736"
     CDS             2992..4821
                     /gene="recQ"
                     /locus_tag="ACHURQ_002736"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR0824471.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4885..5502
                     /gene="rhtC"
                     /locus_tag="ACHURQ_002737"
     CDS             4885..5502
                     /gene="rhtC"
                     /locus_tag="ACHURQ_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR0824472.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5564..6184)
                     /gene="rhtB"
                     /locus_tag="ACHURQ_002738"
     CDS             complement(5564..6184)
                     /gene="rhtB"
                     /locus_tag="ACHURQ_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR0824473.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6295..7317
                     /gene="pldB"
                     /locus_tag="ACHURQ_002739"
     CDS             6295..7317
                     /gene="pldB"
                     /locus_tag="ACHURQ_002739"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR0824474.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7325..8125
                     /gene="yigL"
                     /locus_tag="ACHURQ_002740"
     CDS             7325..8125
                     /gene="yigL"
                     /locus_tag="ACHURQ_002740"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR0824475.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8201..9100
                     /gene="bioP"
                     /locus_tag="ACHURQ_002741"
     CDS             8201..9100
                     /gene="bioP"
                     /locus_tag="ACHURQ_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR0824476.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8988..9941)
                     /gene="metR"
                     /locus_tag="ACHURQ_002742"
     CDS             complement(8988..9941)
                     /gene="metR"
                     /locus_tag="ACHURQ_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR0824477.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10177..12438
                     /gene="metE"
                     /locus_tag="ACHURQ_002743"
     CDS             10177..12438
                     /gene="metE"
                     /locus_tag="ACHURQ_002743"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR0824478.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12560..12682)
                     /locus_tag="ACHURQ_002744"
     CDS             complement(12560..12682)
                     /locus_tag="ACHURQ_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824479.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12723..12968)
                     /locus_tag="ACHURQ_002745"
     CDS             complement(12723..12968)
                     /locus_tag="ACHURQ_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR0824480.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3085 a         3251 c         3477 g         3232 t
ORIGIN      
        1 gtatatttta attaaatata attgaaatgt atttttattc tataaacaat atcttgggtg
       61 aatggattct atatgggaaa tggtaacttt tggcagttgt tcttgggtaa agaaccttac
      121 agcaaaaaat ctgattttgt taaggcatgg ttattgtggt gggctattac catccttgtt
      181 ctatttattt taagcctgac tacgacatta aatttaagaa gtataggtat agggttaagt
      241 tttgggtttt gtcaaatcct tggtagatat acgatgtctc taggatggag taagtactgg
      301 ggagttttgg gggtatttcc tgtaacaaat attgttcttt ttcttatctt aatttttttc
      361 aaaaaaagat aaataagtca gtgggtaggc tctttaaacg gcctacccat gttaattctt
      421 aactttttct acgctgcgta taaatcgcat ccatcacaaa aattgccagc gccacccaaa
      481 taaaggcgaa agtcaccatc ttatcggcac ccggtttttc accataaaac gtcacagcca
      541 gcaggaacat cagcgtcggg ccaatgtact ggaaaaagcc taacgttgag agacgcaagc
      601 gcgtggcagc ggcggtaaaa cacaacagcg gtacggtagt gacaataccg gcggcgatca
      661 gcagtaaatt cagcgacatc gggttttgcc ccatatggct ggttgagctg tcggcaatag
      721 caaacaggta aattgccgcc acgggcagca gccacatggt ttcgattaac atgccggttt
      781 gcgcttcaac ggcaatcttc ttgcgtacca gaccgtagaa ggcaaaacta aatgccagtc
      841 ccagcgcgat aataggtagc gaaccaaaag tccacagctg gactaacacg ccacatatcg
      901 ccagaatcac cgccagccat tgcatccggc ggaatcgctc gccgaggaaa atcatcccca
      961 gcacaatgtt caccagcggg ttaataaagt aaccaaggct cgcttccagc atatggtgat
     1021 tgttcaccgc ccagataaac agtagccagt tgccaccaat cagcacggca gagactgcca
     1081 gcataaaaat tttctgtggc gtctgaatca gcgtttttaa ataggaccac tggcggcaaa
     1141 tgctcatcag caccaccata aagaaaaacg accagatcac gcgatgcgtc aggatttcat
     1201 cggcgggcac gtagtaaatc aacttgaagt acgctggcgc tataccccaa ataaaataag
     1261 cggcaagagc gagtaatacg ccctgccgcg tttgttttgc atccatcggg aatactcatt
     1321 tttaattggt aacagcagtt tacctgcttt tatgtcttca acctaccata taggtggcgg
     1381 tggcactggc aatataaagc tgttcttcat tgtgtaattc aacgcgggcg acggcgactt
     1441 tattgcctgc acgcaacagg ctactggtag cagtaaaacg ctcgcccctg cctgggcgca
     1501 gataatcaac gcgaagatca atggtcccca tccgcgatag ccgctggcgt agttcatctt
     1561 cactgatggt ttcgtggcgg gttaaggtac ttcccacgca caccagaccg gcggcgacat
     1621 ccagcgccga cgcaatgacc ccgccgtgca aaatgctttg cgcccagttg cccaccatca
     1681 ttggctgatt tttaaaggcc agctgtgcga actctttttc gtaacgctcc agttccatcc
     1741 ccaatgcgcg gttaaatggc atgtgataaa caaacatctc acccactaat ttcagggctt
     1801 gttcagcggt cagtacggca gacatatcat ccttacactt cattggttaa tgaaatgttg
     1861 attttatgct tctttgttgt tggtttctac tttaggaagg gataactaac ggctatggag
     1921 ttaagtatgt aaaatagccc gcagaaaaat attcacctta tcaataattc gttacggaga
     1981 acacgaccga tgcggactct gcagggctgg ttgttgccgg tgtttatgtt gcctatggca
     2041 gtatatgcac aagaggcaac ggtgaaagag gtgcatgacg cgccagcggt gcgtggcagt
     2101 attatcgcca atatgctgca ggagcatgac aatccgttca cgctctatcc ttatgacacc
     2161 aactacctca tttacaccca aaccagcgat ctgaataaag aagcgattgc cagttacgac
     2221 tgggcggaaa atgcgcgtaa ggatgaagta aagtttcagt tgagcctggc atttccgctg
     2281 tggcgtggga ttttaggccc gaactcggtg ttgggtgcgt cttatacgca aaaatcctgg
     2341 tggcaactgt ccaatagcga agagtcttca ccgtttcgtg aaaccaacta cgaaccgcaa
     2401 ttgttcctcg gttttgccac cgattactgt tttgcaggtt ggacgctgcg cgatgtggag
     2461 atggggtata accacgactc taacgggcgt tccgacccga cctcccgcag ctggaaccgc
     2521 ctttatactc gcctgatggc agaaaacggt aactggctgg tagaagtgaa gccgtggtat
     2581 gtggtgggta atactgacga taacccggat atcaccaaat atatgggtta ctaccagctt
     2641 aaaatcggct atcacctcgg tgatgcggtg ttgagtgcga aaggacagta caactggaat
     2701 accggttacg gcggcgcgga gttaggctta agttacccga tcaccaaaca tgtgcgcctt
     2761 tatactcagg tttacagcgg ctatggcgaa tcgctcatcg actataactt caaccagacc
     2821 cgtgtcggtg taggagttat gctaaacgac ttgttttgat gaacggttga gtggttggca
     2881 aatctggaac ccagcatcca ggattaccct ctcagagact aaaagcattg cagtttctcg
     2941 cgcaggcgct gaaaatagcg cctgttttta tttcaggcaa tcggggtgaa tgtggcgcag
     3001 gcggaagtgt tgaatctgga gtccggagct aaacaggttt tacaagaaac ctttggctac
     3061 caacagtttc gccccggcca ggaagaaatt atcgacactg tgctttccgg tcgcgattgc
     3121 ctggtcgtca tgcccaccgg tggcggaaaa tccctttgct atcaaattcc tgccttattg
     3181 ctaaacggcc ttaccgtggt tgtttcaccg ctgatttcgt tgatgaaaga tcaggtggat
     3241 caactgcaag ccaacggcgt ggcggcggcg tgccttaact cgacgcaaac ccgcgagcag
     3301 caacttgaag tgatgacagg ctgccgcacc gggcaaattc gtctgcttta tatcgccccg
     3361 gaacgcctga tgctggataa ctttcttgag catctggcgc actggaatcc ggtgttatta
     3421 gccgttgatg aagcgcactg tatctcccaa tggggccacg atttccgccc ggaatatgcc
     3481 gcgcttggtc agttacgcca gcgtttcccg accctgccgt ttatggcgct gactgccaca
     3541 gccgacgaca ccacgcgcca ggatatcgtg cgcttgctgg ggctgaacga tccgctgatt
     3601 caaatcagca gttttgaccg tccgaatatt cgctacatgc tgatggagaa attcaaaccg
     3661 ctcgatcagt tgatgcgcta cgtgcaggaa cagcgcggta agtccggcat tatctactgc
     3721 aacagccggg cgaaagtaga agacaccgct gcgcgcctgc aaagcaaggg tattagcgcg
     3781 gcggcctatc atgccgggct ggaaaataat gtccgcgccg acgtgcagga gaaattccag
     3841 cgcgatgacc tgcaaattgt ggtggcgacg gtggcgttcg gcatgggcat caataaacca
     3901 aacgttcgct tcgtggtcca ctttgatatt ccgcgcaata tcgaatccta ttatcaggaa
     3961 accggtcgcg ccgggcgtga tggcctgcct gcggaagcga tgctgtttta cgatccggct
     4021 gatatggcgt ggctgcgccg ttgtctggaa gagaagccgc aggggcagtt gcaggatatt
     4081 gagcgccaca aactcaatgc gatgggcgcg tttgccgaag cgcaaacttg ccgtcgtctg
     4141 gtattgctga actattttgg cgaagggcgt caggagccgt gcgggaactg cgatatctgc
     4201 ctcgatccgc cgaaacagta cgacggttca accgatgctc agattgccct ttccaccatt
     4261 ggtcgtgtga atcagcggtt tgggatgggt tatgtggtgg aagtgattcg tggtgctaat
     4321 aaccagcgta tccgcgacta tggtcatgac aaactgaaag tctatggcat gggccgtgat
     4381 aaaagccatg aacattgggt gagcgtgatc cgccagctga ttcacctcgg cctggtgacg
     4441 caaaatattg cccagcattc tgccctacaa ctgacagagg ccgcgcgccc ggtgctgcgc
     4501 ggcgaatcct ctttgcaact tgccgtgccg cgtatcgtgg cgctcaaacc gaaagcgatg
     4561 cagaaatcgt tcggcggcaa ctatgatcgc aaactgttcg ccaaattacg caaactgcgt
     4621 aaatcgattg ccgatgaaag taatgtcccg ccgtacgtgg tgtttaacga cgcaaccttg
     4681 attgagatgg ctgaacagat gccgatcacc gccagcgaaa tgctcagcgt taacggcgtt
     4741 gggatgcgca agctggaacg ctttggtaaa ccgtttatgg cgctgattcg tgcgcatgtc
     4801 gatggcgacg acgaagagta gtcagcagca taaaaaagtg ccaggatgaa gactccgtaa
     4861 acgtttcccc cgcgagtcaa atgtatgttg atgttatttc tcaccgtcgc catggtgcac
     4921 attgtggcgc ttatgagccc cggtcccgat ttcttttttg tctctcagac cgctgtcagt
     4981 cgttcccgta aagaagcgat gatgggcgtg ctgggcatta cctgcggtgt aatggtttgg
     5041 gctgggattg cgttgcttgg cctgcatttg attatcgaaa aaatggcctg gctgcatacg
     5101 cttatggttg gcggtggcct gtatctctgc tggatgggtt accagatgct gcgcggcgcg
     5161 ctgaaaaaag aggcggtttc tgcacctgcg ccacaggtcg agctggcgaa aagtgggcgc
     5221 agtttcctga aaggtttact gaccaatctc gctaatccga aagcgattat ctactttggc
     5281 tcggtgttct cattgtttgt cggtgataac gttggcacta ccgcgcgctg gggcattttt
     5341 gcgctgatca ttgtcgaaac gctggcgtgg tttatcgtcg ttgccagcct gtttgccctg
     5401 ccgcaaatgc gccgtggtta tcaacgtctg gcgaagtgga ttgatggttt tgccggggcg
     5461 ttatttgccg gatttggcat tcatttgatt atttcgcggt gatgccagac gcgtcttcag
     5521 agtaagtcgg ataaggcgtt tacgccgcat ccgacattat ttttcacgca tgcctcgccg
     5581 atgctaacag cgctcccacc agcataaaca acgagccgaa aatcttattc agcgccttca
     5641 tctgctttgg tcctttaatc catagagcaa tccgttgagc aagggtggcg taaccgatca
     5701 tcacaataat atcgaccaca atagtggtga cgccgagcac gatatactgc atcagttgcg
     5761 gctgttgcgg catgatgaat tgcggaaata gcgccgccag aaacacaata cttttgggat
     5821 tggtgagatt cacaaaaact gcgcgctgga acaaatgtcg acgcgattga gtagaggcca
     5881 gcgatttaag gtcaattgca ccagcggcgc gccactgctg gattcccagc caaatcaagt
     5941 aagccgcgcc tgcccacttc aacacttcaa acgcaatcac tgagcgggaa aatagcgtcc
     6001 ccaaccccac gccaaccagc acaatatgaa tcgccagtcc ggtctgaagc ccagcaatag
     6061 acgccaccgc gccgcgataa ccgtggttga gcgaggtggt catagtgttg attgcaccag
     6121 agcctggcga caggcttaaa atgatcgatg tcagcaggta ggcaaaccac cattctaagg
     6181 tcatgatgaa ctcccggtgt gtctattttt gtgccacaat acgctactgt cgcagcgttg
     6241 tgtcaggcac gctaaaaaaa acgattttac gtggtttaag aggcagatta cccgatgttt
     6301 cagcagcaaa aagactggga aacaagagaa aacgcgtttg ctgcttttac catgggaccg
     6361 ctgactgatt tctggcgtca gcgtgatgaa gcagagttta ctggtgtgga tgacattccg
     6421 gtgcgctttg tccgttttcg cgcacagcac catgaccggg tggtagtcat ctgcccgggg
     6481 cgtattgaga gctacgtaaa atatgcggaa ctggcctatg acctgttcca tttggggttt
     6541 gatgtcttaa tcatcgacca tcgcgggcag ggacgttccg gtcgcctgtt agccgatccg
     6601 catctcgggc atgttaatcg ctttaatgat tatgttgatg atctggcggc attctggcag
     6661 caggaggttc agcccggtcc gtggcgtaaa cgctatatac tggcacattc gatgggcggt
     6721 gcgatctcca cattatttct gcaacgccat ccaggtgtat gtgacgccat tgcgctaact
     6781 gcgccaatgt ttgggatcgt gattcgtatg ccgtcattta tggcacggca gatcctcaac
     6841 tgggccgaag cgcatccacg tttccgtgat ggctatgcaa taggcaccgg gcgctggcgc
     6901 gcgttgccgt ttgctatcaa cgtactgacc cacagcagac agcgatatcg acgtaactta
     6961 cgcttctatg ctgatgaccc aacgattcgc gtcggtgggc cgacctacca ttgggtacgc
     7021 gaaagtattc tggctggcga acaggtgtta gccggtgcgg gtgatgacgc cacgccaacg
     7081 cttctcttgc aggctgaaga ggaacgcgtg gtggataacc gcatgcatga ccgtttttgt
     7141 gaactccgca ccgccgcggg ccatcctgtc gaaggaggac gaccgttggt aattaaaggt
     7201 gcttaccatg agatcctttt tgaaaaggac gcaatgcgct cagtcgcgct ccacgccatt
     7261 gttgattttt tcaacaggca taactcaccc agcggaaacc gctctacaga ggtttaaatt
     7321 tcttatgtac caggttgttg cgtctgattt agatggcacg ttactttctc ccgaccatac
     7381 gttatcccct tacgccaaag aaaccctgaa gctgctcacc gcgcgcggca tcaactttgt
     7441 gtttgcgacc ggtcgtcacc acgttgatgt ggggcaaatt cgcgataatc tggagatcaa
     7501 gtcgtacatg atcacctcca acggcgcgcg cgttcacgat ctggatggca atctgatttt
     7561 tgctcataac ctggatcgcg acattgccag cgatctgttt ggcgtagtca acgacaatct
     7621 ggacatcatt actaacgttt atcgcgacga cgaatggttt atgaatcgcc atcgcccgga
     7681 agagatgcgc ttttttaaag aagcggtgtt caaatatgcg ctgtatgagc ctggattact
     7741 ggagccggaa ggcgtcagca aagtgttctt cacctgcgat tcccatgaac aactgctgcc
     7801 gctggagcag gcgattaacg ctcgttgggg cgatcgcgtc aacgttagtt tctctacctt
     7861 aacctgtctg gaagtgatgg cgggcggcgt ttcaaaaggc catgcgctgg aagcggtggc
     7921 gaagaaactg ggctacagcc tgaaggattg tattgcgttt ggtgacggga tgaacgatgc
     7981 cgaaatgctg tcgatggcgg ggaaaggctg cattatgggc agtgcgcacc agcgtctgaa
     8041 agaccttcat cccgagctgg aagtgattgg tactaatgcc gacgacgcgg tgccgcatta
     8101 tctgcgtaaa ctctatttat cgtaatcgtt ctttatttgg tcagttgtca acctgatact
     8161 tcgctacaat ggatactcgt taatcaaaga gttttccatt gtggcgctac ttatcatcac
     8221 cacgattctg tgggccttct cctttagctt ttatggcgag taccttgcgg ggcacgtcga
     8281 tagctatttt gcggtgctgg tgcgcgttgg cctggcggca ctcgtttttc tgccgtttct
     8341 gcgtacccgt ggcaatagcc tgaaaacggt cggcctgtat atgctggtgg gcgcgatgca
     8401 gctcggcgtg atgtatatgc tgagtttccg cgcttatctc tacctgacgg tctccgaatt
     8461 gttgctgttc accgtactga cgccgctcta catcacgctg atttatgaca tcatgagtaa
     8521 gcgccgtctg cgctggggct atgcttttag cgccttgctg gcggtgattg gtgccgggat
     8581 tattcgctat gatcaggtta ccgaccattt ctggactggc ttgttgctgg tgcaactctc
     8641 caatatcact tttgccatcg gcatggtggg ttacaaacgc ctgatggaaa ctcgcccgat
     8701 gccgcagcat aacgcctttg cgtggttcta tcttggcgcg ttcctggtgg cggtaattgc
     8761 atggttcttg ctgggaaatg cgcagaaaat gccgcaaacc acgttgcaat ggggcattct
     8821 ggtgtttctt ggcgtggtgg cttccgggat tggctacttt atgtggaact acggcgcgac
     8881 gcaggtggac gccggaacgc tgggcattat gaataatatg cacgttccgg cagggctgct
     8941 ggtaaacctg gctatctggc accaacagcc gcactggcca acgttcatta caggcgcgct
     9001 ggtgatcctg gcctcactgt gggtgcatcg taagtgggtc gctccgcgct cttcacaaac
     9061 ggcagatgat cgcaggcgtg attgcgcgct gagcgaataa atgcttctgt aatcggctga
     9121 cgctgctcgc catcgcgcac ggcggcgtac agtcggctcc acaagccttc gcccagggtt
     9181 ttggtcacca ccagaccctg gcgctcaaaa ctctctacta cccaatgcgg tagcgcggca
     9241 atacccatcc gcgcggcaac catctgaatc aacaataagg tgttatcgac gcttttcagt
     9301 gacgggctga cgcctgccgg ctgaagaaaa tgccgccaga catccagtcg actacgctgc
     9361 accggataaa tcaacagcgt ctcgctggcg agatcttccg gtgtaattcg cgttttcgcc
     9421 gccagtggat ggtcaggtgc taacaccaga cgcacttcat agtcgaacat cggcgaataa
     9481 tgcaggccac tgcgcggcag gatatcggag gtcatcacca gatccagctc gccctgttgc
     9541 aacgcaggct gcgggtcaaa cgtcacgccc gatttaaaat ccatctctac ctgcggccag
     9601 ttcttatgga aattttctaa cgcgggcgtc agccactgaa tacagctatg gcactcaatg
     9661 gcaatgcgca gacgcgtctg ctgcggttca ttgcaggctt gcagtgcctg gctgatttgc
     9721 ggcagcacct ggtttgccag ttgcaacagg atttctccct gcggtgtaaa gcgtagcggc
     9781 tggctcttac gcacaaatag ccggaagcca aggcgttgtt ccagatcgct aaactggtga
     9841 gacagggcgg attgcgtctg atgcaacgtc gccgcagcgg ctgcgagaga gccgcagttc
     9901 cgcaacgctt gtagcgtttt caggtgtttt acttcgatca tgaaagtcct tcacttcggc
     9961 atgaataatt tgcgcttgag gaatatacag taaccgccaa ttatggatgt gtaaacatct
    10021 ggacggctaa aatccttcgt cttttaaatt tatggtgcgt tggctgcgtt tctccacccc
    10081 ggtcacttac ttcagtaagc tcccggggat gaataaactt gccgccttcc ctaaattcaa
    10141 aatccatagg atttacatat aattagagga agaaaaatga caatattgaa tcacaccctc
    10201 ggtttccctc gcgttggcct gcgtcgcgag ctgaaaaaag cgcaagaaag ttattgggcg
    10261 gggaactcca cgcgtgaaga actgctgaca gtagggcgtg aactgcgtgc tcgtcactgg
    10321 gatcaacaaa agcaagcggg tatcgacctg ctgccggtgg gcgattttgc ctggtacgat
    10381 catgtactga ccaccagttt gctgctgggt aatgttccgc cacgtcatca gaacaaagac
    10441 ggttcggtag atatcgacac cctgttccgt attggtcgtg gacgtgcgcc gactggcgaa
    10501 cctgcggcgg cagcggaaat gaccaaatgg tttaacacca actatcacta catggtgccg
    10561 gagttcgtta aaggccaaca gttcaaactg acctggacgc agctgctgga ggaagtggac
    10621 gaggcgctgg cgctgggcca caacgttaag cctgtgctgc tggggccggt tacttacctg
    10681 tggctgggga aagtgaaagg tgaacagttt gaccgcctta gcctgctgaa cgacattctg
    10741 ccggtttatc agcaagtgct ggcagaactg gcgaaacgcg gcatcgagtg ggtacagatt
    10801 gatgaacccg cgctggtact ggaactacca caggcgtggc tggacgcata caaacccgct
    10861 tacgacgcgc tccagggaca ggtgaaactg ctgctgacca cctattttga aggcgtaacg
    10921 ccaaatctcg acacgattac tgcgctgcct gttcagggtc tgcatgttga tctcgtacat
    10981 ggtaaagatg acgttgctga actgcacaag cgcctgcctt ctgactggtt gctgtctgcg
    11041 ggtctgatca atggtcgtaa cgtctggcgc gccgatctta ccgagaaata tgcgcaaatt
    11101 aaggacattg tcggcaaacg tgatttgtgg gtggcatctt cctgctcgtt gctgcacagc
    11161 cccatcgacc tgagcgtgga aacgcgtctt gatgcagaag tgaaaagctg gtttgccttc
    11221 gccctacaaa aatgccatga actagcactg ctgcgcgatg cgctgaacag tggtgacacg
    11281 gcagctctgg cagagtggag cgccccaatt caggcgcgtc gtcactctac tcgtgtacat
    11341 aatccggcgg tagaaaagcg tctggcggcg atcaccgccc aggacagcca gcgtgcgaat
    11401 gtctatgaag tgcgtgctga agcccagcgt gcacgtttta aactgccagc gtggccgacc
    11461 accacgattg gttccttccc gcaaaccacg gaaattcgta ccctgcgtct ggatttcaaa
    11521 aagggcaatc tcgacgccaa taactaccgc acgggcattg cggaacatat caggcaggcc
    11581 attgttgaac aggaacgttt gggactggat gtgctggtac atggcgaggc cgagcgtaat
    11641 gacatggtgg aatactttgg cgagcacctc gacgggtttg tctttacgca aaacggttgg
    11701 gtacagagct acggttcccg ctgcgtgaag ccaccgattg ttattggtga cgttagtcgc
    11761 ccggcaccga ttaccgtgga gtgggcgaag tatgcgcaat ctctgactga taaaccggtg
    11821 aaagggatgc tgactggccc ggtgactatt ctctgctggt cgttcccgcg tgaagatgtc
    11881 agccgtgaaa ccatcgccaa acagattgcg ctggcgctgc gtgatgaagt ggccgatctg
    11941 gaagccgctg gaattggcat catccagatt gacgaaccgg ctctgcgcga aggtttaccg
    12001 ctgcgtcgta gcgactggga tgcgtatctc cagtggggcg tagaggcctt ccgtatcaac
    12061 gccgccgtgg cgaaagatga cacacaaatc cacactcaca tgtgttattg cgagttcaac
    12121 gacatcatgg attcgattgc ggcactggac gcagacgtca tcaccatcga aacctcgcgt
    12181 tctgacatgg agttgctgga gtcgttcgaa gagtttgatt atccgaatga aatcggtcca
    12241 ggcgtctatg acattcactc gccaaacgta ccgagcgtgg aatggattga agccttgctg
    12301 aagaaagcgg caaaacgcat tccggcagag cgcctgtggg ttaacccgga ctgtggcctg
    12361 aaaacgcgcg gctggccaga aacccgcgcg gcactggcga acatggtgca ggcggcgcag
    12421 aatttgcgtc ggggatgatg aatattaacg agtcactgta aattgcagta cgatgaaaga
    12481 ggcaaaactg cctgatacta ggcctacata atttatgcaa tttattgaat ttttgggttt
    12541 tgttggccgg acaaggcgtt cacgccgcat ccggcatgaa caacgcgcac tttgccaata
    12601 atctgatggc ttccatcacc ggaagccatt ttgctatctg cgcccgttca gatacagaaa
    12661 taaccgaatc atttttacgc aaaatataat cctgagcgat ttgttcagta atcaacgcta
    12721 cgttatttat tctcactttt cacgaaattg tatccctttt cagtaacgtg gtagtgaggc
    12781 atagacttat gtccgctatc ctcaataata accaaaccct cgttagctaa tttgttaaca
    12841 atgttaacaa cattaccgaa tcctggaatt tgccttatgg atagtgtgcg atcgagattc
    12901 atccagttcc agtttatatc tttctcagcc ataattttca gtacgttaat ttcagtttca
    12961 gttatcatag tcaagtgcat cctggtggta taagtcatta agttctccct taagtttata
    13021 atcttctaga gtagcagtat gcgta
//
LOCUS       DAZOZY010000113        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZOZY010000113 DAZOZY010000000
VERSION     DAZOZY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.113"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHURQ_002746"
     CDS             <1..191
                     /locus_tag="ACHURQ_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824481.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHURQ_002747"
     CDS             191..1393
                     /locus_tag="ACHURQ_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR0824482.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHURQ_002748"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHURQ_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR0824483.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHURQ_002749"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHURQ_002749"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR0824484.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHURQ_002750"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHURQ_002750"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR0824485.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHURQ_002751"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHURQ_002751"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR0824486.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHURQ_002752"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHURQ_002752"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR0824487.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHURQ_002753"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHURQ_002753"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR0824488.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHURQ_002754"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHURQ_002754"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR0824489.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZOZY010000114        12592 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZOZY010000114 DAZOZY010000000
VERSION     DAZOZY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.114"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHURQ_002755"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHURQ_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR0824490.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHURQ_002756"
     CDS             433..621
                     /locus_tag="ACHURQ_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR0824491.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHURQ_002757"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHURQ_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR0824492.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHURQ_002758"
     CDS             816..989
                     /locus_tag="ACHURQ_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR0824493.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHURQ_002759"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHURQ_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHURQ_002760"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHURQ_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR0824494.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHURQ_002761"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHURQ_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR0824495.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHURQ_002762"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHURQ_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR0824496.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHURQ_002763"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHURQ_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR0824497.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHURQ_002764"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHURQ_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHURQ_002765"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHURQ_002765"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR0824498.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHURQ_002766"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHURQ_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR0824499.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHURQ_002767"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHURQ_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR0824500.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3048 a         3214 c         3289 g         3041 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgtggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aa
//
LOCUS       DAZOZY010000115        12563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZOZY010000115 DAZOZY010000000
VERSION     DAZOZY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.115"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHURQ_002768"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHURQ_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR0824501.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHURQ_002769"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHURQ_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR0824502.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHURQ_002770"
     CDS             1797..1970
                     /locus_tag="ACHURQ_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824503.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHURQ_002771"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHURQ_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR0824504.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHURQ_002772"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHURQ_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR0824505.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHURQ_002773"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHURQ_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR0824506.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHURQ_002774"
     CDS             complement(4268..5191)
                     /locus_tag="ACHURQ_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0824507.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="ACHURQ_002775"
     CDS             5408..6709
                     /locus_tag="ACHURQ_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0824508.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="ACHURQ_002776"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="ACHURQ_002776"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR0824509.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="ACHURQ_002777"
     CDS             8771..8995
                     /locus_tag="ACHURQ_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR0824510.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="ACHURQ_002778"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="ACHURQ_002778"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR0824511.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHURQ_002779"
     CDS             complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHURQ_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR0824512.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="ACHURQ_002780"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="ACHURQ_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR0824513.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="ACHURQ_002781"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="ACHURQ_002781"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR0824514.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2927 c         3146 g         3282 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 catattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAZOZY010000116        12300 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZOZY010000116 DAZOZY010000000
VERSION     DAZOZY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12300
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.116"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /gene="ydjO"
                     /locus_tag="ACHURQ_002782"
     CDS             complement(<1..264)
                     /gene="ydjO"
                     /locus_tag="ACHURQ_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFR0824515.1"
                     /translation="MGREMFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFIS
                     TGQCDCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQD"
     gene            complement(541..804)
                     /gene="cedA"
                     /locus_tag="ACHURQ_002783"
     CDS             complement(541..804)
                     /gene="cedA"
                     /locus_tag="ACHURQ_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR0824516.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            987..3248
                     /gene="katE"
                     /locus_tag="ACHURQ_002784"
     CDS             987..3248
                     /gene="katE"
                     /locus_tag="ACHURQ_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR0824517.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3506..4264)
                     /gene="chbG"
                     /locus_tag="ACHURQ_002785"
     CDS             complement(3506..4264)
                     /gene="chbG"
                     /locus_tag="ACHURQ_002785"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR0824518.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(4277..5629)
                     /gene="celF"
                     /locus_tag="ACHURQ_002786"
                     /gene_synonym="chbF"
     CDS             complement(4277..5629)
                     /gene="celF"
                     /locus_tag="ACHURQ_002786"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR0824519.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5734..6576)
                     /gene="chbR"
                     /locus_tag="ACHURQ_002787"
     CDS             complement(5734..6576)
                     /gene="chbR"
                     /locus_tag="ACHURQ_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR0824520.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6584..6934)
                     /gene="chbA"
                     /locus_tag="ACHURQ_002788"
     CDS             complement(6584..6934)
                     /gene="chbA"
                     /locus_tag="ACHURQ_002788"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR0824521.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6985..8343)
                     /gene="chbC"
                     /locus_tag="ACHURQ_002789"
     CDS             complement(6985..8343)
                     /gene="chbC"
                     /locus_tag="ACHURQ_002789"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR0824522.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(8428..8748)
                     /gene="chbB"
                     /locus_tag="ACHURQ_002790"
     CDS             complement(8428..8748)
                     /gene="chbB"
                     /locus_tag="ACHURQ_002790"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR0824523.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(9047..9385)
                     /gene="osmE"
                     /locus_tag="ACHURQ_002791"
     CDS             complement(9047..9385)
                     /gene="osmE"
                     /locus_tag="ACHURQ_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR0824524.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9587..10414
                     /gene="nadE"
                     /locus_tag="ACHURQ_002792"
     CDS             9587..10414
                     /gene="nadE"
                     /locus_tag="ACHURQ_002792"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR0824525.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10644..11531
                     /gene="cho"
                     /locus_tag="ACHURQ_002793"
     CDS             10644..11531
                     /gene="cho"
                     /locus_tag="ACHURQ_002793"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR0824526.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11491..12066)
                     /gene="ves"
                     /locus_tag="ACHURQ_002794"
     CDS             complement(11491..12066)
                     /gene="ves"
                     /locus_tag="ACHURQ_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR0824527.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         3296 a         3126 c         2777 g         3101 t
ORIGIN      
        1 gtcctgcaga tacgtatcaa aactaagtgc tggagctcct ggctttgagg catcatagtt
       61 aatgttagac atataggcaa tcatacgatt gaagttcgac tctgctattt catcgcagtc
      121 acattgaccc gttgatataa agaaagtgag tgccgccatg gtagactcaa gcctttcttt
      181 aaaagagtcg aaagccaggc ttttaatatt taaatcacca taattactct gtattaagtt
      241 tgtagaaaac atctcccgcc ccatattgtt aacaaaatta ttatctcatt taaatctaag
      301 tcacttacaa tataagttta agagcgacgc cacaggatga actatcaaaa atagctcatc
      361 atgattagca aaacttaacc attttaaaat aaataaacaa ttaaagaaaa aagatcactt
      421 atttatagca atagctcgtc aaaggcagct ttttgttaca ggtggtttga atgaatgtag
      481 caacgaaatg cagaatttca ggtcatgtaa ctcccggcaa aaccgggaag tatgtaatcc
      541 ttactcagtc acttcccctt cctggcggat ctgatttgcc caacgttggg cagattcagg
      601 cacagtaaac gccggtgagc gcagaaatga ctctcccatc agtacaaacg caacatattt
      661 gccacgcagc atccagacat cacgaaacga atccatcttt atcgcatgtt ctggcggcgc
      721 gggttccgtg cgtgggacat agctaataat ctggcggttt tgctggcgga gcggtttctt
      781 cattactggc ttcactaaac gcatattaaa aattagaaaa actgtagttt agccgattta
      841 gcccctgtac gtcccgcttt gcgtgtattt cataacaccg tttccagaat agtctccgaa
      901 gcgggatctg gctggtggtc tatagttaga gagttttttg accaaaacag cggccctttc
      961 agtaataaat taaggagacg agttcaatgt cgcaacataa cgaaaagaac ctacatcagc
     1021 accagtcacc actacacgat tccagcgaag cgaaaccggg gatggactca ctggcacctg
     1081 aggacggatc tcatcgtcca gcggctgaac caacaccgcc aggtgcacaa cctaccgccc
     1141 ctgggagcct gaaagcccct gatacacgta acgaaaaact taattctctg gaagacgtac
     1201 gcaaaggcag tgaaaattat gcgctgacca ctaatcaggg cgtgcgcatc gccgatgatc
     1261 aaaactcact gcgtgccggt aaccgtggtc caacgctgct ggaagatttt attctgcgcg
     1321 agaaaatcac ccactttgac catgagcgca tcccggaacg tattgttcat gcacgcggat
     1381 cagcagctca cggttatttc cagccatata aaagcttaag cgatatcacc aaagcggatt
     1441 tcctctcaga tccgaacaaa atcaccccag tatttgttag gttctctacc gttcagggtg
     1501 gcgctggctc tgcagatacc gtgcgtgata tccgtggttt tgccaccaag ttctatactg
     1561 aagagggtat ttttgacctc gttggcaata acacgccaat cttctttatc caggatgcgc
     1621 ataaattccc cgattttgtt catgcggtaa aaccagaacc gcactgggca attccacaag
     1681 ggcaaagtgc ccacgatact ttctgggatt atgtttctct gcaacctgaa actctgcaca
     1741 acgtgatgtg ggcgatgtcg gatcgcggca tcccccgcag ttaccgcacc atggaaggct
     1801 tcggtattca caccttccgc ctgattaatg ccgaagggaa ggcaacgttt gtacgtttcc
     1861 actggaaacc actggcaggt aaagcctcac tcgtttggga tgaagcacaa aaactcaccg
     1921 gacgtgaccc ggacttccac cgccgcgagt tgtgggaagc cattgaagca ggcgattttc
     1981 cggaatacga actgggcttc cagttgattc ctgaagaaga tgaattcaag ttcgacttcg
     2041 atcttctcga tccaaccaaa ctgatcccgg aagaactggt gcccgttcag cgtgtcggca
     2101 aaatggtgct caatcgcaat ccggataact tctttgccga aaacgaacaa gcagcattcc
     2161 atccaggtca tattgttcct ggtctggact tcaccaacga tccgctgttg cagggacgtt
     2221 tgttctccta taccgataca caaatcagtc gtcttggtgg gccgaatttc catgagattc
     2281 cgattaaccg tccgacctgc ccttaccaca acttccagcg tgatggcatg catcgtatgg
     2341 ggatcgacac taacccggcg aattacgaac cgaactcgat caacgataac tggccgcgcg
     2401 aaacaccgcc gggaccgaaa cgcggcggtt ttgaatcata tcaggaacgc gtggaaggaa
     2461 ataaagttcg tgagcgcagt ccatcgtttg gcgaatatta ttcccatccg cgtctgttct
     2521 ggctaagtca gacgccattc gagcagcgcc atattgtcga tggtttcagt tttgagttaa
     2581 gcaaagtcgt tcgtccgtat attcgtgagc gcgttgttga ccagctggcg catattgatc
     2641 tcactctggc ccaggcggtg gcgaaaaatc tcggtatcga actgactgac gaccagctga
     2701 atatcacccc acctccggac gtcaacggtc tgaaaaagga tccatcctta agtttgtacg
     2761 ccattcctga cggtgatgtg aaaggtcgcg tggtagcgat tttacttaat gatgaagtga
     2821 gatcggcaga ccttctggcc attctcaagg ccctgaaggc caaaggcgtt catgccaaac
     2881 tgctctactc ccgaatgggt gaagtgactg cggatgacgg tacggtgttg cctatagccg
     2941 ctacctttgc cggtgcacct tcgctgacgg tcgatgcggt cattgtccct tgcggcaata
     3001 tcgcggatat cgctgacaac ggcgatgcca actactacct gatggaagcc tacaaacacc
     3061 ttaaaccgat tgcgctggcg ggagacgcgc gcaagtttaa agcaacaatc aaggtcgctg
     3121 accagggtga agaagggatt gtggaagctg acagcgctga cggtagtttt atggatgaac
     3181 tgctaacgct gatggcagca caccgcgtgt ggtcacgcat tcctaagatt gacaaaattc
     3241 ctgcctgatg ggaggcggcg caattgcgcc gcctcaaatg atttacatag tgcgctttgt
     3301 ttatgccgga tgcggcgtga acgccttatc cggcctacaa aactgtgcaa attcaatata
     3361 ttgcaggaaa cacgtaggcc tgataagcga agcgcatcag gcagttttgc gtttgtcagc
     3421 agtctcaagc ggcgcagtta cgccgccttt gtaggaatta atcgccggat gcaaggttca
     3481 cgccgcatct ggcaaacatc ctcacttaca catcccgata actccccaac cgataaccac
     3541 gctgagcgat agcacctttc aacgacgctg atgtcaacac atccagctcc gttaagcgtg
     3601 ggaaacagta agcactctga cggatagtat tatcgataaa cgccggatga cacatcacct
     3661 ccagcgaacg atcaccccga tggcttgcgt catccagcac ttgcaggaac agtgcctcgc
     3721 taatctcttc accataaaac gcgctactga atccctgaga actgcgcaga ttagccggta
     3781 aatcaccaga gttagatagc ggctgacggt cgatgcgcaa cgcaatcccc tcttcagccg
     3841 caaacctggc aacaatcggg aaaatctgcg ggaacatatg cacatgatga tggctatcaa
     3901 gatgcgtagg tttgcgtcca aatagctcaa tgaaacgcaa atactgactg gcaagctcct
     3961 gagtaatttc ttccagcggt aaagcatctt cttctgccaa ctgccagatc cattttccca
     4021 gcacaccatc gcgggttaac cccggcatag ctgtcagtgg cttgcccata gtaaggacaa
     4081 agtacatccc tatggccaga ctcggttcat cacgactcaa ctgcaccgca tggtcaatag
     4141 cctgcccatt caccagcgcc gtcgtcgatg tgacaatccc attgcgacag gcctcgataa
     4201 tgccgtagtt ctgacctttg cttaagccaa aatcatcggc attaacaatc agtaagcgtt
     4261 ccataatcag cctcggttaa tgtgcttttt taagctctgc gatgcagtcg gcaaaatttg
     4321 gcagccattt ctcgtgcgcc agaatcatct cgcgtgccag cagctcagca tcgcgatcgg
     4381 aatgcaccaa cggactaagg tttagcgcca gtaacacatc gttaaattct ccgctaagtg
     4441 cggcgttgct ggcagcaatc tcgaagcctt taatggtgtg aatcagcccc atcactttat
     4501 catcgaaatg cgtaatgcgc ggatgtggcg tcgcgccatc gcgccccagc ttacaggtca
     4561 tttctaccgc ccagtctgcc ggaatattat caatatgccc atgatgcggg atattaacgt
     4621 aatgttcagc ttgcttgtcg ttgtagatag cgttgatcac ttcgcacgct gcatcagagt
     4681 aataagcccc accgcgctgt tccagttctt tcggcttaac tttcaactcc gggtttttat
     4741 acagctcaaa aagttgtttc tcgactttct gtactacctg cgctcgtgcg ccgcctttgt
     4801 agtattcgcc catttcaata gccagcatct ctttttgctt gaagtaataa agcaaataag
     4861 aacacggcaa cagattcaga gaacgaatta agccttcgct aaatggcaga tcgaaaatat
     4921 ttttaacgcc agatgctttt aactgcccgg atgccacacc atcaagcaat tcggcaaagc
     4981 gcgacttgcc atttaccagc acatccttaa tgaacaccat atggttgagg ccgaacaaat
     5041 cgatagataa atcatcgctg tctttcagca tcagaacatc gtgaataaac atcttcatgc
     5101 cgatcggaat attacacacg ccgataaagc gtttaaatcc ggtatgacga taaacggctt
     5161 cagtaaccat tcccgccggg ttagtgaagt taatcaccca tgcattcgga caaagttctt
     5221 cgacatcttt tacgatgtca aaaatcaccg gaatggtacg cagaccttta aacagaccgc
     5281 ccgcgccgtt ggtttcctgg ccaagataac catgacttaa tggaatacgt tcatccagtt
     5341 cgcgcgccgg taattggcct acgcgcagtt gggtagtaac gaaatcagca tctttcaatg
     5401 cttcgcggcg atccagcgtt ttataaagct tcatcgggac gccagcgtta tcaatcatcc
     5461 gttggcagag ttcaaaaatg atatccagtt tctctttacc atcttcgaca tccaccagcc
     5521 ataattcgct gaccggcaat tcgtgataac gcttaataaa tccttccagt aactccgggg
     5581 tatagctgct cccgccacca atagtgacga cttttaattt ctggctcata atttctccct
     5641 tcagtacaga atactgatat ctggcatatc tgcccccccg gacataaata atccagcaac
     5701 aggacagata tgtgaattgt caggtataac gacttactga ttaaattcag tcaatttctt
     5761 acgatagctc ttaggcgtaa aggacgttaa tttcttaaac gttttaataa acaaactcgg
     5821 gctactataa ccggcctcaa acgcaatatc cgtcacggaa tagttggtca tttccagttg
     5881 ttttttggca aaattaatac ggatctcatt aataatctgc attggtgttt tgccataata
     5941 tcgttgagtc gctcgcgtca aatattcctg tgatttggct gacaacgtca ccatattctc
     6001 cagcgccgat tcactaaact gctctttatc atgcatcttt tctaccgtac ttttcagcca
     6061 ctgcggtaca tcatcaatca cctgttcttc gcggtaatga cgtaaacggt taatgacata
     6121 aaaagtaacc atctcaacaa actcttccag ccctgtttcg cggaaattca atgaagaaat
     6181 cactgtttcc acataggtga gaaaagcgtt attggtccgg tatacctgcg aagcgacaaa
     6241 gcaataaggc aacaacggca ggtaatgctg ctcaaaaaag cgtttactga tcccaacgtt
     6301 caatatacgc gtggcaccaa actcataaaa actttgatgg tgcgaaccta acggaataaa
     6361 aacaaaatcg ccccgttcca gtaacacgcg cttaccgtta atctcctgga aataacgccc
     6421 ggttaatacc agagtaaatt cataatagtc gtgctgatgc agcccgctga tactctcagt
     6481 tttgttatag ataaacacat ggaagttttt gccattaaac aactgctgtt ctcgggcagt
     6541 ggcaatttcc ggcgcgttaa tcactggctg catcatcgac tccttatgcc ttcagttttt
     6601 catgaagctc aattaattca gtaatcagtt cacgcgcaag catggacgtc attaaatgat
     6661 cctgagcgtg gaccagcacc agactcactt tcatcttacc ttcgcccgca tcgccttcaa
     6721 tcagtttcgt ctgtaccaga tgcgcttcat tcaatgccat ccgtgactga tccatcatgg
     6781 cttttgctgc agcaaaatcg ccctgcttcg cctgtttcag tgccgcatac gccaggctgc
     6841 gcgcttgtcc ggagttgatg ataagcccca tcaccacttc ttccagctct tcagcttccg
     6901 tttgcgtatc gggaatgtta tcgagatcca tcatacatcg ttcctctcta ttcttaccgg
     6961 cacgattacc cgtaccggca tcgattaaaa tttcagggcg ttagcgatat cttcttcgcg
     7021 ctcttcttta tcaatcgcgt tttgtgcttt gttagccacc acaacaaagg gcagataaat
     7081 taacgttgcg ataccaaggt tgaagagtgc aaccagcaat gcggcgacgc taccgttggt
     7141 gttaaagaag gctcccagac cggttggcat ggtccacggt gcaatattgg tcaccggagg
     7201 aatgatgccc atgtagtacg ctgcgagggt gattgccgcc agaatcggtt gtaccagtac
     7261 aaacgggata aacatcaccg ggttcatgat aattggcaga ccaaacagaa tcggttcgtt
     7321 aatctggaag atgccggacg gcagcgccag ttttgccacc tgacgataat cagcacggcg
     7381 agaggcgata aagatagcca ggatcaggcc taaagtcgca ccactgcccc caaggaaaat
     7441 aaaggagtcc agcatcggct tggcccagat atggaaggtc ttaccggctg ccagcgccgc
     7501 ttcgacggaa ccatattgct gataggtcgc gatattttcc agcgcccacg gcgtcataat
     7561 gccgttgtcc agtgcggtca gcgccagcgc gccatgaata ccgaagaacc agagcagtgg
     7621 aacaaagatc acataggccc agcccaccac gctacccaac gatgccagtg gggttgagat
     7681 ggtatccata atgatctgat ggaagttggt gccccaggta ttcaacgccc aggcaataat
     7741 ccccatcacg gaaagaataa taaagccggg aattaatgcc gagaaggaac gcgatactga
     7801 agcaggtacg ctatcgggca atttaatgac ccaattgcgg cggacaataa aggtaaacat
     7861 ttctgccacc accaggccaa taataatccc ggagatgata ttcgctccac ctaaccagtt
     7921 tgcgccaacc acataggcct caccgacact atatggggtg acggtcataa atgctgcaac
     7981 ggataacaac ccagccgcca gcgcatcgac tttacgctct tctgccagcg ccatgccaat
     8041 aaagaacggt gccattaaag acattatccc taatgttccg ttatatacgt tgccgccaat
     8101 acctttcagg ccattaagtg tttcaatggt tgaggcgtcg aggcgaatac ctaaggaata
     8161 aaaaaacgcc ccctccccaa agcttagaaa aacgttgtta attaatacaa acatggcccc
     8221 cgcaagggtt aacggcatta agcgaataaa gccatttttg attgcattaa cgtgtggctg
     8281 ctttcctatt ttaactgcaa aagggaggag taccttttca agcgatgcaa taacattact
     8341 catagaaaaa tacccttaaa aaccgcaatt taaatattgc ggtattgatt tatgaaataa
     8401 ctctttgacg ggaaaattta aaataaatta atttgctgcg gcttttttaa tcgctgcaac
     8461 cgcagcctta agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc
     8521 aaccggtttg ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc
     8581 taataacacg acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc
     8641 aataatgacc ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga
     8701 ggtagacatg cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc
     8761 atcgacgatt atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg
     8821 taacttgcga aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg
     8881 agcaatagta tttctttgac cttataaatt gtttctcctt gacctgtggt tatgaccctc
     8941 ttcacatttc gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg
     9001 cgcaatggcc ggtttccgtt gttactcaag ctttcagacg aattgattac ttcgcagcct
     9061 gtggatcagt gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat
     9121 catctaacgc gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct
     9181 ggcaagtacc acgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg
     9241 caacctgcgc ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt
     9301 ctttagtacg atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca
     9361 gaattcctgc catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag
     9421 cctggaatac attgggcgct ttttcaagcc cgcgaacgaa acggctccgc tttcagagga
     9481 ttcctgtatg acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtggcaac
     9541 gggttagctt taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca
     9601 acaaataata aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg
     9661 tagtatcgat tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct
     9721 cgggatcagc ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa
     9781 tgagctgcgc ctggaaaccg gcaacgaatc actgcaattt attgccgtac gcctgcccta
     9841 tggtgttcag gccgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg
     9901 cgtattaacc gttaatatca agggcgcggt attggccagc gaacaggcat tgcgggaagc
     9961 agacattgaa ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc
    10021 acaatatagc attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga
    10081 agccattacc ggattcttca ctaaatatgg tgacggcggt acagacatta atccgctgta
    10141 tcgtctcaac aaacgtcagg gtaaacagtt actggcggca ttaggttgcc cggaacacct
    10201 ttataagaaa gcgccaacgg ccgatctgga agatgatcgc ccttctctgc cagatgaagt
    10261 ggcactcggc gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctga
    10321 acaggtcgcc agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc
    10381 aattaccgtt ttcgatgatt tctggaaaaa gtaataattt gcacataatg gtttgtgcga
    10441 aaaagagtaa tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta
    10501 tgcaaattca atatattgca ggagtcgaat aggcctgaca ggcgtagcac gtcagacggt
    10561 gtaacctttg tcatcgaccc gcctcttttt taatcgctcc ccgcctgtta cactggatag
    10621 ataaccagca ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg
    10681 aagctgcggc aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca
    10741 cccgaccagg ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca
    10801 aaagcgttaa tatccgcagt cgggtgcttt ctcatttacg caccccggat gaagccgcca
    10861 tgctacggca atcccggcga attagctgga tctgtaccgc aggcgaaatc ggcgctctgc
    10921 tccttgaagc gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca
    10981 atcgccagct ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag
    11041 aggtggattt ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt
    11101 tgcaagcatt gcagaccatc gctgatgaac aaaaactttg ttatggcctg ctgggactgg
    11161 aaccgttaag tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat
    11221 gctgcggtaa agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt
    11281 tgcgggtggt gtgttggcct tggcaaggtg cggtggcact gaaagaacag cacccggaaa
    11341 tgactcaata tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag
    11401 aagcgacaac gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt
    11461 gtaagccgct gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc
    11521 gagccagttg atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg
    11581 cctgccatcg aaccagcacg cgccctggtc ggtagtgagc aatttatcgc ccagttgcca
    11641 cgccccgtta atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc
    11701 ggcaattctg actttcgcct tacagacatc caggcgtgtc atgatgttga aatccatcga
    11761 catctgccct tccgtcagtt tcgcttttac cacctggtcc gctgcaaagg caaaaggctg
    11821 taacggtttt aaggtatggt taaagcggtc tgcgctttca aggagcatct cgccgccttc
    11881 cagcaacgtc actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat
    11941 ggatgcaatg ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc
    12001 gcgcgtttca ccggcagcat ttcgccacag attcaccgac attttacgca tatcaaagta
    12061 ttccatactc gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt
    12121 tatcggcaac tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag
    12181 aagagaaaag aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc
    12241 ggcggtctta agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg
//
LOCUS       DAZOZY010000117        12243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZOZY010000117 DAZOZY010000000
VERSION     DAZOZY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.117"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            180..896
                     /locus_tag="ACHURQ_002795"
     CDS             180..896
                     /locus_tag="ACHURQ_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0824528.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            926..1426
                     /locus_tag="ACHURQ_002796"
     CDS             926..1426
                     /locus_tag="ACHURQ_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR0824529.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1518..4202
                     /locus_tag="ACHURQ_002797"
     CDS             1518..4202
                     /locus_tag="ACHURQ_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR0824530.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4199..5287
                     /locus_tag="ACHURQ_002798"
     CDS             4199..5287
                     /locus_tag="ACHURQ_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR0824531.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5370..6667)
                     /gene="exuT"
                     /locus_tag="ACHURQ_002799"
                     /pseudo
     CDS             complement(5370..6667)
                     /gene="exuT"
                     /locus_tag="ACHURQ_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            7150..8562
                     /gene="uxaC"
                     /locus_tag="ACHURQ_002800"
     CDS             7150..8562
                     /gene="uxaC"
                     /locus_tag="ACHURQ_002800"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR0824532.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8577..10064
                     /gene="uxaA"
                     /locus_tag="ACHURQ_002801"
     CDS             8577..10064
                     /gene="uxaA"
                     /locus_tag="ACHURQ_002801"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR0824533.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10147..10698
                     /locus_tag="ACHURQ_002802"
     CDS             10147..10698
                     /locus_tag="ACHURQ_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR0824534.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10703..11947)
                     /gene="sstT"
                     /locus_tag="ACHURQ_002803"
     CDS             complement(10703..11947)
                     /gene="sstT"
                     /locus_tag="ACHURQ_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR0824535.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3321 a         2895 c         3091 g         2936 t
ORIGIN      
        1 tatttataaa ttcctaaaaa ataagtatcg tcattgtgaa agctactaaa tttcagtaag
       61 ttaattccca gcctttaaat ttaatattaa ctaaaaatgc ttttttatat tgacttacaa
      121 taaatctaca tctctaatac accaactttt tggaaacaat attgtaagat caaatttata
      181 tggtggcgaa aattaatctc ttatcacggc ttacgccgct tttattcgtg ttcgctcctt
      241 ttttggcaca gtcaaatatg actgtttatc ctatggctgt ttccattaat agtcagggtg
      301 aaggcaacgt tcgcgtaata tctaaaagta atgaagttca gtacatcaag gcgacagtat
      361 tccgtatcga taatccatcg acgcctcagg aaaatgaagt tgagattaaa tccggtgatg
      421 caaatcatct ggttgttatg ccacctaaat tcgctttacc agccggtagt agcaaaactg
      481 tgcgctttgt tgcaatggaa ccagagcaaa aagaaaaaaa ttatcgcgtt aaatttgaag
      541 cggttcccag tattgatgac gttgccacag ataaaaaaga tctctctatg cagttaacag
      601 ttaacttaat ttgggggatt gttgttagtg ttccacctca gcaacctatt gctaagttag
      661 aagtaaatgc tgcccaaaaa ttagttaatg cagggaatca acgcttaaag attttaacga
      721 tcgcttattg taaaaataat agcaaagaaa attgtaagat acagaccgta aataaaaaca
      781 tcttccttgg tcaggaaaga aatcttgaaa gcatatctgg ctacgacaaa atcgttgtca
      841 aatataataa ctggatcacc aaagataatg gcgagtttga actggcggtc cattaattat
      901 cattgtaata aacgaaggaa tagtaatgaa aaaggtattt gcaaaatctc ttctggtcgc
      961 agcgatgttt tctgttgcag gctccgcatt ggcagtacaa aaggatatta ctgtaacggc
     1021 caacgttgat gccgctctgg atatgacaca gaccgataac accgcgttgc cgaaagcagt
     1081 agaaatgcaa tatctgccgg ggcagggtct gcaatcttac cagttgatga ccaaaatctg
     1141 gtctaacgac gttaccaaag atgtaaaaat gcagttagtt tccccggcgc aactggttca
     1201 aagcctggat gctagcaaga ttgttccgct gacagtaacc tggggcggtg aagaaattaa
     1261 agctgatgcg gcaaccactt ttaccgcaac caaaatcttt gcttccgatg cgttaactaa
     1321 cggttctctg gctaaaaacc tgatgtttgc tcaaaccacc aaaggtgttc tggagacagg
     1381 catctaccgt ggtgtagtaa gcatttatct gtctcaggat atctaatcca gataacagaa
     1441 ttaacacgta acaaagggag gggtagccct cccttatttt aactgtttta gtatttgtta
     1501 tggatttcag gattgccatg gataaaaaat tactggctct tttgatcctg gcgagtctca
     1561 gcccggcaga ggcgacatta accgaaattc ccgcagggtt tgaggttatt gctcagggac
     1621 agcaggagta tatcgaggtt tatttttcag ggaaaagtct cggtaaatat tatgcaatgg
     1681 ttaatcttga taccgtaaca tttcttgatc cagcaagtct atataacaag ctggaactgg
     1741 atgtagacga tcagaaaatc gcgcatatag tgaaagaaaa attatcgcag ccgctagctc
     1801 gccacggtga attggcttgc ggttatgtac gtactgactc agggtgtggt tttctgaata
     1861 ccgatacgct ggaaataatc tataatgatg aagaaagttc ggcaacgttg tttattaatc
     1921 cgcaatggaa ttcagctttc gatgcgaagt cattatattt aaatccagac aaaaatactg
     1981 ttaatgcttt tatacatcag caagacatca atgttctggc acaggatgat taccaatcgt
     2041 tgtctattca gggaaacggt gcgctgggaa taacagaaaa tagttatatt ggtgcacact
     2101 ggaatttcaa cggttatgat gcagatgatg tcagtgacag taatgctgac gtcagcgatc
     2161 tctattatcg ttatgatttt ttacgtcgtt attatgtgca ggcgggtcgc atggacaacc
     2221 gcacactatt taatgcacaa ggcgggaact ttacctttaa ctttctgcca ctcggtgcaa
     2281 ttgatgggat gcgtatcggg tcgacactga gctatttaaa ccaggcgcaa agccagcaag
     2341 gaaccccagt aatggttctg ctttcgcgca attctcgtgt tgacgcttat cgtaatgagc
     2401 aacttttggg atcgttttat ctcaatagtg gttcgcaatt tattgatacc agttcctttc
     2461 cgccaggcag ctacagcgtg gcattaaaag tctacgaaaa taaccaactc acccgcaccg
     2521 agcttgtgcc gtttaccaaa accggcggtc tgactgacgg aaatgcgcaa tggttcttac
     2581 aggcaggtaa aactacatca caggtttctg atgatgaaag ttcagcttat cagctaggag
     2641 tacgcctgcc attacatccg caatatgagc tctacgcagg gctggcgaat gccgatgatg
     2701 tgagtgcttt cgagttaggt aataactgga cggcagattt aggcggggtg gggaatcttg
     2761 caatcagcgc cagcgtgttc cgtaacgatg acggcggcaa aggtgatatg caacaggcca
     2821 actggagtaa tccgggatgg ccgacgttgg gcttttatcg gaccaactct gacggcgatg
     2881 cttgtacaac cgacagcaga gagagctata acgccttaag ctgttatgaa agtatttccg
     2941 cgacggtttc acagaatttt gtcggctgga atatgatgct gggttatacc cgcacacaaa
     3001 ataacactga tgatagtttg cgttgggata aacagcagag ctttgaaaat aactatcttc
     3061 gccagacaac agcgcaaagt atctctgaaa ctgtacaact tagcgcttcc cgcgcttttg
     3121 tgatgcgtga ctggattttg agtacgtcag tcggtgtttt ccatcgtaat gacaacggtg
     3181 gcgataacga cgacaacggc ttgtacttat cgttttcgtt atctgacacg ccaacgatgg
     3241 atagcaataa caacagccat tcaacaaatg tgtctacgga ttatcgttat agcgaacagg
     3301 atggcgatca aacgtcatgg cagttatcgc atacctttta taacgattca ttcagccata
     3361 aagaacttgg cgtaaccgtc ggaggcctga acaccgatac cataaacagc gcggttaacg
     3421 ggcgttggga tgggcaatac ggaaatgtct acgctaccgt atctgatagt tatgaccgta
     3481 agaatcatga tcatctttcg gcctttacgg gcacttatag ctctacgctg gctgtcagtt
     3541 gctatggcgt taatttggga gccagtggta cagacgattt gctgggtgcg gtactggtgg
     3601 atgtgaaagg cttctctgaa caggatgaag agagtcagga tctgcaactc gaagcgcggg
     3661 tggcaggcag ccgaactttg cagcttggtc aaagtgacag tgtgttgttc ccttatcctg
     3721 gatttcagtc tggttttgtt gaggttaacg acagtagcca gggcaatcag caggggacaa
     3781 caaacatcat taacggtgca gggaatcgtg aattaatgtt gttgcctggc aagctgcgct
     3841 atcgcgaagt gtctgccagc tttaactaca actatatcgg tcgtttatta ctgccggcag
     3901 cggtgaaaaa attcccgatt gtggggctga acagcgccat gttactggta gctgaagatg
     3961 gaggatttac acttgaaatt aacggcagcg aaaaagagct ctatctgctt tccgggcagc
     4021 aattccttaa gtgtccactg agtgttgtaa agaaacgcgc cagcattcgt tacagcggag
     4081 atgttacttg tagtgtggtg acttattcac aattaccgga gtcgattcag gttcaggcac
     4141 agttgaaaca gcctaaatta cgtggaaacg ttcagacggc gcaaagggag gttgcaccat
     4201 gagaaatcga ttgattgcgg cgatattggg cttgtttggc acgctcattg gcgttcaggc
     4261 agctcctgac gtgaccagtg aaattacgta tgatttggca tctggtagag cggattatta
     4321 cttctggaag gatgaagcgt cggcaggaaa taatggatat atgtggtatg aatgttcgta
     4381 tcctgacctc caacaaacct gtacagctaa tggaaatata tcgacagtac aaatctattt
     4441 aactgaacaa cgcagtggga tgcgttggcc ggtaaaactc aaaggattta aaacagccat
     4501 tgtaagtagc gatgaagcgc cgccaggatg caaggggggc aaagggcttc agacgaatct
     4561 taaggattct aatagatctt catgtacaga agatggtcaa cattattata tatacgatac
     4621 aaagtttctt acgctctacc ttgagcagac agagatgaag aatttgccga ttggtggcgt
     4681 ctggaagggg aaagttaaat tacattcgaa cagcccggcc caggactatt tcgcaaatat
     4741 taccctgaat acgctcgacc ccaaccatat tgacgtgttc ttcccggagt tcgcccacgc
     4801 cacgccaagg gtgcagttag acttgcatcc aacaggaagc gttaacggca gcaactacgc
     4861 gcaagatctg accatgttgg acatgtgcct gtacgatggt tttaacggta atgccatcag
     4921 ttatgaaatc atgctcaaag atgaagggcg accagctgca gggcgcagag acggtgactt
     4981 ctctatctat cgtcaaggag ggaccaccac cgacgaggga gaacgcattg attaccgggt
     5041 caaaatgtac aacccggaaa ccggtgggca aattgatgtg cgcaataatg aaaatatggt
     5101 ctggaacagc attaacctga aacgtgtgcg tccggtggta ctgcccggta ttcgctatgc
     5161 cgtgatgtgt gtgccgacac ccttgactct ggcagtcgat aaattcagcg tgatggataa
     5221 acaggccggg tactacatgg gcaaattgtc agtaatcttt acgccttcct tgccaaccat
     5281 caattgaccc cgcagaccag aaaaaactaa acaacgaagc cgcctgataa ggcggcttcg
     5341 tcattacaac ttacgttaaa cgttaaacgt taatgttgcg gtgcgggatc gttatgtgtt
     5401 tcctgcgcca cctcaatcgc cggtttgttc tgcaatacgg tccagataac cagcgcaccc
     5461 agcaggtcaa atactgccag aactgcgaac agcgggctga agccgatggt gtcagccagt
     5521 gcaccgacaa ccagcgcaaa cagcgtactt gccagccatg cggacatccc ggttaaaccg
     5581 ttcgccgtcg ccacttcgtt acgaccgaac acgtcagaag agagcgtaat cagcgcgcca
     5641 gacagtgcct ggtgggcaaa accaccgata cacagcagca taattgcgac atacgggttg
     5701 gtgaacagac cgatcatacc cgggccaatc atcagcacag cacccagcgt tacgaccatc
     5761 ttacgggaaa cgatcaggtt cacaccaaac caacgctgga acagcggcgg caggtaacca
     5821 ccgaggatac aaccgaggtc agcaaacagc atcggcatcc aggcgaacat cgcgatctct
     5881 ttcaggttaa agccgtaaac tttaaacatg aacagcggga tccacgcgtt aaaagtaccc
     5941 caggccggtt ctgccaggaa acgcggcagt gcgatacccc agaactgacg gttacgcagg
     6001 atctgaccaa cggacatttt cttcgccgtg ctcacctggt gctgggcttc ctgaccatta
     6061 ataatatagt cgcgttcttc atcggtcaga tgcttctggt cgcgcggatg tttatagaaa
     6121 atcagccatg ccatcgccca gataaagctc aatgcaccgg agatgataaa tgccatctgc
     6181 cagctgtgca tcacgattgc cataccacca gcggcggcgc aatcatcgca ccaatcgaag
     6241 aacctacgtt aaagtagcct actgcgatgg aacgctcttt cgccgggaac cattcggagc
     6301 tggctttcag acccgccggg atcatcgctg cttccgcggc accgaccgca ccacgagcaa
     6361 cagccaggcc accccagcta cctgccagcg cggttacacc acagaacacg gcccacagca
     6421 cagcaaacat tgcataaccg attttcgtac ccagcacatc cagtacataa cctgctaccg
     6481 gttgcatgac cgtataagca gcagaatagg ctgcgatgat ataggaatac tgttgggtgg
     6541 agatgtttaa ctcttccatc agagttggcg cagctgccgc cacagtgtta cgcgtcaggt
     6601 aaccaagcac ggtgccgagc gtcaccagtg cgatcatata ccaacgtaac cctttaattt
     6661 tacgcatgta aaacctcatc gttttgttat atccgcagga agcgcggcca tcatcccgcc
     6721 agaggctgaa gatgttagtt acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg
     6781 ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat tcatctaaca
     6841 aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc agaaagataa
     6901 cttgtcatac aactttaaaa ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca
     6961 ttacctgacg acagcgaggc cagtactggc gcggcctgca gcgagattta tcactttgag
     7021 agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg gactctggaa
     7081 attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact cgttgagagg
     7141 aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga atttgcccgc
     7201 cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg ccatttgccg
     7261 ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg gctgaaaggc
     7321 gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ccgagcgtct gtgtaccggt
     7381 gatgcatctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca cactatcggc
     7441 aacccgttat accactggac gcaccttgaa ctgcgtcgtc cgtttggtat cactggcaaa
     7501 ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact gctggcgcag
     7561 gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt cggcaccacc
     7621 gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg ctctttcacc
     7681 atcaaagtgc tgccgagctg gcgtccggac aaagccttca acatcgaaca ggcgaccttt
     7741 aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg ctttgctgac
     7801 ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg taaagtgtct
     7861 gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact ggacagcatt
     7921 ctggcgcgcc gtctggctgg cgaaaccctg agcgagcacg aagtggcaca gttcaaaact
     7981 gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac
     8041 attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc ggatgtaggc
     8101 tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct gagcaagcag
     8161 aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg cgataacgaa
     8221 gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa aatgcagttc
     8281 ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat gacccaactg
     8341 gcgcagctcg gtctgctgag tcgctttgtc ggtatgctga ctgacagccg tagcttcctg
     8401 tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg tcgctgggtg
     8461 gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa aaatatttgc
     8521 tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg gttgatatgc
     8581 aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat ttggctgaag
     8641 gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt gctcgtggac
     8701 ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat ggcctgccga
     8761 ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac aatacgcgca
     8821 cgaatctgag cgatctggat cagtatcgct atcaacctga ttttcaggat ctgcctgcgc
     8881 aagcggcaga tcgtgaagtg cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta
     8941 acgagctgtg gatcctgcca accgtgggct gtgtcaacgg catcgcgcgg caaatccaga
     9001 accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc ctcttcagcc
     9061 acacctacgg ctgctcacaa ctgggcgacg atcacatcaa cacccgcacc atgctgcaaa
     9121 acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc tgtgaaaaca
     9181 accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc gttcatttca
     9241 tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg catcagctgt
     9301 ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg aagtttggtc
     9361 tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg ctggggcgtt
     9421 tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg ccggagatgt
     9481 ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt gaaaaactgg
     9541 tcaccatggt caacgacttt aaacagtact ttattgccca tgatcagccg atctacgaaa
     9601 acccatcacc ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa tcacttggct
     9661 gtacccagaa agcgggttcc agtgtcgtgg ttgacgtgct gcgttacggc gagcgtctga
     9721 aaacgcctgg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc
     9781 tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgcggcacg ccatacggtg
     9841 gatttgtgcc gacggtgaaa atcgccacca acagtgaact ggcggcgaag aaaaaacact
     9901 ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag ttgctggaag
     9961 aatttatcga caccatcgtt gagtttgcca acggtaagca aacttgcaac gagcgtaacg
    10021 acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt
    10081 taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac ctgataagga
    10141 tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt tctgattggt
    10201 atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc
    10261 gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc cagtgccatc
    10321 cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt aatggcgatt
    10381 tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt cgaactattg
    10441 ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg
    10501 cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg ggcggggtcg
    10561 ataggcggca caatgattga gggtagtttt ctgcttatga atggcttgaa tatcattcgt
    10621 ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac ccctcccgcc
    10681 gtagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga cgatcgtctt
    10741 ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc gcggtttcgc
    10801 aagagtcctg caatacgccg atgataaagc cgacggcaac cacctgcatg gcgatatcgt
    10861 tcgaaatacc gaacatatta caggccagcg ggatcagcag cagagaaccc cccgccacgc
    10921 cggatgcgcc acaggcacac agagaagcca ccacgctcaa tagcagcgcc gtgggcagat
    10981 cgaccgggat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg
    11041 cgcccgccat attgatggtg gcacccagcg gaatagaaac ggaataggta tcgcgatcca
    11101 gattcagctt ttcacacagt gccatattca ccggaatgtt cgccgcagag ctgcgggtga
    11161 agaaggcata cacgccgctt tcgcgcaggc acagcagcac cagcgggaac gggttacgac
    11221 gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac atacagccaa
    11281 ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg
    11341 tagaagaaac cagaccaaaa ataccaatcg gtgcgaagcg aatgaccagt ttcaccataa
    11401 aggtgacggc attcgacata tcgttgacca ggtttttggt ggtctcgtta ccgtgacgca
    11461 gtgcgaaacc gaggccaatt gcccacacca ggatcccgat gtagttacct ttcagcagcg
    11521 cgtcgatggg gttggaaacc atgctcatta ccagcccgcg catcacttct acaatgcctg
    11581 acggtggcga aatatcaccc gcgctactgg ataagtgcag ggtagaaggg aaggcgaagc
    11641 tgaaaacgac tgcggccaga gcagcagaga aggtacccag cagatagagg aacaaaatag
    11701 ggcggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatc accagcatca
    11761 acaccaggat gggggcaacg gctttcagtg cgccgacgaa caaagtacct aacagaccaa
    11821 cagcttccgc cgcgggtttt gatatccatg ccagaagaat ccccagaaca aggccgacca
    11881 ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt gaacgttgcg
    11941 tagtcatttt tcgatccttt cattgtgtta tcgcacatcc ctggtgcgtt ctgtcgacgc
    12001 atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt acagcttcgg
    12061 gaagcgaaaa atgctggatt ttaaagagaa gtcatatttt gaaacttaat ggttacattt
    12121 gtttgacata aacagaggga aggactggct actaaggctg attagataac atatgggcaa
    12181 aactatttgg aatatgagaa cggttgcgct cctttaatgt gctatgccat ataaagtcac
    12241 tgg
//
LOCUS       DAZOZY010000118        12149 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZOZY010000118 DAZOZY010000000
VERSION     DAZOZY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12149
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.118"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHURQ_002804"
     CDS             complement(<1..101)
                     /locus_tag="ACHURQ_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824536.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHURQ_002805"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHURQ_002805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR0824537.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHURQ_002806"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHURQ_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR0824538.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHURQ_002807"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHURQ_002807"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR0824539.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHURQ_002808"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHURQ_002808"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR0824540.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHURQ_002809"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHURQ_002809"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR0824541.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHURQ_002810"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHURQ_002810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR0824542.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHURQ_002811"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHURQ_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR0824543.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHURQ_002812"
     CDS             9871..10449
                     /locus_tag="ACHURQ_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0824544.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHURQ_002813"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHURQ_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR0824545.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         2685 a         3304 c         2889 g         3271 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttccccccc
//
LOCUS       DAZOZY010000119        11969 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZOZY010000119 DAZOZY010000000
VERSION     DAZOZY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11969
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.119"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            126..1235
                     /gene="livK"
                     /locus_tag="ACHURQ_002814"
     CDS             126..1235
                     /gene="livK"
                     /locus_tag="ACHURQ_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR0824546.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1283..2209
                     /gene="livH"
                     /locus_tag="ACHURQ_002815"
     CDS             1283..2209
                     /gene="livH"
                     /locus_tag="ACHURQ_002815"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR0824547.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2206..3483
                     /gene="livM"
                     /locus_tag="ACHURQ_002816"
     CDS             2206..3483
                     /gene="livM"
                     /locus_tag="ACHURQ_002816"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR0824548.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3480..4247
                     /gene="livG"
                     /locus_tag="ACHURQ_002817"
     CDS             3480..4247
                     /gene="livG"
                     /locus_tag="ACHURQ_002817"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR0824549.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4249..4962
                     /gene="livF"
                     /locus_tag="ACHURQ_002818"
     CDS             4249..4962
                     /gene="livF"
                     /locus_tag="ACHURQ_002818"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR0824550.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5246..6310
                     /gene="ugpB"
                     /locus_tag="ACHURQ_002819"
                     /pseudo
     CDS             5246..6310
                     /gene="ugpB"
                     /locus_tag="ACHURQ_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            6408..7295
                     /gene="ugpA"
                     /locus_tag="ACHURQ_002820"
     CDS             6408..7295
                     /gene="ugpA"
                     /locus_tag="ACHURQ_002820"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR0824551.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7292..8137
                     /gene="ugpE"
                     /locus_tag="ACHURQ_002821"
     CDS             7292..8137
                     /gene="ugpE"
                     /locus_tag="ACHURQ_002821"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR0824552.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8139..9209
                     /gene="ugpC"
                     /locus_tag="ACHURQ_002822"
     CDS             8139..9209
                     /gene="ugpC"
                     /locus_tag="ACHURQ_002822"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR0824553.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9206..9949
                     /gene="ugpQ"
                     /locus_tag="ACHURQ_002823"
     CDS             9206..9949
                     /gene="ugpQ"
                     /locus_tag="ACHURQ_002823"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR0824554.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(9936..10376)
                     /locus_tag="ACHURQ_002824"
     CDS             complement(9936..10376)
                     /locus_tag="ACHURQ_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR0824555.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10496..>11969
                     /gene="ggt"
                     /locus_tag="ACHURQ_002825"
     CDS             10496..>11969
                     /gene="ggt"
                     /locus_tag="ACHURQ_002825"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR0824556.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         2597 a         3081 c         3460 g         2831 t
ORIGIN      
        1 cacaaaatac tgcgctaaac ccttaatcag acaggcaaaa acagtgcagt ataaaaaaag
       61 aacagtctga tttgttaaca cataaaaaca aagcaacaca acatcacgaa tggggatttt
      121 tgactatgaa acggaatgcg aaaactatca tcgcagggat gattgcactg gcaatttcac
      181 acaccgctat ggctgacgat attaaagtcg ccgttgtcgg cgcgatgtcc ggcccgattg
      241 cccagtgggg cgatatggaa tttaacggcg cgcgtcaggc gattaaagac attaatgcca
      301 aagggggaat taagggcgac aagctggttg gcgtggaata tgacgacgcc tgcgacccga
      361 aacaagccgt tgcggtcgcc aacaaaatcg ttaatgacgg cattaaatac gttattggtc
      421 atctgtgttc ttcttctacc caacctgcat cagatatcta cgaagacgaa ggtattctga
      481 tgatctcgcc gggagcgacc aacccggagc tgacccaacg cggttatcaa cacattatgc
      541 gtactgccgg gctggactct tcccaggggc caacggcggc aaaatacatt cttgagacgg
      601 tgaagcccca gcgcatcgcc atcattcacg acaaacaaca gtatggcgaa gggctggcgc
      661 gttcggtgca ggacgggctg aaagcggcta acgccaacgt cgtcttcttc gacggtatta
      721 ccgccgggga gaaagatttc tccgcgctga tcgcccgcct gaaaaaagaa aacatcgact
      781 tcgtttacta cggcggttac tacccggaaa tggggcagat gctgcgccag gcccgttccg
      841 ttggcctgaa aactcagttt atggggccgg aaggtgtggg taacgcatca ttgtcgaata
      901 ttgctggcga tgctgccgaa ggcatgttgg tcactatgcc aaaacgctat gaccaggatc
      961 cggcaaacca gggcatcgtt gatgcgctga aagcagacaa gaaagatccg tccgggcctt
     1021 atgtctggat cacctacgcg gcggtgcaat ctctggcgac tgcccttgag cgtactggca
     1081 gcgatgagcc gctggcgctg gtgaaagatt taaaagctaa cggtgcaaac accgtgattg
     1141 ggccgctgaa ctgggatgaa aaaggcgatc ttaagggatt tgattttggt gtcttccagt
     1201 ggcacgccga tggttcatcc acggcagcca agtgatcatc ccaccgcccg tgaaatgcgg
     1261 gcgggtttag aaaggttacc ttatgtctga gcagttttta tatttcttgc agcagatgtt
     1321 taacggcgtc acgctgggca gtacctacgc gctgatagcc atcggctaca ccatggttta
     1381 cggcattatc ggcatgatca acttcgccca cggcgaggtt tacatgattg gtagctatgt
     1441 ctcctttatg atcatcgccg cactgatgat gatgggcatt gataccggct ggctgctggt
     1501 ggcggcggga ttcgtcggcg caatcgtcat tgccagcgcc tacggctgga gtatcgaacg
     1561 ggtagcctat cgcccggtgc gtaactctaa gcgcctgatt gcgctcatct cggcaatcgg
     1621 catgtccatc ttcctgcaaa actacgttag cctgaccgaa ggttcgcgtg acgtggcgct
     1681 gcccagtctg tttaacggtc agtgggtggt ggggcatagc gaaaacttct ctgcctctat
     1741 taccaccatg caggcagtga tctggattgt tactttcctc gccatgctgg cgctgacgat
     1801 tttcattcgt tattcccgta tggggcgcgc ctgccgtgcc tgcgcggaag atctgaaaat
     1861 ggcgagcctg cttggcatca acaccgaccg ggtgattgct ttgacctttg ttattggcgc
     1921 ggcgatggcg gcagtggctg gcgtactgct cggtcagttc tacggcgtca ttaaccccta
     1981 tatcggcttt atggccggga tgaaagcctt taccgctgcg gtgctcggtg ggattggcag
     2041 cattccaggg gcgatgattg gcgggctgat cctggggatt gcggaggcgc tctcttctgc
     2101 ctatctgagt acggaatata aagatgtggt ctcattcgcc ctgctgattc tggtgctgct
     2161 ggtgatgccg accggtattc tgggtcgccc ggaggtagag aaagtatgaa accgatgcat
     2221 attgcaatgg cactgctctc tgccgcgatg ttctttgtcc tcgctggcgt ctttatgggc
     2281 gtgcaactgg agctggatgg caccaaactg gtggtcgaca gcgcttcgga tgtccgttgg
     2341 cagtgggtgt ttatcggcac ggcggtggtc tttttcttcc agcttttgcg acctgctttc
     2401 cagaaagggc taaaaagcgt ttccgggccg aagtttattc tgcccgcgat tgatggctcc
     2461 acggtgaagc agaaactgtt cctcgtggcg ctgctggtgc ttgcggtgtc gtggccgttt
     2521 atggtgtcac gtgggacggt ggatatcgcc actctgacca tgatctacat tatcctcggt
     2581 ctcgggctga atgtggttgt tggtctttct ggtctgctgg tgctggggta cggcggtttt
     2641 tacgccatcg gcgcttacac ttttgcgctg ctcaatcact attacggtct ggggttctgg
     2701 acctgtttgc cgattgccgg actgatggca gcggcggcgg gcttcctgct tggttttccg
     2761 gtgttgcgtc tgcgcggtga ttatctggcg atcgttaccc tcggtttcgg cgaaattgtg
     2821 cgcatattgc tgctcaataa caccgaaatt accggcggcc cgaacggtat cagtcagatc
     2881 ccgaaaccga ctttcttcgg ccttgagttc agccgtaccg cccgtgaagg cggttgggac
     2941 acgttcagta atttctttgg cctgaaatac gatccctccg atcgcgtcat cttcctgtat
     3001 ctggtggcgt tgctgctggt ggtgctaagc ctgtttgtca ttaaccgcct gctgcggatg
     3061 ccgctggggc gtgcgtggga agcgttgcgt gaagatgaga tcgcctgccg ttcgctgggc
     3121 ttaagcccgc gtcgtatcaa gctgactgcc tttaccatca gtgccgcgtt tgccggtttt
     3181 gccggaacgc tgtttgctgc gcgccagggc tttgtcagcc cggaatcctt cacctttgct
     3241 gaatcggcgt ttgtactggc gatagtggtg ctcggcggta tgggatcgca gtttgcggtg
     3301 attctggcgg caattttgct ggtggtatcg cgcgaattga tgcgtgattt caacgaatac
     3361 agcatgttaa tgctcggtgg tttgatggtg ctgatgatga tctggcgtcc gcagggcttg
     3421 ctgcccatga cgcgcccgca actgaagctg aaaaacggcg cagcgaaagg agagcaggca
     3481 tgagtcagcc attattatct gtgaacggcc tgatgatgcg cttcggcggc ctgctggcgg
     3541 tgaacaacgt caatcttgaa ctgtacccgc aggagatcgt ctcgttaatc ggccctaacg
     3601 gtgccggaaa aactacggtt tttaactgtc tgaccggatt ctacaaaccc accggcggca
     3661 ccattttact gcgcaatcag cacctggaag gtttaccggg gcagcaaatt gcccgcatgg
     3721 gcgtggtgcg caccttccag catgtgcgtc tgttccgtga aatgacggta attgaaaacc
     3781 tgctggtggc gcagcatcaa caactgaaaa ccgggctgtt ctctggcctg ttgaaaacgc
     3841 cttctttccg tcgcgcccag agcgaagcgc tcgaccgcgc cgccacctgg cttgagcgca
     3901 ttggtttgct ggaacacgcc aaccgtcagg cgagtaacct ggcctatggt gaccaacgcc
     3961 gtctggagat tgcccgctgc atggtgacgc agccggagat tttaatgctc gacgaaccgg
     4021 cggcaggtct taacccaaaa gaaaccaaag agctggatga gctgattgcc gaactgcgca
     4081 atcatcacaa caccactatc ttgttgattg aacacgatat gaagctggtg atggggattt
     4141 cagaccgaat ttatgtggtc aatcagggga cgccactggc aaacggtacg ccggagcaaa
     4201 tccgtaataa cccggacgtg atccgtgcct atttaggtga ggcataagat ggaaaaagtc
     4261 atgttgtcct ttgacaaagt cagcgcccac tacggcaaaa tccaggcgct gcatgaggtg
     4321 agcctgcata tcaatcaggg cgagattgtc accctcattg gcgcgaacgg ggcggggaaa
     4381 accaccttgc tcggcacgtt atgcggcgat ccgcgcgcta ccagcgggcg aattgtgttt
     4441 gatgataaag acattaccga ctggcagacg gcgaaaatca tgcgcgaagc ggtagcgatt
     4501 gtcccggaag ggcgtcgcgt cttctcgcgg atgacggtgg aagagaacct ggcgatgggc
     4561 ggcttttttg ccgaacgcga ccagttccag gagcgcataa agtgggtgta tgaactgttc
     4621 ccacgtctgc atgagcgccg tgttcagcgg gcaggcacca tgtccggcgg tgagcaacaa
     4681 atgctggcga ttggtcgcgc gctgatgagc aacccgcgtt tgctgctact tgatgagcca
     4741 tcgcttggtc ttgcgccgat tatcatccag caaattttcg acaccatcga gcagctgcgc
     4801 gagcagggga tgactatctt cctcgtcgag cagaacgcca accaggcgct aaagctggcg
     4861 gatcgcggct acgtgctgga aaacggccat gtggtgctct ccgatactgg tgatgcacta
     4921 ctggcgaatg aagcggtgag aagtgcgtat ttaggcgggt aataacacgt tgattgataa
     4981 ggagtcaaaa gactcctttg agacaggtga caaatgtaaa attgcctgat gcgctgcgct
     5041 tatcaggcct actgggtgag ttgcaatata ttgaatttgc acgatcttgt aggccggata
     5101 aggcgtttac gccgcatccg gcatgaaacg atgagtaatc tgtagagttt gattcagacc
     5161 ttctattttt ccgcttatcc gtgccccatc tcccattttc cctcacccac gccgtcaccg
     5221 ccttgtcatc tttctgacac cttacttgca ggtttatgaa gttggcaccg ccaccatgat
     5281 ggcatcgaaa gccattaaac cggtatatga cgtgtttaaa gaggcaggga ttcaattcga
     5341 tgagtcgcag tttgtgccga cggtttccgg ttactactcc gacagcaaaa cgggccactt
     5401 actctcccag ccattcaaca gctcgactcc cgttctctat tacaacaaag acgccttcaa
     5461 gaaagcgggg ttagacccgg aacagccgcc gaaaacctgg caggatctgg cggactcttc
     5521 cgcaaaactg aaagcctccg gtatgaagtg cggctacgcc agcggctggc agggctggat
     5581 ccaactggaa aactttagcg cctggaacgg tctgccgttt gccagcaaaa acaacggctt
     5641 tgacggcacg gacgcggtgc tggagttcaa caagccggag caggtgaaac acattgccat
     5701 gctcgaagag atgaacaaga agggcgactt cagctacgtc ggtcgtaagg atgaatccac
     5761 cgagaagttc tataacggtg attgcgcgat gaccaccgcc tcttccggtt ctctcgctaa
     5821 tattcgtgag tacgccaaat ttaactatgg cgtaggcatg atgccttacg acgccgatgc
     5881 gaaagatgca ccgcaaaacg ccattatcgg cggggccagc ctgtgggtga tgcagggcaa
     5941 aaataaagaa acgtataccg gcgtggcgaa gttcctcgac ttcctcgcga agccagaaaa
     6001 cgctgccgag tggcatcaga aaaccggtta tctgccaatc accaaagcag cgtatgacct
     6061 gacccgtgag cagggctttt atgagaaaaa cccaggggcg gataccgcga cgcgtcagat
     6121 gctgaataag ccgccgttgc cgttcaccaa agggctgcgt ctgggcaaca tgccgcagat
     6181 ccgcgtgatt gtggatgaag agctggagag cgtgtggacc ggtaagaaga caccacagca
     6241 ggcactggat accgccgttg agcgtggaaa tcagttgctg cgccgctttg agaaatcgac
     6301 gaagtcttaa tcagtgtaat gtcggatgcg tttcgcttat ctgacctggc atcgcgtgta
     6361 ggccggataa gcgaagcgca tccggcacag ttcaggaatt aaccgtaatg tcatcatccc
     6421 gtccggtgtt ccgctcgcgc tggctgcctt atctgctggt cgcgccgcag ctcatcatca
     6481 ccgttatctt ttttatctgg cctgcgggcg aagcgttgtg gtactcgcgg caaagcgtcg
     6541 atccgtttgg tttctccagc cagtttgtcg gtctggataa cttcgtcacg ctgttccatg
     6601 acagctacta tctagactcc ttctggacga cgataaaatt cagcaccttt gtcaccgtca
     6661 gcggtttgct ggtatcgctg ttctttgcgg cgctggtgga gtacatcgtg cgcggcagcc
     6721 gtttctatca aaccttgatg ctgctgccgt atgccgtggc tcccgccgtt gccgccgtat
     6781 tgtggatctt cctgtttaac cccggtcgcg ggctgatcac ccattttctc gctgagttcg
     6841 gctacgactg gaaccacgcg caaaacagcg gtcaggcaat gtttctggtg gtgtttgcct
     6901 cggtgtggaa gcaaatcagc tacaactttc tgttcttcta tgccgcgctg cactccattc
     6961 cccgttcgtt gattgaagcc gcagctattg acggcgcagg gccgattcgc cgcttcttta
     7021 agattgctct gccgcttatc gctccggtga gtttcttcct gctggtggtg aacctggtgt
     7081 acgccttctt cgacaccttc ccggtgatcg acgccgccac gtccggcggg ccggttcagg
     7141 ccaccactac gctgatttat aagatctacc gcgaaggctt taccggactg gatctggctt
     7201 cgtctgccgc gcagtcggtg gtgttgatgt tcctcgtcat cgtgctgacg gtggtgcagt
     7261 tccgctatgt tgaaagcaag gtgcgttacc aatgattgag aaccgtccgt ggctgacgat
     7321 attcagccat accatgctga tcctcgggat cgcggtgatc ctcttcccgc tgtacgtggc
     7381 gtttgtcgcg gcgacgctgg ataaacaggc cgtctatgcc gcgccgatga cgctcatccc
     7441 cggcacacat ctgctggaaa atatccacaa catctgggtt aacggggtag gcacgaatag
     7501 cgcgccgttc tggcggatgt tgcttaacag ctttgtgatg gcgttcagca ttacgctcgg
     7561 caaaattacc gtctcgatgc tctcggcatt tgccattgtc tggtttcgtt ttccgctacg
     7621 taacctcttc ttctggatga tttttatcac cctgatgctg ccggttgaag tacgtatctt
     7681 cccgacggtg gaagtcatcg ccaacctgaa aatgctcgac agctacgccg gtttaacgct
     7741 gccgctgatg gcctcggcga ccgccacgtt tctgttccgc cagttcttta tgaccctacc
     7801 ggatgaattg gtggaagccg cgcggatcga cggcgcgtca ccaatgcgtt tcttttgcga
     7861 catcgttttt ccgttgtcca aaactaatct ggcggcgctg tttgtgatca cctttatcta
     7921 cggctggaat cagtatttgt ggccgttgtt gattattacc gacgttgatc tcggcaccac
     7981 cgtggcaggg atcaaaggga tgatcgctac aggcgaaggc accacggaat ggaactcggt
     8041 aatggcggcg atgttgttaa cgcttatccc tccggtggtg attgttttag tgatgcagcg
     8101 tgccttcgtg cgcggcctgg tcgatagtga gaaataagat ggcaggactg aaattacagg
     8161 cagtaaccaa aagctgggat ggcaaaaccc aggtcattaa accgctgacc cttgatgtgg
     8221 cggatggcga atttatcgtg atggtcgggc cgtctggctg cgggaaatcg acgctgctgc
     8281 gcatggttgc cgggctggag cgggtgacag aaggcgatat ctggatcaac gaccagcgcg
     8341 tgaccgaaat ggagccgaaa gatcgcggga ttgcgatggt gttccagaat tacgcgcttt
     8401 atccgcatat gagcgtagag gagaacatgg cgtgggggct gaaaattcgc ggcatgggca
     8461 agcagcaaat tgccgagcgc gttaaagaag cggcgcgcat tctggaactg gacggtctgc
     8521 ttaagcgtcg tccgcgtgag ctatccggcg gtcagcgcca gcgtgtggcg atgggccgcg
     8581 cgattgtgcg cgatccggcg gtgttcttgt ttgatgagcc gctctctaac ctcgatgcca
     8641 agctgcgcgt gcagatgcgt cttgaactgc aacagttgca ccgtcgcctg aaaacgactt
     8701 cactctacgt tactcacgat caggttgaag cgatgacgct cgcccagcga gtaatggtga
     8761 tgaacggtgg cgttgccgaa cagattggca caccagttga agtctacgaa aagcccgcca
     8821 gcctgtttgt ggcgagtttt atcggcagcc cggcgatgaa cctgctgaca ggccgcgtga
     8881 ataatgaagg cacgcatttc gaactggacg gcggtatcgt gctaccgcta aacggtggct
     8941 accgtcagta tgccgggcgt aaaatgactc tcggcattcg cccggaacat atagcgctaa
     9001 gctcgcaggc agaaggtggt gtgccgctgg tgatggacac gctggagatc ctcggcgcag
     9061 ataacctggc gcacggacgc tggggcgagc agaagctggt ggtgcgactg gcgcatcagg
     9121 agcgcccgac ggcaggcagc acgctgtggc tgcatttggc ggaaaatcag ctgcatcttt
     9181 ttgatggtga aacaggacaa cgagtatgag taactggcct tatccccgca tcgtcgctca
     9241 tcgtggcggc ggtaagctgg ccccggaaaa caccctggcg gcaatcgacg tcggggcaaa
     9301 atacggtcat aagatgatcg aatttgacgc gaagttatcg aaagatggcg agatcttcct
     9361 gctccatgac gacaatctcg aacgcaccag caacggctgg ggcgtcgcgg gtgaactgaa
     9421 ctggcaggat ttactgcgcg tggatgcggg cagttggtac agcaaagcgt ttaaaggtga
     9481 gccgctgccg ttgctttcgc aggtggcgga acgctgtcgc gaacacggga tgatggcgaa
     9541 tatcgaaatc aaacccacca ctggcactgg accattaacg ggcaaaatgg tggcgctggc
     9601 ggcacgccaa ctgtgggccg gtatgacgcc gccgctgctg tcatcgtttg agattgatgc
     9661 tttagaagct gcacaacagg cggcaccgga actgccgcgt ggtttattgc ttgatgagtg
     9721 gcgcgacgac tggcgcgaac tgaccgcgcg gctgggctgc gtctctattc atctcaatca
     9781 taagttactc gataaagcgc gagtgatgca gttgaaagac gccggattac ggattctggt
     9841 ttataccgtc aataaacccc agcgcgcggc agagttgctg cgctggggcg tggattgcat
     9901 ctgtaccgat gcgattgacg tgattggtcc gaactttacg gcccaatagt tttcaacgga
     9961 atgtcaggct gcggcgtgct gggctgattc agcatgtcgc cgtttctcct ttccggcagc
    10021 atatgctgct gattcaacga actatccgga ttacggttgc tgtttaacat cccgccgttg
    10081 gtgttgggca gcatttgctg ccgggcggga tttcgctccc ccggctgtga ctgcaacacc
    10141 cgctgagaat tattgtttat ctggttttct aaatgctgct gttgcaactg agtttgcgtt
    10201 ttcagttgct gattcagcat ccctttttgc tggatttgct gagtctgcat ctgggtttgc
    10261 atccgctgct ggctgggtat ctgatacccc ggctggttag ggttgttcag agtattaatg
    10321 ggctgtgcaa agccgacaaa cggcaggagt gccgtaagaa tcagaagtcg tttcatcgcg
    10381 tatcctcctc tgaagatatc ctttaagttt actcgcttcc cgacaaaacg atgattaatt
    10441 cagagttata taccaggctt agctggggtt gccccttaat ctctggagaa taacgatgat
    10501 aaaaccgacg tttttacgcc gggtggccat tgctgctctg ctctcaggaa gttgttttag
    10561 cgccgccgcc gcgcctcctg cgccgcccgt ctcgtatggt gtggaggaag atgtcttcca
    10621 cccggtacgc gcgaaacagg gaatggtagc gtctgtggac gccactgcca ctcaggtggg
    10681 ggtggatatc ctcaaggagg gcgggaatgc cgttgatgcc gccgtggcgg tgggctacgc
    10741 gctggcggta acgcatccgc aggcagggaa tctgggcggt ggtggtttta tgttaatccg
    10801 ctcgaaaaat ggcaatacca cggctatcga tttccgcgaa atggcacccg ccaaagcgac
    10861 ccgcgatatg ttcctcgatg atcagggcaa cccggacagc aaaaaatcac tcacttcgca
    10921 tctggcttcc ggcacaccgg gtacggtagc aggtttctcg ctggcgctgg ataaatacgg
    10981 caccatgccg ctgaacaaag tcgtgcagcc cgcgtttaaa ctggcacgcg atggttttat
    11041 cgttaacgac gcgctggctg acgatctcaa aacctacggt agcgaagtgt tgccgaatca
    11101 cgaaaacagt aaagctatct tctggaaaga gggcgagccg ctgaaaaagg gcgacacgct
    11161 ggtgcaggcg aacctggcaa agagcctgga gatgattgct gaaaacggcc cggacgaatt
    11221 ctataaaggc acgattgcgg aacagatcgc ccaggagatg cagaaaaacg gtggcttgat
    11281 cactaaagaa gatttagcgg cctataaagc ggtcgaacgc actccgataa gcggcgatta
    11341 tcgcgggtat caggtttact ccatgccacc gccatcctcc ggcgggatcc atatcgtaca
    11401 aatcctcaat attctggaaa acttcgatat gaagaaatac ggctttggca gcgccgatgc
    11461 gatgcaaatc atggcagaag cggagaaata cgcctacgcc gaccgctcgg aatatcttgg
    11521 cgacccggat tttgtcaaag tcccgtggca ggcactgacc aataaagcct atgccaaatc
    11581 cattgccgat caaattgata tcaacaaagc gaagccgtcc agtgagattc gccctggcaa
    11641 gcttgcgcct tatgagagta atcaaactac ccattactct gtagtggata aagacggtaa
    11701 cgcggtggcg gtgacctata cgctgaacac caccttcggt atgggcattg tcgcgggcga
    11761 gagcggtatt ctgcttaata atcagatgga tgatttctcc gccaaaccgg gcgtaccgaa
    11821 catttacggg ctggtgggcg gtgatgccaa cgccgtcggg ccgaataaac gtccgctgtc
    11881 atcgatgtca ccaaccattg tggtgaaaga cggtaaaact tggctggtta ccggtagtcc
    11941 tggcgggagt cggattatta ccaccgtgc
//
LOCUS       DAZOZY010000120        11950 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZOZY010000120 DAZOZY010000000
VERSION     DAZOZY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.120"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHURQ_002826"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHURQ_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR0824557.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1241..1345)
                     /gene="sgrT"
                     /locus_tag="ACHURQ_002827"
     CDS             complement(1241..1345)
                     /gene="sgrT"
                     /locus_tag="ACHURQ_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR0824558.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLM"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHURQ_002828"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHURQ_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR0824559.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHURQ_002829"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHURQ_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR0824560.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHURQ_002830"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHURQ_002830"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR0824561.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHURQ_002831"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHURQ_002831"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR0824562.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHURQ_002832"
     CDS             complement(6574..7338)
                     /locus_tag="ACHURQ_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR0824563.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHURQ_002833"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHURQ_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR0824564.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHURQ_002834"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHURQ_002834"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR0824565.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHURQ_002835"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHURQ_002835"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR0824566.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
BASE COUNT         2798 a         3349 c         3166 g         2637 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctacatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc
//
LOCUS       DAZOZY010000121        11912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZOZY010000121 DAZOZY010000000
VERSION     DAZOZY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.121"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHURQ_002836"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHURQ_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR0824567.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHURQ_002837"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHURQ_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR0824568.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHURQ_002838"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHURQ_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR0824569.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHURQ_002839"
     CDS             complement(3668..3898)
                     /locus_tag="ACHURQ_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR0824570.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHURQ_002840"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHURQ_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHURQ_002841"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHURQ_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR0824571.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHURQ_002842"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHURQ_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHURQ_002843"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHURQ_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR0824572.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHURQ_002844"
     CDS             7629..8642
                     /locus_tag="ACHURQ_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0824573.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKI
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHURQ_002845"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHURQ_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHURQ_002846"
     CDS             9533..10327
                     /locus_tag="ACHURQ_002846"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0824574.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHURQ_002847"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHURQ_002847"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR0824575.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2875 a         2893 c         3197 g         2947 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaatc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gt
//
LOCUS       DAZOZY010000122        11820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZOZY010000122 DAZOZY010000000
VERSION     DAZOZY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.122"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            22..1099
                     /gene="ygjJ"
                     /locus_tag="ACHURQ_002848"
                     /pseudo
     CDS             22..1099
                     /gene="ygjJ"
                     /locus_tag="ACHURQ_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1116..3467
                     /gene="ygjK"
                     /locus_tag="ACHURQ_002849"
     CDS             1116..3467
                     /gene="ygjK"
                     /locus_tag="ACHURQ_002849"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR0824576.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            3693..5711
                     /gene="fadH"
                     /locus_tag="ACHURQ_002850"
     CDS             3693..5711
                     /gene="fadH"
                     /locus_tag="ACHURQ_002850"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR0824577.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(5756..6172)
                     /gene="higA"
                     /locus_tag="ACHURQ_002851"
     CDS             complement(5756..6172)
                     /gene="higA"
                     /locus_tag="ACHURQ_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR0824578.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6169..6483)
                     /gene="higB"
                     /locus_tag="ACHURQ_002852"
     CDS             complement(6169..6483)
                     /gene="higB"
                     /locus_tag="ACHURQ_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR0824579.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(6768..7904)
                     /gene="rlmG"
                     /locus_tag="ACHURQ_002853"
     CDS             complement(6768..7904)
                     /gene="rlmG"
                     /locus_tag="ACHURQ_002853"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR0824580.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            7989..8492
                     /locus_tag="ACHURQ_002854"
     CDS             7989..8492
                     /locus_tag="ACHURQ_002854"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR0824581.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            8569..9252
                     /locus_tag="ACHURQ_002855"
     CDS             8569..9252
                     /locus_tag="ACHURQ_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR0824582.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9331..10317
                     /locus_tag="ACHURQ_002856"
     CDS             9331..10317
                     /locus_tag="ACHURQ_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR0824583.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            10600..11565
                     /gene="alx"
                     /locus_tag="ACHURQ_002857"
     CDS             10600..11565
                     /gene="alx"
                     /locus_tag="ACHURQ_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR0824584.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2801 a         3057 c         3200 g         2762 t
ORIGIN      
        1 tttagctcat ggattaacac aatgaaatta atcactgcac catgcagagc attacttgct
       61 ctgccgtttt gctacgcctt ttctgcggca ggagaagaag cacgtccggc agaacatgac
      121 gacacaaaaa cacccgcaat tacctcgaca tcttctcctt catttcgttt ttacggcgaa
      181 ttagggatta gggggtggtg gatatatgga tttagagggt gagaataaac ataaatacag
      241 cgacggtacc tatattgaag gtggcctgga gatgaagtac ggctcctggt tcggcctgat
      301 ttacggcgaa ggctggaccg tgcaggccga ccacgacggc aatgcctggg tgccagacca
      361 tagctggggt ggtttcgagg gcggaattaa ccgtttctat ggcggttatc gtaccaatga
      421 tggcaccgaa atcatgctca gtctgcgtca ggattcctcg ctggatgacc tgcaatggtg
      481 gggcgatttc acccccgatc tgggctacgt cattcccaat acccgcgaca ttatgactgc
      541 gctgaaggta cagaacttaa gcggcaactt tcgttatagc gtcaccgcga ctcctgccgg
      601 acatcatgat gaaagcaaag cctggctaca ttttggcaaa tacgatcgct atgacgacaa
      661 atacacctat ccggcaatga tgaacggtta catccagtat gaccttgccg aaggcatcac
      721 ctggatgaac ggtctggaaa tcaccgacgg cacaggacag ctctatctca cgggcctgct
      781 aactcctaac tttgccgctc gcgcctggca ccataccgga cgcgccgacg ggctggacgt
      841 accgggaagt gaaagtggga tgatggtgag cgccatgtat gaagcgttaa agggcgttta
      901 tctctccacc gcttacacct acgccaaaca tcgccctgac cacgctgacg atgaaaccac
      961 ctctttcatg cagtttggta tctggtacga atacggcggc ggacgtttcg ccacggcttt
     1021 tgatagccgc ttctacatga aaaatgcctc tcacgatccc agcgaccaaa tcttcctgat
     1081 gcaatatttc tactggtaat aaggactgta atttcatgaa aataaaaact attttaacgc
     1141 cagtaacctg cgctctgctg ataagttttt ccgcccatgc cgctaacgcc gacaattata
     1201 aaaacgtgat taaccgtact ggcgcgccgc agtacatgaa ggattacgat tacgacgatc
     1261 accagcgttt taatccgttt ttcgatctcg gagcctggca tggtcatctg ttgcctgacg
     1321 gccctaacac catgggcggc tttccgggcg ttgcgctgct gacggaagag tacatcaact
     1381 ttatggccag caatttcgac cgcctgaccg tctggcagga cggcaagaaa gtcgacttta
     1441 cgctggaggc atacagtatt cccggtgcgc tggtgcaaaa actgacagca aaagatgtgc
     1501 aagtcgaaat gactctgcgc ttcgccacgc cgcgcacgtc actactggaa accaaaatca
     1561 ccagcaataa accgctggat ctggtgtggg acggcgaact gctggaaaaa ctggaagcga
     1621 aagaagggaa accgctttcc gataaaacca ttgctggcga ataccctgac tatcagcgca
     1681 aaatcagcgc cacccgtgat ggcctgaaag tcacctttgg caaagtgcgc gccacctggg
     1741 atctgctgac ctccggcgaa tcggaatatc aggtgcataa atccctgccg gtgcagactg
     1801 aaatcaacgg taatcgcttt accagtaagg cgcatatcaa cggttcgacc acgctctaca
     1861 ccacctattc ccatctgctg accgctcagg aagttagcaa agagcaaatg cagatccgcg
     1921 atattctggc gcgtccggcg ttttatctca ccgcctcgca gcaacgctgg gaagaatacc
     1981 tgaagaaagg gttaaccaat ccggatgcga cgccggaaca gacgcgcgtc gcggtgaaag
     2041 cgatcgaaac gctcaacggt aactggctct caccgggcgg tgcggtgaaa tttaacaccg
     2101 ttacaccgtc ggtgaccggg cgctggttct ccggcaatca gacctggccg tgggatacct
     2161 ggaagcaggc gtttgcgatg gcgcatttca atccggacat cgccaaagag aatatccgcg
     2221 cggtcttctc ctggcagatc cagcctggcg atagcgtgcg tccgcaggat gtgggctttg
     2281 tccccgacct gatagcgtgg aaccttagcc ccgagcgtgg tggcgatggc ggcaactgga
     2341 acgaacgtaa taccaaaccc agccttgccg cctggtcggt gatggaagtg tacaacgtca
     2401 cccaggataa agcctggttg gcagagatgt acccgaaact ggtggcctat cacgactggt
     2461 ggttacgtaa ccgcgatcac aacggcaacg gcgtgccgga atatggcgcg acccgcgaca
     2521 aagcccacaa cactgagagc ggcgagatgc tgtttacggt gaaaaaaggc gataaagaag
     2581 agacgcagtc tgggctgaac aactacgccc gcgtggtgga gaaaggcctg tacgacagtc
     2641 tggaaattcc ggcacaggtt gctgcatcgt gggaatcggg gcgtgatgat gccgccgtct
     2701 ttgggtttat cgacaaagaa cagctggata aatatgtcgc taatggcggc aaacgtagcg
     2761 actggacggt gaaattcgcc gaaaaccgca gtcaggacgg aacgttgctg ggctactcgc
     2821 tattgcagga gtcggtggat caggccagct atatgtacag cgataaccat tatctggcgg
     2881 agatggcaac catcctcggt aagccagaag aggccaaacg ctatcgccag ttggcacagc
     2941 agctcgcgga ctacatcaac acctgtatgt tcgacccgac tacccagttc tactatgacg
     3001 tacgtattga agataaacca ctggcgaacg gctgcgcggg caaaccgatt gttgagcgcg
     3061 gtaaagggcc ggaaggctgg tcgccgctgt ttaacggtgc ggcaacgcag gccaacgccg
     3121 acgcggtggt gaaggtgatg ctcgatccta aagagttcaa cacctttgtc ccgctgggaa
     3181 cggcggcgtt aaccaatccg gcttttggcg ctgatatcta ctggcgtggg cgcgtatggg
     3241 tggatcagtt ctggtttggt ctgaaaggga tggagcgtta cggttatcgt gatgatgccc
     3301 tgaagctggc ggatacgttc ttccggcacg ccaaaggatt aaccgccgat ggcccaattc
     3361 aggagaatta caacccgctg actggcgcac agcaaggcgc accaaatttc tcctggagtg
     3421 ccgctcattt gtatatgttg tataacgatt ttttccgtaa gcagtaattt gcaacggctg
     3481 gcggatgcgg cgtgaacgcc ttatccgccc tacatgtgca atcccccagg ccggataaaa
     3541 cgcggcaagc gttgcatccg gcgttaccac cgggcgtatt ctttttgaat cccatcacaa
     3601 accccgcact ccccttttcc cttttctcca tcgacggcta aattagaact catccgacca
     3661 cataacaatt attttacata ctggacaatt ttatgagcta cccgtcgctg ttcgccccgc
     3721 tggatttagg ttttaccacg ttaaaaaacc gcgtgttgat gggctcaatg cacaccgggc
     3781 tggaggaata cccggacggt gccgagcggc tggcagcgtt ttatgccgaa cgcgcccgtc
     3841 acggcgtggc gctgattgtc agcggcggca ttgcaccaga tttaacaggc gttggcatgg
     3901 aaggtggcgc aatgctcaac gacgccagcc agatcccaca ccatcgcacc attaccgaag
     3961 cggtacatca ggaaggcggc aaaatagccc tgcaaatttt gcataccggg cgctacagct
     4021 accaaccgca tctggtcgct ccctccgcat tgcaggcccc catcaaccgt ttcgttcctc
     4081 acgaactcac ccatgaagag atcctgcaac tgatcgacga tttcgcccac tgcgcgcaac
     4141 tggcacggga ggcaggatac gacggtgtag aggtaatggg gtccgaaggg tatttgatca
     4201 acgaatttct gacgctgcgc accaatcagc gtagtgacca gtggggcggc gattaccgca
     4261 accggatgcg atttgccgta gaagtagtgc gtgcggtgcg cgaacgcgtc ggcaacgact
     4321 tcattattat ctaccgactg tcgatgctcg acctggtcga agacggcggg acttttgccg
     4381 aaacggtaga gctggcgcag gccattgaag cggcgggcgc gaccattatc aacaccggca
     4441 ttggctggca tgaagcacgt attccgacca ttgccacgcc cgtgccgcgc ggcgcattta
     4501 gctgggtcac gcgcaaactg aaaggccacg tctcgctgcc gctggtaacc accaaccgga
     4561 ttaacgatcc gcaggttgcc gacgatattc tctcgcgcgg cgatgccgat atggtatcga
     4621 tggcacgacc gtttcttgct gatgcggaac tgctgtcaaa agcgcaatcg gggcgagccg
     4681 atgagatcaa cacctgcatt ggctgcaatc aggcctgcct cgatcagatc ttcgttggca
     4741 aagtcacctc gtgcctggtg aatcctcgcg cctgccacga aaccaaaatg ccaatccttc
     4801 ctgccgtgca gaaaaaaaat ctggcggtgg tcggtgcggg acctgctggg ctggcgtttg
     4861 ccattaacgc ggcggcgcgt gggcatcagg taacattgtt tgatgcccac agcgagatcg
     4921 gcgggcagtt taatatcgcc aaacagatcc ccggcaaaga ggagttttac gaaacgctgc
     4981 gctattacca ccggatgatc gaagtgacgg gcgtgacgct gaaactcaat cacaccgtga
     5041 cggcggatca gttacaggcg ttcgatgaaa cgatcctcgc cagtgggatc gtgccgcgcg
     5101 ttccgcctat cgacgggatc gatcatccga aggtattgag ttatctcaat gtactgcgcg
     5161 acaaagcgcc ggtaggcaac aaggttgcca tcatcggttg tggcgggatt ggttttgata
     5221 cggcgatgta tttaagtcag ccgggcgaat ccaccagcca gaatatcgcc aggttctgta
     5281 atgagtgggg gatcgacagc agcctacaac aggctggtgg cttaagcccg cagggaatgc
     5341 agatcccgcg tagcccacgg caaattgtga tgctccagcg caaagccagc aaaccgggac
     5401 aggggttagg caaaaccacc ggctggatcc atcgcaccac cctgctctcg cggggtgtga
     5461 aaatgatccc aggcgtaagt tatcagaaga ttgacgatga cgggctgcat gtggtgatca
     5521 acggcgaaac gcaggtatta gcagtggaca atgtggtgat ctgcgcaggg caagagccaa
     5581 accgcgcgct ggcgcaaccg ctgattgata gcgggaaaac tgtgcattta attggcggct
     5641 gcgatgtggc tatggagctg gacgcacgac gggcaattgc ccagggaaca cggctggcgc
     5701 tggagattta aatcgttatt ttaagcgccg gatgcaacgc atccggcacg tcgcgttaat
     5761 caataaacaa ggcgggagaa atgccgaatc gcgttgccaa ttttttagcg tgttccagcg
     5821 ttaatttcct cttcccgctc aaaacgcgtg acaccataga tttactgcca atttccggca
     5881 gatcggcaag ggttaaacca tattgatcca taagggtacg aatcacggct ataccgccag
     5941 gcatggcttg agccatggca ttaaattccg caaattcggg cgctgattct tcccacgcgg
     6001 ttattttggc acacaccaga tccagcaagg ggttttcagg atcgttgagc agcagatgat
     6061 ctaccagttc cagcgcctgg gtgtattgtt cctcgttctg aatacccgcc agaaaaggtg
     6121 ccaccgcagt tagcttttct cctgcttgca agatgtcggc aatcgcaatc attttttccc
     6181 cttagtacga tgaacagcgg taaagaaatc gtattcttta tgcgtcataa cttcacgtat
     6241 gtagcacttt tgcgattcaa aaaagaccat tgctacaaca cgtaattcat tgcccccaac
     6301 attgaaaaca taatgcttat ccagatattt gaagttatcc agagatggga atactgcttt
     6361 taatgactca ggttttttga aatatccctt agcaatcgtg ttccccagag ccaccaactc
     6421 cgttttatgt tgcgggtatt tttccgcagc atctttcaat gctttttgag ttatcaggtg
     6481 cattcttcat cacgtccgtt gacaaattgg caatatgata acatccgttg acagattggc
     6541 aacggatgaa tttatctgtg tgacggaaga ataaacgtag aaaaacgcta aacaagagaa
     6601 tgtagaggat ggtgagaggg attttttgtt ttatggaagc gcgattccag aactgggcag
     6661 acattgccgg atgcgacgct tgccgcgtct tatccggcct gggagtaacg tgctgaacgt
     6721 aggccggata aggcgttaac gccgcatccg gcaggtaact cccaatctta ccgacgacgc
     6781 cccagcttca ctgctttcag caccacaaat ttattattcg tggcgatggt ggtgcagttg
     6841 ccgaaaatct tcttcagttt atggaagtaa tccaggtgac ggttggcaac aatatacagc
     6901 tcgccgttga ttttcaggca gcggcgggca tggtggaaca tctcccaggc gacgttatcg
     6961 gtcagcgcat gttgttggtg aaacggcggg ttgcagagca cagcattaaa gcggaaaggc
     7021 tccacgccgg agagcgcgtt gttgatcata aactcgctgc gatctaacgc ctctggcatg
     7081 ttggtttcaa cgttcaaacg gctggaagca accgccatcg gcgattcatc gacaaacacc
     7141 actttcgcct gagggttttt atcaagcagc gtcagaccaa taacgccatt accgcaaccg
     7201 agatcgacaa tctcaccttc gagattctct ggcagatgtt gcataaagaa gcgtgcgcca
     7261 atatccagcc cggtgcggga gaagacattc gcatggttgt ggatagtcca gtcagtacct
     7321 tccagcttcc agctaacggt ctgcggcgca tcggccagcg gcggttcatt gaaagtgcaa
     7381 ttaatcaggc gcgctttttt ccatgccagc gtagtggtgg tcgggccgag cactttctcg
     7441 aacagctcca gcgtggaagt gtgaatgtca cgggctttgg caccggcaat aatacgtgta
     7501 tccgacgtga ccactttgcg cagcgcacgc agttgctgtt ccagcaatgc cagtgttttc
     7561 ggcactttga tcagcaccac acccggctgt tgcgggtagt cggcggtgct gtcgagaaac
     7621 ttcacgctcg actcatcaat cccgttgagg cgtaaattct cgcgcgtcgc cagttcactg
     7681 atgtatgagt cgccaatgct gtacggctta tgttccgcca gcgcgcagct taacgcacca
     7741 aaggcatcat tcaggatcaa caccgggccg cggatttctg tgtcgtccaa ctgttgcagc
     7801 aaatattcat ccgccgcttc ccacgcctgt agcgggttaa cgtcatccgt cgccggaaaa
     7861 cgttgtagtg tcagtgaacg gaaaccgttg tctaagtggc tcatcggccc tcctgaatga
     7921 taaaatttcg acgttgcccc tgaaaagggt gcgtgagtat accctttttc tctttttcgt
     7981 gggcggttat gagcaatctt acttatcttc agggttatcc cgagcagcta ctttcccagg
     8041 tgcgaacgct gattaacgaa cagcgtctgg gcgatgtgct ggcaaaacgc tatccgggaa
     8101 cacacgacta cgccaccgat aaagccctct ggcaatatac ccaggatcta aaaaatcagt
     8161 ttctgcgcaa tgccccgccg atcactaaag tgatgtatga caataagatc cacgtactga
     8221 aaaacgcgct cggattacat accgctgttt ctcgtgtgca gggcggcaag ctgaaagcaa
     8281 aagcagagat ccgcgtcgct actgtgtttc gcaacgcgcc ggaaccgttt ttacgcatga
     8341 tcgtggtgca cgaactggcg cacctgaagg agaaagagca taacaaagcg ttttaccagt
     8401 tgtgctgcca tatggagccg cagtaccacc agcttgagtt cgacacccgg ctgtggctaa
     8461 cgcagttgtc gcttgggcag gacaaaatct gaaaatgatg tcaggcgtta tcttttcttt
     8521 ctttagcgcc taaaatcgac ctcccccctt tcgttaagga taacgaccat gcagccccgc
     8581 cttcttctcc gtatttgttt ttctcgccgg actctaaaga ttacctgctt actgttactt
     8641 gtggcaggag cgaccatttt tatcgccgat cgcgtaatgg tcaacgccag caagcagctg
     8701 acctggagcg atgtcaacgc agttccggcg cgtaacgtgg gtttattgct gggggcgagg
     8761 ccgggtaatc gctactttac ccgacgtatt gataccgcag cagcgttgta ccacgtcgga
     8821 aaagtgaaat ggctgttagt cagtggcgat aacggacgta aaaattacga tgaagcctca
     8881 ggtatgcagc aggctttgat cgctaaaggc gtaccggcga aagtgatctt ctgcgactat
     8941 gccggattct caacgcttga ttcagtggtg cgtgccaaaa aggtgtttgg ggaaaaccat
     9001 atcaccatta tctcgcaaga atttcataac caacgcgcca tctggctggc aaaacagtac
     9061 ggcatcgacg ctatcgggtt taacgcgcca gaccttaata tgaagcacgg tttttatact
     9121 caactgcggg agaaactcgc tcgtgtcagc gcggtgattg acgccaaaat cctccatcgc
     9181 cagccgaaat atctcgggcc atcggtcatg attgggccgt ttagtgagca tggctgtccg
     9241 gcgaaagaat aatgcgtatc tgcgcacgtc gaagatgaaa aaggcgtgct acattgacga
     9301 cagaatccct ttatggagta tccacgcgtt atgatacgtt tcgctgtgat tggtacgaac
     9361 tggatcactc gccagttcgt cgaggccgcc catgagagcg gtaaatacaa gttaaccgcc
     9421 gtatattccc gcagccttga acaggcccag cacttcgcca atgatttttc tgtcgagcat
     9481 ctgtttacct cgctggaagc gatggcggaa agcgatgcca ttgacgcggt gtatattgcc
     9541 agcccgaatg ccctgcactt ttcccagaca caacttttcc ttagccataa aattcatgtg
     9601 atttgcgaga aaccactggc gtcgaatctg gcggaagtgg atgccgccat tgcctgtgcg
     9661 cgggaaaatc aggtggtgct gtttgaggca tttaaaaccg ccagcctgcc gaactttcat
     9721 ttgctgcgcc aggcgctgcc gaaagtcggc aaattgcgta aagtcttttt caactattgc
     9781 cagtattcct cgcggtatca acgttacctg gatggtgaga atcccaacac ctttaatccg
     9841 gcattctcta acggttcaat tatggatatc ggcttttact gcctggcgtc ggcggtggca
     9901 ttattcggtg aaccgaaaag cgtgcaggca accgccagtt tgttggcaag cggcgtagat
     9961 gcccacggcg tggtggtgat ggattacggt gatttcagcg ttacgttgca gcactcaaag
    10021 gtcagtgatt ctgtcctgga aagcgagatt cagggcgaag cgggatcgct ggtgattgaa
    10081 aaactgtctg aatgccagaa agtgtgcttc gtgccgcgtg gcagccagat gcaggatctc
    10141 acccagccgc agcatattaa taccatgctc tacgaagcag agctgtttgc taccctggtg
    10201 gatgagcatc tggtggatca tccagggctg gcggtcagtc gcatcaccgc caaactgctg
    10261 actgagatcc gccgccagac tggggtgatt tttccggcag atagcgtaaa actataattg
    10321 ccaaagtaaa acagtgtaaa aggtatgtaa cagaccattg actggctgaa tggtctgtca
    10381 tactttgtta cctgcaaagg ggagtaactt cattgccggt cgatcgtcat tacgatgtgt
    10441 gaaaaaccac atccggtcac cgggcaaccc gaaaggaata cgcagacgta ttcctttttt
    10501 gttgtaagtg agaccttgcc ggaaggcgag gtctatgcat aaaaagcagc ggctgacgtc
    10561 ttccgacgtt ggccgttttt ttatgtgtaa ggaacttcta tgaatactgt cggcacgccg
    10621 ttgctatggg gcggattcgc tgttgttgtc gccattatgc tggctatcga cctgttgttg
    10681 caggggcgtc gtggggcaca tgccatgacc atgaaacagg ctgcggcctg gtcgctggtc
    10741 tgggtgacgc tgtcgttact gtttaacgcc gctttctggt ggtatctggt gcaaactgaa
    10801 ggtcgcgccg ttgccgatcc acaggcactg gcctttctca cgggttatct gattgagaaa
    10861 tcgctggcgg tcgataacgt ctttgtctgg ctgatgttgt tcagctattt ctctgttccg
    10921 gcggcattac aacgccgcgt gctggtgtat ggcgtgctcg gggcgattgt tctgcgtacc
    10981 atcatgatct tcactggcag ctggctaatt tcgcagttcg actggatcct gtatatcttc
    11041 ggtgccttcc tgctgtttac cggcgtgaag atggcgctcg cccatgaaga tgaatcaggc
    11101 attggcgaca agccgctggt gcgctggcta cgcggtcatt tgcgcatgac cgacaccatc
    11161 gacaacgagc atttctttgt gcgtaagaat ggattgctgt acgccacacc gctgatgctg
    11221 gtactgattc tggtggaatt gagcgacgtg attttcgccg tggatagcat tccggctatc
    11281 ttcgccgtga ccactgaccc gttcattgtg ctgacctcaa acctgtttgc gatcctcggc
    11341 ctgcgtgcga tgtatttcct gctggcgggc gtagcagagc gtttctcgat gctcaaatat
    11401 ggcctggcgg tgattctggt gtttatcggt atcaagatgc tgattgtcga cttctaccat
    11461 attccaatcg ccgtctcgct gggcgtggtg tttggcattc tggtgatgac gtttattatc
    11521 aacgcctggg tgaattatcg gcatgataag cagcggggag aatgattcgc tgtatattaa
    11581 gccattccta gagataactc attccagaca aaggtgagct attaattatg taatcggctt
    11641 gtagctcttt acaaaaagac cgcacgggtg ttggtggcgt caaaatcgcc gaagcgttcc
    11701 ctggaggccg gggcgacgcg catggatgcg cggcgagggc gcatcttaca gggacgttcc
    11761 cttgcgcccg acccggtagc cgcaagggat aagctgaggg aatcgcgcag cggcgatttt
//
LOCUS       DAZOZY010000123        11778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZOZY010000123 DAZOZY010000000
VERSION     DAZOZY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.123"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHURQ_002858"
     CDS             complement(<1..611)
                     /locus_tag="ACHURQ_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR0824585.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHURQ_002859"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHURQ_002859"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0824586.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHURQ_002860"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHURQ_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR0824587.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHURQ_002861"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHURQ_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR0824588.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHURQ_002862"
     CDS             complement(5085..6716)
                     /locus_tag="ACHURQ_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR0824589.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHURQ_002863"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHURQ_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR0824590.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHURQ_002864"
     CDS             7139..7411
                     /locus_tag="ACHURQ_002864"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0824591.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHURQ_002865"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHURQ_002865"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR0824592.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHURQ_002866"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHURQ_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR0824593.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHURQ_002867"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHURQ_002867"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR0824594.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHURQ_002868"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHURQ_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR0824595.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11778)
                     /locus_tag="ACHURQ_002869"
     CDS             complement(11524..>11778)
                     /locus_tag="ACHURQ_002869"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR0824596.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3044 a         2857 c         2892 g         2985 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtaa aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggt
//
LOCUS       DAZOZY010000124        11699 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZOZY010000124 DAZOZY010000000
VERSION     DAZOZY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.124"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHURQ_002870"
     CDS             197..301
                     /locus_tag="ACHURQ_002870"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR0824597.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHURQ_002871"
     CDS             complement(350..1603)
                     /locus_tag="ACHURQ_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0824598.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHURQ_002872"
     CDS             1710..2603
                     /locus_tag="ACHURQ_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0824599.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHURQ_002873"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHURQ_002873"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR0824600.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHURQ_002874"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHURQ_002874"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR0824601.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHURQ_002875"
                     /pseudo
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHURQ_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHURQ_002876"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHURQ_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR0824602.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHURQ_002877"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHURQ_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR0824603.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHURQ_002878"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHURQ_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR0824604.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHURQ_002879"
     CDS             complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHURQ_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR0824605.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSYTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(10807..>11699)
                     /locus_tag="ACHURQ_002880"
     CDS             complement(10807..>11699)
                     /locus_tag="ACHURQ_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR0824606.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2959 a         2963 c         3021 g         2756 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcggccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgctagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccacaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa atctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt ccgtaccatg
     9421 gtcgatggca tcagtccatg taaagacgga aatctgcgtt ttaacaggat tctcgagcat
     9481 cgggaaccat tctaccccca gatcccagct accttcgcgt acgcctgagt tgccgccgtt
     9541 tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg tttgttcgcc
     9601 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
     9661 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
     9721 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
     9781 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
     9841 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
     9901 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
     9961 acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    10021 agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    10081 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    10141 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac caccgcaaga
    10201 gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc
    10261 gtacaggaca tgtacagctt cattgccgta atcttttagg atccgccgaa gattatcact
    10321 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc
    10381 gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc ggatagagcg
    10441 cccgcgtaaa cacgcgcgaa cctgatgatt accgtagacg tcgtcacctg tcgtatcata
    10501 ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc ggctcccgca
    10561 gttaacggtg taggaactcc agaccgcttt ctcttctacc ggagcctctg ccgcccgaac
    10621 catttgggaa aatggcagag tgaaagcact gcttgccagc gcaaggctgc caagtgcgga
    10681 ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg tggtatggat
    10741 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    10801 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    10861 tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccatggtcga ttttatcgcc
    10921 agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag ggagaatgcg
    10981 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    11041 gccggtggca atcccacgtt tctgtgcatc tatagggttg atccacactt cctgacggca
    11101 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    11161 accaaacagt tgcagtggga aggtactacg ttcaggggag tcccagcctt caaaggttga
    11221 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    11281 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    11341 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat ctttacgctt
    11401 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    11461 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga acttttgata
    11521 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc
    11581 gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac cggcgtagtc
    11641 gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa tgtcggcat
//
LOCUS       DAZOZY010000125        11609 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZOZY010000125 DAZOZY010000000
VERSION     DAZOZY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.125"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            294..1682
                     /gene="narU"
                     /locus_tag="ACHURQ_002881"
     CDS             294..1682
                     /gene="narU"
                     /locus_tag="ACHURQ_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR0824607.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1764..5504
                     /gene="narZ"
                     /locus_tag="ACHURQ_002882"
     CDS             1764..5504
                     /gene="narZ"
                     /locus_tag="ACHURQ_002882"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR0824608.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5501..7045
                     /gene="narH"
                     /locus_tag="ACHURQ_002883"
     CDS             5501..7045
                     /gene="narH"
                     /locus_tag="ACHURQ_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR0824609.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7045..7740
                     /gene="narW"
                     /locus_tag="ACHURQ_002884"
     CDS             7045..7740
                     /gene="narW"
                     /locus_tag="ACHURQ_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR0824610.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7737..8417
                     /gene="narI"
                     /locus_tag="ACHURQ_002885"
     CDS             7737..8417
                     /gene="narI"
                     /locus_tag="ACHURQ_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR0824611.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYACFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8496..9389
                     /locus_tag="ACHURQ_002886"
     CDS             8496..9389
                     /locus_tag="ACHURQ_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR0824612.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9485..10330)
                     /gene="nhoA"
                     /locus_tag="ACHURQ_002887"
     CDS             complement(9485..10330)
                     /gene="nhoA"
                     /locus_tag="ACHURQ_002887"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR0824613.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10503..11072
                     /locus_tag="ACHURQ_002888"
     CDS             10503..11072
                     /locus_tag="ACHURQ_002888"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR0824614.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11076..11303)
                     /gene="pptA"
                     /locus_tag="ACHURQ_002889"
     CDS             complement(11076..11303)
                     /gene="pptA"
                     /locus_tag="ACHURQ_002889"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR0824615.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(11403..>11609)
                     /locus_tag="ACHURQ_002890"
     CDS             complement(11403..>11609)
                     /locus_tag="ACHURQ_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0824616.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         2717 a         3012 c         3056 g         2824 t
ORIGIN      
        1 tctggtcttg ttgataatat agttaatatg taacccttta gattcacgat ctgaagggtt
       61 gaaacatgac atacgttcaa agattaaccc cgtttgaagg ttagcttgtt agttacattt
      121 agtaacacat atctggctac ttaatcccca ttctgccccc gcggttacgc tttcttatct
      181 attctttcaa tcagcacttc acccaattca tccttaattc taataactgg ctattgcgcc
      241 ttacctcctg ttttgtcttt tttcattacc aatgtgtgca tgtgaggaac aatatggcac
      301 tgcaaaatga gaaaaatagt cgttatcttt tacgcgactg gaaaccagaa aatccggcct
      361 tctgggaaaa taaaggaaaa catattgctc gaagaaacct ctggatatca gtcagttgtc
      421 tacttcttgc cttctgtgtc tggatgctat ttagcgcagt taccgttaat ctcaataaaa
      481 tcggttttaa ttttactacc gatcaactct ttttattaac cgcattaccc tccgtttctg
      541 gcgcattatt gcgtgttccc tactccttta tggtgcctat attcggtgga cgccgatgga
      601 cggtttttag tactgcaatc ctgattattc cttgcgtctg gctcggaatt gccgtgcaaa
      661 atccgaatac tccttttggg atatttatcg ttatcgcttt gctatgcggt tttgcaggtg
      721 caaactttgc ttcgagcatg ggcaatatca gtttcttctt tccaaaagcc aaacaaggga
      781 gcgctcttgg gattaatggc ggattaggaa acttaggtgt aagtgtaatg cagctggttg
      841 caccgctggt catttttgta cctgtatttg cctttctcgg cgtcaatggc gtaccgcagg
      901 ccgacggttc ggtgatgtcg ctggcgaatg ccgcatggat ttgggtaccg ctactggcga
      961 ttgccacgat cgccgcctgg tcagggatga atgatatcgc cagttcacgc gcctcaattg
     1021 acgaccagct ccctgtctta caacgcctgc atctctggct gctgagcctg ctttaccttg
     1081 ccaccttcgg ttcgtttatc ggtttttctg cgggttttgc catgctggca aaaacccagt
     1141 tcccggatgt gaatattctg cgcctggcgt tctttggccc atttatcggt gccatcgcgc
     1201 ggtcggttgg tggtgctatt tccgataaat tcggcggcgt gcgggtgacg ttgatcaact
     1261 ttatttttat ggcgattttc agtgccctgc tgttccttac cttaccgggc acaggctccg
     1321 gtaatttcat cgccttttac gccgtattta tggggctgtt tctgactgcg ggtctgggaa
     1381 gtggttctac tttccagatg atcgccgtca tctttcgcca gataaccatt tatcgggtga
     1441 agatgaaagg cggtagtgat gagcaagctc agaaagaagc catcaccgaa acggcggcgg
     1501 ctctgggctt tatctcagcc attggcgcag tgggcggctt ttttattccg caggcgtttg
     1561 gcatgtcgct caatatgacc ggctctccgg tcggcgcgat gaaagtgttt ttaatcttct
     1621 acatcgtttg tgtgctgctg acctggctgg tttatggtcg gcggaagttc agccaaaaat
     1681 aagagatcga tgcctgatgt acaaccgtgt cgcatccgac aaggcatcag atcccaattc
     1741 attcctggag caggagtcat gtcatgagta aacttttgga tcgctttcgc tacttcaaac
     1801 aaaagggcga aacctttgcc gatggtcacg gacaggtgat gcatagcaac cgcgactggg
     1861 aggacagcta tcgccagcgt tggcagttcg acaaaatcgt gcgttccacc cacggtgtta
     1921 actgtacagg ctcctgtagc tggaaaatct acgttaaaaa tggtctggtg acctgggaaa
     1981 tccaacagac cgactacccg cgcactcgcc ctgaccagcc caatcatgaa cctcgcggct
     2041 gcccgcgtgg cgcaagttac tcctggtatc tctacagcgc taaccgcctg aaatacccgc
     2101 tcattcgtaa acgactgatt gaactgtggc gtgaagctct caagcaacac agcgatccgg
     2161 tactggcgtg ggcatcgatt atgaacgatc cgcaaaagtg cttaagctac aaacaagtgc
     2221 gtgggcgcgg cgggtttatc cgctccaact ggcaggaact taaccaactg attgccgccg
     2281 ctaacgtctg gaccatcaaa atctatggtc cggatcgcgt tgccggtttc tcgccgatcc
     2341 cggcgatgtc gatggtgtca tacgctgccg gaacgcgtta tctgtcgctg ttaggcggca
     2401 cctgtttaag cttctacgac tggtattgcg acctgccgcc cgcctcgccg atgacctggg
     2461 gcgaacaaac cgacgtgccg gaatctgccg actggtataa ctccagttac atcatcgcct
     2521 gggggtctaa cgtaccgcag acacgtacgc cggacgccca cttctttacc gaagtacgtt
     2581 acaaaggcac caaaactatc gccattaccc ctgactactc tgaagtggcc aaattgtgcg
     2641 accagtggct ggcaccgaaa caaggcactg atagcgccct ggcgatggca atgggccatg
     2701 tgattttaaa agagtttcat ctcgataatc ccagcgacta ctttatcaac tactgccgcc
     2761 gctatagcga catgccgatg ctggtaatgc tggaacctcg cgacgatggt agctacgtac
     2821 ccgggcgcat gatccgcgcg tctgacctgg tagatggact aggcgaaagc aataatccgc
     2881 agtggaaaac cgtggcggtt aataccgcag gtgaattggt agtgccgaat ggttcgattg
     2941 gtttccgctg gggagaaaaa ggcaaatgga atctggaatc cattgccgcc ggtaaggaaa
     3001 ccgaattgtc gttaaccctg ctcggtcaac atgacgctgt tgcaggcgtg gccttcccct
     3061 actttggcgg cattgaaaat ccgcattttc gcagcgtaaa acacaatccg gtgctggtgc
     3121 gccaattgcc cgttaaaaac ctgacgttag tcgatggcaa cacctgtccg gtggtcagcg
     3181 tttatgattt ggtgctggcg aattacggcc tcgatcgcgg gctggaagat gaaaacagcg
     3241 cgaaagatta cgctgaaatc aaaccgtaca ccccagcctg gggtgagcaa attaccggcg
     3301 taccgcgcca gtatattgaa accatcgccc gcgaatttgc cgataccgcc cataaaacgc
     3361 atggacgctc aatgattatc ctcggcgctg gcgtcaacca ctggtatcac atggatatga
     3421 actaccgtgg gatgatcaat atgctgatct tctgcggttg tgtcgggcaa agcggcggcg
     3481 gctgggcgca ctatgtcggt caggaaaaac tgcgcccaca aaccggctgg ttgccgctgg
     3541 cctttgcgct cgactggaac cgcccaccgc gccagatgaa cagtacctcg tttttctaca
     3601 atcattccag ccaatggcgc tatgaaaaag tctctgctca ggagttactt tcaccgcttg
     3661 ccgatgccag taagtacagc ggtcatctga ttgatttcaa cgttcgcgcc gaacgcatgg
     3721 gctggctacc ttctgcgccg cagctggggc gtaacccgct cgggattaaa gctgaagccg
     3781 acaaggccgg attatccccc acagaattta ccgcccaggc gctgaaatcg ggcgatttac
     3841 gtatggcctg cgaacaacca gatagcggca gcaatcatcc gcgtaatttg tttgtctggc
     3901 gctctaacct gcttggctcc tccggcaaag gccacgagta tatgcagaag tatctgctgg
     3961 ggaccgaaag cgggattcag ggcgaggaac tcggtgccag cgacgggatc aaaccggaag
     4021 aagtcgagtg gcaaactgcg gcgattgaag gcaagctcga cctgctggtg acgctcgact
     4081 tccgcatgtc cagtacctgc ctgttctccg atatcgttct gcccaccgcc acctggtacg
     4141 aaaaagacga tatgaacacc tcggatatgc atccgtttat tcatccgctt tctgcggcgg
     4201 tcgatccggc gtgggaatca cgcagcgact gggaaatcta caaaggtatc gccaaagcat
     4261 tttcgcaagt gtgcgtgggt catcttggca aagaaaccga cgtggtatta caacccctac
     4321 tgcatgactc tccggcagag ctctcacagc cgtgtgaagt gctcgactgg cgcaaaggcg
     4381 aatgcgatct gattccaggc aaaaccgcgc cgaatattgt ggcggtggag cgcgactacc
     4441 ctgctacgta tgaacgcttt acctcgctcg ggccattgat ggacaaactt ggcaatggtg
     4501 gtaaagggat ttcgtggaat acccaggatg aaatcgattt cctcggcaaa ctcaattaca
     4561 ccaagcgtga tggcccggca caggggcgtc cgctgattga caccgccatt gacgcttcag
     4621 aagtgattct ggcactggca ccggaaacca acggtcatgt tgcagtcaaa gcgtggcagg
     4681 cgctgggcga gatcaccggg cgcgaacata cccatctggc gctgcacaaa gaggacgaga
     4741 agattcgctt tcgcgatatt caggcgcagc cgcgtaaaat tatctccagc cccacatggt
     4801 ccggtctgga aagcgatcac gtctcctata atgcgggata caccaacgtt catgagttaa
     4861 ttccgtggcg cacgctgtcg ggacgccagc agctctatca ggatcatccg tggatgcgtg
     4921 cttttggtga aagcctggtg gcttatcgcc cgcctatcga cacccgtagc gtcagtgaga
     4981 tgcgccagat cccgccaaac ggcttcccgg aaaaagcact taacttcctg acgccgcacc
     5041 agaaatgggg cattcactca acctacagtg aaaacctgct aatgctgacg ctctctcgcg
     5101 gtggaccgat tgtctggatc agcgaaaccg atgcccgaga actgaccatt gtcgataacg
     5161 actgggtgga agtctttaac gccaacggcg cgctgacggc tcgtgcggtg gtcagccaac
     5221 gtgtaccgcc gggtatgacc atgatgtatc acgcccagga acgcattatg aatattcctg
     5281 gttcggaagt aactggcatg cgcggcggca ttcataactc ggtcacccgt gtttgcccga
     5341 aaccgacgca tatgattggc ggttacgcgc agctggcctg gggctttaac tactacggca
     5401 ccgtcggctc gaaccgcgac gaattcatca tgatccgcaa gatgaagaac gttaactggc
     5461 tggatgatga aggtcgcgat caggtacagg aggcgaaaaa atgaaaatac gttcgcaagt
     5521 cggcatggtg cttaacctcg acaaatgtat cggttgccat acctgttcgg tgacctgtaa
     5581 aaacgtctgg accggacgcg aaggcatgga gtacgcatgg tttaacaacg tcgaaaccaa
     5641 accgggcatt ggttatccga aaaactggga agatcaggaa gagtggcaag gcggctgggt
     5701 ccgcgatgtg aatggcaaga tacgcccgcg tctgggcagc aagatgggcg taataaccaa
     5761 aatcttcgcc aacccggtgg tgccgcagat tgatgattac tacgaacctt tcaccttcga
     5821 ctacgaacat ttgcatagcg caccggaagg caaacatatc cctactgctc gcccgcgttc
     5881 gctgattgac ggtaagcgga tggacaaagt gatctggggg ccaaactggg aagaactgct
     5941 gggcggcgag tttgaaaaac gtgcccgcga ccgcaacttc gaggccatgc aaaaggagat
     6001 gtacgggcag tttaaaaaca ccttcatgat gtacctgccg cgcctgtgcg aacactgcct
     6061 caatcccagt tgcgtggcga cctgcccaag cggcgctatc tacaaacgcg aagaagacgg
     6121 cattgtgctg attgatcagg ataaatgccg tggctggcgt ttgtgcatta gcggttgtcc
     6181 gtacaaaaaa atctacttca actggaaaag cggcaagtca gaaaaatgta tcttctgtta
     6241 tccgcgaatt gagtcaggcc aacccaccgt gtgctcagaa acctgcgtgg gtcgcatccg
     6301 gtatctgggc gtgctgcttt acgacgccga ccgcattgag gaagcggcga gcaccgagcg
     6361 cgaagttgac ctctatgaac gtcagtgcga agtgttcctc gatccacacg atccctcagt
     6421 gatcgaggaa gccctgaaac aaggtattcc acaaaacgtg attgaagctg cccagcgttc
     6481 gcctgtctac aaaatggcga tggactggaa actggcgcta ccgctgcacc ctgaatatcg
     6541 caccctgcca atggtctggt acgttcctcc gctgtcgccg attcagtcct acgcagatgc
     6601 gggcggtttg ccgaaaagcg aaggcgtgct gcccgccatc gaaagcctgc gtattccggt
     6661 gcaatatctc gccaatatgt tgagtgccgg cgataccggt ccggtactgc gggcgctgaa
     6721 acggatgatg gcgatgcgcc actatatgcg ttcacaaacc gtggaaggcg ttactgatac
     6781 tcgtgccatc gacgaagtag gcctgagcgt cgcccaggtc gaagaaatgt atcgctatct
     6841 cgccattgcc aactatgaag atcgttttgt tatcccgacc agccatcggg aaatggcggg
     6901 cgatgccttc gcagaacgca acggctgcgg ttttaccttt ggcgacggtt gccacggttc
     6961 ggacagtaaa ttcaacctgt tcaacagtag ccgtatcgat gccatcaaca tcaccgaagt
     7021 gcgcgacaaa gcggagggcg aataatgcag atcctcaaag tgatcggcct gttgatggag
     7081 tatccggatg agctgttgtg ggaatgcaag gaggacgcgc tggcgttgat ccgccgcgac
     7141 gcgccgatgc ttacggattt cacccacaac ctgcttaacg cgccgctgct ggataaacag
     7201 gccgaatggt gcgaagtgtt tgaccgcggg cgcaccacgt cgctgctgct gttcgaacat
     7261 gttcatgccg agtcccgcga tcgcggccag gcaatggtgg acctgctggc ggagtatgaa
     7321 aaggtcggcc tgcagctgga ttgtcgggaa ctgcccgatt atctaccgct gtatctggag
     7381 tatttaagcg tactgcctga cgatcaggcg aaagaaggat tgctcaatgt tgcgccgatc
     7441 ctcgccctgc ttggcggtcg cttaaaacaa cgcgaggcac cgtggtacgc gttgtttgat
     7501 gctctgctgc aactggcggg aagtatactt tcaagtgaca gtgtcacaaa acaagtaaac
     7561 agcgaagagc gcgatgacac ccgccaggcg cttgatgcag tgtgggaaga ggaacaggtg
     7621 aagtttattg aagataacgc cacggcgtgt gacagctcgc cgcttaatca atatcagcga
     7681 cgctttagcc aggatgtcgc gccgcagtat gtcgacatca gtgcgggagg tgggaaatga
     7741 ttcagtatct gaacgtcttt ttttacgata tctacccgta catttgtgcg acggtgtttt
     7801 tcctcggcag ctggctgcgc tacgactacg ggcagtacac ctggcgcgcc tcctcaagtc
     7861 aaatgctcga taaacgcggg atggtgatat ggtcgaatct gttccatatc ggcattttgg
     7921 ggattttctt cgggcacttg ttcggcatgt taacgccgca ctggatgtac gcgtgttttt
     7981 tgccagtggc agcgaaacaa ctcatggcga tggtgctcgg cggtatttgc ggtgtgttga
     8041 cgttgattgg cggcgcagga ctgctgtggc gcagtctgac aaatcagcgc gtgcgtgcca
     8101 cttccactac gccggatatc atcatcatga gcattttgct gatccagtgt ctgttggggc
     8161 taagtaccat accgttttca gcacagtatc ctgatggtag cgaaatgatg aagctggtgg
     8221 gttgggcgca aagcattgtc actttccgtg gtggatcgtc agagatgctc aacggcgtag
     8281 cgttcgtctt ccgcctgcat ctggtgttgg gaatgaccat cttcctgctc ttcccgttca
     8341 cccgactggt gcatgtgtgg agcgcgccgt ttgagtactt tactcgtcga tatcaaattg
     8401 tgcgttcgcg gcgataattt caccctggcc ggatagccct tcgctctccg gccacttgca
     8461 ggctatgata gttctccagt cacaggagaa cgttaatgaa accgcaggtg taccacgtcg
     8521 atgcttttac ctctcaacca tttcgcggca attctgccgg tgttgttttc cccgccgata
     8581 atcttagcga agcacaaatg cagcttatcg cccgcgagtt aggccattcg gaaactgctt
     8641 ttctgctgca cagcgacgac agcgatgtgc gcatccgcta ctttacgcca acggttgaag
     8701 tgccgatttg cggtcacgcg acagtagctg cgcactatgt acgtgcgaag gtgttaggtt
     8761 taggaaattg cacgatctgg caaacatcgc tggcaggaaa acatcgcgtg actatcgaaa
     8821 agcacaacga tgattatcgt atttcgctgg aacaaggtac gccgggcttt gaactgccgc
     8881 tggagggtga agtacgcgcg gcgattatca acgcattgca tcttactgaa gacgatattc
     8941 tgccgggttt gccgatccag gtcgcgacga ccgggcattc taaagtgatg atcccattga
     9001 agccggaagt ggatatcgac gctctttcgc ctgaccttaa tgcgctgacc gctatcagta
     9061 aacagattgg ctgcaatggt ttcttcccgt tccagatccg tccaggcaaa aacgaaaccg
     9121 atggtcgtat gttctcgcct gcgattggta ttgtggaaga tccggtgact ggcaatgcca
     9181 atggcccgat gggtgcatgg ttggtacatc acaacgtatt gccccacgat ggcaacgtgt
     9241 tgtgtgttaa aggccatcag gggcgcgcat tggggcgtga cggcatgatt gaagtgacgg
     9301 tgacaattcg tgataaccaa ccggaaaaag tcaccatttc aggcacagcg gtgattttgt
     9361 ttcatgccga atgggcaatt gaaatctgaa atcgttgcct gatgcgcttc gcctgtcagg
     9421 tctacaaccg ggctaatgta tgccggataa ggcgtttacg ccgcatccgg caacctgaca
     9481 taaattattt tcccgcctcc ggatgcgtat caaacgccgc catcaccagc gctaactcat
     9541 ccacagtaaa gccatgtttc gcatcatcca cgcccagacc aaactgttcc tgcatcacgg
     9601 catataacga cgccacatcc ggtagatttc gctgctccac cgcgtgccca ttttcataat
     9661 gggtaaaatg aaaattggtc agtgtcagct tgccgccgtc cggcaaatgg cggcacatca
     9721 gcaaatgatg gcgaaaatgc gactgcggcc agtgcgccga ccagaaattg cccatcacat
     9781 aatcgctttg ttgctgctcg cagagatcga agcggtacat cggctgccaa tgctgatgat
     9841 gattaaactg caacacccaa tcatcaccct cctgcaacaa ccgatactct ccgtgtggcg
     9901 tggtctgcac gagatcggaa actaaacgaa tcggcgcggt tagcgtctgc ccaccgaaac
     9961 cgacatcagc aatccatttt tccccttcca gttccaccaa cagcaaacga tgggtgcgcg
    10021 gcggtaatgc tggcggattt gataacacta cgcgccctaa caagctgcga acgttaaacc
    10081 ccagctcgcg taacacccgc tcaaacacgc cattctgctc aaaacagtaa ccgccacgac
    10141 gggctatcac cagtttctct tccagcgatt gatcatcaag ctgtatttcc ctcggcagca
    10201 aaacgtcaag gttttcaaac ggaatggtgc aattgtgttt caggtgcaat gcacgaagcg
    10261 tatcaatatt gaccgcagca gctcccgacc agttaatacg ggcaaaatag tgattcagaa
    10321 tgggcgtcat caggcgtttc ccttagtggt tttctcaact tataacctgg aatagacgcc
    10381 gcaccggcta ttttgtgcga tcgcggacat gcatcacgtt tgcaaatcgc caatcccgtt
    10441 aatttcgtta cttattgcgt tacggactac gcttaaaaaa accttcttcg cgaggcgata
    10501 ccatgagccg attcatcccc atagaattac accatgccag ccgtctgtta aatcatggcc
    10561 caactgtctt gatcaccagt ttcgatgagc aatcccagcg gcgtaacatt atggctgcag
    10621 cctggtcaat gccggtggag tttgaacctc cacgcgtggc aattgtggta gataaatcga
    10681 catggaccag ggagttgatt gaacgtaacg gtaagtttgg catcgttatc ccgggcgttg
    10741 cagcaactaa ctggacgtgg gcggtgggaa gtgtgtcggt gcgtgaagaa gataaattta
    10801 attgctatgg cattccggtg gtgaaaggtc cggtgcttgg tttgccactt gttgaagaga
    10861 aatgcctggc gtggatggag tgccgcctgc tacctgcgac ttctgcgcaa caaaaatacg
    10921 acacgctgtt tggcgaggtg gtatcagcag cggcagacgc acgggtattt gtcgaaggcc
    10981 gctggcagtt tgatgatgat aagctcaata cgttgcatca tttaggtgct ggcacgtttg
    11041 ttactagcgg caagcgtgtt acggcaggtt aagcattaca tgctgtaacc gggtttcttt
    11101 atcaaagcct ccatttgggg ctcgatttcg gcatcccaga tagcttgcca agattctggt
    11161 tgaatctgct gtagagcaat gcttatcgag ctatctttac tgttcagatg gcgaataata
    11221 acgtcggtaa tatctgcagc aagtgctgct ttttgttgtt cgtccagttc acgcggaaag
    11281 catttaatgt cgatgtgcgg catgattacg ctccttttga aagatgtcag taaaattaac
    11341 acacattgtg aatgtcatgt gtccggtaaa gcgtttatac gacaataatg cccagggcaa
    11401 gattacgaaa gcccgctccc cgtaaggact gacgccaggt agtttctgcc catggctgct
    11461 tttcgcatct tacgtcttaa ccctgccttg aataccttat cattcgtcaa aatattaata
    11521 gcaatgtgcc gtatccctga aaataattct gctgcatttc ctcttcttat tttgcagtcg
    11581 tcttcattca ttaccacgtc cagacgcca
//
LOCUS       DAZOZY010000126        11542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZOZY010000126 DAZOZY010000000
VERSION     DAZOZY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.126"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHURQ_002891"
     CDS             460..1386
                     /locus_tag="ACHURQ_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR0824617.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHURQ_002892"
     CDS             complement(1336..2529)
                     /locus_tag="ACHURQ_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0824618.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHURQ_002893"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHURQ_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR0824619.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHURQ_002894"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHURQ_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR0824620.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHURQ_002895"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHURQ_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR0824621.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHURQ_002896"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHURQ_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR0824622.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHURQ_002897"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHURQ_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR0824623.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHURQ_002898"
     CDS             complement(11165..11308)
                     /locus_tag="ACHURQ_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824624.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2926 a         2896 c         2950 g         2770 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAZOZY010000127        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZOZY010000127 DAZOZY010000000
VERSION     DAZOZY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.127"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHURQ_002899"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHURQ_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR0824625.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHURQ_002900"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHURQ_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR0824626.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHURQ_002901"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHURQ_002901"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR0824627.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHURQ_002902"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHURQ_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR0824628.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHURQ_002903"
     CDS             complement(3928..5214)
                     /locus_tag="ACHURQ_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR0824629.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHURQ_002904"
     CDS             complement(5235..6026)
                     /locus_tag="ACHURQ_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR0824630.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHURQ_002905"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHURQ_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR0824631.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHURQ_002906"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHURQ_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR0824632.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHURQ_002907"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHURQ_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR0824633.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHURQ_002908"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHURQ_002908"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR0824634.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHURQ_002909"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHURQ_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR0824635.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHURQ_002910"
     CDS             complement(9882..10364)
                     /locus_tag="ACHURQ_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR0824636.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHURQ_002911"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHURQ_002911"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR0824637.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZOZY010000128        11295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZOZY010000128 DAZOZY010000000
VERSION     DAZOZY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.128"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHURQ_002912"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHURQ_002912"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR0824638.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHURQ_002913"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHURQ_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR0824639.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHURQ_002914"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHURQ_002914"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR0824640.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..5122
                     /gene="uidB"
                     /locus_tag="ACHURQ_002915"
     CDS             3749..5122
                     /gene="uidB"
                     /locus_tag="ACHURQ_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR0824641.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5161..6471
                     /gene="uidC"
                     /locus_tag="ACHURQ_002916"
     CDS             5161..6471
                     /gene="uidC"
                     /locus_tag="ACHURQ_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR0824642.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6652..8160)
                     /locus_tag="ACHURQ_002917"
     CDS             complement(6652..8160)
                     /locus_tag="ACHURQ_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR0824643.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHURQ_002918"
     CDS             complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHURQ_002918"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR0824644.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9635..11281
                     /gene="fumB"
                     /locus_tag="ACHURQ_002919"
     CDS             9635..11281
                     /gene="fumB"
                     /locus_tag="ACHURQ_002919"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR0824645.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2650 c         2916 g         2917 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt
     5101 aatcagcgat atcactaatt aataatcaat aaaaataatc agaacatcaa aggtgcaact
     5161 atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg ccttatctct
     5221 gcttatgcgg cgcaactggc tgacgatgag gcgggactac gcatcagact gaaaaacgaa
     5281 ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc atgggtacag
     5341 ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt tgaaggcggg
     5401 gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg gtatcttgat
     5461 ggtgataaaa gttttggctt tgccctaggg gcagtaaaaa taaaacccag tgaaaatagc
     5521 ctgcttaaat caggtcgctt cgggacggat tatagttatg gtagcttacc ttatcgtatt
     5581 ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg agcattaggt
     5641 tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg agtattttta
     5701 tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca gacgggaaaa
     5761 tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga taccagtcgc
     5821 tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag tattctcgag
     5881 aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg cttttacgcg
     5941 cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt ggttagtgga
     6001 caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta tttgcgccat
     6061 gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt aagtcttgat
     6121 cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa
     6181 tttacactga catttgcaca gattgtgact cgcggctatg aatccagtaa acgagatgtg
     6241 cggattgaag gcgcgggtat cttaggtggt atgaactatc gggtcagcga agggccgtta
     6301 caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga tggcagtacg
     6361 ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga ctttatgctg
     6421 aagtcagaaa taacgccgga gagaaaaacc tccggcgttt cagattgttg acaaagtgcg
     6481 cgttttttat gccggatgcg gcgtaaacgc cttatccagc ctacaaaaac tcataaattc
     6541 aaagtgttgc aggaaaaggt aggcctgata agcgtagcgc atcaggcaat ctctggtttg
     6601 ttttcagatg aaaacgccga agtgaaaatt ctccggcgtt ttggccgtga attactgctg
     6661 cggaattgcc ggtacagccg gaacgttaag agctggcatc gcaaacatac caacgaaatc
     6721 ttccagtggc attttctgcc cgtttaacgt tatctgaccg ttagtatatt gcaggctggt
     6781 ggtgatggta ttgtcctgca aggtggtcag acggaacatc tgccccattg ctgatgcacc
     6841 ttcaacttgc tgtttcgcca gttttttcgc ttgatcttcc tgataacctt ccagcttcgc
     6901 tacctgagtc ataaactcag ttgccatatc caccggaatg gtcagtttcg catccagaga
     6961 tttaaccgaa cgatctactt cctgcgccag cgtttgcggc gcttctttag tcgttgccgg
     7021 atctttcagg aacagcgaca gattcagcgc actttcaccc tgactgtttt tccagcttag
     7081 cggcgcgata gtaatcaccg gatcgccttt caacatcagc ggcagggcgc taaagaaggc
     7141 ttccgtcact ttctcctgat aaagttcggg attgttggca atttctggct gtgccagcag
     7201 cgcctgagtt tgcgcgttat attgctggct aaactgatgc caggcttcac catcaatctg
     7261 gccgactttt aaagtcagct tgccgctgcc cagatcctga ttctgtacct tcaggctgtt
     7321 tagcgagtaa tccagttggc tattgatcgt tttaccgtca ttgaccagat ccgatttacc
     7381 gctgatctcc atgccttcca gcagtgccag ttctttgcct tccactgaaa tggtcatttt
     7441 ttccagtgac agtttttggt ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc
     7501 gtcggttttc agattattaa aggtcaactg cactttctgg ttgtattcgt taactgcgtc
     7561 tatccgacca ctttgcgcct ccccggaaag ggagatggct ttgccgtctc tgtcagcatt
     7621 taactggaac tcgccgccgc taaaggcgac tttttcatcc ttttgctcgt aattcagtgg
     7681 cttgagcgaa atatcggaac tggaatcacc gctgtaacca atgcgcgagt taatctcaaa
     7741 aggcgtttca ccttttgcca tatcaaacag tggtttgctt acttcgttat taaccagcgt
     7801 ggtttgaatt gatgccatcg acgggatcag gttcagtttt ttaagctggg caagcgggaa
     7861 gggaccatga tcaaccgatt cgttgaagat gacgctctga ccgcttttaa tccacggatt
     7921 ttctttcccg gcaatgggtt tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg
     7981 atagttttga taactcactt ccaggttgga ttcaggagct gtcagtttga gttgcgcgtt
     8041 cgcctgcgcg accatgtctt cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc
     8101 gcctgtccag actacgccta gcgcaacaat gacgcctacc gctaccagcg atttattcat
     8161 aatgattatc cataaaatga aatcaggcgg actggccgcc tgaaggtgtt ataagccttt
     8221 aataagctta gcaagagatg ttaatttttt cagtaagctc ttacagcttg ttgtaaacac
     8281 gcgctaaacg gccgtggcct ttgacagtca ccggtgattc gttggcggca ataaacgctg
     8341 attcacccgg tttaagctgt aactgctgag aacctttcca caacgttgca tcgccttcga
     8401 cgcagaacaa aatggcggca ctctgctggc taatggtggt ttctttatca ctaaggtcat
     8461 gcagcgagaa ggcaaaatca tccactggaa tcgggaagtc cagttctgca ccttgtttca
     8521 ccggctgggt caacaactgg ttagccggtt tggcttcgaa tttcacattg gcaaccagtt
     8581 ccggaatatc aatgtactta ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca
     8641 tcacttccag cgccacgcct tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg
     8701 cttcgccagg attcaatttc accacattca gtaacagcgg ggagaacagg ccactgtctt
     8761 ccgggtaaaa ttcagaaatt aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga
     8821 gcgccgattt taaaatcgcc agcgcgcggg atttttcttc accctgcata ttcaacaggc
     8881 tggcgaacag ttcgcttaaa cgttcggcat taggctgttg taaaaagtga gcaattgccg
     8941 gatgtgcacc tgcgactggc tggagtaggg agacaatctc ggaaaactca cgaaacgcgt
     9001 tcatcgcaag gaaaggcgtc agcgcaaaaa ccagctccgg cttgtggtta ggatctttat
     9061 agttacgctc ggcggcatcc atcgggatac ctgcggcatt ttctttggca aaaccgattt
     9121 cagaattgcg tttgtttgga tgaacctgaa tggagagcgg ctgtgctgcg cataatactt
     9181 tgaacaggaa aggcagttcg ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt
     9241 tatcactctc aatcacatca cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg
     9301 aactgctttt cggatgtgcg cccatccaca gctcggccat cggctggctg gacggatttt
     9361 ccataccata aagttcagtc aacgccgttt tgctgcccca ggcatagttt tgcactgagt
     9421 taatgagttt ttgcatgatc aatccctgtt ttaatgtgga aattaatccc actattaaag
     9481 caagaatcct acgggaagta acctggagcc gcaaaaagtc gtactagtct cagtttttgt
     9541 taaaaaagtg tgtaggatat tgttactcgc ttttaacagg gcaacggaac acccgcccag
     9601 agcataacca aaccaggcag taagtgagag aacaatgtca aacaaaccct ttcattatca
     9661 ggctcctttt ccactcaaaa aagatgatac tgagtattac ctgctaacca gcgaacacgt
     9721 tagcgtatct gaatttgaag ggcaggagat tttgaaagtc gcacccgaag cgttaactct
     9781 gttggcgcgt caggcgtttc atgatgcgtc atttatgctg cgtccggctc accaacaaca
     9841 ggtggccgac attctgcgtg acccggaagc cagcgaaaat gataaatatg tggcgctgca
     9901 attcctgcgc aactccgaca tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg
     9961 cactgcgatt attgttggta aaaaagggca gcgtgtatgg accggtggct gtgatgaagc
    10021 ggcgctggcg cgcggtgtct ataacactta tatcgaagat aatttgcgct actcgcaaaa
    10081 cgcgccgctg gatatgtata aagaggtgaa taccggcacc aatctgccag cgcagatcga
    10141 tctttatgcc gttgatggcg acgagtacaa attcctctgt atcgccaaag gtggtggttc
    10201 ggcaaacaag acgtatctct atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa
    10261 aaattacctg gttgagaaga tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat
    10321 tgcgttcgta attggtggaa cttctgcaga aactaacctt aaaacagtga aactggcttc
    10381 cgctaaatac tatgacgaac tgccaacgga agggaatgag cacggtcagg cattccgcga
    10441 tgtggaactg gaaaaagaat tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt
    10501 tggtggtaaa tacttcgctc acgacatacg cgtgattcgc ctgccacgtc acggcgcatc
    10561 ctgtccggtt ggtatgggtg tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa
    10621 ccgtcagggg atctggatcg aaaaactgga acataatcca ggtaaatata tccctgaaga
    10681 gctgcgtaaa gcgggagaag gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga
    10741 gatcctcgca cagttgtcgc agtatcccgt ttctacacgc ttatcgctta acggcacgat
    10801 tatcgtcggt cgtgatattg ctcacgccaa actgaaagag cggatggata acggtgaagg
    10861 gctgccgcag tacattaaag atcatccgat ttactacgcg ggtccggcca aaacgccgga
    10921 aggttatgcc tccggttctc ttggcccaac gaccgccgga cggatggatt cttatgtcga
    10981 tcaactgcaa gcgcagggcg gaagtatgat catgctggcg aaaggcaacc gcagccagca
    11041 ggtgacggat gcctgtaaaa aacacggcgg cttctacctt ggcagtatcg gtggtccggc
    11101 cgctgtattg gcgcagggaa gtattaagag cctggaatgt gttgaatatc cggaactggg
    11161 aatggaagcc atctggaaaa ttgaagtgga agatttcccg gcgtttatcc ttgtggatga
    11221 taaaggaaat gacttcttcc agcagataca actcacacag tgcactcgct gtgtgaaata
    11281 aacagagccg ccctt
//
LOCUS       DAZOZY010000129        11286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZOZY010000129 DAZOZY010000000
VERSION     DAZOZY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.129"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            423..1037
                     /gene="evgA"
                     /locus_tag="ACHURQ_002920"
     CDS             423..1037
                     /gene="evgA"
                     /locus_tag="ACHURQ_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR0824646.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            1042..4635
                     /gene="evgS"
                     /locus_tag="ACHURQ_002921"
     CDS             1042..4635
                     /gene="evgS"
                     /locus_tag="ACHURQ_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR0824647.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(4691..5836)
                     /gene="yfdE"
                     /locus_tag="ACHURQ_002922"
     CDS             complement(4691..5836)
                     /gene="yfdE"
                     /locus_tag="ACHURQ_002922"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR0824648.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5910..6854)
                     /gene="yfdV"
                     /locus_tag="ACHURQ_002923"
     CDS             complement(5910..6854)
                     /gene="yfdV"
                     /locus_tag="ACHURQ_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR0824649.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6924..8618)
                     /gene="oxc"
                     /locus_tag="ACHURQ_002924"
     CDS             complement(6924..8618)
                     /gene="oxc"
                     /locus_tag="ACHURQ_002924"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR0824650.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8672..9922)
                     /gene="frc"
                     /locus_tag="ACHURQ_002925"
     CDS             complement(8672..9922)
                     /gene="frc"
                     /locus_tag="ACHURQ_002925"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR0824651.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10435..11058)
                     /locus_tag="ACHURQ_002926"
     CDS             complement(10435..11058)
                     /locus_tag="ACHURQ_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR0824652.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3319 a         2529 c         2306 g         3132 t
ORIGIN      
        1 gttccactat tatctctcat ttcgcataga tgaaatttat gaattgaatg aaaagagtat
       61 tcattcttca tatcaaacac agtgcatgtg ttgcacatta tcccaggaac gttggaggcc
      121 taatttaaat gaggtgcaaa aaaaacaaca gaggactaaa ccgtggcttt tgcaatacaa
      181 ttcttacgcc tgtaggatta gtgagaagac ttatagtgct aacttgaaac tataaatcat
      241 cggtataatc cctgatttta ttgttgacat ttcatttatg ccgactattt atatggtata
      301 cttgtcgaat tatcttaaag gaagctcaga ttttcttatt tttattgaga aaatgagatg
      361 acgccttatg tctgtattac tacagggaga agggagatgc ttcattgcaa agggaataat
      421 ctatgaacgc aataattatt gatgaccatc ctcttgctat cgcagcaatt cgtaatttat
      481 tgatcaaaaa cgatattgaa atcttagcgg agttaactga aggcggaagc gccgttcagc
      541 gggtggaaac acttaagcct gatatcgtca tcattgatgt cgatatcccc ggagttaacg
      601 gtatcctggt gttagaaacg ctgaggaagc gccaatatag cggaattatt attatcgtct
      661 ccgctaaaaa tgaccatttt tacgggaaac attgtgctga tgctggcgct aatggtttcg
      721 tgagtaaaaa agaaggcatg aacaatatca ttgcggctat tgaagctgca aaaaatggct
      781 actgctattt ccccttctct ctcaaccggt ttgttggaag tttaacgtcc gaccagcaaa
      841 aactcgactc cttatcgaaa caagaaatta gtgtcatgcg gtatattctt gatggcaagg
      901 ataataatga cattgcggaa aaaatgttca tcagcaacaa aactgtcagc acttataaaa
      961 gtcgcctgat ggaaaaatta gaatgtaaat cactgatgga tctttacaca ttcgcacaac
     1021 gtaacaaaat cggctaacca catgaagttt ttaccctata tttttcttct ctgttgtggt
     1081 ctttggtcga ccataagttt cgcagacgaa gattacatcg aatatcgtgg catcagtagt
     1141 aacaaccgtg tcatacttga tccagtacgt ctgagcaaca aggaattacg ttggttagcg
     1201 agcaaaaaaa atcttgtgat tgcagtacat aagtcccaaa cggctacgtt gttgcatacc
     1261 gattcgcagc aacgagttcg tggtattaat gctgattatt taaatctttt aaaaagaacg
     1321 ttaaatatca aattaacact ccgggaatac gccgatcatc aaaaagcaat ggacgcgctg
     1381 gaagaaggtg aagtcgatat agtgttatca catttagtag cttcgccgcc tcttaatgat
     1441 gacattgctg caaccaaacc actgataatt acctttccgg cgctggtaac cacccttcac
     1501 gattcaatgc gaccgcttac ctcatcaaaa ccagtaaata ttgctcgagt agcaaattat
     1561 ccccctgacg aggtaattca tcaatcattt ccaaaagcaa caattatctc ttttacaaat
     1621 ttatatcagg cattagcatc cgtctcagcc ggacagaatg attactttat tggtagtaac
     1681 atcattacca gcagtatgat ttcccgctat ttcactcact ccttaaatgt agtgaaatat
     1741 tataactcac cgcgtcaata taattttttc ttgaccagaa aagaatctgt cgttcttaat
     1801 gaagtactca atcgatttgt tgatgctttg acaaatgaag ttcgctatga agtatcacaa
     1861 aattggcttg atacaggaaa cctggccttt ctgaacaaac cattagaact cactgaacat
     1921 gaaaaacaat ggattaagca gcatcccgat ttaaaggtgc tggaaaatcc ttactcccct
     1981 ccctattcta tgacggatga aaatggctcg gttcggggcg ttatggggga tattcttaat
     2041 attattactt tgcaaacagg tttaaatttt tctccgatca ccgtttcaca caatatccat
     2101 gctggaacac agctgaatcc cggcggatgg gatatattac ccggtgctat ttatagtgaa
     2161 gatagagaaa ataatgtttt atttgctgaa gccttcataa caacgcctta cgtttttgtc
     2221 atgcaaaaag cgcctgacag tgaacaaaca ttaaaaaaag gaatgaaagt tgccattcca
     2281 tattattatg agcttcattc gcaattaaaa gagatgtatc cggaggtaga gtggataaaa
     2341 gtcgataacg ccagcgctgc atttcacaag gtcaaggaag gcgaacttga tgctctggtc
     2401 gcgacacagt taaattcacg ttacatgatc gaccattact atcctaatga actttatcat
     2461 tttcttattc ccggcgttcc gaatgcatcg ctttcgttcg cttttcctcg cggagaaccg
     2521 gaacttaagg atattattaa taaagcactg aatgcaatcc ccccaagcga agttctgcgc
     2581 ctgaccgaaa aatggattaa aatgcccaat gtgaccattg acacatggga cctctatagc
     2641 gagcaatttt atattgttac gacattatcc gttttattag ttggcagtag ccttttatgg
     2701 ggattctacc tgttacgctc agttcgtcgt cgtaaagtca ttcagggtga tttagaaaac
     2761 caaatatcat tccgaaaagc actctcggat tccttaccga atccaactta tgttgtaaac
     2821 tggcaaggta atgtcattag tcataatagt gcttttgaac attatttcac tgcggattac
     2881 tacaaaaatg caatgttacc attagaaaac agtgactccc cctttaaaga tgttttttct
     2941 aatgcgcatg aagtcacagc agaaacgaaa gaaaatcgaa caatatacac acaggttttt
     3001 gaaattgata atggcatcga gaaaagatgc attaatcact ggcatacatt atgcaatctg
     3061 ccagccagcg atcatgctgt ttatatttgt ggttggcaag atattacaga gacgcgtgat
     3121 ttaattcatg cactcgaagt agagagaaat aaagcgatca atgcaactgt cgcaaaaagt
     3181 cagtttctgg caacaatgag tcacgaaata agaacaccaa taagctccat tatgggcttc
     3241 ctggaacttc tgtcgggttc tggtcttagc aaggagcaac gggtggaggc gatttcactt
     3301 gcctactcca ccggacaatc actcctcggc ttaattggtg aaatccttga tgtcgacaaa
     3361 attgaatcgg gtaactatca acttcaacca caatgggtcg atatccctac tttagtccag
     3421 aacacttgtc actctttcgg tgcgattgct gcaagcaaat cgatcgcatt aatttgcagc
     3481 agtacatttc ctgatcatta cctggttaag atcgaccctc aggcgtttaa gcaggtctta
     3541 tcaaatttac tgagcaatgc tctcaaattt accaccgagg gggcagtaaa aattacgacc
     3601 tccctggttc acattgatga caaccacgct gttatcaaaa tgacgattat ggattctgga
     3661 agtggattat cgcaggaaga acaacaacaa ctctttaaac gctacagcca aacaagtgca
     3721 ggtcgccagc aaacaggatc tggcttaggc ttaatgatct gcaaagaatt aattaaaaat
     3781 atgcagggcg atttgtcatt agaaagtcat ccaggcatag gaacaacatt tacgatcaca
     3841 atcccggtag aaattagcca gcaagtggcg actgtcgagg caaaagcaga acaccccatc
     3901 acactacctg aaaagttgag catattaatc gcggatgatc atccgaccaa caggctatta
     3961 ctcaaacgcc agctaaatct attaggatat gatgttgatg aagccactga tggtgtgcaa
     4021 gcgctacaca aagtcagtat gcaacattat gatctgctta ttactgacgt taatatgccg
     4081 aatatggatg gttttgagtt gactcgcaaa ctccgtgagc aaaattcttc cttacccatc
     4141 tgggggctta cagccaacgc acaggctaac gaacgtgaaa aagggttaag ttgcggcatg
     4201 aacttatgtt tgttcaaacc gttgaccctg gatgtactga aaacacattt aagtcagtta
     4261 caccaggttg cgcatattgc acctcagtat cgccaccttg atatcgaagc cctgaaaaat
     4321 aatacggcga acgatctaca actgatgcag gagattctca tgactttcca gcatgaaacg
     4381 cataaagatc tacccgctgc gtttcaagca ctagaagctg gcgataacag aactttccat
     4441 cagtgtattc atcgcatcca cggtgcggct aacatcctga atttgcaaaa gttgattaat
     4501 attagccatc agttagaaat aacacctgtt tcagatgaca gtaagcctga aattcttcag
     4561 ttgctgaact ctgtaaaaga acacattgca gagctggacc aggagattgc tgttttctgt
     4621 cagaaaaata actaaatagc ggctcccaca atgttctaat gtgggagcta tttaccagca
     4681 catcttacat ttatgatgag aactcctggc gaatttgttc cccatgctgg tcgagcgttg
     4741 ccgctcccgg cataacatgc gggtccgcgc agccgctgat ttttatcgga ttacccggca
     4801 tcattattcc tccggcttca atcaacatat ttctcgcctg agtttgtggc aaattaatgg
     4861 cctcagccac acttaataac ggcgcgacgg gcacaccaac ttcatgtatt ttggctaacc
     4921 aaacttcagc tgcctgcgtt tttaacgttc gctcaatata ttgtttaaga atagcctggt
     4981 tttgtacgcg taaaatattg ctgctaaatc ggggatcatt aaccagttcc gtaagctcca
     5041 gtgcctggca taacgcagaa aaaagcttgt cattaccaca acaaatcgta atcggcttat
     5101 cctgagtatc gaaaacatca aaaggtgcca tgtagggatg gcgatttccc aggcgttgtg
     5161 gtgacttccc tgtcgcaata tatgccatca gaccatgctc cagaaaactc agcgtggcat
     5221 caaacatcgc tatatcgaca tgcgcccctc tctggctctt ttcgcggcca taaagtgcac
     5281 tcactattcc gctgaataaa tagacaccgc cgcatagatc cgcaagagag gtaccaacac
     5341 gcactggcgg agcatcaggg taccctgttt ccatcattat cccactcatt gcctgaatga
     5401 tggtatcgta ggcaggagca tcttttagcg gaccggtatg tccgaaacct gacgatgaag
     5461 catatatgag gcgcgggttg atttcttgta acctttccca tgaaaacccc agtttttcca
     5521 ttgtacctgg gcgaaaattc tcagctaata catcagcttg tttgagcata tttataaata
     5581 tacttttatc gtgatcattc tttaaatcaa gaaccacact ctctttgcca tgattaataa
     5641 aactgtaata gagtgactgt ccatccacat agggaccaaa tgtgcgggta tcatcaccat
     5701 gacctggtgg ctcaacttta attacccttg cgcccatatt acaaagaagt tgagttccga
     5761 aaggtccatt aaggacatgt gtcatatcga taactaataa gccttcaaac ggccctttgc
     5821 tttcattatt tgtcatttgc ttatgccttg aaaaaagccg aaggtgaaaa gacacttcgg
     5881 catttatgta aaatcaataa catgattaat tatgaaacca gtcgactgac ataaatccac
     5941 aagggagccg tgacgacaaa gcccagtacg ctcacagcca atgacgctgt accagtacgg
     6001 gtgtagacat taaaccggct ggcaataatg attccggaga atgccggcgg taatgcgcct
     6061 gccagtacca tcatctgcaa atgttcgctg ttcaaatgac atgccatacc aacgagaagc
     6121 agcgccagtg gcatcagaat caacttcagg aaggtgttat aagcaatttc ggcactgaac
     6181 tcgaatttat gtgcagccag agtcaacccc gcagcgaata ccgctacccc tgagttagcc
     6241 ttcgcaatca gattaaaggt cgggtcccat gctgccggaa tttttacccc aaccagcacc
     6301 aggatcgttg ccagaacagg tgcccatact actggctcct ttgccgcaga aattaatgcg
     6361 ctcagattac tattcttctt accatccgct cctgaggaag gattcagcaa atacagacca
     6421 ataggaatag taattgcgtt aacaataata gaaataattg ctaccactaa acctgttgat
     6481 acggaatcac cataaatagg atcgagaact gcaaacccca ggaatccaat ggtaggtgaa
     6541 cccgcaatta atgcacatac agcggcttct gcatgggtac gtttaaaaaa tttgtagcaa
     6601 ccaaaccagg agaaaaagaa acatccgaca ataaccacaa gtgatacaag ggtcagacga
     6661 gtgtccgcaa aaatcatttc cctgtttgcc cgggtaatag aaacaaatag agccgcagga
     6721 agagcgtagt ttaataccag tttattaaat gcgcgggctt gatcttctga aaaagtttct
     6781 cgtctgccgc taaaataacc caacagcatg attacgataa taggcaataa atcgccaata
     6841 aaaaatgtta gcataatagt atttccgata agggtcccct caccatcgca taatgagttg
     6901 gcaagaggag acgatgtcag tatttaatta ccagcgactt gttttgggtt aagtttggta
     6961 atatggccac tttcagttcc tgcagcaggg tcgatgacca cattaataat ggtcggtttg
     7021 cgcgactgaa taccggtggt taaagcatga cgaagttcat ctgtcgtggt gacgttatag
     7081 ccaacgccac gaaacgcatc cattaatttg tcataccttg catggtgcaa cagatccgtt
     7141 ggtgatggtg caccagcgcc actgagatca acaccgtctc ctctgtagat gccgccatta
     7201 ttaaaaataa cgatcgtcac cggcaggtta tatcgacaaa tcgtttcaat ttccatccca
     7261 ctgaaaccaa aagcactatc accttcaatg gcgacaaccg gagaaccaga agtcacgcta
     7321 gcaccgatgg catagcccat accgatgccc atgacacccc aggtaccaca atccagacga
     7381 cgacgtggtt tatacatatc aataatattt cgtgcattat ccagggtatt tgcaccttca
     7441 ttaactaaat aaatatcctg gttctcgcgc aatacgtcgc gcacagcact taatgcatta
     7501 aagtaattta atggttgagt atctgtactt aatttttcat gcattttttg tgcattttgc
     7561 tgcttgtgga tatttaaaat atcgcgccat accagtggag tcgtaaatgt gttttgtttc
     7621 agttctgcca gcataccttg catactggat gcaatatcac caacgactgg cacagcaatg
     7681 gggcggttgc tgtcaatttc ctgcggttca atatccagtt gaataaactg tgtatctgcc
     7741 gcccatcctt ttttaccgtg tgccaataac caattcagtc gtgcaccaac aagcatgaca
     7801 acgtcagcat ttgccagggc aaacgaacgc gcagctgccg cagaaagtgg atgcgtatct
     7861 tcaaggatcc ctttcgccat agacattggc aggaatggaa tctgagcact ttcaataaat
     7921 tcacgaagct gttcatcagc ttgtgaatac gccgcgcctt tgccaaggat aattaatggc
     7981 cgttcagctt ttgctaaaag cgaaattgcg ctagtgactg acttcgggca tggcaataat
     8041 gctggcgacg gattttcaac tttaacaatc gtggttaacg cttcgtcttt ttccatcgtc
     8101 gcggccagga catttgctgg caaatcaaga taaactccgc cagggcgacc cgatacagag
     8161 acccggatag cgcgtgccaa tgcaatgcca agatcctgcg gctgattaac gcgaaatgct
     8221 gctttggcat acggttttgc cgcattcatt tggtccagct cttcataatc accttgctgt
     8281 aggtcgacga tcgcgcggtc gctggagccg ctaatcatga tcatcggaaa accatttacc
     8341 gttgcgttgg ccaatgcggt caaaccattg aggaatcctg gcgcagaaac tgtcaggcag
     8401 atccccggtt tttgggtaag aaaaccgctt gccgcagcgg catagcctgc cgactgctca
     8461 tgacgaaaac caatataacg aatgccttcc gcctgggcat ggcgtgccat atccgtcaca
     8521 ggaataccta caacaccata aatagtgtca atattattct gttttaatgc ttcaacgatg
     8581 atatgcatac catctgtcat ttgaagttga tctgacatga ttaataacct cgaaattttt
     8641 ataaattggg agccagaagc ccccgttaat atcagatggc gtggttttgc ttcattgcag
     8701 caatttcatc gtcgctataa cccagctcct gcaatacagc agcggtatgt tcacctaata
     8761 gcggcgcagc tttaatatcc ggcgtaaagg cagagaattt cattggacaa ccaacggtca
     8821 gatattttcc acgcaacggt tgttccactt caacaacact gccactttgg cgcaaagagg
     8881 gatcaagtga aatttctttc atacttaaaa ccggtgcaca aggaatatca aactgagtca
     8941 aataggccac cgcttcatgt ttatcaatag tgacagtgta tttttcgatt tcagcaaaaa
     9001 tatcgaaaat atgtggctgt cgggcatggg ctgtactgta tgccgaatcg gtaatccatt
     9061 ctggtttgcc gatggcttta caggtgtttt cccagttttg ctcctgaata gtgaaataaa
     9121 tataggcgtt aggatcggtt tcccagcctt tacatttcag gatccagcca ggctgaccgc
     9181 caccacctgc attaccaccg cggggaactg catcaccaaa tgtaccattc ggatactgcg
     9241 ggtattcttc cagataaccc aatttatcga gacgctgctg gtcacgtaat ttcacgcggc
     9301 aaaggttcaa tacggcatcc tgcattgaca tggtgactcg ttgcccacgc cccgtttttt
     9361 cgcgatgcag caaagcagca agtaaaccga tcagcaaatg cattccggtg ttgctgtcac
     9421 ccaacgctgc agcgcttacc agcggcggac catcccaaaa acccgtagtg gatgccgcgc
     9481 cacccgctgc ctgagcaacg ttttcatagg cttttacatt cacataaggc gaacactcat
     9541 caaacccttt gatcgaacca aaaatcagac gtggattgat ttcttgaata tgctcccagg
     9601 tgaagcccat gtgatcaatg gcccctggat gaaagttctc gactaagata tcagcttcgc
     9661 ggatcagctt ttccattacc tctttgcctt ccgctgtttt ggtatttaac tcaatagaac
     9721 gtttgttact gttaagcatg gtgaagtaaa gcgcatcgat atcaggaata tctcgcagtt
     9781 ggtgacgcgt tacgtcacca acgccgggac gttcaatttt aataacgtca gcgccaaacc
     9841 aggccagcat ttgagtacaa gatgggccag attgcacacc ggtgaaatcg agaactttaa
     9901 ttccttgaag tggagttgac atagcaatac ctattagtta attaaatatg tgttaattta
     9961 gcgtttattt aaggcataac gccctctata aataatctgc aaaatatctt cgtagcggca
    10021 ctcgatattt ttaaattcct gtatcaagta ctgaaaatta tttcatcgcc acttccaatt
    10081 cctttctgca tggtaagtag tgaggacgaa aagaagaata tgataaatat ttattcgtgg
    10141 taatcagaga gagtcgcgac atttgtgatg ttattcttac aactgtaaga agcgccggtg
    10201 atttacaagt aagagtatga ttacgctttt taaagcgtac cataaagcat aacataataa
    10261 atgatttgtg atgacactgg attattgcgg tttactttga ggaaaataaa atgcgtgttt
    10321 atagaaccgc gagcgccaaa taattttatg accacaacct gtcgtattgt ggtcataaaa
    10381 aattgtcggc tggagggagt taaatccttt acctcaatat actgcctgtc agcattagtt
    10441 cacaaacagt gcttcgctat cgacgatgat tccatcttcc gcacctttaa gcgccaggtt
    10501 tgcctcgtag tattgctttt tatcaagcag tttctgcgca tcagcaagtg cattacgagt
    10561 ctgtttaagc ggcatcagat attggttttc cattacgcca acaccggcca ggcgaagctc
    10621 ttccattgcg ccctttttat cccctttcgc cattttttca ttggcaattt ttatcgcggc
    10681 ttctttttcg ggtgttgcta cataactctc agaaattccg acagatgcat taatgacaat
    10741 atattggtca tcattgagat tggtcttttt acctggctta gcgaactttg cccattcggt
    10801 actgtcatct gacagcaaag cggaagcttc attggtcagt tcttttgcct tttccggatc
    10861 gccatgaaac agagccaggc gcgctacctg cacgtcacgc atcgcatata aaccttgttc
    10921 agagatacgc tctgctgctg ccgttttctg ggtttgctga gtttgctgtt gtgccgctac
    10981 cggtgcatta tcagcagccc atacagttga agatgccaga attgctgtga ccatcgtagc
    11041 cataattaaa cgtttcatac ttacctcatt ctttaacagt tttagaaaac gccgccatca
    11101 tattgggtga gcatgaaatg agaaatcaga gagcaacggt cattttgaaa gggttatctt
    11161 acaattgtag ttattatcgc aaagcggcat ttagtcttac agttgtagga atatgcttcc
    11221 tgtcgcagag aactattctt atataccaca aagagatgtc atttattata ttgatgtaaa
    11281 tattga
//
LOCUS       DAZOZY010000130        11277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZOZY010000130 DAZOZY010000000
VERSION     DAZOZY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.130"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHURQ_002927"
     CDS             79..213
                     /locus_tag="ACHURQ_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0824653.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHURQ_002928"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHURQ_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR0824654.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHURQ_002929"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHURQ_002929"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR0824655.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHURQ_002930"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHURQ_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR0824656.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHURQ_002931"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHURQ_002931"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR0824657.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAVLSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHURQ_002932"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHURQ_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR0824658.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHURQ_002933"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHURQ_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0824659.1"
                     /translation="MKLNIRAQTAQNQHNNSPIALVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHURQ_002934"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHURQ_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR0824660.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHURQ_002935"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHURQ_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR0824661.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHURQ_002936"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHURQ_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR0824662.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHURQ_002937"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHURQ_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR0824663.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHURQ_002938"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHURQ_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR0824664.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHURQ_002939"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHURQ_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2756 a         2776 c         2805 g         2940 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 caccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ctcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaat
//
LOCUS       DAZOZY010000131        11218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZOZY010000131 DAZOZY010000000
VERSION     DAZOZY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.131"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHURQ_002940"
     CDS             53..352
                     /locus_tag="ACHURQ_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR0824665.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHURQ_002941"
     CDS             complement(472..651)
                     /locus_tag="ACHURQ_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR0824666.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHURQ_002942"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHURQ_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR0824667.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHURQ_002943"
     CDS             2090..2668
                     /locus_tag="ACHURQ_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR0824668.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHURQ_002944"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHURQ_002944"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR0824669.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHURQ_002945"
     CDS             4347..5945
                     /locus_tag="ACHURQ_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0824670.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVQFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHURQ_002946"
     CDS             complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHURQ_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR0824671.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHURQ_002947"
     CDS             complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHURQ_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR0824672.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7968..8939
                     /gene="manX"
                     /locus_tag="ACHURQ_002948"
     CDS             7968..8939
                     /gene="manX"
                     /locus_tag="ACHURQ_002948"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR0824673.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9002..9802
                     /gene="manY"
                     /locus_tag="ACHURQ_002949"
     CDS             9002..9802
                     /gene="manY"
                     /locus_tag="ACHURQ_002949"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR0824674.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9815..10666
                     /gene="manZ"
                     /locus_tag="ACHURQ_002950"
     CDS             9815..10666
                     /gene="manZ"
                     /locus_tag="ACHURQ_002950"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR0824675.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10721..11179
                     /locus_tag="ACHURQ_002951"
     CDS             10721..11179
                     /locus_tag="ACHURQ_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR0824676.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2757 a         2823 c         2830 g         2808 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca actcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcaatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggc atcgggttat cggcatcgtg ctgaacattg
     6721 gcctgacgtt tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc
     6781 aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct ggttaaacac ttcgatgatg
     6841 atcgagaagc caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct
     6901 gccaccagac tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg
     6961 tgctggttaa cgaatcgcgt cagcggtttg gatgccagca acataaccgc catcgcaatc
     7021 actaccgccg ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca
     7081 tccaacgaga agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg
     7141 tagcctttac cgtggccgga atcatgatcg cggttttcca gccgttcatg cagttcggtt
     7201 gttgctttga acagcaagaa tatccccccg aacaacataa tcaggtcgcg tccggagaag
     7261 gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac
     7321 agcagcccca gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt
     7381 tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc
     7441 acaatttcga gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat
     7501 tccatgacaa gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa
     7561 cgacaaaatg caaacgaaag gtgcggcata gagtgccaga aaggcaggcg ttaaaaggcc
     7621 tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg
     7681 ttgttaaacg ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc
     7741 tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa tttctgctaa tcgaaagtta
     7801 aattacggat cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg
     7861 caggggtttt tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa
     7921 atacatctgg cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta
     7981 ttgttatagg cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt
     8041 taggcgagca ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat gccgaaacgc
     8101 tgattgaaaa gtacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc
     8161 tcgttgatac atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag
     8221 agcattatga agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggctc
     8281 gtgatgatga cccaagcttt gatgaactgg ttgcgctggc agtagaaaca ggccgtgaag
     8341 gcgtgaaagc actgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag
     8401 caccaaaagc ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg
     8461 gccttgcgcg tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag
     8521 aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta
     8581 agacactgct cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca
     8641 aaatgattcg cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta
     8701 ccaacccaac agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg
     8761 tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg
     8821 aaaaagatat tgaggcgttc aagaaactga atgcgcgcgg tattgagctg gaagtccgta
     8881 aggtttccac cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac
     8941 gtattgtgtt gattatcact cagttttcac acttaagtct tacgtaaaca ggagaagtac
     9001 aatggagatt accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat
     9061 gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg
     9121 tatcgttctt ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc
     9181 gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat
     9241 ttctaccatt ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat
     9301 ccctctggcc gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt
     9361 ccagcacgct gcggataagg ctgctgataa cggcaacctg acagcgattt cctggatcca
     9421 cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca tcgttgcgct
     9481 gtctgttggt accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa
     9541 tggtctgaat atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat
     9601 gatgcgtgct ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac
     9661 caactttaac ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca
     9721 acttagcccg aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga
     9781 tctcgataac gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac
     9841 cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta acctcttcca
     9901 gggttcatgg aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc
     9961 aattcgtcgc ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct
    10021 ggagttcttt aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa ccctggcgct
    10081 ggaagaacag cgtgctaatg gcgcagagat cgacgacggt gctatcaacg gtatcaaagt
    10141 cggtttgatg gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc
    10201 ggtatttgca gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct
    10261 gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcgtatgg
    10321 ttactccaaa ggtatcgata tcgttaaaga tatggggggt ggcttcctgc aaaaactgac
    10381 ggaaggggcg tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca
    10441 tgtcaacatc ccgctggttg tctctcgcat tactgaccag acgggcaaag aacacgttac
    10501 tactgtccag actattctgg accagttaat gccaggcctg gtaccactgc tgctgacctt
    10561 tgcttgtatg tggctactgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt
    10621 cgtcatcggt atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact
    10681 accggggcct tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt
    10741 actgattctt ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc
    10801 ccgccgtaac ggccccaccc tgctggcaat tcctttgctc cggcgtggtc gcatcgatag
    10861 cgttatcttc gtcggattga ttgtcattct tatctataac aacgtcacga atcatggtgc
    10921 gttaataacg acatggttat taagcgcact ggctctgatg ggtttttata tattctggat
    10981 ccgcgttccg aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga
    11041 atatagccga atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga
    11101 acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct
    11161 tctcgtttca actcaataag ttatgaattt agccaaagct atgtttagtg tattttta
//
LOCUS       DAZOZY010000132        11054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZOZY010000132 DAZOZY010000000
VERSION     DAZOZY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.132"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..392
                     /locus_tag="ACHURQ_002952"
     CDS             <1..392
                     /locus_tag="ACHURQ_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0824677.1"
                     /translation="PWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSAGNNVKVAA
                     DAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKA
                     IQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            603..926
                     /locus_tag="ACHURQ_002953"
     CDS             603..926
                     /locus_tag="ACHURQ_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR0824678.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            923..1753
                     /gene="amiD"
                     /locus_tag="ACHURQ_002954"
     CDS             923..1753
                     /gene="amiD"
                     /locus_tag="ACHURQ_002954"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR0824679.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1750..2763)
                     /locus_tag="ACHURQ_002955"
     CDS             complement(1750..2763)
                     /locus_tag="ACHURQ_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR0824680.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2862..4292)
                     /locus_tag="ACHURQ_002956"
     CDS             complement(2862..4292)
                     /locus_tag="ACHURQ_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR0824681.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4303..5304)
                     /gene="ltaE"
                     /locus_tag="ACHURQ_002957"
     CDS             complement(4303..5304)
                     /gene="ltaE"
                     /locus_tag="ACHURQ_002957"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR0824682.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5341..7059)
                     /gene="poxB"
                     /locus_tag="ACHURQ_002958"
     CDS             complement(5341..7059)
                     /gene="poxB"
                     /locus_tag="ACHURQ_002958"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR0824683.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7192..8160)
                     /gene="hcr"
                     /locus_tag="ACHURQ_002959"
     CDS             complement(7192..8160)
                     /gene="hcr"
                     /locus_tag="ACHURQ_002959"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR0824684.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8172..9824)
                     /gene="hcp"
                     /locus_tag="ACHURQ_002960"
                     /gene_synonym="priS"
     CDS             complement(8172..9824)
                     /gene="hcp"
                     /locus_tag="ACHURQ_002960"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR0824685.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9968..10867)
                     /gene="lysO"
                     /locus_tag="ACHURQ_002961"
     CDS             complement(9968..10867)
                     /gene="lysO"
                     /locus_tag="ACHURQ_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR0824686.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         2693 a         2909 c         2915 g         2537 t
ORIGIN      
        1 aaccatggtt gccagtaaaa tggacaaaag agttaataaa tgattatttg tatggctgtt
       61 tactatctct gtactctatc tgtgcaaggg atatatataa tatgagcgcc gggaataacg
      121 tgaaggttgc tgcggatgct ttccttgaaa ttattttctc tcttaagaat aaatattgca
      181 ttaaactatt aagcgctcag gaacgggcgt ttatttacga atttgcaaga atgatcttcg
      241 cttatattaa tgacaaaagc attgagatct tattactttc ttgttttgcg gcgtctgacc
      301 aaaaagcaat ccagaggtat atacctcagt cacaagatgg ggaagacttt aggagtcatt
      361 tacaatataa gttgcctctt tccgctcatt aatgttgttg gtgcaacata ataagacagg
      421 taagcggtac cctataatga aaccaatatc gggttatgca acggataatc gtaaagactt
      481 aagtgggtgt ttttgacaca atgagatgat ttcctaaact gatcaaaacc ttcttcccaa
      541 catggcatat cagttcgata atgctttaga gatcgaaggc ttaacaaaca aggagatcga
      601 tcatgcaatt ttctacaacc ccaactctgg aaggccagac catcgttgaa tattgcggtg
      661 tggtgaccgg cgaagcgatt ttaggtgcca atattttccg tgatttcttt gccggtatcc
      721 gcgatatcgt tggcggacgt tccggtgcgt atgaaaaaga actgcgtaaa gcacgggaga
      781 tcgcctttga ggaattaggc tcccaggcgc gggcgctggg ggccgatgcc gtcgtcggta
      841 ttgatatcga ctacgaaacg gtcgggcaaa acggcagtat gctgatggtt agcgtcagcg
      901 gtacggcggt gaaaacgcgt cgatgagaag agtcttctgg ctggtcgctg ccgctctgtt
      961 attggcaggg tgtgcaggcg aaaaaggcat tgtcgagaaa gagggatatc agcttgatac
     1021 ccgacgccag gcgcaggcgg cgtatccgcg cattaaagtg ctggtgatcc actacaccgc
     1081 agatgatttt gatagctcgc tggcgacact gaccgataag caggtcagct cgcattatct
     1141 ggtccctgcg gtaccaccgc gatacaacgg taaaccgcgc atctggcaac tggtgccgga
     1201 acaagaactg gcctggcatg cggggattag cgcctggcgc ggggcaacgc gccttaacga
     1261 cacctctatt ggcattgagc tggaaaaccg tggctggcaa aaatcggccg gagtgaaata
     1321 ttttgccccg tttgaaccga cacagattca ggcgcttatt ccactggcga aagatattat
     1381 tgcccgttat cacatcaagc cggaaaacgt agtggcacat gcggatatcg caccgcagcg
     1441 caaagacgat ccggggccat tatttccctg gcagcaactg gcgcagcagg ggattggtgc
     1501 ctggccggat gcgcagcggg ttaactttta ccttgccggg cgcgcgccgc acacaccggt
     1561 agataccgcg tcattgctgg agcttttggc gcgctacggt tatgacgtta aacctgatat
     1621 gacaccgcgc gagcagcggc gcgtgattat ggcattccag atgcatttcc gcccgacgtt
     1681 atataacggc gaagcggatg cagaaactca ggcgattgcc gaagcattgc tggagaaata
     1741 cgggcaggat tagcgcggca gttttccgtg gtcgcgtagc caggcggcag ttttctcgat
     1801 accttcatct agggtgatga tcggctgata acctaactct tcctgcgcac gcgtaatatc
     1861 cagcgtaaag tcaaaattca acttggagac gccgtagtgg gtcagcggcg gctcttttgc
     1921 tgacttgcgg cctaaacgct ccatgctgcg ggcgatcata tccagcatcg ggtaggggac
     1981 ggaacgaata cgacagtcaa tattcaactc gtcgatcagc ttctgcacga tgctgcgcag
     2041 tgtgcgatgc tcgccgttgg tgatattgta cacacgcccg gaaggtagct tatcgcaggc
     2101 ttcctggctt gccagccaca ttgcgtgcac ggtattttca tagtaggtca tatcgaccag
     2161 cgcactgccg ccatgcggta acagaatact gccgtagtgg tgcatcatat gcgccagacg
     2221 gggaataaag actttatcgt gcggtccgaa cagactttgt gggcgcagaa tagtaaagcg
     2281 cgtttgtgga ttcgcctgcg aaagcatatt gatcacttct tcgctggctg ctttgctgcg
     2341 ggcaaactcg ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc gatggtggtg
     2401 ataatcgaag tacagggagg gggaagagat atgaataaag ttacgcacac cccaggcgac
     2461 agcccattca cccaggcggc gagtggcgcg aacgttagcc agatcgaaag cctgttgtgt
     2521 cccccagggt gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg cgagcatcac
     2581 tttagcttgt gatgaaacca gctcggtcag atccgccgga acaaactctg cgcccatttt
     2641 ttccagcaat ttgcccattg cctcgttgcg accggtcgct cgcacgctga tgcctttctg
     2701 gcataaaaac tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa ccagtacctt
     2761 catatcaatc cactgttgtt gagaaaatga cgtgcgcatt cttccgtgat ttcccccatg
     2821 atgcaatggg aaacatgaaa gaataacgca ggttttgtcg attaatctgt gctttgttct
     2881 gccagtctgg cgatttgttt tgccattccg cgaaaaataa acagatgcgc ggggatcatc
     2941 aataaccagt aaaacagccc cggcataccg tgcggatgcc agaaagcgcg gacatcgata
     3001 gtacgatagt cgcctttatc ttccaggcta aaacacagtc gtcccagccc cgacgctttc
     3061 atgccaaata acaacgtaag ttgtttttcc ggttcaacga caatcacttt ccagctatcc
     3121 accgcatcgc cagtctgtaa atattcgcgc tccgggcggc ctttcgccag cttatgaccg
     3181 atcgcgcggt ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa ataacgctct
     3241 ttaccgccga tttggttcac tacctgccat aaagcagcaa ggctggcgga cgttttaacg
     3301 gtaaaccccg cctgtttggc aaaataaccg tactccggtc gccagcgggc aaaggcctga
     3361 gcgtcgtagc cccagtcgct ggagttgacc agtttttcct cctctttcaa cgtgctacgt
     3421 accgcgtcat cgaaagcgat cagccgttgt gggatgagtg cacgtagcgc ggtatcatcc
     3481 gccagcagat cgtgtttcag cccctgaatc aacgccctgg cggtggtggg cggtacggaa
     3541 gtaatcacat tgagaaacca caccgaaatc cagcgggtgg ggagggggat ggggatcaac
     3601 cagcggcgct taccgctcac cgccataaaa tgttcaaact gttgctgata actgagcacc
     3661 tctggtccgg cggcttcgaa gatgcggtgt tcgctggccg gatgatctaa caacgccacc
     3721 agatagtgca gcaagttttc cagcgcgatg ggcgtggtgc gtgaacgtac ccagcgtggc
     3781 ggcgttaaca ctggcaggtt gtagaccata tcgcgcatga cttcgaacgc cgctgaacct
     3841 gcgccaacga taattccggc ccgaagttcg gtcacaggta cattcgcttc acgaagaatg
     3901 tccgccgtag cctgacgagc acgcagatga tccgactgct catgtggcgg ggcctgcaac
     3961 gaactgagaa agattaattg cttaactggt acttcacgta gcgcatcgcg gacgttgaga
     4021 gccacctggc gctcctgagc gataaaatcg ccgccttcgc ccatgctgtg caccagaaaa
     4081 tagacggtat cgatatcctg caacagggcc ggaaggttat ccggccagct gagatcgact
     4141 ttatggcaac tgacgtttgc cagttgcagc tttgcaagcc tgtcgacatg acgtgccgcc
     4201 gccaggatct gatgcccttg ctggctgagt gtgcgcacca gatgctgacc aatgtagcca
     4261 ctggcaccga gaactaaaat gcgttgcggc acgtctctct ccttaacgcg ccaggaatgc
     4321 gcgccagtgg gcggcgactt ccgccagttg ttcgcgcgag acgtcaagat gcgtcaccag
     4381 gcggacaatc ggcgaggcgt taatcagcac gtttctcgct ttcatgtatt cgcctaacgc
     4441 ggcagcattt tcttccccga cgcgaacaaa cagcatatta gtgtcctgac gcatcacatc
     4501 cgcgcctgct tcacgcagtt gctccgccat ccaggcggcg ttgtcgtgat cttcctgcaa
     4561 ccgtgcgacg ttatttttca gggcatacat cccggcggca gccagaatcc cggactgacg
     4621 catcccgcca ccggccattt tccgccagcg gatggcacgt ttaatgtaat cacgattacc
     4681 gacgagtaat gaaccgactg gcgtcccaag acctttcgac aggcaaatgg tgaacgaatc
     4741 acaatattgc gtgatctctt tcagttcgca gccgtaagcc accacggcat taaagatgcg
     4801 cgcaccgtca acatgcagcg ccagattgcg cttgcgggta aattcccatg cttctttcag
     4861 gtattcacgc ggcagcactt tgccgttgtg ggtgttttct agactgagta atttggtgcg
     4921 ggcgaaatgg atatcgtcgg gtttgatttt catcgccact ttatccagcg gtagcgtgcc
     4981 gtcggcagcc gcgtcgatgg gttgcggttg aatactgccc agcaccgctg cgccaccggc
     5041 ttcaaacaga taattatgcg cggcctgacc gacaatatac tcttcgccgc gttcgcagtg
     5101 actgagcaga gcgaccaggt tagcctgggt gccggtcggc agaaaaatgg cggcttcttt
     5161 accggaaagc tctgcggcgt agtcctgcag agcattaacg gtagggtcgt ctccgtaaac
     5221 gtcgtcccca accggggcgg ccatcattgc ttcgagcatg gcgcggctcg gtcgggtaac
     5281 ggtatcactg cgtaaatcaa tcatggcatg tccttattat gacgggaaat gccacccttt
     5341 ttaccttagc cagttcgttt tcgccagttc gatcacttca tcgccgcgtc cgctgatgat
     5401 tgcgcgcaac atatacaagc taaatccttt ggcctgttcg agtttgatct gcggtggaat
     5461 ggctaactct tctttggcga ccaccacatc caccagcacc ggaccgtcga tggagaaggc
     5521 gcgttgcagg gcttcatcaa cttcagacgc tttttctaca cggatacccg taatgccgca
     5581 cgcttcggca atgcgggcaa agtttgtgtc gtgtagttcg gtgccgtcag tcaaatagcc
     5641 accagctttc atctccatcg ccacaaagcc cagcacgctg ttgttaaaga cgacaatttt
     5701 cactggcagt ttcatctgca ctactgagag gaaatcgccc atcaacatgc taaaaccgcc
     5761 atcgccgcac atggcgacca cctgacgttc tggctctgtc gcctgcgcac ccagcgcctg
     5821 cggcatggcg ttagccatcg aaccgtggtt aaacgaacct aacaggcgac gcttgccgtt
     5881 catttttaga taacgtgccg cccacaccgt tggcgtacca acgtcacagg tgaaaatagc
     5941 gtcatcggcg gcaaaatgac taatttgctg cgccagatat tgcgggtgaa tggctttctc
     6001 gctcggttta gctaaatcgt ccagcccttt gcgggcgtcg cggtaatctt ccagcgcttt
     6061 atccagaaac ttgcgatcgg ctttttcttc caccaatgga agcaatgcac gcagagtcga
     6121 cttgatatcg ccgaccagtg ccatatccac cttgctgtga gcgccgatgc tggctgggtt
     6181 gatatcaatc tgaatgattt tggcatcggt cgggtagaag gcgcggtagg gaaattgcgt
     6241 gccgagtagc actaacgtgt cggcgttcat catggtatgg aaacctgacg agaagccgat
     6301 taacccggtc attccaacat catacggatt atcgtattcg acatgttctt taccgcgcag
     6361 ggcatgaaca ataggcgctt taattttccc ggcaaactca actaactctt tatgcgcccc
     6421 cgcgcagccg ctgccacaca tcagggcgat attgctggaa taacgcagca gttgcgccag
     6481 tttgcgtaac tcttcttctt ccggcgtcac gactggttgt ggcgcatgat accagtgcgt
     6541 ggttgcccct tctggcgcag gttttaacgc cacgtcgcct ggtaacacga caaccgaaac
     6601 gccacggtta agcaccgctt tgcgcatggc aatcgccagt acttgtggga tctgctccgg
     6661 gctggaaacc agctcgcaat agtgactaca ttcgcggaat agctcttgtg ggtgggtttc
     6721 ctggaaatag ccgctgccaa tttcgctgga gggaatatga gcggcaatcg ccagtaccgg
     6781 aacgtgattg cggtggcaat cgaacaggcc gttgattaag tgcaggttgc cggggccgca
     6841 cgatccggcg cagaccgcca gttctccgct aagttgtgct tcagcgccag cggcaaaggc
     6901 cgccacttct tcgtggcggg tggacatcca ctcgatggtg cccatgcgat taagactgtc
     6961 actaagaccg ttcagagagt cgcctgtgac tccccagatg cgtttcaccc ctgccgattc
     7021 gagtgttttg gcgatataag ctgcaaccgt ttgtttcatg gttctccatc tcctgaatgt
     7081 gataacggta acaagtttag ttcatctgac ggagggggaa gggatgggag agaaaggagg
     7141 cactaacggt taaatagccc gatgaaagga atatcatcgg gcataaggcg attatgcgag
     7201 aaccaaatcc ccctgcggat ggcaggagca ggccagtacg taaccttcag cgatttcggc
     7261 gtcggtcagc gtcattgtac tgctcaccgt atattcaccg gaaaccactt ttgtcttaca
     7321 gcagccgcaa acacccgcac ggcaggcagc gacaaccgga acgttattgc tttccagcgc
     7381 ctccagtagc gtggtgccaa ccggggcgta aaattctcgt gccggttgca gtttggtgaa
     7441 tttcagaccg ctggtcgccg cttctgctac tggggtgaag aatttctctt taaagaaacg
     7501 cgtcacgccg agcgctttca cttcctgctc tacccaatcc atatacggag ccgggccgca
     7561 ggtcatcacg gtacgtgaag ctaagtcagg tacacctgcc agcagttcgc gagtgagacg
     7621 accagcgata aagccttcgg taacgttatt ttctgccacc agcgttaccg gatagttacg
     7681 ccactcatcg gcgaaaataa catcctgcgg cgtacgcacg ttgtagatca cccgcacatc
     7741 ggcctgtgga cggttcttcg caagccagcg acgcatcgac ataatcggcg tgacgccgca
     7801 gcctgccgcc agcaacagga atttatcttc tgctttatcg tcgcaggtaa attcccccat
     7861 cgcgtccgaa agccagagat aatcaccgcg ttttacatcg cgcgtcagcc actgggagcc
     7921 gacaccgtca tcaatccgcc gcacggtcag ggtgatatat tcactcacgc ctggcgtgga
     7981 ggaaatggtg taagcacgca gcgtttccgc tgagtttcgc acgctgacca gtgcatattg
     8041 cccggcgcga tatgggtagt aatcgtggca aatcagggaa atcgtccaca catccggcgt
     8101 ttcttgcgta atgtgatgaa cctgcatccg ccacgggcat tgattcgttg gcatcgtcat
     8161 cgacaaactc cttacgcgct caacagttgc ttcatgtctt cttcaacagt ggtgatagaa
     8221 cgcaggccga atttctcgtt cagcaccgcc agcaggtctg gtgtcaggaa accaggtgca
     8281 gtcgggccgg tgacgatatt tttcacgccc agagaaagca gcgtcagcag aatgacgatc
     8341 gctttctgtt caaaccagga gagcaccagc gacagcggca gatcgttcac accacagccc
     8401 agtttctctg ccagagtgac agccagaata atcgctgagt aagcatcgtt acattgacct
     8461 gcatctacca gacgcggcag accttcgata tcgccaaact caagtttgtt aaagcgatat
     8521 ttaccacagg cgagggtcag gatcaggcag tcatccggca cgctggtggc gaaatcggtg
     8581 aagtagtggc gctcgccgcg tgcgccgtca cagccaccaa gcaggaagat atgacgcagt
     8641 ttttcacggc tcaccagatc aatcagcgta tcagcagcgc caagcagcgt ctggcgacca
     8701 aaacccacgg tgataaggtg cggaatttcg ctgtacggga agcctgccat ctgttgcgcc
     8761 tgggtgataa ccgcagagaa atcatcaccg tccagatgac gcacgccagg ccagccaaca
     8821 atgctgcggg tccagatacg atcgtcataa gcgcctacgg ttgggtcgat gatgcagttc
     8881 gaggtcatca cgatggggcc agggaaacga gcgaactcca cttgctgatt ctgccagccg
     8941 ctgccgtagt taccgaccag atgcttgaat ttacgcagct ccgggtagcc atgcgcaggc
     9001 agcatttcgc cgtgggtgta gacattaacg cccgtgcctt cggtctgttc cagcaggttg
     9061 tagagatctt tgagatcgtg accggaaatc agaatgcatt tacccgccgt cgctttgacg
     9121 ttgacctggg ttggcgtcgg gtgaccgtat ttaccggttt cgcctgcatc cagaatgctc
     9181 atcactttga agttcatctg gccgatttcc attgaacact caagaagcgc gttcatatcg
     9241 gcaggccagg tccccagcca cgccatgatt ttatggtact gggcataaat atcgttgtcg
     9301 tattgaccga gaacatgcgc gtgttccata taggccgccg cacctttcag gccatacagg
     9361 cacagcagac gcaggccgag aatgttttcg ccaatcgccg ctttatcttt gttaggggta
     9421 aattctgctg cctgacgttg cagctcgccg agatcatcgc tcaccagttg caggtcagcc
     9481 atcgggttat cgacgcgcgc gttggcatct acagccaggc attgtgcttt cagcgcctcg
     9541 cgcaaggcaa tcgcttcacg agcgtagccg acaatacgcg gagaatcgaa gttaacgttg
     9601 gtcagggttg agaaaaaggc acgtggcgcg aagctgtcaa catcgtggtt gatgatgccg
     9661 tattcacacg cttttaccgc ccaggcagaa agcccttgca gcgccgcgat gagtaaatcc
     9721 tgaaggtcag aagtttccgc cgttttacca cacatcccct gcgcgtatga gcagccgttt
     9781 cctgccggag tacggatagt ttgttcacat tgcacacaaa acatgatcac accttttaaa
     9841 gttatattta atatacatgt ttaaggttaa gacgcttaac gcggggataa aagggatttt
     9901 tcatgcaact ttaagggaga ttgatttagc gcaattttgg cggcagggat ctaccgccag
     9961 agaggtatta cgcagagaaa aaggcgatga ggatcggcac taacaggcta agaataaaac
    10021 cgtgaacaat tgccgccggg accatatcca gcccgccagt acgttgaaga acgggcaggg
    10081 tgaaatccat tgatgtggca ccgcataagc ccagtgcagt agagcggctg cggcgaatca
    10141 gcccagggat caacataata gcaatcagtt cacgggccag atcattaaaa aacgccgcgc
    10201 tcccgattac cggaccaaaa gattcggtca ataaaatacc ggaaagagaa taccagccga
    10261 aaccggaggc cattgccagc gcggtattga tggggagatc aagaataaag gcgttaatta
    10321 aaccaccaat taatgaactg acaaccacca ccacggcgac aatcattccc cggcgattaa
    10381 ggacaatctg ctttaaggtc atgccattat tgcgcaactg aataccaacg aggaaaagta
    10441 gcaaaattaa cgtgtattca ctggcttcgg tcgcgtgttg taagaaagcc agtccactca
    10501 gaccaatggc aaaaccaatc actactacgc cgcacagttt tagcgactcc agcgccatcg
    10561 caatacgcga cgggagtttt tcttgctgat ggtggttgcg ccacggcagg cctcgctcca
    10621 gccacatcag ggcggcaata ttacacagta aaataacggt aatactgacg gcagaataat
    10681 gcagaatcgc caacaggtta ctggcgaggt tatcgagaaa cgccagactg atacccataa
    10741 aaaagagaat aaggtaaacc atccagctta atagctgatt aataactttt aacgcagctt
    10801 gttggcgaag cggaatgagg taacctacaa tcaggggaac cagaatgatt aacagcccag
    10861 aaaacatgaa aacccagtcc ttgcaaagat gaagtcgaaa tgcgcgatga cacactactg
    10921 aaagcggaag gacgagtaaa gttgcaatta aaaggaaatg ttatgcataa ggagcagtag
    10981 agtattcgtt ttcatttaaa gatattcttg cgctttaatt acaaactgca ccgatgttgg
    11041 tggcgtcaaa atcg
//
LOCUS       DAZOZY010000133        10967 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZOZY010000133 DAZOZY010000000
VERSION     DAZOZY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.133"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHURQ_002962"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="ACHURQ_002962"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR0824687.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHURQ_002963"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="ACHURQ_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR0824688.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNVQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3743..4162)
                     /locus_tag="ACHURQ_002964"
     CDS             complement(3743..4162)
                     /locus_tag="ACHURQ_002964"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR0824689.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHURQ_002965"
     CDS             complement(4183..5565)
                     /gene="atpD"
                     /locus_tag="ACHURQ_002965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR0824690.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHURQ_002966"
     CDS             complement(5592..6455)
                     /gene="atpG"
                     /locus_tag="ACHURQ_002966"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR0824691.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHURQ_002967"
     CDS             complement(6506..8047)
                     /gene="atpA"
                     /locus_tag="ACHURQ_002967"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR0824692.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHURQ_002968"
     CDS             complement(8060..8593)
                     /gene="atpH"
                     /locus_tag="ACHURQ_002968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR0824693.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHURQ_002969"
     CDS             complement(8608..9078)
                     /gene="atpF"
                     /locus_tag="ACHURQ_002969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR0824694.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHURQ_002970"
     CDS             complement(9140..9379)
                     /gene="atpE"
                     /locus_tag="ACHURQ_002970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR0824695.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHURQ_002971"
     CDS             complement(9426..10241)
                     /gene="atpB"
                     /locus_tag="ACHURQ_002971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR0824696.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHURQ_002972"
     CDS             complement(10250..10630)
                     /gene="atpI"
                     /locus_tag="ACHURQ_002972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR0824697.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2609 a         2997 c         2723 g         2638 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaggtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca attggccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc agcaattcgt tgttcggtgc
      301 aacgacgata accggcatat cggcatcaat aagcgccagc ggaccgtgtt tcagttcacc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccggtaagg gtgtttttta tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtgattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcaac agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaagccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt ttcagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccacaa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatcca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccggcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgcccg actttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa cgaagttacc aacgtgtgca ccttccagca actcagcacc aggacgcaaa
     2401 cgagcaaacg ggccaatagt acaggccgct gccagattcg cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgaaca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttagc gatcagaatg
     2881 ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaagtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaagc cagcctgttt ccagactggc
     3721 ttttgtgctt ttcaagccgg tgttacatcg cttttttggt caactcgata acgcgcagct
     3781 gcgcgatcgc tttggccagt tccgcagacg cctgagcgta atctacgtcg ccgtgagagc
     3841 tgctaatgtg ctcttcagcc ttacgtttcg cttccatggc tcgcgcttcg tcgagatcct
     3901 ggccgcgaat tgcggtgtcg gccagaacgg tcacgttgcc aggctgcact tcaagaatgc
     3961 cgccagacag atagataaac tcttcgtgac cgtgctgttt cacgatgcga atcataccag
     4021 gcttaatggc ggtgagcagc ggtgcgtggc cagggtagat ccccagttca ccttcgctac
     4081 ccgttacctg gattttctcg accagaccag agaacatttg ttgctctgcg ctgacgacgt
     4141 ccaggtggta agtcattgcc atatcaccct ccgattaagg cgttaaagtt ttttggcttt
     4201 ttccacagct tcttcgatgg aaccgaccat gtagaacgcc tgctccggca ggtgatcgta
     4261 ttcgccttcc atgatgcctt taaagccacg gatggtgtct ttcagggaga cgtatttacc
     4321 cggagaaccg gtgaatactt ctgccacgaa gaacggctgg gacaggaagc gctggatctt
     4381 acgagcacgc gctaccacca gtttgtcttc ttcagacagt tcatccatac ccaagatggc
     4441 gatgatgtct ttcagttcct gataacgttg caggatggac tgaacgccac gcgcggtgtc
     4501 gtagtgttcc tgaccaacca ccagcgggtc cagctgacgg ctggtggagt ccagcgggtc
     4561 aacggccggg tagataccca gagacgcgat ctgacggctc agtaccacgg ttgcgtcaag
     4621 gtgcgcaaag gtggttgccg gagacgggtc agtcaagtca tccgcaggta cgtataccgc
     4681 ctgtacggag gtgatagaac cagttttggt ggaggtgata cgttcctgca gaacgcccat
     4741 ctcttccgcc agggtcggct gataacctac cgctgaaggc atacggccca gcagtgcgga
     4801 tacttccgta ccggccaggg tgtaacgata gatgttgtca acgaacagca gaacgtcacg
     4861 accttcgtca cggaatttct cagccatggt cagaccggtc agagcaacgc gcagacggtt
     4921 tcccggcggc tcgttcatct ggccatacac cagggatact ttgtcgataa cgttggagtc
     4981 ggtcatttcg tggtagaagt cgttaccctc acgagtacgt tcacctacgc ccgcaaacac
     5041 agagtaaccg gagtgctcga tcgcgatgtt acgaatgagc tccatcatgt ttacggtttt
     5101 acctacaccc gcaccaccga acagaccaac tttaccgccc ttagcgaacg gacacatcag
     5161 gtcgataact ttgataccgg tttccagcag ttcctgagag tttgacagct cttcgtagga
     5221 aggtgctgcg cggtgaatcg cccaacgctc ttcttcaccg atctcgcctt tcatgtcgac
     5281 cggttcaccc agtacgttca tgatacggcc cagagtcgct ttacctaccg ggacttcgat
     5341 cgggtgttcg aggtctttta catccagacc gcgacgcaga ccgtcggagg aacccattgc
     5401 gatggtacgc acgataccgc cgccgagctg ctgctgaact tccagcacca gacgctcatt
     5461 accattttgc acctcaagag catcgtacac gcgcggtacg gcatcctgag ggaattcgac
     5521 gtcaactacg gcgccgatta cctggacaat ctttccagta gccatcttaa atcctctacg
     5581 aaataacctg tttaaaccgc ggcggccccc gagacgatct cggtgagttc ctgagtaatg
     5641 ctggcctgac gagctttgtt gtataccaac tgcagctctt taatcaggct gccgccattg
     5701 tcggtcgcgg ctttcatcgc caccatacgg gcggcctgct cgctggccag gttttcaacc
     5761 acgccctgat aaacctgaga ttcgacataa cgacgcagca gggtatccag caacgccttc
     5821 ggatcgggtt cgtacaggta atcccaggat ttatgtttca gatcatcatc atctgatgcc
     5881 ggtaacggca gcagctggct gatggtcgga acctgagaca tggtgttaat aaatttgttg
     5941 ctgacaatgt aaagcttgtc cagacggcct tcgtcgtaag cctgcaacat cacttttacc
     6001 ggaccgatca gttcggacag ggaagggtta tcccccatgc cggtgacctg ggcaacaaca
     6061 ttgccgccca cggagttgaa gaacgacacg cctttcgagc cgatcattgc gaggtcgcat
     6121 tgaacgcctt tgtcggtcca ggtcttcatt tccgccagta gttttttgaa caggttaatg
     6181 ttcaaaccac cgcacaaacc acggtcggtc gacaccacca ggtagcccac gcgtttaacg
     6241 tcgcggtctt ccaggtaagg gtgcttatat tccagattac cgtgtgcaag gtgaccaatc
     6301 actttgcgca tggtttctgc ataaggacgg ctggccgcca tgcgatcctg cgatttacgc
     6361 attttggaag cggcgaccat ctccatcgct ttagtgatct tttgcgtgtt ctggacgctt
     6421 gcgatcttac tacgtatctc ttttgcgccg gccatgagct tctcctcaat gccttgcggc
     6481 ctgccctaag gcaagccgcc agacgttacc aggattgggt tgctttgaag gaatcgagga
     6541 tgcctttcag cttgccttcg atttcgtcgt tgtagccacc ggtctggttg atctcttgca
     6601 tcaacggagc gtgatcacgg tcgacgtaag ccagcagagc ggcttcgaag ctgccaattt
     6661 tcgacagttc aacatccgcc aggtaaccac gttctgctgc gaacagaacc agagactgct
     6721 gcgcaacgga catcggcgca tactgtttct gtttcagcag ttcggtcact ttctgaccgt
     6781 ggtcaagctg cttacgtgtt gcatcgtcaa ggtcggatgc aaactgagag aacgctgcca
     6841 gttcacgata ctgtgccaga gcggtacgga taccaccgga cagttttttc atgatcttgg
     6901 tctgtgctgc accaccaaca cgggatacgg aaatacccgg gttaaccgca ggacgaatac
     6961 cggcgttgaa caggttggtt tccaggaaga tctgaccatc ggtaatggag attacgttgg
     7021 tcggaacgaa cgcagaaacg tcacccgcct gagtttcgat aatcggcagt gcggtcagag
     7081 aaccggtttt ccctttcact tcacctttgg tgaaggcttc aacgtattcg gcgttaacac
     7141 gtgcagcacg ctccagcaga cgagagtgga ggtagaaaac gtcgcccggg aatgcttcac
     7201 gtcctggcgg acgacggagc agcagggaga tctgacggta agcaacagcc tgtttagaca
     7261 ggtcatcgta aatgatcagc gcatcttcac cgcggtcacg gaagtattcg cccattgcgc
     7321 aaccggcata cggtgccagg tattgcagtg cagcggattc agacgcggtt gctaccacaa
     7381 cgatggtgtt agccagtgcg ccgtgctctt ccagtttacg taccacgtta gaaatggtgg
     7441 acgctttctg gccgatagcg acatagatac atttgatacc ggaatcgcgc tggttgatga
     7501 tggcatcgat agccagtgcg gttttacctg tctgacggtc accgatgatc aattcacgct
     7561 gaccacgacc gattgggatc atggagtcaa cggctttata accggtctgt accggctgat
     7621 ctacggactg acgttcgata acgcccggag cgattgcttc tacagcagag aagccgtcgt
     7681 gatccagcgg acctttaccg tcgattggtg cacccagagt gttaaccaca cggcccagca
     7741 ggccacggcc aaccggaact tccaggatac ggccagtaca cttaactttc atgccttcgg
     7801 caaggtcagc gtacggaccc ataacaaccg cacctacaga gtcgcgctca aggttcagtg
     7861 cgatagcgta acggttaccc ggcagggaga tcatttcacc ctgcatacaa tcggccaggc
     7921 cgtgaatgcg gataacaccg tcacttacag aaacaatagt accttcgttg tgagcttcac
     7981 tcacaacatt gaactgagca atgcgctgct tgatcagttc gctgatttcg gtggaattca
     8041 gttgcatgct ccagtcccct taagactgca agacgtctgc aaggcgctca agacgaccgc
     8101 gtacgctgcc atcaatgacc atatcacccg ctcggatgat aacgcctgcc attacagact
     8161 tatcgatttt gcaattcagc ttaactttgc gtgacagacg tttttccatc gcagcagaaa
     8221 ttttcgcgag ctgttgttca ctcagtgcgg cagcggaaat gacgtctacc tcagcggtag
     8281 cctcactcac ggcacgaagg tgaataaact gctccagaac atccgggagc gcgttaagac
     8341 gaccattttc agccattacc cgaatcaggt tctgaccgtt ttcgtccagt tgctcaccac
     8401 aaactgcgat aaacgactcg gcgagcgttt ctggcgcaag cgcgccagag agaagctctg
     8461 ccatttgttc gtttttggtt acctcggcgg caaacgccag catgtcctgc cagcgttcta
     8521 cactttggtg ttcgacggca aagtcaaaag ctgctttggc gtaggggcga gctaccgtaa
     8581 taaattcaga catcagcccc tccctcctta cagttcagcg acaagtttat ccacgatgtc
     8641 gctgttagca gcttcatcca cggaacgttc gatgatcttc tcggcgccag caacagccag
     8701 gatagcaact tgcttacgca gctcttcacg ggcacgttta cgctcggctt caatttctgc
     8761 ctgcgcctgg gccacgattt tagtacgttc ctgttctgcc tcagctttcg cttcgtccag
     8821 aatctgcgag cggcgtttgt tcgcctgctc gatgattacc tgggcttccg ctttcgcttt
     8881 tttcagctgg tcggtcgcgc tggcctttgc aaggtcaagg tccttatgtg ctcgttctgc
     8941 ggaagcaagg ccgtcagcaa tttctttttg acgtttttcg atggctgcca ttaatggcgg
     9001 ccatacgtac ttcatgcaga acagaacgaa caggacaaac gcgatggcct ggccgaggat
     9061 tgttgcgtta agattcacag cacaatgcct ctatttagtt aacgttctga tattgctctt
     9121 taaataaaag caacgcttac tacgcgacag cgaacatcac gtacagaccc agacctacag
     9181 cgatcatcgg gatagcatcc accagaccca taacgataaa gaactgagta cgcagcagag
     9241 gaatcagatc aggttgacgc gctgcgcctt ccaggaattt acccccgagg atgccgatac
     9301 cgatcgcagc accgattgcc gccagaccca tcatcacagc ggcagccatg tacagcagat
     9361 ccatattcag gttttccatg acagtctcca gtttgtttca gttaaaacgt agtagtgttg
     9421 gtaaattaat gttcttcaga cgccatcgac agatagacga tcgtcagaac catgaagatg
     9481 aaggcttgca gcgtaatgat caggatgtgg aaaatggccc acggcacatt caggatccac
     9541 tgtgaccacc acggcaacag accagcaatc agaatgaaaa tcagctcacc ggcatacatg
     9601 ttaccgaaca gtcgcaaacc gagtgaaact ggtttggaca gcaggcttac cccttcaagg
     9661 attaagttga caggaatgaa cgcccagtga ttgaacggct gcagcgtcaa ctctttcgtg
     9721 aagccgccga tgcctttcat tttgatgctg tagaacagaa tcaggataaa tacgcccagt
     9781 gccatagaca gcgttacgtt cacgtccgca gacggaacca cacgcagtgc aggcagaccc
     9841 agtacatgtt cagcaatgta cggcagcagg tcgataggca gtaaatccat caggttcatc
     9901 aggaataccc agacgaagat cgtcagggcc agcggagcaa tcagcttgct tttgccatgg
     9961 tacatgtctt tcacgctacc attaacaaag ccgatcacca gctcaatcgc ggtctgaaac
    10021 ttacctggca caccgctggt cgcctttttg gctacgctac ggaataaaac caggaacaac
    10081 agacccagca ccaccgagaa gaacatggag tcaatattga ttgtccagaa ggtggctggg
    10141 gggttttgtg gatccaccag cgagaatgta cgcaggtcca gctgaaggtt attcaggtgg
    10201 tgtcctatgt aatcctgcgg cgtcatattt tctgaagcca tgatgccttt taccctttgt
    10261 tgttaattac agccggtgcc agtatctgaa ccaccagcac caaaacccac gtaacgatca
    10321 gcggcaagaa taccgccttt aaaaccgcca acgccaccac cagtaacacc aacatcgcca
    10381 gaactttgaa agcttcgcca aatgcgaatg tccaggccac ccggcctttc gctggtgtat
    10441 acgcctggtg acgccaggca aatatcataa acaaaacgtt aggcagaaag actgccaggc
    10501 ccccgcttat tgcagagacg ccccagaagg ggtctttgag gctgaacagc aatccacttg
    10561 ctatcaccac cagtaactga acgagcagaa gcttccgagc aacgtttcga ctcacgagcg
    10621 acacagacat cacgtttttc actcctgctc ccttcgaggt atgccgcgtg tcgtataaaa
    10681 ctttctttaa ggcttagagt caagcatcaa aaagcggtca aattatacgg tgcgcccccg
    10741 tgatttcaaa caataagtag ccaaaaggtg aataaatgtt taaatatttt tccagtgcat
    10801 acaattgcga cttttccgct aaccctgttc gatcatgaaa aactgtaaat aacgcgtaaa
    10861 cactagtgat aaagcgtgct tcagatcaca tattgcgcat gttcgcgcac agtatattta
    10921 tttacttggc aaatgatgcc tttgcaagtt tatgatattt cagtcta
//
LOCUS       DAZOZY010000134        10939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZOZY010000134 DAZOZY010000000
VERSION     DAZOZY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.134"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            108..605
                     /gene="ftnA"
                     /locus_tag="ACHURQ_002973"
     CDS             108..605
                     /gene="ftnA"
                     /locus_tag="ACHURQ_002973"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR0824698.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(643..882)
                     /locus_tag="ACHURQ_002974"
     CDS             complement(643..882)
                     /locus_tag="ACHURQ_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR0824699.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHURQ_002975"
     CDS             1073..2284
                     /gene="tyrP"
                     /locus_tag="ACHURQ_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR0824700.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHURQ_002976"
     CDS             complement(2346..3011)
                     /gene="yecA"
                     /locus_tag="ACHURQ_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR0824701.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3207..3293)
                     /locus_tag="ACHURQ_002977"
     tRNA            complement(3207..3293)
                     /locus_tag="ACHURQ_002977"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3257..3259),aa:Leu,seq:taa)
     gene            complement(3306..3379)
                     /locus_tag="ACHURQ_002978"
     tRNA            complement(3306..3379)
                     /locus_tag="ACHURQ_002978"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3345..3347),aa:Cys,seq:gca)
     gene            complement(3434..3509)
                     /locus_tag="ACHURQ_002979"
     tRNA            complement(3434..3509)
                     /locus_tag="ACHURQ_002979"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3474..3476),aa:Gly,seq:gcc)
     gene            complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHURQ_002980"
     CDS             complement(3661..4209)
                     /gene="pgsA"
                     /locus_tag="ACHURQ_002980"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR0824702.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHURQ_002981"
     CDS             complement(4266..6098)
                     /gene="uvrC"
                     /locus_tag="ACHURQ_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR0824703.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHURQ_002982"
     CDS             complement(6095..6751)
                     /gene="uvrY"
                     /locus_tag="ACHURQ_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR0824704.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7047..7223
                     /gene="yecU"
                     /locus_tag="ACHURQ_002983"
     CDS             7047..7223
                     /gene="yecU"
                     /locus_tag="ACHURQ_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR0824705.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7210..7434
                     /gene="yecF"
                     /locus_tag="ACHURQ_002984"
     CDS             7210..7434
                     /gene="yecF"
                     /locus_tag="ACHURQ_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR0824706.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="ACHURQ_002985"
     CDS             complement(7501..8223)
                     /gene="sdiA"
                     /locus_tag="ACHURQ_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR0824707.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8453..9205)
                     /gene="tcyN"
                     /locus_tag="ACHURQ_002986"
     CDS             complement(8453..9205)
                     /gene="tcyN"
                     /locus_tag="ACHURQ_002986"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR0824708.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="ACHURQ_002987"
     CDS             complement(9202..9870)
                     /gene="tcyL"
                     /locus_tag="ACHURQ_002987"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR0824709.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="ACHURQ_002988"
     CDS             complement(9885..10871)
                     /gene="dcyD"
                     /locus_tag="ACHURQ_002988"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR0824710.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         2713 a         2839 c         2578 g         2809 t
ORIGIN      
        1 attcgtattt ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa
       61 ttatagtgac gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag
      121 aaatgattga aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc
      181 agcaaatgag cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc
      241 gtcacgccca ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg
      301 gcaatttacc gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg
      361 aattattcca ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg
      421 ctcatgctgc aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt
      481 ctgagcagca tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag
      541 gcaaaagcgg cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa
      601 actaatgctc tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt
      661 actctgatcg gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa
      721 cgctaccagc tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc
      781 accaaaacgc gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc
      841 aatcatcata tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa
      901 gcctagcgta gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt
      961 tattgtacat ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc
     1021 ttaacctgcg ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa
     1081 cagaaccctg ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc
     1141 aatgccgctg gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct
     1201 ttgggcgttg atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc
     1261 agataccggt ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac
     1321 gggcttcagt atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg
     1381 tgaattgttg gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg
     1441 cgtgctgttg ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga
     1501 tttatttaac cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt
     1561 gctgctaccg catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc
     1621 tctgtcagca atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat
     1681 tgtcagctat atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc
     1741 gatccccctg gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac
     1801 aacctttatg ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg
     1861 cgaaatggtg gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct
     1921 cgccacgtca tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca
     1981 gcgttcaaat accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt
     2041 ggcgtttgca ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc
     2101 gctggcggta ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa
     2161 tcctcaggcg ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg
     2221 tggtattgct gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg
     2281 gtgatcagat agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg
     2341 gcctcttaat gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg
     2401 ttacgccccg gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc
     2461 gccatccagt acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca
     2521 aacgcttctg gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca
     2581 atcgcctcca gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg
     2641 ccccgcatat agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct
     2701 cgcaagccga ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg
     2761 gccatatgtt gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc
     2821 ggcacatagt cagccccacc ccacacggca accagccact gttccggttc aatctcttgc
     2881 ggagaactca acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg
     2941 ttagtgttgt atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt
     3001 cccgttttca tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg
     3061 gtccgtatca gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt
     3121 aacgacaagt tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg
     3181 ctttgatttt attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg
     3241 cgaacgccga gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa
     3301 gaaagtggag gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata
     3361 accgctttgt taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg
     3421 atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg
     3481 tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt
     3541 ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc
     3601 gatgagtcaa cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga
     3661 tcactgatca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt
     3721 cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg
     3781 ccacagcagc catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga
     3841 gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat
     3901 aatttcacgg gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc
     3961 ggttaccagc accatggcga tagccacgag aactttatct gctacagggt caaggaaagc
     4021 accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac
     4081 cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa
     4141 gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt
     4201 aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag
     4261 agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc
     4321 acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc
     4381 aacaacattt gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt
     4441 ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga
     4501 atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc
     4561 ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt
     4621 agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc
     4681 gcaagctggc ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg
     4741 gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg
     4801 atgcctgtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc
     4861 acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc
     4921 ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat
     4981 tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa
     5041 cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa
     5101 agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt
     5161 aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg
     5221 ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc
     5281 tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg
     5341 tcgaggtcgt cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga
     5401 atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata
     5461 cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac
     5521 tcaacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag
     5581 cgccctatct gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac
     5641 tggcgaatgg ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc
     5701 gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc
     5761 gggtgggtat cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg
     5821 ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct
     5881 gtatgcgtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc
     5941 gaagcgaggt tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg
     6001 acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg
     6061 gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat
     6121 tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt
     6181 tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg
     6241 agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag
     6301 acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt
     6361 gagcgatatc agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga
     6421 cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg
     6481 ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac
     6541 gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca
     6601 ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac
     6661 cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt
     6721 cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc
     6781 tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa
     6841 tggtgggtat tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac
     6901 gcccgtcacc aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa
     6961 aaattgactt tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac
     7021 ccgaacaata cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg
     7081 catttaatag cattgttgac gtcacgctca aaaaacaacc actgctattt ttagcaaagc
     7141 ttatggtata ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga
     7201 ggaaaataaa tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat
     7261 gaagtctcct gcctgaaagc gatgctgacg ctgatgctac aggcgatggg acaagctgac
     7321 gcgggccgcg tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag
     7381 gctgcagtat tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa
     7441 accggctgag agcgtgcctt tcagccgatt tttgcatctg gcacgcagaa agaaaagcga
     7501 tcaaattaag ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat
     7561 ttttttctgc atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat
     7621 cgctatctct gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt
     7681 gctgaaattc atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag
     7741 actttcgcgc accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc
     7801 gcgcgtgctg caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata
     7861 ctgagtgaca ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc
     7921 gctgaataag tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac
     7981 aggatcaatt gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt
     8041 ggtgtagaaa gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata
     8101 gtaatcgtac tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc
     8161 ctccatcctc tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg
     8221 catagtaaac cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc
     8281 acataaataa gatttatata taatttatat tattcaggca atgaattact tttgcaagcc
     8341 atcgcattct cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct
     8401 tgaaatggcg tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca
     8461 gcagaaactt ctcgaggaac tggcgggtgc gaggctgctg gggatcagca aataacgctt
     8521 ttgctgcccc ctgctcgact atccgcccct ggtccataaa aatcgcccgg tccgcaacat
     8581 cccgggcaaa gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct
     8641 ggcggattgt gttcaggact tcgcccacca gctctggatc cagcgctgac gttggctcgt
     8701 caaacaaaat cacctcagga cgcattgcca gcgcacgcgc aatcgctaca cgctgctgtt
     8761 gaccgccaga caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca
     8821 gcagctcgcg agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc
     8881 cttcaataat gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa
     8941 acccgacgtg ctgacgcaac tggcggatca gcgatttttg ctgacttaat gaacgtgcag
     9001 tatcaatagt gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta
     9061 tgctgcgtag caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc
     9121 caggctttac ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca
     9181 ggttcttaac ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc
     9241 aaaatggttc tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc
     9301 caggtacatg gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag
     9361 ctccggcacc tggattgtcg cggccagcga cgtatccttt accaggctga taaagctgtt
     9421 cgacagcggc ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg
     9481 ccatggcgtc ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat
     9541 ggcggcgcgc agtgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc
     9601 tgacggaatg ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag
     9661 ttgtgcgatg agcggtgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac
     9721 gggccagatt ggcgacaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa
     9781 catgccgccg atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga
     9841 atcaataacc agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga
     9901 taggcgaaca gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg
     9961 aagcgtttct gactgataca gccaatcagc cccgccatcg cttttccggt atacacagga
    10021 tcaagcagaa tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc
    10081 ggcacgccgt agccaggtgc aaaatagtca tcccagagta taatttccgc tgatgcggtc
    10141 agctccagtt ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ctgatcggca
    10201 acggaacgcg acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca
    10261 acagccagtc cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta
    10321 accgcccctt cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca
    10381 agagcattag aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc
    10441 gtcgccagct cttccagttg ggcattggga tcggtcagtg cttcgcacat ttcaatctgg
    10501 gtattgaaca gatccagcaa caagcgattg ccgttggtta aatagttttc tgcggttgtg
    10561 ccaataggat tttccagcag cgccacacag tgcagaccga gtttcgccgc aactgcggca
    10621 gtctgccgca catggttaga ctggatcacc ccggcagtaa tcagcgtatc ggcaccttcg
    10681 cgcagagcat ctgccgcgag aaattctagc ttacgtaatt tattgccgcc cattgccatg
    10741 ggggtgacgt catcccgttt gatgaaaatt tcccgtccta gataatcaga aaagcgcggc
    10801 agatattcga gcggcgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta
    10861 tgcagtggca taacagcctc cgatgtatgt tgttgtgatt ttcttattat gcacgctgaa
    10921 aacgcgtaaa taaaaaagg
//
LOCUS       DAZOZY010000135        10853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZOZY010000135 DAZOZY010000000
VERSION     DAZOZY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.135"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            80..421
                     /locus_tag="ACHURQ_002989"
     CDS             80..421
                     /locus_tag="ACHURQ_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR0824711.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(412..1338)
                     /locus_tag="ACHURQ_002990"
     CDS             complement(412..1338)
                     /locus_tag="ACHURQ_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0824712.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            1428..2426
                     /locus_tag="ACHURQ_002991"
     CDS             1428..2426
                     /locus_tag="ACHURQ_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR0824713.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2423..2641)
                     /locus_tag="ACHURQ_002992"
     CDS             complement(2423..2641)
                     /locus_tag="ACHURQ_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR0824714.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2643..4658)
                     /gene="ligA"
                     /locus_tag="ACHURQ_002993"
     CDS             complement(2643..4658)
                     /gene="ligA"
                     /locus_tag="ACHURQ_002993"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR0824715.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4729..5715)
                     /gene="zipA"
                     /locus_tag="ACHURQ_002994"
     CDS             complement(4729..5715)
                     /gene="zipA"
                     /locus_tag="ACHURQ_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR0824716.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            5945..6706
                     /gene="cysZ"
                     /locus_tag="ACHURQ_002995"
     CDS             5945..6706
                     /gene="cysZ"
                     /locus_tag="ACHURQ_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR0824717.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            6891..7862
                     /gene="cysK"
                     /locus_tag="ACHURQ_002996"
     CDS             6891..7862
                     /gene="cysK"
                     /locus_tag="ACHURQ_002996"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR0824718.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8246..8503
                     /gene="ptsH"
                     /locus_tag="ACHURQ_002997"
     CDS             8246..8503
                     /gene="ptsH"
                     /locus_tag="ACHURQ_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR0824719.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8548..10275
                     /gene="ptsI"
                     /locus_tag="ACHURQ_002998"
     CDS             8548..10275
                     /gene="ptsI"
                     /locus_tag="ACHURQ_002998"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR0824720.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10316..10825
                     /gene="crr"
                     /locus_tag="ACHURQ_002999"
     CDS             10316..10825
                     /gene="crr"
                     /locus_tag="ACHURQ_002999"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR0824721.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2641 a         2797 c         2691 g         2724 t
ORIGIN      
        1 gatttgtaag attgattttt cttctcaagt tccttgttct tctgccgata ttacctcgta
       61 cagtgacgag gactgaacaa tggccactat ctcttcaact tcaattcctt ccatacaaac
      121 acagagtagt aacagggcat cacaaggtag cgatgttgct tcaaaaatcg cgcggataag
      181 tcagcaaatc atcaaactca ctcaacaaat taaagagatt gtagacacta gcggcagtgc
      241 tgaagacaaa caaaaacagg ccgaattgat tcaacagcaa atcaccctac tggaaacaca
      301 actggcgcag ttgcagagac agcaggcaga aaaggcgcag gagaaagagc agcgtttgtc
      361 tctgaatgta tcccttctaa atcctgttga aaatacgact catatcgata tctatatctg
      421 atacaacgga gccccttccc aaccagccat tagcgcattg ccctggtctc gtaccacgtc
      481 ccataacgct tcagcggcgg tggaaagtga acggttcttc ctgcgcacca gcatcaactg
      541 gcgttcaaca accggcgtaa tgcgctttac caccagtggg ctaccttcag gcaatggtag
      601 cgccagtgcg ggaagaatac tgatgccgat gcctgcggct accatcggga acagcgtcgc
      661 cggatgtcca atctcctgca caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc
      721 aatcagcggt cggctgcctg acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc
      781 ctgccagggt acataatctt ccacggccaa agcgctatcg cgatggcaaa gcagaaagaa
      841 aggctcggaa agaatcgctt cacattgcag atcgcccact gggcctggat cgatgacgat
      901 gcaaaaatcg acatctccct ggcgaatact ttccatcacc cactgctgcg gacgatcgtg
      961 caggacaaac tgaatatctg gatagcggcg atggctttca gcaatgcatt gcggaataag
     1021 atgcgcggaa atggtctggc tggcagcgac ccgaactttt ccgctcagtt gttgccccat
     1081 acgcccggta tcgcgcaacg tgctgttaag ttcatccagc agtcgctcaa gacgcaaagc
     1141 caactgctgc cctgcatctg taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac
     1201 accagtatga ttttccagct ccttcacact gtgacttact gccgactggc tcaagccgat
     1261 acgctctcct gcacgactaa aacttttctc ctgcgctact gtgacgaaaa cctttaattg
     1321 ttttaaagaa taattcatct gttttcttca tgaatggatg caataaatca attttatttc
     1381 tcaaactgga agaagcacaa tagaaccatc gatcatctgg agtctttatg aaactttttc
     1441 gtatcctcga tcctttcacc ttaaccctga tcacggtggt gttgctggcc tctttctttc
     1501 cggccagagg cgatttcgtc cccttctttg aaaatctgac caccgcagct attgccctgc
     1561 tgttctttat gcacggcgcg aagttgtcgc gtgaggcgat tattgctggc ggtggtcact
     1621 ggcgactgca tttgtgggta atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac
     1681 tgtttgcctg gtggaaaccg gtaaatgtcg acccgatgct ctactccggt tttctctact
     1741 tgtgcattct cccggctacc gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta
     1801 acgtcgcggc ggcggtttgt tctgcgtcag catccagcct gctggggatt ttcctttcac
     1861 cattgctagt tggtctggtg atgaatgttc acggtgcagg gggcagcctt gagcaggtcg
     1921 gtaaaattat gctgcaactg ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga
     1981 ttggtgactg ggtgtcgcgc aataaaaaat ggattgcgaa aactgaccag acgtccattc
     2041 tgttggtggt ttatacagcg ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg
     2101 gctggggatc attgctgttt atcgtggtgg tcagctgcgt tcttctggct atcgtgattg
     2161 tagttaacgt ctttatggca cgccgactgg gcttcaataa ggcagatgaa attactatcg
     2221 tcttttgtgg ttcgaaaaag agtctggcaa atggcatccc gatggcaaac attctgttcc
     2281 ccacatcggt gatcggtatg atggtgctgc ccctgatgat tttccatcag atccaattga
     2341 tggtctgtgc ggtgctggcg cgtcgataca aacgccagac cgaacagtta caggcgcagc
     2401 aggaaagcag cgccgataaa gcttaaagcg gacgcttcag gggctggacc agttgcgtca
     2461 gcccctcggt tttaatcagc agcgtgattt gcattagctc accgagcttc cctgccggga
     2521 actcatcttt gcgggcaaac cacagcagat actcctccgg caggtcgatt aagcgacgcc
     2581 ctttgtattt gccaaacggc attatcgtat tggctatttc aatcagctgc tctttttcca
     2641 tctcagctac ccagcaaacg cagcatttcc gcttcgtcga tgacttcaat gcccagttcc
     2701 tgcgccttcg ccagtttaga tcctgcagct tcacccgcta tcaccagatc ggttttcttc
     2761 gacacgctgc ccgcgacttt cgcgcccagt tcgaccagtc gagctttagc gtcatcacgc
     2821 gacatctggc ttaagctgcc cgtaagcacc acggttttac cagcaaacgg gctgtcaatc
     2881 tcttccgcgt tgataacgat cggcgcaggc cagtgaacac cttccgccaa cagctcgctg
     2941 atgacattgc ggttgctttc ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg
     3001 ccaacatcag gcaccttttg cagctcttca atcgaagcgg cttccagcac ttccagcgtg
     3061 ccgaaatatg ccgccagacc tgctgcggtg gcctcgccga cttcacggat gccaagtgca
     3121 tagaggaagc gagcaaaggt ggtttctttc gctttttcca gcgcgttaac cacgttttgt
     3181 gccgattttg gccccatacg ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga
     3241 tctgccggag tgtggacata ttctttttca accagctgat cgatgatttt gtcgcccatt
     3301 ccgtcaacat ccatcgcacg gcgggaaaca aagtgtttca gcgactcttt acgctgcgca
     3361 ccgcaaatca ggccaccggt acagcgggca accgcttcac cttccacacg ctcaacgtca
     3421 gaaccacata ccggacaatg cgtcgggaat acaacctcac gggtatcttc cgggcgttca
     3481 gaaagcacga cgttaaccac ctgcgggatc acgtcgccag cgcggcgaat caccacttta
     3541 tcgccaatgc gtaaaccaag acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc
     3601 accagcacgc cagcaacatg gacaggttcc agacgcgcaa caggcgtaat cgcgccagta
     3661 cgcccaacct gaaactcgac gtcacgcaca aaggtcatct gctcctgcgc cgggaattta
     3721 aacgctaccg cccagcgcgg ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt
     3781 gagttgacct taatcaccac gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc
     3841 actttgtgat agaacgccag cacttcttcc gccgattcac aaagcgttac ccgatcgctg
     3901 accggcaacc cccacttttt aaattgcagt aaacggccaa gatgagtatc cggcagctcg
     3961 ccaccttcca gaacaccaac gccatagcag aaaaaagtga gcggtcgctt cgctgtaata
     4021 cgcggatcaa gctgacgcag tgaaccagct gccgcattac gtgggttagc aaaaactttc
     4081 ccgcccgtgc gtcgcgcatc ttcgttaatc ttttcgaacc ccgcctgcgg caggaacact
     4141 tcaccacgca cttccagacg cgccgggata ttctctccgt gcagcttcag cggaatggcg
     4201 cgaatagtac gcacattaga cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg
     4261 gcactgacta aaacgccatt ttcatacaga atactgacgg caaggccatc cagcttcagc
     4321 tcacagcacc aggtgacttt ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa
     4381 gcaagaaagc tttcttcatc aaaaacgtta tccagtgaca gcattggtac ttcatggcgt
     4441 atctggctga aagccgccag cggcgcagca cctacacgtt gggtaggcga atcaggcgta
     4501 atcagttctg gatgtttggt ttccagctcg cgcagttcgc gcatcagcct gtcgtattca
     4561 gcgtcgggaa tttccggcgc atccatcaca tgataaagat attcatgatg gcgaagcgtc
     4621 gttcgcagtt ctgtcagttg ttgttcgatt gattccatat cgcaccatca atgctaaaaa
     4681 cccccgacaa gcgggggttc gaagaggagt taatttgcct taagtgtatc aggcgttggc
     4741 gtctttgact tcgcggatga tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg
     4801 gcgctgatcg tcaagcacga caccgcccac ttcatcggca atatgctgcg cagattgcag
     4861 catcagcttg aagttctgca gctcgtcacc gtaagacggt acctgcataa agatagtgac
     4921 acccggagta gtgaaatcct tcatttcagg atcaaaggtt cccggtttca ccatattcgc
     4981 caggctgaat aacgccgggc cgctgccatc cgggctaaga tgacgatggt aaatattcat
     5041 atcgccaaaa atgaagcccg cttgttgaat gctgttaaga agcagttcac cgtttagctc
     5101 gctaccgtga tgcgccgcga cgttcatgat aatcaccgct tctttgcgct tcggtttatc
     5161 cataactgga gccggttccg ctacaggctc aggctgtggt gccgctacgg gttctgcagg
     5221 ctggaaagcc tgttgtgccg gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac
     5281 ctgtggcgaa actggctgct gcaacggctg ttcaggctgc ggctgatagg caggctgctg
     5341 catcggctgc gctggacgcg gagcatgttg cggcggtacc tgcgcttcag gcggctgctg
     5401 gatcggttga cgcggctgcg cagacgcata aggcggttgg tactggtgtt gcggcgacgg
     5461 acgagcagcc tcatgctcct gagcgttagc cggggcatga ttcacgcggt gaacacgaac
     5521 ctcaccaacg ccctcatcat cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga
     5581 cttcattcgt tttaatggcc gatcgcggaa catagaagat cgttctttac ggctggtcca
     5641 gaaaccatgt accagtaaag cgattatggc gatcgcgcca acaatgatta atatcagacg
     5701 caaatcctgc atcattatat tctctgttgt tctaacacct tgccaccacg gcaaacattt
     5761 actcactaag agtatttgcc gattacctca agtgcaagtg cactattaac tttcacagca
     5821 caaagataga tgaaatcgtg ctttttgctg ttttttcgaa catatcctaa ctgtccattg
     5881 cgcaattacc cggtaaaata cgcagaattt tcctgggatt ggtcaaaagg agctcatcct
     5941 gattatggtt tcatcattca catctgcccc acgcagcggt ttttactatt ttgcgcaagg
     6001 ctggaagctc gtctcgcaac ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa
     6061 tattttgttg atggggggcg cattctggtg gctctttaca cagctcgatg tctggatccc
     6121 gactctcatg agttacgttc cggactggct acaatggctg agttatctgt tgtggcctct
     6181 ggctgtcatc tctgtgctgt tagtgtttgg ctatttcttc tccacgattg ctaactggat
     6241 tgccgctccg tttaacggtt tattggctga acaactggaa gcacgattga ctggcgctac
     6301 accgccagat accgggattt tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga
     6361 atggcaaaaa tttgcctggt atctgccgcg cgcaattgta ttactaattc tttacttcat
     6421 ccctggtatt gggcaaaccg tcgcgccggt actgtggttc ctgtttagcg cctggatgtt
     6481 agccatccag tattgcgatt accccttcga taaccacaaa gtgccgttta aagagatgcg
     6541 caccgccctg cgcacacgca aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt
     6601 tacgatgatc ccgctgctta atctgttcat catgcccgtt gccgtttgtg gcgcgacggc
     6661 gatgtgggtc gattgctatc gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta
     6721 ttttgtaaac cgtttgtgtg aaacaggggt ggcttatgcc gccccttatt ccatcttgca
     6781 tgtcattatt tcccttctgt atatagatat gctaaatcct tacttccgca tattctctga
     6841 gcgggtatgc tacctgttgt atcccaattt catacagtta aggacaggcc atgagtaaga
     6901 tttttgaaga taactcgctg actatcggtc acacgccgct ggttcgcctg aatcgcatcg
     6961 gtaacggacg cattctggcg aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc
     7021 gtatcggtgc caacatgatt tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg
     7081 aactggttga accgaccagc ggtaataccg ggattgcact ggcctatgtt gctgccgctc
     7141 gcggttacaa actcaccctg accatgccag aaaccatgag tattgaacgc cgcaagctgc
     7201 tgaaagcgtt aggtgcaaac ctagtgctga cggaaggtgc taaaggcatg aaaggcgcaa
     7261 tccaaaaagc agaagaaatt gtcgccagca atccagagaa atacctgctg ctgcaacaat
     7321 tcagcaatcc ggcaaaccct gaaattcacg aaaaaaccac cggtccggag atctgggaag
     7381 ataccgacgg tcaggttgat gtatttattg ctggcgttgg gactggcggt acgctgactg
     7441 gcgtcagccg ctacattaaa ggcaccaaag gcaagaccga tcttatctct gtcgccgttg
     7501 agccaaccga ttctccagtt atcgcccagg cgctggcagg tgaagagatt aaacctggcc
     7561 cgcataaaat tcagggtatt ggcgctggtt ttatcccggc taacctcgat ctcaagctgg
     7621 tcgataaagt cattggcatc accaatgaag aagccatttc taccgcccgt cgtctaatgg
     7681 aagaagaagg tattcttgca ggtatctctt ctggagcagc tgttgccgcg gcgttgaaac
     7741 tacaagaaga tgaaagcttt accaataaga atattgtggt tattctacca tcatcgggtg
     7801 agcgttattt aagcaccgca ttgtttgccg atctcttcac tgagaaagaa ttgcaacagt
     7861 aatgccagct tgttaaaaat gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg
     7921 cttcaaactt tcgcccctcc tggcattgat tcagcctgtc ggaactggta tttaaccaga
     7981 ctaattattt tgatgcgcga aattaatcgt tacaggaaaa gccaaagctg aatcgatttt
     8041 atgatttggt tcaattcttc ctttaacggc ataatgttta atgacgtacg aaacgtcagc
     8101 ggtcaacacc cgccagcaat ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa
     8161 ctggcgctaa caatacaggc taaagtcgaa ccgccaggct agactttagt tccacaacac
     8221 taaacctata agttggggaa atacaatgtt ccagcaagaa gttaccatta ccgctccgaa
     8281 cggtctgcac acccgccctg ctgcccagtt tgtaaaagaa gctaagggct tcacttctga
     8341 aattactgtg acttccaacg gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac
     8401 tctgggcctg actcaaggta ccgttgtgac tatctccgca gaaggcgaag acgagcagaa
     8461 agcggttgaa catctggtta aactgatggc ggaactcgag taatttcccg ggttctttta
     8521 aaaatcagtc acaagtaagg tagggttatg atttcaggca ttttagcatc cccgggtatc
     8581 gctttcggta aagctctgct tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt
     8641 tctgccgacc aggttgatca ggaagttgaa cgttttctga gcggtcgtgc caaggcatca
     8701 gcccagctgg aaacgatcaa aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc
     8761 atctttgaag ggcatattat gctgctcgaa gatgaggagc tggagcagga aatcatagcc
     8821 ctgattaaag ataaacacat gacagctgac gcagctgctc atgaagttat cgaaggtcag
     8881 gcttctgccc tggaagagct ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt
     8941 gatatcggta agcgcctgct gcgcaacatc ctgggcctga agattatcga cctgagcgcc
     9001 attcaggatg aagtcattct ggttgccgct gacctgacgc cgtccgaaac cgcacagctg
     9061 aacctgaaga aggtgctggg tttcatcacc gacgcgggtg gccgtacttc ccacacctct
     9121 atcatggcgc gttctctgga actgcctgct atcgtgggta ccggtagcgt cacctctcag
     9181 gtgaaaaatg acgactatct gattctggat gccgtaaata atcaggttta cgtcaatcca
     9241 accaacgaag ttattgataa aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca
     9301 gagcttgcta aactgaaaga tctgccagct attacgctgg acggtcacca ggtagaagta
     9361 tgcgctaaca ttggtacggt tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc
     9421 gttggtctgt atcgtactga gttcctgttc atggaccgcg acgcgctgcc cactgaagaa
     9481 gaacagtttg ctgcttacaa agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt
     9541 cgtaccatgg acatcggcgg cgacaaagag ctgccataca tgaacttccc gaaagaagag
     9601 aacccgttcc tcggctggcg cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc
     9661 gatcagctcc gcgctatcct gcgtgcctcg gctttcggta aattgcgcat tatgttcccg
     9721 atgatcatct ctgttgaaga agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag
     9781 gaactgcgcg acgaaggtaa agcgtttgac gagtcaattg aaatcggcgt aatggtggaa
     9841 acaccggctg ccgcaacaat tgcacgtcat ttagccaaag aagttgattt ctttagtatc
     9901 ggcaccaatg atttaacgca gtacactctg gcagttgacc gtggtaatga tatgatttca
     9961 cacctttacc agccaatgtc accgtccgtg ctgaacttga tcaagcaagt tattgatgct
    10021 tctcatgctg aaggcaaatg gactggcatg tgtggtgagc ttgctggcga tgaacgtgct
    10081 acacttctgt tgctggggat gggtctggac gaattctcta tgagcgccat ttctatcccg
    10141 cgcattaaga agattatccg taacacgaac ttcgaagatg cgaaggtgtt agcagagcag
    10201 gctcttgctc aaccgacaac ggacgagtta atgacgctgg ttaacaagtt cattgaagaa
    10261 aaaacaatct gctaatccac gagatgcggc ccaatttact gcttaggaga agatcatggg
    10321 tttgttcgat aaactgaaat ctctggtttc cgacgacaag aaggataccg gaactattga
    10381 gatcattgct ccgctctctg gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt
    10441 tgcggaaaaa atcgttggtg atggtattgc tatcaaacca acgggtaaca aaatggtcgc
    10501 gcctgtagac ggtaccattg gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc
    10561 tgatagcggc gttgaactgt tcgtccactt cggtatcgac accgttgaac tgaaaggcga
    10621 aggcttcaag cgtattgctg aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga
    10681 atttgatctg ccgctgctgg aagagaaagc caagtctacc ctgactccgg ttgttatctc
    10741 caacatggac gaaatcaaag aactgatcaa actgtccggt agcgtaaccg tgggtgaaac
    10801 cccggttatc cgcatcaaga agtaattctt gccgcagtga aaaatggcgc cca
//
LOCUS       DAZOZY010000136        10835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZOZY010000136 DAZOZY010000000
VERSION     DAZOZY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.136"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..279)
                     /locus_tag="ACHURQ_003000"
     CDS             complement(<1..279)
                     /locus_tag="ACHURQ_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0824722.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(446..1186)
                     /locus_tag="ACHURQ_003001"
     CDS             complement(446..1186)
                     /locus_tag="ACHURQ_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR0824723.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHURQ_003002"
     CDS             complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHURQ_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR0824724.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHURQ_003003"
     CDS             complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHURQ_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR0824725.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHURQ_003004"
     CDS             complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHURQ_003004"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR0824726.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHURQ_003005"
     CDS             complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHURQ_003005"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR0824727.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHURQ_003006"
     CDS             complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHURQ_003006"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR0824728.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7002..7433
                     /locus_tag="ACHURQ_003007"
     CDS             7002..7433
                     /locus_tag="ACHURQ_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR0824729.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7456..8034
                     /locus_tag="ACHURQ_003008"
     CDS             7456..8034
                     /locus_tag="ACHURQ_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR0824730.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8035..8742
                     /locus_tag="ACHURQ_003009"
     CDS             8035..8742
                     /locus_tag="ACHURQ_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR0824731.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8730..9407
                     /locus_tag="ACHURQ_003010"
     CDS             8730..9407
                     /locus_tag="ACHURQ_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0824732.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9401..10078
                     /locus_tag="ACHURQ_003011"
     CDS             9401..10078
                     /locus_tag="ACHURQ_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR0824733.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10050..10763)
                     /locus_tag="ACHURQ_003012"
     CDS             complement(10050..10763)
                     /locus_tag="ACHURQ_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR0824734.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10760..>10835)
                     /locus_tag="ACHURQ_003013"
     CDS             complement(10760..>10835)
                     /locus_tag="ACHURQ_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0824735.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2580 a         2880 c         2699 g         2676 t
ORIGIN      
        1 tcgtgaacga cgccctgagt tatggatttc attatcaatc gcagaaagtc gttgataaac
       61 cgggaacagt ttttgataca gttcctgacc ggctttattg aggctgatat tattatcttt
      121 gcgggtaaat agcgtatagc caatttgttc ttccagcgcg gcaatgcttt taccaaatgg
      181 cgaagccgtc atatggattt tttctgccgc ccgggcaata ttattggttt gcgctaataa
      241 aatgaaattg cgcatctttt tcgaaataaa aatgtccatc ccccctcccc gcatccttgt
      301 ccgcttaacc gtttcagtca gcctatcctt gatgaaaccg cgagcaaaga taggtgatta
      361 cgtcatggtt ttacagaaaa ttacagaaaa aggaggcaat atcgggtaaa ggcattagcc
      421 cgacgaatac gtcgggctac aagtattatt gtgctgcagg tgttttagcg ggttgttgat
      481 ccacaggttc taactggaag accacatcga cctgatcatc aaactgaata gcggcctgct
      541 cgtaagtttc ctgggcagac accggcgcgg catcggcttt catcatccgc accattgggc
      601 tgggctgata gttggaaaca tggtagcgca cgctatatac cggccccagt ttacgatgaa
      661 agccgttcgc cagttcctgc gcctgatgaa tcgcgttatc aatcgctgcc ttacgcgctt
      721 tgtctttata ggcatccggc tgcgccacgc ccagcgacac agaacgaatt tcgttaagac
      781 ccgccttcag cgcgccatcc agcaaggaat tcagtttgtc taactgacgg agcgtgactt
      841 ccaccgttct cacagcgcgg tagcctttca ggatactttt accatcctga taatcataat
      901 ctggctgggt gcgtaagttc gctgagctga tatctttttt cgcgatctga ttgagttcaa
      961 ggaaggaaat gtattgtgcg acgcgctcat ctgcctgttt cttggctgta gcggcatcct
     1021 tcgcggccac gttaacttca atcgcaagag tggcaatgtc tggcaccgca tccacacttg
     1081 ccgtaccgga ggtgacaata tgcggtccat ccggcaattc gttagcctgc gctgccatcc
     1141 cgctaatacc cattaatgcc gccagggcga taactttgaa cttcacgtta gtctctccgt
     1201 gttaattgac ttgcccaaaa ttcaggacct ctggaggcaa gcttagcgcc tctgttttat
     1261 ttttccatca gatagcgcct aactgaacaa ggcttgtgca tgagcaatac cgtctctcgc
     1321 cagctgcaag gctataaacc acatgacaca tcccactacc agattgataa tgcgctgtga
     1381 ttttgccgtg cgcaggcgcg gtgccagcca ggctgcgaga atagccagac caaagaacca
     1441 caggaaagag gcgctaattg tcccgagcgc aaaccaacgt tttggttcga cgtcaagctg
     1501 cccgccaagg ctgcccagta caacaaaagt atccaggtaa acatgcggat tcagccaggt
     1561 cactgccagc atggtggcga taattttcca tctgccttgc ttcaggactt cggcactagc
     1621 taactcaata ttactactca tcgctgtttt aaaagcgcca aaaccatacc acagcaagaa
     1681 ggctacgccg ccccaggtga ccagcgccag caaccacggc gactgcatca ataacgcgct
     1741 gccaccaaaa atcccggcgc aaatcaggac caaatcgctg atagcacaaa gtaaggcaat
     1801 cataatgtgg tactgacgac gaatgccctg attcatcaca aaagcatttt gtggaccgag
     1861 cggcagaatc atagctgccc caagagcaat accttgaaag taataagaaa acacgttgga
     1921 tattccgaat taagtatctg atgcagagac tatatcgcgg gattgttatt agcggaaatt
     1981 gataatatta attggcaatc agaataacta ataagagaga ggccagagag tattatctgg
     2041 cctcagcgtt gattacgcag ctttgtcttc tttcacccgc ttaaagttca catccatttg
     2101 cgggtacggg aagctgatac cggcggcatc aaattcacgt ttaatacgct ccagcacatc
     2161 ccagtacacg ttttgcagat cgccgctgtt gctccagacg cggaccacga aattaatcga
     2221 cgatgcacca agttcgttca gacgcacagt catttcgcga tctttcaaaa tgcgatcttc
     2281 agactggata atattggtca ggatctgctt aacctgatcg atatcggaat catacgccac
     2341 gccaataata aattcgttac ggcgaactgg ctcgcgagag aagttaataa tatttccggc
     2401 aataatttta ccgttcggaa taacgataat tttaccgtct gcagtacgca tggtggtgga
     2461 gaaaatctgc acactcagca cagtaccggc tacgccgccg agatcaacat attctccggc
     2521 acggaacggg cggaacatga caagtaacac gccagcggcc aggttagaaa gtgacccctg
     2581 caaagccaga ccaacagcta agcctgcggc accgagtaca gcaatgactg acgctgtttg
     2641 tacacccacg cgtcccaggg cagcgattag cgtaaaggcg ataataccgt aacggactaa
     2701 tgcagaaaga aaatcagcaa cagtggcatc gattttacgg gagatcatca ggcgattcac
     2761 cgcgttggaa atcatccgcg cgataatcaa accaacgatg atgatcgcga gtgccgccac
     2821 gatgtttact gcataactta gcagcagcgc ctggttagct accagccagc ttcccgcgcc
     2881 gtttatgcta tcgacaacat tcaaatcttc cattcaatat tccttttcaa ctgactcaaa
     2941 atggagaaat acactccgcc gccttatgac gggcagtctg acaagggtaa acaataaagc
     3001 ataattttgc caaatagatc acagatttct catctgaatc accttgcttt tggcgaaaaa
     3061 aagacccgca gagcgggcct tgagataagc agaaaggaat atcttacaga acgtcgatcg
     3121 cgttcagttc ctggaatgct ttttccagac gagcgatcat cgaagtctga ccggcacgca
     3181 gccatacgcg cggatcgtag tatttcttgt tcggctgatc ttcgcctttc gggttaccca
     3241 actgcccctg cagataagct tcgttcgctt tgtagtagtt cagaacgcct tcccaggttg
     3301 cccattgggt atcggtatcg atgttcattt ttactacgcc gtagcttacg gagtctttga
     3361 tttcctgagc agtagaaccg gaaccaccgt ggaatacgaa gttcaggctg ttgtgcggca
     3421 ggttgtgttt cttggaaaca tattcctgag aatcacgcag gatggtcgga gtcagaacca
     3481 cgttacccgg cttgtaaaca ccgtgtacgt taccgaagga cgcagcgatg gtgaaacgcg
     3541 ggctgatttt gctcagttcg gtgtatgcgt aatcaacgtc ttccggctgg gtgtacagtg
     3601 cagaagcgtc catgtggctg ttgtccacgc cgtcttcttc accaccggtg caacccagtt
     3661 cgatttccag agtcatgccg attttggaca tgcgctccag gtatttagag cagatttcga
     3721 tgttctcttg cagagattct tcagacaggt cgatcatgtg agaagagaac agcggcttac
     3781 cggtagctgc gaagtgtttt tcacccgcgt ccagcagacc gtcgatccac ggcagcagtt
     3841 tcttcgcgca gtggtcagtg tgcaggataa ccggaacacc ataatgttca gccatctggt
     3901 gaacgtgatg cgcaccagag atcgcgccca ggatagcagc accctgcgga acgtcagatt
     3961 tcacgccttt accagcgata aaggaagcac caccgttgga gaactgaacg ataaccggcg
     4021 ctttaacttt agcagcggtt tccagtacgg cgttgatgga gtcagtaccg acgcagttta
     4081 ctgctggcag tgcgaagttg ttttcttttg ctacctggaa aactttctgt acgtcatcac
     4141 cagtgattac gccaggtttt acgaaatcaa aaatcttaga catgtctctt gtcctgtatc
     4201 gtcgggcctt atactcatca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc
     4261 cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg
     4321 aattatttag agtatgaaaa aatgcgtgct ctaaaagcgc gctgaaacaa gggcaggttt
     4381 ccctgccctg tgatttttta cttcttagcg cgctcttcga gcatcgctac tgcaggcagt
     4441 actttacctt ccacgaattc gaggaatgcg ccgccgccag tggagatgta ggagattttg
     4501 tcagcaatgc cgaacaggtc gattgctgcc agagtgtcgc cgccgccagc gatggagaac
     4561 gcttcgctgt ctgcgatagc gttagccacg atttcagtac ctttgcggaa gttcgggaat
     4621 tcgaacacgc caaccggacc gttccacaga atggttttcg cattcttcag gatttcagcc
     4681 agttcctgag cggaagcatc accgatatcc aggatctgct cgtcagcttt cacatcgtta
     4741 acagatttca gggtagccga tgcagtttca gagaactcgg ttgctacgcg aacatcagac
     4801 ggaaccggga tgttgcaggt ggtcagcaga cgtttagctt cgtcaaccag gtcagcttcg
     4861 tacagggatt tacccacatc gtggccttgt gccgcgataa aggtgttagc gataccacca
     4921 ccaacaatca gctggtcagc gattttagac agggagtcca gaacggtcag tttggtagat
     4981 actttagaac caccaacgat agccaccatc gggcgagcag gttctttcag tgctttaccc
     5041 agcgcgtcca gttcagctgc caacagcggg cctgcgcacg caacatcagc gaatttaccg
     5101 ataccgtgag tagaagcctg cgcgcggtga gcagtaccga atgcgtccat tacgaacacg
     5161 tcacacagtg cagcgtattt tttggacagg gtttcgtcgt ctttcttctc gcctttgttg
     5221 aagcgaacgt tttccagaac aaccagttca ccttcagcaa cgtcaacgcc gtcgaggtaa
     5281 tctttaacca gacgaaccgg gttagacagt ttgtctttca ggtagttaac aaccggcagc
     5341 agagagaatt cttcgttgta ctcgccttcg gtaggacgac ccaggtggga agttaccatc
     5401 actttcgcgc cttgtttcag ggccagttcg atggtcggca gagaagcacg gatacgcgcg
     5461 tcgctggtta ctttcccgtc ttttactggt acgttcagat ccgcacggat aaatacacgt
     5521 ttcccagcaa gatccagatc ggtcatctta attacagaca tggtgaatcc tctcgttgat
     5581 tctaaaagtt ttgcagacgc tgcttgcgtc ttacctgaaa gcaacagtag ccatagctaa
     5641 cgtcgtgtcg agcattcggt tagcaaagcc ccattcgtta tcgcaccaga ccaacgtttt
     5701 gatcaggtgt gcgccactga cccgggtttg ggtgccatcg acaatggcac tgtgcggatc
     5761 gtggttaaaa tctacagaga ccaacggcaa ttccgtatag tcaactatac catgaaatgc
     5821 accttgtgct gctttttgca gcaacaggtt gacttcattg gcttttacag gtttcttcac
     5881 cgtcacgctt aaatcgattg ccgtcacatt tatggttggc acacgtaccg caatcgcttc
     5941 aaagcgatcg ttaaattgcg gaaaaaatcg tgtgataccg gcggccagtt tagtatcgac
     6001 cggaatgatc gactggctgg ctgcccgggt gcgacgcagg tcaggatggt atgcatcaat
     6061 aacctgttga tcgtgcatgg cggagtgaat tgtggtcaca gtgccggact caataccgta
     6121 cgcatcatct aacaatttga tgacgggaat tatgcaattc gtggtacagg aagcgttaga
     6181 aacgatgcgg tgttccgcac gaagttgatc ctgattgacg ccgtaaacaa cggtcgcgtc
     6241 gagatcgtta ctgccaggat gtgaaaagag cacttttttg gccccggcgg caatatgcgc
     6301 ttcgccatgc tcgcgggagc catatacgcc ggtgcagtcg aggactacat caacgccaag
     6361 ttcacgccag gggagcgatt gcagtgaacg ttcatgcaat acgcggatgg cgtcatcacc
     6421 aacaaaaagt tgatcgcgtt cctgtcgtac ttcccatgca aaacggccat ggctggtgtc
     6481 atatttcaac aaatgcgcca tgcccgcagc atccgccagt tcgttgattg ccaccacggt
     6541 aatttccgcc cggcgtccgg attcatacaa agcacgaacc acattacgcc cgatgcgacc
     6601 gaagccattt atcgctacgc gtacggtcat agatctcctg caaggttttc cctgagcaaa
     6661 tttgccagac agagtaatcc agcaaatcgt ccggggaaac cttacctgtc gcaaactgcg
     6721 actgattggt taattgtcga acatttaatc gactgaaacg cttcagctag gataagcgaa
     6781 acgtggaata aaaggaatga ttgtccagtc aaagaaaaca attatctgac ttacgtcacg
     6841 ttttcagccc cgcccctgtg gaatttattc cactttgcag aatcacgaat aagcgtagca
     6901 aaatcttttc gttgtgccgt cacaaaccct gacttatatc agaatatgac tgatgcagcg
     6961 tcgatattat ccgcaatacg tccgagtttc ggagtactcc aatgatgaca ttgccctacc
     7021 catattcttc ccccgcacgc ttttatgcac tgcgcctcct ccccgggcag gaagtttttt
     7081 cccagctaca cgcttttgca cagcagcatc aacttcatgc cgcctggatt gcaggctgta
     7141 ccggtagcct gactgacgtc gccttgcgct atgccgggca agaaggcacc acgctcctta
     7201 acggtacatt cgaagtgatc tcactaaacg gcacgctgga acagagtggt gagcatcttc
     7261 atctctgcgt ttccgatcca cacggcacga tgcttggcgg tcatatgatg cccggctgta
     7321 ccgtgcggac cacgcttgaa ctggtgatcg gtagccttga ggaactggcg tttagccgcc
     7381 aaccttgcgc gctttctggt tacgacgagt tgcatatttc ccccgttaaa taactacttt
     7441 caaagaggtt ttcttatggc gcgtagccac ttttccagtc aggcactggt gttgatcgtt
     7501 atatctatcg ccatcaatat gatcggcggg caactggcca gcatggtcaa attacctata
     7561 tttctcgact ccatcggcac acttattagc gcggtactgc ttggtccggt gatcggaatg
     7621 ctgaccggct tactgaccaa tttactctgg ggattgctga ccgacccgat cgccgccgca
     7681 tttgcacccg tcgcgatggt catcggcctg gtcgccggtt ggctggcgcg agcgggttgg
     7741 tttcgcaccc tgccaaaagt cgttgtcagc ggcgtgatta ttacgcttgc ggtaacggtc
     7801 gtcgctgtac cgctgcgtac tgcgctgttc ggcggtgtca ccggtagcgg agccgatttg
     7861 ttcgttgcct ggatgcactc gatggggcaa aacctggtgg agtcggtggc gattacggtg
     7921 ataggcgcga acctggtcga taaaattctt acagcggtga ttgtctggct tttactgcgc
     7981 cagctgccga ttcgcaccac gcgccatttt ccggcaatgg ctgccgtgcg ctaaatgcat
     8041 ccatttacct cattaacact gtgggcattg gcggcctgca ccacgcttat cctgcccgcg
     8101 cagaccattt tgccgatcta tagcgcggcg accttttttt gtcttattgc gctaaaggcc
     8161 acgcggcggc gggcaaaata tgtcgcctgg ctgatgttct cgctgggagc cgggctgtgg
     8221 ctggtacacg gtggctggct aacagaatgg ctaagcggca cgccgcgttc gccggagcgt
     8281 tggacgcatg ctattacgct ctggttgcgg attctggcga ttgtttcaac ctcacagttg
     8341 tggatgcagt acgtccccgt gcaatgtttt atccgcgctc tgtttgcttc tcgcctgccg
     8401 ccaggcgtcg cttatctgtt tgccggaccg ttattggtcg ttgagcagtt aaaacggcaa
     8461 ttagcgatta ttcatgaagc gcagcgtgcc cgcggcgtgc cgctggatga gggctggtat
     8521 cagcgtttac gtgcgatgcc cgcgttaatt attctgctaa cacacaatgc gcttaacgat
     8581 ctggctgtcc gtggtgcggc gctggatatg cgggcatttc ggatccacaa ccgacgaaca
     8641 acgttgtggg caccagccga tagcacccta caacgcgtag cgcgttacac catgatcttg
     8701 ctgatgctaa cagaattcgg agcgtggata tggttacgtt agaacagttc cgttattgcc
     8761 cgacacactc aacgcatcct ccgttctgtt atgactttca ctatgtcaaa ccggggatgg
     8821 tcgccatctt tggtgacaac ggcagtggca aaagtacgct ggcacagctg atggcgggct
     8881 ggtatccgga ctttctgcca ggagagataa caggcacggg gacgctcctc ggtacgccca
     8941 ttgggcatct cccgcttaat gaacaatccg ccaccatcca gctcgtccag caatcgccct
     9001 atttgcagct ctccggctgt acctttagcg tggaagaaga agttgccttt gggccagaaa
     9061 atctgtgtct tgctgaaaca gagattatgg cgcggattga tgccgccctg actctgaccg
     9121 agtgccagcc gttgcgccat cgtcacccgg caacactctc cggtggagag acacaacgcg
     9181 tggtcatcgc ctgcgccatc gcgatgcagc caaagctctt aattcttgat gaagccttta
     9241 gtcgcctgac gccgcaggcc agcgaaatgc tgttgcagcg cttacaacac tgggcgcttg
     9301 aacgtggttc gctgattatt ctttttgaac gtcatcgcac acctttcctg aactactgcc
     9361 agcaagcgtg gcaattgcaa aacggagcgc tccagccact atgttaacgt taaatcagat
     9421 atcttatcgc tggcctagcg cagcaacgga ttgtctttgc gacatttctc tgcaactcaa
     9481 acaaggtgaa tggttggcgc tgaccggcga caatggcgcg ggaaaatcga cgctgttacg
     9541 agtaatggca ggtcttctta cgcccacagc cggtacggtt atgctgcaac aacaggcgat
     9601 gaaaaacctg aaaaaccgcc agcgcgcggc aaaggttggc gtactgtttc aggaagcgga
     9661 aaaccaactt tttcacagca ccgtcgctga cgagatcgca tttggtttga aactgcaaaa
     9721 atgcccggcg gatgaaatca ctcaacgtac ccatgctgcc ctgcaatgtt gtcagttagc
     9781 agacactgcc aactcgcacc cgttggatct gcatagtgcg cagcgacgga tggtcgccgt
     9841 cgcctgcctg gaagcactct ctccgccact attactgctg gacgagccga gtcgggattt
     9901 tgacgaaaat tggttgagcg tgtttgaaag ctggctggag aagtgccgcc agcgaggcac
     9961 gagcgtggta gcaatcagtc atgacgccgc atttacccgg cgtcatttct ctcgtgtggt
    10021 gcgactggaa gatggtgtaa tcaggaacgt taatccacca gacgatattc acccgtagcg
    10081 gtcatcatta aggtcaaatt tgcaggcaga ctctcttcca gcacccggcg aacattcggc
    10141 ccgtcggtac ggtcataaaa ggcttctgca tctgccagcg atacccctcc cgccagcttg
    10201 cgccccacca gacgctcccg caacgcagta gctggcgcgt tgataaagat cgagaaatca
    10261 caaaattgcg caagctggca ccacttctcg tcgtccagca acaaccagtt tccttcgacg
    10321 atgacgagtg gtgcggtaac gtgcaacgca tcttcaacag gatcatgctt ttgtcgatcg
    10381 tactgcggcc acgtacaatc cccttccacc acctggcgca gattttccgc cagtttcgca
    10441 acgtcaaatg tctctggtgc gcctttgaag gggcgcaatt gatgcgcgtc cagccagcta
    10501 ttgtaatggt gaaaaccatc catcgggagc gtctgaatag cgggcagctc cgggtcttgt
    10561 tgcacgagat attcccagaa ggtggttagg gtagatttac ccgtccccgg cggagcgcaa
    10621 agaaaaacca ccgtacgccg ctgcggattt accgtctgta aggccgccag catacgcagc
    10681 aacggtttat gcacgcgttc tatctcttca tcatggtact gcgcttgcac gttcagccca
    10741 ttcaccgtta attcaatttt cacggcgtta attcctttaa ggtggtcgtt actgccagcg
    10801 tcagcgccgt tttgaccatt ccgctaaaac gcgcc
//
LOCUS       DAZOZY010000137        10812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZOZY010000137 DAZOZY010000000
VERSION     DAZOZY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.137"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHURQ_003014"
     CDS             complement(<1..696)
                     /locus_tag="ACHURQ_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR0824736.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHURQ_003015"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHURQ_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR0824737.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHURQ_003016"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHURQ_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR0824738.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHURQ_003017"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHURQ_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR0824739.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHURQ_003018"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHURQ_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR0824740.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHURQ_003019"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHURQ_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR0824741.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHURQ_003020"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHURQ_003020"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR0824742.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHURQ_003021"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHURQ_003021"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR0824743.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHURQ_003022"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHURQ_003022"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR0824744.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHURQ_003023"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHURQ_003023"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR0824745.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHURQ_003024"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHURQ_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR0824746.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHURQ_003025"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHURQ_003025"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR0824747.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHURQ_003026"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHURQ_003026"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR0824748.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHURQ_003027"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHURQ_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR0824749.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         2715 a         2661 c         2878 g         2558 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 tctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcaagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa ac
//
LOCUS       DAZOZY010000138        10687 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZOZY010000138 DAZOZY010000000
VERSION     DAZOZY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.138"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHURQ_003028"
     CDS             complement(<1..784)
                     /locus_tag="ACHURQ_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR0824750.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHURQ_003029"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHURQ_003029"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR0824751.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHURQ_003030"
     CDS             1860..2747
                     /locus_tag="ACHURQ_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR0824752.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHURQ_003031"
     CDS             2780..3790
                     /locus_tag="ACHURQ_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR0824753.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHURQ_003032"
     CDS             3860..5293
                     /locus_tag="ACHURQ_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0824754.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHURQ_003033"
     CDS             5392..6741
                     /locus_tag="ACHURQ_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0824755.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHURQ_003034"
     CDS             6731..7618
                     /locus_tag="ACHURQ_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR0824756.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHURQ_003035"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHURQ_003035"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR0824757.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHURQ_003036"
     CDS             8352..9224
                     /locus_tag="ACHURQ_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR0824758.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHURQ_003037"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHURQ_003037"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR0824759.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHURQ_003038"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHURQ_003038"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR0824760.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2531 c         2855 g         2839 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggtc
//
LOCUS       DAZOZY010000139        10663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZOZY010000139 DAZOZY010000000
VERSION     DAZOZY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.139"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            319..894
                     /gene="ssuE"
                     /locus_tag="ACHURQ_003039"
     CDS             319..894
                     /gene="ssuE"
                     /locus_tag="ACHURQ_003039"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR0824761.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            887..1846
                     /gene="ssuA"
                     /locus_tag="ACHURQ_003040"
     CDS             887..1846
                     /gene="ssuA"
                     /locus_tag="ACHURQ_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR0824762.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1843..2990
                     /gene="ssuD"
                     /locus_tag="ACHURQ_003041"
                     /pseudo
     CDS             1843..2990
                     /gene="ssuD"
                     /locus_tag="ACHURQ_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            3002..3793
                     /gene="ssuC"
                     /locus_tag="ACHURQ_003042"
     CDS             3002..3793
                     /gene="ssuC"
                     /locus_tag="ACHURQ_003042"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR0824763.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3790..4557
                     /gene="ssuB"
                     /locus_tag="ACHURQ_003043"
     CDS             3790..4557
                     /gene="ssuB"
                     /locus_tag="ACHURQ_003043"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR0824764.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4764..7376)
                     /gene="pepN"
                     /locus_tag="ACHURQ_003044"
     CDS             complement(4764..7376)
                     /gene="pepN"
                     /locus_tag="ACHURQ_003044"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR0824765.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7642..8844
                     /gene="pncB"
                     /locus_tag="ACHURQ_003045"
     CDS             7642..8844
                     /gene="pncB"
                     /locus_tag="ACHURQ_003045"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR0824766.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9014..10414
                     /gene="asnS"
                     /locus_tag="ACHURQ_003046"
     CDS             9014..10414
                     /gene="asnS"
                     /locus_tag="ACHURQ_003046"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR0824767.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2438 a         2723 c         2845 g         2657 t
ORIGIN      
        1 ctttaaatga cattagactt aataataaca ataattgacc gccatttaaa tcaagcatac
       61 taaatccaat aagtgacaaa tctttaattt atagatttca tgaatattta aatattaact
      121 gaacgagtaa ttcattgatt caacatctaa tgactatcca acaaataaat atgcgtgata
      181 tataaagccc tttctttagt ttattttcag aaaaagatac acaaaattta tatttggcat
      241 tttcttgtct ctccgacaga ctgaccgaat tgttatcaat gttaacaaaa aaagaacaat
      301 tggttataag gagagagtat gcgtgtcatc accctggcgg gtagtcctcg ctttccttct
      361 cgctccagct ccttgctgga atatgcgcgg gaaaaactaa atggcctgga tgtagaggtt
      421 tatcactgga atctgcaaaa cttcgccccg gaagatctgc tttacgctcg tttcgatagt
      481 ccggcactca agaccttcac cgaacagctg caacaggctg atggactgat agtcgctaca
      541 cctgtgtata aagccgccta ttccggcgcg ctgaaaaccc tgctcgacct gctgccagag
      601 cgcgctttgc aaggcaaagt ggtgctaccg ctggcgacgg gcggtacggt ggctcatctg
      661 ctggcggtcg attatgccct taagccagtt ttaagtgcga tgaaagccca ggagctcctg
      721 cacggcgtgt ttgccgatga ctcacaagtt attgattacc atcacaaacc ccagttcacg
      781 ccaaatctgc aaacccgtct tgataccgcg ctagaaactt tctggcaggc attgcaccgc
      841 cgcgatgttc aggttcctga ccttctgtct ctgcgaggta atgtccatgc gtaacatcat
      901 taaactggcg ctggcgggat tgcttagcgt ctctactttt gcggttgctg cagaatcctc
      961 gcctgaagcg ttacgtatag gctatcagaa aggcagtatt ggtatggtat tggcaaaaag
     1021 ccaccagtta ctggaaaaac gctatccgca atcaaaaatc tcctgggtgg agttccccgc
     1081 gggtccgcaa atgttggaag cgttaaacgt tggcagtatt gatctcggca gtaccgggga
     1141 tattccgcca atctttgccc aggctgccgg ggctgatttg atatatgtgg gcgtcgagcc
     1201 accgaagccc aaagccgaag tgattctggt ggcagaaaac agcccgatca aaaccgtagc
     1261 cgatcttaaa ggtcacaaag ttgcctttca gaaaggttcc agttcacaca accttttact
     1321 gcgtgcactg cgtcaggccg gacttaagtt tactgacatc cagcccactt atctgacgcc
     1381 agctgatgcc cgcgccgcgt tccagcaagg taacgttgac gcctgggcta tctgggatcc
     1441 ctactactcc gctgcgttat tacagggcgg cgtgcgggtg ctgaaagacg gcaccgatct
     1501 caatcaaact ggatcgtttt atctggcagc tcgcccctat gcagaaaaaa acggcgcttt
     1561 tattcagggc gtactggcaa cctttagtga ggccgatgcg ttaacccgca gccagcgcga
     1621 gcaaagcatc gctttactgg caaaaacgat gggcttaccg gcaccggtga ttgcctcgta
     1681 tctggatcat cgtcctccca ccaccatcaa accggtgagc gctgaagttg ccgccttaca
     1741 gcagcaaacg gcagatctgt tttatgaaaa ccgtctggtg tcgaaaaaag ttgatattcg
     1801 ccagcgcatc tggcagccca ctcacctgga aggaaaacaa ttatgagtct gaatatgttc
     1861 tggtttttac cgacccatgg tgacgggcat tatcttggaa cggaagaagg ttcacgcccg
     1921 gttgatcacg gctatctgca acaaattgcg caagcggcgg atcgtctggg ctataccggt
     1981 gtgctgatcc caacggggcg atcgtgtgaa gatgcgtggc tggtggcggc gtcgatgatc
     2041 ccggtgacgc agcggctgaa gtttcttgtc gccctgcgtc ccagcgtaac ctcacctacc
     2101 gttgccgctc gccaggccgc cacgcttgac cgtctctcaa atggtcgtgc gttgtttaat
     2161 ctggtcacag gcagcgatcc acaagagttg gcaggcgacg gcgtgttcct tgatcatagc
     2221 gagcgctacg aagcctcggc ggaatttacc caggtctggc ggggcgctta ttgctggggg
     2281 aaagcgtcga tttcaacggt aaacatattc atgtgcgggg agcaaaactg ctcttcccgc
     2341 cgattcaaca gccgtatcct ccgctttact ttggcggatc gtcagatgtc gcccaggagc
     2401 tggcggcaga acaagttgat ctctacctca cctggggcga accgccagag ctggtaaaag
     2461 agaagatcga acaagtgcgg gcgaaagctg ccgcgcatgg acgcaaaatt cgtttcggta
     2521 ttcgtctgca tgtgattgtt cgtgaaacta acgacgaagc gtggcaggcc gccgagcggt
     2581 taatctcgca tcttgatgat gaaactatcg ccaaagcaca ggccgcattc gcccggacgg
     2641 attccgtagg acaacagcga atggcggcgc tacataacgg caagcgcgac aatctggaga
     2701 tcagccccaa tttatgggcg ggcgttggct tagtgcgcgg cggtgccgga acggcgctgg
     2761 tgggcgatgg tcctacggtc gccgcgcgaa tcaacgaata cgccgcgctt ggcatcgaca
     2821 gctttgtgct ttcgggctac ccgcatctgg aagaagcgta tcgggttggc gagttgctgt
     2881 tcccacatct ggatgtcgcc attccggaaa ttccccagcc gcagccgctg aatccgcaag
     2941 gcgaagcggt ggcgaatgat tttatccccc gtaaagtcgc gcaaagctaa ggaacacgat
     3001 gatggcaacg ccagtgaaga agtggttatt gcgcgttgcc ccctggtttt taccggtggg
     3061 catcgtggcg gtgtggcaac tggcctcctc ggttggctgg ctgtcgacgc gtattttgcc
     3121 ttcaccggaa ggagtggtga cggcgttctg gacgctctcc gccagcggcg aattgtggca
     3181 gcatctggcg atcagttcct ggcgggcgct gattggcttt tcaattggcg gatcgctggg
     3241 cttgattctg ggactgatta gcgggctgtc acgctgggga gaacggctgc tggatacctc
     3301 aattcagatg ttgcgcaacg taccgcatct ggcgctgatc ccgctggtga ttttgtggtt
     3361 tggcattgat gaatccgcaa aaatctttct ggtggcgctc ggcacgctat tccccattta
     3421 tatcaacacc tggcatggca tccgtaatat cgatcgcggt ctggtggaaa tggcgcgtag
     3481 ctatggatta tccggcatac cgctgtttat ccatgtgatc ctgcctggtg ccctgccctc
     3541 aattatggtc ggcgtgcgtt ttgcgttggg cctgatgtgg ctgacgctga ttgttgccga
     3601 aaccatttct gccaattcag gcattggtta tctggcgatg aatgcgcggg agtttttgca
     3661 aacggacgtg gtggtagtcg ctattattct ttacgccctg ctcggcaaac ttgctgacgt
     3721 cagcgcgcag ctgttagagc gcctctggtt gcgctggaac ccggcttatc atttgaagga
     3781 ggccacggta tgaatactgc tcgtctgaac cagggcacac cattgttgct caatgcagta
     3841 agcaaacatt acgcggaaaa tatcgtcctg aaccaactgg atttacatat tccggcaggt
     3901 cagtttgtgg cggtggtggg ccgcagcggt ggtggcaaaa gtaccctgct gcgcctgctg
     3961 gcaggtctgg aaacgccaac cgcaggcgat gtgttagcgg gcaccacacc gctggctgag
     4021 attcaggaag acacgcgaat gatgtttcag gatgcgcgtc tgctgccgtg gaaatcggtg
     4081 attgataacg ttgggttagg ccttaaaggc cagtggcgcg atgccgcgcg tcgggcgttg
     4141 gctgcagtag gactggagaa tcgcgccggg gaatggcctg ccgcactttc tggcgggcag
     4201 aaacagcgtg tggcgctggc aagagcgttg attcatcgac cgggtttatt gttgcttgat
     4261 gaaccgctcg gggcactgga cgccttaacg cgactcgaga tgcaggattt gattgtgtca
     4321 ctttggcagc agcacggctt taccgtactg ttggtgacgc atgatgtgag cgaagcggta
     4381 gcaatggctg accgggtgtt gttaattgaa gagggaaaaa ttggtctgga tttgacagtg
     4441 gatatccccc gtccgcgtcg attagggtcg gtgcgattag cggagctgga agcggaagta
     4501 ttgcaacggg taatgcggcg tgggcattct gaacaactga ttcggcgtca tggttaatat
     4561 gccggatgtg gcgcacaaaa ccagcatttt caacggactt gtaggcctga taagacacgt
     4621 caagcatcgc atcaggcagt tgggcacggc tgccggatgc ggcgtgaacg ccttatccag
     4681 cctacaaaac cagcaatttc aacggagttg taggcctgat aagacgcgtc aagcgtcgca
     4741 tcaggcattt tggctcaaaa gtatcaagcc agtgctttgg tgatcttctc gtacagatcg
     4801 ccagagagat tttccagccc tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt
     4861 ttggcatcat aacgtttcag gcgaatcagc ggttcaatca gacgtgaagc tacctgcggg
     4921 ttacggctgt tgaggtcggt aagcatttcc accaggaact ggtaaccgct gccatcttcg
     4981 gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg aatacggttc
     5041 gggttgctca tggtaaatga gcgatgctgc aacagaccac gcaccgtctc cagcacattc
     5101 gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc gtcctgatgc
     5161 cacttatcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc
     5221 gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg cttgctcacc
     5281 agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc atttcgcaga
     5341 gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa
     5401 atagccagca gctcatccgc cagttcagtc gccagagtac gagtgagtgc ttcgcgtact
     5461 tcggcaatag caatcgggtc gatgatatcg aacagttcag ccatttcatt gacagaaggc
     5521 agcgtcagga tttctgccgc cagcgccgga tcaatcttct catcgagcag caccgcgcgg
     5581 aaagcgtcag ccacatgtac cggcagagac agcggctgcc cttgctgatg acgggcgacg
     5641 ttcagcttga tgtaggttgc cagcaaactt tgcgccgcat cccaacggga gaaatcatta
     5701 cgcgcatgac gcatcaggaa ggtcagttgc tgatcactcc acttatattc cagtttcact
     5761 ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt atcaaaaaca
     5821 aaggtctgtt ccgcctgggt gacgttcagc acggaattca ccggatgacc gcctttctgc
     5881 aacgggatca ctttgccttc gttgtcatac aattcgatgg caaacggaat atgcagcggc
     5941 tgtttttctg cctgatccgg cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc
     6001 tcagtttccg gattgtagtc gtctttgacg gtcacaatcg gcgtaccgga ctggctgtac
     6061 caacggcgga aatgggagag atcaacattc gacgcatctt ccatcgcctg cacaaagtcg
     6121 tcacaggtcg ctgcgctacc atcatgacgc tcgaaataga gttgcatccc tttctggaag
     6181 ttttcttcgc ccagcagggt gtggatcata cggatcactt ccgcgccctt ctcgtaaacg
     6241 gtcagggtgt agaagttgtt catctcaatg accatgtccg ggcggattgg gtgcgccatc
     6301 gggctggcgt cttccgcaaa ctgcaatccg cgcatggtgc gcacattatt gatgcggtta
     6361 actgcgcggg aaccaaggtc agagctgaat tcctgatcgc ggaagacggt taaaccttct
     6421 ttcaggctga gctggaacca gtcgcggcag gtcactcggt taccggtcca gttatggaaa
     6481 tattcatggc cgataacgcg ttcaatatcg aggtaatctt tgtcggtggc ggtgtcggtg
     6541 cgggccagca catatttgga gttaaagata ttcagaccct tattctccat tgcgcccata
     6601 ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag gccgaagcgt
     6661 tcttcatccc atttcattga gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg
     6721 ttgccgcgat cgacgtacag ctccagcgcc acttcgcgac cagaacgcgt ggtaaaggtg
     6781 tcgcgcagta catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac
     6841 gggtcctgcc actgtaccca atggcgtccg ttttccagtt caccttgcgc aacgcggtta
     6901 ccgttggaaa gcaggaaggg atatttggtt ttatcggcaa taattttggt ggtaaaacgc
     6961 gccagcacgt ccgggcggtc gagataatac gtaatatggc ggaaaccttc ggcttcacac
     7021 tgggtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgcggtatt cgccgccggg
     7081 cttatttcat taacgatctt aagcgtaaaa tgctccggca aattactgat gaccagtgcg
     7141 ccctcttctt ctttccaggc ggtccacggc tcatcattaa tatgaacaga aaccagtttg
     7201 aggtcttcgc cgttgagacg aagtggagca tctgatgcac catgacggac agcctggctg
     7261 accgcggtaa cgaccgtctt ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc
     7321 tggtaatccg gcgcacgata atcgtgacgg tatttggctt gtggctgttg agtcataaat
     7381 aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc
     7441 tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt ttataacata
     7501 caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt tcaataaatt
     7561 gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa cgctcctgta
     7621 cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg
     7681 gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcacta ttacgatgtg
     7741 catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta tgccgatgct
     7801 attcgtgaac agattcaggc gatgcagcac ctgcgcctgc aggatgatga atatcagtgg
     7861 ctctctgccc tgcctttctt taaggccgac tatcttaact ggttacgcga gttccgcttt
     7921 aacccggaac aagtcaccgt gtccaacgat aatggcaagc tggatattcg cttaagcggt
     7981 ccgtggcgtg aagtcatcct ctgggaagtt cctttgctgg cggttatcag tgaaatggta
     8041 catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa
     8101 ttagtcgact tctcggcgtt aaccgccggt cttgatatgt cgcgcttcca tctgatggat
     8161 tttggcaccc gtcgccgttt ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa
     8221 caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct ttccctcacg
     8281 ccgatgggaa cacaggcaca cgaatggttc caggcacatc agcaaatcag cccggatcta
     8341 gccaacagcc agcgagctgc acttgctgcc tggctggaag agtatcccga ccaacttggc
     8401 attgcattaa ccgactgcat cactatggat gctttcctgc gtgatttcgg tgtcgagttc
     8461 gctagtcggt atcagggcct gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa
     8521 gccattgcac attatgaaaa gctgggaatt gatccacaga gtaaaacgct ggttttctct
     8581 gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc ccgcgtgcaa
     8641 ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat
     8701 attgtgatta agttggtaga gtgtaacggt aaaccggtgg cgaaactttc tgacagccct
     8761 ggcaaaacta tctgccatga taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt
     8821 ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc tttttttacc
     8881 tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg cagatgccag
     8941 gtaacatagg tatccccccc atttacgggt ggacaagtgt ttattttttc cgactattaa
     9001 cagagagaat attatgagcg ttgtgcctgt agccgacgta ctccagggcc gcgtagccgt
     9061 tgacagcgaa gtcaccgtac gcggatgggt acgtacccgc cgagattcaa aagctggcat
     9121 ctccttcctc gccgtttatg acggttcctg ctttgatcct gtacaggctg tcatcaataa
     9181 ttctctgccc aattacaatg aagacgtcct gcgtctgacc accggctgct cggtcattgt
     9241 gacgggtaaa gtcgtggcat cgccgggcca ggggcaacaa tttgaaattc aggccagcaa
     9301 ggttgaagtt gctggttggg ttgaagatcc agacacttac ccgatggcgg caaaacgcca
     9361 cagcattgag tatctgcgtg aagtcgctca cctgcgtccg cgcacaaacc tgattggtgc
     9421 cgtcgcgcgc gttcgccata cgctggcgca ggcgctgcat cgcttcttta acgagcaggg
     9481 attcttctgg gtttcaacgc cactgattac cgcatctgat accgaaggtg caggcgaaat
     9541 gttccgcgtt tctacgctgg atctggaaaa cctgccgcgt aacgatcagg gcaaagtgga
     9601 tttcgacaaa gacttctttg gtaaagagtc tttcctgacc gtatctggcc agttgaacgg
     9661 cgaaacctac gcttgcgcat tatccaaaat ttataccttc ggcccgactt tccgtgctga
     9721 aaactccaac accagccgtc acctggcgga attctggatg ctggagccgg aagtggcgtt
     9781 tgctaacctg aacgatatcg cgggtctggc tgaagccatg ctgaaatatg tcttcaaagc
     9841 ggttctcgaa gaacgcgcag acgacatgaa attcttcgct gaacgcgtag ataaagatgc
     9901 cgtttcacgt ctggaacgct tcattgaggc cgatttcgcg caggtggatt acaccgacgc
     9961 agtgaccatt ctcgaaaact gcggcaggaa gtttgaaaac ccggtttact ggggcgtcga
    10021 tctctcttct gagcatgagc gttatctggc ggaagaacac tttaaagcac cggtagtggt
    10081 taaaaactat ccgaaagata ttaaagcgtt ctatatgcgc ctcaacgaag acggtaaaac
    10141 cgttgcggct atggacgttc tggctccggg catcggtgag atcattggtg gctcccagcg
    10201 tgaagaacgt ctggacgttc tggacgagcg tatgctggaa atgggcctga ataaagaaga
    10261 ttactggtgg tatcgcgatc tgcgtcgcta cggtactgtt ccgcattcag gtttcggtct
    10321 tggttttgaa cgcctgattg cttacgtaac tggcgtgcaa aacgtgcgtg atgtgattcc
    10381 gttcccacgt actccgcgca acgccagctt ctgattttct tcctcaaaga gccagcgcct
    10441 gctggctctt ttttatcctt tctgtcaagt tatctgtttg ttaagtcaag caatctattt
    10501 gcaaccccgc cataacgatc atcctgttac ggaatattac attgcaacat ttacgcacaa
    10561 aaaataaccc gcattcttat tgcggattag ttttttctta gctgatagca caattttcat
    10621 actatttttt ggcattctgg atgtctgaaa gaggattttg tgc
//
LOCUS       DAZOZY010000140        10549 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZOZY010000140 DAZOZY010000000
VERSION     DAZOZY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10549
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.140"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(72..1100)
                     /gene="malI"
                     /locus_tag="ACHURQ_003047"
     CDS             complement(72..1100)
                     /gene="malI"
                     /locus_tag="ACHURQ_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR0824768.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1275..2867
                     /gene="malX"
                     /locus_tag="ACHURQ_003048"
     CDS             1275..2867
                     /gene="malX"
                     /locus_tag="ACHURQ_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR0824769.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2877..4049
                     /gene="malY"
                     /locus_tag="ACHURQ_003049"
     CDS             2877..4049
                     /gene="malY"
                     /locus_tag="ACHURQ_003049"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR0824770.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4153..5154
                     /gene="add"
                     /locus_tag="ACHURQ_003050"
     CDS             4153..5154
                     /gene="add"
                     /locus_tag="ACHURQ_003050"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR0824771.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5190..6230)
                     /locus_tag="ACHURQ_003051"
                     /pseudo
     CDS             complement(5190..6230)
                     /locus_tag="ACHURQ_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6474..6599
                     /gene="blr"
                     /locus_tag="ACHURQ_003052"
     CDS             6474..6599
                     /gene="blr"
                     /locus_tag="ACHURQ_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR0824772.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6864..7079
                     /gene="ydgT"
                     /locus_tag="ACHURQ_003053"
     CDS             6864..7079
                     /gene="ydgT"
                     /locus_tag="ACHURQ_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR0824773.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7165..7605
                     /locus_tag="ACHURQ_003054"
     CDS             7165..7605
                     /locus_tag="ACHURQ_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR0824774.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7682..8263
                     /gene="rsxA"
                     /locus_tag="ACHURQ_003055"
     CDS             7682..8263
                     /gene="rsxA"
                     /locus_tag="ACHURQ_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR0824775.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8263..8841
                     /gene="rsxB"
                     /locus_tag="ACHURQ_003056"
     CDS             8263..8841
                     /gene="rsxB"
                     /locus_tag="ACHURQ_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR0824776.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8834..>10549
                     /gene="rsxC"
                     /locus_tag="ACHURQ_003057"
     CDS             8834..>10549
                     /gene="rsxC"
                     /locus_tag="ACHURQ_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR0824777.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLE"
BASE COUNT         2411 a         2728 c         2700 g         2710 t
ORIGIN      
        1 cctgtgtgcc acatttttta tcattaacct tgaggaaagg acactaccag gtatagcaga
       61 taaagagagg attatttcgc tgcaatgagc cgggcgggaa taataagatt gcgtgaatgc
      121 gtctcttcat gggtgatttt ttgcatcatg cgatccgcaa gtgtggtacc aagttcccgc
      181 gcaggcgtgc tggcccaggt aacgggtata tcatcaagtg tggttggtgt cgcatcggta
      241 aatgccgcca gcgaaacttg ttgctcaaag taacgatcga ctccgctttc cccgctttgc
      301 cgccctgctt tcagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt atagcaaacc
      361 acagcactga tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc ggcttgcttc
      421 tggctggatg tgcactccag cacccaatcg ctgtgaaacg gcaggccaaa ttttagtaga
      481 gttgcacaat agcctcccac ccgttctgcc cgggttaatg aggaactttg ccctcccagc
      541 caggcgatcc gctgatgccc attgcgaatg agatgctccg tcaacaactg tgcagcctgc
      601 atgttgtccg ggcgaaccgt atcaacatca tcaagataac tggcacggga agcgaaaatc
      661 accgggatag ctttttcttc tgccatccgt cgcaggtcat cgctgcttcc tgcagccccg
      721 gcaattacca caccatcgac accctgattc agtaacagtg aaaaccgctg tgccagctgc
      781 tcgccgtctt taccgccgtg aagcaaaaaa accatccgtc cctgcgcttc cagagcttcc
      841 gtcaatccgg ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac gatcaaacca
      901 atgacgccgc tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac aaatcccagc
      961 tcttcaatgg cggcgttaac gcgttctcct gtggcggtag agattcgccc tttgccacta
     1021 agcaccagcg aaacggtact taccgacacg cccgcagcca gcgcaacatc atgaatggtt
     1081 atttttttgg cggtagccat gcagatgacc tactccctga ttatgtgaca gataaaacgt
     1141 tttacctttt atttatctta tacccgctat tatcgttgcg taatgtgatt tatgcttcac
     1201 taaaatttga taaaacgttt tatcttctcg cgcaatttac tgaatccaga ttgttctcta
     1261 cgaggagtcg ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg gagttcttcc
     1321 agcagttagg caaaaccttc atgttacccg tggcattatt gtcgttctgc ggcattatgc
     1381 tcggcattgg tagttctctt agtagccacg atgtcatcac cctgatcccg gtcctgggca
     1441 accccgtgtt gcaggctatc tttacctgga tgagtaagat tggctcgttt gcttttagtt
     1501 tcctgccagt catgttctgt atcgccatcc cgctgggtct ggcacgcgaa aacaaaggcg
     1561 tagcggcatt cgctggcttc gtcggttatg cggtaatgaa cctcgcggta aacttctggt
     1621 tgaccaataa aggcattctg ccaaccacgg acgccgcggt tctgaaagcc aataacatcc
     1681 agagcattct tgggatccag tcgatcgaca ccgggatcct cggtgcggtg atcgccggta
     1741 ttatcgtctg gatgctgcat gagcgattcc ataatatccg cctgccggat gcgcttgcat
     1801 tcttcggcgg tacgcgcttc gtaccaatca tctcctcgct ggtgatgggc cttgtcggcc
     1861 tggtgattcc attagtctgg ccgattttcg cgatgggtat tagcggcttg ggccatatga
     1921 taaacagcgc gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt ctgctgttgc
     1981 cgtttggtct gcatcacatt ctggtggcat taattcgctt taccgacgca ggcggcacgc
     2041 aggaagtctg cggtcaaacc gtcagcggcg cattgaccat cttccaggcg caattgagtt
     2101 gcccgaccac tcacggtttt tctgaaagcg ccacgcgttt cctttcgcaa ggcaaaatgc
     2161 ctgcgtttct cggcggtctg ccaggtgcag cgttagcgat gtatcactgc gcgcgcccgg
     2221 aaaatcgcca taaaattaaa gggctgctga tttctggcct gatcgcctgt gtcgttggcg
     2281 gcacgaccga accgctggaa ttcctgttcc tgttcgtggc gccagttctg tatgtcatcc
     2341 acgcgctgtt aaccggcctc ggcttcactg tcatgtctgt gctcggcgtc accatcggta
     2401 ataccgacgg caatatcatc gacttcgtgg tgttcggtat tttgcatggt ctgtcaacca
     2461 agtggtacat ggtgccagtg gtggcggcaa tctggtttgt cgtttactac gtcatcttcc
     2521 gtttcgctat cacccgcttc aatctgaaaa ccccggggcg cgatagcgaa gtggccagtt
     2581 caatcgaaaa agccgttgcc ggtgcgccgg gtaaatcagg ttacaacgtt ccggcaatcc
     2641 tcgaagcctt aggcggtgcc gacaatattg ttagcctcga taactgcatt acccgtctgc
     2701 gtttgtctgt gaaagatatg tcgcttgtta atgtgcaggc actgaaggac aatcgggcaa
     2761 ttggcgtagt acaacttaat caacataacc tgcaggttgt tatcgggcca caagttcagt
     2821 cagtaaaaga tgaaatggcc ggtctgatgc atactgtcca ggcataagga taagatatgt
     2881 tcgatttttc aaaggtcgtg gatcgtcatg gcacatggtg tacacagtgg gattatgtcg
     2941 ctgaccgttt cggcactgct gacctgttac cgttcacgat ttcagacatg gattttgcca
     3001 ctgccccctg cattatcgag gcgctgaatc agcgcctgat gcacggcgta tttggctaca
     3061 gccgctggaa aaacgatgag tttctcgcgg ctattgccca ctggttttcc acccagcatt
     3121 acaccgcaat tgatacgcag acggtggtgt atggcccttc tgtcatctat atggtttcag
     3181 aactgattcg tcagtggtct gaaacaggtg aaggcgtggt gatccacacg cccgcctatg
     3241 acgcatttta caaggccatt gaaggtaacc agcgcacagt aatgcccgtt gctttagaga
     3301 agcaggctga cggctggttt tgcgatatgg gcaagctgga agccgttttg gcgaaaccag
     3361 aatgtaaaat tatgctcctg tgtagcccac agaatcctac cgggaaagtg tggacgtgcg
     3421 atgagctgga gatcatggct gacctgtgcg agcgtcatga tgtgcgggtt atttccgatg
     3481 aaatccatat ggatatggtc tggggcgagc agccgcatat tccctggagt aatgtggcgc
     3541 ggggagactg ggcgttgctg acgtcgggat cgaaaagttt caatattccc gcgctgaccg
     3601 gtgcttacgg gattatagaa aatagcagca gccgtgatgc ctatttatcg gcactgaaag
     3661 gacgtgatgg gctttcttcc ccttcggtac tggcgttaac tgcccatatc gccgcctatc
     3721 agcaaggtgc accgtggctg gatgccttac gcgtctatct gaaagataac ctgacgtata
     3781 tctcagataa aatgaacgcc gcgtttcctg aactcaactg gcagatcccg caatccactt
     3841 atctggcctg gcttgatcta cgtccgttga atattgacga caacgcgttg caaaaagcgc
     3901 ttatcgagca agaaaaagtc gcgatcatgc cggggtatac ctatggtgaa gaaggtcgtg
     3961 gttttgtccg tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt gtggctggat
     4021 taataaacgc catccgcgct gttcgctaac cccaattgcg caacgtaaaa aatcgttgcg
     4081 caatcgtgga tttttaccct gctttgtttt tataatggtg cgcactttta tatccagaaa
     4141 aagagtgcga ccatgattga taccaccctg ccattaactg atatccatcg ccaccttgat
     4201 ggcaacattc gtccccagac cattcttgaa cttggccgcc agtataatat ctcgcttcct
     4261 gcacaatccc tggaaacact gattccccac gttcaggtca ttgccaacga acccgatctg
     4321 gtgagctttc tgaccaaact tgactggggc gttaaagttc tcgcctctct tgatgcctgc
     4381 cgccgcgtgg catttgaaaa cattgaagat gcagcccgta acggcctgca ctatgtcgag
     4441 ctgcgttttt caccaggcta catggcaatg gcacatcagc tgcctgtagc gggtgttgtc
     4501 gaagcggtga tcgatggcgt acgtgaaggt tgccgcacct ttggtgtgca ggcgaagctt
     4561 atcggcatta tgagccgaac cttcggcgaa gccgcctgtc agcaagagct ggaggccttt
     4621 ttagcccacc gtgaccagat taccgcactt gatttagctg gtgatgaact tggattcccg
     4681 ggaagtctgt tcctttctca cttcaaccgc gcgcgtgatg cgggctggca tattaccgtc
     4741 catgcaggcg aagctgccgg gccggaaagc atctggcagg cgattcgtga actgggtgcg
     4801 gagcgtattg gacatggcgt aaaagccatt gaagatcggg cgctgatgga ttttctcgcc
     4861 gagcaacaaa ttggtattga atcctgtctg acctccaata ttcagaccag caccgtagca
     4921 gagctggctg cacatccgct gaaaacgttc cttgagcatg gcattcgtgc cagcattaac
     4981 actgacgatc ccggcgtaca gggagtggat atcattcacg aatataccgt tgccgcgcca
     5041 gctgctgggt tatcccgtga gcaaatccgc caggcacaga ttaatggtct ggaaatggct
     5101 ttcctcagcg cagaggaaaa acgcgcactg cgagaaaaag tcgccgcgaa gtaacaaaaa
     5161 tggatggtgc aatttgcacc atccattttt cacgcaaggc acaacgtcgc gcgatgtttg
     5221 gcggattcga tgcccagctc aatcaactcc attacctgga ttgcctagct tgccggaacc
     5281 ggattttcac catcgccatt taacgcatca cgaatcgccg catagtaagc cggataattc
     5341 ccaggcaccg tcaacagcgt ttcttcgaca cgttcctcac cttccacgcg ggtaagtacg
     5401 ccatcacgca tatcgtagcc ccagtcttcc tgcggcagac gctcgccatt tttcagacgt
     5461 tcttcctgtg gatcgaggcc atatttcaca taactgcctc gggatccatg cacgatatac
     5521 cgtgctgact cagcggctgc cagcatggta ccgtgtaaaa tgactcgccg ctgtggatag
     5581 gacaagatgg cgtggaaata atcggtcgac tgcgctccgg gccgtaactg tgccaaatct
     5641 accgtcatgc tgaccggtaa accaaatagc gtaatggcct gatcaagaag atgcggtgct
     5701 aaatcgtacc agataccgct gcctgggccg ccctgttcac gccaacgatc gcgcacctgc
     5761 ggacggaagc ggtcaaaatg agactcaaag taagcaactt cacccagcac gccttccgcg
     5821 agcaaacctt ttagcgtcaa gaaatcgcta tcccaacgac ggttatggaa tacagacagc
     5881 acacgcccca ggctttttgc cagcgcatcc agctctcgcg cttgtgacag tgtcacggta
     5941 aagggtttat caacgaccac atgtttaccc gcctcaagcg ccgctttggc taacgggaaa
     6001 tgggtatcgt tgggtgtagg aatgacaatc aggtctatgt tgggatcgtt aaacagatgc
     6061 ttcggctcag agacaaccgt aaccgttggc cagtcggctt ttacttttgt ttcatcactg
     6121 ctggagatta ccgccagttc cagcccgggc gtgcccgcaa tcaggggcgc atggaaggtt
     6181 ttgctcgcat aaccataccc aatcaaccca acacggatgt tgtcgctcat gtcattgcct
     6241 ctcattgaag tatgatggct atttgacact atcctttacc cacgctcaac agtttaataa
     6301 cctgccagca ataagggatg ttgtttaact taagtcaaaa aaaatagcga attttccaac
     6361 gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa
     6421 gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc
     6481 gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg
     6541 cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag
     6601 ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat
     6661 taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg
     6721 cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt
     6781 attgtaataa cacaagcgtt cacttttggt gaaattttat catcactcta cccctttggt
     6841 attaattatt ttatggacct tttatgactg ttcaggacta cttattaaaa tttcgcaaaa
     6901 tcagttcact cgaaagtctg gaaaaactct acgaccatct taattacacc ctgacggacg
     6961 atcaggaact gatcaatatg tatcgtgctg ccgatcaccg tcgcgcagag ctggtttctg
     7021 gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt ctggcactat gtccaataaa
     7081 gaaagtagcg atcggttata aaagccttat aataacgccc ctaaaattag atttgcctgt
     7141 ttgcgtcgct attctggaga acctatgacc accacaacgc cacaacgtat tggaggctgg
     7201 ttgcttggcc cgttggcttg gcttttagtc gctctgttga gtacgacgct ggcgttgttg
     7261 ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa cgctcggcgg acaagcactg
     7321 acgacacaaa tcttatgggg cgtttctttt attaccgcta tcgccatgtg gtattacaca
     7381 ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg ttcctaaaca ctatattatc
     7441 tggctgctta tttccgtatt gctggcagta aaagcattcg ctttttcacc cgtggaagat
     7501 ggcatagccg ttcgccagtt gctgtttact ttgttggcaa ccgcattgat cgtcccctat
     7561 ttcaaacgct cgtcgcgggt aaaagcgacg tttgtgaatc cgtaataacc ttacagttaa
     7621 cctgttgtcg cctgctctgg attaacggat aataggcggc ttttttattt caggccgaaa
     7681 aatgactgac tacctgttac tgtttgtcgg aactgtactg gtcaataact ttgtactggt
     7741 caagtttctc ggtctctgtc cgtttatggg ggtttccaaa aaactggaaa ccgcgatggg
     7801 catggggctg gcaacaacgt ttgtgatgac gctggcgtct atttgcgcct ggcttatcga
     7861 tacgtggatt ttgatcccac ttaatctgat ttacctgcgc accctggcat ttattctggt
     7921 gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc aaaaccagcc cggtgcttta
     7981 ccgtctgctg gggatttttt tgccgcttat caccaccaac tgtgcagtgc tcggcgtggc
     8041 gttgctgaat atcaatctcg ggcacaattt cttgcagtcg gcgctgtacg gtttttccgc
     8101 cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc atccgcgaac gccttgctgt
     8161 ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg ttaattaccg caggtcttat
     8221 gtctctggcc tttatgggct ttaatggttt ggtgaagttg taatgaatgc tatctggatt
     8281 gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg ccattctggg ttatgcctcc
     8341 cgccgttttg cggtggaaga cgatccggtc gttgagaaaa ttgacgaaat cttaccgcag
     8401 agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct acgcggaagc catcagctgt
     8461 aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag ctgtgatgct aaaaattgcc
     8521 gagttgctta atgtcgagcc gcagccgctg gatggcgaag cgcaagagct aacgcctgcg
     8581 cggatggtgg cggttattga tgaaaataac tgtattggct gcactaaatg tattcaggct
     8641 tgtccggtag acgccatcgt tggcgctacc cgtgccatgc atacggtaat gagtgatctc
     8701 tgtacgggct gcaatttatg tgttgatccg tgcccgacgc actgcatctc gttgcaaccg
     8761 gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca ccattcccgt gcgtatcatt
     8821 cccgtggaac accatgctta agttattctc tgcattcaga aaaaataaaa tctgggattt
     8881 caacggcggc atccatccac cggagatgaa aacccagtcc aacggtacac ccctgcgcca
     8941 ggtaccactg gcgcagcgtt ttgttattcc actgaaacag catattggcg ctgaaggtga
     9001 gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag ccgcttaccc gtggtcgcgg
     9061 caaaatgctg cctgttcacg cgcccacctc gggtaccgtt acggctattg cgccccactc
     9121 tacggctcat ccttcagctt tagctgaatt aagcgtgatt attgatgccg atggtgaaga
     9181 ctgctggatc ccgcgcgacg gctgggccga ttatcgcact cgcagtcgcg aagagttaat
     9241 cgagcgcata catcagtttg gtgttgccgg gctgggcggt gcaggattcc cgacaggcgt
     9301 taaattgcag ggtggcggag ataagattga aacgttgatt atcaacgcgg ctgagtgcga
     9361 gccgtacatt accgccgatg accgtttgat gcaggattgc gcggctcagg tcgtagaggg
     9421 tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt cttatcggca ttgaagataa
     9481 caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg gactctaacg atatttctct
     9541 gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa caattaacct acattctgac
     9601 cgggaagcag gttccacatg gcgggcgttc atccgatatc ggcgtattaa tgcaaaacgt
     9661 cggcactgct tatgcagtga aacgtgccgt tattgatggc gagccgatta ccgagcgtgt
     9721 tgtaaccctg actggcgaag caatcgctcg cccgggcaac gtctgggcac ggctggggac
     9781 gccagtgcgt catttattga atgatgccgg attctgcccc tctgccgatc aaatggtgat
     9841 tatgggtggc ccgctaatgg gctttacctt gccatggctg gatgtcccgg tcgtaaagat
     9901 taccaactgt ctgttggctc cctctgccaa tgaacttggc gaaccacagg aagaacaaag
     9961 ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgcg gatcttttgc cgcaacagtt
    10021 gtactggttc agcaaaggtc agcaacacga taaagctacc acgcataaca ttgctgattg
    10081 cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat attcccctgg tgcaatattt
    10141 ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa gaaaagcgcg ccgcagaagc
    10201 caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc gaaaaagcgg ctcgccttga
    10261 acgacataag agcgcagccg ttcaacctgc agccaaagat aaagatgcga ttgctgccgc
    10321 tctggcgcgg gtgaaagaga aacaggccca ggctacacag cctattgtga ttaaagcggg
    10381 cgaacgcccg gataacagtg caattattgc agcacgggaa gcccgtaaag cgcaagccag
    10441 agcgaaacag gcagaactgc agcaaactaa cgacgcagca accgttgctg atccacgtaa
    10501 aactgccgtt gaagcagcta tcgcccgcgc caaagcgcgc aagctggaa
//
LOCUS       DAZOZY010000141        10455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZOZY010000141 DAZOZY010000000
VERSION     DAZOZY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.141"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHURQ_003058"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHURQ_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR0824778.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHURQ_003059"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHURQ_003059"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR0824779.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHURQ_003060"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHURQ_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR0824780.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHURQ_003061"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHURQ_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR0824781.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHURQ_003062"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHURQ_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHURQ_003063"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHURQ_003063"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0824782.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHURQ_003064"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHURQ_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR0824783.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHURQ_003065"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHURQ_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR0824784.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLVVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHURQ_003066"
     CDS             complement(9457..10149)
                     /locus_tag="ACHURQ_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR0824785.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2557 a         2522 c         2762 g         2614 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccacc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAZOZY010000142        10382 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZOZY010000142 DAZOZY010000000
VERSION     DAZOZY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.142"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            216..1898
                     /locus_tag="ACHURQ_003067"
     CDS             216..1898
                     /locus_tag="ACHURQ_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR0824786.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1950..3107
                     /locus_tag="ACHURQ_003068"
     CDS             1950..3107
                     /locus_tag="ACHURQ_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR0824787.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3073..3195)
                     /locus_tag="ACHURQ_003069"
     CDS             complement(3073..3195)
                     /locus_tag="ACHURQ_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824788.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            3404..5089
                     /locus_tag="ACHURQ_003070"
     CDS             3404..5089
                     /locus_tag="ACHURQ_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR0824789.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            5127..7499
                     /locus_tag="ACHURQ_003071"
     CDS             5127..7499
                     /locus_tag="ACHURQ_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR0824790.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7556..10339
                     /locus_tag="ACHURQ_003072"
     CDS             7556..10339
                     /locus_tag="ACHURQ_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR0824791.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3067 a         2285 c         2253 g         2777 t
ORIGIN      
        1 aaaatgcaat aatttattaa atatcaataa attagttgtt tatcggcgag aaattactta
       61 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
      121 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
      181 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
      241 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
      301 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gattttacgc
      361 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
      421 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
      481 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
      541 cgccgacgct cctttcatta atggatgaag gcgtacgttt tactaacggc tatgtggcac
      601 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
      661 gtgtctattc caataccgat gctcaggatg gtattccgct aacagaaact ttcttgcctg
      721 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
      781 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
      841 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
      901 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
      961 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
     1021 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctaccaa
     1081 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     1141 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     1201 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     1261 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     1321 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     1381 atgacaagct gatttccgca atggatttct acccaacagc tcttgatgca gccgatatca
     1441 gcattccaaa agaccttaag ctggatggtg tttccttgct gccctggttg caagataaga
     1501 aacaaggcgc gccacataaa aatctgacct ggataacctc ttattctcac tggtttgatg
     1561 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     1621 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     1681 attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa ctgacggatc
     1741 tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag atgcaaggcg
     1801 tggtaagaga gtttatcgac agcagccagc caccgcttag cgaggtaaat caggagaagt
     1861 ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggatt
     1921 tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagccc tccagttttc
     1981 aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg
     2041 aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc gaagcgtctg
     2101 gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc ctggattttt
     2161 tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt tttaatgcat
     2221 tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ctttctcaaa gaacatgaat
     2281 ttctggttgg tatctcgatc gatggccccc aggagttaca tgaccgttac agacgcagta
     2341 attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc
     2401 aagtagagtt taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg
     2461 tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctagaaa
     2521 ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt
     2581 tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg caatgggtta
     2641 aaaacgatgt gggtgaaatt ttcattcgtc agtttgaaag ctttgtcagc cgttttttgg
     2701 ggaatgggca tacaagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa
     2761 gtaatggaga catttacgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca
     2821 ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga
     2881 tttcagcgaa atgtcagcaa tgtgcatata aacctatctg caatggcggt tgtcctaagc
     2941 atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt tataaaatcc
     3001 ttttttcaac catggtacct tatatgaacg ccatggttga gttagctaag aacagagtac
     3061 cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa tgctctcttt
     3121 taactccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat aaattcctgt
     3181 cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca
     3241 gggcattagc aatacccttg attatcaata gcataaccat aaccataacc actgaaaatt
     3301 aaaaatttct atatatttca tattgttaaa tgatacttaa ataaatttca tcagtaaatc
     3361 attgccatag ataatcattg gatggcacaa tcaattttcg ctaatgataa ccattcccat
     3421 tacgttttat atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa
     3481 gaataataaa acttcagtgt tgttaatcat tattatcctc gcaatgatcc ttggcgtggt
     3541 taaaattcag gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa
     3601 agaaaccgat aagctttggc agcttgttct ttggttccct gccctgctgg ggatctttgt
     3661 attaatatcc gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg
     3721 gttaacagat tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat
     3781 ctacggcgag cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt
     3841 actgatcagc aaaacgctca gtctttcatt tggcttcatc cagtcactta gcatgctgat
     3901 cacctttacc gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac
     3961 agaatggaat atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac
     4021 tttatttacc cataaggtgg gtaaacgtat tcgtccgctt aatgtggaaa aacaacgtag
     4081 cgaagcgaca tttcgaacta atcttgtgca gcataataag cagacagaac tgattgcgct
     4141 aagcaatgct gagtctctac aacgccagga gttgcgcgac aattttcata ccattaaaga
     4201 gaactggcat cgtttaatga atcgtcagcg gtggcttgat tactggcaaa atatttattc
     4261 gcgctcgctt agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat
     4321 caatctgggc ggactgatga aatcgcgcca ggcatttatg ctagtatcga acaatttaag
     4381 ctggtttatt tataaatatg acgaacttgc tgaactggct gcggttatcg atcgcttgta
     4441 tgagttccat caactcactg aacagcgccc tacgaataag cctaaaaatt gccaacatgc
     4501 ggtacaagtg gctaatgcga gtattcgtac gcctgataat aagatcatat tagagaactt
     4561 gaactttcat gtttcgccag gcaaatggct attactgaaa ggctactctg gtgcgggaaa
     4621 aaccacactg cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atatttcttc
     4681 tcctgctgac agttggtatg tgtcacaaac accgttaatc aaaagcggct tactgaaaga
     4741 gattatttgt aaagcacttc ccctgcccgt agacgataaa tcgttgagcg aagtactgca
     4801 tcaggttggt ctggggaaat tggctgcgcg tattcatgac cacgatcgct ggggagatat
     4861 tctttccagc ggcgaaaaac aacgtatcgc cctggcacga ttaattttac gacgtccgaa
     4921 atggatattt cttgacgaaa ctacctctca tcttgaggaa caagaggcta tacgcttact
     4981 gcgtttagtg cgtgaaaaac tacccacaag cggcgtcatt atggttacac atcaacccgg
     5041 tgtctggaac ctggctgatg atatttgtga cattagcacg gttttataac aacaataaaa
     5101 gccctgctgt acggagatat aaataaatga agcgagttct tattcctggc gtcattttat
     5161 gtggcgctga tgtggcgcag gccgtcgatg acaaaaacat gtacatgcat ttttttgaag
     5221 agatgacggt ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg
     5281 aaagcatcga gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc
     5341 ctgcggtacg catggattca acgcaaagta cctcgttgaa ccagggagat attcgccctg
     5401 agaaaatctc tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta
     5461 gtgcaactaa taacctgaac ccagcgaatg agtccgatgc cagtagtgca accaatatta
     5521 gcgggatgtc acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg
     5581 acagttttgt gccggttgaa tttggtcgct tcaatggcgg ggtaattgat gcaaagatca
     5641 aacgcttcaa cgctgatgat agcaaggtga aattgggtta tcgcactacg cgttcggact
     5701 ggttaacatc gcatatcgat gagaataaca agagcgcatt taatcaaggc tcttcaggaa
     5761 gtacttatta ctccccagat tttaaaaaga acttttatac cttgtcgttt aatcaggaac
     5821 tcgctgataa cttcggcgtt accgccggtt tatcgcgccg ccagtctgat atcacccgcg
     5881 cggattatgt ttcgaatgac ggcattgtca ccggtcgggc acagtataaa aacgttatcg
     5941 atactgcatt gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct
     6001 taaaatatac cggctccagc cgtgattata ataccagcac cttcccgcag tctgatcgcg
     6061 aaatgggtaa taaatcctat ggtctggcat gggatatgga tacgcaactc gcatgggcca
     6121 aactacgtac caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca
     6181 tctggtacac cgaactttca tgtacatatg gtgatattac agggcgttgt acctgtggcg
     6241 gattaggaca catttcccag gctgtagata attacacctt caaaacacgc ctggactggc
     6301 aaaaattcgc cgtgggtgat gtttcgcatc aaccctactt cggcgcggaa tacatctatt
     6361 ccgatgcatg gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgcaggaa
     6421 agaaaactaa ccataccatt taccataaag gtaaaggcag cctgggaatt gacaactaca
     6481 cgctgtatat ggcggagcac attagctggc ggaatgtgtc gttaatgccc ggtgtgcgtt
     6541 atgactatga caactatctg tcaaaccaca atatctcccc gcgctttatg acggaatggg
     6601 atatttttgc tgatcaaacc tcaatgatta ccgccggtta taaccgttac tatggcggga
     6661 atattcttga tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggta
     6721 ataaaactct gacgcgttat caggatttga aaacgcctta taacgatgaa ctggcaatgg
     6781 gattgcagca gaaaatcggt aagaacgtta ttgcgcgtgc aaactatgtt taccgtgaag
     6841 cgcatgatca aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg
     6901 aatataacaa cgatggcaaa accaaaacgc attcgttcag cctcagtttt gaactggccg
     6961 aacccctgca tatccgccaa gtagatatta acccacaaat tgtctttagc tatatcaaga
     7021 gcaagggcaa cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccagg
     7081 tggtttataa cggtaatctg gtctcttacg atagtgttcc agtggcagat tttaataacc
     7141 cattaaagat ctccttaaac atggatttca cgcatcaacc gagcgggtta gtgtgggcga
     7201 atacgctggc ctggcaagaa gcgcgtaaag ctcgcattat ccttggtaag acaaatgcgc
     7261 aatacatcag cgaatattca gattacaagc agtatgttga cgaaaaactg gatagcagcc
     7321 tgacctggga cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga
     7381 tcagtgccga tattctcaat gtactggata gcaaaaccgc tgttgataca acgaacaccg
     7441 gtgtggcgac ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat
     7501 agttcggatt ttacttaacc gggtaaattc gcccggtttt cgcatggaga taattatgag
     7561 aaacctctgt ttcttactga cgttagtggc aactctgttg ctccccgggc ggctgattgc
     7621 cgccgcctta ccgcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata
     7681 tatgatttat ccgcatgctc aaccaaagga tcaggtaaat ttatggctgc aaattcatac
     7741 cggttcattg caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat
     7801 gtttaacggc acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg
     7861 cctgcgtttc ggtcgcgatg ttaatgccta taccagctat gacgaaacgc tgtatcaggt
     7921 gagtctgccg actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg
     7981 gagtaatgcc gcaacctttg aaatactcga agtagacgct gaacgcggcg taattactga
     8041 ggaatggcgt gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct
     8101 gctggcaaat acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac
     8161 ggtcacaccg gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac
     8221 ctttatcgtg gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt
     8281 aagtaagctt ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa
     8341 ccacctgcgc tttaatatca ttaatgataa agaaaaccga gtgaacggca tcgcactcta
     8401 ttatcgcctg ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg
     8461 gagcatgtta gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt
     8521 gaagactatt tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct
     8581 gtttttccgt gtaaatgcac gagacgataa tatgcaggat gctgcaaatg cattaatggc
     8641 agagttggca accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc
     8701 tacccgcctc acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat
     8761 gctgaccagt cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga
     8821 gacatatcaa ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa
     8881 atggcagcag ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct
     8941 tgccgccaaa aaagcattgt ctcttgcggc tatcctggcg ctggaaaaag agtacgccaa
     9001 caaaaagctg gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga
     9061 cccacaggcg gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact
     9121 ttccaatggt gccagggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat
     9181 tactgccgta tctaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc
     9241 gctggcaaat aaagcagtaa gcggaagcgg cgttggtgag ctctcctctt ccagcctgaa
     9301 acgctggagt gcggaaaatt cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt
     9361 gctctccgtt agcgcgcgga ctaataaccc tgaacctggt tttcagttga ttaaccagcg
     9421 aatcacccac agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca
     9481 ggcgttgaaa acgctcgacc agcgtccagc ggagaaattc gcccagcaga tgtatgagac
     9541 gcgctatgct gatgaccgca cgaaattact gcaagaaaat cagattgtac agtttactgc
     9601 cgcagatgcg ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt
     9661 cattgtcggt aatgtctcag aagacaaact cgtggcgtta attacgcgtt acttaggatc
     9721 aatcaaacac tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa
     9781 cgcatcggtt actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg
     9841 ttatgattcc cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt
     9901 cgcactggca aaagatctac gcgttaatat tcgtgaacag gcatctggag catacagcgt
     9961 ttcttctcgc ctctcggttg atcctcaggc caaagatatc agtcatttgc tggcttttac
    10021 ttgtcaacca gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg
    10081 tctggctaaa gggatcagtg agcaagaact gaatgaatac cagcaaaacg ttcagcgcag
    10141 cctcgatatc caacagcgta gcgttcaaca attagcgaac actattgtaa atagtcttat
    10201 tcaatatgac gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt
    10261 agagaatgtt aacactgccg ttaaacaata tctttctcat ccggtaaata cttatactgg
    10321 agtattattg ccaaaataat aacagccccg tcaacacatc gttggcgggg attttagcaa
    10381 ta
//
LOCUS       DAZOZY010000143        10358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZOZY010000143 DAZOZY010000000
VERSION     DAZOZY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.143"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            207..1601
                     /gene="galP"
                     /locus_tag="ACHURQ_003073"
     CDS             207..1601
                     /gene="galP"
                     /locus_tag="ACHURQ_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR0824792.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1678..2175
                     /locus_tag="ACHURQ_003074"
     CDS             1678..2175
                     /locus_tag="ACHURQ_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR0824793.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2270..2977
                     /gene="endA"
                     /locus_tag="ACHURQ_003075"
     CDS             2270..2977
                     /gene="endA"
                     /locus_tag="ACHURQ_003075"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR0824794.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            3057..3788
                     /gene="rsmE"
                     /locus_tag="ACHURQ_003076"
     CDS             3057..3788
                     /gene="rsmE"
                     /locus_tag="ACHURQ_003076"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR0824795.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3801..4751
                     /gene="gshB"
                     /locus_tag="ACHURQ_003077"
     CDS             3801..4751
                     /gene="gshB"
                     /locus_tag="ACHURQ_003077"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR0824796.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4860..5423
                     /locus_tag="ACHURQ_003078"
     CDS             4860..5423
                     /locus_tag="ACHURQ_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR0824797.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5423..5839
                     /gene="ruvX"
                     /locus_tag="ACHURQ_003079"
     CDS             5423..5839
                     /gene="ruvX"
                     /locus_tag="ACHURQ_003079"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR0824798.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6014..6994)
                     /locus_tag="ACHURQ_003080"
     CDS             complement(6014..6994)
                     /locus_tag="ACHURQ_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR0824799.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            7012..7716
                     /gene="yggS"
                     /locus_tag="ACHURQ_003081"
     CDS             7012..7716
                     /gene="yggS"
                     /locus_tag="ACHURQ_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR0824800.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7734..8300
                     /gene="yggT"
                     /locus_tag="ACHURQ_003082"
     CDS             7734..8300
                     /gene="yggT"
                     /locus_tag="ACHURQ_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR0824801.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8297..8587
                     /gene="yggU"
                     /locus_tag="ACHURQ_003083"
     CDS             8297..8587
                     /gene="yggU"
                     /locus_tag="ACHURQ_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR0824802.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8595..9188
                     /locus_tag="ACHURQ_003084"
     CDS             8595..9188
                     /locus_tag="ACHURQ_003084"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR0824803.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9181..10317
                     /gene="hemW"
                     /locus_tag="ACHURQ_003085"
     CDS             9181..10317
                     /gene="hemW"
                     /locus_tag="ACHURQ_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR0824804.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDNALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         2428 a         2724 c         2741 g         2465 t
ORIGIN      
        1 gttacatttc ttttcagtaa agtcttaatt gcagataaca gcgtttaatc tatgatgata
       61 taactcaatt attttcatgc acttaaacca taactaagat aaatgttagt gtaagcgatt
      121 acactgatgt gatttgcttc acatcttttt acgtcgtact cacctatctt aattcacaat
      181 aaaaaataac catattggag ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca
      241 aggcaatgac gtttttcgtc tgcttccttg ccgctctggc gggattactc tttggcctgg
      301 atatcggtgt aattgctggc gcactgccgt ttattgcaga tgaattccag attacttcgc
      361 acacgcaaga atgggtcgta agctccatga tgttcggtgc ggcagtcggt gcggtgggca
      421 gcggctggct ctcctttaaa ctcgggcgca aaaagagcct gatgatcggc gcaattttgt
      481 ttgttgccgg ttcgctgttc tctgcggctg cgccaaacgt tgaagtactg attctttccc
      541 gcgttctact ggggctggcg gtgggtgtgg cctcttatac cgcaccgctg tacctctctg
      601 aaattgcgcc ggaaaaaatt cgtggcagta tgatctcgat gtatcagttg atgatcacta
      661 tcgggatcct cggtgcttat ctttctgata ccgccttcag ctacaccggt gcatggcgct
      721 ggatgctggg tgtgattatc atcccggcaa ttttgctgct gattggtgtc ttcttcctgc
      781 cagacagccc acgttggttt gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc
      841 tacgcctgcg tgacaccagc gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt
      901 tgcaggttaa acagagtggc tgggcgctgt ttaaagagaa cagcaacttc cgccgcgcgg
      961 tgttccttgg cgtactgttg caggtaatgc agcaattcac cgggatgaac gtcatcatgt
     1021 attacgcgcc gaaaatcttc gaactggcgg gttataccaa caccacagag caaatgtggg
     1081 ggacagtgat tgtcggcctg accaacgtac ttgccacctt tatcgcaatc ggccttgttg
     1141 accgctgggg acgaaaacca acgctaacgc tgggcttcct ggtgatggct gctggcatgg
     1201 gcgtactcgg tacaatgatg catatcggta tccactctcc gtcggcgcag tatttcgcca
     1261 tcgccatgct gctgatgttt attgtcggtt ttgccatgag tgccggtccg ctgatttggg
     1321 tactgtgctc cgaaattcag ccgctgaaag gccgcgattt tggcatcacc tgctccactg
     1381 ccaccaactg gattgccaac atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc
     1441 tgggtaacgc caacaccttc tgggtgtatg cggctctgaa cgtactgttt atcctgctga
     1501 cattgtggct ggtaccggaa accaaacacg tttcgctgga acatattgaa cgtaacctga
     1561 tgaaaggtcg taaactgcgc gaaatcggcg ctcacgatta atctcctcag gcttcctccc
     1621 gtcgcggagg aagccgcctc ttgcagtcat cttttcttcg ctctatcctc tgccgctatg
     1681 aaaacatccc gtctccctat cgccatccaa caggccgtta tgcgcggcct gcgggaaaaa
     1741 ctcgcccagg ccaacctgaa gctagggcgt aactacccgg agccaaaact ctcttacacc
     1801 cagcgcggaa cctccgccgg aacagcctgg ctggaaagct atgaaatccg cctcaatccc
     1861 gttttgctgt tggaaaacag tgaagctttt attgaagaag tggtaccgca cgaactggca
     1921 catttgctgg tatggaaaca tttcggccgc gtagcgccac atggcaaaga gtggaagtgg
     1981 atgatggaaa gcgtgctggg tgttcccgcc cgtcgtacgc atcagttcga actgcaatcc
     2041 gtgcgtcgca acaccttccc ctaccgctgc aagtgccagg agcatcagct taccgtacgc
     2101 cgccataatc gcgtagttcg tggcgaggcc gtctatcgct gtgttcactg cggtgaacag
     2161 ctggttgcga aataaccatc tgaactatca ggaactttcc tgatctggct gattgcatac
     2221 caaaacaact ttcgctacgt tgctggctcg ttttaacacg gagtaagtga tgtaccgtta
     2281 tttgtctatt gctgcggtgg tactgagcgc agcattttcc ggcccggcgt tggccgaagg
     2341 tatcaatagt ttttctcagg cgaaagccgc ggcggtaaaa gtccacgctg acgcgcccgg
     2401 tacgttttat tgcggatgta aaattaactg gcagggcaaa aaaggcgttg ttgatctgca
     2461 atcgtgcggc tatcaggtgc gcaaaaatga aaaccgagcc agccgcgtag agtgggaaca
     2521 cgtcgttccc gcctggcagt tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa
     2581 aaactgcgct aaagatccgg tctatcgcaa gatggaaagc gatatgcata acctgcagcc
     2641 gtcagtcggt gaggtgaatg gcgatcgcgg caactttatg tacagccagt ggaatggcgg
     2701 cgaaggccag tacggtcaat gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc
     2761 accagcgcgt gcacgcggtg ccattgcgcg cacctacttc tatatgcgcg accaatacaa
     2821 cctgacactc tctcgccagc aaacgcagct gttcaacgca tggaacaaga tgtatccggt
     2881 taccgactgg gagtgcgagc gcgatgaacg catcgcgaag gtgcaaggca atcataaccc
     2941 gtatgtgcaa cgcgcttgcc aggcgcgaaa gagctaacct acactagcgg gattcttttt
     3001 gttaacccct accccacgcg tacaaccgcg tggggagacg acgcggattt ttaactatgc
     3061 gtatcccccg catttatcat cctgaaccac tgaccagcca ttctcacatc gcgctttgcg
     3121 aagatgccgc caaccatatc gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat
     3181 tgtttgacgg tagcaaccag gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg
     3241 tggaagtgaa ggtgctggaa ggcaagatcg acgatcgcga atctccgctg catattcacc
     3301 tcggtcaggt gatgtcgcgt ggtgaaaaaa tggaatttac gatccagaaa tcgatcgaac
     3361 tcggtgtaag cctcattacg ccactttttt ctgagcgctg cggcgttaaa ctggatagtg
     3421 aacgtctgaa caagaagctc cagcagtggc agaagattgc aattgctgcc tgtgagcagt
     3481 gtggtcgtaa ccgggtgccg gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag
     3541 agcaggatga aggactgaaa ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt
     3601 tgccgttacc ggttgaacgc gtccgcctgc tgattggccc ggaaggcggt ttatcggcag
     3661 atgaaattgc catgactgcc cgctatcaat ttactgatat cctgttggga cctcgcgttt
     3721 tgcgtacaga gacaactgcg ctcaccgcca ttaccgcgct acaagtacga tttggcgatt
     3781 tgggctaacg gagaagaata atgatcaagc tcggcatcgt gatggacccc atcgcaaaca
     3841 tcaacatcaa gaaagattcc agtttcgcta tgttgctgga agcacagcgt cgtggttacg
     3901 aacttcacta tatggagatg ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata
     3961 cccgcacgct gaacgtgaag cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg
     4021 atctgccgct ggccgatctc gatgtgatcc tgatgcgtaa agacccgccg tttgataccg
     4081 agtttatcta cgcgacctat attctggaac gtgccgaaga gaaagggacg ctgatcgtta
     4141 acaagccgca gagcctgcgc gactgtaacg agaaactgtt taccgcctgg ttctctgact
     4201 taacgccaga aacgctggtt acgcgcaata aagcgcagct aaaagcgttc tgggagaaac
     4261 acagcgacat cattcttaag ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga
     4321 aagaaggcga tccaaacctc ggcgtgattg ccgaaaccct gactgagcat ggcactcgct
     4381 actgcatggc acaaaattac ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg
     4441 tggatggcga gccggtaccg tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg
     4501 gcaatctggc tgccggtggt cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa
     4561 tcgcccgtca gatcgggccg acgctgaaag aaaaagggct gatttttgtt ggtctggata
     4621 tcatcggcga ccgtctgact gaaattaacg tcaccagccc aacctgtatt cgtgagattg
     4681 aagcagagtt tccggtgtcg atcaccggaa tgttaatgga tgccatcgaa gcacgtttac
     4741 agcagcagta acccacctta gcgagaagga tctcgttgag actctgagtg acagcgccct
     4801 tctttccacg catactgggc gctgttgctt ttttgaacca ggaaacagaa cctctgacaa
     4861 tgaatttaca gcatcacttt cttattgcca tgcctgctct ccaggatccg attttccgtc
     4921 gttccgtggt ctacatttgc gaacataata ccaatggtgc aatggggatc atcgtcaaca
     4981 agccgctgga aaatctcaaa attgaaggga ttctggaaaa gctgaagatc acgccggagc
     5041 cgcgtgatga atcaatccgt ctggataaac cggttatgct cggcggtccg ctggctgaag
     5101 atcgcgggtt tattttgcat actccgccct ccaattttgc ttccagcatt cgcatttcag
     5161 acaacacagt gatgaccact tcccgcgatg tgctggaaac gctcggcacc gataaacaac
     5221 cgtctgacgt attggtggct ctaggttatg cctcctggga aaaaggtcaa ctggaacaag
     5281 aaattctcga taacgcgtgg ctaacggccc cggcggatct gaatattctg ttcaaaacgc
     5341 cgattgccga ccgctggcgc gaggcggcaa aactgattgg tgtggatatt ctcaccatgc
     5401 ctggtgtggc agggcacgcc tgatgagtgg aaccttactc gccttcgact tcggcaccaa
     5461 aagcattggc gtagcggtcg gccaacgcat taccggcact gctcgccctt tgcctgcaat
     5521 taaagcacag gacggtacgc cggactggaa cctcatcgaa cgtttactga aagagtggca
     5581 gccggacgaa atcatcgtcg gtttgccgct gaatatggac ggcaccgagc aaccattgac
     5641 tgccagagcg cgtaaatttg ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct
     5701 ccatgacgaa cgtcttagca ccgtggaagc ccgttccggt ctgtttgaac agggcggcta
     5761 tcgggcgctc aacaaaggca aagtcgactc tgcctctgcg gttattattc tcgaaagcta
     5821 tttcgagcag ggatattaag gcgatttaaa agcctggcgg atagtcgtta ccgccatctt
     5881 gccggatgcg gcgtgaacgc ctatccggcc tacatgtggc agcgattgta ggtctgataa
     5941 gacgcgcaaa cgtctcatca gacgtttatt gcctttgtag gcccgataag cttgcacatc
     6001 gggcatggca acgtcacaaa cgcccttccc ccactcgctg ctgaaaactc tgctgaaacg
     6061 ttatcatccc cacctgctgc ccggtttgaa taacatgcgg taactggtgg gttttccctt
     6121 cgcgaatcaa attccccacc gcgggagtgt taatcaataa ttcaaacagc gccacgcgtc
     6181 cttcctgttt atccacttcc agcttttgtg acaacacggc ccgtaaacta cctgccagtt
     6241 gattacgtac gggatctttt tcctgcgccg gaaatgaatc caccagtcgc tcaactgcct
     6301 gcgcggcacc acgcgtatgt aatgttgcca gcaccaaatg cccggtttct gccgccgtca
     6361 gtgccagacg gattgtctcg ctgtcacgca gctctccgag caaaatcaca tcaggatctt
     6421 cccgcaatgc ggcccgcaat cccgatgcga acgtcataca gtgcaaacca atttcccgct
     6481 tctggatcaa acatcgctgg ctggcataga tatattcaac aggatcttcc agcgtcagaa
     6541 tatgcgcatc ggcatgttga ttgagatagc caaccatcgc cgccagcgtg gtagatttgc
     6601 tgctccccgt cgcccccgtc accagaatca ggccattctc actcttgagt aattccggca
     6661 ataccgttgg tgcgccaagc tgttcgagct gcgggcagtg cgaaggtaac agccgtaacg
     6721 ccagcgaaat gccatgccgt tgcgcaaatg cgctgccacg caatcgctgg ttttccgcca
     6781 gcgacacggc aaaatccagc tggccatttt ccagcaatat tgcccgctga tcgtcatcca
     6841 gccactcccg cagtagctct tcgacgtccg gcgcctcaaa cggcgcagct tccattagcc
     6901 cgcgaatgcg ccatcgtgcg ggccaggcgc tgcacaggtg tagatccgag acgttatgct
     6961 ttacactaag ggccacaatt tcttccatat tcatactaag atcctcggaa aatgaacgat
     7021 attgcgcata acctggcaca ggtccgggac aaaatctcag cggctgcaac gcgttgcggc
     7081 cgttctccag aagaaattac gctgcttgca gtcagtaaaa caaaacctgc gagcgccatc
     7141 gcagaagcca ttgatgccgg gcagcgtcaa tttggtgaaa actacgttca ggaaggggta
     7201 gataaaattc gccactttca ggaactgggc gtaacaggat tagaatggca ttttattggc
     7261 ccgttgcagt ctaataaaag ccgcctggtg gcagagcatt tcgactggtg tcataccatc
     7321 gaccgtttgc gcattgctac ccgcctcaac gatcagcgcc cggcagaact tccccctctt
     7381 aacgtactga ttcaaattaa tattagtgat gaaaacagta agtccgggat tcaactggca
     7441 gaactggacg agctggcagc ggcggtcgct gaactaccgc gtttacgtct gcgcgggctg
     7501 atggcaatcc ctgcgcctga gtcagaatat gtaaggcagt ttgaagttgc acgccaaatg
     7561 gctgtagcat ttgccggact gaaaacgcgc tacccgcata tcgacacgct ctctctggga
     7621 atgtcggacg atatggaagc cgccattgcg gcaggtagca cgatggttcg tatcggcact
     7681 gcaatttttg gtgcgcgtga ttactctaaa aaataaggaa ttaaaggaac gccatgaata
     7741 cgttgacttt cctgctttca acggtcattg agctgtatac catggtgctg ttattacgca
     7801 tctggatgca gtgggctcat tgtgattttt acaacccctt ctcacagttt gtagtgaagg
     7861 taacgcagcc aattatcggg ccactgcgcc gcgttattcc ggcaatgggc cccattgaca
     7921 gcgcctcgct gctggttgcc tatattctca gttttatcaa agccatcgtg ctgtttaaag
     7981 tggtgacctt cctgccaatc atctggattg ctggtttact gattctgctg aaaaccatcg
     8041 gcctgctgat tttctgggtc ctgctggtga tggcgattat gagctgggta agccagggtc
     8101 gtagtccgat tgaatacgtg ctgattcagc tggccgatcc gctgctgcgc ccgattcgcc
     8161 gcctgctacc ggcaatgggt gggattgatt tctcgccgat gatcctcgtt ctgctgctgt
     8221 atgtcatcaa tatgggtgtc gcagaagtat tacaggcaac cggaaacatg ctgctgccgg
     8281 ggctgtggat ggcgttatga gtgccgtaac agttaatgat gacggtctgg ttttacggct
     8341 ctatattcag ccgaaagcca gccgtgattc tattgtcggt ttacatggcg acgaagttaa
     8401 agtcgccatt accgcgccgc cggttgacgg ccagggcaac agtcatctgg tgaagtttct
     8461 cggtaagcaa ttccgggttg ccaaaagcca ggtggtgatt gaaaaaggcg aacttggccg
     8521 ccacaaacaa attaaaatca ttaatccgca acaaatcccg ccagaaatcg cggcgttaat
     8581 taattaggta tcctatgcaa aaagttgtcc tcgcaaccgg caatgccggt aaagtgcgtg
     8641 agctggcgtc gctgcttagc gacttcggtc ttgatatcgt ggcccaaaca gacctcggcg
     8701 tagattccgc tgaagaaacc ggcctgacct ttatcgaaaa cgcgattctg aaagcgcgct
     8761 atgcggcaaa agtgaccggt ttaccggcaa ttgccgacga ctctggtctg gcggtagatg
     8821 cgctgggtgg cgcgccgggt atttactccg cgcgttattc cggtgaagac gcgaccgatc
     8881 aaaagaatct gcaaaaactg ttggaaacac tgaaagacgt accggacgac caacgtcagg
     8941 cgcgtttcca ctgcgtgctg gtatatctgc gtcacgcgga agatccgact ccgctggtat
     9001 gccacggcag ctggccgggc gtgattactc gcgaaccagc gggcactggt ggctttggtt
     9061 atgatccaat cttcttcgta ccttccgaag ggaaaaccgc cgccgaactg acccgcgaag
     9121 aaaagagcgc catttcccac cgtggtcagg cgttgaaact gctgctggac gctttacgta
     9181 atggttaaat taccgccgct gagtctctac attcacatcc cgtggtgcgt gcagaaatgc
     9241 ccgtactgcg atttcaactc tcacgcgttg aaaggagaag tgccgcacga cgattatgtt
     9301 cagcatctgc tttgcgatct ggacaatgat gtggcttacg ctcagggccg tgaagtaaag
     9361 acaatcttta ttggcggtgg tacgccgagc ctgctttccg gcccggcgat gcaaacgctg
     9421 ctggacggcg tgcgtgcgcg tttgccgctg gcagcggatg cagaaattac tatggaagcg
     9481 aaccctggaa cggtagaagc cgatcgcttt gtcgattatc agcgtgctgg tgtgaaccgc
     9541 atctctattg gtgtgcagag ttttagcgaa gaaaagctga aacgacttgg gcgtattcat
     9601 ggcccgcaag aagcgaaacg cgcggcaaag ctggcgagcg ggctggggct gcgtagcttt
     9661 aacctcgatt tgatgcatgg actaccggat caatcactgg aagaggcgct tggcgatcta
     9721 cgccaggcca ttgaactgaa tccgccgcat ctttcctggt atcaactgac catcgaaccc
     9781 aatacgctgt ttggttcgcg accaccggtg ctgccggacg ataacgcgct gtgggatatt
     9841 ttcgaacagg ggcatcagtt attaaccgca gcgggttatc agcaatatga aacttccgct
     9901 tacgccaaac ccggttatca gtgccagcac aatctcaact actggcgatt tggtgactac
     9961 atcggtattg gctgcggcgc gcacggcaaa gtcaccttcc cggatgggcg cattctgcgt
    10021 accactaaaa cgcgtcatcc tcgtggtttt atgcaaggaa ggtatctgga aagccagcgt
    10081 gatgtcgaag ccgcagataa gccgtttgag ttctttatga atcgcttccg tctgctggag
    10141 gccgcgccgc gcgtggagtt tattgcgtat accgggcttt gcgaagatgt gattcgccca
    10201 cagttagacg aggcgattgc ccagggttat ctcaccgaat gtgcggatta ctggcagata
    10261 accgagcacg gtaaattatt cttaaactca cttcttgaat tattcctcgc tgagtaatca
    10321 ttaatgcgcc tgcgggcgca ttaatcactt cttttccc
//
LOCUS       DAZOZY010000144        10087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZOZY010000144 DAZOZY010000000
VERSION     DAZOZY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.144"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..309
                     /locus_tag="ACHURQ_003086"
     CDS             <1..309
                     /locus_tag="ACHURQ_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR0824805.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="ACHURQ_003087"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="ACHURQ_003087"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR0824806.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHURQ_003088"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="ACHURQ_003088"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR0824807.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHURQ_003089"
     CDS             2501..3394
                     /gene="ilvY"
                     /locus_tag="ACHURQ_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR0824808.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHURQ_003090"
     CDS             complement(3446..4990)
                     /gene="ilvA"
                     /locus_tag="ACHURQ_003090"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR0824809.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHURQ_003091"
     CDS             complement(4993..6843)
                     /gene="ilvD"
                     /locus_tag="ACHURQ_003091"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR0824810.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHURQ_003092"
     CDS             complement(6908..7837)
                     /gene="ilvE"
                     /locus_tag="ACHURQ_003092"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR0824811.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHURQ_003093"
     CDS             complement(7857..8120)
                     /gene="ilvM"
                     /locus_tag="ACHURQ_003093"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR0824812.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHURQ_003094"
     CDS             complement(8117..9763)
                     /gene="ilvG"
                     /locus_tag="ACHURQ_003094"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR0824813.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHURQ_003095"
     CDS             complement(9766..9816)
                     /gene="ilvX"
                     /locus_tag="ACHURQ_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR0824814.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHURQ_003096"
     CDS             complement(9903..10001)
                     /gene="ilvL"
                     /locus_tag="ACHURQ_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR0824815.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
BASE COUNT         2225 a         2825 c         2666 g         2371 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 gacctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctagt acaacaagtc
      181 agtcgaaacc gattaaacag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catatcgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactaac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg tttgataaag ttgtgttctc ttgtccggta
      721 ctggagccaa ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca gcggcacaca agcgtaagag
     1081 aacagatagt taccgtactc ggcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agttcgtgca gtgattcata gtaagcagat
     1201 tcttcgatga tgccggaatc aaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agagagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgta cttcggtgcc agggcatttc ggcgcaacca tgactacggt aatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacatcgag ggctgcgcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac tcaccactgg
     2881 tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg cgattgccgg
     2941 gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcc cccgcgctgc cctgcccggt gcgtaatcag gtatctgtcg aaaagcccga
     3061 ctggtcaacg gtgccgttta ttatggccga tcaggggccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggtgtggcg ctgttgccgg aagtggtgct
     3241 ggaaaacagc ccggagcctg tacgcaaccg ggtaatgatt ttagatcgca gcgatgagaa
     3301 aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta ggcctgataa
     3421 gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac gccggattat
     3481 tggtttcgtc gtggcaatcg tagcccagct catttagccg ggtttcgaaa tccggttcat
     3541 ggtcgccgag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt
     3601 ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg
     3661 gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc
     3721 cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt
     3781 agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc
     3841 gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga
     3901 ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg
     3961 tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca
     4021 ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc caggcgttcg ccgcgaatgt
     4081 tgtgctgagc gatatatttt ttcattcccg ccagcgccaa cgcgccagag ggttccgcca
     4141 ctgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca ctatcgacgg
     4201 tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcgccg atgcgtttta
     4261 ccgcgacacc ttcggcgaac agtcccacgc gcggcagatc aaccggatga cccgcatcca
     4321 gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg atttgtggca
     4381 tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg actggcacaa
     4441 atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg
     4501 caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt
     4561 cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga
     4621 atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct
     4681 tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag
     4741 tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc
     4801 gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa
     4861 gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg
     4921 gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg
     4981 agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct
     5041 ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac ggtttttcgg
     5101 cgtccaggct ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc
     5161 gcttacctgt aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc
     5221 aatcagacca atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc
     5281 agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat
     5341 tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc
     5401 ttcatagcgg atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac
     5461 cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc
     5521 gacgcccgct gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag
     5581 gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc
     5641 atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg
     5701 gcctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc
     5761 cagagtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc
     5821 gcgatccagt tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg
     5881 gtatttttgg gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc
     5941 gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac
     6001 cgtgttggta gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt
     6061 gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa
     6121 ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag
     6181 cagcgagccg ttgcctggct gcgacaaacc cagcgcttcg gtcaggcagt tcattgagtt
     6241 agcggtaaac atcccggagc aggaaccgca ggttgggcag gcggaacgtt caacctgatc
     6301 gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag
     6361 cttgatgatc tgctcggaca gtttggtttt cccggcttcc atcgggccgc cggaaacaaa
     6421 gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca
     6481 gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc
     6541 agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca tagcaatccc
     6601 atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc
     6661 ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga
     6721 gttcacaacc gcgataatcg gcttgccaaa atcggcatcg gtcattccgg tggcgcgcca
     6781 cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg
     6841 catactttat ttactcccag tgtctgtctc gtaaatggga cggtaagtgc cgtcccattt
     6901 tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga
     6961 agaggccgaa gaaggcttgc tgaatgcatt tggtaaccgg gccgcaacgg ccttcaccca
     7021 cctgaatacc gtctacactg cgcactggcg tgatttctgc cgccgtaccg gacataaaca
     7081 cttcatccgc caggtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca
     7141 gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg gatgaggtaa
     7201 acggtggggt gaacagcacg ccgtctttca cttcaaacag gttttcacct gcgccttcag
     7261 agatgtaacc gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc
     7321 tacccaccag cagggaagag aggtagttac caccggcttt tgccgccgtc gggatggtgt
     7381 ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg
     7441 cgcccagata cgctccccac gggaaagcag cgataatcac gtcggttgag tatcccgctg
     7501 gcgggtttac tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg
     7561 tgagattatt tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgcg
     7621 aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc
     7681 ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa accgaagtgc
     7741 catagtgcag cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc
     7801 cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag
     7861 gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc
     7921 agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg
     7981 atatttatat tttgtgcatc gctggcggcg gccatattca ttgagcagac gtggaaacca
     8041 cgatgacgca ccacgcgtaa aacacgttct aaggtttctg gattgaagcg agccgataca
     8101 ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg gcgccaggcg
     8161 gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat
     8221 cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc
     8281 cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta agggtggttt
     8341 cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt
     8401 tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca
     8461 cattcatcat gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga
     8521 cttgtgcgcc aaccgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga
     8581 tgaaattttc cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca
     8641 catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg
     8701 gcgcgtagat agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg
     8761 cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat
     8821 ttaaatcacc ctgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga
     8881 tatccatatg gataacactg gcgtgtggtg cgaaagtatt cagtttgccg gtcacccggt
     8941 catcaaaacg tgcgcccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg
     9001 ctttgatgcc gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc
     9061 ccagcccttt cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag
     9121 ccggaactgc ctgcgccata cccacgccac cgccaacgta cagcatcggt ttttgcgctt
     9181 ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact tcgttttcaa
     9241 cggtggtgaa ccacggttcc aggtcaccgc tggctaactg gatatctttt gggatatcga
     9301 ccagaaccgg accaggacga cctgagcagg caacgtcgaa tgcttcagcc atgatgcgcg
     9361 gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac gacaatccta
     9421 ggacatccac ttcctgaaat gcgtcagtgc cgataaacgg tgcggacact tgaccggtga
     9481 tggcaacaac agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg
     9541 cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg gcataaccga
     9601 tagccgccat tgccgcaccc tgctcatgtc ggcatagcaa gtgctccacg ccgccgtcat
     9661 acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac
     9721 cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct
     9781 gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt
     9841 aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt aaggcttgat
     9901 ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat caccaccacg
     9961 ctaatcacga ctaggctaat cactcgtaga agggctgtca ttttgtcttt tcttgcatct
    10021 tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa gatatttttg
    10081 ggcctat
//
LOCUS       DAZOZY010000145        10086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZOZY010000145 DAZOZY010000000
VERSION     DAZOZY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.145"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..989)
                     /gene="cmtA"
                     /locus_tag="ACHURQ_003097"
     CDS             complement(<1..989)
                     /gene="cmtA"
                     /locus_tag="ACHURQ_003097"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR0824816.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1017..1460)
                     /gene="cmtB"
                     /locus_tag="ACHURQ_003098"
     CDS             complement(1017..1460)
                     /gene="cmtB"
                     /locus_tag="ACHURQ_003098"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR0824817.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1774..3765)
                     /gene="tkt"
                     /locus_tag="ACHURQ_003099"
     CDS             complement(1774..3765)
                     /gene="tkt"
                     /locus_tag="ACHURQ_003099"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR0824818.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4043..4801
                     /gene="loiP"
                     /locus_tag="ACHURQ_003100"
                     /gene_synonym="yggG"
     CDS             4043..4801
                     /gene="loiP"
                     /locus_tag="ACHURQ_003100"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR0824819.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4944..5864)
                     /gene="speB"
                     /locus_tag="ACHURQ_003101"
     CDS             complement(4944..5864)
                     /gene="speB"
                     /locus_tag="ACHURQ_003101"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR0824820.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(6002..7978)
                     /gene="speA"
                     /locus_tag="ACHURQ_003102"
     CDS             complement(6002..7978)
                     /gene="speA"
                     /locus_tag="ACHURQ_003102"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR0824821.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7987..8118)
                     /gene="yqgB"
                     /locus_tag="ACHURQ_003103"
     CDS             complement(7987..8118)
                     /gene="yqgB"
                     /locus_tag="ACHURQ_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR0824822.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8212..8355)
                     /gene="yqgG"
                     /locus_tag="ACHURQ_003104"
     CDS             complement(8212..8355)
                     /gene="yqgG"
                     /locus_tag="ACHURQ_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR0824823.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8466..8717)
                     /gene="yqgD"
                     /locus_tag="ACHURQ_003105"
     CDS             complement(8466..8717)
                     /gene="yqgD"
                     /locus_tag="ACHURQ_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR0824824.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            8773..9927
                     /gene="metK"
                     /locus_tag="ACHURQ_003106"
     CDS             8773..9927
                     /gene="metK"
                     /locus_tag="ACHURQ_003106"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR0824825.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2535 a         2686 c         2538 g         2327 t
ORIGIN      
        1 atagcaaagg acaccagggt agctaccgta acaccggcaa ttgtcgccag gaacgagcct
       61 ttcggcgtca gtgccaggta agcaaagata gaccccggac ttgggccagc caccagacca
      121 ccgtccagta agttaaacac ccaggtgcca gacataccgc ccgcaatcat ggcaataatg
      181 gtcagcggct tcatcagcac atacgggaaa tacagttcgt ggatcccacc gaggaagtga
      241 ataatcatcg ccccgggcgc agaacgttta ctcatccctt taccaaacaa ggtaaacgcc
      301 agcagcagcc ctaagccagg cccaggattg gacgccacca taaagaagat ggatttaccg
      361 ttaaccgaag cctgttgcat tcccagcgga taatagacgc cctgatcgat cgcattattg
      421 aggaaaagca ctttcgccgg ttcattgatg actgacagca gaggcagata acccgcatgt
      481 accagcgcct caatacactc tttgacgaga gtattggcaa ttaacaccgc cgggccgata
      541 acttcaaaac ccagcagaca aaggagcatc cccgcgatac ctaatgagaa gttattgata
      601 accatctcaa aaccggcagg tatgcgtttt tccagtgcct tatcaacata ttttatgacc
      661 agcccaccga gcggccccat aatcattgag ccaaggaaca tcgggatctc tgcgccgacg
      721 atcacaccaa tagtacctat tccgcccatg accgcgccgc gtttaccgcc gaccagatga
      781 ccaccggtag aaccaatcat cacgggcaat aaataggtaa tcatcgggcc gacaattttg
      841 gcgaaatgtt cgttaggcag ccaaccggtg ggaataaata acgcagtaat aaaaccccag
      901 gcaataaaag caccaatatt ggggatgacc attgccgtca aaaagccccc aaaagcctgg
      961 acctttgcac gagcagactt gttttccata atattatcct gtagaggaga gagtaattat
     1021 ccgcggctga ttatgtccgc taattgtttt tctgatgctg ctgttaagag ttgttcgagt
     1081 atttcttctt cgcacagtaa ttcacttaac gcctgaatag cgccaatgtg cgaatcggca
     1141 tcggcagcag aaaggccgat gagtaatttt attggttcgt cattccccgg aaaataaaca
     1201 ccttgttcaa gtaatgtcaa agacatcccg gttttaagcg ccccacattc cggtcgcgca
     1261 tgaggcattg ccacgcctgg tgcgagaata taataagggc cattgttaat ggtggaatct
     1321 ttaattgcct gaatgtaatt ctcgctgata tagtttttat ccagcaatga taccatcgag
     1381 aaatcgatag cttcctgcca atcttttgct gaatgtataa ctgagattga tgattctgga
     1441 aaatagtcac taagccgcat aaattatcct tattttattt ttagggtacc gtccggcggt
     1501 aaaaataaaa tactcgtgcg gtttaatgaa agcaattgag cagggaaacc ggtgttttga
     1561 aaacagtgaa ttattaattt cttatataac attgagttat agatataaca acaaatgtca
     1621 atacgcatat cgtgatgcat attacataat tgatatttat tgcatataaa tatttgtgat
     1681 ctacaacacg ctttatctat tgcttgtccg caaacggaca tatcaaggta ataaaaaagg
     1741 tcgccgaagc gaccttttta cccgaaatgc taattacagc agttcttttg ctttcgcaac
     1801 gacgttgtcg acggtgaagc cgaactcttc gaacagcaac tctgccggag ctgactcacc
     1861 gaaggtggtc ataccgatga tagcgccatt caggccaaca tacttgtacc agtagtcagc
     1921 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1981 agccgcatcc tgcttgtcga atgcatcggt agacggcatg gaaaccacgc gcgccttcac
     2041 gccttcggca gtcagttttt cgtaggcggc aacagccagt tcaacttccg aaccggtcgc
     2101 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     2161 gtttgccagt tgctcttcag tacgttcctg ctgcgccaga ttctgacggg agaggataag
     2221 cgcggtcgga ccgtcctgac gctcaacacc gtatttccac gcgaccgcgg attcaacctg
     2281 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctcaac
     2341 cggctggtga gtcgggccgt cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     2401 ctgacgctgt ttcatcagcg cagccatacg tacagcgtta cgtgcgtatt cgacaaacat
     2461 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     2521 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cccgcagcat cttcgttgat
     2581 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcgg agccgccgag
     2641 gaattccggc aacagcgggc cgaacgcttc aatagcattc tgagacgctt tacggctggc
     2701 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2761 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2821 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2881 atcccactgc gcatagattt cagacgggat ttcgaacggt gcgtatttcc agcccagctg
     2941 ttcgcgggtc agagcgattt ctgcatcacc cagcggcgca ccgtgggagt cgtgagtacc
     3001 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     3061 agtcactgcg cgcgcttctt ctactgcgcg tttgatggat gctgcgtcat gaccgtcgat
     3121 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtgaacca
     3181 gccttcaacg tgaccatcaa tagagatacc gttgtcatcg tagaacgcaa tcagtttacc
     3241 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     3301 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     3361 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     3421 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     3481 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     3541 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     3601 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     3661 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3721 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3781 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3841 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3901 cagaaatctc tggatcatgc tcgcatgttg cgcaatctac tcgcccgtcc gctgcgcttt
     3961 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     4021 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     4081 aacggtactg actggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     4141 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     4201 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     4261 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     4321 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     4381 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     4441 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     4501 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     4561 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     4621 tgattattct tacgatcttc tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag
     4681 ctttgaaaaa ctggcgaaac tggaagaagg ccgccaaagc tcaatgtttg acgaccatcc
     4741 ggcatccgcc gaacgcgccc agcatattcg cgatcgcatg agcgcggatg ggattaagta
     4801 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacatcaatg
     4861 gcacatttgt taccctgtgc gcattgccgg atgcgatgct gactgcatct tatccggcct
     4921 acgggtggca tcgcatctgg tgcttactcg ccctttttcg ccgcttggat atacagcatc
     4981 tccagcgcca gcgtcgccgc agccagagcg gtgatttccg actgatcgta tgccggagcc
     5041 acttccacta cgtccatccc aacaatgttg agatctttca ggccgcgtac cagtttaata
     5101 gcgcgatcgg aggtcaggcc gccaatcact ggcgtaccgg tgcctggtgc aaaagcagga
     5161 tccaggcagt cgatatcaaa agtcaggtaa accggcatat cacccacaat ctgtttcact
     5221 tgggcgataa cgtcatccac gctgcgatcg ttcacctggc aggcgtccag cacggtaaag
     5281 ccgttgtctt tatcaaactc ggtacgaata ccaatctgca cggaatgatt cgggtcgatc
     5341 agaccttctt tcggcgcggt atagaacata gtgccgtggt caaattcaca accgttcgca
     5401 taggtatcgg tgtgggcgtc aaagtgtacc agcgccattt tgccgaaatg cttcgcatga
     5461 gcacgcagca gcggcagcgt aacaaagtgg tcaccaccga aggtgagcat acgcttaccg
     5521 gcagccagca gcttctcggc gtgcgcctgc agcttttcgc tcatctcacg ggcatcgcca
     5581 aaggcatata ccagatcgcc gcagtccacg acgttcagac gctcacgcat gtcgaaattc
     5641 cacgggaagc ggttgtgttc ccaggccaga ttcgtcgaaa cctgacggat cgctgccgga
     5701 ccgtggcgac cacccgcacg accagaagtg gccatatcga acggcacgcc agtaatcacc
     5761 cagtctgcat cgctgtcata cggctggaag ttcatcggca ggcgtaaaaa accaaaggca
     5821 ttggaaacca gtgagttatc gtattgatga cctaaggtgc tcatggatgg actcctatta
     5881 caaagtcgat ataaaaaaac ccttccgcgt cgttaggccc gacgaggaag ggttggattt
     5941 gtcacaataa attgtggcgg attatcaccg ctaaattaag cggattcaag taacacagga
     6001 cttactcatc ttcaagataa gtataaccgt acaaacctgc ctcgaactct tcaaggaact
     6061 gttgttgcag ttcagcatca agatcggttt tcttcacttg atcgcggaac tgggttaaca
     6121 gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg ccttcgtcag
     6181 acagttctac ttctacgcta ccgtcaggga acacgaacac gtcaaccgct tcggtatcac
     6241 cgaacaggtt gtgcatattg ccaaggatct cctgatatgc gccgaccata aagaaaccga
     6301 gcatcggcgg attctctgga tcgtactccg gcattggcat tgtcgtggca ataccatcac
     6361 catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc agcacagcgc
     6421 gacgttccgg cacttgatcc agcccttcca gcggcagaac cgggaacaac tggtcgatcc
     6481 cccatgcgtc cggcattgac tggaacagcg agaaattgac gtacattttg tccgccatac
     6541 gttcctgcag ttcgtcgatt atcggacgat gcgcacggtt ttgcggatcc agttgcttct
     6601 gcacttcatg gcacatgctc agatagagtt gttctgccca tgcacgttct tgcaggctaa
     6661 aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctggctg tcgtgtaacc
     6721 attcacgcaa agaacggcga gttcccggtt cgtgcatttc ctgccaggtt tcccacatgc
     6781 tttgcagcgc gcgcggcgca tcttctgcgg gcgcggtcgg caccgtatat tcgttacgtt
     6841 ccacgccgat gatattcgac accagcacgg tatggtgcgc agtcaccgca cggcccgatt
     6901 cggtgattac cgtcggatgc ggcagaccat tttcttcaca cgcatcgcca atcgcccaga
     6961 tgatgttgtt ggcgtattca ttgaggccat agtttacgga gcagtcagac tgcgaacggg
     7021 tgccttcata gtccacgccc agaccgccgc cgacgtcgaa gcactgaata ttgacgccca
     7081 gcttgtgcag ttccacatag aaacgcgcgg attcacgaac gcccgtggcg atatcgcgaa
     7141 tattcgccat ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg agacgcccgg
     7201 cttcacgcag ggtttcaacc agttgcagta cctgagtcgc agccaggccg aacttcgatt
     7261 tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt gcacgcacgc
     7321 ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg atttctgaca
     7381 tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat gccaggcgga
     7441 tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgggtcatg ccagcatgtg
     7501 ccagtactgc catcaactcg gctttggaac cggcttccag acccagcggt tcgcccgaat
     7561 gaatcaggga ctcaatcacg cggcggtgct ggttaacttt gatcggataa acaaggaagt
     7621 aatcgccgtt atagccgtag gattccctcg cacgtttgaa cgcggcgtta atggaacgca
     7681 aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga ccctgtgctt
     7741 cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc
     7801 acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac caggcaatat
     7861 tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc tgcatggagc
     7921 gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg tcagacatag
     7981 cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag cctggaactg
     8041 cgataacaac ccataagcac atggattttc cagcagtgaa tgctgacgct ccaactgcgc
     8101 aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg tgacagttcg
     8161 gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc ctcagtaact
     8221 gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc aggtatgcaa
     8281 agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca ggttaagcag
     8341 cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac gaacccacaa
     8401 gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt
     8461 tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg caaaagataa
     8521 atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa cagaatgaga
     8581 cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc catccagatg
     8641 ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacctg cagagtcgtg
     8701 gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa ttctctttag
     8761 gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga agggcatcct
     8821 gacaaaattg ctgaccaaat ctctgatgcc gttttagacg cgatcctcga acaggatccg
     8881 aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt tggcggcgaa
     8941 atcaccacca gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt
     9001 ggctatgtgc attccgacat gggctttgac gctaactcct gtgcggttct gagcgctatc
     9061 ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct ggaacagggc
     9121 gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt gctgatgcca
     9181 gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc
     9241 actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta tgacgacggc
     9301 aaaatcgttg gtatcgatgc tgtcgtgctt tccactcagc actctgaaga gatcgaccag
     9361 aaatcgctgc aagaagcggt aatggaagag atcatcaagc cgattctgcc cgctgaatgg
     9421 ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gttttgttat cggtggcccg
     9481 atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg cggcatggca
     9541 cgtcacggtg gcggagcatt ctctggtaaa gatccatcaa aagtggaccg ttccgcagcc
     9601 tacgcagcac gttatgtcgc gaaaaacatc gttgctgctg gcctggccga tcgttgtgaa
     9661 attcaggttt cctacgcaat cggcgtggct gaaccgacct ccattatggt agaaactttc
     9721 ggtactgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt cttcgacctg
     9781 cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa agaaaccgca
     9841 gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg
     9901 ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gctttcaaag gccagcctcg
     9961 cgctggcctt tttcttttac ctcaccgcgg tggataggca ttcacgccgc atccggcaaa
    10021 aagctgcccg cacaataaca tcattcttcc tcaacacgtt ttatcgcaaa ttatcatcac
    10081 tactga
//
LOCUS       DAZOZY010000146        10004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZOZY010000146 DAZOZY010000000
VERSION     DAZOZY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.146"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            177..749
                     /gene="lpfA-O113"
                     /locus_tag="ACHURQ_003107"
     CDS             177..749
                     /gene="lpfA-O113"
                     /locus_tag="ACHURQ_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR0824826.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            797..1528
                     /gene="lpfB-O113"
                     /locus_tag="ACHURQ_003108"
     CDS             797..1528
                     /gene="lpfB-O113"
                     /locus_tag="ACHURQ_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR0824827.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1553..4075
                     /gene="lpfC-O113"
                     /locus_tag="ACHURQ_003109"
     CDS             1553..4075
                     /gene="lpfC-O113"
                     /locus_tag="ACHURQ_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR0824828.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            4086..5159
                     /gene="lpfD-O113"
                     /locus_tag="ACHURQ_003110"
     CDS             4086..5159
                     /gene="lpfD-O113"
                     /locus_tag="ACHURQ_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR0824829.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5407..6447
                     /gene="pstS"
                     /locus_tag="ACHURQ_003111"
     CDS             5407..6447
                     /gene="pstS"
                     /locus_tag="ACHURQ_003111"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR0824830.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            6534..7493
                     /gene="pstC"
                     /locus_tag="ACHURQ_003112"
     CDS             6534..7493
                     /gene="pstC"
                     /locus_tag="ACHURQ_003112"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR0824831.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7493..8383
                     /gene="pstA"
                     /locus_tag="ACHURQ_003113"
     CDS             7493..8383
                     /gene="pstA"
                     /locus_tag="ACHURQ_003113"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR0824832.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8474..9247
                     /gene="pstB"
                     /locus_tag="ACHURQ_003114"
     CDS             8474..9247
                     /gene="pstB"
                     /locus_tag="ACHURQ_003114"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR0824833.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9262..9987
                     /gene="phoU"
                     /locus_tag="ACHURQ_003115"
     CDS             9262..9987
                     /gene="phoU"
                     /locus_tag="ACHURQ_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR0824834.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2588 a         2275 c         2525 g         2616 t
ORIGIN      
        1 attttttact gtttaaataa tatttaaaca ataaatttat attttatttt tgcgtaatta
       61 atattatttt tagataaagc cgtctctttg gtgtactatt cctttgaatc aaaaatacac
      121 ttattaatat aagtgatgat tcatggtaaa ggatatatta tatcaatgga gtttttatga
      181 agcgtaatat tataggcggt gcattcactc tggcatctct aatgctggcc gggcatgcac
      241 tggcagaaga tggtgttgtt cacttcgtcg gtgaaattgt cgacactact tgtgaagtta
      301 cctccgatac agccgatcaa attgtcccac tgggtaaagt cagtaaaaat gcattttcag
      361 gtgtaggtag tctggcgtcg ccacagaagt tcagtattaa acttgaaaat tgcccggcaa
      421 cgtacactca agcagccgtt cgttttgatg gtacagaagc gcctggcggc gacggcgacc
      481 tgaaagtggg tacgccgctt acagcaggca accctggtga ttttaccggt acaggacaag
      541 cgattgcggc aaccggcgtt ggtattcgta tttttaacca gtccgataat tcgctggtta
      601 aactttataa cgactctgct tataccgcta tcgatgctga aggcaaggct gaaatgaagt
      661 ttattgcacg ctatgtggca accaatgcga ccgtaacggc tggtacggcg aacgctgatt
      721 cacaatttac tgtcgaatat aagaaataat ttatcaaggg cctttttatt cagttggctt
      781 tggccaggga gtcgttatgt ccagaaaaag ttgtttaatc aggctcttta aaacaacagt
      841 ttcatgtaag ttatttaccg cgattatact cagcgttttt ttaggccaac cagcattaac
      901 ttatgcaggc gttgtcattg gtggtacgcg ggtggtttac ttaagtaata atgctgataa
      961 atctatttct gtatttagca aagaagaaaa aataccttat ctcatccagg cttgggtaga
     1021 tccatttaat aaagaggata aaagcaaagc gccgtttacc gttattccgc cagtatcacg
     1081 attagaacct tcgcaagaaa aaatattacg cattattcac accaaaggtg tgtcattgcc
     1141 tgatgatcgc gagtcagtct tctggctgaa tattaaaaat attccgccat cagcgagcaa
     1201 caaagcgacg aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc
     1261 agcaaatatc cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg
     1321 gcgaaatctg atagcagaga atcccaaccc aattcatatc agtgtgatgg atgtcatcgt
     1381 tgatggccat gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca cgttaccttt
     1441 acctgctaat tctgcgggag gccaaatgac ctggcgattt attaatgatt acggcgcagt
     1501 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
     1561 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
     1621 ggccgaggtc agtgtattca atccggcgct tctggaaatc gaccatcaat ccggagtcga
     1681 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
     1741 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
     1801 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
     1861 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
     1921 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattcc
     1981 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
     2041 gattaacgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
     2101 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
     2161 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
     2221 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
     2281 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
     2341 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
     2401 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtctgccgt
     2461 tccacaaggt gcttttgaga tcaccgatct caacaccgcc agtacaggtg gcgatttgga
     2521 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     2581 agcgattctt aaacgtgaag gtcagacaga tgttgatgtc agcgtgggtg aattgcgcga
     2641 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     2701 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     2761 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     2821 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaacgatt
     2881 tgacgacaca gacactagct tgcgcttggt tggctatcgt tactccaccg agggctacta
     2941 taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
     3001 ccgacgtagt tgcgtggagg ggacgctaac gcagtcgttg gggagagatt atggcaattt
     3061 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
     3121 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
     3181 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
     3241 catgctctct ttatctgttc ctttattggg ctggtggggt aatagttacg ccacctattc
     3301 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
     3361 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
     3421 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
     3481 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     3541 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     3601 ggccgccggg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     3661 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     3721 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     3781 agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca gagtgttaaa
     3841 tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg caagtttggc
     3901 agattcagga attgtcggtg accgtggcga actttatctt tctggtctac cagaaaaagg
     3961 acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca attattcact
     4021 ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc attaaaagga
     4081 tttttatgaa caagtatatc aaacagtggt gctttgctgt gtttatgctc tcgttaagta
     4141 gtgtagcact tgcagctcct aaaggtatct gtacccctga taatggcgta tttcaccgta
     4201 cgcttgattt ctcgggatat cttattaccg cgaacgaaaa taaggttgga acgacattta
     4261 ataccactgt aactaatgga tcctcatatc ctggacgctg tcattgtgat actggtaatg
     4321 taggagaatt cccctatatc tattatacgt caaaaataaa ccaggcatta acttatgctg
     4381 gggttcactc taatattaat tattatgatc ttaatccgaa ccttgatgtt gggattgcta
     4441 tagatattct tggggttggg tatgtgaatg caccttttga atatcatgct aacaacccgt
     4501 ctggtaatac aaagtacaat tgcaaccgca ttgaaccttt aagtatatcc agtgggggta
     4561 aagcgatagt atatttttat atcaagaaga catttgcagg aaaattgatt attcctgaaa
     4621 caaagatagt gacattgtat ggaacaatta gccgtgacac cccggtggat tactcacagc
     4681 cgatggctga tgtttatatt cggggcgata ttacagctcc gcaaagttgc gaaataaata
     4741 atttacagcc agtttatttt gattttaaag aaataccagc tgcagatttt tcatctgttg
     4801 ttggaagtgc ggtaacaaca cataaaatta ctaaaacagt cactattgag tgtgaaaacc
     4861 tggggatact gaataccgat gatatcagta cgtcttttta tgctaccgaa cccaatacgg
     4921 acaattcaat ggtcgtaact tcaaactcta acgtggggat aaaaatttac gacaaaaata
     4981 ataaggaaat caaagtgaac ggtggtgagt tgccaacaga catgggtaaa tcaacagtgt
     5041 atggtgaaaa atcaggtagt gtaacttttt cagctgctcc tgcgagcctt actggagctc
     5101 gcccagcccc ggggcaattt actgcaacag cgacgataac tgttgagatt gtgcgctaat
     5161 aggtctgaca acgattgttc atcacatcgt tgtcatctca cccatttctt tatcactttt
     5221 aaatgcatca tgacaaaaag atgacatgtg ggattttcat cttcacattt aaaatcagta
     5281 agttatttgt taattccttt ctgtcataaa aatgtcatat tccttacata taactgtcac
     5341 ctgtttgtcc tattttgctt ctcgtagcca acaaacaatg ctttatgaat cctcccagga
     5401 gacattatga aagttatgcg taccaccgtc gcaactgttg tcgccgcgac cttatcgatg
     5461 agcgctttct ctgtgtttgc agaagcaagc ctgacaggtg caggtgcaac cttccctgcg
     5521 ccggtgtatg ccaaatgggc tgacacttac cagaaagaaa tcggtaataa agttaactac
     5581 cagggtatcg gttcttccgg tggcgtaaaa cagattatcg ctaataccgt tgattttggt
     5641 gcctctgacg cgccgctgtc tgacgaaaaa ctggctcagg aaggtctgtt ccagttcccg
     5701 accgtgattg gcggcgtggt gctggcggtt aacattccag ggctgaagtc tggcgaactg
     5761 gtgctggatg gtaaaaccct cggcgacatc tacctgggca aaatcaagaa gtgggatgat
     5821 gaaaccatcg ccaaactgaa tccgggtctg aaactgcctt cacaaaacat tgctgtagta
     5881 cgccgcgcag atggctccgg gacttccttc gtcttcacca gctacctggc gaaagtgaac
     5941 gaagagtgga aaaacaacgt tggtactggc tctaccgtaa aatggccgat cggtctgggc
     6001 ggtaaaggta acgacggtat cgccgcgttc gttcagcgtc tgccgggtgc aattggttat
     6061 gttgaatatg cttacgcgaa gcagaacaac ctggcgtaca ccaaactgat ctccgctgat
     6121 ggtaaaccgg ttagtccgac cgaagaaaac ttcgctaatg cagcaaaagg tgcagactgg
     6181 agcaaaacct tcgctcagga tctgaccaac cagaaaggcg aagatgcatg gcctattacc
     6241 tctaccacgt tcattctgat ccacaaagat cagaagaaac cagaacaagg cacagaagtg
     6301 ctgaaattct tcgactgggc gtacaaaacc ggggctaaac aggcgaacga cctggattac
     6361 gccagcctgc cggatagtgt agttgaacag gttcgcgctg cgtggaagac caatattaaa
     6421 gacagtagcg gtaagccgct gtactaataa aactctaggc cgggtacggt gttttacgcc
     6481 gcatccggca ttacaaaatg actttgtaaa cgcgtttaac tgaagagtaa cttatggctg
     6541 caaccaagcc tgcttttaac ccaccgggta aaaagggcga cataattttc agcgtgctgg
     6601 taaaactggc ggcgctgatt gtgctattga tgttgggtgg cattattgtc tctctgatca
     6661 tctcctcctg gccgagcatt cagaaatttg gtctggcttt cctatggacc aaagagtggg
     6721 atgcaccgaa cgatatctac ggggcgctgg tgccgatcta cggtacgttg gtgacttcgt
     6781 ttatcgcgct gctgatcgcc gtcccggtga gtttcggtat cgccctgttc ctgactgagc
     6841 ttgcgcctgg ctggctgaaa cgcccgctgg gtatcgccat tgagctgctg gcagccattc
     6901 caagtatcgt ttacggcatg tggggcctgt ttatctttgc gccgctgttc gccgtttact
     6961 ttcaggagcc ggtcggcaat atcatgtcga atatcccgat tgttggcgcg ctgttctctg
     7021 gccccgcatt tggtatcggt atcctcgcgg caggcgtgat cctcgccatc atgattattc
     7081 cgtacattgc ggcggtaatg cgtgatgtgt tcgaacaaac cccggtgatg atgaaagagt
     7141 cggcctacgg tattggctgc accacctggg aagttatctg gcgtatcgtt cttccgttca
     7201 ccaaaaatgg tgttatcggc ggcatcatgc tggggctggg ccgcgcgctc ggtgaaacca
     7261 tggcggtgac ctttatcatc ggtaacacct accagctcga cagcgcctcg ctgtacatgc
     7321 cgggcaatag tatcacctct gcgttggcga acgaatttgc ggaagcggaa tccggtctgc
     7381 acgttgccgc actgatggaa ctgggcctga tcctgtttgt gattaccttc atcgtcctcg
     7441 ccgcatcgaa gtttatgatt atgcgcctgg ctaagaatga gggggcacgc taatggctat
     7501 ggttgaaatg caaaccactg cggcgctggc tgaatctcgc cgcaaaatgc aggcgcgtcg
     7561 ccgcctcaaa aaccgtattg cgctgacgct ctcgatggcg acgatggcct tcggcctgtt
     7621 ctggctgatc tggattttaa tgtccaccat cactcgcggt atcgacggta tgtcgctggc
     7681 gctgttcact gaaatgacgc cgccgcccaa tacggaaggt ggtggtctgg cgaacgccct
     7741 ggcgggtagc gggctgttaa ttttgtgggc cacggtattc ggtacgccgc tgggcattat
     7801 ggcggggatt tatctggcgg aatatggtcg taaatcctgg ctggcagaag tgattcgctt
     7861 cattaacgac attctgctct ccgcgccgtc gattgtggtt ggtctgtttg tctacaccat
     7921 cgtggtggca cagatggagc acttctccgg ctgggcgggc gtaattgcgc tggcgctgtt
     7981 gcaggtgccg atcgttatcc gcaccaccga gaacatgctg aaactggtgc cggacagcct
     8041 gcgtgaagcg gcttatgcgc tgggtacacc gaagtggaag atgatctccg cgattacgct
     8101 gaaagcgtcg gtgtccggga ttatgaccgg tatcctgctg gcgattgccc gtattgccgg
     8161 tgaaaccgcg ccgctgctgt ttaccgcgct ctccaaccag ttctggagca cggacatgat
     8221 gcagccgatc gccaacctgc cggtgacgat ctttaagttt gcgatgagcc cgtttgcgga
     8281 atggcaacaa ctggcctggg ccggggtatt gatcattacc ctgtgcgtcc tgctgctgaa
     8341 cattctggcg cgcgttgttt ttgcgaagaa taaacacggt taacactttg ccggatgcgg
     8401 cgtgaacgcc ttacccggcc tacggtaagc ctgattagcg aaaagcatca ggtacgatga
     8461 ggaaaagatt gcaatgagta tggttgaaac tgccccgagt aaaattcagg ttcgtaattt
     8521 gaacttctac tacggcaaat tccatgccct gaaaaacatc aacctggata tcgctaaaaa
     8581 ccaggtaacg gcgtttatcg ggccgtccgg ctgcggtaaa tcgacgctgc tgcgtacctt
     8641 caacaaaatg tttgaactgt atccggagca gcgcgcagaa ggtgaaattc tgcttgatgg
     8701 cgacaacatc ctgaccaact ctcaggatat cgcactgctg cgtgcgaaag tgggcatggt
     8761 gttccagaaa ccgacgccgt tcccgatgtc catctacgac aacatcgctt ttggtgttcg
     8821 tctgtttgag aagctctccc gtgccgacat ggacgagcgc gtgcagtggg cattgaccaa
     8881 agccgcattg tggaacgaaa ccaaagataa attgcaccag agcggttact ctctctctgg
     8941 tggtcagcaa cagcgtctgt gtattgcgcg tggtatcgcc attcgcccgg aagtgctgct
     9001 gctcgacgaa ccgtgttcgg cgctcgaccc catctctacc gggcgtattg aagagctgat
     9061 caccgaactg aagcaggatt acaccgtggt gatcgttacc cacaacatgc agcaggctgc
     9121 gcgttgttcc gaccacacgg cgtttatgta cctgggcgaa ttgattgagt tcagcaacac
     9181 ggacgatctg ttcaccaagc cagcgaagaa acaaacagaa gactacatca ccggtcgtta
     9241 cggttgattc aggagtgcgt tatggacagt ctcaatctta ataaacatat ttccggccag
     9301 ttcaacgccg aactggaaag tatccgcacg caggtgatga ccatgggcgg catggtggag
     9361 cagcagcttt ctgatgcaat caccgcgatg cataaccagg acagcgatct ggcgaagcgc
     9421 gtgatcgaag gcgacaagaa cgtcaacatg atggaagtgg cgatcgatga agcctgcgtg
     9481 cgcattatcg ccaaacgtca gccgacggcg agcgacctgc gactggttat ggtgatcagt
     9541 aaaaccattg ccgagctgga gcgtattggc gacgtggcgg acaaaatctg ccgtactgcg
     9601 ctggagaaat tctcccagca gcatcagccg ttgctggtaa gtctggagtc gctgggccgt
     9661 cataccatcc agatgctgca cgacgtgctg gacgcgttcg cgcggatgga cattgacgaa
     9721 gcggtacgta tttatcgtga agataaaaaa gtcgatcagg aatacgaagg tattgttcgt
     9781 caactgatga cctacatgat ggaagattcg cgtaccattc cgagcgtact tactgcgctg
     9841 ttctgcgcgc gttctatcga acgtattggc gaccgctgcc agaatatttg tgagtttatc
     9901 ttctactacg tgaaggggca ggatttccgt catgtaggcg gtgatgagct ggataaactg
     9961 ctggcgggaa aagatagcga caaataattc accagacaaa tccc
//
LOCUS       DAZOZY010000147         9855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZOZY010000147 DAZOZY010000000
VERSION     DAZOZY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.147"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHURQ_003116"
     CDS             <1..347
                     /locus_tag="ACHURQ_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR0824835.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHURQ_003117"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHURQ_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR0824836.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHURQ_003118"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHURQ_003118"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0824837.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2117..3529
                     /gene="cycA"
                     /locus_tag="ACHURQ_003119"
                     /gene_synonym="dagA"
     CDS             2117..3529
                     /gene="cycA"
                     /locus_tag="ACHURQ_003119"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR0824838.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHURQ_003120"
     CDS             complement(3574..4236)
                     /gene="ytfE"
                     /locus_tag="ACHURQ_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR0824839.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4344..5309)
                     /locus_tag="ACHURQ_003121"
     CDS             complement(4344..5309)
                     /locus_tag="ACHURQ_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0824840.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHURQ_003122"
     CDS             complement(5417..6277)
                     /gene="qorB"
                     /locus_tag="ACHURQ_003122"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0824841.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6366..6746
                     /locus_tag="ACHURQ_003123"
     CDS             6366..6746
                     /locus_tag="ACHURQ_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR0824842.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHURQ_003124"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHURQ_003124"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR0824843.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHURQ_003125"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHURQ_003125"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR0824844.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
BASE COUNT         2287 a         2487 c         2558 g         2523 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat gagtcccgat
     2041 acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac acagacaggt
     2101 acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc aggctccggc
     2161 tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg ccattggcgg
     2221 tgccattggt acggggttgt ttatggggtc cggcaaaacg attagccttg ccgggccgtc
     2281 gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc gggcaatggg
     2341 ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt ccgatttact
     2401 cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg ttgtaaccgg
     2461 tatggcagac gtggtggcga tcactgctta tgctcagttc tggttccccg atctctccga
     2521 ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg ccaccgtgaa
     2581 aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca ttgtgtcgct
     2641 gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg gtgtggaagc
     2701 gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa gtggcttctt
     2761 tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag gtacaaccgc
     2821 tgcggaaacc aaagatccgg agaaatcact gccacgcgcg attaactcca ttccgatccg
     2881 tatcattatg ttctacgtct tcgcgctgat tgtgattatg tccgtgacgc cgtggagttc
     2941 ggtagtcccg gagaagagcc cgtttgttga actgtttgtg ttggtaggtt tgcctgcggc
     3001 tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta acagcggcgt
     3061 cttctctacc agccgtatgc tgtttggtct ggcgcaggaa ggtgtggcac cgaaagcgtt
     3121 cgctaaactt tctaagcgcg cagtacccgc gaaagggttg accttctcgt gtatctgtct
     3181 gctcggcggc gtggtgatgt tgtatgtgaa tcctagcgtg attggcgcgt tcacgatgat
     3241 tacaaccgtt tccgcgattc tgtttatgtt cgtctggaca attatccttt gctcgtacct
     3301 ggtttaccgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga tgccgctcgg
     3361 caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtggtcgtgt tgctgacact
     3421 ggaagatgac actcgccagg cgctgttggt caccccgctg tggtttatcg cactggggct
     3481 gggctggttg tttattggta agaagcgggc tgctgaactg cggaaataac cgcattatca
     3541 tgctggatgg cgcaatgcca tccagctttt agattactca cccgccagcg cgcgtgggaa
     3601 cagtacattg ttttccaggc tgatgtgatc catcaggtca tcaatcagtt cattaatacc
     3661 gttatacatc gctttccagg tggtgcaggc ttccggcggc ggtgtgacgt tattggtggt
     3721 gtgtttaatc acttccagca gttcgcccgc ttcatcgtgc tcgctttcca tcacgctgat
     3781 tggtcccatt gcctggctgc ccatgccttg tttgatcatc gggaagagga tctgctcttc
     3841 tttcatcatg tggctggata gctcttcatg cagcatagtc aggtattttg tcagcccttt
     3901 tggcacgctc ggtttgtcgg cgtgaacgcg ctcgacttta gtcgcttgca ggatcagctc
     3961 cggcagttgc tcgcggtgac gatcgtggta gcgcacgatg atatggtcaa tgatttctgc
     4021 cagcggggcg ctacgccagt ctttctcaat cggttgttca gcaagctttg ccagttcagc
     4081 ttcaatgacc tcaacatcca gttctttacg tgccgccgcg cgcgccagcg tttgcttacc
     4141 gccacagcag taatccatat catatttacg aaacagagct gaagcgcgag gaatagagag
     4201 cgccagttca cctaaaggtt ggtcgcgata agccatagct gatacctcat tcttaataat
     4261 ataagatgta ttttaaatgc atctttaagg caaaagctat aacccttacg tagcagaggc
     4321 ttatttaact cactgccagc gtgtcaactt tctgagacag attcagttaa aggtttttct
     4381 ggttttgctc tgaccgcaat gaccacgcca atcactaaca gcgcgatacc gctcagcgtc
     4441 attagcggcg gcatttgctg gcggagtaaa aaggtgtaca gcaaacctgc cagcgtttcg
     4501 aaaacaatca gcggcccgag aatcactgtc ggtaatcgct ggctggcgac gttccagcag
     4561 agtgcgccaa cccatgagca aagcacggct atcgcaacca tcagactaat aaacaccagc
     4621 ggacgggggc caaaaggtaa ggagaagtcc ggcgtttgta tattcagcca gtaacaggcg
     4681 acgagatagc cgatgagaga aaccggcagt gtgaccagcg cctgcgccgt tgcccacatc
     4741 atcggatgtt tgtcgggatt ttcccgcagc cagcgggcgt tgcgcagggc ataccacgcc
     4801 cagcagacca cggaaactaa cgccagcacg atgccagagg tataacgtgc ccagtcaaaa
     4861 tcggggagtc cgtggtttaa ctcagcaata ttcacacacg ccaggccgat gccaatacaa
     4921 accagtgccg gggcgagttt tccccatgcg agtttgccgt cgcgctggct ataaagcaga
     4981 ttggcaaaaa cgggaatcac caccggcagg gtgccgataa tcatcgtgga aacaggcgcg
     5041 ccagtacgtt gaatggcact ggcaaggcag aagtaataga taaggttgcc catcatggtg
     5101 agcatcaagg cggtaagcca gtcgcgacgc gccaactgac gcagacgcac gcgtcccagc
     5161 caggcaatgg gcagcgcaat taaccctaac gccagataac gccccatcga ctgcaacatc
     5221 gccgggtatt ccggtacgat caacggcccg acaaaaataa gcccccacat caaccctgct
     5281 aacagggcgt acagcacgcc gctaatcatt actggcatcc attggtctgt cagaagaaag
     5341 ttcaggaagc agacatgatt gctcccggca tcccgtcatt atcagagagg atgcgggagg
     5401 atgccacttt aattaactag ttattaacat taaaaagatg gcttacgctt tcggctaacg
     5461 ttgtcgttgg gcggccaatc aatttgctaa gcgttttgct gttatcaaac agaccacctt
     5521 tcgatgcgcc aacgtcagaa tccgccagca tatccgccag tccgtcgggc agtccgacgc
     5581 ttttcagtgc cgcggcgaaa tcggcttcgc tcagattttg ataggtaacc tgtttgccgc
     5641 tctgtttggt cagctctgcc gctaactgtg tcaacgtcca ggcactatcg cccgccagtt
     5701 cgtaaacctt gccttcgtga ccggcttcgc taatcacgcg tgccgcagct gccgcataat
     5761 ctgcccgcgt tgctgaggcg attttgccat cgcccgccgc accgataaat acgccgtgtt
     5821 ccagtgctgc cggggcgctg gcgaggtagt tttcgctgta ccagccgttg cgcagcaggg
     5881 tgtaaacgat gccagaatca gccagcattt tctccgtctc aatgtgctca tcggcgaggc
     5941 cgagcgggga ggtatctgca tgtagcaggc tggtataagc gataaatttc acgccagccg
     6001 cctttgcggc attaataaca ttacgatgct gcggggcacg ttgacccact tcgctggaag
     6061 agatcagcag tagtttttcc actccctgaa gtgcagatgt cagtgcggct tcatcgccgt
     6121 agtcagcctg acgcacggta atgccttgtg ctgctagagc ctgggctttt gccggattac
     6181 gaacgatagc cactatttgg ctggcaggaa ccgttttcat caaggattca ataatatagt
     6241 gaccaagttg gccagtggca ccagtaatag cgatcatggg aagtctccat cgttttgtct
     6301 tgtgtttaaa tacgctaaca cctaaactta cttttagtaa gtacgtacaa aaaggtaagt
     6361 atgaaatgag tcaggttagc ctgtcgcaac aactgaaaga gggtaatctc tttgcggaac
     6421 agtgcccgtc gcgcgaggtg ttgaaacacg tcaccagccg ttggggggtg ttgattctgg
     6481 tggcgctacg cgaaggtact catcgcttta gcgacctgcg gcgcaaaatc ggcggggtga
     6541 gcgaaaagat gcttgcgcag tcgttacagg cgttagaaca ggatggtttt cttaaccgta
     6601 tcgcgtatcc ggtggtgcca ccgcatgttg aatacagcct cacgccgctg ggcgagcagg
     6661 tgagcgaaaa ggttgctgca ctggcggact ggattgagtt gaatttgccc gaggtgttgg
     6721 cggtgcggga tgaacgtgcg gcataactgc cggatgcgcg gcctacggtg tatgacttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc gattgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tactgccctt tctctttaat aaacgctgcg
     6961 gctttatttg acggggaagt ttcgaaacgg atatccagtt tcttatcggc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gtattaatca tctggcactc gccgtcataa cgggcgggct gggtaacatc aatctgataa
     7321 ttcacaccat caataacatc aaagttataa gtgcggaaac catcccagtt gatgagtgac
     7381 tgtggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cacgacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgcgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggcac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt atctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaattcatga
     8461 ttgccgagtg taccgaccgt ataatccagc gtattcagcg ccttataaac cggatgaaca
     8521 tcacctgttt ttaatccttt cgccgacatg taatctgcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgcaca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgtaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggctgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat ccggggttca
     9721 gagtgtctat ttactaaatt cagatggcag aaacagtgta tttcctgatt ctgccatcct
     9781 gatttctccc aacctaaaaa gttataaata aaaagagatt gtatttaaag tgcaaaaatt
     9841 caattgctaa taagt
//
LOCUS       DAZOZY010000148         9706 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZOZY010000148 DAZOZY010000000
VERSION     DAZOZY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9706
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.148"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..145)
                     /locus_tag="ACHURQ_003126"
     CDS             complement(<1..145)
                     /locus_tag="ACHURQ_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824845.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFS"
     gene            223..1317
                     /gene="ddlA"
                     /locus_tag="ACHURQ_003127"
     CDS             223..1317
                     /gene="ddlA"
                     /locus_tag="ACHURQ_003127"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR0824846.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1341..1553)
                     /locus_tag="ACHURQ_003128"
     CDS             complement(1341..1553)
                     /locus_tag="ACHURQ_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR0824847.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1813..2121
                     /locus_tag="ACHURQ_003129"
     CDS             1813..2121
                     /locus_tag="ACHURQ_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR0824848.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2180..3274)
                     /gene="yaiW"
                     /locus_tag="ACHURQ_003130"
     CDS             complement(2180..3274)
                     /gene="yaiW"
                     /locus_tag="ACHURQ_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR0824849.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3287..4507)
                     /gene="sbmA"
                     /locus_tag="ACHURQ_003131"
     CDS             complement(3287..4507)
                     /gene="sbmA"
                     /locus_tag="ACHURQ_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR0824850.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4859..6016
                     /gene="ampH"
                     /locus_tag="ACHURQ_003132"
     CDS             4859..6016
                     /gene="ampH"
                     /locus_tag="ACHURQ_003132"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR0824851.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6017..6640)
                     /gene="iprA"
                     /locus_tag="ACHURQ_003133"
     CDS             complement(6017..6640)
                     /gene="iprA"
                     /locus_tag="ACHURQ_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR0824852.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6728..9616)
                     /locus_tag="ACHURQ_003134"
     CDS             complement(6728..9616)
                     /locus_tag="ACHURQ_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR0824853.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNNKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2492 a         2403 c         2340 g         2471 t
ORIGIN      
        1 gagaaaaaaa ctcaccgcat tgccatttaa gccaggaata aattagcttc ccgttcatca
       61 tttttgtgat ctcgcgcaca tctttgagat aacgcacttt cttcaccagt acgttgcacg
      121 ctttgtcagc agaagattgc aacaatcttt cagaaggccg cgaatccccc gttttgtcgt
      181 ggtaatgtat gcactttgtg ttagacggga taggttttta agatggaaaa actgcgggta
      241 ggaatcgttt ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc ggcaaaaaac
      301 attgtcgatg ccattgataa aagtcgcttc gacgttgtgc tgctgggcat tgataaacaa
      361 gggcaatggc acgtcagcga tgccagcaat tatctgctaa atgcagacga tcctgcccat
      421 attgcgttgc gcccttcggc gaccagcctt gcgcaggtgc caggtaaaca tgagcatcag
      481 cttatcgacg cgcaaaacgg tcagccgttg ccgacggtgg atgtcatttt cccgattgtc
      541 cacggtacgc tgggcgaaga tggttccttg cagggaatgc tgcgggtcgc caatttaccg
      601 tttgtaggtt ctgatgttct ggcttcagca gcctgtatgg ataaagatgt caccaaacgt
      661 ctgctacgcg atgccgggct gaacattgcg ccatttatta ccctgacgcg cgctaatcgt
      721 cacaacatca gttttgccga agtggagtct aaactggggt taccactgtt tgtaaaaccg
      781 gctaatcagg gctcttctgt tggtgtcagc aaagtaacca gtgaagaaca gtacgcaatt
      841 gccgtcgatc tggcgttcga gttcgatcat aaagtgatcg ttgagcaagg gatcaaaggt
      901 cgtgagatcg aatgcgcagt tctgggcaac gacaatccgc aagccagcac ctgtggcgag
      961 atcgtactca ccagcgattt ctatgcctac gacaccaagt acattgacga agatggcgcg
     1021 aaagtggtag ttccggcagc cattgcacca gagatcaacg ataagatccg ggcgattgcc
     1081 gttcaggctt atcagacgtt gggatgcgca ggcatggcgc gtgtagacgt gtttttaacc
     1141 ccagagaacg aagtggtgat caacgagatc aacacactgc ctggcttcac caacatcagt
     1201 atgtatccga agctgtggca agccagcggt ctgggttaca ccgatctgat cacacgtttg
     1261 attgagctgg cgctggagcg tcacgctgcg gataacgcat tgaaaaccac aatgtaatat
     1321 tttaacgccc gataatattc ttattgggcg ttttcagttt cttcatcgcg acgacgaata
     1381 atcaaccccg ccagccagaa actaatcacc cacgtcacca gaccgacggc ataggtctgc
     1441 caaccttttg cttcaaatcc cagtaacccc accacgccat taagaatgaa tataaggcca
     1501 atggcgaacg catagtagtg ccagtcacgg cggattttta caggcagatt catggcagct
     1561 ccgttaacga aaaacaacat ccttgcatat tctgtaacaa ccgtctgtgg tgtagacaac
     1621 attttcgtaa ttgttaataa atttaactta aggttgccat tttgtaactc acaacgaaga
     1681 ttcacgcttc gggaatattc ccaacatcaa tttaccatga ttctgatatt gacaatctaa
     1741 atgacctgtc agacttgcca taagccgctg gtatcacacc agaagagaag cagatgactt
     1801 ccggaggttt ttatggctga tttcaccctg tcaaaatcgc tgtttagcgg aaaatatcgc
     1861 aatgcctctt caacgcctgg caacattgcc tatgcgttgt ttgtgctgtt ttgcttttgg
     1921 gctggggcgc aattgctgaa cctgttagtg catgcgcccg gcgtctatga gcgtttaatg
     1981 caggtccagg aaacaggtcg cccacgggtg gaaattggtt taggtgtcgg caccattttc
     2041 gggctgatcc cgtttttagt aggctgcctc atttttgcag tggtggcgct atggctgcac
     2101 tggcgacatc gccgccagta agatttacgt tgccagatac ggcctaaaac gccttatctg
     2161 gcatgacatt cctgtttgat cactgtttca tacaacgcgc ccgccgttcg tctacgcgct
     2221 tcgcaaacca ggccgtagtc aggttgcggg tgattttcgg gctttccagt tgaatgccag
     2281 gcaacatttc gcggggtagt gatttacccg ttttcgcttc ggcaagttga taaactttct
     2341 tgtacagcgc cgtctcctca aaagagaagc tgtcaccttt ctctaactga cggcgaattt
     2401 cgctgtcgct catccccagt tttcccgcca gtttgcgcgt tgccagttcg gttttcccgg
     2461 gttctttact gtcgtaacga attaaatcac cgtccagtgc cagcttcacg ccgctggcct
     2521 tactgactgc gttctgaaac gcggcattac ggctggcgta ccagccagcg ttaaaatcag
     2581 caaagcggta tatcggtgcg ctatagctgg cgggataatt cagtaaatgg taagtaccaa
     2641 accacaaccc gccgcggcga ctgaagactt cctgacgaac tgtaccgtcc attttccacg
     2701 gatacccttt ggtatgctgt tcggcaaaag caatgctgac ctgcatcggc ccaccggtgc
     2761 gcaccggatt aagcgaacca aacagcgtct gccccattgg taccatgctg atcagatcgt
     2821 caaaaatcgc gctcaattgc ttttctgtac gcaccgaatc taaccgttcg ctataacttt
     2881 tgccgtttgg cgatttgatt tttagcgcgg tatgaaccag aaaagcagga atgtgcatcc
     2941 gttcagcacg acggtcaatc tcttgccagg cgatcttgct taagcccgga acggccggat
     3001 ctgcctgata atttgactcc tgctgcgcca ccgccagcac cgagcagacg ttttccagcg
     3061 tcggtgccag cccctgactt tcaaaggtga tggcgagatc tttcgcccag gcatcgcggt
     3121 ctttcacgct cgcaggcatc ttctgccgca cgacgctcgc tacatctatc gctttttcgc
     3181 cctttttcaa cggttgcgga gcctgactac tacaccccgc taaaaccaga accgccaaca
     3241 atgacagcga agatagatga ttgacgcgcg acattacttc tcctttttag ctcaaggtat
     3301 gggttacttc ctgaattttg tcaccatcca gctcatgttc aaagctgcgc agacgtttgt
     3361 agatagacat caactcaacc agtgtggtcc atgagttaat caggtactgg aaagcaccgc
     3421 gaacctgacc aaaaacgttg gtaatctgcg tcatcaggcc gagcgtaatc gtaccggcaa
     3481 caattgacgg aaacagcaag aacaaaccga aaacgttatc gacctgcaaa tagagaatgc
     3541 gggcgatgtt gaaatacata tagtgaaaat agaggcgaaa atagtttttc cgtacggcgc
     3601 taaacagctc gcgtaccgta ggcggcgtcg cgcgcgtggc atcgtcttca ccataaacca
     3661 gctctttacg gtaggcggcc tctactcgct ggtttttaaa ctccagcccc ggcagtttga
     3721 tccctaccac tgccagccat ccggtcccca tcagcgacca gacgatagcg gcaatcacca
     3781 gaccatacgg aatgtgcccg ataatcggca gctccggcac atgcgcggag agcgttacca
     3841 gcaccggcag gaaggcgatc aacgtcatga tggcgttgat aaaactgacg cccatattct
     3901 ccagcgttga agcaaaacgc atggtgtctt cctgcacacg ctgtgcggca ccttcgatat
     3961 gacgcagttg ttgccagttc gccatgtaat attcgttcat cgccgtacgc cagcggaaca
     4021 catagtgact gacaaagaag ttgttcagta cactgatcac caccgcgatc agcgcaatcc
     4081 ccagaaagac gcccacttcg cggtaaaatt gttcgatagt gactttatgc ggcgaactta
     4141 gcgcggtttg aatcaaatca tagaacggtg cataccaggc gttgacggcg accccgactt
     4201 ccaccaaaaa ccaggtgacg aagatgatca gtgcagtacc gagtattgac cagtattgcc
     4261 aacggtgcgg gctgtagata aaccagaaaa atgcaaaaag tcctacgcaa acaatgtagt
     4321 aagcgtaaaa aatcaggaaa tccaacgacc agaaacgcgc ggcgctaatc gggatctgcc
     4381 cggaagcgcc ggtgatacgc gccacccagt ctcccccacc ggcttgccag aagataacgg
     4441 caatcaatgc ccaaacaaag gccgagagaa aaaacgctcc cggctttggg aaaaaagact
     4501 taaacatatg cactcctgct aacttcttat cgttttaaca actgctgtgt attaaccgca
     4561 tgaccactac aaagcgtcag ccaaatgaaa ccgcccattg tgacccatag caaggcgctt
     4621 agttcgccag cctgaccata agaattgttt cgacggattt cacaaaccaa agattgttta
     4681 cataagcgga ttaacacgcg caggatcaca aatgcaggca ttggtgagca ggaaaaagat
     4741 cgtgtttacg cgccgttacg tttttaacac tattattgca ctaattcgcc ctttgcaatc
     4801 catcaatgag tagtataaat acgctcagtt accttcattc aatctatgga caccaccgtt
     4861 gaaacgtagt ctgctttttt ctgccgtgct gtgtgcggcg tcattgacct ccgtccatgc
     4921 ggcgcagccg atcactgaac cggagtttgc ctctgatatt gtcgatcgtt atgccgatca
     4981 tattttttac ggcagcggtg caacggggat ggcgctggtt gttatcgacg gtaaccagcg
     5041 cgtctttcgt agttatggcg aaacgcgacc tggtaataac gttcgcccgc agctggattc
     5101 ggtcgtgcgt attgcttccc tcaccaaact aatgaccagt gaaatgctgg tgaaattgct
     5161 cgaccagggg accgtaaagt taaacgatcc gctaagtaaa tatgctccgc caggcgcacg
     5221 cgtgccgacc tacaatggga cgcccattac cctagtgaat ctggcaaccc ataccagcgc
     5281 cctgccccgt gaacagcccg gtggcgcggc acatcgtccg gtgtttgtct ggccaacgcg
     5341 cgagcaacgc tggaaatacc tttctacggc gaagctgaaa gccgcgccag gcagtcaggc
     5401 ggcgtactct aaccttgcgt tcgacctgct ggcagatgca ctggcgaatg cgtccggcaa
     5461 gccttatacc cagttgtttg aagagcagat tacccgtcca ttggggatga aagacaccac
     5521 ctacaccccc tcaccggatc agtgccgccg tttaatggtt gctgagcgcg gtgccagtcc
     5581 gtgcaataac acgctggcgg caattggcag cggcggcgtc tattccacgc ctggcgatat
     5641 gatgcgctgg atgcagcagt atttgtcatc tgatttttat cagcgcagta accaggcaga
     5701 tcgcatgcag acgttgattt atcagcgcgc gcagtttacg aaagtgattg gcatggatgt
     5761 ccccggcaaa gcggatgcgc ttggtttagg ctgggtatat atggccccga aagagggtcg
     5821 tccggggatt attcagaaga caggcggtgg tggcggtttc attacctata tggcgatgat
     5881 cccgcagaag aatattggcg cgtttgtggt ggtcactcgt tcgccgttga cgcgctttaa
     5941 aaatatgagc gatggcatca atgatctggt gaccgagcta agcgggaata aaccgttggt
     6001 tatccccgca tcctgattaa tactctgaag gcaaacggtt gatagcgacc agtttgcctt
     6061 tattcatttc gatatagccg cctttacgca aagcagcgag aacttctgcg acgacagaac
     6121 gcgatatgcg tgtacgttga tggatataat tcatcacacc aatacgtgat cgcaattctt
     6181 cattccagtc aatcattgat aataatgttg cgcggatttg ttcgtaggaa ttatgcccaa
     6241 tgagctgcac gtcgcgtaat tccagaattc tattttgcca ggctaaccag taaaaagcgt
     6301 ctcgccagag ttgattttgt tcaataagcg taatggtttg tttggctggt agatgatatc
     6361 ccgtacaatt tccttctgat attaatttgt atggtatatc gtttttcatt aaaccatcag
     6421 ccagccccat aatataaggg gcctgggtaa taccgataag tacgttttct tctctacgca
     6481 gagagataac tccctctaaa ataacgaaag tatcttcgtt attgacatca ctggaaaata
     6541 taacttgctt ttcattatta aactcgaagc gcgtaccgta tctggacaaa catttatcga
     6601 gcttaccaaa ttcctgaaga ggtttaacta cagataacat ttgcgcgtcc tttgcagtaa
     6661 tgcccgtcaa atccttgacg ggcattattt agattaaatt accagtattt cttcggagtg
     6721 aagaatatta ccaggtatat ttaacaccca cgttcgcgga ccagtcttga tctacgtcac
     6781 caccaccgag gtagttagca tcggtatagg cgctgaagtt cttggtgaag ctgaactgag
     6841 tacccagacc aacacgtacc gcagaccctt cagtaccgtt atcgatggaa tcaccgttca
     6901 catcgttatc gttgttagag tcgtcgtaga cgtaagccag tttgaagtac ggagtcagag
     6961 cctgatcttc gctgtaggtg aaggtataac ctgcatctac acccagttca taacgcatgc
     7021 tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag ctggtagtca tcaccagact
     7081 ggaacagacc agaaacgctg ccgtaaggag tcacgtaacc agcatcaccc agtttgaagt
     7141 cgtaaccggc tttcaagccg aagccccaag cgtcggagtt ggtgctaccg tcaacgtaag
     7201 taccgttgct catggttgca gacaggtcgt tgttgaagtg agagtagctc aagctaccat
     7261 caacaaagac gttgttcgcg aagtgagcag aagagtagat gtaggcagtc tggctgtctt
     7321 gatctacctg accagaacgg tcattcatgt cacctttagc gaagcctgca gccgcaccga
     7381 cgatccactt agcgttgtta ccgtcaactt tggtatcaac accgaccatg atgccgttaa
     7441 catcctgatc atagttgatg gtgccgttgt cgccgttgaa gttaccaccg aagtagctta
     7501 cccatgcgcc gccgttatca gccaggccat gacgagagtt ggtcagacga gtaccaacgg
     7561 tgtcttgttc caggttccag atattggtgt tcgcagacgg gatgctcagc gccatgttag
     7621 cgtagtcggt cagctccatc tgttgcagaa caacggtgtt accgcgctgt tcagcctgat
     7681 aggtgtatgc acccaggtca gctttgttag cagcagagaa ggtcgcgttg gttttggtat
     7741 catcgatata gatgagctgc ttacctgcgt aatcagcaat cgcaccagcg ccagtggagt
     7801 tgtcgatacg aactttgttg ttaccggttg cagctacaac gttgtctgca gagctgttgt
     7861 caacttcgtc gccattgtta atgtcgttgt taccattaac ggtcaagtga ccatcagagt
     7921 tcatcgcaat aacaccataa ccgtagttag ctttgctggt gtcgttggta cgatcgttga
     7981 cgaggtctgc attcaggatg taatcattac tatgaatgtc aaacacacct gcgtaaacat
     8041 taccgttgtt atctttaacg ttgccactgg tcagttccag agtatcagtt gcaaatacgc
     8101 tggagatgtc attcatgttg gtgtttacat ccagcacacc accgttagta acggtaatgg
     8161 tattggcata cagcggtgca tatgcccaag ctgaagcagt atcttcaccg aagttaacgg
     8221 ttgagaaggt atcaatggtc aggtgatcgg tgttaacttc accgttaact tccattgaag
     8281 aaccaccgtt cagagtgatg gtgtcagaag tcagagaaga atctgcactc acgttaacct
     8341 gagaaccgct gttgatcgcc agggtatcga tcagggaagt tttcgtggtt tcccaatcag
     8401 aaccgttgtt cagaacaaca tcgaatacgc cgctctggaa tgcgttagcg ttatcaacac
     8461 gattccagcc atcttcagag taagaagcgt acgggttcag gctgtttggt gcaacgtcgt
     8521 agaaggcttc gtcagcaaca acaacgtcat aatcaatatt cgctgaacca acccatttgg
     8581 tgttgttcag agtcaggttc aggtgatcga cgttctgatc gtcagttgcc caaccgccat
     8641 gagtgtaagc agtggtagag ttgtcaatca ggtgacccgt cgcgtcaaag ttagcgttcc
     8701 aggtactgtc aaatacaacg tcaccaagca actgagagtt gttgaacgta acgttgttga
     8761 ccatacgata atcagaagtg tcatcatcga tgaaggctaa cgcaacatca tcagcaggag
     8821 atgcgccatt gccgtagtca cttggttcaa cagaattgcc gaaatgacca cgatctgatt
     8881 gttcgtcttc agtccatgaa ccagaagtta cagtagagtc attaacaacg atgttgttgt
     8941 cccactgatg accttcggta ttgtaggtcg cattaccgag gtcgtgttga accaaagtaa
     9001 tacctgcaac atgggagttg ttgctgatgt tgatgttact ttcaacatcc agagtcacag
     9061 cggtaccaag accagcggtt accaaacgat cataatctgt cttagatgtt ttaccgtctg
     9121 catccagata agtatcggtg aagtagaagg cgtcaaaatc atcgtcaata gtagaattag
     9181 cgatgttcag agtgaagatg tcgccatcaa cccagtcgtg gccagtataa tcgctaccgt
     9241 tggtccagac gtagtcgcca taaccaacct ggtaagatgt aaccatacca tgaacaattg
     9301 agttggagat gttcagtgta ttaccggtat ctgtgccata atcgtggttc agataataag
     9361 tagaaattac accatttaca gtgtcgccgc tgaaagtggt atcaaagtaa ccatcagctg
     9421 cgtggtcata tacataacca ccgtaagtca gataatcgtg atcaatttta gtgctgtcac
     9481 cccaattgta gatggtggct gcgtcatcgt gatacccgaa ggtgttataa gtagctgcat
     9541 tagcctgaga ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct
     9601 ttttccagga gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta
     9661 tcaatgcata gaaaacatat tatgtttcgc gctaacgatt atgctg
//
LOCUS       DAZOZY010000149         9674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZOZY010000149 DAZOZY010000000
VERSION     DAZOZY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.150"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHURQ_003135"
     CDS             <1..1710
                     /locus_tag="ACHURQ_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR0824854.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHURQ_003136"
     CDS             1711..3360
                     /locus_tag="ACHURQ_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR0824855.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHURQ_003137"
     CDS             3365..4141
                     /locus_tag="ACHURQ_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR0824856.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHURQ_003138"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHURQ_003138"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR0824857.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHURQ_003139"
     CDS             complement(5430..6752)
                     /locus_tag="ACHURQ_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR0824858.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHURQ_003140"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHURQ_003140"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR0824859.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHURQ_003141"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHURQ_003141"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR0824860.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHURQ_003142"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHURQ_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR0824861.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2484 c         2605 g         2231 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgatgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAZOZY010000150         9645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZOZY010000150 DAZOZY010000000
VERSION     DAZOZY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.151"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHURQ_003143"
     CDS             complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHURQ_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR0824862.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHURQ_003144"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHURQ_003144"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR0824863.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="ACHURQ_003145"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="ACHURQ_003145"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR0824864.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="ACHURQ_003146"
     CDS             complement(5698..7014)
                     /locus_tag="ACHURQ_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR0824865.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="ACHURQ_003147"
     CDS             complement(7004..8761)
                     /locus_tag="ACHURQ_003147"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR0824866.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..>9645)
                     /locus_tag="ACHURQ_003148"
     CDS             complement(8777..>9645)
                     /locus_tag="ACHURQ_003148"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR0824867.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2628 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacaat cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaccga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtag attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata accaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggtggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcga
//
LOCUS       DAZOZY010000151         9619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZOZY010000151 DAZOZY010000000
VERSION     DAZOZY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.152"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHURQ_003149"
     CDS             complement(<1..163)
                     /locus_tag="ACHURQ_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR0824868.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHURQ_003150"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHURQ_003150"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0824869.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHURQ_003151"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHURQ_003151"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0824870.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHURQ_003152"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHURQ_003152"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0824871.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHURQ_003153"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHURQ_003153"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0824872.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHURQ_003154"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHURQ_003154"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0824873.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHURQ_003155"
     CDS             5058..6191
                     /locus_tag="ACHURQ_003155"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0824874.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHURQ_003156"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHURQ_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0824875.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHURQ_003157"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHURQ_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0824876.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHURQ_003158"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHURQ_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0824877.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHURQ_003159"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHURQ_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0824878.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHURQ_003160"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHURQ_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0824879.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHURQ_003161"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHURQ_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0824880.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2439 a         2469 c         2465 g         2246 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacc
//
LOCUS       DAZOZY010000152         9600 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZOZY010000152 DAZOZY010000000
VERSION     DAZOZY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.153"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            442..1608
                     /gene="sopA"
                     /locus_tag="ACHURQ_003162"
     CDS             442..1608
                     /gene="sopA"
                     /locus_tag="ACHURQ_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFR0824881.1"
                     /translation="MSLKKLLQDCVTRGQEMTQAIAIAQFGDDSPEARRITRRWGITE
                     VADLIGVTPQAIRDAEKSGRLPAPDFEMRGRIERRAGYTIDQINSMRAVFGNPNQRPQ
                     DKNPVALAVMSHKGGVYKTSSAVHQAQWLALQGHRVLLVEGNDPQGTASMYHGYVPDL
                     HIHAEDTLLPFYLGERDNAEYAIKPTCWPGLDIIPSCLALHRIETDLMQYHAQGKLPH
                     PPHLMLRAAIESVWDNYDVIVIDSAPNLGTGTINVVCAADVIVVATPAELFDYASVLQ
                     FFTMLLDLLESVDLGGFEPTVRLLLTKYSLTTGNQSRWMEEQIRNTWGSMVLRQVVRV
                     TDEVGKGQIKMRTVFEQAANQRSTLNAWRNAVSIWDPVCREIFDDLIKPRWENE"
     gene            1608..2573
                     /locus_tag="ACHURQ_003163"
     CDS             1608..2573
                     /locus_tag="ACHURQ_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFR0824882.1"
                     /translation="MKQRSLLKNAPDINRIMSNNHHAPEHQPVSPMVGDLKRQLSSLT
                     GNSITLPVCGRNVNFKLETIPADKVEMATMVWLGNERDQDLLNESSLADLIPSFLTSG
                     QQNPAFARKVSGIVEVADGSRRRKAAILTGCDYRVLIGDLDDEQMHWLSQIGNDYRPI
                     SAYERGKRYLRKLNDFDGNVKALAEAENIDRNIITRCINTAGLPKDILAIFNHPGELS
                     ARAGDSLFKVYKKNMAAMSNAAHHLLAIKKNGEDLEASRIIQILSDSVLVGDEEKTKD
                     EKKYGEGITARYKGNFVTIKIDSRKISKEILSRIELLLEEQSTDN"
     gene            complement(2606..3181)
                     /locus_tag="ACHURQ_003164"
     CDS             complement(2606..3181)
                     /locus_tag="ACHURQ_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0824883.1"
                     /translation="MAFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     NSLLEAWKKYRVLLNRVDTSLSPDIEWPSQP"
     gene            complement(3181..6579)
                     /locus_tag="ACHURQ_003165"
     CDS             complement(3181..6579)
                     /locus_tag="ACHURQ_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0824884.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHAADAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATAAGNSAKAAKT
                     SETNAKSSETAAAQSASAAADSKTAAASSASAASTNAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFN
                     PSVFKDTYQYGYTSSGQNTWGVQGSVDMSTGWLANTSTDGNHSHSLSGTAASAGAHAH
                     TVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(6644..7243)
                     /locus_tag="ACHURQ_003166"
     CDS             complement(6644..7243)
                     /locus_tag="ACHURQ_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR0824885.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKRHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(7310..>9600)
                     /locus_tag="ACHURQ_003167"
     CDS             complement(7310..>9600)
                     /locus_tag="ACHURQ_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0824886.1"
                     /translation="YNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETAT
                     ELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLR
                     HVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNP
                     VSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITA
                     VQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKV
                     VKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAANAWGQQGDP
                     ASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDPTVQFEFWFSEKRIADIRQVETT
                     ARYLGTALYWIAASSNIKPGYDYYFYIRSVNTVGKSAFVEAVGRASDDAEGYLNFYKG
                     LINKTHLGKELLENFELTEDNASRLEEFSKEWKDANDKWNAMWGVKIEQTKDGKHYVA
                     GIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAP
                     TITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIE
                     GDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQS
                     YWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTP
                     TTSISDLLVVVMKKSTAGISIS"
BASE COUNT         2262 a         2462 c         2277 g         2599 t
ORIGIN      
        1 ttatgtttcg ttatgttctt gattggtgtt ttcatttgat gtaatgttct cagtttgcgt
       61 actgttttat ttaacatttg tttttattct ttatttatgt tctttattgt gtttgtgtta
      121 ttacttgttt tttatgttct ttatttgtgt tcttatgttg gtaaggttgg gagaactaat
      181 agagaaaacg tatctcattg atgattctta tcatgtttct ctagttttat atgatttcct
      241 ttcttaagaa aggtcataaa tatcaattag ttatgattat attgttgttc gttttatgca
      301 tttcgggagg ggaaaaaaag atgatttagc tcaaatattc gcctttgcgt ttttcgtttt
      361 gctttgcctt ttttatgcaa atttgctaga aataagcaaa gatttaatta ttaagcaaag
      421 ttacaagctg gagatgacag tatgtctttg aaaaagttat tgcaagattg tgttacgaga
      481 ggtcaagaaa tgacccaagc aattgctatc gctcaattcg gtgatgatag tcctgaagct
      541 cggcgtatca cccgccgatg gggtataact gaagtagcag atttaattgg tgtaactcct
      601 caagcaatta gagatgctga gaagagcggg cgtttacctg cacctgactt tgaaatgaga
      661 ggaagaattg aacgtcgtgc tggttatacc atagatcaaa tcaacagtat gcgagcagtt
      721 tttggcaacc caaaccaacg accacaagat aaaaaccctg ttgcgcttgc cgttatgtca
      781 cacaaaggcg gcgtttataa aacatcgtct gcggtacatc aagctcaatg gttagcatta
      841 caaggtcatc gtgttctttt ggttgaaggt aacgatcctc aaggcacggc ctctatgtat
      901 cacggttatg ttcctgattt acatatccat gccgaggata ctttactgcc attttactta
      961 ggagaacgcg ataacgctga atatgctata aagccaacat gttggcctgg gctagacatt
     1021 atccctagct gcctagcttt acatcgtata gaaacagatc tcatgcaata tcatgctcag
     1081 ggtaaattgc ctcatcctcc acaccttatg ctgcgcgcag ctattgaatc agtttgggat
     1141 aactacgatg ttatcgttat agatagtgct ccgaacctcg gtacaggaac cattaacgtt
     1201 gtttgtgccg ctgacgttat tgttgttgct acacctgcag aattatttga ttacgcttct
     1261 gttctacagt ttttcacaat gcttctcgat ctattggaaa gcgttgacct aggtggattt
     1321 gagccaaccg ttcgcttact tctgactaaa tatagtctca ctacagggaa tcaatcacga
     1381 tggatggaag aacaaataag aaatacatgg ggatcaatgg tattacgtca ggttgtacgc
     1441 gttaccgatg aagtaggtaa aggccagata aaaatgagaa cagtatttga acaagcagca
     1501 aaccaacgtt caacattgaa tgcatggaga aatgctgttt ctatatggga ccctgtttgc
     1561 cgagaaattt ttgatgatct aataaaacct cgctgggaga acgaataatg aaacagcgct
     1621 ctcttctaaa aaatgctcca gatattaatc gcattatgag taataatcat catgcccctg
     1681 aacatcagcc cgtatcacct atggttggtg atttaaaacg tcaactgagt tcattaaccg
     1741 gtaacagcat aactttgcca gtatgcggac gtaatgttaa ctttaagtta gagacaattc
     1801 ccgcagataa agttgaaatg gcaacaatgg tatggctagg taatgaacgc gatcaagatt
     1861 tattaaatga atcctctcta gccgatttaa tcccatcttt tctaacgtct ggacaacaaa
     1921 atccagcttt cgccagaaaa gtatctggaa tcgttgaagt cgctgacggc tcacgtagaa
     1981 gaaaagcagc gatattaaca ggatgtgact atcgcgtctt aattggtgat ctcgacgatg
     2041 aacagatgca ttggttatcc caaattggta acgattatag gcctattagt gcatacgaaa
     2101 gaggaaagag atacttacgc aagctaaatg actttgatgg taacgttaaa gcattagccg
     2161 aggctgaaaa tatagataga aatattatca ctcgctgtat taatacagct ggtctaccaa
     2221 aagatatatt agctatcttc aatcatccag gagaactgtc agctcgtgct ggagattctt
     2281 tgtttaaggt ttacaaaaaa aacatggctg ccatgagcaa tgctgctcat catcttctgg
     2341 caattaaaaa aaacggcgag gatttagaag cttcacggat tatacaaata ttatctgact
     2401 ctgttctggt tggcgacgag gaaaaaacaa aagacgagaa aaaatatggg gaagggatca
     2461 cggctagata taaaggtaat tttgtaacta taaaaattga tagccgcaaa atatctaaag
     2521 aaatactaag cagaattgag ttgttactag aggaacaatc tactgataat taagaactat
     2581 cccctgcatt gcaggggata ttgatttatg gttgacttgg ccactcgata tctggagata
     2641 gtgatgtatc aacacggttc agcaacaccc gatacttctt ccaggcttcc agcaacgagt
     2701 tttcttcctc cgttgcaatt tccagatctg cagcatcctg aagcggcgca atatgctcac
     2761 tggctacctg catcaggttg ttttttgttt cttccgcctc ccggatccgg aacagttttt
     2821 ctgcttccgt atccttcacc caggctgtgc cgttccactt ctgaaactcc ccttccgggg
     2881 ataaccaggt gacattttcc ggtaatgggc caagttcaga aataaataac gcgtcgccgg
     2941 aagccacgtc atagacggtt tttccccgat ggtcttcaac aagatgccac gatgcctcat
     3001 cactgttgaa aacagccacg aagccagccg gaatatctgg cggtgcaata tcggtactgt
     3061 ttgctggcag acctgtatga ggcggaatgt atgcgtcacc ttcaccaata aattcattag
     3121 ttccggccag cagattataa attgttatgg tccgtggttg ttcactcatt ctgaatgcca
     3181 ttatgcaagc ctcacaatat agttaaatgc gatgtttttg acggtgtttt ccgcgttacc
     3241 agcagcgtta acggtgatgg tgtgtccatg tgagccaatc gcaaccgagt gcgtatgagc
     3301 accaataccg acagtatgcg cgtgtgcacc tgcagatgct gctgtgccgg acagtgagtg
     3361 gctatgatta ccatctgtac tggtattagc taaccacccc gtagacatat ctactgagcc
     3421 ttgtacaccc caggtatttt gacctgagct tgtataacca tattgataag tatctttaaa
     3481 aacactgggg ttaaatcgac ggccatctct atggctgtga ttaccagctg cattcgtgct
     3541 gccacttaaa ctatgggtat gcgcaccagt gttattcgtg gatttagtgc cgtaatcaaa
     3601 cgacgatgtg gttttcgtcc ccaaatccgt actggatgcg ctggcgctgt gggtgtgcga
     3661 tttaatgccg tcctgttcct gagacaatac ggcccgacca ctggcaggtt ttcccttaat
     3721 cgtccagcca cgcatatcag ggatcacgcc tgacggataa gcggctgcaa gtttcgggta
     3781 agcagatttg tcaaaagtct gcccctgcat cagggcataa ccagacggaa cggtatctga
     3841 tggccacggg attggtgcgc cgactgggta gctttctggt ggaagatttt tcgaggtata
     3901 aacttctgcc cagtcttcct caaaaccata accgtctctt gaagaacggt agaacagacc
     3961 accatttctg taatgcgcct tcatctgcaa ggtccggcaa cttccgactc cggtatagaa
     4021 gttaaccaga atatagctgt cgccagagcg ggtgacattg taagcgcctg attcggcatt
     4081 ccagggaacg ccaccatccg catcggcata tgtatccgtt gcccttctgg caaaagcagc
     4141 cacatgcgcg gcggttaaag taatatcttt ggaaccatca aactcaacac cagaaaccag
     4201 tcttggcgtt tgcagctttg ttgctgttaa tgcattaccg ttcagacttg cggacagttt
     4261 ggttccaata accagttcgc cggttgcgtt atcaatagca aacggtctta atgtattcca
     4321 gccaccataa acatcacctt gattggtaag cagcaggtaa gttttagcgc catcattacg
     4381 ccataatgca ccatactccc cacctatcat tcgaatctga ttaccaccac gcgctacaat
     4441 ttcgtctgtg gcaaaaagtt ttttgcacga caagttatcg ttaacgatta acgaatgaga
     4501 ctcataaaaa ccacgcccac tcttaaaatc aaggataacg tccgccgcga tacattcagt
     4561 cgctggattt gttgccccaa acttataggt cgtatcatta acaacgagat cagcaccagg
     4621 tgcggatatt gacaggccat cttcgataaa cgcaaaaaca gggaaagcag cgccatcaac
     4681 atagaacaca gagcgcaaat catcgccctt attactcatc attattgagt ggatggctcg
     4741 ttcattgttt tgatattgcc agaacattcc ataagcataa cgccccctgt cagtccagcc
     4801 accaggcata acaaatccgt taaactcgca gttattcatc ggatcgcctg cggttcgcgt
     4861 tgccgtggtg ataatgaccc ttgatgccag ttcgcttact gagccagcag aacgcataac
     4921 aacaacaggg taatattttc cagatgttgc acctgcagga gcgttaaccc gcacataacg
     4981 cataccacgc ttatcagcaa agtctgtttt actgaccgcg ttaatgttgt tcaggaagcg
     5041 tcccttatcg ggtatatcag cgccgttctg gtctttctgc agacgtttct ctgcattgtc
     5101 ataggctgat tttactgcct ttggcgttgc cgccagcgtt tcagacgtac tgttggtcgc
     5161 actgctgagc tgtactatcc cctttttcgt cgtacttgca tcctcaagcg ccacggcgga
     5221 tgcaatatcc tctgcccgtt tagctgctgt ctcggcgcgc gttgccgcgg attccgccgt
     5281 acttttgctc tgagctgccg ccgtcgcact gccagctgcc tctgtcgcct tcgtggatgc
     5341 tgtcgtggcg ctgcccttcg ctgctgacgc ctgtctggtc gcctcatctt ttgaagcaga
     5401 cgcagatgat gccgatgacg ccgccgaact ggctgacgat gcggctgccg tttttgagga
     5461 ttctgcgctg gtttccgacg ctttcgcgtt cgtttcggat gtcttcgctg cggaagctga
     5521 cctcgctgct gccgtggcct gttcagtggc ttcgccagcc ttcgttgtgg ctgttgaagc
     5581 ggatgatgcg gcgctttctg ccgactttcc ggcagcggtg gcactggctg aggcctgccc
     5641 ggcatttgtt gacgcggcgc tggcagatga ggcagccgct gtttttgagt ctgctgctgc
     5701 ggaggcactc tgtgctgctg ccgtctcaga agacttagcg ttcgtctcgg acgtttttgc
     5761 cgccttcgcg gaattgcctg ccgctgttgc cgaggaggct gcactactgg cgctcgaggc
     5821 tgcgctcgtt tctgatgatt ttgccgcctc ttttgaagcc gacgcatctc tggctgaggt
     5881 ggcagcttct gacgctttcg tggtcgcggt ggatgcagaa gtggcggctg attgttgtga
     5941 cgctgaagca ttcgtttctg acgttttcgc cgcaccggca ctggtagccg ccgcgctttt
     6001 tgaggactct gcagcggcag cactttttga tgcttcagtg gcctttgttg atgccgttcc
     6061 tgcgctggaa gacgctgact gagccgacga cgcggcctgt ccggctgacg tgctggctgc
     6121 gcgtgctgag tccgcagcat cagccgcatg ggttgccgcc tcacgggctg atgtgctggc
     6181 atcactggct gacttcttcg cggctgccgt gttctgtgcc accgcggacg cgttacgcgc
     6241 cacctcttcc accatcagtt caaaacggcg cagtgcctcc ggacgggcat catcctccgt
     6301 catggcaccg agaaaatcat tcagcgtacc gggttgggaa tcttcataca cggtgatggt
     6361 cccggcatgt gacggcggga atccttccac caacagaata acgctgtact gaccgtactc
     6421 aacgtccatg ctgtaacgcc cggcttcatc cggattttct gaggccagcg tgttcaccac
     6481 caccgtggtg ctgttacgtt ttgctttcag ctggattgtg cagttctgta ccggttttcc
     6541 tgtgccatct ttcagtacac ctgaaatctt tactgccata ttcaccccac aaaaaagccc
     6601 gcctgaaccg gcgggctgtc ataacactgt gttacctggc taatcagaac ttataaccga
     6661 cacccatgat gaaaccgtca gtgcgccagt cgccactgcc ggagccttca taagcaatat
     6721 caatggccac ggattcggtc gggttaaact gcacgccagc tccccacgcc agagacgtgt
     6781 tgctgtggcg accgtcatca cttccggtca gcacgtcgtg cctttttccc ttgttgtcag
     6841 ttacgcggag ataatccccg gaaaaagtcg acacacggct gtaagccacg cctgccatcg
     6901 catacgcgct gaaccattca ttcacgcgta cagacggccc cgccatcacg ctgaaccagc
     6961 ggttacgcac ggaatcttca tgccagcggg tatcgctgta atgggtgagc tggcgattct
     7021 tgtctcctgc atagctgaac gacgtcacca tccccagtgt gtccgtaaac tcataacggt
     7081 atttcacgtt aatcccgttc agatcatcac tgccgggaac gttcgtccgg gcatgaagat
     7141 accccgcgct cagcgtggac tgatgttcag acgcccatgc aggcgcaccg gatacggcca
     7201 gacaaatggc tgcggacaaa atggcggcat aaagtttacg cataattacc tctcgctttt
     7261 ctgcaataaa aaaggcgtca tttctgacgc ccgttctggg ttataaaatt cagctgatac
     7321 tgatacctgc tgtggatttt ttcatcacca caaccagcag atcgctgata ctggttgttg
     7381 gtgtccagtt attcgctcct gatgaagata cggtgaatgt cagtgtcagc gtcccctgtc
     7441 cggcaggcat atctataact gaggaaaata cgccctgagc atccgtcgtg gactgattaa
     7501 aaatctcctg accattgcgg gtcactctta accggcaggt tgaataccag tatgactgtt
     7561 ggttattact gttgaaattc tcatgcttac caccgcggaa taacactggc ggtatcatga
     7621 cctgccggtc aaatttctga tcatcactga ttcttaccgt gatggtgcca ctggcataac
     7681 tgttcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcaccttca atctggttgg
     7741 ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg agcgtcccgg
     7801 agttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt ccgtccggtg
     7861 tcagggaaaa ggccggagga ttgccgccgc tggtaatggt gggggccgtc aggcgcttca
     7921 ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc
     7981 cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg ctcagtttgc
     8041 cttcctccgt gtcctccatg ctgaggccaa tacccgcgac ataatgtttg ccgtctttgg
     8101 tctgctcaat tttgacgccc cacatggcat tccacttatc gttggcgtcc ttccactctt
     8161 tcgaaaactc ctccagtctg ctggcgttat cctccgtcag ctcaaagttt tccagcagtt
     8221 cctttccgag atgcgtttta ttgatcaacc ctttataaaa attcagataa ccttccgcat
     8281 catcgctcgc ccgaccgacg gcctccacaa atgccgattt accaacggta ttcacgctgc
     8341 ggatataaaa gtaataatca taacccggct taatattgct actggcagct atccagtaca
     8401 gcgccgtgcc aagatagcgg gctgtggttt caacctgcct gatatccgca atccgctttt
     8461 ccgagaacca gaactcaaac tgcaccgtcg ggtcatatac agccagtttc gggaccgctg
     8521 ttatctgaaa ataccctggt atcagttcaa tagtgacagg cgctgccggt gcggcaatcc
     8581 ggaacgatac cgatgcagga tcgccctgct gcccccacgc atttgccgcc cggactgtca
     8641 gcctgtagtt ccccagcgcc agttgcgtga agcggtatgt ggtttccgtc gtccgggccg
     8701 tgctgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc ataaagctca
     8761 cgcccttcac caccttcggc gtgtcccagc gcgccagcac ctgatattcc ccgctgtctg
     8821 cggtgacttc ggcagtcagg tgctgcaccg cgggcggcgt gacgccattc accgtgccgc
     8881 tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ttctttttcg ggtacatgct
     8941 gcacggcggt gatggcatac gtgccgtcgt cgttctcacg gatactcacg cagcggaaca
     9001 ggcgctggcg caacgtcggc aacttcagcc cccacacgct gtattctgca acgccgtcag
     9061 gaacacggct cacttttacc ttcacgccgt cggtgacgga ctggacctcc acgctgaccg
     9121 gattgccact tccgtcaacc aggcttatca gcgtggtacc ggaggatggc agcgtgattt
     9181 cacggtcgag cgtcagcgtc cgggtctggc tgtttaccgc cagcacgcgc ccgccgatgc
     9241 tgataccggc atagtcatca tcgcagattt caatgacatc gcccggtaca tggcgaagcc
     9301 cttcggcacc cacgctgaag tccacggtct gcgtttccag cagttccgtt ttaatcagcc
     9361 acagcccggc gcggtgtgcc tgcccccgac tggtacagcc aaaggcatcc atcttcgtga
     9421 cattacgacc gtaacgggca atggcctgcg tgtcctccac aagctctgtt gccgtttccc
     9481 agccgttatc cgggtcaatc cagttcacct caacggcatt atggcggtcc ttcagggcgc
     9541 tgaaactgta gcggaacggc gcgccatcat ccggcatcac cacattactg cggttatagg
//
LOCUS       DAZOZY010000153         9525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZOZY010000153 DAZOZY010000000
VERSION     DAZOZY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.154"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            375..1352
                     /gene="glaH"
                     /locus_tag="ACHURQ_003168"
     CDS             375..1352
                     /gene="glaH"
                     /locus_tag="ACHURQ_003168"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR0824887.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1372..2640
                     /gene="lhgO"
                     /locus_tag="ACHURQ_003169"
     CDS             1372..2640
                     /gene="lhgO"
                     /locus_tag="ACHURQ_003169"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR0824888.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2663..4111
                     /gene="gabD"
                     /locus_tag="ACHURQ_003170"
     CDS             2663..4111
                     /gene="gabD"
                     /locus_tag="ACHURQ_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0824889.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4125..5405
                     /gene="gabT"
                     /locus_tag="ACHURQ_003171"
     CDS             4125..5405
                     /gene="gabT"
                     /locus_tag="ACHURQ_003171"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR0824890.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5716..7116
                     /gene="gabP"
                     /locus_tag="ACHURQ_003172"
     CDS             5716..7116
                     /gene="gabP"
                     /locus_tag="ACHURQ_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR0824891.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7137..7799
                     /gene="csiR"
                     /locus_tag="ACHURQ_003173"
     CDS             7137..7799
                     /gene="csiR"
                     /locus_tag="ACHURQ_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR0824892.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7800..8249)
                     /gene="kbp"
                     /locus_tag="ACHURQ_003174"
     CDS             complement(7800..8249)
                     /gene="kbp"
                     /locus_tag="ACHURQ_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR0824893.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8333..8491)
                     /locus_tag="ACHURQ_003175"
     CDS             complement(8333..8491)
                     /locus_tag="ACHURQ_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR0824894.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8674..8973
                     /locus_tag="ACHURQ_003176"
     CDS             8674..8973
                     /locus_tag="ACHURQ_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR0824895.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8983..9507
                     /gene="ygaP"
                     /locus_tag="ACHURQ_003177"
     CDS             8983..9507
                     /gene="ygaP"
                     /locus_tag="ACHURQ_003177"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR0824896.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2193 a         2447 c         2618 g         2267 t
ORIGIN      
        1 gggaaaacag gagaaccgcc gaactatctc tatttataat ttaatgttat atctgccccg
       61 ataaaacggg gcagataata tgtttagttt actaacggtc attttgcagt gaagccattt
      121 actgtttttt atcgaccaga taatctgttc tctaatgtta actcccccta acctgttgct
      181 ttagttattc atttcctgtc tcactttgcc ttaatactct acgttaaatg ttactaattt
      241 gttgcttttg atcacaataa gaaaacaata tgtcgctttt gtgcgcattt ttcagaaatg
      301 tagatatttt tagattatgg ctacgaaatg agcatcgcca tgtcacccta catctcataa
      361 gaggatcgct tctgatgaat gcactgaccg ccgtacaaaa taacgctgtc gattcaggcc
      421 aggactatag cggattcact ctcatcccgt cggcgcaatc cccgcgtctg ctggaactca
      481 ccttcaccga acagacgacc aaacagtttc tcgagcaggt tgccgaatgg cccgtacagg
      541 cgctggagta caaatcgttt ctgcgttttc gggtaggcaa aattcttgac gatctgtgtg
      601 cgaatcagct gcaaccattg ctgttgaaga ccctgctaaa ccgtgctgaa ggtgcgctgt
      661 tgatcaatgc ggtgggtatc gatgatgtgg cgcaggcaga tgagatggtg aagctggcaa
      721 cggcggtagc gcatctgatt ggtcgctcaa atttcgatgc gatgagcggt cagtattacg
      781 cgcgtttcgt ggtgaaaaat gtcgataact cagacagcta tctgcgtcag ccgcaccgcg
      841 tgatggaact gcacaacgac ggcacgtatg tcgaagagat caccgattac gtgctgatga
      901 tgaaaatcga tgagcaaaac atgcagggtg gaaattcgtt gctgctgcat ctcgatgact
      961 gggaacatct ggaccactat ttccgccatc cactggcgcg tcgcccgatg cgctttgccg
     1021 cgccgccgag caaaaacgtc agcaaagatg ttttccatcc agtgttcgac gttgatcaac
     1081 agggtcgccc ggtgatgcgc tatatcgacc agttcgtcca gccaaaagat tttgaagaag
     1141 gcgtgtggtt gagcgagctt tccgatgcca ttgaaaccag caaaggcatt ctttctgtac
     1201 ccgttcccgt tggcaaattc ctgttgataa acaacctgtt ctggctgcac ggtcgcgacc
     1261 gctttactcc gcacccggat ctgcgccgtg aactgatgcg tcagcgtggc tatttcgctt
     1321 acgccactca ccactaccag acgcatcagt aagcgcaaag gaattgagcg gatgtatgat
     1381 tttgtgatta ttggcggcgg catcatcggc atgtcgaccg ccatgcaact gattgatgtt
     1441 tatccggatg cccgcattgc gttgctggaa aaagagtccg gcccggcctg tcaccagacg
     1501 ggccacaaca gcggcgtgat ccatgccggg gtctattaca cgcccggcag cctgaaggcg
     1561 cagttttgcc tggcggggaa ccgcgccacc aaagcctttt gcgatcaaaa cggcattcgc
     1621 tatgacaact gcggcaagat gctggtcgcc acgtctcagc tcgaaatgga acggatgcgc
     1681 gccttatggg aacgcacagc ggcgaacggt atcgagcgcg agtggataaa cgccgtggaa
     1741 ctgcgcgagc gcgaaccgaa tatcaccggg cttggtggca tttttgtgcc gtccagcggc
     1801 attgtcagct accgcgaagt cacagcggca atggcaaaaa tcttccaggc cagaggcggc
     1861 gagattattt ataacgccga agtcagcgcc ctcagtgagc ataaaaacgg cgtggtgata
     1921 cgcacccgtc agggcggtga atatgaagca tcgacgctga ttagctgttc cgggctgatg
     1981 gctgaccggc tggtgaaaat gctcggcctc gaaccgggct ttatcatctg cccgttccgt
     2041 ggtgagtatt tccgccttgc gccggagcat aaccagattg ttaaccacct gatttacccc
     2101 attcccgacc ccgcgatgcc atttttgggc gtgcatctca cccgcatgat cgacggcagc
     2161 gtgaccgtcg ggccaaacgc ggtgctggct ttcaaacgcg aaggctatcg caagcgcgac
     2221 ttctcattta gtgacacgct ggagattttg agctcgtcgg ggattcgccg ggtgctgcaa
     2281 aaccatctac gctcaggact gggcgagatg aaaaactcgc tgtgcaaaag cggctacctg
     2341 cggctggtgc aaaagtattg tccccggctt tcgttaagcg atctccagcc ctggcccgcc
     2401 ggtgtgcggg cgcaggcagt atcgccggac ggcaagctga ttgacgattt tctgtttgtc
     2461 accaccccac gcacgatcca cacctgcaat gcgccctccc cggcagcgac atcagcaatt
     2521 cctattggtg cgcatattgt cagcaaggta caaacgctgt tggcaagcca gagtaacccc
     2581 ggacgcacgc tgcgagcggc acgtagtgtg gatgccttac acgccgcatt taatcaataa
     2641 cctttgaaaa caggacgtaa caatgaaact taacgacagt aacttattcc gccagcaggc
     2701 gttgattaac ggggaatggc tggacgccaa caatggcggg atcatcgacg tcaccaatcc
     2761 ggcgaacggc gacaagctgg gtagcgtgcc gaaaatgggc gctgatgaaa cccgcgccgc
     2821 tatcgacgcc gccaaccgcg ccctgcccgc ctggcgtgcg ctcaccgcca aagaacgcgc
     2881 caacattctg cgcaactggt tcaatttgat gatggagcat caggacgatt tagcgcgcct
     2941 gatgaccctc gaacagggca aaccactggc cgaagcgaaa ggcgaaatca gctacgctgc
     3001 ctcttttatt gagtggtttg ccgaagaagg caaacgtatt tatggcgata ccattcccgg
     3061 tcatcaggcc gataagcgcc tgattgttat caagcagccg attggcgtta ccgcagctat
     3121 cacgccgtgg aacttcccgg cggcgatgat tacccgtaaa gccggtccgg cgctggcggc
     3181 aggctgcacc atggtgctga agcccgccag tcagacaccg ttctctgcgc tggcgctggc
     3241 ggagctggcg attcgcgcgg gcattccggc tggcgtgttt aacgtagtca ccggttcggc
     3301 aggcgcggtc ggtaatgaac tgaccagcaa cccgctggta cgcaaactgt cgtttaccgg
     3361 ttcgaccgaa attggccgcc agttaatgga acagtgcgcg aaagacatca aaaaagtgtc
     3421 gctggagctg ggcggcaacg cgccgtttat cgtctttgac gatgccgatc tcgataaagc
     3481 cgtggaaggc gcgctggcct cgaaattccg caacgccggg caaacctgcg tctgcgccaa
     3541 ccgtttatac gtgcaggacg gcgtgtatga ccgctttgcc gaaaaattgc agcaggcggt
     3601 gagcaaactg cacatcggcg acgggctgga taaaggcgtc accatcgggc cgctgatcga
     3661 tgaaaaagcg gtagcaaaag tggaagagca tattgccgat gcgctggaga aaggtgcgcg
     3721 tgtggtttgc ggcggtaaag cggatgaacg cggcggcaac ttcttccagc cgaccattct
     3781 ggtggacgtg ccggccaacg ccaaagtgtc gaaagaagag acgttcggcc ccctcgcccc
     3841 gctgttccgc tttaaagatg aagccgatgt gattgcgcaa gccaatgaca ccgagtttgg
     3901 ccttgccgcc tatttctacg cccgtgattt aagccgcgtc ttccgcgtgg gcgaagcgct
     3961 ggagtacggc atcgtcggca tcaataccgg cattatttcc aatgaagtgg ccccgttcgg
     4021 cggcatcaaa gcctcgggtc tgggtcgtga aggttcgaag tatggcatcg aagattactt
     4081 agaaatcaaa tatatgtgca tcggtcttta actggagaat gcgaatgaac agcaataaag
     4141 agttaatgca gcgccgcagt caggcgattc cccgtggcgt tgggcaaatt cacccgattt
     4201 tcgctgaccg cgcggaaaac tgccgggtgt gggacgttga aggccgtgag tatcttgatt
     4261 tcgcgggcgg gattgcggtg ctcaataccg ggcacctgca tccgaaggtg gtggccgcag
     4321 tggaagcgca gttgaaaaaa ctgtcgcaca cctgcttcca ggtgctggct tacgagccgt
     4381 atctggagct gtgcgagatt atgaatcaga aggtgccggg cgatttcgcc aagaaaacgc
     4441 tgctggttac gaccggttcc gaagcggtgg aaaacgcggt aaaaatcgcc cgcgccgcca
     4501 ccaaacgtag cggcaccatc gcttttagcg gcgcgtatca cgggcgcacg cattacacgc
     4561 tggcgctgac cggcaaggtg aatccgtact ctgcgggcat ggggctgatg ccgggtcatg
     4621 tttatcgcgc gctttatcct tgcccgctgc acggcataag cgaggatgac gctatcgcca
     4681 gcatccaccg gatcttcaaa aatgatgccg cgccggaaga tatcgccgcc atcgtgattg
     4741 agccggttca gggcgaaggc ggtttctacg ccgcgtcgcc agcctttatg cagcgtttac
     4801 gcgctctgtg tgacgagcac gggatcatgc tgattgccga tgaagtgcag agcggcgcgg
     4861 ggcgtaccgg cacgctgttt gcgatggagc agatgggcgt ggcgccggat cttaccacct
     4921 ttgcgaaatc gatcgcaggc ggtttcccgc tggcgggcgt caccgggcgc gcggaagtga
     4981 tggacgctgt tgcaccgggc ggtctgggcg gcacctatgc cggtaacccg attgcctgcg
     5041 tggctgcgct ggaagtgttg aaggtgttcg agcatgaaaa tctgctgcaa aaagccaacg
     5101 atctggggca gaagctaaaa gacggattgc tggcgatagc cgaaaaacac ccggagatcg
     5161 gcgacgtacg cgggctggga gcgatgattg ccatcgagct gtttgaagat ggcgatcaca
     5221 gcaaaccgga cgcaaaactc accgccgaga tcgtggcacg cgcccgcgat aagggcctga
     5281 ttcttctctc ctgcggcccg tattacaacg tgctgcgcat ccttgtaccg ctcaccattg
     5341 aagacgctca gatccgtcag ggtctggaga tcatcagcca gtgttttgct gaggcgaagc
     5401 agtagctatg tccgtttgta agagttaacc gttgctaagg gaatgataac gatgcagtaa
     5461 aaaataataa cgatgaggcg aacgacaaag ttaaaaacga ctgcggacag tcccctcgcc
     5521 cctctggaga gagggttagg gtgaggggta aaacacgcgg ctaaaccctc ccctggccct
     5581 ctccctaagg gagagggaga aaaccctccc gctttcgttt ttaagatttt gttgaccgtc
     5641 tcaattaaac gcgtgacccg gcgcgggaaa tgtcggggcg ctctcccaag tgacacactt
     5701 tcgagaggat tcaggatggg gcaatcatcg caaccacatg agttaggcgg cgggctgaaa
     5761 tcacgccacg tcaccatgtt gtctattgcc ggtgttatcg gcgcaagtct gtttgtcggt
     5821 tccagcgtcg ccatcgccga agcgggcccg gcggtattac tggcctatct gttcgccggg
     5881 ctactggtgg ttatgattat gcggatgttg gcggaaatgg cggttgccac gcccgatacc
     5941 ggttcgtttt ccacctatgc cgataaagcc attggtcgct gggcgggcta taccatcggc
     6001 tggttgtact ggtggttttg ggtactggtt atcccgctgg aagccaatat cgccgctatg
     6061 atcctgcact cgtgggttcc aggcattccc atctggttat tttccctcgt cattaccctt
     6121 gccttaactg gcagtaattt attaagcgtt aaaaactacg gcgaatttga gttctggctg
     6181 gcgctgtgca aagtcatcgc tatcctggcc tttattttcc ttggtgcagt cgcaattagc
     6241 ggtttttacc cttatgccga agtgagcggg atctcaagat tgtgggatag cggcggcttt
     6301 atgcccaacg gtttcggtgc ggtattaagc gcgatgttga tcaccatgtt ctcgtttatg
     6361 ggcgcagaaa ttgtcaccat tgccgccgcg gaatccgaca cgccggaaaa acatattgtc
     6421 cgcgccacca actcggttat ctggcgtatt tctatcttct atttgtgctc tatttttgtc
     6481 gtcgtggcat taataccgtg gaatatgccg gggctgaaag ccgttggatc ttatcgctcg
     6541 gttctggaat tgctcaatat tccccatgcg aaattaatca tggactgtgt gatattactt
     6601 tccgtaacca gttgcctgaa ctcggccctg tataccgcct caaggatgct ctactcctta
     6661 agccgtcgcg gtgatgctcc cgcggtaatg ggtaaaatca accgcagtaa aaccccgtac
     6721 gtggcggtgt tactctccac cggcgcggca ttcttaacgg tggtggtgaa ctattacgcg
     6781 cctgcgaagg tatttaaatt tctgatcgac agctccggcg ctatcgccct gctggtttat
     6841 ttagtcatcg ccgtttcaca gttgcggatg cgcaaaattc tgcgagcgga aggaagcgaa
     6901 attcgcttgc gaatgtggct ttatccctgg ctcacctggc tggtcatcgg ctttattacc
     6961 tttgtgttgg tagtgatgct attccgtccg gcgcaacagt tagaagtgat ctccaccggc
     7021 ttattagcga tagggattat ctgtaccgtg ccaattatgg ctcgctggaa aaagctggta
     7081 ttgtggcaaa aaacacccgt tcataatacg cgctgataat gatcaggagt cacaccatga
     7141 ccattacgtc tctggatggc tatcgctggc tgaagaacga tattattcgc ggtaattttc
     7201 aaccggatga aaaattacgg atgagtttgc tgacatcgcg ttatgcactt ggcgttgggc
     7261 cgttgcggga agcactttct caactggtgg cggaacggct ggtcacggtg gtgaatcaaa
     7321 aagggtatcg ggtggcgtct atgtcagagc aggagctgct cgatattttc gatgcccgcg
     7381 ccaatatgga agcgatgtta gtgagtctgg cgattgcccg cggtggcgat gagtgggagg
     7441 cagacgttct cgcaaaagcg catctgctca gtaagctgga agcctgtgac gccagcgaga
     7501 aaatgcttga tgagtgggat ctgcgtcatc aggcgtttca tacagcaatt gtggcgggct
     7561 gtggttctca ctatttgctg caaatgcgtg aacggttgtt tgatctggcg gcgcgctatc
     7621 gatttatctg gctgcggcga acggtgcttt cggtagaaat gctggaggat aaacacgatc
     7681 agcaccagac cctgactgcg gcggtactgg cgcgagatac cgcgcgcgcc agtgagttaa
     7741 tgcgccagca tttactgacg ccaattccca ttatccagca ggcgatggct ggcaattaac
     7801 tactcttccg gaatacgcaa cacttgcccc ggataaattt tatccgggct ttttagcatc
     7861 ggtttattcg cttcgaagat tttattgtac agattagcgt taccgtagac ctgtttggaa
     7921 atggcactca gagtgtcgcc agacttaacg gtataaaact ggctggcagt ggctggtgtc
     7981 gccgttttca cctgatcatc aacactggca ataccggaaa tattccccac cgcaacaagg
     8041 attttctcct tcgcctcctg actcaggccg tcaccagtga ccgtcgcttt gccgtcggca
     8101 atttgaatgt tcactttatc agcatcaggt ataccggttt tgctcagatg ctcctgcacc
     8161 ttcttcgcct gatcgtcttt atcgtgctga cctgtaaccg cgtcccagag tttttctccg
     8221 gcatctttca caaaattgaa cagacccata gctacctcat tcgttaacgg aaacactcag
     8281 aaagtgtagc agaggaacgc ggcgcgttaa cgccgcgggg gtgagcacag gtttaatcgc
     8341 gggtttgcac ccagaacgcg tgaatcaaac cggggatata cccaagcagt gtcagcagaa
     8401 tattaataat gaacgcccaa ccgaaccctt taccgagcag cacgccgagc ggcggtagaa
     8461 tgatggtgat gacgattctc cagaaaccca tatgtactcc ctataagaaa attactcatt
     8521 gtttaaaaag agattttatc tcttaagcgt agtaatttta gcggagcctg ccagtttcag
     8581 cttacgcttt tactctcatt tacgctgctg aggctggtat atgttgcatt tttagtttag
     8641 agcttactta atttagaaaa tacttaaata aatatgactg aactcgcgca attacaggcc
     8701 agtgccgaac aggcagcggc cttattgaaa gcaatgagcc atcctaaacg attgctgatt
     8761 ctgtgcatgc ttagcggttc ccccggcacc agcgcgggag agctgacgcg cattaccgga
     8821 ctgagtgcct ctgcgacatc acagcatctc gctcgtatgc gggacgaagg gcttatcgac
     8881 agccaacggg atgcccaacg cattctatat tccattaaaa atgaggcggt aaatgccatt
     8941 atcgccaccc tgaaaaatgt ctattgtccg taaggagtca ctatggcttt gacaaccatt
     9001 tcgccgcatg atgcacaaga attaatcgca cgcggcgcaa agttaatcga tattcgtgac
     9061 gctgatgaat atcttcgtga acatattcct gaagcagatc tggctccatt atccgtgctg
     9121 gaacagtcag gtcttccacc taaattacgt cgcgagcaaa ttatattcca ctgccaggca
     9181 ggtaaacgca ccagtaataa cgccgataaa ttagcagcga ttgccgcccc cgcagaaata
     9241 tttttactcg aagatgggat tgatggctgg aaaagagctg gattgccagt agcggtaaat
     9301 aaatctcaac ccttgccgtt aatgcgccag gtgcagatcg ctgcgggtgg tttaatatta
     9361 atcggcgttg tactgggtta taccgtaaat agcggtttct tcttattaag tggctttgtt
     9421 ggtgccgggt tactgtttgc aggaatcagc ggtttttgtg gaatggcaag gttgttagat
     9481 aagatgccgt ggaaccaacg agcttgagaa gcgacgccgg acgcg
//
LOCUS       DAZOZY010000154         9500 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZOZY010000154 DAZOZY010000000
VERSION     DAZOZY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.155"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHURQ_003178"
     CDS             <1..108
                     /locus_tag="ACHURQ_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0824897.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHURQ_003179"
     CDS             complement(149..1231)
                     /locus_tag="ACHURQ_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR0824898.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHURQ_003180"
     CDS             complement(1228..3060)
                     /locus_tag="ACHURQ_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR0824899.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHURQ_003181"
     CDS             complement(3275..3631)
                     /locus_tag="ACHURQ_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824900.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHURQ_003182"
     CDS             complement(3689..4111)
                     /locus_tag="ACHURQ_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0824901.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHURQ_003183"
     CDS             complement(4127..4852)
                     /locus_tag="ACHURQ_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR0824902.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHURQ_003184"
     CDS             complement(4874..5620)
                     /locus_tag="ACHURQ_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0824903.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHURQ_003185"
     CDS             complement(5627..6691)
                     /locus_tag="ACHURQ_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR0824904.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHURQ_003186"
     CDS             complement(6763..7188)
                     /locus_tag="ACHURQ_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0824905.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHURQ_003187"
     CDS             complement(7185..7448)
                     /locus_tag="ACHURQ_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0824906.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHURQ_003188"
     CDS             7560..8060
                     /locus_tag="ACHURQ_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0824907.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHURQ_003189"
     CDS             8080..8274
                     /locus_tag="ACHURQ_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR0824908.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHURQ_003190"
     CDS             8274..8564
                     /locus_tag="ACHURQ_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR0824909.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8843..8995
                     /locus_tag="ACHURQ_003191"
     CDS             8843..8995
                     /locus_tag="ACHURQ_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0824910.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9007..9372
                     /locus_tag="ACHURQ_003192"
     CDS             9007..9372
                     /locus_tag="ACHURQ_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824911.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2442 a         2491 c         2017 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaata atgatgctat tgtttcaggc gtcaacccac cccgccccac agaatgcagg
     8701 gcaatacttc gagttaccag acagtggtca ggggttaagt agccagcccg aggcgtaaga
     8761 acatgacggc agggttcaat ctttaactat gcagcaggtt ttttgttccg ccaccccggc
     8821 gttaagggga aatgaggtca gcatggatac tatcgatctt ggcaacaacg aatctctggt
     8881 gtgcggcgtg ttccctaacc aggatggaac gtttaccgcc atgacgtata ccaaaagcaa
     8941 aacgtttaaa accgaatctg gcgcgcgttg ctggttagcc agaaacactg actgatgagg
     9001 ctgacgatgg aatttaaaga tttaccgtct gatgtacaga aaacagcagc tcatacattg
     9061 cattctgtgc tgcgagaaat cgggaaagat attgcaagcg agccagcaaa agatctggcc
     9121 cggaaaatca agaccgcttt cgttgagctt tataatgttg gcactgactc tgaaactgtc
     9181 gagaccaaga ccgtaagttc accaatattc tcacttgagc caagcgtatt atcaggtgaa
     9241 atatgcaccg agatttccag cgaactgctt ccggtaatac gagaagcaat tcgccgtcgc
     9301 ggtttggatg gaagttatga tcatgacgtt ctgcaagttc tcaggacaat ggtgacttca
     9361 ctggggattt gatccctgcg tcctctaagc ttttgatata gcggtcctcc aagaagcggt
     9421 aaattttgtc gaaatcatct tttccctctg gtttattcag aggattacag gacccgtaag
     9481 tagcggcata catttcaacc
//
LOCUS       DAZOZY010000155         9496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZOZY010000155 DAZOZY010000000
VERSION     DAZOZY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.156"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHURQ_003193"
     CDS             122..952
                     /locus_tag="ACHURQ_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR0824912.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHURQ_003194"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHURQ_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR0824913.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHURQ_003195"
     CDS             complement(2081..3541)
                     /locus_tag="ACHURQ_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR0824914.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHURQ_003196"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHURQ_003196"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR0824915.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHURQ_003197"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHURQ_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR0824916.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHURQ_003198"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHURQ_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0824917.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHURQ_003199"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHURQ_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR0824918.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHURQ_003200"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHURQ_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR0824919.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHURQ_003201"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHURQ_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR0824920.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2259 a         2484 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaaccc gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZOZY010000156         9490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZOZY010000156 DAZOZY010000000
VERSION     DAZOZY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.157"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..424)
                     /locus_tag="ACHURQ_003202"
     CDS             complement(<1..424)
                     /locus_tag="ACHURQ_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0824921.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAG"
     gene            682..1287
                     /locus_tag="ACHURQ_003203"
     CDS             682..1287
                     /locus_tag="ACHURQ_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR0824922.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            1327..2190
                     /locus_tag="ACHURQ_003204"
     CDS             1327..2190
                     /locus_tag="ACHURQ_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0824923.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            2180..3727
                     /gene="fdrA"
                     /locus_tag="ACHURQ_003205"
     CDS             2180..3727
                     /gene="fdrA"
                     /locus_tag="ACHURQ_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR0824924.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            3727..5145
                     /locus_tag="ACHURQ_003206"
     CDS             3727..5145
                     /locus_tag="ACHURQ_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0824925.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            5288..6238
                     /locus_tag="ACHURQ_003207"
     CDS             5288..6238
                     /locus_tag="ACHURQ_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR0824926.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            6248..7630
                     /locus_tag="ACHURQ_003208"
     CDS             6248..7630
                     /locus_tag="ACHURQ_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR0824927.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            7846..7920
                     /locus_tag="ACHURQ_003209"
                     /pseudo
     CDS             7846..7920
                     /locus_tag="ACHURQ_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8007..9056
                     /gene="yahK"
                     /locus_tag="ACHURQ_003210"
     CDS             8007..9056
                     /gene="yahK"
                     /locus_tag="ACHURQ_003210"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR0824928.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            9299..>9490
                     /locus_tag="ACHURQ_003211"
     CDS             9299..>9490
                     /locus_tag="ACHURQ_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824929.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDD"
BASE COUNT         2397 a         2368 c         2617 g         2108 t
ORIGIN      
        1 tacccgccag aaaacccagt aacgtcaccg tccagttcat cccgctggca aaaaacagcg
       61 tcatgccgag aaagccaccg ggaataaagg acagtaacca gaaacgccct tgccagacca
      121 ctaccgccga gaagggtacg gtagtaatta cgctcgccca gaacgcggac agtccactta
      181 cagaggccag ccaaccagca gtcagcgcgg cgaaaaacgc ccacaccatc ccggaataat
      241 taaccgccag acttctgata aaccccgatt taccgccgcc tgcggcataa aaactgcaaa
      301 aggcgacgaa gccaatggtt cctaataatt cccatccttg agagagcccg acatgtgaag
      361 acactaactg ccaaagccct gcacaaatac cgacggtgac acccgttgcc gtgagtccgt
      421 tcatcttgtt cccctctggt tagacatgga cgaaagctac tcttacatgt gcagactata
      481 gagaaataaa acctgattga gtgaaagggt atcgggtcaa agaaacaaat attgcactac
      541 cgcacactgc gaaaagattg ttgaatatta ttcaacaaaa aggcgagata cggcgaatta
      601 aatttaagcg ggttctttta tcagattatc gttgctattc tcaattcatc gaaaacagcc
      661 acaaaaacca ggggaatgtg aatgagtata aaaaatctac ctgccgacta tttattggct
      721 gcacaacagg gtgatatcga taaagtaaaa acctgccttg cgctgggtgt cgatattaat
      781 acctgcgatc gtcaggggaa aacggcaatt acgctggcaa gtttatatca gcaatatgct
      841 tgcgttcagg cattaattga tgccggagcg gatattaata agcaagatca tacctgttta
      901 aatccttttt taattagctg tctgaacgat gatttaacgc tactacgaat tattttaccg
      961 gctaaacccg atcttaattg cgtaacccgt tttggcggtg tcggcctgac gcctgcctgt
     1021 gaaaaaggcc atttaagtat tgtaaaagag cttttggcgc atacggagat taacgttaac
     1081 cagaccaacc atgtaggctg gacgccgcta ctggaagcga ttgtgcttaa tgatggtggt
     1141 attaaacagc aggcgattgt gcagttattg ctggaacacg gtgccagccc gcatctgacc
     1201 gataaatatg gcaaaacgcc actggaactg gcgcgggaac ggggctttga agagattgcg
     1261 cagttactga ttgccgcagg tgcataaacc gggaggctgg ctatcaacac accagaaaaa
     1321 cggtgtgtgt gggcgctaac tgcggatgcg gattttctgg cgcagcgggg gcaaggacag
     1381 gttgaacagg tctttgccag agcggtaaat atcgcactcc cggctcgcca gcagttgctg
     1441 acgctgcttt gtgaagagta cgacaatgcg ccaaacagtt gtcggttggc actcactcac
     1501 tttgatgatc tgttccggca tggtgataag gttcagtttg acgatcaagg tattacggtt
     1561 ggtcaacatc ttcatataga gatgagtcgt tgtcggcgtt ggctgtcccc aaccttgcaa
     1621 atgaccgctg tgaattttca ccttatcgcc tggctacagt ggcacgacat tattcatcag
     1681 cacctggggg aaaatgaaac cctgtttaat tatcgcggcg ataatccgtt ttatcaggcg
     1741 ttaaataaag aattacatat taaacgacgg gcagttattc agacagtaaa cgataagcaa
     1801 aatatcgccg cagcggtcgc cagtatgatt gggttaggga ttggccttac gccatcagcc
     1861 gatgattatt taacaggtct ggcgcttatt ttatttattc ccgggcatcc ggcagaaaaa
     1921 tacaaagagg aattttatcg cggtctgcta cgcggcagaa ataacaccac gttattaagt
     1981 gccataacgc tggaagccgc attacaacaa cgctgccggg aaaatattca tcgctttatt
     2041 cacaacatta tttatggcgt ccctgggaat tcaactcagg caatagaaaa aattaaacat
     2101 attggctcca gttccggctg cgacatgctg tatggcatgg ccgatggttg tgcgctgagc
     2161 caaacctacg gagggaatta tgtcagttaa aatagtcatt aaaccgaata cctattttga
     2221 ttctgtctcg ctgatgtcta tctccacgcg tgcaaataaa ctcgacggcg tcgagcaggc
     2281 atttgtggcg atggcgaccg aaatgaacaa aggtgtgctg aaaaatttag gactgctgac
     2341 gccgacgctg gaacaggcga aaaacggcga cctgatgatt gtcatcaatg gtaaatcggg
     2401 tgcggacaac gagcagttgc tggtggagat tgaagaactg ttcaacacca aagcgcaaag
     2461 cggctcgcac gaggcgcgtt acgccactat tgccagcgcc aaaaagcata tcccggaaag
     2521 taacctggcg gtgatttcgg tcaacggtct gtttgccgct cgcgaagcgc gtcaggcgct
     2581 gcaaaacgat ctcaacgtga tgctgttttc cgataacgtc tcagttgaag atgaactggc
     2641 gctcaagcag ttggcccacg aaaaagggct gctgatgatg gggccggact gcggcacggc
     2701 gattattaac ggcgcggcgc tctgttttgg taacgccgtg cgtcgcggca atatcggtat
     2761 tgttggcgca tccggcaccg gcagccagga gctgagcgtc cgcattcatg aatttggtgg
     2821 cggtgtttca caactgattg gcaccggcgg gcgcgacctg agcgagaaaa tcggcggcct
     2881 gatgatgctc gacgccatcg ggatgctgga aaacgatccg caaactgaaa ttatcgcact
     2941 gatttcaaaa cctcccgcgc ctgcggtggc ccgcaaagtg ctggaacgcg cacgtgcctg
     3001 ccgcaagccg gtggtggtct gcttcctcgg tcgcgttgaa acgccggtgg atgagcaggg
     3061 gctacagttt gcccgcggca gcaaagaggc ggcgctaaaa gcggtgatgc tctccggcgt
     3121 gaaacaggaa aatctcgacc tgcatacgct taaccagccg ttgattgctg atgtgcgtgc
     3181 gcgtctgcaa ccgcagcaga aatacattcg tggcctgttc tgcggcggca cgctgtgcga
     3241 cgaaaccatg ttcgcggtga tggaaaaaca tggcgatgtc tacagcaaca ttcagcccga
     3301 tccggaattc cgcctgcaag atatcaaccg cagcatcaaa cacaccttcc tcgactttgg
     3361 cgatgacgac ttcacgaacg gcaagccgca tccaatgatt gaccccacca accgcatcag
     3421 tcgcttgatc gaagaggcgc gcgatccaga agtggcggtg atcgtgatgg attttgtgct
     3481 cggatttgga tcgcatgaag atccggtcgg ctccaccatc gaggcgatca aagaagcgaa
     3541 agcgatcgcc gctgccgaag gacgcgagtt gatcattctc gcctatgtgc tgggtaccga
     3601 tcttgatacg ccatcgttag aacaacaaag ccagatgctg cttgatgccg gagtgattct
     3661 ggcgagcagc agcaccaata ccggattgct ggcgcgtgaa tttatctgca aaggggagga
     3721 agcctgatga gccagtcact gtttagccaa ccattgaacg ttattaacgt cggcatcgcc
     3781 atgtttagcg atgacctgaa aaagcagcat gtagaagtga ctcaactcga ctggacgccg
     3841 ccggggcagg gcaatatgca ggtggtgcag gcgctggata acattgccga ttcgccgctg
     3901 gcagacaaaa tcgccgccgc taaccagcag gcgctggagc gcattatcca gtcgcatccg
     3961 gtgctgattg gttttgatca ggcgattaac gtggtgccgg gcatgacgcc gaaaaccatt
     4021 cttcacgccg ggccgccgat cacctgggaa aaaatgtgcg gcgcgatgaa aggcgcggtc
     4081 accggagcgc tggtgttcga aggactggcg aaagatctcg acgaggcggc tgaactggcg
     4141 gcctccgggg agatcacctt ctcgccgtgc cacgaacacg actgtgtggg atcgatggcg
     4201 ggcgttacct cggcctcgat gtttatgcac atcgtgaaaa acaaaaccta cggcaacatc
     4261 gcttacacca atatgagcga gcagatggcg aagattttgc gtatgggtgc caacgatcag
     4321 agcgtgatcg accgcctcaa ctggatgcgc gatgtgcagg ggccaatgct gcgcgacgcg
     4381 atgaaaatta tcggtgagat cgatctgcgc ttaatgctgg cgcaggcgct gcatatgggc
     4441 gatgagtgcc ataaccgcaa taacgccggg acgacgctgc tgattcaggc actgacgccg
     4501 gggatcattc aggcaggcta ttccgtcgag caacagcgcg aagtgttcga gtttgtcgcc
     4561 agcagcgact acttctccgg cccgacgtgg atggcgatgt gtaaagcggc gatggatgcg
     4621 gcgcacggca tcgaatacag caccgtggtc accaccatgg cgcgtaacgg cgtcgagttc
     4681 ggcctgcggg tcagcggcct gccggggcaa tggtttaccg gcccggcgca gcaggtgatc
     4741 ggcccgatgt ttgccggtta taagccggaa gattcggggc tggatatcgg cgacagcgcc
     4801 atcaccgaaa cctacggtat tggtggattt gctatggcga ccgcgcctgc aatcgtcgcg
     4861 ctggtgggcg gcacggtgga agaggctatc gatttctccc gtcagatgcg tgaaatcact
     4921 ctcggtgaaa accccaacgt caccattccg ctgctcggtt ttatgggcgt gccgtcggca
     4981 atcgacatca cccgcgtggg tagcagcggc attctgccgg tgatcaacac cgccatcgcc
     5041 cataaagatg cgggcgtcgg catgattggc gcgggcattg tgcatccacc attcgcttgc
     5101 ttcgagaaag ccattctcgg ctggtgcgaa cgttacggcg tctgacttac atgtttatgc
     5161 cggatgcggc gtgaacgcct tatccggcct acggatggcc cgagaattgt aggcctgata
     5221 agcgtagcgc atcaggcagt ctggcgttgg tcataacctc atcaccctct gtagcggaca
     5281 taacaacatg aaagagcttg tggtcgttgc cattggtggc aacagcatta tcaaagataa
     5341 cgccagccag tcgattgagc atcaggcgga ggcggtgaaa gccgtggccg atacggtgct
     5401 ggaaatgctg gcttccgatt acgacattgt gctgacccac ggtaacgggc cgcaggtcgg
     5461 gctggattta cgccgtgcgg agattgccca cgagcgcgaa gggctgccct taacgccgct
     5521 ggcgaactgt gtggcagata cgcagggcgg catcggctat ctgatccaac aggcgctgaa
     5581 taaccgactg gcgcgtcacg gcgagaaaaa agccgtcacc gtggtaactc aggtggaagt
     5641 ggataaaaac gatccaggtt ttgcccatcc caccaagccc attggtgcat tcttcagtga
     5701 aagccagcgt gacaaactac aaaaggcaaa ccctgactgg tgttttgttg aagatgccgg
     5761 acggggctat cgtcgcgtgg tcgcctcgcc ggaaccgaaa cgtattgtcg aagcacccgc
     5821 cattaaggcg ctgatccaac aaggttttgt cgtaattggc gcgggcggcg gtggaattcc
     5881 ggtagtgcgt actgaagcgg gggattacca aagcgtggac gcggttattg acaaagatct
     5941 ctctaccgcg ctgctggccc gtgaaattca cgccgacatt ctggtgatca ccaccgacgt
     6001 cgaaaaagtc tgtatccact ttggtaaacc gcagcagcag gcgctggatc gggtggatat
     6061 tgccaccatg actcgctata tgcaggaagg gcatttttct cccggcagca tgttgccaaa
     6121 aatcatcgcc agcctgacgt tcctggagca gggcggcaaa gaagtgatta tcaccacgcc
     6181 ggaatgcctg cctgcggcgc tgcgcggtga aacgggcacc catattatta aaacgtaagg
     6241 acgtaggatg aaagaaagca atagccgccg tgaatttctg agccagagcg gtaagatggt
     6301 caccgccgcc gcgctgtttg gtacctatgt gccgctcgcc catgcggcgg tagctggcac
     6361 cctaaactgc gaagcgaaca acaccatgaa aatcactgac ccgcattact atctcgataa
     6421 cgtgctgctg gaaaccggtt ttgactacga aaacggtgtg gcggtgcaga cccgcacggc
     6481 gcgccagacc gtggagattc aggacggcaa aattgtcgcc ctgcgcgaga acaagcagca
     6541 tccggacgcc acgctgccgc actatgacgc tggcggtaag ttgatgctac ccaccactcg
     6601 cgacatgcat attcatctcg acaaaacctt ctacggcggg ccgtggcgct cgctcaatcg
     6661 tccggcaggc accactatcc aggacatgat caaactcaag cagaaaatgc tgccggagtt
     6721 acaaccgtat acgcaggagc gggcggaaaa actgatcgat ctattgcagt cgaaaggcac
     6781 caccattgcc cgcagccact gcaatatcga accggtttcc ggcctaaaaa atctgcaaaa
     6841 tttgcaggcg gtgctggcgc ggcgtcaggc gggcttcgag tgtgaaatcg tcgccttccc
     6901 gcagcacggt ttgctgctgt cgaaatcgga acccttaatg cgcgaagcga tgcaggcggg
     6961 tgcgcattac gtcggcgggc tggacccgac cagtgtcgat ggcgcgatgg aaaaatccct
     7021 cgacaccatg tttcagattg cgctggacta cgacaaaggt gtcgatattc acctgcacga
     7081 aaccactccg tcgggcgtgg cagccatcaa ttatatggtt gaaacggtag agaaaacgcc
     7141 gcaactgaaa ggtaagctga ccatcagcca cgcctttgcg ctggctacgc tcaacgaaca
     7201 acaggtcgat gcgctggcga atcggatggc ggcgcagcaa atttctatcg cctcgacggt
     7261 gccgattggc acgctgcata tgccgctcaa acagttgcac gacaaaggcg taaaagtgat
     7321 gactggcact gacagcgtta tcgaccactg gtcgccttat ggtctgggcg acatgctgga
     7381 aaaagccaat ctctacgcgc agctctatat tcgtcctaac gaacagaacc tctcccgttc
     7441 gctgtttcta gccactggcg atgtattgcc gctgaatgaa aaaggcgagc gtgtatggcc
     7501 aaaagcgcag gatgatgcca gctttgtgct ggtggacgcc tcctgttccg ccgaggcggt
     7561 ggcgcgtatc tcgccgagaa ccgcaacgtt ccataaaggg caactggtgt gggggagtgt
     7621 ggcaggttga tgcgcatttg aaacgcctgc aacggtgaat tgtaagagat ttaagcccca
     7681 gggagtgggg cgacctcaat atgatagtgt cggcagaaat ggacattatc tttgaggaat
     7741 atgcccttat tgacgttgtg tagataacta tttgtctacg tgaaaagatc atcagttttg
     7801 ccgattttag tcttttacgg atagcaaata tcacacttat aggcgctcgc cttatgggga
     7861 acgttgctgc tctgtttcat tcaggttcgc ggcatgttgg ggctggatcg ggtggtttaa
     7921 atattggcta tattcaatgg acgcgttttg ccgcgatgac atatcaggcg tcgccaaata
     7981 cacatagcta atcaggagta aacacaatga agatcaaagc tgttggtgca tattccgcta
     8041 aacaaccact tgaaccgatg gatatcacac ggcgtgaacc gggaccgaat gatgtcaaaa
     8101 tcgaaatcgc ttactgtggc gtctgccatt ccgatctcca ccaggtccgt tccgagtggg
     8161 cggggacggt ttacccctgc gtaccgggtc atgaaattgt ggggcgtgtg gtagccgttg
     8221 gtgatcaggt agaaaaacat gcgccgggcg atctggtcgg tgtcggctgc attgtcgaca
     8281 gttgtaaaca ttgcgaagag tgtgaggacg ggctggaaaa ctactgtgat cacatgatcg
     8341 gcacctataa ctcgccgacg ccggacgaac cgggccatac tctgggcggc tactcacaac
     8401 agatcgtcgt tcatgagcga tatgttctgc gtattcgtca cccgcaagag cagctggcgg
     8461 cggtggctcc tttgttgtgt gcagggatca ccacgtactc gccgctacgt cactggcagg
     8521 ccgggccggg taaaaaagtg ggcgtggtcg gcatcggcgg tctgggacat atggggatta
     8581 agctggccca cgcgatgggg gcacatgtgg tggcatttac cacttctgag gcaaaacgcg
     8641 aagcggaaaa agccctgggg gccgatgaag ttgttaactc acgcaatgcc gatgagatgg
     8701 cggctcatct gaagagtttc gatttcattt tgaatacagt agctgcgcca cataatctcg
     8761 acgattttac caccttgctg aagcgtgatg gcaccatgac gctggttggt gcgcctgcga
     8821 caccgcataa atcgccggaa gttttcaacc tgatcatgaa acgccgtgcg atagccgatt
     8881 ctatgattgg cggcattcca gaaactcagg agatgctcga tttttgcgcc gaacatggca
     8941 tcgtggctga tatagagatg attcgggccg atcaaattaa tgaagcctat gagcgaatgc
     9001 tgcgtggtga tgtgaaatat cgttttgtta tcgataatcg cacactaaca gattgaaaaa
     9061 attaataaat acgctgtggt ttaacatatt aacttcgctc tccacttaac tttttagtta
     9121 aggagagcga aataatatca aagtagcagt aaaacctata acgtaaattc aaattgttaa
     9181 attaactccc tccagtacac aatacttctc acgttagtta tgagcgattt ctgatagtgc
     9241 ctggtttaat cagcgcttta ttatctgcga cgtttatttt tatttaagag agatcgcgat
     9301 gatatcatta aaagctccgc acaataattt gatgccatat acgcaacaaa gcatacttaa
     9361 tacggttaaa aacaatcagt taccagagga tatcaaaagc tccctggttt cctgtgtcga
     9421 tatattcaag gttttgatta aacaatatta tgattatcct tatgattgtc gtgatgattt
     9481 agtcgatgac
//
LOCUS       DAZOZY010000157         9402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZOZY010000157 DAZOZY010000000
VERSION     DAZOZY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.158"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            206..1072
                     /gene="folD"
                     /locus_tag="ACHURQ_003212"
     CDS             206..1072
                     /gene="folD"
                     /locus_tag="ACHURQ_003212"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR0824930.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHURQ_003213"
     CDS             1074..1286
                     /gene="ybcJ"
                     /locus_tag="ACHURQ_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR0824931.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1394..1915
                     /locus_tag="ACHURQ_003214"
     CDS             1394..1915
                     /locus_tag="ACHURQ_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0824932.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHURQ_003215"
     CDS             complement(1951..3336)
                     /gene="cysS"
                     /locus_tag="ACHURQ_003215"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR0824933.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHURQ_003216"
     CDS             3510..4004
                     /gene="ppiB"
                     /locus_tag="ACHURQ_003216"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR0824934.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHURQ_003217"
     CDS             4007..4729
                     /gene="lpxH"
                     /locus_tag="ACHURQ_003217"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR0824935.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4847..5356
                     /gene="purE"
                     /locus_tag="ACHURQ_003218"
     CDS             4847..5356
                     /gene="purE"
                     /locus_tag="ACHURQ_003218"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR0824936.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5353..6420
                     /gene="purK"
                     /locus_tag="ACHURQ_003219"
     CDS             5353..6420
                     /gene="purK"
                     /locus_tag="ACHURQ_003219"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR0824937.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6557..7450)
                     /locus_tag="ACHURQ_003220"
     CDS             complement(6557..7450)
                     /locus_tag="ACHURQ_003220"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR0824938.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7447..8100)
                     /locus_tag="ACHURQ_003221"
     CDS             complement(7447..8100)
                     /locus_tag="ACHURQ_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR0824939.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8111..9370)
                     /locus_tag="ACHURQ_003222"
     CDS             complement(8111..9370)
                     /locus_tag="ACHURQ_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR0824940.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2091 a         2667 c         2493 g         2151 t
ORIGIN      
        1 ttagacaagg aaataagtca gttccagatt tacaacgcca tcatggacga aaaatgaagc
       61 tttcaatctc agcgacggtg cgccccacct tcgcaagagg tcgcttcacg cgataaatct
      121 gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc cttcgaccta
      181 cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta aaacgattgc
      241 gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag ccggactgcg
      301 ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa tttatgtcgc
      361 aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg acctcccgga
      421 aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg acaacaccat
      481 cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca aagtgctgga
      541 acgtattcac ccggacaaag acgtagacgg tttccatcct tataacgtcg gtcgcctgtg
      601 ccagcgcgcg ccgcgtctgc gcccctgcac ccctcgcggc atcgtcacgc tgcttgagcg
      661 ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga atatcgtcgg
      721 tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga ctcaccgctt
      781 cactaaaaat ctgcgtcacc acgtagaaaa tgccgatcta ctgatcgttg ccgtcggcaa
      841 gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg atgtcggcat
      901 caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg cggctaaacg
      961 cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg ccacgctgat
     1021 tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca cagggtgagt aacatggcga
     1081 cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa ctggaaggct
     1141 ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg aaagccgacg
     1201 gcgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg agttttgctg
     1261 gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca ggccggttct
     1321 cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc gacattttgc
     1381 cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat gcattggttt
     1441 agggttgagt tcgaaagtca tccctccgcg tctgttattt gccggaatca tcctggcgat
     1501 gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca atgtttttgg
     1561 tcatcgcggg tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt tatgtgtgct
     1621 gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt taaccgtctc
     1681 gttgctatcg cacagcatgc tggattcggt aaccactggc ggtaagggtg ttggctggct
     1741 gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag tcgcgccgtt
     1801 tgcattgtct tgttacacca cgccgtacgg gcatcaggtg attatttctg aattgatgtg
     1861 ggtgtggttg ccggggatgc tactgatggg aatgttgtgg tggcgcagac gataaccgga
     1921 tgcgaaaact cgcatccggc aatagcgcaa ttacttacga cgccaggtgg tcccttgcgg
     1981 gccatcttcc agaacgatcc ccatctcgtt aagacggtca cgcgccgcat ctgccgccgc
     2041 ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa tctcagccac
     2101 ttcgctgtcg tctgcctgcg cgccgctttg caggaacgct tccggttctt gctccagcag
     2161 gcctaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg ctgccatatc
     2221 ttctgctttc agacggttta cttcacgcgc catatcaaac agtacggaat aggcttccgg
     2281 ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt cgccaccggc
     2341 aggagcaacg gttttttctg tgccgcgcag ggcagtgtag agacgctcca gcgccgcacg
     2401 cgcctgcttc aggttctctt cgctgtagtt cagctggctg cgatagtggc ccgacatcag
     2461 gaagtaacgc acggtttcag catcgtagta tttcagcaca tcgcgcacgg taaagaagtt
     2521 acccagcgat ttggacatct tctcgcggtc aaccatcacc attccggagt gcatccagta
     2581 gttaacatac tgaccatcat gggcacaggt ggactgcgcg atttcattct cgtggtgcgg
     2641 gaacatcagg tcagaaccgc cgccgtggat atcaaagtgg ttacccagct gcttgcagtt
     2701 cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag acggccagct
     2761 cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc gtttgtcgtc
     2821 gaccacgtca acgcgcgcgc ctgcctgcag ctggtcgaga tcctgacgcg acagcacacc
     2881 ataagtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgccg cataagcgtg
     2941 acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt gcgtcgcgcg
     3001 cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca tttcggcgat
     3061 catgcgatcc accagcgcca caaagctttc gccattttca ttggcacgtt tgatgatttt
     3121 gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga aacgcagata
     3181 gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac agagatcgta
     3241 aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct taaattcctc
     3301 tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt agacatgtgt
     3361 gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata cattgcgtga
     3421 tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa ctgggttgaa
     3481 gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat cacggcgata
     3541 ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc ctggactact
     3601 gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc tttatgattc
     3661 agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg atcaaaaacg
     3721 aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt actcaggctc
     3781 cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc ctgaacttct
     3841 ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt gaaggcatgg
     3901 acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac caggacgtgc
     3961 caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg cgacactctt
     4021 tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt ttctgcgttt
     4081 tttagcgggg gaagctcgca aggccgacgc gctgtatatt cttggcgatc tgtttgaagc
     4141 gtggattggc gacgacgagc ccaatccact ccatcgccag atggcggcag cgatcaaagc
     4201 ggtgtccgat tctggcgttc cctgttattt cattcatggc aaccgtgatt ttctgctcgg
     4261 caaacgcttt gcccgtgaaa gtggcatgac gttattgccg gaagaaaagg tgctcgaact
     4321 ttatggccgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg acgcgggtta
     4381 tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc tcgccctgcc
     4441 gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag aagccaacag
     4501 cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg cgatggaaaa
     4561 acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac atgaacttat
     4621 cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg aaggttcgat
     4681 ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa aaaaccgcaa
     4741 ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg ctattctctg
     4801 tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt cttcccgcaa
     4861 taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta ccatgcagtt
     4921 cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg tttctgctca
     4981 ccgcaccccc gataaactgt tcagcttcgc cgaaagcgcc gaagagaacg gttatcaggt
     5041 gattattgcg ggcgcaggtg gcgcagcgca cctgccaggc atgattgccg ccaaaacgct
     5101 ggtgccggtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg atagcctcta
     5161 ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg gtaaagctgg
     5221 cgcggcaaac gcggcattac tggcagcaca aattctcgcg actcacgata aagagctaca
     5281 ccagcgtctg aatgactggc gcaaagcaca aactgatgaa gtgctggaaa acccggaccc
     5341 gcgaggtacg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg ccgtatgctg
     5401 cgtcaggcag gcgaaccgtt aggcattgct gtctggccag tcgggctgga cgctgaaccg
     5461 gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg gccggaaacc
     5521 gcattaaccc gcgagctggc gcgccatccg gcctttgtga accgcgatgt gttcccgatt
     5581 attgctgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc gaccgcgccg
     5641 tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgatcgttt aggtgaactg
     5701 gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg tttacgcgcc
     5761 aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga gcagggcatt
     5821 aacttctctg gtgaagtgtc gctggttggc gcgcgcggct ttgatggcag caccgtgttt
     5881 tatacgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt cgcttttccg
     5941 caggccaacg cacagcagca ggcgcaagcc gaagagatgc tgtcggcgat tatgcaggag
     6001 ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg tctgctgatc
     6061 aacgaactgg ctccgcgtgt gcataacagc ggtcactgga cacaaaatgg tgccagcatc
     6121 agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca accagtagtg
     6181 aataatccgt cggtgatgat caatctgatt ggtagcgatg tgaattatga ctggctgaaa
     6241 ttgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg taaagtgggg
     6301 catctgaatt tgaccgacag cgacacatcg cgtctgactg cgacgctgga agccttaatc
     6361 ccgctgctgc cgccagagta tgccagcggc gtgatttggg cgcagagtaa gttcagttaa
     6421 gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt accgctccgg
     6481 tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt gcaccgattg
     6541 ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc agcgtctctt
     6601 caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca taaccactca
     6661 ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct aactcatccg
     6721 gtgtggcatg gcgaatggca cgttgctgcg gcgttcccca gttttcatat accgcatcag
     6781 catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac gcagcggcga
     6841 gatctttatc aatcactgct tcactccctg ccccatcttc cgtcacaggc acacctccgc
     6901 cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct tcgctgtcga
     6961 gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca tcacgtttca
     7021 tctgccagcc gtaagccgct tccagtgcct cttgttcttc tggctgataa accggaccaa
     7081 taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg cgcgtcagca
     7141 ccgtcgtaac aggcggcatc tgcggctgtg cgctcaagct ctgcgccagc atatagccaa
     7201 tcatcccctg gctttccgcc accagcacat ccagcggata cggttctacc tctttccacg
     7261 ccagattctg taacgccagc agccccacct gcggcccgtt gccgtgaaca atcgccagcc
     7321 gataagaacg ggccaggcgt gccagcgcgg gtacagcact ggcgatattg cgatattgat
     7381 tttccgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga gctacaacca
     7441 gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca gcagcgtatc
     7501 tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg tacgtttcgg
     7561 gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt gcgcggcata
     7621 acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg aataggcaaa
     7681 aaactggcgt aactcagcag gcagcggatc gcctgccgcc aacgccagtg gcccgaaaag
     7741 cccggtttcc tccgcgcgtt gcatccatgc agctgcaagc ggttgaggat gcgcaggcgg
     7801 cgtaatacgc agcaaacaat aacgctgtgg ctgtttaacc gtgaaacgcc cgaggcgaat
     7861 cccttgagcc acaacctgtg gtcgttcatt gccgcataat ccaccgcata acgcatcgaa
     7921 ttgcgcgcgg cgaagcaccc atcctccggg gccgaaacca ctaccctgac gatgcagcgt
     7981 taacagttcg ccgctttccg tcatcaggtt aatcgcccgt cgccacactc ccgctaaccg
     8041 ccaggactgg cgataattcg gtgcactacg gctggcaagc agaggatgga tgatcgtcat
     8101 tgcgcgttcc tcaaccaatc cccatgcttt cagccagtgc ttccagcgcc tgttcaaaac
     8161 acgccagcgg tgcccgcaca gtgcctgcgc caatctgccc gatccccgcc tctttatggg
     8221 cgataccggt attgatgagt ggcgtaatgt cggtttctac cacgcgacga atgtccagtc
     8281 ccaggcacgc gccctgaaaa tcccagcctg ggatctgcaa ctgcatattg cgctcaaggt
     8341 aaatttccgc catctcttca gataccgctc tggccgcttc catgccaccc gcaccgacaa
     8401 agcgcgttac gccaggcgct gcgatcattg ccgcacctcc gataccaaag gtttcggtaa
     8461 tcgcgctatc gcccatatcc gggttcgcct gctcctgcga gaagccggtg aaaaacagac
     8521 cttgcggagt gtttacaggc gcagtaaacc agcgttcgcc cagcccgctt acccgaatcc
     8581 cgaacatatt gccgttgcgg gtcattgccg tgacgatgct gcctgcgcgg atcatcgcgc
     8641 cagcatccat cgccgccttg cagtaagcca tcgcgaggtt gaggaagaac tgatcggtca
     8701 cgctgaggaa atccatcact tcggcgatgt gctgtttatc atgatcgagg cgagcaattt
     8761 gtggggccag cgcacgcatt aacagtgcag aggaagcaat attgcgttga tggaactcat
     8821 cgcccatcgt aatgccctgc gccatcatcg ccgtgagatc gataccgcgc tccatgcgcc
     8881 ccagcgccgc gcttaatact ggcattaaca catcgcgcat ccagcgatgg cgagtcagga
     8941 catcttcgcc gtaagcgcca aaacgcatca ctttgccgat accttcgttg aggttgcagt
     9001 acgcccggtt gccgtcggtc acgttctcga ccaccagcat cggcatactg gcagaagtaa
     9061 taccgcccat tggaccgacg gcattcacat ggtgacaagg aatgaagttc acttccccct
     9121 gctccagtat tgccagcgcc tgcgcttcat ctttcgccca gccttcgaac agacatgcgc
     9181 ccacgcacgc ccctttcatg ggtccggtca tctcctgcca gcgcattggc ggcccggcgt
     9241 gaagcagtgt tttgccctcg tttagttcgc tgataagtga agaagccggt tgcacatcca
     9301 gccagtgtgg acgagcgcga cgaatttgtt cgataaccgc agcattggct tgcgccactg
     9361 atgtaaacat gggaacccct tattgcaaac gttctaataa ac
//
LOCUS       DAZOZY010000158         9392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZOZY010000158 DAZOZY010000000
VERSION     DAZOZY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.159"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(270..1013)
                     /locus_tag="ACHURQ_003223"
     CDS             complement(270..1013)
                     /locus_tag="ACHURQ_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0824941.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHURQ_003224"
     CDS             1229..1489
                     /gene="dinJ"
                     /locus_tag="ACHURQ_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR0824942.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHURQ_003225"
     CDS             1492..1770
                     /gene="yafQ"
                     /locus_tag="ACHURQ_003225"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR0824943.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHURQ_003226"
     CDS             1926..2666
                     /gene="dpaA"
                     /locus_tag="ACHURQ_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR0824944.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2637..3404)
                     /locus_tag="ACHURQ_003227"
     CDS             complement(2637..3404)
                     /locus_tag="ACHURQ_003227"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR0824945.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHURQ_003228"
     CDS             complement(3610..4188)
                     /gene="lpcA"
                     /locus_tag="ACHURQ_003228"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR0824946.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4428..6872
                     /gene="fadE"
                     /locus_tag="ACHURQ_003229"
     CDS             4428..6872
                     /gene="fadE"
                     /locus_tag="ACHURQ_003229"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR0824947.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="ACHURQ_003230"
     CDS             complement(6915..7361)
                     /gene="ivy"
                     /locus_tag="ACHURQ_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR0824948.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7542..8312
                     /gene="yafV"
                     /locus_tag="ACHURQ_003231"
     CDS             7542..8312
                     /gene="yafV"
                     /locus_tag="ACHURQ_003231"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR0824949.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(8354..>9392)
                     /locus_tag="ACHURQ_003232"
     CDS             complement(8354..>9392)
                     /locus_tag="ACHURQ_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR0824950.1"
                     /translation="ICAVISGAEGWEDIEDFGETHLDFLKQYGDFENGIPVHDTIARV
                     VSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTM
                     HSLVLGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLF
                     AVKGNQGRLNKAFEEKFPLKELNNPEHDSYAISEKSHGREEIRLHIVCDVPDELIDFT
                     FEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKL
                     HWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNY
                     LASVLAESGLS"
BASE COUNT         2282 a         2345 c         2394 g         2371 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataaac cattattata aaattaataa atatatttca gtttattatg taaaaacatc
     1141 cgtttattaa attactcatg gattgtcgct gttgctcatt tgagctacaa ttcaagctga
     1201 ataaatatac agcacaggag atcccccaat ggctgctaac gcgtttgttc gcgcccgaat
     1261 cgatgaagat ctgaagaatc aggcggcgga cgtactggcc gggatggggc tgaccatctc
     1321 tgacctggtt cgcataaccc tcacaaaggt cgcgcgtgaa aaggcattgc cgtttgattt
     1381 acgcgagcct aatcaattaa ccattcaatc aatcaaaaac agcgaagctg gcgttgatgt
     1441 tcataaggcc aaagacgctg atgatttatt tgataaatta ggaatttaaa tatgattcaa
     1501 agggatattg aatactcggg acaattttca aaggatgtaa aacttgcaca aaagcgtcat
     1561 aaggatatga ataaattgaa atatcttatg acgcttctta tcaataatgc tttaccgctt
     1621 ccagctgttt ataaagacca cccgctgcaa ggttcatgga aaggttatcg cgatgctcat
     1681 gtcgaaccgg actggatcct gatttacaaa cttaccgata aacttttacg atttgagaga
     1741 actggaactc acgcggcgct ctttgggtaa agcacgctgc cgatgaaatt tgtaaaccct
     1801 gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca aatccagact
     1861 attccgattt gggatctatt ttgccgtcct ggcgtgtgta accgttttat caaggaataa
     1921 gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc gtttcgtttg
     1981 ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat aagcagcagt
     2041 tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc gatctctacg
     2101 tcaaaatggg cgagcaatat cagctgctcg acagttataa aatctgtaaa tattccggcg
     2161 gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt tatagcgtcc
     2221 agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt ggtttcccca
     2281 atgcctatga ccgtgcgcat ggttatgaag ggaaatacct gatgatccac ggcgattgtg
     2341 tttccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc cagttcgtta
     2401 ctggtgcgct ggtgtttggt cagccgagcg tgcaagtgag tatttacccg ttccgcatga
     2461 ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg gagcaactga
     2521 agccgggtta cgactacttt gagcaaaccc gtaagccacc gaccgtttct gtggtcaatg
     2581 gtcgttacgt ggtcagcaag ccgttaagcc acgaagttgt gcagccacaa ctggcatcaa
     2641 actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatctt ttgccaggtt
     2701 tcattgcccg tcagcggctg tgttgcaatc accgtgacca catcattcgg tgtggtctgc
     2761 gagctgaagt cgatttccac atcctgatcc agcagcgttg ccacgccaaa cggcgcgcgg
     2821 cgggtgatcc agtgtaaatt agtcgagcaa tacgccatca catagcgccc gtcagaaagc
     2881 agcatgttga aaacgccctt ctgccgcagt tcatccgcca gtgaggcgat atatttaaac
     2941 actgccgcca tgttgcccgg tgtgcgcggg taacgctgcg ttaatttatg caggagccag
     3001 cagaaggctt tttcgctatc ggtttcaccg accgggcgga agttgccggt ttccagtgat
     3061 ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca taactcacgg
     3121 gtaaacgggt gagtattttc cagcgccacc tcgccccgat tggcctggcg aatatgagcc
     3181 accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg ggaattaaag
     3241 ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt aatgccccag
     3301 ccatctttat gtggcccggt tccaccacca cgctgcacca gaccggtgaa actaaagcag
     3361 atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa cctccacaac
     3421 agcgggagcg catggccccc ggcgaagatt gatttaagaa ttttcaggcc ggatgcggcg
     3481 taaacgtctt attcggccta cggttcgcgt acagatttgt aggcatgata agacgcatca
     3541 gcgtcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct tatccggcct
     3601 acgccagact tacttaacca tctctttttc aattaactgg atcaggatat ggatcacttt
     3661 aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa tttcgatatc
     3721 cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt tcatcccctt
     3781 ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg tggagatccc
     3841 cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa tatcattgaa
     3901 accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa tcgccgggta
     3961 gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca tagcgtcgca
     4021 atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc tgtctgctaa
     4081 caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa agttagccag
     4141 cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga ggatatcctt
     4201 cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc gagaagtacg
     4261 ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag caacaatgtg
     4321 agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc actacaacca
     4381 tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg atgattttga
     4441 gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg agcttattta
     4501 tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt ctgtggtcag
     4561 cgtgggtact ggtgcctctg gccattatcc tcgtgccatt taactttgcg cctatgcgta
     4621 agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg ccgatgtcgc
     4681 gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac ttgttccagg
     4741 gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc gccgaagagc
     4801 aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat ttccagatta
     4861 ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag catcgcttct
     4921 tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat gcccagtctc
     4981 gcgtgctgca aaaactctcc ggcgtgagcg ggatcctggc gattaccgtc ggcgtgccga
     5041 actcattagg cccgggcgag ctgctgcaac attacggcac tgacgagcag aaagatcact
     5101 atctgccgcg tctggcgcgt ggtcaggaga tcccttgctt tgcactgaca agcccggaag
     5161 cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc gaatggcagg
     5221 gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctatattacg ctggcaccga
     5281 ttgcgactgt gcttgggctg gcgtttaaac tctccgaccc agaaaagctg ctgggtggcg
     5341 cagaagattt aggtattacc tgtgcgctga tcccgaccac cacgccgggc gtggaaattg
     5401 gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc ggtaaagatg
     5461 tcttcgtacc gatcgattac atcatcggtg ggccgaaaat ggccgggcaa ggctggcgga
     5521 tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac tcaaccggcg
     5581 gcgtgaaatc ggtagcgctg gcaacgggcg cgtatgctca cattcgccgt cagttcaaaa
     5641 tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc ggtaatgcct
     5701 acgtgatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc gaaaaacctg
     5761 ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag tcgattattg
     5821 atgcgatgga tatcactggt ggcaaaggca ttatgctcgg gcaaagcaac ttcctggcgc
     5881 gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt ctgacccgca
     5941 gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg gaagagatgg
     6001 aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa catatcggtc
     6061 acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt ttaaccagca
     6121 gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc ctgagcgcca
     6181 acctcgccct gctttctgat gtctcgatgg cagtgctgga cggcagcctg aaacgtcgcg
     6241 agcgcatctc ggcccgtctg ggggatattt taagccagct ctacctcgcc tctgccgtgc
     6301 tgaagcgtta tgacgacgaa ggccgtaatg aagccgacct gccgctggtg cactggggcg
     6361 tacaagatgc gctgtatcag gctgaacagg cgatggatga tttactgcaa aacttcccga
     6421 accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt cattatctgg
     6481 caccttctga caagctggat cataaagtgg cgaagatttt acaagtgccg aacgccaccc
     6541 gttcccgcat tggtcgcggt cagtacctga cgccgagcga gcataatccg gttggcttgc
     6601 tggaagaggc gctggtggat gtgattgccg ccgacccaat tcatcagcgg atctgtaaag
     6661 agctgggtaa aaacctgccg tttacccgtc tggatgaact ggcgcacaac gcgctggcga
     6721 aggggctgat tgataaagat gaagccgcta ttctggtgaa agctgaagaa agccgtctgc
     6781 gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg gtaaagttgc
     6841 cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa aggctccgtt
     6901 tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag gctgccggtc
     6961 aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac attcagccag
     7021 gtgagtttct cttgcgacgt tttctcatca atagtcgaga acagccccgt catctgatta
     7081 gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg tttgcacgcg
     7141 ctcatcacct gatacgtatc atctcccaac gttacggttt gtgcgggagt ataagtaccg
     7201 cctttcatca cccaggcagg cagcttatgc ccttgtacca tctgattaaa tgcagctttg
     7261 gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat tgcactggtg
     7321 gcgatgacca gagcggcgac tgtcgttatt gtcttaaaca tcattcctcc cgagcttatc
     7381 ctgcccatgt tattaacctt ctaaagcctg gcgataacac gataatttgc aaatcagata
     7441 tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc ctgataaggt
     7501 gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctggt ttgaagatta
     7561 cgcttttgca gcaaccactg gtgtggatgg atggccctgc caacctgcgt cattttgatc
     7621 gtcaactgga aggtattacc gggcgcgatg tgatcgttct accgaagatg tttaccagcg
     7681 gctttgccat ggaagcggca gcttcgtcgc tagcacaaga tgacgtagtg gactggatga
     7741 cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta caaacggagt
     7801 ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat ttttatgata
     7861 agcgtcatct gttccgcatg gcagatgagc atctacatta taaagcgggc aatgcgcgag
     7921 tgattgtgga atggcgcggc tggcgtattt tgccgttggt gtgctacgac ttacgttttc
     7981 cggtgtggtc gcgcaatctc aacgattatg acctcgccat ctacgttgcc aactggcctg
     8041 ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag aatcaggcgt
     8101 atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat cgcggtgaca
     8161 gccgggtgat taatccacaa ggcgagatta tcgctactgc cgacgcgcat caggcaacgc
     8221 gcattgatgc ggagctgtcg atgatggcat tgcgggaata tagagaaaag tttccggcat
     8281 ggcaggatgc ggatgagttt aggttgcggt gagtgggggt tgtgtttaag gctgggggag
     8341 agtcggggta agactacgaa agcccgctct ccgcaaggac tgacgccaga tagtttctgt
     8401 ccatagctgc ttttcgcatc ttacgtctta accctgcctt gaatacctta tcattcgtca
     8461 aaatattaat agcgatgtgc cgtatccctg aaaataattc tgctgcattt cctcttctta
     8521 ttttgcagtc gtcttcattc attaccacgt ccagacgcca gtgcagctta ttctccacgt
     8581 gccagtggtt tcggattgct gtggcgaact tctctgcggt taaatcagca gaactgatat
     8641 aatatctgac cgtcatttct ggctctttct tttgttctgc tattatggac cgaaaggaga
     8701 ctgccacgca taatttcttc agccctttcc attcaaacgt gaaatcaata agttcatcag
     8761 ggacatcgca aacaatatga agacggattt cttctctgcc gtgactcttt tcactgattg
     8821 cgtaactgtc atgctctgga ttatttaatt ctttcagcgg aaatttttcc tcaaaggctt
     8881 tattaagccg cccctggttt ccttttaccg cgaataaata atcaccgccc tgtttttgta
     8941 tcttctctgc aatatctttc tggcatccca tcgcatcagt tgtgatgatt tttcctttaa
     9001 tatccagcat gttaagaagt tcaggaatgg ctgtaatctc attggatttc tcatccgtct
     9061 taatctgccc gagaaccaga ctgtgcattg ttgagaacgc actaatgaca tgaatcgctc
     9121 ccttgcggcg actcttgtca taagagtgcc ggagcgtttt tccatcaatt gcaatgacgt
     9181 ctttatcatc tgaagaatgg cagtcacgca tccagttaat aaagcactcg tgaaattttg
     9241 caggactgat acaggataca actctggcaa tagtatcgtg aacaggaata ccattttcaa
     9301 aatcaccata ttgcttcaaa aaatcgagat gtgtttcacc aaaatcctct atatcttccc
     9361 aaccctctgc accagaaatg acggcacaaa ta
//
LOCUS       DAZOZY010000159         9073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZOZY010000159 DAZOZY010000000
VERSION     DAZOZY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.160"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(507..1274)
                     /gene="fecE"
                     /locus_tag="ACHURQ_003233"
     CDS             complement(507..1274)
                     /gene="fecE"
                     /locus_tag="ACHURQ_003233"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR0824951.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="ACHURQ_003234"
     CDS             complement(1275..2231)
                     /gene="fecD"
                     /locus_tag="ACHURQ_003234"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR0824952.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="ACHURQ_003235"
     CDS             complement(2228..3226)
                     /gene="fecC"
                     /locus_tag="ACHURQ_003235"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR0824953.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="ACHURQ_003236"
     CDS             complement(3223..4125)
                     /gene="fecB"
                     /locus_tag="ACHURQ_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR0824954.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="ACHURQ_003237"
     CDS             complement(4170..6494)
                     /gene="fecA"
                     /locus_tag="ACHURQ_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR0824955.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="ACHURQ_003238"
     CDS             complement(6581..7534)
                     /gene="fecR"
                     /locus_tag="ACHURQ_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR0824956.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="ACHURQ_003239"
     CDS             complement(7531..8052)
                     /gene="fecI"
                     /locus_tag="ACHURQ_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR0824957.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8189..8257
                     /gene="ykiE"
                     /locus_tag="ACHURQ_003240"
     CDS             8189..8257
                     /gene="ykiE"
                     /locus_tag="ACHURQ_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR0824958.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8344..9041
                     /locus_tag="ACHURQ_003241"
                     /pseudo
     CDS             8344..9041
                     /locus_tag="ACHURQ_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1988 a         2510 c         2585 g         1990 t
ORIGIN      
        1 acacttttta gaaaaatcga tggtcagaaa ctggattagc aattccgttc cagggttgct
       61 tttgatttac gttggcgtct gatcattgat ttatcctcaa aagcccaacc tcattggtaa
      121 tgaaccagct ccgtgaatgt ccgctctggc acagagcgaa attttttgat ctcccccccc
      181 tgaaatctaa acttagtcat gtcacgtttt tgggtttcta aaattttaac ttcgcgtttt
      241 tcgttgccgt aagggttata cagaaatgtc cgttaagcag agttcaaaat tgattgccgt
      301 gatcacgact ggtttgaaag ccgcgcccaa gcctgtacag ctctggtttg cgttgattat
      361 gaacctgtca gcctaaagca agcggatgga cgatgagtat tggtaatctt tcagagtccg
      421 gaaaagttca gccccagtct gaacaggctt gctggcgcca gtccagtttc attcagtcgt
      481 ggtttggttc ttacggcctg tgcaatctac ctcattaggc acatcggcct gccagatacc
      541 ggctcggggt gtatttccgc ttccacgctg aatactgttc tcagcaatcc tggggtcatc
      601 acctcttctg gtgtgccttg cgccataaca tgtccgtttg ccattaccac cagttgatcg
      661 cagtaccggc tagcctgatt aaggtcgtgc agcacagcga ccaccgtttt cccctgagtc
      721 cggagttcgc ccatcaaccg catcaggtcc acctggtgat tgatatcaag ataggtggtt
      781 ggctcatcaa gtaatacaac gggcgtattc tgggccagga ccattgccag aaatgcgcgc
      841 tggcgctgac cgccggaaag ctcggttaac cgacgaacgg caagatgatt gatccgggtc
      901 tggttcatgg cgacattaac tcgtgcattg tcttcagcgg agagacgccc ccagagtgac
      961 agccagggat tacgaccata cgaaaccagc tcctggactg tgatcccctc tggcgttaaa
     1021 tggtgctgag gcagcagcga aagcctgcgg gccaactggc gcgatgagag catatttatg
     1081 ggattatcgc cgagaaatac ggtgccagac tgcggcatta aaagccgcga aaaacagttt
     1141 aacagcgtcg atttcccgca accgttagga ccgatcaggg cggtgatctt ccccgttggc
     1201 agtgagagtg aaacgtcgtt aagtaccttg tctgtcccgt aattgaccgt cagattttca
     1261 gttcgtaaag tcatttatcg cattctcaca agcaaccaga caaaccacgg cgcaccgata
     1321 atggcggtca gcacgccaac cgggagctcc agtgggggat gaataattct cgccagcaga
     1381 tcggcaacca ccaacagcaa cgcacctgtc agggccgaaa caggcagcag tctgcggtga
     1441 cgtccaccgg tgatgctacg catcatatgc ggcaccacga gaccaataaa gctaatcggg
     1501 ccgcaggcgg ccacgccggt agatgtcatg gcgacagcta gtaacaaagc ccagaatcgg
     1561 gtatagggca ccgacacacc gagcgtggtg gcgcgcgcat cgccgagtgc aaggaggtcg
     1621 agatcgcggc aaaaactcag gctcagcggc agaaataaaa tcatcagcgg gatggcaatc
     1681 ttcacaaagc tccagtcacg gccccataag ctgccggtca gccacagcag ggcgttgttc
     1741 acatcctgcg ggcgcgagag catcagataa tccgtcaggc tggcccagca tgcagaaagc
     1801 gccacgccgg tgagtgccag cttcatcggc tggtgggtct ttgccagcat cttcagcaat
     1861 atcaaccccg ccatgccgcc cgcaaaggcc agcagcggca gcaccatcac gggcagtgac
     1921 ggcataagaa gtagagcccc cacagaggcc aggctggcgg catggttaac gccgagaata
     1981 tccggtgatg ccagagggtt gcgcacaatc ccctgtatca gcacgcccgc cacggcgagg
     2041 gctgcaccga caaacagtgc cagcagcaag cgcggcagtc ggtactccat caatacataa
     2101 taatgctcgc gtccggcctg ccagtcggtc agcagcgcgc gccacggcac ggggatcact
     2161 cccatatgga gtgataacag cgcacagccc gccagggcaa gggtgatgaa aataaccagc
     2221 gcaattttca tcctcgcctc ctcacaagcc agacaaagca agggctgcca atcagcgcca
     2281 gcactgcgcc tgcgggcaga tctccgggga aggccagcgc gcgtgcgagt acatctgcca
     2341 gcagcatcag cgtggccccc agcagcatgc tcactggcag tacgttgcgc tgatcgaagc
     2401 ctgcccagaa gcgcgccaga tgtggcacca gcagaccgat aaacgccacc ggacctgcca
     2461 cgctgacgca cgcaccaacc agaagcagca ctaacatatt gatgaccaaa cgtagcctcg
     2521 tcaggttcac tcccagcgta tgggcggtgc tgtcgctgag gttgagcagg ttcagttgat
     2581 tcgccagcag caacacgaca gggactgcag tgaccaccac cggcaagagc tgccagacat
     2641 cctgccagcg ggcgtgggac actccgccag ccagccagta aaagatgccg taagcatgat
     2701 cttcggccag cagcagggtg atgcgggtaa ggcccataca aaaggccgac agcgcgatac
     2761 ccgcgaggat cagtttgttt ctgtcatggg tatgacgaaa tccgcctcct gcggtcatga
     2821 ccagcagcca gctcacgccg cccccgcatg ccgcgatgaa cgacagagaa tagcctgcaa
     2881 tcggcgtcgg actcagcgcg ctggtaagcg ccatagccag cgccgcgccg ctgttaatgc
     2941 cgagcagtga aggagaggcc attgggttgt gggtcagggt ttgcagcagc gtgcccgcga
     3001 gcgccaggct tgcgccgatc agaacggcga ccaggcttcg tggcaaacga aggttttgca
     3061 ccagcgcttc tggtagcgtt ggcgtgtgtc caggcagcag ggcgcgggtt gcatctgctc
     3121 cggaaacagg aatggccgag tagcaaaaca gactcagcca gaaaataata ataagtgctg
     3181 caacgggaag cccccacagc agcaccgggt gttttatcgc ggtcatttca caacggtaag
     3241 cggctgatgg tggaagattt ttaccgtgtc agcggcaata cgctctgcag caaaaatacc
     3301 gcgcatccgc gcccaggtgt tactgtcgac cgaagcaacc tgctgcttct gcgcggcggt
     3361 taacatctgc cagagcggat cttgttgcca gcgtttaaca atgctctctt cgcgatagtg
     3421 ggcaaccagc agccaggcag gattgaccgc cagcagttgc tccaggccga tggacggcat
     3481 ggacgcaccc gccatcgcag cgggaacgtt cagccccaga gaggccagca cgctgccggt
     3541 ccaggtctcc tgagtatgca ggttgaattg ctgttcgcgt gatgtgccaa aggccacgcg
     3601 tgtccctttg ggaagctggc tggcccactg cgccatcctc tctttatgtt gttccagacg
     3661 tgcctgcatc tctcgctttt tacccaccat ttcgccgatg atagccgcag attgcaaatt
     3721 ttcagcgtag gtttcgttgc gggacttaag cagcagtacc gacgcgattt gctgcaaggc
     3781 gatgtaaacc cccgcatggc gactgctgtc ggcaatgatc aggtctggtt tcagagcggc
     3841 aatggcttcc aggctcggct gcgcgcgcgt tccgacggac tgccacggtt tcaggtgcgc
     3901 acgcacttcg ggcaggatgc gttttgcatc gttatcgtcg gcaataccga tcgggctgac
     3961 gtccacggcg gccagcgcat cggcgaacga gagttccagc accacaatcc gttgtggcgt
     4021 tttttcgagt gtaaacgtgc cgtgttcgtc ctgaaccgtg gcggcaaagg cgtggctgat
     4081 caccagcagc aggcctgcaa aaagaaaacg gataaatgcc aacataatca cattccagct
     4141 aaaagcccgg caagccgggc gttaacacat cagaacttca acgccccctg catatacagc
     4201 gtgcgcggct ggcctgcata gatgcctttg ttgttgtcgt cataagagcg gatgaagtag
     4261 tcctggtcga agatgttttt cacaccgaat gccaggttca gatctgccat ctgcgggcca
     4321 aagtcataca ccacgcgtgc gccccagagc atgaagccgg gaatgcggcc ggtactgccg
     4381 tcggcgctct ctttcaccgt attggcgtta tccgcaaact ggctggactg gaaatcgctg
     4441 ttcagattga acatccagtt tcctggcttg tagtccacgc ccagcgtgcc tttatgtttc
     4501 ggggagaatg gtaccagatt gccgtaggtg tcgcctttct cgcggatttc cgcgttcaca
     4561 tacgcatagc tggcgtagat ggaaacgtta tcaagcgttg gcgttagcgt acccagatcg
     4621 taacgtgcct gcgtttccag cccggtatgg cgcgttttgc cacgtgcagt gacggtgtcg
     4681 ttggtctggt tggagtcgta ctgattgtta aagttaatca ggaacagccc catttccgcc
     4741 gtcagcgcgc cgtcgtcgta gcgggtaccg agttcccagg ttcgcgcttt ttccggttcc
     4801 acattgccgc tttgcacagc cttgccaatc tggctgtact gtacggtgcc gaacgagcct
     4861 tcagtgtttg cataaagatt ccagctgtca gtcaggtgat agagcacgtt caacgccgga
     4921 agcggtgcgt tatagctcac ttcttcgtgc gtgcctgtga tggcgttgtt ctggtatgac
     4981 tcgatatgtt cgaaacgcat acccggcgtg atggtccagt tgccgatgtc gattttgtca
     5041 tccagatacc aggcgtgcgc ctcggtgccg gaacgcgtat cgcggtcgta agggcttgag
     5101 ccggacggca actgcccgct gctggtggcg gtgtagtaac gcatttcatg cgttgattca
     5161 ttcacatagc gatagcccac gcccacttcg tgcgcggaag ggccgatcat aaagatctgg
     5221 ctgtagcgtg gctcaatacc gcgcacccag tagttacgcg gcgagagggt gatgcgtttg
     5281 ccttgctcca ggtagccgct gcgcagggtt tgggtgtaga acccctgaat gttgaattta
     5341 tgctggctgt ctggctggaa ctggtagccc aggctcgcca gcttgcgacg accccagaag
     5401 cggtcatacg ggcgggtgga ttgccagcga tcggcgtcgt aatccgcgcg agacaggcca
     5461 ccgggcatgt cggcttcacc gtcgtaatat tgcagcaggc tgttgaaggt gtgcacctca
     5521 tccggcgcat atttgctttt cagcatcagg tcgtcgatgc gggtggcgct gtgctcgcgc
     5581 cagtcactgc cgcgcgtgcc ggagtagagc agcgcggtgc caaaaccgtt gtccgctgtg
     5641 ccgcccacca tcaggttgtg cgtctctttc gggttgtttt gtgaagaggt tgggctgagc
     5701 tgaccttcca cgcccgcctc gataccaaag tcctgcggaa tggcacgggt aacaaagttc
     5761 accacgccgc ccacgctctg cggtccgtaa cgcaccgcac caccaccgcg taccacgtca
     5821 atggcatcca tgttgccgag cgaaacggga gccagtgaaa gctgcggctg accgtaaggg
     5881 gcgaagggga cggggatgcc gtccatcagg acggtcgagc ggctggcgag gcgcgggttc
     5941 aggccccgga tgccaaagtt catcgccagg tcgtggctgc cggtgccgtt gttttccggc
     6001 gcgctgacgc cagggatgcg gttaagtacc tcacgcatgg tggttgcgcc ggttttggcg
     6061 aaatcctcac ggcggatcac gtcacgcgcg ccagcatgtt caaatacgtc gttttcacgc
     6121 gcatcaccca gccagtcgcc gaccacggtc agggcatctt cttttggtgc gggcgcgggc
     6181 tccagcgtcc agctgttatt tcccagcggt tttacctgca gtccgctgcc gtccagcagt
     6241 tgttgcaggc cgctctcgac gtcgtaatcg ccgtgcaggc cgttgctctg cttgccgcgc
     6301 gtcaggctgg cgtcaaccga gagggtaaat ccgctgtgtg cggcatactg attgagcgct
     6361 ttatcgagcg atcccggtgc gatattaacc tgtgcagcaa aagcggaaaa cgagagaccg
     6421 gccagcggca gcaggctcag gcgaatggcg ttaaccaaag gtgttgtttt acgaaaaacg
     6481 cgtaacggcg tcataccttc cccatcatca tttttgttgt gttcagctat gagtcgaacg
     6541 agaagggtaa aaaggacaat cgaaataaga attattttcc ttacagtggt gaaatgttta
     6601 tccagtaccg cgtaatagac tgaattttaa cgggaagcgt ttgcgcgata acgttcagga
     6661 tcgcatcggt atttttcagc gggaacgtcc cgctcaggcg cagcccggca acggcgggat
     6721 cgcagcgcag cacgccgttg cggtaacggg ttagcgtggc tatcacctca cccagcggtt
     6781 tatcgctgaa gctcaggatg tccttcgtcc agcttgtact ctcgtcatcc agcggtttca
     6841 ctgcgccaaa ctcagaggcg ctgaactgca ggctttcacc agcgttcacg atgcgttttt
     6901 gcgcgggggc actggcgaga agcacttcca cggcgtgctg ctgcacgtca agctgcgtga
     6961 aattatcctg ctggcggacg gtaaattctg tccctaaagc agtgagctgg ccctgacggg
     7021 tcaggacacg gaaggggcgt tgcagggcat ctttcgcggt agtaatggcg atttcaccgt
     7081 accagagccg gacggtgcgc tgatgcgcat caaaacgcat atccgccgcg ctttgggtat
     7141 tcagcgtgag cagggagcca tcttccagtt gctgacggct gactgtgcct ttggcggtgc
     7201 ggtaatctgc ccgcagacct tcgccggttt ccgactgcca gagctgccag cctccgccag
     7261 cgccgagcaa cagcagtaat cctttcatca cgtgacggcg ggtgaggcgg gtatcgtgca
     7321 acgcccggct ggcaacgtcg ccaggcacac cgccaagctg gttgcgcagg ttttcaacct
     7381 gctgccaggc ccactggtta tcctgatcct gttcatacca ctgttgccag cgcgcttcct
     7441 gttgtgggct gacgcgctcg ccgcttagca cggcatacca gtgggaagct gaacgcagcg
     7501 cctgacggcg ggaatcggtt aacaaaggat tcataaccca tactccagac ggaacagcag
     7561 gcagtgctcg acggctttcg ccacgtattt tttcacggag ctgatggaaa caccgagttt
     7621 gtgcgcaatc tcgctgtatg tcagaccatc cagttgcgaa agcagaaacg cttcacgtgt
     7681 tttgccgttt agcccgtcca gcatgctgtc gaggagttgt agggtctcga gttggctttc
     7741 gcgttcctca ggtgaaggcg ctcccccctc cggcataagc gccagcatct ccagatacgc
     7801 tttttccagc gcgtttcggc gaaacaggtc caccatcacg cgtttggcga tagtgcagag
     7861 gaaggagcga ggatcgcgga tcgtcgagag cgtttcgctg accattaccc gcaaaaaagt
     7921 gtcctgggca atgtcatctg catcaaaagc agactggagt ttgcgcgtca gccagctttt
     7981 caaccagccg tgatgtgtgc cataaagcga ctcgaatgtt aaggaagctg tggtagtggc
     8041 gcggtcagac atgcggagtg catcaaaagt taattatcac gtagccatat taatatgaga
     8101 atagttatca ttacaattgg aaataaaatt gtttccaata gacattttta acatgttgtt
     8161 tttctaagtg ttataaggta ggtataaaat gggatggagc ctctgcttct ggcatgtgtc
     8221 ggtcagaatg actcatgatg tggtctgcta ttattgacat cctcactgcc ctaaaggata
     8281 gggatttcgg taatgctgtc aacttactga tttagtgtat gatggtgatt ttaaggtgct
     8341 tgcgtggctt ccatttccat cagatgtcct tcctgctccg ctactgaagg cgtggtgcgt
     8401 aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcattgccg taaaacatgg
     8461 caactacagt tcacttacac cgcctctcag cccggtaagc accagaaaat cattgatatg
     8521 gccatgaatg gcgtcggatg tcgcgccagt gcacgcatta tgggcgttgg cctcaacacg
     8581 gttttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata caaccgggca
     8641 gtgatgtgat tgtctgcgct gaaatggacg aacagtgggg ctacgtcggt gctaaatcac
     8701 gtcagcgctg gctgttttac gcgtatgaca ggatacggag gacggttgtg gcgcacgtct
     8761 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
     8821 tcgtggtata gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc
     8881 acgttatcag caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac
     8941 atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca
     9001 aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attacctcct
     9061 cccgaactca aaa
//
LOCUS       DAZOZY010000160         8892 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZOZY010000160 DAZOZY010000000
VERSION     DAZOZY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.161"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..805
                     /gene="gadX"
                     /locus_tag="ACHURQ_003242"
     CDS             <1..805
                     /gene="gadX"
                     /locus_tag="ACHURQ_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR0824959.1"
                     /translation="CLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENF
                     VLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYA
                     EMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASE
                     LLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFI
                     YVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1173..1901
                     /gene="gadW"
                     /locus_tag="ACHURQ_003243"
     CDS             1173..1901
                     /gene="gadW"
                     /locus_tag="ACHURQ_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR0824960.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2264..5377)
                     /gene="mdtF"
                     /locus_tag="ACHURQ_003244"
     CDS             complement(2264..5377)
                     /gene="mdtF"
                     /locus_tag="ACHURQ_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR0824961.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5402..6511)
                     /gene="mdtE"
                     /locus_tag="ACHURQ_003245"
     CDS             complement(5402..6511)
                     /gene="mdtE"
                     /locus_tag="ACHURQ_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR0824962.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6619..6897
                     /locus_tag="ACHURQ_003246"
     CDS             6619..6897
                     /locus_tag="ACHURQ_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0824963.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6898..7425)
                     /gene="gadE"
                     /locus_tag="ACHURQ_003247"
     CDS             complement(6898..7425)
                     /gene="gadE"
                     /locus_tag="ACHURQ_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR0824964.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8224..8796)
                     /gene="hdeD"
                     /locus_tag="ACHURQ_003248"
     CDS             complement(8224..8796)
                     /gene="hdeD"
                     /locus_tag="ACHURQ_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR0824965.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2393 a         2071 c         2068 g         2360 t
ORIGIN      
        1 ttgtctaatt gcgtacgcaa gacataaata tattctcacc atggttaatg gtgaatatcg
       61 ctattttaat ggcggtgacc tggtttttgc ggatgcaagc caaattcgag tagataagtg
      121 tgttgaaaat tttgtattag tatcaaggga tacgctttca ttatttctgc cgatgctcaa
      181 ggaggaggca ttaaatcttc atgcacataa aaaaatttct tcattactcg ttcatcactg
      241 tagcagagat attcctgttt ttcaggaagt tgcgcaacta tcgcagaata agaatcttcg
      301 ctatgcagaa atgctacgta aaagagcatt aatctttgcg ttgttatctg tttttcttga
      361 ggatgagcac tttataccgc tgcttttgaa cgttttgcaa ccgaacatgc gaacacgagt
      421 ttgtacggtt atcaataata atatcgccca tgagtggaca ctagcccgaa tcgccagcga
      481 gctgttgatg agtccaagcc tgttaaagaa aaaattgcgc gaagaagaga catcatattc
      541 acagttgctt actgagtgta gaatgcaacg tgctttgcaa cttattgtta tacatggctt
      601 ttcaattaag cgagtcgcag tgtcctgtgg atatcacagc gtgtcgtatt tcatttacgt
      661 ctttcgaaat tattatggga tgacgcccac agagtatcag gagcgatcgg cgcagggatt
      721 gccgaaccgt gactcggcgg caagtattgt tgcgcaagga aatttttacg gcactgaccg
      781 ttctgcggaa ggaataagat tatagaattt tactcagaca taaaaaaaac ccggcatagg
      841 ggaccgggaa gaggatagtc tgccgtctcc agactaataa accgttataa cactccctgt
      901 tggcacggga aactttgtgc tctcagtaag ttaaatataa cttttactgg aaataagatc
      961 agccattttt ttataaacat aagctatacg ctgtgcgaaa atatattcgt gctgcattta
     1021 cttattatca attaactgtt atgcaaaact actttgtgga taaattttgg tcctaccaaa
     1081 tctggcagtt ttttcgctaa gaaacagtct ggcaacattt cattagtata ctgaaattga
     1141 aataatcgca gtatgaaata taagggataa tcatgactca tgtctgctcg gtgatcctca
     1201 ttcgtcgttc attcgatatt tatcatgaac agcataaaat atcgctgcat aacgagagta
     1261 tcgtgctgct ggagaaaaat ttggcagacg attttgcgtt ttgttcaccg gatacgcgac
     1321 gactggatat cgatgagctg acagtttgcc attacttaca aaatattcgt cagctaccac
     1381 gcaatttagg gttacatagc aaagaccgtc tattaattaa ccagtcaccc cccatgccgc
     1441 tggtgacggc gatttttgat agcttcaatg aatccggggt aaattcaccg atactgagca
     1501 atatgctcta cctttcctgt ttatcgatgt tttctcataa gaaagaactg atccccttac
     1561 ttttcaatag catcagcact gtttcaggaa aagttgaacg ccttattagc tttgatatcg
     1621 ccaaacgttg gtatctgcgc gatatcgcgg aaagaatgta taccagcgag agtctcataa
     1681 aaaaaaagtt gcaggatgaa aatacctgtt tcagtaaaat attactcgct tccaggatgt
     1741 cgatggccag acgattactc gagttacgtc aaattcctct gcatactatt gcagaaaaat
     1801 gtggctatag cagtacgtcg tactttataa acacatttcg acaatattat ggtgtaacgc
     1861 cacatcagtt tgcgcaacat tcgccaggta ccttttcctg acatattttg catttgaata
     1921 ttggtcagga tctcacacct gcttcatgtg aaactcttcc ctgatgattt ctgccgggct
     1981 accggctagt tctctttcgc agtttgccag gcggtatagg tattttcagg aggaaaagac
     2041 tcggcatgtt tgggattatt aagctgacaa ttcatacgat tacgggaatg tgggtcagta
     2101 ttgtgctgtt caaattgatg acgaatggct ggagtggttt ctactttcag tgctgtgtac
     2161 taagcctggt attcctgacg gttagctggt tgttgagtgg tgaatggtta gcaggaaaga
     2221 gtaaggctga accttcacgt tcaaccttac tctcatttac acgttacgct tttttaaagc
     2281 gggcaaagag atgttccact acaacgaaaa agaccggaac gaagtaaatt gccagcactg
     2341 ttgcggcaaa catcccgccc atcacgccgg tacctaccgc gttttgcgcg ccagaaccgg
     2401 caccatgact gataaccagc ggcagcacgc cgagaataaa ggcgagagag gtcatcagga
     2461 ttgggcgtaa acgcatccgc gccgcttcga tgattgcctc tatcggcgtt ttcccttctt
     2521 tctgcatcat ctcaacggca aattcgacaa tcaggatggc gttcttggcg gaaagcccga
     2581 tggtggtcag caaaccaacc tggaagtaga cgtcattact taagccgcgc agatcggtgg
     2641 ccagtaatgc gccaacgacg cctaacggaa caaccaacat caccgagaac ggaattgacc
     2701 agctctcata gagtgcggcg agggcgagga acaccacgac cagtgaaatg gcatacagtg
     2761 caggagcctg gtttgaggat aacgcttcct gatacgacag tccggtccat gagtagccga
     2821 cgcctgccgg aagtttagcg accaggtctg ccataaattt catagcgtca ccggtacttt
     2881 tcccggccgc cgcttcacct aaaatctcca ttgacgggat gccgttgtag cgttccaatc
     2941 gcggtgaacc ataggtccat tcggtagacg agtaggcaga aagcggtgcc atcgtgccag
     3001 aggcgttgcg tacataccat tggttgatgt tatccggcaa catacggaac ggcgtgcctg
     3061 cctggacata cacttttttc acccgcccct ggttgaggaa gtcgttcacg tagctgctgc
     3121 cgaaggcggt ggaaattgtc tggttgatat cagacagcgc cacacccatt gcctcagctt
     3181 tcgcggcgtt gacgttcact ttgaacatcg gcgtatcttc caggccgttc gggcgtaccc
     3241 cggtgacctg gtcaggcgat tgcgctgcca gtgataacag ctcgtttcgt gcctgggtta
     3301 gtttttcgtg ccccaggtta ccgttgtcca gcagttccat atcaaaacct gacgcggtac
     3361 ccagttcagc caccgcgggt aagttgaacg ggaagacgac ggctttattg atactgctta
     3421 acgcaatcat tgcccgctga atgatcgcgg taaccgagtt ttcctcaccg acacgttcag
     3481 accacggctt gagactgata aacgccaggc cgttgttttg cccctggccg ctgaagccaa
     3541 agccgccaac ggtaaacacc gactgaacat tatctttctc ttttgtaaga taataatccg
     3601 tcacctgttg cagcactttc gtggtgttaa ccatggtggc accggaaggt aactgcgcgg
     3661 tggtcataaa taccccctga tcctcttctg gtaagaaaga ggtcggtgta cgcaggaaca
     3721 gcaccgccat cccggcgcaa atcagcaggt agaccaccat gtagcgaccg gtacaacgca
     3781 acagcgagcg ggtgctatcg gtatagtgct gagttgattt ttcaaacagc gtgttgaagc
     3841 gtgcgaacag ggcgttaggt ttgtgaccgc cttccggcgc ggctttcaga atggtggcgc
     3901 acagggcagg ggtcaggctc attgccacaa atactgaaag cagcatggag gagatcagcg
     3961 tgatggagaa ctggcggtag atctccccgg ttgcgccgct cataaaggcc atcggcataa
     4021 acactgcgga aagaacaaca gcaataccga ccagcgcacg ttggatctgc cccatcgatt
     4081 tatgcgtcgc ttccttcggt ggtagcttat cttccgcaat gacacgctcg acgttctcca
     4141 ccaccacgat ggcgtcatcc accagtaacc ctatcgccag caccatcccg aacatagtta
     4201 acgtgttgat ggtgaaaccg accgccgaca agatcgcaaa cgtcccgaga ataaccaccg
     4261 gtacggcaat cgtcgggatg attgtggcac ggaaattctg caaaaacaga tacatgacca
     4321 ggaagacgag gatgatagcc tcaaccagtg ttttaaaaac ttcctgaata gaaatttcga
     4381 taaacggcgt ggtgtcgtaa ggataaaccg tcttcagact tgcggggaaa taggctgata
     4441 agcggttcag ctcctctttg actgcccgcg aggtatccag ggcgtttgct ccggtagcca
     4501 gtttgatggc gatcccggca gcaggtttgc cgttatagcg cgccacggtg gaataatctt
     4561 ccgctccaag ttcgacgcgg gcgacatcac gcagcagcac ttgcgaacca tcttgctgaa
     4621 ctttcaacag gattttgcca aattcttccg gcgtttgcag acgcgtctgc acaatgatcg
     4681 aggcgtttag ttgctggtct gccgcctgtg gcatgccacc cagttgacca ccggaaatct
     4741 ggttgttttg caccttaatc tgggaaataa catcggaagg taccagattg tatttattga
     4801 gtttttgcgg gtccagccag atacgcatgg cgtactcgga accaaagagc tgtacgctac
     4861 caacgcccgc ggtacggctt agcgggtctt tgatattaga cgctacatag tccgcgatat
     4921 cgtactggtt gaggctgccg ttatcagaaa taaacgccgc taccatcaga atattactgc
     4981 tcgacttatc aacgctaatc ccctgctgct gcaccgcttc aggtaatgaa ggcatagcga
     5041 gttgcagttt attttgcacc tgaacctgtg cgatatcagg agatgtccca gtctcgaagg
     5101 tcagagtgat agaggcatta cccgccgcat cactggttga agacatgtac atcaggccat
     5161 caagcccatt catattttgc tcaatcacct gagtgaccga gtcttctacc gtttgcgcat
     5221 cggcacctgg ataggtagcg ctgacggtaa tggtcggtgg cgcaatctgc ggatactgcg
     5281 caaccggtaa gttcatgatc gccagaccac ctgcaagcat cataataatg gcaagtaccc
     5341 aggcaaaaac cgggcgatca ataaaatagt tagccatgaa agtcccctta agcctgcaac
     5401 gttattgttt cgattcggtg ctggcgtttt cctggctgga ggaaattgct cgtgctttga
     5461 tacccggacg aatgcgttgc aaaccggaaa cgatcacccg atcgccagcc tgcaagccag
     5521 aggtgacaac ccactggtcg ccgatggctt tgctggcttc aatttcgcgt agctgcacaa
     5581 catcgtcttt atccagaatg agcgccgttg ctttaccctg ggcgttgtga gtgacgcctt
     5641 cctgcggcac cagtaataca ttctggcggc taccttcatc cactaatgcc gtgacgtaca
     5701 tgccaggcag caagtcacca tttgggttgg ggaaaatcgc ccgtaacgtc acggagcccg
     5761 tggtttcatc caccgtcggg tcggagaatt tcagtgtgcc ggtctggctg tagcgtttac
     5821 cattttccag attgagctgt actggcgtac tgccctgaac ctgtttgatt tgcccactgg
     5881 cgacctcttc tttcatgcgc aagaaatcct gcaccgactg cgtgagatcg acataaatcg
     5941 ggtccagacg ttgtacggta accagcgaat ctgcctgatt agcggtaacg agtgcgccga
     6001 cggtcaccga cgatttcccg ctgacgcccg taatcggcga ggtgacattc gcgtattgca
     6061 gattgatcgt cgcctgttca acagccgctt tggcgacggt gacattggct tctgcttcat
     6121 tcagctgggt gcgcgcggtg tcgtaatcct gacggctaac gtagttggtc ttcagcaacg
     6181 atgcctggcg gttaaaggtg atgcgggcat tgctggcggt agagagcgct ttcgccagcg
     6241 agcctttggc ggagtttagc tcggcctgta aaggtgcagg atcaatctga tacagcgaat
     6301 cgccctggtt cactttatcg ccttcgataa agttgcgttt aatgataata ccgcccacct
     6361 ggggacgtat ctcggcaact tcataaggaa cggttctacc gggcaattcg ctcaacacat
     6421 tgaccgaacc gggggagagt gtgacgacac cgacctcagg cgtcatggcg gcggcgtttt
     6481 ccgccgattt gtcatcgcag gcggtgagca tcgcgccgca gaataacaac ggtattaaca
     6541 gctttcttct tctgttcatt ttagtccctg aaaattcttg agaaataacg gttctttaaa
     6601 ttgcgcgcga ggagagatat gagggattta cagaccagcg gcatagtggg gttgtcagcc
     6661 agtaaggtgg gatacaggca cagtgatcga catggtgagg tcaacgacaa aaaaaatgtt
     6721 gccgttctgc caacagttcc tgccagcatt cgggcaacga agggttctac tggtggatac
     6781 acatacctgg ggaataaagt cgttttagcc tctcctttag taatcaccgg gggtaatgaa
     6841 atcagcatct gcatccctcg tcatgccagc catcaatttc agttgcttat gtcctgacta
     6901 aaaataagat gtgataccca gggtgacgat gtcgctcata cgtcttagtt tcagcttgta
     6961 cataatgttg aatttctgaa tctttaatgt ttgttgcgaa atattgagcg tcgacgtgat
     7021 attgctttca tttttatttt tcagaatcag ttgcataatt ttgaactgat tatgtgaaaa
     7081 acataattgt gaattcttat ggggcaagtg tttaccataa gtaatctcaa gaagattttt
     7141 gatactttct ttgcggctaa caaaataaat aggcgcttta gcttttagtt tactgatgtg
     7201 ataaggtgcc aaaacaataa ttctctcggc atctaatttc tccagaaatt taatcgcttc
     7261 ttcatcaagg atatgattgt gataggtatc aatgataacc ttgaagggtt tattgcctac
     7321 ttttttgatc tctgacaggg aattgatttt tatcatttcg tgattatctt tcaactgcca
     7381 aaagccctgt aaaagaaaag aatctttcgt catgagaaaa atcataactt gctccttagc
     7441 cgttatcgtt tcgaacatgt catccttttc attatttaca tccttgtccg aatcgttgtt
     7501 caatatagta aacgcctata agaactttac ccttcgtgaa ctaatacgtc tattaacact
     7561 aatcaatact tcagatttat ccatcacgca ctccatatat tgatcctaag taagattcct
     7621 ggttgttatc agcttgtaaa tagtaaacaa aaagttaacg tttgcggatt actgcccaag
     7681 aataagttat caataaccag tttgtgatct ctgaagaata ttactaaagt taaaatgtaa
     7741 tccgatttaa atatcgagtc tccttgtttc gacttaagct ggcaattgga ttgccagctt
     7801 tcttttaacg cattgaaatt acctgagtaa ataacgcatt catgtcttca ggtttttata
     7861 tagcgtctgg agacacggat atttatgcaa tgtaatcaac gccagttgca aaaaaatgaa
     7921 cccggattac ttatttaacc gcccatagca tcaacgctta cggaactctt tgttcacttt
     7981 cccacagcac ttttaaaaca ttaaacctga ctacggaaaa tatcagccat gataatgttt
     8041 gaaatggcta atttgccatg gagtgaaaaa aattagatga aattcagtag gttgaaataa
     8101 tcactagcag gtaattattt caatgatagt gcgcaattga tctacaacac tccgtagcgg
     8161 agagagtatt aatcggatca tagtcatatc aagtgactat gatccgggtg acaaccggga
     8221 taattattgc tgcttaacga acaaactggc gaagctgaac aggctggcgg cgctaaatat
     8281 cagttcaatt cccaccagtg tggaaaccag cgttacagac accatcggcg ttgcaccaag
     8341 gaatatccag gcaatgacga tatccagcac accaataacg agctgtagcc agctgccttt
     8401 cattgaacgc tgacgatacc aactcatcag gcgaataacc cctgcaacac agaacaaacc
     8461 ggcaataaat gccgcaatgg caaaaatgcc cagctccggt gcgcggatga agaaatagcc
     8521 gatcaataaa taggcgactg cgacgaggaa accggataat accggccaga aattatgact
     8581 gcggttgctg aataacccga caataagcgc aatgcccgag cagattaata atgcacccac
     8641 tactgtgctt aaaatatcgc cagagacgaa cgggaaactg atacacagca acccgacgat
     8701 aaacagtagc acggcaataa actggattgc tctgcgatgt tttttaagca tctccagatc
     8761 aaacttcaaa attgttgcct tatctatata taacatagaa ccaccctata aaattaagaa
     8821 gaaaatcccc tgctatcaat ctatgccaaa aacgcgtcta agaatgtagt cgatttaata
     8881 aaaatttcct aa
//
LOCUS       DAZOZY010000161         8812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZOZY010000161 DAZOZY010000000
VERSION     DAZOZY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.162"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            95..1493
                     /gene="ulaA"
                     /locus_tag="ACHURQ_003249"
                     /pseudo
     CDS             95..1493
                     /gene="ulaA"
                     /locus_tag="ACHURQ_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            1509..1814
                     /gene="ulaB"
                     /locus_tag="ACHURQ_003250"
     CDS             1509..1814
                     /gene="ulaB"
                     /locus_tag="ACHURQ_003250"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR0824966.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            1824..2285
                     /gene="ulaC"
                     /locus_tag="ACHURQ_003251"
     CDS             1824..2285
                     /gene="ulaC"
                     /locus_tag="ACHURQ_003251"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR0824967.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2299..2949
                     /gene="ulaD"
                     /locus_tag="ACHURQ_003252"
     CDS             2299..2949
                     /gene="ulaD"
                     /locus_tag="ACHURQ_003252"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFR0824968.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2959..3813
                     /gene="ulaE"
                     /locus_tag="ACHURQ_003253"
     CDS             2959..3813
                     /gene="ulaE"
                     /locus_tag="ACHURQ_003253"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR0824969.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            3813..4499
                     /gene="ulaF"
                     /locus_tag="ACHURQ_003254"
     CDS             3813..4499
                     /gene="ulaF"
                     /locus_tag="ACHURQ_003254"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR0824970.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            4596..5147
                     /locus_tag="ACHURQ_003255"
     CDS             4596..5147
                     /locus_tag="ACHURQ_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR0824971.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5222..5497)
                     /gene="yjfY"
                     /locus_tag="ACHURQ_003256"
     CDS             complement(5222..5497)
                     /gene="yjfY"
                     /locus_tag="ACHURQ_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR0824972.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5824..6219
                     /gene="rpsF"
                     /locus_tag="ACHURQ_003257"
     CDS             5824..6219
                     /gene="rpsF"
                     /locus_tag="ACHURQ_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR0824973.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            6226..6540
                     /gene="priB"
                     /locus_tag="ACHURQ_003258"
     CDS             6226..6540
                     /gene="priB"
                     /locus_tag="ACHURQ_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR0824974.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            6545..6772
                     /gene="rpsR"
                     /locus_tag="ACHURQ_003259"
     CDS             6545..6772
                     /gene="rpsR"
                     /locus_tag="ACHURQ_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR0824975.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6814..7263
                     /gene="rplI"
                     /locus_tag="ACHURQ_003260"
     CDS             6814..7263
                     /gene="rplI"
                     /locus_tag="ACHURQ_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR0824976.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(7375..8346)
                     /locus_tag="ACHURQ_003261"
     CDS             complement(7375..8346)
                     /locus_tag="ACHURQ_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0824977.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(8399..>8812)
                     /locus_tag="ACHURQ_003262"
     CDS             complement(8399..>8812)
                     /locus_tag="ACHURQ_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR0824978.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         2123 a         2156 c         2344 g         2189 t
ORIGIN      
        1 ataatcttgt tgtgattact tttgaaaatt agagtgagtg cacaacattc cgggtgtgcg
       61 gaatacccgg ttacctcttc ttcaggagat cgttatggag atcctctaca acatctttac
      121 cgtgtttttt aaccaggtca tgaccaatgc cccgttgttg ctgggtattg tgacctgtct
      181 gggctacatc ctactgcgca aaagtgtcag cgttattatt aaaggcacga ttaaaaccat
      241 aattggtttc atgttgttgc aggcagggtc cggcatcctc accagcacct tcaaaccggt
      301 ggtggcgaaa atgtccgaag tctacggcat taaacggcgc aatttccgat acttacgcgt
      361 cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt tacgcggtgt
      421 tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc ggtattcgta
      481 ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc gttacgttgt
      541 ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg gtttcgctct
      601 actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg gacggctgcg
      661 gtttctccat cggtcaccag caacagtttg catcatggac tgcctataaa gttgcgccgt
      721 tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg ctgaatattt
      781 tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt gccattctgc
      841 tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg acggtctata
      901 tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag ggtgtgcgca
      961 tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg atccctggtg
     1021 cggttctggc gattgactgt gcggctattt atagcttcgc gccgaacgcc gtggtctggg
     1081 gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc gcctgcggtt
     1141 cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc accatcggcg
     1201 tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg gtgatgggga
     1261 tgatcgaaat ctttggttgc gtctgggcgg tgaaacttac cggtatgagt gcctggatgg
     1321 ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc tccatcggta
     1381 tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc gctggccgcg
     1441 cactgcgcgc agaagaagat gcagaaaaac aactggcaga acagtctgct taataaggag
     1501 ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc agttccatga
     1561 tcatgaggat gaaagtggac cagtttttaa cccaatcaaa cattgaccac acggtaaata
     1621 gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc atcgcttcta
     1681 cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc gtgcgcaaca
     1741 tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg catttcccgc
     1801 aggatgtgaa gtaaggacgc gccatgaaat tacgtgattc gctggcggag aataaatcca
     1861 tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc gttgacctgc
     1921 tggtggcgga tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc
     1981 acggtccgta ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg
     2041 gcgttaagaa aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg
     2101 aagataacga cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc
     2161 aggaagtggg catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt
     2221 tacgtgcctg ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag
     2281 cttaagaagg aattgaagat gtcattaccg atgttgcaag tcgcgctgga caaccagact
     2341 atggatagcg cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg
     2401 ggtaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac
     2461 ccgcacaaaa tcgtgctggc agacgccaaa attgccgatg cgggcaaaat tctttctcgt
     2521 atgtgcttcg aagccaacgc tgactgggtg accgtgattt gttgtgcgga tatcaacacc
     2581 gccaaaggtg cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc
     2641 ggttactgga cctgggaaca ggcgcaacag tggcgcgacg caggtattca gcaggtggtt
     2701 tatcaccgca gccgtgacgc gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact
     2761 gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg
     2821 gaagatctgc cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc
     2881 cgtgatgccg cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg
     2941 tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg
     3001 ctccccgccg gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat
     3061 tttgtcgaga tgtcggtaga tgaaactgac gagcgcctgt cgcgcctcga ctggagccgc
     3121 gagcagcgtc tggcgctggt gaatgcgatt gttgaaaccg gcgtgcgtgt gccgtccatg
     3181 tgcctttctg cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag
     3241 gggctggaga ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc
     3301 cagctggcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
     3361 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg
     3421 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
     3481 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
     3541 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
     3601 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
     3661 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
     3721 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gtgattgggt gaaagcgcgc
     3781 atggccaaag caggcacggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg
     3841 aagccaacat ggatctgccg cgctatgggc tggtgacctt tacctggggc aacgtcagcg
     3901 ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca
     3961 tgaaagcaga cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc
     4021 gcccgtcttc cgacaccgcg acgcatctcg aactctaccg tcgttacccg tcgcttggcg
     4081 gcattgtcca tacccactcc actcatgcta ctgcatgggc gcaggcgggg ctggcgatcc
     4141 cggcgttagg cactacgcac gccgactact tctttggcga catcccgtgt acgcgcggat
     4201 taagcgaaga agaggtgcag ggcgagtatg aactgaacac cggcaaagtg attatcgaaa
     4261 cgctgggtga cgccgagccg ctgcatacgc cggggattgt ggtgtatcag cacggtccgt
     4321 tcgcctgggg gaaagatgcc cacgacgcgg tgcataacgc ggtggtgatg gaagaagtgg
     4381 cgaaaatggc gtggattgcg cgcagtatta acccacaact gaatcacatc gacagcttcc
     4441 tcatgaataa acacttcatg cgtaagcacg gtcctaacgc ctattacggg cagaagtaag
     4501 cagtgcgccc cggaattatt acctcctgcg ggatgtaatt ggtctgattc cggggccgct
     4561 tcagttaaaa aatcgtataa acaacaggtg tttttatggc tatcaatgga ttgtttgtca
     4621 gaacggatcc gcaacgtcgc cgctatggcg ttgcgttact tgtgggtgta ctttcaggaa
     4681 ttatttcagc atttgttaaa tggggagcgg aagtcccctt gccaccgcgc acattttccg
     4741 gtggtcgcga tgaatttaac ccgccgtata tctttctacg ggattattta ggtatcgacc
     4801 caacccaaac tgtgtataca ttctcggaac atattatcaa cccggtgatg gtgacgcata
     4861 tcatcttctc actggttttt gcgatagggt attgtattgt cgcggaaatt ttcccgaaag
     4921 tgaaattatg gcaggggata ttagcagggc ttattgtgac agtggcagtc cacggtatat
     4981 tttgcccggc gctgaattta acgccgccgc tcactcaatt gccttttgac gaatatgcct
     5041 ctgaaatcct ggggcatatt ttctggttct ggataataga aattatgcgc cgcgacttac
     5101 gtaatcgcat tactcatgag ccagatccgg aagtcgcaat ccggtgaata accgcataaa
     5161 aaagccccct gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt
     5221 gctagcgata aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca
     5281 gaattacgtg gtagtacgtt gcgccctgcg catctgtctc ttcatttaat gcctgacgcg
     5341 cttcgctttc agtggcgaaa ttatgattga tataaatcac gcctaagctt tgcacatcat
     5401 ccatgtttct ggcctggtgg ttattaattt caatggctgc ccatgtattt gcacttagca
     5461 aaagcacagc cagaagggct aaaactcgac tgaacataga tacctcctcg acggctgact
     5521 ttgtgtgctc tcctctgtga tgatcttctg atttaatttt aatcaatgat aaagaagttg
     5581 atggtgacca tttctgatgc agttgttcaa aaaaacacca tgatgaagtg tgatgaactc
     5641 caaatcagcg gtttagaggt taattgcgaa aggggagatt tatttcggct ctgcccttga
     5701 gtttagcggg gcatacaagt actataacgc gtcatttttc agccgacctt taacacgttc
     5761 cttgcctccc cgggattcgg ctgacccaga caggaggctg aataatccgt aaggagcaat
     5821 tcgatgcgtc attacgaaat cgtttttatg gtccatcctg accagagcga acaggttccg
     5881 ggcatgatcg agcgctacac tgctgccatc actggtgcag aaggcaagat ccaccgtctg
     5941 gaagactggg gccgccgtca gctggcttac ccgatcaaca aactgcacaa agcacactac
     6001 gttctgatga acgttgaagc tccgcaggaa gtgatcgatg agctggaaac taccttccgc
     6061 ttcaacgatg ccgttatccg cagcatggtt atgcgtacca agcacgctgt taccgaagca
     6121 tctccgatgg ttaaagcgaa agacgagcgc cgtgagcgtc gcgatgattt cgcaaacgaa
     6181 accgctgatg atgctgaagc tggggattct gaagagtaat ttctgatgac caaccgtctg
     6241 gtgttgtccg gcaccgtgtg caggactccc cttcgaaagg tcagtccatc aggaattcct
     6301 cactgccagt tcgtgcttga gcatcgttct gtgcaggagg aagccggttt tcaccggcag
     6361 gcgtggtgcc aaatgcccgt tattgttagc ggacacgaaa accaggccat tactcacagt
     6421 ataacggtcg gcagtcgcat aaccgttcag gggttcattt catgccacaa ggcaaagaac
     6481 ggactgagca aaatggtttt gcatgccgag cagattgaat tgatagattc tggagactag
     6541 ccatatggca cgttatttcc gtcgtcgcaa gttctgccgt ttcaccgcgg aaggcgttca
     6601 agagatcgac tataaagata tcgctacgct gaaaaactac atcaccgaaa gcggtaagat
     6661 tgtcccaagc cgtatcaccg gtacccgtgc aaaataccag cgtcagctgg ctcgcgctat
     6721 caaacgcgct cgctacctgt ccctgctgcc gtacactgat cgccatcagt aatcggtcac
     6781 ggtccattaa tacgactttg agaggataag gtaatgcaag ttattctgct tgataaagta
     6841 gcaaacctgg gtagcctggg tgatcaggta aacgttaaag cgggctatgc tcgtaacttc
     6901 ctggtaccgc agggtaaagc tgttccagct accaagaaaa acattgaatt cttcgaagca
     6961 cgtcgcgctg aactggaagc taaactggct gaagttctgg cagctgctaa tgctcgcgct
     7021 gagaaaatca atgcactgga aactgttacc atcgcgtcta aagctggcga cgaaggtaaa
     7081 ctgttcggtt ccatcggtac tcgcgacatc gctgacgctg taactgcagc tggcgttgaa
     7141 gtggctaaga gcgaagttcg tctgccgaac ggcgttctgc gtaccactgg cgagcacgaa
     7201 gtgagcttcc aggttcacag cgaagtattc gcgaaagtga tcgtaaacgt agtagctgaa
     7261 taattcgtta ttcaacgaga cgtaaaaacg ccgaccagtg gtcggcgttt tgcttttgta
     7321 gcgagcactt gcagatatcg ataagggcca caataagcag ccctacgaag ataattactt
     7381 agctgcctga atatcagcca gcattggggc aagggctggg ttgtccttag cgaagtttgc
     7441 gtacattggc tgtactttct cgataaacgg agctttatct acctggtaga ctttcacgcc
     7501 cattttatcc agttttgcaa attcttgttt ctcttcctcg ctccacaagt ctttcatgta
     7561 gctcattgat tctttagctg cttttaatat aactgcctga ttttccgggc taagtttatc
     7621 aaacgcagca ttgctcatca gaaccacatc gggaaccata gtgtgttcgt caaggctgaa
     7681 ggattttgcc acttcaccgt gacgcatggt ggtcagcgcc atcacgctgt tttctgccat
     7741 atcgaccaca ccctgctgta aggctgtata caactcgccg taatccagtg gcgtcgggtt
     7801 accgcccatg actttgatca tttcaactgc gccagggctt ggctggacac ggactttcat
     7861 gcctttcaga tcgtctggtt tcagtactgg tttattgcca tagaaactgc gggcgcctcc
     7921 atcataaaaa gtcagcccga aataaccttt gcttttcgtt gagtcgagga tttttctccc
     7981 gatatcgccc tgcatgacct gataataatg atcacgatcg cggaacaaat aaggcaggct
     8041 aaataattta taggaatttt caaatgcttc catttctgcc gcattggttt ttaccagtgg
     8101 aatagcgcca gaacgaatca gctccattgt ttctcgttga gtacccaatg ttccatttgg
     8161 ataaatacga atagtaatat caccgttaga gtactctttg ttctttttcg caaagaactc
     8221 catcgcttta tgaacaggat gagacttatc ctgattatga ctcagtttta atgtcactgc
     8281 ccaggactgg cttgcggata acataagaac agcaccgata atgccagcgg atacttttat
     8341 tttcatagat gttccttttc taataaatat ggcggcaggt gccgccagtg aatgcgcatc
     8401 agtagcccag tgcttttggc aatgccatac tgatttgcgg aatataggtc accattgcca
     8461 gcaccaatac catcacggca tagaatggca gcataggttt gataatttta ttaatcggta
     8521 atttagatac actacaaccg acaaataaca cactgcctac aggcggagta caaataccga
     8581 tacacatgtt cagtaccatg acaataccaa agtgaatcgg gtccatgccc agttcggtga
     8641 caacaggtaa aaatatcggc gtaaaaatca gtaccgccgg tgtcatatcc ataaaagtgc
     8701 cgataatcaa caggataata ttgataataa gcaggattat tattgggttg tcagaaatag
     8761 tgttaagcaa atcgagaatc aggaagggaa cgtcagcgtt tgacattgcc ca
//
LOCUS       DAZOZY010000162         8797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZOZY010000162 DAZOZY010000000
VERSION     DAZOZY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.163"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..550
                     /gene="ykgH"
                     /locus_tag="ACHURQ_003263"
     CDS             <1..550
                     /gene="ykgH"
                     /locus_tag="ACHURQ_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR0824979.1"
                     /translation="YVNKDNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQ
                     RITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLD
                     EIQIPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSI
                     VISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(793..1488)
                     /locus_tag="ACHURQ_003264"
     CDS             complement(793..1488)
                     /locus_tag="ACHURQ_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR0824980.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1481..2908)
                     /locus_tag="ACHURQ_003265"
     CDS             complement(1481..2908)
                     /locus_tag="ACHURQ_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR0824981.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2919..3638)
                     /locus_tag="ACHURQ_003266"
     CDS             complement(2919..3638)
                     /locus_tag="ACHURQ_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR0824982.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4166..5020)
                     /gene="rclR"
                     /locus_tag="ACHURQ_003267"
     CDS             complement(4166..5020)
                     /gene="rclR"
                     /locus_tag="ACHURQ_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR0824983.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5246..6571
                     /gene="rclA"
                     /locus_tag="ACHURQ_003268"
     CDS             5246..6571
                     /gene="rclA"
                     /locus_tag="ACHURQ_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR0824984.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6680..6916
                     /gene="rclB"
                     /locus_tag="ACHURQ_003269"
     CDS             6680..6916
                     /gene="rclB"
                     /locus_tag="ACHURQ_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR0824985.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6928..7521
                     /gene="rclC"
                     /locus_tag="ACHURQ_003270"
     CDS             6928..7521
                     /gene="rclC"
                     /locus_tag="ACHURQ_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR0824986.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7681..8550)
                     /locus_tag="ACHURQ_003271"
     CDS             complement(7681..8550)
                     /locus_tag="ACHURQ_003271"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR0824987.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
BASE COUNT         2432 a         2110 c         1874 g         2381 t
ORIGIN      
        1 gtatgtcaat aaagataata taaaagtgag ttacagccta aaaataaacc agacaacacc
       61 aggcatactt gttaactgtg atagcaataa taactttgcc tgtcagacca caatgactga
      121 agatgttata cagcgaatta ctacattttt tcacaccagc ccagatgtca agaacagaga
      181 aataaagctg gaatggtcag gagataagag agatttacca actgctgaag cggaaatatc
      241 tcgcgtgcag gcgtctatta tcaaatggta tgcgtcagaa taccataatg gcaggcaagt
      301 tctcgatgag atacaaatac cttcagcaat taacagtgag ctttatacaa aaatgatata
      361 cctgaccagg aactggtcat tgtatccgaa cggtgatggc tgtgtaacta taagctcacc
      421 agaaataaaa aataaatacc ctgctgccat ttgcctggct ctgggatttt ttctaagcat
      481 tgtaatttct gtaatgtttt gccttgtcaa aaaaatggta gatgaatacc aacaaaactc
      541 tgggcagtaa taaacagcat tctgatagag agtgcctgta taaagctgaa gtgacaatga
      601 ccgggaaggg aacccgtttt caggaatgga tgcctgtctt tttcactgta aatatcctgg
      661 cattactaat gccaggatat ttacagaaga gcagcataac catgacggaa cggggttaag
      721 tcaggttgtc cttaacgcta ttttcaacct cgtatgcctt cttcaggttt atgttcagat
      781 ctcatatctc tctcagcaat cctcaataat cagatacacc gctttcaccg gaccatgaac
      841 cccgacgact ttgataagct caatatccgc cgttgagctg ggaccactga tgatgttaat
      901 acatgacggc attcgttcac cggcctgcgc tttctgatgc aatttttctg cgagttgcgc
      961 tacacgcggc aggattgtgc ttttacgcag gataaaaaga gaagattccg ggagcaggct
     1021 caatgaacgc ccgcgctcga cggcggaaaa aagaaccacg cctcccgatt cggttaaacc
     1081 atattcagca tacacgacac ccactttagc ctgctctgcc tgcgagatat tctcggcacc
     1141 tttcgccgga tcccaaacaa cggcattgca ttcctgctgc aaacgttcac taatccccaa
     1201 ttcctccagc ctcgtgtcac cgctaatcac gaccgactga tctcccagct ctttacacag
     1261 acgtattgca gcttctgccg ccttcgcctc gctggtcagc tcacagcgcg tcaacataac
     1321 atcgctggca aactgaataa acgcgtcaca gcgctgctgt tggttaagtt gggtaagccg
     1381 ctcgttagca tagttgttaa gcggcgcatc ttctgcttgc ggttcaagtc gcagcgggcg
     1441 acccagtgcc tgagcaacgt tattcaaaaa ttcgctccga ttatccattc tttttctcct
     1501 gcgcctgatg tttcttaaac caactacgga aactctctcc gtcagcttca ggaagatcgc
     1561 gtgcttccat ccagtcgcta atcgcgccaa atttgagtgg tgttttgccg ccattgataa
     1621 accagcttgc cgcatgagcg ccggccatca tcccgacttt ccacaatcct ggatgactat
     1681 tggcataagc gaacattttt atcgcccgtt gctctgcttt tgcggtgatc cctttttcag
     1741 ccatcacccg acgatgacgc aaaatcagtt ttgacagcgg aatacgcacc ggacacacgc
     1801 tgtcacaagc agtgcataaa gagcaggcgt agggtaaatc tttaaaatct ttatagccgc
     1861 caagtagcgg agaaatcacc gcaccaattg gccctggata aatagagcca tatccatgac
     1921 cgccaatatg gcgatatgcc ggacaagtat tcatacaagc cccgcagcga atacagcgca
     1981 gcacatcccg aaattcagag gccagcacct cagaacgccc gttatcgaca ataaccagat
     2041 gaaactcttc aggaccatca acgtgccccg cttcgcgcgg tcctgtcagc caggtgttgt
     2101 atcccgtcaa acgtgcacca acggcactgc gcgccagcat ggtgatcaat acatctacct
     2161 cggcaaacgt gggggcaata cgctccattc ccatcactgc aatatgcgtt ttaggcagcg
     2221 tggtacacat tcgcgcatta ccttcattgg tcactaggca taccgaacct gtctctgcca
     2281 ccgcgaaatt acagccggta atacctattt cagcactgag gaaatcttcg cggatttttt
     2341 gccggatgaa taaggtcatc gcttcaggcg tttccggccc ctcatagccc agacgttcgt
     2401 gtagcactcg acggatctga tggcgatctt tatgaattgc cgggaccaca acatgagatg
     2461 gcggatcttg atccagctgg agaatatatt cacccagatc ggtttcaatc acctgaatgc
     2521 cagcatcctg caacacatga ttgacaccaa tctcttcggt caccatcgat ttagatttca
     2581 ccaccttccg ggcatttttg cgttgggcaa cctgtaaaat gtagcgggta gcgtcttctt
     2641 tggtttttgc aaaccagacg tgaccgccgt tttgcgtcac tttttctgag agctggtaca
     2701 gataagcgtc gagattactc agaacatgat cacgtatctg ggcggcccga tcgcgccact
     2761 cctcccagtg ccccaattca tcgaccattt tttgccgatt tgccccaata cgctgctgcg
     2821 cgtttgccac cgctttgcgc atgatcggat cttcaatttg ctgacggatg cgtgtcttaa
     2881 aatctgtatt actggttttg atcgacatct ttatatcctc agcggctcat caacacttca
     2941 gcaatatgca tcactttgac tttctgccct tcccgttgta atcgcccact gatgttcagc
     3001 aggcaactca cgtcagcacc aattaaatac tcagggcgga cttccatcag gtgcacaacc
     3061 ttttctttca ccatctcgcc ggatatttcg gccattttga ccgagaacgt gccgccaaat
     3121 ccgcagcagg tatcctgttc agcaaaggtc aacagctcca gtccacgcac atttttcagc
     3181 agcgtaagtg gctcgtcctt cactcccagc ttacgggcca ggctacaaga tgggtgatac
     3241 acagctctcc cttgcaaact ggcacctaca tcgactaccc ctaatttatt aacaataaaa
     3301 gaggtgagat cctgcatacg cgcggcaacc ttttcggcac gtgatgccca ttcgggttca
     3361 tccgccagat acgtcgggta actttttacg gcataggtgc aagagccagc cggtgaaata
     3421 atgggatcgt cgttatcctc cagtgcggcg atcagatttt tcatccctgg aatcgcttct
     3481 ttgatataac cgctattgat cgcaggctga ccgcagcatc cctgtttctc cgggaaattt
     3541 acgcgacagc cgagtttttc cagtagcagc acggagtctc gtgccattct tgatttcagg
     3601 gcgtcaccaa tacaggtgac aaagaaattg acattcacaa ctaatactcc attacttcat
     3661 gcccatttat gcccgatggt tcccctcctt ccgggggatc aatcatcaat aaatatggcg
     3721 ttctttttta attggctagc aacataccac ccctcatttg tgtgacaaat gataatcttt
     3781 catccaaact tcaaatgcaa taactaattt tatttctttc ggcatcgctc acattttatg
     3841 gggaaatcga cgttcatttt tggcataaga aatccattta aaaaggctac atcccttata
     3901 cctattcaga gctacttatt caggggttat tgagatatac actgatggtg tacaaaaaaa
     3961 tcaccgacaa taatcacgta tcaagtaata gtcggggttt tatttaattg aagcaattta
     4021 gtttgcattt ttatttaaaa actgagctag agtccatcag aaaaaaatat ataaagccca
     4081 aaccaccgtt ggaaatagaa atagttgaaa ggctcacaac tcccggaatg cggattatct
     4141 ttgccctgat ttctggcggt tttattcagg gtgcaagctg tctgaccctt tcccgatatt
     4201 ctcccggagt acaaccaaac tcgcggacaa acgccttgtg aaaagatgat tcactggcat
     4261 agcctactga ctcagcgatc accacaacag ggagcatttc ccgggaaaac atctgggccg
     4321 ctatttgtag acgcaacttt gttaatacag ccagcggcgt ggttccggaa acatcacgga
     4381 aaagctgggc aaaacttgcc cgagacatgt gggcgatgct ggccagcgat tcgacggtcc
     4441 aggcgtgtcc tggcatttcc agcatttgct gtattaccgc accaagacgt ggatgcagaa
     4501 gcaaactgag aatgtttttc tctgtattaa cctgtgcaat ccactcacgc accgcgaggg
     4561 taaagaatgt agcgcagatc tggctacaca gtgcatccac tcccggcatc gccgatctgg
     4621 attcctgttg taaaaacggg atcgcctccc tcagccagtt atattccacg ctgtggttaa
     4681 ccggtgctaa aaacagcgtt tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca
     4741 accgaagagt gccgcagaca atacaggtcg attcattatc gacatgactc aggcgatgag
     4801 ctgaattttg tggtagcagg accacatttc ccgggcgtaa tgtaaaaatc tctcccgtcg
     4861 gcatttccag cttcgccgct ccttgcgtta acgcatgcca acgaataacc gataattccc
     4921 cggcaccatg ttgaagctgc cagtcacttc ctaacacgca attcttatcg atcgttcctt
     4981 gtggagcgtt aagcatcaac aaacggctaa gggcatccat atcagactcc tgagtggttt
     5041 ttgattacaa acaccaacaa tcttagtaaa taactaatct aataccatca aatggttaat
     5101 catgattttt gttttgtgat tctgattaag aaaaagaaaa taccagacga taagacaaaa
     5161 tatcaagcgt tgtgaagaaa tggtatttgc tcttttgcgt ctatagtcat gatgtcaaat
     5221 gaacgtgttt cgacaggaaa tcatcatgaa taaatatcag gcagttatta ttggttttgg
     5281 caaggctgga aaaacattag ccgtcacgct ggcaaaagca ggttggcgag tggctctcat
     5341 cgaacaatca aatgcaatgt atggcgggac ctgtattaat atcggctgca tcccaaccaa
     5401 aacattggtt catgacgcac agcagcacac agattttgtc cgtgccatac agcgtaaaaa
     5461 tgaagtggtt aattttttac gtaataagaa ttttcataat cttgcggata tgcccaatat
     5521 cgacgtgatc gacggccagg cggagtttat caataatcat agcctgcgtg ttcatcggcc
     5581 tgagggaaat ctggaaattc atggcgagaa aatttttatt aataccggtg cacaaaccgt
     5641 ggttccgcca attcctggaa ttaccaccac gcctggtgta tatgacagca ccggattact
     5701 taatctaaaa gaattgcctg ggcatttagg tattttgggc ggcggatata ttggcgttga
     5761 gttcgcctct atgttcgcta attttggcag caaggtaacc attttagaag cagcttcgct
     5821 gtttttgcct cgggaagatc gggatattgc tgataatatc gcgacgattt tacgcgatca
     5881 gggcgtcgat attatcctca atgcccatgt ggagcgaatc agtcaccatg aaaatcaagt
     5941 gcaagtgcat agcgagcacg cccaactggc ggtggatgca ctgttaatag cttccggtcg
     6001 tcaaccggct accgcttcgt tacatccaga aaatgccggt atcgcagtaa acgagcgcgg
     6061 ggcaattgtc gttgacaagc aattacatac caccgcagac aatatttggg cgatgggaga
     6121 tgttaccggc gggctgcaat ttacttatat ttcactggat gattaccgca ttgtgcgtga
     6181 tgagttgctg ggagaaggca gacgtagtac tgatgatcgg aaaaatgtgc cttattccgt
     6241 attcatgaca ccgccactgt ccagggttgg tatgacagaa gaacaggcca gagagagtgg
     6301 tgctgatatt caggtggtga cattgcctgt agctgcaatt ccgcgtgcca gagtgatgaa
     6361 tgatactcgt ggggtattaa aagcgattgt tgataataaa acccaacgta tattaggggc
     6421 atcactgctg tgtgttgact cccacgagat gatcaatata gtgaaaatgg tgatggatgc
     6481 cgggctgcct tatagcatat tacgcgatca gatatttact catccgtcga tgagcgaatc
     6541 actcaatgat ctattttcat tagtcaaata aactcaaaat cagacgccag aacaaatatt
     6601 ctggcgtctc agagaaaaga atcttattaa ttctctgtta ctctataact tcttaatcac
     6661 ttcattgatg gtattttata tgtttaaaaa atctgtttta tttgcaacac tattatctgg
     6721 cgttatggaa ttttccacca atgcagatga taaaataatt ctgaaacata tcagcgtctc
     6781 gtcagtatca gcatcaccga cagttctgga ggataccatt gctgatatag ccagaaaata
     6841 taatgcttca tcctggaaag tcacatcgat gcgaattgat aataattcaa ccgcaacagc
     6901 agtattgtat aaataaggat gttcacaatg gaaaaatacc tgcacctgtt aagtcgggga
     6961 gataaaattg gcctgtcatt gattcgtctg agtattgcga ttgtttttat gtggattggg
     7021 ttattaaagt ttgtccctta cgaggcagac agcattacac cattcgtcgc aaacagtcca
     7081 ctaatgtcgt tcttttatga acacccggaa gactataaac agtatctgac tcacgaaggc
     7141 gaatacaaac cagaagcaag ggcatggcaa tcggccaata atacctatgg tttttccaac
     7201 ggtcttggcg tcgtggaggt gattattgct ctgctggttt tggctaatcc tgtcaatcgc
     7261 tggttaggtt tattgggagg actgatggca tataccacgc cgctggtaac actctcattt
     7321 ttaatcacca ccccggaggc atgggtaccc gcattgggtg acgctcatca tggtttccct
     7381 tatttatccg gtgctggtcg cttggtattg aaagatactc tgatgctggc aggtgctgtc
     7441 atgataatgg cagattcggc gcgggaaatt cttaaacaac gcagtaatga atccagttca
     7501 acgttaaaaa ctgaatattg ataatccgaa cactgtctgt tgttcacttt ttcaggcgtc
     7561 aggccccctt tctgatgatg aagcctgacg ccatattacc ggagatatga cagagtgaag
     7621 atcaatatca ctcccctgtt aaaagattct tgtcgtctca ggtaaaatat atgattaata
     7681 tcaatcatgt atttttacag caagacaacc acgaacaaat tcaggattaa aatgttttac
     7741 cgactcccca acataaccca aatcaagcga actaatttgt gccatttctt tctctgtcag
     7801 tgagaaatcc cagatagcaa aattttcttc gatacgctgt tgatgtgtcg atttcggaat
     7861 aacgaccact ccccgttgaa tattccaacg cagaatgact tgagaaatcg atttttgatg
     7921 ggcatctgca atactctgaa gcagattatt ttcaaagggt ttatgtcgtc caccacctaa
     7981 cggagcccat gcttcaggct gcacgttata atacttcatg gttgccagcg cctctggttg
     8041 ggcaaaatag ggatgcaact cgacctggtt gaccatcggt ttaactctta ccgtttcaca
     8101 aaagttcgcc agaacatgag gatagaaatt ggaaacccca attgctttta atttgccctc
     8161 ttcataggca tcttcgagtg cacgccacac actgaaataa tcgcgcatag cctggtgcaa
     8221 taaatagagg tcgaagtact ctagtcccga tttttttaat gatgcttcaa tacctgctgc
     8281 tgccgtatcc tgattcaaca tatcctgccc ccacaattta gacgttataa ataactcatc
     8341 tcgggtacat aaaccttcag atatagcttc acgaacggct tcaccgacgg catcttcatt
     8401 accgtatacc gctgcagtgt cgatgagtcg ataacccgtg cggatagcgt tcaacactga
     8461 ctgtttgcac acgtttttat cggtaacctg aaaaacacca aatcccatca tcggcatttt
     8521 cagattgtta cttaatacgg aaaattccac tgtcagtccc tccattaggc atgaacaatg
     8581 agtctacgtt aaaacataac ctcaaattag tatgtggatt ttgatatcac ttatgcaaaa
     8641 aattcattaa taatgtagga ctgaaacctc tctattttcg gggacaacga agcagacgct
     8701 accagtgctt ttgccttcga ccttgctgtt tttgatacac ttagggccct gggcataacg
     8761 aaaatgtgcg atatcacgaa gaattaatgg agaatgt
//
LOCUS       DAZOZY010000163         8726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZOZY010000163 DAZOZY010000000
VERSION     DAZOZY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.164"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHURQ_003272"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHURQ_003272"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR0824988.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHURQ_003273"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHURQ_003273"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR0824989.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHURQ_003274"
     CDS             complement(4884..5534)
                     /gene="rcsB"
                     /locus_tag="ACHURQ_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR0824990.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHURQ_003275"
     CDS             complement(5551..8223)
                     /gene="rcsD"
                     /locus_tag="ACHURQ_003275"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR0824991.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2137 a         2026 c         2231 g         2332 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacagg tgattagtct ttatctgccg gacttaacgt cactgaagag
     4921 agataattca gcagggcgat atcgttctcg acacccagct tcatcatcgc agatttcttc
     4981 tggctactga tggttttaat actgcggttc agctttttag cgatctcggt caccaggaag
     5041 ccttccgcaa acaggcgcag aacttcactc tcttttggcg agagacgctt gtcaccgtaa
     5101 ccaccagcac tgattttttc caacaggcga gaaacgcttt ccggggtaaa tttcttccct
     5161 ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg caccttgttt cagcacgatc
     5221 ccttcgatat ccagatccaa taccgcacta agaatcgccg ggttgttgtt catagtcaga
     5281 acaatgatcg acaggcttgg gaaatggcgc ttgatgtact tgattaaggt aatgccatcg
     5341 ccgtacttat cgccaggcat ggagagatcg gtaatcaaca catgcgcatc cagtttcggc
     5401 aggttgttga tcagtgctgt agagtcttca aattcgccga caacattcac ccactcaatt
     5461 tgctcaagtg atttgcgaat accgaacaag actatcggat ggtcatcggc aataattacg
     5521 ttcatattgt tcatgtaata ggctaccttg ctacagcaag ctcttgacat aactgtcaat
     5581 gtcgctgatg tatttttcta ttcctggaac atccttctca cgaatcagat gttccagcgt
     5641 ttcacataac tgcttgccgg gtaccagatt tagcatggca aatacgcctt taagacgatg
     5701 agccgtttgg gctaacgcag caaagtcact ggttgctgct tcagtataca gcctcttaac
     5761 atcatccggt actgtgtcta caaagagcgc ataatagccg ctggcatgga gttgcgcatt
     5821 ttcatctccg cccagaggcg attctgtcac ctcttcctgc gccagttgca cttcaattaa
     5881 ttgtaagacc gcttcctgca tagcgttgct catattgaag ttgacgcaca attgaccagg
     5941 cccaatttcc cgtacgccag actcatcatc gcttaaaagc aagccagagg cagtaagatt
     6001 agacggatta tccgttaaaa agatatcata atcttgacta attaatcttt catcgggtgt
     6061 gatacaggtt gcaccccaat tttctaactg gcgagtgaca atattccgaa tttctgccga
     6121 agtaacatcc accattacgc agacatcatc cagtaaacgc tcttcttcct cttcaacttc
     6181 cgggccagcg gcgagcattt tgatatgcac agagtagcgt gtaccaagcc catcccgcgt
     6241 tttgatgttt aaatgaccgc ccagtttacg tgccagttga tcgctcagcc agaatgccag
     6301 cgggtccgcc tttccataac gatcgttttg ggtctggttg ataaacggga agtgcaaatt
     6361 atccatttca tgaatactta cgccttctcc ggtgtccaga atgcggaatg tcaggcggtc
     6421 ttcagaggac tcatcctgat caacctcaag ggtgattttt cccaattgcg ttgaggtcac
     6481 ggcatattgc atcagtagca gcaaaatacg tcgtaaggca tcgcgatcgc cgcggcgcat
     6541 atcgtgtgct ttcagatgat tgttaatcag cagttgcaga cctttacgct tgatggcagg
     6601 caacactgaa ggcacaactt catcaattaa atcctgcacg gagaacagca ccgtctcact
     6661 tttccagcta tcgtccgcaa gcatgttcgc taactgtatt tcatcgacca gccgcaccag
     6721 cacatctgcc tgattcgcca gttgtttgct ttccggggcg ttgagtttag ccgcgctctc
     6781 cgccagggac tgtgcaggtt ctttcagcgc atcgccaatg tttttcataa aggtcatccg
     6841 gccctgctgg tttttctcat acagacgctg cgcctgcttg agtttcttgt ttaccagcac
     6901 ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc ggcgcgacct ggctgcggaa
     6961 catgcggatc tcatacagct cgttattgat cgtcgcctga ataatcccct gatgctgttc
     7021 cgccatggtg gtgatgtttt gcagattcaa atgcggcagc aaatgatcgg caattttgtt
     7081 acttatgaca gtgcggttcg attcctgatc gtgaaccagc aggccgagcg gcagcagtga
     7141 gactatctct tcattgattg cccgtaaaat gcgcaattcg ttattgaccg ccgtgttggg
     7201 tagactttct gtactgcgac tggagaaatg gcggaatgtg gtatagccaa ataacgccag
     7261 cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg ttttgcaacg tatccagcaa
     7321 taaggtgcca taaggaacct gccagaccag gcgcatatcg gtagagttga gtgccgagga
     7381 gatttcaatc ttcgtactgt taaagtggat actgacgcta tccgttcctt ctttctcatt
     7441 atcattgtct cccgttgccg tcgtgtctgg ctcaaggcgg aaactgtcca gcggcatacc
     7501 cggtgggatc aaatcattaa tcggcagatc aaaagccacg accgttgcca gatgtcccgg
     7561 ctggttgaac gtggtacgca aggtaaagta atgaccgttc tgccaggcca ggcggcgcat
     7621 gttagaaaaa ctttcgcgtt catcgagggc gttggcctgt tgcaacattt ctgcacgacg
     7681 tgaatcaaca atgtcactga cggtcgattc tttaaatccg gaggtgagat ctttgagggg
     7741 cagggttgag atcagtacca gactgttatc ctgaccattc aggtaataca tcgaccacgg
     7801 tacattttct gcaccccaca atgtatccag ataagtggac atccgctgag tcatctcaag
     7861 cgttgagttg tcgtgagagc caaagattaa cgcttccgtt ttgcggcgtg gtttctccag
     7921 atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct tcgccggagg agggggaggt
     7981 cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa cgccaggtat cgacgcgctt
     8041 ttggattgcg tgggtaatgt cgacaatctg gtaactttta tctttcagcc aggcgttaac
     8101 ggcgctttgt accattaccc ccatcgtcac cagtaacaca atgatcaaca gtaaaaagaa
     8161 gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg gccgttgtct ctttctgacg
     8221 cattcgtgtt tatgacctgt taaaacttcg cgaagtgata gggcagtata aagggtacag
     8281 tgtgaaattc cagactctta cctgattgat gattctctat tctcccgtga tatttatcat
     8341 cctgcattgg gggaaataga tgtacaagcg ccattttgct gtacaaataa cctacaaaaa
     8401 gcccaacgaa attaattgca aataaacgaa gattgctgga aattatgctg atgttaatta
     8461 tttgtgaaat agttgacaag agttatagtt tttctgtggt agcacagaat aatgaaagtg
     8521 tgtaaagaag ggtaaaaaaa accggatgcg aggcatccgg ttgaaatagg agtaaacaga
     8581 cattcagaaa tgaatgacgg taataaataa agttaatgat gatagcggga gatattctag
     8641 ttgcgagtga aggttttgtt ttgacattca gtgctgtcaa atacttaaga ataagttatt
     8701 gattttaacc ttgaattatt attgct
//
LOCUS       DAZOZY010000164         8715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZOZY010000164 DAZOZY010000000
VERSION     DAZOZY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.165"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(310..573)
                     /gene="rpsT"
                     /locus_tag="ACHURQ_003276"
     CDS             complement(310..573)
                     /gene="rpsT"
                     /locus_tag="ACHURQ_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR0824992.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            676..894
                     /locus_tag="ACHURQ_003277"
     CDS             676..894
                     /locus_tag="ACHURQ_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR0824993.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            902..1843
                     /gene="ribF"
                     /locus_tag="ACHURQ_003278"
     CDS             902..1843
                     /gene="ribF"
                     /locus_tag="ACHURQ_003278"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR0824994.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1886..4702
                     /gene="ileS"
                     /locus_tag="ACHURQ_003279"
     CDS             1886..4702
                     /gene="ileS"
                     /locus_tag="ACHURQ_003279"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR0824995.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4702..5196
                     /gene="lspA"
                     /locus_tag="ACHURQ_003280"
     CDS             4702..5196
                     /gene="lspA"
                     /locus_tag="ACHURQ_003280"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR0824996.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5321..5770
                     /gene="fkpB"
                     /locus_tag="ACHURQ_003281"
     CDS             5321..5770
                     /gene="fkpB"
                     /locus_tag="ACHURQ_003281"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR0824997.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5772..6722
                     /gene="ispH"
                     /locus_tag="ACHURQ_003282"
                     /gene_synonym="lytB"
     CDS             5772..6722
                     /gene="ispH"
                     /locus_tag="ACHURQ_003282"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR0824998.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6788..7702
                     /gene="rihC"
                     /locus_tag="ACHURQ_003283"
     CDS             6788..7702
                     /gene="rihC"
                     /locus_tag="ACHURQ_003283"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR0824999.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7869..8690
                     /gene="dapB"
                     /locus_tag="ACHURQ_003284"
     CDS             7869..8690
                     /gene="dapB"
                     /locus_tag="ACHURQ_003284"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR0825000.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1994 a         2168 c         2472 g         2081 t
ORIGIN      
        1 tgtataggaa tttaattcaa aaatagagtg gtcttaagtt taatacctca atgtgtatca
       61 caatatccac attctttgta ggggagtctg gagattgagt agatattctt gttcagaatg
      121 tatcagccga tggttctacg attcttaagc cacgaagagt tcagaaagtg taacggcatg
      181 tctgcaaaag ccagcatctt ttgactatct ggcaaccggt agtgtgttct ctcacgcatc
      241 ataaaagcag caggcataaa aaaaacccgc ttgcgcgggc tttttcacaa agcttcagca
      301 aattggcgat taagccagtt tgttgatctg tgcagtcagg ttagccttat gacgtgcagc
      361 tttgtttttg tggatcagac ctttagcagc ctgacggtcc acgatcggtt gcatttcgtt
      421 aaatgctttc tgtgcagcag ctttgtcgcc agcttcgata gctgcgtata ctttcttgat
      481 gaaagtacgc atcatagagc gacggcttgc gttgtgctta cgagcctttt cagactgaat
      541 ggcgcgcttc ttagctgatt tgatattagc caaggtccaa ctcccaaatg tgttctatat
      601 ggacaattca aaggccgagg aatatgccct tttagccttc ttttgtcaat ggatttgtgc
      661 aaataagcgc cgttaatgtg ccggcactcg ttacgtagtg atggcgcagg attctaccag
      721 cttgcggggt gtgaatacag cttttccgcg ataaaaattg cagcaggcgg tcactttctt
      781 cccgtgattt gcgccatggc aatgaaaagc cacttctttc tgatttcggt actcaatcgc
      841 cggttaacct tgaccgctgt acaaggtata ctcggacgat tttcactgtt ttgagccaga
      901 catgaagctg atacgcggca tacataatct cagccaggcc ccgcaagaag ggtgtgtgct
      961 gactattggt aatttcgacg gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca
     1021 ggaagaaggg cgcaagcgca acttaccggt gatggtgatg ctttttgaac ctcaaccact
     1081 ggaactgttt gctaccgata aagccccggc aagactgacc cggctgcggg aaaaactgcg
     1141 ttaccttgca gagtgtggcg ttgattacgt gctgtgcgtg cgtttcgaca ggcgtttcgc
     1201 ggcgttaacc gcgcaaaatt tcatcagcga tcttctggtg aagcatttgc gcgtaaaatt
     1261 tcttgccgta ggggatgatt tccgctttgg cgctggtcgt gaaggcgatt tcttgttatt
     1321 acagaaagct ggcatggaat acggcttcga tatcactagt acgcaaactt tttgcgaagg
     1381 tggcgtgcgt attagcagca ccgctgtgcg tcaggcgctg gcggatgaca atctggctct
     1441 ggcagaaagt ttactggggc acccgtttgc catctccggg cgtgtagtcc acggtgatga
     1501 attagggcgc actataggtt tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc
     1561 ggtgaaaggg gtttatgcgg tagaagtgct gggcctcggt gaaaagccgt tacccggcgt
     1621 ggcaaacatc ggaacacgcc caacggttgc cggtattcgc cagcagctgg aagtgcattt
     1681 gttagatgtt gcaatggacc tttacggtcg ccatatacaa gtagtgctgc gtaaaaaaat
     1741 acgcaatgag cagcgatttg cgtcgctgga cgaactgaaa gcgcagattg cgcgtgatga
     1801 attaaccgcc cgcgaatttt ttgggctaac aaaaccggct taagcctgtt atgtaatcaa
     1861 accgaaacac ggaaccgaga atctgatgag tgactataaa tcaaccctga atttgccgga
     1921 aacagggttc ccgatgcgtg gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg
     1981 gactgatgat gatctgtacg gcatcatccg tgcggctaaa aaaggcaaaa aaaccttcat
     2041 tctgcatgat ggccctcctt atgcgaatgg cagcattcat attggtcact cggttaacaa
     2101 gattctgaaa gacattatcg tgaagtccaa agggctttcc ggttatgact cgccgtatgt
     2161 gcctggctgg gactgccacg gtctgccgat cgagctgaaa gtagagcaag aatacggtaa
     2221 gccgggtgag aaattcaccg ccgccgagtt ccgcgccaag tgccgcgaat acgcggcgac
     2281 ccaggttgac ggtcaacgca aagactttat ccgtctgggc gtgctgggcg actggtcgca
     2341 cccgtacctg accatggact tcaaaactga agccaacatc atccgcgcgc tgggcaaaat
     2401 catcggcaac ggtcacctgc acaaaggcgc gaagccagtt cactggtgcg ttgactgccg
     2461 ttctgcgctg gcggaagcgg aagttgagta ttacgacaaa acttctccgt ccatcgacgt
     2521 tgcttttcag gcggtcgatc aggatgcact gaaagcaaaa tttgccgtaa gcaacgttaa
     2581 cggcccaatc tcgctggtga tctggaccac tacgccgtgg actctgcctg cgaaccgcgc
     2641 aatctctatt gcacctgatt tcgactatgc gctggtgcag atcgacggtc aggccgtgat
     2701 tctggcgaaa gatctggttg aaagcgtaat gcagcgtatc ggcgtgaccg attacaccat
     2761 tctcggcacg gtaaaaggtg cggagcttga gttgctgcgc tttacccatc cgtttatggg
     2821 cttcgacgtt ccggcaatcc tcggcgatca cgttacccta gatgccggta ccggtgccgt
     2881 tcacaccgcg cctggccacg gcccggacga ctatgtgatc ggtcagaaat acggcctgga
     2941 aaccgctaac ccggttggcc cggacggcac ttatctgccg ggcacttatc cgacgctgga
     3001 tggcgtgaac gtcttcaaag cgaacgacat cgtcgttgcg ctgctgcagg aaaaaggcgc
     3061 gctgctgcac gttgagaaaa tgcagcacag ctatccgtgc tgctggcgtc acaaaacgcc
     3121 gatcatcttc cgcgcgacgc cgcagtggtt cgtcagcatg gatcagaaag gtctgcgtgc
     3181 gcagtcactg aaagagatca aaggcgtgca gtggatcccg gactggggcc aggcgcgtat
     3241 cgagtcgatg gttgctaacc gtcctgactg gtgtatctcc cgtcagcgca cctggggcgt
     3301 accgatgtca ctgttcgtgc acaaagacac ggaagagctg catccgcgta ctctcgaact
     3361 aatggaagaa gtggcaaaac gcgttgaagt tgacggcatc caggcgtggt gggatcttga
     3421 tgcgaaagag atcctcggcg atgaagctga tcagtacgtg aaagtgccgg acacattgga
     3481 tgtatggttt gactccggat ctacccactc ttctgttgtt gacgtgcgtc cggaatttgc
     3541 cggtcacgca gcggacatgt atctggaagg ttctgaccag caccgtggtt ggttcatgtc
     3601 ttccctaatg atctccaccg cgatgaaagg caaagcgccg tatcgtcagg tactgaccca
     3661 cggctttacc gtggatggtc agggtcgcaa gatgtctaaa tccatcggca ataccgtttc
     3721 gccgcaggat gtgatgaaca aactgggcgc ggatattctg cgtctgtggg tggcatcaac
     3781 cgactacacc ggtgaaatgg ccgtttctga cgagatcctg aaacgtgctg ccgatagcta
     3841 tcgtcgtatc cgtaacaccg cgcgcttcct gctggcaaac ctgaacggtt ttgatccagc
     3901 aaaagatatg gtgaaaccgg aagagatggt ggtactggat cgctgggccg taggttgtgc
     3961 gaaagcggca caggaagaca tcctcaaggc gtacgaagca tacgatttcc acgaagtggt
     4021 acagcgtctg atgcgcttct gctccgttga gatgggttcc ttctacctcg acatcatcaa
     4081 agaccgtcag tacaccgcca aagcggacag tgtggcgcgt cgtagctgcc agactgcgct
     4141 gtatcacatc gcagaagcgc tggtgcgctg gatggcacca atcctctcct tcaccgctga
     4201 tgaagtgtgg ggctacctgc cgggcgaacg tgaaaaatac gtcttcaccg gtgagtggta
     4261 cgaaggcctg tttggcctgg cagacagtga agcgatgaac gatgcgttct gggacgagct
     4321 gttgaaagtg cgtggcgaag tgaacaaagt cattgagcaa gcgcgtgccg acaagaaagt
     4381 gggtggctcg ctggaagcgg cagtaacctt gtatgcagaa ccggaactgt cggcgaaact
     4441 gaccgcgctg ggcgatgaat tacgatttgt cctgttgacc tccggcgcta ccgttgcaga
     4501 ctataacgac gcacctgctg atgctcagca gagcgaagta ctcaaagggc tgaaagtcgc
     4561 gttgagtaaa gccgaaggtg agaagtgccc acgctgctgg cactacaccc aggatgtcgg
     4621 caaggtggcg gaacacgcag aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg
     4681 tgaaaaacgt aagtttgcct gatgagtcaa tcgatctgtt caacagggct acgctggctg
     4741 tggctggtgg tagtcgtgct gattatcgat ctgggcagca aatacctgat cctccagaac
     4801 tttgctctgg gggatacggt cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac
     4861 tatggcgcgg cgtttagttt ccttgccgat agcggcggct ggcagcgttg gttctttgcc
     4921 ggtattgcga ttggtattag cgtgatcctg gcagtgatga tgtatcgctc gaaggccacg
     4981 cagaagctaa acaatatcgc ttacgcgctg attattggcg gcgcgctggg caacctgttc
     5041 gaccgcctgt ggcacggctt cgttgtcgat atgatcgact tctacgtcgg cgactggcac
     5101 ttcgccacct tcaaccttgc cgatactgcc atctgtgtcg gtgcggcact gattgtgctg
     5161 gaaggttttt tgccttctag agcgaaaaaa caataataaa ccctgccgga tgcgatgctg
     5221 acgcatctta tccggcctac agattgctgc gaaatcgtag gccggataag gcgtttacgc
     5281 cgcatccggc aaaaatcctc aaatataaga gcaaacctgc atgtctgaat ctgtacagag
     5341 caatagcgcc gtcctggtgc acttcacgct aaaactcgac gatggcacca ccgccgagtc
     5401 tacccgcaac aacggtaaac cggcgctgtt ccgcctgggt gatgcttctc tttctgaagg
     5461 gctggagcaa cacctgttgg ggctgaaagt gggcgataaa accaccttct cgttggagcc
     5521 agatgcggcg tttggcgtgc cgtcaccgga cctgattcag tacttctccc gccgtgaatt
     5581 tatggatgca ggcgagccag aaattggcgc aatcatgctt tttaccgcaa tggatggcag
     5641 tgagatgcct ggcgtgatcc gcgaaattaa cggcgactcc attaccgttg atttcaacca
     5701 tccgctggcc gggcagaccg ttcattttga tattgaagtg ctggaaatcg atccggcatt
     5761 ggaggcgtaa catgcagatc ctgttggcca acccgcgtgg tttttgtgcc ggggtagacc
     5821 gcgctatcag cattgttgaa aacgcgctgg ccatttacgg cgcaccgata tatgtccgtc
     5881 acgaagtggt acataaccgc tatgtggtcg atagcttgcg tgagcgtggg gctatcttta
     5941 ttgagcagat tagcgaagta ccggacggcg cgatcctgat tttctccgca cacggtgttt
     6001 ctcaggcggt acgtaacgaa gcaaaaagcc gcgatttgac ggtgtttgat gccacctgtc
     6061 cgctggtgac caaagtgcat atggaagtcg cccgcgccag ccgtcgtggc gaagagtcga
     6121 ttctcatcgg ccacgccggg catccggaag tggaaggtac aatgggccag tacagtaacc
     6181 cggaaggggg aatgtatctg gtcgaatcgc cggacgatgt gtggaaactg acggtcaaaa
     6241 acgaagagaa gctctccttt atgacccaga ccacgctgtc ggtggatgac acgtctgatg
     6301 tgatcgacgc gctgcgtaaa cgcttcccga aaattgtcgg tccgcgcaaa gatgacatct
     6361 gctacgccac gactaaccgt caggaagcgg tacgcgccct ggcagaacag gcggaagttg
     6421 tgttggtggt cggttcgaaa aactcctcca actccaaccg tctggcggag ctggcccagc
     6481 gtatgggcaa acgcgcgttt ttgattgacg atgcgaaaga tatccaggaa gagtgggtga
     6541 aagaggttaa atgcgtcggc gtgactgcgg gcgcatcggc tccggatatt ctggtgcaaa
     6601 atgtggtggc acgtttgcag cagctgggcg gtggtgaagc cattccgctg gaaggccgtg
     6661 aagaaaacat tgttttcgaa gtgccgaaag agctgcgtgt cgatattcgt gaagtcgatt
     6721 aagtcattag cagcctaagt tatgcgaaaa tgccggtctt gttaccggca ttttttatgg
     6781 agaaaacatg cgtttaccta tcttcctcga tactgacccc ggcattgacg atgccgtcgc
     6841 cattgccgcc gcgatttttg cccccgaact cgacctgcaa ctgatgacca ccgtcgcggg
     6901 taatgtctcg gttgagaaaa ccacccgcaa cgccctgcaa ctgctgcatt tctggaatgc
     6961 ggagattccg ctcgcccagg gagccgctgt gccgctggta cgcgcaccgc gtgatgcagc
     7021 atctgtgcac ggcgaatcgg gaatggctgg ctacgacttt gttgagcaca accgaaagcc
     7081 aatcgggata ccagcgtttc tggcgattcg tgatgccctg atgcgtgcac cagagcctgt
     7141 taccctggtg gccattggcc cgttaaccaa tattgcgctg ttactttcac aatgcccgga
     7201 atgtaaaccg tatattcgtc gtctggtgat catgggcggt tcttctggtc gcggcaactg
     7261 tacaccaaat gccgagttta atatcgctgc cgatccagaa gcggctgcct gtgtcttccg
     7321 tagcggaatc gaaatcgtca tgtgcggtct ggatgtcacc aatcaggcaa tattaacccc
     7381 tgactatctc gctactttgc cggagttgaa tcgcaccgga aaaatgcttc atgcactgtt
     7441 tagccactac cgtagcggca gtatgcaaag cggtctgcga atgcacgatc tctgcgccat
     7501 cgcctggctg gtgcgcccgg acttattcac cctcaagccg tgttttgtcg cggtagaaac
     7561 tcaaggcgaa tttacctcag gcacgacggt ggttgatatc gacggttgcc tgggcaaacc
     7621 agccaatgta aaggtggcat tggatctgga tgtgaaaggc ttccagcagt gggtggctga
     7681 ggtgctggct ctggcgtcgt aacctgtcac atgttatcgg catgcagtca ttcatcgact
     7741 catgcctttc actgatatcc ctccctgttt atcattaatt tctaattatc agcgtttttg
     7801 gctggcggcg tagcgatgcg ctggttactc tgaaaacggt ctatgcaaat taacaaaaga
     7861 gaatagctat gcatgatgca aacatccgcg ttgccatcgc gggagccggg gggcgtatgg
     7921 gccgccagtt gattcaggcg gcgctggcat tagaaggcgt gcagctgggc gcggcgctgg
     7981 agcgcgaagg atcttcttta ctgggcagcg acgccggtga gctggctggt gcagggaaaa
     8041 caggcgttac cgtgcaaagc agcctcgatg cggtaaaaga tgattttgat gtgtttatcg
     8101 attttacccg tccggaaggt acgctgaacc atctcgcttt ttgtcgccag catggcaaag
     8161 ggatggtgat cggcactacg gggtttgacg aagccggtaa acaggcgatt cgtgacgccg
     8221 ccgccgatat tgcgattgtc tttgccgcca attttagcgt tggcgttaac gtcatgctta
     8281 agctgctgga gaaagcagcc aaagtgatgg gtgactacac cgatatcgaa attattgaag
     8341 cacatcatag acataaagtt gatgcgccgt caggcaccgc actggcaatg ggagaggcga
     8401 tcgcccacgc ccttgataaa gatctgaaag attgcgcggt ctacagtcgt gaaggccaca
     8461 ccggtgaacg tgtgcctggc accattggtt ttgccaccgt gcgtgcaggt gacatcgttg
     8521 gtgaacatac cgcgatgttt gccgatattg gcgagcgtct ggagatcacc cataaggcgt
     8581 ccagccgtat gacatttgct aacggcgcgg taagatcggc tttgtggttg agtggtaagg
     8641 aaaatggtct ttttgatatg cgagatgtac ttaatctcaa taatttgtaa ccacaaaata
     8701 tttgttatgg tgcaa
//
LOCUS       DAZOZY010000165         8696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZOZY010000165 DAZOZY010000000
VERSION     DAZOZY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.166"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHURQ_003285"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHURQ_003285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR0825001.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHURQ_003286"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHURQ_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR0825002.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHURQ_003287"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHURQ_003287"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR0825003.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHURQ_003288"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHURQ_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR0825004.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHURQ_003289"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHURQ_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR0825005.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHURQ_003290"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHURQ_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR0825006.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHURQ_003291"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHURQ_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR0825007.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHURQ_003292"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHURQ_003292"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0825008.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHURQ_003293"
     CDS             6614..7768
                     /locus_tag="ACHURQ_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR0825009.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHURQ_003294"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHURQ_003294"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR0825010.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2314 g         2052 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttct gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtg
//
LOCUS       DAZOZY010000166         8639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZOZY010000166 DAZOZY010000000
VERSION     DAZOZY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.167"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..742
                     /locus_tag="ACHURQ_003295"
     CDS             <1..742
                     /locus_tag="ACHURQ_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0825011.1"
                     /translation="QHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMD
                     GNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKV
                     LTSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIR
                     SNAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYT
                     MGLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            1004..2626
                     /locus_tag="ACHURQ_003296"
     CDS             1004..2626
                     /locus_tag="ACHURQ_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR0825012.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTINDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2816..2929
                     /gene="yhiY"
                     /locus_tag="ACHURQ_003297"
     CDS             2816..2929
                     /gene="yhiY"
                     /locus_tag="ACHURQ_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR0825013.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2992..4059
                     /locus_tag="ACHURQ_003298"
     CDS             2992..4059
                     /locus_tag="ACHURQ_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0825014.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4056..6791
                     /gene="rbbA"
                     /locus_tag="ACHURQ_003299"
     CDS             4056..6791
                     /gene="rbbA"
                     /locus_tag="ACHURQ_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR0825015.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6791..7915
                     /locus_tag="ACHURQ_003300"
     CDS             6791..7915
                     /locus_tag="ACHURQ_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR0825016.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7988..8260
                     /locus_tag="ACHURQ_003301"
     CDS             7988..8260
                     /locus_tag="ACHURQ_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFR0825017.1"
                     /translation="MRTKVQALRKKKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIK
                     EGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            8257..8616
                     /locus_tag="ACHURQ_003302"
     CDS             8257..8616
                     /locus_tag="ACHURQ_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR0825018.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
BASE COUNT         2236 a         1978 c         2249 g         2176 t
ORIGIN      
        1 tcaacatgtt tttttaaagg acgatgcgat aaccccttgt atcttccttg gggatcatac
       61 aggcgataga tttagtacta tttttggtga taaatatatc cttactttgc taaattctat
      121 gcgaaatatg gatggtaata aagatagccg tattaataaa aatgtcatcg ttctggctgg
      181 aaatcatgaa atcaatttta atggtaacta tactgcacga ctagaaaatt ataaactctc
      241 tgccggtgat acgtataact taattaaaac gctcgatgtt tgtcattatg actcagaaac
      301 gaaagtttta accagtcatc acggaataat aagaaatgaa gaaaatgaat gttattgtct
      361 cggtgctttg caggttccat ttaatcagat gaaagaccca attgatcctg aggaattagc
      421 aaatatattt aataaaaaac ataaacaaca catggatgat cgtcttatcc atctaattag
      481 aagcaatgct gtagcatcca cacctgtata tgaaaattat tttaataaca ctactgcttt
      541 cagaccaaaa cctgaagata tcttcaaatg tggacagacg ctcaaaaaaa ctaaacagaa
      601 atatggccat tatggattag gggtagatca gcatcaaaaa attgataatt ataccatggg
      661 gctaaattcc tggaagatag cacctaacga acgtggtgat aaaaaaggtg tcccagggct
      721 tagttgtttt caacctcaat aaaacttcat gagtgctgtg gtatctcttt ttaattttgc
      781 cagtatttca taggttttct taatcgattg tagaggcatg ttctgatgac gcctctacaa
      841 tctttaggga ctttctctgc tatgagataa gaaatttcat attatgcttt aaggctgaat
      901 gatttgtgca aaatataatt attttcccat cgaacatgag gtgtatgtca taagattaaa
      961 tgttaactat gtattcctcg cggtaaataa tatgaggtaa aagatgaaaa ttggaactgt
     1021 ggcaggcact aacgacagta caacgacaat cacaacaaat gatatggtgc aagagcatgt
     1081 taccaacttc actaaagaac tgtttgggta tattgcaaat ggtataggtg atgatatatc
     1141 aagtattgcc aggacaatgc tcggtgaggt tgtggaaaaa atagatgatt ggcaaataga
     1201 acggttccaa caatctattc aagatgacaa aatttcattt accatacaaa cgaatcattc
     1261 agaaaaatat agtatgctat cgggcatgcg cgctcatatt ctacgtagga ataactgtta
     1321 tcagtttatt gtgacaatta atagtaaaaa ttacggttgc cctcttgata ataccgatat
     1381 taattggtgc agcattgttt atttacttaa taatatgaca ataaatgata atgctaatga
     1441 tgttgcagtt actgagtcgt acaaacccat ctggaactgg aaaatatcgc aatacaatgt
     1501 atttgatatt aaattcgaaa caatgataaa accccagttc gctgacagaa cctatttctc
     1561 caattgttca cctgttgatc ccacaagtac cagaccgacc tattttggcg ataccgatgg
     1621 ctcagtcggt gcagttttgt acgcgctttt tgcaacggga catttgggaa tcatggctga
     1681 aggagaaaat ttccttagcc agttgcttaa tatagaagat gaagttctca acgtattgct
     1741 aagggagaac tttaatgagc aacttgacac taatgttaat ataataatca gtatcctgaa
     1801 cagaagagac attgttttag aaagtcttca gccttatttg gttattaata aagacgcagt
     1861 cacgccatgt acgtttctgg gcgaccagac tggcgatcga tttagtaata tctgtggcga
     1921 taaatttatc atcgatttat taaaacgcat aatgagtatc aatgaaaacg ttcatgtatt
     1981 agctggaaat catgaaacga attgcaatgg taactatatg cagaacttca cgcgcatgaa
     2041 accacttgat gaggatacct actctgggat aaaagattat cctgtatgtt tctatgaccc
     2101 caaatacaaa ataatggcaa atcatcatgg gataactttt gatgatcagc gaaaacgtta
     2161 cattatagga ccgataacag tctctagtga tgaaatgacc aatgctctcg atccggtcga
     2221 attagcggca attatcaata aaaagcacca tgctgtaatt aatggcaaaa agtttaaaaa
     2281 tagccgggct atctcttgtc gatcctttaa tcgttatttt tctgtttcga cagattacag
     2341 acctaaactt gaagcactat tagcatgctc acaaatgtta ggtataaatc aagttgttgc
     2401 acataacggt aatggtggaa gagaatgtat aggtgaaacg gggacagttt taggtcttaa
     2461 cgcccgtgac agtaaacatg ctggaagaat gttcagcatg cataattgcc aaattaaccc
     2521 tggtgcaggg cctgagataa ccactccctg gaagtcttac cagcatgaaa aaaacagaaa
     2581 tggacttatg cctttgatca gaaggcgcac aatgttacag ctctgaacca tagacaacat
     2641 gaatattaat gttactatct ggttgagact gttgataaaa cgtaaaaagg aatgctctgt
     2701 tcctgcaact gaacaggcgt gaaacgctat ccaacaggat ggataccgtt ttgtcagcca
     2761 gttttataga tcacacttat gagtatgcgt gtttcattta cggccaggaa tgctgatgat
     2821 gacaacatta ctacccgttt tcactaagcc ttccccgttg gcgctcaatg ctctgcgcgc
     2881 tggtcgtatt tgtcgtttcc ttcttatccc ggatggaaga atccgctgaa cgtcgttcgg
     2941 cggcagttgt aacacgcgtc gtttgcttgc agggattatg gcgggtcgat tatggataag
     3001 agtaagcgcc atctggcgtg gtgggttgtc gggttactgg tggtggctgc cgtcgtggcg
     3061 tggtggctgt tgcgcccggc gggtgtgccg gaaggctttg ctgtcagcaa tgggcgcatt
     3121 gaagcgacgg aagtggatat cgccagcaaa attgccgggc gtatcgacac cattctggtg
     3181 aaagaaggcc agtttgttcg cgaaggcgaa gtgctggcga agatggatac tcgcgtgttg
     3241 caggagcagc gactggaagc catcgcgcaa atcaaagaag cacaaagtgc cgttgctgcc
     3301 gcgcaggctt tgctggagca acggcaaagc gaaactcgtg ccgcgcagtc gctggttaat
     3361 caacgccagt ccgaactgga ctccgtggct aaacgtcata cgcgttcccg ctcgctcgcc
     3421 caacgagggg ctatttctgc gcaacagctg gatgacgatc gcgccgccgc tgagagcgcc
     3481 cgagctgcgc tggaatcggc aaaagctcag gtatcggctt ctaaagcggc tatagaagcg
     3541 gcccgtacca atatcattca ggcccaaact cgcgtcgaag ccgcgcaagc cactgaacgg
     3601 cgtattgccg cagatatcga tgacagtgaa ctgaaagccc cgcgtgacgg acgcgtgcag
     3661 tatcgggttg ctgagccagg ggaagtgctg gcggcaggcg gtcgggtgct gaatatggtc
     3721 gatctcagcg acgtctatat gactttcttc ctgccaaccg aacaggcggg cacgctgaaa
     3781 cttggcggtg aagcccggct gatcctcgat gccgcgccag atctgcgtat tccggcgacc
     3841 atcagttttg tcgccagtgt cgcccagttc acgccaaaaa ccgtcgaaac cagcgatgaa
     3901 cggctgaaac tgatgttccg cgtcaaagcg cgtatcccac cggaattact ccagcagcat
     3961 ctggaatatg tcaaaaccgg tttaccgggc gtagcgtggg tgcgggtgaa tgaagaactt
     4021 ccgtggcctg acgacctcgt ggtgaggttg ccgcaatgac gcatctggaa ctggttcccg
     4081 tcccgcctgt cgcgcaactg gcgggcgtga gccagcatta tggaaaaaac gttgcgctga
     4141 acaatattac tctcgatatt ccggcccgct gtatggtcgg gctgattggt ccggatggcg
     4201 tcgggaagtc gagcttattg tcgttgattt ccggtgcccg cgtcattgag cagggcaatg
     4261 tgatggtgct gggcggcgat atgcgcgacc cgaggcatcg ccgcgacgtc tgcccgcgca
     4321 tcgcctggat gccgcagggg ctgggcaaaa acctctacca caccttgtcg gtgtatgaaa
     4381 acgtcgattt tttcgctcgc ctgttcggtc acgacaaagc ggagcgggaa gtgcgaatta
     4441 atgagctgct gaccagcacg gggttagcac cgtttcgcga tcgtccggca gggaaactct
     4501 ccggcgggat gaagcaaaaa cttgggctgt gctgcgcgtt aatccacgac ccggaactgt
     4561 tgatccttga tgagccaaca acgggggttg acccgctctc ccgctcccag ttctgggatc
     4621 tgatcgacag tattcgccag cggcagagca atatgagcgt gctggtcgcc accgcctata
     4681 tggaagaggc cgaacgcttc gactggctgg tagcgatgaa tgccggagaa gtgctggcaa
     4741 ctggcagcgc cgaagagcta cggcagcaaa cgcaaagcgc tacgctggaa gaagcattta
     4801 taaatctgtt accgcaagcg caacgccagg cgcatcaggc ggtagtgatc ccaccgtatc
     4861 aacctgaaaa cgcagagatt gccatcgaag cgcgcgatct gaccatgcgt tttggttcct
     4921 tcgttgccgt tgatcacgtt aatttccgca ttccacgcgg ggagattttt ggttttcttg
     4981 gttcgaacgg ctgcggtaaa tccaccacca tgaaaatgct caccggactg ctgcccgcca
     5041 gcgaaggtga ggcgtggctg ttcgggcaac cggttgatcc aaaagatatc gatacccgcc
     5101 gtcgggtggg ctatatgtcg caggcgtttt cgctctataa cgaactcacc gtgcggcaaa
     5161 accttgagtt acatgcccgt ttgtttcaca tcccggaagc ggaaattccc gcaagagtgg
     5221 ctgaaatgag cgagcgtttt aagctcaacg acgttgaaga tgttctgccg gagtcattgc
     5281 cgctcggcat tcgccagcgg ctttcgctgg cggtggcggt gattcatcgc ccggagatgt
     5341 taatcctcga tgagcctact tctggtgtcg atccggtggc gagggatatg ttctggcagt
     5401 tgatggtcga tctctcgcgc caggacaaag tgactatctt catctctacc cactttatga
     5461 acgaagcgga acgttgcgac cgcatctcac tgatgcacgc cggaaaagtg cttgccagcg
     5521 gtacaccgca ggaactggtt gagaaacgcg gagccgccag tctggaagag gcatttatcg
     5581 cctatttgca ggaagcggca gggcagagca acgaagccga agcgccgccc gtggtacacg
     5641 acaccaccca cgcgccgcgt cagggattta gcctgcgccg tctgtttagc tacagccgcc
     5701 gcgaagcgct ggaactgcga cgcgatccag tacgttcgac gctggcgctg atgggaacgg
     5761 tgatcctgat gctgataatg ggttacggca tcagtatgga tgtggaaaac ctgcgctttg
     5821 cggtgctcga ccgcgaccag accgtcagta gccaggcgtg gacactcaac ctctccggtt
     5881 cccgttactt tatcgaacag ccgccgctca ccagttatga cgagcttgat cgtcggatgc
     5941 gtgcgggcga tatcacggtg gcgattgaga tcccgcccaa tttcgggcgc gatatcgcgc
     6001 gtggtacgcc tgtggaactc ggcgtctgga tcgacggagc gatgcccagt cgtgccgaaa
     6061 cggtaaaagg ttacgtgcag gcaatgcacc agagttggct tcaggatgtg gcgagccgac
     6121 aatcgacacc cgccagccaa agcgggctga tgaacattga gacgcgctat cgctataacc
     6181 cggacgtaaa aagcctgcca gcgattgttc cggcggtgat cccgcttctg ctgatgatga
     6241 tcccgtcaat gctaagcgcc cttagcgtgg tgcgggaaaa agagctaggg tcgattatca
     6301 acctttacgt gacccccacc acccgtagcg aatttttgct tggcaaacag ctgccgtaca
     6361 tcgcgctggg gatgctgaac tttttcctgc tctgcggcct gtcggtgttt gtgtttggcg
     6421 taccgcataa aggcagtttc ctgacgctca ccctggcggc gctgctgtat atcatcattg
     6481 ccaccggaat ggggctgctg atctccacct ttatgaaaag ccagattgcc gccattttcg
     6541 gaacggcgat tatcacgttg atcccggcga cacagttttc cgggatgatc gatccggtag
     6601 cttcgctgga agggcctgga cgttggatcg gcgaggttta cccgaccagt cattttctga
     6661 ctatcgcccg cggaacgttc tcgaaagcgc tggatctgac tgatttgtgg caacttttta
     6721 tcccgttgct gatagccatc ccgctggtga tgggcttaag catcctgctg ctgaaaaaac
     6781 aggagggatg atgcgccatt tacgcaatat ttttaatctg ggtatcaaag agttgcgcag
     6841 tctgctcggt gataaagcga tgctgacgct gattgtcttc tcgtttacgg tgtcggtgta
     6901 ttcgtcagcg accgttacgc caggatcgtt gaacctcgcg ccgatcgcca ttgccgatat
     6961 ggatcaatcg cagttatcga accggatcgt taacagcttc tatcgtccgt ggtttttgcc
     7021 accggagatg atcaccgccg atgagatgga tgccggactg gacgccggac gctatacctt
     7081 cgcgataaat attccgccta attttcagcg tgatgtcctc gccggacgcc agccggatat
     7141 tcaggtgaac gtcgatgcca cgcgcatgag ccaggcattt accggcaatg ggtatatcca
     7201 gaatattatc aacggtgaag tgaacagctt tgtcgcgcgc taccgtgata acagcgaacc
     7261 gttggtatcg ctggaaaccc ggatgcgctt taacccgaac ctcgatcccg cgtggtttgg
     7321 cggggtgatg gcgatcatca acaacattac catgctggcg attgtattga ccggatcggc
     7381 gctgatccgc gagcgtgaac acggcacggt ggaacactta ctggtgatgc cgataacgcc
     7441 gtttgagatc atgatggcga agatctggtc gatggggctg gtggtgctgg tggtatcggg
     7501 attatcgctg gtgctgatgg cgaaaggtgt actgggcgta ccgattgaag gctcgatccc
     7561 gctgtttatg ctgggcgtgg cgctcagtct gtttgccacc acgtcaatcg gcatttttat
     7621 ggggacgata gcgcgttcaa tgccgcaact ggggctgctg gtgattctgg tgctgctgcc
     7681 gctgcaaatg ctttccggtg gatccacgcc gcgcgaaagt atgccgcaga tggtgcagga
     7741 cattatgctg accatgccga cgacacactt tgttagcctc gcgcaggcca ttctctaccg
     7801 gggtgccgga ttcgaaatcg tctggccgca gttcctgacg ctgatggcaa ttggcggcgc
     7861 gtttttcacc attgcgctgc tgcgattcag gaagacgatt gggacaatgg cgtaaggaag
     7921 ggaaacttga cttcatactg tgatgtcata ctattgatgt cataaagtga agtcataagg
     7981 acatgccatg agaaccaagg tacaggcttt acggaagaaa aaaaatacat tggatcaaat
     8041 atttaaaact cctgttcctc aagggatcaa gtggtctgat atagagtcac tggttaaagc
     8101 attaggcgga gaaattaagg aaggaagagg ttcgcgttgt aagttcatac taaatatgag
     8161 cgttgcgtgt ttccatcggc ctcatccgtc gccagatacc gataaaggcg ctgtagaaag
     8221 cgtgcgtgac tggttactaa gtataggggt aaaaccatga tcaaactgaa gacgccaaac
     8281 tctatggaga ttgccgggca accagctgtt attacctacg tgccagaact gaatgcattt
     8341 cgtggcaaat tcctgggctt gtcaggttat tgtgattttg tctctgacag cattcagggg
     8401 ctgcaaaaag aaggggaact ttccctgcga gagtatcttg aggattgtaa agctgcgggt
     8461 attgagccgt atgcgcgtac agaaaagatt aagaccttta cgttacgcta tccagaatcg
     8521 ctgagtgaac gccttaataa tgcggctgcg cagcaacaag tctctgttaa tacctatatt
     8581 attgaaacac tgaatgagcg tttgaatcat ttataaattt tagcggcgta cattacgca
//
LOCUS       DAZOZY010000167         8633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZOZY010000167 DAZOZY010000000
VERSION     DAZOZY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.168"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHURQ_003303"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHURQ_003303"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR0825019.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHURQ_003304"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHURQ_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR0825020.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHURQ_003305"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHURQ_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR0825021.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHURQ_003306"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHURQ_003306"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR0825022.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHURQ_003307"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHURQ_003307"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR0825023.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHURQ_003308"
     CDS             complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHURQ_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR0825024.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHURQ_003309"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHURQ_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR0825025.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2247 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa tagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taa
//
LOCUS       DAZOZY010000168         8611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZOZY010000168 DAZOZY010000000
VERSION     DAZOZY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.169"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHURQ_003310"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHURQ_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR0825026.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHURQ_003311"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHURQ_003311"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR0825027.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHURQ_003312"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHURQ_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHURQ_003313"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHURQ_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR0825028.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHURQ_003314"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHURQ_003314"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR0825029.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHURQ_003315"
     CDS             complement(6490..6846)
                     /locus_tag="ACHURQ_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR0825030.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHURQ_003316"
     CDS             complement(6850..7266)
                     /locus_tag="ACHURQ_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR0825031.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHURQ_003317"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHURQ_003317"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR0825032.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         2180 a         2148 c         2105 g         2178 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgccaaag atttttctta t
//
LOCUS       DAZOZY010000169         8596 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZOZY010000169 DAZOZY010000000
VERSION     DAZOZY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.170"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(175..1839)
                     /gene="glnS"
                     /locus_tag="ACHURQ_003318"
     CDS             complement(175..1839)
                     /gene="glnS"
                     /locus_tag="ACHURQ_003318"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR0825033.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2098..4044)
                     /gene="nagE"
                     /locus_tag="ACHURQ_003319"
     CDS             complement(2098..4044)
                     /gene="nagE"
                     /locus_tag="ACHURQ_003319"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR0825034.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4377..5177
                     /gene="nagB"
                     /locus_tag="ACHURQ_003320"
     CDS             4377..5177
                     /gene="nagB"
                     /locus_tag="ACHURQ_003320"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0825035.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5237..6385
                     /gene="nagA"
                     /locus_tag="ACHURQ_003321"
     CDS             5237..6385
                     /gene="nagA"
                     /locus_tag="ACHURQ_003321"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR0825036.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6394..7614
                     /gene="nagC"
                     /locus_tag="ACHURQ_003322"
                     /gene_synonym="nagR"
     CDS             6394..7614
                     /gene="nagC"
                     /locus_tag="ACHURQ_003322"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR0825037.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7662..8414
                     /gene="nagD"
                     /locus_tag="ACHURQ_003323"
     CDS             7662..8414
                     /gene="nagD"
                     /locus_tag="ACHURQ_003323"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR0825038.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8465..8596)
                     /locus_tag="ACHURQ_003324"
                     /pseudo
     CDS             complement(8465..8596)
                     /locus_tag="ACHURQ_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2101 a         2268 c         2193 g         2034 t
ORIGIN      
        1 ggatgcggcg tgaacgcctt aaccggccta caaaagcacg caaactcaat atattgcaga
       61 gatcatgtag gcctgataag cgtagtgcat caggcaattt agcgtttgaa actgtttgtc
      121 tgttccttgc cggatgcgac gtaaacgccc catccggcat agcgaaactt aaaattactc
      181 gcctactttc gcccaggtat cacgcagccc aacggtgcgg ttaaataccg gtttttccgc
      241 cgtagaatgg cggctatcga ggcagaagta accttcacgc tcaaactgga atgctttacc
      301 cgcaaccgca tctttcagcg acggttcagc aaagccctgt ttgatcacca gcgattccgg
      361 gttaatcacc gacaggaaat catccgcagc accagggttc ggcacgctga acagacggtc
      421 gtacagacgg atttcaaccg gcagcgcatg tgccgcgctc acccagtgaa taacaccttt
      481 gactttacga ccatctgccg gatctttgct taaggtgtcg gcgtcataag tacagaagat
      541 ggtggtgata ttgccttcgg catctttctc cacgcgttct gccttaatga cgtaagcatt
      601 acgcagacgc acttctttac ccagcaccag acgtttgtac tgcttgttag cttcttcgcg
      661 gaaatcggcg cgatcgatcc aaatctcacc gctaaacggc acctggcggc tacccatttc
      721 cggtttgttc ggatggttcg gcatggtaac catttcgcct tcgccctgat agttttcgat
      781 aaccagtttc accggatcga taaccgccat tgcgcgcggg gcgttttcgt tgagatcttc
      841 acggatgcag gattccagcg acgccatctc aatggtgttg tcctgcttgg tcacgccgat
      901 gcgtttgcag aactcacgaa tagaagcagc agtgtaacca cgacgacgca gaccggaaat
      961 ggtcggcata cgcgggtcat cccagccttc aacgtgcttg tcggtcacca gcaggttcag
     1021 cttacgcttg gacatcacag tgtattccag attcaggcgc gagaattcgt actggcgcgg
     1081 gtgaacagga atggtgatgt tgtccagcac ccagtcgtac aggcgacggt tgtcctggaa
     1141 ctcaagcgta cacagagagt gcgtaatacc ttccagggca tcgctgatgc agtgggtgaa
     1201 gtcgtacatc gggtagatgc accacttgtt gccagtctgg tggtgttcag caaatttaat
     1261 gcggtacagc accggatcgc gcatcacgat aaacggtgaa gccatgtcga ttttcgcacg
     1321 caggcaggct ttaccttctt caaaaccacc ggcacgcatt ttttcgaaca gcgccaggtt
     1381 ctcttcaacg ctgcggtcgc ggtacgggct gtttttaccc ggttgcgtca gggtgccgcg
     1441 gtattcgcgg atctgttccg gcgtcagttc atcaacgtac gccaggcctt tattgatcag
     1501 ttcgatcgca taggcgtgga gctgatcaaa ataatcggag gagtaacgga cgttaccaga
     1561 ccagtgaaaa cctaaccact ctacgtcgtt tttgatcgac tcaacatact cgatatcttc
     1621 ttttaccggg ttagtgtcgt cgaaacgcag gttgcactgg cctttatagt cctgggcgat
     1681 cccgaagttc aggcagatag atttcgcatg gccaatatgc agatagccat tcggctccgg
     1741 cgggaaacgg gtgtgtactg tggtgtgctt accactggcc agatcttcat cgatgatctg
     1801 acggataaag ttagtcgggc gggcttctgc ctcactcatc gtggattcct caaagcgtaa
     1861 acaacgtata acggcgtatg atcttataag cgggacaggc tgacaactgt tagttagcga
     1921 aaatcgaaaa tctcactgag attatgggga tgattgatga aaaaaaaagc ggcagagaag
     1981 atctccaccg cttttgccag tcccgcgtca ttgttggatg cgcgtcttat ccaaccagga
     2041 ctgagctttt gtaggcctga taagacgcgt caagcgtcgc atcaggcata aagcagatta
     2101 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca ttgccctgag ctttaatgat
     2161 caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg aacgggcgtt
     2221 agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctca cctgcgcgcc
     2281 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatgtg
     2341 gacgacaatc tccgcgcctt tttcggtttc taggcagaac gcgtggttgg tgttgaagat
     2401 tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2461 accgtcaccc accgctttgc tggcgaatgc ttcgtcagaa acctgatcca gtgccacgac
     2521 atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg
     2581 ttttgctaca ggagcggcag ttgccggagt tgcttcagct gacgcagctg ctaccgggcc
     2641 acgggcaacg actttcttca tcgcatcgcc aatggattct gctttcgcgc caacaatcac
     2701 ctgaatggtc tgtttgttca gttttactac accagaagca cccagacgtt tacacatcgc
     2761 atcgttgacg cgagctgagt cagccacggt aaggcgcaga cgggtaatac aggcgtcaat
     2821 cgctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgagtcag
     2881 accttcttca gtattgctgt tagcctcttc agttacgatc tcgtcttctt tatcttcacg
     2941 acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     3001 gaagaagaca acacccatca ccagcagcat ccagacgttc tggctggcgg ccggcaggtt
     3061 atacatcaac gcgtagtcga tagcccccgc agagaaagag aagccagcat ggatacccag
     3121 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3181 agcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3241 agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta
     3301 catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa
     3361 catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc
     3421 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag
     3481 accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcctgc
     3541 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt actggcggcc agacgtaacc
     3601 aaaaatggcc gccagcacca ggcagaagaa gccggtggca atcggcacaa agcgtttgcc
     3661 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc
     3721 accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3781 caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagcgccg ccgcacctgc
     3841 gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa
     3901 aatcgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3961 cagcaacagt gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg
     4021 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     4081 aaagccgtat ttttattttg ctgcaaattg tactgccgat gttctgtaat cagattgtta
     4141 gatcatctgc tacagagtgt gtgaaaattt aattcgtatc gcaaattaaa cgcgtgcctt
     4201 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaagcagaaa
     4261 acttatttta tcattcaaaa aatcaggtcg gattgacgcc tgtctgcgca aatccaggtt
     4321 acgcttaaag atgcctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4381 gactgatccc cctaactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4441 atcgtatcaa tgcgttcaaa ccgactgccg atcgtccgtt tgttctgggc ctgccgactg
     4501 gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4561 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4621 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4681 aaaacatcaa ccttctcaac ggcaacgccc cggatatcga cgccgaatgc cgccagtatg
     4741 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4801 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4861 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc
     4921 caaaatatgc cctgactgtc ggtgttggta cattgctgga tgccgaagaa gtgatgattc
     4981 tggtgctggg tagccagaaa gcactggcac tgcaggctgc agttgaaggt tgcgtgaacc
     5041 acatgtggac catcagctgt ctgcaactgc atccaaaagc gatcatggtg tgcgatgaac
     5101 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5161 atatcaaagg tctgtaattg ttatccctgc cctggctcct tgctcagggc aatatttttt
     5221 aaaatcgggg ggcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5281 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5341 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5401 tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt
     5461 cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5521 tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg
     5581 cgagtacctg gcaagacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct
     5641 gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5701 ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat
     5761 ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca
     5821 ctccaacgcg acgttgaaag aagcgaaagc cggtttccgc gcggggatta cctttgccac
     5881 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5941 cctcgacgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc
     6001 caacattcgt aacgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac
     6061 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6121 taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6181 aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc
     6241 gacgctctat ccggcgcgtg cgattggcgt tgagaaacgt ctcggcacac tcgccgcagg
     6301 taaagtagcc aacctgacct cattcacacc tgattttaaa atcaccaaga ccatcgttaa
     6361 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6421 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6481 tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta
     6541 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6601 tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc
     6661 ggcgtacggc ttggtcgtca cgacgccacc atcaccctgt ttgatctcag cagcaaagtg
     6721 ctggcagaag aacattaccc gctgccggaa cgtacccagc aaacgctgga acatgccctg
     6781 ttgaatgcca ttgctcagtt tattgatagc taccagcgca aactgcgcga gctgatcgcg
     6841 atttcggtga tcctgccagg gcttgttgac ccggacagcg gcaaaattca ttacatgccg
     6901 catattcagg tagaaaactg ggggctggta gaagctctgg aagaacgttt taaagtgacc
     6961 tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt
     7021 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     7081 tctaacgggc gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag
     7141 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc
     7201 gccaacgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgc
     7261 gtgccgctgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagt
     7321 ctggcgtcgg aagtaattga gtatgtcggt cgtcatctgg gtaaaaccat cgccattgct
     7381 atcaacttat ttaatccgca aaaaattgtt attgccggtg aaatcaccga agccgataaa
     7441 gtgctgctcc ctgctattga aagctgcatt aatacccagg cgctgaaggc gtttcgtact
     7501 aatctgccgg tggtacgttc tgagctggat caccgctcgg caatcggcgc ttttgcgctg
     7561 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7621 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7681 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7741 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7801 agaccgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg
     7861 tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgccaggaa ggcaagaaag
     7921 catatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7981 ccgatgtgaa ccctgatttc gtgattgttg gcgaaacgcg ttcctacaac tgggacatga
     8041 tgcataaagc ggcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca
     8101 cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct
     8161 ccgggcgcaa accgttctat gttggtaagc ccagcccgtg gatcatccgc gcagcattaa
     8221 acaaaatgca ggcgcattcg gaagaaacgg tgattgtcgg cgataacttg cgcaccgaca
     8281 ttctggcggg cttccaggca ggtctggaaa cgattctggt gctttctggt gtttcatcgc
     8341 tcgacgatat cgacagtatg ccgttccgcc caagctggat ttacccgtca gtcgctgaaa
     8401 tcgacgttat ctgaatacca cccacgtctg cggacgtggg tggctctgat gacaaacgcc
     8461 aaactgcctg atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt
     8521 catgcttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt
     8581 tatcagcaat ttggcc
//
LOCUS       DAZOZY010000170         8545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZOZY010000170 DAZOZY010000000
VERSION     DAZOZY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.171"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            94..1737
                     /gene="eptA"
                     /locus_tag="ACHURQ_003325"
     CDS             94..1737
                     /gene="eptA"
                     /locus_tag="ACHURQ_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR0825039.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            1734..2402
                     /gene="basR"
                     /locus_tag="ACHURQ_003326"
     CDS             1734..2402
                     /gene="basR"
                     /locus_tag="ACHURQ_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR0825040.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2403..3503
                     /gene="pmrB"
                     /locus_tag="ACHURQ_003327"
     CDS             2403..3503
                     /gene="pmrB"
                     /locus_tag="ACHURQ_003327"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR0825041.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(3479..3568)
                     /gene="pmrR"
                     /locus_tag="ACHURQ_003328"
     CDS             complement(3479..3568)
                     /gene="pmrR"
                     /locus_tag="ACHURQ_003328"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR0825042.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(3680..5182)
                     /gene="proP"
                     /locus_tag="ACHURQ_003329"
     CDS             complement(3680..5182)
                     /gene="proP"
                     /locus_tag="ACHURQ_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR0825043.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5446..6312)
                     /locus_tag="ACHURQ_003330"
     CDS             complement(5446..6312)
                     /locus_tag="ACHURQ_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR0825044.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            complement(6309..8537)
                     /gene="crfC"
                     /locus_tag="ACHURQ_003331"
     CDS             complement(6309..8537)
                     /gene="crfC"
                     /locus_tag="ACHURQ_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR0825045.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2178 a         2291 c         2113 g         1963 t
ORIGIN      
        1 tccagcaaac ataaaagcca accttaagaa cttaaggttg gcttaatttt gctttgcgag
       61 catatgcgca ctttgttcga tggaaacacc gtgatgttga agcgcctact aaaaagcccc
      121 tctttgaatt tactcgcctg gctattgttg gccgcttttt atatctctat ctgcctgaat
      181 attgcctttt ttaaacaggt gttgcaggcg ctgccgctgg attcgctgca taacgtactg
      241 gttttcttgt cgatgccggt cgtcgctttc agcgtgatta atattgtcct gacactaagc
      301 tctttcttat ggcttaatcg accactggcc tgcctgttta ttctggttgg cgcggctgca
      361 caatatttca taatgactta cggcatcgtc atcgaccgct cgatgattgc caatattatt
      421 gataccactc cagcagaaag ttatgcgctg atgacaccgc aaatgttatt aacgctggga
      481 ttcagcggcg tgcttgctgc gctgattgcc tgctggctaa aaatcaaacc tgccacctcg
      541 cgtctgcgca gtgttctttt ccgtggagcc aatattctgg tttctgtact gctgattttg
      601 ctggtcgccg cactgtttta taaagactac gcctcgttgt tccgcaataa caaagagctg
      661 gtgaaatcct taagcccctc taacagcatt gttgccagct ggtcatggta ctcccatcag
      721 cgactggcaa atctaccgct ggtgcgaatt ggtgaagacg cccaccgcaa cccgttaatg
      781 cagaacgaaa aacgtaaaaa tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag
      841 aacttctccc tcaacggcta cccgcgtgaa actaacccgc ggctggcgaa agataacgtg
      901 gtctatttcc ctaataccgc atcttgcggc acggcaacgg cagtttcagt accgtgcatg
      961 ttctcggata tgccgcgtga gcattacaaa gaagagctgg cacagcacca ggaaggcgtg
     1021 ctggatatca ttcagcaagc gggcatcaac gtgctgtgga atgacaacga tggcggctgt
     1081 aaaggtgcct gcgaccgcgt gcctcaccag aacgtcaccg cgctgaatct gcctggtcag
     1141 tgcatcaacg gcgaatgcta tgacgaagtg ctgttccacg ggctggaaga gtacatcaat
     1201 aacctgcagg gtgatggcgt gattgtctta cacaccatcg gcagccacgg tccgacctat
     1261 tacaaccgct atccgccgca gttcaggaaa tttaccccaa cctgcgacac caatgagatc
     1321 cagacctgta ccaaagagca actggtgaac acttacgaca acacgctggt ttacgtcgac
     1381 tatattgttg ataaagcgat taatctgctg aaagaacatc aggataaatt taccaccagc
     1441 ctggtttatc tttctgacca cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt
     1501 ctgccttatg ccatcgcccc ggatagccaa aaacaggtgc cgatgctgct gtggctgtcg
     1561 gaggattatc aaaaacggta tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg
     1621 caacactatt cacaagacaa tttattctcc acgctattgg gattaactgg cgttgagacg
     1681 aagtattacc aggctgcgga tgatgttctg caaacttgca ggagagtgag tgaatgaaaa
     1741 ttctgattgt tgaagacgat acgctgttat tgcagggact gattctggcg gcgcaaaccg
     1801 aaggctacac gtgcgatggc gtaacaaccg cgcggatggc ggaacaaagc cttgaggccg
     1861 gtcattacag cctggtggta ctggatttag ggttacccga cgaagatgga ctgcattttc
     1921 tcgcccgtat ccggcagaaa aaatacaccc tgccggtact gatcctcacc gctcgcgata
     1981 cgctgaccga caaaatcgcc gggctggatg tcggtgccga tgactatctg gtgaagcctt
     2041 ttgcgctgga agagttacac gcccgtatcc gcgccctgct acgacgccat aataatcagg
     2101 gcgaaagtga gctgattgtt ggcaatctga cgctgaatat gggtcgccgt caggtatgga
     2161 tgggcggtga agagttgatt ctgacgccca aagaatatgc tctgctgtca cggttaatgc
     2221 tcaaagcagg cagtccggta catcgggaaa ttctctacaa cgacatctat aactgggaca
     2281 atgaaccctc gaccaacacc ctggaagtgc atatccacaa tctgcgcgac aaagtgggca
     2341 aagcccgtat ccgcaccgtg cgcggctttg gctatatgct ggtcgcgaat gaggaaaact
     2401 aattgaatct gatgcgtttt ctgcgccgac caatatcgct gcgccaacgg ctgatattga
     2461 ccatcggggc cattttgttg gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa
     2521 gtaccgagca gattcagctg tttgagcagg cgctacgcga caatcgcaac aacgatcgcc
     2581 acatcatgcg tgagatccgc gaggcggtcg ccagcctgat tgtccccggc gtctttatgg
     2641 tcagcctgac gctatttatc tgctatcagg cggtacgccg catcacccgc ccgctggcgg
     2701 agctgcaaaa agagctggaa gcgcgcaccg ccgacaactt aacgcccatt gccattcaca
     2761 gcgccaccct cgaaatcgaa gcggtggttt cggcgttgaa cgatctggtc agtcgcctga
     2821 cgagcacgct ggataacgaa aggttattta ccgctgacgt cgcgcacgaa ctgcgaacgc
     2881 cactggcggg ggtgcgtttg catctggaac tgctggcgaa aacgcatcac attgatgtag
     2941 caccgttagt ggcacggctt gatcagatga tggagagcgt ctcccagctg ctgcaactgg
     3001 cgcgtgccgg acagtcattt tcttccggta attatcaaca tgtaaaactg ctggaagatg
     3061 tcattctccc ctcgtatgac gaactcagta ccatgctcga ccagcgacag caaaccctgc
     3121 tattgccaga gagcgccgcc gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt
     3181 tattgcgaaa cctggtagaa aacgcccatc gttacagccc acaaggcagc aacattatga
     3241 ttaagctgca agaagatggc ggagcggtca tggccgttga agatgaagga ccgggtattg
     3301 atgagagtaa atgcggggag ttgagtaaag cgtttgtacg tatggacagc cgttatggcg
     3361 ggattggtct ggggttaagt attgtcagcc gcattacaca gttgcatcac gggcagtttt
     3421 tcctgcaaaa ccggcaagag acttccggca cgcgggcctg ggtacggctg aagaaagatc
     3481 agtacgtagc aaaccagata taaagaaagc tgctgaccac cagcacactg aacacggtag
     3541 ttaaactttc ataaacacgg tttttcattg cactctcctc cgttaacctg gaggagagta
     3601 tgcgcgtcag agattaagtg aaccttaaga gttcaattgg cctgataaga cagcgtcaca
     3661 tcaggccatc cgtttcagct tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac
     3721 gtttcgcctg caaatcggca atctcgtggt caatatcatc gattttctgc tcgatattat
     3781 cgtaatgctc gacgagaatt tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac
     3841 ctttcaacgg acgatttgcc gtctctttca tggttacgcc ggtgattaaa ccaatcaccg
     3901 ccactaccat caggtaatag gcaggcatca tcagattctg cgagctttcg accagccagg
     3961 ccgccagtgt tggcgtcaga ccggcaacca gcaccgaaat attaaatgcc gccgccagcg
     4021 cgctgtaacg gatatgcgtc gggaacatcg ctggcaaggt agaagccata acgcccgtaa
     4081 agcagttaag gatcaccgcc agcatcagta acccggcaaa aatcaggccg atgacgttac
     4141 tgttaatcag aataaacgcc gggatcgcca acacaaacag ggcaacacta ccaagtagca
     4201 caaacggacg acggccaaaa cggtcactca gcaagcccat caccggctgg acaaacagca
     4261 taccgatcat aatggcgata ataatcagca ccccgtggtc ttcggagtaa tgcaggttat
     4321 gcgacaaata actcggcata taggtcagca acatgtagta agtcacgttg gtggcaatta
     4381 ccagaccaat acatgtcaac aggctgcgcc agtatttagt ggcaatctct ttaaacgaga
     4441 ctttcgggcc atcctgcaaa ccttcgcggt cgccctgttc cagtttatca acatgctgct
     4501 ggaacgccgg agtttcttcc agcgcatggc gcaggtaaag cccgataatc cctaacggca
     4561 gagcaataaa gaacggaata cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg
     4621 tcgaaattaa caccaccacg cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca
     4681 gccagctgcc cataaagcca cgtttacggt caggggagta ttccgcaaca aatatcgacg
     4741 ccccggtata ttcaccgccg accgagaaac cttgtgccat cttacagatc aacagcagaa
     4801 tcggtgccca aataccaatc gtgtcgtagg acggtattaa gccaatacag aacgtactga
     4861 tcgacataat cacaatagtg atagcgagga tcttctggcg accatattta tcgcccaaca
     4921 taccaaagaa gagcccgcca agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg
     4981 cagcaaccat ctgcacgctg gggtcagccc ccgggaaaaa aactttacct aatgcgtaag
     5041 caacaaaacc ataaacacca aaatcgaacc attccattgc attacccagt gatgctgcgg
     5101 taatggcttt acgcagttta ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt
     5161 ttactttttt ccttttcagc atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc
     5221 agaaaaatca gcgcgacggc cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta
     5281 acaagcgtag cgggtttgct tacaccctcc ggttaaggaa tgtacaatca aaccagttga
     5341 cgcctgaatt gggcagttaa tgaccttttt accctaccag tgtttaaatt tgtgatcaac
     5401 ttcacacatg gatttcgttt aggactcatt gatgtaactg attatttacg ccaaatttac
     5461 agagaagatt tcctcgacca ttgcccccac caaacgctgc gctgaacaga gtcgtggcat
     5521 ccccagcgcg actgtttgcc agcgttgggt cactgaccag caaacgggct gtttatctgc
     5581 cgtggaccac tttcccagcc aacttaaaaa atcagcatgc tcccctcccg aagaggcaaa
     5641 ccactgatgg taatagtgac gcgtggtcgg tgccgcattg atactctctg ccagatgttg
     5701 tgtcgctttc tcgcgcaaat cacccagtaa caacaccacc gcttgcgggc ttcccagacg
     5761 gcaggcatcg ccaaatgcac cccagcgcaa ctcctccaga acgataaaac agcgcccagg
     5821 caacgaccag gcgttatacg ccccagcacg ccagcgggta aaaatttgct cgctctgatc
     5881 acctgcctgc accagtaggc ctcgttgcgt taacgccttt tccttccagc gacaacgggc
     5941 tgaaaaccac tcatacaagg cggcaatttg ctgggggagc tccgggaaag cagagtgctg
     6001 gcggtgatcc attttttgca gcagcgccag atgctgcgcg atctgcgtca gcgccagatg
     6061 accgggatcc agcatcgacg ccagtttttc caccattgtc agtcgcaggc taagcggcgc
     6121 atttaacatg ccgccgttcg tccatgtctg aagctgcaca cgcgaaagaa gttcctgcat
     6181 cagtcgttca cgaaactgct gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc
     6241 cagatccact aaaaagcggg gataaaccga ctccagcacg cgaccggggc cgtccagtaa
     6301 cgtcttggtc aatatcgttc tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg
     6361 gcgtcggtgt aaatccagcc gtgagtcatc acagtctgct gcaattgctg ctgcatcagc
     6421 ctgaccaccg attcattttg ttgacgcaga gccaggcttt cgcgtaaacg cgtctgtaat
     6481 tccgtcaaac atgaagcgaa ctcagcgcaa aaagtattca tgcctgccgt aacagattca
     6541 tcgacctgct ctgccagaac tttagccatt tgttggcaat aaagatcgac ttctgcgctt
     6601 aaagctcgtt gcaacacact gtaatcaacc gtctctgtcg gggatttctc atttccccgt
     6661 ccccagtcgg gctgattcaa ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg
     6721 ctctgctcgt ccgttgcatc ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg
     6781 acgtggggaa ctactccgtg aaaaacagga aaatgaaacc caggacgaaa ccctgactcg
     6841 ctcaattcca ttttgacttg ttgctcaatg ggccgaataa catcggttaa cacccggcaa
     6901 agggtggatt caagctcggc aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc
     6961 gtctgtaaca gtgtctcaca gcaggtacga atctcgctta acggctccga atcatcctga
     7021 aacaaggcgg ctaactgcgc attcagcgca tcttgctgtt gacgcagaaa gtggttggcg
     7081 gaggtcaggg ccagctcgat ttcatgttta atctcgccgc tcacctgcgc ctgattgagt
     7141 tgcaatagct gcaaactttc ttcgacctga tggatatttt gccgcaattg ttcacaagcg
     7201 acgtttaacc cgtgcgcacg aaaatccagg tattcccgcg cctgctgcgc gtaattcaac
     7261 agtttatgcg cagcagatcg cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc
     7321 aacgcctgaa ttggctgggc gaacagcgaa tcttcccaca actgatcggc agcatgacga
     7381 atatgttcga ggtccgccag atcggcatga cgccagcgcc tgccgagcgc ggcatgggca
     7441 aaatcttcca cccagcgttg ttgctctggc gctggtaact taccgttgtt ggctaactca
     7501 tggcgcgctc gatttgccaa gtagccccac atcgacgaca ccggaaatat ctgctgtggc
     7561 gtaatacagc ctttcatcag cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca
     7621 ctgttacgat cctgttgatc gaacttattg accagcacat acagtggcac cgattgcccc
     7681 accgccaaaa tcgcctcacg gacctcttca tcggagatcg atttcagttg cgtataatcc
     7741 agcaccgcca gtaccgccga ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc
     7801 ggttgcccgg cttcatttgg cccgggggta tccagtaacg tcaactgacc gggataactc
     7861 tccagccccg ccagatggac aaactccact tcaatcacgg gaatatgctc aatggcggcg
     7921 taagcagaaa aaggaaaatc gacgtccagc gccttcgcca gtcgcactaa atcattcaaa
     7981 cttttcagac aatgaaaaat aggctgggca cccagataat atttttcgaa agcgacgcca
     8041 ttttcgatcc gctgcataag cgcacgcata tctttatcta tttccagcac atcggtcaga
     8101 tgcttaatat cgcaatcacg caggcgctgt tgtaattgtt gaattaaaca atcgattggc
     8161 gcgacatgtg aaaaatgcag taccggttcc ttttgcccgg gcgtatggcg aataagcgtc
     8221 ggcagcgcag tcattgggcg attacgatta ggcagaacct ccgtaccaac aatggcatta
     8281 atggtggttg atttccctgc tttcatagta ccgacaatcg ccagcaccat ttccagtcgg
     8341 gaaattttac tcaactcatt attcagcatc gcgtgacgtt cggcgatatt aggctgactc
     8401 cagggtaaag ccagttgtgg cgcgtcgtct ccgggtacag agagaggcat tttttccagt
     8461 aactgcaact gttggcgaga aagctgtaac aggcgttcag cctcctgact taactcatac
     8521 agggtctgtg tgtacataga aaatt
//
LOCUS       DAZOZY010000171         8466 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZOZY010000171 DAZOZY010000000
VERSION     DAZOZY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.172"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(203..279)
                     /locus_tag="ACHURQ_003332"
     tRNA            complement(203..279)
                     /locus_tag="ACHURQ_003332"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asp,seq:gtc)
     gene            complement(412..1143)
                     /gene="dnaQ"
                     /locus_tag="ACHURQ_003333"
     CDS             complement(412..1143)
                     /gene="dnaQ"
                     /locus_tag="ACHURQ_003333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR0825046.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1208..1675
                     /gene="rnhA"
                     /locus_tag="ACHURQ_003334"
     CDS             1208..1675
                     /gene="rnhA"
                     /locus_tag="ACHURQ_003334"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR0825047.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1672..2394)
                     /locus_tag="ACHURQ_003335"
     CDS             complement(1672..2394)
                     /locus_tag="ACHURQ_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0825048.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2428..3183
                     /gene="gloB"
                     /locus_tag="ACHURQ_003336"
     CDS             2428..3183
                     /gene="gloB"
                     /locus_tag="ACHURQ_003336"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR0825049.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3255..4613
                     /gene="mltD"
                     /locus_tag="ACHURQ_003337"
     CDS             3255..4613
                     /gene="mltD"
                     /locus_tag="ACHURQ_003337"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR0825050.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4662..5285)
                     /locus_tag="ACHURQ_003338"
     CDS             complement(4662..5285)
                     /locus_tag="ACHURQ_003338"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR0825051.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5289..6089)
                     /locus_tag="ACHURQ_003339"
     CDS             complement(5289..6089)
                     /locus_tag="ACHURQ_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR0825052.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6330..7244
                     /gene="yafC"
                     /locus_tag="ACHURQ_003340"
     CDS             6330..7244
                     /gene="yafC"
                     /locus_tag="ACHURQ_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR0825053.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7241..8044)
                     /gene="dkgB"
                     /locus_tag="ACHURQ_003341"
     CDS             complement(7241..8044)
                     /gene="dkgB"
                     /locus_tag="ACHURQ_003341"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR0825054.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8207..8283)
                     /locus_tag="ACHURQ_003342"
     tRNA            complement(8207..8283)
                     /locus_tag="ACHURQ_003342"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8247..8249),aa:Asp,seq:gtc)
BASE COUNT         2273 a         2029 c         2035 g         2129 t
ORIGIN      
        1 atgaaaatca taacattcaa atatatttaa attgaatgat tcgatagtat gattaaatat
       61 taatgatatg gtctaccata tgtgattaac acatttacag cataacaaaa ccatagaccg
      121 aagaaatagc gggatttaaa tgcaaaaaaa ccgcaaaatt aaaaatcttg cggttctctg
      181 aactcatttt catgagtgaa tttggcggaa cggacgggac tcgaacccgc gcccccctgc
      241 gtgacaggca ggtattctaa ccgactgaac taccgctccg cgttgtgttc cgttgggaac
      301 gaggcgaata ttacggattg cctcaccctt cgtcaacggt ttttctcatc ttttgaatcg
      361 tttgctgcaa aaatcgccca agccgctatt tttagcgcct tttacaggta tttatgcccg
      421 ccagaggcag cttccgccct tcttctccac cagatcaaga cgggcttcat gagctgcaag
      481 ctcttcatct gtcgcaaaaa caacgcgtaa cttacttgcc tgacgtacaa tgcgctgaat
      541 tgttgcttca ccttgttgct gctgtgtctc tccttccatc gcaaaagcca tcgacgtttg
      601 accaccggtc atcgccagat aaacttccgc aaggatctgt gcatcgagta atgccccgtg
      661 tagcgttcgt ttactgttat ctatttcgta gcgggcacat aacgcatcga ggctgttgcg
      721 cttaccagga aacattttcc tcgccaccgc aaggctatcg gtgaccttac agaaggtatt
      781 ggtcttcgga atatcgcgct taagcaacga aaactcgtag tccataaagc cgatatcgaa
      841 cgctgcgtta tggatcacca actccgcgcc gcggatatag tccatgaact catcggctac
      901 ttcggcaaac gtgggcttat cgagcaaaaa ttcatcggca ataccatgta cgccaaaggc
      961 ttccggatcc accagccgat cgggtttgag ataaacatgg aagttattgc ccgtcaggcg
     1021 acggttcacc acttcaacgg caccaatctc aatgatcttg tggccttcat agtgcgcacc
     1081 aatctggttc ataccggtgg tttcggtatc gagaacgatc tggcgtgtaa ttgcagtgct
     1141 catagcggtc atttatgtca gacttatcgt tttacagttc gattcaatta caggaagtct
     1201 accagagatg cttaaacagg tagaaatttt caccgatggt tcgtgtctgg gcaatccagg
     1261 acctgggggt tacggcgcta ttttacgcta tcgcggacgc gagaaaacct ttagcgcagg
     1321 ctacacccgc accaccaaca accgtatgga gttgatggcc gctattgtcg cgctggaggc
     1381 gttaaaagaa cattgcgaag tcattttgag taccgacagc cagtatgtcc gccagggtat
     1441 cacccagtgg atccataact ggaaaaaacg tggctggaaa accgcagaca aaaaaccagt
     1501 aaaaaatgtc gatctctggc aacgtcttga tgctgcccta gggcaacatc aaatcaaatg
     1561 ggaatgggtt aaaggccatg ccggacaccc ggaaaacgaa cgctgtgatg aactggctcg
     1621 tgctgcggca atgaatccca cactggaaga tacgggctac caagtcgaag tttaagcttg
     1681 tggtttacga cattgccggg tggctccaac cgcctggcga attcgtggct tgtttttact
     1741 ctgtttcatc gggtttagcg ttaaaggaat agtccgtttc cgggcaacaa taagttgtaa
     1801 gcaaccaagc gcaggaatat gcgcattcaa tagttttcct ccgtgtttgt tccacgggag
     1861 aacgtggaaa cggctggcgt gcagcacttc aaaattcaac aaagagagcc agtccagttg
     1921 ccgcatcaga gtaaacatcc ggctgttata gggcgaggtt tttcgcaata ccggcacaag
     1981 tttgcgtaat cccattaaac taatgggatt gaagccacta atcaccagcc agccatcatc
     2041 aatcaatacc cgatcggttt cacgcaataa acgatgcgga tcggtgcacc acggcaatgt
     2101 atgtgccagt agacaaacat caacggattt atcggcaaaa ggaagatgaa gtggatccgc
     2161 ctgcacctgg acgggcattc cttgcgcaga aacattcact tgatgagaaa ccgcgcacgc
     2221 ttcgcaattg atttctgcgc ttaaattgcc aatcttaagc agatgaaaac catacatttt
     2281 agtgaaccac gggttgagct ggcgctccag cgccttgcga taaagctctc cccagggcaa
     2341 atcgccccag cgatcaggag ccacgacagt ttgagggact cttgccggtt tcatcacaac
     2401 cttccgtttc acactaagag gtaatctatg aatcttaaca gtattcccgc ctttgatgac
     2461 aattacatct gggttttgaa tgatgaagca ggtcgctgcc tgattgtcga tcccggagac
     2521 gcagagccag tattaaacgc cattgccgcc aataactggc aaccggaggc catatttctc
     2581 acccaccatc atcacgatca cgttggcggc gtaaaagaac tggtggaaaa gtttccgcaa
     2641 attgtggtgt atggtccaca agagacacaa gataagggaa caacacaggt agtcaaagat
     2701 ggcgaaactg ccttcgtttt ggggcatgaa tttagtgtaa ttgccacacc gggtcacact
     2761 ttaggacata tctgttactt cagtaaacct tatctatttt gcggcgacac gctgttttct
     2821 ggtgggtgtg gtaggttgtt tgaagggaca gcatcacaaa tgtatcaatc acttaataag
     2881 ttaagtgcgt tacctgacga tacattggta tgttgtgctc atgaatatac gttatcaaat
     2941 atgaagtttg ctttgagtat tcttccgcac gatttgtcca taaatgatta ttatcgtaaa
     3001 gttaaggagt tacgggcaaa aaatcaaata acactacccg taattctgaa aaatgagcgg
     3061 caaattaatg tttttttaag aacggaagat attgatttaa ttaatgtaat taatgaagaa
     3121 acattattgc aacaacctga agagcgtttt gcatggttaa ggtcaaagaa agataggttc
     3181 tgataaaact ttcttgtcat cggctccgtt cgccgttatg atcggtcgtc ttttaagcaa
     3241 ctattgacac acacatgaag gcaaaagcga tattactcgc ctctgtcctg ctcgtgggtt
     3301 gccagagtac cggcaacgtt caacagcacg cacagagcct ttctgcagct ggtcaagggg
     3361 aagcagcaaa gtttacaagt caggcacgat ggatggacga tgggacgtct atcgcgccag
     3421 atggtgactt gtgggctttc attggcgacg agctaaagat gggaattccg gaaaatgacc
     3481 ggattcgcga acagaaacag aaatatttac gcaataagag ctatctccac gatgtaactt
     3541 tacgggcaga gccgtatatg tactggatag cagggcaagt taaaaaacgt aacatgccta
     3601 tggaactggt actactaccc atagtggaga gcgcttttga tcctcacgca acgtctggcg
     3661 ccaatgccgc aggcatctgg cagatcattc cgagcacggg gcgcaattat ggtttgaaac
     3721 agacccgcaa ttatgacgcg cgtcgcgatg ttgttgcttc aacaactgcc gcgctgaata
     3781 tgatgcagcg tctgaacaaa atgtttgacg gcgactggct tctgaccgta gcggcttata
     3841 acagcggcga aggtcgggtc atgaaggcaa ttaaaacgaa caaagcgcgt gggaaatcca
     3901 cggacttctg gtcgttaccg ttgccgcagg aaacgaagca gtacgtgcct aaaatgctgg
     3961 cattgagtga tattctcaaa aacagcaagc gttatggcgt acgtctgcca acgaccgatg
     4021 aaagccgtgc tctggcgcgt gtgcacctga gtagcccggt tgaaatggcg aaggttgcag
     4081 atatggcggg gatttccgtc agcaagctga agacattcaa cgctggcgtg aaaggctcca
     4141 cgctgggcgc aagtgggccg cagtacgtga tggtgccaaa gaagcatgca gatcaactgc
     4201 gtgaatctct cgcttcaggc gaaattgctg ctgtacagtc gacgctggtt gccgacaata
     4261 cgccgcttaa cagccgtgtt tacaccgtac gctctggcga cacgctttca agcatcgctt
     4321 cacgtctcgg cgtaagcacc aaagatttgc agcagtggaa caaactgcgc ggatctaagc
     4381 tgaagccagg ccaaagtttg acgattggtg caggcagtag cgcacagcgg ttggcaaaca
     4441 acagcgatag cattacgtat cgtgtgcgca aaggcgattc gctttcaagc attgctaaac
     4501 gccacggcgt gaacatcaaa gatgtgatgc gctggaacag cgacactgcg aatctgcaac
     4561 caggcgataa gctgacgttg tttgtgaaaa acaacaacat gccagattcc tgacaaacca
     4621 gataataaaa aaggcaccga ttcccccggt gcctttttta tttatgccgc tttatgtgct
     4681 tctaccatga tgatatcact ggtgaaagag ccatcagtct gcaaggaaaa atacgttctc
     4741 acctctgtcg atgcgctctg ttggtaaatg cgaatagcgt ctactaacgc ttctggcgta
     4801 cgcattctca cgacccatga agaaaattcc agcggtaact tatctgtaat taaattatca
     4861 actatcagat tggcttcatt gattaacgtc aaccacgcac cgctggcgta gtttcgtacg
     4921 tgagaggtat cgcgtaatgc ttctaccgcc tgtaaccaga tgtcgcgcac tgggtgaccc
     4981 ggagacatta catccatcac aatcagccta ccgccaggtt tcaatatcct attcacttct
     5041 cgcagtgctg caccaacatc atgccagtga tgggcagaat aacggctgat aacaatatca
     5101 aatgcgttat cggcaaatgg cagacttgcg gcatatccct ggcgggtggt gatatttttc
     5161 agttgccggg cttcggcagc ttgtgccacg acatccagca tgtgggcaga taagtcatac
     5221 gccaccaccg cgctcacgtt ttgcgcagcg ccaaacgttg gtctgccctg cggcagacct
     5281 gacatacctt atttatcagg cttgccggga ctgaattcaa cgagtagcgg attgtgatcg
     5341 gaagcgcgcg taaccagtac agaagcttca ctgacgttca gaccacggta gaaaacaaaa
     5401 tcgagcgggc gaccaaacgc ccgacggcgc tgatcatcgg taaaacgcac ctggcgcagc
     5461 gacatttccc gcgcaaagcg atataacgcg ttcatccttc tacggctcca ggcattgaaa
     5521 tctcccgcca taatgaccgg gccgctgtgg tgagctatct gatcaccaat aggaagtaac
     5581 tgcttactat agacatccac gcccagactg aagttgacgg cgtgtatatt aaccaccatc
     5641 aacaggcggg tgtcaggtaa tggatagacc gtcaccagtg ccgacttcgc cagacgcaaa
     5701 atgggttctc gttcacgtaa cgggcagcaa tacactggat gtgccgccga aagggtcatt
     5761 acgccagaag gatgttgtgg cagcacgaaa gcgggtacct gatcggcggc aagatagtta
     5821 gcggtcgcaa actgtaccaa ctctggcgtt gtttgcgctt cctgcaataa caccagatgt
     5881 gcgtctttgc cgtaattctt taataccgac aaccattcag cgcgttgctg tttgtatatg
     5941 ttccacacca ggatccgaat acgctcttcg gtacttaacg gttctccggg tggtaatgcc
     6001 tggccgatgc tcgcaaaaga ccccggcggt aagatcctct ccgcaggttg tccggcaaca
     6061 tagcgcatgg cataggtgtt ttttcgcact tttgactttg aaacctctat tacgtcaacc
     6121 cttccgcact cgggagggtt ctactgttat agggatttta gctcactctt tcaacgagca
     6181 attgtatatt gttatgtaag caagtgctta caccggtcag aaatttgctc attattgaga
     6241 acattaaatc ggatgacata tcacgcatcc tgatggttta atgacaaata aattcaaaat
     6301 atctcatcta ttttgcaaca ggagcaaaaa tgaaagccac gtcggaagaa ctcgccattt
     6361 ttgtttcggt cgtcgaaagc ggcagcttta gccgggcagc ggaacaatta gggcaagcaa
     6421 actcagcggt aagccgggcg gtgaaaaagc tggaaatgaa acttggcgtt agcctgctta
     6481 atcggaccac gcggcaactt agcctgacgg aagaaggcga gcgttatttt cgtcgcgtac
     6541 agtcaatttt gcaggagatg gcagcggcag aatcagaaat tatggagacg cgtaatacac
     6601 cgcgtggact gttacggatc gatgccgcaa ctccagtggt gctgcacttt ctgatgccgt
     6661 taattaagcc tttccgtgaa cgctatccgg aagtcacttt atcgctagtc tcctccgaaa
     6721 cgattattaa tttgatcgaa agaaaagtgg atgtcgcgat acgcgctggt acgttaacgg
     6781 actccagctt acgtgccagg ccgttattta acagttatcg aaaaattatc gcctcccccg
     6841 attatatttc ccgctacggg aagccagaaa cgatcgacga tttaaagcaa catgtttgcc
     6901 tgggattcac tgaacccgct tccctcaata cctggccgat agcctgtagt gatggacaat
     6961 tacatgaggt gaagtacggt ttgtcatcca atagtgggga aacactgaaa cagctttgcc
     7021 tgagtggcaa cgggattgcg tgtttgtccg actacatgat cgacaaagaa atcgctcgcg
     7081 gagaattggt ggagttaatg gcagataaag tgttgccagt ggaaatgcca ttcagtgccg
     7141 tctattacag cgaccgtgcg gtaagtacgc gcatccgggc ttttatcgat ttccttagcg
     7201 agcatgtaaa aacagctccc ggaggagctg tcagagaggc ttaatcccat tcaggagcca
     7261 gaccttccgg gctaaccagg cggtcgttgc aatccagtgc ggcgatcgct tttttatctt
     7321 cggcatcaag ctgtaaattt tgtgccttaa gattactttc caggttttta cgtttagtag
     7381 aagaaggaat tactgagtaa ccttccccca tagcccacgc cagaatcact tgtgccggag
     7441 tcgcattgtg tttagctgca atacgagcaa taacctcatc tttcagtgcc ttaccataag
     7501 ccagcgtcat ataggaagtg atatgaatac cgtgctgttt agcccaggca accactttac
     7561 ggttttgcag ataaggagag agttcaatct ggttggtagc gatgttttca gcgccaacag
     7621 cagcaatcgc tttttccatc aacgggatcg tgaagttgga aataccgatc tcacgtgtca
     7681 gaccttgttt tttggcctcc agcagcgcct gcataaactc ttcaacagag acttcatcgt
     7741 ttggtgacgg ccagtggatt agcgtcagat caacataatc ggtacgcaat ttttgcaggc
     7801 tctcttttag actcgggatc aatttgtctt tgctgagatt ttcaatccag attttagtgg
     7861 tgatgtagag ttcatgacgt ggcacgccac tttctgcaat cgcctgacct actgcggctt
     7921 cgttatcata gatttgtgca gtatcaattg cgcgataacc aagttcaagc gccgttttca
     7981 cagatgaaat aacaacgtcg tctttgagac ggaaagtacc taaaccaaat gcagggatag
     8041 ccataatatg cctcttttaa tgctattttc gttaactgag gtgattatgg gcttcagact
     8101 gttgcggaaa aagagcataa aatgcagagg atttttgcga ttctggcaat aatagatata
     8161 cagacgaaaa aaaacctcga gcgttaactc aaggcttctt aataagtggc ggaacggacg
     8221 ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact gaactaccgc
     8281 tccaccgaat tcttttacaa ccaccggttt tatgaccggc ttactgctta atttgatgcc
     8341 tggcagttta tgaatcactt cgtgattcac ccttcgggcc gttgcttcgc aacgttcaaa
     8401 agctttcgct tttgtcctac tctcttatcg agtagtgaac tgcctcaaag gtcagtgcca
     8461 ttcttc
//
LOCUS       DAZOZY010000172         8375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZOZY010000172 DAZOZY010000000
VERSION     DAZOZY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.173"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(8..214)
                     /locus_tag="ACHURQ_003343"
     CDS             complement(8..214)
                     /locus_tag="ACHURQ_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR0825055.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            527..616
                     /gene="kdpF"
                     /locus_tag="ACHURQ_003344"
     CDS             527..616
                     /gene="kdpF"
                     /locus_tag="ACHURQ_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR0825056.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            616..2289
                     /gene="kdpA"
                     /locus_tag="ACHURQ_003345"
     CDS             616..2289
                     /gene="kdpA"
                     /locus_tag="ACHURQ_003345"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR0825057.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2312..4360
                     /gene="kdpB"
                     /locus_tag="ACHURQ_003346"
     CDS             2312..4360
                     /gene="kdpB"
                     /locus_tag="ACHURQ_003346"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR0825058.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4369..4941
                     /gene="kdpC"
                     /locus_tag="ACHURQ_003347"
     CDS             4369..4941
                     /gene="kdpC"
                     /locus_tag="ACHURQ_003347"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR0825059.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4934..7618
                     /gene="kdpD"
                     /locus_tag="ACHURQ_003348"
     CDS             4934..7618
                     /gene="kdpD"
                     /locus_tag="ACHURQ_003348"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR0825060.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7615..8292
                     /gene="kdpE"
                     /locus_tag="ACHURQ_003349"
     CDS             7615..8292
                     /gene="kdpE"
                     /locus_tag="ACHURQ_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR0825061.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1740 a         2181 c         2437 g         2017 t
ORIGIN      
        1 tcagcaatca gtaaaaatca ccggttgcct tttcggcctg atccatccac accggtttat
       61 cgctggtttt cgaccagacg cggtgcaggt agctgtaaaa gcgggcgcgg tctttccaga
      121 aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc atatgtacgg cgtaaaaaga
      181 taagccatgc aggatattct ctgtagagtt ccatactttt ctcccccttt tgtatctacc
      241 cggtgaatag caccggaaaa atgaatttgt ttatctgatg aaaatagtac cgccttttgt
      301 gtaattttac tactcatccg accacttatt tttgcttatt gatggtttat ttacattcac
      361 tctgtaatta agttacacaa aagttaaatt aatactaaac attagttaaa tcatggcttt
      421 tgccattttt atactttttt tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca
      481 gaattctact cttccggtat cacttttagg ccactggagg tgcactgtga gtgcaggcgt
      541 gatagccggc gtgttgctgg tgtttttatt actgggttat ttggtttatg ccctgatcaa
      601 tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga ttgccacgtt tttactggta
      661 ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc ggctgattaa tgacattcct
      721 cttccaggta cagcgggcgt tgaacgtata ctctttcgtt tacctggcgt ctctgaccat
      781 gagatgaact ggaagcaata tctttgtgcc attctcggcc tgaacatgct ggggctggcg
      841 gtgctgtttt ttatgttgct cggtcagcac tatctgccgc ttaatccaca gcagttgccg
      901 gggctgtcgt gggatctggc gctgaatacc gccgtcagct ttgtcacgaa taccaactgg
      961 caatcttata gcggtgaaac cacgttgagc tatttcagcc agatggcagg cttaacggta
     1021 caaaactttc tttctgccgc cagcgggatt gcggtgattt ttgccttcat ccgtgcgttt
     1081 actcgccaga gcatgagcac actcgggaat gcctgggtcg atctgctacg catcacgtta
     1141 tgggtgctag tccctgtggc gttgttgatc gcactgtttt ttattcaaca aggtgcgctg
     1201 caaaactttc tgccctatca ggcagtgaat accgttgaag gagcgcaaca gctgttaccc
     1261 atgggaccag tagcttctca ggaagcgatc aagatgctcg gtactaacgg cggtggcttc
     1321 tttaatgcca actcgtcgca tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag
     1381 atgctggcga tcttcttgat cccaacagcg ctgtgctttg cctttggtga agtgacgggc
     1441 gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt catctgcgta
     1501 ggcgtggtta tgtgggcaga agttcagggt aatcctcatc tgctggcgct cggcgcagac
     1561 agcagcatca atatggaagg taaagagagc cgtttcggcg tgctggtcag tagcctgttt
     1621 gcggtcgtga ctacggcggc gtcctgtggc gcggtgattg cgatgcatga ttcgtttacc
     1681 gctctcggcg gcatggtgcc gatgtggcta atgcaaattg gtgaagtggt gttcggcggt
     1741 gtcggttctg gtctttacgg catgatgctg tttgtcctgc tggcggtgtt tattgccggg
     1801 ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa
     1861 ctgattgcac tggcgattct ggttactcca acactggtac tgatgggcgc ggcgttggcg
     1921 atgatgaccg acgccggacg tagcgccatg cttaaccctg gcccgcatgg ttttagcgaa
     1981 gtgctgtacg ccgtgtcgtc cgccgctaac aacaacggca gcgcctttgc cggattaagc
     2041 gccaactctc cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg
     2101 gtgattatcc cggtgatggc aattgccggt tcgctggtga gtaaaaagag ccaacccgcc
     2161 agctccggca cgctgccaac gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg
     2221 ttgctggttg gcgcactgac ctttatccct gccctggcgc ttggtccggt ggcggaatat
     2281 ctctcctgat gatattgagc gagcactgaa tatgagtcgt aaacaactgg cgctattcga
     2341 accaacactt gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca
     2401 atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca cctgtattag
     2461 catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg tttagcgcgg caattagcgg
     2521 ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag aaggccgcag
     2581 taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact gcctttgccc gcaagctgcg
     2641 tgagccgaaa tatggcgctg cggcggacaa agttcctgcc gatcaactgc gtaaaggtga
     2701 catcgtgctg gtagaagctg gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg
     2761 cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg
     2821 cggcgatttt gcctccgtca ccggcggcac gcgtattctt tctgactggc tggtgattga
     2881 gtgtagcgtt aaccccggtg agacatttct ggatcggatg atcgcgatgg tggaaggcgc
     2941 acagcgacgc aaaacaccga acgagattgc cctgactatc ctgctgattg ctctgaccat
     3001 cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg gtaatgccgt
     3061 gagtgtaacg gtactggtgg cgctgctggt ctgtctgatc ccaaccacta ttggcggcct
     3121 gttgtcagcg atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg tgattgccac
     3181 cagcggacgt gcagttgaag cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg
     3241 caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc cccgcgcagg gcgtggatga
     3301 aaaaacgctg gctgacgccg cacaactggc ttcgctggct gatgaaacgc cggaaggtcg
     3361 cagtattgtg atcctcgcca agcaacgttt taacctgcgc gagcgcgatg tgcagtcgct
     3421 ccatgccacc tttgtaccgt ttactgcgca aagccggatg agcgggatca acatcgacaa
     3481 ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc catattgagg ctaacggcgg
     3541 tcacttccct gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg gagccacgcc
     3601 gctggtggtg gtggaaggtt ctcgtgtgct gggcgttatt gcgctgaaag atatcgtcaa
     3661 aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg ggcattaaaa cggtgatgat
     3721 taccggcgat aaccgtctga ctgccgccgc gatagctgcg gaagcgggtg tcgatgattt
     3781 tctcgccgaa gcaacaccgg aggccaagct ggcattgatc cgtcagtatc aggcggaagg
     3841 tcgtttggta gcgatgaccg gcgacggcac caacgatgct ccggcgctgg cgcaggcaga
     3901 tgtcgcggtg gcgatgaact ccggcaccca ggcggcgaaa gaggcgggca atatggtgga
     3961 tctcgactct aacccgacca agttgatcga ggtggtgcac attggcaaac agatgctgat
     4021 gacccgtggc tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat
     4081 tattccggcg gcgttcgcgg caacatatcc gcagttaaat gcgctgaaca tcatgcgcct
     4141 gcattcgccc gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt
     4201 tttgattcct ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt
     4261 gcgccgtaac ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa
     4321 agtcattgat ttactgctga ccgtttgcgg tctggtgtga ggtttaccat gagtggatta
     4381 cgtccggcat tatcgacatt tctctttctg ttattgatta ctggcggcgt ttacccgctg
     4441 ctgaccaccg cactggggca atggtggttt ccctggcagg ccaacggttc gttgatccgt
     4501 gaaggtgata cggtgcgtgg ctcggcatta atcgggcaga attttaccgg caacggctat
     4561 tttcatggtc gcccgtcagc aacggctgaa atgccctata atccacaggc ttctggcggg
     4621 agcaatctgg cggtcagtaa ccctgagctg gataaactaa tagccgcacg cgttgctgca
     4681 ttacgggccg ctaacccgga tgccagcgcg agcattccgg ttgaactggt gacggcatcg
     4741 gcaagcgggc tggacaataa tatcaccccg caagcggcgg cctggcagat cccacgcatc
     4801 gcgaaagcgc gtaatctcag cgttgaacag ctcacgcagc tgatcgcaaa atacagccaa
     4861 caaccgctgg tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggcg
     4921 ctggataagc ttgatgaata acgaaccctt acgtcccgac cccgatcgtc tgctggaaca
     4981 aactgccgcg ccgcatcggg ggaagctgaa agttttcttc ggtgcctgtg caggcgtcgg
     5041 gaagacctgg gcgatgctgg cagaagccca gcgactgcgg gcgcaagggc tggatattgt
     5101 ggttggcgtg gtagaaaccc acgggcgaaa agataccgcc gcaatgctgg aagggctggc
     5161 tgttctgccg ccaaaacgcc tggcgtaccg tgggcggcac atctgcgagt ttgacctcga
     5221 tgccgccctc gcccgccgtc cggcgctgat cttaatggac gaactggcgc acagtaatgc
     5281 gccaggttct cgtcacccta aacgctggca ggacatcgaa gagctgctgg aagcaggcat
     5341 tgatgttttc actaccgtca acgttcagca tctggaaagt ctgaatgatg tggtcagcgg
     5401 cgtcaccgga attcaggtac gggaaaccgt gcccgatcct tttttcgatg ccgccgacga
     5461 cgtggttctg gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt
     5521 ctatattgcc gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gcaatctgat
     5581 cgccctgcgc gaactggcac tgcgccgtac tgccgatcgc gttgatgagc aaatgcgcgc
     5641 ctggcggggg catcctagcg aagagaaagt ctggcacacg cgcgacgcga tccttttatg
     5701 catcggccat aacaccggta gtgagaagct ggtccgcgcg gcggcgcggc tggcgtcacg
     5761 gctgggtagc gtctggcacg cggtgtatgt tgaaacccct gccctgcatc gcttaccgga
     5821 gaaaaaacgt cgtgcaattc tcagcgcctt acgtctggcg caggaactgg gcgcggagac
     5881 ggcaacactt tctgatccag cggaagagaa agcggtagtg cgttatgccc gtgaacataa
     5941 tctcggcaag attattctcg gtcgcccggc ctcgcgccgc tggtggcgtc gggaaacgtt
     6001 tgctgaccga ctggcgcgca tcgcccccga tctcgatcag gtgctggtcg cgcttgatga
     6061 accacccgcc cgcacgatta acaacgcgcc ggatagccgc tcttttaaag acaagtggcg
     6121 ggtacagatt cagggatgcg tggttgccgc cgcgttatgc gccgttatca ccttaattgc
     6181 catgcagtgg ctgatggcgt ttgatgccgc caacctggtg atgctgtatc tgcttggcgt
     6241 ggtggtggtg gcgctatttt atggacgctg gccttcggtg gttgccaccg tcattaatgt
     6301 ggtgagtttc gatctctttt ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca
     6361 atatctgctg accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc
     6421 tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgag caacgcacac ggcacttata
     6481 tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag gacatcgccg ccaccagcga
     6541 acaatttatt gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg
     6601 taaattgcag ccgttaacac atccgcaagg aatgacgcca tgggacgatg ccatcgcgca
     6661 gtggagtttt gataaaggcc tgcctgcggg cgcgggcacc gacacgttac ccggtgtacc
     6721 gtaccagatt ttgccgctaa aaagcggcga gaaaacctac gggctggtgg tggtggagcc
     6781 ggggaatctg cgccagttga tgatcccgga acagcagcgc ctgctggaga cgtttacgct
     6841 gttagtcgcc aatgcccttg agcggctgac gctaaccgcc agcgaagaac aggcgcggat
     6901 ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggca gcgctttcgc atgatttacg
     6961 cacgccgctt acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga
     7021 aggatcaccc cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg
     7081 actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa
     7141 agagtggtta acgctggaag aagtagtcgg cagcgcgctg caaatgctgg aaccgggttt
     7201 atcgtcgccc atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc
     7261 actctttgaa cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca
     7321 ggccgaaatt ggtatcgatg cccactttga gggcgaaaat ctacaactgg atgtctggga
     7381 taacggcccc ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg
     7441 gaataaagag tcggcggtgc cgggtgtcgg gcttgggttg gcaatttgtc gggcgattgt
     7501 ggatgtacac ggcggcacca ttaccgcgtt caaccgaccg gaaggtggtg cctgttttcg
     7561 tgttacactt ccccagcaaa ccgctcctga acttgaagac tttcatgagg atatgtgaca
     7621 aacgttctga ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac ggcgctggag
     7681 ggcgacggga tgcgcgtctt tgaggccgaa actctgcaac gcggcttgct ggaagcggca
     7741 acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag
     7801 tttatccgcg acctgcgcca gtggagcccg gtgccggtga ttgtgctttc cgcacgcagc
     7861 gaagagagcg acaagatcgc cgcgctggat gccggagcgg atgattatct gagtaagccg
     7921 tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccacc
     7981 gccgcgcccg atccgctggt gaaattttcc gatgttaccg tcgatttagc cgcccgcgtg
     8041 attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct gctggcggtg
     8101 ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca ggtgtggggg
     8161 ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct gcgacaaaaa
     8221 ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat tggatatcgg
     8281 tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt tattattaaa
     8341 cctgcgccga atattattat ttggcaggtt taatt
//
LOCUS       DAZOZY010000173         8343 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZOZY010000173 DAZOZY010000000
VERSION     DAZOZY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.174"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            90..1586
                     /gene="ravA"
                     /locus_tag="ACHURQ_003350"
     CDS             90..1586
                     /gene="ravA"
                     /locus_tag="ACHURQ_003350"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR0825062.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1580..3031
                     /gene="viaA"
                     /locus_tag="ACHURQ_003351"
     CDS             1580..3031
                     /gene="viaA"
                     /locus_tag="ACHURQ_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR0825063.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHURQ_003352"
     CDS             complement(3036..4028)
                     /gene="asnA"
                     /locus_tag="ACHURQ_003352"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR0825064.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4180..4638
                     /gene="asnC"
                     /locus_tag="ACHURQ_003353"
     CDS             4180..4638
                     /gene="asnC"
                     /locus_tag="ACHURQ_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR0825065.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4728..5171
                     /gene="mioC"
                     /locus_tag="ACHURQ_003354"
     CDS             4728..5171
                     /gene="mioC"
                     /locus_tag="ACHURQ_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR0825066.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHURQ_003355"
     CDS             5550..7439
                     /gene="mnmG"
                     /locus_tag="ACHURQ_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR0825067.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHURQ_003356"
     CDS             7503..8126
                     /gene="rsmG"
                     /locus_tag="ACHURQ_003356"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR0825068.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2112 a         2121 c         2115 g         1995 t
ORIGIN      
        1 cccccctttg agactatttt agccaccagc aagaaacgtc tatactcgca atttacgcag
       61 aacttttgac gaaaggacgc cacttcatta tggctcaccc tcatttatta gcggaaagaa
      121 tttcccgcct gagcagttcg ttggaaaagg ggctttatga acgtagccac gccatccgct
      181 tgtgtttatt agcggcatta agtggtgaaa gtgtgttcct ccttggcccg ccaggtattg
      241 ccaaaagttt gatcgcccgg cgcttaaaat tcgcctttca gaatgcccgc gcgtttgaat
      301 atctgatgac ccgcttctcc acgccggaag aagtttttgg tcccctttct attcaggcgc
      361 taaaagatga agggcgctat gaacgtttaa ccagcggtta cctgccggaa gcagaaattg
      421 tctttctgga tgagatctgg aaagcgggcc cggcaattct taataccttg ctcaccgcca
      481 ttaacgagcg ccagttccgc aacggtgcac acgtagaaaa aatcccgatg cgtctgctgg
      541 tggcggcctc caacgagctg ccggaagcag acagcagtct ggaagcgtta tatgaccgca
      601 tgctgattcg tctgtggtta gataaagtgc aggataaagc gaatttccgc tccatgctga
      661 ccagtcaaca ggatgaaaac gacaatccgg ttcctgatgc cttgcaggtc acagatgaag
      721 aatatgaacg ctggcagaaa gagattggtg aaattacgct gcccgatcat gtatttgagc
      781 tgatttttat gctgcgccag caactggata aattaccgga tgcgccttat gtctcggatc
      841 gtcgctggaa aaaagcgatc cgattattgc aggccagcgc cttttttagc ggtcgcagtg
      901 ctgttgcccc ggtagatctc attttgctga aagattgcct gtggtatgac gcgcaaagcc
      961 tgaatttgat acaacaacaa attgatgtat tgatgaccgg tcatgcctgg caacagcaag
     1021 ggatgttgac tcgccttggc gcgattgtgc aacgtcacct gcaactccag cagcaacaaa
     1081 gcgataaaac agccttaacg gtaattcgtc tgggcggcat tttcagccgt cgtcagcagt
     1141 atcaacttcc tgttaacgtt actgcttcca ctctgactct actgctgcaa aaaccgttaa
     1201 aactgcatga tatggaagtg gttcatatct cctttgagcg tagcgcgctg gaacagtggc
     1261 tgagcaaagg tggtgaaatt cgcggtaaac tgaacggtat cggctttgcc cagaaactga
     1321 atctggaagt tgatagcgcc caacatcttg tcgttcgcga tgtaagttta caaggcagta
     1381 cgctggcact tcccggttca tcggctgaag gtctgcctgg tgaaataaaa caacaactgg
     1441 aagagcttga aagcgactgg cgcaagcaac acgctttatt cagcgaacag caaaaatgtc
     1501 tgtttatccc tggcgactgg ttaggtcgca ttgaagccag cctacaggat gtcggtgcac
     1561 agattcacca ggcacaacaa tgctaacgct ggatacgctt aatgtgatgc tggccgtcag
     1621 cgaagaggga ttgatcgaag agatgatcat cgcactgctg gcctcaccgc agctggcggt
     1681 cttctttgaa aaattcccac gcctgaaggc ggcaatcact gatgatgttc cccgctggcg
     1741 tgaggcgctg cgcagtcggc tgaaagatgc ccgagtcccg ccggaactca ccgaagaggt
     1801 gatgtgctat cagcaaagcc agctcctctc cacgccgcag tttattgtgc agctaccaca
     1861 gatcctggac ttactgcatc gtctgaattc cccatgggca gaacaagccc ggcagttggt
     1921 tgatgctaac agcacaataa cttcagcgtt acacacgctt tttctccagc gttggcgttt
     1981 aagtctgatc gtgcaagcaa cgacgttaaa tcaacagcta ttagaagaag aacgcgaaca
     2041 actgttgagt gaagttcaag aacgcatgac gctgagcgga caacttgaac cgattctcgc
     2101 agataacaat actgcagctg gtcgtctgtg ggatatgagc gccggtcagc ttaaacgtgg
     2161 cgactatcag ttgattgtga aatacggtga atttcttaac gaacagccgg aactgaaacg
     2221 cctggcagag cagctggggc gttctcggga agccaaatca ataccgcgca acgatgcgca
     2281 gatggaaacc ttccgcacca tggtgcgcga accggcgacg gttcctgagc aggttgatgg
     2341 tctgcaacaa agcgatgata ttttacgtct tctgccgcca gaactggcga cactagggat
     2401 aacggaactg gagtatgagt tttaccgtcg gctggtggaa aaacagttgc tcacctatcg
     2461 cctgcacggt gagtcgtggc gtgaaaaagt gatcgaacgt ccggtggtac ataaagatta
     2521 cgatgaacag ccgcgcgggc cgtttattgt ctgtgtggat acttccggct caatgggcgg
     2581 ctttaatgaa cagtgtgcga aagcgttctg cctggccttg atgcgcattg ctctcgctga
     2641 aaaccggcgc tgctatatta tgctattttc caccgagatc gtccgttatg agctttcagg
     2701 cccccaaggc atcgaacaag caatccgttt tttaagccag cagtttcgtg gcggcaccga
     2761 tcttgccagt tgttttcgcg ccattatgga acgcttgcaa agcagggaat ggtttgatgc
     2821 cgatgcggtg gtgatttctg attttatcgc tcagcggttg cctgacgacg tgacgagtaa
     2881 agtgaaagag ctgcagcggg tacatcagca tcgctttcat gccgtggcga tgtcggcaca
     2941 cggcaaaccc ggcatcatgc gcattttcga tcatatctgg cgctttgata ccgggatgcg
     3001 aagccgcctg ctcagacgct ggcggcgata aattattaca gcagagaagg gacgttctcg
     3061 cgaacagcag ctggccatac accacactga acctggccga tatgcggcag ttgcagcagc
     3121 agcatggtca aacgagactg gccgataccg ccgccgatgg tctgcggcat ttcaccgcgc
     3181 agcagcgcct gatgccattc cagctgcaga cgatcttcat caccggtcag cgccaactga
     3241 tgcttcaacg tgtcggcatc cacgcggatc cccatggaag aaagctcaaa cgcatcttcc
     3301 agtacagggt tccacaccag aatatcgccg ttcagacccg catgacccag ctctgacggg
     3361 gtgctccagt catcataatc cggtgcgcgc acgtcgtggc gatgaccatc gctcagcttg
     3421 ccgccaatcc cgacaaggaa taccgcgcca agatctttcg ctatcgcccg ctcacgccct
     3481 ttggcatcga gatccggata acgagacagt aactcctggc tgtgtacgaa gtggatctga
     3541 tccggcagga acggtgccag gccaaactct tcgctaaccg cagcttcggt tgctttaatt
     3601 cccgcccaga tcgcctctac cgtgcttttc agagtcgaga attgacgctc accgtcgccc
     3661 attacgcgtt cccagtccca ctggtcaaca tagaccgagt gcaacggaga aagacggtct
     3721 tcatcggggc gaagggcttt catgtgcgtg tacagccctt cgcccgcgct gaagtcgtgt
     3781 tgccctaagg tctgacgttt ccacttcgcc agtgaatgaa ccacttcgaa ctgggcatca
     3841 ggcagagctt tcacttttac ctgcaccgct ttttcacagc ccgacaagtt atcctgcgtg
     3901 ccatccccca cacggctaag aatcggcgcc tggacttcga tcagccccag acgttcttcc
     3961 agttggcgag aaaagtgaga tttcacgaag ctaatttggc gttgtttggc aatgtaagcg
     4021 gttttcattt tttatactcc tgcgtcctgt tgcttatgat taagcaacaa aaaccgacaa
     4081 acatgcaata atcattcaat aaaaagccta acacactttt gattcattaa aaaagaaggc
     4141 taaaatagaa tgaatcatca atctgcataa gaaaatccta tggaaaatta tctgatcgac
     4201 aatctggacc gtggcatcct ggaagcatta atgggcaatg cgcgcaccgc ttacgccgaa
     4261 ctggcgaaac aatttggcgt cagtccgggg acgattcacg ttcgagtaga gaaaatgaag
     4321 caggcgggga tcattaccgg ggcgcgtatt gatgtcagcc cgaagcagct cggttatgac
     4381 gtaggctgct ttatcggcat tatattaaag agcgccaaag actacccttc cgcgctggca
     4441 aagctggaaa gccttgctga agtcactgaa gcctattaca caacaggcca ctacagcatc
     4501 tttataaaag taatgtgccg ttcgatcgac gctctccagc atgtacttat caacaagatc
     4561 caaacaattg atgaaattca gtccaccgag acattgatcg tcctgcaaaa cccgatcatg
     4621 cgtaccatca agccataatc ggctttttta atcccatact tttccacagg tagatcccaa
     4681 cgcgttcaca gcgtacaata cgccactctt aataaaggtg gcggtttatg gcagatatca
     4741 ctcttatcag cggcagcacc ctcggcggtg ccgaatatgt agcagaacac ctggctgaaa
     4801 agctggaaga ggcgggtttt accaccgaaa cgctgcacgg tccgctgttg gaagatttat
     4861 ctgcctcggg gatctggctg attatcagct ccacccacgg tgccggagat attccggaca
     4921 acctttctcc tttctatgaa gcattgcagg aacagaagcc cgatctttct gcggtccgct
     4981 ttggcgcaat cggtattggt agtcgtgaat atgacacctt ttgtggggct atcgataaac
     5041 tcgaggcaga actcaaaaat tccggtgcaa aacagacagg cgaaacactg aagatcaaca
     5101 ttcttgatca cgacattccg gaagatccgg cagaagaatg gctgggatcg tggattaatt
     5161 tactcaaata agtatacaga tcgtgcgatc tactgtggat aactctgtca ggaagcttgg
     5221 atcaaccggt agttatccaa ggaacaacgg ttgttcagtt tttgagttgt gtataacccc
     5281 tcattctgat cccagcttat acggtccagg atcaccgatc attcacagtt aatgatcctt
     5341 tccaggttgt tgatcttaaa agccggatcc ttgttatcca cagggtagtg cgatcctaat
     5401 aagagatcac aatagaacag atctctaaat aaatagatct tctttttaat acccaggatc
     5461 ccaggtcttt ctcaagccga caaagttgag tagaatccac ggcccgggct tcaatccatt
     5521 ttcataccgc gttatgcgag gcaatcacca tgttttatcc ggatcctttt gacgtcatca
     5581 tcattggcgg gggtcatgca ggcaccgagg ccgcgatggc cgcggcgcgt atgggtcaac
     5641 agactctgct tttgacacac aatatcgaca ctctggggca gatgagctgc aacccggcga
     5701 tcggcggtat tgggaaggga catctggtaa aagaagtgga tgcactcggc ggtctgatgg
     5761 cgaaagcgat cgatcaagcg ggtatccagt ttaggatact aaacgcaagc aaaggaccgg
     5821 cggttcgcgc tacccgagct caggcggatc gtgtgctcta ccgtcaggcg gtacgtacgg
     5881 cgctggagaa ccaaccgaac ctgatgatct tccagcaggc ggttgaagat cttattgtcg
     5941 aaaacgatcg cgtggtcggt gctgttaccc aaatgggact gaagttccgt gccaaagccg
     6001 tcgtgctcac cgttgggacg ttcctcgacg gtaaaattca tatcggtctg gataattaca
     6061 gcggtggtcg tgctggtgat ccgccgtcca ttccgctttc tcgccgtttg cgtgaactgc
     6121 cgctgcgcgt tggtcgtctg aaaaccggga caccaccgcg tattgatgct cgaactatcg
     6181 actttagtgt gctggcgcaa cagcatggcg ataacccaat gccggtattc tcgtttatgg
     6241 gcaatgcgtc ccagcatccc cagcaggtgc cgtgttatat cactcatacc aacgagaaaa
     6301 cccatgatgt gatccgcagt aacctcgatc gtagcccaat gtacgcaggg gtgatcgaag
     6361 gtgtcggccc acgctactgc ccatcgatcg aagacaaagt catgcgcttc gccgacagaa
     6421 atcagcatca gatcttcctt gaaccggaag ggctgacctc taacgaaatt tatccgaacg
     6481 gtatctccac cagcctgccg ttcgatgtgc agatgcaaat cgtccgctcc atgcagggga
     6541 tggaaaacgc gaagatcgtg cgtccgggtt atgccattga gtatgacttc ttcgatccac
     6601 gcgacctgaa accgacgctg gagagcaagt ttatccaggg gctgttcttt gctggtcaga
     6661 ttaacggcac taccggttac gaagaagccg ctgcgcaagg tttgctggtc ggtcttaacg
     6721 ctgcccgtct gtctgctgac aaagaaggtt gggctccggc gcgttctcag gcgtatctcg
     6781 gcgtactggt tgatgacctg tgcactttag gaaccaaaga accgtatcgt atgtttactt
     6841 cgcgcgcaga atatcgtctg atgctgcgcg aagataatgc ggatctgcgt ttgactgaaa
     6901 tcggtcgtga actgggcctg gtggatgacg aacgttgggc gcgctttaac gagaaacttg
     6961 agaatatcga gcgtgagcgt cagcgtctga aatcgacctg ggtaaccccg tcggcggaag
     7021 ctgcagccga agtgaatgct cacctgactg cgccgctttc ccgtgaagcc agtggtgaag
     7081 atctgctgcg tcgtccggaa atgacttatg aaaaattaac cacgctgacg ccgtttgccc
     7141 ctgcgttgac agacgaacag gcggcggaac aggttgagat tcaggttaaa tacgaaggtt
     7201 atatcgcgcg ccagcaagat gagatcgaaa agcagctgcg taacgagaac accctgctac
     7261 ccgcgacact ggattaccgc caggtatccg gtctttctaa cgaagtgatc gccaaactta
     7321 acgatcacaa accggcctct atcggccagg cttcgcgtat ttctggcgtc acgcctgcgg
     7381 ctatctccat tctgctggtg tggctgaaaa aacagggtat gctgcgtcgt agcgcataac
     7441 gcattaaaaa tgcctggtaa gcacccgctt accaggcaac gcatcaagaa caggtaatca
     7501 ccgtgctcaa caaactctcc ttactgctga aagacgcagg tatttcgctt accgatcacc
     7561 agaaaaacca gcttattgcc tacgtgaata tgctgcataa atggaacaaa gcgtacaacc
     7621 tgacttcggt ccgcgatcct aatgagatgc tggtacgcca tattctcgat agcattgtgg
     7681 tggcaccgta tctgcaaggt gaacggttta tcgatgtcgg caccggacca ggactgccag
     7741 gcattccact ctctatcgtg cgtcctgaag cccatttcac tctgttggat agccttggta
     7801 aacgcgtgcg tttccttcgt caggtgcaac atgagcttaa actggagaat attgagccag
     7861 tacagagcag ggtagaagag tttccttcag agccgccatt tgatggcgta attagccgcg
     7921 cttttgcctc tctgaacgat atggtgagct ggtgccacca tcttcctggt gagcaaggcc
     7981 gtttctacgc gctgaaaggg caaatgccgg aagatgaaat cgctttgttg cccgaagaat
     8041 atcaggtcga atcagtggtt aaacttcagg ttccagctct ggatggcgaa cgtcatctgg
     8101 tggtgattaa agcaaataaa atttaatttt tatcaaaaaa atcataaaaa attgaccggt
     8161 tagactgtta acaacaacca agttttctac tgatataact ggttacattt aacgccacgt
     8221 tcactctttt gtatcaacaa gataacgtgg ctttttttgg taagcagaaa ataagtcatt
     8281 agtgaaaata tcagtctgct aaaaatcggc gctaagaacc atcattggct gttaaaacat
     8341 tat
//
LOCUS       DAZOZY010000174         8332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZOZY010000174 DAZOZY010000000
VERSION     DAZOZY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.175"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..189
                     /locus_tag="ACHURQ_003357"
     CDS             <1..189
                     /locus_tag="ACHURQ_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825069.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYAL
                     FPIFTASWRATRIKNIGI"
     gene            412..909
                     /gene="ubiC"
                     /locus_tag="ACHURQ_003358"
     CDS             412..909
                     /gene="ubiC"
                     /locus_tag="ACHURQ_003358"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR0825070.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            922..1794
                     /gene="ubiA"
                     /locus_tag="ACHURQ_003359"
     CDS             922..1794
                     /gene="ubiA"
                     /locus_tag="ACHURQ_003359"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR0825071.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHURQ_003360"
     CDS             complement(1949..4372)
                     /gene="plsB"
                     /locus_tag="ACHURQ_003360"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR0825072.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHURQ_003361"
     CDS             4543..4911
                     /gene="dgkA"
                     /locus_tag="ACHURQ_003361"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR0825073.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5021..5629
                     /gene="lexA"
                     /locus_tag="ACHURQ_003362"
     CDS             5021..5629
                     /gene="lexA"
                     /locus_tag="ACHURQ_003362"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR0825074.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5702..7027
                     /gene="dinF"
                     /locus_tag="ACHURQ_003363"
     CDS             5702..7027
                     /gene="dinF"
                     /locus_tag="ACHURQ_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR0825075.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7143..7352
                     /locus_tag="ACHURQ_003364"
     CDS             7143..7352
                     /locus_tag="ACHURQ_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR0825076.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHURQ_003365"
     CDS             complement(7394..7909)
                     /gene="zur"
                     /locus_tag="ACHURQ_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR0825077.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8227..>8332
                     /gene="yjbL"
                     /locus_tag="ACHURQ_003366"
     CDS             8227..>8332
                     /gene="yjbL"
                     /locus_tag="ACHURQ_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0825078.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRG"
BASE COUNT         1919 a         2012 c         2261 g         2140 t
ORIGIN      
        1 gatatgttga atcctacaaa agataccaat tggaattcca cgtgtatcta taaatccaga
       61 cataagatgt tgcctattaa tcttactcag gaaacacttt ttagctccaa atctcatggt
      121 aaatatgcgc ttttcccaat ttttactgcg agttggcgag ctactcgtat aaagaatata
      181 ggtatttaag taaaggaaaa catcaccgtt cctggcatca tgggcggtga tgcccctacg
      241 gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc
      301 atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa tttgctttac atctcccgta
      361 aacacttttc tgcgatacaa tgcctttacg ttatgtaacg gagagttcgg catgtcacac
      421 cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag agatccctgc cctggattcg
      481 caactgctcg actggctgtt gctggaagat tccatgacaa aacgttttga acagcaggga
      541 aaaacggtga gcgtgacgat gatccgcgaa gggtttgtcg agcagaatga aatccccgaa
      601 gaactgccgc tgctgccgaa agagtctcgt tactggttac gtgaaatttt gttatgtgcc
      661 gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg
      721 gagctggcgt tacaaaaatt gggtaaaacg ccgctaggac gctatctgtt cacatcatcg
      781 acattaaccc gggactttat tgagataggc cgtgatgccg gactgtgggg gcgacgttcc
      841 cgcctgcgat taagcggtaa accgctgttg ctaacagaac tgtttttacc ggcgtcaccg
      901 ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc agaataagct gctggcgttt
      961 catcgcttaa tgcgtacgga taagccaatt ggcgcgttac tgctgctctg gccaacatta
     1021 tgggcgttgt gggtggcgac accgggcgtt ccccagctct ggatcctggc ggtgtttgtc
     1081 gcgggtgtct ggctgatgcg cgctgccgga tgtgtggtga atgattatgc tgaccgcaag
     1141 tttgatggtc atgttaagcg cacggcgaac cgaccgcttc ccagcggcgc ggtaacggag
     1201 aaagaggcgc gcgcgctgtt tgtagtgctg gtattgattt catttttact ggtgctgacg
     1261 ctgaatacga tgaccattct gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg
     1321 tttatgaagc ggtataccca tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg
     1381 attccaatgg cttttgccgc tgtgagtgag tcggtgccat tgagttgctg gttaatgttc
     1441 ctcgccaata ttctctgggc ggtggcttac gacacgcagt atgcaatggt tgaccgtgac
     1501 gatgatgtga agattggtat taaatccacc gcaatcctgt tcggccaata cgataaattg
     1561 attattggta ttttgcagat tggcgtactg gcactgatgg cgatcatcgg tgagttaaat
     1621 ggcttaggct ggggatatta ctggtcaatt ctggtggctg gcgcgctgtt tgtttatcaa
     1681 caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta aagcatttat gaataataac
     1741 tatgttggtc tggtactgtt tttaggactg gcaatgagtt actggcattt ctgatgatgt
     1801 aaaaaagccg gatgatcatc cggctttctt ctgggttgcc tgatgcgctg cgcttctcag
     1861 gcctacacaa cacatcgcaa tttattgaat ttgcagatta tggaaggccg gataaggcgt
     1921 tttcgccgca tccggcaatt ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg
     1981 tcaaacgcac gtctgatgta atcaactccg ccagcaactg ataaaccttc atcgtttctg
     2041 ccggttcggc gtcgccgcta tcgctgatat acccttcatc acgcagcgtc agcaccagag
     2101 aactgaacac cgccttgtcg aagaactccg gcgcgttgat gccgtgcagc acggagagac
     2161 gttgcgcaac ggtgcggctc tctttctcca gcgtaccgcg gttgatcgac gggttggcac
     2221 tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca
     2281 gctgtagggg gcgagaatgc gccgggttga tatgcaactc atcatcttgc agggtaatca
     2341 gcccctgacg ttgcatctca tttgccagcg catcaataac gtccggcaac tcgtcgcgat
     2401 cccagcgcag gaacagctcc gctttcagca ttgggtaaag cacattgacg tgctccatca
     2461 atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc cgccatcagc gaaggcagca
     2521 ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag caccgcttgc tcgcgcggca
     2581 gaatgatgat gtcgccgata gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt
     2641 gatcgataag ctcgctggcg ctggctgaag gaacggtaga gtccgtggag taaggtacgt
     2701 tgcgcatcag atccaggtag cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc
     2761 gctgacgtga tgccagaagc gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt
     2821 tgttaatgcg taccatcaga tcggcagcaa tattattgac cgtcggcgtt aaccaggccg
     2881 gacgcaccgc ttcgatggga tcgatagatt cacgccagtc tggtacgtgc tggttaaggt
     2941 aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct
     3001 tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt
     3061 ctttggcgta agtacccact tccatgacgt gctcataacc gatatagatc ggaatcagcg
     3121 taatcggacg cgtgccgcca cgcagcatcg cctgaatggt catcgacagc gtaccggttt
     3181 tcggatccag caaacgcccc gtgcgggaac gaccgccttc cacgaagtac tcgacggaat
     3241 aaccacggct gaacagttcg ccgagatact cacggaaaac ggtggaataa agtttattgc
     3301 ctttaaaagt acggcgaata aagaacgccc ccagacggcg gaaaatcggc ccggccggcc
     3361 agaagttcag gttgatcccg gcggcgatat gcggcggcac cagcccctgg tggtacagca
     3421 cgtaagaaag tagcaggtag tccatgtgac tgcggtggca aggcacatac accagctcat
     3481 gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg gacgttgatg ccctgataaa
     3541 gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg gatcatctcg taagagaaat
     3601 tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg cgctttttca tgggagattt
     3661 ttttgctgcg cgcttcatct tctaccgctt tggcaatggc gcgggaggcg agcagcttat
     3721 taaacagatc ctgacgagca ggcagacgtg ggcctacggc agccagacgt tgacgggcaa
     3781 agtgcatacg cgccacgcgc gccagtttct gtgcgatagt tttatccgtg ccgtgttcat
     3841 ccgccatacg gcgcagcgaa actgacggcg agaaacgcac aaaactgtcg cgaccgagcc
     3901 acagtacggc gaaaaatttc tgtacgccgt taagcatacg cagcggcggg tttacttcgc
     3961 ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac tggcaccatc tgcacatcca
     4021 gatttgggtt gctacggtgc aaatcgagat agtcgtggaa cagcttaata gactcttctt
     4081 tcggcgtgta ataggtgaat acacgcggcc cgccgtgaat gaacacatag cgcggcagta
     4141 gcgtgccgtc gatttctagc ggctctaacg ggtcaggcaa gtcatgagcc agacactggg
     4201 cgcgtaacgt cagcaaatct gctttcgagt tgtacggtaa aacgtacata attggacgag
     4261 aggtatccag ccccagttcc ggggcaggat ctgccggaat agacttgctt tttaccagga
     4321 tgcttaatgg taaattcagt aatttgtagt aaattcgtgg ccagccggac ataaacgatg
     4381 taaagcctct ggttaataat gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt
     4441 ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg
     4501 gtacactgat attgacgctc ataatgtaaa aaggttcttt caatggccaa taataccact
     4561 ggattcaccc gaattatcaa agctgctggc tattcctgga aaggtttacg cgctgcatgg
     4621 atcaacgaag cggcattccg tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc
     4681 tgctggctgg atgtggacgc aattacccgc gtgctgctta tcagctccgt gatgctggtg
     4741 atgattgtgg aaattctcaa tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa
     4801 taccatgagc tttccggacg cgctaaagat atgggatccg ccgcggtgct gattgcgatt
     4861 atcgtcgccg tgataacctg gtgcatcctg ttatggtcgc attttggata acccttccag
     4921 atttcgataa atctctggtt tattgtgcag tttatggttc caaaatcgcc ttttgctgta
     4981 tatactcaca gcataactgt atatacaccc agggggcgga atgaaagcgt taacggccag
     5041 gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta tgccgccgac
     5101 gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg aagaacatct
     5161 gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac gcgggattcg
     5221 tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc
     5281 gcttctggcg cagcagcata ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc
     5341 gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga
     5401 tggtgacttg ctggcagtgc ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc
     5461 acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa cagggtaata aagtcgaact
     5521 gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac
     5581 cattgaaggg ctggcggttg gggttattcg caacggcgac tggctgtaac atatctctga
     5641 gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct ctcttcatca ggcttgtctg
     5701 catggcattc ctcacttcat ctgataaagc actctggcat ctcgccttac ccatgatttt
     5761 ctccaatatc accgttccgt tgctgggact ggtcgatacg gcggtaattg gtcatcttga
     5821 tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg gcgaccagct ttctctttat
     5881 gctgttgctg tttttacgca tgagcaccac cgggctgact gcgcaggctt atggtgccaa
     5941 aaatcctcag gcattagccc gtgcgctggt gcaaccgttg ctgttggcgt tgggggctgg
     6001 ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg gctctgcata ttgttggcgg
     6061 tagtgaagca gtcctggaac aggcgcggcg ctttcttgaa atccgctggt taagcgcacc
     6121 ggcgtcgctg gcgaatctgg tattactcgg ttggttactc ggcgtgcaat atgcccgtgc
     6181 gccagtaatt ttgttagtgg tcggcaatat cctcaacatt gtgctggatg tctggctagt
     6241 gatggggctg catatgaacg tgcagggcgc ggctctggcg acggttattg cggaatatgc
     6301 aacattgctg attggtctgc taatggtgcg taaaatcctc aaactacgcg gaatttccgg
     6361 cgaaatgtcg aaaactgcct ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga
     6421 tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc gcgatcaccg tacttggcgc
     6481 gcgactgggg agtgacatta tcgctgtaaa cgcggttctg atgacgctac tcacctttac
     6541 cgcctatgcg ctggatggtt ttgcctacgc ggttgaagca cattccggtc aggcatacgg
     6601 tgcgcgcgac ggtagccagt tgctggatgt ctggcgggca gcgtgccgcc agtcggggat
     6661 cgtagcgtta ctattttcgg tggtttattt gctggcaggg gaacacatca ttgcgttgct
     6721 gacgtcgtta acccagattc agcagctggc tgaccgctat cttatctggc aggtgatttt
     6781 gccgttggtt ggcgtctggt gttatctgct ggacggcatg tttataggcg caacgcgtgc
     6841 cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt tttgcgctga cgctccttac
     6901 gctgccgtgg ctgggtaatc atggtttgtg gctggcatta accgtctttc tggcgttgcg
     6961 cgggctttct ctggcggcta tctggcggcg tcactggcgt aacggtacct ggtttgccgc
     7021 aacgtgacgg ttaaaaattc tgaataaatt atcctaagcc aaattgctga ctacacttaa
     7081 tctcacgttc agaagaaaag tgaacgtact ctcattcaca acctaacgat gaggtcttga
     7141 ttatgaataa agatgaagcc ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc
     7201 aatggggcaa actgaccgat gatgatatga cgatcattga aggtaaacgt gatcaactgg
     7261 tcggtaaaat ccaggaacgt tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg
     7321 attgggaaac ccgcaatgaa tatcgctggt aattaatccc tcctgcccga cgtgtacaag
     7381 gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac agagtgatca tgctggcact
     7441 gttcaggatg acgacacgct tccacttcca cgcatgccgc acagagccca tgtgcttcaa
     7501 tcacattatg ccgcagggca aaccccattt ttgccgccag cgtatgcata atgtcttcca
     7561 cgccttctgc acactcttct ttcaccgcgc cgcagcgatc gcatataaac atggctgacg
     7621 tatgcgtggg ctggtcgaac agatgacaga gcacataact gttggtggat tccaccttat
     7681 gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata aaccgttggc ggcttggctt
     7741 gcggttcagc ttcgcgcagt aaatcaagca gatcataagc gctgatagcg ccatcctgca
     7801 gactcatcag gcgcaacact tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc
     7861 agattttttc agcctgcgct aataactcct gcgttgtggt cttttccatc gggcacctca
     7921 aagtgggggt taagaatgtc cttactttac catgttccag gaaaaacacc gagaaccctt
     7981 attgttgccg taatgttgat tttctgtttt gtaggtaagg tgttatgttg ccttgtcgta
     8041 ccattatcaa cacgataata attaataatt catttttaaa taaattgctt caagttaact
     8101 cgctatttat ttaactgggt ttaaacgcat acctgtagtt cagaaattat gagcgatttt
     8161 tgatagtgac cagatggcaa aagaattata aggaggacac ggtacgacaa aaaaggtaga
     8221 tgaatcatgt tgaagataat acccggtgca actggatact ttaataaaac actcaattca
     8281 aatcaattcg ataatgaaga tgcaatcaaa gataaattag ataatagagg tt
//
LOCUS       DAZOZY010000175         8211 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZOZY010000175 DAZOZY010000000
VERSION     DAZOZY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8211
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.176"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(349..1545)
                     /gene="hemY"
                     /locus_tag="ACHURQ_003367"
     CDS             complement(349..1545)
                     /gene="hemY"
                     /locus_tag="ACHURQ_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR0825079.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1548..2795)
                     /gene="hemX"
                     /locus_tag="ACHURQ_003368"
     CDS             complement(1548..2795)
                     /gene="hemX"
                     /locus_tag="ACHURQ_003368"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR0825080.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPAPQGDTPAAAPQGE"
     gene            complement(2817..3557)
                     /gene="hemD"
                     /locus_tag="ACHURQ_003369"
     CDS             complement(2817..3557)
                     /gene="hemD"
                     /locus_tag="ACHURQ_003369"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR0825081.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3554..4495)
                     /gene="hemC"
                     /locus_tag="ACHURQ_003370"
     CDS             complement(3554..4495)
                     /gene="hemC"
                     /locus_tag="ACHURQ_003370"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR0825082.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4882..7428
                     /gene="cyaA"
                     /locus_tag="ACHURQ_003371"
     CDS             4882..7428
                     /gene="cyaA"
                     /locus_tag="ACHURQ_003371"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR0825083.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7468..7788)
                     /gene="cyaY"
                     /locus_tag="ACHURQ_003372"
     CDS             complement(7468..7788)
                     /gene="cyaY"
                     /locus_tag="ACHURQ_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR0825084.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7950..8092)
                     /locus_tag="ACHURQ_003373"
                     /pseudo
     CDS             complement(7950..8092)
                     /locus_tag="ACHURQ_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1824 a         2172 c         2146 g         2069 t
ORIGIN      
        1 aaatattcac tcaggaagca aagaggatta cagaattatc tcataacaag tgttaaggga
       61 tgttatttcc cgattctctg tggcataata aacgggtaga tgctcattcc atctcttatg
      121 ttcgccttag tgcctcataa actccggaat gacgcagagc cgtttacggt gcttatcgtc
      181 cactgacaga tgtcgcttat gcctcatcag acaccatgga cacaacgttg agtgaagcac
      241 ccactgttgt catacagacc tgttttaacg cctgctccgt aataagagca ggcgtttttt
      301 tatgcatcag aaaggtcccg gaggtgcttg cctccgggtg agaaggaact actgtggtgg
      361 gttgttctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcagctt cttccggctt
      421 gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg gacgttgttt
      481 cagcgctgcg cggaaggcga gcgatgcttc ctgccattct ccgtgcttca tcagtgactg
      541 gcccagtgtg ctccacaaca gcgggcgatc gccgacgttt ttgatctgct ggcgcagcac
      601 tttttccagc tgttccggat tgttggtttt cagtcgagga atcggcagca gtaggcgatc
      661 gtcgtactgg cgtttcaggc catcgatgat aatttgctgg gcagtatcat gatcgtcaca
      721 ttcaataaga tgttccgcca ttgccacctg caacgctacc tgatgacgcg ttttccggct
      781 ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg cctgatccat
      841 caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac caacatgggc
      901 tttcgccatt gatgggatga tatccagcag agaactccat gcacctgtgc ggatatacgc
      961 ctgttccgcc agacgtaata cttccggatg gcgtggcgta acttccagca gcttatccac
     1021 gccgtggcgt gcagcatggt tttcattacg ggccagttgc agacgtacac gggtgatttc
     1081 taccggaatg gtgtcattgc cggccagctc tgctgcgcgt tccagatgtt ggttggcgcg
     1141 tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt agatagttca ccaccggttg
     1201 ttccgcgtga tcggcatttt tcgccatcag cttttcaact tgctgataat cgccttccgc
     1261 cagtttcagc agcgcctgtt cggtctgctt acgggcacgg cgacgcttac gtccgacaaa
     1321 ccacccacgg gtgtgtgcgc ctgtgcggaa gatccgccgc agtagccact caatggcaaa
     1381 cagcactacc atcgccagaa tcaaaataat cgccaggccc gtgacgctgg tttcgatatt
     1441 gtagttgtca gtctggatca gcacgtaacc ctgatgaccg gcaatcatcg ggccaaccac
     1501 tatccccgca atcagcaaaa caaagagtaa taacactttt agcatggtta ttctccttgc
     1561 ggcgcggctg ccggagtatc accttgcggt gcaggtgcag gtgcaggtgc aggtgcaggt
     1621 gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggttt agcttccgtt
     1681 gtccccgctg ccggttgtgc cagcaggtta cgcacgcggg tctgcatcag tttttccagc
     1741 atcgcctggc tttgcagggt ttccggaaga tccatcgaga tattttgctg gcttaactgg
     1801 tccacctcgt cgaggaacgc tttggtggtg gcatcatcag tatcgtagta agcacgtacc
     1861 caggtggaga cgttctccag cgcctggcga taagtctctt cctgatgacg cggtacagct
     1921 tgtgctgcga ccagcaggcg agagcgaata ttttcgcgca gatagatatc ctgatttggc
     1981 gctaacagcg gtacggcggt gtcatcacga cggcgaatcg taatgaagtt gtccataaag
     2041 ttctgccagc ttttttgcag attgatacgc cattcgctga tggaactgga aagctcttca
     2101 ccgtctgaat ccatcggcga accatcgctg tcattatcgg ccagacgcag gttatctacc
     2161 tgatttgaaa gctgattaag cttaaggatg atgccgtcat aatccacctg cgatactgca
     2221 gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg taatcagact cgggtcattc
     2281 atatccgcca ggctggcgtc tgcacttttc agcaacgctg cagcggtcgt gacgtcctga
     2341 tcgctccaca gcttccgtcc ggcgagtttc accagaaaat cggcctgggc cagcagccag
     2401 gttttagcat cgctgccgga aatggtggcg accttttgct ggacttcatc cagctgtttt
     2461 gccagcgttt cttgctgacg attcgcctgt tcaagctgcg tagcctgttg cttaataatg
     2521 ccttccagtt cggctttttg gctctcctgg gctttttgca atgccgtcag ttggttagcc
     2581 agggcatcgc tggtggcggt ctgattgacg gcctgttgtt taccccagcc atacaaaccg
     2641 acgcccgccg ccagagcaat agcgatagcc gccgcgctga gaatcaatgc ggtattgttc
     2701 ttactctttt tttctgttgc gacaggttgt gacgtggtgt ccacggcctc cctagtctct
     2761 tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg cttcctgtta tgagagttat
     2821 tgtaatgccc gtaaaagcgc atcgttggca gcgttatcgg cgaccttaat gtcttgccag
     2881 cccagttccc gggcgagttt cgccaaacgc tcactgacga ccaatagtcg acagtgtagt
     2941 aaccagtgct cacgatacca ttgtgggatc agcgaccaga gttgctgcaa catttcaccg
     3001 ctggtaacaa cgaccgtcgt cacctcgcgg gattgccagc gcatcgcttc ttctgcacca
     3061 tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag tgacctcagc accgcgcgcc
     3121 gtcagggtat ccccaattag ctcacgaccg ccattgccac gtaatatcag cgcacgtttg
     3181 cccgcaatat tttgtaattc aggtaattgt agcaagactt cgctgatttc ccgatcctgc
     3241 gggtagagaa tcttctgtcc gcttacggta tgtagtgcca gtgcggtggt gcgtccaatg
     3301 gcgaaataat taggtagtcg gggccattta cgatcttgct gatgtagctg tgattgggca
     3361 aaagcaaccg cgtgttgcga gagggcaaac aacagatcgc tctcccccag cgctgccagt
     3421 tgatcagcaa gttgcggtaa ttgttgaccc ggagaaaact caatcagcgg aaaatgccag
     3481 gccacctgcc ccagtgtgcg cagacggctc actaactctt ctccagcggg agacgggcgg
     3541 gtgacaagga tactcatgcc ggggcgtctc cgttatagac ttcagcgagg atctcgcgcg
     3601 cgccgttatt cagtagctct tctgccagcg aaatccccat ttgtttggca ttttgcggcg
     3661 caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg cgcgccgacc agcgcacgca
     3721 gccagatttc gccatcaata agctcggcgt agctaccaat tggcacctga catccgcctt
     3781 cgagacgggt attcatggcg cgttctgcgg taacgcgcag tgcagtttcg tggtgattca
     3841 gcgcggcaag cagctcgcga gtgcgcgtat catcaaggcg gcattcaata ccgactgcac
     3901 cttgtcctac cgccggaaga gaaatctcgg gtggtaacgc ggcgcgaatg cgggactcca
     3961 gacctaaacg ttttagtccg gctacggcaa gaatgatggc atcgtattcg ccgttatcta
     4021 gtttgctcag gcgagtgccg acgttgccgc gcagggagcg gataatcaga tccgggcggc
     4081 gttcagccag ttggcactgg cgacgtaaac tggacgtccc gacgatactg cctgctggta
     4141 acgcatccag actgtcatag ttattggaca caaaggcatc gcgaggatct tcacgctcac
     4201 aaatagtgac cagtcccaga ccttgcggga attcaaccgg cacatctttc attgaatgta
     4261 cggcgatatc ggcgcgattt tcgaggagcg cgacttccag ctcttttaca aataagcctt
     4321 ttccgcctac tttcgccagc ggcgtatcaa gaatcacatc gccgcgcgtc accatcggta
     4381 ccagttcaac gaccaggccc ggatggctcg ccatcaactt gtctttgaca tagtgtgcct
     4441 gccagagtgc aagtgggctt tggcgtgtgg caattcttaa aacattgtct aacatgcttg
     4501 ttaccgtcat tatcatccgt ggtccatcct aacatccttg ccagagtgat gtcagtgttg
     4561 tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt ctggcagtgg atcctgacag
     4621 acgtttcacg ctgtcgtaat aaggaattta cagagaataa acggtgctac acttgtatgt
     4681 agcgcatctt tctttacggt caatcagcaa ggtgttaaat tgatcacgtt ttagaccatt
     4741 ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg taacggttac ctttgacata
     4801 cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc ggaatcacag tcatgacggg
     4861 tagcaaatca ggcgatacgt cttgtacctc tatattgaga ctctgaaaca gagactggat
     4921 gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta tggggcctgc attccaacag
     4981 gtctacagtc tactgccgac attgttgcac tatcaccatc cgctaatgcc gggttacctt
     5041 gatggtaacg ttcccaaagg catttgcctt tacacgcctg atgaaactca acgccactac
     5101 ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg atccaccgaa aggtgagctt
     5161 ccaattactg gtgtatacac catgggcagc acctcgtccg tagggcaaag ttgttcctct
     5221 gacctggata tctgggtctg tcatcaatcc tggctcgata gcgaagagcg ccagttacta
     5281 caacgtaaat gcagcctgct ggaaagctgg gccgcctcgc tgggtgtgga agtcagcttc
     5341 ttcctgattg atgaaaaccg ctttcgtcat aatgaaagcg gcagcctggg gggcgaagat
     5401 tgtggctcca cccagcatat actgctgctt gacgaatttt atcgtaccgc cgtgcgtctc
     5461 gccggtaagc gtattctgtg gaatatggtg ccgtgcgacg aagaagagca ttacgacgac
     5521 tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa atgaatggct ggatctcggt
     5581 ggcttaagct cgctttctgc tgaagagtac tttggtgcca gcctttggca gctctacaag
     5641 agtatcgatt ccccatacaa agcggtgctg aaaacactgc tgctggaagc ctattcctgg
     5701 gaatacccga acccacgtct gctggcgaaa gatatcaaac agcgtttgca cgacggcgag
     5761 attgtatcgt ttggcctcga tccatactgc atgatgctgg agcgtgttac tgaatacctg
     5821 acggcgattg aagatttcac ccgtctggat ttagtacgtc gctgcttcta tttaaaagtg
     5881 tgcgaaaaac tcagccgtga acgcgcctgt gtaggctggc gtcgcgcagt gttgagccag
     5941 ttagtgagcg agtggggttg ggacgaagct cgtctggcaa tgctcgataa ccgtgctaac
     6001 tggaagattg atcaggtgcg tgaggcgcac aacgagttgc tcgacgcgat gatgcagagc
     6061 taccgtaatc tgatccgctt tgcgcgtcgc aataacctta gcgtctccgc cagtccgcag
     6121 gatatcggcg tgctgacgcg taagctgtat gccgcgtttg aagcattacc aggtaaagtg
     6181 acgctggtaa acccgcagat ttcacccgat ctctcggaac cgaatctgac ctttatttat
     6241 gtgccgccgg gccgggctaa ccgttcaggt tggtatctgt ataaccgcgc gccaaatatt
     6301 gagtcgatca tcagccatca gccgctggaa tataaccgtt acctgaataa actggtggcg
     6361 tgggcatggt ttaacggtct gctgacctcg cgcacccgtt tgtatattaa aggtaacggc
     6421 attgtcgatt tgcctaagtt gcaggagatg gtcgccgacg tgtcgcacca tttcccgctg
     6481 cgcttacctg caccgacacc gaaggcgctc tacagcccgt gtgagatccg ccatctggcg
     6541 attatcgtta acctggaata tgacccgaca gcggcgttcc gcaatcaggt ggtgcatttt
     6601 gatttccgta agctggatgt cttcagcttt ggcgagaatc aaaattgcct ggtaggtagc
     6661 gttgacctgc tgtaccgcaa ctcgtggaac gaagtgcgta cgctgcactt caacggcgag
     6721 caatcgatga tcgaagccct gaaaactatt ctcggcaaaa tgcatcagga cgccgcaccg
     6781 ccagatagcg tggaagtctt ctgttatagc cagcatctgc gcgggttaat ccgtactcgc
     6841 gtgcagcaac tggtttctga gtgtattgaa ctgcgccttt ccagcacccg ccaggaaacc
     6901 gggcgtttca aggcgctgcg cgtttctggt caaacctggg gcttgttctt cgaacgcctg
     6961 aatgtatcgg tacagaagct ggagaacgcc atcgagtttt atggcgctat ttcgcataac
     7021 aaactgcacg gcctgtcagt gcaggttgaa accaatcacg tcaaattacc ggcggtggtg
     7081 gacggctttg ctagtgaagg gatcatccag ttcttcttcg aagaaacgca agacgagaat
     7141 ggctttaata tctacattct tgacgaaagc aaccgggttg aggtgtatca ccactgcgaa
     7201 ggcagcaaag aggaactggt gcgtgacgtc agtcgcttct actcgtcatc gcacgaccgc
     7261 ttcacctacg gctcaagctt catcaacttc aacctgccgc agttctatca gattgtgaag
     7321 gttgatggtc gtgaacaggt gattccgttc cgcacaaaat ctatcggtaa catgccgcct
     7381 gccaatcagg atcacgatac gccgctatta cagcaatatt tttcgtgatg aacgtgccgg
     7441 aaagcgaggc ttatccggca tgcaatctta gcggaaactg actgtttcac ccgcctgctg
     7501 cgtcgccgcc tgttccagca aatcccagaa ggtttcgccg ctgcgatcgc aaatccactc
     7561 atcgcctttc agatcaaaat ggtagccgcc ttgtttggtt gccagccata cctggtgcag
     7621 cggttcctgg cggttgataa tgattttgct gccgttttca aaggtaatgg tcagcacgcc
     7681 gccgttgatt tcgcagtcga tatcgctgtc gccatcccag tcgtccaggc gttcttcaat
     7741 ggtcagccag agttgatcag ccaggcgatg aaattcactg tcgttcattg ttgtatcctg
     7801 tttttaagtg atggcggcag tatagcggca tggggtcagg gcttcaaagt ttgcacctct
     7861 gcggctgcgt tccggcacga ttcatccgtc accggaataa tgatgtctct gtgtagcgaa
     7921 agatttgtct cttcattagg gcgcagttac accacgcctt tccctgtttc tggtaaacat
     7981 tatgatcagg tttaccgagc gagcatcctc acgctgacgg aactaaaaaa gacaacaaac
     8041 aaaccacatt gcgatagtgc atagagccat cctggcgcga ggtgccgatc acgaaactac
     8101 cagcaaaaca taaatcccca cgagtaagcg ttatactcgc agcatttcct cacttttcag
     8161 acttcataaa gagtcgctaa acgcttgctt ttacgtcttc tcctgcgatg a
//
LOCUS       DAZOZY010000176         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZOZY010000176 DAZOZY010000000
VERSION     DAZOZY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.177"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHURQ_003374"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHURQ_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR0825085.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHURQ_003375"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHURQ_003375"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR0825086.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHURQ_003376"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHURQ_003376"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR0825087.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHURQ_003377"
     CDS             6679..>8182
                     /locus_tag="ACHURQ_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR0825088.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZOZY010000177         8175 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZOZY010000177 DAZOZY010000000
VERSION     DAZOZY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8175
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.178"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHURQ_003378"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHURQ_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR0825089.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHURQ_003379"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHURQ_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR0825090.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHURQ_003380"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHURQ_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR0825091.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHURQ_003381"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHURQ_003381"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR0825092.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHURQ_003382"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHURQ_003382"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR0825093.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHURQ_003383"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHURQ_003383"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR0825094.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHURQ_003384"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHURQ_003384"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR0825095.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHURQ_003385"
     CDS             5315..6439
                     /locus_tag="ACHURQ_003385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR0825096.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHURQ_003386"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHURQ_003386"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR0825097.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>8175
                     /gene="waaO"
                     /locus_tag="ACHURQ_003387"
                     /gene_synonym="rfaI"
     CDS             7245..>8175
                     /gene="waaO"
                     /locus_tag="ACHURQ_003387"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR0825098.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARY"
BASE COUNT         2098 a         1883 c         2068 g         2126 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttatt
//
LOCUS       DAZOZY010000178         8157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZOZY010000178 DAZOZY010000000
VERSION     DAZOZY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.179"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHURQ_003388"
     CDS             <1..254
                     /locus_tag="ACHURQ_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0825099.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHURQ_003389"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHURQ_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR0825100.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHURQ_003390"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHURQ_003390"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR0825101.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHURQ_003391"
     CDS             complement(2905..3180)
                     /locus_tag="ACHURQ_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR0825102.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHURQ_003392"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHURQ_003392"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR0825103.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHURQ_003393"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHURQ_003393"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR0825104.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHURQ_003394"
     CDS             5140..5466
                     /locus_tag="ACHURQ_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR0825105.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHURQ_003395"
     CDS             5650..6369
                     /locus_tag="ACHURQ_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR0825106.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHURQ_003396"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHURQ_003396"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR0825107.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="ACHURQ_003397"
     CDS             7708..>8157
                     /locus_tag="ACHURQ_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0825108.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2087 a         1988 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAZOZY010000179         8081 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZOZY010000179 DAZOZY010000000
VERSION     DAZOZY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.180"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..171
                     /locus_tag="ACHURQ_003398"
     CDS             <1..171
                     /locus_tag="ACHURQ_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825109.1"
                     /translation="SSSEEFLFDNSLEKDENSSDNCFEVEKYNAYEAYNSENLYFTRE
                     EYTYDYDLLNAI"
     gene            complement(559..935)
                     /gene="rnpB"
                     /locus_tag="ACHURQ_003399"
     ncRNA           complement(559..935)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHURQ_003399"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(968..2113)
                     /gene="garK"
                     /locus_tag="ACHURQ_003400"
     CDS             complement(968..2113)
                     /gene="garK"
                     /locus_tag="ACHURQ_003400"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR0825110.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2210..3100)
                     /gene="garR"
                     /locus_tag="ACHURQ_003401"
     CDS             complement(2210..3100)
                     /gene="garR"
                     /locus_tag="ACHURQ_003401"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR0825111.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3130..3900)
                     /gene="garL"
                     /locus_tag="ACHURQ_003402"
     CDS             complement(3130..3900)
                     /gene="garL"
                     /locus_tag="ACHURQ_003402"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR0825112.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3916..5250)
                     /gene="garP"
                     /locus_tag="ACHURQ_003403"
     CDS             complement(3916..5250)
                     /gene="garP"
                     /locus_tag="ACHURQ_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR0825113.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5625..7196
                     /gene="garD"
                     /locus_tag="ACHURQ_003404"
     CDS             5625..7196
                     /gene="garD"
                     /locus_tag="ACHURQ_003404"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR0825114.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7345..7750
                     /locus_tag="ACHURQ_003405"
                     /pseudo
     CDS             7345..7750
                     /locus_tag="ACHURQ_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
     gene            7750..>8081
                     /gene="yhaV"
                     /locus_tag="ACHURQ_003406"
     CDS             7750..>8081
                     /gene="yhaV"
                     /locus_tag="ACHURQ_003406"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR0825115.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT         2073 a         2141 c         1940 g         1927 t
ORIGIN      
        1 tctagctcag aggaattcct ttttgataat agcctcgaga aagacgaaaa ttcttcggac
       61 aattgctttg aggtcgaaaa atataacgca tatgaagcat ataactcaga gaatctatat
      121 tttaccagag aggaatatac gtatgattac gaccttttaa atgcaatata gacataataa
      181 cttcttacaa aatctataat taataccgta gatattcatt catcttaccc tgtgcaccaa
      241 aagcaaaaac tcactgacgc gggtttgaaa ctgatggcaa ccgcaaaaat gcctgatgcg
      301 ctacgcttat caggcctacg ccatctctac aatatattga atttgcgtgc ttttgtaggc
      361 aggataaggc gttcacgcag catccggcat gaataaagcg cactcaacaa tctgaaaccc
      421 gccggaacgg tttattacgt acatcaggtg aaactgaccg ataagccgct ttcttttggg
      481 tatagtgtcg tggacagtca ttcatctttc tgcccctcca aaagcaaaaa cccgccgaag
      541 cgggttttta cgtaaatcag gtgaaactga ccgataagcc gggttctgtc gtggacagtc
      601 attcatctag gccagcaatc gctcactggc tcaagcagcc tacccgggtt cagtacgggc
      661 cgtaccttat gaacccctat ttggccttgc tccgggtgga gtttaccgtg ccacggactg
      721 ttaccagccg cgcggtgcgc tcttaccgca ccctttcacc cttacctgat cccgcttgcg
      781 cgggccatcg gcggtttgct ctctgttgca ctggtcgtgg gtttcccccc caggcgttac
      841 ctggcaccct gccctatgga gcccggactt tcctcccctc cgcccgtctc ccccgaagag
      901 gacgacgacg aagcggcgac tgtctggtca gcttcggcgc gcagtataga gggtttgcgc
      961 gcccttgtca ccccgcgttg cgcattccaa tcgccagtgt cgcggcgata ttacgtgaag
     1021 cacggcagat attgtcataa gccccgcgga atgcttcgtc caacgtacct atgctggtca
     1081 atacgctgaa gaccgcatcg atgccatgct gatgtacaac gccaacatca tcggtcaggc
     1141 tacccgcaat gccaatcacc ggtttatggt acttcttcgc cacgtttgca acgccaatcg
     1201 gtaccttgcc gtgaatactc tggctgtcaa tacgcccttc accggtgatc accagcgtac
     1261 aatcgtgaat atgttcttcc agattcagcg ccgtagtgac gatttcaata ccacttttca
     1321 gttccgcacc aagaaacgcc atcagcgccg cgcccatacc gcccgcagct cctgcgccgg
     1381 ggacatcttt cacatcaaca tgcagcgctt ttttaatgac atcggcatag tgagagaggt
     1441 tattgtccag ctcaacaatc atcgcttcac tggctccctt ttgtgggcca aagatgcgcg
     1501 atgcgccgtt atcgcccacc agcggattgg tgacatcaca agcgacgcga atgacgcaat
     1561 cttttaagcg cggatcgagg ccggaaatat caatatcatt cagagtatta agactaccgc
     1621 cgccaaaacc aatttcattg ccgttggcgt cgcataattt cgcccccagc gcctgtacca
     1681 tgcctgcgcc gccatcattt gtagcgctgc cgccaatgcc gataataatg tttgttgcac
     1741 tgctctccag cgcctgcagg attaactcgc ctgtgccgcg tgaagtggtt acgagtggat
     1801 cgcgtttttc cgcaggtacc agctccagcc cactggccgc cgccatttca ataaacgcgg
     1861 ttttgccatc gccggagatc ccccaactgg cattcacttt ctcgcccagc ggccctgtaa
     1921 cccaggcgtg acgttcagcc ccctgggtgg ctgcaatcat cgcttccacc gttccttcac
     1981 caccgtcggc aaccggaaca gaaacatact gtgcatcagg aaaaatttcc cgaaatcctt
     2041 tttctatcgc ctgcgcaacc tcgctggcag ataaactttc tttataagag tctggggcga
     2101 ttacgatttt catacctatg cctgttacca catgacgccg gagggcgttt ctcttattcg
     2161 gcctagattc caggcccgga ttgcaataca ccatccgggc acgacgtcat taacgagtaa
     2221 cttcgacttt cgccagtttt tcgtagtagc acgccagggc gctatgatcc gccgttccta
     2281 aaccatctgc acgcagtgcc tgcatcatct ccataaccgc agctgtgagc agcagttgtg
     2341 cgccgacgcc gtgagaagta tccagcgcat tcgccagatc cttaatatgc agatcaatgc
     2401 ggaagcccgg cttgaagttg cggtccatca ccatcggcgc tttggcatcc agcacggtac
     2461 tgcccgccag cccaccgcga attgcctgat aaaccaggtc cgggttaacg cccgctttgg
     2521 ttgccagcgt taacgcttct gacatcgcag caatattcag cgccacaatg acctgatttg
     2581 ccagtttggt gacgttacct gcaccgattt ccccggtatg caccacggaa cccgccatcg
     2641 ctttcatcaa atcatagtat ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca
     2701 gcgtaccgtc gatggctttc ggttcaccgc cgctcaccgg agcatccagc atatcaatgc
     2761 ctttcgcttt cagcgcttcg ctgatttcac ggcttgccag cggtgcgata gaactcatat
     2821 cgatcaatac cgtacctggc ttcgcgcctt caataatgcc attctcaccc agcgccacct
     2881 ctttcacatg aggggagttt ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg
     2941 ctttagccgt agacgctgtt tctgcacctg cagcaatcac gtcagcaata gcttctgggt
     3001 tacggtcagc aaccaccagc gagtaacctg ctttcagaag gtttttactc attggtttac
     3061 ccataatccc caggccaata aaaccaactt tcatagtcat atcaaccatc tctcttgttg
     3121 cggtggtggt tattttttaa aggtatcagc cagtttctga gtggcagagc ggaagacgcc
     3181 aagatcgctg ccgacagcca caaacgtcgc gccccattcc agataacgac gcgcatcggc
     3241 ttcgaccggc gcgagaatac cgctgggttt gccgtgcgcg ctggcacggt taaaaatatg
     3301 ctgaattgct ttttgtacat ccggatgcga ggcattgccg agatggccca atgccgcagc
     3361 cagatcgctg gggccgacga agataccgtc tacaccttcg gtagcggcaa tagcatcgac
     3421 gttatctacg ccctgctgac tttctatctg gaccagaata gtaatgttct tgttcgactg
     3481 agcgaaataa tccgccacgg tgccaaacat attggcgcgg tgagaaacgg agacgccgcg
     3541 aatgccttcc ggcgggtaac gagttgatgc caccgcctgc tctgcttcct cttttgtttc
     3601 tacaaaagga atcaggaagt tatagaaacc gatatccaga agacgcttaa taattaccgg
     3661 ctcgttggtc ggcactcgca ctactggcgc gctggcgctg cctttcaggg ccattaactg
     3721 cggaataaac gtggaaatat cgtttggcgc atgttcgcca tccagcacca gccagtcaaa
     3781 cccagccaaa ccaagaactt cagtgctaat cgggttagag agtgctgacc agcaaccaat
     3841 ttgtacctgt ttcgcagcca gtgcggcttt gaatttattc gggaaaacat cgttattcat
     3901 cgcttatacc tttgcttatt tctgcaattc catacgttta atgtcgccaa ctacgaagag
     3961 gtagcagacc atcgccatca gcgctgaaca tcccacgaaa accagtgctg cattgaagga
     4021 gtgcagttca cttaccaggt agccaatcac cagtggagtg acaatggagg caacattgcc
     4081 aaagacgtta aagacgccgc cacagaggcc aacaatctct ttcggcgcgg tgtcagaaat
     4141 caccggccag cccagagcac caaatccttt gccaaaaaaa gccagcgcca tcagcatgac
     4201 caccagcgtg gtgttgttgg tgtagttaca taagatgatg gtggaaacca gcaacattcc
     4261 cagcacaatc ggtagcttac gtgccagggt cagggataaa ccgcgtttga tcagataatc
     4321 cgagaagaca cctcccagca cgccgcccgc aaaaccacac agtgccggaa tcgaggcgac
     4381 cagacccact ttcagaatcg acatgccttt ttcctgcacc agataaatcg ggaaccaggt
     4441 gaggaagaac caggtgatgg tgttgataaa atattgtccg aaaaatacgc ccaacatcat
     4501 gcggttagag agcaattgct tgatgtaatg cagtttggga ccgcttgctg ccgcgctgcc
     4561 cggctttttg tggtccatat cgaccaccgc gccattttca gagataaact tcagctcttc
     4621 cgcagacata cgtgggtgat ctgtcgggtt atgaatcaac ttgatccaca gcgccgtcag
     4681 cacaaaacca atccccccca taacggtgaa gacgtgctcc cagccccaag cgaaagtcag
     4741 ccagccaagc agcggcgaaa agagcgccag cgagaaatat tgcgccgagt taaagatggc
     4801 ggaggcagta ccacgttctt tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg
     4861 gaatgatggc gcttccgaga accccagcat aaagcgcata aagaacatgg agatccctgc
     4921 ccaggccagc gggaacatat caacaaagcc ttgcaggaag gtgaacagcg accagaaaaa
     4981 gaggctgtag gtgtaaactt ttttcgagcc aaacttatca agcaaccagc cgccggggat
     5041 ttgcatcagc aagtaggccc agccaaaagc agagaagatg taacccatcg aaaccgcact
     5101 taactgcaac tcttttgcca cttcggtacc agcaatagac agcgttgcac gatcggcgta
     5161 gttaacggcg gtaacaataa aaataatcag taatattaaa tagctggtat gcacgccttt
     5221 cttttttacg tcaacggtgt ccagaatcat tttatttacc tcgggtactt atgctgattt
     5281 ttattattat ggggaaggtg ttatttatga gttttattta tgccgttacg acaatgaact
     5341 cgggaattag tataagcagc gggagaataa taatcattgt gcaaatgcta atttaatcag
     5401 tactatttaa atattatttt gagcatatgc acataaggtt gcgcgctaaa gcacagattt
     5461 gcgctttacc ttaccgggcg gcactgcaat ccctgaaatg attgacattg atcacatttc
     5521 tgcgtttaaa ctcctgacat tcttatttca cccaatgaag tcatttattt ttaaatgaga
     5581 ccagatcctc attttaataa cccctggctg gagaatattg cacaatggcc aacatcgaaa
     5641 tcagacaaga aacgccaact gcgttttata taaaagttca cgacacagat aatgtggcaa
     5701 ttattgttaa tgataatggc ctgaaagcag gaacgcgttt tccggatggg ctggaattaa
     5761 ttgaacatat tccccagggg cataaagtcg cattgctgga cattccagct aatggtgaaa
     5821 ttattcgtta tggcgaagtg attggttacg ccgtgcgcgc aatcccacgc ggaagctgga
     5881 tcgacgaatc aatggttgta ctaccggaag cgccgccgtt acacacgctg ccactggcaa
     5941 ccaaagtccc ggccccctta ccgccgctgg aaggatacac ctttgagggc tatcgcaatg
     6001 ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg
     6061 caggcgtggt ggactatgta gtaaaaatca ttgaacgcga tctgctaccg aaatacccga
     6121 acgtcgatgg cgtggtgggg ctgaatcatt tgtacggctg tggcgtggcg attaacgcac
     6181 cggcggcagt tgtgcctatt cgtaccatcc acaatatttc gctgaaccct aactttggcg
     6241 gcgaagtaat ggtgattggt ctgggttgtg aaaagttgca gcctgagcgc ctgctggtcg
     6301 gcacggatga tgtgcaagct attccagtag aaagcgccag cattgtcagt ttgcaggatg
     6361 aaaagcatgt cggctttcag tccatggtcg aggatatttt gcaggtcgcc gaacgccatc
     6421 tacaaaaact gaatcaacgg cagcgagaaa cctgcccggc ttcagaactg gtcgttggtg
     6481 tgcagtgcgg tggcagcgat gcgttttctg gtgtaacggc aaacccggcg gttggctatg
     6541 cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt ttcagaagta acggaagtgc
     6601 gtgacgcgat ccatctgctg acaccacgcg cagtgaacga agaggtcggc aaacggctgc
     6661 tggaggagat ggagtggtac gataactatc tcaatatggg aaaaaccgac cgcagcgcca
     6721 acccttcgcc gggcaacaag aaaggcggtc tggcaaatgt ggtggagaag gcactcggct
     6781 ccattgctaa atcgggtaaa agtgcaattg ttgaagtgct gtcgcccggt caacgcccga
     6841 ctaaacgcgg attaatttac gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac
     6901 aggtggcttc gggtatcacc gtacaagtgt ttacgaccgg ccgtggtacg ccgtacggcc
     6961 tgatggcggt acccgtaatt aaaatggcaa cccgcaccga gctggcgaac cgctggtttg
     7021 atttaatgga tattaacgcg ggcactatcg ctaccggcga agagagcatt gaagaggtgg
     7081 gctggaagtt gttccacttt attctcgacg tcgccagcgg gaagaagaaa accttctcgg
     7141 atcaatgggg gctgcataac cagctggcgg tgtttaaccc ggcaccggtg acctgatttc
     7201 ttttggaacg tccctcgcaa aacatggcct tagtgccatg tttttattgt ttaaagcccc
     7261 cacgtccatt aataatgcat ttgcattacc ttagttcaag cttataattt gagcagaaaa
     7321 caggctgtaa aaggacaatg aatcatgccc gctaatgctc gctctcacgc tgtactgacc
     7381 actgaatcaa aggtcacgat acgcggacaa acaactatcc ccgcgccagt gcgtgaggcc
     7441 ttaaaactga agccaggcca ggacagcatt cattacgaaa ttctgcctgg tgggcaagta
     7501 tttatgtgcc gactgggaga tgaacaggag gatcatacta tgaatgcatt tttgcgtttt
     7561 ctggatacag atatccagaa caacccgcaa aaaactcgtc cattcaacat tcaacattca
     7621 acattcaaca ttcaacattc aacattcaac attcaacatt caacattcaa cattcaacat
     7681 tcaacaagga aagaaacttg tcgctggcat ggacgtcaac attgatgatg agattggcga
     7741 cgacgaataa tggattttcc acaaagggtt aatggttggg cgctatatgc tcatccctgt
     7801 tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga cattaaaggg aaaagatcct
     7861 gaaaattatc agagaaaagc cgccacaaag ttattggcgg tagtccataa agtgattgag
     7921 gagcatatca cggtcaatcc atcatcaccg gcattccgtc atggcaagtc gttaggctct
     7981 gggaaaaata aagactggtc acgggtaaaa tttggtgcag gtcgttatcg tctcttcttt
     8041 cgttatagtg aaaaagagaa agtcatcatt ctgggatgga t
//
LOCUS       DAZOZY010000180         8054 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZOZY010000180 DAZOZY010000000
VERSION     DAZOZY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8054
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.181"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            103..468
                     /locus_tag="ACHURQ_003407"
                     /pseudo
     CDS             103..468
                     /locus_tag="ACHURQ_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
     gene            complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="ACHURQ_003408"
     CDS             complement(525..2903)
                     /gene="ppsA"
                     /locus_tag="ACHURQ_003408"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR0825116.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3236..4069
                     /gene="ppsR"
                     /locus_tag="ACHURQ_003409"
     CDS             3236..4069
                     /gene="ppsR"
                     /locus_tag="ACHURQ_003409"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR0825117.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4226..5272
                     /gene="aroH"
                     /locus_tag="ACHURQ_003410"
     CDS             4226..5272
                     /gene="aroH"
                     /locus_tag="ACHURQ_003410"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR0825118.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5404..5595
                     /gene="hemP"
                     /locus_tag="ACHURQ_003411"
     CDS             5404..5595
                     /gene="hemP"
                     /locus_tag="ACHURQ_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR0825119.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5599..7035)
                     /gene="selO"
                     /locus_tag="ACHURQ_003412"
     CDS             complement(5599..7035)
                     /gene="selO"
                     /locus_tag="ACHURQ_003412"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR0825120.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="ACHURQ_003413"
     CDS             complement(7098..7811)
                     /gene="ydiV"
                     /locus_tag="ACHURQ_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR0825121.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2073 a         2113 c         1910 g         1958 t
ORIGIN      
        1 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
       61 aaacaccatc atacactaaa tcagtaagtt ggcagcatca ccaaaattac cggacgcaaa
      121 aaagatatta ttgtccgcgg cggcgaaaat attagcagcc gtgaagtaga agatatttta
      181 ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa tgcccgatga acgtttaggt
      241 gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc attcattatc gctggaagag
      301 gtggtggctt tttttagccg taaacgggtc gcaaaatata aatatcctga acatatcgtg
      361 gtaatcgaaa aactaccgcg tactgcctca ggtaaaatac aaaagttttt gttaagaaaa
      421 gatattatgc ggcggttaac tcaggatgtc tgtgaagaga ttgaataagt ttcatcttcg
      481 gggatcacat aaccccggcg actgaacgcc gccggggatt tattttattt cttcagttca
      541 gccaagctta accaggtttg caccacggtg tccgggttca gagacaggct atcgatcccc
      601 tcttccatca accatgcggc aaagtcttcg tggtcggacg gaccctgacc gcaaatcccg
      661 acatatttgc cctgtttctt cgcggcacgg atagccatcg acagcagtgc tttcaccgca
      721 tcgttgcgct catcgaacaa ttcagacacc acgccggagt cacggtccag accgagcgcc
      781 agctgcgtca tatcgtttga gccaattgag aagccgtcga aatattcgag gaactgctcg
      841 gccagcaagg cgttggacgg gatttcacac atcatgatga ttttcagccc gttctcacca
      901 cgcttcagcc cctgacgcgc cagttcctca accaccgctt tcgcctgatc tacggtacgc
      961 acgaacggga tcatgatctc aacgttggtc agccccatgt cgttgcgcac acgtttcact
     1021 gcttcgcact ccagcgcgaa gcagtcgcgg aagctgtcgg aaacatagcg tcccgcgcca
     1081 cggaagccga gcatcgggtt ctcttcatct ggctcgtaac gctcaccacc gaccaggttg
     1141 gcatattcgt tcgatttaaa atcagagaga cggacaatga cgcgcttcgg ataaaacgcg
     1201 gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt aaaattcacg cggagaatca
     1261 aaacctttca tcatctcgcg gatttcattt tgcaactgcg gttcctgatc gtcaaactca
     1321 agcagtgcgc gtgggtggac gccaatcata cggttgatga taaattccag acgcgcaagg
     1381 cccacgcctt cgttcggcag gcaggcgaag tcgaaagcac ggtccgggtt accgacgttc
     1441 atcatcactt tcaacggcag atccggcatc atttctacgc tggagctttt cacgctaaat
     1501 tccagcaact ccgcatagac gtaaccggta tcaccttcgg cacaagaaac agtgacgttc
     1561 tcaccgtctt tcatccgttc tgttgcatca ccacagccca ctaccgccgg aatgcccagt
     1621 tcacgagcga tgatcgccgc gtgacaggta cgaccgccac ggttggtgac gatggcagat
     1681 gctttcttca tgatcggttc ccagtccggg tcggtcatgt cagtgaccag cacgtcacca
     1741 ggttcgatgc ggttcatttc gctgatctca tggatgactt tcaccggacc cgcgccgatg
     1801 cgatgaccga tagcacggcc ttcggcgata atcttaccct gtgaatgcag cgtataacgc
     1861 tccatgacct gaccgcgtga gcgcacggtt tccggacgcg cctgcacaat gaacagtttg
     1921 ccagtgtggc catctttcgc ccactcaata tccatcgggc gaccgtagtg tttctcaatt
     1981 tgtacggcct gttttgccag ttcctgcact tcttcgttgg tcagcgagaa gatgtcacgc
     2041 tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt gctcctgggt cggcgcgtaa
     2101 accatgcgga tttttttcga ccccatggtg cgacgcacga tagccgggcg attcgccgcc
     2161 agtgtcggtt tatggacgta aaactcatcc gggttaaccg caccctgcac gaccatttcc
     2221 ccaaggcccc atgcggaagt gataaacacc acctggtcaa agccggattc ggtatcaatg
     2281 gagaacatca cgccagaaga tgcgaggtcg gagcgcacca tccgctgaac accggcagag
     2341 agcgccacgc cacggtgatc gtaaccctgg tgcacacgat aagagatggc gcgatcgtta
     2401 aacagagaag caaatacatg cttcactgcc acgagaacgg cgtcaaaacc ctgaacgttg
     2461 aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca tatcttctgc ggtggcggag
     2521 gagcgcaccg caaaagaggc gttttcgtca tcggcggaaa gctgtgcata ggcttcgcgg
     2581 atggcgtttt ccagctcagg ctggaaggga gtgtcgataa tccactggcg gatttgcgcg
     2641 cccgctttcg caagctgagt aacatcgtca atatccgttt tatccagcag ttcataaatg
     2701 cgctggttta cgccgctttg gtccagaaac tggttaaacg cgtcggcggt tgtggcgaaa
     2761 ccattcggaa cggaaacacc cattccggaa aggttagtaa tcatttcacc cagggaggca
     2821 tttttgcccc caaccctgtc tacatcattc atgccgagtt ggttatacca aagcaccagc
     2881 ggtgacgagc cattgttgga catcgaacaa tccttttgtg ataaatgaac ggtttgagaa
     2941 acacatttct gcgcatttat ctttgcatat ttaaccggat gaaaaaaacg gtgaatcgtt
     3001 caagcaaata tattttttta ctttttaaga ctgatcccag cgttgcgcaa atctgctctc
     3061 ttcgacaatt tccacataaa ccatcggtat aaacgaaacc ataaaaatga aatgctgttt
     3121 tcataaaaaa taaaattgaa ggttcatttt ataaaccaga cataacgtcc tacgcttctg
     3181 tgcgttttta atttatgctt tcatagaatt atgtctgcat cacgggaaga acaaaatgga
     3241 taatgctgtt gatcgccacg ttttttatat ttctgatggt acggcaataa ctgcggaggt
     3301 attaggacac gcagtaatgt cacaatttcc cgtcactatc agcagcatta cgctgccgtt
     3361 tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag attgacgcaa tttatcacca
     3421 gacaggtgtg cgcccgctgg tcttctactc catcgtgttg ccggagattc gcgccatcat
     3481 cttgcaaagt gaaggctttt gccaggatat cgttcaggcg ctggttgccc cgctacaaca
     3541 agagatgaaa ctggatccaa cgccgattgc tcatcgtacc catggcctta accctaataa
     3601 tctcaataaa tatgatgcgc gcattgcggc gattgattac accctcgccc acgatgacgg
     3661 catttcgttg cgcaatctgg atcaggctca ggtgatcctg ctcggtgttt ctcgctgtgg
     3721 taaaaccccc accagtcttt atctggcaat gcaatttggt atccgcgcgg caaactaccc
     3781 ctttattgcc gacgatatgg ataatctggt gctacccgcg tcgctcaaac cgcttcagca
     3841 taaattgttc ggcctgacta tcgacccgga acgtctggcg gcgattcgcg aggaacgtcg
     3901 ggagaacagt cgctatgcct cgcttcgtca gtgcaggatg gaagtcgcgg aagtggaagc
     3961 gttgtaccgt aaaaatcaga tcccgtggat taacagtacc aattattcgg tagaagagat
     4021 tgccaccaag atcctcgata tcatgggcct tagtcgccga atgtactaga gaactagtgc
     4081 attagcttat ttttttgtta tgatgctaac cacccggcga ggtgtgacac acctcgcact
     4141 tgaaatcagc agcgattggt ttatcgtgat gcgcatcact tcccggcagt cctgccgtag
     4201 aagcaacaaa tttctgagac ttgtaatgaa cagaactgac gaactccgta ctgcgcgtat
     4261 tgagagcctg gtaacgcccg ccgaactcgc gctacggtat cccgtaacgc ctggcgccgc
     4321 cacccatgtc accgactccc gccgcagaat tgaaaaaata ctcaatggtg aagataagcg
     4381 actgttggtc attattggcc cctgctcgat ccacgatctc accgctgcaa tggagtacgc
     4441 cccccgtctg cagtcgctgc gcaaccagta ccagtcacgg ctggaaatcg taatgcgcac
     4501 ctattttgaa aaaccacgaa ctgttgtcgg ctggaaagga ctaatctccg atccagattt
     4561 aaacggcagc tatcgggtaa atcacggtct ggagctggcg cgcaaattac ttttacaggt
     4621 aaatgagctg ggcgtcccaa ccgcgactga gttcctcgat atggtgaccg gtcagtttat
     4681 tgctgattta atcagttggg gcgcgattgg cgcacgtact accgaaagtc agatccaccg
     4741 tgaaatggct tcggcactct cctgtccggt aggttttaaa aatggtaccg atggcaatac
     4801 gcggattgct gtggatgcta tccgcgcagc ccgcgccagc catatgttcc tctcgccaga
     4861 caaaaatggt cagatgacca tctatcagac cagcggcaac ccgtatggcc acattattat
     4921 gcgtggcggc aaaaaaccga attatcatgc cgatgatatc gccgcagcct gcgatacgct
     4981 gcacgagttt gatttacctg aacatctggt ggtggatttc agccacggta actgccagaa
     5041 gcagcaccgt cgccagttag aagtttgtga ggatatttgt cagcaaatcc gcaatggctc
     5101 tacggcgatt gctggaatta tggcggaaag tttcctgctc gaaggaacgc aaaaaatcgt
     5161 cggcagtcag ccgctcactt acggtcaatc cattaccgac ccgtgtctgg gctgggaaga
     5221 taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat acccgcttct gaatgcgtgc
     5281 ccattcctga cggaatgggc atttctgcgc aacttgttgt cttctcaaca aattactgct
     5341 tgctctggcc tgccataata ttgataataa gaatcattgt tatatcaatt attattaatc
     5401 tttatgcgtt atacggatag cagaaaactc acgcctgaaa cggacgccaa tcacaaggcc
     5461 gcttccccgc agcccatccg gcgaattacc agccagacac tgttaggtcc ggatggcaaa
     5521 ctgattatcg atcatgacgg gcaagaatat ctgctccgta aaactcaggc tggcaagctg
     5581 ctgttaacta agtagccttt aactcgagca gctgacttcc agccgtttac cccagtcagg
     5641 tggacgactg acatagtcat catctctatc gctgaaagga tttcgcaacg ccccatgcag
     5701 gcggtgcaat tccgtcatat cacccttttc tgccgcttca atcgcccgtt gcgccaacca
     5761 gttgcgcaat accagagcgg ggttaacgct ttgcatcaac tgctgacgct cactatcgct
     5821 aacctcgtct tgttgcaaac gcccccgata acgggcaaac cagccatcaa atgccgcacg
     5881 atcaataaac tcatcacgta gcggtgacgc cgtgctgtgc tgctcggtca gactcagcat
     5941 gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc agactgaata attcattcag
     6001 tagcgcgtta tcctcttttt gctccgtgat gaagcccagt ttctgccgca tccgttgtcc
     6061 ataatgcgtc aacaaaacct gctgatagct gtccagggcc tcattcaggg catctacggc
     6121 aacaaatggt gacaatgtct gcgccagacg ctgtaaattc cacaacgcga cagcaggttg
     6181 attatcaaaa ctataacgcc cttgatgatc cgagtgatta caaataaaac cgggttcgta
     6241 atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagt cccagcagcg acatgttgtc
     6301 ggtattcatc accccatgtg caaagccgac cgtctgccat tgggcaatta acgacgcggt
     6361 acgtgcgaca acatcgctaa accagagacg gtatttgtcc tcatcatctt caagatgtga
     6421 ccagtaatga cgaatagcaa agtcagctaa ctgacgaacc ttttccgact cgcggcggta
     6481 gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt gccacacgca tcagcatcgc
     6541 gcctggctcc gccgtttccc gatacactgg cgaatcgctg gtgacgatac ttaacgcgcg
     6601 ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg agactttctc gtatcgtcga
     6661 acgtaaaacc gcccgtccat cacccattcg cgaataaggc gtcaggccag cacctttcag
     6721 atgccagtcc attgtagtgc catcagcaag cagttgttcg ccgagtaaaa tgccgcgccc
     6781 atcacccagt tggcccgccc agacgccgaa ctgatgacca ctgtaaacct gcgccagtgg
     6841 tgacatacca ggcagtaggg cttcgccgcc ccagacacct gcgccatttt taaacagcga
     6901 cgatggaata ctcagcgtgt tagccagttc ggtattatgc caaatcagcc gggcattatt
     6961 taaaggcgta ggggaaagtg ctgtataggt ttctggcaat tcatcgcgcc agcgggtaac
     7021 aaaagacagg gtcatagatc ctcctgtttg atagtgtaga cggttactct cgtcttaaac
     7081 accagcaaac agaagggtta tcgctgaacc aacgtcgtta tctgatttat gggaacggca
     7141 ggccacaggc atccctggag agcatggaaa tcgaaggggg taatctgtgc caatatttct
     7201 gccgtatcaa tgcctcccgc aatgatgcaa ttgcaacaag gggaaatttg cgcctggatc
     7261 gcacgaataa acggttcgaa ggagcggtgc gtgatttgtt gctgaataaa acttttatcc
     7321 agcatgacgc gggtgaaaag accatcaaaa accgctttca ttgtgctatt gcctgctccc
     7381 aaattaccta acacaagggg atacacttgc gataaggata aaagaccgcg attatctttt
     7441 ccttcattga gatgcgggta attctcatta atcaacagtt caataaacgg atattttaat
     7501 agttcgcctg cgtaattatc cctctctaat aataatgtag ccacttgtgg cgttaaatta
     7561 agccaggcaa agagtttatg ttgaataaaa aaatgttggc atgattttag taattccaac
     7621 tgctctgaaa ataattgcca atgttgctcc tcggtaagtt gcgctataac ccgactcgtc
     7681 gggatacgca ccgtgccatc ttcactggag aaatgggtaa ttaactcaac gccgacaagg
     7741 acttgctggt tatctcttat cggcaggaaa tagcaatcag aatgataaag attctccaaa
     7801 aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt cagtatccgg ctaaaaggga
     7861 tgaggccata agacataaca attacagaag gagtaacttt catttgttcc atgttaacca
     7921 ctttttcagg ggtccttttt ttagattatc ctgattataa acgaataatc catttacgga
     7981 atttttgtct gcaaaatact actgtattca ggagtaaatg gacggcgatg tatcatcgcc
     8041 gtccattttt cgac
//
LOCUS       DAZOZY010000181         8040 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZOZY010000181 DAZOZY010000000
VERSION     DAZOZY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.182"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..105
                     /gene="yjbL"
                     /locus_tag="ACHURQ_003414"
     CDS             <1..105
                     /gene="yjbL"
                     /locus_tag="ACHURQ_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR0825122.1"
                     /translation="YAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            96..836
                     /locus_tag="ACHURQ_003415"
     CDS             96..836
                     /locus_tag="ACHURQ_003415"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR0825123.1"
                     /translation="MFLIEQINDLKMWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFA
                     INSICNPGDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGF
                     LTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQR
                     DMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSF
                     DPTSKEEIKILTDTSKLIYDLFGVKSEK"
     gene            1199..2236
                     /gene="dusA"
                     /locus_tag="ACHURQ_003416"
     CDS             1199..2236
                     /gene="dusA"
                     /locus_tag="ACHURQ_003416"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR0825124.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2370..2612
                     /gene="pspG"
                     /locus_tag="ACHURQ_003417"
     CDS             2370..2612
                     /gene="pspG"
                     /locus_tag="ACHURQ_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR0825125.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2778..3761)
                     /locus_tag="ACHURQ_003418"
     CDS             complement(2778..3761)
                     /locus_tag="ACHURQ_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR0825126.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3844..5259
                     /gene="dnaB"
                     /locus_tag="ACHURQ_003419"
     CDS             3844..5259
                     /gene="dnaB"
                     /locus_tag="ACHURQ_003419"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0825127.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5312..6391
                     /gene="alr"
                     /locus_tag="ACHURQ_003420"
     CDS             5312..6391
                     /gene="alr"
                     /locus_tag="ACHURQ_003420"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR0825128.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6644..7837
                     /gene="tyrB"
                     /locus_tag="ACHURQ_003421"
     CDS             6644..7837
                     /gene="tyrB"
                     /locus_tag="ACHURQ_003421"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR0825129.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1968 a         1898 c         2078 g         2096 t
ORIGIN      
        1 tatgcagcgc tgcgtcatcg ggatataatt attgcaaaaa tagatttgtt tattcaacga
       61 atcacgcata atttatggca tgcacgtaaa aaaatgtgtt tttaattgag caaataaatg
      121 atctcaaaat gtgggtcaat aagtatattg acgattgcac tgatgaggat ttaaacgatc
      181 gtgactttat tgcatcagtt gttgaccggg ctatttttca tttcgcgatt aatagtatat
      241 gtaatcctgg ggataataaa gatgcgactc ccattgaaca atgtactttt gatgtagaaa
      301 ctaagaatgg ccttccctcc acggttcagc tattttatga ggaatctaag gataatgaac
      361 ctttagcgaa tatacatttt caagcaatag gttctggttt tttaacgttt gttaatgcct
      421 gccaggaaca tgatgacaac agcttaaaat tatttgcttc gctgttaatt tcactttcat
      481 attctagtgc ctacgcagat ttatcagaaa cagtgtatat taatgaaaat aatgagagct
      541 acctgaaagc tcagtttgaa aaattatctc aacgtgatat gaagaagtac ctgggagaga
      601 tgaagcgtct ggctgatggg ggagaaatga attttgatgg ctatctggat aagatgtcac
      661 atctggtgaa tgaaggaacg ctcgatcctg atattttaag caaaatgcga gatgctgcac
      721 cacaattaat tagcttcgcg aagtcgtttg acccaacctc aaaggaagag attaaaatac
      781 ttacagacac ttctaaatta atttatgatt tgttcggggt taaatcggag aaataatatg
      841 tgaagttctt cgatagtatg gaaggcatta tataaaagga cccaatattt attgggttct
      901 tttttctcta tcaatgctat tagcagggag atatatcacc agagtttaat gtgtgatttt
      961 ttatttatcg tcgaacctgg attgtttatc attggcctta acaaagttaa cggctaataa
     1021 gattatttcc atcacttcgt gagagcttca tgccttgaga ggatctcaat tttcttttgc
     1081 aatgagacag gcgcttcctg ttgttatggt atagtacccc gctattgagc ctcctgaata
     1141 gtgatgctga ataacataac cccatgatat atcgataaaa taatctctac atttgaaaat
     1201 gcacggtaat tctgaaatgc aaaaaatcaa ccaaaccagc gcaatgcctg aaaaaactga
     1261 cgttcactgg agtggtcggt ttagcgttgc accaatgctc gactggacgg acagacattg
     1321 ccgctatttc ttgcgtctgc tttcccgcaa tacgttgctg tataccgaaa tggtgaccac
     1381 aggggcgatt attcacggta aaggtgatta cctggcgtac agtgaagaag aacatccggt
     1441 agcgttgcaa ctcggcggta gcgatccggc ggcgctggca cagtgtgcga agctggcaga
     1501 agcgcgtgga tatgatgaga tcaacctgaa tgtcggctgc ccgtctgacc gggtgcagaa
     1561 cggcatgttt ggtgcgtgtc tgatgggtaa tgcgcagctg gttgccgact gcgtgaaagc
     1621 gatgcgcgat gtggtgtcga ttccagtgac ggtgaaaacg cgtattggca tcgatgacca
     1681 ggacagctat gaatttctct gcgatttcat caacaccgtt tccggcaaag gcgagtgtga
     1741 gatgtttatc atccacgcac gtaaagcctg gctttcgggg ttaagcccga aagaaaaccg
     1801 tgaaatcccg ccgctcgatt atccgcgtgt gtatcaactg aagcgtgact ttccgcatct
     1861 gacaatgtcg attaacggtg gtatcaagtc gctggaagag gccaaagcac acctgcaaca
     1921 tatggatggc gtgatggtcg ggcgcgaggc gtatcagaat ccgggtattc tggcggcggt
     1981 agaccgggag atctttggtt cctcggatac cgatgccgat ccggtggcgg tagtgcgcgc
     2041 catgtatccg tacattgagc gtgaactcag ccaggggacg tatctcggcc atattacccg
     2101 gcatatgttg ggcttgttcc agggtattcc tggcgcgcgg cagtggcggc gttatttaag
     2161 tgaaaatgcc cataaagcgg gtgcagacat taatgtgctg gaacacgcgc tcaaactggt
     2221 ggcggataag cgttaacttt tcaccaaaaa gtagtcaaat tcaccacgcc ctgcgcaccg
     2281 tcgcggggcg ttttgctgtt aaatcaatag attatttttg gcatgattct tgtaatgcca
     2341 gcaagagatt tcatatttgg gagagcatca tgctggaact actttttgtg attggctttt
     2401 ttgtcatgct gatggtcacc ggcgtttcgt tgctgggcat tatcgccgcg ctggttgtgg
     2461 cgacggccat tatgttcctc ggcggtatgc tggcattgat gattaagttg ctgccgtggt
     2521 tactactggc gattgcggtg gtgtgggtta ttaaggcgat taaagcacca aaagtgccga
     2581 aatatcagcg ttatgaccgc tggcgttact aagggattgt gcggatgatc acaacctaag
     2641 gttttatcct tagaacaaaa taggaattga taatcaaatc tgtcactatt gcgcctctaa
     2701 cagattcatc gtgctgtacc ctacatacag ccgaactata aaaagaaagg gcttcccagg
     2761 tggaagccct atttctttta tggaatcaac aggctggaac cttgcgtcgc ccggctttcc
     2821 agaatctcat gcgcacgctg cgcatccttc agcggatatt tctgctgctc ggcgacatcg
     2881 accttaatca caccgctggc aatcaaagag aacagttcat tactggcctc ggttaattcc
     2941 tcccgcgtgg tgatatagcc ttgcagggaa gggcgtgtca catacaacga gcctttttga
     3001 ttgagaatgc ctaagttcac accggtaacc gcacctgatg agttgccaaa actgaccatt
     3061 aagccgcggc gttgcaggca atccagcgac cgttcccagg tgtctctgcc cacggaatcg
     3121 tacaccacgc gcactttctt accgccggtg atctctttta accgctcgac cagatcctct
     3181 tcacgatagt taataacctg ccacgcgccc gcttttagcg cgctctgcgc tttttgcgcg
     3241 gttcctacgg tgccgataag tttcgcgccc agggcttttg cccactggca ggcaattaag
     3301 ccaacgccgc cagccgctgc gtggaacagg aactgttcat cgggtttaat ttcataggtt
     3361 ttgcgcagca gataataaac cgttaagcct ttcaggaagg atgccgcagc ttgctcaaaa
     3421 gaaattgccg caggcagaat cgccgcttta tccgcattaa tgttatgcac agagctgtaa
     3481 gcgcctaacg ccgactgcgc atagactaca cgatcgcctg ccttaatatg ctttacacca
     3541 ctgccgactt tactcacgat gcctgccgct tcggtaccta atccgctggg tagtgatggc
     3601 ggcgggtaaa ggccgctgcg gatatacgtg tcgataaaat tgatgccgat ggctttattt
     3661 tcgacctgga tttcattctc tgccggatcg gcaggagtga actctacggc ttgaagtact
     3721 tccgggccac cgtgcttgtg aaattcaatt cgtgttgcca tgtgtcctcc agaacgtatc
     3781 gtcagggtct gcttcatatg gtaaagtttc gacccattct ttatctcggt aactccattc
     3841 actatggcag gaaataaacc cttcaacaaa cagcaggctg aaccccgcga acgcgatcca
     3901 caagttgccg ggctgaaagt gcctccgcac tcgatcgaag cggagcagtc ggtgttgggc
     3961 ggtttaatgc tggataacga acgctgggac gatgtagccg agcgtgtggt ggcagacgat
     4021 ttttacaccc gcccacaccg ccatatcttt actgaaatgg cgcgtttgca ggaaagcggt
     4081 agtcctatcg atctgattac ccttgcggaa tcgctggaac gccaggggca actcgatagc
     4141 gtcggtggtt ttgcttatct ggcagagctg tcaaaaaata cgccaagtgc ggcgaacatc
     4201 agtgcttatg ctgacatcgt gcgtgaacgt gccgttgtcc gtgagatgat ctcggttgcg
     4261 aatgagattg ctgaagccgg ttttgatccg caggggcgta ccagcgaaga tctgctggac
     4321 cttgctgaat cccgcgtctt taaaattgcc gaaagtcgtg caaacaaaga cgaagggccg
     4381 aagaacatcg ccgatgtgct cgacgcaacc gtggcgcgta ttgagcagtt gtttcagcag
     4441 ccacacgatg gcgttaccgg agtaaacacc ggttatgacg atctcaacaa aaaaaccgct
     4501 ggcttgcagc cgtcggattt gatcatcgtc gccgcgcgtc cgtcgatggg taaaacaaca
     4561 tttgcgatga acctcgtcga aaacgcggcg atgttgcagg ataaaccagt acttatcttc
     4621 tcgctggaga tgccttcaga acagattatg atgcgttctc tggcgtcgct gtcgcgcgtt
     4681 gaccagacta aaatccgtac cgggcagctc gatgatgaag actgggcgcg catttccggc
     4741 accatgggta ttttgctcga aaaacgcaat atctatatcg atgactcctc cggcctgacg
     4801 ccaacggaag tgcgttcccg cgcacgccgt attgcccgtg aacacggcgg catcgggctt
     4861 atcatgatcg actacctgca actgatgcgc gtaccggcgc tttccgataa ccgtacgctg
     4921 gaaattgcag aaatctctcg ctcgctgaaa gcactggcga aagaactgaa cgtgccggtg
     4981 gtggcgctgt cccagttgaa ccgttctctg gaacaacgtg ccgacaaacg cccggtcaac
     5041 tccgacctgc gtgaatctgg ctctatcgag caggatgcgg acttgattat gtttatctat
     5101 cgtgatgagg tgtatcacga aaacagtgat ttaaaaggca tcgcggaaat tattatcggt
     5161 aaacaacgta acggcccaat cgggacggta cgcctgacct ttaacggtca atggtcgcgc
     5221 ttcgacaact atgcggggcc gcagtacgac gacgaataat aattatttta tgaattaggt
     5281 aattaaagca aacacttatc aaggaacaca aatgcaagcg gcaactgttg tgattaaccg
     5341 ccgcgctctg cgacacaacc tgcaacgtct tcgtgaactg gcccctgcca gtaaaatggt
     5401 tgcggtggtg aaagcgaacg cttatggtca cggtcttctt gagaccgcgc gaacgctccc
     5461 cgatgctgac gcctttggcg tagcccgtct cgaagaagct ctgcgactgc gtgcgggggg
     5521 aatcaccaaa cctgtactgt tactcgaagg cttttttgat gccagagatc tgccgacgat
     5581 ttctgcgcaa cattttcata ccgccgtgca taacgaagaa cagctggctg cgctggaaga
     5641 ggctagcctg gacgagccgg ttaccgtctg gatgaaactc gataccggta tgcaccgtct
     5701 gggcgtaagg ccggaacagg ctgaggcgtt ttatcatcgc ctgacacagt gcaaaaacgt
     5761 tcgtcagccg gtgaatatcg tcagccattt tgcgcgcgcg gatgaaccaa aatgtggcgc
     5821 aaccgagaaa caactcgcta tctttaatac cttttgcgaa ggcaaacctg gtcaacgttc
     5881 cattgccgcg tcgggtggca ttctgctgtg gccacaatcg cattttgact gggtgcgccc
     5941 gggcatcatt ctttatggcg tctcgccgct ggaagatcgc tccatcggtg ccgattttgg
     6001 ctgtcagcca gtgatgtcac taacctccag cctgattgcc gtgcgtgagc ataaagccgg
     6061 agagcctgtt ggttatggtg gaacctgggt aagcgaacgt gatacccgtc ttggcgtagt
     6121 cgcgatgggc tatggcgatg gttatccgcg cgccgctcca tccggtacgc cagtgctggt
     6181 gaacggtcgc gaagtaccga ttgtcggacg cgtggcgatg gatatgatct gcgtagactt
     6241 aggtccacag gcgcaggaca aagccgggga tccggtcatt ttatggggcg aaggtttgcc
     6301 cgtagaacgt atcgctgaaa tgacaaaagt gagcgcttac gaacttatta cgcgcctgac
     6361 ttcaagggtc gcgatgaaat acgtggatta atcgttctgt aatatttgat tgtctgtgcc
     6421 ggatgcggcg tgaatgcctt atccggccaa taaaatccta aaaattcaat aagttgatgt
     6481 tctttcatgc tcttataaag gtcgtgcctc tggcggatgt tcgtttgtca tgagtctcac
     6541 tctgttgcta attgccgttc gctcctgaac atccactcga tcttcgcctt cttccggttt
     6601 attgtgtttt aaccacctgc ccgtaaacct ggagaaccat cgcgtgtttc aaaaagttga
     6661 cgcctacgct ggcgacccga ttcttacgct tatggagcgt tttaaagaag accctcgcag
     6721 cgacaaagtg aatttaagta tcggtctgta ctacaacgaa gacggaatta ttccacaact
     6781 gaaagccgtg gcggaggcgg aagcgcgcct gaatgcgcag ccccatggcg cttcgcttta
     6841 tttaccgatg gaagggctta acagctatcg ccatgccatt gcgccgctgc tgtttggtgc
     6901 ggaccatccg gtactgcaac aacagcgcgt agcaaccatt caaacccttg gcggctcagg
     6961 ggcattgaaa gtgggcgcgg atttcctgaa acgctacttc ccggaatcag gcgtctgggt
     7021 cagcgatcct acctgggaaa accacgtagc aatattcgcc ggggctggat tcgaagtgag
     7081 tacttacccc tggtatgacg aagcgactaa cagcgtgcgc tttaatgacc tgttggcgac
     7141 gctgaaaaca ttacctgccc gcagtattgt gttgctgcat ccatgttgcc acaacccaac
     7201 gggtgccgat ctcactaatg atcagtggga tgcggtgatt gaaattctca aagcccgcga
     7261 gcttatccca ttcctcgata ttgcctatca aggatttagt gccggtatgg aagaggatgc
     7321 ctacgctatt cgcgccattg ccagcgctgg attacccgct ctggtgagca attcgttctc
     7381 gaaaattttc tccctttacg gcgagcgcgt cggcggactt tctgttctgt gtgaagatgc
     7441 cgaagccgct ggccgcgtac tggggcaatt gaaagcaaca gttcgccgca actactccag
     7501 cccgccgaat tttggtgcgc aggtggtggc tgcagtgctg aatgacgagg cattgaaagc
     7561 cagctggctg gcggaagtag aagagatgcg tactcgcatt ctggcaatgc gtcaggaatt
     7621 ggtgaaggta ttaagcacag agatgccaga acgcaatttc gattatctgc ttaatcagcg
     7681 cggcatgttc agttataccg gtttaagtgc cgctcaggtt gaccgactac gtgaagaatt
     7741 tggtgtctat ctcatcgcca gcggtcgcat gtgtgtcgcc gggttaaata cggcaaatgt
     7801 acaacgtgtg gcaaaggcgt ttgctgcggt gatgtaatgc aggaaagcag gctggagtta
     7861 cccagcctgc agtgaaatta aactgtcgtc gctttcacta tttctttgta gatgattttt
     7921 ttgatgccgt tgttctacgt gagagataat aaaacgttgt cagttctttt attgttaagt
     7981 ttatcccaat tatctggaat tcctttccct attttttcgt gtggagtaat aatttctgca
//
LOCUS       DAZOZY010000182         8008 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZOZY010000182 DAZOZY010000000
VERSION     DAZOZY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8008
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.183"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHURQ_003422"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHURQ_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHURQ_003423"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHURQ_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR0825130.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1371..2300)
                     /gene="fdhE"
                     /locus_tag="ACHURQ_003424"
     CDS             complement(1371..2300)
                     /gene="fdhE"
                     /locus_tag="ACHURQ_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR0825131.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2297..2932)
                     /gene="fdoI"
                     /locus_tag="ACHURQ_003425"
     CDS             complement(2297..2932)
                     /gene="fdoI"
                     /locus_tag="ACHURQ_003425"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR0825132.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2929..3831)
                     /gene="fdoH"
                     /locus_tag="ACHURQ_003426"
     CDS             complement(2929..3831)
                     /gene="fdoH"
                     /locus_tag="ACHURQ_003426"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR0825133.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3844..6894)
                     /gene="fdnG"
                     /locus_tag="ACHURQ_003427"
     CDS             complement(3844..6894)
                     /gene="fdnG"
                     /locus_tag="ACHURQ_003427"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6307..6309),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR0825134.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7088..7921
                     /gene="fdhD"
                     /locus_tag="ACHURQ_003428"
     CDS             7088..7921
                     /gene="fdhD"
                     /locus_tag="ACHURQ_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR0825135.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1859 a         2061 c         2078 g         2010 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaaatac gagagatggt gtcgtcacac
     1321 cctatgagct gtaatccggg cgacctgcgc cacccggaaa acgtcagaga ttactctcct
     1381 tcacccggaa acaggaacgg gttaatggaa ctacgggcgt agccttcttg ctccattcgc
     1441 gcgtccagta ccagagaggc gaggtcatct gccacggctt caactttcgg ttctttttcc
     1501 tgataaagaa tcttcaggta agtgccgcag tcatcgcagc tttcggcttt aatcgcggcc
     1561 tgttcgtcat ccagcgacca gtaatgcagt ttgccgctct gctcgcagtt gctgcatttt
     1621 acgcgcacta cgtgccattc ggtttcacac aggttgcagt gcaggtaacg cagaccctga
     1681 gtggtgccaa tttgcaccat gctggacacc ggcatagagc cacataccgg gcaatattga
     1741 cgttgttcgc cgtattcagc gcgggctttg ccggggatca gattggccat ctgcgcccag
     1801 tagagcgaca gcgcagccca gataaacggc gctttatcgc tgctgacgga cgagaaatca
     1861 gaggcaaaca gcgcgctggc catatcttcc agctcctgag tcgatgcctt ctccagattc
     1921 tcaatcactg ccagcgccgg gccgctcatt tcaggtttca gctcagcaat tagcgccatc
     1981 agcagctttt gccagtgctt atcacgcggc agaacgtgaa tatccagcgg gggcttgccc
     2041 tgtgcgctgg cttctttaat gcgcgcggtc agatccatct ccagcggatg gtcgtacagc
     2101 accacttcct gggcgtgggc gataagcgca gcaaagcgca ggtaatcacc cagcggatta
     2161 ttttctgcca gctcgcgcag acgctcggcg cggcggttgt ataaattctt gagccgaggg
     2221 aacaataacg gcggaatcat atccgccgta cgtttctcgc tcgaacccag ctcatcttgc
     2281 gggattatgc gaatactcat tcagctttct tttccgttgt cttgcggacc tcacggtacc
     2341 agcgcgggtg atgtttcttc gcccaggcgc tggtaaccca tccttccacc atggcggtaa
     2401 tcgtgccttt cacccaaagg gcggcgtaga tatgcaccat gataaccaca attaacgcca
     2461 ctgcggcaaa tgaatgcagc attaacgcga atcggatcac cgggattgag aaagcaggcg
     2521 caaaataagg acgccagata atcacgccgc tcaccagcag cagaaccagg aaaataatcg
     2581 ccgcccagaa aacgcatttc tgaccgaagt tataacgccc ggtgtcacct acttcctcgt
     2641 tgacgacgat cttacgaata ttcttcgccc aaaagatatc atcccgattg attaggttgt
     2701 gatgccagta acggaaaaac atgatgatga acgaggcaaa cataaccacg ccgacaaacg
     2761 ggtgcagaat tcgcgccagc tgcggtgtgc ccatgatttg catcaaccag ttgaaggacg
     2821 ggaacaaaaa gcccagcccg ctcaccgccg ccaggatgaa gcagaaggcg gtgatccagt
     2881 ggttgatacg ttccggcgcg gtgtagcgca cgatggtgtc acgtcgtttc atttgcgctc
     2941 ctcgtctttc tcttcgtgca gattattctc ttcctcatcc gcacggttcg gaccgacacc
     3001 cacgtagtgg aagatactgg ccgcaaaggt agccgcaaag ccaacagccg cgagcggttt
     3061 ccagatgcct ttccagaatt tcacggtttc gctgatttcc gggttctccg gcaagccatg
     3121 atacagattt ggcttgtcag catggtgcag cacgtacatg acgtgtgtac caccgacgcc
     3181 tgccggatcg tacagacccg cattgtcgta accgcgggtt ttcagctcag caacgcgctc
     3241 gctcgccagc gttttcatcg actctttcgt accgaagtga atcgcgcccg ttgggcaggt
     3301 cttcacgcag gctggttctt gcccaaccac cacgcggtca acgcacagcg tacatttgta
     3361 gacgcggttg tcttccgggt tgaggcgcgg aatgtcgaac ggacagcccg caatgcaata
     3421 accgcagcca atgcactgct cggactggaa gtcgacgata ccgttggcat actgaataat
     3481 tgcaccttcc gccgggcacg ctttcaggca gcctggatcg gaacagtgca tacagccgtc
     3541 tttgcggatc agccattcca gtttgtcgtt ctgctccact tccgagaagc gcatcaccgt
     3601 ccacgatttg gcgcttaaat cattggggtt gtcgtacacc ccaatgttat tgccgacggt
     3661 atcgcgaatg tcgttccact ctgaacacgc cacctgacag gctttacagc cgatacaggt
     3721 ggtgacgtcg atgagtttcg ccacttcttc ctggaagtcc cgcgcctgag gcgcgggggt
     3781 cagaccgtta gtcgcggaac gacgaatgat atcttgcgat tgataagcca tatgtcgtct
     3841 ccgttacacc ttttccacat tcacaaggaa ggacttaaac tccggcgtct gcgtgttcgc
     3901 atcaccaacg aatggcgtta acgtattggc aataaagcct tttttagcta cgccttcata
     3961 gccccagtga atcggaatac cgatggtatc gatatctttg ccgttcgctt tcagcgtgcg
     4021 aatacgtttg gtcaccaccg ctttggcttt gatatagcca cggttggagg agactttcac
     4081 ggtatcgccc tgggcaatgc caagtttatt cgccagcgac tccccgattt ccacaaactg
     4141 ctctggttgc aaaatcgcgt tcaacagcgc gtgtttggtc cagtagtgga agtgctcggt
     4201 cagacgatag gtggttccga catacgggaa cttatcggct ttacccaatg cttcggcgtc
     4261 gtctttaaag attcgcgcag ccgggttcga gataacgttt ggatgcagcg ggttagttcc
     4321 cagcggcgtt tcaaacggct cgtagtgttc cgggaacgga ccttccgcca tcttatcgag
     4381 ggcaaacaga cgccccatgc cttcctgctg catgataaac ggcccgacgc cgctgcccgg
     4441 aggcgctgcg ctgtagtccg gaatatccca gccggtccac ttagtgccgt cccatttcag
     4501 caactgacgc ttcggatccc acgggttacc ctgcggatct gcggaggcgc ggttatacag
     4561 aatgcggcgg ttaagcggcc atgcccatgc ccagcccagc gtgttaccga ggccagacgg
     4621 atcggcgtta tcacgacgcg ccatctggtt gccttccggc gtccagctac cggcgaaaat
     4681 ccagcagcca caggaggttg taccgtcatc gcgcaattgg gcgaacgagc taagttgttg
     4741 gcctttcttg acgataaccg caccggttgc cggatcggta atatcggcca gcgccttacc
     4801 gttgctctcc atcgccactt cttccgactt cggctcatgc ggaatggcgt agttccaggt
     4861 catgttcagc acctgatccg ggtttgcgcc accctgttcg gcatacatct tgcgcaagcg
     4921 caggaagata ccggagagga tctcgccatc agtcagcgca atccctgggg cgtccgcacc
     4981 tttccagtgc cactgcaacc agcgacctga gttaacgatt gaaccgttct cttctgcgaa
     5041 gcaggtcgat ggcagacgga acacttcggt ctggatcttc gacgagtcaa cttcgttcag
     5101 ctcaccgtgg ttctgccaga agttagaggt ttcagtgttc agcgggtcga tggtgacgag
     5161 gaacttcagt ttcgacagac aaccgatcac tttgtttttg ttcgggaatg aggcaacagg
     5221 gttaaagccc tggcagatat agccattgac cttgccctct ttcatcatct cgaagtattg
     5281 caggacgtcg tagcctttat cccacttcgg caaccagtca aagccccagc tattttccgc
     5341 cgtcgcttta tcaccaaaga aggccttcat catagagacg aagaatttcg ggtagttgcc
     5401 ccagtagttt acctggcctt ccagcagcgg ttttggcgtg ttggcggtaa ggtaggtttg
     5461 cagatcggtc tgcttctcgc ttggcagcgt catgtaacct ggcaggctct gcgacagcag
     5521 ccccaggtcc gtcagcccct gaatattgga gtgaccgcgc agggcgttaa cgccgccgcc
     5581 tgccatcccc atattgccga gcagcagctg gatcatcgcc atcgtacgaa tgttttgcgc
     5641 accaacggag tgttgcgtcc agccgagggc atacaggaac gaggcagttt tatcgtgagc
     5701 actggtttct gcgatgtatt cgcagacttt caggaacgcg tcttttggcg taccacagat
     5761 gttttcaacc acatctggcg tgtagcggga aacgtgctgt ttcagcaagt tccacacgca
     5821 gcgcggatgt tgcagcgtgg tatcgcgttt ggcgaagccg ttttcgtcca gttcgtaagt
     5881 ccaggaggat ttatcgtact tgcgtttttc cgcgtcatag ccggtgaaca ggccatcttc
     5941 aaagccgtaa tcctcacgca cgatcaggct ggcgttggta taggcttcgg tgtattcgcg
     6001 gttgaatttt tcattgttca gcaggtacag caatacgcct gacaggaaag caatgtcagt
     6061 accggaacga ataggggcat agtagtcagc caccgccgcc gtacgcgtaa agcgaggatc
     6121 gatcacaatc agcttcgcgc cattgtgaat tttggcttcc atcgcccagc ggaacccgac
     6181 cgggtgagct tcagcggcgt taccgcccat caccacgacg aggttggcgt tcttgatgtc
     6241 gacccagtgg ttggtcatcg caccgcgacc aaatgttgga gcaagacttg ctaccgttgg
     6301 tccgtgtcag acacgcgcct ggttgtcgac cgcgagcata cccagcgcgc gggagaattt
     6361 ttgcgttaaa tagccggttt cgttactcga cgcggaagca cacagcatcc cggtggagag
     6421 ccagcggtta acagtcacgc cttcggcgtt ttgcgcaatg tagttagcat cgcggtcttc
     6481 tttcatcagt ttggcgatgc gatcaaacgc ctcttcccaa ctgatttgct gccatttatc
     6541 agaacctggc gcacggtatt ccggaaactt cagacggctt tcggagtgga tgaaatccac
     6601 caggccagcg cctttcggac aaagtgcacc gcggttgacc gggtgatccg gatcgccttc
     6661 gatatggaag atagatgctt tggcgttttt tgctccgtca ccgaggctgt acatcaacag
     6721 cccacagcct acggaacaat aggtgcaggt attacgggtt tcgcgggtgc gcagcagttt
     6781 atactgccgg gtttccgcga gtgctacgct gggtgcaaaa cccagtgccg ctgccgtggt
     6841 gcctgccata ccgccagcgc agatcttaaa gaactgcctt ctgctgacct gcatggattg
     6901 ctccttgttt cgacattgtc acgtcccatt tacattcgct tgctgcgtgt gcagggagtg
     6961 ggagttattt ttctttgcgg aaggggccgc aaaggtccag aattggctca atttccctcc
     7021 atccaggaag ggtttgtaac agaataccat aatgttggtg tgtgtgttct tatctggtta
     7081 agagaaagtg aaaaaaacac agcgaaaaga aatcgaaaat gtgacaaata tcacaggtgt
     7141 tcgtcaaatt gagttatggc ggcgtgatga tttacaacac ccacggttag atgaggtcgc
     7201 ggaagaagtt cccgttgcgc tggtctacaa cggcatttcg catgtggtga tgatggcgtc
     7261 gcccaaagac cttgagtact ttgcgctcgg tttttcgctt tccgagggga taatagaaag
     7321 tccgcgcgat atcttcggca tggatgtcgt tccttcctgt aatggtcttg aagtacaaat
     7381 tgagctttcc agccgccgct ttatggggtt gaaggagcgc cgccgggcgc tggcgggacg
     7441 tacgggctgt ggcgtatgcg gcgtggagca acttaatgac atcggaaaac cggtgcagcc
     7501 gctaccgttc acccagacgt ttgatctcaa caaactggat gatgcattac gtcatctcaa
     7561 cgattttcag ccagtggggc aactgactgg ttgtactcac gccgctgcct ggatgttgcc
     7621 atctggcgaa ctggtcggcg ggcatgaaga cgtgggtcgc catgtggcgc tggacaaact
     7681 gttaggccgc cggtcacaag aaggggaaag ctggcagcaa ggtgcggtac tggtttccag
     7741 tcgtgccagt tatgaaatgg tgcaaaagtc ggcgatgtgc ggcgtagaga ttttgtttgc
     7801 ggtgtctgcc gcgaccacgc ttgctgtaga agtggctgag cgctgtaatc tgacactggt
     7861 aggtttttgt aaaccgggtc gggcaacggt ttatacccat ccgcagcgtt tgagcaatta
     7921 attaaaataa gtcctgcgat atatattaaa ttgcaggact tattcatttc gtgaatttta
     7981 ttattttatt tataaataaa acgcgtgt
//
LOCUS       DAZOZY010000183         7994 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZOZY010000183 DAZOZY010000000
VERSION     DAZOZY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.184"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(344..1375)
                     /gene="rsmC"
                     /locus_tag="ACHURQ_003429"
     CDS             complement(344..1375)
                     /gene="rsmC"
                     /locus_tag="ACHURQ_003429"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR0825136.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1478..1891
                     /gene="holD"
                     /locus_tag="ACHURQ_003430"
     CDS             1478..1891
                     /gene="holD"
                     /locus_tag="ACHURQ_003430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR0825137.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1860..2306
                     /gene="rimI"
                     /locus_tag="ACHURQ_003431"
     CDS             1860..2306
                     /gene="rimI"
                     /locus_tag="ACHURQ_003431"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR0825138.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2321..2998
                     /gene="yjjG"
                     /locus_tag="ACHURQ_003432"
     CDS             2321..2998
                     /gene="yjjG"
                     /locus_tag="ACHURQ_003432"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR0825139.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3089..4678
                     /gene="prfC"
                     /locus_tag="ACHURQ_003433"
     CDS             3089..4678
                     /gene="prfC"
                     /locus_tag="ACHURQ_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR0825140.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5071..5676
                     /gene="osmY"
                     /locus_tag="ACHURQ_003434"
     CDS             5071..5676
                     /gene="osmY"
                     /locus_tag="ACHURQ_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR0825141.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5803..5964
                     /locus_tag="ACHURQ_003435"
     CDS             5803..5964
                     /locus_tag="ACHURQ_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR0825142.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6086..7159
                     /locus_tag="ACHURQ_003436"
     CDS             6086..7159
                     /locus_tag="ACHURQ_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR0825143.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7156..7938
                     /locus_tag="ACHURQ_003437"
     CDS             7156..7938
                     /locus_tag="ACHURQ_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR0825144.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1909 a         2068 c         2167 g         1850 t
ORIGIN      
        1 gggggacttg aacccccacg tccgtaagga cactaacacc tgaagctagc gcgtctacca
       61 attccgccac cttcgcatac catcaattct taaaaagaat tgctaccacg gaggcgcatt
      121 ctagtggttt tcagcttttc gtcaatagtt aattatcgcc ggaggtgtaa ttgctggaaa
      181 aatgtccatc aggaaactag cgtgcaggtt tggtaggcat gcgggggcag atgccagatg
      241 cgacgctggc gcgtcttatc tggcctacga agggctaacg tgcaggtttt gtaggtcgga
      301 taaggcgttc acgccgcatc cgacacggta ttcggcgaga taattaacct ttcttcgcct
      361 ggcgggtcat aatggcgcga tacaccttga aacgcccggt ttgcgcaatc acttcatgga
      421 agccaaatgt ctcatccagc acgtccgggt aaggcaggaa ggcgttcgct acaattcgca
      481 gctcgccgcc gctattaaga tgacgcaccg cgccgcgaat cagcgtttgc gccgcatcca
      541 ggctggtttg catcccatcg tggaacggcg ggttggagat gatcatatca aaacgacctt
      601 tcacctcgga aaagacgttg ctggcaaaga cttcaccttc aacacagttg gccgcaagtg
      661 ttgcgcggct ggcttctacc gctggcgcag agacatcgca caaggtgaga cgaattttcg
      721 gcgaatggcg cgcaaaggca actgaaagca cccccgcgcc acagccgaca tccagcactt
      781 tacctttcgt gtgcggagtt aacgtcgaga gcagcaactg gctaccgaca tccagaccgt
      841 cgcggctaaa cacgccaggc agcgttttga ccgtcaggcc atcgacgctg tattcgcccc
      901 agaatttatc ggcatcaaat accggctgtt tttccagacg accaaaatag aggccacagc
      961 gacgagcgct gtcgacttta ttcaacggcg cataatctgc cagcatctgc tcggcgctgc
     1021 gcacgccgct gcggttctcg ccaacgacaa aaatatctgt ccccactggc agcagagaaa
     1081 gtaaattcat caactggaac tgggcttccg gtttgttctt cggccagtag taaatcagcg
     1141 tatcgcaatc tgcgacgtca tccaccgtgg cgaccagact gaaacgggcg ttatccccca
     1201 tctggcggct taatacctgc cagtggtgga attgctgggt atgagcacgg ctggccgcgg
     1261 tatctaaacg cgcgggcagg tcatcctgta agtctccggc aaacagaata cggctttgtt
     1321 cgaaatcatc actgtgacgc agcaagactt cacttgccgg ggtaaatgca gacatggaat
     1381 gctcctcaat tgatactggc ggcgattata gccatatgtt ggcgcggtat cgacgaattt
     1441 gctatatttg cgcccctgac aacaggagcg attcgctatg acatcccgac gagactggca
     1501 gttacagcaa ctgggcatta cccagtggtc gctgcgtcgc cctggcgcgt tgcaggggga
     1561 gattgccatt gcgatcccgg cacacgtccg tctggtgatg gtggcaaacg atcttcccgc
     1621 cctgaccgat cctttagtga gcgatgttct gcgcgcatta accgtcagcc ccgatcaagt
     1681 gctgcaactg acgccagaaa aaatcgcgat gctgccgcaa ggcagtcgct gcaacagctg
     1741 gcggttgggt actgacgaac cgctatcact ggaaggcgct caggtggcat caccggcgct
     1801 caccgaatta cgggcaaacc caacggcacg cgccgcgtta tggcaacaga tttgcacata
     1861 tgaacacgat ttcttccctc gaaacgactg atttaccagc ggcttaccac attgaacaac
     1921 gcgcccacgc ctttccgtgg agtgaaaaaa cctttgccag caaccagggc gagcgttatc
     1981 tcaactttca gttaacgcaa aacggcaaaa tggcggcgtt tgcgattacg caagtggtgc
     2041 tggatgaagc tacattgttc aatattgcgg tcgatcctga ctatcagcgt cagggattgg
     2101 gaagggtgct gctggaacat ctgatcgacg aactggaaaa acgcggcgtg gcgacactat
     2161 ggctggaagt ccgtgcctca aatgctgccg ccattgccct gtacgaaagt ttaggcttta
     2221 acgaggcgac gattcgccgc aattactacc ccaccacgga cggtcgcgaa gacgccatca
     2281 tcatggcgtt gccaatcagt atgtaagaca aggtggaata atgaagtggg actggatttt
     2341 ctttgatgcc gatgaaacgc tgtttacctt tgactcattc accggcctgc agcggatgtt
     2401 tcttgattac agcgtcacct ttaccgctga agattttcag gactatcagg ccgttaacaa
     2461 gccactgtgg gtggattatc aaaacggcgc gatcacttca ttacagcttc agcacgggcg
     2521 ttttgagagc tgggccgaac ggctgaaagt tgaagcaggc ttgcttaacg atgcctttat
     2581 taatgcgatg gcggaaatct gcacgccgct gccgggcgcg gtttctctgc ttaacgccat
     2641 tcgtggcaac gccaaaatcg gcatcatcac caacggcttt agcgccttgc agcaagtgcg
     2701 tctggaacgc acgggcctgc gtgattactt cgatttgctg gtgatttccg aagaagttgg
     2761 cgttgccaaa ccgaataaga aaattttcga ttatgcgctg gaacaggcgg gcaatcctga
     2821 ccgttcacgc gtgctgatgg ttggcgacac tgccgagtcc gatattctcg gtggcatcaa
     2881 cgccgggctt gcgacctgct ggctgaatgc gcacaatcgc gagcaaccag aaggcatcgc
     2941 gcccacctgg accgtttctt cgttgcacga actggagcag ctcctgtgta aacactgatt
     3001 gcctcccccc cgttgatggg taaaatagcc gcaatttttc gttttcaaca agcgcggcgc
     3061 gatgccgctt actcaagaag aaagaattat gacgttgtct ccttatttgc aagaggtggc
     3121 gaagcgccgc acttttgcca ttatttctca cccggatgcc ggtaagacta ccatcaccga
     3181 gaaggtgctg ctgttcggac aggccattca gaccgccggt acagtaaaag gccgtggttc
     3241 caaccagcac gctaagtcgg actggatgga gatggaaaag cagcgtggga tctccattac
     3301 tacgtctgtg atgcagtttc cgtatcacga ttgcctggtt aacctgctcg acaccccggg
     3361 gcacgaagac ttctcggaag atacctatcg taccctgacg gcggtggact gctgcctgat
     3421 ggttatcgac gctgcaaaag gtgttgaaga tcgtacccgt aagctgatgg aagttacccg
     3481 tctgcgcgac acgccgatcc tcacctttat gaacaaactt gaccgtgata tccgcgaccc
     3541 gatggaactg ctcgatgaag ttgagaacga gctgaaaatc ggctgcgcac cgatcacctg
     3601 gccgattggc tgcggcaagc tgtttaaagg cgtttaccac ctttataaag acgaaaccta
     3661 tctctatcag agcggtaaag gccacacgat tcaggaagtc cgtattgtta aagggctgaa
     3721 taacccggat ctcgacgctg cggttggtga agatctggca cagcagctac gtgacgaact
     3781 ggaactggtg aaaggcgcgt ctaacgagtt cgacaaagaa ttgttccttg cgggcgaaat
     3841 cactccggta ttcttcggta ctgcgctggg taacttcggc gttgatcata tgctggatgg
     3901 cctggtggag tgggctcctg cgccgatgcc gcgtcagact gatacccgta ccgtagaggc
     3961 gagcgaagac aaatttaccg gcttcgtatt taaaattcag gccaacatgg acccgaaaca
     4021 ccgcgaccgc gtggcgttta tgcgcgtagt atccggtaaa tatgaaaaag gcatgaagct
     4081 gcgccaggtg cgtactgcga aagatgtggt tatctccgac gcgctgacct ttatggcggg
     4141 cgaccgttcg cacgttgaag aagcgtatcc gggcgatatc ctcggcctgc acaaccacgg
     4201 cactattcag atcggcgaca cctttaccca gggtgagatg atgaagttca ccggtattcc
     4261 gaacttcgca ccagaactgt tccgtcgtat ccgcctgaaa gatccgctga agcaaaaaca
     4321 gctgctcaaa gggctggtac agctttccga agagggcgcg gtgcaggtgt tccgtccgat
     4381 ctccaacaac gatttgatcg ttggtgctgt tggtgtgctg cagtttgatg tggtggtatc
     4441 gcgcctgaag agcgaataca gcgttgaagc agtatatgaa tcagtcaacg ttgccactgc
     4501 ccgctgggta gaatgtgcgg acgcgaagaa attcgaagag ttcaagcgta agaacgaaag
     4561 ccaactggcg cttgatggcg gcgataacct cgcttacatc gctaccagca tggtcaacct
     4621 gcgcctggcg caggaacgtt atcccgacgt tcagttccac cagacccgcg agcattaatt
     4681 cttgcctcca gggcgcggta gccgctgcgc cctgtcaatt tcccttcctt attagccgct
     4741 tacggaatgt tcttaaaaca ttcacttttg cttatgtttt cgctgatatc ccgagcggtt
     4801 tcaaaattgt gatctatatt taacaaagta atgacatttc tgacggcgtt aaataccgtt
     4861 caatgcgtag atatcagtat ctaaagccgt cgattgtcat tctaccgata ttaataactg
     4921 attcagaggc tgtaatggtc gttattcatc acccatcgct tttgtgatgg cgaccattga
     4981 cttctgtaga gggtgaagtc tctccctatt cagcaatgca acctcgtgtt gccaggctca
     5041 aattacgagc aaacatacag gaataaatcg atgactatga caagactgaa gatttcgaaa
     5101 actctgctgg ctgtaatgtt gacctctgcc gtcgcgaccg gctctgccta cgcggaaaac
     5161 aacgcacaga ctaccaatga aagcgcaggg caaaaagtcg atagctctat gaataaagtc
     5221 ggtaatttca tggatgacag cgccatcacc gcgaaagtga aggcggcgct ggtggatcat
     5281 gacaacatca agagcaccga tatctctgtc aaaaccgatc aaaaagtcgt gaccctgagc
     5341 ggtttcgttg aaagccaggc tcaggccgaa gaggcagtga aagtggcgaa aggcgttgaa
     5401 ggggtgacct ctgtcagcga caaactgcac gttcgcgacg ctaaagaagg ctctgtgaag
     5461 ggctacgcgg gtgacaccgc caccaccagt gaaatcaaag ccaaactgct ggcggacgat
     5521 atcgtccctt cccgtcatgt gaaagttgaa accaccgacg gcgtggttca gctctccggt
     5581 accgtcgatt cccaggcaca gagcgaccgt gctgaaagta tcgccaaagc ggtagatggt
     5641 gtgaaaagcg ttaaaaatga tctgaaaact aagtaattcg tcgtaattcg tcctcccgaa
     5701 ctttgtcggg aggcgcgatg tgcaccacac taaaaatatc gcattgagtg attttcacgc
     5761 tcatatcaag cggtgacatt aactatggta aaggagacgc ttatgtttcg ttggggcatc
     5821 atatttctgg ttatcgcgtt aatcgccgcc gcacttgggt ttggtggtct ggccggtacc
     5881 gctgcaggcg cagctaaaat tgtctttgtc gtcgggatta ttctgttcct ggtgagtttg
     5941 ttcatgggcc gaaaacgacc ctagatttca gaccattaag aaaattttat ccaaagccag
     6001 tccagcggac tggcttttgc ggttttagcg aataataaat tgcgttactt tgtcattctt
     6061 tcacaacaaa aacaggaagg cagaggtggg gcagcgaata cctgtaacgc ttggtaatat
     6121 tgcgccgttg tcgctaaggc cgttccagcc tggacgaata gctctggtgt gcgaaggcgg
     6181 cggacagcgt ggaattttca cggctggcgt gctggatgag tttatgcgcg cgcagtttaa
     6241 tcctttcgat ctttatctcg gcacatctgc cggggcgcag aacctctcgg cgtttatctg
     6301 caatcagccc ggttacgcgc gcaaagtcat catgcgctat accacaaaac gcgaattttt
     6361 cgatccattg cgctttgtcc gtggaggaaa tcttatcgat ctcgactggc tggtggaggc
     6421 caccgcaagc cagatgccgt tgcaaatgga taccgccgcg cggttgtttg acagcggcaa
     6481 atcgttttat atgtgcgcct gtcgtcagga tgactacgcg cctaattact ttttaccaac
     6541 caaacaaaac tggctggatg tgattcgcgc ctccagtgcg atacctggct tttatcgtag
     6601 cggagtgtcg ctggaaggca ttaactacct ggatggcggg atcagtgatg cgattccggt
     6661 taaagaggcg gcaaggcagg gcgctaaaac gttggtcgtc attcgcactg tgccgtcaca
     6721 aatgtactac acgccgcagt ggttcaaacg catggaacgc tggctgggtg acagtagcct
     6781 gcagccgctg gtcaatctgg tgcagcatca tgaaaccagc tatcgtgaca ttcagcaatt
     6841 tattgagaaa ccaccgggca agctgcggat attcgaaatt tatccgccga agccattaca
     6901 tagtatcgcg cttggcagtc ggattccggc gctgcgtgaa gactataaac ttgggcgttt
     6961 atgcggtcgt tatttcctcg ccacggttgg caagctatta actgaaaaag cgccgcttac
     7021 ccgccatctg gtgccagtgg tgacgccgga atcgatcgtt attccgcctg cgccagtcgc
     7081 caacgatacg ctggttgccg aagtgagcga tgctccgcag gcgaacgacc cgacatttaa
     7141 caatgaggat ctggcttgat ttgccgtttt atcgataccc actgccattt tgatttcccg
     7201 ccgtttagtg gcgatgaaga ggccagcctg caacgcgcgg cacaagcggg cgtaggcaag
     7261 atcattgttc cggcgacaga ggcggaaaat tttgcccgcg tgcaggcgtt agcggaaaaa
     7321 tatcaaccgc tgtatgccgc attggggttg catccgggga tgttggaaaa acatagcgat
     7381 gtgtctcttg atcagctaca gcaggcgctg gaaaggcgtc cggcgaaggt ggtggcggtg
     7441 ggggagatcg gtctggatct cttcggcgac gatccgcaat ttgagaggca gcagtggtta
     7501 ctcgacgaac agctgaaact ggcgaaacgc tacgatctgc cggtgatcct tcattcccgg
     7561 cgtatacacg acaaactggc gatgcatctt aaacgtcacg atttaccgtg cactggcgtg
     7621 gttcacggtt tttccggcag cctgcaacag gccgagcggt ttgtacagct gggctacaaa
     7681 atcggcgtag gcggtactat cacctatcca cgcgccagta aaacccgcga tgtcatcgca
     7741 aaattaccgc tggcatcgtt gttgctggaa accgacgcgc cggatatgcc gctcaacggc
     7801 tttcaggggc agcctaaccg accggaacag gctgtgcggg tgtttgacgt gctttgcgag
     7861 ttgcgcccgg aaccagaaga tgagattgcc gaagtgttgc ttaataatac gtatgcgttg
     7921 tttagcgtta gtgggtagcc tacccttcgt aggcagcatc cggcagtcgg cgcataatgc
     7981 ctgatgcgac gctt
//
LOCUS       DAZOZY010000184         7990 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZOZY010000184 DAZOZY010000000
VERSION     DAZOZY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.185"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            128..739
                     /gene="ruvA"
                     /locus_tag="ACHURQ_003438"
     CDS             128..739
                     /gene="ruvA"
                     /locus_tag="ACHURQ_003438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR0825145.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            748..1758
                     /gene="ruvB"
                     /locus_tag="ACHURQ_003439"
     CDS             748..1758
                     /gene="ruvB"
                     /locus_tag="ACHURQ_003439"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR0825146.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1905..2690)
                     /gene="znuB"
                     /locus_tag="ACHURQ_003440"
     CDS             complement(1905..2690)
                     /gene="znuB"
                     /locus_tag="ACHURQ_003440"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR0825147.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2687..3442)
                     /gene="znuC"
                     /locus_tag="ACHURQ_003441"
     CDS             complement(2687..3442)
                     /gene="znuC"
                     /locus_tag="ACHURQ_003441"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR0825148.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3521..4453
                     /gene="znuA"
                     /locus_tag="ACHURQ_003442"
     CDS             3521..4453
                     /gene="znuA"
                     /locus_tag="ACHURQ_003442"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR0825149.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4469..5791
                     /gene="mepM"
                     /locus_tag="ACHURQ_003443"
     CDS             4469..5791
                     /gene="mepM"
                     /locus_tag="ACHURQ_003443"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR0825150.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5911..6882
                     /gene="lpxM"
                     /locus_tag="ACHURQ_003444"
                     /gene_synonym="msbB"
     CDS             5911..6882
                     /gene="lpxM"
                     /locus_tag="ACHURQ_003444"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR0825151.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6970..7990)
                     /locus_tag="ACHURQ_003445"
                     /pseudo
     CDS             complement(6970..7990)
                     /locus_tag="ACHURQ_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2128 a         1915 c         2014 g         1933 t
ORIGIN      
        1 aattatactg tgccattttt cagttcatcg agacacctcg caagttttct tcatccttcg
       61 ctggatatct atccagcatt tttttatcat acagcattat ctttgattca ttacgcagga
      121 gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc cgctggtgtt
      181 aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt tttatgaact
      241 ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg aagacgcgca
      301 actgctgtac ggttttaaca ataaacaaga gcgcacgttg tttaaagagt taatcaaaac
      361 caacggcgtc ggcccgaagt tggcgctggc gatcctctcc ggaatgtcag cgcagcagtt
      421 cgttaatgcc gttgaacgtg aagaagtggg agcactggtg aaactgccgg gcattggtaa
      481 aaaaaccgcc gagcgtttaa ttgtcgaaat gaaagaccga tttaaaggtt tgcacggcga
      541 tctctttacg ccagccgccg atctggtgct cacgtcgcct gccagcccgg caaccgacga
      601 tgctgaacag gaagcggttg ccgcgctggt ggcgctgggc tataaaccac aagaagcaag
      661 ccgcatggtg agcaaaatcg ctcgcactga cgccagcagt gaaacattaa ttcgcgaagc
      721 cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat ttctgccggt
      781 accactttgc cggaagatgg ggcagatcgc gccattcgcc ctaaattact ggaagagtat
      841 gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc gaaactgcgc
      901 ggcgatgccc tcgatcatct gttgattttt ggtcctccgg ggttgggtaa aactacgctt
      961 gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg tccggtgctg
     1021 gaaaaggcgg gcgatctggc agcgatgctc actaaccttg aaccgcatga cgtgctgttt
     1081 attgatgaga tccaccgtct atcgccagtt gttgaagaag tgctgtaccc ggcaatggaa
     1141 gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat taaaattgat
     1201 ttgccgccgt ttaccctgat tggtgcaacc acgcgcgcag gttcgctgac atcaccgttg
     1261 cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga tctgcaatat
     1321 atcgtcagtc gcagcgcacg ctttatgggg cttgagatga gtgatgacgg cgcgctggaa
     1381 gttgctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg tcgagtgcgt
     1441 gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc tcaggcgctg
     1501 gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt gttgctggcg
     1561 gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc agccattggc
     1621 gaagaacgtg aaaccattga ggatgtgctg gagccttatc tgattcagca aggctttttg
     1681 cagcgtacac cgcgtgggcg tatggcgacg acgcgggcgt ggaatcactt tggcataacg
     1741 ccgccagaaa tgccgtaagt cggattggtc gctcgcaccg catccgacat tggcagtgtg
     1801 cctgatgcga cgctattcgc gtcttatccg gcctaccaga gatagcgaac cgtaggtcgg
     1861 ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga cagattagct ggcctgcttt
     1921 ttcatcatac tgataataaa taacagtgca gcacatagca ctaccgacgg acccgccggc
     1981 gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc caccaaaaca
     2041 gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc tgcaggaata
     2101 atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc aatcgtcaat
     2161 gccgtcacca gcatcaacaa caatttcacg cgctgtaatt tcacaccatc aacaaacgcc
     2221 agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa aatagccacc
     2281 acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc cagcaaatca
     2341 ccgaacaggt aagccatcaa atcaacacga atattagaca tcagactaac gaccaccagg
     2401 cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc cagctgtgga
     2461 cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat caccgcatag
     2521 aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc atgagccagc
     2581 gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg acccgcggca
     2641 caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat gagcgatcat
     2701 ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atggttatga tgatggcgat
     2761 agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc ggatgcaggg
     2821 aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc acttcatcgg
     2881 tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa tccagttcac
     2941 gacgcagttg gtcaataagg tcatataacg ccacctggcc atttacatcc acgccttgag
     3001 tgggttcatc cagcactaat aattgcgggc gatttaacaa tgctcgcgct aacagtacac
     3061 gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc ccggcctgga
     3121 cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag cgtaaaaaac
     3181 ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca tagccgatgc
     3241 gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg agcactaccc
     3301 gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt tttccaggtt
     3361 taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc gagacatttt
     3421 ccaaggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga aatgttataa
     3481 tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata aaaaaacgct
     3541 tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg cagatgccgc
     3601 cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg atggggtaac
     3661 agaaacagag gttttacttc ctgacggcgc ttcagaacat gattattcac tgcgcccatc
     3721 ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg agatggaagc
     3781 gtttatgcaa aaaccggtaa gcaaattacc aggagcgaag caggtaacga ttgcgcagct
     3841 tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg atcacgacca
     3901 cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc tttggctttc
     3961 cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg aacttatgcc
     4021 gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat tagcctcaac
     4081 cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt tcgtttttca
     4141 cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc attttaccgt
     4201 taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac agttggttga
     4261 gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg ttgagagcgt
     4321 cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga atatcaaact
     4381 gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg cgagctgcct
     4441 gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg tcgccctggc
     4501 gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg ttcttactct
     4561 ggccgtcgct gtctggcggc cctatgttta tcaccgcgat gccacgccaa ttgtcaaaac
     4621 cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg agccgattga
     4681 tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca aaatcgccgg
     4741 tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca gcattctcaa
     4801 tcagtatggt attgatatgg gtgatatcac ccaactggct gcggccgaca aagaattgcg
     4861 taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg aactgcagcg
     4921 cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg ccgctaacgg
     4981 ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc tgaaaggtac
     5041 cgtcgggggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg ccgaagtgag
     5101 cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa aaggcgatga
     5161 atttgcggtg ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa gccagctgct
     5221 gggcgtacgt ttgcgttcag aaggtaaaga ttattacgct atccgcgctg aagatggcaa
     5281 attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc cgacggcgaa
     5341 acagttccgt atctcgtcta actttaaccc gcgtcgtact aatccggtga ccggtcgcgt
     5401 tgcagcacac agaggtgttg atttcgccat gccgcagggt acgccagtgc tttcagtggg
     5461 tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg tggctattcg
     5521 tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg tgaaaccggg
     5581 acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac gttcaaccgg
     5641 gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc tgacggcaaa
     5701 actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg cgcaggccaa
     5761 agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc ggtacgcagt
     5821 cagtatcggc tttttttatt tggtgcgggg caagttgcgc cgctacacta tcaccagatt
     5881 gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa tagcgaatac
     5941 attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg gctgggtgta
     6001 gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat tctggcacgg
     6061 ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt aatcaatctg
     6121 tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga tgagatgttt
     6181 gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg accggagaaa
     6241 attcagccgc gcgttgactg gcaagggctg gagatcatcg aagagatgag gcgtaataac
     6301 gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc catgctgatg
     6361 gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc ggtttttgat
     6421 tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag aaatgacggt
     6481 attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt acccgatcag
     6541 gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa agcgacgttg
     6601 cccgcgattg gtcgtttgat gaaagtgtgc cgtgcgcgcg ttgttccgct gtttccgatt
     6661 tatgatggca agacgcaccg tctgacgatt caggtgcgcc caccgatgga tgatctgtta
     6721 gaggcggatg atcatacgat tgcgcggcgg atgaatgaag aagtcgagat ttttgttggt
     6781 ccgcgaccag aacaatacac ctggatatta aaattgctga aaactcgcaa accgggcgaa
     6841 atccaaccgt ataagcgtaa agatctttat cctatcaaat aaaaaaagcc tctcaaatta
     6901 ttgagaggct gtactcgata aaaaatctca atgagtgttc ccactatttt gagtttttag
     6961 gttagtacgc tattgagtta atcgtagcgt gataatttca aaaggtttga actcaagatt
     7021 gcaatattga ttgcaataaa aactgtcatc taacttattt tcttgaagat cgacatattg
     7081 aacgtagtga taattacata ctcacgaaag catggctgaa atttctaatc catgactctc
     7141 atataatctt acgattatac cgtcgccgtc ttcagctaat tttattgttt cgatgataac
     7201 gctgttagta tcgatagcaa aaaatgatag tgaagggggt aattcacccg ggtgcggttt
     7261 gacagggacg acctgtaagg gatgattcat tatataggct tgattaataa caccacccac
     7321 acggtaatct ccatgatgag gaaataagga gaatatgaat ttttgtttac caatatctgc
     7381 aaaattatta atcaggaaat tatcataatt gtctgtctta acaacttccc caggtttatg
     7441 ctgcgaacgc agcaaagtta attcaagcgt ctggtctttg acacgatagc catatttgtt
     7501 ttttgctatt aaagctatgc cagtttctcg atcagaaagg tcgacccagt gatgtgctgg
     7561 tatttcatct ttggcaaaag cgaacgaatc atcgctatgc gttggtctgt caattacacc
     7621 gaactgaatt tgacattgtg caagagatgt ttgtactgac accgggaatt ttactttgag
     7681 agaaacatta ggttcgtgcc aatcaataaa cgtttggaca tcaacgcgtg gactatcctg
     7741 tgtcagggta aaggctaaac gaagataaga ggaattgtag tgataaatca gcgtcatcgt
     7801 agttttaggc ccgctgatca cagttgtcat tttttcagcg agtaaaacat atgattcccc
     7861 atcacgataa tttgaagcaa aatcccaggc cgcgaaaaaa cctgcatctt cgtggtatgc
     7921 acggatctca ctgtgcatat tttcagtaat gtattcttta ccatatcttt tatcatatag
     7981 cgaaattaaa
//
LOCUS       DAZOZY010000185         7973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZOZY010000185 DAZOZY010000000
VERSION     DAZOZY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.186"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHURQ_003446"
     CDS             <1..643
                     /locus_tag="ACHURQ_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0825152.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHURQ_003447"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHURQ_003447"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR0825153.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHURQ_003448"
     CDS             2474..2986
                     /locus_tag="ACHURQ_003448"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR0825154.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHURQ_003449"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHURQ_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR0825155.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHURQ_003450"
     CDS             5579..5782
                     /locus_tag="ACHURQ_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR0825156.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHURQ_003451"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHURQ_003451"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR0825157.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..7723)
                     /locus_tag="ACHURQ_003452"
                     /pseudo
     CDS             complement(6827..7723)
                     /locus_tag="ACHURQ_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT         1833 a         2173 c         2097 g         1870 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcgatctgc cccgttactt tattcgtacc
     7741 ccttataatg gggtgttagc cagccagacc cggcatgatt actgccccca gtcgtccatg
     7801 atccgggggg tgatgtcacc gggtctggtg gggcgctggt aaccgctaat aggggtcagg
     7861 tcaggcactt ttgccgggac cgtctgtaac gtggatgccg gtacctgctc cccgtggtta
     7921 tctggttaac ccatatacaa gggagacaga atgaccgaat ccagcgatta cga
//
LOCUS       DAZOZY010000186         7948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZOZY010000186 DAZOZY010000000
VERSION     DAZOZY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.187"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHURQ_003453"
     CDS             complement(152..487)
                     /locus_tag="ACHURQ_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR0825158.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHURQ_003454"
     CDS             complement(491..835)
                     /locus_tag="ACHURQ_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR0825159.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHURQ_003455"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHURQ_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR0825160.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHURQ_003456"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHURQ_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR0825161.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHURQ_003457"
     CDS             1257..1376
                     /locus_tag="ACHURQ_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0825162.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHURQ_003458"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHURQ_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR0825163.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHURQ_003459"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHURQ_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR0825164.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHURQ_003460"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHURQ_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR0825165.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHURQ_003461"
     CDS             4176..4544
                     /locus_tag="ACHURQ_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR0825166.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHURQ_003462"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHURQ_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR0825167.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHURQ_003463"
     CDS             5295..5588
                     /locus_tag="ACHURQ_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR0825168.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHURQ_003464"
     CDS             5660..6319
                     /locus_tag="ACHURQ_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR0825169.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHURQ_003465"
     CDS             6411..6857
                     /locus_tag="ACHURQ_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR0825170.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..>7948)
                     /gene="hlyE"
                     /locus_tag="ACHURQ_003466"
     CDS             complement(7064..>7948)
                     /gene="hlyE"
                     /locus_tag="ACHURQ_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR0825171.1"
                     /translation="VEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQE
                     YSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNEKKASA
                     QKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQ
                     VDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFFTTLSN
                     TVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTC
                     NEYQKKHGKKTLFEVPEV"
BASE COUNT         2045 a         1785 c         1833 g         2285 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctac
//
LOCUS       DAZOZY010000187         7875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZOZY010000187 DAZOZY010000000
VERSION     DAZOZY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.188"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            115..519
                     /gene="ybgC"
                     /locus_tag="ACHURQ_003467"
     CDS             115..519
                     /gene="ybgC"
                     /locus_tag="ACHURQ_003467"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR0825172.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            516..1208
                     /gene="tolQ"
                     /locus_tag="ACHURQ_003468"
     CDS             516..1208
                     /gene="tolQ"
                     /locus_tag="ACHURQ_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR0825173.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            1212..1640
                     /gene="tolR"
                     /locus_tag="ACHURQ_003469"
     CDS             1212..1640
                     /gene="tolR"
                     /locus_tag="ACHURQ_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR0825174.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            1705..2937
                     /gene="tolA"
                     /locus_tag="ACHURQ_003470"
     CDS             1705..2937
                     /gene="tolA"
                     /locus_tag="ACHURQ_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR0825175.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            3070..4362
                     /gene="tolB"
                     /locus_tag="ACHURQ_003471"
     CDS             3070..4362
                     /gene="tolB"
                     /locus_tag="ACHURQ_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR0825176.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            4397..4918
                     /gene="pal"
                     /locus_tag="ACHURQ_003472"
     CDS             4397..4918
                     /gene="pal"
                     /locus_tag="ACHURQ_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR0825177.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            4928..5719
                     /gene="cpoB"
                     /locus_tag="ACHURQ_003473"
     CDS             4928..5719
                     /gene="cpoB"
                     /locus_tag="ACHURQ_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR0825178.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            5884..5959
                     /locus_tag="ACHURQ_003474"
     tRNA            5884..5959
                     /locus_tag="ACHURQ_003474"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5917..5919,aa:Lys,seq:ttt)
     gene            6109..6184
                     /locus_tag="ACHURQ_003475"
     tRNA            6109..6184
                     /locus_tag="ACHURQ_003475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6142..6144,aa:Val,seq:tac)
     gene            6188..6263
                     /locus_tag="ACHURQ_003476"
     tRNA            6188..6263
                     /locus_tag="ACHURQ_003476"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6221..6223,aa:Lys,seq:ttt)
     gene            6410..6485
                     /locus_tag="ACHURQ_003477"
     tRNA            6410..6485
                     /locus_tag="ACHURQ_003477"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6443..6445,aa:Lys,seq:ttt)
     gene            6519..6594
                     /locus_tag="ACHURQ_003478"
     tRNA            6519..6594
                     /locus_tag="ACHURQ_003478"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6552..6554,aa:Lys,seq:ttt)
     gene            6619..6750
                     /locus_tag="ACHURQ_003479"
     ncRNA           6619..6750
                     /ncRNA_class="other"
                     /locus_tag="ACHURQ_003479"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            6999..>7875
                     /gene="nadA"
                     /locus_tag="ACHURQ_003480"
     CDS             6999..>7875
                     /gene="nadA"
                     /locus_tag="ACHURQ_003480"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR0825179.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         2098 a         1887 c         2104 g         1786 t
ORIGIN      
        1 aggcccataa atttttacgc tcccttaact tgccctcatt cccaaacctc aatcgcgcgc
       61 gtatagtagc agcgtttaaa agctctaact tttgttgcat taccgggatg taaagtgaat
      121 acaacgctgt ttcgatggcc ggttcgcgtc tactatgaag ataccgatgc cggtggtgtg
      181 gtgtaccacg ccagttacgt cgctttttat gaaagagcac gcacagagat gctgcgtcat
      241 catcacttca gtcagcaggc gctgatggct gaacgcgttg cctttgtggt acgtaaaatg
      301 acggtggaat attacgcacc tgcgcggctc gacgatatgc tcgaaataca gactgaaata
      361 acatcaatgc gtggcacctc tttggttttc acgcaacgta ttgtcaacgc cgagaatact
      421 ttgctgaatg aagcagaggt tctggttgtt tgcgttgacc cactcaaaat gaagcctcgt
      481 gcgcttccca agtctattgt cgcggagttt aagcagtgac tgacatgaat atccttgatt
      541 tgttcctgaa ggctagcctt ctggttaaac ttatcatgtt gattttgatt ggtttttcaa
      601 tcgcatcctg ggccattatt atccagcgga cccgtattct taacgcagcg gcgcgcgaag
      661 ccgaagcgtt tgaagataaa ttctggtctg gaatcgaact ctctcgcctc tatcaagaga
      721 gtcaggggaa acgggataat ctgactggtt cggaacaaat attttacagc gggttcaaag
      781 agtttgtgcg cctgcatcgt gccaatagcc atgcgccgga agccgtagtg gaaggggcgt
      841 cgcgtgctat gcgtatctcc atgaaccgtg aacttgaaaa tctggaaacg cacattccgt
      901 tcctcggtac ggttggctcc atcagcccgt atattggtct gtttggtacg gtctggggga
      961 tcatgcacgc ctttatcgcc ctcggggcgg taaaacaagc aacactgcaa atggttgcgc
     1021 ccggtatcgc agaagcgttg attgcgactg caattggtct gttcgccgca attccggcgg
     1081 taatggctta taaccgcctc aaccagcgcg taaacaaact ggaactgaat tacgacaact
     1141 ttatggaaga gtttaccgcg attctgcacc gccaggcgtt taccgttagc gagagcaaca
     1201 aggggtaagc catggccaga gcgcgtggac gaggtcgtcg cgatctcaag tccgaaatca
     1261 acattgtacc gttgctggac gtactgctgg tgctgttgct gatctttatg gcgacagcgc
     1321 ccatcatcac ccagagcgtg gaggtcgatc tgccagacgc tactgaatca caggcggtga
     1381 gcagtaacga taatccgcca gtgattgttg aagtgtctgg tattggtcag tacaccgtgg
     1441 tggttgagaa agatcgcctg gagcgtttac caccagagca ggtggtggcg gaagtgtcca
     1501 gccgtttcaa ggccaacccg aaaacggtct ttctgatcgg tggcgcaaaa gatgtgcctt
     1561 acgatgaaat aattaaagca ctgaacttgt tacatagtgc gggtgtgaaa tcggttggtt
     1621 taatgacgca gcctatctaa acatctgcgt ttcccttgct tgaaagagag cgggtaacag
     1681 gcgaacagtt tttggaaacc gagagtgtca aaggcaaccg aacaaaacga caagctcaag
     1741 cgggcgataa ttatttcagc agtgctgcat gtcatcttat ttgcggcgct gctctggagt
     1801 tcgttcgatg agaatataga agcttcagcc ggaggcggcg gtggttcgtc catcgacgct
     1861 gtcatggttg attcaggtgc ggtagttgag cagtacaaac gcatgcaaag ccaggaatca
     1921 agcgcgaagc gttctgatga gcagcgcaag ataaaggaac agcaggctgc tgaagaactg
     1981 cgtgagaaac aagcggctga acaggaacgc ctgaagcaac ttgagaaaga gcggttagct
     2041 gctcaggaac agaaaaagca ggctgaagaa gccgcaaaac aggccgagtt aaagcagaag
     2101 caagcggaag aggcggcagc gaaagcggcg gcagatgcta aagcgaaggc cgaagcggat
     2161 gctaaagctg cggaagaagc agcgaagaaa gcggcggcag acgcgaagaa aaaagcagaa
     2221 gcagaagccg ccaaagccgc agccgaagcg cagaaaaaag ccgaggcagc cgctgcggca
     2281 ctgaagaaga aagcggaagc ggcagaagct gctgcagctg aagcaagaaa gaaagcggca
     2341 gcagagaaag ctgcagccga caaaaaagca gcagaaaaag cggctgctga taaggcagca
     2401 gctgataaga aagcagcggc agaaaaagcc gccgcagaca aaaaagcggc agcggcaaaa
     2461 gcagcggcag aaaaagccgc cgcagacaaa aaagcggcag cggcaaaagc agcagctgaa
     2521 aaagccgctg cagcaaaagc tgccgcagag gcagatgata ttttcggtga gctaagctct
     2581 ggtaagaatg caccgaaaac ggggggaggg gcgaaaggga acaatgcttc gcctgccggg
     2641 agtggtaata ctaaaaacaa tggcgcatca ggggccgata tcaataacta tgccgggcag
     2701 attaaatctg ctatcgaaag taagttctat gacgcatcgt cctatgcagg caaaacctgt
     2761 acgctgcgca taaaactggc acccgatggt atgttactgg atatcaaacc tgaaggtggc
     2821 gatcccgcac tttgtcaggc tgcgttggca gcagctaaac ttgcgaagat cccgaaacca
     2881 ccaagccagg cagtatatga agtgttcaaa aacgcgccat tggacttcaa accgtaatcg
     2941 cgatgttgac tgttccgacg gtcaacatca ggcaccggtt gccacggggt tctggtagtt
     3001 ttgtgtattt tagtttgtta acattctgct aaattatcgt gggccatcgg tccagataag
     3061 ggagatatga tgaagcaggc attacgagta gcatttggtt ttctcatact gtgggcatca
     3121 gttctgcatg ctgaagtccg cattgtgatc gacagcggtg tagattccgg tcgtcctatt
     3181 ggtgttgttc ctttccagtg ggcgggccct ggtgcggcac ctgaagatat tggcggcatc
     3241 gttgctgccg acttgcgtaa cagcggtaaa tttaatccgt tagatcgcgc tcgtctgcca
     3301 cagcagccgg gtagtgcgca ggaagtacaa ccagctgcat ggtccgcgct gggtattgac
     3361 gctgtagttg tcggtcaggt cactccgaat ccggatggtt cttacaatgt tgcttatcaa
     3421 cttgttgaca ctggcggcgc accgggtact gtacttgctc agaactcgta caaagtgaac
     3481 aagcagtggc tgcgctatgc tggtcatacc gccagtgatg aagtgtttga aaaactgacc
     3541 ggcattaaag gtgcgttccg tacccgtatt gcctacgttg ttcagaccaa cggcggtcag
     3601 ttcccgtatg aactgcgcgt atctgactat gacggttaca accagtttgt cgttcaccgt
     3661 tcaccacagc cgctgatgtc cccggcgtgg tcaccagacg gttctaaact ggcttatgtg
     3721 accttcgaaa gcggtcgttc cgcgctagtt atccaaacgc tggcaaacgg cgctgtacgt
     3781 caggtggctt cattcccgcg tcacaacggt gcgcctgcat tctcgccaga cggcagcaaa
     3841 ctggcattcg ccttgtcgaa aaccggtagc ctgaacctgt acgtaatgga tttggcttct
     3901 ggtcagatcc gccaggtgac tgatggtcgc agtaacaata ccgaaccgac ctggttcccg
     3961 gacagccaga acctggcatt tacttctgac caggccggtc gtccacaggt ttataaagtg
     4021 aatatcaacg gcggtgcgcc acaacgtatt acctgggaag gttcgcagaa ccaggatgcg
     4081 gatgtcagca gcgacggtaa atttatggta atggtcagct ccaacggtgg gcagcagcac
     4141 attgccaaac aagatctggc aacgggaggc gtacaagttc tgtcgtccac gttcctggat
     4201 gaaacgccaa gtctggcacc taacggcact atggtaatct acagctcttc tcaggggatg
     4261 ggatccgtgc tgaatttggt ttctacagat gggcgtttca aagcgcgtct tccggcaact
     4321 gatggacagg tcaaattccc tgcctggtcg ccgtatctgt gataataatt aattgaatag
     4381 taaaggaatc attgaaatgc aactgaacaa agtgctgaaa gggctgatga ttgctctgcc
     4441 tgttatggca attgcggcat gttcttccaa caagaacgcc agcaatgacg gcagcgaagg
     4501 catgctgggt gccggcactg gtatggatgc gaacggcggc aacggcaaca tgtcttccga
     4561 agagcaggct cgtctgcaaa tgcaacagct gcagcagaac aacatcgttt acttcgatct
     4621 ggacaagtac gatatccgtt ctgacttcgc tcaaatgctg gatgcacatg caaacttcct
     4681 gcgtagcaac ccgtcttaca aagtcaccgt agaaggtcac gcggacgaac gtggtactcc
     4741 ggaatacaac atctccctgg gtgaacgtcg tgcgaacgcc gttaagatgt acctgcaggg
     4801 taaaggcgtt tctgcagacc agatctccat cgtttcttac ggtaaagaaa aacctgcagt
     4861 actgggtcat gacgaagcgg catactccaa aaaccgtcgt gcggtactgg tttactaaga
     4921 gaattgcatg agcagtaact tcagacatca actattgagt ctgtcgttac tggttggtat
     4981 agcggccccc tgggccgctt ttgctcaggc accaatcagt agtgtcggct caggctcggt
     5041 cgaagaccgc gtcactcaac ttgagcgtat ttctaatgct cacagccagc ttttaaccca
     5101 actccagcaa caactttctg ataatcaatc cgatattgat tccctgcgtg gtcagattca
     5161 ggaaaatcag tatcaactga atcaggtcgt ggagcggcaa aagcagatcc tgttgcagat
     5221 cgacagcctc agcagcggtg gtgcagcggc gcaatcaacc agcagcgatc aaagcggtgc
     5281 ggcggcatca acgacgccga cagctgatgc tggtactgcg aatgctggcg cgccggtgaa
     5341 aagcggcgat gcaaacacgg attacaatgc agctattgcg ctggtgcagg ataaatcccg
     5401 ccaggatgac gcaatggtgg catttcagaa tttcatcaaa aattaccctg attcaactta
     5461 cctgccaaac gccaattatt ggctgggtca gttaaactac aacaagggta aaaaagatga
     5521 tgcggcgtac tattttgctt cggtagtgaa aaactatccg aagtcaccaa aggctgctga
     5581 tgcgatgttt aaagtcggcg tcatcatgca ggacaaaggt gacaccgcaa aagcgaaagc
     5641 cgtataccag caggttatca gtaaataccc tggtactgat ggcgctaaac aggcacaaaa
     5701 acgtctgaac gcgatgtaat gcataacaca cgaccagaag tcgcattatt tctggtcgtg
     5761 tcgtgcgaat cataagcagt tgagtgatct gcatcgaaat ttttgttgcg ctcaagtctg
     5821 aaatcagtaa tatatgccgc cgttgccacg ggatatcaaa caaaccgaaa gcaacgaaaa
     5881 agtgggtcgt tagctcagtt ggtagagcag ttgactttta atcaattggt cgcaggttcg
     5941 aatcctgcac gacccaccag ttttagcatc gaagacagat gttaagcgtg taggataacg
     6001 ttgcgtcagc aacggcccgt agggcgagcg aagcgagtca tcctgcacga cccaccacta
     6061 attacggtgg gttcggtgga agtagtttgt agtatccagc gcagtatcgg gtgattagct
     6121 cagctgggag agcacctccc ttacaaggag ggggtcggcg gttcgatccc gtcatcaccc
     6181 accactcggg tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg
     6241 ttcgaatcct gcacgaccca ccaattttaa catcaaactc agatgttaag cgtgaaggat
     6301 aacgttgcgc cagcaacggc ccgtagggca agcgaagcga gtcatcctgc acgacccacc
     6361 aatcttaaag attggccccg agtagaaatc tttcaggtaa cacccgtatg ggtcgttagc
     6421 tcagttggta gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc
     6481 caccatcctg aatgattaaa gcagcataat cccgcaaggg gtcgttagct cagttggtag
     6541 agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgtaa
     6601 aaaagcgccc taaaggcgct tttttgctat ctgagatatt caaagattcg aacctgcagc
     6661 aggttcgagt tgagcgcagc gaaacaacgg agccgctcgc ggcgacggcc cgaagggcga
     6721 gcgaagcgag tcatcctgca cgacccacca atgtaaaaaa gcgccctaaa ggcgcttttt
     6781 tgctattcag gcatcctcaa tttcactttg taaacctgat gacatcgtca gagcttactg
     6841 tgcaagcaac tctacgtcgg tggaattagg cgtaattcga gtgacttttc cccaccattc
     6901 gactatcttg tttagcatat aaaacaaatt acgccgatag tagcgaatat tacgctaatg
     6961 tcggttttaa cgttaagcct gtaaaacgag atggtaagat gagcgtgatg tttgatccag
     7021 acacggcgat ttatcctttc cccccgaagc cgacgccgtt aagcattgat gaaaaagcgt
     7081 attaccgcga gaagataaaa cgtctgctaa aagaacgtaa tgcggtgatg gttgcccact
     7141 actataccga ccccgaaatt caacaactgg cagaagaaac cggtggctgt atttctgatt
     7201 ctctggaaat ggcgcgcttc ggtgcaaagc atcccgcttc tacgttgtta gtcgctgggg
     7261 tgagatttat gggagaaacc gccaaaattc tcagtccgga aaaaacaatt ctgatgctga
     7321 cacttcaggc tgaatgttca ctggatctcg gctgccctgt tgaagaattt aacgcatttt
     7381 gcgatgccca tcccgatcat actgtcgtcg tctacgccaa cacttctgct gcggtaaaag
     7441 cgcgcgcaga ttgggtggta acttcaagca ttgccgtcga acttattgat catctggata
     7501 gttttggtga aaaaatcatc tgggcacccg acaaacatct ggggcgttac gtgcaaaaac
     7561 agacgggcgc agacatttta tgctggcagg gtgcctgtat tgtgcatgat gaatttaaga
     7621 ctcaggcgtt aacccgcttg caagaagaat acccggatgc tgccatactg gtgcatccag
     7681 aatcaccaca agctattgtc gatatggcgg atgcggtcgg ttccaccagt caactgatcg
     7741 ctgctgcgaa aacattgcca catcagcggc ttatcgtggc aaccgatcgg ggcattttct
     7801 acaaaatgca gcaggcggtg ccagataaag agttactgga agcaccaacc gcaggtgagg
     7861 gtgcaacctg ccgca
//
LOCUS       DAZOZY010000188         7837 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZOZY010000188 DAZOZY010000000
VERSION     DAZOZY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.189"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHURQ_003481"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHURQ_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR0825180.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHURQ_003482"
     tRNA            1230..1305
                     /locus_tag="ACHURQ_003482"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHURQ_003483"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHURQ_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0825181.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHURQ_003484"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHURQ_003484"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR0825182.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHURQ_003485"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHURQ_003485"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR0825183.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHURQ_003486"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHURQ_003486"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR0825184.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="ACHURQ_003487"
     CDS             complement(4868..5305)
                     /locus_tag="ACHURQ_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR0825185.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="ACHURQ_003488"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="ACHURQ_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHURQ_003489"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHURQ_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR0825186.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT         2200 a         2081 c         1665 g         1891 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca caaaaca
//
LOCUS       DAZOZY010000189         7774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZOZY010000189 DAZOZY010000000
VERSION     DAZOZY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.190"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHURQ_003490"
     CDS             complement(<1..1780)
                     /locus_tag="ACHURQ_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR0825187.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHURQ_003491"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHURQ_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR0825188.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHURQ_003492"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHURQ_003492"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR0825189.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHURQ_003493"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHURQ_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR0825190.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHURQ_003494"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHURQ_003494"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR0825191.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIHVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHURQ_003495"
     CDS             complement(5968..7005)
                     /locus_tag="ACHURQ_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR0825192.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHURQ_003496"
     CDS             7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHURQ_003496"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR0825193.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKY"
BASE COUNT         1977 a         2101 c         1872 g         1824 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacatg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacg
//
LOCUS       DAZOZY010000190         7642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZOZY010000190 DAZOZY010000000
VERSION     DAZOZY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.191"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(183..929)
                     /gene="cutC"
                     /locus_tag="ACHURQ_003497"
     CDS             complement(183..929)
                     /gene="cutC"
                     /locus_tag="ACHURQ_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR0825194.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(943..1509)
                     /locus_tag="ACHURQ_003498"
     CDS             complement(943..1509)
                     /locus_tag="ACHURQ_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR0825195.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1725..3461
                     /gene="argS"
                     /locus_tag="ACHURQ_003499"
     CDS             1725..3461
                     /gene="argS"
                     /locus_tag="ACHURQ_003499"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR0825196.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(3503..3547)
                     /gene="yecV"
                     /locus_tag="ACHURQ_003500"
     CDS             complement(3503..3547)
                     /gene="yecV"
                     /locus_tag="ACHURQ_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR0825197.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3638..4126
                     /locus_tag="ACHURQ_003501"
     CDS             3638..4126
                     /locus_tag="ACHURQ_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR0825198.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4246..4638)
                     /gene="flhe"
                     /locus_tag="ACHURQ_003502"
     CDS             complement(4246..4638)
                     /gene="flhe"
                     /locus_tag="ACHURQ_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR0825199.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4638..6716)
                     /gene="flhA"
                     /locus_tag="ACHURQ_003503"
     CDS             complement(4638..6716)
                     /gene="flhA"
                     /locus_tag="ACHURQ_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR0825200.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6709..>7642)
                     /gene="flhB"
                     /locus_tag="ACHURQ_003504"
     CDS             complement(6709..>7642)
                     /gene="flhB"
                     /locus_tag="ACHURQ_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR0825201.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1946 a         2070 c         1869 g         1757 t
ORIGIN      
        1 taatcaatgt gaatataaaa aatattatta gcacaacgct atgttcactg tcacccgctc
       61 aacagatcac ggttatcgtt cgttttttat actgctcagg gataaaaaaa ggcccctgtt
      121 gaaattgcag gggcctggta cgagcaagca tcatattggg cgacatgatg caacggtaaa
      181 aatcatttgg cctgatggcg ttcaatgatt cctttcattt cagcaaccgc cgccccgtct
      241 acgatataac gcgaatactc gtccgcgtgt tcatctgatg acatggacaa tccttgatta
      301 cgataacgca tcggtgaggc ttgccacgct cccgcggagc tatggacttc cagcactccg
      361 gcatcgagga agtggtgcag gttttctgca cggactcctg ctccggccat aatgattgga
      421 gcatcacgat gggcaataag ttccataatt tttgataaac cttgcagtgc gtctgatttt
      481 tgccctgatg tcagtacccg ggcaatgccc aattccgcaa gattattgag tgtatataaa
      541 gggttagcgc acatatcgaa ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc
      601 attatttttt ccattcgtgg catatcgaca ttcccgtcaa catcgagaac gcccgtcacc
      661 agtccaggaa aacctaattc gcggaccgtg cgcacatcct caagaatggc ggcaaactca
      721 ccgtcgctgt agcaaaaatc accaccgcgt gggcgaatta tcggatgcac agggatcgtc
      781 acccgctggc gcacggattt cagtaccccc aacgacggcg ttaagccccc ctcttttggg
      841 gctgcgcata attcaactct gtctgcgccg ttttgctgcg ccgttagtgc acattccatg
      901 ctgtaacagc aaatttccag taatgccatt tttgctcctt aattacgctg actgctcgct
      961 ggcaacgatc tcttcaatgg accacggatg aaacttaata gttgttttgc catcggtgac
     1021 cgccagcgtc ggattcggta atcgctcatg ctcacctttt ggtgaactgg ttttaactga
     1081 gatccccggt aggctgagtc cttcatcaga aagcaatgcc agcgcacggg cattcagcgt
     1141 ttctggatca cccagcagaa cgatttcaat atgttcccaa ccttcgtgtg ggtaacgttt
     1201 ttccccgggc cacggtagct ccacaataga aaactgccag tgcgcaacct gtaccggttc
     1261 atgcaattta aacagacaaa tcggtctgcc attgatcata ttttctgaca aaagctcgcc
     1321 acactgttca aacccgcgac gccagcgttc agcagtggcg ttttgatggc aacgcaaaga
     1381 aatgtgatcg gcagtcagcg gagtgatatt caaccccaga cggagggaaa gttcatctaa
     1441 tgcgtgggta aatcgcggta aatccgatgc aatatcctgc agctcgtcga tagattgcca
     1501 gttcgccata atcactcttc gtctttcagt aaaagcgtta atttaccctg ttgccctgcg
     1561 ccaaccaacc gctgatttca cgccgcttct gatgcaatag tgaaaacggc aatacgccac
     1621 gcgcacgttg ctgacgaaaa cagccatttg cagtatactc ccgccctaat ttctttaact
     1681 ggtgcgggca atttttgctc gcttcatcaa tgtaaggtat tccggtgaat attcaggctc
     1741 ttctctcaga aaaagtccgt caggccatga ttgcggcagg cgcgcctgcg gattgcgaac
     1801 cgcaggttcg tcattcagca aaagttcagt tcggcgacta tcaggctaac ggcatgatgg
     1861 cagttgctaa aaaactgggt atgacaccgc gacaattagc agagcaggtg ctgactcatc
     1921 tggatcttaa cggtatcgcc agcaaagttg agatcgccgg tccaggcttt atcaacattt
     1981 tccttgatcc ggcattcctg gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg
     2041 gtgtttctac gccagaaaaa cagaccattg tggttgacta ctctgcgcca aacgtggcga
     2101 aagagatgca tgtcggtcac ctgcgctcta ccattattgg tgacgcagca gtgcgtactc
     2161 tggagttcct cggtcacaaa gtgattcgcg caaaccacgt cggcgactgg ggcactcagt
     2221 tcggtatgct gattgcatgg ctggaaaagc agcagcagga aaacgccggt gatgaaatgg
     2281 agctggctga ccttgaaggt ttctaccgcg atgcgaaaaa gcattacgac gaagatgaag
     2341 agtttgccga gcgtgcacgt aactacgtgg taaaactgca aagcggtgac gaatatttcc
     2401 gcgagatgtg gcgcaaactg gtcgacatca ccatgacgca gaaccagatc acctacgatc
     2461 gtctcaacgt gacgctgacc cgtgatgacg tgatgggcga aagcctctac aacccaatgc
     2521 tgccaggaat tgtggcggat ctcaaagcca aaggtctggc agtagaaagc gaaggggcga
     2581 ccgtcgtatt ccttgatgag tttaaaaaca aggaaggcga accgatgggc gtgatcattc
     2641 agaagaaaga tggcggctat ctctacacca ccactgatat cgcctgtgcg aaatatcgtt
     2701 atgaaacact gcatgccgat cgcgtgctgt attacatcga ctcccgtcag catcaacacc
     2761 tgatgcaggc atgggcgatc gtccgtaaag caggctatgt accggaatcc gtaccgctgg
     2821 aacaccacat gttcggcatg atgctgggta aagacggcaa accgttcaaa acccgcgcgg
     2881 gtggtacagt gaaactggcc gatctgctgg atgaagccct ggaacgtgca cgccgtctgg
     2941 tggcagaaaa gaacccggat atgccagctg acgagctgga aaaactggct aacgcggttg
     3001 gtattggtgc ggtgaaatat gcggatctct ccaaaaaccg caccacggac tacatcttcg
     3061 actgggacaa catgctggcg tttgagggta ataccgcgcc atacatgcag tatgcttaca
     3121 cgcgtgtatt gtccgtgttc cgtaaggcag aaattaacga agagcaactg gctgcagctc
     3181 cggtaatcat ccgtgaagat cgtgaagcgc aactggcagc tcgcctgctg cagtttgaag
     3241 aaaccctcac cgtggttgcc cgtgaaggca cgccgcatgt gatgtgtgct tacctgtacg
     3301 atctggctgg tctgttctct ggcttctacg agcactgccc gatcctcagc gcagaaaacg
     3361 aagaagtgcg taacagccgt ctgaaactgg cacaactgac agcgaagacg ctaaagctgg
     3421 gtctggatac gctgggtatt gagacagtag agcgtatgta atcgattttt cgtgagagta
     3481 aagcctgatc agggttacct gatcaggctt ttttattgcc atctaaatgt attctgaaaa
     3541 tggacatgcc attgttttct gactgttgga taagaggcca gaagcgtaat atccggcccc
     3601 agggaaacga taacggttga atttaaggaa taccgcagtg tttaaatttc ttgtattaac
     3661 attaggcatt atctcttgcc aggcttacgc agaagatacg gttatagaaa acgaccatga
     3721 catttcagcc atcaaagatt gttggcaaaa aaattcagat gatgatactg acattaacgt
     3781 gatcaaatca tgcctgcgac aagaatacaa tctcgtcgat gcgcaattaa ataaagccta
     3841 tggtgaagct tatcgttata tagaacaagt gccacgcaca ggtgtaaaaa aacctgatac
     3901 cgaacaactt aacttgctta aaaaatcaca gcgagcctgg ctggatttta gggacaaaga
     3961 atgtgaatta atcctttcaa atgaggacgt tcaggattta agtgaccctt attctgaatc
     4021 agaatggctc tcatgtatga tcatacagac caatacgcga actcgccagt tgcagctata
     4081 ccgtaactct gaagattttt atccaagccc tttgacaaga ggataattca catctttttg
     4141 gcatgttttg ttgcaagcta ttcctgataa ataattgcaa caagacatcg agcctttttc
     4201 actgagttat taaacatact cgcgagcgcg taattttttt gtccttcagc gataattcac
     4261 aatcacttca ttacgttgta cctttagcgg tggaattaac cgcccaccgc ctggcacttc
     4321 ccagataaat cgcaacggtt ctgctgccgc tataccggaa aaggccacgg tggttccgct
     4381 ctgcccctct aattcgacac aacgagattg cgaacacaag cgaacccgca gtcctgaagg
     4441 tgtcgggccg ataagctgat aacgccacgc taccagcgtc atcaatcctg aagcaggttg
     4501 tcgcgtagaa agaggcgcag acgacatcga ctcaccgcga tgatttagcg taattcctac
     4561 actacttgcc tgccacatcc cctccccggc ggcttgcacc agcagcggaa acaataatat
     4621 tgctaataag gttctcatta tttgccgcca attgtcgccg tcatgcggat atgtcggtta
     4681 tcagacagtt ccagattcga caggaccact aactgcggca agctgcggcg caggaagcga
     4741 gacaataatg gtcgcagcgc gtggttcacc aacaatactg gcggcgcgcc cagcatctcc
     4801 tgacgggata gcgcttcctg agtttgcgcc agtaaacgat ccgccagccc tggctccagt
     4861 cctcccccgc cctgcagcgc ctgtagtaac aaacgttcca gcggtgtatc gaggccaata
     4921 acatggactt catctttgcc aggaaaccac tgctgggtaa tcgcccgtcc caacgccacg
     4981 cgcacgacgg cggttaattc atgtggatcg ctttggatgg gcgcatgttc cgccagcgtt
     5041 tcgagaatgg tgcgcatatc gcgaatcggt actttttcat cgaggagatt ttgcagcact
     5101 ttatgcagtg tggtgagcgt gacgacgcca ggaacgagat cttccgtcag ctttggcatc
     5161 tcctgggcga cgcgatccaa cagctgttgc gcctcctgac gaccaaacag ctctgcggca
     5221 tgctggctaa tgaggtggtt aagatgcgtt gctaccaccg tgctggcctc aaccactgtg
     5281 tacccctgaa tctgcgcctg ttcttttagc gcactttcaa tccagatagc attcaggcca
     5341 aatgccggat cgacggtcgc ctcaccaggt aacgtcccgg cagcggttcc agggttaatc
     5401 gccagccagc gccccggata agcatcacca ctgccaatct ccacgccttt catcaaaatg
     5461 cgatagcggg caggttgcag atccatattg tcgcgaatgt gcaccactgg cggcagaaat
     5521 cccatctcct gggcaaattt cttgcggata ctgcgtatac ggcccaacaa ctcaccatcc
     5581 tgctggaaat cgaccatcgg gatcagtcga taacccactt ccattcccag agaatcttcc
     5641 agttgtacat cgttccacgt cgcttcgaca acggtattat tctctgccat ttttaccggt
     5701 ttgggttcgg caggcgcttt ttgttcgcgt ccgcgtatcc accaggccag cccgagcaat
     5761 ccggcagtga acagcaaaaa taccaggttc ggcattccag gcaccaggcc gagtaaaccg
     5821 agcacggcgg cgcttaacaa cataacgctt gggttactga aaagctgatt caccatctgc
     5881 tcgccaacat cctgatcggt gctgacacgc gtaacgatga cccccgcggc ggtagaaatc
     5941 accagcgccg gaatttgtgc caccagaccg tcgccaatgg tcaatagcgt ataactttcc
     6001 gccgcgtgtc ccatgctcat gccatgttgc agcacgccga ccagcaaccc gccgacaacg
     6061 ttaatgacca tgatgaggat cccggcgatg gcatcgccgc gaacaaactt acttgccccg
     6121 tccattgagc cgtaaaaatc ggcttcctga gtcacttcgg agcggcgttt tttcgcctca
     6181 tcttcaccaa tcaatccagc gttaaggtcg gcgtcaatcg ccatctgctt acccggcata
     6241 ccatcgagaa caaagcgcgc acccacttct gcgatacgcc cggcaccttt ggtaatgacc
     6301 ataaagttga tgatcacgag aatgacaaac accacgatac cgatagcgaa attgccacca
     6361 acgaggaagt gaccgaacgc ttcgaccacc ttccctgccg ccgccgcgcc ggtatgccct
     6421 tccatcaaaa tgatacgggt tgaagccacg ttaagtgcca gacgcaacaa cgtagtaaac
     6481 aacagaatgg tcggaaacgc agcaaactca agcgtgcgct gggtaaacat cgccaccagc
     6541 aacaccatga tcgacaaggc aatattgaag gtaaacaaca ggtcgagtat gaatgcgggc
     6601 agtggcagca ccatcatcga caagatcaac aggatcaaaa tcggtccggc aaggatctgc
     6661 cattgtgtcg atttcaggtt tgcgggcagg cgcagcatcg cggccagatt actcatgggt
     6721 cggtttctcg ttaataaaat ccagggcttc cggcaccgga agatgagtag gttgtacagg
     6781 gcgctgtcca ccagccagac gccagcgttt cagttgccag acccaggcca gcacttccgc
     6841 caccgcggcg tacagttgac ccgggatttg ttgaccaatc tccgcatgtc gatacagcgc
     6901 tcgcgccagc ggcggcgctt caagcgtcgg gacgttattt tcagcgccaa tttcacgaat
     6961 gcgcagcgcg accagccctg cacctttagc gaccactttc ggtgcgctca ttttgttttc
     7021 gtcatactgc aacgctaccg aatagtgggt cgggttattg acaatgacat cagctttcgg
     7081 cacatcggcc atcatccgac gccgtgcagc agctcgctgc atctgacgga tccgcccttt
     7141 aacatgaggg tcaccttcgc tttgtttgaa ctcatcacga atatcctgcc gcgacatacg
     7201 cagctttttc aggtggctga agatttggaa aaagacgtca aatcccacca ttggaatgac
     7261 accaagcacc accagcagtg cgcatagccc aaccagatcc atcgcattac ccatggcggt
     7321 agtcggagac tcggccatta agcgcatcat ctgcggccag tgatgccaga gataaaaccc
     7381 cgtcacgctg ccaaccagga tggttttcaa aattgcctta agcaactccg cgccagtctg
     7441 tgccgagaac atccgtttaa tgcccggtag cgggttgagt ttggaaaact tcggctgcaa
     7501 ggatttgccg ctaaatacca gcccccccag catgaccgga gaaatgagcg ccaccagcac
     7561 cacgccgcta atcagcggca gcagcgccag catggcttct ctgatcagca gaataatctg
     7621 cccgaggatc agattcgggt ca
//
LOCUS       DAZOZY010000191         7632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZOZY010000191 DAZOZY010000000
VERSION     DAZOZY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.192"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            399..1298
                     /gene="yegS"
                     /locus_tag="ACHURQ_003505"
     CDS             399..1298
                     /gene="yegS"
                     /locus_tag="ACHURQ_003505"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR0825202.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            complement(1380..2153)
                     /locus_tag="ACHURQ_003506"
     CDS             complement(1380..2153)
                     /locus_tag="ACHURQ_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0825203.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2259..3299)
                     /gene="gatD"
                     /locus_tag="ACHURQ_003507"
     CDS             complement(2259..3299)
                     /gene="gatD"
                     /locus_tag="ACHURQ_003507"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0825204.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3347..4702)
                     /locus_tag="ACHURQ_003508"
     CDS             complement(3347..4702)
                     /locus_tag="ACHURQ_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR0825205.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            complement(4706..4990)
                     /gene="gatB"
                     /locus_tag="ACHURQ_003509"
     CDS             complement(4706..4990)
                     /gene="gatB"
                     /locus_tag="ACHURQ_003509"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR0825206.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(5021..5473)
                     /gene="gatA"
                     /locus_tag="ACHURQ_003510"
     CDS             complement(5021..5473)
                     /gene="gatA"
                     /locus_tag="ACHURQ_003510"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR0825207.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5483..6740)
                     /gene="gatZ"
                     /locus_tag="ACHURQ_003511"
                     /pseudo
     CDS             complement(5483..6740)
                     /gene="gatZ"
                     /locus_tag="ACHURQ_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            complement(6769..7623)
                     /gene="gatY"
                     /locus_tag="ACHURQ_003512"
     CDS             complement(6769..7623)
                     /gene="gatY"
                     /locus_tag="ACHURQ_003512"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR0825208.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
BASE COUNT         2024 a         1969 c         1701 g         1938 t
ORIGIN      
        1 tcccccatta aataccaacg acactctcta gtgtttaaat ataataatgg catgattatt
       61 ataattgaat aggattataa taaatgttct gtacaacatt tcctacataa gtaggaatta
      121 cggacattga ggcccttcag ggtaactcca tgggggcttt aatatattat attgaagatg
      181 ccactgttta gttgaatatt aggtatatgc tcttttttga aatttatcgg tggcagccgt
      241 tagtattcgc tgtccccatt gcaagctcct ggtggtaacc actgaatcct ccatacttga
      301 actgactttt tatcctccga ctttcatcct gttctgactc caccttttgt tttctgctct
      361 acactatcta cagaccaatc ataaaggcac atacgatcat ggcagaattt cccgccagct
      421 tactgattct taatggcaaa agtactgaca atctaccctt gcgcgaagca attatgctgt
      481 tgcgtgagga aggaatgacg atccatgtgc gggtcacctg ggagaaaggc gatgccgcac
      541 gatatgtaga ggaggcccgg aagttgggcg tcgcaacggt gattgccggt ggtggcgatg
      601 gcaccattaa tgaagtttct acggcgttga ttcagtgtga gggggatggc atacccgcgc
      661 tgggaatttt gccattagga accgccaatg attttgccac cagtgtaggg attcctgagg
      721 cactggataa ggcgctgaaa ctggcaattg ccggtaacgc cattgcgata gatatggcgc
      781 aggtcaacaa acaaacctgt tttattaata tggcgacagg cggatttggg acgcgtatta
      841 ccacagaaac gccggaaaaa ttaaaagccg cgctgggtgg cgtctcttac atcattcatg
      901 gcttaatgcg catggatact ctgcaaccgg accgttgtga aatccgcggt gaaaactttc
      961 actggcaagg tgacgccctg gtcattggta ttggtaacgg gcgtcaggcc ggtggcggtc
     1021 agcaattgtg tccgaacgcg ttaattaacg atggcttgct gcaactgcgc atttttaccg
     1081 gcgatgaaat tcttccggct ctcgtatcaa ccttaaaatc tgacgaagat aacccgaata
     1141 ttatcgaagg cgcttcgtcg tggtttgata tacaagcccc acacgaaatc acttttaatc
     1201 ttgatggcga accgttgagc ggacaaaact ttcatattga aatacttccg gcggcgttgc
     1261 gttgtcgatt accaccagat tgtctattat tgcgttaatc aaactattct ctcttgaacc
     1321 agggagaatc cgaaatgccg gatgcggtgt aaatacctta tccggcatac agaacaatac
     1381 tacaccgtaa ttaattttac ccgtgtcttc tgcaatgcca gtttatccac ctctgataca
     1441 gcatcatcgg taataataat gtcaattttt tcgattggca gtacctgatt aaaaccgcga
     1501 cggttaaatt tcgatgaatc gagaacggca atcactttat tagcggcggt gaccatcgca
     1561 ccgctaatcg aataaccttc attaaaggtc gtaataccat taaccgcatc aataccatca
     1621 gcaccgacaa acattaaatc ggcattaata tcctgcaatg aacgctcggc aatggaacca
     1681 tgcatcgagc gcgttttatg gcggaccgtg ccaccacaga cgaccagagt aatgtcttta
     1741 ttttcggaaa gggcaaacgc tgccgggaga ctgttggtaa tcaccgtgat atttttagcg
     1801 gtcattaatc cttcggcgat aagcattgtc gtacttccgc tgtcgagaat aacggtcatc
     1861 ccttcatgga tcattttgac tgccgcctgc gctattctgt tttttggcgc gctggcgagc
     1921 tgaaatcgct ctttaaaccc gacttcctgg gtctcggttt cactattacc ggacattatt
     1981 ttcgccgcac cgccatgaaa gcgcgtaacc acgccttttt gttcgagaaa gcgcaaatcg
     2041 gcacggattg tcgcttccga ggcagcaaat actcccgcca gatcctgaac aagcacggtt
     2101 ccctgttcat tcactaattg gatgatctta ttccttcgct cgaatgaatt catcgtcatt
     2161 tacccatatt caattgtggc tagtgtaaac gaagggggac cattggtgaa tgatctgttt
     2221 tgcacaatac cgggccagca tcacgctggc ccgcggtttc atggaatgag caacactttg
     2281 cccggcatgg cattacgagc gatgtcacgc accgcctggg tgaagctttc aaagcttcca
     2341 cggtgagcga ttaatggctc caggcttaac ttacgttctg tcagcaaccg gctcgccgtt
     2401 tcccactcct gccccggcca agggctggaa tagttcatcc aactgccgat aaccgtcagc
     2461 tctttacgca atattttgcc aaacgttgcc gatgttaaat gcagatcctg atgcaacgtg
     2521 cccaccagcg ccagttgggc atgaggaccg gcaatctcta ccgccagttc gacagtttgc
     2581 ggtacgccag ccgtctcgag gataagctga ttaaagcgca gttcgcgtaa aacgctctgc
     2641 atttgcggcg cgctcatttc gctactgtta aatgtttgca tcgcaccgaa agattttgcc
     2701 aatgccagtt tttctgagct aatgtcgatc gccgtcacac tctttgctcc cagcgcgaca
     2761 gcgcactgaa tagccagcag gccaatggtt ccggcaccaa taataataac gtttttattc
     2821 tcacaacctt gcgctaaatg aaaagcatgc aggccaacgg taatcggctc aataaaagcc
     2881 ccatcctcaa taggcatatc cgtgggtaga gcaaagacat tttttcgctt aacgacaata
     2941 tattcagcaa atccaccatc acgccgcgag ccaataaaat catattttgc gcactgggaa
     3001 taaaaccctt tcaaacactc tggataagta aaacagggta ataacggcac acaggcaacc
     3061 gcatcgccag ggtgtaaatc atcaacaccg gatcccaccg catcaatata gccgctaaat
     3121 tcatggccta acgttattgg ataataatgt gcaccatttt taaatatcct gggtaaatcg
     3181 gaaccacata agcccgagct ggcaattttt acccgcacct catcctgatg tttaatttca
     3241 ggaatgacgc tttctgcaac gcgcacgata ccatcagtat cattcaccat tgatttcata
     3301 aaaactcctg attgttaagg gggataacct ccccctcagg taaaaattat tctgcgagaa
     3361 cggttttctc ttgtttaata aaaccacgcg ctctacgcca ggtcatgaaa ataccggtaa
     3421 gataaattgc gccgataata atgaaaccgg gaatattttg cgtggagaaa acctgaatca
     3481 gtaaccaggt aatgggagaa ccgccctgat ccattgaagc caccataccc ccggctttta
     3541 acgccccagc attagccgcc agttgggtgt gcaaaccaat agtttgcgtc gcgatccaca
     3601 gggtgatgct cataatgatg acacccgaga ttaaggtgcg gaacagattt ccacgatgca
     3661 ccgcgaccgc catcgccacg aagaagccga tggtggcaag atcgccaaac ggcagtacct
     3721 gattacccgg aacacaaaca gcaattaaaa tggtgagcgg gataaaaatc aggcttgccg
     3781 ataccaccgc cgtatgcccc agcagtaatg cgggatccag gccaatcagg aactcctgac
     3841 cgccgaactt cgcctgtaaa cgactacggg cctgcttagc gatgggcgtt aaaccatcca
     3901 tgatgggttt aatcacccgt ggcatcagca gcataactgc cgccgttttt accgccagct
     3961 gcaatacacc tttgacatcg taacccgcga ggatgccgat aatcagcccc atcacaaaac
     4021 ccacggtgac aggctcgcca aatggaccaa atttgcgctg aatatcgtcg gcgctaaatt
     4081 taattcggtt aacgcctggg attttctcga tgatagcatc gaccagcacc gcaatcggcc
     4141 ccatatacgc cgacgtaccg tgcggaatag caataccttc cagctcaaag aaatttcggg
     4201 tatcgcgggc aaaccagtcg ccgagcttat aaacaaacgc cgcgtgaatt accacacctg
     4261 ccatccctat catccatgaa ccggttgcca gatgcagcaa cgcgccggtg aaggtcatat
     4321 gccagatatt ccagatatca acatttacca cccgcgtcat acgggtcagt aacatcgcca
     4381 cgttaaccag aatcgcaatc ggaatcgcca ccagcgcaat ttgcgacgcc caggtcattg
     4441 gtgaagagcc cggccagccg acatcgacca catgcagatt caggtcgaaa ttttccgcca
     4501 tcgctttcgc cgccggacca atggaatcca gcattaagcc aatcacaagg ccaatgccaa
     4561 caaagccaat cccgatatgc agacccgctt taaagcaatc gcctgccttc atgcctaata
     4621 ttttagaaaa aataatgatg acaatcggaa gcatcaccgt agggccgagg tcgagaatat
     4681 aacgcatgac ttctgaaaac ataggtcacc cctgtaagat agtcagaatt ttattttgta
     4741 atgcttcgat accgacacca gaaataaaag gcatgccgtg aactaacgga atatcgccaa
     4801 aactacgatc cactttggca gtggtgcata tcaaatgcac accatccata taggtttcta
     4861 tttcattaac ccgacactgg attaattcaa caggaatact atgattctga cacaactctt
     4921 taatttcttc cgccgccatc gtagaggtcg caaccgcgcc tccgcaagcg acaataatct
     4981 tgcgtttcat aaatagtcct ttttattgtt acagagggat ttattcgtct gaatctaaaa
     5041 catactttgt gaagtattcc tttaactggg tttcaggaag agtgattagt gtctcgacga
     5101 tatcgggctg ttgtaacttg ccaaataaac agcgtaaaag tttcaattgc tgctgcggat
     5161 tctccacaat caacgcaata accaacgata cctccacgtc gttatcatca tccgcttgct
     5221 gaaaatgaac tttatttgtt ggccttaaca gataaatggc tgacgactta gcatgaatcg
     5281 cctcacaatg cggtattgca atggcgtgct gctcaagcat tatcccggta gggaattctg
     5341 cttctctggc aattaatgcc tgtggccagg tatcatgaac cacacctttg gcgagcatct
     5401 cattaccgat atgggttaaa acttcgctac gatcgacaaa agaaattccg ctacgaacaa
     5461 acaggttagt catataccgt ccttattccg cacagccgta gcgataggcg cgcaaaacat
     5521 cataaatttt atccataatc agctgatgcg gtattgctga taattccccg gactgaatgc
     5581 gttcaaattg tttgggaaga tactgactaa tcatgcccag tgggatgtcc acgccttcaa
     5641 gattcaccat catcgtttcg acgctatttt taatccgact atgcggccag taataacgaa
     5701 tacgatccga caggctgtaa cgaatatcca gtaatgaatc gttaaaaccc gtacgataat
     5761 attttttcca gtattgcggt tcgtcgagca tcacttcttc aattaccgcc aggcaaccgc
     5821 tgcgattttc aggggcgata agttcctgct caatttgtgc cagtgcaaat atcgcctcgc
     5881 gtaaagcaaa ggttaatgcg ggaccgactt tcaatattgc aaagtgatcg cggactaatt
     5941 cccaataagc cgtccgggtc tggtaatcgg tagaatgtgc ttcataaacc attcgggtgt
     6001 tttctatcca ttgcgccagc ggctgcgctt cctgcggctg ataatggata atattgctgt
     6061 gatcaaattc cacgcccggc tgcaccacga tggcaatcac gcgtgttaac gcctctgtca
     6121 gcccacgggc aataaaggcc ttttgatgcg tacgtaaagt attggcggca tcttcaacat
     6181 gggtgatgtg tactgactga atggcgctgg cctcaccgcc cggaaccgga acttcagtgc
     6241 caatgacata gctcagttgc tcacgctggc aatctgtcgc cacactttcc gcagcaaagc
     6301 aaagcagctc gttccgcaac cgtttctggt gctaacggta tgggatcccc cgcgcaggac
     6361 attgacgcat caagatgaat tttactgaag ccagcacgaa catatgcttt taccagctcg
     6421 acggattttt ccatcgccgc atccgcattt tcttgctgcc agcagtttgg ccccagatga
     6481 tcgccgccga gaataatgcg ttcgcgtgca aatccgactt tatcggcaat cgtaaaaaca
     6541 aattcgcgaa agtctgccgg tgtcattccg gtataaccgc caaattgatt gacctggttt
     6601 gacgttgctt caatcagcac tttgcgcgtg ctgttgcgat caaatgccag cgccgcttcg
     6661 ataaccaacg gatgggcaga acagactgaa catatgccga tatgttcacc agctttatgc
     6721 cgggcaatta acgttttcat gttttttcct tgttaaatgg caggtgcgtt atgccctgcc
     6781 ctcgcagcca caatcggcaa tcactttgct caccacatcg cgcattgcgg atttagccga
     6841 ctgcaaataa tcccggggat cggtcgcttc agggtgctcg gtcaggtaat tttttaacgc
     6901 ctgcgagaag gcatttttca gctccgttgc aacgttgatt ttgcatatcc ccagtttgat
     6961 ggtttgctga atatccttag tcgataaccc tgacgcgcca tgcagcacca gcggtaagtt
     7021 cacccactgg cgaatgttct ccagtcgaga aaaatcaagc gccggtgcgc tggcatacat
     7081 cccatgagcc gtgccgatgg cgaccgccag ggaatcaatt ccggttgcct cggcaaattc
     7141 acgcgcctga gcggggttgg tataaaaaac atcggcttca ttgacttgca catcatcttc
     7201 ctggccgcca agttgcccca gctccgcttc gacgctgaca tcaaagcgat ggcaaaaatc
     7261 caccacctct ttgacccgtg aaatattttg cgcaaaaggc aaatgcgagg cgtcaatcat
     7321 gactgagcgc acgccagaac gaaccttctg agcgatatcg tcaaatttcg tgtgatggtc
     7381 gagatgaatt gccagtggat ggtgatattg cttcgccatc gcgctgacca gcgccaacag
     7441 attttctgta ccagcataag tgaatgtgcc aggcgttccg gcgatgatga ccggcgcatg
     7501 caggttggca gcggtttcca ccaccacttg catcgtttcg agattgtgaa tattgaatgc
     7561 cggaaccgca taaccgccgc gctgtgcgtt gttcagcatc tgctttgtcg ataccacgta
     7621 cattttcata tc
//
LOCUS       DAZOZY010000192         7611 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZOZY010000192 DAZOZY010000000
VERSION     DAZOZY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.193"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            171..701
                     /locus_tag="ACHURQ_003513"
     CDS             171..701
                     /locus_tag="ACHURQ_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR0825209.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            837..1502
                     /locus_tag="ACHURQ_003514"
     CDS             837..1502
                     /locus_tag="ACHURQ_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825210.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1824..2447
                     /locus_tag="ACHURQ_003515"
     CDS             1824..2447
                     /locus_tag="ACHURQ_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR0825211.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2532..2750
                     /locus_tag="ACHURQ_003516"
     CDS             2532..2750
                     /locus_tag="ACHURQ_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0825212.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2728..3798
                     /locus_tag="ACHURQ_003517"
     CDS             2728..3798
                     /locus_tag="ACHURQ_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0825213.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3933..5216
                     /locus_tag="ACHURQ_003518"
     CDS             3933..5216
                     /locus_tag="ACHURQ_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR0825214.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5450..>7611)
                     /locus_tag="ACHURQ_003519"
     CDS             complement(5450..>7611)
                     /locus_tag="ACHURQ_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR0825215.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1896 g         1759 t
ORIGIN      
        1 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcatagca gaactggagg
       61 cacagacagt taccgttaaa gaggttggag atgcttgata gttttgcgct cgttgcactg
      121 gttggcattg tagcgttgat ttgggtgaca tccgggaatc ggaggcataa gtgaacgaca
      181 aagaactgat taaagaaatc aaagagcgta tcagcagttt ggatgtgcga gacaatgttg
      241 aacgccgtgc ttatgaaatt gctctggcat cgctgacagc agagccggtg gcgtggaagg
      301 taaccttcac gcaaattgac cgtgaatata acacgttcac tggtatgtat tctgacaaag
      361 cagaagtcga acggtgggtg cggctgcata aagcatgtaa ttttcgggca gatataacac
      421 cgctttatac cgcccagcca gtgccggtaa ctccggatgc ctggataagc tgtagtgagc
      481 gaatgccaga aatgggagag cgacaatgct atgtgttagc agctgacttt aaaaacaact
      541 acccaccaaa catccccaac actcaggtcg gcgtatatgg cgactggttt aatgatggca
      601 atcccacttg ggatgacggt gatggcgaag acctgtatct caaagaggta acccactgga
      661 tgcagctacc agaaccgccg caggaggtta accgtggcta acctgcaact tgccgtcaaa
      721 ggtgaataac aatcctcgca ctcgcgggga tttcttttat acgatatagg ggaaaaggtg
      781 tggttaacaa tattgcaaat cactcttcgt ttccggctgt aacaatcgac agccagatgc
      841 tgttgaaaat ggtcaatgaa gctcgcaagt tatatggaga gccatcagta cgtaacaaca
      901 aattcatcga gaaaattgag gatgaattgg aaggcgagac ctacacaaaa agtgtaggtc
      961 ggaaaaacgg ggctgacatt gatgttatct ccatgactat caagcaggcg cttcgtgttg
     1021 ctgctcgcga atctaaagca gttcgccgaa cacttgtaga caaacttgaa agtatgcagg
     1081 aagcgcacat taaaagcggt aaatcagcga gtggacttgt tgagtatcgt caagcgcgaa
     1141 cactgaaaat gacggttgaa gctgttacca atctgttcga tttgatgcca aatcttgcgc
     1201 cggaagcaaa gcagactgct gcggcaagca taatcaaccc gctcgttggt tttaatgcaa
     1261 tacctcttcc ggcaatagaa gagcattact actcagcagg ggaggtcgca gagcagctcg
     1321 gagtaacggc aaacaagatt ggtcgcattg ctaacgcaaa caacctcaaa actgagcagt
     1381 acgggaagtt cttcctggat aaatctgcgc attccagcaa gcaggttgaa acatttcggt
     1441 acaacgaggc tggagtgcac aaaatagagg aactcatcga aggagagcga aaggctgcat
     1501 gattttgaca agatagtttt ccccaaatgt ggggaaaagc ccgaatggcg cggcttacag
     1561 caagataagg cctacatgat ttgacaacac cgcattaacg gggctatatt ccgcttcatg
     1621 gtgctgaaca caccttgcaa agcggaaacc gcacccgtca gtcatgcgga ttttttatgt
     1681 ccatttttca gatatggtcg ggtagcgcgt ataccgaaaa acagccgaaa ggttaaggat
     1741 acgggccgac tttgcacggt gttcaagtac ctgaccgccc tgctgaacac agggctatct
     1801 gaacaaatgc aaaggacata aaaatggcca gtcaactcat ccccgtattc aacggcacta
     1861 tatccaacga aacaactctt ctcgttaatg cccgtgattt acacgaattt ttggaagtac
     1921 gtcgtgactt ctctacatgg attaaaaatc gcataacaga atacggcttt gccgttgatg
     1981 tcgattacat tttggttcac caaaacaggg ggatcaaagg acgtggaggc gatcgccgta
     2041 gcaaagacta tcacctcacc ctcgatacag ccaaagaaac ggcgatggtc gagcgtaacg
     2101 aaaaaggccg ccagatacgc cgatacttca tcgagtgcga aaagaaactt cgcagcatgc
     2161 aaccagcgca gcaattcaca gacgaagaaa tcatcctcct ttgctatatg caggtacaga
     2221 tggagaatgc gcaggacatc tgcaaacgtc tgtacccgat attgaaggaa ctgaactcat
     2281 catacacgag caagctgtat gacatcgcgt ttgaaacttt ttacacggtg acgaaaaaca
     2341 gagatgtact gctaagggag gcgacacgac ttgaccaagc aagcgccctt ttcgaacggg
     2401 caaaaccaat gctgaaaagc cttcgggcga gacaattcga attttaatca tcaaaggagc
     2461 ttcggctcct tttttgttgg agaaaataaa ccaatactcg ctcccttgcg agtaattgcg
     2521 gagactttgc gatgtacttg acacttcagg agtggaacgc acgccagcga cgcccaagga
     2581 gccttgaaac agttcgtcga tgggtacgcg agtgcaggat attccctcct ccggttaagg
     2641 atggaagaga gtatttgttc cacgaatcag cggtaaaggt tgacttaaat cgaccagtaa
     2701 caggtagcct tttgaagagg atcagaaatg ggaagaaggc gaagtcatga gcgccgggat
     2761 ttacccccta acctttatat aagaaacaat ggatattact gctacaggga cccaaggacg
     2821 ggtaaagagt ttggattagg ccgagacagg cgaatcgcaa tcactgaagc tatacaggcc
     2881 aacattgagt tattttcagg acacaaacac aagcctctga cagcgagaat caacagtgat
     2941 aattctgtta cgttacattc atggcttgat cgctacgaaa aaatcctcgc cagcagagga
     3001 atcaagcaga agacactcat aaattacatg agcaaaatta aagcaataag gagggggctg
     3061 cctgatgctc cacttgaaga catcaccaca aaagaaattg cggcaatgct caatggatac
     3121 atagacgagg gcaaggcggc atcagccaag ttaatcagat caacactgag cgatgcattc
     3181 cgagaggcaa tagctgaagg ccatataaca acaaaaccgg tcgctgccac tcgcgcagca
     3241 aaatcagagg taaggagatc aagacttacg gctgacgaat acctgaaaat ttatcaaaca
     3301 gcagaatcat caccatgttg gctcagactt gcaatggaac tggctgttgt taccgggcaa
     3361 cgagttggtg atttatgcga aatgaagtgg tctgatatcg tagatggata tctttatgtc
     3421 gagcaaagca aaacaggcgt aaaaattgcc atccctacaa cattgcatgt tgatgctctc
     3481 gggatatcaa tgaaggaaac acttgataaa tgcaaagaga ttcttggcgg agaaaccata
     3541 attgcatcta ctcgtcgtga accgctttca tccggcacag tatcaaggta ttttatgcgc
     3601 gcacgaaaag catcaggtct ttccttcgaa ggggatccac ctacctttca cgagttgcgc
     3661 agtttgtctg caagactcta tgagaagcag ataagcgata agtttgctca acatcttctc
     3721 gggcacaagt cggacaccat ggcatcacag tatcgtgatg acagaggcag ggagtgggac
     3781 aaaattgaaa tcaaataatg attttatttt gactgataat gacctgttcg ttgcaacaaa
     3841 ttgataagcg atgctttttt ataatgccaa cttagtataa aaaagcaggc ttcaacggat
     3901 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
     3961 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaaccccg cccgatcaac
     4021 gtccttctga tcaaaccgcg cctggtacct cttctcgccc gattctgtcg gcaaaagaag
     4081 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct gcagcgtgga
     4141 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggcactc
     4201 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
     4261 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggta tatgtccctg
     4321 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
     4381 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
     4441 gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag agcaaacgca
     4501 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
     4561 tgcaaaatct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt aaccatccgg
     4621 cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac attctcggtc
     4681 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
     4741 agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc gtttctggtc
     4801 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt actcagcaag
     4861 aagcgtatgt gtttgcaccg gctacgctgt ccaacatgta ctacggtttc ctcgccgtaa
     4921 acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg ctggatgttg
     4981 atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa ggttttaaca
     5041 cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt aataccggca
     5101 gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac aaccgcatgt
     5161 gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag aagtaagagc
     5221 aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
     5281 tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgga tgctgcgtaa
     5341 atgccttatc tggcctacag attcgatgcg attcgtaggt cggataagat gcgcaagcat
     5401 cgcatccgac aataagtgcc gaatgcgacc tacattcaca tggcgctttt tacatctgac
     5461 ggtttttatt gaagttaatc aaactacccg ccttgataat ctcgcgctct tcagcagtca
     5521 gactttccat atagagcgta atttccgtta ccggcgcatc ttcatggatc acataacctt
     5581 taaacgtcgt acccggatta tccagcgccg ctttaatgcc agggatgtaa atgtaatccc
     5641 ccacttcaaa ggttggcact tccgccatct gcagaggtaa catcccccag ttgatgacgt
     5701 tagaacggta gcgtttagtc gcgtactcct cggcaatatt cgccagaccg ccaatcacac
     5761 gctggcagct cgccgcctgt tcacgcgcag aaccatcgcc tggtttcacc gcatagacca
     5821 tgctgccaat ttcagtttgc agcggatcaa tatgctcctg accagcaatc tgcttaatgc
     5881 gcgcaaacac ctctgtcagc tcgctgacat tccccgccag acgctgattt tccagctcag
     5941 cagtcgcttt acttctgcca acataaccgg gatcacggcg agacagggta aactccgcca
     6001 gaccaatcgg atttgaacga taagaagagg tttcaccgga aggaatcagt tcgtcggtgg
     6061 tggtcacttc gtcgaggatc ttcgagcaca ctttcaggac gatattgtca gtcagcgcac
     6121 ccaattccgg ccagtcttta atgttcggcc cgtaaatcag cggttgctga gttgcccctt
     6181 tcacaaagcc ctgataaaca cggtttttat acggcgttac atcgaaggcg tactccggca
     6241 cgttgtccca gcaatcaagt tcgctggcag aggttaaata gccaccgttt gccgcagtcg
     6301 cagcgataga acgagcgtcc atcaacgcca ccgctgacat ctgcccatta gctggcttag
     6361 agccttcgcg gttcgggaag ttacgcgtgg tgtggcgaat actcaaaccg ttgttgattg
     6421 gcgtatcgcc cgcgccaaag catgggccgc agaacgcggt tctgatgatt gcgcctgcgc
     6481 caatcaaatc tgctaccaca ccttttttgg cgagatccat aaacaccggc tgtgatgacg
     6541 ggtaaactgc cagcgagaag gtgtcattgc cacaggattg accgcgcagt gcattcgccg
     6601 ccgcgatgac gttttcgtag ttaccgccag aacagcccgc gataatcccc tgctgcactt
     6661 tcaggcgacc attttccact ttatccagca gcgagagttt ggctttaccg tgcgccacgc
     6721 gttcggactc aatttcaatc tcacgcagaa tgtcggtcag gttctggttc agtgtgtcga
     6781 tttcatacac gttgctcggg tggaacggca gcgcaatcat tggtttgatg gcgcttaaat
     6841 caacgctgat gcagccatcg tagtacgcca tcggttgagg gttaagctgg cagtaatcct
     6901 ggccgcgacc gtgcagcgcc agccagttat gcacttcttc atcagtttgc cagacggagc
     6961 ttaaacaggt ggtttccgtg gtcatcacgt caacgctgtt acggaaatcg gtagagagcg
     7021 cgctaacgcc cggtccaacg aactccatga ctttgttttt gacgtaaccg tttttgaaca
     7081 ccgcgccaat gatagccagc gccacatcct gtggccccac atacggcgca ggttttccgg
     7141 tcagatgcac cgcaaccacg cccggatagt cgatatccca ggtgtcatta agcagctgtt
     7201 ttaccaactc accgccaccc tcaccgactg ccattgtccc taatgcaccg taacgggtgt
     7261 ggctgtctga cccgaggatc attttgcccc cgcctgccat catctcacgc atatattgat
     7321 ggatgaccgc aatatgcgga ggcacaaaaa taccgccata acgctgagct gccgataaac
     7381 caaaaacatg gtcatcaccg ttaatggtgc cgccgacggc gcagagtgag ttatggcagt
     7441 tggtcagcac atacggcagc gggaaacgtt ccattccgga cgctttagcg gtctgtacaa
     7501 taccaacaaa ggtaatatcg tgagaggcta atgaatcaaa cttaatttta agtttatcca
     7561 tatttccgga cgtattatga gaagagagaa tagaccaggc aatagtgcct t
//
LOCUS       DAZOZY010000193         7491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZOZY010000193 DAZOZY010000000
VERSION     DAZOZY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.194"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHURQ_003520"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHURQ_003520"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR0825216.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHURQ_003521"
     tRNA            647..722
                     /locus_tag="ACHURQ_003521"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHURQ_003522"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHURQ_003522"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR0825217.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHURQ_003523"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHURQ_003523"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR0825218.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHURQ_003524"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHURQ_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR0825219.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHURQ_003525"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHURQ_003525"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR0825220.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHURQ_003526"
     CDS             complement(5917..6249)
                     /locus_tag="ACHURQ_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR0825221.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHURQ_003527"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHURQ_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR0825222.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1823 a         2031 c         1869 g         1768 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZOZY010000194         7424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZOZY010000194 DAZOZY010000000
VERSION     DAZOZY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.195"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHURQ_003528"
     CDS             complement(83..1591)
                     /gene="putP"
                     /locus_tag="ACHURQ_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR0825223.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            2013..5975
                     /gene="putA"
                     /locus_tag="ACHURQ_003529"
     CDS             2013..5975
                     /gene="putA"
                     /locus_tag="ACHURQ_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR0825224.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHURQ_003530"
     CDS             complement(6015..6653)
                     /gene="rutR"
                     /locus_tag="ACHURQ_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR0825225.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            6941..>7424
                     /locus_tag="ACHURQ_003531"
     CDS             6941..>7424
                     /locus_tag="ACHURQ_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0825226.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         1775 a         2057 c         1942 g         1650 t
ORIGIN      
        1 ccattcaatt tatatggcga ggcacattaa gggagcgcaa cccgctgctg aagccaaaca
       61 aaaccgccgc aggctaagtc ccttagtttt cctgcaaccg tgacggcgga gccgaatgat
      121 agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc tgacggcgct ttacccagca
      181 aactaaacac tacaatcccg atactgccga agatgaagcc cggaataatt tcgtacagtc
      241 ccagccagcc gaactgcttc cagacgataa ctgtcagcgc accgatgatc atccccgcca
      301 gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa cagcaccacc ggaccaaacg
      361 ccgcgccaaa gcctgcccac gcgtagctca ctaagcccag cacgcggttt tccgggtttg
      421 ccgccagcgc aatcgccacc agcgccacca ccagcaccat cacacgtcct acccacacca
      481 gctctttctg gctggcctgt ttacgcagaa acgctttgta taaatcttcg gtaatcgcac
      541 tggagcacac cagcagctgg caacttaagg ttgacattac cgccgccaga atcgccgaca
      601 gcagaatccc ggcaatccac gggttaaaca gaatttgcgc cagttcgata aacacgcgtt
      661 cagcgttctg gtttaccgca cctgctaccg ccggatgctc gttaaagtaa gcgataccaa
      721 agaagccaac agccaccgcc cctgccaggc aaaggatcat ccaggtcata ctgatacgac
      781 gcgcatggac aatgctgtgg tgagaatctg ccgccataaa acgcgccagg atgtgcggct
      841 gcccgaagta acccagcccc caacccatca atgagataat ggcaacaaag ttcagccctt
      901 tgagcatatc cacgttttcg atgctctttt gtttgatcac ttccagcgag tcaccaaagc
      961 caccgacact gataatgacg ataaccggcg tcaggatcag ggcaaaaatc atcaggctgg
     1021 cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc aataaaggtg taaaggatcg
     1081 tcgacgcagc tcccgccaac agagccgttt cgtaactcat gccaaaggta ctttcaaaca
     1141 gacgcgcgcc tgccacaatg cccgaagcgc aataaatggt gaagaacagc aaaataacca
     1201 gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa acgcccggtg aaataatccg
     1261 gcaatgttaa ggcgttatta ttgtattcgg tatgcacacg caaccgcccg gccaccagct
     1321 tccagttgat ccacgcgcct aatgtcaggc caatggcgat ccagctttcg gaaatcccgg
     1381 aaagaaaaac cgcgccaggc aaccccatta acagccagcc gctcatatcc gacgcacccg
     1441 ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc cagaatatag tcgtcaaagt
     1501 ttttcgttga tcgccaggcg ataaacccaa tcaatatcat gccaaagata tagacacaaa
     1561 atgtcaccaa catcggtgtg ctaatagcca tctaaagtct ccaaaaaatt attatcggca
     1621 atgtcgaaac ttgccgttat atctgccacc ggaacggggt aacagagttt atgttttacc
     1681 agggcgaccg tatcctgccg gaagcgctgg ttattcacaa tcgatttaac acaccattta
     1741 cattaaattt tagtgctcag cgacactatt tttcatcagg ttgcactctc tcacattttt
     1801 tgcggttgca cctttcaaaa aggttaactg ccgcagagaa aaagtctgag ttatttttta
     1861 atcactgtca tatcgatttc ttttattaac atttcattca tttttaagct tgctacgcaa
     1921 gtcacattta acatggttgc acaaagttgc aacatcatgg atatttcacg ataacgttaa
     1981 gttgcacctt tcagaacaac aggagtaatg gcatgggaac caccaccatg ggggttaagc
     2041 tggacgacgc gacgcgtgag cgtattaagt ctgccgcgac acgtatcgat cgcacaccac
     2101 actggttaat taagcaggcg attttttctt atctcgaaca actggaaaac agcgatactc
     2161 tgccggagct acctgcgctg ctttctggcg cggccaatga gagcgatgaa gcaccgactc
     2221 cggcagagga accacaccag ccattcctcg actttgccga gcaaatattg ccccagtcgg
     2281 tttcccgcgc cgcgatcacc gcggcctatc gccgcccgga aaccgaagcg gtttctatgc
     2341 tgctggaaca agcccgcctg ccgcagccag ttgctgaaca ggcgcacaaa ctggcgtatc
     2401 aactggccga taaactgcgt aatcaaaaaa atgccagtgg tcgtgcagga atggtccaag
     2461 ggttattgca ggagttttcg ctgtcatcgc aggaaggcgt ggcgctgatg tgtctggcag
     2521 aagcgttgtt gcgtattccc gacaaagcca cccgcgacgc gttaattcgc gacaaaatca
     2581 gcaacggtaa ctggcagtca cacattggtc gtagcccgtc actgtttgtt aatgccgcta
     2641 cctgggggct gctgtttact ggcaaactgg tttccaccca taacgaagcc agcctctccc
     2701 gctcgctgaa ccgcattatc ggtaaaagcg gtgaaccgct gatccgcaaa ggtgtggata
     2761 tggcgatgcg cctgatgggc gagcagttcg tcacgggtga aaccatcgcg gaagcgttag
     2821 ccaatgcccg caagctggaa gagaaaggtt tccgttactc ttacgatatg ctgggcgaag
     2881 ctgcactgac tgccgcagac gcacaggcgt atatggtttc ctatcagcag gcgattcacg
     2941 ccattggtaa agcgtctaac ggtcgtggca tctatgaagg tccgggcatt tcaatcaaac
     3001 tgtcggcgct gcatccgcgc tacagccgcg cccagtatga ccgggtaatg gaagagcttt
     3061 acccgcgtct gaaatcactc accctgctgg cgcgtcagta tgatattggt attaacatcg
     3121 atgccgaaga ggccgatcgt ctggagatct ccctcgatct gctggaaaat ctctgtttcg
     3181 agccggaact ggcaggctgg aacggcatcg gttttgttat tcaggcttat caaaaacgct
     3241 gcccgttggt gatcgattac ctggttgatc tcgccacccg cagccgtcgc cgtctgatga
     3301 ttcgcctggt gaaaggcgcg tactgggata gtgaaatcaa gcgtgcgcag atggacggcc
     3361 ttgaaggcta tccggtttat acccgcaagg tgtataccga cgtttcttat ctcgcctgtg
     3421 cgaaaaagct gctggcggtg ccgaatctaa tctacccgca gttcgcgacg cacaacgccc
     3481 atacgctggc agcgatttat caactggcag gtcagaacta ctatccgggt cagtacgagt
     3541 tccagtgcct gcatggtatg ggcgagccac tgtacgagca ggtcaccggg aaagttgccg
     3601 acggtaaact taaccgaccg tgtcgtattt atgccccggt tggcacacat gaaacgctgc
     3661 tggcgtatct ggtgcgtcgc ctgctggaaa acggtgctaa cacctcgttt gttaaccgta
     3721 ttgccgacac ctctttgcca ctggatgaac tggtcgccga tccggtcact gctgtagaaa
     3781 aactggcgca acaggaaggg caaactggat taccgcatcc gaaaattccc ctgccgcgcg
     3841 atctttacgg tcacgggcgc gacaactcgg cagggctgga tctcgctaac gaacaccgtc
     3901 tggcctcgct ctcctctgcc ctgctcaata gtgcactgca aaaatggcag gccttgccaa
     3961 tgctggaaca accggtagcg gcaggtgaga tgtcgcccgt tattaaccct gcggaaccga
     4021 aagatattgt gggctttgtg cgtgaagcca cgccacgtga agtagaacag gcgctggaaa
     4081 gtgcggttaa taacgcgcca atctggtttg ccacgcctcc ggctgaacgc gcggcgattt
     4141 tgcatcgcgc tgccgtgcta atggaaagcc agatgcagca actgattggt attctggtgc
     4201 gtgaagccgg aaaaaccttc agtaacgcca ttgccgaagt gcgcgaagcg gtcgattttc
     4261 tccactacta cgccggacag gtgcgggatg atttcgctaa cgaaacccac cgtccattag
     4321 ggcctgtggt gtgtatcagt ccgtggaact tcccgctggc tattttcacc gggcagatcg
     4381 ctgccgcact ggcggcaggt aacagcgtgc tggcaaaacc ggcagaacaa acgccgctga
     4441 ttgccgcgca agggatcgcc attttgctgg aagcgggtgt accccctggc gtggtgcaat
     4501 tgctgccagg tcagggggaa accgtgggcg cgcaactgac gggtgatgat cgcgtgcgcg
     4561 gggtgatgtt taccggttca accgaagtcg ctacgttact gcagcgcaat atcgccagcc
     4621 gcctggacgc tcagggtcgc cctattccgc tcatcgctga aaccggcggc atgaacgcga
     4681 tgattgtcga ttcttcagca ctgaccgaac aagtcgtcgt ggatgtgctg gcctcggcgt
     4741 tcgacagtgc gggtcagcgt tgttcggcac tgcgcgtgct gtgcctgcaa gatgagattg
     4801 ccgaccacac gttgaaaatg ctgcgcggtg caatggccga atgccggatg ggtaatccgg
     4861 gtcgcctgac caccgatatc ggtccggtga ttgatagcga agcgaaagcc aatattgagc
     4921 gccatattca gaccatgcgt agcaaaggcc gtccggtgtt ccaggcggtg cgggaaaaca
     4981 gcgaagatgc ccgtgaatgg caaagcggca cctttgtcgc cccgacgctg atcgaactgg
     5041 atgactttgc cgaattgcac aaagaggtct ttggtccggt gctgcatgtg gtgcgttaca
     5101 accgtaacca gctaccagag ctgatcgagc agattaacgc ttccggctat ggtctgacgc
     5161 ttggtgtcca tacgcgtatt gatgaaacca tcgcccaggt cactggctcg gcccatgttg
     5221 gtaacctgta tgttaaccgt aatatggtgg gcgcagtggt tggtgtgcag ccgttcggcg
     5281 gcgaagggtt gtccggtacc gggccgaaag caggcggtcc gctctatctc taccgtctgc
     5341 tggcgaatcg cccggaaagt gcgctggcag tgacgctcgc gcgtcaggat gcagagtatc
     5401 cggtcgatgc acagttgaaa gccgcattga ctcagccgct aaatgcactg cgggaatggg
     5461 cagcaaatcg tccagaattg caggcgttat gtacgcaata tggcgagctg gcgcaggcag
     5521 gaacacaacg attgctgccg gggccgacgg gtgaacgcaa cacctggacg ctgctgccgc
     5581 gtgagcgcgt gttgtgtatt gccgatgatg agcaggatgc gctgactcag ctcgccgccg
     5641 tgctggcggt gggcagccag gtactgtggc cggatgacgc gctgcatcgt cagctagtaa
     5701 aggcattgcc atcggcagtc agcgaacgta ttcaactggc gaaagcggaa aatataaccg
     5761 ctcaaccctt tgatgcggtg atcttccacg gtgattcgga tcagcttcgc gcattgtgtg
     5821 aagccgttgc tgcgcgggat ggcgcaattg tttcggtgca gggttttgcc cgtggcgaaa
     5881 gcaatatcct tctggaacgg ctgtatatcg aacgttcgct gagtgtgaat accgctgccg
     5941 ctggcggtaa cgccagttta atgacgatcg gttaaacagg tagccggagg atgttacaac
     6001 ctcctccggc atctttaacg tggtcgaatc ccctcaataa taatccgctg cacgttttca
     6061 accgtttgat tgaaaaatat ctcatcgcgc aacgtcgcgc ctgtcaccgc ctccacctga
     6121 ggggcgaaat cggcgtaatg ttgagtggaa gcccaaatca tgaaaatcaa atgctgcgga
     6181 tcaatcggcg cgagtttgcc gcttttgacc caaccggcaa tcagcgcaga tttctcatca
     6241 attaatgcct tcaaatcgcc cgtcagttca tccattaaca gcggcgcgcc tgccagcatc
     6301 tccatacaga acaggcgcga agcctgcgga taatcgcggg agacttccag cttcagacgg
     6361 atgtactctt ttatcgccgc cagcggagcg aaatcttcac gaaacgcttt taacggtgcc
     6421 agccagatat cgagaatctg ccgcagcacg gcaatataca gcgcctcttt tgacggaaag
     6481 taatacagca gattggtttt tgaaacaccc gccaactctg cgatctgctc cagccttgtg
     6541 ccgtgaaaac cgaattgtga aaaagtgtcc agtgctgcgc taagaatcgc ttttttcttc
     6601 gcgcttactg cgcgcgaacg tttacccgtt gttttcactg cgccttgcgt catgcgctct
     6661 cccctcttgt agattggcaa caggatagca aaacccccgc ccggcctcga ccttctgcac
     6721 tctcatcgcg cacagatgca tgttttatgt gcaactgttt tgaccgttta gtccactttt
     6781 tatcagatat tttagataac gtaatattaa catattaata acattaactt tttaaaaact
     6841 ggcatccgct ttgcaaacaa gccaatacgc ggctgaaaag gatatgcagg atgcagcgcc
     6901 ccgtctgact ttcactttac gagacgaaga gaggttgatg atgaaaattg gcgtattcgt
     6961 acctattggc aacaacggct ggctcatttc gacccacgcg ccgcagtaca tgccgacctt
     7021 tgaactgaat aaagccattg tgcagaaagc ggagcattac catttcgatt tcgccctgtc
     7081 gatgatcaaa ctgcgtggct ttggcggcaa aactgagttc tgggatcaca accttgagtc
     7141 gttcaccttg atggcgggtc tggcagccgt gacctcgcgc attcagattt acgccactgc
     7201 tgccacctta acgttacctc cggcaatcgt cgcccgtatg gccgcaacca tcgactccat
     7261 ctctggcggg cgttttggcg tcaacctcgt gactggctgg caaaagcccg tgtatgagca
     7321 gatgggtatc tggcctggcg atgactattt ctcccgtcgt tacgactatc tcaccgaata
     7381 tgttcaggtg ctgcgcgacc tgtggggcac ggggaaaagc gatt
//
LOCUS       DAZOZY010000195         7368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZOZY010000195 DAZOZY010000000
VERSION     DAZOZY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.196"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            191..1255
                     /gene="ulaG"
                     /locus_tag="ACHURQ_003532"
     CDS             191..1255
                     /gene="ulaG"
                     /locus_tag="ACHURQ_003532"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR0825227.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1363..2118
                     /gene="ulaR"
                     /locus_tag="ACHURQ_003533"
     CDS             1363..2118
                     /gene="ulaR"
                     /locus_tag="ACHURQ_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR0825228.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2115..2864)
                     /gene="yjfP"
                     /locus_tag="ACHURQ_003534"
     CDS             complement(2115..2864)
                     /gene="yjfP"
                     /locus_tag="ACHURQ_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR0825229.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3046..3375
                     /gene="bsmA"
                     /locus_tag="ACHURQ_003535"
     CDS             3046..3375
                     /gene="bsmA"
                     /locus_tag="ACHURQ_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR0825230.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3524..3799
                     /gene="yjfN"
                     /locus_tag="ACHURQ_003536"
     CDS             3524..3799
                     /gene="yjfN"
                     /locus_tag="ACHURQ_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR0825231.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3916..5541)
                     /locus_tag="ACHURQ_003537"
     CDS             complement(3916..5541)
                     /locus_tag="ACHURQ_003537"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR0825232.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5625..6788)
                     /locus_tag="ACHURQ_003538"
     CDS             complement(5625..6788)
                     /locus_tag="ACHURQ_003538"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR0825233.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6791..>7368)
                     /locus_tag="ACHURQ_003539"
     CDS             complement(6791..>7368)
                     /locus_tag="ACHURQ_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR0825234.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
BASE COUNT         1927 a         1963 c         1798 g         1680 t
ORIGIN      
        1 ttatttattt aaattcattt agttaattta actacacatt ccattaaatc tttccacact
       61 caaaatcaac aacaacttcc ctggcaaaaa gtcaaaaaat ggtcattgcc ttcctgaaaa
      121 aatagtgtca ggattaatca aaaccaatca catattgatt cgaattgaac tatcatgaag
      181 gtaaatggcg atgagtaaag tgaaaagtat cacccgagaa tcctggatcc tgagcacttt
      241 cccggagtgg ggtagctggc tgaatgaaga aattgaacaa gagcaggtcg cccctggcac
      301 atttgcgatg tggtggctag gctgcaccgg gatctggttg aaatcagaag gtggcaccaa
      361 cgtttgcgtt gatttctggt gcggcactgg caaacaaagt cacggtaacc cgttaatgaa
      421 acagggtcac cagatgcagc gcatggctgg cgtgaaaaaa ctgcagccaa acctacgcac
      481 caccccgttt gttcttgatc cgtttgcgat tcgccagatc gacgcggtac tggcgactca
      541 cgatcacaac gatcatatcg acgttaacgt cgctgctgcc gtgatgcaga actgtgctga
      601 tgacgtacca ttcattggac cgaaaacctg cgttgatttg tggattggct ggggcgtacc
      661 gaaagagcgt tgcatcgtgg tcaaaccggg cgatgttgta aaagtgaaag acattgaaat
      721 tcatgcgctt gacgctttcg accgtactgc actgatcacc ctgcctgccg atcaaaaagc
      781 ggctggcgta ctgccagatg gcatggacga tcgcgcagta aactacctgt tcaaaacccc
      841 tggcggttcc ctatatcaca gtggcgattc ccactactct aactattatg cgaagcacgg
      901 taacgaacat cagatcgatg tggcgttagg atcgtatggt gagaacccgc gcggtattac
      961 cgacaaaatg accagcgccg atatgctgcg tatgggtgaa gccctgaatg cgaaagtggt
     1021 tatcccgttc caccacgaca tctggtcaaa cttccaggcc gatccgcaag agatccgcgt
     1081 gctgtgggag atgaaaaaag atcgtctgaa gtatggcttc aagccgttta tctggcaggt
     1141 tggcggcaaa tttacctggc cgctggataa agacaacttc gagtaccact atccgcgcgg
     1201 tttcgatgat tgcttcacta ttgaaccgga tctgccgttc aagtcattcc tgtaatctgt
     1261 cgtttatacc ggatagcgtg gagaagttcg cgctatccgg cgtagtattt cctagctatt
     1321 tcaaatatca tctaaaaaaa tcaaatttta tcggaatagc tcatgactga agcacaaaga
     1381 catcaaatcc tcctggaaat gctcgcacaa ttgggctttg tgaccgttga gaaagtcgtt
     1441 gagcgtctgg gaatttcgcc tgccactgcg cgacgcgata tcaataaact tgacgaacgc
     1501 ggcaaactga aaaaggtgcg caatggcgca gaagctatta cccaacagcg cccgcgctgg
     1561 acgccgatga atctgcatca ggcgcagaat cacgatgaaa aagtacgtat tgctaaagcg
     1621 gcctcgcagc tggttaatcc gggcgaaagc gtagtcatca actgcggctc caccgcgttt
     1681 ctgcttgggc gggaaatgtg tggcaagcca gtgcaaatca tcactaatta tctaccgctg
     1741 gcaaattacc tgatcgatca agaacatgac agcgtgatca ttatgggtgg acagtacaac
     1801 aaaagtcagt ccatcacttt aagcccgcag ggcagcgaaa acagtctcta tgccgggcac
     1861 tggatgttta ccagcggaaa agggctgacc gcagaagggt tgtataaaac cgatatgctg
     1921 acagcaatgg cagagcagaa gatgctgagc gtggtaggga aactggtggt actggttgat
     1981 agcagtaaga ttggcgaacg cgcgggaatg ctttttagcc gtgccgatca aatcgatatg
     2041 cttatcaccg gcaaaaatgc taacccggaa atcctgcaac aactggaagc gcaaggggtc
     2101 agcattctgc gtgtttaaag atgctggcgg aaaaatgtca cggcagcatc taacgcctca
     2161 ggcgtaatgc ggtgacgcac gcctggctgc catgaacagg ttagcagttt atcccgtccc
     2221 gtctcgctta aggcctgctg caaacgtagt gattcgtcgg caggcacaac gtcatcgtcg
     2281 aggccatgcc acagcagtag aggtctgtcc ccaagttgtt ccaggtggtt tgtcgcttcc
     2341 cactctgcca gcggcgcgat aatgttattg aattcattct gctgtgctgc cgtttcaggt
     2401 atcagcggtg gaaacagtga acgggcgagc gatgtaaaat agcccgatcc catcatgctg
     2461 gcggtacatt tcaccgtggg gtggcgagcg gtaattccca gtgccgtcat cgcgcccatc
     2521 gacgcgccac cgactgccag acggtcatca agcagccagt tttcttcggc tattgccgca
     2581 cgtaaagtag tgaattcctg catactttgt agcaagattt gccagaattg atttaaccgc
     2641 cgcgcttcgt cgccactaaa acggctaccg tgatcgggcg catccggcat gatcacccgc
     2701 aaaccagcct gcgccagcgc aacggcaaaa tagctataca ccaggctgga tgaagtaaag
     2761 ccgtgataaa aaattacgca cggtaacggg gtatcttttt gcccgacagg ataagcatga
     2821 agaacgggaa tatctgccag ctcgcgtgat tctatttcaa tcatctgtct ctcctttttg
     2881 tctggtatga catgaagaaa tccttagcac aagtttctgc tggcggtatt agttcataat
     2941 gttgagatgt gggttacgct ttcgtcacgt tttcattcga aaatcgcgta ccaggtaaca
     3001 attcgggaac attcccccaa agttaaatta ataaggcact acactatggt tagcaggaaa
     3061 cgtaatagcg ttatttaccg gtttgccagt ttattattgg tgttgatgtt aagtgcctgt
     3121 agcgcactgc aaggtacgcc acagccagca ccgccagtaa cggaccatcc gcaagagatt
     3181 cgccgcgacc agacgcaagg gttacagcga ataggtagcg taagcacgat ggttcggggt
     3241 tctccggatg acgcattagc agaaatcaga gcaaaagctg tcgctgcaaa agctgattat
     3301 tacgttgtcg tcatggtgga cgaaaccatc gtgacaggac agtggtattc acaagccatt
     3361 ttgtaccgta aataaatcag acaacttaca tagtcagctt tacattgctt tgcgtcccta
     3421 tgcgtttacc tgtgcacaat aaattaccgg ctggaagcct gggcgtttat taccgcgatg
     3481 gaatgccctg caacatgtgg ggaaacgaaa aatggagctg acgatgaaac aattacttgc
     3541 ctcaccctcg ctgcaattag tgacttatcc tgcgagcgcc acggcacagt ctgcagaatt
     3601 cgccagtgct gactgcgtga cgggcctgaa tgaaataggt cagatctcag ttagcaatat
     3661 ttcaggggat ccgcaggacg tggaacgtat cgtggcgtta aaggccgatg aacaaggtgc
     3721 gtcatggtac cgcattatta ccatgtacga agaccagcag cctgataact ggcgcgtaca
     3781 ggcgatactg tatgcatgag tgtcatctac agcaaatgaa caatgccgga tgcggcgcga
     3841 gcgccttgtc cggcctacaa ttttcacata tttcattgag ttaatcgaaa ccagcgtcgc
     3901 atcagtcgta tacgtttaca aacacactcc ccccgtcgcc cgcagcaata aatcattctg
     3961 gatctgctct gacaaccgta cgccgccgcg cgtatctaac atcacctgac accacgcctg
     4021 cgccattggc ggagaagcat atttcaacat ttgcgcaccg cagcccagca ggaatagctg
     4081 atgagtaatc tctcgcccca gttcttcagc tggcttacgc agctgctgct gtaaacgacg
     4141 aaccgcgcga tcaaaatagc gatcctgccc tttcacttcc acaaatgctt ccgacaataa
     4201 gtcgtatacg cccgcttgct tattgagaac gcgcaacaca tccaggcaca taatattgcc
     4261 ggaaccttcc caaatactgt ttaccggcat ctcccggtaa agccgcggca gctcgctctc
     4321 ctcgcaataa ccaatgccgc ccagcacctc catcgcttcg gccacaaacg gcatacctcg
     4381 tttgcagatc acaaattttg ccgcaggcgt aaataaacgc gcccacaggg cttctttggc
     4441 atcggcgcgc cggtcccacg ctcgcgcaag acgaaacagc aacgccgttt gcccttcaag
     4501 ctgaagtgcc atgcgactta agacatgacg cataaggggc tgttggatca atggattacc
     4561 aaaaacatgg cgttgatgtg catgataaat cgccagcgaa aacgcacggc gcatcatggc
     4621 atggctaccc agggcgcaat caaaacgcgt catccccccc attttcagga tcagacgaat
     4681 tccttcccct tcctgcccca ataaccagcc aatggcatcc tgaaactcca cttcacaact
     4741 ggcgttagag cgattaccca gcttatcttt cagccgttcg aggcgaatcg cgttgcgttg
     4801 cccgtcaggc aaaaagcgcg gcacaaaaaa acaggacaga ccgcccgtgg tctgcgccag
     4861 caccagatgc gcatcgcttt gcggaaccga gaaaaaccat ttatgcccca ccagccgata
     4921 agagccatct tccaaacgct ctgcacgggt ggtgttgctc atgacatcgg aaccaccctg
     4981 cttttccgtc attcccatgc caatcaacaa accgcgtttt tgcccacctg gcaataagtg
     5041 agaatcgtag cgatcgctca gcagcggcgt ggtccagtcc tgaaacggcg tgggtaacat
     5101 ctgtaacaac aatggcgtgg cggcaaaggt catcgttatt ggacataacg acccagcctc
     5161 aacctgcgca tgtaacataa aacgcgccgc gcgcgccaca aatgcgccgg agcgagcgtc
     5221 ttcttcccag gcaagattgt gcacccgatt ggtacataac acctgcatca gcaggtgcca
     5281 tgcgggatgg aaacgaacat cgtccagacg ccgcccctgg gcatcgtagc gcaacagttc
     5341 aggcgggttt acattcgcca gccgccccag ttcaagggat tcagccgttc ctaactgctg
     5401 accaatactg gctagaaaat cgctatccca gccagcacct tcacgcgtta ccgcttcgca
     5461 aagcgcgcca tcagacaggt ataagttgct gttatttaat ggtataggtt gattaaaaac
     5521 ggtgtgagtt tgccagtgca ctgtgtctcc ctccatcaat ggcagtcacc attagtatgg
     5581 ccactgccat ggattcatga cagaaagaga ttgctaaaac attcttatcc agcaatgtaa
     5641 tgcggaacga aacgtgaggt gtctttggtg atcagtgtgt tatcttcacg gatccccatt
     5701 ccgcacgctt catcatcgac aatccagcta tcgatgagtg tgtagctatc gccaaatcgc
     5761 ggcagaggtt gaaacgcctg gtagatcatc ggttcatcgg cgtaatcacc atcagcgtgg
     5821 tcaacgacgt tattctgacc gtcgaaaatg gtgacgtttc cgccttcgcg cgagtagatc
     5881 ggtttacgca catagctttc gccagcggca atctgcgatt tttcaccctc aaaccaggat
     5941 gcaagaaggt taggatgacc agggaagaag cgccaaagca gcggcattag ccctttatta
     6001 ctcaagatac ttttccataa cggctccacc cattgctcgc gacgcttgca cagcagcgga
     6061 ccgttatcgt cacgcatcat ccactccagc ggatacagct taaatgcacg ctggatgaca
     6121 ttatcatcaa gatcggtcag tacaccgccg acgcccaaac cgagatcttc aatgtagata
     6181 aaccgcgact cctgccctgc ctgctgggcg cagtcctgca agtacagcac ggtagtcctg
     6241 tcttcatcgg tgtcctgaca gcagcagaaa taaaacggct cccggctata aagctcgcta
     6301 aagcgtgaaa tcaggcgttc ctgaatggca ttgtactgat cggcatcacg cggaataatg
     6361 ccgctgcgcc gggcatcctc cagccacaac cactggaaat aagccgactc atacaacgaa
     6421 gtcggcgtat cggcgttgta ctccagcagc ttcaccggcg cattgccaca ccaggcaaaa
     6481 tccatccggc catacagcga aggatcgctg gcgtgccagc tttcggcgat cacatcccag
     6541 tacaacggcg ggatcgccag ttgcgtcagg atctcttcgt ctttaaccgc gcgatccacc
     6601 acctcaaggc acatctgatg cagttccgca gttggttttt cgatctgctc ttcaatctgg
     6661 cgcagagtaa aacggtaagc ccgactctcg tcccaataga tttcattgtc gatgatatga
     6721 aagtcgaaac cgttgtcagc ggcaatttgg tccagatccc gccgcacagg aacgttgtgt
     6781 ctcagcatga ttagcctccc caatgcccac gggcgctgga agaacgacca tagccgccgc
     6841 gcgaaacggt tgacgctttt ttggtggtaa agccgcctga agagtaagac tcttttttgc
     6901 cagaaccgga gcgccaggag taatcaccgg aagtgttgcg ccagacgggg cgggaagcaa
     6961 atgaggatcc gccgctgtta tagacgaacg gctcatcgcg atctttacga ataacccggc
     7021 ttaacaaaaa tccagaaacg accgggatcc aactctgttc aacattgtcg tagtagcaat
     7081 tttcgtactt agactgacag ttctgctgag tcatattctt cggaacatcg gcataaaatg
     7141 ccgttttggc gttgttccag ccacgcgcgc aaatgtcggc attattaccg tcatcaatac
     7201 aatcctgcac tgtcgcgtaa aacgttccgt cgccgtcgtt atcgacatcg ctactatcgc
     7261 cacaaccttt caatacgaaa aaagcggcac cgcccattag cgccaacgtt aagtattttt
     7321 gtgcgtagcg attgcgacgc ggttcaaaag gattattcgg tctaccaa
//
LOCUS       DAZOZY010000196         7314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZOZY010000196 DAZOZY010000000
VERSION     DAZOZY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.197"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..959)
                     /locus_tag="ACHURQ_003540"
     CDS             complement(<1..959)
                     /locus_tag="ACHURQ_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0825235.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1560..1808)
                     /locus_tag="ACHURQ_003541"
     CDS             complement(1560..1808)
                     /locus_tag="ACHURQ_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0825236.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2071..2166)
                     /locus_tag="ACHURQ_003542"
                     /pseudo
     CDS             complement(2071..2166)
                     /locus_tag="ACHURQ_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2163..2729)
                     /locus_tag="ACHURQ_003543"
     CDS             complement(2163..2729)
                     /locus_tag="ACHURQ_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR0825237.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3039..4811
                     /gene="aspS"
                     /locus_tag="ACHURQ_003544"
     CDS             3039..4811
                     /gene="aspS"
                     /locus_tag="ACHURQ_003544"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR0825238.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4929..5381
                     /gene="nudB"
                     /locus_tag="ACHURQ_003545"
     CDS             4929..5381
                     /gene="nudB"
                     /locus_tag="ACHURQ_003545"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR0825239.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            5410..6150
                     /locus_tag="ACHURQ_003546"
     CDS             5410..6150
                     /locus_tag="ACHURQ_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0825240.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHURQ_003547"
     CDS             6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHURQ_003547"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR0825241.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6708..7310)
                     /locus_tag="ACHURQ_003548"
     CDS             complement(6708..7310)
                     /locus_tag="ACHURQ_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR0825242.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1852 a         1640 c         1926 g         1896 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ctgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 cccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcacg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg cgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcggat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gagggggcaa tttttcctta
     1201 aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg tatcagtgaa
     1261 tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt attcttctgg
     1321 aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag
     1381 actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat gattggcggg
     1441 ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg gctggctggt
     1501 gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt acgtgctaat
     1561 tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa
     1621 gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct
     1681 tttacgatca cctctacatc gtcataacgt ttactgatgc gtcgggttaa ttcttccttt
     1741 aacgcatcca cagcaccggt tggcatttta gtcattttct ctttggctat gcagatttca
     1801 atacgcataa aaatccctct acactgtgtt tttatacagt attattttta actgtatgga
     1861 taaacagtgt caagagtgag ggaaagtgat tttttggggc atgcatgggg caaaaatatt
     1921 tgttgtgggg cattttgggg catgattggg acactttgtt acatatgaac tttgccgatt
     1981 ttcatatggc atggcaatcc aacgctttga ttattctaaa gaaaacacat gctcttgggc
     2041 gttctttagt gatttttaaa aatcccgtat cacgcagtta aagtggcggg catactcttc
     2101 aaggctggtg atccccaacc gcacccattt aggatgcgac cattgcggta gaccgatgta
     2161 aatcataacg cgtgaaggat ctcttccacg ctacgcacac gggcgatgcg cgggtagata
     2221 tgattaatgc tgttattgtg ttgctcggcg ctggcggcac tacaggcgtc ttcggcaatc
     2281 accagattaa agccaagttc ccaggcattg cgggcggtgg attcaacacc gatattggtc
     2341 gagatcccac ataacactat tgtatcgata cctcggcggc gcagttgcaa ctccagatcc
     2401 gtaccgtaaa acgcacccca ctgacgtttg atgatttcga tatcactgtc agttgcacct
     2461 aatgcagctg gatgttgcca ccagttttcg ggcaacactt tagcggggga gggggcatca
     2521 accggctgtt ttaatgcttc ggcgtaatcg gcagaccagc caactcgcac cagaaacacg
     2581 ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg cgcgattaac tacctcatcg
     2641 gcagtatgtg gaccaccggc aaaaggtaag atgccttctt gtaaatcgat caccaccaac
     2701 gcagtggttt tagcattaag ttcaagcatg ctgactcctg aaaaaacaaa atcgtgcctc
     2761 acaccttaat gcgattagcc gatggcggaa cgacaaattt tgttaatttt tatgagttcg
     2821 ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc aggcccgata taaagtggta
     2881 acgaggcgtt acagcgcggg ccaggctctt acggtgcggc agaatttcca gtataatagc
     2941 cgcctttttt cattcagttg tgacatacag ctaacgctgc gacttggtca cctgcgcagc
     3001 agggggcatc cgccggcagc taagttaagg gatatctcat gcgtacagaa tattgtggac
     3061 agctccgttt gtcccacgtg gggcagcagg tgactctgtg tggttgggtc aaccgtcgtc
     3121 gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg cgaaggtatc gtgcaggtat
     3181 ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc tgaactgcgt aatgagttct
     3241 gcattcaggt tacgggcacc gtacgtgcgc gtgacgaaaa aaatattaac cgcgatatgg
     3301 cgaccggcga aattgaagtg ctggcgtcct cgctgactat catcaaccgc gcagatgttc
     3361 tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg tctgaaatac cgctacctcg
     3421 acctgcgtcg cccggaaatg gcgcagcgcc tgaaaacccg cgctaaaatc accagcctgg
     3481 tgcgccgttt tatggatgac cacggcttcc tcgacatcga aactccgatg ctgaccaaag
     3541 ccacgccgga aggcgcgcgt gactacttgg tgccttctcg tgtgcacaaa ggtaaattct
     3601 acgcactgcc gcaatcccca cagttgttca aacagctgct gatgatgtcc ggttttgacc
     3661 gttactatca gatcgttaaa tgcttccgtg acgaagacct gcgcgctgat cgtcagcctg
     3721 aatttactca gatcgatgtg gaaacttctt tcatgaccgc gccgcaagtg cgtgaagtga
     3781 tggaagcgct ggtgcgtcat ttgtggctgg aagtgaaggg cgtggatctg ggcgatttcc
     3841 cggtaatgac ctttgcggaa gcagaacgcc gttatggttc tgataaaccg gatctgcgta
     3901 acccgatgga actgaccgac gttgccgatc tgctgaaatc tgttgagttt gcagtatttg
     3961 cggggccggc gaacgatccg aaaggccgtg ttgccgctct acgtgttccg ggtggcgcat
     4021 cgctgacccg taagcagatc gacgaatacg gtaacttcgt taaaatctac ggcgcgaaag
     4081 gtctggctta catcaaagtt aacgaacgcg cgaaaggtct ggaaggtatc aacagcccgg
     4141 tagcgaagtt ccttaatgca gaaatcatcg aagccatcct ggatcgtact gccgcgcaag
     4201 atggcgatat gattttcttc ggtgccgaca acaagaaaat tgttgccgac gcgatgggcg
     4261 cactgcgcct gaaagtgggt aaagaccttg gtctgaccga cgaaagcaaa tgggcaccgc
     4321 tgtgggttat cgacttcccg atgtttgaag acgacggtga aggcggcctg acggcaatgc
     4381 accatccgtt cacctcaccg aaagacatga ctgctgcaga actgaaagct gcaccggaaa
     4441 atgcggtggc gaacgcttac gatatggtca tcaatggtta cgaagtgggc ggtggttcag
     4501 tacgtatcca taatggtgat atgcagcaga cggtgtttgg tattctgggt atcaacgaag
     4561 aggaacagcg cgagaaattc ggcttcctgc tcgacgccct gaaatacggt actccgccgc
     4621 acgcaggtct ggcattcggt cttgaccgtc tgaccatgct gctgactggc accgacaata
     4681 tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc gtgtctgatg actgaagcac
     4741 cgagctttgc taacccgact gtactggctg agctgagcat tcaggttgtg aagaaggctg
     4801 agaataactg atatgactca aatacacgaa atcattcgcg ttgcatcaag gcggcaactg
     4861 agtgaactcc catgagcata gataactatg tgaatgggat gagcgaaggc agtcaacgaa
     4921 gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc gatcttagtg gtcatctatg
     4981 cacaagatac gaaacgggtg ctgatgttgc agcggcgtga cgatcccgat ttctggcagt
     5041 cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca agctgccatg cgcgaagtaa
     5101 aggaagaggt caccattgat gttgtcgctg aacaactgac cttaattgac tgtcagcgca
     5161 cggtagagtt tgaaattttt tcacatttac gtcatcgcta tgcgccgggc gtgacgcgta
     5221 atacggaatc atggttctgt cttgcgctcc cgcacgaacg ccaggtagtc ttcactgaac
     5281 atctggctta caagtggctt gatgcgcctg ctgccgcggc gctcactaag tcctggagca
     5341 accggcaggc gattgaacag tttgtaatta acgctgcctg aacaggcagg gctatctgga
     5401 gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa agctgcgcag
     5461 gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac cgcggctaag
     5521 ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg cggcggtgga taaagcactg
     5581 tctaacaaca tgacccgtga cacactgaac cgcgcaattg cacgtggtgt gggcggtgat
     5641 gatgatgcaa acatggaaac catcatctac gaaggttacg gtcctggcgg cacggcaatc
     5701 atgattgaat gtctgtctga caaccgcaac cgtaccgttg ctgaagtgcg tcatgcattt
     5761 agcaaatgtg gcggtaacct cggtactgat ggttccgttg cctatctgtt cagcaaaaaa
     5821 ggcgtgatct ccttcgagaa aggcgatgaa gacaccatta tggaagcagc actggaagca
     5881 ggtgctgaag acgttgtgac ctatgatgac ggcgcgattg acgtctacac tgcatgggaa
     5941 gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc tgaaagcaga cagcgcggaa
     6001 gtttccatga tcccgtccac caaagctgat atggatgcag aaaccgcacc gaaactgatg
     6061 cgtctgatcg atatgctgga agattgcgac gacgtgcagg aagtctacca taacggtgaa
     6121 atctctgatg aggttgcagc aactctctga tggggcctgc taaacagcaa aacggagacg
     6181 cgtgatggct attattctcg gcattgaccc gggttcgcgc gtgatcggct atggtgtcat
     6241 ccgccaggta ggcaggcaac tgtcctacct gggtagcgga tgcatacgca ccaaagtgga
     6301 tgatttaccg tctcgtctga agctcatcta tgcgggcgtg acggaaatca tcacccagtt
     6361 ccagccagat tatttcgcca ttgaacaagt ctttatggcc aaaaatgccg actcggctct
     6421 gaaactgggg caggcgcgcg gcgtggcgat tgtggcggcg gtgaatcagg aattgccggt
     6481 atttgaatac gcggcacgtc aggtaaagca aacggtggta ggtattggta gtgccgaaaa
     6541 aagccaggtg cagcatatgg tccgcacctt gctgaaactg cccgctaatc cacaggcgga
     6601 tgccgccgat gcgttagcta tcgcgattac ccattgtcac gtcagccaga atgcaatgca
     6661 gatgagcgaa tcgcggctga acctggcgag agggcgactg cgttaagtta taccgcctcg
     6721 gtcagttcag gctgaggcgt ttcaactccc tccgcgtgaa tcaacaccag acccggatga
     6781 tgccacaaac tggctggcgt gacggtttta cgctcccacg gaatagaacc taagaaccag
     6841 aatttccaga aagtgagttt tgcattcgga ttgctattta acagttctcc aaacgtttct
     6901 atttcaccca ccggaatgca tagcgcttct ttataaatat caaaaacaaa ttttgaacag
     6961 aactggcgcg aagattcgta tttaaaaccg gtgtggtaaa gtttgcgtag ccgggaggga
     7021 acctgttcaa caattcgttg tttttgttgt tctgtcagcc cagcgtctaa tcgctttata
     7081 gcatagcgtt gattagacga acgtttaata aaacgagata gcgtggtgat ggttgaaagg
     7141 ggaacccggc tttctgcaac caggaagtct tcaccgttat gaccgataat gatcccgacg
     7201 tgattactcc agcaatttga tgcagctgat atttgaccaa ataaggcagc acctatacat
     7261 gtaaagacga tatctccaat ttcatattca gcagggtaat taatattcac gttg
//
LOCUS       DAZOZY010000197         7191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZOZY010000197 DAZOZY010000000
VERSION     DAZOZY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.198"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHURQ_003549"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHURQ_003549"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR0825243.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHURQ_003550"
     CDS             complement(1672..2667)
                     /locus_tag="ACHURQ_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0825244.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHURQ_003551"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHURQ_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHURQ_003552"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHURQ_003552"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR0825245.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHURQ_003553"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHURQ_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR0825246.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..>7191)
                     /gene="emrK"
                     /locus_tag="ACHURQ_003554"
     CDS             complement(6108..>7191)
                     /gene="emrK"
                     /locus_tag="ACHURQ_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR0825247.1"
                     /translation="FSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHK
                     DTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQY
                     QQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLN
                     RQPQVVEAADATKEAWLALKRTDIRSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQM
                     WVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATG
                     NWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAEMPELASTVTSMPAY
                     TSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1974 a         1717 c         1666 g         1834 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa c
//
LOCUS       DAZOZY010000198         7130 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZOZY010000198 DAZOZY010000000
VERSION     DAZOZY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.199"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..4518)
                     /locus_tag="ACHURQ_003555"
                     /pseudo
     CDS             complement(1..4518)
                     /locus_tag="ACHURQ_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4538..4999)
                     /locus_tag="ACHURQ_003556"
     CDS             complement(4538..4999)
                     /locus_tag="ACHURQ_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR0825248.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6929)
                     /locus_tag="ACHURQ_003557"
                     /pseudo
     CDS             complement(5027..6929)
                     /locus_tag="ACHURQ_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1316 a         2187 c         1946 g         1681 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgacgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cctggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg gggggcggtt ttcctggtgg
     5581 taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc tacaatcact
     5641 tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca cgcctgtgcc
     5701 acacggatcc agcacgaact gtcttggtct gccgggttat aacggtccca gttgaacttc
     5761 acccgcacgc gaccatgttc gtcgcagaag atttcctcgc ctgccggtcc tgtcacgaca
     5821 gcgctctgcg ggccgtcgac cagcggtttc agcaacggct gtggtcgcca tgttctgtct
     5881 gccgggatca ccgcaaaatg gttctccagc gccgtccccg ctccctgccg tcctggcacc
     5941 gcctgtggct gttcgccgtg cagttcactt gccaccacct gccattcccg gttcaggttc
     6001 gcctgcggat gccccgtcag cacaattcgt cgtcccggcc atatctccgg cgagcgactc
     6061 attccccgcg cggtttctgc attgtttcgc cagccgtcca tctgccagcg ggcaaagttc
     6121 tgcccgtgag cacccttgaa acgtccgggg tagtcataca cttcatactg cgtgcgctgg
     6181 tagtcctggt gctggccttc ctgttcaaaa cgtccggccc agccggggcg tttaaaggtg
     6241 tagtctttgg tcaccacgga agaagggcgg atttgtgcgc tgtagcggaa ctggctgatg
     6301 cagagggtgc tcacctcggt acgggtgttc gggttccatg ggatttcaaa agattcgggc
     6361 agatggcgga ctgtgtcgca cagcaccagg ctctggtcgg tacttttgta agcatgctcc
     6421 tcataaaaga agatgccttc ctccgccgcc atccggcaca ggaaatcgta atcagtctca
     6481 ccgtactgga cacaaaactc acgggaagga tgcggctcgc tgaacagcgg actccattcg
     6541 gtcaccccgt tttcctgcaa catcgtgccg aggatgcttt tgatgtcctc gttctggaag
     6601 atacggaagt tctggcgcag accggcacgc cacagcggcg ggtgcacctt catgctgtac
     6661 agcatctggt ttttgtcgtt ctccccgagt tcaaaccagg tcaccacgcc tttcacccgg
     6721 cgctgtactt catcgccctg ccatatcgtc aggtaggcca ttttgtccag cacctgcgca
     6781 aattcaaggg agagaaactg ctggctgacc agggagagat cgagggaaaa gagcgaagag
     6841 agtgactggt tcagatgaaa ggaaaccacc gcaaaagcgt ccggcggcag gccgtccact
     6901 tccagtgtga aacgtaatcc ggttgacata gcaacctcca gtaaaatgaa taatgatccg
     6961 taccgtacaa ctgcggataa tgtgataagc gggacgactt cagacgttat tagggaagga
     7021 acagaaaatg ctatggttat gtgagaattt tgtttaaata ttactttaca gaaacgggtg
     7081 tctgttcagt caaaaaaata atcacaagct caacctcttg tttcttggcg
//
LOCUS       DAZOZY010000199         7121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZOZY010000199 DAZOZY010000000
VERSION     DAZOZY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.200"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..435
                     /locus_tag="ACHURQ_003558"
     CDS             <1..435
                     /locus_tag="ACHURQ_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR0825249.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="ACHURQ_003559"
     CDS             518..1420
                     /locus_tag="ACHURQ_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR0825250.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHURQ_003560"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="ACHURQ_003560"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR0825251.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHURQ_003561"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="ACHURQ_003561"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR0825252.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHURQ_003562"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="ACHURQ_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR0825253.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="ACHURQ_003563"
     CDS             complement(5330..6037)
                     /locus_tag="ACHURQ_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR0825254.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHURQ_003564"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="ACHURQ_003564"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR0825255.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="ACHURQ_003565"
     CDS             complement(6895..7098)
                     /locus_tag="ACHURQ_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR0825256.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1990 c         1855 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggacgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAZOZY010000200         7004 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZOZY010000200 DAZOZY010000000
VERSION     DAZOZY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.201"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..268
                     /gene="rutC"
                     /locus_tag="ACHURQ_003566"
     CDS             <1..268
                     /gene="rutC"
                     /locus_tag="ACHURQ_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0825257.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
     gene            276..1076
                     /gene="rutD"
                     /locus_tag="ACHURQ_003567"
     CDS             276..1076
                     /gene="rutD"
                     /locus_tag="ACHURQ_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR0825258.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            1086..1676
                     /locus_tag="ACHURQ_003568"
     CDS             1086..1676
                     /locus_tag="ACHURQ_003568"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR0825259.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            1687..2181
                     /gene="rutF"
                     /locus_tag="ACHURQ_003569"
     CDS             1687..2181
                     /gene="rutF"
                     /locus_tag="ACHURQ_003569"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR0825260.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            2202..3530
                     /gene="rutG"
                     /locus_tag="ACHURQ_003570"
     CDS             2202..3530
                     /gene="rutG"
                     /locus_tag="ACHURQ_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR0825261.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3788..3961)
                     /locus_tag="ACHURQ_003571"
     CDS             complement(3788..3961)
                     /locus_tag="ACHURQ_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR0825262.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHURQ_003572"
     CDS             4334..4930
                     /gene="wrbA"
                     /locus_tag="ACHURQ_003572"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR0825263.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            4951..5178
                     /locus_tag="ACHURQ_003573"
     CDS             4951..5178
                     /locus_tag="ACHURQ_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR0825264.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHURQ_003574"
     CDS             complement(5216..6457)
                     /gene="agp"
                     /locus_tag="ACHURQ_003574"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR0825265.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(6751..>7004)
                     /locus_tag="ACHURQ_003575"
     CDS             complement(6751..>7004)
                     /locus_tag="ACHURQ_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825266.1"
                     /translation="EVKNFLTALSDSICSLFIVYSNVASISLGNESFSTDDDLEYGYL
                     INTGNHYDVYLPPELFAQAYELNNKERNAQIDFLTRYAI"
BASE COUNT         1580 a         1781 c         1874 g         1769 t
ORIGIN      
        1 taacgtgctg tttgccgatg acccaaaggc gcaaacccgc cacgttctgg aaactatccg
       61 caaggtgatc gagacggcgg gtggcacgat ggcggatgtg accttcaaca gcatctttat
      121 taccgactgg aaaaattacg ccgcgattaa cgaaatctac gccgagtttt ttccgggtga
      181 taaaccggcg cgattctgca ttcagtgcgg actggtaaaa cctgacgcgc tggtggaaat
      241 cgccacaatt gcgcatatcg ccaagtgagg ccgcgatgaa actttcactc tcacctcccc
      301 cttatgctga tgcgcccgta gtggtgttga tttcgggtct tggcggtagc ggcagttact
      361 ggttaccgca actggcggtg ctggagcagg agtatcaggt agtctgttac gaccagcgcg
      421 gcaccggcaa taatcccgac acgctggcag aagattacag tatcgcccag atggcagcgg
      481 aactgcatca ggcgctggta gccgcaggga ttgagcatta cgcggtggtc ggccatgcgc
      541 tcggtgcgct ggtgggaatg cagctggcgc tggattatcc cgcgtcggta actgtgctga
      601 tcagcgttaa cggctggcta cgaataaacg cccatacgcg ccgctgtttt caggttcgcg
      661 aacgattact gtatagcggc ggcgcgcagg catgggtgga agcgcagccg ttgttcctct
      721 atcccgccga ctggatggcg gcccgcgcac cttgccttga ggcagaagac gcgctggcac
      781 tggcgcattt tcagggcaaa aataatttac tgcgtcgact taacgccctc aaacgcgctg
      841 actttagtca ccatgcggat cgcatccgct gcccggtgca aatcatctgc gccagtgatg
      901 atctgctggt gccatcagca tgttccagtg agcttcatgc cgccctgccc gatagccaga
      961 aaatggtgat gcgctatggc ggacacgcct gcaacgtgac cgaccccgaa acgtttaatg
     1021 ctctgttact caacgggctt gccagcctgt tacatcaccg tgaagccgcc ctgtaaggaa
     1081 ttgctatgaa cgaagccgtt agcccaggtg cgcttagcac cctgttcacc gatgcccgca
     1141 ctcacaacgg ctggcgggag acacccgtca gcgatgagac gttacgggag ctttatgccc
     1201 cgataaaatg ggggccgaca tcagctaact gttctccggc acggatcgtg tttatccgca
     1261 ctgtagaagg aaaagaacgt ctgcacccgg cgctttccag cggcaatcta caaaaaaccc
     1321 tgaccgcgcc cgtcaccgct atcgttgcct gggacagtga attttatgaa cggttaccac
     1381 tactgtttcc ccacggtgat gcccgcagtt ggtttacctc cagcccacaa cttgccgaag
     1441 aaacagcgtt tcgcaacagc tccatgcagg cggcctatct gatcgtcgct tgccgtgcgc
     1501 tgggactgga taccggcccg atgtcgggtt ttgaccgtca acacgttgac gacgcctttt
     1561 ttgcgggcag cacgctgaag agcaatctgc tgattaatat cggctatggc gatagcagca
     1621 aactttttgc gcgcctgcca cgtctgtcct ttgaagaagc ctgcgggctg ttgtaaggag
     1681 caaactatga acatagttga tcaacaaacc tttcgcgatg cgatgtcctg tatgggcgcg
     1741 gcggtcaata tcatcaccac ggacggtcca tccgggcgcg ccgggttcac cgccagcgcc
     1801 gtctgcagcg tgaccgacac gccgcccaca ttactggtgt gcctgaatcg tggggcgtcc
     1861 gtctggccgg tattcaatga aaatcgaacg ctgtgtgtaa atacactgag cgccgggcag
     1921 gagccgcttt caaatctttt tggcggcaaa acgcccatgg aacaccgctt tgccgccgcc
     1981 cgctggcaga cgggtgtgac cggatgcccg caactggaag aggctctggt ttcgtttgac
     2041 tgccgcatca gccaggtggt gagcgtcggc acccacgaca ttctgttttg cgccatcgaa
     2101 gcgattcatc gtcacaccac accctacggg ctggtgtggt tcgatcgcag ttatcacgcg
     2161 ctgatgcgcc ctgcttgtta acctccttaa ggagacagct gatggcaatg ttcggttttc
     2221 ctcactggca gttaaaatcg acctctacag agagcggcgt ggtcgcgccg gatgaacgat
     2281 tgccgtttgc tcagacggca gtaatgggcg ttcaacacgc ggtggcgatg tttggcgcga
     2341 cggtgttaat gccgattctg atggggctgg atcccaatct ttccatttta atgtcgggga
     2401 tcggcacgtt actgtttttc tttattaccg gcgggcgcgt ccccagttat ctcggctcca
     2461 gcgcggcttt tgtcggcgtg gtgattgccg cgacgggctt taacggtcag ggcattaacc
     2521 cgaatatcag catcgcactg ggcgggatta tcgcctgtgg gctggtttat accgtcattg
     2581 gcctggtggt gatgaaaatt ggtacacgct ggattgaacg actaatgccg ccggtggtga
     2641 cgggtgcggt ggtgatggcg attggcctga acttagcgcc tatcgcagtg aaaagtgtat
     2701 cggcttcggc ctttgatagc tggatggcgg taatgacggt gctgtgtatt gggctggtgg
     2761 cggtgtttac ccgtggcatg atccagcggt tattgattct cgtaggttta attgtcgcct
     2821 gtctgctgta tggcgtgatg accaacgttt tagggctggg aaaagccgtc gattttactc
     2881 tcgtcagcca tgctgcctgg ttcggtttgc cacatttttc cactcctgct tttaatagtc
     2941 aggcaatgat gctgattgcg cccgtggcgg tgattctggt ggcggaaaac ttaggtcatc
     3001 tcaaggcggt cgccgggatg accggacgca atatggaccc gtatatgggg cgagcattcg
     3061 tcggtgatgg tctggcaacg atgctttccg gctctgtcgg cggtagcggc gtcaccacct
     3121 atgctgaaaa tatcggtgtg atggccgtga caaaagtcta ctcgacgctg gtgtttgttg
     3181 ctgctgccgt catcgccatg ctgttgggct tttcgccgaa atttggcgcg ctgatccata
     3241 ccattccggc cgcggtgatt ggtggtgcat caattgttgt gttcgggctg attgccgtcg
     3301 caggcgcaag gatctgggta caaaaccgtg tcgatttaag ccagaacggt aatttgatta
     3361 tggtcgcagt aaccttagtg ctgggcgcgg gtgattttgc tctcacgctg ggcggtttta
     3421 cgttgggagg gatcggtaca gccacctttg gcgcgatttt actcaatgcg ttgttaagta
     3481 gaaggttggt tgacgttcca ccgccagaag ttgttcatca ggagccataa acagaaaacc
     3541 gccggggtta cccggcggtg cagattgttg acaacgtacg cattgttcat gccggatgcg
     3601 gcgtaaacgc cttatccggc ctacaaaaca gtgcaaattc aatatattgc aatcttctgg
     3661 taggcctgat aagcatcgcg catcaggcaa gcttacattt gtcatcagta tcaaccaccg
     3721 ggtaaccggc ggtgatggct ttttgacgag ctacagacaa aaatgcattc gtcattgatt
     3781 agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag
     3841 atgcgcgctg cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg
     3901 cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca
     3961 ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg
     4021 gctgccatat tttcgctacg tgggactaag cttagtgaac tgacgcgggt agacccgcgt
     4081 ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa taattatcca
     4141 taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa
     4201 attacgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga
     4261 aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga
     4321 ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga
     4381 aacgatggca cgcgcagtcg ctgagggtgc aagcaaagtc gatggcgcag aagttgtcgt
     4441 taagcgtgta ccggaaacca tgccgccgca attatttgaa aaagcaggcg gtaaaacgca
     4501 aactgcaccg gttgcaaccc cgcaagaact ggccgattac gacgccatta tttttggtac
     4561 acctacccgc tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg
     4621 cctgtgggct tccggcgcac tatacggaaa actggcgagc gtctttagtt ccaccggtac
     4681 tggcggcggt caggaacaaa ctattacttc aacctggacg acccttgcgc atcacggcat
     4741 ggtgattgtc cccattggct acgcagcgca ggaattattt gacgtttcac aggttcgcgg
     4801 cggtacgccg tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc agccaagcca
     4861 ggaagaactg tctattgctc gttatcaagg ggaatatgtc gcaggtctgg cagttaaact
     4921 taacggctaa tcttcaacag gaggatacgc atgccaactc aagaagcgaa agctcatcac
     4981 gtcggtgaat gggcatctct gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa
     5041 gtcgccgggt acgacgaaaa aatggcagaa aagatttggg aagaaggtag cgatgaagtg
     5101 ttagtcaaag cctttgctaa ggccgataaa gattcgcttt tttggggcga acagaccatc
     5161 gaacgtaaaa acgtttaacc agcgactccc ccgcttctcg cgggggagtt ttctgttatt
     5221 tcaccgcttc attcaacacg ctatcaaatt tatccatcgg gcagaaaccg ttggcatcta
     5281 tcgggcaacc gcttaattcc agcgtcacac gctgagcagg tgcctgcagg gttaacgcat
     5341 cggcattacg taactgttcc gcactctgat acacatattc aattttcatc aaatcgcgat
     5401 tggctttgct gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt
     5461 tctggtcatg caactgatac ggtttgaaat ccagcgccgt taacagagag gcaatgttgg
     5521 agtcgtgccc aaccaacact gtaattttcg gtgcgctggt gcgatcggtg accagagctt
     5581 tgtcgatata actgaccagc ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca
     5641 ggctgtcctg gtagccgttt ttaagcttcg acaacacctt ccactgctgg tcagatttga
     5701 tttctcccca ggccacctga tccatcggaa aaccttcgta atattgcaaa gtaaacgcat
     5761 ctaccagcga gttgccgact ttcagcggcc cggaaacacc tggttcttgt tgatacttgg
     5821 cgctaaaggt atttttgcca tccaccagcg aacactgttg tttctcttta caggcagggg
     5881 aatctttata gttaacgatt ttttccagta gctggtagct gtcggtaagc tggagtttgc
     5941 tgagctcttt ctccattgcc gccaccgcct gttcactgaa tgcggcggaa tcatcggtga
     6001 tcaccgggtt aaaggttggg tccatagtgc ccattttttc ctggtgatgc acaggaatat
     6061 cacacccggg gaatgcgccg gtaataaaga actgtgcggt cgcaacggta cgttgcagac
     6121 tattggcata ggcataaacg gtgtccggcg gcgggcattc ccccgatttc accatcccct
     6181 gctctgccag ccattcacgc atgtaatggc ccatatacac ttcgagcacg ccacctttgg
     6241 tggtgagttg cccaccgggg acgtcccatt ctggccattt attcggcgtc gactgctcca
     6301 gcacactgcc attgttcgcc agcggcgcac gtaagttatg gcggctcatc atgagcactt
     6361 gctgtagctg atagccttcc ggtacggttt gtgcctgagc atttgaagcg agtaaaacta
     6421 tccctgccac agttgcggcg attagcgttt tgttcattcc tggcacctct tttgttatca
     6481 atcattaaag agtgtgacag aaatatgact tttttccggg aacaggttcg caaaatggct
     6541 tgttgaacgc ctgtttttct atatatatct tcaaaaacag gcaaggtttg acgcaaacta
     6601 tgcctgatta gcccctgcct cgcgtacgcc gctgataatt gcacctgtgg ctaaacagaa
     6661 tatcagcggc gttactaata cagaggcaat agtaccaata ataagagaag tgaggctaat
     6721 aacaatggtt tttagcacat gcctttgtca ttaaattgca taacgagtta aaaaatcaat
     6781 ttgcgcattc ctttccttat tgtttaactc gtaagcctga gcaaaaagtt caggagggag
     6841 gtaaacgtca taatgattgc cagtgtttat taaataccca tactcaaggt cgtcatctgt
     6901 tgaaaaggat tcattcccta aggagattga tgccacattg ctgtaaacaa taaataatga
     6961 gcaaatgcta tctgaaagag cagtgaggaa atttttcacc tcat
//
LOCUS       DAZOZY010000201         6973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZOZY010000201 DAZOZY010000000
VERSION     DAZOZY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.202"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(422..907)
                     /gene="sixA"
                     /locus_tag="ACHURQ_003576"
     CDS             complement(422..907)
                     /gene="sixA"
                     /locus_tag="ACHURQ_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR0825267.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1110..3254)
                     /gene="fadJ"
                     /locus_tag="ACHURQ_003577"
     CDS             complement(1110..3254)
                     /gene="fadJ"
                     /locus_tag="ACHURQ_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR0825268.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3254..4564)
                     /gene="fadI"
                     /locus_tag="ACHURQ_003578"
     CDS             complement(3254..4564)
                     /gene="fadI"
                     /locus_tag="ACHURQ_003578"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR0825269.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4744..5028)
                     /locus_tag="ACHURQ_003579"
     CDS             complement(4744..5028)
                     /locus_tag="ACHURQ_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR0825270.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5400..6740
                     /gene="fadL"
                     /locus_tag="ACHURQ_003580"
     CDS             5400..6740
                     /gene="fadL"
                     /locus_tag="ACHURQ_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR0825271.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1769 a         1845 c         1640 g         1719 t
ORIGIN      
        1 acggaataag tatttagaaa aaatagaaag tatatcaata atgaatatca acagcttaaa
       61 aaacaaacaa tgaacatata atgcgattgg catttatcca cttttgacaa tataatatat
      121 aaacactata gccagaattt aatcaagttt caattacatc ctgcctgatt attcaactct
      181 attttctcta ttatctgtat taataaatga caatgagtgt caaacagaca ttattcattt
      241 caacatgtaa ctttgggcaa aatcaagata cgcgcagaag aataatttcc gaatgaaagt
      301 agaaagtgtt ttatttatag atggggagag tcttcgatag tagtgagatt gacagatatc
      361 cgctgcctga tgagcaatta tgcccaatga atctacctca ttgggcataa tttggaactc
      421 atcagatagc ttttgccatc ttcagattac acgggctcat ctgccagttg aatgtgccgt
      481 ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata ggcggcgttt
      541 cgcccggaca taactcggca accagatacc cgactaacgg taagtgagag atcaccagta
      601 ccgaagcaac accttcattg gtcaacgcct gcaaataggc actgaccaga ccaacatcgc
      661 cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga caatccccta
      721 cttcttccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt tcgatttcca
      781 ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaacca ttagtggtca
      841 gaggacgaac cgaatcactg gcggcatcaa gggctgcgtc gccgtgacgc atgataaaaa
      901 cttgcatatt gcaccgcttt tgttaaccag attcgtccgt tttctttcat ctgaaaccag
      961 agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa cgctgttttt
     1021 tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca ctccgccatt
     1081 cagcgcggat tcatatagct ttgaccttct tattgaaggt cagttgcagt tgttttccaa
     1141 aaactttccc cacgcgcgcc catctcgacc aaacgctcgc aaggggtaaa acgggaacca
     1201 tactgcgtgg ccagtcgttg cattattgca accacttcgc ccgcgccgag agaatcgata
     1261 tagcggaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc gccaatatcc
     1321 ccgtcacgca cgctacggat aacctgctca tcaacacaac gtactgcttc attcagcatc
     1381 aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc ttgtgcgcca
     1441 atgagcgggt aaatggcggg atcgacctgt tttttgcttt tacgcccttt ctgaccataa
     1501 agatagaaac cccgaccatt ttttctgcct ttgcgatcgt cgttcaaaat tgaagaaaca
     1561 acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg aataatttta
     1621 gtcccggtgt cgattcctac ctcatccaaa agttggattg ggcctaccgg aaaaccaaat
     1681 ttcactagcg cggcatcaat gtgctctacc cgttcacctt cggtcagcat gcggatagct
     1741 tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc acgcacgaca
     1801 atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt ttgcgccgat
     1861 gtacccgcat gaggaataat ctctaccagc ggcatttttt ccacaggact gaagaaatgc
     1921 aggccaataa cttgctcagg tcgcgcggcg tgagcggcga tatcaccaat cggtaaagat
     1981 gacgtgttcg aagcaaaaat ggtatgagcg gcgcaatttt gctccacttc cgccaccatc
     2041 tgttgtttta attcgagatt ttcaaacacc gcttcaataa tcagatcgcg atgggcaaag
     2101 ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc gctggctttg
     2161 agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag cgcatgattt
     2221 atgccctgcg ggttgatatc tttaattctg accggaagcc ccgctttaca agcagtgaca
     2281 taagcaatac cgccgcccat caagccacca cctaaaattc ccacgctgtt taatggcgca
     2341 ggcggtgcat cactaccggg atctttcttc acgtccgtac tggcaaaaaa gatactacgc
     2401 aaagcttgcg attgcggcgt catcgccagt tcgccaaagg cgcgagcttc agcgtcatag
     2461 ccgctgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg ttctgtcgcc
     2521 ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa cagcagcgca
     2581 cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga agatgggcga
     2641 tccttcttcg ccagctcaac agcggcttcc agcaaaatgg agtgcggaac aacgtcatcc
     2701 accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag aatcatctct
     2761 aatgctgtac tgacgcctat cagacgcggt aaacgctggg tgccgcctga accgggtaac
     2821 aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt acaaacgcga
     2881 ccgtggcacg ccagcgccag ctccagccca ccgcccaggc aagcgccatg aatagccgcg
     2941 ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg ccgcgccagt
     3001 gcttccgctt cttgcgccgt tttgcagttg ccaatcatgt tgatgtctgc tccggcgata
     3061 aaattgtccg gtttcgcgga gataaacacc acgccgcgca actctttgtt ttcacggagt
     3121 tgcttaataa tggcgcgcac ctgcgaggca aattccgcct tcagggtatt cattttctca
     3181 tccggtacat cgatggtgat gatagcaatg ttgtccagac gaacattaag agtaaacgct
     3241 gatgccattt ccattattcc gcctccagaa ccattgccgc gccaagccca ccggcagcac
     3301 aggcggtaac taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat gtctgggtaa
     3361 tcatccgcgc gccggtcgcc gcgaagggat gcccgtaagc aatcgaaccg ccaagcacgt
     3421 taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagtact tcacgagcaa
     3481 aacgttcact acccagcaac tgaatattcg ccagtgtctg agctgcaaag gcttcgtgca
     3541 tatcgatcaa tgtcagttcg ctcatcgtca aaccggcacg ttccagcgcc agcggtgttg
     3601 accaggctgg accgagcaac atgtcctgcc agacatcaat cgcagtaaat gcgtagctgc
     3661 gcagataccc cagcggcacc agccctaatt ctttcgcccg ggattcagtc atcaggatca
     3721 ccgctgccgc gccatcggtc agcggcgtac tgtttgccgc cgttaccgtt ccgtgtttgc
     3781 gatcaaacgc cgggcgcagc tttgcgtaat cggcaagcga ggaattaccg cgaatattgt
     3841 tgtcttcgac aagcggttgt ttataaggag ggataaaggc agtcatcacc tcttctttga
     3901 gttttccgtc tgaccatgcc tgagcggcac gctgatgcga acggtgcgct aatgcatctt
     3961 gctgttctcg ggtgatgccg taggttttcg ccatttgttc ggcggtgtcg cccatccgca
     4021 agccagtaga atattctgct accgcaggcg gtacgggcat taagtcgcgc aaacgcaggc
     4081 gagagaagag tttcaggcgc tggctcatgg ttcgagcttt gttgacatca accagcacgc
     4141 gcgccagttt tttactgacg ccaattggca atacggaaga ggaatctgcc ccaccggcaa
     4201 tccccgctcg gatagttccc gccatcaggc tttctgcgac gtttgcaacc gcctggaaac
     4261 tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt ccggtaccga
     4321 gaacaatttc acgcgcaatg ttgggggctt caggcatttg tacgacctga ccaaagacca
     4381 gctgttcaat cacttcagcg gggatctcgc tgcgtgccag cagttcgcct accaccatct
     4441 tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctggcgggca aaaggcgtac
     4501 gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagcggt aaaacctgac
     4561 ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat agtcttaact
     4621 atttttttac atttagccaa gcggagaaaa aggaaagtgg gagctatgac acagtgaaga
     4681 caggagaaag aaaagaaaac gcccctgcca gcaactgaca ggggcgaacg tacccgagag
     4741 gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa aagtgaaatc
     4801 gatatccagg cgcacaccgt cagactcttc agtgaaagtc ggggagattt tgcacggttc
     4861 ggattccacg ctacgggctt tttcagtcag cgccgccagc gtttgttctg cttctgcgcg
     4921 gtttgcaaac acgcggctgt aagacgcggt gcagtcggag ttgtccataa tggtgccaac
     4981 atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg ttgatttcct
     5041 ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt ccggaaagtg
     5101 ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac ttaaaaatga
     5161 tctaaaacaa aattcacccg aatccgcgag tgcgcaacct ccaaattttg ccagctggat
     5221 cgcgtttctt agatcatatt tgaaaaaaga tggaaacata cttgcagcat tccagctggt
     5281 ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc ctacacttcg
     5341 cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag gttatggtca
     5401 tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca cttatctcca
     5461 cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc ctgggccggg
     5521 cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgttagccgt aaccccgcat
     5581 tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt gacccggatg
     5641 tgaatatcag cggaacgtct ccgtctggtc gtagcctgaa agccgataac atcgcgccta
     5701 cggcatgggt tccgaacatg cactttgttg caccgattaa cgaccaattt ggttggggcg
     5761 cttctattac ctctaactat ggtctggcaa cagagtttaa tgatacttat gcaggcggct
     5821 ctgtcggggg tacaactgac cttgaaacca tgaacctgaa cttaagcggt gcgtatcgct
     5881 taaataatgc atggagcttt ggtcttggtt tcaacgccgt ctacgctcgc gcgaaaattg
     5941 aacgtttcgc aggcgatctg gggcagttgg ttgctggtca gattatgcaa tctcctgccg
     6001 ggcaaactcc tcaagggcaa gcattggcag ctaccgccaa cggtatcgac agtaatacca
     6061 aaatcgctca tctgaacggt aaccagtggg gctttggctg gaatgccgga atcctgtatg
     6121 aactggataa aaataaccgc tatgcactga cctaccgttc tgaagtgaaa attgacttca
     6181 aaggtaacta cagcagcgat cttaatcgtg cgtttaataa ctacggtttg ccaattccta
     6241 ccgcgacagg tggcgcaacg caatcgggtt atctgacgct gaacctgcct gaaatgtggg
     6301 aagtgtcagg ttataaccgt gttgatccac agtgggcgat tcactatagc ctggcttaca
     6361 tcagctggag tcagttccag cagctgaaag cgacctcaac cagtggcgac acgctgttcc
     6421 agaaacatga aggctttaaa gatgcttacc gcatcgcgtt gggtaccact tattactacg
     6481 atgataactg gaccttccgt accggtatcg cctttgatga cagcccagtt ccggctcaga
     6541 atcgttctat ctccattccg gaccaggacc gtttctggct gagtgcaggt acgacttacg
     6601 catttaataa agatgcttca gtcgacgttg gtgtttctta tatgcacggt cagagcgtga
     6661 aaattaacga aggcccatac cagttcgagt ctgaaggtaa agcctggctg ttcggtacta
     6721 actttaacta cgcgttctga taacgcgttc gcctggataa agtcacctgc atagcaggtg
     6781 actttaactc ccccacttca ccgaaagtag tgtcctcatt gcttacctcc tgagttttgc
     6841 aaacagcctg ttggcagctt gccctttcaa atcaataagc ggtatccaca gacaaacctg
     6901 aagggaaagg catattttca ggcgttctgc tcgtccttct caaagagttt acttttctgc
     6961 atttccagga tac
//
LOCUS       DAZOZY010000202         6874 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZOZY010000202 DAZOZY010000000
VERSION     DAZOZY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.203"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            462..809
                     /locus_tag="ACHURQ_003581"
                     /pseudo
     CDS             462..809
                     /locus_tag="ACHURQ_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            862..1257
                     /locus_tag="ACHURQ_003582"
     CDS             862..1257
                     /locus_tag="ACHURQ_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR0825272.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1298..2041
                     /gene="cmoA"
                     /locus_tag="ACHURQ_003583"
     CDS             1298..2041
                     /gene="cmoA"
                     /locus_tag="ACHURQ_003583"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR0825273.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2038..3009
                     /gene="cmoB"
                     /locus_tag="ACHURQ_003584"
     CDS             2038..3009
                     /gene="cmoB"
                     /locus_tag="ACHURQ_003584"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR0825274.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3174..5603)
                     /gene="torZ"
                     /locus_tag="ACHURQ_003585"
     CDS             complement(3174..5603)
                     /gene="torZ"
                     /locus_tag="ACHURQ_003585"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR0825275.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5628..6728)
                     /locus_tag="ACHURQ_003586"
     CDS             complement(5628..6728)
                     /locus_tag="ACHURQ_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR0825276.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1609 a         1754 c         1613 g         1898 t
ORIGIN      
        1 ttccttatga gactggttaa tagagcgcta tgaagattca ggagggtaac tataaccatc
       61 actttctaac tttcacgtag gaaatcgtta agtttcttga tagttacacc atgtgaccaa
      121 ctatctatat atcgttatat agtcctgtat atagcactat atgtagtgtc tggagaggga
      181 cgactaacac gatacagttt accctgaaaa gagacttcac agattcttta tatatttcaa
      241 taagttatgc taaaaagaga ctataccgat actgcataga atttgtgtag gattgcatag
      301 atacttggaa aaacaccgtt aaattgcata gtcattcacc tgatattgtc ctgtttttac
      361 aggtagttga ttcggatgtt ctaggaatca tcctcatagt tgattcggtt agttgggctt
      421 gtaccaaccc ctaatcaacc tcatagtgta tttagacctt gatgatttta gacagccgcc
      481 ctgttcatgc agcacgtcca cacagtgaag ctattcgcga cgctcaacga aaaaaaccta
      541 aagttccggt acatgctgta ctgacggcga caaatccgct gattcgtttt atcggtagtg
      601 atgatatgac gcaaaaccgg gaattatttc aggtctggtt acagaaatta gcgcagtggc
      661 atcagaccac tacgccttat ctttttttac atacgccaga cattgcccag gccccggaac
      721 tggtacatac cctgtgggaa gacttacgta aaacgcttcc agagatcgga gcagttccgg
      781 ctattccaca gcaatcttct cttttctgaa tttgccacct atcatagaca ggtgccatcg
      841 gccattttaa agggagtttg tatggtaagc gcgctatatg ccgttttaag tgcgttgtta
      901 ttaatgaagt tctcttttga tgtcgttcgc ctgcgaatgc agtaccgcgt tgcctatggt
      961 gacggtggtt ttagcgaact gcaaagcgct attcgcattc atggtaacgc ggtggaatat
     1021 attcccatcg cgattgtgct gatgctgttt atggaaatga atggcgcaga aacctggatg
     1081 gtgcatattt gcggcatcgt tttgcttgct ggtcgtctga tgcattatta cggttttcat
     1141 caccgtctgt ttcgctggcg acgttccggc atgagcgcca cctggtgtgc gctgttgctg
     1201 atggtgctgg cgaatctttg gtatatgccc tgggagttgg ttttctccct gcgttagcgc
     1261 acaatacgcc actttctttt tcccggattt ttacgttatg tctcaccgcg acacgctatt
     1321 ttctgcccct atcgccagac tgggcgactg gacctttgat gaacgggtag ctgaagtctt
     1381 cccggatatg atccagcgtt ccgttcccgg ctattccaat attatttcca tgattggtat
     1441 gttagccgag cgcttcgttc aacctggtac gcaggtttac gatctgggtt gttctctggg
     1501 cgcggcgacg ctctcggtgc gtcgcaacat tcatcatgat aattgcaaaa ttattgccat
     1561 cgacaactcc ccggcgatga ttgaacgctg cagtcgtcat attgacgcct ataaagcccc
     1621 tacgccagta gacgtcattg aaggtgatat tcgcgatatc gccattgaaa acgcatcgat
     1681 ggtggtgctg aattttaccc tgcaattcct ggaaccttcc gagcgccagg cattactgga
     1741 taaaatttat caaggactga acccgggcgg cgcgctggtg ctttcggaaa aattcagttt
     1801 cgaagatgcc aaagttggtg aactgttgtt caacatgcac cacgacttta aacgtgccaa
     1861 cggttacagc gaactggaga tcagccagaa gcgcagcatg ctggaaaacg tgatgctgac
     1921 cgattccgtg gaaacccata aagcacgcct gcataaagcc ggttttgagc atagcgagct
     1981 gtggttccag tgctttaact ttggttcact ggtggcatta aaagcagagg acgctgcatg
     2041 atcgactttg gtaactttta ttctctgatt gccaaaaatc atctttcaca ctggctcgaa
     2101 acgctgcccg cgcagattgc taactggcag cgcgagcagc agcacgggct gtttaagcag
     2161 tggtccaacg cggtggaatt tctgcctgaa attaaaccgt atcgtctgga tttattgcat
     2221 agcgtaaccg ccgaaagcga agagccactg agcaccgggc aaattaaacg cattgaaacg
     2281 cttatgcgga acctgatgcc atggcgcaaa gggccgttct cactgtatgg cgtcaacatc
     2341 gataccgaat ggcgttccga ctggaaatgg gatcgtgttc tgccccatct ttctgattta
     2401 accggacgca ccattctgga tgtcggctgc ggcagcggtt atcacatgtg gcgcatgatt
     2461 ggcgcaggcg cgcacctcgc ggtggggatc gaccctacgc aactgtttct gtgccagttt
     2521 gaagcggtgc gtaaactact gggtaacgat cagcgcgcac atttgttacc gttaggtatt
     2581 gaacagcttc cggcactgaa agcctttgat accgtctttt cgatgggcgt gctttaccat
     2641 cgccgttcgc cgctggagca tctctggcag ttgaaagatc aactggtgaa tgagggtgaa
     2701 ctggtgctgg aaacgctggt tattgatggc gatgagaaca cggtgctggt accaggcgat
     2761 cgttacgcgc aaatgcgtaa tgtctatttc atcccttccg cgctggcgct gaaaaactgg
     2821 ctgaagaagt gtggttttgt agatatccgc attgtggatg tgtgcgttac cactactgaa
     2881 gagcagcgac gcactgaatg gatggttacc gaatctctgt ccgatttcct cgacccacat
     2941 gatccgagta agacagttga aggttatcct gcgcctaaac gcgcggtgct gattgcgcgc
     3001 aagccgtaaa ggtctggtat tactgccgga tgcggcgtga atgccttatc cggcctacaa
     3061 acccttgcta attcaatcta ttgcagggac tatgtaggcc tgataagcat agcgcatcag
     3121 gcagttttac gtttgcataa cctcagcgcc cgtttccggg cgctattcac ttcttacgcc
     3181 tgaacagcag gcttatcaaa tgccgttaac ttcggtgcgt tacccgtata tttttcgata
     3241 tacaccagcg cagagttacc ggcacaggca tttgccagct gcgagctggg gatatccgcc
     3301 gttaacacgt tcgcactgcc gtttttacac aagccgtttt ccagatctgg ccatgcacct
     3361 tcatgcacgc ataccacgcc ttttttgatc ccgtcagtca ccaccgcgcc tgtcaaaatc
     3421 tgaccacgtt tgttccagac acgcaccaga tcgccattcg caataccaaa tcgagcagca
     3481 tcttcggtgt gaatagtgat cggttcacga tcggcaaccg catatttttt acgcagttcc
     3541 gcatagttaa gctgactgtg taaacggtgt gccggatgtg cggtcagaag ctgcaactgc
     3601 ttctcgtcgg cggtaccctt ccactcatca ggcgcaagcc aggttgggtg tgccgggcaa
     3661 tccttatagc caaatttttc cagcgttttg gagtaaatct caattttgcc acttggcgta
     3721 cccagcgcat ttttcaccgg atcggcgcgg aaatcaccat aacgaacgta ctgttcgttc
     3781 ttctcgctgc ggcgcatttc gatcagttta ttttgctgcc agaaggcatt aaacattggc
     3841 atagtgacgc gttgcgcacg ggcccctttc tgagcggcat catagaaaaa tttcagccac
     3901 gccatttcat ctttaccttc ggtatagatc tcttttccgc caggtttgag taattccgca
     3961 agatcggcaa acacatcaaa atcgtttcgc gcttcaaatt gcggagcaac agcctgcttc
     4021 atcggcacaa tatgctggtt gctgtaatca ccggtcatcg tcaagtcatt gcgctcaaac
     4081 gatgtggtga tcggtaatac gatatctgcg tgtttagcag ctgcggtcca gtagcattca
     4141 gaaacgacga tcatctccgg tttctgccat gctttaatca gacgattagt atcctggtga
     4201 tgggtaaagt taccgccacc cgcccaccag atcattttga tattcggata cgtttgctct
     4261 ttaccgttat gctgatattt gccgccagga ttttccagcg catcgacgat acgggcaaca
     4321 ggaatcgccc tcattccgcc atcatcagcg gcctcgctgg cctgtccggc aatcgccgca
     4381 gacatttccg gcagcacgcc gccaacacgt gtcgggttgc caccattgga gtaatgataa
     4441 gagaaaccaa aaccgccgcc cggtgtacca atctgcccta acatcgccgc cagcgtcacc
     4501 agcatccagt gtttctgttc accatattgc tggcgctgaa ttccccagcc tgccatcagc
     4561 attgtgcgat tcgccgccat cagttcagcg agtttgacta tctgcgcttc cggtacgcca
     4621 gtaatttccg ccgcccaggc cgcgctcttc ggcgtattat cgctcttacc tctcagatac
     4681 tcttcaaact gcggataccc ggtagtgtat ttttcaagga acactttatc gtgtttgcct
     4741 tgtgtcatca gggtatgcgc aatccctaac atcagtgcca cgtcggtgcc catattcggc
     4801 gcgatccagg tggcattatc gccaaagaag tcgatggttt cggagcggat aggatcaatg
     4861 gcaatcactg gtttgccaga ttttttcagc tgatggaagt attccagccc ttgctcatcg
     4921 gtactgctcc aggcaatttt taaggtattc agcgggttca tcccccacag caccacaacc
     4981 tggctgtttt ccagaatcag cggccaggag gtctgctgtt catacacctc tacagaaccg
     5041 accacatgcg gcatgatcac ctgtgccgcc ccggttgaat aatcgccgct atgcccggaa
     5101 taaccgcccg ccaggttcat ataacgttgc agtaaggttt gcgctttatg caacacgcca
     5161 gaagagcgcc agccgtatga tccggcaaaa atagccgatg gtccgttagc tttgcgaatg
     5221 cgatcgtgtt gctcatgaat cagttttaat gcttgttccc agctcacctg tacataggta
     5281 tcttcgccac gacctttcgt cggttgcagt ggattatcga gatagctttt tctcaccatc
     5341 ggatgctgaa tacgcgtcgt ggtgtgtacc tgatccgccg ccgtagactg taaggaattc
     5401 ggtatggttt tcgccagcgc gcctgttgaa gaaacaatct tgccgtcctt cacttctacg
     5461 ttcatcgctc cccaacgtcc cgcggtgagg attttaccgt ccttctcttc tgcccatgcg
     5521 gggagcggtg ctgccgatgt caccaccagc gctccagcgg caataccgct gtgtttaata
     5581 aattcacgtc ttgttaatgt cataacttcc tccctgatca acgaggatca ctgtttctcg
     5641 gtaatatctt tggcgttgta ctggaaatac cgcgttaaaa tgtccagttc gttttcgctc
     5701 atgctggttc gtgcccccat tcctttggca atggacggcc acgcattgac ggtgtaatgg
     5761 tcggcggcaa taggggcatg acaaccagcg caataggtat cgtcaagttt ttcagcgtat
     5821 tgccatagcg gtttacggtc cgctaatccg ggatcggtaa gcgcaccctg taaagacgcc
     5881 tgacgccatt gattgccgta ttcgtcagcc tgccattccc cgtttaccgt gagcgccttg
     5941 ataccttctt cacttaatgt ggctagcgcc agccgttgac ctgccgccag gtagagcgtg
     6001 ttttcactgc cctgcatttg ataaccctgc aacagaacga tcggctgttt gccactggca
     6061 tcaacgacgg tgagatcggt accaggattc acggtagcca gctcgcctat gcgagaagtt
     6121 ttgaaaggat aaatatgtgc gccattagtc actgaagtag cggcctgact ttccagctca
     6181 tgcgccgcgt tgtcatccat ttttatttct ggcggaaaat gggcaatgcc tttatgacaa
     6241 tcgatacagg tttcgccgcc cttttgtgct ttgttatgca ttttctgcgc agattcactt
     6301 tgcgaggcaa tatccatggc atcaaaagaa tggcaactac ggcacgttgc agagtcagtt
     6361 gcttttaatt ctttccatac tgtttcggcc atttcctggc gatgagtttc gaacttatcg
     6421 tcactgtcta ttttgccgct aacaaattca tgataaatat ctttagatgc ttttaattta
     6481 gcaaataaat aatccatccc tgactttgga atatggcaat ctgcacattc cgcacgtatc
     6541 cctttctggt tcgaaaagtg gacagttccc tgatattcct caaaaggttt actcatcgag
     6601 tggcaggaaa ggcaaaatgc tgtatccgac gttttatgta aggctttttg cgccagcaat
     6661 aacccgccgc caccaaccac aaccgctatc agcaaaaaca ataacccaat gcgttttttc
     6721 cctcgcatag attcctctaa ataaattcca gaacaccgaa atcattgtat aattaaatac
     6781 acaacgaatt aaccgcttgc ataactcggc acaacactgc ctgaaataat cgataaagaa
     6841 tatgatttat tacaatgtaa tcattaattg ctaa
//
LOCUS       DAZOZY010000203         6826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZOZY010000203 DAZOZY010000000
VERSION     DAZOZY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.204"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHURQ_003587"
     CDS             complement(155..667)
                     /locus_tag="ACHURQ_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR0825277.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHURQ_003588"
     CDS             862..1626
                     /locus_tag="ACHURQ_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825278.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHURQ_003589"
     CDS             1577..2980
                     /locus_tag="ACHURQ_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR0825279.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHURQ_003590"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHURQ_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHURQ_003591"
     CDS             4337..5080
                     /locus_tag="ACHURQ_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR0825280.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHURQ_003592"
     CDS             5084..6304
                     /locus_tag="ACHURQ_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR0825281.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHURQ_003593"
     CDS             6308..6814
                     /locus_tag="ACHURQ_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825282.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1816 a         1622 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacgaa
//
LOCUS       DAZOZY010000204         6761 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZOZY010000204 DAZOZY010000000
VERSION     DAZOZY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.205"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..2606
                     /gene="cas3"
                     /locus_tag="ACHURQ_003594"
     CDS             <1..2606
                     /gene="cas3"
                     /locus_tag="ACHURQ_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR0825283.1"
                     /translation="DDYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAA
                     QFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSEC
                     QELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLEIK
                     PLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYW
                     QKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFIL
                     EDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESR
                     PSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGV
                     GTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSV
                     ILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKD
                     VERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSL
                     SLFHSRFAFSDRQRIETETLARFGKEDSSQRAGKVLICTQVLEQSVDCDLDEMISDLA
                     PVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRN
                     SAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGK
                     YYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATG
                     AHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGY
                     SATEGLIGKVG"
     gene            2704..4266
                     /gene="casA"
                     /locus_tag="ACHURQ_003595"
                     /gene_synonym="cse1"
     CDS             2704..4266
                     /gene="casA"
                     /locus_tag="ACHURQ_003595"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR0825284.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4263..4799
                     /gene="casB"
                     /locus_tag="ACHURQ_003596"
                     /gene_synonym="cse2"
     CDS             4263..4799
                     /gene="casB"
                     /locus_tag="ACHURQ_003596"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR0825285.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            4811..5866
                     /gene="cas7e"
                     /locus_tag="ACHURQ_003597"
     CDS             4811..5866
                     /gene="cas7e"
                     /locus_tag="ACHURQ_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR0825286.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5877..6623
                     /gene="cas5e"
                     /locus_tag="ACHURQ_003598"
     CDS             5877..6623
                     /gene="cas5e"
                     /locus_tag="ACHURQ_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR0825287.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6548..>6761
                     /gene="cas6e"
                     /locus_tag="ACHURQ_003599"
     CDS             6548..>6761
                     /gene="cas6e"
                     /locus_tag="ACHURQ_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR0825288.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
BASE COUNT         1678 a         1611 c         1839 g         1633 t
ORIGIN      
        1 gcgacgacta tcaccttctt tgctggcatt cgctggatgt agccgcgatg ggctatttaa
       61 tggttaaaag aaattgcttc gggctggctg attattttcg tcaattaggg atttctgaca
      121 aggaacaggc ggctcaattt ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg
      181 cccgctcttt tcaacaactt tacctgcccc ctgaactcaa gattcaggaa ggtgccagaa
      241 aaaattacga aaaaatctct cattcaacgc tgggctactg gttgtggaat cattatttaa
      301 gtgaatgtca ggagttactt ccttcatctt cgctctctcc gcgcaaactt aggcgagtga
      361 tagaaatgtg gatgcccgtg acgacagggc atcatggtcg gccccctgat cgtatggatg
      421 aattggataa ttttctgcct gaagacaaag ctgcagcacg agattttctc cttgaaatca
      481 aaccactgtt tccgctcata gagatccccg cattctggga tgatgatgag ggcattgaac
      541 ttataaaaca cctttcctgg tatatctctg caaccgtcgt gctcgcagac tggacgggtt
      601 cgtcaacgcg attttttcca cgcgtcgcac acccaatgga tattaaaggt tactggcaga
      661 aaactttagt tcaggctcaa aacgccttaa ccgtctttcc tctaaaagca aaagtcgcgc
      721 ctttcaacgg aattaatacg ctgtttcctt ttattgagaa tccgacaccg ttacagcaaa
      781 aggtgctgga tctggatatc agccagcaag gaccgcagtt atttattctg gaagatgtga
      841 ctggcgcagg taaaacagaa gcggcgctta tcctggcgca caggttgata gctgcgggga
      901 aggctcaggg gctgtttttt ggtctgccaa caatggcaac ggccaatgcc atgtacgatc
      961 ggctggtcaa aacctggctt gctttctatt cgccagaatc ccgccccagc ctggtgctgg
     1021 cacatagtgc ccgcacatta atggaccgct ttaatgaatc actctggtct ggtgatttag
     1081 tcgggtcaga agaaccggat gaacaaacgt tcagtcaggg atgtgcggcc tggtttgccg
     1141 acagtaacaa gaaggcgttg ctggctgaaa ttggcgtcgg cacgctggat caggcgatga
     1201 tggcggtgat gccgttcaaa cataataatc tgcggcttct ggggctgagt aacaaaatcc
     1261 tgctggctga tgagatccac gcctgtgacg cttacatgtc gtgcattctt gaagggttga
     1321 tcgagcggca ggcgcgtggc ggaaacagcg ttattttgct ttctgccacg ttatctcaac
     1381 agcagcgcga caaactcgtc gctgcctttg cgcggggtac agagggcctg caagaagctc
     1441 cgttcctgga aaaggatgat tacccctggc tgacgcatgt cacgaaatcc gatgtgaact
     1501 cacaccgggt agcgacgcgc aaagacgttg agcgtagcgt cagcgtgggt tggcttcata
     1561 gtgaacaaga gtgtattgcg cgtatcgaat cggcggtaag tcaggggaaa tgcatcgcct
     1621 ggatccggaa ttctgtcgat gacgctatta aggttcatcg tcagctgctt gcccgcggcg
     1681 tcattcctgc ttccagcctt tcactttttc atagccgctt tgcttttagc gatcgccagc
     1741 gaattgaaac ggagacactg gcacgctttg gtaaagaaga tagctcacag cgtgccggaa
     1801 aagtccttat ttgtactcag gtattagagc agagtgtcga ttgtgacctg gacgaaatga
     1861 tctccgacct ggcccctgtt gatttgctga ttcagcgagc aggacgatta cagcggcata
     1921 tccgcgatat taatggtcag ttaaagcgtg acggaaagga cgagcgttcc ccccctgaat
     1981 tgctgattct ggctcccgtc tgggacgact ctcctggcga cgaatggttt ggcagtgcca
     2041 tgcgtaacag tgcatttgtc tatcccgatc atgggcgaat ctggctgacg cagcgtgtgc
     2101 tgcgtgagca aggtgctatt cagatgccac acgcagcccg gcttcttatt gaatccgtct
     2161 acggtgagga cgtggtaatg ccggaaggat ttgcccgcag cgagcaggag caagtgggca
     2221 aatattactg tgatcgcgca atggctaaaa agtttgtgct gaacttcagg cccggctatg
     2281 ccgccaatat caacgattac ctcccagaaa aactgtcgac acgtctggct gaggagtctg
     2341 tttccctgtg gctggctacc tgtattgacg gtgtggtgaa gccttatgcc accggtgctc
     2401 acgcatggga aatgagcgtt gtcagagtgc gccgaagctg gtggaaaaaa catcgggatg
     2461 agttttcttt actggaggga gaggcgttca ggctgtggtg cattgaacag cgacaagatc
     2521 cggagatggc aaacgttatt ttagtcaacg atgacgaaag ttgcgggtat tcggctacgg
     2581 aaggattgat tggcaaggtt ggttgagatg tctgcgacag gactctctga atagttgata
     2641 caccgaaaag gatggaaaga tggacaaccg acctgggatg gtctttttta cgagaggatg
     2701 cgtatgaact cgttttcact tctgacaacc ccgtggttgc ccgttcgttt taaagacgga
     2761 acgacaggca aactggcgcc agtcgatctg gcggatgaaa atgttgtcga tatttctgcg
     2821 ccgcgggcag atctccaggg tgcggtatgg cagtttttgc tggggctact acaaaccagc
     2881 ttcgcgccaa aagatcatcg tcgttgggat gatatctggg aagacggact ggaagctgaa
     2941 aagctacggg aagcattgct gtcattagaa catgctttcc agtttgggcc agattcccct
     3001 tcatttatac aggatttcga ggcgctcacc ggagataaag ttccggtcgc ttcgctactg
     3061 cctgagattc ccggcgctca aacaacgaag tttaataaag accactttat caagcgtggt
     3121 gtaacggaat acctgtgccc acattgctct gcgttagctc tgttctccct acagttgaat
     3181 gcgccgtcag gtggcaaagg ctaccgcacc ggtttacgcg gcggtgggcc gatgaccact
     3241 ctgattgaat tacaggaata tcagggtaat caacaaactc ccttgtggcg caaactgtgg
     3301 atcaatgtca tgccgcagga tgaagccgat ttaccgctac ccaaaaaatt tgacgatctg
     3361 gttttcccct ggcttggccc gacgcgcatc agcgaactgg ccggtgcggt ggtaaccgat
     3421 gagcaggtca ataaactcca ggcgtactgg ggaatgcctc gccgtattcg tattgatttt
     3481 aataccacca cagtcggcaa ctgcgatatt tgcggtgagc agagcgacgc gcttctgagc
     3541 ctgatgacga ccaaaaatta cggcgctaac tatgccatgt ggcagcatcc cttaacgcct
     3601 taccgtgtac tacttaaaga gggcggcgag ttttactcgg tcaaaccaca accgggcggt
     3661 ttaatctggc gcgactggtt aggccttatc gaaacgggta agtcagaaaa caatacggaa
     3721 cttcccgcgc tggtggtgaa actctttaat gccagcagtc tcaagcaggc aaaagtgggc
     3781 ctgtggggat ttggctatga tttcgacaac atgaaagcgc gctgttggta cgaacatcat
     3841 ttcccgctgc tgctcaataa aaaagaaggc cagataccga aactgcggct ggctgcgcaa
     3901 acggcttcac ggattctaag tctgttacgg agtgcattga aagaagcatg gttctccgat
     3961 ccaaaaggtg caaggggtga ttttagtttt gtggatatcg acttctggaa caaaactcag
     4021 catcgcttcc tgaggttagt gcgccaaatt gaagaaggtc aggatgcgga tgaattactc
     4081 agcaaatgga ataaggaaat ttggttattc gcgcgtcagg attttgacga gcgtgtattc
     4141 accaatcctt atgagcccgt tgatttggaa cgcatcatga ccgcgcgcaa gaaatatttt
     4201 acaacatcgg cggagaagca aagtgctaaa gccgccaggg agaaaaagca ggaggctgct
     4261 gaatgagtat tgtaaaagag gagcacaaag ccacgctgcg caaatggcac gaggaattgc
     4321 aggagaaacg cggcgaacgt gccagtctgc ggcgtagtac gacggttaat gatgtttgtc
     4381 tgacggatgg atttcgcctg tttctgaaga acagacaaat aaagtggcag gatgaacctg
     4441 agtggcgtat caccgcactg gcgctgatag ccgcagttag cgccaatgtt aaagccattg
     4501 atgaacggca gccttttgcc gcgcaactgg cggcggtaat gtcaaagggg cgttttactc
     4561 gcctgtcagc agtaaaaaca ccggatgaat tactgcgcca gttacgccgc gccgtcagat
     4621 tattaaacgg ttccgtcaat cttgactccc tggcagatgg cattttccgc tggtgccagg
     4681 agagcgacga cctgctcaat catcaccgcc gccagcagcg gccaaccgaa tttatccgta
     4741 tccgctgggc gctggaatat taccaggctg gcgacgccga taacgaacaa aatcaataaa
     4801 ggactatctc atgacaacat ttattcagct tcatgtgtta accgcttacc ctgctgccaa
     4861 ccttaaccgt gatgacaccg gtgcgccaaa aactgtggtg ctgggtggag caacgcgtct
     4921 gcgcgtttcc tcgcaaagtc tgaaacgtgc gtggcgcact tctgcacttt ttgaacaggc
     4981 actggcgggc catattggta ttcgcagtgg gcgtattgcg cgtgaggcgg caactatcct
     5041 gattgagaaa gggattgaag ataaaaaagc catcgaatgg gcggcaaaaa ttgctgatta
     5101 tcttgggaaa gctaaaaatg acaaaaaacc aaaagatccg ctcactaacg ccgaaactga
     5161 acaattagtt catatcagcc cggcagaatt tgacgccgta aaagcgcttg ctcatcaact
     5221 ggcagaagaa aagcgcgcgc caaaagagga agatctcgcg ttgttacgta aagaccgtat
     5281 agcggtagat atcgctatgt ttggtcgtat gctggcgaat aaacctgagt ttaatgttga
     5341 agccgcctgc caggtcgcgc atgcgtttgg tgtcagtgaa acgattgtcg aagatgattt
     5401 tttcaccgcc gttgatgatt tgcgccaggc ctctgaagat gccggtgccg ggcatctcgg
     5461 agaaaccgga ttcggttcag cacttttcta cacctatatc tgcattgata aagatctgct
     5521 ggtagaaaac ctcggcggtg acgaagcgtt agctaatcag accatacgcg cttttacgga
     5581 agccgcgctt aaagtttccc caaccggtaa acaaaatagc tttgccagcc gtgcctatgc
     5641 ttcctgggcg ctggcagaaa aaggcaccga tcaaccacgc tctctggcgg cggctttcta
     5701 tgaacccatt aatggcacac gtcagttaga tgtggcggtg cagcgtatta caacgcttcg
     5761 cgaaaacatg aatacggtct acgaacagaa gaccgaatgc gcaagttttg acgtgatgaa
     5821 caaacaggga agcatgaagg acgtgctgga ctttatctgc gcgtaagggg cggcttatga
     5881 gccaatatct gatttttcag cttcatgggc cgatggcgtc ctggggtgtc gatgctccag
     5941 gtgaagttcg tcatacccat gaacttcctt cgcgctcagc attgctgggg ctactggctg
     6001 ccggggtagg gattcggcgt gatgataccg aacgcttaaa cgcgtttaat cgccactatt
     6061 cactggtggt ttgcgccagc cgtaacccgc gctgggcacg ggattatcac acggtccaga
     6121 tgccaaaaga ggtgcgtaaa gcacgttatt tcagccgtcg tgaagagttg agcgaccctg
     6181 agcttttgag cgcgattatc tcccggcgcg actactacac cgatgcctgg tggatggtgg
     6241 cggtggcaac aacagccgat gcgccttaca gcctcgaaca gttgcaggac ggtttacgcc
     6301 atccagtttt tccgctttat ctggggcgaa aaagtcatcc gctggcatta cctcttgcgc
     6361 cgttactgct cgaaggcaac gcgtgtgatg ccttatgtaa tgcataccaa cagtatcagg
     6421 atcatttcca taaactgaaa gtctcactcc cgaaacttca ggatgaatgc tggtgggaag
     6481 ggaaacacga cggcctggtc gcgagcaaaa tattacgccg ccgggatgtt cctttaaatc
     6541 gtcagcagtg gctgtttggg gaacgcaccg tcaatcaggg gccgtggctc agcaaggagg
     6601 aaccatgtac ctctcaagaa taaccttaca taccggccag ctttcgcctg cgcagttgct
     6661 gcatctggtg gatcgcggtg aatatgtgat gcatcagtgg ctgtgggatc ttttccctgg
     6721 cggcaaagaa aggcaatttc tttatcgtcg ggaagaactc c
//
LOCUS       DAZOZY010000205         6731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZOZY010000205 DAZOZY010000000
VERSION     DAZOZY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.206"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1096)
                     /locus_tag="ACHURQ_003600"
     CDS             complement(<1..1096)
                     /locus_tag="ACHURQ_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0825289.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNM"
     gene            complement(1133..2578)
                     /gene="abgB"
                     /locus_tag="ACHURQ_003601"
     CDS             complement(1133..2578)
                     /gene="abgB"
                     /locus_tag="ACHURQ_003601"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR0825290.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            complement(2578..3888)
                     /gene="abgA"
                     /locus_tag="ACHURQ_003602"
     CDS             complement(2578..3888)
                     /gene="abgA"
                     /locus_tag="ACHURQ_003602"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR0825291.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            4064..4972
                     /locus_tag="ACHURQ_003603"
     CDS             4064..4972
                     /locus_tag="ACHURQ_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0825292.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            5302..5865
                     /gene="smrA"
                     /locus_tag="ACHURQ_003604"
     CDS             5302..5865
                     /gene="smrA"
                     /locus_tag="ACHURQ_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR0825293.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQVKQENWERHAKRSR"
     gene            complement(5886..>6731)
                     /gene="dgcM"
                     /locus_tag="ACHURQ_003605"
     CDS             complement(5886..>6731)
                     /gene="dgcM"
                     /locus_tag="ACHURQ_003605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR0825294.1"
                     /translation="FYARFFLTNSAPMLLIDPSRDGQIVDANLAALNFYGYNHETMCQ
                     KHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQTYAGPIEIYGDKL
                     MLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQMQHLAIAQDYSLLL
                     IDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGEEFVLLLPRTPLDT
                     ALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRVDDALYRAKNDGRN
                     RVLAA"
BASE COUNT         1684 a         1717 c         1704 g         1626 t
ORIGIN      
        1 ccatgttgct ccagttaaac atggcgacaa actgggcgag gggaaaaacc atcacgataa
       61 atcctgccat ctctttcatc ggttcaatca ttaactgcgg taaatccgcc tgacgtcgaa
      121 ttgtgcgggt agcgatgcca taagccagcg agacaacaaa gaaaaaaaga atgatcagtg
      181 gcacgatacc tttaataaag ggtgatggca tcacggtgtg attaatcgga tcgcgcaata
      241 tcccgttttc cgggatcacc atcagcgcaa tcgcagcaat aaaaagtagc gatacgacac
      301 ctgctatgcg taaaccaaaa cgctgacttt cggtcaatgt ctgcagtttc tcatcgctgt
      361 ttccctgcca ttgacctaac cgtggctcga tgattttgtc ggttatcagg ccgccaacaa
      421 tcgtcagtac gactacggag ctggccataa aataccagtt atcaattaca ctgacgtgca
      481 tttgcggatt gaacgcagcc gccgcttccg tgctgatccc cgacagcaac acgtcggttg
      541 tgacaatcag taaattagcc gtaaagccgc aacctacgcc tgcaatggca gccagtaaac
      601 ctgcaactgg atgcctgccc accgccagaa aaatcagcgc acccatcggt ggcatgatca
      661 ctaacgtcgc atcggaagaa atgtggctga aaaaagcaat aaacagcacc atataactgg
      721 cgtagcgggc attaacatgc gatgccattt taaccattag cgctggcagt aagccgacgc
      781 gctccgccag accggcacct aaaaccagcg ccaggatcgc accaagtgga gcaaaaccgc
      841 taaagttttt aataacattg ggtaaagacc agtgtaatcc ttccacactg agcaggtttt
      901 tcaccaccac cggcgttcca tcggtcgggt ttttcgcact gacgccaaag gccgacaaaa
      961 ttgccgtcgt caccatgagt acgataatca aatagataaa gagcaaaaaa ggatggagaa
     1021 ccttgttacc aattctttcg acccagccat agagcttccc ggattgggag gacgacggta
     1081 tggatgacat actcatgggc attcctcggt tgttgtgttt gcgttgttgt tgttatttta
     1141 aaggtgacgg tgtcacgttt ttcgggatag ggcagtgata cggttgcgtg tccgttactt
     1201 gctgatgctc ttgttggcat tcttgcaata gccctgaatc aatgaagaga ttgactgtgg
     1261 ttgctgccat agttttcgcc gccagcagca ttcctttatg agcaatagat gttcgcccct
     1321 ggctaaccag ttgccacgta tgtagcggtg taccgacggc aaaacagggg ctgaaacact
     1381 gggcaacagg cagtttccaa ctaacgtcgc cgacatcagt cgatgccgca agcacgttat
     1441 cggtggcggc atatggagcg acctcattcg ccagtaccgt ttcacgatga cgtagtgcaa
     1501 aagccttgcc gttttcgcca ccggttgcgg cgatattatt cagactgttt tgccgatcgt
     1561 tgggggtgag cgtagcctga atttgtttcg caaaagccag ttcttcggag ttccattccg
     1621 gggtaccaaa atgggatagg gcatggtaca tggcattttc taaggtgcga ttcgggagat
     1681 aactggaaca ggctttgtcg aagcggcatt caaccgtggt ttcggtcatc aatgccgcac
     1741 cttcggcgat tttggcgacc cgatcataaa tatgctgcac gtcggtcatt tcgggggcgc
     1801 ggataagata aagcacttct gcctgcgcct ggaccacgtt gggcgagatc ccgccgctat
     1861 ttgtgatggc atagtgtacg cgcgcttttt caataatatg ttcgttgagg aagttggtgc
     1921 cagtggtcat caacgttacg gcatcaaggg cgctgcgtcc caaatgaggg gaattcgcgg
     1981 catgtgctgc gatcccttta aagcgccatg atgcctgaat gtttgccagc gtgcgggtat
     2041 tgaacatacc ggcaaaggct tccgggtgcc aggtgagtgc cgcatccaca tcatcaaata
     2101 ccccctcgcg gaccatgaac gttttacccg agccgccttc ttcgccagga caaccataaa
     2161 agcgcactgt gccgccttgc ccatattgtt ccagccattt cttgacggct attgcagcgg
     2221 caaaggcggc ggttccccgc aaattgtgtc cgcaaccgtg accattttca ccgggcgtcg
     2281 cggatgtagg ttgcgcgcaa ccagcttgct gacttaaacc tgccagggcg tcatattctc
     2341 ccagcagggc gataaccggt ttgccttgac caaacgaagc aataaaggca tttgggatat
     2401 tgcctacgtt gcgggtaacg gtgaagcctg cagattccag agccgaagcc agatgctccg
     2461 ctgaccagaa ctcttcaaaa cgtgtttctg gatgatccca gatttgatcg gcaatatcgg
     2521 tataacgttg gcgatcggct tcaatcgcat cgtcgataaa acgatagatt tcctgcatca
     2581 gatacctcgc gtccagggaa aattgagcgc ggtgcgcgcc agcgtttcga cggcaatagc
     2641 gagaacctgc tcgtcaaaat cgaatttttc gttgtgatga cctgccgcca gctgtgtgcc
     2701 aaacaccacg taggaggctt gcccttgatg ttgctgcacg cgggccatca ttaatgtggc
     2761 atcttcggaa cccgcaggcg cttcaacacg ttcaatggcc tgattgaccc ccgcgacctg
     2821 agccgcctga ctttgcaacc atgcgaccca ttgcggcgaa ggagaactgg cggtagctgc
     2881 acccatcaga cgagtttcaa cgccgacacc atacatggtt gctgcgccct gaatcgcttg
     2941 ttgtgcacgg tcaaaaacat attgattaat gacgtcgctg gccccgcgtg tttccacttt
     3001 cagcaacgcc gaggcaggaa caacgttacg accgcttcct gcctgcataa cgcccacgtt
     3061 tactctggaa gctccttcgc tgtgcggggc gattgcatgc agtgcaagag tggcttgtgc
     3121 tgccgccaac aaggcattgt gaccgtcttc tggttttgcg cctgcgtgag cggcggtacc
     3181 ggtgaagtgc gcgtcaaatt tggtggttgc cataaaatta tcactgccgc acaccacggt
     3241 gcccgcaggt acgccagtgc caatgtgcac ggcagtaaaa taatcaacat catctacgac
     3301 acctgcatcg accatcgccc gcgcgccacg cgtaccttcc tctgcaggct gaaaaatcag
     3361 tttgatgacg ccatgtagtc cggactcgaa ttgtttaagg gtatgcgcca gcccaagccc
     3421 aatggcggta tgtccatcat gaccacaggc atgcatcatt ccggcgttac atgacgcaaa
     3481 accgtcgcgg taggggcgat ggctgacatc ctgctcttca ctgagatcca gcgcgtccat
     3541 atcgacacgg aaagccatca ccggaccggg gcgaccggta tccagggtgg cgacgatacc
     3601 agtgaaacca ccttcaaaaa ccgcaatcca ttgtgctagc gcaccctgtt gacgagcgcg
     3661 ctcgaattcg cgttgtagag tgagttcatc aggtagtccc atccggctac tttcattaac
     3721 tacttcgcgg cccagcgcca gtgaatagcc gagctgctgc aattcttccg caacaagggt
     3781 ggcagtgcgg aattccaccc agccagactc tgcatagtga tgaaaatcac gtcgccagtg
     3841 cgataatttt ggggcaagcg aattaacaaa ttgattcaaa gactccataa cctttcccgt
     3901 catcagtaaa aagtgtgacc cggttcacgt agcgatagtt tttacttatc actaactgat
     3961 ttttcacagt tttaaccgtt cataaattac cctgacacaa tcatctgcat taaagtagat
     4021 gccagtttct ttggtctgat aaataacggt tatcggtggc gtcatggctt ttcaggtaaa
     4081 aattcatcaa attcgggctt ttgttgaagt ggctcgtcag ggcagcattc gcggagcgag
     4141 ccgaatgttg aatatgtcgc aaccggcact gagtaaatct attcaggagc tggaagaagg
     4201 attagcggcg caactctttt ttcgccgtag taaaggcgtg acgttaactg atgccggtga
     4261 aagtttttat cagcacgcca gtcttattct tgaagagctg cgcgcagccc aagaggatat
     4321 tcgccaacga caagggcaac tggcagggca gattaatatc ggcatggggg ccagtatttc
     4381 ccgcagtctg atgccagctg tcatatctcg ttttcatcag cagcatccgc aggtaaaagt
     4441 acgcattatg gaagggcaac tggtgtcgat gattaatgaa ttgcgtcagg gagaattgga
     4501 tttcaccatc aatacctatt atcagggacc gtacgaccac gaatttactt ttgagaaatt
     4561 actggaaaag caattcgcga tcttttgccg cccgggacac cccgccattg gtgcccgttc
     4621 gatcaaacag ttactggatt acagctggac aatgccgacg ccacacggca gttactacaa
     4681 acagttgagt gaattgcttg acgatcaggc gcaaacgcca caggtcggtg tagtctgcga
     4741 gacgttctca gcctgtatca gtctggtggc aaaaagcgat tttctcagca tactgcctga
     4801 agaaatgggc tgcgatccct tgcacggaca ggggctggtg atgttgccgg ttagcgaaat
     4861 tttaccgaaa gcggcctatt atttgattca gcggcgtgat agtcgccaga caccactgac
     4921 cgcgtcatta atcacgcaat tccggcgaga atgcggctat ctgcaaagtt aaaactgcat
     4981 aaaaaaatag agtctgtcga catccgccag actctacagt acacacagca gtgcatccgc
     5041 gtcttaaatc cggcattgtc tcctctgcgc cggtgactgt gttataacgg ttttaacctt
     5101 tagttgccaa ttttcttcag tgacagattt cacgaaaata taagttacat tattaatatc
     5161 gtgaatgaat aatcatgcat aagcattttg cttaaaatat cggcaatatt tggaacttat
     5221 tactggaatt ttgggtaata cgttgttgga ccgacccggt ctggttatca tatcgcgctc
     5281 ttaattgcgg gaggatgtaa catgaacctt gacgacaaat cgctgtttct tgacgccatg
     5341 gaagatgtcc agccgctgaa acgtgctacc gatgtccact ggcatccaac gcgtaaccaa
     5401 cgtgcgccgc agcgtatcga cacgctgcag cttgataatt tcctcaccac cggatttctc
     5461 gacatcatcc cactaagtca gccgctggag tttcggcggg aagggttgca acatggggtg
     5521 ctggataagc tgcgcagtgg taaatatccg caacaggcga gcctgaatct tttgcgccag
     5581 ccggtggaag agtgccgcaa aatgatgttc agtttcattc aacaagccat ggctgatggt
     5641 ttgcgtaacg tgctgattat tcatggcaaa ggacgggatg ataaatcgca tgccaatatt
     5701 gtccgcagct atgtggcgcg ctggctgacc gaatttgatg atgttcaggc atattgcact
     5761 gcgctaccgc atcatggcgg cagcggggcg tgttacgtcg cactacgtaa aacggcgcag
     5821 gtgaagcaag aaaactggga gcgccacgct aagcgcagtc gttgatctcg agacgcatcc
     5881 gcggcttatg ccgccagcac gcggttgcgt ccatcatttt tcgcccgata caaagcatca
     5941 tcaacgcgtt taaacagttc atcgatgctt tcatttcctt cgtgatgcgc cacaccaatg
     6001 ctgacggtaa agcgtggtaa gcccgaaata ctcacttttg ccacgcttac gcggatagtt
     6061 tcagccagcg aaagcgcggt atccagtggg gttcttggta gcaataagac aaactcttcg
     6121 cctccccaac gaaacaccaa atcgcctttg cgagcgcaac tttcgagggt gcgggcgagg
     6181 gcgcataaca cctcatcacc tttagaatgc ccatagagat cgttaatgtg tttaaaacga
     6241 tcggtgtcga tgagcaacaa gctgtaatct tgggcgatgg cgagatgctg catttggcct
     6301 ggctcggtaa tgtgataaaa ctgtcgccga ttcagtaatc cggtcatcgc gtcatggtgg
     6361 gcagcatgtt ccagctgctc ctccagccgt ttttgctcag taatatcatg tacaatacat
     6421 aacatgagct tgtcgccata aatttcaatc ggcccggcgt aggtctgcac atgacgagtc
     6481 gaaccatccg ccagcttatg aataaaattc agaggtttat gaccaccggg taaatgcgaa
     6541 atttcatgca tgataggcat gacgcgacgc ccgagcatat ttatttccca ggtatgtttc
     6601 tggcacatcg tttcatggtt ataaccatag aaattgagcg cggcgaggtt agcatcgacg
     6661 atttggccat ctcgtgaggg gtcaatcaac aacattggtg cagagttagt cagaaaaaag
     6721 cgcgcataaa a
//
LOCUS       DAZOZY010000206         6730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZOZY010000206 DAZOZY010000000
VERSION     DAZOZY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.207"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHURQ_003606"
     CDS             38..250
                     /locus_tag="ACHURQ_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825295.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHURQ_003607"
     CDS             283..501
                     /locus_tag="ACHURQ_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR0825296.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHURQ_003608"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHURQ_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHURQ_003609"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHURQ_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR0825297.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHURQ_003610"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHURQ_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHURQ_003611"
     CDS             1917..2660
                     /locus_tag="ACHURQ_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR0825298.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHURQ_003612"
     CDS             2690..3229
                     /locus_tag="ACHURQ_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR0825299.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHURQ_003613"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHURQ_003613"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR0825300.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHURQ_003614"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHURQ_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR0825301.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHURQ_003615"
     CDS             4582..6102
                     /locus_tag="ACHURQ_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0825302.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6730)
                     /locus_tag="ACHURQ_003616"
     CDS             complement(6135..>6730)
                     /locus_tag="ACHURQ_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR0825303.1"
                     /translation="RAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQKQ
                     IITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATGK
                     TATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVK
                     NGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1774 a         1538 c         1589 g         1829 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaatggtat gctctttact cgctttaaac
     6541 tgagtaatga tactatcagt gatagctatt ccatctctac aaacaatagt gatgatttgc
     6601 ttctggtctc gccattgttc aaccgtagaa ataaaggttt ctacaagttg cccctgtctg
     6661 gttaatagaa ctgaacccgt cgaatcaacc actacaacac cgctaataga gatggtctta
     6721 ggtgctctct
//
LOCUS       DAZOZY010000207         6608 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZOZY010000207 DAZOZY010000000
VERSION     DAZOZY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6608
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.208"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..541
                     /locus_tag="ACHURQ_003617"
     CDS             <1..541
                     /locus_tag="ACHURQ_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR0825304.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
     gene            complement(597..1139)
                     /locus_tag="ACHURQ_003618"
     CDS             complement(597..1139)
                     /locus_tag="ACHURQ_003618"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0825305.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1123..1434)
                     /locus_tag="ACHURQ_003619"
     CDS             complement(1123..1434)
                     /locus_tag="ACHURQ_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR0825306.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHURQ_003620"
     CDS             complement(1619..2509)
                     /gene="cyoE"
                     /locus_tag="ACHURQ_003620"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR0825307.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2521..2850)
                     /locus_tag="ACHURQ_003621"
     CDS             complement(2521..2850)
                     /locus_tag="ACHURQ_003621"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR0825308.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(2850..3464)
                     /locus_tag="ACHURQ_003622"
     CDS             complement(2850..3464)
                     /locus_tag="ACHURQ_003622"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR0825309.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHURQ_003623"
     CDS             complement(3454..5445)
                     /gene="cyoB"
                     /locus_tag="ACHURQ_003623"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR0825310.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHURQ_003624"
     CDS             complement(5467..6414)
                     /gene="cyoA"
                     /locus_tag="ACHURQ_003624"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR0825311.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1788 a         1683 c         1634 g         1503 t
ORIGIN      
        1 gggcattatg tacgccaatg gtcagtatgt tgaggtagat tgtaagaaag cgaaggaata
       61 tcttgataaa ggtgtccaca tttatggtgg cccggaagac tttctggcta cttgtcgaaa
      121 agacatgatt gacagaaaaa cggttgacga tacgttaccc gtgattacgg ttacccgttc
      181 tgggatgcga gataattttt tagataaagg tttttcctgc atggatagcc tcttcgccac
      241 caccaataaa ttaggcgaag tggccaatct tcgcgtgaca ttcagcattc gtcgcccatc
      301 cggtaaggag attaaccaaa cggttggctt cgcgcctttc ggtttaaacc ggctgaatat
      361 tagctttact gattacctat ttggttcatt taccagtaat tcgtctctta ttctttataa
      421 accggaattt gagcgaaaat cttgtgcaac tgtaaggacc acaatcgttg ccgcaacggc
      481 aaccattaac ggcaaggatg tggagttgct gaaagcgggg gcaattgaac aaaagtggta
      541 acgctgctca gcccccggct aaggcaaaac ttgccctggc gggtggcgat aataatctaa
      601 gccgcgagaa tatgctgaat cgccaaaaac ataggtacag caccaaaagc atttggtgtc
      661 gctgaaagct gaacaaagcc gagacgagca taaaaattaa tggcggcagg gtcagcatca
      721 agataaaccc ctttaattgg taatgcctgg tgaattattt ttacatgttc aaaaaaatca
      781 catagtaggt cctgaccaaa gccccgcttt tgatacttcc gtgctacccc caacatgact
      841 aatctgacaa caccaatttc tgaaggttgt gaaccctgaa ggacgccaga aacgcgttgt
      901 ttttccagcg aatatgccgt aaaagtacag atgccgatca gttcaccact ttgtctgtca
      961 ataagtgctt tagccgcaca gtcgctatta cgcacacttt tctttagcga tgcgcgtaca
     1021 aatttatcga taacagagtt accgcaatca aactgttttt gcccaggata agtaatatca
     1081 gactgataat tacaggagag gactatgggc agagtaatct cttcatgctt gtcgaccatc
     1141 gctgtttttc cttttcatta acgccttgag ggcgagcgtc ggttgagcag gttcagcgat
     1201 gagttggttc atcagttccc agctttgatt cgaaagctca cggcgacgtt ggttatcaag
     1261 cacactttcg gcgcgttcca tagcagcgct caaaataaaa gcgcttaaat ctaccccaac
     1321 ggcttgcgca gcttcacgaa gtttgttttt ggcgtcggga ctagttttga gttcgacccg
     1381 cgcatttttc agcgttttat cgacatcggg tagagtggct gtttttccag gcattatgac
     1441 ctctttgatt aactgaatga aatcgtaggg agtcgatctc atacataata cggctatcaa
     1501 ccgtatgatt aatttatgtt catctgctca ctctgtcaag acatacggtt attagccgta
     1561 tctctgatct cagacaataa aaaaggtgcc gtagcacctt tttaatagag aggttgtgtt
     1621 accaaacagc agccagcagc gtatgcgagt ccggcaccat aaaatcaacg gacatcatca
     1681 ccgagagggc tgtgatggcg atgatagaga agccgaacag cttgcgcgcc cagatcctgt
     1741 catcagcaac tttataaccg cgcagggcca tacctaacca ccagacgctc accgccgcgg
     1801 cgaccaccag atatttatac ccagcgtaac cgccaagaga gagcatcagc gtggcaacgg
     1861 caaaggcgat gatatacagc gtgatgtgat tcttcgccac cgaaatgcct tttaccactg
     1921 gcaataccgg aatgtttgcc gcctgataat ccttaaagcg gaaaatggcg atggcatagg
     1981 agtgaggcat ctgccacagg ctgaagatag ccagcaggat cgctgcgccg ctatcgaact
     2041 cgccggttac tgcacagtag ccaatcaccg gcggcgcagc gccggagagc gaaccaatca
     2101 atgtgccgta gaccgagtgg cgtttcatgt acaggctata aacgccgaca taaaccacaa
     2161 agcccatcac ccccagccag caggccagcg gattcgcgcc aaaccacagc agcataaagc
     2221 cagcaaagcc tagcaaggtg gcgtacacca gcgagacagc aggagagatc aggcctttaa
     2281 ccagcacccg attcttcgtc ctttccatct ttctgtcgat atccctgtcg atgtagttgt
     2341 taaacacaca acccgacgcc acaaccagtg acaccccaac cagcgtgtag ataaacaggg
     2401 gataatcaat gctgcccttt gaggccagca ggaatccccc aatcaccgag atcaggttgc
     2461 caaagatgat gcctggtttc gttacttgca ggtattgctt aaacatcata accgccgctc
     2521 ttagtgcatc atcatgttgt agttgaggtt ccacataatc cagatggagc ctacaaccag
     2581 gatagcgatg attagcacgg tgaagacaaa cgccgtcatg ttccagcctt catctgattt
     2641 ggtattcatg tgcaggaagc acaccagatg caccagaacc tgtaccactg ccattgccag
     2701 gattgttccc agaattacgg ccggagaggc agctcctgtc atcaccatcc agaacggaat
     2761 caccgtcaga atgatcgaca ggataaagcc tgtcatgtag gtttttacgc tgccatggga
     2821 cgcgccgctg tgatcggtag aatgactcat tacatcgccc ccatcagata aacaacagtg
     2881 aacacacaga tccaaaccac atccaggaag tgccagaaca ggctcaggca catgatgcgg
     2941 gtacggttag tgctggtcag gccgcgacga gcgatttgta ccatcagcac cgccatccag
     3001 ataagaccgg aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa cgctgacagg
     3061 aagccgctgc gatccggacc catgccgtta acaatcaggt gatggaattc gtagatttcc
     3121 atcccgataa atccggcacc aaacaaccag gtcaacgcca gccaggagat aacctggctt
     3181 ttgttgtttt tgtacatggc gatagccgcc atgccgtagg tgatggagct gaacaacagc
     3241 aagaaagttt caaccagaac gaacggcagt tcgaaaatgt ccttacctgt cgggccgcct
     3301 gcggtgccgt tcaccagaac ggcataggta gcaaacaaga ttgagaacag aatgcagtcg
     3361 ctcatcaggt agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg gtgcccgtgt
     3421 tcgtgcgcgt gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca gccctgcctt
     3481 agtaatctca tcgaaatgct ggttttccag tttttcgatt tctgccaccg gcacgtagta
     3541 atccacgtcc tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg cgaagccaac
     3601 aatcgccagc caccagatat gccagatcat ggcgaaaccg aagatggtgg agaaagctgc
     3661 aatgacgata cctgcaccgc tgtttttcgg catatgaatt tcttcatagt ggtcaggctt
     3721 tttatacgct tcgcctttct ctttcatttc ccagaatgca tcacgctcgt gaacgtgcgg
     3781 cacaacggca aagttataga acggaggcgg ggaagaggtt gcccactcca gcgtacggcc
     3841 accccacggg tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag aaacgtacat
     3901 ctgaataacg aggcagagaa tacccagcgc aatcagtact gcaccgctag ctgcaatcat
     3961 cagcatggtg tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca tgcccatgaa
     4021 gcccagcgca tacagcggca taaaggcaac gaagaagccg atgatccaga accagaacgc
     4081 gcgtttaccc caggtttcat tcagtttgaa accgaacgct ttaggccacc agtaggtcat
     4141 ccctgcgaag cagccgaaga ccacgccgcc gatgataacg ttatggaagt gcgcaatcag
     4201 gaacaggctg ttatgcagaa cgaagtccgc gcccggtact gccagcagca cgccagtcat
     4261 cccgcccacc gagaaagtga cgataaaacc gatggtccac agcatcgcag aatggaacac
     4321 gatgcggccc tgatacatgg tgaacagcca gttgaagatc ttcaccccgg tcgggatggc
     4381 gataatcatt gtggtgatac caaagaaggc gtttacgttc gcgcccgcac ccatcgtaaa
     4441 gaagtggtgc agccaaacga tgaacgacag cacggtgata cagacggttg cccataccag
     4501 cgaggtataa ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg agaacacacc
     4561 gaaaacaggc aggatcagga tgtaaacttc cgggtggccc caggcccaaa tcaggttgat
     4621 gtacatcatc atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca gatagcgatc
     4681 cagggtcaac aacgcgacgg taaccgtcag aattgggaag gaagcaataa tcagtacgtt
     4741 cgcgcacagt gatgcccagg taaatactgg catcttgaac atggtcatgc ccggtgcgcg
     4801 catcttcaga atggtaacga agaagttgat accggtaagc gtcgtaccta taccggatag
     4861 ctggagactc catatccagt aatcgacacc gactcccgga ctgtactcta ttcccgatag
     4921 cggtggatag gccagccagc cggtctgcgc aaattcgccc acgccgagag aaacgttaac
     4981 cagaatcaca ccaacaacag taaaccagaa gcttaagttg ttgaggaacg ggaacgcaac
     5041 gtcacgcgcg ccgatctgca gcggaaccac caggttcatc agaccgataa cgaaaggcat
     5101 cgccacgaag aagatcataa tcacgccgtg cgcggtgaag atctgatcgt agtggtgagg
     5161 tggcaggaag cccgcttcgc ccgccgaggc aagagcctgc tggctacgca tcataatggc
     5221 gtcagcaaaa ccacgcagca acatcacaat cgccacgatg atatacatga taccgaggcg
     5281 tttatggtcg acggaggtca gccactcttt ccacaggtag gtccacttac cgaagtaagt
     5341 gatcaggcca acgagcgcca gacctcccaa aataatgcca gcgatcgtaa ccatgacgat
     5401 aggttcatgg aacgggactg catcaagtga taattttccg aacatcttta ttcttcctca
     5461 acccctttaa tgggcggatt ccgcgtggct catgtccatg ccttccatac cttcgtgtgc
     5521 actgtgctca ccttctggct gggtcatgtc catgctctta ccgtgagcca taaacttgtt
     5581 aatcacatcg gcaaacaagt ctggtttcac gttggagaaa tattccacct ggttgtattc
     5641 gctaggcgcg gccagttttt cgaacgcagc catgtcagac atggtgttcg gcgactgttt
     5701 cgcttttgcg acccattggt cgaattcggc gcgatccggt gttgcaatag ctttgaactt
     5761 catgcctgag aagcccggcc cgctgtagct ggcggagata ccgtcataag taccgggttc
     5821 gttggcgatc agatgcaggc gagtctgcat accggccatg gcataaatct ggctacccag
     5881 acgcggaatg aagaaggagt tcatcacgga gttggaggtc actttgaagt acaccggagt
     5941 gttcgccggg aaagcgattt cattcacggt tgcaatgccc tgttccgggt agatgaagaa
     6001 ccatttccag tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt gtgccagcgg
     6061 cttgctaggc tcaagagcgt gagtggtttt ccaggtcagt accgcaagga agatgatgat
     6121 taagataggt accgtccaga ccacagcttc cactttattg gagtgtgacc agttcgggct
     6181 gtacttagca tctttattgc tcgcacgata cttccaggcg aaaccaacag ccatcaagat
     6241 tgcgggaata acgacaatca acatcaggcc aaatgccgtc agtatcagtg aacgttgctc
     6301 cagaccaatc tgtcctttgg gatctaacag cgcagaatta cagccactga gcaatacagt
     6361 gcctgcaaat aatgacaacc atcccaaact tttattgtat ttcctgagtc tcatttaacg
     6421 acctcaattc cacgggatct ggtggcgttt aaagtgtgtg ggcattttac ggggaggttg
     6481 cattactgta aacatgatta aattcctgtt acttggattt ggctgtcagg ttacatatgt
     6541 tgcaaaacat atcaagttga ttttaagaag ctgttgcaaa agggcgttat aacgaaaggg
     6601 gagtgcag
//
LOCUS       DAZOZY010000208         6602 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZOZY010000208 DAZOZY010000000
VERSION     DAZOZY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6602
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.209"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            152..838
                     /locus_tag="ACHURQ_003625"
     CDS             152..838
                     /locus_tag="ACHURQ_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR0825312.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(929..1065)
                     /locus_tag="ACHURQ_003626"
     ncRNA           complement(929..1065)
                     /ncRNA_class="other"
                     /locus_tag="ACHURQ_003626"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1110..1197)
                     /locus_tag="ACHURQ_003627"
     tRNA            complement(1110..1197)
                     /locus_tag="ACHURQ_003627"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1161..1163),aa:Ser,seq:gga)
     gene            1431..2369
                     /gene="ghrA"
                     /locus_tag="ACHURQ_003628"
     CDS             1431..2369
                     /gene="ghrA"
                     /locus_tag="ACHURQ_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR0825313.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2424..3161
                     /gene="ycdX"
                     /locus_tag="ACHURQ_003629"
     CDS             2424..3161
                     /gene="ycdX"
                     /locus_tag="ACHURQ_003629"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR0825314.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3185..3739
                     /gene="ycdY"
                     /locus_tag="ACHURQ_003630"
     CDS             3185..3739
                     /gene="ycdY"
                     /locus_tag="ACHURQ_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR0825315.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3841..4332
                     /locus_tag="ACHURQ_003631"
     CDS             3841..4332
                     /locus_tag="ACHURQ_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR0825316.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4397..5230)
                     /gene="csgG"
                     /locus_tag="ACHURQ_003632"
     CDS             complement(4397..5230)
                     /gene="csgG"
                     /locus_tag="ACHURQ_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR0825317.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5257..5673)
                     /gene="csgF"
                     /locus_tag="ACHURQ_003633"
     CDS             complement(5257..5673)
                     /gene="csgF"
                     /locus_tag="ACHURQ_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR0825318.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5698..6087)
                     /gene="csgE"
                     /locus_tag="ACHURQ_003634"
     CDS             complement(5698..6087)
                     /gene="csgE"
                     /locus_tag="ACHURQ_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR0825319.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6092..>6602)
                     /gene="csgD"
                     /locus_tag="ACHURQ_003635"
     CDS             complement(6092..>6602)
                     /gene="csgD"
                     /locus_tag="ACHURQ_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR0825320.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         1657 a         1512 c         1637 g         1796 t
ORIGIN      
        1 aggcaagccg caatggctaa atagggatgg ttgtaataat caaaacatga tactaactat
       61 atattgatcg ttgcattgtt ggcatgtaac acgctgcagc gagtaacatt ggtaatgaaa
      121 tataatcaac atggataggt gaaaattaat tatgagcttt atggcttttg taacttgttt
      181 gacaattgca tttatcgccg gttttgctgt tgccagaaaa ttgacactcc gaaaagttgc
      241 ctcatcggta atcatcgcta aatcgcaacc cgtcccttca tctgcaactt cccctgccgc
      301 taaggcgtcg acaaggagct atataccgca gaaaacctat cgtgactctc gttggcaaga
      361 gcgagatgag ttatttcagg gaatggttgc cgaaggtatt ttattggaaa gaaaagagtg
      421 gttaatcacg cgttgtgaaa aaatgtattt cgataatctg gaaaaatggt ttggacatta
      481 ttgtcatatc cattgccagg tctctctggg gcgcttgatc aactttccag aacaacctga
      541 gtttagcgaa gaagaaagaa agcgtttctt tgcaatttat aatggcatgg caatggatta
      601 tgttctggtt tcaaagaaga acaataaaat agtctgtgtt attgagctgg atgacccgag
      661 tcatgaaagg gcggatcgca ttattcgtga ccgttggttg gatacaatta tgactcttgc
      721 acgggtgcct tttgtacgag cttctgtcac taaattggag gaaattcctc cggtctggga
      781 gactcgtgac aaaattcgcc cattagtcgc ggcgacaaag agtgaaccgg tgcattaact
      841 aagtatcccg gtcggggctt ctcatccccc cggtgtgtgc aatatacgaa aaaaagcccg
      901 taccttcgta cgagctcttc tttaaagatg gcggtgaggg ggggattgac tcgcttcgct
      961 cgccctgcgg gcagcccgct cactgcgttc acggtctgtc caactggctg tcgccagttg
     1021 tcgaaccccg gtcggggctt ctcatccccc cggtgtgtgc aatatacgaa aaaaaagccc
     1081 gtactttcgt acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat
     1141 acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa
     1201 ttgtcgttcc tgtcttgctg gaacgggcgc taatttaggg aaatcatgac ctgaggtcaa
     1261 caaacttttt gaaaaaatcg cgcgtttatt caaacttcaa tcaatgtgtg gttttaataa
     1321 gcgaaatctg ctttttttgc caccgaccac ggatttgtta tgctggtggc ctttgtagat
     1381 cataacgata agtgcgaata aatttcgcac aacgcttttc gggagtcagt atggatatca
     1441 tcttttatca cccaacgttc gatacccaat ggtggattga ggcactgcgc aaagctattc
     1501 ctcaggcaag agtcagagca tggaaaagcg gagataatga ctctgctgat tatgctttag
     1561 tctggcatcc tcctgttgaa atgctggcag ggcgcgatct taaagcggtg ttcgcactcg
     1621 gggccggtgt tgattctatt ttgagcaagc tacaggcaca ccctgaaatg ctgaaccctt
     1681 ctgttccact ttttcgcctg gaagataccg gtatgggcga gcaaatgcag gaatatgctg
     1741 tcagtcaggt gctgcattgg tttcgacgtt ttgacgatta tcgcatccag caaaatagtt
     1801 cgcattggca accgctgcct gaatatcatc gggaagattt taccatcggc attttgggcg
     1861 caggcgtact gggcagtaaa gttgctcaga gtctgcaaac ctggcgcttt ccgctgcgtt
     1921 gctggagtcg aacccgtaaa tcgtggcctg gcgtgcaaag ctttgccgga cgggaagaac
     1981 tgtctgcatt tctgagccaa tgtcgggtat tgattaattt gttaccgaat acccctgaaa
     2041 ccgtcggcat tattaatcaa caattactcg aaaaattacc ggatggcgcg tatctcctca
     2101 acctggcgcg tggtgttcat gttgtggaag atgacctgct cgcggcgctg gatagcggca
     2161 aagttaaagg cgcaatgttg gatgttttta atcgtgaacc cttaccgcct gaaagtccgc
     2221 tctggcaaca tccacgcgtg acgataacac cacatgtcgc cgcgattacc cgtcccgctg
     2281 aagctgtgga gtacatttct cgcaccattg cccagctcga aaaaggggag agggtctgcg
     2341 ggcaagtcga ccgcgcacgc ggctactaat aaagcatcag gattcctgct atccttggcg
     2401 ggaattgaat acaggagaga gttatgtatc ccgtcgacct tcatatgcat accgttgcca
     2461 gcacacatgc atatagcaca ttgagtgatt acattgccca ggccaaacaa aagggcatta
     2521 aactttttgc gatcaccgat catggcccgg atatggaaga tgctccgcat cactggcact
     2581 tcattaacat gcgtatctgg ccgcgagtgg ttgatggggt agggatcctg cgcggcatcg
     2641 aagctaacat taaaaatgtt gatggtgaaa ttgattgcag cggtaaaatg tttgactcgc
     2701 tggatctgat tattgccggt tttcatgagc cggtttttgc accacatgac aaagcgacca
     2761 atacacaagc gatgatcgcc actatcgcca gcggcaatgt gcatataata agccatcccg
     2821 gaaatcccaa atatgaaata gatgtgaaag ccgttgctga agcagccgcg aaacatcagg
     2881 tggcgctgga aatcaataat tcttcatttt tacactcacg taagggcagt gaagacaact
     2941 gtcgtgcggt agccgcagcg gtacgtgatg ctggaggttg ggtggcatta ggctcggatt
     3001 ctcacaccgc gtttaccatg ggggaatttg aagagtgtct taaaatcctc gacgcggtag
     3061 attttccgcc agagcgcatt ttgaatgttt ctccgcgccg cttactgaac ttccttgaat
     3121 ctcgcggtat ggcaccgatt gcggaatttg cagaccttta attactagta atggaaatat
     3181 attaatgaac gagttttcta tcctctgtcg tgtgctgggt tcgctctatt accgccaacc
     3241 gcaagatcct ttactggtgc cgctgtttac cctgattcgt gaggggaaac tggctgcgaa
     3301 ctggccactg gagcaggatg agttactgac gcgtttacag aaaagctgtg atatggcgca
     3361 agtctctgcc gattacaatg cgttgtttat cggcgatgaa tgtgcagtgc cgccatatcg
     3421 tagcgcatgg gttgaggacg taacggaagc ggaagtgcgc gcttttcttt ccgaacgagg
     3481 gatgccactg gcggatacgc cagccgatca catcggcaca ttgctgcttg cggcttcctg
     3541 gctggaagat cagtcaacgg aagatgagag cgaagcactg gaaacactgt tcagtgagta
     3601 tctgttaccc tggtgtggtg cgttcctcgg caaagtggag gcccatgcaa ccacgccttt
     3661 ctggcgcacc atggcaccgc taacccgtga tgccattagt gcaatgtggg acgagctgga
     3721 agaagattct gaagagtaaa tgtgatcatc gtcactcata actgcaacag ataatttgtt
     3781 gttgcataaa atgtgtgctc gatctcattc atggccgcgt tttctgctat catgcgcggc
     3841 atgaacatac ttctctctat tgcaatcaca acaggcattc tctccggtat ctggggatgg
     3901 gtggctgttt ccctgggctt actaagctgg gcgggcttct tgggctgtac ggcctacttt
     3961 gcctgcccgc aaggtgggct gaaagggctg gcgatctccg ctgcaaccct gcttagtggc
     4021 gtggtgtggg cgatggtcat tatttacggt agtgcactgg caccacatct ggaaattctc
     4081 ggttatgtca taaccggtat tgtggcgttt ctgatgtgta ttcaggccaa acagctgctg
     4141 ctttcatttg ttccggggac gtttataggc gcatgcgcga cgtttgccgg gcagggtgac
     4201 tggaaactgg tgttaccttc gctggcgctt gggctggtat ttggttacgc aatgaaaaac
     4261 agcggcctgt ggctggcggc gcgtagtgca aagacggctc accgtgagca ggaaatcaaa
     4321 aataaagcgt gaggggcact cacgctttcg cttaaacagt aaaatgccgg attattaatt
     4381 ccggcatttt tatctgtcag gattctggtg gaaccgacat atggcggtat ttaaccagaa
     4441 tgtcattctg ccgttctgct ttgttctgca aatcccacag accacggtcg ataccatcat
     4501 taatcaggaa aatgacccct gtttcgatag ccgacatcag acacagcata acgggttcgt
     4561 ttgaggtgta acccacttcc ccttccagca agcgctggta gtcaataaag cggaaaaccc
     4621 cggcctgaac ttcataggaa agtatcgtct tactggtgtt caccgaggag aggatctcac
     4681 cggtactcac attgacgaca cgcaggttca cggcaatctg atcgagctgg tattgcgtgt
     4741 ctgcaccgat cccaaaatat cttgccccaa caccgcccga tttaacgttg ctttcataac
     4801 cgataatcga accttcaacc atgatatttg ccgccgttaa agattgcagc gggattcggt
     4861 tattaatggc aactgtgccg ttttcttgtg ccgcacgaat aatcttgcgc tcgttaagca
     4921 ggttttgtaa actctgacgc tccagcggta taaaccagcg agaatctttc aatgccgtga
     4981 ccagcattgc cgtggcgctt tgcggaacag cagtggagaa gttacttgcc gggtagggtt
     5041 taaattgccc ggtttcgtcc tgaatgttgt ataccgaaac aaagatctta cccgtcggcg
     5101 ctggcagatg ggtcaaatct ttgtagctct gagctcgagg cattaatgtc ggtctggcgg
     5161 cttctttagg cggggcggtt aagcatccgc tcagtaacat gacggcaacc aaaagaaata
     5221 agcgctgcat gattattttc cttatgaagc tggggcttaa aaatcggttg agttattttg
     5281 taaacccgaa acctggatgg tcgaggtttg tccggtttta cgatctgtca cgttcaactg
     5341 caattgacca tcgcggttag caatatcaac aatataatcg ttggtcacca tgcggcccgg
     5401 tttaccggta ttaatattcg acagtagccc acctaaaatt tgtgactgga tggcctgagt
     5461 aaagttatct aacgctgagg gtgtttcaat accaaagtca tcgttatagc tcggatcttt
     5521 ataagagttt tgggcctgag cgctatttaa taaaaaagcg ccattatttg ggttaccacc
     5581 aaagtttgga ttacggaact ggaaagtcat ggttccagcc caacttaatg gcgaaataag
     5641 catgagtaga actactgcat gtttgacacg cattgcagcc tccgaacaat tttttattta
     5701 gaattcatca tgcgccaaat cgcccgtact taataacgcc tgatttatct ggcgacgatt
     5761 tagtgcttct tcagtttgaa tcagtgcaaa gacgacagtt ttctcgaagt ctcttttcaa
     5821 cggaaataaa aaagtctgga aaataacgtc ctgattgacc gttatagtga tccagcttcc
     5881 ccatcgtgca ctgggccttt cattaatcgt taagttaccc gtatagtcac tttcccattt
     5941 atcactaaag gctcggtaaa aatcatggcc aatagatgaa acagtatggt cagttagcaa
     6001 tcccgggact tctacctcaa cggcgtgaag attccctgcg gcgaacagaa attctgccgc
     6061 cacaatccag cgtaaataac gtttcatggc tttatcgcct gaggttatcg tttgcccagg
     6121 aaaccgcttg tgtccggttt tttacggcta tcttcttgaa aagattataa agatgcgttt
     6181 taaccgtatt ttcgctgatg aacaacgaac gagcgatctc gttattagac gcgccgatac
     6241 gcagtttatt caggatctct ttttcccgat gagtaaggag ggctgattcc gtgctgttat
     6301 aacgatagtt acctgaatgc gtaatcaagt agctggcaag cttttgcgta aagtagcatt
     6361 cgccgcgcag gacgccttgc aacccattga caacacgttc ttgatcctcc atggcataaa
     6421 aaacgccgtt gatatgaggc cagttttcaa tgtcgcggta cgggtaatct tcaggcgtat
     6481 ttagcaacaa tattttgata ttgttgtttt tcctgctcaa agtatcctgc caataatgga
     6541 taagcttttt atccgcttcc atcatatcca gaagaataat agagcctgaa gatatatcgt
     6601 cc
//
LOCUS       DAZOZY010000209         6554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZOZY010000209 DAZOZY010000000
VERSION     DAZOZY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.210"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            143..1318
                     /gene="mdtL"
                     /locus_tag="ACHURQ_003636"
     CDS             143..1318
                     /gene="mdtL"
                     /locus_tag="ACHURQ_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR0825321.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1293..2252
                     /gene="yidZ"
                     /locus_tag="ACHURQ_003637"
     CDS             1293..2252
                     /gene="yidZ"
                     /locus_tag="ACHURQ_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR0825322.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2409..3158
                     /locus_tag="ACHURQ_003638"
     CDS             2409..3158
                     /locus_tag="ACHURQ_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR0825323.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MGLQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3180..3746
                     /gene="yieF"
                     /locus_tag="ACHURQ_003639"
                     /gene_synonym="chrR"
     CDS             3180..3746
                     /gene="yieF"
                     /locus_tag="ACHURQ_003639"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR0825324.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3800..5137)
                     /gene="adeP"
                     /locus_tag="ACHURQ_003640"
     CDS             complement(3800..5137)
                     /gene="adeP"
                     /locus_tag="ACHURQ_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR0825325.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5303..5968
                     /gene="yieH"
                     /locus_tag="ACHURQ_003641"
     CDS             5303..5968
                     /gene="yieH"
                     /locus_tag="ACHURQ_003641"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR0825326.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6035..6508
                     /locus_tag="ACHURQ_003642"
     CDS             6035..6508
                     /locus_tag="ACHURQ_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR0825327.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1609 a         1655 c         1659 g         1631 t
ORIGIN      
        1 aatttggcta aatccttcaa gaagccagcc attcgctggc ttcttgcctc tcaggaaatc
       61 acttatgtcc aaatggcaac tcgcctgatc ctccttcacc acgtatgctt tgcgtcacct
      121 tactatcagg acgctttagc ccatgtcccg ctttttgatt tgtagttttg ccctggtttt
      181 actttatccc gccgggattg atatgtacct cgttggttta ccgcgcatcg ccgccgatct
      241 caatgccagc gaagcgcagt tgcatattgc gttctccgta tatctggcgg ggatggcagc
      301 tgcgatgtta tttgccggta aagtggctga tcgttcaggg agaaagccgg tcgccatacc
      361 cggcgcggcg ctatttatta ttgcctcggt gttctgttca ctggctgaaa ccagcacgtt
      421 atttcttgca ggccgatttc tacaggggtt gggcgcaggc tgttgttacg tagtggcgtt
      481 cgccattttg cgcgacacgc tggatgatcg acgtcgggct aaagtgctgt cattactcaa
      541 cggtattacc tgcatcattc cggtgttagc gccagtgctc ggacatctga ttatgcttaa
      601 attcccgtgg cagagtctgt tctgggcgat ggcaatgatg ggcatcgcgg tactgatgtt
      661 gtctttgttt attttaaaag aaacgcgccc agcgtccccc gctgcttcgg acaaaccacg
      721 agaaaatagc gagtcgctgc ttaatcggtt tttcctcagc cgtgttgtta tcaccaccct
      781 cagcgtttcg gtgatcctca ctttcgtcaa tacatcgccg gtattgctga tggaaatcat
      841 gggttttgag cgcggagaat acgccaccat tatggcgttg accgctggcg tcagcatgac
      901 cgtttcattc tccacgccat ttgcgctggg aatttttaag ccacgtacgt tgatgatcac
      961 ctcgcaggtg ttattccttg cggcggggat cacccttgcc gtttcacctt cccatgcggt
     1021 ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat
     1081 gagtcaggcg ttaggaccat tttcattacg cgcgggcgta gccagctcga ccttaggtat
     1141 tgcgcaggtt tgcggttcgt cactgtggat ttggctggca gcggtggttg gtattggcgc
     1201 atggaatatg ctgatcggga ttctgattgc ctgtagtata gtgagcctgt tgctgattat
     1261 gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa atccatcacc acgcttgatc
     1321 tcaatctgct gctctgtctg caactgctga tgcaggagcg cagcgtgacc aaagcggcga
     1381 agcggatgaa cgtgacccct tcagcggtga gtaagtcgct ggcaaagtta agagcgtggt
     1441 ttgacgaccc gctctttgtg aactcaccgc tgggcctgtc gcccacaccg ctaatggtca
     1501 gcatggagca aaatctggcg gaatggatgc aaatgagcaa tctgctgctg gataaaccgc
     1561 accaccagac accgcgcggc ctgaagtttg agttggcggc ggaatcgccg ctgatgatga
     1621 tcatgcttaa tgcgctgtcg aaacggatct accaacgtta cccgcaggcg accatcaaac
     1681 tacgtaactg ggattacgat tccttagatg ccattactcg tggtgaagtg gatattggtt
     1741 tttccggtcg cgaaagccat cctcgctcgc gggagctgtt aagctcgcta ccgttagcca
     1801 ttgattatga agtgctgttt agtgatgtac cctgcgtctg gttacgcaaa gatcatccgg
     1861 cactgcatga aacgtggaat ctggacacct ttttacgtta tccgcatatc agcatttgct
     1921 gggaacagag cgacacctgg gcgctggaca atgtgttaca ggagctgggg cgcgaacgca
     1981 cgattgctat gagtctgccg gaattcgagc agtcactgtt tatggcagcg caacccgaca
     2041 atctgctact ggcgaccgcg ccgcgctact gtcagtacta caatcaactc catcaactgc
     2101 cgttggttgc tcttcctctc ccgtttgacg aaagccagca aaaaaagctg gaagttcctt
     2161 ttaccctgct gtggcataaa cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa
     2221 ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg tatgaaacgc gacccaattt
     2281 cacaataaaa tgtaaaaaag ttgtaacaag cttgtctgaa tcgaactttt agccgcttta
     2341 gtctgtccat cattccagta aatgattact cttgtattca taatggacca ttaagcatgg
     2401 agcgaaaaat ggcgactcac tttgcccgag ggattttaac ggaaggacat ctgatttctg
     2461 ttcgtctccc ctcccagtgt catcaagaag cccgaaacat tccccctcat cgtcaaagcc
     2521 gttttctggc gtccagaggt ttactcgcag aactgatgtt catgctgtat ggcattggcg
     2581 aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc
     2641 cttcgttttc tatttcctat gccgggaata tggttggcgt ggcgttaaca accgaaggtg
     2701 aatgtggcct cgatatggga ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc
     2761 ccgataacca caccttttcc agcaatgaat cgctatggat cagtaaacaa aacgatccta
     2821 acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt gctaaaacta accggtgatg
     2881 ttttgaatga cgatccgcgc gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg
     2941 ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg
     3001 cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt agatggcaaa cacagctgga
     3061 aaagcctgcc ggatattcac agccgcgcca acaatcctac cagccggatg atgcgttttg
     3121 cccagctctc taccgtgaag tctttttcgc caaattgata gacaaccaca ggagtcatta
     3181 tgtctgaaaa attgcaggtg gttacgttac tggggagcct gcgcaaaggc tcatttaatg
     3241 gcatggttgc acgtaccctg ccgaaaattg ctccggcgag catggaagtc aatgcgttac
     3301 cttccattgc cgacattccc ctgtatgacg ctgacgtaca gcaggaagaa ggttttccag
     3361 caacggttga agctctggcg gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc
     3421 cggaatataa ctactcggta ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc
     3481 tgccggatca accgctggcc ggtaaaccgg tgttgattca gaccagctca atgggcgtga
     3541 ttggcggcgc gcgctgtcag tatcacctgc gccagattct ggttttcctc gatgcaatgg
     3601 tgatgaacaa gccggaattt atgggcggcg tgattcagaa caaagttgat cggcaaaccg
     3661 gagaagtgat tgatcagggt acgctggacc acctgaccgg gcaattgacc gcatttggtg
     3721 agtttattca gcgagttaag atctaaataa aaaacccgcc agtaatcatg catggcgggt
     3781 ttttaatgcg ctatcgattt taatgagcgt cgataaatac aatcttcagg ataaacagca
     3841 gcgcaacgat gattacgcac gggctaaggt cacgcagacg cccggtacca atcttcatca
     3901 cgcagtagga gataaagccc agcgcaatac cttcggtaat cgaaaagctg aacggcatca
     3961 tcacggcggt aataaacgcc ggaacagatt cagtaagatc ctgccagttc acgcgagcaa
     4021 gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc agctgcagcg tagcctggca
     4081 ccattcccgc cagcggcgac agaaagataa ccagcaggaa cagcagacca acaaccactg
     4141 ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga ggactcaata taagccgtaa
     4201 cggaagaagt accgataaac gaaccggtca cggaagagat actgtcgaca tacagcgcct
     4261 gcttcatgcg cgggaatttc cccttctcat ccgccagacc tgctttatcg gtcacgccaa
     4321 tcagcgtacc ggaggagtca aacaagttga ccaacatgaa agagaaaatc acccctgcca
     4381 gcccgaggtt aaacgacccg gctaaatcta catgacccac aactgtcatt acgctcggcg
     4441 gcgcagaaac gatgccattg tagtgcacat cacccagcat ccagcccagc agcgtcgtca
     4501 ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga ggccagaatg gcgatgatga
     4561 agaaaccgag gatccccagc agcacgctgt gagaagtcag attaccgatg ctcaccagcg
     4621 tttccgggtt agcgacaatc acacctgcgt ttttcagccc catcatgcca atgaacagac
     4681 cgataccgct ggtaataccc acacgcagac ttaccggaat gttagcaatc atccagtaac
     4741 gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc ccagaagatt gcgcccatac
     4801 cgatctgcca cggcagcccc attgcctgga caaccacaaa agcgaaaaag gcgttaagcc
     4861 ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa tcccatcata atgctaccga
     4921 aagcagcaat cagacaggtt gttacgaaga cggcactggt gtccatgcca gcaacgccca
     4981 gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag gaaggtggtg aacccggcaa
     5041 tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt aaaaactcgt tccagcatcc
     5101 cctgaccaga agtctgggtg gtatgttgat gactcattat ctatttccga acaaggaggg
     5161 aaaatccgtc gctatcgtat accaaaatgc gacaataggc gcgtttgtga gagacttttt
     5221 ttattggatt tacttatacg gcaacgattg cgttgcgcaa atcggctcta cgaaaacgtt
     5281 aaactgatta aaaaggaaag gcatgtcccg gatagaagcg gtatttttcg actgcgacgg
     5341 tacgctggtc gacagtgaag tcatttgctc ccgcgcatat gtaacgatgt ttcaggaatt
     5401 tggtattacg ctcgatcctg aagaggtatt caaacgtttc aaaggtgtaa aactgtacga
     5461 aattatcgat attgtttccc ttgaacatgg tgttacgtta gcgaaaacag aatctgaaca
     5521 cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa ctggaagcca tcgaaggggc
     5581 tgaagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg gtatctaacg gcccaaataa
     5641 caaaatgcag cattctatgg gcaagctgaa tatgttgcac tacttcccgg ataaactgtt
     5701 cagcggctac gatattcagc gctggaagcc agacccggcg ttaatgttcc atgcggcaaa
     5761 agcgatgaat gtaaatgtag aaaactgcat tctggttgat gactcagttg ccggtgcgca
     5821 atctggtatc gacgcaggta tggaagtgtt ctacttctgc gccgacccgc acaataagcc
     5881 gatcgttcac ccgaaagtca ccacctttac ccatctttcg cagttaccgg aactgtggaa
     5941 agcgcgtggt tgggatatta cggcatagtt cttcacactc ccttcactta ccccgcttaa
     6001 attggcgctc aaaggtaagt aaagggagtt tgatatgtct gtttcacgtc gggtaataca
     6061 tcacggactt tattttgcgg ttttaggacc gttaattggt gttctgtttc ttgtcctcta
     6121 catattcttc gcaaaagaac cgctggttct tttggtaata atacaggtac ttccattgtt
     6181 tctcttattg tcgattacta cgggagctat tcctgcattg ttaaccggcg tgatggtcgc
     6241 ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt tgtctggctg gtggcatagg
     6301 tggcgtcgtt atcaccgaga tctattgtgc agttattgta catattaagg gcatggcttc
     6361 ctcggagttg tttgaaaaca ttctttctgg tgacagtctc gttgtccgca tcattcctgc
     6421 attgctggca ggtgtggtga tgagcagaat cattacccgt ctgcccggat tggatatttc
     6481 atgtcctgaa acagactctt taagttaagc gggatacttt atctttgggc tactcaaaag
     6541 cagacaggat gtta
//
LOCUS       DAZOZY010000210         6538 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZOZY010000210 DAZOZY010000000
VERSION     DAZOZY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.211"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..967)
                     /gene="rfaC"
                     /locus_tag="ACHURQ_003643"
     CDS             complement(<1..967)
                     /gene="rfaC"
                     /locus_tag="ACHURQ_003643"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR0825328.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAM"
     gene            complement(971..2017)
                     /gene="rfaF"
                     /locus_tag="ACHURQ_003644"
     CDS             complement(971..2017)
                     /gene="rfaF"
                     /locus_tag="ACHURQ_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR0825329.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(2027..2959)
                     /gene="rfaD"
                     /locus_tag="ACHURQ_003645"
     CDS             complement(2027..2959)
                     /gene="rfaD"
                     /locus_tag="ACHURQ_003645"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR0825330.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            3173..4369
                     /gene="kbl"
                     /locus_tag="ACHURQ_003646"
     CDS             3173..4369
                     /gene="kbl"
                     /locus_tag="ACHURQ_003646"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR0825331.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            4379..5404
                     /gene="tdh"
                     /locus_tag="ACHURQ_003647"
     CDS             4379..5404
                     /gene="tdh"
                     /locus_tag="ACHURQ_003647"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR0825332.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            5643..>6538
                     /gene="waaH"
                     /locus_tag="ACHURQ_003648"
     CDS             5643..>6538
                     /gene="waaH"
                     /locus_tag="ACHURQ_003648"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFR0825333.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         1552 a         1636 c         1694 g         1656 t
ORIGIN      
        1 tcatagcagc gttttcttca atgaatcgct taacagcatt tgctgtcaat tgagacaact
       61 cattccccgg agccctacaa accatctgat tcttcccata tccaccaatt aatcccggat
      121 cggttggtcc ataaaccgtg atattgggtc tatccagtgc cgccgttaaa tggcttaacc
      181 ccgtatccac cgacactaca aatttagccc cggccagcac gcgggcaacg ccttccagac
      241 tcatcttcgg caatacttca acataagcaa atccttccgc cagtcgtttc gcccgctctt
      301 cctcatgcgg cgcgccccac ggaagtttaa tccgtattcc tgaatcagcc agtaaaccaa
      361 tcaattctcg ccagtgttct tccggccagt gtttatcatc acgggtcgtc gcatgaagaa
      421 atacggcata ttcgccagca tctgtaggca ggttcgtcag gaaatgctgt gcaatagcat
      481 aatcgccctg ggtttgcggt ttgctatagc ccagactttt ggcaaacagt tcgcgggtgc
      541 gttctacggc gtgctgctgt tttgcaatat gatgcttacg attgtaaaac agactggcta
      601 aaggttcgcg agcggtttgc cagtccatgc catgctttac gccatgcgcc agacgcgtca
      661 ccagcgccgc gctttttacc agcccctgag cgtcgataac tgcgtcatag ttctctgctt
      721 gtagcgcttc acgaaacgct ttgcgttccg cttttatggg ggccgagaac caggctttac
      781 gccagcgacg tattgccaca ggaataactc gctcaacggc ggcgtgccag gaaggaatct
      841 gtgcgaaccc ttcttccacc acccagtcaa acttaatccc tgggattgcc tgctgggcat
      901 cagtgagtgc gggcaacgta tggagaacat cgcccatcga cgatgtttta acgatcaaaa
      961 cccgcatccg tcaggcttcc tcttgtaaca atagcgcgtt gagttcttcc agtacgcgct
     1021 ggggagtaat gtcgattaag ctctggtgat aaccctccgc agcgtcacct ttacgcactt
     1081 tgtgatagcc ggtaatcagg cggatcacgc gcgctttatg ggatagcggc ggtgtgaagt
     1141 ccgggctact cggaccatac agggcaacca gcggacgatt gagcgccgcc gcaacgtgca
     1201 tcaggccaga atcgttagtg acaatggctt tacaggctgc aatcaggata accgcttgat
     1261 caagctgtgt ttcccccgcc aggttccgac accatgcctg ctgctcggta ttcaaagcgg
     1321 caagaatctc attgcccgct tcatgatctt tcgccgagcc aaacagaacc acctgataac
     1381 cttcatcaat cagctgcttt gccagttccg catagtggta gtgtggccag cgttttgccg
     1441 gaccaaactc cgcacccggg caaaaaccaa tcatcggacg ttctgatgaa agcgaaaatt
     1501 gattacaggt atatgatttt tcaccttcgc tcacctgcaa ttgcggccat aacaacggtt
     1561 ttggtagatc ttgtgcggta cgcatcacgc ctttgtcata ggccagcgcg acatagcgtt
     1621 ccaccattag cggccaggct tctttatcga gcacgcgtaa atcgttgagt aaaccgtagc
     1681 gcatctcgcc gcgccagccg gtgcgatgag gaatacccgc gaagaaaggc actaatgcag
     1741 atttgaagga gttgggtaag acgtaggcgc ggtcgtagcg cttttcacgc aggctatgac
     1801 ccagtttgcg gcgttcgccg atttccagcg ctccgtgacc gagaggcata ggaatagctt
     1861 cgttaacttc cggcatccgc gataataatg gacggcacca tgccggtgcc atcacatcga
     1921 ttatcgcctg gggatagcgc gcctggagcg tgcgatagag actttgcgac atcatcatgt
     1981 cgccaaccca agacgggccg atcaccagta ttttcatgca gagctcttat gcgtcgcgat
     2041 tcagccaggc catgtattcc gttacacctt cagcaacggt tttgaacggt ttgtcgtaac
     2101 ccgccgcgcg cagatttgtc agatctgcct gagtgaacgc ctggtagcgg cctttcagct
     2161 tatccgggaa cggaatgtat tcgatctggc ctttcttgtg ataagccagc gtagcatcag
     2221 ctacagcctg gaaggattcc gcacgaccag taccgaggtt gaagatgccg gaaacgccat
     2281 tttccaggaa ccacagattc acatcagcca cgtcgcccac atagacgaag tcgcgtttga
     2341 agttctcgct accttcaaac agcttcggtg attcaccgtt gttaagctgg gtgttgagat
     2401 ggaaagcgac gctcgccatg ctgcctttat ggccttcacg cggtccataa acgttgaaat
     2461 agcggaagcc aacaatctgc gagttagctt caggcaggat ttggcgaaca tattcatcaa
     2521 acaggaattt tgagtaaccg tagacgttca acggtttttc gtactcgcgg gattcaataa
     2581 agtcggaggt gcgtccgccg taggtggctg cggaagaggc atacaggaac gggatttcgc
     2641 gctccagaca gtagtgcagc agctctttgg agtattgata gttgttatcc atcatatact
     2701 tgccatccca ctcggtggtg gaagagcacg cgccttcgtg gaaaatcgct tcgacatcgc
     2761 cgaactcttc gccagccata atctggatca ggaagtcttc cttatccata tagtccgcga
     2821 tatccagatc caccaggttc acaaacttgg tgccgtcttt caggttgtcc accaccagaa
     2881 tatcggtgat gcctttatca ttcagggcct taacgatgtt gctgccgata aagcccgcgc
     2941 cgccggtaac gatgatcata actgtaacct tcgaattatg gagtcagaga caatctcaga
     3001 cacgaatact tctatcatat cactacacgg cgttggcttc agccattcgc caaaagtgag
     3061 attgatggac gttatttatc ctgaattttg cagaagtgtt aacgcgtcat ctcgtcgcga
     3121 cctataagtt tgggtaatat gtgctggaat ttgccctgtc tggagaatcg caatgcgtgg
     3181 agaattttat cagcagttaa ccaacgatct ggaaaccgca cgggcggaag ggttgtttaa
     3241 agaagagcgc attattacgt ctgcgcagca agcagatatc actgtggctg atggaagcca
     3301 cgtcattaac ttttgtgcca ataactatct cgggctggcg aattatccgg atctgattgc
     3361 ggcggcaaag gcgggaatgg attctcacgg tttcggcatg gcttcggtgc gttttatttg
     3421 cggcactcag gacagccata aagagcttga acaaaaactg gcggcattcc tggggatgga
     3481 agatgcgatt ctctactctt cctgctttga tgctaacggt ggcctgtttg aaacgctgct
     3541 gggcgcggaa gacgccatta tctccgacgc actgaaccac gcgtctatta ttgatggtgt
     3601 gcgtctgtgt aaagctaaac gctatcgcta tgccaacaac gatatgcagg agctggaagc
     3661 acgtctgaaa gaagcgcgtg aagccggtgc gcgtcatgtg ctgattgcca ccgatggtgt
     3721 gttctcgatg gacggcgtga ttgccaacct gaagggcgtt tgcgatctcg cagataaata
     3781 tgatgccctg gtgatggtag acgactccca cgcggtcggt tttgtcggtg aaaatggtcg
     3841 tggttcccat gaatactgcg atgtgatggg ccgggtcgat attatcaccg gcacgctcgg
     3901 taaagcgctg ggcggggctt ctggtggtta taccgcggcg cgcaaagaag tggttgagtg
     3961 gctgcgccag cgttctcgtc cgtacctgtt ttccaactcg ctggcaccgg ccattgttgc
     4021 cgcatccatc aaagtactgg agatggtcga agcgggtagc gagctgcgtg accgtctgtg
     4081 ggcgaacgcg cgtcagttcc gtgagcaaat gtcggcggcg ggctttaccc tggcgggagc
     4141 cgatcacgcc attattccgg tcatgcttgg tgatgcggta gtggcgcaga aatttgcccg
     4201 tgagctgcaa aaagagggca tttacgtcac cggtttcttc tatccggtcg ttccgaaagg
     4261 tcaggcgcgt attcgtaccc agatgtctgc ggcgcatacc cctgagcaaa ttacgcgtgc
     4321 agtagaagca ttcacgcgta ttggtaaaca actgggcgtt attgcctgag gatgtgagat
     4381 gaaagcgtta tccaaactga aagcggaaga gggcatctgg atgaccgacg ttcctgtacc
     4441 ggaactcggg cataacgatc tgctgattaa aatccgtaaa acagccatct gcgggactga
     4501 cgttcacatc tataactggg atgagtggtc gcaaaaaacc atcccggtgc cgatggtcgt
     4561 gggccatgaa tatgtcggtg aagtggtagg tattggtcag gaagtgaagg gcttcaagat
     4621 tggcgatcgc gtttctggcg aaggccatat cacctgcggt cactgtcgca actgtcgtgg
     4681 cggtcgtacc catctgtgcc gcaacacgat cggcgtcggc gttaaccgcc cgggctgctt
     4741 cgccgaatat ctggtgattc cggcgttcaa cgccttcaaa attcccgaca atatctccga
     4801 cgacctggct tccatttttg atcccttcgg taacgccgtg cataccgcgc tgtcgttcga
     4861 tctggttggc gaggatgtgc tggtctctgg tgcaggcccg attggtatta tggcagcggc
     4921 ggtggcgaaa cacgttggtg cacgcaatgt ggtgatcact gatgttaacg aatatcgtct
     4981 cgaactggcg cgcaaaatgg gtatcacccg tgcggtcaac gtcgctaaag aaaaccttaa
     5041 tgatgtgatg gcagaactgg gcatgaccga aggctttgat gtcggtctgg aaatgtccgg
     5101 tgcgccgcca gcgtttcgta ccatgctcga caccatgaac cacggcggtc gcattgcgat
     5161 gctgggtatt ccaccgtccg atatgtctat cgactggacc aaagtaatct ttaaaggctt
     5221 gttcattaaa ggtatttacg gtcgtgagat gtttgaaacc tggtacaaga tggcggcgct
     5281 gattcagtca ggtctggatc tctcgccgat cattacccat cgtttctcta tcgatgattt
     5341 ccagaagggc tttgacgcta tgcgttcggg ccagtccggg aaagtaattc tgagctggga
     5401 ttaacacgaa caagggctgg tattccagcc cttttgcctg aggataatcc gttaaatatg
     5461 taaaatcctg tcagtgtaat aaagatatcg taattgcgct gatctcttat atagctgctc
     5521 tcattatctc tctaccctga agtgactctc tcacctgtaa aaataatatc tcacaggctt
     5581 aatagtttct taatacaaag cctgtaaaac gtcaggataa cttcagaggt cgtcggtaat
     5641 ttatgatgaa cagcaccaat aaacttagtg ttattattcc gttatataat gcgggcgatg
     5701 atttccgcac ttgtatggaa tctttaatta cgcaaacctg gactgctctg gaaatcatta
     5761 ttattaacga tggttcaacg gataattctg ttgaaatagc aaagcattac gcagaaaact
     5821 atccgcacgt tcgtttgttg catcaggcga atgctggcgc atcggtggcg cgtaatcgtg
     5881 ggattgaagt ggcaacgggc aaatatgtcg cttttgtcga tgctgacgat gaagtctatc
     5941 ccaccatgta cgaaacgctg atgaccatgg cgttagagga cgacctcgac gtggcgcagt
     6001 gcaacgctga ctggtgtttt cgtgaaacgg gagaaacctg gaaatccatc cccagcgatc
     6061 gccttcgctc aaccggcgta ttaaccggcc cggactggct gcggatgggg ctttcttcgc
     6121 gccgttggac tcacgttgtc tggatggggg tttatcgccg cgatgttatt gttaaaaata
     6181 acattaaatt tattgccgga ttacatcatc aggatattgt ctggacaaca gaatttatgt
     6241 ttaatgcgct gcgtgcgcga tataccgagc aatcgttata taaatattat ctgcataata
     6301 cgtcagtgag tcggttgcat agacaaggga acaaaaacct taattatcaa cgtcactata
     6361 ttaagattac tcgtctgcta gagaaattaa atcgaaatta tgccgacaaa attacgattt
     6421 atccggaatt tcatcagcaa ataacttacg aagcattgcg tgtttgccat gcggtgcgca
     6481 aagagccgga tattattacc cgccaacgga tgattgccga gatatttact tccggtat
//
LOCUS       DAZOZY010000211         6499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZOZY010000211 DAZOZY010000000
VERSION     DAZOZY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.212"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHURQ_003649"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHURQ_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHURQ_003650"
     CDS             complement(1751..2509)
                     /locus_tag="ACHURQ_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR0825334.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHURQ_003651"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHURQ_003651"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR0825335.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHURQ_003652"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHURQ_003652"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR0825336.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHURQ_003653"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHURQ_003653"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR0825337.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHURQ_003654"
     CDS             complement(5474..6079)
                     /locus_tag="ACHURQ_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825338.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6499)
                     /locus_tag="ACHURQ_003655"
     CDS             complement(6082..>6499)
                     /locus_tag="ACHURQ_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825339.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1638 a         1695 c         1663 g         1503 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgt
//
LOCUS       DAZOZY010000212         6412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZOZY010000212 DAZOZY010000000
VERSION     DAZOZY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.214"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            67..1122
                     /gene="phoE"
                     /locus_tag="ACHURQ_003656"
     CDS             67..1122
                     /gene="phoE"
                     /locus_tag="ACHURQ_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0825340.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1161..1562)
                     /gene="crl"
                     /locus_tag="ACHURQ_003657"
     CDS             complement(1161..1562)
                     /gene="crl"
                     /locus_tag="ACHURQ_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0825341.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="ACHURQ_003658"
     CDS             complement(1620..2864)
                     /gene="frsA"
                     /locus_tag="ACHURQ_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0825342.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="ACHURQ_003659"
     CDS             complement(2956..3414)
                     /gene="gpt"
                     /locus_tag="ACHURQ_003659"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0825343.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3675..5132
                     /gene="pepD"
                     /locus_tag="ACHURQ_003660"
     CDS             3675..5132
                     /gene="pepD"
                     /locus_tag="ACHURQ_003660"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0825344.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5189..5803)
                     /gene="prfH"
                     /locus_tag="ACHURQ_003661"
     CDS             complement(5189..5803)
                     /gene="prfH"
                     /locus_tag="ACHURQ_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0825345.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5800..6412)
                     /locus_tag="ACHURQ_003662"
                     /pseudo
     CDS             complement(5800..6412)
                     /locus_tag="ACHURQ_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
BASE COUNT         1631 a         1645 c         1578 g         1558 t
ORIGIN      
        1 actttcctgt tttcaacggg gctctcccgc tgaatattcg cgcgttaatt aaaatcagga
       61 atgaaaatga aaaagagcac tctggcatta gtggtgatgg gcattgtggc atctgcatct
      121 gtacaggctg cagaaatata taataaagac ggtaataaac tggatgtcta tggcaaagtt
      181 aaagccatgc attatatgag tgataacgcc agtaaagatg gcgaccagag ttatatccgt
      241 tttggtttta aaggtgaaac acaaattaac gatcaactga ctggttatgg tcggtgggaa
      301 gcggagtttg ccggaaataa agcggagagt gatactgcac agcagaaaac gcgtctcgct
      361 tttgccgggt tgaaatataa agatttgggt tctttcgatt atggtcgtaa cctgggcgcg
      421 ttgtatgacg tggaagcctg gaccgatatg ttcccggaat ttggtggcga ttcctcggcg
      481 cagaccgaca actttatgac caaacgcgcc agcggtctgg cgacgtatcg gaacaccgac
      541 ttcttcggcg ttatcgatgg cctgaactta accctgcaat atcaagggaa aaacgaaaac
      601 cgcgacgtta aaaagcaaaa cggcgatggc ttcggcacgt cattgacata tgactttggc
      661 ggcagcgatt tcgccattag tggggcctat accaactcag atcgcaccaa cgagcagaac
      721 ctgcaaagcc gtggcacagg caagcgtgca gaagcatggg caacaggtct gaaatacgat
      781 gccaataata tttatctggc aactttctat tctgaaacac gcaaaatgac gccaataact
      841 ggcggctttg ccaataagac acagaacttt gaagcggtcg ctcaatacca gtttgacttt
      901 ggtctgcgtc catcgctggg ttatgtctta tcgaaaggga aagatattga aggtatcggt
      961 gatgaagatc tggtcaatta tatcgacgtc ggtgctacgt attatttcaa caaaaatatg
     1021 tcagcgtttg ttgattataa aatcaaccaa ctggatagcg ataacaaatt gaatattaat
     1081 aatgatgata ttgtcgcggt tggcatgacc tatcagtttt aatggatatt gccggatgtg
     1141 atacatccgg cacatttcac tcacgccgtt aacttcaccg gttcatcacg aaaatcatcc
     1201 gccggttcca gcttcagatt cagcgtcgtc agcagttcac gcagcttctc gtgaaactca
     1261 cgtagggtgt gctccagtcg ttcaaccact tcagtgtctt ttaccggaac actcttccag
     1321 tcgcctgctt tatcgaacag accaaactgg taactgtagg taaaacggga ttcctgcgct
     1381 tcaagctcca tccaccagcc ccagaactca cgcacttccg gtgccggttt cacgttgacg
     1441 catacagcca gacaatcgaa aaagaatcga ttatcttcgc acttaccttc acgaatatac
     1501 gggcctagtg cggtaaattt tttgatcaat ctgctcttcg ggtgtccact cggtaacgtc
     1561 attgcaatct cctgttgtga tgcaactgtt ttaccaaatt ggcaaatttt agcaaatttt
     1621 taacacaagc gtttttcgat ccagccggtg atttcctgaa gacctttgtc aaaattccga
     1681 tacaccgggt taaatgggat ctctaataat ttaccgtcag cagatgatga ggtgattaag
     1741 cgtgagtcct cttccgggct gaacggatcg ttcttccagt agcctgataa cattggcgtt
     1801 gggcagcgac gccccagcaa tccttgcact tttaatgaat agcgattcag ctccacgcgt
     1861 aacgcttcgt cggaagcatc atgcatcccc aaacgactcg ccagaacgtc aagatacatt
     1921 tccggcacct gttgctggca cttaaaatca ctcaacaggg tatgaactac cggaccaaga
     1981 caggcaaccg ctttcagacg cggcgattca agatatgcca gacgcacggc aacgttagcg
     2041 ccgaaacgga aaccaaaggc cgcgacgcga gtgtgatcca cccacggtac gttaggcaga
     2101 gcctttaaaa cgtgctgatg caacaggctg gagtcctggg tgagcttcca ttttgatgaa
     2161 aagcccaccg acggcatatc aatagtcagc atcgcaatgc cgcgcggcgc aaaataacgt
     2221 tcatacaggc tgtaatagtc cgtctgcatc gcatccagac caccacacat taatactgtc
     2281 gggaacgggc catcgccttt cggcatatgc aaaaagccgg tgatgggcgc accgccgggt
     2341 acggtaaact ccatctgccg catcgtgccc ggcagacgct gggcggcttc ttcataggca
     2401 cggtttgaca aagcctgcgc ttgctcggcc aggtcatctc ctttcagatg aggataggca
     2461 gcaatgttgt acaacgtagc cgcatgcagc cagtgacgac cactgagttg cggatcttct
     2521 tccgcacagg ctttttgttg ccacaccatc gcctggatgg cccactcata aatccagttg
     2581 ccaccacggt agccaatcac cgtatcgtat aaatcatcgt cggtacgttc ggcatcgctc
     2641 atcacaatac gcgcctggac gtcgaggatt tcacgtgggt caatgccgcg ccagatccac
     2701 atcagacggt taatcatgcg ataccagtga gggatggttt taccatcaag ggccgactgc
     2761 acaggcggtt gtgcgccgtg attaaagcgg cggactagcg tcgaggtttc aggatgttta
     2821 aagcggggtt tgaacagggt ttcgctcagg tttgcctgtg tcatggatgc agcctccaga
     2881 atacttactg gaaactattg taacccgcct gaagttaaaa agaacaacgc ccggcagtgc
     2941 caggcgttga aaagattagc gaccggagat tggcgggacg aatacgacgc ccatatccca
     3001 cggctgttca atccaggtat cttgcgggat atcaacaaca tagtcatcaa ccagcggacg
     3061 accagccggt tttgcgaaga tggtgacaaa gtgcgctttt ggatacattt cacgaatcgc
     3121 aaccgcagta ccaccggtat ccaccaggtc atcaataacg atgaagcctt cgccatcgcc
     3181 ttctgcgcgt tttagcactt taagctcgcg ctggttgtcg tgatcgtagc tggaaataca
     3241 aacggtatcg acatgacgaa tacccagttc acgcgccagt aacgcacccg gtaccagacc
     3301 gccacggctt acggcaataa tgcctttcca ttgttcagaa ggcatcagtc ggcttgcgag
     3361 tttacgtgca tggatctgca acatgtccca ggtgacgatg tatttttcgc tcatgtgaag
     3421 tgtcccagcc tgtttatcta cggcttaaaa agtgttcgag gggaaaatag gttgcgcgag
     3481 attatagaga tctggcgcac taaaaaccag tatttcacat gagtccgcgt ctttttacgc
     3541 actgcctctc cctgacgcgg gataaagtgg tattctcaaa catatctcgc aagcctgtct
     3601 tgtgttgaca acattttctg ctaaccctgt gacctgcaat actgttttgc gggtgatcga
     3661 caaggagact taacgtgtct gaactgtctc aattatctcc acagccgctg tgggatattt
     3721 ttgccaaaat ctgttctatt cctcacccgt cctatcatga agagcaactc gctgaataca
     3781 ttgttggttg ggcaaaagag aaaggtttcc atgtcgaacg cgatcaggta ggtaatatcc
     3841 tgattcgtaa acctgctacc gcaggtatgg aaaatcgtaa atcggtcgtc ttacaggccc
     3901 acctcgatat ggtgccgcag aaaaataacg acaccgtgca tgacttcacg aaagatccta
     3961 tccagcctta tattgatggc gaatgggtta aagcgcgcgg caccacgctg ggtgcagata
     4021 acggcattgg tatggcgtct gcgctggcgg ttctggctga cgaaaacgtg gttcacggcc
     4081 cgctggaagt gctgctgacc atgaccgaag aagccggtat ggacggtgcg ttcggcttac
     4141 agagcaactg gttgcaggct gatattctga ttaacaccga ctccgaagaa gaaggtgaaa
     4201 tctacatggg ttgtgcgggg ggtatcgact tcacctccaa cctgcattta gatcgtgaag
     4261 cggttccagc tggttttgaa accttcaagt taaccttaaa aggtctgaaa gggggtcact
     4321 ccggcgggga aatccacgtt ggcctgggta atgccaacaa actgctggtg cgcttcctag
     4381 cgggtcatgc agaagaactg gatctgcgcc ttatcgattt caacggcggc acactgcgta
     4441 acgccatccc gcgtgaagcc tttgcgacca ttgctgtcgc agctgataaa gtcgacgtcc
     4501 tgaaatctct ggtgaatacc tatcaggaga tcctgaaaaa cgagctggca gagaaagaga
     4561 aaaatctggc cttgttgctg gactctgtag cgaacgataa agctgccctg attgcgaaat
     4621 ctcgcgatac ctttattcgt ctgctgaacg ccaccccgaa cggtgtgatc cgcaactcag
     4681 acgtggcaaa aggtgtggtc gaaacctccc tgaacgtcgg tgtggtgacc atgactgata
     4741 ataacgtaga aattcactgt ctgatccgtt ctctgatcga cagcggtaaa gactacgtgg
     4801 tgagcatgct ggattcgctg ggtaaactgg ctggcgcgaa aaccgaagcg aaaggcgcat
     4861 atcctggctg gcagccggac gctaattctc cggtgatgca tctggtacgt gaaacctatc
     4921 agcgtctgtt caacaaaacg ccgaacatcc agattatcca cgcgggcctg gaatgtggtc
     4981 tgttcaaaaa accgtatccg gaaatggaca tggtttctat cgggccaact atcaccggtc
     5041 cacactctcc ggatgagcaa gttcacatcg aaagcgtagg tcattactgg acactgctga
     5101 ctgaactgct gaaagaaatt ccggcgaagt aattatttga tttgctgccg gatggcgttt
     5161 aatcgccttc cggcagtttc atccttcatt atccttcgat aaaagccatc cctgtaaatg
     5221 tccgtctcgg gttgccacgt tcaatctggt gatggaacat tcgccgctgc gatttcagca
     5281 ccgcgctatt ttcctgttgc tgctgctcca gcttccaggc aatcagcaat cgtgccagcc
     5341 gcttgttagc atgctgacta cgctctgact gaaccttcac gctaataccg gatgccaaat
     5401 gcgtggcgcg taccgccgag tcggttttat tgacatgttg accgcccggc cccgacgaac
     5461 gcaacgtctc ataacggatt gcatccgatt gttcctgctc atcagcggta aaacgcccaa
     5521 tgcccagaaa ccagtttttg cgcccatgat gaggccgata cggactcgga caaatccact
     5581 gaatagtgcc gcaccagctt tcgcttaatg cccatgcgtt atcgccatcc agagaaacca
     5641 gagctgaacg cagcgtgtca gagtagcggc ccgtttctgt ttccaacacc gttaccgcga
     5701 cgtcttgtcg ggcagcttct ttaattaatc gctccagcgc ctttttcact gcgagacaac
     5761 attcttccgg cccctgcgct gaggagagtt gtagcaagat catccttttt tccctccact
     5821 gtttttgagc gttagcaccg gacgcagccg cgcgacagga ataattaaac cagcctgcac
     5881 cagacattgc accacgcttt cagccgattt ataggcttgt ggcgcttctt caaagatgag
     5941 ttgtttatcg cgacaaatta cccgactgcc aagttcagta cgtgagagct gcgtcgctgt
     6001 gtatttcgct gccagacgcc ctttacactc ggtgcgcccc catttacgcc cagccccatg
     6061 cgccagcgag tggagcgttt tttcgttcgc aacaggctgt acaagctagg agtaatcacc
     6121 gagtgaaccg ggaataatca ccagaccgtt gtcatccggc gtggcacctt tacgatgcaa
     6181 ccagccctgt tgatcgccga tttgacacgc gctaacaaag ttatgcgcca catccagaac
     6241 cggacttccg gtggccttaa cctgttgcat tatgcgtaaa gagatcagct ggcgattaat
     6301 acgcgcaaac gccagcgcat cgtcatgttc cgcaatatac cgtagcgcgt cgtcactgcc
     6361 ttcaggcaaa ccatgatgcg aaaacgaggc aatatgccgc tgtaaaatag ac
//
LOCUS       DAZOZY010000213         6360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZOZY010000213 DAZOZY010000000
VERSION     DAZOZY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.215"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="ACHURQ_003663"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="ACHURQ_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR0825346.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="ACHURQ_003664"
     CDS             2219..2767
                     /locus_tag="ACHURQ_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR0825347.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="ACHURQ_003665"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="ACHURQ_003665"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR0825348.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHURQ_003666"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="ACHURQ_003666"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR0825349.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHURQ_003667"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="ACHURQ_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR0825350.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1599 a         1561 c         1612 g         1588 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gcgctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcg tcaataagtt
     6181 ctgtcaataa aaatttacgg aactattgat gagagtttgg tgtctttatg tgtctgcagg
     6241 catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt gctacaaaga taaagattta
     6301 gtttcaaaaa gaaaaaatat gtaaccaaaa gtaaaaatta aggaactttg tgaacaccgt
//
LOCUS       DAZOZY010000214         6318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZOZY010000214 DAZOZY010000000
VERSION     DAZOZY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.216"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..411
                     /locus_tag="ACHURQ_003668"
     CDS             <1..411
                     /locus_tag="ACHURQ_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0825351.1"
                     /translation="VGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLA
                     YTQRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVD
                     WYNNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            457..807
                     /gene="traA"
                     /locus_tag="ACHURQ_003669"
                     /pseudo
     CDS             457..807
                     /gene="traA"
                     /locus_tag="ACHURQ_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            822..1133
                     /gene="traL"
                     /locus_tag="ACHURQ_003670"
     CDS             822..1133
                     /gene="traL"
                     /locus_tag="ACHURQ_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR0825352.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            1155..1337
                     /locus_tag="ACHURQ_003671"
                     /pseudo
     CDS             1155..1337
                     /locus_tag="ACHURQ_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            1339..2589
                     /locus_tag="ACHURQ_003672"
                     /pseudo
     CDS             1339..2589
                     /locus_tag="ACHURQ_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2586..2972
                     /gene="trbI"
                     /locus_tag="ACHURQ_003673"
     CDS             2586..2972
                     /gene="trbI"
                     /locus_tag="ACHURQ_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR0825353.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2969..3280
                     /locus_tag="ACHURQ_003674"
                     /pseudo
     CDS             2969..3280
                     /locus_tag="ACHURQ_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            3275..4273
                     /locus_tag="ACHURQ_003675"
                     /pseudo
     CDS             3275..4273
                     /locus_tag="ACHURQ_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            4293..5040
                     /gene="traX"
                     /locus_tag="ACHURQ_003676"
                     /pseudo
     CDS             4293..5040
                     /gene="traX"
                     /locus_tag="ACHURQ_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            5095..5655
                     /locus_tag="ACHURQ_003677"
     CDS             5095..5655
                     /locus_tag="ACHURQ_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR0825354.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5785..5997
                     /locus_tag="ACHURQ_003678"
     CDS             5785..5997
                     /locus_tag="ACHURQ_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR0825355.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1502 a         1531 c         1927 g         1358 t
ORIGIN      
        1 gtggggtggc gggtctcatc gtccatggag acgacattcg tgctggatgc actggagcag
       61 gcgttatggg cccgtcgacc gtccggcacg gtccatcaca gtgataaagg ttctcagtat
      121 gtatcgctgg cctacacaca gcggcttaag gaagccggat tactggcatc aacaggaagt
      181 acaggcgact cgtatgacaa cgcgatggcg gagagcatca atggtcttta caaagcggag
      241 gtaatacacc gtaagagctg gaaaaaccgt gcagaagtgg aactggccac actcacgtgg
      301 gtggactggt ataacaatcg acgattgctg gaaaggctgg gccatactcc tccggcagaa
      361 gcagaaaaag cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga
      421 taaaacactc tccaggaaac ccggggcggt tcagcgttaa gtgttcaggg tacttctgcg
      481 cccgtcaaaa agaaatcgtt tttttctaaa ttcactcgtc tgaatatgct tcgcctggtt
      541 cgtgcagtga tcccggttgc tgttctgatg atgttgttcc ctgagttagc tatggctgct
      601 ggtaaaggca cggacctgat ggctaaaggt caggaaacgg ttaaggcgac cttcggtaag
      661 gactccagta tcgttaaatg ggttgttctg gctgaagttc tggtcggtgc agtcatgtac
      721 atgatgacca aaaacgtcaa gttcctggtc ggctttgcca tactctctgt atttattgca
      781 gtaggtatgt ctgttgccgg attctgacag gaaataaaac gatgttggga gacgagaata
      841 aacttaagaa atatcgtttc ccggaaacac tgaccaacca gagccgctgg tttggcctgc
      901 cactggatga actgatcccc gcagcaatct gtgttggctg gggtatcaca acatcgaaat
      961 atctgttcgg tattggtgca gcggttctgg tttatttcgg gattaaaaaa ctgaaaaaag
     1021 ggcggggcag ttcctggtta cgtgacctga tttactggta tatgccaaca gccctgctgc
     1081 gcggtatttt tcataatgtt cccgattcgt gtttccggca gtggattaaa tagaactgat
     1141 accaggattg ttatatggaa cacggtgccc gtttaagtac cagtcgtgta atggccatcg
     1201 cctttatatt tatgtcagtg cttattgttc tcagcctctc tgttaacctc attcaggggg
     1261 tgaataacta ccgtcttcag aatgagcaac gcactgccgt gaccccaatg ggatttaatg
     1321 tgccctttgc cgtgtcacac ctaccgtaac cagctggcgt ttatcgatat tttcttccgg
     1381 ggaatgaata acaccaacta caacatggcg gtctgtggca cctccggggc cggtaaaacc
     1441 gggctgatac agccacttat ccgcagcgtg ctggactccg ggggctttgc cgtggtgttc
     1501 gacatggggg atggctacaa gtccctgtgt gagaacatgg ggggcgtata tctggacggt
     1561 gaaaccctgc gttttaaccc gtttgcgaac atcaccgata tcgaccagtc agcggagcgt
     1621 gtccgtgacc agttgtcagt gatggccagc cccaacggta acctggatga agtgcatgaa
     1681 ggtctgctgc tgcaggcggt cagggcctcc tggctggcca aagagaacag ggcacgtatt
     1741 gatgacgtgg tggatttcct gaaaaacgcc agtgacagcg agcagtatgc cgggtcaccg
     1801 actatccgca gccgtctgga cgaaatgatt gtgctgcttg accagtacac tgccaacggc
     1861 acttacggcc agtattttaa ctctgatgag ccgtccctgc gggatgacgc aaaaatggtg
     1921 gtgctggagc tgggcggact ggaagaccgt ccgtcactgc tggttgcggt gatgttctcc
     1981 ctgattatct acatcgagaa caggatgtac cgcacgccgc gtaacctcaa gaaactgaac
     2041 gtcattgatg aaggctggcg tctgctggac ttcaaaaatc acaaggtcgg tgagtttatt
     2101 gagaaaggtt accgtaccgc ccgtcgtcat accggtgctt atatcaccat cacacagaac
     2161 atcgtcgact ttgactctga caaggcctcc agtgcggccc gtgcggcatg gggtaactcc
     2221 tcctacaaaa ttatcctcaa acagagtgcg aaggagttcg caaaatacaa ccagctgtat
     2281 ccggaccagt tccagccact gcagcgcgac atgattggta agtttggtgc ggccaaagac
     2341 cagtggttca gttccttcct gctgcaggtg gaaaaccatt cctcctggca ccgcctgttt
     2401 gtggacccgt taagccgcgc catgtacagc tctgacggcc cggattttga gtttgtgcag
     2461 cagaaacgta aagaggggct gagtattcat gaggcggtgt ggcagctggc gtggaagaag
     2521 tcagggccgg agatggcttc gctggaagcc tggctggaag aacatgagaa atacaggagt
     2581 gttgcatgac cacaacgcaa aaaacgactg atgttacagc cccccgacgc agccactggt
     2641 ggtggacggt gccgggatgc ctggcaatgg tgttactgaa cgcagccgtc agttacggca
     2701 ttgtcagact gaacgcaccg atgactgccg ccttcaacat gaaacagact gtggatgcat
     2761 tttttgacag cgccagtcag aaacaactgt cagaagcaca gtcaaaagcc ctttcagcgc
     2821 gttttaacac ggcactggaa gccagcctgc aggcgtggca gcagaaacac cacgcggtca
     2881 ttctggtgtc gcctgccgtg gtacaggggg ctccggatat cacccgggaa atccagcagg
     2941 atattgcccg gcgaatgaga gcggaaccat gagatgccgg gggctgattg ccctgctgat
     3001 atgggggcag agtgtggccg ccgccgatct tggcacctgg ggtgatctgt ggccggtaaa
     3061 ggagccggac atgctgacgg tgatcatgca gcgcctgacg gcactggagc agtccggtga
     3121 gatgggccgg aaaatggatg cctttaagga gcgggtgatc cgcaacagtc tgcggccccc
     3181 tgccgtgccc ggtatcggac gcacggagaa atacagcagc cggttgtttg acccgtccgt
     3241 cagactggct gcggatatcc gggataacga agggcgggtg gccggctttg agtcagccta
     3301 cgtggccctg tcgcgtatga agcagcatgt gcaggtgtac accgataacc gtcagggctg
     3361 gacggatgcc atcaacaatg ccgtacagaa aggaactgcc cacgatgtgt ttgagccgaa
     3421 accggaccgg gaggtcatga atgcagagcg gctgttcagt acggcgcggg agctgcggga
     3481 cgtggcggca ggccgtgccg ttctccgtca ggcggggctg gccgggggag acagtcctgc
     3541 acggtttatt gctccgggac gtaaatatcc gcaaccgtat gtggcactgc cggcgtttga
     3601 ccgtaacggc aagtccgccg gtatctggct gaacccgctg accacggatg acggaaacgg
     3661 gctgcgggga ttcagcggtg aaggtcgggt aaaaggcagc ggggatgcgc agttcgtggc
     3721 cctgcagggc agccgtaacg gagaaagcct gctggctgat aatatgcagg atggcgtccg
     3781 gattgcccgt gataatcctg acagtggtgt ggtggtaagc attgccggtg aaggccgtcc
     3841 gtggaatccc ggtgccatca ccgatggccg cgtgtggggg gatatcccgg acaacagcgt
     3901 ccagccggga gccggaaatg gcgaaccggt cacggcagag gtactggcac agcggcaggc
     3961 tgaagaggcc atccgccgtg aaacggaacg tcgcgcagat gaaattgtcc gtaaaatggc
     4021 agagaacaaa cctgacctgc cggacggcaa aacagagctg gctgtcaggg aaattgccgg
     4081 gcaggagcgt gaccgggctg ccataactga acgggaagcc gcgctgccgg agagtgtgct
     4141 gcgtgaatca caacgggagc gggaagcggt ccgggaggtt gcccgggaaa atctgctgca
     4201 ggagcgtctg cagcagatgg agcgggatat ggttcgtgac ctgcagaaag agaaaacccc
     4261 gggcggagac tgatacagga agacaaacac tgatgacaac ggataacacg aacacgacac
     4321 gtaacgattc actggctgca cggaccgata cctggttgca gtcatttctg gtctggtcac
     4381 ccggacagcg ggatatcatc aaaacggtgg cactggtgct gatggtactg gaccacatca
     4441 atctgatatt ccagctgaag caggaatgga tgtttctggc cgggcgagga gcctttccgc
     4501 tttttgccct ggtgtggggg ctgaatctgt cccgtcatgc gcatatccgg caaccggcca
     4561 ttaaccggct gtggggatgg ggaattattg cccagttcgc atattatctg gcaggctttc
     4621 cctggtatga ggggaatatc ctgtttgcct ttgcagtggc ggcacaggtg ctgacgtggt
     4681 gtgaaacgcg cagtgggtgg cgtacagcag cggccattct tctgatggcc ctgttggggg
     4741 cctttgtccg gcaccagtta cggcattgcc gggctgctga tgctggcagt cagccaccga
     4801 ctgtaccggg cggaagacag agcggaacgt ctggcgctgg tcgcctgcct gctggctgtt
     4861 attccggcgc ttaaccttgc caccagtgat gcagcggcgg tggccggtct tgtgatgacg
     4921 gtgctgaccg ttggtctggt gtcgtgtgca ggaaaatcat taccccgttt ctggcccggg
     4981 gattttttcc cggtattcta cgcctgtcat ctggctgtgc tgggcgttct ggcgctgtga
     5041 cgggtgtggt atctttgacc gtaacaggat gattcgtcag aggcagaaca cagcatgaca
     5101 gagcagaagc gaccggtact gacactgaag cggaaaacgg aaggggagac gcctgtccgc
     5161 agccggaaaa ccatcatcaa tgtcaccacg ccaccaaagt ggaaggtgaa aaagcagaag
     5221 ctggccgaga aagcagcccg ggaagcagag ctggcggcaa aaaaagcgca ggccagacag
     5281 gcactgtcca tttatctgaa cctgcccacg ctggatgagg ccgtgaatac cctgaaaccc
     5341 tggtggccgg gattatttga cggtgacacc ccccggcttc tggcctgcgg tatccgggac
     5401 gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa actgcgcagg
     5461 gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg ccatgaaagc cggtgcctgc
     5521 cggtatgaca cggaagggta tgtgacggag catatttctc aggaggagga agcgtatgcg
     5581 gcagcgcgtc tggataaaat ccgccgccag aaccggataa aggcagaact tcaggccgtg
     5641 cttgatgaga aataaaaaaa agcctccatc agagaggagg cagggaaata aggcttaaaa
     5701 ggaaataact tccgcttccg aaaggaagcc agtccactga attatttatc acaataatac
     5761 tgacaactaa agggggacaa gaaaatgaat ggattcagaa acagttcacg gaacggccag
     5821 gtctggcgtt accagcgtgc cggcgggcgg gccgttattc tggaagtcag tggacgctgg
     5881 atggaagcgg cagaagcatg gcgacgggct gcctgtgttg cgcccaggac agactggcag
     5941 cagtttgccc gaaaaagggc tgaacactgc caccggcgct gccggggaag ggtgtaacga
     6001 aaaaagcagc cttcattact ccaggagtca gaaggctgaa aggagtttat gagatttgct
     6061 ttttttaata tccggtcggg cagtatgacc ggacagttct gagtagcaca gagttttttc
     6121 cgtattaagt cgtgatttta tattttgtga ttaatttcac aaaataaggt gttattcagt
     6181 gtgtgctgca atattcagga tgcctgaaat acagatgcta tttatctgat atggagaata
     6241 acatgaggaa atagtaaggc agtatgactt tccgtgagac agatgctggc caacgtggga
     6301 tatttaacgc gggttaac
//
LOCUS       DAZOZY010000215         6286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZOZY010000215 DAZOZY010000000
VERSION     DAZOZY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.217"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..356
                     /gene="mcbR"
                     /locus_tag="ACHURQ_003679"
     CDS             <1..356
                     /gene="mcbR"
                     /locus_tag="ACHURQ_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0825356.1"
                     /translation="TPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNM
                     PILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAE
                     IMQQNIAILYQQYNR"
     gene            complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHURQ_003680"
     CDS             complement(392..2494)
                     /gene="pqqU"
                     /locus_tag="ACHURQ_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR0825357.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2736..3797
                     /locus_tag="ACHURQ_003681"
     CDS             2736..3797
                     /locus_tag="ACHURQ_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR0825358.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHURQ_003682"
     CDS             complement(3912..5411)
                     /gene="ansP"
                     /locus_tag="ACHURQ_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR0825359.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5678..>6286
                     /locus_tag="ACHURQ_003683"
     CDS             5678..>6286
                     /locus_tag="ACHURQ_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR0825360.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNE"
BASE COUNT         1693 a         1592 c         1504 g         1497 t
ORIGIN      
        1 tcaccccgca agacctgacg gaactccagg aattgctcga gaaattacag caggcgcaag
       61 aaaagggcga catggaacaa atcattaatg taaacaggct atttcgctta gcgatttatc
      121 atcgctcaaa tatgcccatc ctgtgtgaga tgattgagca actgtgggtc aggatggggc
      181 ctggtttaca ttatctctat gaagcgatta atccagcgga attacgggag catatagaaa
      241 actatcatct gttactcgct gcgttaaaag caaaagacaa agagggatgc agacattgtc
      301 ttgctgaaat tatgcaacaa aatattgcta ttttatacca gcaatacaat cgttaaagta
      361 aatgcccggc aaataccggg catcaaggta attactcaaa tctccacgca atattcatgc
      421 cgacaccata atttcgtccg ggcgaaggtt cgtaatatcg cccgtttgac tcattgacaa
      481 tgacagaacc aacgtattct ttatcgaata aattatcgac acgaccaaat aaatccacag
      541 ttaaattgtg gtaattgtat ttatacccgg tgaataagcc gacgagagta taagacggcg
      601 cttttgccgt attttcatca tctgccataa tatcgcccat ataacgcgct tccgtgcctg
      661 cataccaacc atcttccggt acataaccta tcgacgcaaa gcccatatta cgggcgatcc
      721 ctggcatccg attaccgtta caatcctgtt cattgcaaac attgctgcga taggtcgcat
      781 caagccaggt ccaggacgcg tttacgcgaa aatctcctgc gaaacgttga tcccatgcca
      841 gttcagcgcc ttgacgacgg gtctttccgg catttttgta agtcgtacgc ccaccgctac
      901 tgctatcgac aacaatttca tcatcagtgt cggtctgaaa caatgcgaga ctaagcagcc
      961 catcaccaat acgcgtttta ctgccgatct caattgtatc gttggtggat ggttttaaac
     1021 caaagttcat accgctttgc ccatcagcac gataagacag ctcattaatc gtcggcgttt
     1081 caaaacctcg cccggctgcc agatagatat tccaggcatc ggtcattgca tattttaacg
     1141 aaccggcagg tagccattta tgataactgg catcaccgct gtcatcgccg ttacccggag
     1201 taacgtaatg gtcgttggaa tcaaaccaca cggagctgta gcgcacgcca gcatccagcg
     1261 acagtttttc gctcagctgc cactgcgtct gtaaataggg atcgatgttc cacatcagat
     1321 tgcgttcgtc gcgacgcaac tcaccttttt gcccgtactc cggcatgccg ctattcaggc
     1381 ggaagttatt gtagcccttg cggttttcac tcatgttttc gtagttcagg ccggtagtga
     1441 acgtgaccgg aacgcccagt tcaccacggt gtgtccagcg gctgtctatc ccctggtaat
     1501 ggcgttgcag ggtaatcacg ccgcccgcat gtgacgggtt aagttgtggt gccatgggta
     1561 ttgactggta ctgggtcgtt tctcgctctc cggcatacat catcacactc atatcatctt
     1621 gcgcgctcag gctacgctca tagcgcaacc cagcctgagt ttgcttgatg gtttttcgcg
     1681 tgtcgtactg ttctgcacga ggcgcttgtt gtggattagc cttccattct gctttggtta
     1741 gcccacccgg gtcatctgct ttgatatcca cactattgaa aatcagactt aatttgctgg
     1801 cttcatcaat gcgtacgccc agtttggcat tggctaaatt tttctgtgcg ccactatggt
     1861 cacgatagcc gtgggtcgta aaacgcgtgg ttgagacggt gtaatcgaca tcgccaggct
     1921 gtgtgccgtc tcccgttgcg cccgttgctt tcagcccata gcgccagctg ccaaaactgc
     1981 cgtagtaact actggcttca atggttggtg gctgttgtcc ggtctgggtg gtgacattca
     2041 ttaccccacc agacgcgttg ccatacaggg cagagaaggg gccacgcagc acttccacat
     2101 tttgcacact gcttaaatcg atgttggatg tttgcccttg cccgtcgggc atggtggcgg
     2161 gaataccgtc cacatacagg cgaataccgc gaataccgta agtggagcgg gagccaaatc
     2221 cgcgaatcga cagctgtaaa tcttgcgcat agttctgccg gttttgtacc tgcaaaccag
     2281 gcacgtcggt cagtgattcg gacaagttaa tgcgcggtgt tgccaggcgc atctcctcgc
     2341 catccaccac gcttactgct gctgggatat ccagttctga aaccacctgc ggtgcggcac
     2401 tgacaatcat ggtctgttca tcagcggcaa aaacaacggg ggaaagggca agtagtgcgg
     2461 gcaaaacggt ctgtcggacg gaaaaaatct tcatgaaaaa agccaggtta agaatgggaa
     2521 aacgccgtta tggtaataaa attgtaaatt catggaaaac gaaacggcac gatacgttaa
     2581 gtaattgaga aaattgtagt cgtaacggca agaaatgctc cacatttgag aaaataatga
     2641 ttaccattcc catttataac aagagcgtaa cgatgattac gcttagcgaa gcattgtgaa
     2701 gcagcaaaaa tatcggttca tcaaagggag tcgtcatgca tttacgtcat ctgttttcat
     2761 cgcgcctgcg tggttcatta ctgttaggtt cattgcttgt tgcttcatca ttcagtacgc
     2821 aggccgcaga agaaatgctg cgtaaagcgg taggtaaagg tgcctacgaa atggcttata
     2881 gccagcaaga aaacgcgctg tggctcgcca cttcgcaaag ccgcaaactg gataaaggcg
     2941 gcgtggttta tcgtcttgat ccggtcactc tggaagtgac gcaggcgatc cataacgatc
     3001 tcaagccgtt tggtgtcacc atcaataaca cgactcagac actgtggttt ggtaacactg
     3061 taaacagcgc ggtcacggcg atagatgcca aaacgggcga ggtgaaaggc cgtctggtgc
     3121 tggatgatcg taagcgtacg gaagaggtgc gcccgctgca accgcgtgag ctggtagctg
     3181 acgatgccac gaacaccgtt tacatcagtg gtattggtaa agagagcgtg atttgggtcg
     3241 ttgatggcga gaatatcaaa ctgaaaaccg ccatccagaa caccggtaaa atgagtaccg
     3301 gtctggcgct tgatagcaaa ggcaaacgtc tttacaccac taacgctgac ggcgaattga
     3361 ttaccatcga caccgccgac aataaaatcc tcagccgtaa aaagctgctg gatgacggca
     3421 aagagcactt ctttatcaac attagccttg ataccgccag gcagcgtgca tttatcaccg
     3481 attctaaagc cgcagaagtg ttagtggtcg atacccgtaa tggcaatatt ctggcgaagg
     3541 ttgcagcacc ggaatcactg gctgtgctgt ttaacccagc gcgtaatgaa gcctacgtaa
     3601 cgcatcgtca ggcaggtaaa gtcagtgtga ttgacgcgaa aagctataaa gtagtgaaaa
     3661 cgttcgatac gccgactcat ccaaacagcc tggcgctgtc tgccgatggc aaaacgctgt
     3721 atgtcagtgt gaaacaaaaa tccactaaac agcaggaagc tacccagcca gacgatgtga
     3781 ttcgtattgc gctgtaatag atatggcgag ggtgaaaacg gagagacttg cctgactttt
     3841 atcacgccgc atgcggcaca ccttcgtcag taatctaaat ttgcccggca cgtagccggg
     3901 caattttgca attagctcgc cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct
     3961 tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac accaaaccag
     4021 cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt cccgttcggg
     4081 taatcgaacg ccatcagcac aaggacactc agtaaaaaca gtaatgtcag ccaggaagtg
     4141 aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttcttt aatcgcttta
     4201 cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc cagcgacgcg
     4261 aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa tacgccgacg
     4321 acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg actcattttc
     4381 gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg cccggtgcag
     4441 tacagacctg agttcaggct ggagagggca gcggtcagca ccacaatgtt cataatgctg
     4501 ccgatatatg gcatacccag tttagagaaa aacgtcacga acggactttg ccccgcctga
     4561 tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta aaacaggcca
     4621 atacgccaaa tcacactgtt aatggcttta ggcaccatgg tctgcggatc tttacattct
     4681 ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac
     4741 accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat taaatgaaag
     4801 ccagtggtgt tgccatccag cggctgacca ctaccgagga acactgtacc cacgaccaaa
     4861 aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc cgcaaaccat
     4921 ttcacaccga tcatattcat ggtgccaacg atggtaagtg cagcgagcgc aaagacccac
     4981 tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc ggtaatatca
     5041 acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata agcggctttc
     5101 tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg gtgtagcacc
     5161 agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa ataaaccagt
     5221 gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa gccggtgcca
     5281 atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt gtgatacccc
     5341 tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgtcg
     5401 tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt tgccatgaac
     5461 aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg tataaaacta
     5521 tcggcgcagc atcctacccg ttatcgatat acgatgcaaa acatcccctt acaatcctga
     5581 aggggattca tacaactgac gaaaaaatga caaatacttt tgctggttaa cctgtgtact
     5641 gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt atggcgtacc
     5701 cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc cttatcagtt
     5761 cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga aaaccctgaa
     5821 cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga cggagacagc
     5881 ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg ttgggcgcgc
     5941 cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct atccaacatt
     6001 cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt taaagaagaa
     6061 tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga ccgctgaacc
     6121 ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta tgcgcacatg
     6181 gggggctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga ttgctgatgc
     6241 tgtatgtcag ttaccgaaat tacaagaagt attaaaaagg aatgaa
//
LOCUS       DAZOZY010000216         6250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZOZY010000216 DAZOZY010000000
VERSION     DAZOZY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.218"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            45..1505
                     /locus_tag="ACHURQ_003684"
     CDS             45..1505
                     /locus_tag="ACHURQ_003684"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR0825361.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHURQ_003685"
     CDS             complement(1541..1744)
                     /gene="ydfZ"
                     /locus_tag="ACHURQ_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR0825362.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHURQ_003686"
                     /gene_synonym="ydfH"
     CDS             complement(1921..2607)
                     /gene="rspR"
                     /locus_tag="ACHURQ_003686"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR0825363.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHURQ_003687"
     CDS             complement(2696..3442)
                     /gene="ydfG"
                     /locus_tag="ACHURQ_003687"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR0825364.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3579..5624
                     /gene="dcp"
                     /locus_tag="ACHURQ_003688"
     CDS             3579..5624
                     /gene="dcp"
                     /locus_tag="ACHURQ_003688"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR0825365.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5669..6187)
                     /locus_tag="ACHURQ_003689"
     CDS             complement(5669..6187)
                     /locus_tag="ACHURQ_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR0825366.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT         1556 a         1491 c         1604 g         1599 t
ORIGIN      
        1 taacaaaaca tatagcttgc catatttata tttaaggaat tgttatggga aataatttgt
       61 tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca agaattattc
      121 atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat attcttgcta
      181 cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc gaacagcaaa
      241 ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg gccgatgcgt
      301 ggacggctcg cgtcgttggc gtcgttaaga aagccttgca cgtacagatg gatggcttag
      361 aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg acaatcaccg
      421 aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac ccgatggtcg
      481 ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt gttgaggcgc
      541 tggcccgccg taaagcggca ggacttcccg catttaccgt catgtcatgt gacaacatgc
      601 cagaaaacgg tcatgttatg cgtgacgttg tcacttcata cgcgaaagct gttgatgtaa
      661 aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg gaccgtattg
      721 tgcccgcagt gacagaggat acgctggcga aaatcgaaca acttaccggt gtgggcgatc
      781 ctgcgggcgt tgcctgtgaa cctttccgcc agtgggtaat agaagataac tttgttgccg
      841 gacgtccaga atgggaaaaa gcgggagccg aactggttag tgatgtgctg ccttatgaag
      901 agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcgtatttg ggttatcttg
      961 ctggatatca gcacattaat gactgcatgg aagatgaaca ttatcgttat gcggcgtatg
     1021 gcttgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt
     1081 acgctaaccg attaattgaa cgctatagca acccggcgtt acgtcatcga acctggcaga
     1141 ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc
     1201 tggcgcatga cagcaagttc gatctgctgg cactgggcgt cgcgggttgg atgcgttatg
     1261 tcggtggtgt tgatgaacag ggaaatccga ttgaaatcag tgacccactg ttacctgtta
     1321 ttcagaaggc tgtacaaagt agtgctgaag ggaaagcgcg cgtccagtca ttgctggcga
     1381 ttaaggcgat ctttggtgat gatttgccag acaatagttt gtttactgca aaagtgacgg
     1441 aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa tattccgtga
     1501 agtaaaaaaa gcaatgccgc catcggcggc attcacacgc ttagttcatg ccgagacgaa
     1561 tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt tttccgcggc
     1621 ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca gtgtgaccgg
     1681 tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga tcgtaggtcg
     1741 tcataggtat tttctccttt ctgatttaca gtcgaagccc acgtaacggg cgcaatataa
     1801 atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt ttttgataca
     1861 tgcatgagcc ataaggcggg ggaaaagact ctcaccccgg atgggatgag aggaaatgaa
     1921 ttactcttcg ctaaaccagt cgctattttc ctggcggatc tggcgcacgg attcgctgat
     1981 ttcctgcaaa tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc
     2041 agagaaaata tcaagatgct ggcgtaacag catttctggt ggagaaacgt ggtcgaaact
     2101 catatagcgc acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg
     2161 acagtcggca atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc
     2221 cagttgcttg cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca
     2281 ctggctttcg gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg
     2341 gataaaactg ccattgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg
     2401 cggacgaatt tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg
     2461 gcgtgacaca ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggtgcaat
     2521 caggcaatgg acaatgtcgc gacgaagaat acgataaatt tgctgattga caggctgtgt
     2581 gggattaagt tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga
     2641 gtctaccact tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact
     2701 gacggtggac attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga
     2761 tattgacgtg agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg
     2821 tcaatgcaac ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga
     2881 cattggaaaa ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg
     2941 taccatgcag gtccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac
     3001 cgtaaacgtt accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat
     3061 gaccatgatt acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt
     3121 tgttgttggt atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca
     3181 tgcccaacgc caggccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa
     3241 gcgatgccag catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca
     3301 gattatctcc cagttcgtct tttaactcct gcaaccgctc ctggcgacgg ccagtggcga
     3361 taactttatg cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg
     3421 ctccagttac taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata
     3481 gcacgtgatg cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa
     3541 gcctacgcta tggaacatct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc
     3601 ttgtgcaaag cacactaccg tatctggctc cccattttga tcaaattgcc aatcatcact
     3661 atcgcccggc attcgatgag ggaatgcagc aaaagcgggc agaaattgct gccatcgcgc
     3721 ttaacccgca aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat
     3781 tacttacccg cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat
     3841 tacagcgtct tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc
     3901 tgaacggtga attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc
     3961 ttgatagtga atccatccgc ctggtggagg tgattcatca acgttttgtc cttgccggag
     4021 ccaaacttgc gcaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc
     4081 tgaccagcca gtttaaccag cgattactgg cagcaaataa atccggcggt ctggttgtga
     4141 acgatatcgc gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg
     4201 ctcgcgagaa aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac
     4261 cggcgcttgc cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga
     4321 cgcgagcgga aaaaaatgat ggcaatgata cccgcgctat cattcaacgt ctggtagaga
     4381 ttcgcgcaca gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg
     4441 atcagatggc aaaaacgcca gaagcagcac tcaactttat gcgggaaatt gttccagcgg
     4501 cgcgtcaacg tgcgagcgat gaattagcct ccatacaggc ggttatcgat aagcagcagg
     4561 gcgggtttag cgcgcagccg tgggactggg cattttatgc ggaacaggta cggcgggaga
     4621 aatttgatct tgatgaagcg cagctcaagc catattttga attaaacacg gtgttgaatg
     4681 aaggtgtatt ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata
     4741 ttcctgtcta ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc
     4801 tggcgttatt ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg
     4861 gcaattttgt tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtctgca
     4921 attatcagaa acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtaataacct
     4981 tattccatga atttggtcat acgctgcacg gcctttttgc ccgccagcgt tatgccacgc
     5041 tttccggcac caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact
     5101 gggcaacgca tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa
     5161 tgcctgacga actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga
     5221 tgagcgaact gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg
     5281 aagcaatgca ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc
     5341 ttcctgctat accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat
     5401 atgctgcagg ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt
     5461 ggtttgttga gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc
     5521 tttccagagg taacagcgaa gatctggaac gcctgcatcg acaatggcgc ggtaaggctc
     5581 ctcagattat gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg
     5641 gcctggggat atcgccaggc cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg
     5701 caaacctcac cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag
     5761 tgtagttctg gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt
     5821 caccgccctc cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct
     5881 ctatggcacc tgttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa
     5941 gagactgctg gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc
     6001 ctgcaccgta aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc
     6061 cggtgcatga ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg
     6121 cggatgtcag gttttcaata gtgtcggtat ctgcgagttg tacaatttta tctttattaa
     6181 tgttcactgt tttttgtcca cgctattata tgtaaaggtg atttgctttt tctgtatgct
     6241 cgtccaatat
//
LOCUS       DAZOZY010000217         6215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZOZY010000217 DAZOZY010000000
VERSION     DAZOZY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.219"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(10..165)
                     /locus_tag="ACHURQ_003690"
     CDS             complement(10..165)
                     /locus_tag="ACHURQ_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0825367.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="ACHURQ_003691"
     CDS             complement(368..775)
                     /locus_tag="ACHURQ_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0825368.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="ACHURQ_003692"
     CDS             852..1079
                     /locus_tag="ACHURQ_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR0825369.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="ACHURQ_003693"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="ACHURQ_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1562..2361
                     /locus_tag="ACHURQ_003694"
                     /pseudo
     CDS             1562..2361
                     /locus_tag="ACHURQ_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2368..3114
                     /locus_tag="ACHURQ_003695"
     CDS             2368..3114
                     /locus_tag="ACHURQ_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0825370.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            3129..3551
                     /locus_tag="ACHURQ_003696"
     CDS             3129..3551
                     /locus_tag="ACHURQ_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0825371.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            3609..3965
                     /locus_tag="ACHURQ_003697"
     CDS             3609..3965
                     /locus_tag="ACHURQ_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825372.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            4134..4799
                     /locus_tag="ACHURQ_003698"
     CDS             4134..4799
                     /locus_tag="ACHURQ_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR0825373.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            5027..5182
                     /gene="hokD"
                     /locus_tag="ACHURQ_003699"
     CDS             5027..5182
                     /gene="hokD"
                     /locus_tag="ACHURQ_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0825374.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            5432..5719
                     /locus_tag="ACHURQ_003700"
     CDS             5432..5719
                     /locus_tag="ACHURQ_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825375.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            5758..>6215
                     /locus_tag="ACHURQ_003701"
     CDS             5758..>6215
                     /locus_tag="ACHURQ_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0825376.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
BASE COUNT         1644 a         1349 c         1690 g         1532 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacgatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag aggaagagga agatctaaat actgatctcc ccctcgccaa
     2041 aaacgagcgt ctaaaaaatt cgagccggag gctattgagc tgcccgattg gttgccggaa
     2101 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
     2161 cagggcgcta acggggcgat acgggaactg gaaaaattcc gtcagcaggg ttttacacct
     2221 gagcaggtga ttcgacacag catcgccaat gaataccagg gcctgttcgc gccgaaaggt
     2281 gttcggcctg agacgttgct ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc
     2341 ccgccaggct tcagggggta acggaccatg aaaaatattg cgacaggcgg cgttctggaa
     2401 cgcatccgcc gactgacccc gccacatgta accgccccat tcagaacggt agcggagtgg
     2461 cgcgagtggc aacttgctga aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc
     2521 cagttgcggg tggaaaaaat tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc
     2581 tcgtttgcga attaccaggt gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa
     2641 tccatcgccg atgaactgat gaccgggtgt acaaattttg cgttcagcgg aaaacttggt
     2701 accgggaaga atcacttagc ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca
     2761 gtgattgtgg ttaccgtggc tgatgttatg agcgccctgc acgccagcta tgacgacggg
     2821 cagtcaggcg aaaaattttt gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa
     2881 attggcattc agcgcgagac gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc
     2941 cggacagcgt cgatgcgcag cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa
     3001 acattgctcg gcgagcgggt gatggatcgc atggtcatga acggcgggcg ctgggtgaat
     3061 tttaactggg agagctggcg tccgaatgtt agccattcga gggttgttaa gtagtttcag
     3121 gaggatttat ggcgaaacct tttactcccg aacagcggga agaactgaag acgcgaattg
     3181 tggaactcgt gcatcaggac ggtcgggtca cgattcggca gttgtcagat gaaacaggta
     3241 tcagtcgtgc gtctgtcggt cgcttatgca tagaactggt cgcaagtggt gatgtatata
     3301 attctggcta cggcttattc ccgtctgaac aggctcgcaa ggactggcaa agcgcccgca
     3361 aaaaactctc gagagtaaag gtgaggaaac cggttgttgt tgatccggac cttatctggt
     3421 cattacctga cggagaaata cgccgctacg acaggcgcct gaatataatc tgtcgcgagt
     3481 gccggaagag cgaagctatg cagcgtgtac tggctttcta tcagggtaat tttcaggagg
     3541 cgatactgtg aatgaaatta gctatcaggc ttcaattacc gctggcattc gcatcaaagg
     3601 agaggagcat ggaaataaaa ccagaagatg agttaagcaa tatcgtttta tttccggtaa
     3661 aagaggatga ccctcgtaat caggttaatt ttctttatga gccatcggaa agaccatatt
     3721 gccatcacgc ctctgtacgg gttgacgaaa aagagcgtca ggtccgctgt aaaatctgcg
     3781 gtgcggttgt ggagccgttt gactggatgc tctctgtggc aaaaagagaa accagactgg
     3841 cagatgatgt aaggctattg cgccaggagg aacaggaaag gcggagaaat atagaaaagc
     3901 tgatacagat tgagcgtaac gcgaaagcgc ggatacgcag ggcgacaaaa tccagaactg
     3961 aataattaaa tttagcactg ttaaaaattt aatccttaac cggagggatt tctgcaccct
     4021 caaatcatca ggagaccacc cgaaagggcg gggagcagtc acacatctgt ttccgatagc
     4081 cccgttctaa tgctacactc tttgatattt ttatgacccc aataaacata tttatgacag
     4141 ttgctgattt caaacggccc aaattggagc tcccaaacgg ggcaaacaaa ctactactgc
     4201 actcttgctg tgctccatgt tccggtgagg tgatggaggc gcttcaggcc tcgggaatcg
     4261 actacaccat ctttttctac aacccgaaca ttcatcctca gaaagagtat ttaattcgta
     4321 aggatgagaa tattcgcttt gctgaacaac acggcgtgcc gtttattgat gctgattacg
     4381 acacagacaa ctggtttgaa cgagccaaag gaatggaatg ggagcccgaa cgagggatcc
     4441 gttgcaccat gtgttttgac atgcgttttg agcggacagc gctgtacgcc gctgaaaatg
     4501 gtttcagtgt gatcagcagt tcactgggca tttcacgctg gaaaaatatg cagcaggtta
     4561 acgactgtgg gcggcgagcc gtcgcgcatt atccgggcat ggtgtactgg gattataact
     4621 ggcgcaagca gggcggctcg tcccgtatga ttgaaatcag caagcgcgaa aaattctatc
     4681 agcaggaata ttgtggctgt gtgtattctc tgcgcgatac caatctacac cgcaaatctc
     4741 agggacgccc tcttatcaaa attggtcaac tccactacgg taaagaagag aaggagtgat
     4801 tttatggggc acctttctga ttgatttcat attggcgagg taagtagaat gactgcgggt
     4861 gcttgaggct atctgcttca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
     4921 aacattacgc gtccggcaag acgcttaaca ttaatctgag gccaatttca tgctttgcac
     4981 atgtaggtta gcctcttaca tgccgaaagg caaggagaag caggctatga agcagcaaaa
     5041 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     5101 gaggaaagac ctctgtgagg tacgaatccg aaccggccag acggaggtcg ctgtcttcgt
     5161 agactacgaa tccaggaagt aagagtgacc gggcggggag ctgatcccat ccccgcccac
     5221 ctctgatgtg tcaggcatcc tcaacgcacc cgcacttaac ccgcccatcg ctgtgatctc
     5281 tcagcgtttc ggcgggtttt ttgttgttta tttccggtga atttgattcg cgcaccttcg
     5341 cagatagaat cgactcactt aagtagcgcg cagggagaag agggatggac cccgaacagg
     5401 ggagagctat ttatctggaa ggattctgaa gatgaaaatc gaagaattgc gtgaaatttt
     5461 tagtgaaaat ggcctctatg ctgtgcgcgt tgagaatgga gcgattgtca gccattgccg
     5521 cattagatgt ttgcaatctc aacaaaggaa gagcggtgct gtgttatttt atttttgtaa
     5581 tggacttctg acggacggtt ttattttgcg tgaggacgaa tttgtcacat cattacgggt
     5641 tttgaaagag attggtttta aggctggttt tctgcttttg ctgaagaata aactcatcta
     5701 caatcttgag caggattgaa ctcctgctgt gtaacaccgt gccaccggag aaagccgatg
     5761 gcacatatac aactggtcaa acaaacctct tccggattac ttctcccggc gacgccggag
     5821 agttgcgatt ttctgcatca aatcaaaata ggtgagtgga tacacgcaga ctttaagcgt
     5881 gtgtgtaact acgcattcca caagcgtttt ttcaaactcc tgcaactggg tttcgattac
     5941 tggactccgg tcggtggggc gatcacgcct cgcgaacgaa aactggtgtc cggtttcgtt
     6001 gattacctgt gtgaatcagt aggccgggaa catacgccag ctctgagcga agccgcagag
     6061 caatatctga atacagttgc gacacgcaga accagtgata cggcattgct aaagtcgttt
     6121 gaggctttcc gcgagtgggt agccattcag gccggatttt acaccgagca tttttatccg
     6181 gacggtagcc gtgggtgtcg ggcgaaatct atcgc
//
LOCUS       DAZOZY010000218         6135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZOZY010000218 DAZOZY010000000
VERSION     DAZOZY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.220"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1255)
                     /locus_tag="ACHURQ_003702"
     CDS             complement(<1..1255)
                     /locus_tag="ACHURQ_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFR0825377.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKILSKDVLPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="ACHURQ_003703"
     CDS             complement(1412..1621)
                     /locus_tag="ACHURQ_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825378.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="ACHURQ_003704"
     CDS             complement(1644..2354)
                     /locus_tag="ACHURQ_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0825379.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..3193)
                     /locus_tag="ACHURQ_003705"
     CDS             complement(2870..3193)
                     /locus_tag="ACHURQ_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825380.1"
                     /translation="MNEYELQIFIDSDTAMMVQAFADSGVSIDLDKLLKLMAENSEEI
                     EDFIQEIETGEPRFMFPVTDSNMKRLIIEETNKYSVSPEKYLKAAIAILYSDNILVTD
                     SKVVH"
     gene            complement(3372..3572)
                     /locus_tag="ACHURQ_003706"
     CDS             complement(3372..3572)
                     /locus_tag="ACHURQ_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825381.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
     gene            complement(3572..3844)
                     /locus_tag="ACHURQ_003707"
     CDS             complement(3572..3844)
                     /locus_tag="ACHURQ_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0825382.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            complement(3847..4125)
                     /locus_tag="ACHURQ_003708"
     CDS             complement(3847..4125)
                     /locus_tag="ACHURQ_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825383.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            complement(4126..4683)
                     /locus_tag="ACHURQ_003709"
     CDS             complement(4126..4683)
                     /locus_tag="ACHURQ_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825384.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
BASE COUNT         1747 a         1275 c         1069 g         2044 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaagaac atctttagaa agaatcttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccatccat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcttttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtca attgatacac
     3121 cggaatcagc gaaagcctgc accatcatag cagtgtcgct atcaatgaaa atctgtaatt
     3181 catattcgtt catttttaaa atctcctttt taccagacct cctcaatcgc tctgtaacga
     3241 gctaaacagt ctttttcagg ggtagcctgt acagtggtat tacccaccct aaaataagat
     3301 agttttttgc gttagaatgc gattgagagc gtctgataag ctgtttttct aatttctctt
     3361 aaagtctcgg actaccaata acctaattcc gctttatttc gttcaaacca ttccgctgta
     3421 actgctgtct ttagtgttgc gtttgggctg gtggcatgaa tgaagatatc ttttggattg
     3481 caagcatcac cgaaaccacc ttctttgatg agcaggtctg atacatgctt aatcatttta
     3541 tctgtcaaat attcgaattt aatacataac attaaatctc caggagtaaa ttgtttgggt
     3601 ggcaatttct tttattacca tccctgaaga taatcttttt agttgcctga acttcatgat
     3661 gcaaaaacca gatgacatac ttcagataat aatcacgagt gccataacga atcattggat
     3721 taccgtaagc atcatctctt gtcttaacct ctccaccatc aatccattca agcattcctg
     3781 tctcataatt aatcatgaag taagttgcaa agaaccgttt aatatcatct cgccattgct
     3841 tcataatcag acctcctgag acagtttgtg aattttgata cttagtaacc ttgctaaatc
     3901 cagtccaaaa gcatttaagg atttcagact atcaaggtca tcttgcttaa tcctgatatg
     3961 gtgtcttgca gcctctaagc gactattaag caaatcctgt tcgttaatat cacggttaat
     4021 tgcaagcttc atcactgctg agtgtggcag acctgtaatg tttttgattc ggttcagttc
     4081 atcaaaggct gtctttgtaa ctctggttaa tgtgtgttta ttcatttaaa aatcctctgt
     4141 aactaaatag gggtcaaact cgaaatctgg ttgctcgttt tccattggct caatattccc
     4201 aacggcaata tgatgttcaa caatgccaat taatttcgcg cacatcacag caagcaaagc
     4261 gcaatattta tcgcttggta attcgtaact aagtctggtt gcctgtcgct gaagatgatt
     4321 tgtcacggtg gatgcgttaa tttcgtcaag attctttagc agataaaata gttttttgtt
     4381 gtatttcggg tggccttcac ttctacagaa cccaataaaa acatcatcaa gcttgcttgg
     4441 taactgctcg taactgtcaa agttagccag ataattttta atagtattct ttgttgtttt
     4501 gcgtactggc ataaatctgt ctcgtggcgt aatgctacga atgaccgctt caatgttctt
     4561 acgttcggat ttgctaaagg ttgggacttc gtagctggtg gggttgtttg tgtaagcgta
     4621 ggagttgtgc ttgttgcgtt ctcttgatgc ctgaagcatc gctctaaatt ctgaatactg
     4681 cattcatgtt ctgactctca taagatgtta ttattctgtt aacctatata tacaatatta
     4741 gcattttcaa agactaatgt caagtatttt taacattttc gtaacatctg tggtataatg
     4801 ggtttatggg gagagtaaaa tatgaggcgt ggctatgtat ttggtcgcat attttgcatt
     4861 gcacacagac ctcttcaatc gccctgtaac gagttaaacg acctcttcag gtcaactcat
     4921 aaggtcaggt actttttgtg tcagaatgcg attgagagcg tcttacaagg aaaactaatg
     4981 gtaaatctcc ctcctgagct ggggtcacct gatgtatgga ctggtggagg tattgggctg
     5041 attgcaggcc tgctacttcg ctacgtcctc gtcagagtgc ttgttgtctt taaactgttc
     5101 ggtcgtacat ggacgcttga ccttaaggat aacgaccaca cagacccccg ttaaactcct
     5161 tcaaaacccc tgcacgagat gacagataga agtctatggt acaggggtgt ctttagcatt
     5221 ttgtgctatc tattaaaaaa tagcttgaca gaaccggaaa gctgtatcta tcaagcattc
     5281 acctctctga cagtaatttc taaaagttct tatttttcaa taagttgtaa ttagccatat
     5341 atagagaact gacaagtctc ttcacagaca actctgcaac cttaacagta acttctaaaa
     5401 atgcttattt ttcaacgagt tgtaatcacc caatatataa ggctgtccag taccttcaca
     5461 gataacttca agccctctta accgccccac taatcgggag aagagcgata accccagaga
     5521 gggaagctcc tttcttaacc gacctcttaa acgaccttta cagaccttgt tcaaggtaat
     5581 cacaatagtt tttcttccct cggtttcctg tgttcctcgg ctggctattg agatttgaaa
     5641 gacctctgta agcgctctct tagcgagaga aaaatcattc atagtgaccc caagcgaagc
     5701 gcccctctaa aaaaagattt tttagctatt ttgtttaaaa gcatatcaaa cgatacattt
     5761 tattgattta ggtcaaagac aaaagcattg acaaaatggt aagattgttc acatgacctt
     5821 acttatttaa gagactttga aacagcctgt ttaacaggtc actacctctg gtcttatcgt
     5881 tcccttcttt ctaggtggtt atctgttaag agtttttcag tttttattct taagataatt
     5941 gttaagatga tactctccct tcgggttata tcgtatctct tctcccgtta aatatccctt
     6001 cacagtcaga ctaaataaca cctgtgtagt tcttggtaga atctttcaag gtaacttagc
     6061 tctgagttta tgaagttgac ctatgagata gactctaccg aatctgttag tcaattgttg
     6121 cttaggtgct gctaa
//
LOCUS       DAZOZY010000219         6127 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZOZY010000219 DAZOZY010000000
VERSION     DAZOZY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.221"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(225..1895)
                     /gene="betA"
                     /locus_tag="ACHURQ_003710"
     CDS             complement(225..1895)
                     /gene="betA"
                     /locus_tag="ACHURQ_003710"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFR0825385.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1909..3381)
                     /gene="betB"
                     /locus_tag="ACHURQ_003711"
     CDS             complement(1909..3381)
                     /gene="betB"
                     /locus_tag="ACHURQ_003711"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFR0825386.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3395..3982)
                     /gene="betI"
                     /locus_tag="ACHURQ_003712"
     CDS             complement(3395..3982)
                     /gene="betI"
                     /locus_tag="ACHURQ_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFR0825387.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4121..6073
                     /gene="betT"
                     /locus_tag="ACHURQ_003713"
                     /pseudo
     CDS             4121..6073
                     /gene="betT"
                     /locus_tag="ACHURQ_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
BASE COUNT         1243 a         1702 c         1705 g         1477 t
ORIGIN      
        1 ttgttcactc ataatatatc cagaaaataa aatctgccgc agataaggtt gtattaatag
       61 tctgtatcag gaatgttcgg ggtaaatatc agcaaaaaac cagcatcatg aatactggat
      121 atgaagcatg agagttacct cagtgtttat ataaggattc ggtccccctc tctggaacgg
      181 taactctcaa tctgatcggt tcctgcgtta gttcacatca cgactcattt tttcgctctc
      241 accggcatcc catttgccac aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca
      301 cgaatcatat ccgctatttt ctcgccaatc ataattgtcg tggcgttcaa attcccggtg
      361 ataatctgcg gcataattga cgcatccacc acgcgcaggc cttccagccc atgcacgcgg
      421 ccttcgccgt caaccacgga catctcgtcg taacccattt tgcaggtacc gcacggatgg
      481 aaggcggttt cggcgtgatt acgcacgaac tcatcgagct gctcatccgt ctgacattcc
      541 gtgccggggc tgatttcgcg gccacgatac tgatccagcg tcggttgatg catgatctcc
      601 cgggtgatgc gaattgcgtc gcggaactcc tgccagtcct gctcgtgtga catgtagtta
      661 aacagaattg ccggatgctg gtgcgggtcg cgggatttaa tccgcacatg cccacggctt
      721 ggcgagcgca ttgagccgac gtggcactgg aaaccgtgct ccttcaccgc attcgagccg
      781 ttatagttaa tcgctactgg caggaaatgg tactgaatat tcggccacgc aaattcctct
      841 cggctgcgaa taaatccgcc tgcttcaaag tggttgctgg caccaacgcc agtgccgcca
      901 aacagccact ccgcaccgat tttcggctgg ttccaccact gcagggcagg gtagagggaa
      961 accggttctt tgcactcata ttgcagatac atctccagat gatcctgaag attttcgccg
     1021 acgccgggta attcatgcac cagcggaata tcaaactccg ccagcagttc agcgttgccg
     1081 acgccggagc gttgcaggat ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct
     1141 ttgttggccg ttgcgcgggt tgggatggtg ctgtcgcctt ccagccattc gacgcccacc
     1201 gcgcgtttgc cgtcaaaaat gatgtgatcg gtcatagcgt gagtacgaat ggtcaggtta
     1261 gggcgcgatt tggcctgatc gagataaccg cgcgcggtac tggcgcgacg gccctgcggc
     1321 gtgacggtgc gatccatcgg accaaaacct tcctgctgat aaccgttgag atcgtccgtg
     1381 cgcgggtagc ccgcctgcac gcccgcttca atcatcgctt caaacagcgg attgacgccg
     1441 ggtttggagg tagtgacgct caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca
     1501 tcgcgagtct cggccttgcg gtagtagggc aggcagtcga gataactcca gttctccaga
     1561 ccgggttctt gcgcccagtt atcgagatcc agcgcattgc cacggatgta gcacatgccg
     1621 ttgatcagcg acgatccacc caggccttta ccgcgtccgc actccatgcg gcggttattc
     1681 ataaacggtt caggctctgt ttcataggcc cagttgtagc gtttgccctg tagtgggaac
     1741 gccagcgctg cgggcatctg ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca
     1801 agcagcagca cggaggtatt cggatcttca gtcagacggg tagcgagaac gttgccggct
     1861 gagccggcac caataatgat gtagtcaaat tgcaaataaa cctcctggtt agaatatgga
     1921 ctggaattta gccatctcaa cctggatgga cttcacctgg gtgtaactct ggagcgtcat
     1981 cacgccgttc tcgcgaccaa tgccggagtg tttgtagccg ccaacgggca tctctgccgg
     2041 ggattcgccc caggtgttga tccagcaaat acccgcttcc agctgatgaa tgacgcgatg
     2101 cgcgcggttc aggtccgccg tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc
     2161 gcggcgaatc acttcgtcct cagattcata agtgagaagc gacatcaccg gaccgaagat
     2221 ctcttcgcgc acgatggtca tttcgtcgcg gcaatcggtg aacacggtcg gtgcaaccca
     2281 tgcgccgtta tcgaagccat cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc
     2341 ttcctctttg cctttggcga tatagcgcag cacgttatcg cgatgcggga agctgaccag
     2401 cgggccgaag ttagtttgcg gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc
     2461 cagaattttc tgctcaaatg cggctttgca tttcgccgga acgaagacgc gggtgccatt
     2521 ggtacacacc tgaccggagc tgaagaagtt tgccatcatg gcgatatcgg cggcgagatc
     2581 gagatccgca tcatcgaaaa cgatcagcgg tgatttaccg cccagttcca tggtcacttc
     2641 tttcagggaa gaggccgccg agttagccat cacttttttg ccgctggcga caccgccggt
     2701 aaatgacact ttggcaatgc ccggatgctc ggtcagatat tgcccggtct ccgcgcccac
     2761 ccccggcaac acgttaaata cgccgtccgg caggcccgct tcgctgtaaa tttcagccag
     2821 ctttaacgcg gtaagcgggg tgacttcgct cggtttgaaa atcattgcgt tgcctgccgc
     2881 cagcgccggg gcggatttcc acagggcaat ctgaatcggg tagttccatg cgccaatccc
     2941 cgccaccaca cccagcggtt cgcggcgggt ataaacaaat gacgtctcac gcaacgggat
     3001 ctggctgcct tccagcgccg ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt
     3061 aacgatatcg acggttgagg tttccgaata tgcttttccg gtatcgaggg tttccagttt
     3121 tgcgagttcg tcattgcgtt cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg
     3181 ctccatagcg gtcatcgccg cccagatttt ttgcccctgt tgggcactct tcacggcgcg
     3241 atcgacatcc tcacgcccgg cggcctgcac ggtcgccagc acgttaccgt tggccgggtt
     3301 aatggtctcg aaggtgcgac cgctggtggc ggaggtataa ccaccatgta tataaagctg
     3361 ctgttctgcc attcgggaca tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa
     3421 taaagtgacg ggtcagcgag tgtgcgagag gtttatccag cggtttgccg cttagtgccg
     3481 cgcgcagcca taatccgtca atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt
     3541 cgcgcggcaa ttcgcggcga aactcgctca ccagattcga cagcaagcgg cggctgctga
     3601 cctgctgtaa acgatagagc atcggctgat gcatactgct ggcccagaac gccagccagg
     3661 ctttcatcgc cgcactgctc acctgagttt catcgaagtt tccggcaaca atcgcctgta
     3721 atcgctgctc tgcactgccc tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca
     3781 gctgactggt gatatcgcgc atggttgctt ccagcagacc atttttgtcc ctgaaatagt
     3841 ggctgatgat ccccgtagaa acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt
     3901 gcatgcccac ttcatttatt gcttccagtg tggcgtcgat cagttgtctg cgccggatcg
     3961 actgcatccc caatttgggc attttcgcca ctccattcat cagcgttgtt tatctattaa
     4021 agcggttatt gattggacgt tcaatataaa atgtgtcgta attgttacga atttgatttt
     4081 aaatggtaac aataacagtg gggatactgg atgacagacc ttttccctga caacgatcct
     4141 gtttcgcgac ttctcggccc tgtggattgg ccgcacgctg gactgggttt ctaaaacctt
     4201 cggttggtac tatctactgg cggcaacgct ctatattgtc tttgtggtct gtatcgcttg
     4261 ttcgcgtttt ggttcggtga agctcgggcc agaacaatcc aaaccggaat tcagcctgct
     4321 gagttgggcg gcgatgctgt ttgctggcgg gatcggtatc gacctgatgt tcttctccgt
     4381 agccgaaccg gtaacgcagt atatgcagcc gccggaaggc gcgggacaga cgattgaggc
     4441 cgcgcgtcag gcgatggtct ggacgctgtt tcactacggc ttaaccggct ggtcgatgta
     4501 tgcgctgatg ggcatggcgc tcggatactt tagctatcgt tataatttgc cgctcaccat
     4561 ccgctcggcg ctgtacccga tcttcggtaa acggattaac gggccgatag gtcactcagt
     4621 ggatattgca gcggtgatcg gcactatctt cggtattgcc actacgctcg gtatcggtgt
     4681 ggtgcagctt aactatggct tgagcgtact gtttgatatt cccgattcga tggcggcaaa
     4741 agcggcactg atcgccttgt cggtgataat cgccacgatc tctgtcacct ccggtgtcga
     4801 taagggcatt cgtgtgttat cggaacttaa cgttgcgctg gcgctgggat tgatcctgtt
     4861 cgtattgttt atgggcgaca cctcgttcct gcttaatgcg ctggtgctga acgttggcga
     4921 ctatgtgaat cgctttatgg gcatgacgct caacagtttt gccttcgacc gtccggttga
     4981 gtggatgaat aactggacgc tcttcttctg ggcatggtgg gtggcatggt cgccgtttgt
     5041 cggcttgttc ctggcgcgta tctcgcgtgg gcgtaccatt cgccagttcg tgctgggcac
     5101 gttgattatt ccgtttacct tcacgctgtt atggctctcg gtattcggca atagcgcgct
     5161 gtatgaaatc atccacggcg gcgcggcatt tgccgaggaa gcgatggtcc atccggagcg
     5221 cggcttctac agcctgctgg cgcagtatcc ggcgtttacc tttagcgcct ccgtcgccac
     5281 cattactggc ctgctgtttt atgtgacctc ggcggactcc ggtgcgctgg tgctggggaa
     5341 tttcacctcg cagcttaaag atatcaacag cgacgccccc ggctggctgc gcgtcttctg
     5401 gtcggtggcg attggtctgc tgacgctcgg catgctgatg accaacggga tatccgcgct
     5461 gcaaaacacc acggtaatca tggggctgcc gttcagcttt gtgattttct tcgtgatggc
     5521 ggggttgtat aaatctctga aggtagaaga ttaccgccgt gaaagtgtca accgcgatac
     5581 cgcgccgcga ccgctggggc ttcaggatcg cctgagctgg aaaaaacgtc tatcgcgcct
     5641 gatgaattat ccgggcacgc gttacactaa acagatgatg gagacggtct gttacccggc
     5701 aatggaagaa gtggcgcagg agctgcggtt gcgcggcgcg tacgtggagc taaaaagcct
     5761 gccgccggaa gagggacaac agttggggca tctggatttg ttggtgcata tgggcgaaga
     5821 gcaaaacttt gtctatcaga tttggccgca gcaatattcg gtgccgggct ttacctaccg
     5881 cgcacgtatc ggtaaatcga cctactaccg actggaaacc ttcctgttag aaggcagcca
     5941 gggcaacgac ctgatggact acagcaaaga gcaggtgatc accgatattc ttgaccagta
     6001 cgagcggcac cttaacttta ttcatctcca tcgtgaagcg ccgggccata gcgtgatgtt
     6061 cccggacgcg tgattgtttt tgcataaaac cgcgccatgt ctgcatatgg cgcgggaatc
     6121 attgact
//
LOCUS       DAZOZY010000220         6124 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZOZY010000220 DAZOZY010000000
VERSION     DAZOZY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.222"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            71..847
                     /gene="exuR"
                     /locus_tag="ACHURQ_003714"
     CDS             71..847
                     /gene="exuR"
                     /locus_tag="ACHURQ_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR0825388.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1087..1170
                     /gene="yqiM"
                     /locus_tag="ACHURQ_003715"
     CDS             1087..1170
                     /gene="yqiM"
                     /locus_tag="ACHURQ_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR0825389.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1192..1854
                     /gene="yqjA"
                     /locus_tag="ACHURQ_003716"
     CDS             1192..1854
                     /gene="yqjA"
                     /locus_tag="ACHURQ_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR0825390.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1858..2241
                     /gene="mzrA"
                     /locus_tag="ACHURQ_003717"
     CDS             1858..2241
                     /gene="mzrA"
                     /locus_tag="ACHURQ_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR0825391.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2388..2756
                     /gene="yqjC"
                     /locus_tag="ACHURQ_003718"
     CDS             2388..2756
                     /gene="yqjC"
                     /locus_tag="ACHURQ_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR0825392.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2794..3099
                     /locus_tag="ACHURQ_003719"
     CDS             2794..3099
                     /locus_tag="ACHURQ_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR0825393.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3102..3506
                     /locus_tag="ACHURQ_003720"
     CDS             3102..3506
                     /locus_tag="ACHURQ_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR0825394.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3496..3795
                     /locus_tag="ACHURQ_003721"
     CDS             3496..3795
                     /locus_tag="ACHURQ_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR0825395.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3981..4373
                     /locus_tag="ACHURQ_003722"
     CDS             3981..4373
                     /locus_tag="ACHURQ_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR0825396.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4443..5428
                     /gene="yqjG"
                     /locus_tag="ACHURQ_003723"
                     /pseudo
     CDS             4443..5428
                     /gene="yqjG"
                     /locus_tag="ACHURQ_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5721..6086
                     /locus_tag="ACHURQ_003724"
     CDS             5721..6086
                     /locus_tag="ACHURQ_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0825397.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1533 a         1512 c         1544 g         1535 t
ORIGIN      
        1 atgaccaatg gcatacccat gaataaagtg gtatgataac ttcgtccgca ttccactccg
       61 ggagacgcat atggaaatca ctgaaccacg ccgtttgtat caacaacttg ccgctgacct
      121 gaaagagcgc attgaacagg gcgtctatct ggtgggtgat aaactgcctg ccgaacgctt
      181 tattgccgat gaaaagaacg tcagccgaac ggtagttcgt gaagccatca tcatgctgga
      241 agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt catgtggttt ccaaccagcc
      301 gcgccatcag caggcggctg acaataatat ggaattcgcc aattacggtc cgtttgagtt
      361 gcttcaggct cgccagctca tcgaaagtaa tattgccgag ttcgcggcaa ctcaggtaac
      421 gaaacaagac atcatgaaac tgatggccat tcaggaacag gcgcgcggcg aacaatgctt
      481 ccgtgattcc gagtgggatt tacagttcca cattcaggta gccctggcga cgcagaactc
      541 cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt agccataacc cgtactggaa
      601 aaaactacac gaacacattg attcccgtac cgttgataac tggtgtgatg accacgatca
      661 aattctcaag gcgttaattc gcaaagatcc ccatgctgct aagctggcaa tgtggcagca
      721 tctggaaaac accaaaatca tgttatttaa cgaaaccagc gacgatttcg agttcaatgc
      781 cgaccgctat ctattcgccg aaaacccggt agtgcatctc gatacggcca ccagtggcag
      841 taaatgaaac tcctgtccga caggcgctgc gacgcgcctg ttcgcttcgt cggtaagcga
      901 aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc ctcccctgca ttcagcaaaa
      961 tcagactcca cggacatgta attttgataa cgaacaacgt tgacctttgt tacaattaga
     1021 ttcaatttga atttatgttt ttgaatgctt tcttatctca cgatttaaca gggaatagtt
     1081 caggctgtgt tgatgtatca aacccgcaga acataccaaa acagcaataa cattgcggta
     1141 gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc aggaataatg aatggaactt
     1201 ttgacccaat tgctgcaagc cctgtgggcg caggattttg aaaccctggc caatccatcg
     1261 atgattggca tgttgtattt tgtcttgttt gtaattttgt tccttgaaaa cggcttgctt
     1321 ccagcggcct ttttaccggg cgacagttta ctggtattgg tcggcgtgtt gattgcgaaa
     1381 ggcgcgatgg gttacccgca aacgattctg ctgctgaccg ttgccgccag cctcggctgc
     1441 tgggtcagct atattcaggg gcgatggctg ggcaataccc gcaccgtaca aaactggcta
     1501 tctcatttac ccgcgcatta tcatcaacgc gcacaccatc tttttcataa acacggttta
     1561 tcggcgctgt taattggtcg ctttattgcg tttgtcagaa cactgctgcc gacgattgcc
     1621 gggttatcag ggctgaataa cgcgcgcttt cagtttttca actggatgag cggtctgctg
     1681 tgggtattga tcctgacaac tctgggttac atgctcggca aaacgccgat atttttaaag
     1741 tacgaggacc agctgatgtc atgcctgatg ctgctcccgg tggtgctgct ggtgtttggc
     1801 ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg gaaatcgggg gtaagggatg
     1861 caaatacctc gcatgtcgct tcgccagcta gcctggtccg gcactgtttt acttctggtc
     1921 ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag agtctacgct ggcgattcgt
     1981 gccgttcatc aaggcacaac gatgccagac ggtttttcga tctggcatca ccttgacgct
     2041 catggcattc ctttcaaaag tatcaccccc aaaaacgaca ctctgttaat tacatttgac
     2101 tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca gaacattgcc ccatggctac
     2161 atcattgcgc agcaggacaa taacagtcag gctatgcagt ggctgacccg gttacgggat
     2221 aactctcatc gcttcggata acttcctgtt atccgaaaca tatcactcac tttggtgatt
     2281 tcaccgtaac tgtctatgat taatgaggcg gtggttaaca ctaccgcttc gttgtctgga
     2341 caatcgttcc tttgtaatag gtccgaatca caatggaagg ttcaagaatg aaataccgca
     2401 tcgctttagc tgtttctctc tttgctctta gtgccggtag ttatgccact accctgtgtc
     2461 aggaaaagga gcaaaatatc cttaaggaga tcagctatgc cgaaaaacac caaaaccaga
     2521 atcgtattga cggtctgaat aaagccctga gtgaagtccg ggccaactgt tcagatagcc
     2581 agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa agatgaggtg gcggaacgcc
     2641 agcaagattt agccgaggcg aagcaaaaag gcgatgccga taagattgcc aaacgcgaac
     2701 ggaaactggc agaagcgcag gaagagctga aaaagctgga agcgcgcgac tactaactca
     2761 caatagtcac tacttactca cctggagaaa actatgtcga aagaacacac tacggaacat
     2821 ctgcgtgctg agttgaaatc cctttccgat acgctggaag aggtgcttag ctcatctggc
     2881 gagaagtcga aagaagagtt gagtaagatt cgtagcaaag cggagcaggc actgaaacag
     2941 agccgttatc gcctgggtga aaccggtgat gccatcgcca aacaaacccg tgtcgcggcg
     3001 gcgcgtgctg atgagtatgt gcgtgaaaat ccgtggaccg gcgtgggaat tggcgctgca
     3061 atcggtgtag tgctcggcgt tctgctgtcg cgtcgttaat tatggcggac actcatcacg
     3121 cgcaagggcc cggtaaaagc gttctgggca tcgggcagcg aattgtttcc atcatggttg
     3181 aaatggtgga aacacgtctg cggctggcgg tggtggagct ggaagaggaa aaagctaatc
     3241 tctttcaact tttactgatg ctgggcctga cgatgctttt cgctgcattt ggtcttatga
     3301 gcctgatggt gctaattatt tgggcggttg atccgcaata tcgcctgaat gcgatgattg
     3361 ccaccaccgt ggtgttgctg ctactggcac tgattggcgg tatctggacg ttacgtaaat
     3421 cgcgtaaatc caccttgctg cgccatacac gccatgagct ggcaaacgat cggcaactgc
     3481 tcgaggagga gtcccgtgag cagtaaagtc gaacgtgaac gacgtaaggc gcaactgctt
     3541 agccagatcc agcaacaacg gctggatctt tccgccagtc gtcgtgaatg gctggaggca
     3601 acaggcgctt acgatcgtcg ctggaatatg ctgctaagtc tgcgctcctg ggcgctggtt
     3661 ggcagtagcg tgatggcgat ctggacgatt cgccatccta atatgctggt ccgctgggcc
     3721 agacgcggtt ttggcgtatg gagcgcctgg cgtctggtta aaacgaccct caagcagcaa
     3781 cagcttcgcg gttaacttcc ctctggccgg agcaattccg gccttatccc tcaaattttt
     3841 tgaagatttt tgacagtttt ccttgctaac aatcatcatt caccacgttt atgattctct
     3901 ccatcgacag caacgacgct aataccgcgc ccttgcacaa aaaaacaatc agcagcctga
     3961 gtggcgcagt ggagagtatg atgaaaaaat tagaagatgt tggtgtactg gtagcgcgca
     4021 ttttaatgcc gattctgttt attaccgcag gctggggaaa aatcaccgct tactctggta
     4081 cccaacaata tatggaagca atgggtgtcc cgggttttat gctgccgctg gtgattctgc
     4141 ttgagttcgg tggtggtctg gcaatcctgt tcggtttcct gactcgcacc acagccctgt
     4201 ttactgcggg ctttacgctg ctgacggcat ttttatttca cagcaacttt gctgaaggcg
     4261 tcaactcgct gatgttcatg aaaaacctga caatttctgg cggattcctg ctgctggcaa
     4321 ttaccggtcc gggcgcgtat agcatcgacc gcctgctgaa taaaaagtgg taagcacgcg
     4381 ctatacttaa cgataaaaaa cgaggaggaa gctcctcgtt tttgctattg gaggagagaa
     4441 aaatgggtca actgattgac ggcgtctggc atgacacctg gtacgatacc aaatctaccg
     4501 gcggtaaatt tcaacgttca gcttccgcat ttcgtaactg gctcactgcc gatggcgcac
     4561 ctggccccac tggcacaggc ggttttatcg cagagaaaga tcgttatcat ctctatattt
     4621 cactcgcctg cccgtgggcg caccgcacgc tgatcatgcg caaactcaaa ggactggaac
     4681 cgtttatttc cgtttccgta gtgaacccgc tgatgctgga aaacggctgg acctttgatg
     4741 acagttttcc gggagcaacc ggagatacgc tctatcaaca tgaatttctg tatcagcttt
     4801 atctccacgc cgatccacac tacagcggac gagttactgt tcccgtactg tgggacaaaa
     4861 agaaccacac catcgtcagc aacgaatcaa cagaaatcat ccgcatgttc aataccgcgt
     4921 ttgatgcgct gggcgcgaaa gcgggtgatt actacccacc agccctgcaa acgaaaattg
     4981 acgaacttaa cggctggatt tatgacaccg ttaacaacgg cgtgtataaa gctggttttg
     5041 ccaccagcca gcaagcctac gacgaggcgg tggcgaaagt gtttgaatcg ctggcgcgac
     5101 tggagcagat tttaggccag catcgttacc tgaccggcaa ccagctaacc gaagccgata
     5161 ttcgcctgtg gactacgctg gtgcgttttg atccagtgta tgtgacccac ttcaagtgtg
     5221 ataagcaccg catcagcgat tacctgaatc tgtatggctt cctgcgcgat atctaccaga
     5281 tgccggggat cgccgaaact gtcaatttcg atcacatccg taatcattac ttccgcagcc
     5341 ataagaccat caaccctacg gggattattt caattggtcc gtggcaggat ctcgatgaac
     5401 cgcaggacga gatgttcgct tcggttaaaa aaaggcacct tacgaagtgc ctctgataat
     5461 tagctaagtg catgttgttc atatcggatg cggcgtgaac gccttatcaa acctacaaaa
     5521 catttatatt caatatgttg caatgaccgc gcaggccaga taagcgtagc gcatctggcg
     5581 attttgcgct tgtcatcagt cttagcgcgc cttcacagca ctttattaat tccttgaacg
     5641 aatatttact gccatttcat tcacgtctat tcttaatttg ctgctttaaa aacaagtgat
     5701 tgagcaaaat tgaggcaaaa atggactggt atctgaaagt aataaaaaat tatgtcggtt
     5761 tccggggtcg tgcgcggcgc aaagagtact ggatgtttat tctggtcaac atcatcttta
     5821 cgttcgtgct ggggctgctg gataaaatgt taggctggca acgcgctggt ggcgaaggca
     5881 tcctgacgac aatctacggt attctggtgt ttttaccgtg gtgggcagtt cagttccgcc
     5941 gcctgcacga caccgaccgc tcggcgtggt gggcactgct attcttaatc ccgtttatcg
     6001 gctggcttat tatcatcgtc tttaactgcc aggcgggtac gccaggcgaa aaccgctttg
     6061 gacctgatcc aaaactggaa ccttaaaaca aatctgctcg ccggatgtct gactcatccg
     6121 gctt
//
LOCUS       DAZOZY010000221         6121 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZOZY010000221 DAZOZY010000000
VERSION     DAZOZY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.223"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..358
                     /locus_tag="ACHURQ_003725"
     CDS             <1..358
                     /locus_tag="ACHURQ_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0825398.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="ACHURQ_003726"
     CDS             complement(398..1069)
                     /locus_tag="ACHURQ_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR0825399.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHURQ_003727"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="ACHURQ_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR0825400.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHURQ_003728"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="ACHURQ_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR0825401.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /gene="yedD"
                     /locus_tag="ACHURQ_003729"
     CDS             2798..3211
                     /gene="yedD"
                     /locus_tag="ACHURQ_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR0825402.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHURQ_003730"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="ACHURQ_003730"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR0825403.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHURQ_003731"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="ACHURQ_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR0825404.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHURQ_003732"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="ACHURQ_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR0825405.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHURQ_003733"
     CDS             complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="ACHURQ_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0825406.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1497 a         1609 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gcgataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 a
//
LOCUS       DAZOZY010000222         6056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZOZY010000222 DAZOZY010000000
VERSION     DAZOZY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.225"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHURQ_003734"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHURQ_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR0825407.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHURQ_003735"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHURQ_003735"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR0825408.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHURQ_003736"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHURQ_003736"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR0825409.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHURQ_003737"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHURQ_003737"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR0825410.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHURQ_003738"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHURQ_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR0825411.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1434 a         1530 c         1646 g         1446 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaa
//
LOCUS       DAZOZY010000223         6025 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZOZY010000223 DAZOZY010000000
VERSION     DAZOZY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.226"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(127..276)
                     /locus_tag="ACHURQ_003739"
     CDS             complement(127..276)
                     /locus_tag="ACHURQ_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825412.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            847..1005
                     /locus_tag="ACHURQ_003740"
     CDS             847..1005
                     /locus_tag="ACHURQ_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0825413.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
     gene            1465..2280
                     /gene="sul2"
                     /locus_tag="ACHURQ_003741"
     CDS             1465..2280
                     /gene="sul2"
                     /locus_tag="ACHURQ_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0825414.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2367..2669
                     /locus_tag="ACHURQ_003742"
     CDS             2367..2669
                     /locus_tag="ACHURQ_003742"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825415.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(2845..4338)
                     /locus_tag="ACHURQ_003743"
     CDS             complement(2845..4338)
                     /locus_tag="ACHURQ_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFR0825416.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(4382..4771)
                     /locus_tag="ACHURQ_003744"
     CDS             complement(4382..4771)
                     /locus_tag="ACHURQ_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825417.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4989..5429
                     /locus_tag="ACHURQ_003745"
     CDS             4989..5429
                     /locus_tag="ACHURQ_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR0825418.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1428 a         1663 c         1581 g         1353 t
ORIGIN      
        1 aaaaaaaagg aatatttcgt tttcacgagt aatacccggg tattaaaaat ccactgtaat
       61 tacatgaaaa taaagaaata aaaggtaata ccggagtatt accacgtacg cgggcaagcg
      121 aaaaagctat aacgcttccc ggccgacagg cgacccaaaa tccacagggg catgcaggtt
      181 ctcttcagta acctgcgcca ttaaatcaaa aaggtcatat tcgccgggaa gctgcgtatc
      241 agtcgaatcc agttgttcat catattcaga tggcatgggg tgatatccag tagctgagag
      301 gttgttagcg ggtttcgggg cgcagccctg aaccagtcat gtagcgctag cggagtgtat
      361 actggcttac tatgttgcca cgattgcgaa aatcagtgaa gtgcttcatg tggcaggaga
      421 aaaaaggcag caccggcgcg tcagcagaat atgcgataca ggagatattc cgcttcctcg
      481 ctcactgact cgctacgctc ggtcgttcgg ctgcggcgag cggtaccggc ttacggacgg
      541 ggacggaaat ttcctggaag ataccaggaa gacacttagc aaagaagaga taaggccacg
      601 gcgaagccgt ttttccatag gctccgcccc cctgacaagc atcgcaaaat ctgacgctca
      661 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
      721 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
      781 ccgcggtttc tcattccacg cctgacactc agttccgtga aggcagttcg ctccaagctg
      841 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttgtccgg taactatcaa
      901 cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccat tagtaacagg
      961 agtgcacgag aagatagatt aagaggtctt gaagtagagg cctgactgcg gctacactga
     1021 aagaacagtt ttggtaatgg cggtctcgtg aaccctgtta ccgggttaag cagttcccca
     1081 actgacttaa ccttcgaaca aaccgcctcc ccaggcggtt ttttcgttta cagagcaaga
     1141 gattacgatt cattgaaaag gatctcaaga agatcctttg tagattacta tcaattaatc
     1201 ttccgaacat gacaccagtc actgttgatt acggttaaac cgctccgtaa caggtttccc
     1261 tcgtattggt gacttgcagc accaaagaag caaaaaagat aaatatatct acttattgag
     1321 agatgatata tttatttcca aaatgctaca gatacatcat catgaaacgg atcaccgcaa
     1381 acaggttact cgcctgggga ttccctttcg acccgagcat ccgtatgaga ctcatgctcg
     1441 attattatta ttatagaagc ccccatgaat aaatcgctca tcattttcgg catcgtcaac
     1501 ataacctcgg acagtttctc cgatggaggc cggtatctgg cgccagacgc agccattgcg
     1561 caggcgcgta agctgatggc cgagggggca gatgtgatcg acctcggtcc ggcatccagc
     1621 aatcccgacg ccgcgcctgt ttcgtccgac acagaaatcg cgcgtatcgc gccggtgctg
     1681 gacgcgctca aggcagatgg cattcccgtc tcgctcgaca gttatcaacc cgcgacgcaa
     1741 gcctatgcct tgtcgcgtgg tgtggcctat ctcaatgata ttcgcggttt tccagacgct
     1801 gcgttctatc cgcaattggc gaaatcatct gccaaactcg tcgttatgca ttcggtgcaa
     1861 gacgggcagg cagatcggcg cgaggcaccc gctggcgaca tcatggatca cattgcggcg
     1921 ttctttgacg cgcgcatcgc ggcgctgacg ggtgccggta tcaaacgcaa ccgccttgtc
     1981 cttgatcccg gcatggggtt ttttctgggg gctgctcccg aaacctcgct ctcggtgctg
     2041 gcgcggttcg atgaattgcg gctgcgcttc gatttgccgg tgcttctgtc tgtttcgcgc
     2101 aaatcctttc tgcgcgcgct cacaggccgt ggtccggggg atgtcggggc cgcgacactc
     2161 gctgcagagc ttgccgccgc cgcaggtgga gctgacttca tccgcacaca cgagccgcgc
     2221 cccttgcgcg acgggctggc ggtattggcg gcgctgaaag aaaccgcaag aattcgttaa
     2281 ctgcacattc gggatatttc tctatattcg cggttcagca ggcatgtccc ctttgagggc
     2341 gacccgacga caggataatc gaccttatgg tgcgcaaata tttcggcaca gacggtattc
     2401 gtggcaaagc caacgaaggc gcgatgacgg cggaaaccgc cttgcgcgtc ggcatggcgg
     2461 ctggccgtgt ctttcgtcgc ggtgaccacc gccatcgtgt cgtgatcggc aaggatacgc
     2521 gcctgtcggg ctatatgctt gaacccgcgc tcacagccgg tttcacctcg atgggcatgg
     2581 acgtattcct ttttggcccg ctgccgacaa cgtataggaa gaataaacgc ccttttcacc
     2641 caagtccaac agctttggac cgcagttgac tctttcgaca cccctgcgat gcaacccaat
     2701 ccggctgacg gggagccagc aacgctgaaa atttaccctc ctctttccca ctagcggctc
     2761 cttttccgac aaccagcacg gcggatccct gccgcggcgc tgtgaacgca gcattttgat
     2821 tggtatcgtt ggccttcagg ctcgtcagtc aaacagaccc aggagcagct cagccggtgg
     2881 cgcccggctt tcgggtaacg ccctggtccc gctggtttcg gctttgtgct tcaggtgatc
     2941 aaggatctgc ttgatcacta tagggtcttc aatgcaggcg atgactttca tggcgccgcc
     3001 gcagccgctg caggtctcga tgtcgatatt gaaaacacgc ttgagccgtt gcgcccatgt
     3061 catcgacgct cgccgttgtg ctggtgttgc cggttcatca gccaccctga ccttgttgcc
     3121 cctgccccgt tttgccggcg tgaccaacgc ccggtgccga ctgttgggtg cgaacacccc
     3181 gtggaagcgg gttaggttga ctctgggctt cggtaccagg gcggccagcc ttgcaatgaa
     3241 atccaatggt tcgaaaatga cgtgcgtggt gccgtcccgg tacggcgtct tgagctggta
     3301 gcgcacgttg ccgcctcgtg ttaacgacag ccgcttctcg gataccgccg ggcggctgat
     3361 gtaccggcac agccgttcga gcttcttgcg ttcatcggcc ctggccgcca cgccggcgtg
     3421 caggctggac ccggctacct tgccaatccc gtcaccgaac ggatcaccac tggtcggcag
     3481 agtttgcaaa gtgaacacct ttcgccccgc ctgtgaaccg acagcgatac ggtaagtgat
     3541 cgagtgcccc agcaggggtg tcatcgggtc gtcatccacc gcatccgagg ccagatagct
     3601 gttttcgaca tcccgttcca gcaggccttg ccgttccaga tagcgaccca cccggtgggc
     3661 gatggtgtgc gtcagctggg tgagctctgg gctggtcggc gccttgaccc agcggaaacg
     3721 cgctgagccg tgggattgct cgacatacac accgtcgaga aacagcatgt ggaagtgaac
     3781 attcagattg agcgccgatc caaaacgctg gatcagggtg accgcgcccg tcttggccac
     3841 ttggtgggta tggcccgctt tcttgaccag gtgcgtggca atgacgcggt aaacgatgcc
     3901 cagcacccac cccatgatct cgggccggct ggcaaacagg aaacgcagct gaaacgggaa
     3961 gctcaacacc cactgacgca tgggttgttc aggcagtact tcatcaacca gcaaggcggc
     4021 actttcggcc atccgccgcg ccccacagct cgggcagaaa ccgcgacgct tacagctgaa
     4081 agcgaccagg tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag
     4141 ccgcccgcat tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc
     4201 ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag
     4261 ggtttgctcg ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct
     4321 ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg
     4381 cttagcggaa agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc
     4441 cagtgcccgt cactcccact cttacattgt tcgcagtatc taggtacatg ttattgcgtg
     4501 caccttcatg atgtataatg ttattaatga cagcagaaag atcgacagaa ttaatattct
     4561 tcgtacctgg gacagttatc gggtgtcggc cagtgatgag accaaaggat gcagtataag
     4621 atgaaccact actattatca tggccaccat taccaccacc gttaaaacta cctcccgaac
     4681 ttccagagcc gctatgacca tcattgtagg ggtcatttgg tcccatgtga ccaaaaccac
     4741 cgacagtacc gctatttaac tctgtactca taaaattatc ctctttgact tttaaaaaca
     4801 gcaaaaactg tatttataac aatgtgttta tatacagtat tcgaaatact acgcccgtca
     4861 aaaccacacc gtcaacaggt tttttgtgta tttttcacgc ctcacgggca gcctcagacc
     4921 ttttgtgtta aaacagtaat atgttctgct gttacaatac ggtacacaca ggagacactg
     4981 tcacttttat ggcatggcac acagttcaag agacactgaa attaacagga aagtcgcgct
     5041 cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta tcgcacagga aaaaatgatc
     5101 gccgggagtt cgagaccagt gaactgattc gggcctatgg cgagctgcgc acttctgaga
     5161 caccaaaaag gcacaaggag ggacactcca aatctgtcgt tagccaggcg gaaaatgctc
     5221 agctagacgt cattcagcag aaactggacg ctctgcagca gactgttacc ctaatgcttg
     5281 aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa tgaggagagg gaacaactac
     5341 aggccgaaat tagccgtctg gctgacgcgc tggcacagga aaaaaagcgg ggattctggt
     5401 cgcggctgtt tcgctccggt tcagagtaac agtgcaggtg actctgattt accataacat
     5461 ctgttatcag caccgcctga gaggccctca gggcgtttct gacgggaaac cggtaaaaca
     5521 ggcaacaaac cacccgaaaa cccgccagaa acgcgcaggc tctgttttta cggcatgcat
     5581 gagtatgcgc acaagtgcat agcgcggaaa ttcacaccat tcagccgggg taagtcctgt
     5641 ttccggcgtt cttcccatcc gaaaacggtg aacggggacg caatttattg cgtggccgtg
     5701 aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg ttcaggaagg gggtgatgta
     5761 caaatggcac agaggatgaa agtgacgtat gagctgaagg aatgatgtaa aagtgatgta
     5821 tgaactgaag gagcgatgta attctgtagt caggaagaat aacccgttca tgcaggcatg
     5881 aacgatacag tgaggaacgc tttttttgtc acgatctccc cttaacctgc gtaaatcacc
     5941 cgccatggtt gattattttt caacctcaaa atggtcatac acctaacgca gccgcgattt
     6001 tcatcgcaac tgcatgaatt aaaag
//
LOCUS       DAZOZY010000224         5988 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZOZY010000224 DAZOZY010000000
VERSION     DAZOZY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.227"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(147..509)
                     /locus_tag="ACHURQ_003746"
     CDS             complement(147..509)
                     /locus_tag="ACHURQ_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR0825419.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            1046..2959
                     /gene="mtlA"
                     /locus_tag="ACHURQ_003747"
     CDS             1046..2959
                     /gene="mtlA"
                     /locus_tag="ACHURQ_003747"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR0825420.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3189..4337
                     /gene="mtlD"
                     /locus_tag="ACHURQ_003748"
     CDS             3189..4337
                     /gene="mtlD"
                     /locus_tag="ACHURQ_003748"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR0825421.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4337..4924
                     /gene="mtlR"
                     /locus_tag="ACHURQ_003749"
     CDS             4337..4924
                     /gene="mtlR"
                     /locus_tag="ACHURQ_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR0825422.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4936..5145)
                     /gene="yibT"
                     /locus_tag="ACHURQ_003750"
     CDS             complement(4936..5145)
                     /gene="yibT"
                     /locus_tag="ACHURQ_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR0825423.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5430..5792
                     /locus_tag="ACHURQ_003751"
     CDS             5430..5792
                     /locus_tag="ACHURQ_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR0825424.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1526 a         1472 c         1550 g         1440 t
ORIGIN      
        1 cgagttaaaa agcggtagaa aaagttaagt tcccggttga tcgaatggat gcgaatcgct
       61 gatcgaactt cgatgccaat ccgtgatcga atagagcgga atacgcacca gcaccacgta
      121 agttaaaaca atcaatagat ccataattag atctccggga acgtggaata gtcagtcttt
      181 ttctcggcgg cgagttgatg ctctagccgg gaaattcggt cagaaagtgc ggcgatttcc
      241 gggtcagtcg ctttctcctg cgacgcaaca tgtgactgca tgccccaccc gcgctccggt
      301 tgatagagcg tcgcccagat ccacaggaac ggccagataa catgcagggt aaacaggctc
      361 acccagcccg ccgtatgaat agcgtcggca tggggatggt tgcgcttttt ggcaatcaga
      421 taagggatgt catgtatcgc gatgattcca taaaaaatca ccagaaagac gaagatcagc
      481 actcccaacg cgaaatagtt tagaaacata tctgcctcgg attcacgttt atcagtgttg
      541 tttttgggct ggcagccaga agggagtcag gctgatattt tgacaataat ccgggttcgc
      601 gattctcgcc ataacaccaa agaataattt ttagaggtga tgagttgctt agttacataa
      661 caattgtatg acgaaggcat aacatgctgt agatcacatc aggtgaacgc cgtaagaaaa
      721 tatcttgtga ttcagatcac aaagattcaa caaaccatca aaacaaaaat gtgacactac
      781 tcacatttaa atgccatttt tagcgaaaat cgccgccttg ttgctttttt acacaagcgt
      841 tttgtgatga acgtcacgtt aattacctct ctacccccta tatttatgtg attgatatca
      901 cacaaaaggc cgtcgactgg acagttaacc gattcagtgc cagatttcgc agtatctaca
      961 aggtccggct acctctgccg ccacattaac aaaaaacctc gggcttccag cctgcgcgac
     1021 agcaaacata agaaggggtg tttttatgtc atccgatatt aagatcaaag tgcaaagctt
     1081 tggtcgtttc ctcagcaaca tggtgatgcc aaatatcggc gcgtttatcg cgtggggtat
     1141 catcaccgcg ttatttattc caacagggtg gttaccgaac gagacgctgg cgaagctggt
     1201 cgggccgatg atcacttatc tcctgccgct gctgatcggt tataccggtg gtaagctggt
     1261 aggcggcgaa cgtggcggcg tagtcggtgc catcaccacc atgggcgtta tcgtcggcgc
     1321 agacatgccg atgttcctcg gttctatgat tgcaggtccg ctgggcggct ggtgcattaa
     1381 gcacttcgac cgctgggtag acggtaagat caaatccggt tttgagatgc tggtgaataa
     1441 cttctccgca ggcatcatcg ggatgatcct cgctattctg gcattcctcg gcattggccc
     1501 gattgttgaa gccctgtcca aaatgctggc tgcgggcgtt aacttcatgg ttgtccatga
     1561 catgctgccg ctggcgtcta tctttgttga accggcgaaa atcctgttcc tcaacaacgc
     1621 cattaaccac ggtatcttct cgccgctggg tattcagcag tcccatgaac tgggtaaatc
     1681 aatcttcttc ctgattgaag ctaacccagg tccaggtatg ggcgtgctgc tggcgtacat
     1741 gttctttggt cgtggtagcg ctaaacagtc tgcgggcggt gcggcaatca tccacttcct
     1801 gggtggtatc cacgaaatct acttcccgta tgtgctgatg aatccgcgtc tgatcctcgc
     1861 agtcatcctc ggcggtatga ctggcgtgtt cacgctgact atcctgggcg gtggtctggt
     1921 ttctccggca tctccgggtt ctatccttgc tgtactggcg atgacaccaa aaggtgctta
     1981 tttcgctaac atcgcgggtg tgtgtgcggc gatggctgtc tccttcgttg tctctgctat
     2041 tttgctgaaa accagcaaag tgaaagaaga agatgatatt gaagcagcaa ctcgtcgtat
     2101 gcaggacatg aaagctgagt ctaaaggcac atctccgctg tctgcgggcg atgtgactaa
     2161 cgacctgagc cacgtacgta aaatcatcgt tgcctgtgac gccggtatgg gttccagtgc
     2221 gatgggcgca ggcgtgctgc gtaagaaaat tcaggatgca ggtctgtcac agatttcggt
     2281 cactaacagc gcgatcaaca acctgccgcc agatgtggac ctcgtcatca ctcaccgtga
     2341 cctgaccgaa cgcgctatgc gccaggttcc gcaggcgcag catatttcgc tgaccaactt
     2401 cctcgacagc ggcctgtaca ccagcctaac cgaacgtctg gttgctgccc agcgccatac
     2461 tgaaaacgaa gtgaaagtga aagacagtct gaaagacagc tttgacgatt ccagtgctaa
     2521 cctgttcaaa ctgggcgcgg agaacatctt cctcggtcgc aaagcagcaa ccaaagaaga
     2581 agcgattcgt tttgctggcg agcagctggt gaaaggcggt tacgttgagc cggaatacgt
     2641 tcaggcgatg ctggatcgtg aaaaactgac cccgacttat ctgggtgagt ctatcgcggt
     2701 gccacacggt acggttgaag cgaaagatcg cgtactgaaa acgggcgtgg tgttctgcca
     2761 gtacccggaa ggcgtgcgct tcggtgaaga agaagatgac attgcccgtc tggtgattgg
     2821 tattgcagcc cgtaacaacg agcacattca ggttatcacc agcctgacca atgcactgga
     2881 tgatgagtcc gtcatcgagc gtctggcaca caccaccagc gtggatgaag tgctggaact
     2941 gttggcaggt cgtaagtaat ccaatcccac cctctccaca tggagaaggt ggggttaatt
     3001 gcctgatgcg ctacgcttat caggcctaca ggatgcatca cgatttgttg aatttgcacg
     3061 ttcttgtagg ccggataagg cgcttacgcc gcatccggcg ctgcccctct cctcacggag
     3121 agggtttggg tgagggaaaa gcctcacccc agccctctcg ggtaaaaaca ttgatgaagg
     3181 ttaatactat gaaagcatta cattttggcg caggtaatat cggtcgtggc tttatcggta
     3241 aactgctggc agacgcgggt atccaactga cgtttgccga tgtcaatcag gtggtacttg
     3301 atgccctgaa tgcccgtcat agctatcagg ttcatgtggt tggtgaaacc gagcaggtag
     3361 ataccgtttc cggcgtcaat gctgtcagca gcattggtga tgatgtcgtt gatctgattg
     3421 ctcaggtcga tttagtcact accgccgttg gcccggttgt gctggaacgt attgctccgg
     3481 ctatcgccaa agggctggtg aaacgcaaag aacaaggtaa tgaatccccg ctgaacatca
     3541 tcgcctgtga aaacatggta cgcggtacca cgcagctgaa aggccatgtg atgaacgccc
     3601 tgccggaaga cgccaaagcg tgggtagaag aacacgttgg ctttgtcgat tccgccgttg
     3661 accgcatcgt accgccttcg gcttcggcaa ctaacgatcc gctggaagtg acggtagaaa
     3721 ctttcagcga atggattgtc gataaaacgc agttcaaagg cgcactgccg aacatcccag
     3781 gcatggagtt aaccgacaac ctgatggcat ttgtcgaacg taaactcttc accctgaaca
     3841 cgggtcatgc tataaccgcg tacctcggaa aactggccgg tcatcagacc attcgtgacg
     3901 ctattctcga cgagaaaatc cgcgcggtgg taaaaggtgc gatggaagaa agtggtgcgg
     3961 tactgatcaa gcgctacggc tttgacgcag acaaacatgc ggcgtacatc cagaaaatcc
     4021 tcggtcgttt tgagaacccg tatctgaaag atgatgtaga gcgcgtaggc cgtcagccgc
     4081 tgcgtaaact gagtgctggc gaccgtctga tcaagccatt gctcggtacg ctggaataca
     4141 gccttccgca caagaatctg attcagggga ttgctggtgc aatgcacttc cgcagtgaag
     4201 atgatccgca ggctcaggaa ctggcagcac tgatcgctga caaaggtccg caggcggcgc
     4261 tggcacagat ttccgatctt gatgccaaca gcgaggttgt atccgaggcg gtaaccgctt
     4321 ataaagcaat gcaataatgg tggaccaggc gcaggacacc ctgcgcccga ataacagatt
     4381 gtcagatatg caggcaacaa tggaacaaac ccaggccttt gaaaaccgtg tgcttgagcg
     4441 tctgaatgct ggcaaaaccg tgcgaagctt tctgatcacc gccgtcgagc ttttgaccga
     4501 ggcggtaaat cttctggtgc ttcaggtatt ccgcaaagac gattacgcgg tgaagtatgc
     4561 tgtagaaccg ttactcgacg gcgacggtcc gctgggcgat ctttctgtgc gtttaaaact
     4621 catttacggg ttgggcgtca ttaaccgcca ggaatacgaa gatgcggagc tgctgatggc
     4681 attgcgtgaa gaactaaatc acgacggcaa cgagtacgcc tttaccgacg acgagatcct
     4741 tggacccttt ggtgaactgc actgcgtggc ggcgttacca ccgccgccac agtttgaacc
     4801 agcagactcc agtttgtatg caatgcaaat tcagcgctat caacaggctg tgcgatcaac
     4861 aatggtcctt tcactgactg agctgatttc cagaatcagc ttaaaaaaag cctttcaaaa
     4921 gtaagcaacg tctgcttact gcccctctac ctgcttcggc cgataaagcc gacgataata
     4981 ctccagacgt tgtagatata acggcacgct ttcatcgggt attccggacg gaatcgcgtt
     5041 acggggagga agttttttca gatactcccg gaacgcctgg cttgatgcca tgaaatctac
     5101 ggctttatcg ataagaagcg gaacgttttc acctaatttg cccatgatga tatctccgta
     5161 ttaccccgcg ccgggaatgc gcggccgcca attttagttt aggttcccgg taaagatgac
     5221 attaaggaaa acgtgctgaa tcctcaataa tcatccgctg ctgtttatta tcttttttct
     5281 atagatttct taattaatca ataaattgcg tagtttttaa gcatgttaat gattacgtta
     5341 cagaactatt gcaatgtcat cagttgtgaa caaagcacct ggtcgcgcat actaggggct
     5401 ataaatttat ctttatcaga agccatcaca tgaaagaagt cgaaaaaaac gaaatcaaac
     5461 gtctcagcga tcgtctggac gccatccgcc accagcaggc cgatctgtcg ctggttgaag
     5521 ccgcagacaa atatgccgag ctggaaaaag agaaagccac gctggaagca gaaattgctc
     5581 gcctgcgtga agttcatagc caaaaactga gtaaagaagc acagaagctg atgaagatgc
     5641 cgtttcagcg cgcaattacc aaaaaagagc aggctgatat gggcaagctg aagaaaagtg
     5701 ttcgcggact ggtggttgtg cacccaatga ccgcgctggg ccgtgaaatg ggcctgcagg
     5761 agatgactgg attctctaag actgcgtttt aagcgcctca gacgactggt tcgatcatat
     5821 caacccgcat ctggcttgcc ttagctggtc aatgcgggga ctctcctgtc tactcgccta
     5881 attaccaaac aattcactcc ctttacgtcg atttaccgct ggttgatatt ctgaaaatgc
     5941 atttttaccg aaatagccac ctataaacaa taaaaatata tagaagat
//
LOCUS       DAZOZY010000225         5931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZOZY010000225 DAZOZY010000000
VERSION     DAZOZY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.228"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHURQ_003752"
     tRNA            292..368
                     /locus_tag="ACHURQ_003752"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHURQ_003753"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHURQ_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR0825425.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHURQ_003754"
     CDS             complement(1357..2247)
                     /locus_tag="ACHURQ_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR0825426.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHURQ_003755"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHURQ_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR0825427.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHURQ_003756"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHURQ_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR0825428.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1561 c         1549 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAZOZY010000226         5916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZOZY010000226 DAZOZY010000000
VERSION     DAZOZY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.229"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            109..555
                     /locus_tag="ACHURQ_003757"
     CDS             109..555
                     /locus_tag="ACHURQ_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR0825429.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            566..2017
                     /locus_tag="ACHURQ_003758"
     CDS             566..2017
                     /locus_tag="ACHURQ_003758"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR0825430.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2007..3077
                     /locus_tag="ACHURQ_003759"
     CDS             2007..3077
                     /locus_tag="ACHURQ_003759"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR0825431.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3077..4825
                     /locus_tag="ACHURQ_003760"
     CDS             3077..4825
                     /locus_tag="ACHURQ_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR0825432.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4875..5915)
                     /locus_tag="ACHURQ_003761"
     CDS             complement(4875..5915)
                     /locus_tag="ACHURQ_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR0825433.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFK
                     QGPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
BASE COUNT         1367 a         1499 c         1508 g         1542 t
ORIGIN      
        1 acctttcact ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc atcacttaat
       61 cgctccacca ttacaattga ttaaaatata ttttatggag tccgattaat ggcagctctt
      121 actgcaagct gtattgacct gaatattcag ggcaatggcg cttattccgt tctgaagcag
      181 ttggcgacaa tagcgttaca aaacggtttt atcaccgact cacaccagtt cctgcaaacc
      241 ctgctgctgc gcgaaaaaat gcactctact ggatttggtt ccggtgtcgc cgtgccgcac
      301 ggtaaaagcg cctgcgttaa acaaccgttt gtattattcg cccgcaaagc gcaggctatt
      361 gactggaaag ccagcgatgg cgaagacgtc aattgctgga tctgcctcgg cgtgccgcaa
      421 agcggcgaag aggatcaggt caaaatcatc ggcacactgt gtcgcaaaat tatccaccag
      481 gattttattc atcaactgca acagggcgac gccgaccagg tgcttgcctt gttaaatcaa
      541 accctcagct cataaggaag tggcgatgga gtcatcctta tgtattgtcg cgatcaccaa
      601 ctgccccgcc gggatcgctc acacctacat ggtggcggaa gccctggaac agaaagcccg
      661 ttctctcggc cataccataa aagtggaaac tcaagggtcc agtggcgttg aaaaccgctt
      721 atccagcgaa gagattgccg ctgccgatta cgtcattctc gctaccgggc gtggcctgag
      781 cggtgatgat cgcgcgcggt ttgccgggaa gaaagtttat gagattgcca tctcccaggc
      841 gttgaaaaat atcgaccaga ttttcagcga attaccgaca aactcgcagc tttttgccgc
      901 agatagcggc gtgaagcttg gcaaacagga agtgcaaagc ggcagcgtga tgagccacct
      961 gatggcgggc gtctctgcgg cgctgccgtt tgtcatcggc ggtggtatcc tggtggcgct
     1021 tgccaacatg ctggtgcagt tcggtttgcc gtataccgat atgtcgaaag gcgcgccgtc
     1081 gtttacctgg gtagttgaat ccatcggtta ccttggcttc acctttatga tccccatcat
     1141 gggcgcttat attgcctcgt cgattgccga taaaccggct ttcgctccgg cgtttctggt
     1201 ttgctatctg gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg gattcctcgg
     1261 cgcggtggtg ctggggctgg cgattggcta tttcgtcttc tggttccgca aagtgcgcct
     1321 tggcaaagca ttacaaccgc tgctcggttc aatgctgatc ccgttcgtta ctctgctggt
     1381 tttcggcgtg ctcacttact acgttatcgg gccggtgatg tccgacctca tgggcgggct
     1441 gctccacttc ctgaatacca ttcctccatc aatgaagttt gcggcagcgt ttctggtcgg
     1501 tgcgatgctg gcgttcgata tgggcggccc catcaataaa accgcctggt tcttctgctt
     1561 ctcactgctg gaaaaacaca tttatgactg gtacgccatc gtcggcgttg tcgcgttgat
     1621 gccgcccgtt gcggcgggtc ttgcgacgtt cattgcgccc aaactgttta cccgacagga
     1681 aaaagaggct gccagtagtg ccattgtggt cggtgccaca gtcgcaaccg aaccggctat
     1741 accttacgct ctggcagctc cgttgccgat gattaccgcc aatacgctgg cgggtggcat
     1801 taccggcgtg ctggtgatcg ctttcggaat caaacgtctg gcacctggtt tgggtatctt
     1861 tgacccgctg atcggcctga tgtcgccggt gggttcgttt tatctggtgc tagcgattgg
     1921 cctcgcgctg aacatttcat tcattatcgt cctgaaagga ttgtggttac gccgcaaagc
     1981 gaaagccgcg caacaggagc ttgtccatga acattgagtt actgcaacag ttgtgcgaag
     2041 ccagcgccgt cagcggcgat gaacaggaag ttcgcgacat tctgataaac acgctggaac
     2101 cttgcgttaa tgagatcacc tttgatggtc tgggcagctt tgttgcccgt aaggggaata
     2161 aaggtccaaa agttgccgtt gtcgggcata tggatgaagt cggctttatg gtcacccaca
     2221 tcgacgagag cggttttctg cgctttacca ccattggcgg ctggtggaat cagtcaatgc
     2281 tcaaccaccg ggtaaccata cgcacacaca agggatttaa aatccctggt gtgattggtt
     2341 ccgtcgcacc tcatgcgtta acggaaaagc aaaagcaaca gccgctgtca tttgatgaga
     2401 tgttcattga tattggcgcg aacagtcgcg atgaggtgga aaaacgcggt gtggcgatgg
     2461 gcgattttat tagcccggaa gccaattttg cctgctgggg cgaagataaa gtggtcggca
     2521 aggcgctgga taaccgcatc ggctgcgcaa tgatggctga actattgcag acggtgaata
     2581 atcccgaaat tacgctgtat ggcgtcggca gcgtggaaga agaagttggg ctacgcgggg
     2641 cacaaacctc ggcggaacac attaaaccgg atgtggtgat tgtgctggat accgctgtcg
     2701 caggtgatgt tccgggcatt gataacatta aatacccgct gaaactgggc caggggccgg
     2761 ggctgatgct gtttgacaag cgctacttcc ccaaccagaa actggtggcg gcgttaaaaa
     2821 gctgtgccgc acataacgat ttaccgctgc aattttccac catgaaaacc ggtgcgacgg
     2881 atggcgggcg ctacaacgtg atgggcggcg ggcgtccggt tgttgcgctg tgcctgccaa
     2941 cccgctatct gcacgctaac agcggcatga tttcaaaagc cgattacgat gctctgctca
     3001 cgttgatacg ggattttctg accaccttaa ccgcggagaa agtcaacgcg tttagccagt
     3061 tccgtcaggt ggattaatgc taaacgaacg ccagttaaag attgtcgatc tgctggagca
     3121 acagccgcgc acgcctggtg agctggcgca acagactggc gtttcaggca ggaccatcct
     3181 gcgtgatatt gactatctca actttaccct taacggcaaa gcccgcattt tcgccagtgg
     3241 cagtgcgggc tatcagctgg aaatcttcga gcgccgcagc ttttttcagt tgctgcaaaa
     3301 gcacgataac gacgatcggc tgctggcgct gttattactg aataccttta ctccccgtgc
     3361 gcaactcgcc tcggcgctta atttgccaga aacctgggta gcagagcgtc tgccccggtt
     3421 aaaacagcgt tatgaacgca cttgttgcct ggccagtcgc cccggtttgg gccatttcat
     3481 tgatgagaca gaagagaaac gcgttatctt gctggcgaac ttgctgcgca aagatccgtt
     3541 tttaattccg ctggcgggcg taacacgaga caattttcag catttatcca cggcctgcga
     3601 caaccaacac cgctggccgc tcatgcaggg tgattatctc tccagcctga ttctggcgat
     3661 ttacgccctg cgtaatcaac tgaccgatga gtggccgcaa tatcccggta acgagataaa
     3721 acaaatcgtt gaacagagcg gcatgtttct tggtgataac gctgtaagaa ccctgacagg
     3781 tttgatagag aaacagcatc agcaagcgca ggtaatttca gccgatcatg tgctggggtt
     3841 gctgcaaagg gtgccaggca tcgcgtcatt gaatattatt gatacgcagc tggttgagaa
     3901 tattaccggg catttattac gttgccttgc cgcaccggtg tggattgccg agcaccgcca
     3961 aagcagcatg aataacctga aagccgcctg gcctgcggcc tttgatatga gtctgcactt
     4021 tattacgcta ctgcgtgaac agctcgatat tccccttttc gatagcgatc tgatcggttt
     4081 gtattttgcc tgtgcgctgg aacggcatca aaacgaacgc cagccgatca ttttgctctc
     4141 ggaccagaac gcgattgcca ctattaatca gctcgccatt gagcgcgatg ttttacattg
     4201 tcgggtgatt attgcccgta gcttaagcga gcttattgcc attcgcgaag agattgagcc
     4261 gttattgatc attaacaaca gccattattt actggatgac gcggtaaata attacatcac
     4321 cgtaaaaaat atcattacgg ctgccgatat cgaacaaata aaacactttc tggcgacggc
     4381 atttattcgc caacagccgg aacgcttttt ctctgccccc ggaagttttc attattcgaa
     4441 tgtacgcggt gaaagctggc aacatattac ccggcaaatt tgtgcgcaat tagtggcaca
     4501 acaccatatt accgccgatg aagcgcaacg gatcatcgcc cgcgaaggcg aaggtgaaaa
     4561 cctgattgtt aatcgcctcg ccatcccaca ttgctggagc gaacaggagc gacgttttcg
     4621 tggatttttt attaccctcg cccaaccagt tgaggtgaat aacgaagtca ttaaccatgt
     4681 cttgatcgcc tgcgccgccg ccgatgcgcg tcatgagcta aaaatattta gctatctggc
     4741 aagcgtattg tgtcagcatc cggcagaagt tattgccggg ttaacaggat atgaggcatt
     4801 tatggagtta cttcacaagg ggtgaactgt gtcggatgcg ccgcatccga cactgcattt
     4861 atcatcaaaa acgtttaacg caggctatta tagtcatcaa ccatttcgtt gtactcgcgt
     4921 aacgctcgtg gaaatgagtc ttcgaccatc cagcttgtgt ttgcatcctg aagatgctct
     4981 ttatcccagt cagagtacgg gactttatct ctgacgcggc gaacatattt tttagtctcg
     5041 agttgatact ggtctgccga attaatgaac gagaaattca taccgccttt atccgcttct
     5101 ttggcctgcg ctaccagcgt ttccagttca gagactttct tcattgccgc atcaacatca
     5161 aacttctctt gcgaaatcag gttattaatt tgtttcgctg aaatcatgac tgccagagag
     5221 tagtagtgga atgttttccc ttctctttct tcaatatttt tcagttccgc aagctggcgc
     5281 ttatcattaa tttcctgaat cgccgcatga taagattctg ctacaggttc aaaggattcc
     5341 agatttttta agaatgtctg gtgcagcgtt ttccctttcg caaacgcatc gtctttataa
     5401 ttttcctggg tgtaatattt atccatttcg ttaatggtat tacccaacgc cacggcagca
     5461 tcaatataac tcaccgccac gccatcaata ggttgcagcg caggcgttaa cgccacagca
     5521 ctttttacgc ctttttcaca ctcagcgagg ttggattcac taatgccgta gatgccataa
     5581 acagtactct ctttaccggt aggcccctgt ttaaaatctt tcagccagtc agcataacgc
     5641 gccagactgt tctgtacagg gatctgtaac ttgttgtagc acttgatata gacatccatt
     5701 ttttcgtcga aaaccgcttg ttcatcgacc accggttgcg caggtgtttc aggtttagct
     5761 tctgcttttg caacgggagc ttcaggagct ggtgcggcag gttgactatt ggcaggcggg
     5821 ggcgtttctg tttcggcttt tttatcatca caacccgtca gaccgagggc catgatggcg
     5881 actataatag cggaagataa taaattcctt ttcatt
//
LOCUS       DAZOZY010000227         5915 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZOZY010000227 DAZOZY010000000
VERSION     DAZOZY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.230"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            86..1018
                     /locus_tag="ACHURQ_003762"
     CDS             86..1018
                     /locus_tag="ACHURQ_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR0825434.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(1060..2148)
                     /locus_tag="ACHURQ_003763"
     CDS             complement(1060..2148)
                     /locus_tag="ACHURQ_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR0825435.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(2290..>5915)
                     /gene="ehaA"
                     /locus_tag="ACHURQ_003764"
     CDS             complement(2290..>5915)
                     /gene="ehaA"
                     /locus_tag="ACHURQ_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR0825436.1"
                     /translation="GFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKGT
                     SRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQN
                     SDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEKK
                     ITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGITQ
                     NIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVASG
                     AVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFAY
                     TDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVDF
                     GALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNSA
                     IKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVNN
                     DGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVSL
                     SNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSADS
                     LLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLVS
                     HAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVLV
                     VKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIEL
                     IHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPNP
                     EPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYYT
                     DMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWHV
                     GVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSWF
                     DNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGVK
                     ADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSKD
                     FSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGIK
                     WQF"
BASE COUNT         1403 a         1595 c         1309 g         1608 t
ORIGIN      
        1 ttcaaaaatg attatctatc tttaaccgat acctctacag tcatagctgg agaaattcat
       61 agtagataat ggcgagaatg gtttaatgaa ctcaattttt accgaagaga atctgttggc
      121 atttactacc gctgcgcgtt ttggcagctt cagtaaagcc gcagaagagt tgggtttaac
      181 cacttccgcc attagctaca ccatcaagcg tatggagacg gggctggatg tggtgctgtt
      241 cactcgcagt acccgcagca ttgagttaac ggagtccggg cgttattttt tccgcaaagc
      301 taccgacctg ctgaatgatt tttatgccat caaacgcagt atcgatacta tttcgcaggg
      361 catagaagcg cgtgtgcgca tttgtattaa tcagctgctt tatacgccca aacacaccgc
      421 acgcttgtta caggtgctga aaaagcagtt tcctacctgc cagattaccg tcaccaccga
      481 agtgtataac ggcgtctggg atgcgattat taataatcag gccaatatcg ccatcggcgc
      541 acccgataca ctactggacg gcggcggtat tgattacact gaaattggcg cgattcgctg
      601 ggcttttgct atcgccccgg atcacccgtt agcctttgtg ccggaaccca tcgccgaaag
      661 ccaactgcgt ctctacccta atattatggt ggaggacacc gcgcatacga ttaataaaaa
      721 agtgggctgg ttgctgcacg ggcaggagtc aattctggtg ccagatttca acaccaaatg
      781 tcagtgccag attttaggtg aaggaattgg ttttttgccg gattacatgg tccgtgaggc
      841 gatgacgcaa tccctgctgg ttacccgaca aatccataat ccgcgccagg attcgcgcat
      901 gttactggcg actcagcatt ctgctacggg tcaggtgacg cagtggatta aaaaacaatt
      961 tgcgccaaat ggcatattaa cggggatcta tcaggattta ctgcatcggg agaattaatc
     1021 gctgccaacc tcccgatgcg ctgtgaatag cggtttacat caaccacctg ctttcattac
     1081 ccattcagag ataaatttat tacccggtac tggcggagag tacaaatagc cctgcaaaaa
     1141 gtgaacgcct ttaccgatca ttaaatccgc ctgctcctgg gtttctaccc cttccgccac
     1201 gatactcaga ccaggcttac gcgctagttc gacaatattg tccacaatat gaccggagat
     1261 ttcgtcgaca ctcgccattt gcacaaatga cttatcgatc ttaataaaat cgaccgggaa
     1321 cgcctgcaag taacgatagg tcgcataacc cgtaccaaag tcatccagcg caaaggtaat
     1381 gttgtgctga tgaaggctgt caaatatcgc tctggcttct ggcgttaccg gaatagggtt
     1441 acgttccgtt agctcgagaa ccagcttgat tttatcgtta cctaatttct taaccaggtt
     1501 cagacactct ttttcaaatc ccgctgccaa aaaacaaccc gccgagacgt tgatgccaat
     1561 atggaaattg tccggcagca aatgttttac cggcatcaga atatccgcag tctgtttcat
     1621 cagttggcgg gtcattatga caataagacc ggatgactcc gccagaggaa taaactgatc
     1681 cggtgggata attcccgttt gtggatgttc ccagcggaca agtacctcac agcccgtcag
     1741 taccccagtc tgcgcgcaga aaaccggttg gatccacggt ttgaattcgt ggttttccaa
     1801 cgccagactg atggcttcag gcgtgacgat atggtgacaa cggttatcag ggatataaaa
     1861 attattttta tttcccgtgc cactgatgac ttgcggatga tattgaaaaa agatatcccg
     1921 ccagaaggta atatcactct ggatgcctag tttttcatac agctgcttct tttgatgtga
     1981 aattgtcttt gcactgcgat ttctgtactg tgagatttct gtaaccgaca ttccctccat
     2041 tagcagcttg agtaaaagcc gttctttttc ggacacgata ccaggcaacg tcaaattcac
     2101 cgttgccccg aactcttcca aaaaaacact caaatcatgc gaagccattg aacacacctt
     2161 gattaaatct tgtgcgccag ctaaatctgc gagtcttcac tctgactgat aaagctggct
     2221 gcaagaaaat gacacagaga gccgaattat ctcccgttcg gctctctctc cttttactca
     2281 ttattcagat cagaattgcc acttaatgcc aaccattgca gaggtgtcat tatatcccct
     2341 atcggcaacc tgaacgccca cattccccca gacattcagg ttggcattta gctgtccttc
     2401 caccccggtt tttatctcag caatatttcg ggctccatcc tgagtgacgg acacgccatc
     2461 catactggtg ctgaaatcct tactgttatg cagccagttc acttctacaa acggctggaa
     2521 ctcgcgggat ttaccgtcat ccattttatg gtggctcttc agccaggttt ttacgccaag
     2581 tcgggtttga acattgccat caccgttgct atgaacgagg gttccgttgc tttcgcggtg
     2641 cttatcggct ttgactccca tccaggtaac ctgtgcttgc ggctgaacat accattcatt
     2701 acgcgttccc tggctgccat taaattcagc taatttgtgt ttgtatccag cttccagtga
     2761 agcggtaaat ccttttgatt tataggattc actttgtaag tcatcccctt tcactgtgtt
     2821 atcaaaccag ctgtactgcg cccaactgtc gagatacgcg ccattacgcg actcgtcatc
     2881 ggcataccag gtggcataga ggccggtgct atatccgttc acactcgctt ttgcacgata
     2941 accggttcgc gaggaaatgg ttttgctgtc gctgttgcca tatcccgcca tgaccccaac
     3001 atgccagctg tcgctgccgt tttggctcca ctgcgcgaca tcgcctccca gttgcagaac
     3061 atagcgattg ctttgggttt tcagctggcc gctgccatca cgccatttat tatgaccacc
     3121 ttcatggcgc atccacatag tggtttgttt ctgctcaccc gtcaccatgt cggtatagta
     3181 cgtattaccc agacgctcat gcagacgcgt ggtgaacatt gtattcgctg ctgcaaggtt
     3241 cgcaatgtag ctacccgcct ccggtcgcag gtcattatcc acattcagat ccggacccgg
     3301 cgtaggtgtc gggttagggt tcggctctgg attcgggttt ggctccggat tcggcatcgg
     3361 gtctggctcc ggctgcagtt caggagaatc actgccgctg gtcagatacc agttaccact
     3421 atttgcccct tgtccacgcg cgagagtgta gtcatacgcc cccgcaacga tacgaccagc
     3481 ctgaacaaat tcgccctcag acttaccgtc tacatggata agttcgatac cgttaagggt
     3541 tttcgcacct gtaccgccag cgttattcac cgtaacggca gtcgtgccgc tagtatcgcc
     3601 ctcgacaacc aatttatcgg ttactgagtc atcgccattc agtaccgtat tcattactag
     3661 ttgaccgtta ttaccgtggt agttcccctt caccactaac acattgccag cttgctgtcc
     3721 tgacattccg gtcatcagcg taccgttgtt ggtcagattg ccgcccaggg tgtaattcac
     3781 agacgcagcc gctctttttt cagcgctatt attcaacgcc agggtaccgt tgttctcaac
     3841 gtttgcgcta cccaatccct gcgcatgact taccaggaca ttagccgaat tagcaatgtt
     3901 cacattcgct gtcaggttgc tgttatcgcc cgcgatgtcc agcgtgccgc cctgaatatt
     3961 cagctctccg ctccccgtta acgaaccggt tgaagtgccc ccgttggtaa ccgtcagact
     4021 gccgccattt aagctcagca gtgaatcggc gctgccattg agcgtgccca cggtctggct
     4081 gtgaccattc atatccagtt cagtctctgc cgccagacgc agttcatggg tattacccaa
     4141 cacgttatcg ttattcaaca aaagcgtccc actgcgcaga tcggtaacac cggtatagtc
     4201 gttgtctttg ttagaaagcg atacggtctg acccttctgg ctgctgaatg ccaggtcacc
     4261 actgccggta atctttgcgc tgagatcggc ggcattctcg cttttaccgt tagagctcag
     4321 caccagagca tcgctgtcgg tagcgtgtaa atcaagctgg gtcaggccgt aaccgatata
     4381 cagaccgtca ttgtttaccc cactcgtcag acgatagtcg taagtgccga cggccgcttt
     4441 ttgcccattc tgctgaatat cacgttgggc actgctggag aggatttgcc catttttatc
     4501 ctgcaattgc agattgcccg catcgcccag aacttccgca ccttgcgcat caaccagctt
     4561 aatggcgctg ttaccatcat cgacttcagt gagcgataac gcagagtcaa tatcacgaga
     4621 tactgagcgg acaacgtcac tgtcagaaac ctgaatcaca ccctcgccgc gcaaatccag
     4681 cgttttacta acgttgaccg tcccttctgt catctgtgca cctgctgtca gggcaccaaa
     4741 atcaacggtt ccgccggaga atcccaaagt gctaatggag tgagcgccct tacccactgt
     4801 taccaggctt ccctgacccg cagtgatccc gcctctctgc agaaactcag cattcttttc
     4861 caggttaaat gtcgtgttct ggaacgccag tgaaccctgg aaccagtctg tataagcaaa
     4921 ctcatcgaag gagaattcgt gattccccat gtttatttca acaatacctc taccttgaag
     4981 atcgttatca aatacccaat ctttattggc attgatttgc agtttgccat caacatccag
     5041 gtccgcacgg ccgatgttct tgtcttcaga aataaccgcg acagcaccag aagcgacatc
     5101 aatatatccg ctgaatgcat agttattgcc ggtcagcgcc actgtcccac tgtcgatact
     5161 tattgtaccg ttgccggaaa tcttattggc aaaggtcatg ctatcgtcag ccttcaggtg
     5221 taatttcccg cggttgttga tttctgcggt acctatattt tgggtgatcc ctaaagcgcc
     5281 attttgctca attttgactt taccgctaaa ggcggagtta tccccggtta agtaagtcgt
     5341 accattgagc gcattgatat agcccaggcc ctcgctgtca ctgacaatat ccgcggaaat
     5401 agtcgcgttt ttatccgtgt ggtttaaggt gattttcttc tcggatgtct ttgcccacac
     5461 aaactcaatt ttttcggcat caataatccc tgcagccttc gctgcgcttc cttcctgtgc
     5521 acctaacgct aactcagagt tggtgcttaa actaatagtc ggcgcagaga ttttaccacc
     5581 ttccgaaaca gcaagtgttg tatcggagtt ttgatcataa taacccaggc taattttctc
     5641 actggcatta acttcaccgc cgttattaac tgacaggtac tgattaccac cgtaactgcc
     5701 gtaaacgccc ataaagaagg ttttagaatt tacctggctg ccttcaccac tgacatttcc
     5761 ctgcaaatag gtcagacggc tggtgccctt aacatggatt ttgccgccgt tggagacatt
     5821 aaaatagccg cgatcgtgtc tcatgcctga gtttgactca tacaaatcat cagcatataa
     5881 ctcgctgcca gcattggaga cattcaggaa ccccg
//
LOCUS       DAZOZY010000228         5899 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZOZY010000228 DAZOZY010000000
VERSION     DAZOZY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.231"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHURQ_003765"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHURQ_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHURQ_003766"
     CDS             2192..3328
                     /locus_tag="ACHURQ_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR0825437.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHURQ_003767"
     CDS             3325..5325
                     /locus_tag="ACHURQ_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR0825438.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5450..>5899
                     /locus_tag="ACHURQ_003768"
     CDS             5450..>5899
                     /locus_tag="ACHURQ_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR0825439.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFK"
BASE COUNT         1364 a         1569 c         1607 g         1359 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgacacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgctttttac
     5341 taagagttct actccagttc cggactgctc acgccacggt attaggcata tcctatatag
     5401 cccctggtga gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt
     5461 caataagctg ttttccctcg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg
     5521 tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac
     5581 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc
     5641 ctccattggt gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa
     5701 atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga
     5761 aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
     5821 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
     5881 gaaagccgct ggttttaaa
//
LOCUS       DAZOZY010000229         5865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZOZY010000229 DAZOZY010000000
VERSION     DAZOZY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.232"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..560)
                     /locus_tag="ACHURQ_003769"
     CDS             complement(<1..560)
                     /locus_tag="ACHURQ_003769"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR0825440.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
     gene            complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHURQ_003770"
     CDS             complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHURQ_003770"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR0825441.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHURQ_003771"
     CDS             complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHURQ_003771"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR0825442.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2371..3636)
                     /locus_tag="ACHURQ_003772"
     CDS             complement(2371..3636)
                     /locus_tag="ACHURQ_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0825443.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3863..5077)
                     /locus_tag="ACHURQ_003773"
     CDS             complement(3863..5077)
                     /locus_tag="ACHURQ_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR0825444.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5177..5533)
                     /locus_tag="ACHURQ_003774"
     CDS             complement(5177..5533)
                     /locus_tag="ACHURQ_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR0825445.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5669..5776)
                     /gene="rprA"
                     /locus_tag="ACHURQ_003775"
     ncRNA           complement(5669..5776)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHURQ_003775"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1330 g         1506 t
ORIGIN      
        1 cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac
       61 tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc ttgctggcgt
      121 ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg
      181 gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt
      241 tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc ccatttcacc
      301 agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag ccctgtcgga
      361 ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc aagagctgta
      421 attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg
      481 tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga
      541 ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc ctacgccaga
      601 tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt aaatatattt
      661 tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
      721 tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag gcttttttta
      781 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
      841 tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc atctccaggg
      901 tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
      961 tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
     1021 ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga
     1081 cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata tcaaccaggc
     1141 cgctgtcgat ggctgcacga ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc
     1201 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtttcacgga
     1261 gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag tggtccacac
     1321 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgcggg
     1381 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgcacca atgacgagat
     1441 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
     1501 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataacgtga
     1561 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caacctgctt
     1621 gttgtgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagca
     1681 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg gggttcatac
     1741 ctacttttgt gccattggtt aaaatgtcgg cggaagccag gacttcagca aaggcttgct
     1801 gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga
     1861 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
     1921 cttcaattgc cccctgcgcg ccaatcgctg ttgaggcacc tccggcccct aacagcacca
     1981 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
     2041 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcacccacca
     2101 gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
     2161 acacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg ggaaagctat
     2221 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
     2281 tctgcatttc aggcgataaa ctgtggcgga taggataagc catcaaccca atcaattcgt
     2341 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
     2401 ggtgctaaat ttgcgttcgc caaaccgcac atcattctca ggaataataa aaacgcggta
     2461 atagcggcta aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
     2521 aacaatgata tattgcaggc cgatgttcga cagataaccg gtaatcagcg gaataacaaa
     2581 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttggggaa
     2641 aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg agaaaccgat
     2701 aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa tggcagctgt
     2761 tattgttgcc agcgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
     2821 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
     2881 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
     2941 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
     3001 ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg cgtccaccaa
     3061 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca gaatacccgg
     3121 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
     3181 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccagaggc
     3241 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
     3301 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
     3361 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
     3421 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
     3481 ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgtca tattttgggc
     3541 aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa gaacagccag
     3601 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
     3661 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     3721 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3781 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3841 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3901 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3961 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     4021 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
     4081 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     4141 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     4201 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     4261 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     4321 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     4381 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     4441 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     4501 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     4561 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     4621 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     4681 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     4741 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     4801 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     4861 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     4921 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4981 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     5041 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     5101 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     5161 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     5221 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     5281 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     5341 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     5401 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     5461 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     5521 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     5581 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     5641 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     5701 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5761 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5821 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctacc
//
LOCUS       DAZOZY010000230         5778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZOZY010000230 DAZOZY010000000
VERSION     DAZOZY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.233"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..661)
                     /locus_tag="ACHURQ_003776"
     CDS             complement(<1..661)
                     /locus_tag="ACHURQ_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0825446.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
     gene            761..1351
                     /locus_tag="ACHURQ_003777"
     CDS             761..1351
                     /locus_tag="ACHURQ_003777"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR0825447.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHURQ_003778"
     CDS             complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHURQ_003778"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR0825448.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHURQ_003779"
     CDS             complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHURQ_003779"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR0825449.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHURQ_003780"
     CDS             complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHURQ_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR0825450.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1505 a         1348 c         1424 g         1501 t
ORIGIN      
        1 ccagaatgat attccctgcg acagcaattt cctgctcctt tgcaaattgc cagtggaaag
       61 gtttgccgct gggataacga aatacaacac attgatgatt aagcagatca tgaggatggc
      121 ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggtg
      181 ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt
      241 caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg actttaatat
      301 caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc
      361 gggcggcatt gattttgagc gttcctgttg gtgtcagacg aaaatcattc atctcatcta
      421 acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt
      481 ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca
      541 gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac
      601 gaaagctttg attacgggcc acagcaaaga aagtggcaaa gtcagacata ttcatccgca
      661 ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacatgat tatgccgcct
      721 gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg aacgtattta
      781 tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg atgcgatcca
      841 tcagcgggca atggagcgga aaatacaggt gacggaactt gatttatatc ggcgtaattt
      901 caacccggtg atgacgccgg aagatgaacc agactggaag aatatggata aacgttattc
      961 tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag tggtggtttt
     1021 tcctgtctgg tggtacagct tcccggcaat gctaaaagga tatattgaca gagtatggaa
     1081 taatgggctg gcttatggag atgggcagaa attaccattc aataaagttc gttgggtggc
     1141 gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa atataagcga
     1201 ttatttaaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca ctttcttgtg
     1261 taatacagtg gtattcgatg gggaagaact tcacgcgagc tattatcagt cgttattatc
     1321 tcaggtacgg gatatggtag atgcactata agatgtgtta aaaacgctgt agcagaatga
     1381 agtgcggaat aaaaaagcgg caactcaata aagttgccgc tttacgggga aattagaaca
     1441 ttaccttatg accgtactgc tcaagaatgc ctttcacgcg ttccatggtc tctttcttcg
     1501 gtggtttaac accgtcgagt ttgtactctt cacccattgc cacccatttg tgtttgccca
     1561 gctcgtggta ggggagaagc tcgattttct caacgttgcc catatcacgg gtaaattcac
     1621 cgaggcgatg cgctgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc
     1681 acaccttcac atttttgttc gccagatatt tggcgaactc cagcgtgcgg tgattggaaa
     1741 ctccaaccag attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt
     1801 cggttacttc cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt
     1861 ccagacaggt atgaatgcct tcttttttgc aggcgcggaa ccagtcacga acaaactcag
     1921 cttgtaggat tgcctcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt
     1981 ggcgataggt caccacctct ttcattaaat cttcaacggt aacttcttta ccgccatgcg
     2041 tatcccaggt gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg
     2101 tgataaagcg aatacccggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac
     2161 caataactga cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc
     2221 agctcgaagg ctacgtcgag tctgttttgg cagtcacctt agagtataga tagctgacaa
     2281 aaaaggctct cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta
     2341 ctgtacgatt tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc
     2401 ctgctgctgt tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt
     2461 cagctgcgga tatttttccg ggttttccat cgcgtcgagc agcatttcac ggttcatcac
     2521 gttaacgttc aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat
     2581 cagaccagcc aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat
     2641 agagaaggtg taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt
     2701 cagagaggct actgcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa
     2761 cggcgcgcca gcacgacgac cgtctggggt gttacccgtt ttcttaccat acacaacgtt
     2821 agaagtgatg gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg
     2881 aattttcttc atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcgtt
     2941 gttaccaaac tgcgggtatt cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc
     3001 acgaatcggt ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag
     3061 accagcgata ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga
     3121 ggcttcgtag ctgtacttgt cgtgcatgta gtggatgatg tttagtgcag tgatgtactg
     3181 tttagccagc cagtccatga agtgatccat gcgctccatc acttcatcat agttcaggac
     3241 atcgcctttg atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc
     3301 gccgttgatt gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat
     3361 ttgtttacca acgatcatcg ggcttacgca gcaagcaata gcgtagtcat cgttgttgaa
     3421 gtccggacgc atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt
     3481 agcggcgaat ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg
     3541 ttccggagac ggacccatgg tgtacagggt gttcaggaaa cggaagctgt ttttggtaac
     3601 cagggtacga ccgtcgaggc ccataccacc gatagattcg gttgcccaga tcgggtcgcc
     3661 agagaacagt tcatcgtatt ccggagtacg caggaagcga accatacgca gtttcatgac
     3721 caggtggtca accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg
     3781 ttcgatgtac acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg
     3841 agacttaaca gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt
     3901 agccggacca gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc
     3961 gcggtgctgt tcagcgattt cttcgcgcag acggatagtc tgttccaagt ttacgccgtt
     4021 ttccagatca gcctgcagag aagtgaactg tgccagtttg tctttcatca ggtagtcgat
     4081 accgtacagc gcaacgcgac ggtagtcacc gatgatacgg ccacggccat atgcatctgg
     4141 cagaccggtc agaacaccag atttacggca acgcaggatg tccggagtgt aaacgtcgaa
     4201 cacgccctgg ttgtgagttt tacggtattc agtgaagatt tttttgatca tcggatccag
     4261 ttcgcggttg tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag
     4321 agcacgtttc agcggagctt cagtctgcag accaacgatt ttctcaagct gcttgttgat
     4381 gtagccagcg tcgtgagagg tgatggtgga agcaacagcg gtgtcaaagt caactggcgc
     4441 gtgagtgcgg ttttccagtt taacgccttc cattactttg tcccacaggg tggtggtcgc
     4501 ttcagtagcg ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat
     4561 gaagtcacgg acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt
     4621 ggctaacttt tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt
     4681 tactgcgtac ttcgacaacc attaatggtg gtcgttttca cgcaggtaaa tgacccagta
     4741 tgtcaaccca accaacaaac caccaccgat aatgttgccg atcgtaaccg gaatcaggtt
     4801 atcagtgatg aaattcatca cggtcaggtg agaaaaattt tccggtgcag aaccgactgc
     4861 ggtccaaaat tccggggatg cgaagtcgcg gattacaata cccatcggga tcataaacat
     4921 gtttgcgata ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccataat
     4981 gaacgctttg tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat
     5041 caggtttgcc aggataccaa gacagacggc ctcaataaaa gtatggtgca ctttgtggtc
     5101 ggcggtttgt aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag
     5161 ccacattaaa agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca
     5221 gtttttcgcc aactgacccc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac
     5281 ggtggaagta aagagatcgg ctccgcagac aacacaaaga atcagcccca gagagaagca
     5341 aatgccgcca accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt
     5401 gatatagaag acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt
     5461 cttaagcgga tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc
     5521 agcaggaagt aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag
     5581 tcggcgacga gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc
     5641 ctggcttata ggcccgtaac tcgcatggtt tttatgcaga tacggagtaa atatttgatt
     5701 atccaaataa aaataaattt taaaaattaa caaatgagtt gaattttttc cgcatcctcc
     5761 gctaaaacag ttaattaa
//
LOCUS       DAZOZY010000231         5736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZOZY010000231 DAZOZY010000000
VERSION     DAZOZY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.234"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..128)
                     /locus_tag="ACHURQ_003781"
     CDS             complement(<1..128)
                     /locus_tag="ACHURQ_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0825451.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATE"
     gene            complement(186..419)
                     /locus_tag="ACHURQ_003782"
     CDS             complement(186..419)
                     /locus_tag="ACHURQ_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR0825452.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            808..1353
                     /locus_tag="ACHURQ_003783"
     CDS             808..1353
                     /locus_tag="ACHURQ_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR0825453.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1328..3253
                     /locus_tag="ACHURQ_003784"
     CDS             1328..3253
                     /locus_tag="ACHURQ_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0825454.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3250..3456
                     /locus_tag="ACHURQ_003785"
     CDS             3250..3456
                     /locus_tag="ACHURQ_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR0825455.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3453..5054
                     /locus_tag="ACHURQ_003786"
     CDS             3453..5054
                     /locus_tag="ACHURQ_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR0825456.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5035..>5736
                     /locus_tag="ACHURQ_003787"
     CDS             5035..>5736
                     /locus_tag="ACHURQ_003787"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0825457.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRET"
BASE COUNT         1324 a         1416 c         1707 g         1289 t
ORIGIN      
        1 ttctctgtgg ctaaatagta aatgtaatgt gagacattgt gacgttttag ttcagaataa
       61 aaccagtgat agtttaaatt atttcgcact ttatcgaata tttgtttaaa aatggcaacc
      121 tgagccattg tagtaccttc catgtgatat gagggggcgt agtctgcacg attatctaaa
      181 ttgcttcaat ctggtctaac ctgttttctg agcaattcag taatgtcact cttttctttg
      241 tttgcttcag gcgaaactct tttttctgag cacagtctcc ggcggcaggc ttcaatgacc
      301 caggctgaga aattcccgga ccctttttga acaagagcga tgttaatttg ttcaatcatt
      361 tggttaggaa agcggatgtt gcgggttgtt gttctgcggg ttctgttctt cgttgacatg
      421 aggttgccct gtattcagtg tcgctgattt gtattgtctg aagttgtttt tacgttaagt
      481 tgatgcagat caattaatac gatacctgcg tcataattga ttatttgacg tggtttgatg
      541 gcgtagatgc acgttgtgac atgtagatga taattattat cattttgcgg gtcctttccg
      601 gcgatccgac aggttacggg gcggaaggcg cgcgggtttt cgctatttat gacaattttc
      661 cggtttaagg cgtttccgtt cttcttcgcc gtaacctaat gtttttattt aaaacacccc
      721 ctgaaaagaa aggaaacgac aggtgctgaa aacgggcttt ttggcctctg tcgtttcctt
      781 tctctgtttt tgtccgtgga atgaacaatg gaagtcaaca aaaagcagct ggctgacatt
      841 ttcggtgcga gtatccgtac cattcagaac tggcaggaac agggaatgcc cgttctgcga
      901 ggcggtggca agggtaatga ggtgctttat gactctgccg ccgttataag atggtatgcc
      961 gaaagggatg ctgaaattga gaacgaaaag ctgcgccggg aagttgaaga actgcggcag
     1021 gccagcgaga cagatctcca gccagggact attgagtacg aacgccatcg acttacgcgt
     1081 gcgcaggccg acgcacagga gctgaaaaat gccagagact ccgctgaagt ggtggaaacc
     1141 gcattctgta ctttcgtgct gtcgcggatc gcaggtgaaa ttgccagtat tctcgacggg
     1201 atccccctgt cggtgcagcg gcgttttccg gaactggaaa accgacatgt tgatttcctg
     1261 aaacgggata tcatcaaagc catgaacaaa gcagccgcgc tggatgaact gataccgggg
     1321 ttgctgagtg aatatatcga acagtcaggt taacaggctg cggcattttg tccgcgccgg
     1381 gcttcgctca ctgttcaggc cggagccaca gaccgccgtt gaatgggcgg atgctaatta
     1441 ctatctcccg aaagaatccg cataccagga agggcgctgg gaaacactgc cctttcagcg
     1501 ggccatcatg aatgcgatgg gcagcgacta catccgtgag gtgaatgtgg tgaagtctgc
     1561 ccgtgtcggt tattccaaaa tgctgctggg tgtttatgcc tactttatag agcataagca
     1621 gcgcaacaca cttatctggt tgccgacgga tggtgatgcc gagaacttta tgaaaaccca
     1681 cgttgagccg accatccgcg atattccgtc gctgctggcg ctggctccgt ggtatggcaa
     1741 aaagcaccgg gataacacgc tcactatgaa gcgtttttcc aatggtcgtg gcttctggtg
     1801 cctgggcggt aaagcggcaa aaaactaccg tgaaaagtcg gtggatgtgg cgggttatga
     1861 tgaacttgct gcctttgatg aggatattga acaggaaggc tctccgacgt tccttggcga
     1921 caaacgtatt gaaggctcgg tctggccaaa gtccatccgt ggctccacgc ccaaagtgag
     1981 aggcacctgc cagattgagc gtgcagccag tgaatccccg cattttatgc gttttcatgt
     2041 tgcctgcccg cactgcgggg aggagcagta tcttaaattt ggcgacaaag agacgccgtt
     2101 tggcctcaaa tggacgccgg atgacccctc cagcgtgttt tatctctgcg agcataatgc
     2161 ctgcgtcatc cgccagcagg agctggactt tactgatgcc cgttatatct gcgaaaagac
     2221 cgggatctgg acccgtgatg gcattctctg gttttcgtca tccggtgaag agattgagcc
     2281 acctgacagt gtgacctttc acatctggac agcgtacagc ccgttcacca cctgggtgca
     2341 gattgtcaaa gactggatga aaacgaaagg ggatacggga aaacgtaaaa ccttcgtaaa
     2401 caccacgctc ggtgagacgt gggaggcgaa aattggcgaa cgtccggatg ctgaagtgat
     2461 ggcagagcgg aaagagcatt attcagcgcc cgttcctgac cgtgtggctt acctgaccgc
     2521 cggtatcgac tcccagctgg atcgctacga aatgcgcgta tggggatggg ggccgggtga
     2581 ggaaagctgg ctgattgatc ggcagattat tatgggccgc cacgacgatg aacagacgct
     2641 gctgcgtgtg gatgaggcca tcaataaaac ctatacccgc cggaatggtg cagaaatgtc
     2701 gatatcccgt atctgctggg atattggcgg gattgacccg accattgtgt atgaacgctc
     2761 gaaaaagcat gggctgttcc gggtgatccc cattaaaggg gcatccgtct acggtaagcc
     2821 ggtggccagc atgcctcgta agcgaaacaa aaacggggtt taccttaccg aaatcggtac
     2881 ggataccgcg aaagagcaga tttataaccg cttcacactg acgccggaag gggatgaacc
     2941 gcttcccggt gccgttcact tcccgaataa cccggatatt tttgatctga ccgaagcgca
     3001 gcagctgact gctgaagagc aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg
     3061 ggacagcaaa aagcgacgca atgaggcgct cgactgcttc gtttatgcgc tggcggcgct
     3121 gcgcatcagt atttcccgct ggcagctgga tctcagtgca ctgctggcga gcctgcagga
     3181 agaggatggt gcagcaacca acaagaaaac actggcagat tacgcccgtg ccttatccgg
     3241 agaggatgaa tgacgcgaca ggaagaactt gccgctgccc gtgcggcact gcatgacctg
     3301 atgacaggta aacgggtggc aacggtacag aaagacggac ggcgagtgga gtttacggcc
     3361 acttccgtgt ctgacctgaa aaaatacatt gcggagctgg aggtgcagac cggcatgaca
     3421 cagcgacgca ggggacctgc aggattttat gtatgaaaac gcccaccatt cccacccttc
     3481 tgggaccgga cggcatgaca tcgctgcgtg aatatgccgg ttatcacggc ggtggcagcg
     3541 gatttggtgg gcagttgcgg gcgtggaacc caccgggtga aagtgtggat gcagccctgc
     3601 tgcccaactt tacccgtggc aatgcccgcg cagacgatct ggtacgcaat aacggctatg
     3661 ctgccaacgc catccagctg catcaggatc atatcgtcgg gtcttttttc cggctcagtc
     3721 atcgcccaag ctggcgctat ctgggcatcg gggaggaaga cgcccgtgcc ttttcccgcg
     3781 aggttgaagc ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc
     3841 gaaaacgcac gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg
     3901 aactgttcgt tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc
     3961 ggatggtcag cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc
     4021 gtgccggtgt gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg
     4081 gctatcctgg ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc
     4141 gcgcctcgtt cattcacgtt tttgaacccg tggaggacgg gcagacccgc ggtgcaaatg
     4201 tgttttacag cgtaatggag cagatgaaga tgctcgacac gctgcagaac acgcagctgc
     4261 agagcgccat tgtgaaggcg atgtatgccg ccaccattga aagtgagctg gatacgcagt
     4321 cagcgatgga ttttattctg ggcgcgaaca gtaaggagca gcgggaaagg ctgacgggct
     4381 ggattggtga aattgccgcg tattacgccg cagcaccggt ccgtctggga ggcgcaaaag
     4441 tgccgcacct gatgccgggg gactcactga acctgcagac ggctcaggac acggataacg
     4501 gctactccgt gtttgagcag tcactgttgc ggtatatcgc tgccgggctg ggtgtctcgt
     4561 atgagcaact ttcccggaat tacgcccaga tgagctactc cacggcacgg gccagtgcga
     4621 acgagtcgtg ggcgtacttt atggggcggc gaaaattcgt cgcatcccgt caggcgagcc
     4681 agatgtttct gtgctggctg gaagaggcca tcgttcgccg cgtggtgacg ttaccttcaa
     4741 aagcgcgctt cagctttcag gaagcccgca gtgcctgggg gaactgcgac tggataggct
     4801 ccggtcgtat ggccatcgat ggtctgaaag aagttcagga agcggtgatg ctgatagaag
     4861 ccggactgag cacctacgag aaagagtgcg cgaaacgcgg tgacgactat caggaaattt
     4921 ttgcccagca ggtccgtgaa acgatggagc gccgcgcagc tggtcttaaa ccgcccgcct
     4981 gggcggctgc ggcatttgaa tccgggctgc gacaatcaac agaggaggag aagagtgaca
     5041 gcagagctgc gtaatctccc gcatattgcc agcatggctt ttaatgagcc gctgatgctt
     5101 gaacccgcct atgcgcgggt tttcttttgt gcgcttgcag gccagcttgg gatcagctgc
     5161 ctgacggatg cagtatccgg cgacagcctg actgccggag aggcacccgc ggcgctggcg
     5221 ttatccggtg atgatgacgg accacgacag gcccgcagtt atcaggtaat gaacggcatc
     5281 gccgtgctgc cggtgtccgg cacgctggtc agccggacgc gggcgctgca gccgtactcg
     5341 gggatgaccg gttacaacgg cattatcgcc cgtctgcaac aggctgccag cgacccgatg
     5401 gtggacggca ttctgctcga tatggacaca ccgggcggaa tggtggcggg agcatttgac
     5461 tgcgctgaca tcatcgcccg tgtgcgtgac ataaagccgg tatgggcgct ggccaatgac
     5521 atgaactgca gcgcaggtca gctgcttgcc agtgccgcct cccggcgtct ggtcacgcag
     5581 accgcccgga caggctccat cggcgtcatg atggctcaca gtaattacgg cgctgcactg
     5641 gagaaacagg gcgtggaaat cacgctgatt tacagcggca gccataaggt ggatggcaac
     5701 ccctacagcc atctaccgga tgatgtccgg gaaaca
//
LOCUS       DAZOZY010000232         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZOZY010000232 DAZOZY010000000
VERSION     DAZOZY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.235"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHURQ_003788"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHURQ_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR0825458.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHURQ_003789"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHURQ_003789"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR0825459.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHURQ_003790"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHURQ_003790"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR0825460.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHURQ_003791"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHURQ_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR0825461.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZOZY010000233         5633 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZOZY010000233 DAZOZY010000000
VERSION     DAZOZY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.236"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(78..197)
                     /locus_tag="ACHURQ_003792"
                     /pseudo
     CDS             complement(78..197)
                     /locus_tag="ACHURQ_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
     gene            complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHURQ_003793"
     CDS             complement(302..1804)
                     /gene="dtpA"
                     /locus_tag="ACHURQ_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR0825462.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2416..3051)
                     /gene="nth"
                     /locus_tag="ACHURQ_003794"
     CDS             complement(2416..3051)
                     /gene="nth"
                     /locus_tag="ACHURQ_003794"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR0825463.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="ACHURQ_003795"
     CDS             complement(3051..3746)
                     /gene="rsxE"
                     /locus_tag="ACHURQ_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR0825464.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="ACHURQ_003796"
     CDS             complement(3750..4370)
                     /gene="rsxG"
                     /locus_tag="ACHURQ_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR0825465.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="ACHURQ_003797"
     CDS             complement(4374..5432)
                     /gene="rsxD"
                     /locus_tag="ACHURQ_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR0825466.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5433..>5633)
                     /locus_tag="ACHURQ_003798"
     CDS             complement(5433..>5633)
                     /locus_tag="ACHURQ_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825467.1"
                     /translation="EPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAA
                     VAAAIARAQAKKAAQQKVVNED"
BASE COUNT         1473 a         1432 c         1389 g         1339 t
ORIGIN      
        1 ccggaaacaa gtgagcgttt ccggattctt acacagccac tggatcggtc aactgatcct
       61 taactgatcg gcattacacc gttttcgaga cgttttttca ttaaatccac actgacgagg
      121 gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca ggcacccggt
      181 ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg gtagtgcgtt
      241 gctgaacaaa aaaaagccgc taacgaaaag ttagcggctt gtaaaagagt ttccctgaat
      301 tttacgctac agcagctttc gccgctttgt ctgcagcgtc atcctgcgtc atacggtgca
      361 gtttaggcgc ggtcagcagc atcagtactg caataatggc agtagcgaca ccaatctgca
      421 agaatacgcg accataaact tccagtgaca tcagcgggtc ggtaacgtta tccggcacag
      481 ccatcatacc cgcaacataa ccaccaatca ggtttgcacc ggcagtggtc aggaaccagc
      541 tacccataat gaagcccatc agacgctgcg gaacgagttg tgcaaccatt gccagaccca
      601 gaccagagat catcagttcc ccgatgctct gcaggccata gcttgcgacc agccagctta
      661 cagacacgat accagcgtca gacgcgaatt tcgcacccag cggcagaatc aggaacgcac
      721 cagaacacat caccatgccg attgcaaact tggttggcat cggcagggta tcgcccatct
      781 tgttatagat agcggccaga atcggactac cgatgatgat ccagaacggg ttcagtgcct
      841 gatactgctc aggttctacg gccagaccca gaatggagtg ctcaacgtta cgaatcgcaa
      901 agaagttcag tgacgttggc atctggctgt acagcacgaa gaagataatg gcttcgagca
      961 tcaggatgaa ggcaacgatc attttacgac gcgcagcacc tttcatcgcg aaggcttctt
     1021 taccgaagat aaccacgata ccgaaggcaa caacgcccag cgccatacgc gcaacttcct
     1081 gattgtgcag cagccaggtg gcgatagcga tcagtgccac aacaccaata atggtcagca
     1141 gcaggttacg gtagttgata ggctcgaagt ctggttttga accgtactgt ttaacccagc
     1201 gttggcagaa ggcgaagtta acgatagtga tcagcaggcc tacaacgctc aacgcaaacg
     1261 caacactcca gccgtatttc gcggccagcc acggcgtagc aatcatagag aagaaagagc
     1321 cgatgttgac ggacatgtag tacatggtga atgcaccgtc cagacgcggg tcgtttttct
     1381 catagcatgt agaaagcaga gaagatgggt tagctttaaa caggccgtta ccgaccgcaa
     1441 tagccgccat acccatataa acgataccgg cgtcgtgacc agaccaggca accagagcat
     1501 aaccaatcgc cagcacaata gcgccgagca taattacgcg tttagtaccc agtaccttgt
     1561 cacctaacca gccgccgata gcgaccagac cataaaccag ggcactaaag gaagagaaaa
     1621 gggtgattga atccgcttca gacataccca gttgtttaac caggtaaaca gccataattc
     1681 cttgtaggcc gtaataacca aaacgttccc ataactcaat cgagaagatg agatagaacg
     1741 ccttcggttg tttgaaagcg ttcaaactga cgctttcagt tggtttttgg tttgcagtgg
     1801 acacttttac ctcttttttt acatcccata ttaacggggg ggctttctcg cgttatgtct
     1861 aaaaaacata cgcgcaattg ttatagtggg aggggaaacg gcaggtaatg ttcactatct
     1921 tgcactttca gacaagtcct ttgtaataat ctgttacata taactgaggc ggggaattca
     1981 accattccgg gaaatgttat ccagcgttaa atttttcaaa aaacgtaatg gcagattttt
     2041 tcactgcact gagagggggg gtgatggctt aacggcgata tgttctgcta tttggcgttt
     2101 ttactagtga gatagtccag tttctgaaaa atagccagtg taatgttttg taggtcaata
     2161 tttgttgcgt gtgtaaattt aaattatttt aacctgattc ttgcaatgtt tttacattgc
     2221 gacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa aaaacaatta
     2281 acctgcattt ttggttatcc gcaaacggat tacctgatga taacgctaag caatggcatc
     2341 attcacgtta aaaagatgtt ttaaaagagg ataaagaaag gttatcaatg gggtaatcgg
     2401 tgttacccct tttcttcaga tgtcaacttt ctctttgtat tcacaaagat cttcaataat
     2461 acaagagcca cagcggggct tgcgggcaat gcaggtataa cgcccgtgca ggatcaacca
     2521 atggtggcag tcgactttaa actctgccgg aaccactttc agtagctttt cttctacctg
     2581 ttcgacgttt ttccccggcg caaattgagt acgattacaa acgcggaaaa tgtgcgtgtc
     2641 gacagcaata gtcggccagc cgaatgcagt gtttaatacg acgttggctg ttttacgacc
     2701 tacgccgggc agggcttcaa gcgcagcacg atcttccgga acctcgccat tatgctgctc
     2761 cagtaagata cggcaggttt tgatgatatt ttctgctttg ctgttataaa gcccaatcgt
     2821 tttgatatag gttttaaccc cttcaacgcc cagttcaagc atcgctgcag gcgtattcgc
     2881 caccgggtaa agtttcgccg tcgccttatt aacactgaca tcggtcgcct gagcggaaag
     2941 cagtacggca atcagcaatt caaaaggcga actgaaatta agctcggtgg tgggatgagg
     3001 attgttctca cgcaggcgag tgaggatctc caggcgtttt gctttgttca tcagacattc
     3061 cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt cagtacggcg ctttttcatt
     3121 ctttcatcaa tcaggtattt tcctgccagc atcagtccca ggccaataaa tgcacctggt
     3181 ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa tctccacgcg taatactttt
     3241 gcccagctac ctaacagcgc atctgcaccg tcaaacaatg tgccattgcc gataatttcg
     3301 cgtagtgaac ccagcacgaa catggcgcag gttgcgccca taccaattga aaagccgtcc
     3361 agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt cagcgcggcc cacaacgata
     3421 cagttagtga caatcagcgg aataaaaatc cctaatgatt gatacaggcc aaaggcgtag
     3481 gcgttgatca gcatctgtac agcgctgacc accgaggcga tgatcatcac gtaaatggga
     3541 atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa tggtcaggtt ggtcagcgtc
     3601 agtaccagcg tagtcgcaag tcctaaaccc agagcgttag tggcagtgga cgtgaccgcc
     3661 aacagaggac aaaggccgag caactggacc agcgcagagt tgtttttcca caacccctga
     3721 acaataacgt ctttaatttc gctcacgctt tattctccac aggcaggaag ttgagaaagt
     3781 tgtgccggta acgtctgagc gtacaatccg gcgcgtttta ccgcattaac caccgcgcgg
     3841 ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac catctttctt caccgcccag
     3901 tgcgcatcat ccgcaccact gattttttta cccgcaaaat gggtgatcca gtcagaaagg
     3961 cgcagttcga ttttatcgcc aagccctggc gtttcgtggt gctctgtcac gcgcgtgcca
     4021 agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa tcgcaccgga atagccatct
     4081 ggcgcggttg cttccagaac ggcggctacc ggtttgtcat cctgtttggc gatgtaaacc
     4141 cgatgctcac ctttacctaa ctctggcgca gttaccagat agcaactctg tgccagcgta
     4201 ttgttatagc gttcggctgg cagcacctga tcaaataatg ccttttgttg cagactggcc
     4261 tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg cagttaaccc tgttgaaccc
     4321 gctgcaaata gcgctaacgt aatgccgtgt tttcggatag ttttcagcat ggtttaccct
     4381 ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga tcagaggaac cgtgatgttc
     4441 gccagcagga cggcaaaagc cacgccgtca ggatagccgc cgaaactgcg gatcaaccag
     4501 actaataagc ccgcaagcgc gccgaaaata agacgaccac gattggtcgt agaagcggta
     4561 accgggtcag tcaaaataaa gaatgcgccg agcatggtcg caccagacag cagatgaatt
     4621 tgcggtgctg ccagtgtttc tggtgagaac aaccagccca acgttgcgca taacgccagc
     4681 gttactaaga agctgagggg aatatgccag cgaatcgctt tctgccatag caaccacagg
     4741 ccgccagcca gccaggcgag atttacccat tgccaaccag cgcccgccag aataccgctg
     4801 tagatcggat attgcataat ctgttcaacc gaatgaccgg cacggacaga ggttttaaat
     4861 gtatccagcg gtgtcgcctg actaatgcca tcaataccta agcgtagtgt gttcatatca
     4921 ccaccactgg cggtatgccc gctgaaaata acctgtatgg cgtcgataaa accagggatg
     4981 ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct gcaccgggaa ggagatcagt
     5041 aagaccacat aaccaatcat tgccggatta aacgggtttt gccccagacc gccatacaac
     5101 tgtttagcga tgatcaccgc aaacaccgta cccagcacga ccatccacca tggcgcgagg
     5161 gggggaatac ttaccgccag caataagcct gtcagcaatg ctgagttatc tttcaacgtt
     5221 gcggctaccg actgcttgcg tagtttgagt acgagagctt cggctaacag agtactaacc
     5281 gatgccaaca ggatctgaac gagagtaccc caaccaaaaa accacagttg cgctgcgatt
     5341 cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg atgtctggcg ctggttatgg
     5401 gtataagggg agctagctat tctgaatacc atttagtcct cgtttacaac cttctgctgg
     5461 gcggcttttt tggcctgagc gcgggcaata gccgcggcaa ctgccgcttt gcgcggatca
     5521 acctgttctt ctggttctgc gttagtctgt tgctgttcca gcttgcgcgc tttggcacgg
     5581 gcaatagcgg cttcgacggc ggctttgcgc ggatcaacct gttcttctgg ttc
//
LOCUS       DAZOZY010000234         5620 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZOZY010000234 DAZOZY010000000
VERSION     DAZOZY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5620
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.237"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..513)
                     /locus_tag="ACHURQ_003799"
     CDS             complement(<1..513)
                     /locus_tag="ACHURQ_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR0825468.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="ACHURQ_003800"
     CDS             complement(759..1211)
                     /locus_tag="ACHURQ_003800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0825469.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHURQ_003801"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="ACHURQ_003801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR0825470.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHURQ_003802"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="ACHURQ_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR0825471.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="ACHURQ_003803"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="ACHURQ_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHURQ_003804"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="ACHURQ_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR0825472.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5620)
                     /locus_tag="ACHURQ_003805"
     CDS             complement(5494..>5620)
                     /locus_tag="ACHURQ_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0825473.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1468 c         1460 g         1353 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaatattaat agcgatgtgc
//
LOCUS       DAZOZY010000235         5613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZOZY010000235 DAZOZY010000000
VERSION     DAZOZY010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.238"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHURQ_003806"
     CDS             complement(104..418)
                     /locus_tag="ACHURQ_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR0825474.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHURQ_003807"
     CDS             complement(423..638)
                     /locus_tag="ACHURQ_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR0825475.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHURQ_003808"
     CDS             complement(789..2642)
                     /locus_tag="ACHURQ_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR0825476.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHURQ_003809"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHURQ_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHURQ_003810"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHURQ_003810"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHURQ_003811"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHURQ_003811"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHURQ_003812"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHURQ_003812"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHURQ_003813"
     CDS             complement(3908..4966)
                     /locus_tag="ACHURQ_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR0825477.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHURQ_003814"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHURQ_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..>5613)
                     /locus_tag="ACHURQ_003815"
     CDS             complement(5539..>5613)
                     /locus_tag="ACHURQ_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0825478.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1338 a         1583 c         1321 g         1371 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZOZY010000236         5600 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZOZY010000236 DAZOZY010000000
VERSION     DAZOZY010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.239"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(483..4025)
                     /locus_tag="ACHURQ_003816"
                     /pseudo
     CDS             complement(483..4025)
                     /locus_tag="ACHURQ_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4093..>5600)
                     /gene="tssI"
                     /locus_tag="ACHURQ_003817"
     CDS             complement(4093..>5600)
                     /gene="tssI"
                     /locus_tag="ACHURQ_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0825479.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGVNQIETVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
BASE COUNT          991 a         1834 c         1465 g         1310 t
ORIGIN      
        1 aaacataaaa atatcattaa aaatacacat tacaacccca gcgtcccaag ctcttcaaaa
       61 aacttcattg gagaatcttt gatattctca atcttttcac cataaacgtc taatattgat
      121 ggttcaccag ggtcatcaaa ctgctcacca taaggtaaaa tacccaataa atttctccaa
      181 cacctatctc taccattttc ctgaagtgat tctgataatt gtaaatttgc tctgttatat
      241 gcagctgtcg cagattttat attccacgcc cctaaagcca tagaagctgc accgactggg
      301 gcagcaatga tcgttccatc ggcagcaaca cttataccga attttgtcat gccacttgca
      361 tacagccttg ctgaattagc agtatttaaa ctagctgtaa acatttttgt cgggttaaac
      421 ggaaggttat ccagtttatt atttatatca tctataaaat gactacaact ctcacatgag
      481 aataacccca acggatctat cctgctcacc ggattcagcg gatactgata cagattccac
      541 ccacctttca gcccaatggg gtcctgggtg atgtaccgtc tctgcaacgg atcatagtac
      601 cggtgacgat tgtagtacag ccccgactcc tcatcatact gctgccccgg cagacggtat
      661 ggctgataca catgatgcgg gttctcctca tttagctggt tgccccactc atcatacgcc
      721 ccgctccaca ccgtatcgcc gtcttcgctg atgagcgcca gcggctggcc ccgatggtcg
      781 cagtggtaaa gatgaacgtt tcgcactggt gtgtattccg gctccacctg tctggccagt
      841 tgctccaccg tcagcccgca ctgcgcaagc cacacccggc tttcactgct cacgcggtct
      901 gcccggattt cttcctccag tctgtccagc agccgcacca gttcagccgg aaataccacg
      961 ccgtgcccgt cctcgctccc ttcctgctgc agcttctccg ccaggctgcg gcgctgcgct
     1021 ttctcccgct cgccgttctc tgtctcgacc cggatgagcg gcgtgaagct tcccggctcg
     1081 tataccgtct ggatacgtgt ggtgtcggtc tgtaccgtcg tcagcctgtc gccgtcccag
     1141 ccgtaccatg tctcctccgg tttacgcgac agcgacatcc accccgtcag gtcacgctcc
     1201 cgccgccata cccgtttcgc cattcgccgt cccagcgggt cgtagaggta gcggctctcg
     1261 accagtggct cgccatgctg tatccgcgtg tggaacacca ggcggtgctg gctgtcgtag
     1321 tggtgggtcc gctcgtcgtc cgtccgtatc acacccgtcg ggatgcggtc cgtcttctcc
     1381 gtcagtctgc cgtattcatc atagtggtaa acatagtgcg catcctctgc gatgcggtta
     1441 tccggccacg ccgtgagcgt gctgtccggg tgtagctccg ggtccggcag gcggtttccc
     1501 gccgggtcgg tggcatacgg gatgcggatg tccaggtccg gtgcgagggt gcgcaccgtc
     1561 tcacggtgca gcctgtcccg cgtatactcg accagcggcg tcccgcccag cttcatgccc
     1621 gccaggtaac cgctgccgta ggtcagccac tccaccggcg gcaggctgtc cggcgtgacg
     1681 cggtttgcca gcccctgttc gttgtaggcg tgtttcgtct catgctgcca cagcagttcc
     1741 cccgtctccg ggttctccac cgtctgccgt tcgcccgtca ggcggccctt atcgtcgtag
     1801 ccgtagtgga cagcgacacg gtggccttcg ctcaggtggc tgatttctct cagccagccg
     1861 tggccgtcat actgccactg ctctgccggg tcgccgttca ccgtccggtg cgtgatgcgg
     1921 tccgacgcat cgtagtgcca gagggtgaca agcccctcat cctcactctg cgtgagtttt
     1981 ccggtcaggt cataatgata acgttgcgtc cgcccgtcaa agccgccctg ctgtaccagc
     2041 cggtccagcg catcgtagct gaagtcgctg tgactgccgt tctcgttagt cagactgatg
     2101 acacgtccgg cagcatcgta ctccatactg cgcgtcagcc cgccctgcgt ggtgctgacc
     2161 gcctttcccc acgcatcgta ctgtgtctcg ctccggttgc cgtccggggt gataacggca
     2221 gtcaggtcgc ctgcggcgtt gtactcatac tgcgtttcac ggccctgtgc gtctttcacc
     2281 gaggttaacc ggccacggtt gtcatagtgg cggtaaaggc tgataccttc ctcgcggtga
     2341 accgccgtca tctggccgaa gcggtcgtat tcatagcggg tctggtagcc cgagcagtca
     2401 gtgaacgcca gcaactgccc gtagcggctc cacgtcatct gccgggtgct gcccgtcgca
     2461 tccgttgtcg tcgccggtaa ctcactgtgc gcgtcatcgt agcggtagcg tactgtctcc
     2521 ccgctgcgcg atgtctccga taccagcctg cccggttcat catattcccg gcggctctcc
     2581 agcccgtccg ggtacaccac cgccgtcagc tggttcccgt cgttatagta aaatttcgtc
     2641 tcccgcccgt ccggtgtggt gatgtccgtg atatcgccgg acaccacatt cagaccgtac
     2701 tctgtccgcc gtcccgccgc gtccgtctgc gccgtgagcc ttcctgccgc gtcatacccg
     2761 ctgcgcgtga cgctgccgtc cgccagttct tttttcacca cccgtttcag cccggccccg
     2821 ccttctgtat gcagcacctc acgccggttc aggctgtcgg tgacggtgat gcggtcctgc
     2881 tcataaagat agcggtagct taaccctgcc gggttcagtt gctccaccac ccgccccgta
     2941 tcgtcgtagc ggtagcgcat ctccggcctt cccgcgtaac ggtgcgccac catccggccc
     3001 gggtgctgcg cgtcatacgt gaaagcgcgc acctgcgtat tgctgcggtc atataccgcc
     3061 agcagttcac cggcttccgt atacgtgtac cgcaccagtg gcgcagcggg caggctctcc
     3121 gggtatgccg ggtcgtgcat caaccacacc gccgaaaggc ggatacccct gtcggggccg
     3181 tattcggtac caggcagtgt gtcggggaac gctgaggctg agagagggcg ggaactgtca
     3241 gaagaagata gcgaagaggt gcgggcctct tccgcacgct gcgcctgcgt ggtcagcacc
     3301 agacggaact cccgcccggc accgtccgtc acgtcggtga tttccccggc caggtcaccg
     3361 gcggcctcac gccggtacgt cagcgtccgc catcccggta agcacccggt acggcggcag
     3421 cggcgctggc agtacgtcct cagcacccgg cacccgctca gcccccccca gtatccacca
     3481 cggcccctgt gcgctgttgg tcgccaggta aagatgcggg cttaaccgga tatccggcgc
     3541 agcgcccccc acagccgcgc cagcgtatgg ccgtccggct gtgctgcctt accaccgcgc
     3601 accagccaca ttgactcact gcggctgtac accgcctccc ccggcagcag cggctcaaag
     3661 tgaatgctcc gcccgccgtt gtcgttgagt atcagtccgt catcacgtag ctgtaagcgg
     3721 atatcagaag gcgctttcca gccggggccg aaaacgccca ccggtgcagg cgtcttcgtc
     3781 cggtagctgc tgtaggtgcg ggagagaatg aacggcagcg ggccgggcag cgcaaggtcc
     3841 gtctcgccgg gcagcacctt cgcccccagc agcggattta ccgggttgcc cgaagtcatc
     3901 ccgcccggac acaccgagca cgccacgccg gtgggcgcgc caattcttac acctgccgaa
     3961 ccctggacaa tgggaccgcc atactgagtc atatctccct gacgcgccgc tggttttccg
     4021 ctcatgattc aaattccttc ttctgtttat ttttaatgct ggtttgttac tgaataccga
     4081 acaggtacgg gatcagtact ctctcatatc cggcgtatcc tgcttttcat caggggtctt
     4141 cggtggggat ttcgaggctg cacagttcca gttaatcaac agtgagtcac cattaacctg
     4201 ctccttaccc tgcaaatgaa tttttgtgcc gttgagaaaa atttgtccgt ccttcgtcag
     4261 caccagcctt gccttaccac agcagagctc cagatgctca ccggcggagt aaatcgcggt
     4321 ctgcccggta tcatcctttt tcgttttacc aacggtgaag gtaacgttat ttccgacttt
     4381 gacgtcataa cccagcccga ccctcaacga tttatgcaaa ccgatctgcg aggactgcat
     4441 cagtgccacc gaggtgttca taatgccacc taccgtcgtc tgatacgcca cgcccaccgt
     4501 cagcgcacga acaagtccaa tcgtttccac ctgaacagaa cccactttaa ttatctggtt
     4561 actgcccacc gtctctatct ggttaacgcc caccgtctct atctggttaa cgcccaccgt
     4621 ctctatctga ttgttggtaa ccgcaatcat ctgattgttg ccaatggttt cagcatggtt
     4681 gtttatcaca tccgtggtgc ggttattcag cacctcggtg ttcatgttct tctgcgcgtg
     4741 gatgtagacc tgttctttcc ctgtcgcatc gtcaaacttc agttcattaa acccgctgcc
     4801 cttataggtt ttcgaacgaa tggtcatctg cgtcttcgtc cccggcaggc tgccgggtgt
     4861 gcggttttcc tggtggtagg tacgccccat aataatcggc tggtccgggt cgccgttgag
     4921 gaagtccaca atcacctcct gacccacacg cggtatcgcc aggttgccaa agccggtgcc
     4981 tgcccacgcc tgtgccacac ggatccagca tgaactgtcc tggtttgacg ggttatagcg
     5041 gtcccagtta aatttcaccc gcacgcggcc atgttcatca cagaagattt cctcgcctgc
     5101 cggtcccgtc acgacggcgc tctgcgggcc atccaccagc ggtttcatca acggctgtgg
     5161 ccgccatgtt ctgtctgccg ggattaccgc aaaatggttc tccagcgtgg tgcctgaacc
     5221 cctgcgtccc ggtaccgcct gcggctgttc gccgtgaaga tcgctcgcca ccacctgcca
     5281 ttcccggttc aggttcgcct gcggatgccc cgtcagcaca attcgccgtc ccggccatat
     5341 ctccggcgaa cggcttgttc cgcgcgccac ttctgcgttg tttcgccagc cgtccatctg
     5401 ccagcgggca aagttctgcc cgtgggcacc cttgaaacgt ccggggtagt catacacttc
     5461 atactgcgtg cgctggtaat cctggtactg gccttcctga tcaaaacgcc ctggccagcc
     5521 gggacgttta aaggtgtagt ctttggtcac tacggaagaa ggacggattt gtgcgctgta
     5581 gcgaaactgg ctgatgcaga
//
LOCUS       DAZOZY010000237         5571 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZOZY010000237 DAZOZY010000000
VERSION     DAZOZY010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.240"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..297
                     /locus_tag="ACHURQ_003818"
     CDS             <1..297
                     /locus_tag="ACHURQ_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0825480.1"
                     /translation="AQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPG
                     MTVETARRILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            307..639
                     /locus_tag="ACHURQ_003819"
     CDS             307..639
                     /locus_tag="ACHURQ_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0825481.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            695..1720
                     /locus_tag="ACHURQ_003820"
     CDS             695..1720
                     /locus_tag="ACHURQ_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0825482.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            1762..2160
                     /locus_tag="ACHURQ_003821"
     CDS             1762..2160
                     /locus_tag="ACHURQ_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0825483.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            2172..2525
                     /locus_tag="ACHURQ_003822"
     CDS             2172..2525
                     /locus_tag="ACHURQ_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0825484.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            2537..3115
                     /locus_tag="ACHURQ_003823"
     CDS             2537..3115
                     /locus_tag="ACHURQ_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0825485.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            3112..3507
                     /locus_tag="ACHURQ_003824"
     CDS             3112..3507
                     /locus_tag="ACHURQ_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0825486.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            3515..4255
                     /locus_tag="ACHURQ_003825"
     CDS             3515..4255
                     /locus_tag="ACHURQ_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0825487.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            4271..4693
                     /locus_tag="ACHURQ_003826"
     CDS             4271..4693
                     /locus_tag="ACHURQ_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0825488.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            4675..5109
                     /locus_tag="ACHURQ_003827"
     CDS             4675..5109
                     /locus_tag="ACHURQ_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0825489.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            5102..>5571
                     /locus_tag="ACHURQ_003828"
     CDS             5102..>5571
                     /locus_tag="ACHURQ_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFR0825490.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSG"
BASE COUNT         1241 a         1438 c         1728 g         1164 t
ORIGIN      
        1 gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg cggcagaaaa cagtcgcatt
       61 atggggatcc tcaactgtga ggaggctcac ggacgcgaag aacaggcacg cgtgctggcc
      121 gaaacccccg gtatgaccgt ggaaacggcc cgccgtattc tggccgcagc accacagagt
      181 gcacagacgc gcagtgacac tgcgctggat cgtctgatgc agggggcacc ggcaccgctg
      241 gctgcaggta acccggcatc tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg
      301 atgtttatga cgagcaaaga aacctttacc cattaccagc cgctgggcaa tagtgacccg
      361 gctcataccg caaccgcgcc cggcggattg agtgcgaaag cgcctgcaat gaccccgctg
      421 atgctggaca cctccacccg taagctggtt gcgtgggatg gcaccaccga cggtgctgcc
      481 gttggcattc ttgcggttgc tgctgaccag accagcacca cactgacgtt ctacaagtcc
      541 ggcacgttcc gttatgagga tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa
      601 cggaccgtgt ttgccggaac ggcaatcagc atcgtttaac tttacccttc atcactaaag
      661 gccgcctgtg cggctttttt tacgggattt ttttatgtcg atgtacacaa ccgcccagct
      721 gctggcggca aatgagcaga aatttaagtt tgatccgctg tttctgcgtc tctttttccg
      781 tgagagctat cccttcacta cggagaaagt ctatctctca caaattccgg gactggtaaa
      841 catggcgctg tacgtttcgc cgattgtttc cggtgaggtt atccgttccc gtggcggctc
      901 cacctctgaa tttacgccgg gatatgtcaa acccaagcat gaggtgaatc cgcagatgac
      961 cctgcgtcgc ctgccggatg aagatccgca gaatctggcg gacccggctt accgccgccg
     1021 tcgcatcatc atgcagaaca tgcgtgacga agagctggcc attgctcagg tcgaagagat
     1081 gcaggcagtt tctgccgtgc tcaagggcaa atacaccatg accggtgaag ccttcgatcc
     1141 ggttgaggtg gatatgggcc gcagtgcggc gaacaacatc acacagtccg gcgtcacgga
     1201 gtggagcaag cgtgacaagt ccacgtatga cccgaccgac gatatcgaag cctacgcgct
     1261 gaacgccagc ggtgtggtga atatcatcgt gtttgacccg aaaggctggg cgctgttccg
     1321 ttccttcaaa gccgtcaagg agaagctgga tacccgtcgc ggctctcatt ccgagctgga
     1381 gacagcggta aaagacctgg gcaaagcggt gtcctataag ggaatgtatg gcgatgtggc
     1441 catcgtcgtg tattccggac agtacgtgga aaacggcgtc aaaaagaact tcctgccgga
     1501 caacacgatg gtgctgggga acactcaggc tcgcggtctg cgcacctatg gctgcattca
     1561 ggatgcggac gcacagcgcg aaggcattaa cgcctctgcc cgttacccga aaaactgggt
     1621 gaccaccggc gatccggcgc gtgagttcac catgattcag tcagcaccgc tgatgctgct
     1681 ggctgatcct gatgagttcg tgtccgttca actggcgtaa tcatggccct tcggggccat
     1741 tttctctctg tggaggagtt catgacgaaa gatgaactga ttgcccgtct tcaggagctg
     1801 ggtgagcaat tgaaccgcga tgtcagtttg acgggaacga aagaagaact ggcgcttcgt
     1861 gtggcagagc tggaagaaga gcttgatgac acggatgaaa ctgccggtca ggacacccct
     1921 ctcagccggg aaaatgtgct gaccgggcat gaaaatgagg tggtatcagc gcagccggat
     1981 accgtgattc aggatacggc tgaactggtc acggtcgtgg cactggtgac gctgcatact
     2041 gatgcacttc acgccacgcg ggataaacct gtggcatttg tgctgccggg aacggcgttt
     2101 cgtgtctctg ccggtgtggc agccgaaatg acagagcgcg gcctggccag aatgcaataa
     2161 cgggaggcgc tgtggctgat ttcgataacc tgttcgatgc tgccattgcc cgcgccgatg
     2221 aaacgatacg cgggtacatg ggaacgtcag ccaccatgac atccggtgag cagtcaggtg
     2281 cggtgatacg tggtgttttt gatgaccctg aaaatatcag ctatgccgga cagggcgtgc
     2341 gcgttgaagg ctccagcccg tccctgtttg tccggactga tgaggtgcgg cagctgcggc
     2401 gtggagacac gctgaccatc ggtgaggaaa atttctgggt agatcgggtt tcgccggatg
     2461 atggcggaag ttgtcatctc tggcttggac ggggcgtacc gcctgccgtt aaccgtcgcc
     2521 gctgaaaggg ggatgtatgg ccataaaagg tcttgagcag gccgttgaaa acctcagccg
     2581 tatcagcaaa acggcggtgc ccggtgccgc cgcaatggcc attaaccgcg ttgcttcatc
     2641 cgcgatatcg cagtctgcgt cacaggttgc ccgtgagaca aaggtacgcc ggaaactggt
     2701 aaaggaaagg gccaggctga aaagggccac ggtcaaaaat ccgcaggcca gaatcaaagt
     2761 taaccggggg gatttgcccg taatcaagct gggtaatgcg cgggttgtcc tgtcccgccg
     2821 caggcgtcat aaaaaggggc agcgttcatc cctgaaaggt ggcggcagcg tgcttgtggt
     2881 gggtaaccgt cgtattcccg gcgcgtttat tcagcaactg aaaaacggcc ggtggcatgt
     2941 tatgcagcgt gtggccggga aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc
     3001 ggtgccgctg accacggcgt ttaaacagaa tattgaacgg atacggcgtg aacgtcttcc
     3061 gaaagagctg ggctatgcgc tgcagcatca actgagaatg gtaataaagc gatgaaacat
     3121 actgaactcc gtgcagccgt actggatgca ctggagaagc atgacaccgg ggcgacgctt
     3181 tttgatggtc gccccgctgt ttttgatgag gaagattttc cggcaattgc cgtttatctc
     3241 accggcgctg aatacacggg cgaagagctg gacagcgata cctggcaggc ggagctgcat
     3301 attgaagttt tcctgcctgc tcaggtgccg gattcagagc tggattcgtg gatggagtcc
     3361 cggatttatc cggtgatgag cgatatcccg gcactgtcag atttgatcac cagtatggtg
     3421 gccagcggct atgactaccg gcgcgacgat gatgcgggcc tgtggagttc agccgatctg
     3481 acttatgtca ttatctatga aatgtgagga cgctatgcct gtaccaaatc ctacaatgcc
     3541 ggtgaaaggt gccgggacca ccctgtgggt ttataagggg agcggtgacc cttatgcgaa
     3601 cccgctttca gacgttgact ggtcgcgtct ggcaaaagtt aaagacctga cgcccggcga
     3661 actgaccgct gagtcctatg acgacagcta tctcgatgat gaagatgcag actggactgc
     3721 gaccgggcag gggcagaaat ctgccggaga taccagcttc acgctggcgt ggatgcccgg
     3781 agagcagggg cagcaggcgc tgctggcgtg gtttaatgaa ggtgataccc gtgcctataa
     3841 aatccgcttc ccgaacggca cggtcgatgt gttccgtggc tgggtcagca gtatcggtaa
     3901 ggcggtgacg gcgaaggagg tgattacccg cacggtgaag gtcaccaatg tgggacgtcc
     3961 gtcgatggca gaagatcgca gcacggtgac ggcgacgacc ggcatgacgg tgacacccgc
     4021 cagtgcttcc gtagtgaaag ggcagagcac cacgctgacc gtggcattcc agccggatgg
     4081 cgcaaccgac aagagcttcc gtgcggtgtc tgcggataaa acaaaagcca ccgtgtcggt
     4141 cagtggtatg accatcaccg tgaaaggtgt tgctgcaggc aaggtcaaca ttccggtcgt
     4201 atctggtaat ggtgagtttg ctgcggttgc agaaatcaac gtcaccgcca gttaatccgg
     4261 agagtcagcg atgttcctga aaaccgaatc atttgaacat aacggtgtga ccgtcacgct
     4321 ttctgaactg tcagccctgc agcgtattga gcatctcgac ctgatgaaac ggcaggcaga
     4381 acaggcggag tcagacagca accggaagtt tactgtggaa gacgccatca gaaccggcgc
     4441 ttttgtggtt gcgatgtccc tgtggcataa ccatccgaag aagacgcaga tgccgtccat
     4501 gaatgaagcc gttaaacaga ttgagcagga agtgcttacc acctggccca cagaggcaat
     4561 ttctcatgct gaaaacgtgg tgtaccggct gtccggtatg tatgagtttg tggtgaatga
     4621 tgctcctgaa caggcagagg acgccgggcc tgcagagcct gtttctgcgg gaaagtgttc
     4681 gacggtgagc tgagttttgc cctgaaactg gcgcgtgaga tggggcgacc cgactggcgc
     4741 gccatgcttg ccgggatgtc atccacggag tatgccgact ggcaccgctt ttacagtacc
     4801 cattattttc atgatgttct gctggatatg cacttttccg ggctgacgta caccgtgctc
     4861 agcctgtttt tcagcgatcc ggagatgcat ccgctggatt tcagtctgct gaaccggcgc
     4921 gaggctgacg aagagcctga agatgatgtg ctgatgcaga aagcggcagg gcttgccgga
     4981 ggtgtccgct ttggcccgga cgggaatgaa gttatccccg cttccccgga tgtggcggac
     5041 atgacggagg atgacgtaat gctgatgaca gtatcagaag ggatcgcagg aggagtccgg
     5101 tatggctgaa ccggtaggcg atctggtcgt tgatttgagt ctggatgcgg ccagatttga
     5161 cgagcagatg gccagagtca ggcgtcattt ttccggtacg gaaagtgatg cgaaaaaaac
     5221 agcggcagtc gttgaacagt cgctgagccg acaggcgctg gctgcacaga aagcggggat
     5281 ttccgtcggg cagtataaag ccgccatgcg tatgctgcct gcacagttca ccgacgtggc
     5341 cacgcagctt gcaggggggc agaatccctg gctgatcctg ctgcaacagg gtggtcaggt
     5401 taaggactcc ttcggcggga tgatccccat gttcaggggg cttgccggcg cgatcaccct
     5461 gccgatggtg ggggccacct cgctggcggt ggcgaccggt gcgctggcgt atgcctggta
     5521 tcagggcaac tcaaccctgt ccgatttcaa caaaacgctg gtcctttccg g
//
LOCUS       DAZOZY010000238         5568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZOZY010000238 DAZOZY010000000
VERSION     DAZOZY010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.241"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..297
                     /locus_tag="ACHURQ_003829"
     CDS             <1..297
                     /locus_tag="ACHURQ_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR0825491.1"
                     /translation="AQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPG
                     MTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            307..639
                     /locus_tag="ACHURQ_003830"
     CDS             307..639
                     /locus_tag="ACHURQ_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR0825492.1"
                     /translation="MTSKEIFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            695..1720
                     /locus_tag="ACHURQ_003831"
     CDS             695..1720
                     /locus_tag="ACHURQ_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR0825493.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            1762..2157
                     /locus_tag="ACHURQ_003832"
     CDS             1762..2157
                     /locus_tag="ACHURQ_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR0825494.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDGT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            2169..2522
                     /locus_tag="ACHURQ_003833"
     CDS             2169..2522
                     /locus_tag="ACHURQ_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR0825495.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            2534..3112
                     /locus_tag="ACHURQ_003834"
     CDS             2534..3112
                     /locus_tag="ACHURQ_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0825496.1"
                     /translation="MVIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLPRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            3109..3504
                     /locus_tag="ACHURQ_003835"
     CDS             3109..3504
                     /locus_tag="ACHURQ_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR0825497.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            3512..4252
                     /locus_tag="ACHURQ_003836"
     CDS             3512..4252
                     /locus_tag="ACHURQ_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0825498.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            4268..4690
                     /locus_tag="ACHURQ_003837"
     CDS             4268..4690
                     /locus_tag="ACHURQ_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR0825499.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGSAEPVSAGKCSTVS"
     gene            4672..5106
                     /locus_tag="ACHURQ_003838"
     CDS             4672..5106
                     /locus_tag="ACHURQ_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR0825500.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            5099..>5568
                     /locus_tag="ACHURQ_003839"
     CDS             5099..>5568
                     /locus_tag="ACHURQ_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFR0825501.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSG"
BASE COUNT         1242 a         1438 c         1733 g         1155 t
ORIGIN      
        1 gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg cggcagaaaa cagccgcatt
       61 atggggatcc tcaactgtga ggaggctcac ggacgcgaag aacaggcacg cgtgctggcc
      121 gaaacccccg gtatgaccgt ggaaacggcc cgccgcattc tggccgcagc accacagagt
      181 gcacaggcgc gcagtgacac tgcgctggat cgtctgatgc agggtgcacc ggcaccgctg
      241 gctgcaggta acccggcatc tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg
      301 atgtttatga cgagcaaaga aatctttacc cattaccagc cgctgggcaa cagtgacccg
      361 gcacatacgg caaccgcgcc cggcggattg agtgcgaaag cgcctgcaat gactccgctg
      421 atgctggaca ccgccacccg taagctggtt gcgtgggatg gcaccaccga cggtgctgcc
      481 gttggaattc tggctgttgc tgctgaccag accagcacca cgctgacgtt ctacaagtcc
      541 ggcacgttcc gttatgagga tgtgctctgg ccggaggctg ccagtgacga gacgaaaaaa
      601 cggaccgcgt ttgccggaac ggcaatcagc atcgtttaac ctgacccttc atcactaaag
      661 gccgcctgtg cggctttttt tacgggattt ttttatgtcg atgtacacaa ccgcccagct
      721 gctggcggca aatgagcaga aatttaagtt tgatccgctg tttctgcgtc tctttttccg
      781 tgaaagctat cccttcacca cggagaaagt ctatctctca caaattccgg gactggtaaa
      841 catggcgctg tacgtttcgc cgattgtttc cggtgaggtt atccgctccc gtggcggctc
      901 cacctctgaa tttacaccgg gatatgtcaa acccaagcat gaggtgaatc cgcagatgac
      961 cctgcgtcgt ctgccggatg aagatccaca gaatctggcg gacccggctt accgccgccg
     1021 tcgcatcatc ctgcagaaca tgcgagacga agagctggcc attgctcagg tcgaagagat
     1081 gcaggccgtt tctgccgtgc ttaagggcaa atacaccatg accggtgaag ccttcgatcc
     1141 ggttgaagtg gatatgggcc gcagtgtggc gaacaacatc acgcagtccg gcggtacgga
     1201 gtggagcaag cgtgacaagt ccacgtatga cccgaccgac gatatcgaag cctacgcgct
     1261 gaacgccagc ggtgtggtga atatcatcgt gtttgatccg aaaggctggg cgctgttccg
     1321 ttccttcaaa gccgtcaagg agaagctgga tacccgtcgc ggctctaatt ccgagctgga
     1381 gacagcggta aaagacctgg gcgaagcggt gtcctataag gggatgtatg gcgatacggc
     1441 gatcgtcgtg tattccggac agtatgtgga aaacgacgtc aaaaagaact tccttccgga
     1501 caacacgatg gtgctgggga acactcaggc acgcggtctg cgcacctatg gctgcattca
     1561 ggatgcggac gcacagcgcg aaggtattaa cgcctctgcc cgctacccga aaaactgggt
     1621 gaccaccggc gatccggcgc gtgagttcac catgattcag tcagcaccgc tgatgctgct
     1681 ggctgaccct gatgcgttcg tgtccgtaca actggcgtaa tcatggccct tcggggccat
     1741 tttctctctg tggaggagtc catgacgaaa gatgaactga ttgcccgtct tcaggtgctg
     1801 ggtgagcaac tgaaccgtga tgtcagcctg acggggacga aagaagaact ggcactccgt
     1861 gtggcagagc tggaagagga gcttgatggc acggatgacg ctgccggtca ggacacatct
     1921 gtcagcccgg aaaatgcgct gaccggacat gaaaatgagg ttgtatcagc gcagccggat
     1981 accgtgactg atacggctga tctggtcacg gttgtggcac tggtgacgct gcatactgat
     2041 gcacttcacg ccacgcggga tgaggctgtg gcatttgtgc tgccgggaac ggcgttccgt
     2101 gtctctgccg gtgtggcagc tgaaatgaca gaacgtggcc tggccagaat gcaataacgg
     2161 gaggcgctgt ggctgatttc gataacctgt tcgatgctgc cattgcccgc gccgatgaaa
     2221 cgatacgcgg gtacatggga acgtcagcca ccatgacatc cggtgagcag tccggcgcag
     2281 taatacgtgg tgtttttgat gaccctgaaa atatcagcta tgccggacag ggcgtgcgcg
     2341 ttgaaggctc cagcccgtcc ctgtttgtcc ggactgatga ggtgcggcag ctgcggcgtg
     2401 gagacacgct gaccatcggt gaggaaaact tctggataga ccgggtatcg ccggatgatg
     2461 gcggaagctg tcatctctgg cttgggcgtg gcgttccgcc tgccgttaac cgtcggcgtt
     2521 gaaaggggga tgtatggtca taaaaggtct tgagcaggcc gttgaaaacc tcagccgtat
     2581 cagcaaaacg gcggttcccg gtgccgccgc aatggccatt aaccgcgttg cgtcatccgc
     2641 gatatcgcag tcggcgtcac aggttgcccg tgagacaaag gtacgccgga aactggtaaa
     2701 ggaaagggcc aggctgaaaa gggccacggt caaaaatccg caggccagaa tcaaggttaa
     2761 ccggggggat ttgcccgtaa taaagctggg taacgcgcgg attgtcctgc cccgacgcag
     2821 gcgtcgtaaa aaggggcagc gttcagccct gaaaggtggc ggcagcgtgc ttgtggtggg
     2881 aaaccgtcgt attcccggcg cgtttattca gcaactgaaa aatggccgct ggcatgttat
     2941 gcagcgtgtg gccgggaaaa atcgttaccc cattgatgtg gtgaaaattc cgatggcggt
     3001 gccgctgacc acggcgttta aacagaatat tgagcggata cggcgtgagc gtcttccgaa
     3061 agagctgggc tatgcgctgc agcatcaact gaggatggta ataaagcgat gaaacatact
     3121 gaactccgtg cagccgtact ggatgcactg gagaagcatg acaccggggc gacgtttttt
     3181 gatggtcgcc ccgctgtttt tgatgaggcg gattttccgg cagttgccgt ttatctcacc
     3241 ggcgctgaat acacgggcga agagctggac agcgatacct ggcaggcgga gctgcatatc
     3301 gaagttttcc tgcctgctca ggtgccggat tcagagctgg atgcgtggat ggagtcccgg
     3361 atttatccgg tgatgagcga tatcccggca ctgtcagatt tgatcaccag tatggtggcc
     3421 agtggctatg actaccggcg cgacgatgat gcgggcctgt ggagttcagc cgatctgacg
     3481 tatgtcatta cctatgaaat gtgaggacga tatgcctgta ccaaatccag caataccggt
     3541 gaaaggtgcc ggaaccaccc tgtgggttta taacgggagc ggcgaccctt atgcgaaccc
     3601 gctttcagac gttgactggt cgcgtctggc taaagttaaa gacctgacgc ccggcgaact
     3661 gaccgctgag tcctatgacg acagttatct cgatgatgaa gatgcggact ggactgcgac
     3721 cgggcagggg cagaaatctg ccggagatac cagcttcacg ctggcgtgga tgcccggaga
     3781 gcaggggcag caggcgctgc tggcgtggtt taatgaaggg gatacccgtg cctataaaat
     3841 ccgcttcccg aacggcacgg tcgatgtgtt ccgcggctgg gtcagcagta tcggtaaggc
     3901 ggtgacggcg aaggaagtga tcacccgtac ggtgaaagtc accaacgtgg gacgtccgtc
     3961 gatggcagaa gatcgcagca cggtaacagc ggcaaccggc atgaccgtga cgcctgccag
     4021 cacttcggtg gtgaaagggc agagcaccac gctgaccgtg gcattccagc cggaaggcgc
     4081 aaccgacaag agcttccgtg cggtgtcagc ggataaaaca aaagccaccg tgtcggtcag
     4141 tggtatgacc atcaccgtga aaggtgttgc tgcaggcaag gtcaacattc cggttgtatc
     4201 cggtaatggt gagtttgctg cggttgcaga aattaccgtc accgccagtt aatccggaga
     4261 gtcagcgatg ttcctgaaaa ccgaatcatt tgaacataac ggtgtgaccg tcacgctttc
     4321 tgaactgtca gccctgcagc gtattgagca tctcgccctg atgaaacggc aggcagaaca
     4381 ggcggagtca gacagcaacc ggaagtttac tgtggaagac gccatcagaa ccggtgcttt
     4441 tgtggtggcg atgtccctgt ggcataacca tccgcagaag acaaagcagc cttccatgaa
     4501 tgaagccgtt aaacagattg agcaggaagt gcttaccacc tggcccacgg aggcaatttc
     4561 tcatgctgaa aacgtggtgt accggctgtc cggtatgtat gagtttgtgg tgaatgatgc
     4621 ccctgaacag gcagaggacg ccgggtctgc agagcctgtt tctgcgggaa agtgttcgac
     4681 ggtgagctga gttttgccct gaaactggcg cgcgaaatgg ggcgacccga ctggcgtgcc
     4741 atgcttgccg ggatgtcatc cacggagtat gccgactggc accgctttta cagtacccat
     4801 tattttcatg atgttctgct ggatatgcac ttttccgggc tgacgtacac cgtgctcagc
     4861 ctgtttttca gcgatccgga tatgcatccg ctggatttca gtctgctgaa ccggcgtgag
     4921 gctgacgaag agcctgaaga tgatgtgctg atgcagaaag cggcagggct tgccggaggt
     4981 gtccgctttg gcccggacgg gaatgaagtt atccctactt ccccggatgt ggcggacatg
     5041 acggaggatg acgtaatgct gatgacagta tcagaaggga tcgcaggagg agtccggtat
     5101 ggctgaaccg gtaggcgatc tggtcgttga tttgagtctg gatgcggcca gatttgacga
     5161 gcagatggcc agagtcaggc gtcatttttc cggtacggaa agtgatgcga aaaaaacagc
     5221 ggcagttgtt gaacagtcgc tgagccgaca ggcgctggct gcacagaaag cggggatttc
     5281 cgtcgggcag tataaagccg ccatgcgtat gctgcctgca cagttcaccg acgtggccac
     5341 gcagcttgca ggcgggcaaa gtccgtggct gatcctgctg caacagggtg gtcaggttaa
     5401 ggactccttc ggcgggatga tccccatgtt cagggggctt gccggtgcga tcaccctgcc
     5461 gatggtgggg gccacctcgc tggcggtggc gaccggtgcg ctggcgtatg cctggtatca
     5521 gggcaactca accctgtccg atttcaacaa aacgctggtc ctttccgg
//
LOCUS       DAZOZY010000239         5541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZOZY010000239 DAZOZY010000000
VERSION     DAZOZY010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.242"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHURQ_003840"
     CDS             complement(<1..1369)
                     /locus_tag="ACHURQ_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR0825502.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHURQ_003841"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHURQ_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR0825503.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHURQ_003842"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHURQ_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR0825504.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHURQ_003843"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHURQ_003843"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR0825505.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHURQ_003844"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHURQ_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR0825506.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZOZY010000240         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZOZY010000240 DAZOZY010000000
VERSION     DAZOZY010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.243"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHURQ_003845"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHURQ_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR0825507.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHURQ_003846"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHURQ_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR0825508.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHURQ_003847"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHURQ_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR0825509.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHURQ_003848"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHURQ_003848"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR0825510.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHURQ_003849"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHURQ_003849"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR0825511.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHURQ_003850"
     CDS             4447..4812
                     /locus_tag="ACHURQ_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR0825512.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHURQ_003851"
     CDS             4962..5480
                     /locus_tag="ACHURQ_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR0825513.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1391 c         1241 g         1376 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZOZY010000241         5512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZOZY010000241 DAZOZY010000000
VERSION     DAZOZY010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.244"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(320..1108)
                     /gene="phoH"
                     /locus_tag="ACHURQ_003852"
     CDS             complement(320..1108)
                     /gene="phoH"
                     /locus_tag="ACHURQ_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR0825514.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1729..3000)
                     /gene="efeB"
                     /locus_tag="ACHURQ_003853"
     CDS             complement(1729..3000)
                     /gene="efeB"
                     /locus_tag="ACHURQ_003853"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR0825515.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3006..4133)
                     /gene="efeO"
                     /locus_tag="ACHURQ_003854"
     CDS             complement(3006..4133)
                     /gene="efeO"
                     /locus_tag="ACHURQ_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR0825516.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4191..5021)
                     /gene="efeU"
                     /locus_tag="ACHURQ_003855"
     CDS             complement(4191..5021)
                     /gene="efeU"
                     /locus_tag="ACHURQ_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR0825517.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
BASE COUNT         1377 a         1514 c         1306 g         1315 t
ORIGIN      
        1 acagtcagtc aaagtatagc ccattgttga ttaaacctca taagcaaaat tggcaccaga
       61 tctcctgtac aggttgaatg gccgaaaaaa gataggcaga tgctcatttt tttggcttct
      121 ggaagaaaaa ctctccgcgc tggcagtatt acactggtct gatgctatga cgaaaaatgc
      181 ctggtaagag tatctaaacc agactccagt tcagcttatc aacacgctca aagtcgcccc
      241 cctgaacgct aacctgaagt gaaaatctgt tgaaataaaa caaagcccgg ttcgcccggg
      301 ctctgcaccg ataacacact taactgtagg catgcagcgt acgttggcaa agtgccgaac
      361 gtacgcagtc ctctttaccg aaccggacga tcccaaccat ttcatcttct tcgaaacgtt
      421 ccagcgcgtc acttaatccg gagcacacgc cgcgaggcaa atcgcattgc gtgatatcac
      481 cgttgacgat aaccgtcacg ttctccccga ggcgggttaa aaacattttc atttgcgcgg
      541 cagtcacatt ctgcgcctcg tcaagaatga cgactgcatt ttcaaaggta cgtccacgca
      601 tataggcgaa cggcgcaatt tccaccttcc ctatttccgg tcgcaggcag tactgcataa
      661 aggaagcccc taagcgccgg accagcacgt cgtagaccgg gcgaaaatag ggagcaaact
      721 tttctgcgat atctccaggt aagaagccaa gatcttcatc ggcttgcaga actggacggg
      781 tgacgataat cctgtcgaca tccttatgta tcagggcctc tgccgctttt gctgcgctga
      841 tccaggtttt tccgcccccg gcttcgcccg tggcgaatat cagctgctta ctctcaatag
      901 ccttcagata gtgcaattgc gcttcatttc gcgcgaggat gggcgaagta tcgcgactgt
      961 cgcgggccat accaatggct tctacgccgc ccatctgcac aagcgaggtg accgattctt
     1021 cttcacgctg cttatggctg cgcgaatccc gtctcagcac acgttttgcc tcgcgacgag
     1081 ctttgatcac tgctttttgt cttcccatag agagcacctt gagttgtttg tattcatcac
     1141 acgcgccgtt ggcagcgcga ttatgcgcac gaacatcaga gggttggctt ccttgtaagc
     1201 catagtttgc ttttggataa aatgccgaaa aacggctacg cgcaccgttt acggcgtcgg
     1261 taacacatga aaagaaagga tgaggttgaa aatgcaaagt gacgagatga ctaccggagg
     1321 agaaaactcc gcgagtggtg gcgcgttgat tatctaaaac atgtccagta caggacgtta
     1381 ccatccgcga tctccatagt gactgactat cactgccggg aacttccgct gctacttaat
     1441 aagtacaaca gatctcgcat ttattgcaac aatatattta cttatattta actataaaac
     1501 actatttcag tgacattagt ttctactgga aagatgacag agtgatgaca gtgatgaaaa
     1561 aagctgtgtg ctttcagcag gatttgcatc aggcatcagt gcacgatcgc cggatgcggc
     1621 gtgaacgcct tatccagcct accaaacccg cactgattgt aggcatgata agacgcgcaa
     1681 gcgtcgcatc aggcatctgc gcacaaatgc cttatccgcc taaaaacatt aaacccgcaa
     1741 taacgcgctt ccgagataat cgttcgcgtc cttcactccc ggcagcgaaa aaaaataacc
     1801 gccgccgata ggtttaacgt attcctccag cgcttcgcca ttgagccttt tttgtactgt
     1861 caggaagcct ttttccagat cgtgttggta gcagacaaac agcaacccca tatccagttg
     1921 tccggagttg gtgaccccca acgaataact gtagccgcga cgcagcatca ggctggactc
     1981 actctccgcc gtgcggggat tcgccagccg gatatggctg tccagcgcaa tcaccttccc
     2041 ttccgggtcg ctggcgtaat caggcacatc atgctcatgc tgcattccca gcggcgcacc
     2101 ggtttgctta tcacggccaa aaatcgtctg ctgttctttc agcggcgttc tgtcccaaaa
     2161 ttccactcga aactgaatca agcgtactgc ctgatagctg ccaccgattg tccacgcagg
     2221 ctcctgctga tctgccgtta cccacaccac tttttgcatc aacttatcat tctggctatc
     2281 gggattggca gtgccgtctt tgaaacccag caaattaatc ggcgtctctt tgcctttact
     2341 acgcgacacg tgatcggaaa taaacccttc ccgcttccag cgcacactga gcaaatccgg
     2401 cgtgtgtttg atgatatcgc gcagcgcatg gataaccgtg tcctgggtgt tggcgcaaat
     2461 ctgtagcaac acatcaccat gacataacgc cgcatccagc gagtcgttgg ggaaacgcgt
     2521 catcttctgc agcttttttg gcatctgtgg cgcaaggcca aagcgctcat caaacaatga
     2581 gtgacccacc gataacgtga tggtgagatt atcgggcgca atgtagccgc caagaatgcc
     2641 ggaatcgagt ggtggcaggc gcggatttgg cgtttctggt gctgctccgc cctgagtcag
     2701 aaaagcaaaa cgctgagtca acaagcgaaa caaccgctca agatcggctt tatcgctggc
     2761 aagcacatca aacgccacca gcatcattgc ggcctgttgt ggcgtcagga tccctgcctg
     2821 atgctcacca taaaacggct gtttctcatt gcgtgcatcc ggtgaaagcg tgcccggtgc
     2881 actttgcgtt ttttgtgcat gagcgaccgg acaacttccc gccagcgcca gcgcaccaat
     2941 ccctttcagt aaacgtcggc gtgacggttc attcacgccg ttttcatctt tatactgcat
     3001 aacgcttaat ccagtcccag cacaccgcga agttgcgcca gatcttccgc cagcgcagta
     3061 atcggtcctt tcagtgcatt ccggtcggca tcggtcaatt tgtcgtaggt ttcaaaaccg
     3121 tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc atcgactttt
     3181 gccagcagtt ccgggttggc tttttgcagt tgtggacgca gcaaatcgac aattttctgc
     3241 gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg atcttcttca
     3301 ccgctaattt tgctggctgc cacttcctca atcagtccgg ctgcgccgcc gaccactttt
     3361 gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc tgtataaagc
     3421 tgctcagcgt actgatccat ccctttggtg gtgttgtcgc caaacaatgc tttttccaga
     3481 cggtggaaac cagtgaattt tgggtcggcg gctttttgct cgtaatcatc ttcacgggcg
     3541 tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatgcg ctcatagtgc
     3601 tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt aatcgcgtcg
     3661 gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata cgctttatat
     3721 gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc cgccgttgcc
     3781 tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt catatcgtat
     3841 tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat attttcccgc
     3901 tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg gctatggttc
     3961 tgaataatga actgtgtttt cccggcgtta accgtaatgg tcatcggttc gcactgctta
     4021 tccgtcacgg tcactttgac ctgcggcaca tcagcggcgt tagccataaa agcagaagaa
     4081 aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt catgacgaga
     4141 ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg ttacgcggag
     4201 cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc cgggatgaga
     4261 taaataaacc agacggcgac ttcgctgacg ctcggtgctt cctgatagcc aaaaatccct
     4321 tccatcagcg tgccaaacag cgagtgagtt gagagcactg cgctcatatc gaaggcgatt
     4381 tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc agcagccagc
     4441 cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc aagattgagg
     4501 cgaataccgc cccagtagag caggaagcct agcaccacgg cagtagcaag accgagcatt
     4561 gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag gaaaaagacc
     4621 gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc ccagccatga
     4681 tgatttccac gctgcaatgc gctatcgact gcctgctcca gttgtacttt gacgttgcgc
     4741 gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat caccgccacg
     4801 ataccttcaa acagttcctg ttctttttgc ggaaattcgc cggtggtttc gttaatgaag
     4861 atacccaagc ccaggcacaa cgcagcggca agcaacacgc caatccacat cacgccaatc
     4921 catcggcctc gctgggtacg cttaagatag ctggcaatca aactgacaat cagcgcggct
     4981 tcaagtcctt cgcgcaacat aatgagaaac ggaacaaaca tgccacccac ccttaaacgt
     5041 ttttctgagt caatccatgc aaagaaaagt aaatcgcagt gatagcgatt atcattacgg
     5101 caaagaaaaa tacaagcgga atgtttataa aatgatgttg gaatgtaaac aaagagaggt
     5161 aataccaggg gatataaacg attattccag gtacaaagat gggattgttt acaacgagcc
     5221 atgtgttctt gccagatgcc gcgtaaacgc cttatccagc cgacaaaact gtccagactc
     5281 aatgcatcac agcttccatg taaaactgat aagcgggtgt atcaagcaat ttcgtccatc
     5341 cgcgattatc aaatctaact ttttgcccgg aacataataa cagcgcgggt gttggtggcg
     5401 tcaaaatcgc tgaaacgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg
     5461 cgcgtcttac aggacgttac ctcgcgcccg acccggtagc cgaaggggat aa
//
LOCUS       DAZOZY010000242         5512 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZOZY010000242 DAZOZY010000000
VERSION     DAZOZY010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.245"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..2062
                     /locus_tag="ACHURQ_003856"
     CDS             <1..2062
                     /locus_tag="ACHURQ_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0825518.1"
                     /translation="LVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVAR
                     FSSASGVEVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGAL
                     QAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIK
                     AEAAFKKADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQ
                     QQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAA
                     KKDYEATLKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRD
                     LWKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALA
                     QQADKFAQQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKK
                     TWAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQ
                     NWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHF
                     ATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPA
                     QMRRAEGINFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFS
                     GGGR"
     gene            2059..2388
                     /locus_tag="ACHURQ_003857"
     CDS             2059..2388
                     /locus_tag="ACHURQ_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR0825519.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            2388..3086
                     /locus_tag="ACHURQ_003858"
     CDS             2388..3086
                     /locus_tag="ACHURQ_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR0825520.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            3092..3835
                     /locus_tag="ACHURQ_003859"
     CDS             3092..3835
                     /locus_tag="ACHURQ_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR0825521.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            3832..4404
                     /locus_tag="ACHURQ_003860"
     CDS             3832..4404
                     /locus_tag="ACHURQ_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR0825522.1"
                     /translation="MKTGAEAIRAMATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            4465..>5512
                     /locus_tag="ACHURQ_003861"
     CDS             4465..>5512
                     /locus_tag="ACHURQ_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR0825523.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCM"
BASE COUNT         1265 a         1359 c         1801 g         1087 t
ORIGIN      
        1 gctggtcctg tccagagccg ggcaggcggc agggctgacg tttaaccaga ccagcgagtc
       61 actcagcgca ctggttaagg cgggggtaag cggtgaggct cagattgcgt ccatcagcca
      121 gagtgtggcg cgtttctcct ctgcatccgg cgtggaggtg gacaaggtcg ctgaagcctt
      181 cgggaagctg accacagacc cgacgtcggg gctgacggcg atggcacgcc agttccataa
      241 cgtgacggcg gagcagattg cgtatgttgc tcagttgcag cgttccggcg atgaagccgg
      301 ggcattgcag gcggcgaacg aggccgcaac gaaagggttt gatgaccaga cccgccgcct
      361 gaaagagaac atgggcacgc tggagacctg ggcagacagg actgcacgtg cattcaaatc
      421 catgtgggat gcggtgctgg atattggtcg tcctgatacc gcgcaggaga tgctgattaa
      481 ggcagaggct gcgtttaaga aagcagacga catctggaat ctgcgcaagg atgattattt
      541 cgttaacgat gaagcacggg cgcgttactg ggatgatcgt gaaaaaaaac gcctggagcg
      601 ggatgcagcc caaaagaggg tagatcaaca gcgtcaacag gacaaaaatg cgcagcagca
      661 gagcgatacc gaagcgtcac ggctgaaata taccgaagag gcgcagaagg cttacgaacg
      721 gctgcagacg ccgctggaga aatataccgc ccgtcaggaa gaactgaaca aggcactgaa
      781 agacggaaaa atcctgcagg cagattacaa cacgctgatg gcggcggcga aaaaggatta
      841 tgaagcgacg ctgaaaaagc cgaaacagtc cggcgtgaag gtgtatgcgg gcgatcgtca
      901 ggaagacagt gctcatgcgg ccctgctgac gcttcaggca gaactccgga cgctggagaa
      961 gcatgccgga gcgaatgaga aaatcagcca gcagcgccgg gatttgtgga aggcagaaag
     1021 tcagttcgcg gtactggagg aggcggcaca acgtcgccag ctgtccgcac aggagaaatc
     1081 cctgctggcg cataaagacg agacgctgga gtacaaacgc cagctggctg cacttggtga
     1141 caaggttacg tatcaggagc acctgaatgc gctggcgcag caggcggata aattcgcaca
     1201 gcagcaacgg gcaaaacggg cagccattga tgcgaaaaac cgggggctga ctgaccggca
     1261 ggcagcgcgg gaagccacgg aacagcgcct gaaggaacag tatggcgata atcctctggc
     1321 gctgaataac gtcatgtcag agcagaaaaa gacctgggcg gctgaagacc agcttcgcgg
     1381 gagctggatg gcaggcctga agtccggctg gagtgagtgg gaagagagcg ccacggacag
     1441 tatgtcacag gttaaaagtg cagccacgca gacctttgat ggtattgcac agaatatggc
     1501 ggcgatgctg accggcagtg aacagaactg gcgcagcttc acccgctccg tgctgtccat
     1561 gatgacagaa attctgctta agcaggcaat ggtggggatt gtcgggagta tcggcagcgc
     1621 cattggcggg gctgttggtg gcggcgcatc cgcgtcaggt ggtacagcca ttcaggccgc
     1681 tgcggcgaaa ttccattttg caaccggggg atttacggga accggcggca aatatgagcc
     1741 agcggggatt gttcaccgtg gtgaatttgt cttcacgaag gaggcaacca gccggattgg
     1801 cgtggggaat ctctaccggc tgatgcgcgg ctatgcggaa ggtggttatg tgggcggtgc
     1861 cggaagtccg gcgcagatgc ggcgggctga aggcattaat tttaatcaga acaatcacgt
     1921 ggtgattcag aacgacggta cgaatggtct gccaggtcca cagatgatga aggccgtgta
     1981 tgacatggcc cgcaagggtg cccgtgatga aattcagaca cagatgcgtg atggtggcct
     2041 gttctccgga ggtggacgat gaaaaccttc cgctggaaag tgaaacccgg tatggatgtg
     2101 gcttcggccc cttctgtaag aaaggtgcgc tttggtgatg gctattccca gcgagcgcct
     2161 gccgggctga atgccaacct gaaaacgtac agcgtgacgc tttctgtccc ccgtgaggag
     2221 gccatggtac tggagtcgtt tctggaagag cacgggggct ggaaagcctt tctgtggacg
     2281 ccgccttatg agtggcggca gataaaggtg acctgcgcaa aatggtcgtc gcgggtcagt
     2341 atgctgcgtg ttgagttcag cgcagagttt gaacaggtgg tgaactgatg caggatatcc
     2401 ggcaggaaac actgaatgaa tgcacccgtg cggagcagtc ggccagcgtg gtgctctggg
     2461 aaatcgatct gacagaggtc ggtggagaac gttatttttt ctgtaatgag cagaacgaaa
     2521 aaggtgagcc ggtcacctgg caggggcgac agtatcagcc gtatcccatt caggggagtg
     2581 gttttgaact gaatggcaaa ggcaccagta cgcgccccac gctgatggtt tctaacctgt
     2641 acggtatggt caccgggatg gcggaagatc tacagagtct ggtcggcgga acggtggtcc
     2701 ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga aacagtgacg
     2761 ccgatccgga gcaggaggtg atcagccgct ggcgcattga gcagtgcagc gaactgagcg
     2821 cggtgagtgc ctcttttgta ctgtccacgc cgacggaaac agatggcgct gtttttccgg
     2881 gacgtatcat gctggccaac acctgcacct ggacctatcg cggcgatgag tgcggttatc
     2941 acggtccggc ggtcgcggat gaatatgacc agccaacgtc cgacatcacg aaggataaat
     3001 gcagcaaatg cctgagtggc tgtaagtttc gcaataacgt cggcaacttt ggcggcttcc
     3061 tttccattaa caaactttcg cagtaaatcc catgacagag acagaatcag cgattctggc
     3121 gcacgcccgg cgatgtgctc cagcggagtc gtgcggcttc gtggtaagaa cgccggaggg
     3181 ggaaagatat ttcccctgcg tgaatatctc cggtgagccg gaggcgtatt tccgtatgtc
     3241 gccggaagac tggctgcagg cagagatgca gggtgagatt gtggcactgg tccacagcca
     3301 ccccggtggt ctgccctggc tgagtgaggc cgaccggcgg ctgcaggtgc agagtgattt
     3361 gccgtggtgg ctggtctgcc ggggggcgat tcacaagttc cgctgtgtgc cacatcttac
     3421 cgggcggcgc tttgagcacg gggtgacgga ctgttacacg ctgttccggg atgcttacca
     3481 tctggcgggg attgagatgc cggattttca tcgtgaggat gactggtggc gtcacggtca
     3541 gaatctctat ctggataatc tggaggccac agggctgtat caggtgccgt tgtcagcggc
     3601 gcagccgggc gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc atgccgccat
     3661 ttactgtggt gacggcgagc tgctgcacca tattcctgaa caactgagca aacgagagag
     3721 gtataccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg catggcacgc
     3781 atctgccttt acggggattt gcaacgattt ggccgccgca tcgaccttcg tgtgaaaacg
     3841 ggtgccgaag ccatccgggc gatggccaca cagctccctg cgtttcgtca gaagctgagc
     3901 gacggctggt atcaggtgcg tattgccggg caggatgtca gcacgtccgg attaacggcg
     3961 cagttacatg aggctctgcc tgacggcgct gtgattcata ttgttcccag agtcgccggg
     4021 gccaagtcag gtggtgtatt ccagattgtc ctgggagcag ccgccattgc cggatcattc
     4081 tttaccgccg gagccaccct tgcagcatgg ggggctgcca ttggtgccgg tggtatgacc
     4141 ggcattctgt tttctctcgg tgccagtatg gtgctcggcg gtgtggctca gatgctggca
     4201 ccgaaagcca gaactccccg cacacagaca acggataacg gtaagcagaa catctatttc
     4261 tcctcactgg ataacatggt tgcccagggc aatgttatgc ctgttctgta tggtgaaatg
     4321 cgcgtggggt cacgtgtggt atctcaggag atcagcacgg cagacgaagg ggacggtggt
     4381 caggttgtgg tgattggtcg ctgatgcaaa atgttttatg tgaaatcgcc tccgggcggt
     4441 tttatcgttt atggagcatg acgaatgggt aaaggcagca gtaaggggca taccccgcgc
     4501 gaagcgaagg acaacctgaa gtccacgcag ctgctgagtg tgatcgatgc catcagcgaa
     4561 gggccggttg aaggtccggt ggatggatta aaaagcgtgc tgctgaacag tacgccggtg
     4621 ctggacagtg aggggaatac caacatctcc ggcgtcacgg tggtgttccg ggcaggtgag
     4681 caggagcagt caccgccgga gggatttgaa tcctccggct ccgagacggt gctgggtacg
     4741 gaagtgaaat atgacacgcc gatcacccgg accatcacgt cggcaaacat tgaccgactg
     4801 cgttttacct tcggtgtaca ggcactggtg gaaaccacct caaagggaga caggaatccg
     4861 tctgaagttc gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac
     4921 atcaccatta agggcaaaac cacctcgcag tatctggcct cggtggtggt ggataacctg
     4981 ccgccgcgcc cgtttaatat ccggatgcgc agaatgacgc cggacagcac cacagaccag
     5041 ctgcagaaca aaacgctctg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac
     5101 ccgaacacgg cactggtcgg cgtacaggtg gattcggagc agttcggcag ccagcaggtg
     5161 agccgtaatt atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taatccgcag
     5221 acgcggcaat acagcggtat ctgggacgga acgttaaaac cggcatacag caacaacatg
     5281 gcctggtgtc tgtgggatat gctgacccat ccgcgctatg gcatggggaa acgtcttggt
     5341 gcggcagatg tggataaatg ggcgctgtat gtcatcggcc agtactgtga ccagtcggtg
     5401 ccggacggct ttggcagcac ggagccgcgc atcacctgta atgcctggct gaccacacag
     5461 cgcaaggcgt gggatgtgct cagtgatttc tgctcggcga tgcgctgtat gc
//
LOCUS       DAZOZY010000243         5477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZOZY010000243 DAZOZY010000000
VERSION     DAZOZY010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.246"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            500..586
                     /gene="leuL"
                     /locus_tag="ACHURQ_003862"
     CDS             500..586
                     /gene="leuL"
                     /locus_tag="ACHURQ_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR0825524.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            679..2250
                     /gene="leuA"
                     /locus_tag="ACHURQ_003863"
     CDS             679..2250
                     /gene="leuA"
                     /locus_tag="ACHURQ_003863"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR0825525.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2250..3341
                     /gene="leuB"
                     /locus_tag="ACHURQ_003864"
     CDS             2250..3341
                     /gene="leuB"
                     /locus_tag="ACHURQ_003864"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR0825526.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3344..4744
                     /gene="leuC"
                     /locus_tag="ACHURQ_003865"
     CDS             3344..4744
                     /gene="leuC"
                     /locus_tag="ACHURQ_003865"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR0825527.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4755..5360
                     /gene="leuD"
                     /locus_tag="ACHURQ_003866"
     CDS             4755..5360
                     /gene="leuD"
                     /locus_tag="ACHURQ_003866"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR0825528.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5409..>5477)
                     /locus_tag="ACHURQ_003867"
     CDS             complement(5409..>5477)
                     /locus_tag="ACHURQ_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825529.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         1419 a         1432 c         1418 g         1208 t
ORIGIN      
        1 gccgtggaat gagtcattta ccacagcata agaatccata atgtttttgt tgcgaaaaca
       61 atctaatcat aactaccgcg aatactcaat catctacaaa atggattaaa tgtgcttatt
      121 taataattaa tttatatatt taatgcatta attcttaaca ttaattgatc aataatattc
      181 accaaatcaa tatcaaaaaa aatcgcaaaa catataattc aatacaaatc atcaggatag
      241 gttttgcaac gcgtgcattt tgtccccttt ttcctcgttg attagatgca aaaatttatg
      301 ctgaaatatg tcaaccgatg aaaagcgtcg gtagttaagc agaaattaat atcgcttact
      361 ttaaccaccg cagcacaatt agctaatttt acggatgcag aactcacgct ggcgggacgt
      421 ttttattgcg tcagggttga catccgtttt tgtatccagt aactctaaaa gcatatcgca
      481 ttcatctgga gctgatttaa tgactcacat cgttcgcttt atcggtctac tactactaaa
      541 cgcatcttct ttgcgcggta gacgagtgag cggcatccag cattaagcca gcacgcagtc
      601 aaacaaaaaa cccgcgccat tgcgcgggtt tttttatgcc cgaagcgagg cgctctaaaa
      661 gagacaagga cctaaaccat gagccagcaa gtcattattt tcgataccac attgcgcgac
      721 ggtgaacagg cgttacaggc aagcttgagt gtgaaagaaa aactgcaaat tgcgctggcc
      781 cttgagcgta tgggtgttga cgtgatggaa gtcggtttcc ccgtctcttc gccgggcgat
      841 tttgaatcgg tgcaaaccat cgcccgccag gttaaaaaca gccgcgtatg tgcgttagct
      901 cgctgcgtgg aaaaagatat cgacgtggcg gccgaatccc tgaaagtcgc cgaagccttc
      961 cgtattcata cctttattgc tacctcccca atgcacatcg ccaccaagct gcgcagcacc
     1021 ctggacgagg tgatcgaacg cgctatctat atggtgaaac gcgcccgtaa ttacaccgat
     1081 gatgttgaat tttcttgcga agatgccggg cgtacaccca ttgccgatct ggcgcgagtg
     1141 gtcgaagcgg cgattaatgc cggtgccacc accatcaaca ttccggacac cgtgggctac
     1201 accatgccgc ttgagttcgc cggaatcatc agcggcctgt atgaacgcgt acctaacatc
     1261 gacaaagcca ttatttccgt acatacccac gacgatttag gcctggcggt cggcaactca
     1321 ctggcggcgg tacatgccgg tgcacgccag gtggaaggtg caatgaacgg gatcggcgag
     1381 cgtgccggta actgttcgct ggaagaagtc atcatggcga ttaaagttcg taaggatatt
     1441 ctcaacgtcc acaccgccat taatcaccag gagatatggc gcaccagcca gttagttagc
     1501 cagatttgta atatgccgat cccggcaaac aaagccattg ttggcagcag cgcattcgct
     1561 cactcctccg gtatccacca ggatggcgtg ctgaaaaacc gcgaaaacta cgaaatcatg
     1621 acaccagaat ctattggtct gaaccaaatc cagctgaatc tgacctctcg ttcggggcgt
     1681 gcggcggtga aacatcgcat ggatgagatg gggtataaag aaagtgaata taatttagac
     1741 aatttgtacg atgctttcct gaagctggcg gacaaaaaag gtcaggtgtt tgattacgat
     1801 ctggaggcgc tggccttcat cggtaagcag caagaagagc cggagcattt ccgtctggat
     1861 tacttcagcg tgcagtctgg ctctaacgat atcgccaccg ccgccgtcaa actggcctgt
     1921 ggcgaagaag tcaaagcaga agccgccaac ggtaacggtc cggtcgatgc cgtctatcag
     1981 gcaattaacc gcatcactga ctataacgtc gaactggtga aatacagcct gaccgccaaa
     2041 ggtcacggta aagatgcgct gggtcaggtg gatattgtcg ccaactacaa cggtcgccgc
     2101 ttccacggcg tcggcctggc caccgatatt gtcgagtcct ccgccaaagc catggtgcac
     2161 gtactgaaca atatctggcg tgccgcagaa gtcgaaaaag agttgcaacg caaagctcaa
     2221 cacaacgaaa acaacaagga aaccgtgtga tgtcgaagaa ttaccatatt gccgtattgc
     2281 cgggggacgg tattggtccg gaagtgatga cccaggcgct gaaagtgctg aatgccgtgc
     2341 gcaaccgctt tgcgatgcgc atcaccacca gccattacga tgtaggcggc gcagccattg
     2401 ataaccacgg gcaaccactg ccgcctgcga cggttgaagg ttgtgagcaa gccgatgccg
     2461 tgctgtttgg ctcggtaggc ggcccgaagt gggaacattt accaccagac cagcaaccag
     2521 aacgcggcgc gctgctgcct ctgcgtaagc gcttcaaatt attcagcaac ctgcgcccgg
     2581 caaaactgta tcaggggctg gaagcattct gtccgctgcg tgcagacatt gccgctaacg
     2641 gcttcgacat cctgtgcgtg cgcgaactga ccggcggcat ctatttcggt cagccaaaag
     2701 gccgcgaagg tagcggacaa tatgaaaaag cgtttgatac cgaggtgtat caccgttttg
     2761 agatcgagcg tattgcccgc atcgcgtttg aatctgctcg taaacgtcgc cacaaagtca
     2821 cctcaatcga caaagctaac gtgctgcaat cctctatttt atggcgtgaa atcgtcaacg
     2881 agatcgccac ggaatacccg gatgtcgaac tggcgcatat gtacatcgac aacgccacca
     2941 tgcagctgat taaagatcca tcacagtttg acgttctgct gtgctccaac ctgtttggcg
     3001 acattctgtc tgacgagtgc gcaatgatca ctggctcgat ggggatgttg ccttccgcca
     3061 gcctgaacga gcaaggtttt ggtctgtatg aaccggcagg cggctcagca ccagatatcg
     3121 caggcaaaaa catcgccaac ccgattgcgc aaattctgtc gctggcactg ctgctgcgct
     3181 acagcctgga tgccgatgat gcggcttgcg ccattgaacg cgccattaac cgcgcattag
     3241 aagaaggcat tcgcaccggg gatttagccc gtggcgctgc cgccgttagt accgatgaaa
     3301 tgggcgatat cattgcccgc tatgtggcag aaggggtgta atcatggcta aaacgttata
     3361 cgaaaaatta ttcgacgctc acgtagtgta cgaagccgaa aacgaaaccc cgctgttata
     3421 tatcgaccgc cacctggtgc atgaagtgac ctcaccgcag gcgttcgatg gtctgcgcgc
     3481 ccacggtcgc ccggtacgtc agccgggcaa aaccttcgcc actatggatc acaacgtctc
     3541 tacccagacg aaagacatta atgcctgtgg tgaaatggcg cgcatccaga tgcaggagct
     3601 gattaaaaac tgcaaagaat ttggcgtcga actgtatgac ctgaatcacc cgtatcaggg
     3661 gatcgtccac gtaatggggc cggaacaggg cgtcaccttg ccggggatga ccattgtctg
     3721 cggcgactcg cataccgcca cccacggcgc gtttggcgca ctggcctttg gtatcggcac
     3781 ttccgaagtt gaacacgtac tggcaacgca aaccctgaaa cagggccgcg caaaaaccat
     3841 gaaaattgaa gtccagggca aagccgcgcc gggcattacc gcaaaagata tcgtgctggc
     3901 aattatcggt aaaaccggta gcgcaggcgg caccgggcat gtggtggagt tttgcggcga
     3961 agcaatccgt gatttaagca tggaaggtcg tatgaccctg tgcaatatgg caatcgaaat
     4021 gggcgcaaaa gccggtctgg ttgcaccgga cgaaaccacc tttaactatg tcaaaggccg
     4081 tttgcatgcg ccgaaaggca aagatttcga cgacgccgtt gcctactgga aaaccctgca
     4141 aaccgacgaa ggcgcaactt tcgataccgt tgtcactctg caagcagaag aaatttcacc
     4201 gcaggtcacc tggggcacca atccaggcca ggtgatttcc gtgaacgaca atattcccga
     4261 tccggcttcg tttgccgatc cggttaaacg cgcgtcggca gaaaaagcgc tggcctatat
     4321 ggggctgaaa ccgggcattc cgctgaccga agtggctatc gacaaagtgt ttatcggttc
     4381 ctgtaccaac tcgcgcattg aagatttacg cgcggcagcg gagatcgcca aagggcgaaa
     4441 agtcgcgcca ggcgtgcagg cactggtggt tcccggctct ggcccggtaa aagcccaggc
     4501 ggaagcggaa ggtctggata aaatctttat tgaagccggt tttgaatggc gcttgcctgg
     4561 ctgctcaatg tgtctggcga tgaacaacga ccgtctgaat ccgggcgaac gttgtgcatc
     4621 caccagcaac cgtaactttg aaggccgcca ggggcgcggc gggcgcacgc atctggtcag
     4681 cccggcaatg gctgccgctg ctgctgtgac cggacatttc gccgacattc gcaacattaa
     4741 ataaggagca caccatggca gagaaattta tcaaacacac aggcctggtg gttccgctgg
     4801 atgccgccaa tgtcgatacc gatgcaatca tcccgaaaca gtttttgcag aaagtgaccc
     4861 gtacgggttt tggcgcgcat ctgtttaacg actggcgttt tctcgatgaa aaaggccaac
     4921 agccaaaccc ggacttcgtg ctgaacttcc cgcagtatca gggcgcttcc attttgctgg
     4981 cacgagaaaa cttcggctgt ggctcctcgc gtgagcacgc gccctgggca ttgaccgact
     5041 acggctttaa agtggtgatt gcgccgagtt ttgctgacat cttctacggc aatagcttta
     5101 acaaccagct gctgccggtg aaattaagcg atgcagaagt ggacgaactg tttgcgctgg
     5161 tgaaagccaa tccggggatc catttcgacg tggatctgga agcgcaagag gtgaaagcgg
     5221 gagagaaaac ctatcgcttt accatcgatg ccttccgccg ccactgcatg atgaacggtc
     5281 tggacagtat tgggcttacc ttgcagcacg acgacgccat tgccgcttat gaagcaaaac
     5341 aacctgcgtt tatgaattaa tccccttgcc cggtcaaatg accgggcttt ccgctatcgt
     5401 ccacgtcatc aaacgtcttt aacctttgcg gttaaaaata atgcgacaac agaaataacc
     5461 gcaattaccc agtagac
//
LOCUS       DAZOZY010000244         5344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZOZY010000244 DAZOZY010000000
VERSION     DAZOZY010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.247"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHURQ_003868"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHURQ_003868"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR0825530.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHURQ_003869"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHURQ_003869"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR0825531.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHURQ_003870"
     CDS             complement(2698..3390)
                     /locus_tag="ACHURQ_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR0825532.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHURQ_003871"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHURQ_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR0825533.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1366 a         1380 c         1266 g         1332 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttt
//
LOCUS       DAZOZY010000245         5085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZOZY010000245 DAZOZY010000000
VERSION     DAZOZY010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.248"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..489
                     /locus_tag="ACHURQ_003872"
     CDS             <1..489
                     /locus_tag="ACHURQ_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0825534.1"
                     /translation="VNVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGH
                     HRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRR
                     RNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNAR
                     LS"
     gene            541..1476
                     /gene="ttcA"
                     /locus_tag="ACHURQ_003873"
     CDS             541..1476
                     /gene="ttcA"
                     /locus_tag="ACHURQ_003873"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR0825535.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="ACHURQ_003874"
     CDS             complement(1605..2978)
                     /gene="dbpA"
                     /locus_tag="ACHURQ_003874"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR0825536.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3008..3181)
                     /gene="ynaL"
                     /locus_tag="ACHURQ_003875"
     CDS             complement(3008..3181)
                     /gene="ynaL"
                     /locus_tag="ACHURQ_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR0825537.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="ACHURQ_003876"
     CDS             complement(3456..4439)
                     /gene="zntB"
                     /locus_tag="ACHURQ_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR0825538.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4453..4500)
                     /gene="ydaN"
                     /locus_tag="ACHURQ_003877"
     CDS             complement(4453..4500)
                     /gene="ydaN"
                     /locus_tag="ACHURQ_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR0825539.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4694..>5085
                     /gene="dgcM"
                     /locus_tag="ACHURQ_003878"
     CDS             4694..>5085
                     /gene="dgcM"
                     /locus_tag="ACHURQ_003878"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR0825540.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGF"
BASE COUNT         1382 a         1297 c         1211 g         1195 t
ORIGIN      
        1 gtgaatgtca gaagaaacct gacgatactt gatatgttcg gtcccccaaa aacaaatgcc
       61 gggatccgga cggtaacatt actgcagccg gctcttgaag cactgaagga gcaatacaaa
      121 ctgaccgggc atcatcgcaa aagcgaaatc actttttatc atcgggagta cggcagaact
      181 gaaaagcaaa aactgcattt tgttttcatg cccagggtgt gtaacgaaaa acagaaacct
      241 tattactcgg taagcagttt gggtgcaaga tggaatgcag cagtaaaacg tgctggtatt
      301 cgccgccgta atccgtacca tacgcgacat acttttgcct gctggctgtt gacggcagga
      361 gcgaacccgg catttatagc cagccagatg gggcatgaaa ctgcgcaaat ggtgtatgaa
      421 atttacggta tgtggattga tgacatgaac gacgaacaga tagccatgtt gaatgcgcgg
      481 ttatcgtagt tgcaaagttt gcccccaatt tgccccattt agtaccagag aactgaaata
      541 atgcaagaaa atcaacaaat tacaaagaaa gaacaataca acctgaacaa attacaaaaa
      601 cgtctgcgtc gtaacgtggg cgaagccatt gctgacttca atatgattga agaaggcgat
      661 cgcatcatgg tttgcctctc cgggggtaaa gacagctata ccatgctgga gattctgcgc
      721 aatttgcagc aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag
      781 caaccgggct tcccggaaca cgttctgccc gagtatcttg aaaagctggg cgttgagtac
      841 aagattgtcg aagagaacac ttacggtatc gtgaaagaga agattccaga gggcaaaacc
      901 acttgctcac tgtgttctcg ccttcgtcgc ggtatccttt atcgtaccgc aacggaactg
      961 ggggcgacga agatcgcgct gggtcaccat cgtgacgata tcctgcagac gctgttctta
     1021 aatatgttct acggcggcaa gatgaaaggt atgcctccga aactgatgag cgatgatggc
     1081 aaacatatcg ttattcgtcc gctggcctac tgccgcgaga aagatattca gcgatttgcc
     1141 gatgcaaaag cgttcccgat tattccgtgc aacctgtgcg gttcacagcc taacctgcaa
     1201 cgtcaggtga ttgctgacat gttgcgtgac tgggataaac gttatccagg gcgtatcgag
     1261 acgatgttca gcgcgatgca gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc
     1321 gatttcaaag gcatcaccca cggttctgaa gtggttaacg ggggtgatct ggcgtttgat
     1381 cgcgaagaga tcccactaca accggcgggc tggcagccag aagaagatga aaatcagttg
     1441 gatgagttac ggctgaatgt ggttgaagtg aaataaccag gatggcgccc gatgcgcaag
     1501 cgtatcgggc tactcttatg gaggccggat aagacgcggc cagcgtcgca tccggcaatc
     1561 ccgaataaga tgtttactct tgcacccggc aattcaacat ttcattattt taataaccgc
     1621 acccggctcg tttttccttt aatcttcccg ccctgtaact gtttccatgc tttatgagca
     1681 acagcctgac ggaccgcgac atagacatgc gccggatgca cggcgatttt gccaatatct
     1741 gcgccatcaa gcccgatatc tcctgtcagt gcacctaata catcacccgg gcgcattttg
     1801 gcttttttcc caccatcgat acacaacgtt gccatttctg cttccagcgg cacaatggaa
     1861 ctattagctg gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg
     1921 gcccgctgtg cttcttccgg agcacagaaa ctgatcgcca gaccgctatt tcctgcacga
     1981 gctgtacggc cgatgcgatg tacatgaact tcagggtccc acgccagctc aaagttcacc
     2041 accagctcaa gcgatttaat atccagacca cgcgcagcaa catcagtcgc aaccagtaca
     2101 cgggcgctac cgttagcaaa acgtaccagg gtctgatcgc gatcgcgttg ctccaaatcg
     2161 ccgtgtaatg acaatgcact ttgccctact tcattcagcg cgtcgcagac agcctggcaa
     2221 tcttttttgg tattgcaaaa caccacgcaa gaggatggct gatgcaagct taataaccgt
     2281 tgcaacagag gaattttgcc tttgctggat gtctcataaa attgttgttc aatgggtggc
     2341 aaagcatctg ttgagtcaat ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt
     2401 acggcaatgg cttccggcca ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca
     2461 aaacggatga catcatcaat ggcatcgcta aatcccatat ccagcatgcg gtcggcctca
     2521 tccatcacca aagtattcag cgcatccagt gataccgtgc ctttttgcaa gtgatccagc
     2581 aaacgacccg gcgttgccac gataatatgc ggcgcatgtt gcaacgaatc acgctgcata
     2641 ccgaacggtt gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccagc
     2701 cgacgcaatt cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc
     2761 tgggtttgaa atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt
     2821 ttgccgctgc cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgccggaagc
     2881 gcggcggcct gcaccggcgt catagttaaa taacccaact cattaaggtt cgtgagttgg
     2941 gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga
     3001 cgcaaagtta gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg
     3061 gttcttcatc gggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg
     3121 gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca
     3181 tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa
     3241 aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca
     3301 aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc
     3361 aagggagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg
     3421 tttttgtata cagacagccg gaaaaattgc ttttgttaca accatttact acggtgcaac
     3481 cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat
     3541 tgccacccgc cgccagggat cccaccaagg ttgacgccaa ataacccggt cagaaaggta
     3601 ctgggtaaaa agaccattgc catcaacgac attgtatagg tacgacgagc taaattttcc
     3661 tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt
     3721 tcgtcaaggc cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg
     3781 ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata
     3841 tagcgacgca tcacaattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt
     3901 tgctgatcaa ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat
     3961 tcgctggaat gatcggtcaa cgcatcgcac acatccacca gccaccctcc gcaatcggtc
     4021 ggacccgtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt
     4081 cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg
     4141 cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg
     4201 ctgactcggg gccgcgtgct ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc
     4261 ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag
     4321 ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc
     4381 agcatccagg caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca
     4441 gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat
     4501 ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag
     4561 caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
     4621 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa
     4681 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
     4741 ctggatcgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt
     4801 gatgcaagac ctcacgttta gtcagctacg aaccggtccc tattccgtct cctcacaaaa
     4861 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
     4921 tgttcagcgt aaagaagagg aaacagcatt aaactgtcgg cttgttttgc gaaagctgac
     4981 agaagcagaa ccggtgatta ttttcgaagg tatcgaagcg tcggcaacgc tgggtttaaa
     5041 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt tttta
//
LOCUS       DAZOZY010000246         5075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZOZY010000246 DAZOZY010000000
VERSION     DAZOZY010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.249"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..1135)
                     /locus_tag="ACHURQ_003879"
                     /pseudo
     CDS             complement(1..1135)
                     /locus_tag="ACHURQ_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1135..1317)
                     /gene="ymfR"
                     /locus_tag="ACHURQ_003880"
     CDS             complement(1135..1317)
                     /gene="ymfR"
                     /locus_tag="ACHURQ_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR0825541.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1329..3062)
                     /locus_tag="ACHURQ_003881"
     CDS             complement(1329..3062)
                     /locus_tag="ACHURQ_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR0825542.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3059..3553)
                     /locus_tag="ACHURQ_003882"
     CDS             complement(3059..3553)
                     /locus_tag="ACHURQ_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR0825543.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3675..4025)
                     /locus_tag="ACHURQ_003883"
     CDS             complement(3675..4025)
                     /locus_tag="ACHURQ_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0825544.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4068..4532)
                     /locus_tag="ACHURQ_003884"
     CDS             complement(4068..4532)
                     /locus_tag="ACHURQ_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0825545.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4529..5026)
                     /gene="rrrD"
                     /locus_tag="ACHURQ_003885"
     CDS             complement(4529..5026)
                     /gene="rrrD"
                     /locus_tag="ACHURQ_003885"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR0825546.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
BASE COUNT         1102 a         1485 c         1261 g         1227 t
ORIGIN      
        1 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tggaagagta aattccccag
       61 ttgatcccgg tggcgtaggc ttcaaaacgg gacttcatat ccccgcgcag taacgccccg
      121 gcgttaaatt tggcgtaata aacgccctgc ttactttttc gtaccagtcc ggtgttgatc
      181 cgctgttcga tgcgggtcag atacggcacc agtgaatagt tgataaatcc cagccccagc
      241 tcttcgatat tgttgaaggt ggcgcgatcg gtgttctgca ccatgtgcaa cggcacccgg
      301 aacagacgac agatttcttc cagctgaaac ttgcgggttt ccaggaactg actgtcctcg
      361 gcgttcagcg ccatcgactt ccagtccagc cccatttcca gaatcatcgg gcggtgagca
      421 ttgccaagcc ccgtgtgaca ctcctcaaaa tctttcttca gacgctcgta agcatccggc
      481 gtgagctttt gttccgtacg caacacaccg gatgtcaccg caccattact aaacaacctg
      541 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cacgcgcata cgcaatggga
      601 ttcagcccga caagtccatc cagcgtcagg gtgcgcacat gccagatttc attctgggtc
      661 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
      721 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccaccacttc tcccagtgcc
      781 tttaccttgt aggcgtaaaa attcccccgt agacacaggc agacaataac cagctcccag
      841 aattcctgcg gtgtcatgta gccattaggt tttgccgaaa ccaacttatg cagtcgttca
      901 tccaccgccc gtgttttcag ggtaccgctg attttgtaga gactgcaggg cagcatacca
      961 acagactctg ccagcaccct gacgcaagaa tagaccgccg tcaggcgcat ggcccgctgg
     1021 ctactgatcc gctttccggt ataggtgtcg tatgacagcc cgatagcatc agcagttctg
     1081 ctggcgtggt caccggcaca tcgctttttc gttgaaataa tcccgaaaag aacactattt
     1141 acctccgccg acagactgcc gtgtacggtc gagatatcgc gccaccagcc acgaccagaa
     1201 caggcacagc gccccggcaa caacaaaacc cgccggggga taaatcagcc aggcaccata
     1261 cgccagcaaa agcgcaccca gcacgcccac cagaggcgcg agaatcagca tgatcataat
     1321 tacctcagtt aaagcgagcg gatcccgtag gactcaatgt ggtcagacag cgtgtcttct
     1381 ttctcgtaca gcatggctct gccaaccgcc ataatcagcg caactgcacc atcgattttg
     1441 ttttccgcct gctctttgac gggtttcacc acatcatcgt tacccggcat gtttttgccg
     1501 accacgttgc cgatacacca ggtcatgatg ggattgccgt catgatgaaa acgtcccgat
     1561 tcaatcgctg cttccagctc tttcatcgga tcggacatat tggtgaagtt ctggacgata
     1621 gtgacgggat tcaggtcttc atcagcaagg tcatgtgaca acccggtcgc cccgaagggg
     1681 tcgatgggtg actcactgac cgggctgatt ttgttcgccg ctttggcctc ctcgaggatg
     1741 tagcgataat ccacctccgc accatcggta acggtcagaa cgcccatttc cacccatttc
     1801 tgaaagcgtt cggctgtccg tcgatcttca tttttctcga cgctgtacac cgtgtcatac
     1861 ggtacccaga aacgcggggc cacactgtag taatgcgttt taccgtcaat ctcgcgggta
     1921 taaagtcgcg ccatgctgtt catatccagc ttacgcgcca ggtcaaaggc cagaatgcac
     1981 ggctgcccct cgaactgctc aagggtcagt gatttatcct cgcagctctg ccagctcacc
     2041 aagttgaaat acgccgaacg cgccgacacc cagatattga ggtgttttgt tttaaagacg
     2101 tttgccagac gggcgttatt tttcgcacgc tgctgctgac ttaacaaaaa ttcgcgataa
     2161 accgacacgc caatatttgg attggctttt tccagcacct gcgggccggt ccagtcgtca
     2221 ccttcatcaa cggtatagat gatcccgaac agttcatcgt taggcaccga gccgttgagc
     2281 atctcgatga cttcccgccg cttgtcgtag cacggcccct caatgttgta cccggcggtg
     2341 gtgatggccc acatcagtgg ctgacgtcgc gcccccatcc cggtaagcat tgtggtataa
     2401 agcgcatcgg tggcatgctc gtgatattca tcaaccacgg cacagtgggg tgatgaacca
     2461 tcacctgggt tgccgatcag cggttcaaac cgcgcgccat cctccggacg gttcatgttt
     2521 gaggcgttaa cctcaatccc gaacgcttcc gtcagcatgg gtgtgcgttt acacatcagt
     2581 cgcgccgggc gaaagacttc ccacgcctgt ttctctgtcg tggcaccgga atacacttcc
     2641 gcgccaaact cgttatcaca ggcaaaacaa tacagggcaa caccggcaga gattgctgat
     2701 ttgccgttct tacgggggat ttcggtgtac acctcccgga agcggcgcaa ccgggtgcct
     2761 ttattgaccc agccaaacgc acagcagatc acaaatagct gccacggctc cagcgtgatg
     2821 ggcatccgtt tgaatgccca ctcccccttg gtgtgcggca acagctgaat aaatttcgcg
     2881 gcccgttcag ccaggtcctt gtcgaagcgg taacgaaacg acttactttt ttccgccatc
     2941 aggtcatcaa gatggcgctg gcaggcctga atcacaaact ggcaggcaac aatctttccg
     3001 cgcacgacat cccgggcata ctgatttgca gcatttacgt tggggtaaga tttccggctc
     3061 atgattcgat aattttcaga aacgggttag tggctttctt ctgccccgcc aggccaatca
     3121 gacgctggcg gctgctgggg tcgagtccga gcattgcccc cgtgctgctc atctcggact
     3181 cctgttcttt tttggcggtc agctccggat ttttgaccct gccgcccatt gcaccggtga
     3241 tggtgttgcc ctgtatggca atatttttca cggcacgtcg ccagaactca taggccacgc
     3301 accaccgctc aagtaccgcc aggtcagtca cgcacagcag gccctgaccg cagagttctt
     3361 tggttgtcag ttgccacatg atcgtggcga gagggagatc ttcttcagcg aaccactctg
     3421 gtggctcaac acctttgatg ggcgtaaaaa caggttcatc tttgttcagg gctcgcttgc
     3481 cggggtttcc ggccagcgcc ttgcgcgccg ttggcttggg gcgacgcccg gaacgccccg
     3541 ccgttccagc catatgcggc actcctggtt aaatttcatt tttcgcgggt ataaaaaaac
     3601 gatggggcgg gcagtccgga agacgtcagg tcacagggat ttgccccgcc cctccctaca
     3661 agtgagaata attatcaccg gattcgttcg cgcgctgttt tcgctttatg gcagggccag
     3721 cacagactct gcaggttgct gtctgcgtct gttccgccat gcgctttcgg gatgatgtgg
     3781 tcgacggttt tcgcctcgct caccacaccg acacgcagac acaactgaca cagaccttta
     3841 tcgcgcttca gaatacgggc acgaatcacc gtccattttg agccatagcc acgctggtgg
     3901 cggctcagtc cgcgctggtg ctgcacccag ccttcaccgc gatgtttatc gcagtaaccc
     3961 gaactgtctg tggttgtacc tgcacatcca cgcttacggc aggcacgtgg gattagtgat
     4021 ggcataaata cctcataccc tgcgaaatgt ttaccacgat aaaaaggcta cttaatgcac
     4081 tgagtgcgga tatactcctg tgccccttcc agttgcatct gcatcgtcat cagccgctct
     4141 ctgagggtga aataatcccg ttcagcggtg tctgccagtc gggggctggt tgcattatcc
     4201 acgccggagg cggtggtggc ttcacgcact gactgacaga ctgctttgat gtgcaaccga
     4261 cgacgaccag cggcaacatc agcgcgcaga gtttcatttt cagctttcgc atcagctaac
     4321 tccttcgtgt atttagcatc gagtgcatta acatcacgct gtctctgctg catgtcagta
     4381 atggttgcgt tcgccagctt cagttctctt gcgtttttgt cgcgctgctc tttgtaggta
     4441 atggcgttat cacggtaatg attaacagcc catgacaggc agacgatgat gcagataacc
     4501 agagcggaga taatcgcggt gactctgctc attgctgccc ccacaaacag acttcacgct
     4561 caatctcacg acgggtcatc agccctttcc attgcttacc gccagcatat gtccagcgac
     4621 gtagctggtc acatgcgcct ttgatatcgc cctggtttat tttgcgaaga agcgtcgatg
     4681 ttctgaaatt gccagcgccc acgttgtaaa cgaacgagta aagagcgccg cgcgttgttt
     4741 ccggtatatc gactttgatg tacgggttaa tttgtctggc gacagtggca aggtctttat
     4801 tcaggagggc tttgcattct gcttcggtat acgttttacc gagcatgatg tcttttccgg
     4861 tgtgtccgtg acatacagtc catacaccaa tgatatcttt gtatggtatg tagctgacac
     4921 cttccagacc atcgttacca cctgggccag tgattaacac agatgctata gcaacagccc
     4981 caccaccaat agcagctgca acagccttgc gtaatgatgg cgacattatt cacctctcgc
     5041 agccttacgc ttgtcttctc ttattttgaa ataca
//
LOCUS       DAZOZY010000247         5026 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZOZY010000247 DAZOZY010000000
VERSION     DAZOZY010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5026
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.250"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHURQ_003886"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHURQ_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="ACHURQ_003887"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHURQ_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHURQ_003888"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHURQ_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHURQ_003889"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHURQ_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR0825547.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHURQ_003890"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHURQ_003890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHURQ_003891"
     CDS             complement(2927..3859)
                     /locus_tag="ACHURQ_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR0825548.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHURQ_003892"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHURQ_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR0825549.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1226 a         1166 c         1174 g         1460 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggacgtaat gatgtaaaaa tactga
//
LOCUS       DAZOZY010000248         4970 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZOZY010000248 DAZOZY010000000
VERSION     DAZOZY010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.251"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            236..1210
                     /gene="hemB"
                     /locus_tag="ACHURQ_003893"
     CDS             236..1210
                     /gene="hemB"
                     /locus_tag="ACHURQ_003893"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR0825550.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1316..2167)
                     /gene="tauD"
                     /locus_tag="ACHURQ_003894"
     CDS             complement(1316..2167)
                     /gene="tauD"
                     /locus_tag="ACHURQ_003894"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR0825551.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2164..2991)
                     /gene="tauC"
                     /locus_tag="ACHURQ_003895"
     CDS             complement(2164..2991)
                     /gene="tauC"
                     /locus_tag="ACHURQ_003895"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR0825552.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2988..3755)
                     /gene="tauB"
                     /locus_tag="ACHURQ_003896"
     CDS             complement(2988..3755)
                     /gene="tauB"
                     /locus_tag="ACHURQ_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR0825553.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3768..4730)
                     /gene="tauA"
                     /locus_tag="ACHURQ_003897"
     CDS             complement(3768..4730)
                     /gene="tauA"
                     /locus_tag="ACHURQ_003897"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0825554.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1175 a         1421 c         1247 g         1127 t
ORIGIN      
        1 gaattattca atttatagca gcacaaccaa acgccaaaaa aaataataac caagagctat
       61 aaataaagat taaaaatatt ttcagtgaat atgaaattat tgctatttct attacaaaga
      121 tgtcctggtc tctctttcat tctcttcttg tcaacatcgc gacaactttc gtaaaacact
      181 cctgccccgc ttcaggtata ctgtgcacct taattccaca aacatcaggc agaccatgac
      241 agacttaatc caacgccctc gtcgcctacg caaatctcct gcgctgcgcg ctatgtttga
      301 agagacaaca cttagcctta acgacctggt gttgccgatc tttgttgaag aagaaattga
      361 cgactacaaa gccgttgaag ccatgccagg cgtgatgcgc attccagaga aacatctggc
      421 acgcgaaatt gaacgcatcg ccaacgccgg tatccgttcc gtaatgactt ttggcatctc
      481 tcaccatacc gatgaaaccg gcagcgacgc ctggcgggaa gatggactgg tggcgcgtat
      541 gtcgcgcatc tgcaagcaga ctgtgccaga aatgatcgtc atgtcagaca cttgtttctg
      601 tgaatacact tctcacggtc actgcggtgt gctgtgcgag catggcgtcg acaacgacgc
      661 gactctggaa aatttaggca agcaagccgt ggttgcagct gctgcaggcg cagacttcat
      721 cgccccttcc gccgcgatgg acggccaggt acaggcgatt cgtcaggcgc tggacgctgc
      781 aggctttaaa gatacggcga ttatgtcgta ttcgaccaag ttcgcctctt ccttttatgg
      841 cccgttccgt gaagctgccg gaagcgcatt aaaaggcgac cgcaaaagct atcagatgaa
      901 cccaatgaac cgtcgcgagg cgattcgtga atcactgctg gatgaagccc agggcgcaga
      961 ctgcctgatg gttaaacctg ctggagcgta cctcgacatc gtgcgtgagc tgcgtgaacg
     1021 tactgaattg ccgattggcg cgtatcaggt gagcggtgag tacgcgatga ttaagttcgc
     1081 cgcgctggcg ggtgctatag atgaagagaa agtcgtgctc gaaagcttag gttcaattaa
     1141 gcgtgcgggt gcggatctga ttttcagcta ctttgcgctg gatttggctg agaagaagat
     1201 tctgcgttaa tttttttcct ctcagaaagg gggagttaac ctgttgaaag tacggtgaac
     1261 gccccctcac cctaaccctc tccccaaggg ggcgagggaa ccgtccgcac tcgtatcacc
     1321 ccgcccgata aaacggttta tccccaagga tcgtcgcccg atgcataatc cgccgctgtg
     1381 gcaggtaatc ggcattggca tagtgctgag tcacgcggtt atcccaaatc gcaatatcat
     1441 tcggttgcca gcgccaacgc acctgaaact ccggtttagt gatatgggcg aataaaaagc
     1501 ctaacaaggc ttcgctctct ttctcgctga catcgacaat tcgcgtagta aagccttcat
     1561 tcacaaacag cgcctgttta ccgctcaccg gatgcgttcg caccaccgga tgtagcaacg
     1621 gaggattttt cgcgaccgcc tcgcgccagc gttgatgttc ctcctcggtt ttgcggtatt
     1681 tgtattccgg gaacgattta cggaaatcat gctccgcacg cagcccgctc agcagctggc
     1741 ggaagggaac agagagcgcc tcataggccg caataccgct ggtccagagc gtatcaccgc
     1801 cggtcgaagg taactcttta gctgccagaa tcgcccctgc gggtggcgtt tcaataaatg
     1861 tcacatcggt atgccagttg tcgttatccg gcggattatc gttatgggta tccagcacga
     1921 tgatctcgtc aaccccttcg gcatgagggt aaacagggtg aatatgcagt tcgccaaaac
     1981 gctgggccag cgcgcgttgc tgctgcggcg taatagcttg atcgcgcaga aataccacct
     2041 gatagcgcag caccgcatgg taaagctgtt caaactgatt atcgcttaac gggcgcgtca
     2101 ggtcggcacc cgaaatttgt gcgccgatat acggccccag cggggtaata ctcagacgtt
     2161 cactcattgt acttctccat gccagggcgt cagacggcgc tgtaacgcgc gcagacccag
     2221 ttctaaaaga aaggcgataa tcgcaatcac cgcgatcccc gccagtacca cgtcagtcgc
     2281 gagaaattca cccgctgact gaaccataaa tcctaaaccg cgtgtcgcgg caatcagctc
     2341 cgccgctacc agcgtagacc agcccacacc cagaccaatg cgtaatccgg tgaggatttc
     2401 cggcagcgcg ccgggcaaaa tgacaaacca cagcacctgc gcacggctgg cacccagcga
     2461 ctgggcggca cgaatgcgaa cctgctgcgc gcttttcacc cccgccagca ccgacatcgc
     2521 caccggtgca aaaatcgcta aatagatcaa taagatcttc gaggtttcac caataccaaa
     2581 ccagatcacc atcagcggca aataagccag cggcggcacc ggacgataaa gttcgattac
     2641 cggatccaga atgccgcgca ccgtcgggct tagccccatg gcgatcccga ccggaatacc
     2701 gatcaccacc gccgcgagta gcgccagcac aatgcgcgtc aggctggctg ccagatgctg
     2761 ccacagcgtg gcgtccataa atccctgcgg tccggcaatg gtgagcagtt ttgccagtac
     2821 ttgttgcggc ggcggcaaaa acagcgggct aatcagttgc agcgccgcca ccgtccacca
     2881 tacagtgagt aaaaccgcta acgtgccaat gcttaaggtc acctgacgcg agagcggcca
     2941 gcgccatttc agccgccgcg aatgcagttt ttcattaatg agcacactca tgagaacgcc
     3001 tcccgttgct caaatacgcg gcttaaaaca tattcgcgca tggcgatgaa ttgtggatcg
     3061 gacttgatgc tgcggctcga ctctcccgca acaaagcggc gagcaaagtt gagcggcagc
     3121 cgctccagca cacggccagg gccggatgaa agcagaacca gttcagtcgc cataaacacc
     3181 gcttcttcta tatcgtgggt aatcaacagc acctgcttgc ccgtctcctg ccagagtttc
     3241 agcagcagag tttgcatctg gtcgcgggtg aaggcgtcca gcgcaccaaa cggttcgtcg
     3301 agtaataaca gctggggatt cgccgccagc gcgcgggcaa tccccacccg ctgacgttga
     3361 ccaccggaaa gctgccagat atagcgtttt tctgcgcctt ccagccccac ttttttcagc
     3421 atctggtgcg cgatttccag tcgctgcatt ttctctatac ctgccaattg caggccgaac
     3481 gccacgttgt cctgtacatt gcgccacggt agtagccctt cattctgaaa aaccaccccg
     3541 cgctctgctc ccggtccctc aatacgctta cccgccagtt gaatgctgcc atgctgataa
     3601 ggcacaaaac cggcaatcag attcagcagg gtggttttac cgcagccgga cggccccagc
     3661 accaccagta gctcgccgct ttccagcgtc aggttgatat cttccagtgc cggtttgccg
     3721 ccataatcgg catagagatg agagatttgc agcatctgcg cctcctttta ttgcacgaag
     3781 cgcgaggtaa cgtactggct gtaatcattc gctacagccg ggaccttgcc ctgctctttc
     3841 aaaaactgcg cggtgtcgat gattgctttg ttcaccggtc cggtcagttc tgccgtttgt
     3901 tgctgcggcg tcagataggt attccccttc accagccccg gaacgtcacc ttccggcacg
     3961 ccgcttaaac gcgccagttt gctgatgttt tccggctgtt tcagccacgc gtctgggtta
     4021 gcaatgtacg gttgctgagc atcgatggcg cttttagcga acgctttcac gacctcagga
     4081 tgtttctcgg caaaatcttt gcgcaccacc cagacgtcca gcgttggcgc accccactgc
     4141 ccgacctgtt cagaatcggt caacaccttg ccgtcttttt ccagggcgtt aaccgccggt
     4201 gcccagacat aagcaccatc aatatcaccc cgctgccatg cggcgataat cgcgggcggc
     4261 tgcaggttca caatctccac ttgccctggt ttaatccccc agtgtttcag cgccgccagc
     4321 aggctgtagt gggtggtgga gataaacggt acggcaatgc gtttgccaat caaatcttcg
     4381 ggtttgctga tagttttctt taccaccagc gcttcggagt tacccagttt tgacgccagc
     4441 aagaagactt caatcggcac ctgttggctg gctgcaaccg ctaatgggct ggaaccgagg
     4501 ttgccgattt gcacgtcgcc agaagccagc gcccgcacga tgctggctcc gctgtcaaac
     4561 ttacgccagt ccacgctcgc cccgctctct ttagcaaagg tgttgtcggc ctgagccact
     4621 ttcgccggtt cggctgaggt ttgatacgcc acggtgacgt tcaccgcctg tgcctgaaaa
     4681 gcgatgaatg ccagtgcggc aagaagtgtg ttacgcgatg aaattgccat gattgtctgc
     4741 tcccctgtct tgttatggga gcagtattca ggaataaaaa cattcattaa aagaattagt
     4801 cgttatcgca cagatgattt tattcttagc aaaaaaacgg caaaggaaag attaaatatg
     4861 caattactcc gttctaacaa ttgggaaaat tactacactt cattttagtg atgcataaac
     4921 aaccacatta cgagatttat tacttcattg tttctatcaa tacatataat
//
LOCUS       DAZOZY010000249         4948 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZOZY010000249 DAZOZY010000000
VERSION     DAZOZY010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.252"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            8..559
                     /locus_tag="ACHURQ_003898"
     CDS             8..559
                     /locus_tag="ACHURQ_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0825555.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            619..3123
                     /locus_tag="ACHURQ_003899"
     CDS             619..3123
                     /locus_tag="ACHURQ_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0825556.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3139..3888
                     /locus_tag="ACHURQ_003900"
     CDS             3139..3888
                     /locus_tag="ACHURQ_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0825557.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3890..>4948
                     /locus_tag="ACHURQ_003901"
     CDS             3890..>4948
                     /locus_tag="ACHURQ_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825558.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT         1532 a         1012 c         1020 g         1384 t
ORIGIN      
        1 gaatattatg tctgctttta aaaagtcact tcttgttgct ggcgtagcaa tgattctttc
       61 caataacgta tttgctgacg aaggtcacgg cattgttaaa tttaaaggtg aagttatctc
      121 tgctccatgt tcaattaaac caggtgacga agacttaact gtaaatctgg gtgaagtagc
      181 agataccgtt ctgaaaagcg accagaaatc tttggctgag cctttcacta ttcacctgca
      241 agactgcatg ttgagccagg gtggaaccac ttattcaaaa gcgaaggtta ctttcaccac
      301 agcgaatact atgacgggtc agactgacct gctgaaaaac accaaagaaa ctgaaatcgg
      361 tggtgccaca ggcgttggcg tacgtattct ggatagccag agcggcgaag tgactttagg
      421 tactccggta gtaattacat tcaacaatac taacagctat caggaactga acttcaaagc
      481 tcgtatggaa tccccgggca aagatgctac cccgggcaac gtttacgctc aggctgacta
      541 taagattgct tacgagtaat cacattaggc cgggttcgcc cggccacatc attaacggat
      601 taatgataag tggaacagat gtataaaaaa ttaaaattaa ccacaataag cgaattgatt
      661 aaaaatattt attgttcatt atccgttctc atcattggtt gtgcgtcagc ttatgccgtt
      721 gaattcaaca aagatttaat cgaagccgaa gatcgtgaaa acgttaacct ttcccaattt
      781 gaaactgatg gccaattacc cgtaggtaaa tactcattaa acgctcttat taataataaa
      841 aggacgccaa tccatctgga cctgcaatgg gtattaattg ataaccaaac tgcagtttgt
      901 ctgactccag agcaattaac attattaggc tttactgatg agattattga agaggctcag
      961 caaaacctga tcgatggttg ttaccctatc gaaaaagaaa aacaaattac aacttatctc
     1021 gataaaggga aaatgcaatt atccatatct gcacctcagg catggttaaa atacaaagat
     1081 gcaaactgga cgcctcctga actttgggat catggtattg ctggggcatt tcttgactac
     1141 aatttatatg cctctcatta tgcaccacat cagggcgata attcacaaaa tataagttcc
     1201 tatgggcagg ctggggttaa tcttggggcc tggcgcctgc gtactgatta ccagtacgat
     1261 cagtcattta acaatggcaa aagccaggcg aacaacctgg attttccgcg tatttatttg
     1321 tttcgcccaa tcccagcaat taatgcaaaa ctaactatag gtcaatacga tactgaatcc
     1381 tctattttcg actctttcca tttttctggc gtttcgttga aaagcgatga gaatatgttg
     1441 ccgcctgatc tacgtggtta cgcaccgcaa atcacgggtg ttgcacaaac gaatgcaaag
     1501 gtcactgtct cacagaacaa ccgtattatt tatcaagaaa atgttcctcc tggcccattt
     1561 gctattacta atttattcaa tacattacag gggcaactgg acgtcaaggt tgaagaagag
     1621 gacgggcaag tcacgcaatg gcaagttgca tctaatagta ttccttatct gacgcgtaaa
     1681 gggcagattc gctacaccac tgctatgggt aaaccgacca gcgttggtgg tgattcctta
     1741 caacaaccct tcttctggac tggtgaattc tcatggggtt ggctgaacaa tgtatccctg
     1801 tatggtggtt cagttttaac aaaccgtgat tatcaatctc tggctgccgg cgttggcttt
     1861 aaccttaact cattgggttc attatctttt gatgtcacac gatctgatgc tcagttgcat
     1921 aatcaggata aagaaacggg ttatagctac cgcgctaact attcaaaacg ttttgaatct
     1981 accggtagcc agctcacttt cgctggttac cgtttctctg ataaaaactt tgtgacaatg
     2041 aatgaatata tcaatgacac taaccattac atgaattatc agaatgaaaa agagagttat
     2101 attgtcacgt ttaaccagta tcttgaatca ttaaggttaa atacatacgt aagtttggct
     2161 cgtaatactt actgggacgc cagcagtaat gtgaattatt cattatcact tagccgcgat
     2221 tttgatatcg ggccattaaa aaacgtctcc gcttctctaa catttagccg aataaactgg
     2281 gaagaagaca accaggatca actgtaccta aatatttcga ttccctgggg aaccagtaga
     2341 acattgagct atggtatgca acgaaatcag gataacaaga tttcgcatac tgcttcgtgg
     2401 tatgactctt ccgatcgaaa taattcctgg agcgtttctg cttcaggcga caatgatgaa
     2461 ttcaaagata tgaaagcgtc actacgcgcc agttatcagc ataataccga gaacggtcgt
     2521 ctctatctct ccgggacatc acagcgagac agttattatt ctctgaatgc cagttggaat
     2581 ggttcattca cggcgactcg tcacggagcc gctttccatg actatagtgg tagtgctgac
     2641 tcgcgtttta tgatcgacgc agatggtgca gaagatattc cgttgaacaa taaacgcgcg
     2701 gtaactaatc ggtatggcat cggagttatt ccatcagtca gcagttacat aacaacatca
     2761 ttaagcgttg atacccgaaa tcttccagaa aatgtggata tcgaaaactc ggttatcacc
     2821 accaccttaa ccgaaggtgc tattggctac gccaaacttg atacccgcaa gggctatcaa
     2881 atcatggggg ttattcgcct ggcagatggt agtcacccac cactggggat tagcgtaaaa
     2941 gataaaacca gccataaaga attaggactg gttgctgatg gcggctttgt atacctcaac
     3001 ggcattcagg atgacagcaa acttacttta cgctggggtg ataaatcctg ttttattcaa
     3061 ccacccaata gcagcaactt aaccaccgga acggttattt taccgtgtat tagccaaaat
     3121 taattcagga tgaaactaat gcgctactta aacacaaaga atctcattgc agcaggagta
     3181 ttactcgcct gcatgagcag cattgcctgg ggtgccatca tcccggaccg tacccgtatc
     3241 ataatgaatg agtcagataa aggggaagca ttaaaactta ctaatcaaag caaaaatcta
     3301 ccctatctgg cgcaaacatg gatagaagat acgaaaggca ataaatcacg tgactttatt
     3361 gtcacggttc cccctatggt acgtttaaat cccagtgagc aaattcaaat aagaatgata
     3421 agtcaggaaa aaattgctca actgcctaat gacagagaaa cgctcttcta ttttaacgtg
     3481 cgagaaatac cgccaaaaac agataaaaaa aatgtgatgc aagtcactat gcagcacgca
     3541 ttgaaactat tctggcgacc aaaagctatt gaattagaag atgatggggt aatgtcttac
     3601 gaaaaagttg aaatcatccg tagaaatgat ggttcgatcc gctttaacaa taagatgcct
     3661 tatcacgtta cgctgggtta tatcggaaca aacggaatca caatgttgcc acaaacgcaa
     3721 agtttaatgg taacccctta tagccatgca gatacgcagt ttaaaaatgt tccgtcagct
     3781 tttcaggttg gttatatcaa tgattttggt ggattaagtt tttatgaaat aaactgcccg
     3841 acggttaata actcttgtaa tgtttccgta gccaaacgag ataaataata tgcgctactt
     3901 attaattgta attatattta ttataggttt tagtgcatta cctgtatggg caatggactg
     3961 ctatgctgaa catgaagggg gaaataccgt agtcattggt tacgtaccaa gaatttctat
     4021 ccccagtaat ggtaaaaaag gtgataagat ctggcaaagc agtgagtatt ttatgaatgt
     4081 cttctgtaat aatgcattac ctgccccatc acctggagaa gaatacccat cagcctggac
     4141 taatataatg atgtttttac caggcggtca ggacttttat aatcagaact cctatatttt
     4201 tggcgtaaca tacaatggag ttgattatga ttcgactgcc cctaatgcta tcgcagcacc
     4261 agaatgcatt gatataaaag gagcgggtac ctttaataat cactataaaa accccgctgt
     4321 ctgtagtgga ggtcctgagc ctcaattatc agtaaccttt ccagctcgcg tacaactcta
     4381 tattaaatta gcaaaaaatg ccaatagagt taataaaaac ctggtgttac ctgatgaata
     4441 tatcgctcta gagtttaaag gtatgagtgg cgcaggagct atagaagtag acaaaaattt
     4501 gaccttcagg attcgtggat taaataatat tcatgtcctt gactgctttg ttaatgtcgc
     4561 actggaacca gctgatggcg ttgttgactt tggtaaaata aattcccgaa caattaaaaa
     4621 taccagcgtt agtgagacgt ttagtgtagt catgacaaaa gatcctggtg cggcctgtac
     4681 tgagcaattc aatattttag ggagcttttt cactacggat attttgagtg attatagcca
     4741 tctggatatg ggtaatggac tgctattgaa gatatttcat aaagatggaa cagcaacgga
     4801 atttaaccgc ttctcacaat ttgcttcttt ttcatcgtct agtgcacctt cggtcaccgc
     4861 accattcagg gcagaactta gtgcgaaccc ggcagagact gttgttgagg gaccgtttag
     4921 taaagacgta atcctcaaaa tcacctat
//
LOCUS       DAZOZY010000250         4921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZOZY010000250 DAZOZY010000000
VERSION     DAZOZY010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.253"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            175..837
                     /locus_tag="ACHURQ_003902"
     CDS             175..837
                     /locus_tag="ACHURQ_003902"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR0825559.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1201..2087
                     /locus_tag="ACHURQ_003903"
                     /pseudo
     CDS             1201..2087
                     /locus_tag="ACHURQ_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2087..2377
                     /locus_tag="ACHURQ_003904"
     CDS             2087..2377
                     /locus_tag="ACHURQ_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825560.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2698..2988
                     /locus_tag="ACHURQ_003905"
     CDS             2698..2988
                     /locus_tag="ACHURQ_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825561.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2988..3407
                     /locus_tag="ACHURQ_003906"
     CDS             2988..3407
                     /locus_tag="ACHURQ_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825562.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            3575..3898
                     /locus_tag="ACHURQ_003907"
     CDS             3575..3898
                     /locus_tag="ACHURQ_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825563.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            4328..>4921
                     /locus_tag="ACHURQ_003908"
     CDS             4328..>4921
                     /locus_tag="ACHURQ_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825564.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1623 a          898 c         1002 g         1398 t
ORIGIN      
        1 tctgaaaagt tactgtacag aatatataca gatatcttta aagtatctgt aaaaactttt
       61 aagataaaag atagatatcc cagaaaggat atccgtttag aaaacctgtt aagtatctct
      121 taaaagtaga gtaaaccttg tcaagagtga ttgcaataaa aatttttatt tcttatgtca
      181 attgagcata ttttaatcgg gaagcatcat gggggttctg attactggag aactccattt
      241 ttgttattta agaacttaca ccgtgaattt attttcagtc tagatggtgc tgcaactgag
      301 catgacacgc tgttaccacg attcaccgat gatataagcc gtcaatcatg ggttagtgaa
      361 aaggtcttct gtaacccgcc gtacagcgat attccgtcgt tcctcttaaa agcgtctgaa
      421 gcggatttag tagctttcct gataccgcat agagccaaca ccagctactg gcttaggcac
      481 atttacagca ataaccactg tcatgaaatc cgtattcttc atcgtgcagt taaatacctt
      541 ccaccagcag ggcataacag gcttactatt aggtcaccgt ttccaagtgc agtcgttgtt
      601 tttaaaaaag aaccacgtaa gcatgaaatt acacagatgg tttgctgtgc tgatacattg
      661 ctaccgttaa ccatcattaa ccgtggtgga ctaagaggaa gacccaccat ttacccacct
      721 gagacgctcg acagcttcat aaaactatac aggcaaggaa aacccattaa gtacattgca
      781 gatgctctca gaatgcctct gagcacgtca tacaggattg ctcaaaggtt gagctaaaat
      841 tctcataaat atggtcattt acaagacagg tgattcagat gttaagaagt atccctgtga
      901 acaataaatg accaatcagt aatcattctg tgaaataatt gttgcaattt ataatcacat
      961 ctgttatagt ttctatatgg gctgataaaa aagttcatgt cttgaagtgg taacacttgg
     1021 ggaggtgtca gatggttggc gtatattaac ctctcattca gggtatacct cagcgtttta
     1081 atcatcgtac tgtttcaggc atggacttct ttatcagtcc atcaatcccc cagatatttt
     1141 aatcacacag gctaatcggt tttatccggt tggccttttc acgtatgagg taatttatga
     1201 atgtctaaat ataacccaaa tcagttttat aaatttgatg tgcgaatctc tcaactttca
     1261 gaagaaatta acgcagcagc aagattgatt tatatggtca tggtccatag ctgtgacttc
     1321 tatgaagaga aatacaaatc atattcacca agtattgacc atctcgctta tatggctggt
     1381 gtatcaaaga gaacagcaat cagagccatt gagacacttg agaaacacgg tttaattcag
     1441 gtgcaaaaga atcaggggcg tcaaagcact tatcgtgtat tcacactgga tgataagccg
     1501 gaattacttg taagacctac atcacaggaa gttgcagcct cagtaagaca aaatgacacc
     1561 actaccagtg acaaaatgtc atgggggtag tgacaaaatg tcacccgtgt tagagagata
     1621 agttattaga taaaacatta gataataata ttacagataa gaataaagag actggactta
     1681 atgatgatat taaagaagat gactttcttt ctttggatga attaaatgat tttctctcta
     1741 tgaatgaatc tgaccttgaa gtaagctcta aaaagacttc taaacaagac agagaaatca
     1801 ctgatgtgag tgttgatagt gaccataaaa acacacagtg ccccgccact gatgatgagt
     1861 tggacccctt cgatgatgaa gaggatgaac gtgaaaaagc cattaagagg gcagagaaga
     1921 aaaagagatt cgagcttgca agacaaaaga acaaagtatc gggtttagac ccattcgatg
     1981 acagtgaaga tgattattat ctttcaccga aaaagaaaca aactcaggat gacaagaagc
     2041 aaaaaattaa accgaagatg tattttgccg gtggaggaat taagtaatga aagaatatca
     2101 cggtgaaaag agatacaaag attacctgtt aaagcgttac agcatttctc gtgaaggtca
     2161 tcttatgaag gatactcacg gggaagttta ccggattaga ccaaagaaag aagggagggg
     2221 ttatttcttt tacgatggtg tgacagattt aaagattgac gctttgaggt ttgcagtgct
     2281 gtatcatttt gatatctgga attcaatcca ccagctacga ttaaaagacg gtgaccccag
     2341 caacttaaag gccaccaata ttatcacaaa acgttagttt gttttaccat catcaatcgt
     2401 aattgtgatt tcatatttga aaagcttaat gctgaaattg aatcgtgtac ttctggcgat
     2461 aatcattttt aaaagctgat acacaagaag caccagaaac acaaagcaac ctaagcttgc
     2521 gaatctgtga gattcagcaa ttgtttcaag aaagcccatt aattcccctt atttatcaaa
     2581 gggaatgacc tgtatcagcc ccctacaacc atattaacat gtaaatgagt tgttgacaat
     2641 atgttataaa tatgttaaag ttagttataa gtcaaagatt ttaagaagga aaatttaatg
     2701 agacacacag ttacacgcct tttgccagag gcatattcca gactggcagc aatcaaagac
     2761 aaatacccag ccatgagcca cacagcactt gttggtgaga ttctgaagga acacacagcc
     2821 cttacaccgt ttgacgctat caagggcgtt gcagagggtt ctaaggccat cagaatcaca
     2881 acgggccaac tggaactact caaagcacag tccgcagtgg caggttgctc aatgattcag
     2941 gtgttgagtg ccaaggttca tcaactggcg gataagctgg gggtttaatg ctatgcacat
     3001 ttgattatca tgcgttttat cgccatagag gtcaccttgt ccgtgagaat aacggaacat
     3061 ggaaagattt aactcgtaat gctgaaccct tcacacacca gcaattaaaa tcccgcttct
     3121 ccagatggaa gaatgcacca gcagatactg gtgattttga gattatccat accttcgatt
     3181 atgcctcaga agatgacctt cacgatgcaa ttaagctgat tgaagacttc aatacctcag
     3241 aagatgtgga tggtgacaaa tataaatccc gtggtaaccg ctataaccgt gatgatatca
     3301 ctcaggccat aaatgctggt caatctcagc gatacattac agaaaaatat ggtatttcac
     3361 ggagacagta tttctacgtt aagtcattga ttaataaaga caattaaaaa taaccatcta
     3421 agaaacggta gaatgcactt tacaggcaga gttgcacctt aatttgataa attaccttac
     3481 cagccagtgc aactccctct gtaacccgca tggttactgg cctcagaatg gctattttaa
     3541 gaggtctgag gtgtgtttca taggagattt aaaaatgaat aattacccaa tgcaaattat
     3601 ggttgataac gacactgcta tgatggtgca aagcttcatt gatgcaggta tcgcaattaa
     3661 ttttgataaa ttactaaagc tgatggctga taatgcagaa aacatctctg actttattca
     3721 gtcggtggag tttaacgagc ctcgaatgat gctaccaatt acagacagca acatgaaaag
     3781 attggttatc gagcaaacga acatatacag tatttcccca gagcaatttt tgaaaggtgc
     3841 tgtgacaatc ctgtacagtg ataatattct ggtgacggat tctgtgaggg ttcactaatg
     3901 ggtttaataa ttgcttggac gttgcttggc ttatttgttg gttgcattat taacaatgcg
     3961 gtaaataaga attgatgtta tcaacctgtt aaaaagctgt tgacaaataa ccttaagcat
     4021 ggcataattt taattatggg agagcaattc tctcttattt ttctttcaat aatccgatgg
     4081 atggatattc tgagttggaa aggtgcaatg cttttcgtca tggcctttca ggttagtctg
     4141 agaggctatc tcaaaaagta ctcttgttca ttcattattg aatcctttgc ccttcacaga
     4201 aatgtttagg gctttttcag tttttaaaag cacacttggc aggttcttgt tcagtcagac
     4261 tcttcacctc tgattgctga gtgtgcttct ataaattgaa ataacaaata aactaagggg
     4321 tgaacgaatg attagaaatg aacatgaaat gcttatcgaa cacagaccaa gtgcacttga
     4381 tattcacgaa ttaactttgc gtgaatcttt tgatagagct tggcgggaat cacacgaagt
     4441 aaataaaggt ccatatattc caccagaacc actcgagatt ccaagagttg aaattgattt
     4501 ttctatgaac gaacggtgtg aacttgattt gaagttgaag ctacaacgcc gatatttcca
     4561 agctattaaa gactctcaag aagcaatgag aagtgtttac gagaaaattc agtccaagct
     4621 tgaagacgaa attgacgaaa tggttatttc gatggagctt caaaccaatt cttatgcttg
     4681 tttctctcga ctagaccgtg acggttgggg gtatgaccct gtagagattg gtaaagtgat
     4741 tgcagattta ccagaaggtc aaagagttgt tcgtacagaa agagctgtca gagcaccaca
     4801 tcgactgtct ctaattgttg ctgataaatc acaagctgag cttgaagaaa ttgccaaaga
     4861 gaaactgatg aaagcaagag aaaatgagat taatttgctg aaatcaagac tggctaagct
     4921 g
//
LOCUS       DAZOZY010000251         4912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZOZY010000251 DAZOZY010000000
VERSION     DAZOZY010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.254"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHURQ_003909"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="ACHURQ_003909"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR0825565.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHURQ_003910"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="ACHURQ_003910"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR0825566.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1114 a         1363 c         1253 g         1182 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttactta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atgttttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaaacga
     4801 gcaggaaaaa gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa cagaagacaa
     4861 aacatgtaat ttttggcact tatagaatat ttaaaaacat aacactaaaa at
//
LOCUS       DAZOZY010000252         4898 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZOZY010000252 DAZOZY010000000
VERSION     DAZOZY010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.255"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(53..1336)
                     /locus_tag="ACHURQ_003911"
     CDS             complement(53..1336)
                     /locus_tag="ACHURQ_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFR0825567.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKQPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1449..3383)
                     /gene="yeaG"
                     /locus_tag="ACHURQ_003912"
     CDS             complement(1449..3383)
                     /gene="yeaG"
                     /locus_tag="ACHURQ_003912"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFR0825568.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3463..>4898
                     /locus_tag="ACHURQ_003913"
     CDS             3463..>4898
                     /locus_tag="ACHURQ_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0825569.1"
                     /translation="MLRPICYERVSSIQQVDMGKGLDDQRSSLTAYLDKNADKFTDER
                     IFITDAGLSAFKNANISPESQLGIFLQDVGNRKYGVGDALVVLSLDRLSRRSSWAEST
                     IQYIVNSGIEIHDISTNLVLRVDDPMSKIHMELIQHRSHNESLMKSVRAKLAWDNKIT
                     RAVQNGEVISNRMPNWLRNVDNKYQVIPEQAELIVKCFMWYREGLSTGEIVKRIGRKW
                     QMVTVSRLIRDRRLLGEHERYNGEVVPNVYPKIIDEELFLTANRMMDRVMLEKKKPAE
                     DLLLEPEVVREIFKLYETGLGSGAIVKRLPKGWSTVNVLRVLRDKKVVTMKIIDNLTF
                     ERANEKLSRNGIANRIRKDITIAQDDYITNLFPKMLKCGHCGGNIAIHYNHVRTKYVI
                     CRNREEKKICDAKSIQYMRIEKNILQLVKNVDFNKLMMEGKSDSDSLLASLREELSTL
                     QKEAETYQVKINERKAAGKKASLPLMTG"
BASE COUNT         1254 a         1118 c         1201 g         1325 t
ORIGIN      
        1 aatttacagt aagctaaaaa tcagccaggc gataatgacc tggctgatag ttttagtctt
       61 tagctgttgc attttgttta tgaaacagtt cacggaacac cggataaata tcatcctggt
      121 cgcggatgtg ctgcatcgca aagttgtcga aagtagattg cagatgctca tattctcgcc
      181 acaatgtctg atgtgcacga cgggtaattt cgatatagct gtaataacga acaacaggta
      241 ataatttttt cgccaggatt tcatggcaaa gcggagagtc atcggcccag ttatcgccgt
      301 ccgatgcttg tgcagcgtaa atattccact gtgccgggtt ataacgctct ttcactacct
      361 catccatcag tttcagggcg ctggaaacaa tggtgccgcc tgtttcctgc gagtagaaaa
      421 actcatgttc atcgacttct ttcgcctggg tatgatggcg gatgtatacg acttccacgt
      481 tcttatacgt tctgctgagg aacagataca gcagaatata aaaacgctta gccatatctt
      541 tagtggattg atccattgaa ccggaaacgt ccatcaggca aaacatcact gcctggctgg
      601 agggatcggg ctgcttctcg tagttcttgt aacgtaaatc gaaggtgtca ataaaaggga
      661 cgcgttcaat tttggcacgt aattctgcaa tttctttgcg cagacgttcc tcttccagca
      721 gttgcgcagg ttcactgttg ctgatgatgg ccaaattctc ttccagcgca tgaagttccc
      781 gtcgcttgcc cgccgtcatg gctgtgcgtc gcgccagtga gttctgcaag gaacgcacaa
      841 cgctgatatt ggctggaacg ccgttagcgg tataacccgc ccgatgcgtt ttatattcgg
      901 tcagctggcg ttgttggttt tgtttcagat tcggtaaggc caaatcttca aagagcagat
      961 caagatactc atctttcgaa atctgaaaga caaattcatc ctgaccttca ccatcctggc
     1021 tggcctggcc ctgaccactg ccggaacctc cgccgccacc ctggggacgt tcaattcggt
     1081 cgttctggac gaaatggtca ttgcccggat gtacgcggtg gcgcaggccg ccacgcccct
     1141 gatgaaacat cggttcgcta atatcttccg tgggaatgga tacggattcg ccgctgtcga
     1201 cgtcagtcac cgaacgctta ttaatggcct cggagatcga ctgtttaatt tgcgctttat
     1261 aacggcgtaa aaaacgctgg cgattcacca tgcttttgtt tttgccgttc agacgccggt
     1321 caataaaccc ggtcatatgc cccccgtact acatttgcca actgtatgct acgcctgtgt
     1381 tgccatttct ctggcccacg gttcgctgtc gttataggcc cggtagcatg tgtaccgggc
     1441 cagggttgtt aagacgattt acgtacgcgc aaataccatt cgcacagtaa acgcacctgt
     1501 ttacgggtgt agcctttttc catcatacgg tcgacaaagt cgtcgtgttt cttctgctcg
     1561 tcggttgacg ttttggcgtt aaacgagata accggcaaca gctcctcggt attggagaac
     1621 attttcttct cgataaccgt gcgcagtttt tcatagctgg tccagttcgg attgcgtccg
     1681 ctgttattcg ctctggcgcg cagtacgaag ttaacaatct cgttgcggaa atcttttgga
     1741 ttactgatcc ccgccggttt ctcgattttc tccagctcgg cgttaagaga ctcgcggtca
     1801 aacagctgcc cggtatccgg atcgcgatac tcctgatcct gaatccagaa atccgcgtag
     1861 gtaacataac ggtcgaaaat gttttgccca tattcggaat aggattcaag gtaggccgtc
     1921 tggatctctt tgccgataaa ctcggcatat ttcgggatca gataaccttt caggaactcc
     1981 agatagcgtt ctgcctgctc ttgcgggaac tgctcgcgct cgatctgctg ttctaggacg
     2041 tagaacagat ggaccgggtt tgccgccact tctacatgat cgaagttgaa cacgcgggag
     2101 aggatcttaa acgcaaaacg cgtcgacaga cctttcatcc cttcatcgac accggcgtag
     2161 tcacgatatt cctgatacga cttggctttg ggatcggtgt ctttcagact ttcgccatca
     2221 taaacccgca tctttgaata aatgctggag ttttctggct ctttcaggcg cgaaagaatg
     2281 gaaaaacgtg acagtgtttc cagcgtgcca ggggcgcatg gggcgtgagt caattcactg
     2341 tgattaagca atttctcgta gattttgatc tcttcggaaa tgcgtaagca atacggcacc
     2401 ttcacgatgt aaacacggtc gaggaaggct tcgttgtttt tgttattacg gaaagtgacc
     2461 cattcggatt cgttcgagtg ggcgagaata atcccgttga acggcagggc ggagatacct
     2521 tccgtcccgt tgtagttacc ttcctgagta gcggttaaca gcggatgcag cactttaatc
     2581 ggtgctttaa acatctcgac aaattccatg atcccctgat tggcgcggca cagcgcaccg
     2641 gaatagccgt aggcgtccgg gtcattctgc gcgtagtgtt cgagtttacg aatatcgact
     2701 ttccctacca gcgcggagat gtcctggttg ttctcatcac cgggttctgt tttggcgata
     2761 gcaatttgtt gcagaattga cggccagacc ttcactaccc ggaacttagt gatatcgcca
     2821 ccaaattcat gcaggcgttt tgccgcccac ggcgacatga tggtgccgag atagcggcga
     2881 ggaatgccat actctttttc cagaatctgc gcatcttcct gcggattgaa aagacagaac
     2941 ggatgatcgt tgaccgggct acgctcaccg ttggcgctca atacataaat cggtacgagc
     3001 tgcattaatg atttcagtcg ctcagcaagc gatgatttac ccccacccac aggccccagc
     3061 agatacagga tttgtttctt ctcttccagc ccctgagccg cgtgtttcag ataagagaca
     3121 atctgttcaa tcgcgtcttc catgccgtaa aactcttcaa atgccggata acgtgcaatg
     3181 acccggttag aaaagagtcg agaaagtctg ggttcctggg ctgtatcgac catgacaggc
     3241 tcaccgatag ccatcaatag ccgctcagcc gcgttggcat aagcactgcg atcttgccga
     3301 caagtggtaa gaaactcctg cagtgtgaac tcttcgtcct tggcagcttc atatcgctgg
     3361 cgatagtgat cgaatatatt catggtatgc cgtccttttg tatactctgg gaacatttgt
     3421 gaatcagcac ttttagcgta attcttcaac gggataaaaa atatgctccg tcctatttgt
     3481 tatgagcgag tatcaagcat tcagcaagtt gatatgggta aaggtttgga cgaccaacga
     3541 tcatcattga ctgcatactt agataaaaat gctgataagt tcactgacga aaggattttc
     3601 attactgatg cggggctttc cgcattcaaa aatgccaata tctctccaga gtctcagctt
     3661 gggatatttt tgcaagatgt gggcaaccgc aagtacggcg taggtgacgc acttgtggtt
     3721 ttatcgctgg atcgtctatc gcgtcgatca agctgggccg aaagtactat ccagtacatc
     3781 gtgaatagtg ggattgagat tcacgatatc tctacaaacc ttgtgttacg ggttgatgat
     3841 cctatgtcaa aaattcacat ggaactgata cagcaccgtt cacacaatga gtcattgatg
     3901 aaatcggtac gtgcaaaatt ggcatgggat aacaaaatca cacgtgcagt acagaacggg
     3961 gaagtaatct ccaaccgtat gcctaattgg ttgagaaatg tagataacaa gtaccaagtt
     4021 ataccggaac aggcagaact gattgttaaa tgtttcatgt ggtataggga agggttgagt
     4081 actggcgaga ttgttaaacg tatcggtaga aagtggcaga tggtcactgt gtccaggctg
     4141 atacgtgatc gccgtttgtt gggcgagcat gaacgttaca acggtgaggt agtaccaaac
     4201 gtttacccga aaatcatcga cgaagagttg ttcctgacag cgaatagaat gatggatcgc
     4261 gttatgttgg agaagaagaa gccagcagag gatctattac ttgaaccaga agttgtccga
     4321 gagattttta aactttatga gacaggcctt ggttcagggg cgattgtaaa acgcttgccg
     4381 aaagggtgga gtaccgtcaa tgtactgagg gtactacgtg ataagaaagt agttaccatg
     4441 aagattatcg ataacctcac ctttgaacgg gctaatgaga agttatcgcg gaatggtata
     4501 gctaatcgta tccgtaagga cattaccatt gctcaggatg attacattac taacctcttc
     4561 ccgaagatgc tgaaatgcgg tcactgtggg ggcaatattg caattcacta taatcatgta
     4621 cgaactaagt atgttatttg tcgtaacagg gaagaaaaga agatctgcga tgctaaatct
     4681 atccagtaca tgagaattga aaagaacatt ctacagcttg tcaaaaatgt agacttcaat
     4741 aaattgatga tggaaggtaa gtcggattca gattcattgc ttgcatcatt gcgagaagag
     4801 ttaagtactt tacagaaaga ggcagaaacc tatcaggtta aaattaatga acgtaaggca
     4861 gcaggtaaga aagcctcgtt accattaatg acgggttt
//
LOCUS       DAZOZY010000253         4870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZOZY010000253 DAZOZY010000000
VERSION     DAZOZY010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.256"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..677)
                     /locus_tag="ACHURQ_003914"
     CDS             complement(<1..677)
                     /locus_tag="ACHURQ_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0825570.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPMGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREA"
     gene            906..1514
                     /locus_tag="ACHURQ_003915"
     CDS             906..1514
                     /locus_tag="ACHURQ_003915"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR0825571.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1612..3003
                     /gene="selA"
                     /locus_tag="ACHURQ_003916"
     CDS             1612..3003
                     /gene="selA"
                     /locus_tag="ACHURQ_003916"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR0825572.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3000..4844
                     /gene="selB"
                     /locus_tag="ACHURQ_003917"
     CDS             3000..4844
                     /gene="selB"
                     /locus_tag="ACHURQ_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR0825573.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1069 a         1280 c         1443 g         1078 t
ORIGIN      
        1 gcttcgcggt ggaacgtctg tgtgcgcccg aagcggtcca ccagcccggt cagtacccgg
       61 tacggcggca gcggcgcagg cagcacctca tccgcttccg gcacccgctc acaccagccg
      121 agcagccacc acggcccctg cggactgttt gtcgccagat aacgatgcgg acttaagcgg
      181 agttcttccg gcagcgcctg ccagagtgcg gccagccggt gaccttcatc cagtttcgcc
      241 acgccgccgc gcaccagcca gagtgattcg ctgcggctgt aaccgtcctc accgggaaac
      301 aggtgctcaa aatacaggct tctgccgccg ttatcactga gtatcagtgt gttatcgcgc
      361 agctgtaagc ggatatccgc aggcattttc cagccggggc cgaggctccc catcggcgcg
      421 ggcgtttttg tccggtaact gctgtaggtg cgggagagga tgaacggcag cggggcgggc
      481 agggcgatgt cggtttcacc gggaaggacc tttgcaccga gcaggggatt gaccggatgg
      541 ccggacgtca ctccgccggg gcacaccgaa caggccacac cggtgggggc accgatgcgc
      601 accccggctg aaccctgaac aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgct
      661 gccggttttc cgctcatctg ctcatccttg ttttatcggg ttatttgtct gcttccaggc
      721 aaaaccctac agatttataa gaaagcctta agtgagatat ccatcagata gaaacaatta
      781 ggaaaatatt tatatcacca tgataaatat gtgatttatt tggaagtaac aaaccatcaa
      841 cattttctgc cctttttccc atcaccaggc catactgata tttttcctcg cacaaaggac
      901 tcggcatgaa actcgtcggt agctacacca gcccgtttgt acgcaaactt tctattctgt
      961 tgttagaaaa gggcataact ttcgaattta ttaatgaact gccctataac gcggacaacg
     1021 gcgtggcgca atttaacccg ttaggaaaag tgccggtgct ggtgaccgaa gagggcgaat
     1081 gctggtttga ttcgccgatc atcgctgaat atattgagtt aatgaatgtc gctccggcga
     1141 tgttgccgcg cgatccgctg gagtcgttgc gggtgcgcaa aattgaggcg ctggcggatg
     1201 gcattatgga tgccgggctg gtatcggtgc gtgaacaggc gcgtccggcg gcgcagcagt
     1261 ctgaagatga attgttacgc cagcgggaga aaatcaaccg cagtctggat gtgctggaag
     1321 gatatctggt cgatggcaca ctcaaaaccg atacggtcaa tctggcgact atcgccattg
     1381 cctgtgctgt cggatatctc aatttccgcc gcgttgcgcc aggttggtgt gtcgaccgcc
     1441 cacacttagt caaactggtc gaaaacctgt ttagccgcga aagttttgct cgcaccgaac
     1501 cgccaaaggc ttgatgcgcg atatgtcctc ctgacccatc tcacgttaca atccgtggtt
     1561 atgttaaacg cccttctccg tgtgagaggg ccttgatcag ccaggtttcc tatgacaacc
     1621 gaaacgcgtt ccctctatag tcaacttccg gctattgatc gcttattgcg cgatagctcc
     1681 ttcctttctt tgcgtgatac ttatggtcac acccgcgtgg tggaattgtt gcgtcagatg
     1741 ctcgacgaag cgcgagaagt gattcgtgac agccagacgc ttcctgcgtg gtgtgaaaac
     1801 tgggcgcaag aagtcgatgc ccggttgacg aaagaggcgc agagcgcgct gcgtccggtg
     1861 atcaacctga cgggaaccgt gctgcatacc aaccttggac gagctttaca ggcggaagcc
     1921 gcggtggaag ccgttgcgca ggctatgcgt tcgccagtga ccctcgagta cgatctggac
     1981 gacgccggac gcggacatcg cgatcgggcg ctggcgcaac tgctgtgccg tattacgggg
     2041 gcggaagatg cctgtatcgt caataacaat gcggcggcgg tgttattgat gttggcggcc
     2101 actgccagcg ggaaagaggt ggtagtatct cgcggcgaat tggtggagat tggcggcgcg
     2161 tttcgtattc ccgatgttat gcgtcaggca ggctgcaccc tacacgaagt agggaccacc
     2221 aaccgcacgc acgcgaatga ttatcgtcag gcggtgaatg aaaataccgc actgttgatg
     2281 aaagtacata ccagtaacta cagcattcag gggttcacca aagcgataga tgaagcggaa
     2341 ctggtggcgc tcggcaaaga gctggatgtt cccgtagtga ctgatttagg cagtggctcg
     2401 ctggtcgatc ttagccagta cggtttgccg aaagagccaa tgccgcagga gttgattgcg
     2461 gcgggcgtca gtctggtgag cttctccggc gacaagttgt taggcgggcc gcaggcaggg
     2521 attatcgttg gtaaaaaaga gataatcgcc cgcctgcaaa gccacccgct gaagcgtgca
     2581 ttacgcgcgg ataaaatgac cctcgcggcg ctggaagcca cgttgcgtct ttatttacac
     2641 cctgaagctc tgagtgaaaa attaccgacc ctgcgcctgc ttacccgcag cgcagaggtc
     2701 attcaaatcc aggcacaacg tttacaggct ccccttgccg cacattacgg cgcggagttt
     2761 gcggtacagg ttatgccatg tctttcgcag attggcagtg gttcgctgcc ggttgatcgc
     2821 ctgccgagcg cggcattaac gtttacaccc catgatggac gcggtagcca ccttgagtca
     2881 ttagccgccc gctggcgtga attgccagtg ccgatgattg gtcgtattta tgacggacga
     2941 ttgtggctgg atttacgctg ccttgaagat gagcaacggt ttttggagat gttgttgaaa
     3001 tgattattgc gactgccgga cacgttgacc acggcaaaac aaccttattg caggtgatta
     3061 ctggcgtaaa tgctgaccgt ctgccggaag aaaaaaagcg cggcatgacc atcgatctcg
     3121 gctatgccta ctggccgcag ccggatggtc gcgtgcctag ttttatcgac gtacccggtc
     3181 atgaaaagtt tctttccaac atgctggcgg gcgttggtgg tatcgatcac gcgctgttgg
     3241 tggtggcgtg cgatgacggc gtgatggcgc agacccgtga gcatctggcg attttgcagc
     3301 tgaccggtaa cccgatgctg acagtggcgc tgaccaaagc cgatcgcgtg gacgaagcgc
     3361 gtgttgatga ggttgaacgc caggtaaagg aggtgctgcg ggaatacggt tttgctgagg
     3421 caaaactgtt tatcaccgca gcaaccgaag gtcggggaat tgatgccctg cgcgagcatc
     3481 tgcttcagtt accaggacgt gaacacgcca gccaacatag tgtccgcctc gcgatcgacc
     3541 gcgcatttac cgtaaaaggt gccgggctgg tcgtcaccgg tacggcgtta agcggggaag
     3601 tgaaggtagg cgattcactc tggctgactg gtgtaaataa accgatgcgt gtacgtgcgc
     3661 tgcatgcaca aaaccagcca acagaaaccg cccatgccgg gcagcgtatc gcgcttaaca
     3721 tcgcgggtga tgcggaaaaa gagcagatta accgtggcga ctggctgctt gccgatgcgc
     3781 cgccagagcc gttcacacgg gtgattgtcg agcttcaaac ccatacgccg ctgacccagt
     3841 ggcagccgct gcatattcac cacgccgcca gccacgttac gggacgcgtt tcgctgctgg
     3901 aagataacct tgccgagctg gtgttcgaca cgccgctgtg gctggcggat aacgatcgct
     3961 tagtgctgcg cgatatctcg gcccgcaaca cgctggcggg ggcgcgtgtg gtgatgctta
     4021 acccgccgcg tcgcggtaaa cgtaagccgg aatatctgca atggctggcg tctcttgcac
     4081 gggcgcagag cgatgccgat gcgttatctg ttcatctgga acgcggcgcg gttaaccttg
     4141 cggatttcgc ctgggcgcgc cagctcaacg gcgaagggat gcgcgaattg ctgcaacagc
     4201 ctggctatat tcaggctggt tatagcttgt tgaatgcgcc ggttgccgcc cgctggcagc
     4261 ggaaaattct cgacacatta gcgacttatc atgagcaaca tcgcgatgaa cctggtcctg
     4321 ggcgcgaacg tctgcgacgt atggcgttgc caatggaaga tgaagcgctg gtactgttgc
     4381 tgattgaaaa gatgcgcgaa agcggcgaca tcctcagtca tcacggttgg ctgcatctgc
     4441 cggatcacaa agcgggcttc agcgaagagc agcaggccat ctggcaaaaa gcagagccgc
     4501 tgtttggtga cgagccgtgg tgggtgcgtg atctggcaaa agagacgggt accgacgagc
     4561 aggcaatgcg cctgacgcta cgccaggcgg cgcagcaagg aataattacc gcgatcgtca
     4621 aagatcgtta ttaccgtaac gatcggattg tcgaatttgc caatatgatc cgcgatctcg
     4681 atcaggagtg tggttcaacc tgcgcggcgg atttccgcga tcgcttaggc gtaggccgaa
     4741 agctggcaat tcagattctg gaatattttg accgcattgg ctttacgcgt cgtcgtggaa
     4801 atgatcattt attgcgcgac gcattattat ttccggaaaa ataaggaaat gattaattaa
     4861 tttttaaaat
//
LOCUS       DAZOZY010000254         4848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZOZY010000254 DAZOZY010000000
VERSION     DAZOZY010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.257"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHURQ_003918"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHURQ_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR0825574.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHURQ_003919"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHURQ_003919"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR0825575.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHURQ_003920"
     CDS             2475..4124
                     /locus_tag="ACHURQ_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR0825576.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..>4848)
                     /locus_tag="ACHURQ_003921"
     CDS             complement(4278..>4848)
                     /locus_tag="ACHURQ_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0825577.1"
                     /translation="TFLDYSNFYMAYMAEANLYKVIAPYVNLFKADLSFSKLDLINFE
                     HADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTDSNMANVNFNNANLSNCH
                     FNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDC
                     TSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1020 a         1150 c         1338 g         1340 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac caaggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggta
//
LOCUS       DAZOZY010000255         4844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZOZY010000255 DAZOZY010000000
VERSION     DAZOZY010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.258"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(34..954)
                     /locus_tag="ACHURQ_003922"
     CDS             complement(34..954)
                     /locus_tag="ACHURQ_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0825578.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHURQ_003923"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHURQ_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR0825579.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="ACHURQ_003924"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="ACHURQ_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR0825580.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHURQ_003925"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHURQ_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR0825581.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1307 a         1081 c         1140 g         1316 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAZOZY010000256         4843 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZOZY010000256 DAZOZY010000000
VERSION     DAZOZY010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.259"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(88..2961)
                     /gene="gcvP"
                     /locus_tag="ACHURQ_003926"
     CDS             complement(88..2961)
                     /gene="gcvP"
                     /locus_tag="ACHURQ_003926"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR0825582.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(3080..3469)
                     /gene="gcvH"
                     /locus_tag="ACHURQ_003927"
     CDS             complement(3080..3469)
                     /gene="gcvH"
                     /locus_tag="ACHURQ_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR0825583.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(3493..4587)
                     /gene="gcvT"
                     /locus_tag="ACHURQ_003928"
     CDS             complement(3493..4587)
                     /gene="gcvT"
                     /locus_tag="ACHURQ_003928"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR0825584.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
BASE COUNT         1092 a         1365 c         1257 g         1129 t
ORIGIN      
        1 gcgaggggac tgtctgagca cttttgtact ttgtcatctg actaaaaagg cgccgaagcg
       61 cctttagaaa atagtcgaat cagtgaatta ctggtattcg ctaatcggta cgcaggagca
      121 gaacaggtta cggtcgccgt aaacatcatc cagacgtttc accgtcggcc agtatttgtc
      181 tgccacgcct gccgggaata ccgcaacttc acggctgtac ggatgcgccc actcggcgac
      241 cagttcgctc tgaatgtgcg gcgcgttcac cagcgggtta tcttccagcg gccagacagc
      301 ggctttcacc tggtcaattt ctgcgcggat agccagcatc gcgtcgataa agcgatccag
      361 ttccactttg ctttcagatt cagtcggttc aaccatcagc gtacccgcca ccgggaacga
      421 catcgtcggc gcgtggaaac cgtagtcgat caggcgcttg gcaatatcca gctcgctgat
      481 gccggtttct tctttcagcg ggcgaatatc gagaatacat tcgtgcgcca cgcgaccgtc
      541 gcgaccggta tacagcaccg ggaaggcatc ctgcaggcgg ctggcaatat agttggcgtt
      601 gaggattgcc acctggcttg cttttttcag cccttctgcg cccatcatgc ggatgtacat
      661 ccagctgatt ggcaggatag aggcgctacc gaacggtgcc gcagaaactg cgccctgacg
      721 ggttaacatg ccttcgattt gcaccacgct atgacccggt acaaacggtg ccagatgcgc
      781 tttcacaccg atcggtccca tacccggacc accaccgccg tgcggaatgc agaaagtttt
      841 atgcaggttg aggtgcgaaa cgtccgcgcc aataaagccc ggcgaggtga tgccaacctg
      901 ggcgttcatg ttcgcgccat caaggtaaac ctgaccgccg aactgatgca cgacttcaca
      961 cacttcacgg atcgtttctt catacacgcc gtgggtagaa ggataagtca ccatgataca
     1021 ggagaggtta tcgcccgcct gttccgcttt cgcgcgcaga tcagtcagat cgatgttgcc
     1081 gtttttatca cacgccacaa ccaccacctg cattcctgcc atatgtgcag aagcggggtt
     1141 agttccgtgc gcagaagccg ggatcaggca gatatcgcga tgcccttcgt tgcggctttc
     1201 atgataatga cgaatcgcca gcaggcccgc gtattcgccc tgtgcgccgg agttcggctg
     1261 catacaaacg gcgtcgtaac cggtcagttt caccagccag tcagccagct gcgcaatcat
     1321 ctgctgataa ccttcagcct gttccggcgg gcaaaacggg tgcagttcgg caaattccgg
     1381 ccaggtgatt gggatcatct cggcggcggc gttcagtttc atggtgcagg aacccagcgg
     1441 gatcatcgcc tgattcagcg ccagatcttt acgctccagc gagtgcatat agcgcatcat
     1501 ttcggtttcg ctgtggtagc gattaaacac cggatgggtg aggatttcgt cgtcgcgcag
     1561 catcgcaggc tggatagagc ggctgtcgtg agccacgtct ttgtccagcg tgtcgatctc
     1621 cagcccgtgg ttatcgccca gcagcacgct gaaaagctgc attacgtttt cacgcgtggt
     1681 ggtttcatca agggtgatcc ccacggcgtt caggatatcg ctacgcaggt tgatttcagc
     1741 cgcttcggca cgcgtcagta cgcccgcttt gtcggccact tccacacaca aggtgtcgaa
     1801 atagtgcgca tggcgcagtt tcagaccttt ttgttgcagg cccgccgcca gaatatcggt
     1861 cagacggtga atgcggttag cgatacgttt caggccaacc gggccgtgat aaacggcata
     1921 caggctggcg atgtttgcca gcagtacctg ggaagtacaa atgttggagt tcgctttctc
     1981 acgacgaata tgttgctcgc gagtctgcat cgccatgcgc agcgcggtat tgccagctgc
     2041 atctttcgat acaccgataa tacggcccgg cattgagcgt ttgtattcat ctttcgccgc
     2101 aaagaatgcc gcgtgtgggc caccgtagcc catcggcacg ccgaagcgtt gcgccgaacc
     2161 aaaaacaata tccgcgccct gtttacccgg cgcagttaac agcaccagcg ccataatatc
     2221 ggcggcaacg ctgaccacaa ttttgcgtga tttcagttcg ctaataagcg cagtgtagtc
     2281 gtgaatttca ccggtagtgc ctacctgctg taacagcacg ccgaagacgt cctgatggtc
     2341 gagcactttt tgcgcgtcat cgacaatcac ttcaaaacca aaggtttcgg cacgagtacg
     2401 gaccacatcc agcgtttgcg gatgcacatc ggaagccacg aagaagcggt tggcattttt
     2461 cagtttgctg acgcgtttcg ccatcgccat tgcttcggcg gcagcggtgg cctcgtccag
     2521 aagagaagca gaggccatat ccagtccagt caaatccagc gttacctgct ggaagttgag
     2581 cagtgcttca aggcggccct gggagacttc aggttgatac ggagtgtacg cggtatacca
     2641 gcccggattt tccagcatgt tacgcaggat aaccggcggt agctgcacgg cggtgtaacc
     2701 catgccgatg taagacgtga agcgtttatt gcgactggca atagccttga gttctgccag
     2761 tgcggcgtat tcggtcgccg gtgcgccaac ctgcggcggg gtcgcaagct gaatatcttt
     2821 cggtacaatc tggccggtca gcgcgtttaa cgattgtgcg ccaacggcat tcagcatttc
     2881 ttgctgttgc gcggcgtccg gtccgatatg gcgttcaata aaagcgccgc tgttttcaag
     2941 ctggcttaac gtctgtgtca tgagcgatgg ttcctgaaac gtgcagtgaa ttgtgaacct
     3001 ctctccttac gaagagagtg agggtgaggc ataaattttc ctcaccctga tcctctcccg
     3061 cagaagagga ataaagccgt tactcgtctt ctaacaatgc ttcgtatgcg gtcgcatcca
     3121 gcagtgattc cagttcgctt tcatcgctgg ctttgatttt aaagatccag ccacctgcat
     3181 acggttcgct gttcaccagt tccggggaat cgctcagtgc gtcgtttacc gccacgattt
     3241 caccgcttac tggcgcataa atgtctgacg ccgcttttac tgattcggca accgcgcagt
     3301 catcgcccgc gctaaccgtt gcgcccactt ccggcaggtc aacaaacacc atatcgccta
     3361 acagctcctg agcatgttcg gtaataccaa cggtgtaagt gccgtcggct tctttacgca
     3421 gccattcgtg ttctttgctg tatttcagtt ctgctggtac gttgctcatc aatcaatctc
     3481 caaaaaagta aatcacgcga cggctttgcc gttacgcaca aaaacaggtt ttgtcacttt
     3541 aaccgacatt tcacggttgc gaatttgcac aatcgccgtt tcgccaatac cttccagcac
     3601 gcgcgccagc gcaatgctgt aacccagcgt cggggagaaa gtaccgctgg tgataatgcc
     3661 ttcatgctgg ttgccctgcg catcggtaaa gcgtaccggc agttcattac gcagcacgcc
     3721 tttttcggtc atcaccagac caaccagttt ttctgtacca tgctcacgct gcacttccag
     3781 ggcttcacga ccgataaagt cacgatctgc cggttcccag gcgatagtcc agcccatgtt
     3841 ggcggctaaa ggagaaatag tttcgtccat ctcctgacca taaagattca tgcccgcttc
     3901 cagacgcagc gtgtcacgcg cgcccaagcc acatggctta acacccgctt ccaccagcgc
     3961 acgccagaaa tcggccgctt tttcattggg cagcgcaatt tcatagcccg cttcaccggt
     4021 ataaccagtg gtggcaataa acagatcgcc cgcctgcacg ccaaagaacg gtttcatccc
     4081 ttccaccgcc tgacgctggg cgtcattaaa cagtgtggca gcttttgcct gcgcattcgg
     4141 cccttgcacg gcaatcatgg aaaggtcatc acgaacggta atttcgatgc cgaaaggttc
     4201 agcgtgttgg gtaatccagg agaggtcttt ttcgcgggtg gcggagttaa caacgaggcg
     4261 gaagaaatct tcagtaaagt agtagacgat gaggtcatct atcacaccgc cagaggcatt
     4321 caacatcccc gagtaaaggg ctttgccgct tttggtgagc ttcgccacat cgttcgccag
     4381 cagataacgc agaaactccc gggtgcggct gccgcggaga tcgacgatgg tcatatgtga
     4441 cacatcaaac attccggcat cggtacgtac cgcatgatgt tcgtcgattt gcgaaccgta
     4501 atgcagcggc atcatccagc cgtggaaatc caccatgcga gcgccgcaaa gcgtgtgttg
     4561 ttcgtacaaa ggagtctgtt gtgccatctt gtcctcattg aataagcggg gctgacaact
     4621 tttcatggtg aaattatcac cacgaaaccc agcatcggag ccactcccgg tccccaacgc
     4681 aatcgttctc ttttgcctga acttaccacc gaaacagact gttaaccata aggtaaaatt
     4741 gatcatcaca ttagcttatg gttaaaaaac gccaaaatcg cgacagaata aaaaaccaaa
     4801 aaacacacca gtttctatat aaagatgatg ccattagaaa gct
//
LOCUS       DAZOZY010000257         4821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZOZY010000257 DAZOZY010000000
VERSION     DAZOZY010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.260"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(56..2125)
                     /gene="glyS"
                     /locus_tag="ACHURQ_003929"
     CDS             complement(56..2125)
                     /gene="glyS"
                     /locus_tag="ACHURQ_003929"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR0825585.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2135..3046)
                     /gene="glyQ"
                     /locus_tag="ACHURQ_003930"
     CDS             complement(2135..3046)
                     /gene="glyQ"
                     /locus_tag="ACHURQ_003930"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR0825586.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3141..3440)
                     /locus_tag="ACHURQ_003931"
     CDS             complement(3141..3440)
                     /locus_tag="ACHURQ_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR0825587.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3615..4610
                     /gene="wecH"
                     /locus_tag="ACHURQ_003932"
     CDS             3615..4610
                     /gene="wecH"
                     /locus_tag="ACHURQ_003932"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR0825588.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4652..>4821)
                     /locus_tag="ACHURQ_003933"
     CDS             complement(4652..>4821)
                     /locus_tag="ACHURQ_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0825589.1"
                     /translation="MVGLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPK
                     ETQVTQEEYSE"
BASE COUNT         1101 a         1269 c         1223 g         1228 t
ORIGIN      
        1 tttagcgata ataaaaaaag cctgccagat ggcaggcttt ttaataacgg cgttattact
       61 gcaacagcga aatatccgca acgcgcagga acagttcgcg cagtttctcc agcatggtca
      121 gacggttgag acgcaattct ttgtcatcaa ccatgaccat cactttatcg aagaaagcat
      181 caaccggctc acgcagctct gccagttcga ccagcgcatc ctggtaacga ccttccgcaa
      241 agtacggctc cagcttgtca cgtagcacca caacctgcat cgccagttta atttcttccg
      301 gctctttcag ggtagaggca ttcacgcggt cgctcagcac ttcgtcagat ttcgccagaa
      361 tgttagatac acgtttgttc gccgccgcca gtgcagcagc tgcatccagg gtacggaaat
      421 gcgataccgc tttcatacgg gcatcgaaat cagccggacg agtcggacga cgcgccagta
      481 ccgcctggat ggtgtcaacg gtgtaacctt cgtcctgata ccaggcgcgg aagcgaccga
      541 gcataaagtc gataacatca tcaactacgt tggcgttagt cagcttatcg ccatacagac
      601 gcactgcttc ttcggtcagc gtttgcagat caaggttgag gttcttctca acgataattc
      661 gcagcacgcc aagtgcggca cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct
      721 gaccgatacc gaagataccc gccagagtat ccatcttatc agcaatcgcc agcgcacaag
      781 ctaccgggtt ggacggcagg tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg
      841 cgacggctac gtcttccgct tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt
      901 gggtgtcggt gaactcgaag accatgttgg tcatcaggtc acacttagac agcaggcccg
      961 cacgggttgc gtggttaacg tcagcgccaa tctgttcagc aatccagcca gccagcgcct
     1021 ggatgcggtc agttttgtca cgcagcgtac ccagctgttg ctggaacagc acggtttgca
     1081 ggcgcggcag gttatcttcc agacgttttt tacggtcggt attgaagaag aactcggcat
     1141 ccgccagacg tggacgaacg actttctcgt taccagagat aatttgctgc ggatctttcg
     1201 attcgatgtt ggcaacgaag atgaagttcg gcagcagttt gccatcgttc gcatacaccg
     1261 ggaagtattt ctggtcacct ttcatggtgt aaaccagggc ttcagccggc accgcgagga
     1321 atttctcttc gaattttgcg gtcagaacga ccggccactc caccagcgaa gccacttctt
     1381 ccagcaggct ttcgcttaag tcagcgttac cgccaatctt acgcgctgct tcttcggcat
     1441 cggctttaat cttcgcttta cgttcttcgt aatcggcgat gactttcccg cgctcacgca
     1501 gaatttccgg atactgatcg gcgttgtcga tggtgaattc cggttcgccc ataaagcggt
     1561 ggccgcgaat cacgcgatcg gactgaatgc ccagaatggt tgccggaatg actttgtcgc
     1621 ccagcagcag ggtcacggtg tgcaccggac gcacgaagtg cacgtcgctt gcgccccaac
     1681 gcatcagttt cgggatcggc agtttcgcca gcgaagtcgc aaccatattc ggcagtagtg
     1741 cttcagtgct ttcgcccttc acatgggcgc gatacagcag ccattcgcct ttatcggtag
     1801 tcagacgctc agcctggtca acggtaatac cgcaaccacg cgcccaacct tctgccgctt
     1861 tgctcggttt gccttcagcg tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt
     1921 cacgatctgg ttgcgcttcc gccaggttag ctactttcaa cgccagacga cgcggagcag
     1981 caaaccattg aacggtgccg tgtgcgaggc cagcgttatc cagctccgca gtaaagttcg
     2041 cagcaaagga ctcagccagg ctgcgcagtg cttttggtgg cagctcttca gtgccgattt
     2101 ccaccagaaa agttttctca gacatagccg cctcttactt atctttgttg cacatcggga
     2161 agccgagggc ttcacgggaa gcgtagtatg cttctgccac tgctttggtc agggtgcgaa
     2221 tgcgcagaat gtagcgctga cgctcagtga cggagatggc tttacgcgca tccagcaggt
     2281 tgaagctgtg ggcggctttc agaatacgct cgtaggctgg cagcggcagc ggattttcca
     2341 gcgccagcag ctgctgcgct tctttctcgt actgctcgaa gcaggtgaac aggaaatcca
     2401 catccgcgta ttcgaagttg taagtggact gctccacttc gttctgatgg aacacgtcgc
     2461 cgtaggtggt tttacccagc gggccgtcgc tccagaccag gtcgtaaacg ctgtctacgc
     2521 cctgaatgta catggccaga cgttccagac cgtaggtgat ctcgccggta accggtttac
     2581 actccagacc accaacctgc tggaagtaag tgaactgcgt cacttccatg ccgtttagcc
     2641 acacttccca gcccagtccc caggcaccca gagtcgggtt ttcccagtta tcttccacga
     2701 aacggatgtc gtgaatagtc gggtccatgc ccagctcttt cagagaaccg aggtacagct
     2761 cctgaatatt gtccggcgat ggcttaatga ccacctggaa ctgatagtag tgctgtaaac
     2821 ggttggggtt ttcgccgtag cgaccatcgg tcgggcgacg agaaggctga acataagcag
     2881 ccgccatcgg ttctggcccc agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc
     2941 cgacttccat gtccaatggt tgaacaatgg tgcagccctg gcgagcccag taatcctgta
     3001 aggtcaggat caagccctgg aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg
     3061 gatacgtgtg gatttaaagg aagggatcag tatacccgct ggatggaaga tatacagtac
     3121 gaaacgggaa aaagcagggc ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca
     3181 aaagaacaga caaactgctc tttacggaag gtataaccgc gcatttcata gcttccctgg
     3241 aactgttcga aggcggtgac gtcgattttt tgctcgccgg tgttataacg ttttgccgcc
     3301 tgatccttgc ataacgcttc catattcaac gaacgcagag gatcaacctt tacccgttgt
     3361 gctttctgca cgggtgacgg tgtagaccaa cccactagca ccataagcgc cattgccgga
     3421 aagaaagcgt tcatcatcat taaattaccg tctggtttgc aggtgagtct tattatttat
     3481 atcggtatag tggctgatat cttacgaatt tccttaagcc tctataagct gtagtgataa
     3541 aactcagatt attccagtgc gccaaccaaa tgatttatcg gtcagacttt ttttaaccag
     3601 atacagagac acagatgcag cccaaaattt actggattga taacctgcga gggatagcgt
     3661 gtttaatggt ggtgatgatt cacaccacta cctggtatgt gaccaatgct catagtgtta
     3721 gccccgtcac atgggatatc gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc
     3781 tatttttcat gatttccggc tatctctttt ttggcgaacg cagcgcccag ccgcgccatt
     3841 tcttgcgtat cggcttatgt ctgatttttt atagcgcaat cgcactgctc tacattgcgc
     3901 tgtttacctc catcaatatg gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt
     3961 accacttgtg gtttttcttc gcgattgcgg tgatttatct ggtttcaccg ctgattcagg
     4021 tgaagaacgt cggcggaaaa atgttgctgg tgctaatggc ggtgattggc attatcgcta
     4081 acccaaacac agtgccgcag aaaattgacg gttttgaatg gctgccaatt aacttatata
     4141 tcaatggcga tactttttac tacattctgt atggcatgtt gggccgcgct atagggatga
     4201 tggacacaca gcataaagca ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt
     4261 ttattatctc tcgcgggaca ttatatgaat tgcagtggcg cggaaatttt gccgatacct
     4321 ggtatcttta ctgtgggccg atggttttta tctgcgcaat cgcgctattg actctggtta
     4381 aaaacacgct ggatacgcgt accattcgcg gacttggctt aatctcccgc cattcattgg
     4441 gtatatacgg attccacgcc ttgattatcc atgcgctgcg cacccgggga attgagctta
     4501 aaaattggcc aatactggat attatttgga tcttttgcgc gacgttggca gcgagtttgt
     4561 tactttctat gctggtacaa cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc
     4621 gttcacattg gactgaccgg gcttacgtga attattcgct gtattcttcc tgggtaacct
     4681 gtgtttcttt tggcgggttt attccggcat cacgaatatt cttctgcacc gctactgcac
     4741 caaacaagct taagaagaaa gccagtccat aaaaaccttt ttcactgagt aataatgtcg
     4801 cattccacag acctaccatc a
//
LOCUS       DAZOZY010000258         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZOZY010000258 DAZOZY010000000
VERSION     DAZOZY010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.261"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHURQ_003934"
     tRNA            6..81
                     /locus_tag="ACHURQ_003934"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHURQ_003935"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHURQ_003935"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHURQ_003936"
     CDS             complement(299..1057)
                     /locus_tag="ACHURQ_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR0825590.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHURQ_003937"
     CDS             complement(1065..2168)
                     /locus_tag="ACHURQ_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR0825591.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHURQ_003938"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHURQ_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4459)
                     /locus_tag="ACHURQ_003939"
                     /pseudo
     CDS             complement(3427..4459)
                     /locus_tag="ACHURQ_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1317 a         1241 c         1147 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcatggcga
     3721 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3781 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3841 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3901 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3961 ccttgaaata gtcggcgacg ttgagttcgg tatctgtacc cgcctgaata cagacggtag
     4021 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4081 cgtcgtaatc agtgacgcca gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc
     4141 aggtcgtatt acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt
     4201 ttgcagtgag cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4261 cacgacaaac atctacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4321 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg
     4381 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4441 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4501 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccatcttt
     4561 tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4621 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4681 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggagg tgtgaaagtt
     4741 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaggtaatga
     4801 ctc
//
LOCUS       DAZOZY010000259         4696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZOZY010000259 DAZOZY010000000
VERSION     DAZOZY010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.262"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHURQ_003940"
     CDS             complement(<1..737)
                     /locus_tag="ACHURQ_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR0825592.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHURQ_003941"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHURQ_003941"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR0825593.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHURQ_003942"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHURQ_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR0825594.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHURQ_003943"
     CDS             4397..4678
                     /locus_tag="ACHURQ_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR0825595.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1128 a         1170 c         1181 g         1217 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttta
//
LOCUS       DAZOZY010000260         4691 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZOZY010000260 DAZOZY010000000
VERSION     DAZOZY010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.263"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(209..3313)
                     /gene="acrF"
                     /locus_tag="ACHURQ_003944"
                     /pseudo
     CDS             complement(209..3313)
                     /gene="acrF"
                     /locus_tag="ACHURQ_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="ACHURQ_003945"
     CDS             complement(3325..4482)
                     /gene="acrE"
                     /locus_tag="ACHURQ_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR0825596.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         1197 a         1205 c         1139 g         1150 t
ORIGIN      
        1 tcttattaac gagctgaatg ccattttatc accggaggtt ataaatattg cggaatgaat
       61 aatgacaaat tatgtgaatt gtaaaaatag tgaagggagc gggaactata gaaagttttt
      121 aaagagtgag tagaagaata tattattaat ttttatgggt ttcactgaaa ataataaagg
      181 cacccgaaag tgcctttatg tttctgattt atcctttaaa gcaacggcgg atcaccacaa
      241 agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct cccattaccc
      301 cgatacccac tgcgttctgc gcgccactgc cggcaccgtt actgatagct agcggtaata
      361 cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata cgtactgcca
      421 tcagtgtcgc ttcaacaaca cctttaccct ttttctccat gagatcttta gcgaactcaa
      481 caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc accataaagt
      541 agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca atccctaacg
      601 gcacgaccaa cataaccgag acaggaattg accagctttc atagagtgca gcaaggcaca
      661 ggaagacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc gataagcgtt
      721 cctgatacga catacctgtc cagtcataac caatgcccgc aggtaatttt gacgcaaggt
      781 tttccatcaa tgccatggca tcgccggaac tggttcctgg cgcggcttcc ccctgaatct
      841 ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc caatgtgaag
      901 tggtaaaggc agagaatggc accatttcgc cgttggcgct gcggacataa agtttatcga
      961 catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt ttcacgcgac
     1021 cacggtcgat gaagtcgtta acgtaagtcc cacccagcgc cgttgaaatg gtctgattga
     1081 tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc agtttaaact
     1141 gcgcggtgtc ttccagacca ttaggacgca cgctgactaa gctggcagga tgttgcgccg
     1201 ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc ccagcctgat
     1261 caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct ggcatattga
     1321 atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga tggattaccg
     1381 cttccgcact gttttcgtca ccactacgct cttcccacgg tttcagactg acgaaagcca
     1441 taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag acactttcta
     1501 cgttcgcttt ctcgttattc agatagtaat ccgtcacttg atccaacact ttttgcgtcc
     1561 gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc tgatcctctt
     1621 caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca acaatcagcg
     1681 catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg ttggtgtagt
     1741 ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccgccc ttattttcgt
     1801 gatgctcagc agagacgggt ttaagcagcg ttgcacataa cgcaggggta agaatcaatg
     1861 ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg cgataaattg
     1921 ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc accatcgcga
     1981 tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct tttggcggga
     2041 gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca tcatcgacga
     2101 gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag taaccaaaag
     2161 cggcgaggat ggcaaacgtc cctaacaaca ccacgggtac cgcaatggtg gggatcagcg
     2221 ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata atggcttcga
     2281 acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg tcataagggt
     2341 agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt gccttaatgg
     2401 ctttcgcggt atcgagagcg ttcgcgccgg ttgctagctt aatccccagg cccgccgccg
     2461 gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca actcgtgcga
     2521 catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact ttgccgaatt
     2581 cttccggatt ttttaaccgc gtctgagcaa taatcgaggc gttcaactgt tgccctggta
     2641 acgctggcgt tccgcccaac tgcccggcag cgatctgatc gttctgtacc ttcaactggt
     2701 taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc agccagatac
     2761 gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga cggctaagcg
     2821 tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg tctgggttat
     2881 cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca ctgatcccct
     2941 gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc tgcacctgca
     3001 cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc acgctaccgg
     3061 cggaatcgct ggtggaggac atatacatca ggttatcgat accattcata ttctgttcga
     3121 taacctgcgt caccgtatcc tgcacggtct gcgcatcagc acctggatag tttgcctaaa
     3181 cagaaaccgc aggcggtgca atggttggat actgagcgac gggcaattgt aggatcgcca
     3241 gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt cgtcgaataa
     3301 aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg tatcggtagt
     3361 ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga cttgatcgcc
     3421 agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg caacgacagg
     3481 gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac cacgcggtgt
     3541 gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt catcaatccg
     3601 tgcacgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga cagcacgtag
     3661 ggttatggag ccggtgcatt catcaacggt cacatcagag aattgcagcg tacctttcag
     3721 gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt tttccttatg
     3781 caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg attgggtcac
     3841 atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt gcccattagt
     3901 gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg gcgcagtcac
     3961 tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg caatcacggc
     4021 ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc tgatgtattt
     4081 cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg cggcggcttc
     4141 acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg cgggatcgat
     4201 ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac gattcagtac
     4261 gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc ggcctggtaa
     4321 ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct gcggttcacc
     4381 gacgtgagct ttctcttctc ccttatcgtt acaaccgacg attaaagccg cggagatcag
     4441 aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc ctcaaaaaaa
     4501 caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga aaaatacagt
     4561 ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata ctggcgtctc
     4621 aaggcacata aacacaaaaa aagattaata ttctactgtt ttattttgac gcgggttgaa
     4681 agaggcagaa t
//
LOCUS       DAZOZY010000261         4568 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZOZY010000261 DAZOZY010000000
VERSION     DAZOZY010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.264"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHURQ_003946"
     CDS             <1..461
                     /locus_tag="ACHURQ_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR0825597.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHURQ_003947"
     CDS             complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHURQ_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR0825598.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2231..4492)
                     /locus_tag="ACHURQ_003948"
     CDS             complement(2231..4492)
                     /locus_tag="ACHURQ_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR0825599.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
BASE COUNT         1166 a         1056 c         1055 g         1291 t
ORIGIN      
        1 aacaaaatgg gttggctaaa gctctgagag agattggccg tatagaacga acactgttta
       61 tgctggactg gtttcgggat ccggcattgc ggcgacgtgt ccaggccggg ctgaacaaag
      121 gagaagcccg taatgctctg gcccgtgctg tattcatgca tcgattgggg gagattagag
      181 atcggaaacc ggaaaatcaa agttatcgcg ccagtggatt gactttattg acggcggcta
      241 tttccttatg gaacacagtt tacatggaaa gagcggttga tgctctgaag cgtaagggca
      301 tgaaaataaa tgctcaacta ttatcgcatt tatcaccatt aggttgggaa catatcaatc
      361 tgacaggcga ttatatctgg aaaaacagcc ggataccgac ttcaggtaag tttcgtcgtt
      421 tgagatcagt taagatcgac aaggtcaaaa aacaacctta acgtacaata attttcgata
      481 tccaaactga ccccaagtat aaaaaacagc ctgtcacggt ataaaaatcg accatgcaga
      541 aattatgctg gttattttac cgctgctggt ccctcattca gtgactgccc caggggtttt
      601 catgctctcc ttatagtaca ataatttcca tatccaaact cacccctact ttgactttta
      661 tcatagtcaa aacctcttaa cgtgagtttt cgttccactg agcgtcagcc ctctaaaact
      721 actgaataat cagttagaaa acaaacaatc ggaaaaatct ggggttccgg cctagaaccc
      781 catgaacagg catagctgtg atttcattta attattggca caaatcagtt gatcgtacaa
      841 tgctgtgcct gtctcgtttt ctggagattc ctcagcctca tggagcggcg taacaggcgt
      901 accggcagga cagagatgtt tgcttttcct agtccggcca gtttagtgga ataacggagt
      961 ttttatccca tgttttttcc ccccgatcac gactttttca aatcaatgct cagaatacgt
     1021 tctttagctc cttccacaaa cacatcagga tcccaatcgc ggttaaagcc ttctttgatg
     1081 ccaatgtaaa gggtatccat atcgtccgac aacaccatct ggtttgggta gaccggcatg
     1141 ttaaattcac gggtgatgct gtatttggtg gcatccacta cgctgatggt gccgctagtg
     1201 aagttggtga catacagttc gttatatttc ggattgtatt tcaccgcgtt agctccggct
     1261 cctgtgtgca catagccaag ctgtttaccg tcgcgtaagt caaaaatata gagtccatct
     1321 tttgtaccgg aacgatcgtc atggtcgaat accaccacaa aggcacggtc agaagcggag
     1381 tccaccgcca gattctgcac acttgtgatt tgtggtctcg gatctttcaa cgggatggta
     1441 cgcagcagcg tctgattttt cgcatcaatt tcgttgatgt aatctcttcc gccaacgtag
     1501 attttctgtg ttttttcatc catcgtcaga ccatacgctg tacctttgta agcatcctta
     1561 acctcgcctt ttaaggcaag cgtcgtcccg tcgagcatta acagcttatg cagaggcttc
     1621 atgccctcgg ccgttttcag catattgcta taactcacaa acagggtatc ggaatcctgg
     1681 ctgtaaacca tatggcgaaa atgctcaaaa cgcgaatctg cagcgtttgc cgctttaaaa
     1741 cttaacctgt cgctggttct taccagtttg ccggttgggg tgtcaaacat actgatacgc
     1801 agggacgctg actgggtgtg tccgatatag agctgatgtt tatcctgatt tatcgccagc
     1861 gaaaatgccc ggtatggcat cgtgtattta ttttcgactt tcagcgaatc tgaattaaac
     1921 gaatacagat agcctttatt ggcttcacga tttacacgat cggtgactgc tgcaaacacc
     1981 tttttactgg cattatcaaa cgccagttca tagacgcctt catcagggga ctgaaggtaa
     2041 ttcgttttca gggcaaatcg cgaaagttcg cgttcaggtg gatcgatagc catggccatc
     2101 gtgggtaaaa atagtgaaat taacaggtat agcggtgctt tggataaagt gaaaatattc
     2161 atttttcgtt cctgcaataa aattgacata agagagaaaa cgtattgtct gaacctagcg
     2221 gaaattgtca tcagaaacga atgtccccgc caaatatcca tgttctgcca cgcgcggaat
     2281 tggctgggtg ggtctcgtca ccaagacctg gcatcatgtt gagcttattc agcgcctccg
     2341 aataatctct gttcatcagg ttttgtaccg aaagtttcag tgttaggttg cggttgtact
     2401 cgtaagtacc atagagatcg ataatcgttg ggatctgcgg caaatcctgt ttgattattg
     2461 cgccggtatg ttcgtcctgc tcaaaatcag gcgacaggcg acgagccttg cctgtgtatt
     2521 ttatgatagt gcccagggtc aacgcgttat cgaagaagcg aacaccagta tccagcgtca
     2581 tgtattttct gggcagttcg gtcatatccc ctgcgccaaa gtaggtgctg gcgatggagg
     2641 ttggctggtc tgtttgctgc tggctgaaag agagtcggcc aaaagcaaaa cctgcatcat
     2701 aatccatctc cagttcgaag ccctttgcga taacgtcgct tgccgagtta acgtagatgt
     2761 acatattgtc gctgtattca tcgctaatgc cctcccagtc attgccaatc acctcaggca
     2821 tactgcattt acgacctcca gaacaaacca gataagactc gctgtagatg tagttctgga
     2881 tcctgctgcg gtacgccaga gccttaaagc gcagggcatc ctgttcgacc agtaaatctc
     2941 tggtatcaat gttaaaaccc gcctgccagg tttctgcacg ttcaggcttc aggaatgggt
     3001 tcatggatgc gcctcctgaa ttagagaaga acatctcctg gatgttcggg gcgcgcatgg
     3061 atttgctgta gccaatgaac ggctgaagcc atggtgttac ctgagcagaa agctgaactg
     3121 aagggttgaa gccaccctct ttatcgtcaa tatcgtagct accctgtggc acgcagataa
     3181 cgcgtgaatc gcaggcgggc ttgtaccctg tgatcctgtt acgtgtgtag ttgagattga
     3241 aatctgcctc ccagatgccg cgcgtaacct tcagcccggt atacagcgct gaaatatcct
     3301 gctggccgga gggtgcgaac ggattgttct cgatcgattc ctgattcgct ttcgggtcgc
     3361 ccgccgctga gtgaatggtc cggtcatagc gggtacgcat cagtttgctg cccagcataa
     3421 actccaggtc attgtccgcg acagtgaacc ggctggtatt gttgatatcc agcgcgtcag
     3481 aacgattttg cgctgaggtt ttgtagaaag tatacagcga gccatcacga tacttttgat
     3541 taccgcgact ggtactggcc gttacgttaa agtcaattaa ttcagaaaaa ggggtgtaat
     3601 gatatttaat gtaatagtcg tcactggtta tatcacgacg tgtaaattta ttttcatagg
     3661 ttcgagcgga aagttcgaag ctgttaaatt cgtctggtct gatatccatt ttatacagtt
     3721 gggatttggg gttctgcttc atatatttat cataaccaaa ctctttgctg ttaattccag
     3781 aaccatttga gaaattagag tacacagaac tgccgctcac agcagccata acgccaatgc
     3841 ttcccgtatc cgtaaaagcg tcgctttttc caccaagggc gatcattccg ctgcgtccca
     3901 gcccattact accgacagag aaacgtgaac gaaggccata tgtattaccg ttaaatatta
     3961 catcgtcaac gccaatagta cgcatatttg cactaccggc aagggcgttg atcccttcag
     4021 agccactgct gtcaccgcgt gtaacatcaa ctgctaccag taagttagga tctataagta
     4081 cgccagccat attgttagtt gaaccatgcg tcgttgttcc ggaggtagag gttccgtaaa
     4141 aactctgggt aataccatcg accatagtgt ttacacgacc aaatccgctc atgcctcgaa
     4201 tattcacact gattgctccc tgaccaggat ctatttgagt ataagtacca ggcatactac
     4261 gcactgttgc gtcaagtgat tgcaggtttt tgtccgttgt ccgggaactg gtcgcgcctg
     4321 ttttctcgag agcttcctgc tcactggtcg tcttcccgac agaaacgctg agcggactga
     4381 attctgccgc tccttttttg tcggaattag ttttttcact gatggattgt gagtaagcac
     4441 cgcagctgag caataatatg cctgagccga tagccagttt tataacgttc ataaatttcc
     4501 ttgggtataa tattaataaa gataagtata actatttgtt tattaattat gttctactgt
     4561 ctttttga
//
LOCUS       DAZOZY010000262         4481 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZOZY010000262 DAZOZY010000000
VERSION     DAZOZY010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.265"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(8..1069)
                     /locus_tag="ACHURQ_003949"
     CDS             complement(8..1069)
                     /locus_tag="ACHURQ_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR0825600.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1066..2463)
                     /locus_tag="ACHURQ_003950"
     CDS             complement(1066..2463)
                     /locus_tag="ACHURQ_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR0825601.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2618..3616
                     /locus_tag="ACHURQ_003951"
     CDS             2618..3616
                     /locus_tag="ACHURQ_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0825602.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHURQ_003952"
     CDS             complement(3727..>4481)
                     /gene="ompG"
                     /locus_tag="ACHURQ_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR0825603.1"
                     /translation="EPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVH
                     YQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWL
                     SMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFST
                     QEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGL
                     SVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1227 c         1099 g         1063 t
ORIGIN      
        1 tattgcatta tttttcgctg ggtgttttgc ctttttgcag tgtttctttc acctgaccga
       61 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
      121 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtcctgca ccaatcccct
      181 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
      241 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
      301 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
      361 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
      421 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
      481 gctggaccaa atgcgcatac aggctgacca cttcacggtc gttttgcgtt tcatggattg
      541 aggcatacca gcgttgcagc gccggatgcg actgatcaat acccgcctgc tgcgccagtt
      601 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctatg caataaatca cgcgcttcgt
      661 cgcgttcatg ggcgcgctgt cgcaagcacc ataagcactg ccactctgtt accacagaac
      721 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgct acccagtcct
      781 gggtttgcca ggcattcatt gtccactgta caccctgccc gacaacactt gcgccaaaca
      841 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
      901 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
      961 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     1021 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     1081 tcctattaaa aattccagcg cggcatcaag acgaatatgc ggcagcggtt tgtcgacatc
     1141 catcacctgc gggcgaaacg cttcaaactg gaacccttgc ttgtcccaga acgcctgacc
     1201 gggcaaacgc gcaggaactt caccggggta aacggtgaga ggcgcaccat cgctgagccg
     1261 attaccgcgc aatgccggaa ttttctcgcc gttgacatca ataatgccgc tggtggtcgc
     1321 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     1381 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgaccgttac
     1441 atggtccgct ttcgttgcgg caaacagcag tttgtcgata accggcgaga ataagcggcg
     1501 aaagagcgtg cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     1561 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatccacca gcacaatctg
     1621 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     1681 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     1741 ctcgccccag gtatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     1801 atctcctggc aagacaaagc gccctggctg aataaagtgc agcccctgct gtttacagtg
     1861 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggtgc
     1921 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
     1981 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
     2041 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcttt
     2101 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
     2161 gccgcgcgta ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
     2221 ataggtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
     2281 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
     2341 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
     2401 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
     2461 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
     2521 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
     2581 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
     2641 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
     2701 tatctccccg gaagcgcgcg aaaaagtgtt acgggccatt gaagaattac agtatcagcc
     2761 aaacaagcta gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
     2821 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
     2881 cgccaaagct gaagaagaag gtttcgacgt gatattgcag acgtcgcaca acccggcaga
     2941 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
     3001 aagttcgcca gcggatgagt cattttttgc ccaactcgat aaatacgata ttccggtggt
     3061 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
     3121 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
     3181 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
     3241 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggtg gttataccca
     3301 tgaaacagca ctgcaagccg cgagagaatt attaagccag tcaccgttgc ctgaggcggt
     3361 gtttgccact gacagtctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
     3421 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatatt
     3481 aacgcctgca ccgggcggca tcgatgttcc gacgcaggag ttaggtcaac aaagctgcga
     3541 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
     3601 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
     3661 cttatccggc ctacatttat agatcggata aagtactcgc gccgcatccg gcaatttagc
     3721 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cactgtctcc
     3781 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagtc cgttctggaa
     3841 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
     3901 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
     3961 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtggaaaact caccgttatt
     4021 gcggctgtcg tccatattga agccgcgctc gagataatag ttcactcgca aggcaaccgt
     4081 ttcgttgaag gtatattgca gacctgtttc cgtttcgaca cgggtatcag cgtaaccggt
     4141 agtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
     4201 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
     4261 acccggttca tcaacgtagt gataaccata attacggaaa ccgccggtca ggccgaaact
     4321 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
     4381 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
     4441 ccacggcccg ttggcggcat taaaatagac tgaaggctcc g
//
LOCUS       DAZOZY010000263         4411 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZOZY010000263 DAZOZY010000000
VERSION     DAZOZY010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.266"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            97..1179
                     /locus_tag="ACHURQ_003953"
     CDS             97..1179
                     /locus_tag="ACHURQ_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFR0825604.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
     gene            complement(1368..1751)
                     /gene="quuD"
                     /locus_tag="ACHURQ_003954"
     CDS             complement(1368..1751)
                     /gene="quuD"
                     /locus_tag="ACHURQ_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR0825605.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1837..1977)
                     /locus_tag="ACHURQ_003955"
     CDS             complement(1837..1977)
                     /locus_tag="ACHURQ_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR0825606.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1974..2336)
                     /gene="rusA"
                     /locus_tag="ACHURQ_003956"
     CDS             complement(1974..2336)
                     /gene="rusA"
                     /locus_tag="ACHURQ_003956"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR0825607.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2333..2623)
                     /locus_tag="ACHURQ_003957"
     CDS             complement(2333..2623)
                     /locus_tag="ACHURQ_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0825608.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2616..2786)
                     /locus_tag="ACHURQ_003958"
     CDS             complement(2616..2786)
                     /locus_tag="ACHURQ_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR0825609.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2786..3241)
                     /locus_tag="ACHURQ_003959"
     CDS             complement(2786..3241)
                     /locus_tag="ACHURQ_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR0825610.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            3506..3883
                     /locus_tag="ACHURQ_003960"
     CDS             3506..3883
                     /locus_tag="ACHURQ_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825611.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTKLGEAITSSGQRVFGGDLSNVNFYGTNTIFDGDTIFLIKSP
                     DSEEWSSQKASKDVEGIVFGGKK"
     gene            3880..4308
                     /locus_tag="ACHURQ_003961"
     CDS             3880..4308
                     /locus_tag="ACHURQ_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825612.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT         1229 a         1000 c          942 g         1240 t
ORIGIN      
        1 ccaaaactta catcttgaaa taatcacatt gattagatga atatttatcg cgcagtgaca
       61 tcatttttta ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt
      121 tctgctgtag ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat
      181 aaagacagta acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct
      241 aatgatgcag atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa
      301 atcaacgatc aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct
      361 gaatctcaag gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt
      421 gactacggct ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg
      481 actgacgtcc tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact
      541 ggtcgcacca ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt
      601 ctgaactttg ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat
      661 ggtgatggtt tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc
      721 tatgctaaat ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca
      781 tctggtcaaa atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat
      841 ctggctacca cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc
      901 aacaaagcac aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg
      961 tccgttgctt acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg
     1021 gttgaatata ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt
     1081 gattacaaaa tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc
     1141 gatgatatcg ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt
     1201 tgcgggaggc tttgcctccc caacatataa gtggctccct caagccactt cctttaggag
     1261 cacaaccttg cttctaacta tataaacctt ctgttatata ttacccttta tttttggggg
     1321 cgttgcaacg ccccattttt aataattttt agtaaacaat tggcatatta attagagtta
     1381 ttaacaacga tatccatctc taaccggata tctaatgcca ttaacatccc ttcaattatg
     1441 ccctcagcct tctgtaacct tttcccgata taaccatcag agcagcaatg cttacctgcc
     1501 agtgacatga atgtcatacc gactacataa taatctacta ataaatcgtg caaatcgctg
     1561 ttgttctttt tcagacgggc catgcacccg caaataatca tcgcgtcatc gtcacaacat
     1621 tgcgggcgag attttacttt tgaagtaatt aatcccttaa aaccggcggc aatggacgac
     1681 caggtcacat cttcatgatt attagccgcc cacgctcccc aacgctcaag aaccatctga
     1741 atatcacgca tcaacttact ccacaaaaat cagaccagaa cgccaattac aagcaaaaat
     1801 caacaaaaca gtattagttg attgttatct ctgacttcat actcctgctc ctgtcagggt
     1861 tttggcgtaa ttcttcagta ttcggtaatc ggtcaaaaca gaaccgggga aacgatataa
     1921 gcgcagatgc ccccagcggt ggcgaagaag ttctgccata taaaactcaa acatcattca
     1981 ttccccattt cggtgatggt cagttccagc ctcccacctt tggtaacagg catcttcaca
     2041 acgcggtaat caacgacctg agcatcatcc agccagaaac ctgctttggt gagtgcgtca
     2101 aaagcggctt tttgcagatt atccaggtca cggcgacggc gatccggcat gtggcactca
     2161 atacggattt tcactggcat agccaggccg atatccagca ttgcgttttt aatgattcgg
     2221 gtgacgttat cgcggtatgc ctgcccttct gcgctgatgt gtgtgcgccc gcgattatgg
     2281 cggtaatagc ggttattgct cggaggccag ggtaatgtga tgctgtaggt attcacgcct
     2341 taataacccc ctctttcagc cagataacct gcgttctcgc cataccttcc agcgcgcatt
     2401 cttttgcata ctcagcatcg acaaaatatg tgcggcggtc gatttcgtcg tgacaggcag
     2461 aacatgcaat ggtggcaatc aggtctggcg gtttgatacc ggtaccgcac aatccagcca
     2521 gccggatatg tgccagtact gacgtttcag gattgccatt acatacgcca gggattctta
     2581 cctggcattc ccgaccacgc gctgcttttc tcaaatcagc catgattcct ccttgctgcc
     2641 agtcgcaacc attttttatc aaccaggcta gcggtatatc cgagcagtgt tggtatttcg
     2701 gatggcttca gctcaggctt acgcttacga cgatttgata ctctgtagat gtgcccgttc
     2761 atgacacgaa taagcggtgt agccattacg cctcctgctt atcgcgcagc tgctggtatt
     2821 cacagctctg cggaattgtc aggtggcagc cgatattcat cgcccaggct tcgactttgc
     2881 acaggaagat gtacatctcg ccggtttcca gctctgacgt atggcggagg gattggacgg
     2941 tggtgacctc gccggacacg acatctacac ggtctttgct ttcgtagccg agataggtgt
     3001 gcttcatcgc gtctttgacc cactcaggcg tagcgaaggt cttgccgcgg gcgatgagat
     3061 attcgctgat ctccgtgtac cacatgtggc tgagcgcgtt ctgcgacagg ctgcgcttct
     3121 cgcgccacgg cttaacctgc aggcgtaagc attgcccggc atccagcaat ggctgaatct
     3181 gctggccgat ggccgcgaag ttaccgcgat ggagtttgat gccgtctact ggcagagtca
     3241 tacggcctcc ctaacggaaa ccgcagaatg cagaaaatcg caggtgcatt tctgcatctg
     3301 tgacaaggtg atgagttcag attgtggtcg catttaagtc cccttaaatg cgcagaagtc
     3361 accggagttg ttcaggctcc gatgagagga ttatggcgga ttgattacga taaatcaaaa
     3421 ctatcttgaa gccaaaacag tgatcttcgc tttaaaaacc ttgtatactt tcacctaatc
     3481 acagccagaa ctatggatct aaccaatgac caacaaagaa aacaaagata aatatattaa
     3541 caagttcaag aaagagcttt caaaatatat acaaccaact gctggagtgg acattcaatt
     3601 atttaactct aaggatggtg gtggtgtaat taaagccact ttgaatagaa gtggaaaaag
     3661 aaaatcatct attgctggta attttactaa attaggtgag gcaattacct cgagtgggca
     3721 gagggttttt ggtggtgatc tatcaaatgt gaatttttac ggaacaaata cgatttttga
     3781 tggagataca atatttttaa ttaaatcacc tgattcagag gagtggtcat cacaaaaagc
     3841 ttccaaagac gtagagggca tcgtatttgg agggaaaaaa tgattaacat tactactaag
     3901 gatggcactc aagtcagtgg tgaagtaaga gatctagtaa ttcttcttga aactgatgga
     3961 aaaacggtgt ctgattattt gcagccaatc cctcccaaaa aagatacaca ctggagcatg
     4021 attttcattc cggtagcaat ttatatacta cttaatcttg tatacattat tgctcaccca
     4081 ttatttcctg aaaaatatct aaaaacatca acatctatat ttctattcat ttccttcata
     4141 ttaagtgcaa tagtagcata ttttgttttc cataagcata aatctgccac tctatccata
     4201 tgcagcttag tgttcttagt gttaattgta ggtgctaacg tagggtttat cagttacagc
     4261 gacctaacac aaaaagcgac gcaaaaatta gatagtttgt ctaaataatt atgttgccat
     4321 gcgaagtatg catggctgca tgtctgcctt ccatttaaaa tgggctaaga cctataaccc
     4381 taaatattat tctttattat cttctttacc a
//
LOCUS       DAZOZY010000264         4410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZOZY010000264 DAZOZY010000000
VERSION     DAZOZY010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.267"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHURQ_003962"
     CDS             complement(<1..105)
                     /locus_tag="ACHURQ_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825613.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHURQ_003963"
     CDS             complement(173..2122)
                     /locus_tag="ACHURQ_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR0825614.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHURQ_003964"
     CDS             complement(2207..2542)
                     /locus_tag="ACHURQ_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR0825615.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHURQ_003965"
     CDS             complement(2542..2898)
                     /locus_tag="ACHURQ_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR0825616.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHURQ_003966"
     CDS             complement(2898..4394)
                     /locus_tag="ACHURQ_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR0825617.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagccttat
//
LOCUS       DAZOZY010000265         4395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZOZY010000265 DAZOZY010000000
VERSION     DAZOZY010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.268"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1343
                     /gene="xanP"
                     /locus_tag="ACHURQ_003967"
     CDS             <1..1343
                     /gene="xanP"
                     /locus_tag="ACHURQ_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR0825618.1"
                     /translation="QNSELIYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQAL
                     GLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALK
                     TGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVG
                     LTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYAL
                     AWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATS
                     DVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYV
                     GFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAI
                     LIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1464..3173
                     /locus_tag="ACHURQ_003968"
     CDS             1464..3173
                     /locus_tag="ACHURQ_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR0825619.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(3225..>4395)
                     /gene="yicI"
                     /locus_tag="ACHURQ_003969"
     CDS             complement(3225..>4395)
                     /gene="yicI"
                     /locus_tag="ACHURQ_003969"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0825620.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT          974 a         1204 c         1177 g         1040 t
ORIGIN      
        1 ctcaaaacag cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt
       61 ttgccgcctg tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa
      121 tctgccaggc gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt
      181 ttgcctccgg tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc
      241 tgttgtctat tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg
      301 cgctgaaaac cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga
      361 tgctggcaag ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta
      421 ttacgccgct ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg
      481 ggctgacctc cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga
      541 aaaatctgct gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta
      601 acccttactt acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt
      661 ggtttatggg catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc
      721 caacgccgct ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg
      781 tctttatgat cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg
      841 aacagccagt gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg
      901 gcctgaactc gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa
      961 acaacggagt gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc
     1021 tgatgttgat cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag
     1081 aaccggttct gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc
     1141 gtatcgtttc tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg
     1201 cggttggtct gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga
     1261 aaaacctgct ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga
     1321 ttttcccacc agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct
     1381 cacctcttta accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg
     1441 tgcatttcac gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca
     1501 tcgctctgtt agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag
     1561 aacatatcag cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg
     1621 ttcaccgttt ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg
     1681 gccagcccgc gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga
     1741 ccgaaccacg ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc
     1801 agaccgcgcc gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata
     1861 gcccgaatag cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt
     1921 caccagaagc cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc
     1981 tttcgctcaa tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc
     2041 gcgttacgct gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc
     2101 agcgtaacgc cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac
     2161 aaagcgaaaa atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga
     2221 ttggtcgcct ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc
     2281 tcgatctcag cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca
     2341 aactgtcgat gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta
     2401 taaacgcgga attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag
     2461 ggggtatggt cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg
     2521 atgcggcaat tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg
     2581 aaacgacacc cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc
     2641 tgatcattga tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg
     2701 ccaacctgac gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga
     2761 atgccgccgc cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg
     2821 ccaacagcag cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg
     2881 aagccaccgc cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac
     2941 gcggtgtgcc ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg
     3001 acggcaatat tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta
     3061 cggttagcgg gcaattgcat gccgtgaacg ccgcaaagca ccaagtgact caaaccatga
     3121 atgcgggcgt cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa
     3181 tctcgccggg tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc
     3241 agcgcattcc cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct
     3301 tgcagaccat ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc
     3361 gcaccagtca cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa
     3421 ccgtcagcag cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg
     3481 aatgccgtgc cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat
     3541 agggtgttat cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag
     3601 cgactaccgt cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag
     3661 aactgcacct cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg
     3721 tattgacggt caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc
     3781 atcatcggcg taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg
     3841 cggcatttca gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac
     3901 ggcacacgat aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg
     3961 caccagcgtt tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc
     4021 cagaagccaa aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat
     4081 tcgtagttag cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag
     4141 gccgagcggg caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac
     4201 accagttcgt tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca
     4261 aaccactgaa catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc
     4321 atcgcgacca gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag
     4381 tcataaatcg ccaga
//
LOCUS       DAZOZY010000266         4364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZOZY010000266 DAZOZY010000000
VERSION     DAZOZY010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.269"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHURQ_003970"
     CDS             complement(<1..215)
                     /locus_tag="ACHURQ_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0825621.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHURQ_003971"
     tRNA            complement(330..405)
                     /locus_tag="ACHURQ_003971"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHURQ_003972"
     tRNA            complement(412..486)
                     /locus_tag="ACHURQ_003972"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHURQ_003973"
     tRNA            complement(603..687)
                     /locus_tag="ACHURQ_003973"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHURQ_003974"
     tRNA            complement(696..771)
                     /locus_tag="ACHURQ_003974"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHURQ_003975"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHURQ_003975"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR0825622.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHURQ_003976"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHURQ_003976"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR0825623.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHURQ_003977"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHURQ_003977"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR0825624.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHURQ_003978"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHURQ_003978"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAZOZY010000267         4330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZOZY010000267 DAZOZY010000000
VERSION     DAZOZY010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.270"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..583)
                     /locus_tag="ACHURQ_003979"
     CDS             complement(<1..583)
                     /locus_tag="ACHURQ_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR0825625.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(646..1095)
                     /locus_tag="ACHURQ_003980"
     CDS             complement(646..1095)
                     /locus_tag="ACHURQ_003980"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR0825626.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHURQ_003981"
                     /pseudo
     CDS             complement(1092..4184)
                     /gene="ebgA"
                     /locus_tag="ACHURQ_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1044 t
ORIGIN      
        1 tgaagttcgg gatcatcgcg tcaacggtga tggcgtcagc aggctgtacg ccgccaacca
       61 gcgccgtacc tgccagtaaa atgtaggaga gcgttaacag cagcatcagc gttgaagtga
      121 cggaggtaat tggccccaac attttcgccc cgttggtgga aacccaggtg gagaaggcga
      181 acagcaccat actgataatg gtggtggcaa ccggcgtcat aatatattcg tatccgagga
      241 aggcatacga agcataagca ataacgcgcg gcaacagtga ggtgaaaaag aacaggttta
      301 cgaaccagta ggtataggca gtaataaatg cccaacgtcc gcccagcgaa ctttttaccc
      361 atgcgtagac accggcttct gagtttttat ttaacgaaac aaattctgcg atgatcagac
      421 agaagggaat aaaataaaaa atcgtcgcga ggaaaaacat cggtgccgag gccagtccaa
      481 gatcaatatt attattgata acgttgttaa agctgtaaac ggcggcaaaa gtcagcgaga
      541 gcaagccgaa tttgccgatt gtattacgtt tggtatcaga cataacacat ctccgaaaat
      601 gagtagggta gcggtggcgg caaactcctt ttgccgccgt agttgttatt tgttatggaa
      661 ataaccatct tcgatggtga ctttgagaac cacttttttg accgcgttat tgcagataaa
      721 ccgatacgct tcatggatat cgcaaataac gatttgccct tcgtggacct caacggtttc
      781 tccgcagcct tttaaatatt cacggtcagt ttcatcacga taatattcca ctacctgtaa
      841 tgtctctttc ggcgcatatt caattttttg ctgcccttgc aggtagtaat gcacttcaaa
      901 atagcgtcga tgcccggtaa atagcgcatc ggtcgcggag tctgtctcca cgcggtaagt
      961 caatgagtca ccgatggagt gggcgacgcc aggctgaatg ttgtcgatat tttcaatcgc
     1021 ttcaacgcag cgttgccact tcttgccaga ggcgtaaatc tggcggaact gttctaagtt
     1081 atcgatgatc ctcattgctt attctcgctg tgcaaattcg tggaaaagaa ccctgcgccg
     1141 aactcatacg acgccaggct ttgcgcggta gcttctccgc cagaaaccgg cagcaacgta
     1201 aagccgtagc tgaagtcacg gaaccagacg cgccaggagt ccagcacctc gctgccccag
     1261 gagttggagc cgaggccaag cagctggtga tcgaggttca gggtgatgtc atcactgcgc
     1321 tgcagctcgt tacagtgctg ggcagcgtgg atgttttcct aggtatagtg ccaggcgctg
     1381 aagttaattg ggcgctgcgg aaccaccagc agaccgttac cgtggcggtt agtcagtgcc
     1441 gtccagcgga catgctgacg gttaccgttg ttctgcggga agggatagtt ctcgaacatg
     1501 gcatcgacgg tgctgcgcca gatatcgatg atgttagcct gctggctgtc ggcgtagttt
     1561 tcgcccggtc cacgaccgta atacgccacc tgatcgtatt cgccgttaat tcccatggtg
     1621 aaaccgatgc acggaatgat gtgcggatag tcgccgtaac gctcgccgga aagcgccacg
     1681 ttaacctggc catcggcagc gatgcgccag atgtaggtgc agcgcattcc gaaatcaaac
     1741 accggcgggg caataaccgt gcggctgatg atcaacactt caccctcgct ctgttctacg
     1801 gcaaagtcgc gcagatgttc ctgcatgatc tgcaaatgat tcggctgcca caacccttcg
     1861 tactcctgct tgtggttgtc gatcatcggc ttgaagaagt tgatctttgg ctcgcgggtc
     1921 agcagcgact cgccatttac ctgccaggat gtcggtttgc cactcatttt tgagaaggtg
     1981 atcgcgaagt tgtagccgcg aacggtgcag ctcaaacgat cgtcttccag cgtcagcgga
     2041 cgcgcattat ttggtgcgaa aggcactggc tgcgcggtgt tttccttcag cgggaactga
     2101 taagtggcga tagaatgccc ggcttcgctg tagcgggtgc gggaatcttt ggtcaccgta
     2161 atgttgagga acgcttcgcg ggcgtccagc tgcggcagcg tgatctgcaa gggggcttcg
     2221 ctgttcggcg caacgtcgcg cagtttaatc tgctgcgtcg cgagcgtttc accttcggcg
     2281 cgcacctctg cgtgcagggt gtagtcatca agcgtggtaa accacagttt attttcgact
     2341 ttcaactcgc cgcgagtcag atcccgcgcg tggattttta ccggcgcgat aacctgtttg
     2401 tactctttca ggcccggtcc cggcgtctga tcggaataga tcaaaccatc aagacagaag
     2461 ttatagttgt tgggatagtc gccgtagtcg ccgccgaatt tataccagac attgccgtgg
     2521 tcgtcctgtg cctggatccc gtggtcgcac cactcccaga cataatgacc ctgaatgcaa
     2581 tcgtgcttat agaagacgtt ctggtactcc gtcagcccgc ccggtccgtt ccccatcgca
     2641 tgagcatatt cacagatgat gcgcggcttc ggatgcgggt attcaccaaa ctcattcatc
     2701 agcggcacgc gggtgtacat ggtggaaata atatcgacca cttcagcatc gcgatcttct
     2761 tcgtaatgca ccagtcgcgt gtcatccagc gccttcgctg cgtggtacat cgcgcggatg
     2821 ttacagccat agccggattc attgcccagc gaccagatga tgatcgacgg atggtttttc
     2881 tgcgcgtgaa tatggcgaac aatgcgctcg acgtagactt tttcccactg cggatcgtcg
     2941 gtaatacggc tgatatcgcc gacattagca aagccgtgcg attcgacgtc ggtttccgcc
     3001 atcacaaaca ggccgtagat atcacacagt tcgtaaaaac gcggatcgtt cgggtagtga
     3061 gcggtacgca cggagttgat gttgtgctgc ttcatcaact ggagatcttt ctcgacgcga
     3121 tccattccaa cggcgcggcc tttgcgatga tcgttgtcgt gacggttgac gccgtgcagc
     3181 atcacataac ggttattgat ccagaacaga ccgtcgcgca ctttgatatc acggaagcca
     3241 acgcgttgtg gtaccacttc cagaacgttg ccgttggcgt ctttcagcgt catgaccaga
     3301 tggtaaagat aaggggattc tgctgaccat tgctgcggct gttcgacagt aaaagcaaag
     3361 ctggcgctgg tcagtttttc aattgccaaa tgatcaatgg cgctgctgtg caccacgcgt
     3421 tcgccatcaa acagggtata ttccagcgtc gtgacgacag gggaggcggc gagattttcc
     3481 agcaccactt cgcaggaaag cgtggcatcg caataggctt cgtcaaagtc ggtacgcacg
     3541 gtgaaatcgt taatatgtgt tagttgtttt ccgatcagat aaacatcgcg gaagatcccc
     3601 gctgaccacc acatatcctg gtcttccacg taggtagagt ccgcccattg catcacgcgc
     3661 acacacaaca ggttgtcgcc ggttttaacc atcgcgctga tgtcaaactc tgcggtcagg
     3721 cgactgccct tgctgaaacc cacatactga ccgttaacgt agacttcaaa ataggtttcg
     3781 acgccgtcaa atttaatcag cgtctgttta ccctgccagc cgtcgctgag ggtgaaaata
     3841 cgttgatagg caccggttgg gttatcgctg ggaacaaacg gcacatcgat ggggaacgga
     3901 aaaccttcgt cggtatattg cagtttgccg tgaccttcca tttgccacat ggcggggacg
     3961 gtaatatgcc cccagtcagc cattaactca gaggtgaagg cttcaggcac ttgcagcggg
     4021 tggtcaaaaa agtggaaatt ccactgaccg cttaagggca gaaacaggct gctggtttcg
     4081 cgggcaaagg tacgcgcttg cgcaacagaa tcatatgaaa aaaagtacgc acgtggcgca
     4141 agtcggtttt cgtgggtgag ctgaatgttt tcccagcgat tcatagggcc tcccggtaga
     4201 gagaaatcgg aaccattcat ggtgatggta gtttgtgtaa ttgctgggga gtttagtaaa
     4261 atttttacta aaaagtggca ggtaaatgca tttactttaa ctggatcacg aaaataaacg
     4321 gcagcactgt
//
LOCUS       DAZOZY010000268         4309 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZOZY010000268 DAZOZY010000000
VERSION     DAZOZY010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.271"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHURQ_003982"
     CDS             complement(<1..1870)
                     /gene="speC"
                     /locus_tag="ACHURQ_003982"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR0825627.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDA"
     gene            complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHURQ_003983"
     CDS             complement(2033..2116)
                     /gene="yqgH"
                     /locus_tag="ACHURQ_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR0825628.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2269..2976
                     /locus_tag="ACHURQ_003984"
     CDS             2269..2976
                     /locus_tag="ACHURQ_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR0825629.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3082..3157
                     /locus_tag="ACHURQ_003985"
     tRNA            3082..3157
                     /locus_tag="ACHURQ_003985"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Phe,seq:gaa)
     gene            3355..>4309
                     /locus_tag="ACHURQ_003986"
     CDS             3355..>4309
                     /locus_tag="ACHURQ_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0825630.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1114 a         1141 c         1037 g         1017 t
ORIGIN      
        1 gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac atcgcttttt
       61 gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac aactggcgca
      121 gggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac acctcagcca
      181 gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc agttgtgcca
      241 gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga tcgcacttct
      301 ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga acgccaaagt
      361 cgctatattc gccggtttcg gcattgatac ctggtgtagt gagtaacagc ttgcacggat
      421 caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc cccggctcaa
      481 aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc cacaatttgc
      541 catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga atagccttgc
      601 gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc tccccttcat
      661 gaattttggc gttaacatcc agtgcagcaa acagcggata gaaagggctg gtagaagcat
      721 ggagcataaa ggcgttattc aaccgcttat gcgggcaaaa acgcgcctgt ccgcggatat
      781 ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt ttgtgcaccg
      841 actgagtcac aaagatcccc ggatcgtttt cgttaagttc taatagcagc ggcgagctat
      901 ccgccatcat cgggataaac tgttcgtaac cgacccacgc ggaatcaaac agaatgtaat
      961 cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtt ccgtcatagg
     1021 ttcccagctg aataatcgcc aggcgaaacg ggcgcggcag ctcggctttt tctggcgcaa
     1081 cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca ataccgccaa
     1141 tgaaaccaaa cgggttgcgt gaagcttcca gatagaccga cgtcgccccc gcctgaatca
     1201 gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga tcgccacgcg
     1261 ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc acaaaatagg
     1321 ttttatcggc atgaaagact ttggctgcga atttctgcgc atccttcgcc gatccttcat
     1381 gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga aagacgttct
     1441 caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac gcaccatgtt
     1501 gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc agcgtgtcat
     1561 aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc tccagccact
     1621 gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca gcatgttcgg
     1681 aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga atgccactgc
     1741 gactatccgc agcggtaatg acgactgccg cgacatccgt aaaatcagta tctcccaacg
     1801 ccaccacgcg gcgatgagta gaaagtcggg ataccagttc actactggcg gcaatattca
     1861 ttgatttcat aagcgcaaaa cccgtttcgg ggaagtaaga ataccggaca aagtgtaaaa
     1921 ccctgcccca tgagatatgg gtcaaactgg tcaccagctc cgaccgccag acatcagtaa
     1981 aagcagaaac gctctgattt tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca
     2041 tgagcagtaa catagaaagg gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta
     2101 aggagatggc gtgcatcggg caaactccgt acagagagga gaaaattcgc gcaatcatgg
     2161 cacctttcgc taaggcgtgc aagccaggac tttgcgacca gacaagccat caacaaggca
     2221 aaaagttcat aataagaaaa tcaaacaata cagctaacag gaactcttgt ggtcatcggc
     2281 ccttttatca acgctagtgc agtcttactg ggtggcgttc tcggcgcact gctcagccaa
     2341 cgcttaccgg aacgtatccg cgtctccatg acatctattt ttggtctggc atcgctgggg
     2401 attggtattt tactggttgt gaaatgcgcc aaccttccag cgatggtttt agctacgcta
     2461 cttggcgctc taattggcga aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc
     2521 aaagcacaaa atctgtttcg tcactcacgt aagaagccag cgcatgaatc ttttattcag
     2581 aattatgtcg cgattattgt cctgttttgc gccagcggca ccgggatctt cggggcgatg
     2641 aacgaaggga tgaccggcga tccgagtatt ttaatcgcca agtcatttct tgatttcttt
     2701 acggcgatga tcttcgcctg ctcgctgggt attgcggtgt cggtaattag tatcccatta
     2761 ctgatcatcc agctaacgct agcgtgggct gccgcgctga tattaccgct gaccacaccg
     2821 tcgatgatgg cagacttcag cgccgtaggc ggtttattgc tgctggcaac cggattacgc
     2881 atctgtggca ttaaaatgtt cccggtggtc aacatgcttc cagcactcct tctggcaatg
     2941 ccgctttccg ccgcctggac cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
     3001 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
     3061 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
     3121 ccccgtgtcc ttggttcgat tccgagtccg ggcaccaaat tcatatcaac ggacctccac
     3181 ggaggtccgt ttttcgtttc agaacgccac gatttaagcg ttctgcctac aaatcaattc
     3241 taccgaactc aaccagattc cccccacatc aaccccattg tgtgggtata attgcgggca
     3301 taccccagtt cgacagaatt tgtaccctct ttaacgcatt cagaaggctg aactatggca
     3361 ctgactgacg caaaaatccg ggctgcaaag cccactgaca aggcttataa actcactgac
     3421 ggggctggca tgttcctgct ggtacatcct aatggctccc gttactggcg tctccgttat
     3481 cgtattctgg gtaaggagaa gactctggca cttggtgtgt atccagaagt ttctctctcc
     3541 gaagctcgta caaaacggga tgaggcccga aaactgattt cggagggggt tgacccttgc
     3601 gaacagaaaa gagctaaaaa agtagtccct gatttacagc tctcttttga acatattgcg
     3661 cgacgctggc atgccagtaa taaacaatgg gcacaatcac acagcaataa agtactcaaa
     3721 agccttgaga ctcacgtttt cccctttatc ggcaaccggg atatcacaac actcaatacc
     3781 ccggatctgc ttatccctgt tcgtgctgca gaagctaaac aaatttatga aatcgccagt
     3841 cgtctgcagc aaagaatatc tgccgtaatg cgttatgccg tacagtctgg catcatcaga
     3901 tataatcctg ctctggatat ggctggcgca ttgactacgg taaaacgcca gcatcgcccc
     3961 gctcttgatc tttctcgcct gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa
     4021 cctgtcaccc agcttgccgt tatgctgaat ttactggttt ttattcgttc cagtgaactc
     4081 agatacgccc gctggtctga aattgatatt gacaatgcca tgtggactat tccagccgaa
     4141 cgcgaacccc tgccaggcgt aaaattctca caccggggct ccaagatgcg aacaccacat
     4201 cttgtgccac tcagcaaaca ggctgtagcc atactgacag aactacagac atgggctggt
     4261 gaaaatggtc tgatctttac gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAZOZY010000269         4289 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZOZY010000269 DAZOZY010000000
VERSION     DAZOZY010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.272"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            162..314
                     /gene="hokA"
                     /locus_tag="ACHURQ_003987"
     CDS             162..314
                     /gene="hokA"
                     /locus_tag="ACHURQ_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR0825631.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(291..362)
                     /locus_tag="ACHURQ_003988"
     CDS             complement(291..362)
                     /locus_tag="ACHURQ_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825632.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(502..714)
                     /gene="cspA"
                     /locus_tag="ACHURQ_003989"
     CDS             complement(502..714)
                     /gene="cspA"
                     /locus_tag="ACHURQ_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR0825633.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(995..1285)
                     /locus_tag="ACHURQ_003990"
     CDS             complement(995..1285)
                     /locus_tag="ACHURQ_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR0825634.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1719..2429
                     /locus_tag="ACHURQ_003991"
     CDS             1719..2429
                     /locus_tag="ACHURQ_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR0825635.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2479..3453)
                     /gene="ghrB"
                     /locus_tag="ACHURQ_003992"
     CDS             complement(2479..3453)
                     /gene="ghrB"
                     /locus_tag="ACHURQ_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR0825636.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3557..4216)
                     /locus_tag="ACHURQ_003993"
     CDS             complement(3557..4216)
                     /locus_tag="ACHURQ_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR0825637.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1062 a         1033 c         1048 g         1146 t
ORIGIN      
        1 tagaatagcc acgggtgctt gagactgttt gtctcaggta ttcaccgaaa ggcagacaga
       61 gaaaagcccc acctgactat aaatcaaagt gaggctaccc tatgcctgaa caccataagg
      121 ttagcctctt actcgttgga aatcaacaca ggaggctggg aatgccgcag aaatatagat
      181 tactttcttt aatagtgatt tgtttcacgc ttttattttt cacctggatg ataagagatt
      241 cactgtgtga attgcatatt aaacaggaga gttatgagct ggcggcattt ttagcctgca
      301 atttgaaaga gtaagagtct ttggcgggaa gttattcccg cctttcttac ggcgttgcgc
      361 attctcattg cacccaaatt tattcttcac aaaaataata ctaaatttta ttacgcgatc
      421 gattatttat ttcctgaaaa acaaataaaa aaatccccgc caaatggcag ggatcttaga
      481 ttctgtgctt ttaagcagag attacaggct ggttacgtta ccagctgccg ggcctttagc
      541 gccgctttcg atggtgaagg acactttctg accttcgtcc agagatttgt aaccatcgtt
      601 ctggatagca gagaagtgta cgaacacatc tttagagcca tcgtcaggag tgatgaagcc
      661 gaagcctttg tcagcgttga accattttac gataccagtc attttaccgg acatagtgta
      721 ttacctttaa taattaagtg tgcctttcgg cgatatggcg tgctttacag attttgaagc
      781 gttaaaggaa tgtgcactac gaggggtatc aacgataact cttgaaggga cttgccttac
      841 tacactgctt taatggtctg tacgtcaaac cgttgatgtg cattaagcca cgcattggcg
      901 ggtgatgcaa caattatttt tcatatttat gattaatcgg ttggaaaacg gtcctgtcat
      961 caggaccgta aacagcaata aagtggataa aagtctattc catcaactgc ttacttaatg
     1021 ccgggttggc ttgaatcaaa cgcatcaatt ttagttcggc acttgaaggc ttcacgcgtc
     1081 tggattccca ttcctttacc atggcgactg atacgcccaa aacccgggcg aaatcatcga
     1141 tttttaaccc tgtccctttt cgtaactgct caatttcggt acaggacgtt gttctgtgcg
     1201 tcagggtaat tttctgcgtt tcatctttaa aaacaatctg ttccaggctg ctcaacagct
     1261 catgcattgg atctttatat tccattgaaa actcctcaaa tcacactgcg ggatcgtgaa
     1321 ttgcatcgaa gctcattaag aatagtcggg aaaagcagac acagcggggt gaagtcaggg
     1381 catgctctgg ctatgattcg attcagcctg tcatttagcg tgacttatag ccacctaacg
     1441 atctgataaa tgccgatata tctcttacca ctcctgtttt gtaaagattt cgaaaatcta
     1501 accttctgcg aaggcgtaaa tctctggcta caaaaaaggc aacggcacta tcatttttta
     1561 acaatgaaac aacaaattaa ccttgtttca ttttctccat gattttacca ggaagattac
     1621 gtagaaaggg tatcctgcaa gcctgtcctg gactatcctt gtcacgtcag acacgcgtgt
     1681 gtcgttgtgc gctttttttg ggtgaaagga gtaagaaaat ggcgacagga aagtcctgct
     1741 ctcgctggtt tgcgcctctt gcggcgttat taatggtagt tagcctgagt gggtgttttg
     1801 ataaagaagg cgatcagcgt aaagcgttta tcgacttcct gcagaataca gtgatgcgta
     1861 gcggtgaacg tctaccaacc ctgactgccg atcagaaaaa acagtttggt ccttttgtct
     1921 ctgattacgc gattctgtat ggttattctc agcaggtgaa tcaggcgatg gattctggtc
     1981 tgcgcccggt cgtagacagc gttaacgcaa ttcgcgtacc gcaggattac gttacgcaaa
     2041 gcggtccgct gcgtgaaatg aatggttcgc tgggtgtgct ggcgcaacaa ctgcaaaatg
     2101 cgaagttgca ggccgatgct gcgcactctg cgctaaaaca gagcgatgac ctgaaaccgg
     2161 tatttgatca ggcattcacc aaagtggtaa cgacgccagc tgatgcattg caaccgttga
     2221 ttccggcggc acaaactttc acgcaacaac tggtgatggt tggagactac attgctcagc
     2281 aaggtactca ggtgagcttt gtcgcaaatg gcatccagtt cccgacttca cagcaggcaa
     2341 gtgaatataa caaactgatt gcgccattac cggcacaaca tcaggcgttt aatcaggcct
     2401 ggactacagc agttactgca acccaataaa gagtaaaagc ccgagcgatt atcgggcttt
     2461 acttcacgca gtcgcggctt agtccgcgac gtgaggattc acacagttct tctcaacctt
     2521 tccttgtaac gcatcaatta aattatccac ggcacaggcg gccatgccat aacgcgtctc
     2581 atgggtggca gatccaatat gcggtactgc gacgacgttg gccattgaga gcaacggcga
     2641 atctacggac agtggctctt gttcgaagac atcaagcccg gcggcgtgaa tttccccttt
     2701 ctgtaatgct gcgatcagtg cattttcgtc aaccaccggg ccacgtccgg cattaatgaa
     2761 aatggcggag gatttcattt tggcgaattg ttctgcgcca aacagatgat gcgtctcatc
     2821 agttaacggc aggatcaggc aaacgaaatc tgactcttgt aacagtgtat ccaaatcgca
     2881 gtagcgggcg ttgaagcgtt cttctgcttc tttatggtgg cggcgcgcgt tatagaggat
     2941 gggcatgttg aagccaaagt gcgcacgttg tgccagcgcc ataccgatcc gtcccatccc
     3001 gacaatgccc agtgttttat ggtgaacgtc agtgccgtac cagtccgggc ctatgctcgc
     3061 ggtccattcg cctgctttta cccgttctgc tacctccaca acccgacgag cggtagacaa
     3121 caccagcgcc atcagcgtat cggcgacggt ttctgttaat acggttggcg tgtgcatcag
     3181 cagaattttt cgggcggtaa gcgcatcgac atcaaaattg tcatagccga cggagatcgt
     3241 tgatgtggca tgcagtttcg gcattttttc cagcaatgcg gcatcaacat tctcgtttga
     3301 acccagtaaa ccttcagctt cggcaaaaat tgctgcattt tgttcgacgg tttgtgggct
     3361 gaggtttgcc acctggtgaa cggtgaaatg ctcttgcagg cgttgcagta aatcatcagg
     3421 taaggctttg tagaggataa cggacggctt catgcttctc tccattcact gataaggcga
     3481 tcaccttagc ctgaaatatg atgaaggaaa actgaagaat gccaggtgat ttctgcatca
     3541 cctggcatga aagggattac agcgggctta aggtaatttc tacacggcgg ttttgcgcct
     3601 taccttctgc ggtgctgttg ctggcgattg ggttagccgg gccaaggccc tgagtacgga
     3661 tgcggctggc gtccacgccc tgggtgatca acgcgctggc aacggaatcc gcacgctgct
     3721 gggagagacg catgttcagg tcgtgacccc ccgtgctgtc ggtataacca atcacgttaa
     3781 ccgccgtttt cggatactct gtcagcacca ttgccacgcc ggtcagggtg ttagcgcctg
     3841 ccggtttcag ggtcgcgctg ctgctgtcga aggtcacatt gttcggcata ttgaggataa
     3901 tgttatcccc gctgcgggtt acgctaacac cagtgccgcg cattttgtca cgcagcttcg
     3961 cttcctgcac atccatgtaa taacccacgc cgccgcccag agctgcgcct gctgctgcgc
     4021 caatcagcgc gcctttaccg cgatctttct tcgaagaaga gagcgcacca atacccgcgc
     4081 ccacgagaga gcccagacct gcgccgatag cagatttgcc tgcttcgcgt tcgccggtgt
     4141 aagggttagt tgtgcagcca gataccgcca gagcgccact cactacggcg gcaataagat
     4201 aaacacgttt cttcattgtt aatccttaat aaccttttta ttctttgcca cgggttccgt
     4261 ggcgggagat tatgccgcgt gaacatgaa
//
LOCUS       DAZOZY010000270         4261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZOZY010000270 DAZOZY010000000
VERSION     DAZOZY010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.273"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..3511)
                     /locus_tag="ACHURQ_003994"
     CDS             complement(<1..3511)
                     /locus_tag="ACHURQ_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR0825638.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4261)
                     /locus_tag="ACHURQ_003995"
     CDS             complement(3963..>4261)
                     /locus_tag="ACHURQ_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0825639.1"
                     /translation="VNKALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQM
                     SHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1098 a         1095 c          878 g         1190 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatttaca
     4261 g
//
LOCUS       DAZOZY010000271         4181 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZOZY010000271 DAZOZY010000000
VERSION     DAZOZY010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.274"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHURQ_003996"
     CDS             <1..1359
                     /gene="dcuC"
                     /locus_tag="ACHURQ_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR0825640.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHURQ_003997"
                     /gene_synonym="citB"
     CDS             complement(1400..2080)
                     /gene="dpiA"
                     /locus_tag="ACHURQ_003997"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR0825641.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHURQ_003998"
     CDS             complement(2049..3707)
                     /gene="dpiB"
                     /locus_tag="ACHURQ_003998"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR0825642.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4087..>4181
                     /locus_tag="ACHURQ_003999"
     CDS             4087..>4181
                     /locus_tag="ACHURQ_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825643.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSV"
BASE COUNT         1030 a         1062 c          966 g         1123 t
ORIGIN      
        1 ggggttgtgg ttattgtggg tgtagctcgc tacatcatta aagggtattc cgccactggt
       61 gtgttatttg tcggtggcct gttattgctg attatcagtg ccattatggg gcacaaagtg
      121 ttaccgtcca gccaggcttc aacaggctac agcgccacgg atatcgttga atacgttaaa
      181 atattactaa tgagccgcgg cggcgacctc ggcatgatga ttatgatgct gtgtggattt
      241 gccgcttaca tgacccatat cggcgcgaat gatatggtgg tcaagctggc gtcaaaacca
      301 ttgcagtata ttaactcccc ttacctgctg atgattgccg cctattttgt cgcctgtctg
      361 atgtctctgg ccgtctcttc cgcaaccggt ctgggtgttt tgctgatggc aaccctattt
      421 ccggtgatgg taaacgttgg tatcagtcgt ggcgcagctg ctgccatttg tgcctccccg
      481 gcggcgatta ttctcgcacc gacttcaggg gatgtggtgc tggcggcgca agcttccgaa
      541 gtgtcgctga ttgacttcgc cttcaaaacg acgctgccta tctcaattgc tgcaattatc
      601 ggcatggcga tcgcccactt cttctggcaa cgttatctgg ataaaaaaga gcacatctct
      661 catgaaatgt tagatgtcag tgaaatcacc accactgctc ctgcgtttta tgccattttg
      721 ccgttcacgc cgatcatcgg tgtactgatt tttgacggta aatggggtcc gcaattacac
      781 atcatcacta ttctggtgat ttgtatgctg attgcctcca ttctggagtt cctccgcagc
      841 tttaataccc agaaagtttt ctctggtctg gaagtggctt atcgcgggat ggcagatgcg
      901 tttgctaacg tggtgatgct gctggttgcc gctggggtat tcgctcaggg gcttagcacc
      961 atcggcttta ttcaaagtct gatttctatc gctacctcgt ttggttcggc gagtatcatc
     1021 ctgatgctgg tattggtgat tctgacaatg ctggcggcag tcacgaccgg ttcaggcaat
     1081 gcgccgtttt atgcgtttgt tgagatgatc ccgaaactgg cgcactcttc cggcattaac
     1141 ccggcgtatt tgactatccc gatgctgcag gcgtcaaacc tgggtcgtac cctatcaccc
     1201 gtttctggcg tagtcgttgc ggttgccggg atggcgaaaa tctcaccatt tgaagtcgta
     1261 aaacgcacct cggtgccggt gcttgttggt ttggtgattg ttatcgttgc tacagagctg
     1321 atggtgccag gaacggcagc agcggtcaca ggcaagtaaa tagtaatgcc ggaggcaagt
     1381 ttcctccggc tttatcatgt cacccactgt ggtatatgcg ttgtggtctg ccaactttgc
     1441 cgtgaacaat ttcagcaata atcagatggc ggctggcgca atattcaaga taacgcctgg
     1501 cagtggtgca gctgatggtc agtgcctgcg ccaccgtttc tgccgtatgt tgcacaccag
     1561 gctctttaaa cagttttcgc accgcgttta gcgtcaaggg gtcaatgccg gtcggtagct
     1621 cgtccttagg ttcaccgcga gcataagcat taaacatctc atcaatttgc ttctggctgg
     1681 cgctatcaat actttccagc atatgtttac gctggcggaa acgggttagc gtttgcccca
     1741 gccgttcata ggcaatgggc ttaatgagat aatcaaatac accacaacgt acggcttcag
     1801 acaccgtttc catatcgctg gctgcagtgg taaacaccac gtcgccggga taatgcgcct
     1861 gcaccagttc atgcagtaaa ttaatccctc taccgtcagg aagatagtta tcgagcaaga
     1921 ttagccccgg cttaaaacgc tcgatcatca ttcgggcctg cgccagattt cccgccagta
     1981 atatctgact gaatccggga atgtgacgaa tatattccgc atgcatctct gccagcggcg
     2041 tttcgtcctc aacgatcaat agggttaatg gagctgtcat taggtttcac tttcggaata
     2101 tagattgaaa ataaggtacc gcagggatca ttatcttcga gagtgataac accaccgcag
     2161 cgcgttacgt agctggcaat caagtacaac ccaatgccat gttcaccggg ctcgtcagca
     2221 cgcgtactga ccccctgctc aaatattttg tctcttagag actctggaac gccgcagccc
     2281 tgatcggcga cttcaatcac cacatcatcg ccttcatcgc tgaggaataa ttcaacgatc
     2341 ttgtttcctt catcgctacg caggctggct tcgaaggcgt tatcaagtaa attgccaaca
     2401 atggcggcaa actcggtgct atccagtcct ggcggcagtt gcgaaagctg gctaccgggg
     2461 acgatgatca tttttagccc cagttcgcgg gcgcgctgca ctttaccaaa aagcagcccc
     2521 gccacctggc gatcggcaaa cgcctcacgc aggctgtcaa taagctgttg ctgggcctga
     2581 gactccccct gcaccatggc cagcacgcga tcatactctt tcatctgcaa cagaccattg
     2641 agcgtcgaca tccaattgag atgctcgtgt cgcaatgtac gaaggctctc gacgtattgt
     2701 tttatttgcg tcagttgcgc attgagggtg gaaatttcgt ctttactacg aaagctgata
     2761 atggccccca gcaaatcatc acctgaacga atagcttccc ggttggcaat aacgttcaga
     2821 ccattaaagt tcgccaccac atcctgacgt ttttcatcaa tctgttcggt aaagaaatcg
     2881 gcgggcctga ccacttcagc aatgggttta cccaaccatt gccgtccggg agagctcagc
     2941 cccagcatct ttcttgcgtt acgattgatg gcggtaatgt aaccatgcgg atccaccgca
     3001 atcagccctt cataaaccga actaaacagc gcctcttgct gacggaccac gcgtgcgatt
     3061 tgctttggct ccatgcccat catttgtcga cggatatgcg cggccaggaa ccacgacagc
     3121 aacatcagaa tccctaacag cacgacaaac acgcctgcca tcggtaataa aaactcagcc
     3181 cgccagctat cgattttact caccaggtag ccaatcgaca ccacgccgat gacttttcca
     3241 tcgtcatcaa agattggcgt tttggcgcgc atcgccatcc ccattgcccc tttcccggta
     3301 atgaagtagc tctccccttt ctccagcgcg ccctgtttgg tgaactgcat aggataacca
     3361 attttctccg gattaagatg gtaaaggcgg atcgagtgcc ggtcgccaat caccacataa
     3421 tcaaaatcgg tatctctttg taatttgtta gcgatggtcg ccagccgttt gtagtcacgc
     3481 gttttcaccg cagagatgac actgtcattg gaggcaataa ttttcgcctg attcattgcc
     3541 atgtcgcgta catgaagcgt taaatagtcc tcaaaactgg ccgtaaaata ttgcgccagg
     3601 gctgcaatga caaatattga gaacaccaga atcagcaaaa agatacgcag cggaaatgcc
     3661 agtctttgga aaaatgcaaa ctgtttattc tcgttaagct gcaacattgt tttccttgcg
     3721 ggttgtcccg agcctctttg acggatgtaa ttaaaatgtt gaatcaaaat acatagatat
     3781 taattatgat cgtggcaaat tttattcatt aaatcaatta aatcacttaa acctgcaggt
     3841 catttcgtaa ttacattaat taaaaaactt aaaaccatta aataaataaa aaaccactaa
     3901 ctctatgtga aataaatcaa aatttcacgc tgcaatactc cttagaatgt atagcgaaag
     3961 gagaaaaaga tatacctcga tcacccccct ttctcccaag tgaaaataaa aggttatcaa
     4021 cttgcaacat tgaacaacat tcgttgcaaa tcgataacaa catgcacctt caggatacta
     4081 tttattatgt tcggcaatga tattttcacc cgcgtaaaac gttcagaaaa taaaaaaatg
     4141 gcggaaatcg cccaattcct gcatgaaaat gatttgagcg t
//
LOCUS       DAZOZY010000272         4127 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZOZY010000272 DAZOZY010000000
VERSION     DAZOZY010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.275"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHURQ_004000"
     CDS             <1..122
                     /locus_tag="ACHURQ_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0825644.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="ACHURQ_004001"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="ACHURQ_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR0825645.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..3180
                     /gene="safA"
                     /locus_tag="ACHURQ_004002"
     CDS             2983..3180
                     /gene="safA"
                     /locus_tag="ACHURQ_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR0825646.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3255..4016
                     /gene="ydeO"
                     /locus_tag="ACHURQ_004003"
     CDS             3255..4016
                     /gene="ydeO"
                     /locus_tag="ACHURQ_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR0825647.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
BASE COUNT         1174 a          891 c          935 g         1127 t
ORIGIN      
        1 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgca gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcagggcgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1021 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccac agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgta
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatt
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgtatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct gggtatttca
     3121 gaaatgggtc gcattgcaag agatcttaca catttcatta tcaatagttt gcaagactga
     3181 aaataaacag caaattataa atatgaaatg ttaaaaaagt atcgataaaa actttattgt
     3241 tttaaggaga taaaatgtcg ctcgtttgtt ctgttatatt tattcatcat gccttcaacg
     3301 ctaacatttt agataaagat tacgccttct ctgacggcga gatcctgatg gtagataacg
     3361 ctgttcgtac gcattttgaa ccttatgagc ggcattttaa agagatcgga tttactgaaa
     3421 ataccattaa aaaatatcta caatgcacta acatccagac agtgacggtg cctgttcctg
     3481 cgaagttttt acgtgcttca aatgtaccga ctggattgct taatgaaatg attgcttatc
     3541 ttaactcgga agaacgcaat catcataatt tttcagaact tttgcttttt tcttgcctgt
     3601 ctatttttgc cgcatgcaaa ggtttcatta cactattaac taacggtgtg ctatccgttt
     3661 ccggaaaagt gagaaatatt gtcaacatga agctggcgca cccatggaag ctgaaagata
     3721 tttgtgactg cctgtacatc agtgaaagcc tgttgaagaa aaaactaaag caagagcaaa
     3781 cgacattctc acagattcta ttagatgcca gaatgcagca cgcaaaaaat ttgatacgcg
     3841 tagaaggttc agtcaataaa attgccgaac aatgtggtta tgccagtaca tcttatttta
     3901 tttatgcgtt ccgcaaacat ttcggcaaca gtccgaagag agtttctaag gagtaccgtt
     3961 gtcaaagtca catgggtatg aatacgggca acacgatgag tgctttagct atttgattat
     4021 ttgctaacga gtagtcaacc acgcacgctg cgtaagaatt aaatggggca gccattccct
     4081 gccccgcgtt gtttttaggc gatatattta ttgaaataaa taagtga
//
LOCUS       DAZOZY010000273         4075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZOZY010000273 DAZOZY010000000
VERSION     DAZOZY010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.276"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHURQ_004004"
     CDS             <1..784
                     /locus_tag="ACHURQ_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0825648.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1756
                     /locus_tag="ACHURQ_004005"
     CDS             797..1756
                     /locus_tag="ACHURQ_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR0825649.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHURQ_004006"
     CDS             complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHURQ_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR0825650.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHURQ_004007"
     CDS             complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHURQ_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR0825651.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1224 a          952 c          861 g         1038 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1141 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1201 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1261 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1321 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1381 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1441 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     1501 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     1561 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     1621 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     1681 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     1741 ggttatgtac aactaatcta tctcccccac cttaactttg ggggaggtgc cgattaacgc
     1801 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     1861 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     1921 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     1981 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2041 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2101 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacga tttttctggt
     2161 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2221 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2281 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2341 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccacg
     2401 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     2461 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     2521 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     2581 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     2641 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     2701 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     2761 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     2821 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     2881 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     2941 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3001 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3061 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     3121 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caatacatgc tccagtcgct
     3181 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3241 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaacaagaa
     3301 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3361 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3421 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     3481 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     3541 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     3601 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     3661 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     3721 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     3781 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     3841 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     3901 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     3961 actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4021 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacgg
//
LOCUS       DAZOZY010000274         4061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZOZY010000274 DAZOZY010000000
VERSION     DAZOZY010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.277"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHURQ_004008"
     CDS             <1..784
                     /locus_tag="ACHURQ_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR0825652.1"
                     /translation="FSVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSL
                     HREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQ
                     LQTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVI
                     NINSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSS
                     EDHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            781..1686
                     /locus_tag="ACHURQ_004009"
     CDS             781..1686
                     /locus_tag="ACHURQ_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR0825653.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1683..2753
                     /locus_tag="ACHURQ_004010"
     CDS             1683..2753
                     /locus_tag="ACHURQ_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR0825654.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2990..3343
                     /locus_tag="ACHURQ_004011"
     CDS             2990..3343
                     /locus_tag="ACHURQ_004011"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825655.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
     gene            3469..4008
                     /locus_tag="ACHURQ_004012"
     CDS             3469..4008
                     /locus_tag="ACHURQ_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR0825656.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT         1077 a          900 c         1101 g          983 t
ORIGIN      
        1 gtttagtgtc agtgccccgg gccatcattc atggaaacga gactttgatc cggacagccc
       61 cgagataaaa ttcagtgatc gcagggaagc ccttaaactg atgacgcaaa tcgaatcgac
      121 cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg cctgaactgg aaaaaatcgt
      181 caggggtatc gaaacaggtt ttcgtggtac ggccctgtat gccacagaaa atattgccgg
      241 acgtatcaat gcccgcctgg aggacgaggg cttcactgta aaaatcactt tcccggcagt
      301 cagccagtta cagaccagga tcgcggtaaa aacaaatctg agtgcgctta tggaggaaag
      361 aactgagaca gtcacccgtc gccgtcggca gagtggtttc tggggaaaga tttgtggagc
      421 gtttggcacc agtgactggg gctgggaaaa ctataaggag aatgtgagcc gcagtgtgat
      481 caatatcaac tcggtcagga aggaagtgat gtcactgacc cgggcatatt tcggggagtt
      541 gcaggcatcc attgagcaga atattaacca gcccgtccgc caggagatcg atgatttttt
      601 ctgtacattc agggaaaaag tcgaacagct acgtaacaca ctcattcaga gctctgaaga
      661 tcataaacgc gatcagcagg cacaggagca gcttacggag cgccttcagg cattaaatga
      721 aagggttccc gagctgatta ctgacagtaa ggcgttaagg gaagaactgg agacactgct
      781 gtgacctcac catttattca acaaattgct gataaccggg tatgtcaggt actcagttgc
      841 cttcctgaaa aatttgtggt tgattttgca aacggtattg atgttgcaca ggagcataac
      901 cgcacggccg gggggcgcac gtttttccgg cgtttaaaag aaggtctgac tggcaaaggc
      961 gctgtacgcc agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc
     1021 tggctgacgg agctgacaac gtcgcttgcc accacaaatt acgcgattac ccgggtaaac
     1081 gacagggtca gttcactggt cagtgatact gccaggctgg cgcattattc ggcagacacg
     1141 cgggagcagt tactcaccct ggcagaacag gttcatcaga aactgaatca tcttgaagaa
     1201 aaactccacc gtgttgacca ggttcagcgg gcacagttac atcttgagca gatattctca
     1261 tggtggagcg ccgggcgata cgcgtcattt tcccctgccg gacgttgtta tgtggcgctt
     1321 gaagagcttc gctggggcgc gtttggtgat gtgatacgtc agggtgaaac aggccaggtt
     1381 aaccagttac tggatatcct cagacataaa gcattaacgc aaatggcaca ggagaatggc
     1441 ggtagcgcaa cagtacgtct gaacacactg gactggcttg gtggtcagag ccgggaacag
     1501 gctgataacg aatggcatga agcggttaac tggctggggg actggtgcag tgaagagcgg
     1561 catcctgtga tctggtcaac cacacaggct gcagaacact taccggttcg tatgccccgt
     1621 ctctgctctg cagaacgcct ctctgaaagt atggttgatg aaatatttca gaagggggag
     1681 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg cggcggggcg gtgggcgctt
     1741 atcaggcggg agtggttaag gcactggcag agtgcggcgc acagatcagt atggtctcag
     1801 gagccagtat cggggctctc aatggtgcca ttatcacggc ctctcccgat ctgtcagaag
     1861 ccgccttacg cctggaggcg ctctgggatc atctggggaa taatcaggtt ctgtcggtaa
     1921 acagatcggt ttacttttca ttgctgaaaa aactggttca ggccatgaac ctctgccaga
     1981 tccccggacg tgcgggcgca ctgcttacga cgctttttcg ccatatatcg acaatcaacg
     2041 ggttcgacaa tccgatgatt cagccgctgt tgtcagatga gcccctgaca gcgctgatgg
     2101 atcattatct tgatacagat gctctggccg acgggctacc gctgtacgtg tcgctgtacc
     2161 ccacagaagg aggcatgcag gatattattg actgcattcg tgctgaactg ggtgccggaa
     2221 ccacgaaaaa cgctgttttt cagcatatcc agagtctgcc ccgcgggcag cagaaagaag
     2281 ccttactggc gtcagccgcg ctgcctctgc tgttccgtcc ccgcgaggtt cagggaacaa
     2341 tgtacggtga tggtgggatg ggcggatggc gaaacaggca ggggaatact cccgtgacgc
     2401 cactggtgga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     2461 gggatcgtcg ggcttatccg gacaccacaa tccttgaaat ccgtccccgg aaaaggttga
     2521 aacaaatcgg ggacgaaggc aaaagtggcg gtctgctcag ttttacatcg gcacataccg
     2581 acgcctggcg tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac
     2641 cgctggcagc acgtcaggca ctgacccggt cagagacggt attgcagaaa agcctggaga
     2701 taactgaagg tgcagattcg gcactgagaa acgcgatggc ccggattaaa taaagacgct
     2761 ccggagaaac gccaccgcca ctgtggtggt gtttttcctg tctggtatta ttgaaaatat
     2821 tttcagatat atattacctg gatgatggtt aagattaatt tatttaattt tggggagaaa
     2881 actcatgaaa ctgttaaaca ttcttttagc tgttgtttta actgtaagtg caaccgtagc
     2941 acaaacagaa tcggcattgt agacgcgtaa cagtattgct ggcactgata tgttcagtat
     3001 cggtaatgtt tctattaact gcacgattaa tccagaagac aacaatttgc taccacacta
     3061 tgtgtggatt tacggtcata cctccaccca gctaaatcag tacgattctg atattatttt
     3121 tatcatcaac gataaaaagt atccggcccc atctgtcgac ggaacacgaa tgaataagaa
     3181 tgcatgggta tactttatcg atgctatcag tgaagccacg caattcgacg ttttagtaaa
     3241 cggtaagaaa gtcgacagct ataccgcaaa tatcaaaaat gtgaaaaaga ctttgggtaa
     3301 caaattctac gacagctgct ggaacacctg gttccaggaa taattgataa cgtggtgtaa
     3361 aacacctcag tttaatttaa tacaatgagt gaaaatgaat attataaaaa tattttttgt
     3421 ttcagatgtc atatatttta ttaactgaat gattaacagg agaatcagat gaactggcat
     3481 tatgaaaaaa atggtgtacg ttatgataac gtaacagagg aagatattat cgaacgtatc
     3541 cggcgtggtg aactgaatgc atcaacactg gtgtggcaac agggcatgct ggaatggcag
     3601 ccagtttcag ccacatcact ggcggaaata ctgaaacaaa gtgaagttcc cccagccctt
     3661 ccgggtcacc gcattcccgg aggcgttgtc tggacgctgg catttgcccc tttgattggg
     3721 tacatacttg agatgtggac tgcagggctg aacggtatgt catttgaacg ggcttatgat
     3781 gcagtcagcg aaggccagta ctggtttatc tctctgattc tgaatattgc tctgggatat
     3841 ctggatgaac gaaaactccg taaatctggt gttgatactg caacatttgg ctggctggca
     3901 tggttgatcc cggtttatct gtggcgtcgg gcaaaaatcc tcggacaaaa acctgcgtat
     3961 ttctgggtct ggttggttat gctgatactg gtgttattta cggtctgata ttctttatcg
     4021 cagagtttac taaataatca ggttattagt gttcttaaca g
//
LOCUS       DAZOZY010000275         4045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZOZY010000275 DAZOZY010000000
VERSION     DAZOZY010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.278"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHURQ_004013"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHURQ_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR0825657.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHURQ_004014"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHURQ_004014"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR0825658.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHURQ_004015"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHURQ_004015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR0825659.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHURQ_004016"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHURQ_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR0825660.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          969 a         1141 c          971 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacc
//
LOCUS       DAZOZY010000276         4042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZOZY010000276 DAZOZY010000000
VERSION     DAZOZY010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.279"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            376..483
                     /locus_tag="ACHURQ_004017"
     CDS             376..483
                     /locus_tag="ACHURQ_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0825661.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHURQ_004018"
     CDS             complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHURQ_004018"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR0825662.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHURQ_004019"
     CDS             complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHURQ_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR0825663.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHURQ_004020"
     CDS             complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHURQ_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR0825664.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1034 a         1011 c         1051 g          946 t
ORIGIN      
        1 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
      181 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
      361 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
      421 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
      481 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      541 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
      601 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
      661 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
      721 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
      781 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
      841 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
      901 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
      961 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
     1021 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
     1081 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
     1141 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
     1201 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
     1261 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
     1321 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
     1381 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
     1441 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcagcttct
     1501 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
     1561 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
     1621 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
     1681 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
     1741 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
     1801 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
     1861 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
     1921 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
     1981 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
     2041 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
     2101 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
     2161 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
     2221 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
     2281 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
     2341 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
     2401 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
     2461 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
     2521 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
     2581 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
     2641 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
     2701 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
     2761 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
     2821 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
     2881 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
     2941 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
     3001 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
     3061 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
     3121 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
     3181 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
     3241 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
     3301 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
     3361 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
     3421 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
     3481 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
     3541 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
     3601 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
     3661 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
     3721 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
     3781 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
     3841 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
     3901 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
     3961 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
     4021 gattatcagt ttactagcga aa
//
LOCUS       DAZOZY010000277         4009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZOZY010000277 DAZOZY010000000
VERSION     DAZOZY010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.280"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..353)
                     /locus_tag="ACHURQ_004021"
     CDS             complement(<1..353)
                     /locus_tag="ACHURQ_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0825665.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRE"
     gene            complement(368..1114)
                     /locus_tag="ACHURQ_004022"
     CDS             complement(368..1114)
                     /locus_tag="ACHURQ_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR0825666.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            complement(1121..1429)
                     /locus_tag="ACHURQ_004023"
                     /pseudo
     CDS             complement(1121..1429)
                     /locus_tag="ACHURQ_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1808..2320)
                     /locus_tag="ACHURQ_004024"
                     /pseudo
     CDS             complement(1808..2320)
                     /locus_tag="ACHURQ_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(2333..2755)
                     /locus_tag="ACHURQ_004025"
     CDS             complement(2333..2755)
                     /locus_tag="ACHURQ_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR0825667.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2752..2994)
                     /locus_tag="ACHURQ_004026"
     CDS             complement(2752..2994)
                     /locus_tag="ACHURQ_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0825668.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            3091..3510
                     /locus_tag="ACHURQ_004027"
     CDS             3091..3510
                     /locus_tag="ACHURQ_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0825669.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3816..3971
                     /locus_tag="ACHURQ_004028"
     CDS             3816..3971
                     /locus_tag="ACHURQ_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR0825670.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT         1007 a         1075 c          891 g         1036 t
ORIGIN      
        1 cactcgcgac agattatatt caggcgcctg tcgtagcggc gtatttctcc gtctggtaat
       61 gaccagataa ggtcaggatc aaccacaacc ggtttcttca cctttaccct cgatagcttt
      121 ttgcgggcgt tttgccagtc tttatgagcc tgttcagagg gaaatacccc gtagccagaa
      181 ttatatacat caccacttgc gaccagttcc atgcataagc gaccgacaga cgcacgactg
      241 atacctgttt catctgacaa ctgccgaatc gtgacccgac cgtcctgatg cacgagttcc
      301 acaattcgcg tcttcagttc ttcccgctgt tcgggagtaa aaggtttcgc cataaatcct
      361 cctgaaacta cttaacaacc ctcgaatggc taacattcgg acgccagctc tcccagttaa
      421 aattcaccca gcgcccgccg ttcatgacca tgcgatccat cacccgctcg ccgagaagtg
      481 tattcatcgc tgcatggtta atatttgtca gcatccccac accgcgtaac gatgccgtcc
      541 ggcgatcaac aatctggttc agtaccacct gctcgttttt cgtatcccgc tgcatgccaa
      601 tttcatccag gaccagcagg tcaacaccac aaagctcctg taaaaatttt tcaccggatt
      661 tgccgttgtc gtagctctca tgcaacacgc tcatgacatc ggacacggtg acgataatca
      721 cgctacgccc cttcgccatc agccggttgc caatcgcgtc tgcaagatga ttttttccgg
      781 tgccgggctt accgctgaac acaaaattcg tacagcctgt catcagttcg tcagctatgg
      841 atttcgcctg actcagtgca tgtcgctgac cgtcgttctg cgcccggtaa ttcgcaaacg
      901 aacacttccg gtgcaacggc tggatgccgg agcggttcag gattttttcc acccgcaact
      961 gacgattcag gcggttgatc tcctcgcaac gtttctggcc ttcagcaagt tgccactcgc
     1021 gccactccgc aaccgttctg aatggggcgg ttacatgtgg tggggtcagt ctgcggatac
     1081 gctccagaac gcctcctgtc gcaatatttt tcatggtctg ttaccccctg aagcctggcg
     1141 ggatcgcact gtccggcaac gagacggtgt taacctgtcg gagcaacgtc tcaggccgaa
     1201 cacctttcgg cgcgaacaga ccctggtatt cattggcgat gctgtgtcga atcacctgct
     1261 caggtgtaaa accctgctga cggaattttt ccagttcccg tatcgccccg ttagcgccct
     1321 gctccgttcg aatcggtttt cgcaatgcct gcctgaactg gacccactca tgccagagtg
     1381 tttctggcaa ccagtcgggc agatcaactg acaacgggtc aaattttttc tggcgaacag
     1441 ccccaccgat aaccagaatt ccctgctcgg tgtgccttgc gatatcactg cgccgcaaga
     1501 cacatatccc ggtttcaaca gcaccgattt tgagcagtga tttctgtcgc ggagaatttc
     1561 ggtcaagctc gctcccacac cggacaccgt tcctgtcagt cacgacgata tcaatccgcc
     1621 ctccgcaacc gtcaccacgc tctggcaccg gatactcacg acagacatcc aggccagcag
     1681 cctgcaacgc aaccaccgca gaattacaaa attccgctgc agtaccgcca gataatctcc
     1741 cctcaagcac tccagccaca aaatcccgaa actcccttcc ggaagggaag ggtttgggat
     1801 gggttagatc tgtttttaga tctttatctg tatctttatt agttgccttt gtgttggcat
     1861 catgttcgaa catcactcca acatctgttt gaacacctgt taaatttctc tcttgttttg
     1921 tttgaacatc tgcttccttt ctgcttcttc tggcctgaac agatgctttt cctgcggctg
     1981 attttttggt taatttttct ctgacagatg ccagatcttc ctcaatccga agatgcatcc
     2041 attcgtcgcc gttatcacaa aaaaactccc gcaaggatgg ttcaacatca acccatcgct
     2101 cgttagtcag acgggcaatt tttgccaacc tgtttttggg tattggcttt cctgtttgcc
     2161 agtaattgaa catcagcaac aaatacgcgc cgtgctcctc tgctgacaaa tgcatggtgt
     2221 cagccaggta atcagctatg tacagttgca tgtatggtaa tgcggccata attgccccgt
     2281 atgatgctgc ccggttgctt agaataagca caaacagcat ggaaactttt gcttaatgaa
     2341 caatgacaga atcgtcggaa gacccgccgc cgctgaaatg cgctttccgg taaacggcct
     2401 ggactgcatc atcatgcgca tcaattgccg tactcaacgc ttcctgcgcc gccagtaatg
     2461 cacggcgttc cagggtatcg aagatgcaga gtcggtgacg cagctcgcgc ggaagaattg
     2521 ccagaaccgc agggatcagt ttctgaattt tttccctttg cgctttcgtt tcacctttca
     2581 accaacggtg atagatattc tgctgattgt tccagtcctt gcctggtaca aggggcaatt
     2641 cgccgccccc ctggcgcaga tattcttcag taattgcgtt agcgacccac gcctgccctt
     2701 tttcggctgc cagggctaac aacactgatt cgatgtgctc atgcctgatt ttcatgaatc
     2761 aaccgctcct atgctgtttt cgctatgctt accgtctggg gggaatacat cgtcaagtcc
     2821 acaatgagcg ccaagccgat taagggtaga aacaattttt ctgcactcct ctagtccagg
     2881 ggtacgaaaa tttgcttcgt aatttgccag tcggctttgt atccacccta actgaacagc
     2941 aagttgtctt tgagacagcc caagctgttt tcgatatgtt gaaattttgt tcattgaaaa
     3001 cctccgatga caattttaaa cacaccttgt gttatatagc caagctgttt tgtgttttat
     3061 gtaaatcacg attcgtgata caaggatgca atggaaaaag aaaacgaaaa aattgccgct
     3121 agtaggctca atgacaaaat tgcaatgcgt cttaaagagc gcaggcagaa gcttggttta
     3181 tctcaaggaa aacttgctga aatctgcgga tggacgcaat cgcgtatagg taactatgag
     3241 gcgggcagca gaaatgttgg agtacatgac gctgtcgtat tgggaaaggc acttggcata
     3301 tctcctcctg agctcctctt tggagaacag gaatcttctg aattgtggtt aaatgaatcc
     3361 caacgaaaac ttcttgagtt gtttaaccag ctaccgggct cagaacaaca acgaatgatt
     3421 gagctatttg aagtccggct aaaagaaatc gatgagtatg tagaaaaata tttgagaggc
     3481 aggattaaag ataatccccc accggaataa tgatcttgct atcacagtaa tatgccaatc
     3541 agcccgctat cagcgggctt ttttgtagca tcatcatatg acactcacca taaaacacat
     3601 tgcgtgttga cacaagaaaa caaattgtgt ttaataagca tatccaaaca acgccccacc
     3661 agagaacggc aggacaatac ctcgagttat ccagtcactg aacagggcta agtagccagc
     3721 ctgaggcata cgaacatgac ggcagttgtt gattgataca aagcgcagta gataaaacgt
     3781 tccgccaccc ggcgttaagg ggaaatgggg tcaacatgaa tactatcgat cttggcaaca
     3841 acgaatctct ggtatgtggc gtgtttccca atcaagacgg cacattcacc gccatgacgt
     3901 ataccaaaag caaaacgttt aaaaccgaag ctggcgcacg tcgttggttg ggaagacatt
     3961 caggtgagta aaatgaacga gacagaatta aaacacgtta tcgctctac
//
LOCUS       DAZOZY010000278         3995 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZOZY010000278 DAZOZY010000000
VERSION     DAZOZY010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.281"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            22..1413
                     /locus_tag="ACHURQ_004029"
     CDS             22..1413
                     /locus_tag="ACHURQ_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR0825671.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            1410..2486
                     /locus_tag="ACHURQ_004030"
     CDS             1410..2486
                     /locus_tag="ACHURQ_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR0825672.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            2464..2997
                     /locus_tag="ACHURQ_004031"
     CDS             2464..2997
                     /locus_tag="ACHURQ_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR0825673.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            3003..3413
                     /locus_tag="ACHURQ_004032"
     CDS             3003..3413
                     /locus_tag="ACHURQ_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR0825674.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            3406..>3995
                     /locus_tag="ACHURQ_004033"
     CDS             3406..>3995
                     /locus_tag="ACHURQ_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0825675.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
BASE COUNT          893 a          978 c         1189 g          935 t
ORIGIN      
        1 tttttttata gtggagacag tatggactta ccttctttac cttctctttc ttcactcttt
       61 tctgcatctt caggtatcaa ttggcgcgat aatctttacg acgcttcatt tcgtggcgtt
      121 ccgttttcgg tggagagcga cgagggttcg ttcggacgcc gcgttcaggt ccatgaatac
      181 ccaaaccgtg acaaaccgta cacggaagat ctcgggcgtg ccacgcgacg gctgacgatt
      241 aatgcgtatc tcgttggtga tgattacgca gagcagcgcg acaggctgat tacagcgatt
      301 gaaaccgccg ggccggggac gctgatccat ccgcagttcg gtgaaatgca gggctgtatt
      361 gacgggcagg tgaccgtttc tcactccggc actgaaggcc gtatgtgccg ggtttcattt
      421 cagtttgttg agagcgggga actgtcattt ccggtcgccg gagctgcaac cgccagaaaa
      481 ctggaggagt cgtccgtatt cctggatgag ttgattgaag acatgttcgg cgattttgat
      541 ctcgcgggaa taccggactt tattcagaac gatgttattg cccggacgac cgatatgctg
      601 ggaaccgttc agacagcttt caaaatggtt aattcggctg tttctgccgg agcgagacta
      661 ctgcagggcg atttatccgt cattctgatg ccgccgtcgg ttgccagtga ttttgtgcat
      721 acgttgcagg atacctggcg ggccggaacc cggctggtgg ataacacaca ggatctggtg
      781 cagtccataa cgacaatgtc cggcattacg ctggacccag gactggcacc gcgtgcggtg
      841 tggcccacag attccgcatc ggttatcagg caaaaacagc agacaaacct tgtggctgct
      901 gtcatccgga cgacggcaat cagcgaagcc acaagggcgg tcacttcact gccacaaccc
      961 ggaagcctgg tgaaaaatca gcaggcggtt gtggctgttg gtggttccac tgaacgtcag
     1021 tccgatatta ttcacgtttc tcatccggca cttgacagcg tggcagccag cacaggacag
     1081 gatgagagag cgcaaccacc cacgcgggaa aatctcacca tcatacgcga atcgctgaac
     1141 gcggcgattg aacaggagct caggcgtacg gcggatgaca ggctgttttt tcagctgacg
     1201 tcattacgta cagaactgaa ccgggatatt caggcgcgtc tggttcagac ggaggaaacc
     1261 gcagagcgaa cgccagcgga agttctgcct gcgcttgttc tggctgcgtc atggtacgac
     1321 gatgcgtccc gtgaaactga tatcctggat cgaaatgcca tctctcatcc gggctttgtt
     1381 ccggtcaggg cattaagggt acccgtcaga tgaatgatac cgttttactt cgggtttccg
     1441 gtcgcgaatg gggcggctgg acatccgtcc gtatcagtgc gggcattaac cgtattgccc
     1501 gggattttaa tgttgccatt accacccgct ggcccggaag ccgggattat caaccccgga
     1561 taaaaaatgg tgagctggtt gaagtgctta tcggggatga gcctgtgctc accggatatg
     1621 tggaggcact accgcttcgt tatgacgcca gcagcgtcag catgggaatt gtcgggcgaa
     1681 gcaaaacagc cgatctggtt gactgctctg ctttgccact ccagcagagc ggaaaaaacc
     1741 tgctcagaat agtcagtgaa ctggctgcgc catttggcat caccgttgtt gatgctggcg
     1801 tgccgcagac agcggtgatt gatgcccagc cggaacacgg cgaaaccgtt gccgattgtc
     1861 ttaaccggtt actggggcag gttcagacgc tggcttatga cgatgaatgc gggcgactgg
     1921 ttctgggaaa acccggaaca ggtaaagcgg cgacggcgct ggtgctggga gagaatattc
     1981 tttcctgtga cacggaaaga agcatcagag agcggttttc tgaatatcag gtcagtgggc
     2041 agcgcccggg caacgacgat gattttggtg aggccaccat tgccgcaata cgtcagacca
     2101 ttcaggacag tggcgtgacc cgttatcgcc ctttgttgat tcagcagtca ggcacagcaa
     2161 cgacagcaac ctgtaaggcc cgttgtgaat ttgaagcgcg ccaacgggct gcgcttaccc
     2221 gtgagacaac atatacggtt cagggctggc ggcagggcag tggcgcgtta tggcgtccgg
     2281 ggttatctgt catcgttttc gacccgctga ataattttga taatgatgaa ctggtgatcg
     2341 cagaagttac ctataaccag gacgaccggg gcacgacgac tgaattacgg gttggcccgg
     2401 cagatgctta tctccccgag cctgttaccg ccaggaagaa aaaaatgttg aggaggattt
     2461 ctgatgaacg ggttttctct tcgtaatctg atttcccggg ctgtcatcac ggcggtggat
     2521 tccgccagaa agtgtcagtc tgtagggttg aaaatgatag ccggagatca gaaacaacac
     2581 gttgagcacc ttgaacctta tggttttaca tctgccgcac agaacggtgc tgagggcgtt
     2641 gctttatttc cggcgggcga tcgttctcat ggtgtggttg tggtcgtggc tgacagacgt
     2701 taccggctga aaggactgaa acgtggggaa gtggcgcttt atgacgatct gggacagtcc
     2761 gttgtcctga cccgttccgg tattgtggtg gacggggccg ggaaggccat aatttttaaa
     2821 aacgcgccta aagcgcgctt tgaaatgcca gtcgaatcca ccgccgatat tactgacaat
     2881 tgcgacagtg gcggactcag catgcagcaa atgcggcagg cctacaatgc ccacaaacat
     2941 accgaaaatg gtgatggtgg cgggatcact gacacgccgg atcaaccgat gggctgaaaa
     3001 tcatgatgat taatgttaac gggcgacccg tgtcgaccgg ggcttcgatc gaccttctga
     3061 cgcgtgctgt gattatttcg ctttttacat ggcggcgtgc cgggcgtgat gatgcaccac
     3121 ggatatttgg atggtggggg gatacctggc ctgcggttca gaatgatcgc acggggtcgc
     3181 gtttgtatct gttgcgacgc agcaagctga caaataaaac cccgcagctt gccagagatt
     3241 atgtccgtga ggcgctggcg tggatggtgg aggatggtgt tgcttcccgt cttgatatta
     3301 acgctgtccg gaccgggaca gactcgctgg cacttgccat taccatttac cagcgtgacg
     3361 gcaatattca caacattatt tttgatgata tctggagtga actgaatggc tgacagtcag
     3421 ttttatcgcc ccggcctccc gcaacttatt tctatgatcc ggagcgattt attaacccgc
     3481 tttgagcagg atacgctgct tcgtcgtatg gatgcggaag tgtatgcccg tgtacaggct
     3541 gcagccgtac acacgttgta cgggtatatc gattatcttg ccagaaatct gttaccggac
     3601 gtgtgtgatg aagactggct gtaccggcac gccagaatca aacgctgccc gcgaaaagaa
     3661 gcggtggcag cccggggatt tgtgcgctgg gatggcgtag aggggacgcc ggtattgcca
     3721 gcgggaacgc agatccagcg tgataatcag gtgaccttta ccacgacggc gacggtgacc
     3781 gcagccgatg gtcttctccg ggtgcctgtt gtggcagacg aaccgggatc ggcggggaat
     3841 acggatgatg gtattgccat gcaactggga acacccgtca gtggtctgcc gtccacaggg
     3901 tacgctgaca ccattacaga cggtgaagat attgaaaatc tggaaatatg gcgtgcccgc
     3961 gttatggaac gttattacta cattccacag ggggg
//
LOCUS       DAZOZY010000279         3961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZOZY010000279 DAZOZY010000000
VERSION     DAZOZY010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.282"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHURQ_004034"
     CDS             complement(<1..242)
                     /locus_tag="ACHURQ_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0825676.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHURQ_004035"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHURQ_004035"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR0825677.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHURQ_004036"
     CDS             2255..3904
                     /locus_tag="ACHURQ_004036"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR0825678.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1032 a         1001 c          928 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 g
//
LOCUS       DAZOZY010000280         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZOZY010000280 DAZOZY010000000
VERSION     DAZOZY010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.283"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            30..968
                     /locus_tag="ACHURQ_004037"
     CDS             30..968
                     /locus_tag="ACHURQ_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR0825679.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="ACHURQ_004038"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="ACHURQ_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="ACHURQ_004039"
     CDS             2310..2603
                     /locus_tag="ACHURQ_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR0825680.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2768..3946
                     /gene="fadK"
                     /locus_tag="ACHURQ_004040"
                     /pseudo
     CDS             2768..3946
                     /gene="fadK"
                     /locus_tag="ACHURQ_004040"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1003 c         1088 g          926 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgcgg tggtgaatct cgatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaat
//
LOCUS       DAZOZY010000281         3939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZOZY010000281 DAZOZY010000000
VERSION     DAZOZY010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.284"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..588
                     /gene="fimZ"
                     /locus_tag="ACHURQ_004041"
     CDS             <1..588
                     /gene="fimZ"
                     /locus_tag="ACHURQ_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR0825681.1"
                     /translation="RMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDL
                     PGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPV
                     QMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLL
                     LSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(591..1106)
                     /gene="sfmF"
                     /locus_tag="ACHURQ_004042"
     CDS             complement(591..1106)
                     /gene="sfmF"
                     /locus_tag="ACHURQ_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR0825682.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(1117..2124)
                     /gene="fimH"
                     /locus_tag="ACHURQ_004043"
     CDS             complement(1117..2124)
                     /gene="fimH"
                     /locus_tag="ACHURQ_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR0825683.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(2137..3911)
                     /gene="fimD"
                     /locus_tag="ACHURQ_004044"
                     /pseudo
     CDS             complement(2137..3911)
                     /gene="fimD"
                     /locus_tag="ACHURQ_004044"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
BASE COUNT         1097 a          971 c          861 g         1010 t
ORIGIN      
        1 agaatgtcta ttgaagttct gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg
       61 gatgattacc gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg
      121 gatatagact tgcccggaac agacggtttt accttcctga aaaggatcaa acaaatccag
      181 agcacagtga aagtgttatt tttatcatcg aaatcagaat gcttttatgc tggcagagcg
      241 atacaagctg gtgctaacgg ttttgtcagt aaatgcaatg atcagaatga tatttttcat
      301 cccgttcaga tgatcctctc cggatacacg ttttttccta gcgaaacgct caactatata
      361 aaaagcaata aatgtagtac gaatagttca acggtcactg tgctatctaa tcgtgaagtg
      421 accatattac gttatctggt tagcggatta tctaataaag aaattgccga taagttatta
      481 cttagcaata aaacagttag tgcgcataaa tctaatattt atggcaaact aggtttgcat
      541 tcaattgtag agcttatcga ctacgccaaa ttatacgaat taatataata ttaattataa
      601 ttgatcataa atatcgcatc cgctttctcc acacctggct gaacaccgct ggctaacgct
      661 cgataattgg caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcggg
      721 ctcgcctccc ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgacggtt
      781 tgtgccattg cgggatcatc cagagccagc aggttggtat cggatgccgg cgttcccgta
      841 aataaaatcg caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt
      901 gccgtggtat cgccagcgtt cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc
      961 ttatcaatat ccgcagtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg
     1021 atattaatcg ttcccaaagg gtcagctgcc catccactgg aactccacag taacccgcag
     1081 aaacagctaa agagtactct tctcattctc cgtacctcat tgataatcga cgcgtaaata
     1141 ccccagcgcg ctaaacggcc cttcggtcgg tttttgaccg gtaatactga taggccaggc
     1201 gcgaagtgtg acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag
     1261 atcgttaggc gtgatcggcg tatcgttctg atcggcgaca ataaaaccta aatcctgatt
     1321 gtccgacacc atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc
     1381 ctgcgcagca acattcgtac atttgatcgc aatgctcttg gtttgcggca tgacgccagc
     1441 tggtcgattc cccggccctg ccgcactaaa taacgatgcg ccgatatcac caaaatcaaa
     1501 ttcaacaatc tgcccggcat ttaattcgca gttttgcggc acttcaaccc ggccaccaaa
     1561 actaatggta taaacaggtg taaccaacgg atcgtaaggc gttgatgtca catacacggt
     1621 aaacatcacc tgtctgggga tctccaccat gttaataaag ggacgaataa cctttaaacg
     1681 aaatatcaaa cgagaatcca tcaccccgaa cggaaggttt tgcgaaacgt taggatctgt
     1741 tcccatccga atataatttt gcggggggaa ctgaatatcc atcacactat caaccagact
     1801 catcgcacct aatagataat catttaattg catatattta taattatcga tagtttcctg
     1861 aacaataaag ttagtgacat aactacggta tgtataattc accagcgtgc cgggtggaca
     1921 aattgctgag acacctaccc agccggattt ttccggtaaa acaacaatct gccctggctg
     1981 gttattggtg ctattaaaga catttgagag atcatagtgt tcatcttctg ctacgccagt
     2041 actatttgca caaacagtag cccaggatat gtttgccaga cacaaacatg ccattgccaa
     2101 taataatctg cagattatct tcattgataa tttcctttat gagccaggat gtgtgcaaac
     2161 tgccgaaata acagtgacgg cttgctgtaa gctttgcttt ggcaatacat agtgcgcaat
     2221 gcagcgtgag ttcgcgccat ctccccactg aaccagtaat tcaccagaca atggcgcacc
     2281 acttaaataa acttgcccgt catcacccac catactggtt attccggtac tgttttcccg
     2341 taccagtgat ccaaacggca ccggttttcc gccctgggta acggtaatga gcgcccgcac
     2401 gcctatgcgg gtatcaaaat tggcacgaac caacgcgcct tgcgtcggca caacgctgct
     2461 aatatttttt tcaacatcga tggaattccc catcgtattg gtatcaagcg cgatacggtt
     2521 ataccgataa accgtggcat acggcatcac cgcatagccg cgccagtcgg ttaaaatgcc
     2581 agtttgattt tcaatgcgta caccgcctgc gccaggcgct ttaatcaaaa cattggtatc
     2641 ccctaaaggc tggctcagtg ttatgccatt ttcatgcccg accacaccgc cagacagctg
     2701 ccagttaacg tcatgttgat cgcggtcgta gttatacccg acccccagcg taccgtaagt
     2761 ggcctgccag tttgccgtgg cgctgcccgt gtacccatta ttcgaggtat cgccctggct
     2821 tacgtgataa ctcaggttgt ggccttccag taaggtccca ccaacgcctg ccagccagct
     2881 attttgcccg ttgctgttac ggttagcgtt caaggaggca taagcgcggt cgagcgcatt
     2941 ttcccgggtg tagcgacgtt tggtcaaaac attgaaaggc actgaaacat taagtccgac
     3001 aatatgttcg ttatcgggga tccctacaga ttcattccac gaaaatgaga gtgaataact
     3061 gatgccaacc cagctgctgg tataccccac ctgataccag gtatctgaat ccgaagtatt
     3121 ccagtatttt tgatgggtac cagagatata aagcgagcca aagtcattaa gtgattgtga
     3181 aatatttaac tgcaaacggt ctttgcggct aaagcgtaaa ttgtggtaat tcacgattat
     3241 cggttcatcg cgatgctcac cgtcgtaatc gtaatcatat tcgtacccct ccattcgacg
     3301 atacgcaaca tcatcaaggg tataaaaacc ttgtgtcgaa tagcggtaac ccattaactg
     3361 aaaattggtg ccgaaggtgt acatcgattt cgcatagagg aagcgaatgg aatccccctc
     3421 atgacgactg tcgtcggcta actggctgcg cgcatgggtc acatccagcg acactgcgcc
     3481 ccagttcccg aggttgcgcc ccagccctaa taaaaaggcg gtgtaattgg cagataattg
     3541 cgtcccgccg taggcagtaa attcctgtgg taaaccaccg agtgccgtgc cctgaaaaaa
     3601 gaaaggtgat gattgctgac tattaccgct acgaaaatcg cccgccgtca ggtcaaattt
     3661 gaaacgccct tcgcgttgta aaatcggcac tgttgaatac ggaattgtgt aattctgcca
     3721 tcgcgctcgt cgatggtgac atccagatcg ccatttgaag atgtcgggtg caaatcagta
     3781 atttcaaaag caccggggga aacatagctt tgatagataa taaaaccatt ttgccgaatc
     3841 gttagctggg ccgccgtacg ggcaatccca cgtaccgttg gggcaaaccc ttgctggcta
     3901 tctggataca tattatcgga agaataaagc cgtacacca
//
LOCUS       DAZOZY010000282         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZOZY010000282 DAZOZY010000000
VERSION     DAZOZY010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.285"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHURQ_004045"
     CDS             24..590
                     /locus_tag="ACHURQ_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR0825684.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHURQ_004046"
     CDS             594..1169
                     /locus_tag="ACHURQ_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR0825685.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHURQ_004047"
     CDS             complement(1211..2263)
                     /locus_tag="ACHURQ_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR0825686.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHURQ_004048"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHURQ_004048"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR0825687.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHURQ_004049"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHURQ_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          952 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZOZY010000283         3827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZOZY010000283 DAZOZY010000000
VERSION     DAZOZY010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.286"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHURQ_004050"
     CDS             <1..196
                     /locus_tag="ACHURQ_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825688.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHURQ_004051"
     CDS             207..374
                     /locus_tag="ACHURQ_004051"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825689.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHURQ_004052"
     CDS             371..715
                     /locus_tag="ACHURQ_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825690.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHURQ_004053"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHURQ_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR0825691.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1671
                     /locus_tag="ACHURQ_004054"
                     /pseudo
     CDS             1383..1671
                     /locus_tag="ACHURQ_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1710..1988
                     /locus_tag="ACHURQ_004055"
     CDS             1710..1988
                     /locus_tag="ACHURQ_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825692.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1990..3048
                     /locus_tag="ACHURQ_004056"
     CDS             1990..3048
                     /locus_tag="ACHURQ_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR0825693.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3049..3429
                     /locus_tag="ACHURQ_004057"
     CDS             3049..3429
                     /locus_tag="ACHURQ_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0825694.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3426..>3827
                     /locus_tag="ACHURQ_004058"
     CDS             3426..>3827
                     /locus_tag="ACHURQ_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0825695.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT         1034 a          852 c         1046 g          895 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgagaaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc gtattaaatg tttacagtct caacaaagga
     1501 agagtggagc tgcgttaatt cattttgtgg atgggcttgt gacggatggt tttatttcgc
     1561 gtgcaaatga atttgtcaca tcgttgccgt ctctgaaaga agctgggatt aaggctggtt
     1621 tttctgcttt tgaagatgag tgaattcatc tacaattcag cgcagggctg aacccctgtt
     1681 gagtaacact gtgccaccgg agaaagccga tggcgcaaaa ttccagacta cacaattctg
     1741 ataattcagc cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt
     1801 ggtaccagca tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca
     1861 ggcgcggatg cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg
     1921 tcaggctccc ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct
     1981 ggaggtaatg tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc
     2041 gttaagccag gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg
     2101 gaaccaaaaa gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg
     2161 gtggaagaca aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt
     2221 ggtgctggtg cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac
     2281 ggcgattatc atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg
     2341 tgctggcact gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct
     2401 catcaaaacc tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag
     2461 gagcgtgaat tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac
     2521 gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct
     2581 gtgtacagtg aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag
     2641 cgcacaaaaa atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa
     2701 aagacggtgg tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag
     2761 aaatcacaac gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg
     2821 acgtgtggca atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg
     2881 ggaacaaagg ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta
     2941 catgccggag taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt
     3001 tttttaatgc acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc
     3061 atagaatttg ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc
     3121 acatattttg tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc
     3181 cagcagcggc tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca
     3241 ccggataagc gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg
     3301 catgcggggt tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag
     3361 ccagtatctg gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc
     3421 aaaaaatgaa actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg
     3481 atgcatcggc ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg
     3541 gcatgacaca gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta
     3601 gtatgaatga cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg
     3661 atcgcgtggc ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta
     3721 tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct
     3781 gctgtcacgg aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZOZY010000284         3797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZOZY010000284 DAZOZY010000000
VERSION     DAZOZY010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.287"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..81
                     /locus_tag="ACHURQ_004059"
     CDS             <1..81
                     /locus_tag="ACHURQ_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825696.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHURQ_004060"
     CDS             complement(98..907)
                     /gene="proC"
                     /locus_tag="ACHURQ_004060"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR0825697.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1027..1485
                     /locus_tag="ACHURQ_004061"
     CDS             1027..1485
                     /locus_tag="ACHURQ_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR0825698.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1668..2192
                     /gene="aroL"
                     /locus_tag="ACHURQ_004062"
     CDS             1668..2192
                     /gene="aroL"
                     /locus_tag="ACHURQ_004062"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR0825699.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHURQ_004063"
     CDS             2242..2433
                     /gene="yaiA"
                     /locus_tag="ACHURQ_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR0825700.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2691..3368
                     /locus_tag="ACHURQ_004064"
     CDS             2691..3368
                     /locus_tag="ACHURQ_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR0825701.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHURQ_004065"
     CDS             3440..3724
                     /gene="ppnP"
                     /locus_tag="ACHURQ_004065"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR0825702.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          903 c          990 g          953 t
ORIGIN      
        1 cgtgagtggt tgaaatcggc agatttggcg ctttacaaag caaagaaagc cggacgtaac
       61 cgcaccgaag tggcggcctg acgtccggcg aaagtcatca ggatttgctg agtttttctg
      121 atttttccat acacttcgtc atcgcttcga tcactgcggc acggaagcct ttctcttcca
      181 gtacgcgtac cgcttcaatg gtggtgcctc ccggtgagca gaccatatct ttcagtgccc
      241 ccggatgttc tcccgtttcc agcaccattt ttgcggaacc cattaccgcc tgagcggcaa
      301 atttatacgc ctgggcgcgt ggcatcccgc ccagcacggc ggcgtcggcc atcgcttcga
      361 taaacataaa tacgtaggct ggcgaagaac cgctcacacc gaccaccggg tggatcatcg
      421 gctcagcaat tacttccgct tcgccaaagc agcggaaaat attcagcaca tcagcggtat
      481 cttcgggggt taccagcgcg tttggcgtta cggaggtcat cccggcatta accagcgcgg
      541 gagtgttcgg catggcgcgg ataattttcc ggtcatggcc cagcgcgcgg gcaagctggt
      601 cgagcgtgac acctgcagca atagaaacga ccagagagtc tttattcagg ctggaggtga
      661 tttcgctaag cactttaatc atgatgccag gtttaacggc ggcaaaaatg atgtcggcga
      721 tttgcgccac ttcttgcgcc gattctgcgg cgttgatgcc gaactggtca tgcagggcgg
      781 cgactttatc cggggagggg gtgtataccc agatttgccc tggaagcacc tgaccgctgg
      841 caatcagacc gccgagaatg gcttttccca tattgccgca gccaataaaa ccgattttct
      901 tttccattgc ctcactcctg ccgtgaaatt cattgttttg ataatcgctg gcagaagcat
      961 aaacagaact atgccggaag gcaaaagcgc gacacaatag aggattaccc aacaaaggat
     1021 gactttatga caatttgggt ggatgccgac gcgtgtccca atgtaattaa agagattttg
     1081 tatcgcgcgg cggaacgtat gcagatgccg ctggtactgg tagcaaacca gagtttacgc
     1141 gtgccgccat cgcgatttat tcgtacgctg cgcgtcgcgg caggtttcga cgttgccgat
     1201 aacgaaattg tccggcagtg tgaagcgggc gatttggtga tcaccgcaga tatacctttg
     1261 gctgctgaag ccatcgagaa aggcgcagcg gcgcttaatc cgcgcggcga acgttacacg
     1321 ccagcgacca ttcgtgagcg cctgacgatg cgcgatttta tggatacctt acgtgccagt
     1381 gggatccaga ccggcggacc agatagcctt tcacaacgtg accgccaggc ctttgccgcg
     1441 gagctggaga agtggtggct ggaagtgcaa cgtagtcgtg gctaaatgta atttattatt
     1501 tacacttcat tcttgaatat ttattggtat agtaagggat gtattgagat tttcacttta
     1561 agtggaattt tttctttaca atcgaaattg tactagtttg atggtatgat cgctattctc
     1621 atgacaccgg ctttcgccgc attgcgacct attggggaaa acccacgatg acacaacctc
     1681 tttttctgat cgggcctcgg ggctgtggta aaacaacggt cggaatggcc cttgccgatt
     1741 cgcttaaccg tcggtttgtc gataccgatc agtggttgca atcacagctc aatatgacgg
     1801 tcgcggagat cgtcgaaagg gaagagtggg cgggatttcg cgccagagaa acggcggcgc
     1861 tggaagcggt aactgcgcca tccaccgtta tcgctacagg cggcggcatt attctgacgg
     1921 aatttaatcg tcacttcatg caaaataacg ggatcgtggt ttatttgtgt gcgccagtat
     1981 cagtcctggt taaccgactg caagctgcac cggaagaaga tttacggcca accttaacgg
     2041 gaaaaccgct gagcgaagaa gttcaggaag tgctggaaga acgcgatgcg ctatatcgcg
     2101 aagttgcgca tattatcatc gacgcaacaa acgaacccag ccaggtgatt tctgaaattc
     2161 gtagcgccct ggcacagacg atcaattgtt gattttagag cgcctatact taacgttcat
     2221 cccgtgaaat aaggaagaac gatgccaacg aaaccgcctt atcctcgtga agcatatata
     2281 gtgacgattg aaaaaggaaa gccaggacag acggtaacct ggtaccaact cagagccgat
     2341 catcctaaac cagactcgtt gatcagtgaa catccgacgg ctcaggaagc gatggatgcg
     2401 aaaaaacgct atgaggaccc tgacaaagag tgaccgcatc agactgctcg gaagggattc
     2461 tgagtgccac tacaagggat ctgcgtcaca tttttcataa ttcatgtttt tctaataatt
     2521 agaatattaa acaataacaa tccattactg gaatcatttg gaatctttac attatgccgt
     2581 gcacgtctgc tgctacgctt tttgtcattt gtagcacaag taagtgtcag cagtggtgct
     2641 tcacacttgc ccggtaatta acgacgaaag aaaagtaagg tggatgaaca atgagtgcgt
     2701 cgttggcgat cctgaccatc ggcattgtac ctatgcagga ggttttgccg ctcctgacgg
     2761 aatacattga cgaagataat atttcccatc atagcctgct ggggaagtta agtcgtgaag
     2821 aagtgatggc ggagtacgcg ccagaagcag gcgaagacac cattctcaca ttattaaatg
     2881 acaaccagct ggcccatgtt tcgcgtcgta aagtggagcg agacctgcaa ggtgtggttg
     2941 aagtgctcga taatcagggt tatgacgtca ttttattaat gagtacagca aacattagta
     3001 gtatgactgc gcgtaatacg atctttcttg agccgtcgcg aatattgcct ccactggttt
     3061 cctctattgt tgaagatcat caggtggggg ttatcgttcc ggttgaggag atgctgaccg
     3121 ttcaggcgca aaaatggcaa attttgcaga aatcgccggt attttcattg ggtaacccca
     3181 ttcatgattc agaacaaaaa atcattgatg ccgggaaaga attactggca aaaggggctg
     3241 atgtcatcat gctggattgt ttaggatttc accaacgtca tcgcgattta ctgcaaaaac
     3301 agctcgatgt tcctgtcttg ctgtctaacg tattgattgc acggctggct gcggaattac
     3361 tggtgtaatt ttgcgtgaca gccagcgcct ctggccccta tagtgaagta gatgttcaac
     3421 taccagacag ggccagttta tgcttcaaag taatgagtac ttttccggca aagtgaaatc
     3481 aatcggcttt tccagcagca gcactggtcg cgccagcgtg ggtgttatgg ttgaaggcga
     3541 atacaccttc agcaccgctg agccggaaga gatgacggta atcagtggcg cgctgaatgt
     3601 gttactgcct gacgcgaccg actggcaggt gtatgaagcc ggttcggtgt ttaatgttcc
     3661 cggtcacagt gagtttcatc tgcaagttgc cgaacccacc tcttatctgt gccgctatct
     3721 gtaattcctc gccttcccct tgaacgggag ggcatttttc tgaaatatcc tctctttaac
     3781 ccataataat atttcct
//
LOCUS       DAZOZY010000285         3734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZOZY010000285 DAZOZY010000000
VERSION     DAZOZY010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.288"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..182)
                     /locus_tag="ACHURQ_004066"
     CDS             complement(<1..182)
                     /locus_tag="ACHURQ_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR0825703.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="ACHURQ_004067"
     CDS             426..683
                     /locus_tag="ACHURQ_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR0825704.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHURQ_004068"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="ACHURQ_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR0825705.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHURQ_004069"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="ACHURQ_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR0825706.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHURQ_004070"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="ACHURQ_004070"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR0825707.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3734)
                     /locus_tag="ACHURQ_004071"
     CDS             complement(3308..>3734)
                     /locus_tag="ACHURQ_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR0825708.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          952 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaat
//
LOCUS       DAZOZY010000286         3731 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZOZY010000286 DAZOZY010000000
VERSION     DAZOZY010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.289"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHURQ_004072"
     CDS             <1..185
                     /locus_tag="ACHURQ_004072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825709.1"
                     /translation="KKVTLKERKHHVVTARLNDDQVLVLNQLISSGKVQKDSLSACVQ
                     YLINQYMILQGGGNEE"
     gene            175..477
                     /locus_tag="ACHURQ_004073"
     CDS             175..477
                     /locus_tag="ACHURQ_004073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825710.1"
                     /translation="MKNDFDLVQYFDDYTIVGDMDYHYFYCVKYQEGSLEAHYERIGD
                     PEVLGTITWNGNYLIDGLPMVMFVLAAEDEHKECLNRLLIVCEKARDEYRLNATGL"
     gene            532..1086
                     /locus_tag="ACHURQ_004074"
     CDS             532..1086
                     /locus_tag="ACHURQ_004074"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0825711.1"
                     /translation="MNPKITWRLIPNSKHYYVSENGDVYNDKTKQYIATKNPSNGYKK
                     VSINGKSLYVHVLVCTLFNGAKPIGGRYTVDHIDRDKMNNKAKNLRWVTQKVNNSNRK
                     HKGTRTQKLTLKQWLMIVGEYLKGQLSQYAITKWFLNQTGINICPQNIGKLFKGTTYS
                     KWYTTYLTNKQRQRIQSLTIQRKP"
     gene            1208..1669
                     /locus_tag="ACHURQ_004075"
     CDS             1208..1669
                     /locus_tag="ACHURQ_004075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825712.1"
                     /translation="MAKTYSTFDNRNIKALISQYKLYKDIPCEDIKNIMKDSVAKLKV
                     RDKISVYDVFAVLSNEDELRNDLLFNTINAIKVQQNKKQVKERMIRYYGAAITDAAKR
                     IEQYMLAHPNALPAPVYGQRTLTYKEFGKLLELQKQGDFIDDMIEYLKGLR"
     gene            2133..2876
                     /locus_tag="ACHURQ_004076"
     CDS             2133..2876
                     /locus_tag="ACHURQ_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR0825713.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            2959..3432
                     /locus_tag="ACHURQ_004077"
     CDS             2959..3432
                     /locus_tag="ACHURQ_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825714.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHKIPNSHRLIALLKQDKKT"
BASE COUNT         1320 a          611 c          753 g         1047 t
ORIGIN      
        1 tgaaaaaagt aactttaaag gaacgcaaac atcatgtagt aaccgcacgt cttaatgacg
       61 atcaagtact tgtacttaac cagctaatta gtagtgggaa ggtacagaaa gattcattat
      121 cagcctgtgt tcagtactta attaatcagt acatgatttt acagggcggt ggtaatgaag
      181 aatgactttg atttagtaca atattttgat gattacacaa tagtagggga tatggattat
      241 cactatttct attgtgtgaa gtatcaggaa ggttcattag aagcacatta tgaacgaatt
      301 ggtgatccag aagtattagg tactattaca tggaatggaa attacttaat cgacggatta
      361 ccaatggtaa tgtttgtact ggctgctgaa gatgaacata aggaatgttt aaaccgtttg
      421 ctaatcgtat gtgagaaggc gagggatgaa tataggctaa atgctactgg cctctaagta
      481 gtagcagacg cgatcacatg gagtgacgcg ataacaacaa ggacgtttga tatgaaccct
      541 aaaatcactt ggagattaat tccaaattca aaacattact acgtatctga aaatggcgat
      601 gtttacaatg ataaaacaaa acagtatata gctacgaaaa acccatctaa tggatacaaa
      661 aaagtttcta ttaatggcaa atcactatat gtacatgttt tggtttgtac tttattcaat
      721 ggtgctaaac ccattggagg aagatacact gtagatcata tcgaccgcga taaaatgaat
      781 aacaaagcaa aaaatttacg ttgggtaact cagaaggtta ataattccaa tagaaagcat
      841 aaaggtactc gtactcaaaa gcttactcta aagcaatggt taatgattgt tggagagtac
      901 cttaaaggac aattatctca atatgcaatc actaaatggt ttttaaatca aactggtatt
      961 aatatttgcc ctcagaatat aggtaagtta ttcaaaggta ctacctattc aaaatggtac
     1021 acaacatatc ttacgaataa gcaaagacaa agaatacaaa gcctaaccat tcaacgtaaa
     1081 ccataaaaaa tgccagtatt ccagtactgg catctattca aaactcaatg gcaatgaagc
     1141 caaacttatt aaatggcctg taatcactac gggtcacgat taattaattt atacaggatt
     1201 atttatcatg gcaaaaacct attcaacttt tgataacaga aacataaaag cactaattag
     1261 ccagtacaag ctatataaag atatcccatg cgaagacatc aagaacatca tgaaggactc
     1321 agtagctaaa cttaaagtga gagataagat tagtgtatat gatgtgtttg cagtattgag
     1381 taatgaagat gaattacgga atgatttact attcaatact atcaatgcta ttaaagtaca
     1441 acagaataag aaacaagtaa aagaacgtat gatcaggtac tatggggcag cgatcacgga
     1501 tgcagcaaaa cgaatagagc agtatatgtt ggcacaccca aacgcactgc cagcaccagt
     1561 atatggacaa agaacactaa catataaaga gtttggtaaa ctattggaac tacaaaaaca
     1621 aggtgacttt attgatgaca tgatcgagta cttaaagggc ttgcgttaat tgtaacttat
     1681 tacggcatat tacggcatgg atgtatgccg taatacggca aaaaaagccc ctgaaacccg
     1741 ctatttacca tcaatacggc aactaagtat agagatacga aatccattcc atttcattcc
     1801 atgtatttct ttctctatca gtattccaag aaactgtgac ttgcgtcacg aatactactc
     1861 gtcgtgaact cctcgtagta ttggttagta acgttgcgtt tttctcgcca gtactcgaaa
     1921 acccgcaagg cgttactact cgctttgctc gatagatgtt agtacagcaa atgaagtacc
     1981 taaagtgaat tggtaagtac gacatgcccc gccagttcca caactctcgc tctggtcatt
     2041 tccactacta tgatgctatt ggatagctac ataaagtact ggatcaagta ggcatttttt
     2101 gtgtacaaag agggaattaa gagagggata ccatgaacag aaaacaattc atccagtcgc
     2161 atggtgctac ttgtagtaac tggacttgga gttggtcatt tgtgaaccac gacaagaaaa
     2221 tggttatctt tggtgcatgg gatgtacaaa aagagcaaga aagatcagtc attctgaggg
     2281 agcggtggaa aaccaaagtt gtggagggaa aggaaagaaa aaatcttgga tatattcaag
     2341 ctattgaaca cattcaattg atagctgaag gttatgagct ttacacgttt gatatggagc
     2401 agggacgaaa cgatgagaat agagatgtag ctgttatcaa acggttcact ccaaaacttg
     2461 aaaaacgtta ccttcggaaa gaaggaacag tttggtatgc agacttttta cctaatccat
     2521 ttccagatga aattacctca cctgaaaact acgctgaggg tgcaaaaaag caagttacag
     2581 taaactccta cgagcgtgat ccaaaagcac gacaggcttg tattgaccat catggtactt
     2641 cctgcgggtg ttgtgagttt aactttgaga aagtctatgg tgaacacgcc aagggcttta
     2701 tacacgttca tcatatcaaa ccgctacaca cagtaggcga ggattacgag gtaaatccaa
     2761 ttactgatat ggttccactt tgtccaaact gtcacgcaat ggtacatcgc ggtaacgaag
     2821 tattaagtgt cgaaagttta aaagcactgt tgtttaaaga cagcaataaa atttgaattt
     2881 attttagact tgatacgaca tacttgaaaa gttatgaata caatatgctt taaattagac
     2941 atgtaagttg agagaaaaat gagcataaca acaatagcag ctacatataa agatgtgtta
     3001 actattgctt tttcggcgtt gtcatttatt attgcatgta cttccttata cttctccggt
     3061 agggcagcat ggcatgatag atcaaggcta aaaataacag ctgacctcgt ctacgagtct
     3121 tttgatgagg aaccttataa aatcgaggtg atagtgatca atgttggtag gcgtgatgca
     3181 gttttggaag ggatgctttg ccattatgaa aaaggcatta ggagccattc atctccaaaa
     3241 gagagcattg ttattaaaga aaagcaaaga aaaaaatttg aattggtaag ctcagactta
     3301 gtcacgatag ataatgaatg tgatatttgt catttagaag atataactat tttagatgtg
     3361 gaggggaatg aacataaaat ccctaactct cacagactca ttgcattact taagcaagat
     3421 aaaaagactt aagaataata ttttttggaa tgtaaggtaa atgttagatg ccactataga
     3481 ttgagggtta gaactactaa cccactatag ggtttacgag atgataagaa tggaatttaa
     3541 cacctacgat ggtgatatat attacttcct ctatttatat aggaggtacg aaagggggag
     3601 tactttaatg aagaccttat aaatcttaat gttgagatgt tagttatagc ggtgaggata
     3661 ccccaccgtc tattgggagt tgtggactct aattagagaa aacgaataag ttattcatgt
     3721 cctttttcaa g
//
LOCUS       DAZOZY010000287         3666 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZOZY010000287 DAZOZY010000000
VERSION     DAZOZY010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.290"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..467)
                     /locus_tag="ACHURQ_004078"
     CDS             complement(<1..467)
                     /locus_tag="ACHURQ_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR0825715.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKK"
     gene            complement(514..1233)
                     /gene="btsR"
                     /locus_tag="ACHURQ_004079"
                     /gene_synonym="yehT"
     CDS             complement(514..1233)
                     /gene="btsR"
                     /locus_tag="ACHURQ_004079"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR0825716.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1230..2915)
                     /gene="btsS"
                     /locus_tag="ACHURQ_004080"
     CDS             complement(1230..2915)
                     /gene="btsS"
                     /locus_tag="ACHURQ_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR0825717.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3137..3202
                     /locus_tag="ACHURQ_004081"
                     /pseudo
     CDS             3137..3202
                     /locus_tag="ACHURQ_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3430..>3666
                     /locus_tag="ACHURQ_004082"
     CDS             3430..>3666
                     /locus_tag="ACHURQ_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR0825718.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT          978 a          948 c          846 g          894 t
ORIGIN      
        1 cttttcttca catgctggcg cgctgccagt ccacgcagaa aataacgtaa aaattgatcg
       61 ccgcattcgc ggaagttttt atgatccggt gcgcgcatca tcgctgtaat ttccggcatc
      121 gaaacgcgga actgctgttc ggtgaggatc gccagaatgt catcggtttt cagcgaaaac
      181 gcgatgcgta attttttcag cacgatgttg ttattaatgc gacgttccgg ctccagtgcc
      241 ggagcagact catccttgcc gcgtttttca taaatcaggc cattgaggaa tgacgacaaa
      301 acaatgtccg gacaacgctg aaaaccctct tcgtcttctt tacgtagcca gacggcgatc
      361 tgttccgcgg tggcttcgac attacccagc gccagaatac gcaccaggtc attattattg
      421 gctttcaaaa tgtagcgcac gctgcgcaga atatcgttac ttagcatgag gccttcaggt
      481 gttgatgagg caaaaagcca ttttagcagt cttttacagg ccaatcgcct cttttaagct
      541 tttcagataa cggcggctga ccggcacggt taagccatta cgcaaaatca actcggcctg
      601 gccgttatct tccagacgaa tctcctgtaa atgcgcgagg ttaaccagat actgacgatg
      661 gcagcgcagt agtggtgtac gactttccag ggtacgtaat gtcaattcgg taaagccctc
      721 tttcccttcg tggctggtaa cgtagacacc gctcatccga ctgctgacaa atgccacatc
      781 tttcatttgc agcaaataaa tccgactatg ccccgtacaa gggataaatt tcagcgcctg
      841 ttgattttcc ggtaacagcg aaacatcctg cttgctgcgc tcctgacgca atcgcgccag
      901 cgttttctcc agtcgcgctt catcaattgg cttcagcaga taatcaaagg catgttcttc
      961 aaaggcttta atagcgtatt cgtcaaacgc agtgagaaaa acaatatacg ggcgatgttc
     1021 cgggtcaagc atccccacca tttccagacc actgatgcgc ggcatctgga tatcgagaaa
     1081 cagcacatcc gggcgcagtt tatgcaccgc gccgatccct tccacggcgt ttgaacactc
     1141 tccaacgatt tcaatatcgc tctgctcctg caaaaataca cgcaggttct cccgtgctaa
     1201 cggttcatca tcgacaatta agactttaat catgcctcgt ccctccatgg tagtcgtaac
     1261 gttattcggg tgtaactatc aggctcacag gcgacgctta ttccatagtc atcgccaaac
     1321 cgttcacgta aacgcttatc caccagattc atccccagcc cactggcatt ggttaccggt
     1381 tgatacaaac cggcattgtc ttcgatctcc agcatcaaat gttgcccctc acgtcgggcg
     1441 ctgattgcca ctcgccctgt atccagcagt tgtgatgtcc catgtttaat ggcgttttcc
     1501 actatcggtt gcagggtaaa cgcgggcaat tgctgctggg ataattcttg cggaatagca
     1561 atgttgacct gcaaccgcga ctggaagcgc gccttttcaa tttgcagata agcgttcaca
     1621 tgttcaattt cgtcggcgag agtaacaaac tccgaaggcc gctttaagtt tttgcggaaa
     1681 aaagtggaaa gatactgcac cagctggctg gcctgttcgc tgtcgcggcg gatcaccgct
     1741 ttaatggtgt taagcgcatt aaacaaaaaa tgaggattca cctgggcgtg aagcagtttg
     1801 atttctgact gggtgagcat cgctttttgc cgctcatatt gcccggcaag aatctgcgcc
     1861 gaaagcagtt gcgcaatccc ctcgcccagc gtgcggttta ttgaactgaa taaacggttt
     1921 ttggcttcat acaatttgat ggtgcccatc acgcgctgat tttcgccacg caacggaatt
     1981 accagcgtcg accccagttt gcattgcgga tgcaaagagc aacgataagg tacttcgttg
     2041 ccatcagcgt agaccacttc accggtttca atcgctttta aggtgtaagg cgaagaaatc
     2101 ggtttgccag gtaaatggtg gtcgtcacca attccggtaa aggccagcaa tttctctcga
     2161 tcggtaatcg cgactgcacc aatatccagc tcctgataca gcacctgagc cactttcatg
     2221 ctgttcactt cgttaaaccc ctgtcgcaaa atgccttccg tcgaggctgc cactttcagc
     2281 gcagtggcag aaaaagccga agtgtatttt tcaaacatcg cgcgtttatc gagcaatata
     2341 cgcataaaca gcgccgcgcc gacggtattg gtgaccatca ttggcgcagc aatattactc
     2401 accagacgca ccgcatcttc ataaggtcgg gcgatcgcca ggatgatcag catttgcacc
     2461 atttcagcga cgaacgtgac ggcaccggcg gtaatggggt taaagacttt atcagtgcgc
     2521 ccgcgacgga tcaggatgct gtgtaccagg ccgccgagca atccttcaac gatggttgag
     2581 atcatgcaac ttagcgcggt catgcccccc atcgaatatc gatgtaagcc gccggtcaga
     2641 ccaaccagcc caccgacgac cggaccgccg agtaagccgc ccattaccgc gcctatcgca
     2701 cgggtattgg caatagaatc gtcaatgtgc aacccaaacc aggtgcccat gatgcagaag
     2761 atggaaaaga cgatgtagca gagaaattta tgcggcagac gaaccgtgac ctgcattaac
     2821 ggtatgaata atggcgtttt actcattaac cacgcaatga ctaaaaaaac gcacatctgc
     2881 tgaagcagca gcaacaccag attaaaatcg tacatacccg caaaccacac ttccctttaa
     2941 aacgcgtaac atacattgcc tgcgtttaac tttctttgaa ctcttgcaga aaaatgagaa
     3001 ttcgtgagta cgatcactca aaatcgcctg gcaaaaataa aatcacccta tagatgcaca
     3061 aaaaacgggc aaaactacct ggttcgcaaa actgcgtcta aagttaaacc gggacctcgc
     3121 gagcaagggt gagacgatgg cgctttacac aattggtgaa gtggcgttgc tttgtgatat
     3181 taaccctgtc acgttacgcg cgtgacgcaa catttttaaa aatcgacgaa gatcgtccag
     3241 gagcgccgtt tttcaagggt tggatagatg attcgtttac catgtcgacg aacttcgacg
     3301 aatttcgccg ataaaaagtg tcccatacat gccccattga tgccccataa acaatatcag
     3361 gaattgacac tggttatcca tacagataaa aataacacct gtacataacc acagttacag
     3421 gagggtgtta tgcgtgttga gatctgtatc gctaaagaaa aaatcactaa aatgccaaac
     3481 ggtgctgtgg atgcgttaaa ggaagaatta acccgacgca tcagtaaacg ttatgacgat
     3541 gtagaggtga tcgtaaaagc caccagcaac gatggccttt ctgttacacg caccgcagat
     3601 aaggattctg caaaaacatt tgttcaggag actctgaaag atacctggga gtctgctgac
     3661 gagtgg
//
LOCUS       DAZOZY010000288         3663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZOZY010000288 DAZOZY010000000
VERSION     DAZOZY010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.291"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(56..100)
                     /locus_tag="ACHURQ_004083"
     CDS             complement(56..100)
                     /locus_tag="ACHURQ_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825719.1"
                     /translation="MKRNDKILHYEGLN"
     gene            219..1943
                     /gene="ilvI"
                     /locus_tag="ACHURQ_004084"
     CDS             219..1943
                     /gene="ilvI"
                     /locus_tag="ACHURQ_004084"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR0825720.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            1946..2437
                     /gene="ilvN"
                     /locus_tag="ACHURQ_004085"
     CDS             1946..2437
                     /gene="ilvN"
                     /locus_tag="ACHURQ_004085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR0825721.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            2449..2535
                     /locus_tag="ACHURQ_004086"
     CDS             2449..2535
                     /locus_tag="ACHURQ_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR0825722.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            2617..3621
                     /gene="cra"
                     /locus_tag="ACHURQ_004087"
     CDS             2617..3621
                     /gene="cra"
                     /locus_tag="ACHURQ_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR0825723.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT          861 a          868 c         1012 g          922 t
ORIGIN      
        1 ttaattaaaa aaatagagaa attgctgtaa gttgtgggat tcagccgatt tattatcaat
       61 ttaatccttc gtaatggagg attttatcgt ttcttttcac ctttcctcct gtttattctt
      121 attaccctgc gtttatgtct ctggctgcca attgcttaag caagatcgga cggttaatgt
      181 gttttacaca ttttttccgt caaacagtga ggcaggccat ggagatgttg tctggagccg
      241 agatggtcgt ccgatcgctt atcgatcagg gcgttaaaca agtattcggt tatcccggag
      301 gcgcagtcct tgatatttac gatgcattgc ataccgtggg tggtatcgat catgttttag
      361 ttcgtcatga gcaggcggca gtgcatatgg ccgatggtct ggcgcgcgcg accggggaag
      421 tcggcgtcgt gctggtaacg tcgggtccag gggcgaccaa tgcgattact ggcatcgcca
      481 ccgcttatat ggattccatt ccattagttg tcctttccgg gcaggtagcg acctcgttga
      541 taggttacga tgcctttcag gagtgcgaca tggtggggat ttcgcgacca gtggttaaac
      601 acagttttct ggttaagcaa acggaagaca ttccgcaggt gctgaaaaag gctttctggc
      661 tggcggcaag tggtcgtcct ggaccagtag tcgttgattt accgaaagat attcttaatc
      721 cggcgaacaa attaccctat gtctggccgg agtcggtcag tatgcgttct tacaatccca
      781 ctactaccgg acataaaggg caaattaagc gcgctctgca aacgctggta gcggcaaaaa
      841 aaccggttgt ctacgtaggc ggtggggcaa tcacggcggg ctgccatcag cagttgaaag
      901 aaacggtgga ggcgttgaat ctgcccgttg tttcctcatt gatggggctg ggagcgtttc
      961 cggcaacgca tcgtcaggca ctgggcatgc tgggaatgca cggtacctac gaagccaata
     1021 tgacgatgca taacgcggat gtgattttcg ccgtcggggt acgatttgat gaccgaacga
     1081 cgaacaatct ggcaaagtac tgcccaaatg ccactgttct gcatatcgat attgatccta
     1141 cttccatttc taaaaccgtg actgcggata tcccgattgt gggggatgct cgccaggtcc
     1201 tcgaacaaat gcttgaactc ttgtcgcaag aatccgccca tcaaccactg gatgagatcc
     1261 gcgactggtg gcagcaaatt gaacagtggc gcgctcgtca gtgcctgaaa tatgacactc
     1321 acagtgaaaa gattaaaccg caggcggtga tcgagactct ttggcggttg acgaagggag
     1381 acgcttacgt gacgtccgat gtcgggcagc accagatgtt tgctgcactt tattatccat
     1441 tcgacaaacc gcgtcgctgg atcaattccg gtggcctcgg cacgatgggt tttggtttac
     1501 ctgcggcact gggcgtcaaa atggcgttgc cagaagaaac cgtggtttgc gtcactggcg
     1561 acggcagtat tcagatgaac atccaggaac tgtctaccgc gttgcaatac gagttgcccg
     1621 tactggtggt gaatctcaat aaccgctatc tggggatggt gaagcagtgg caggacatga
     1681 tctattccgg ccgtcattca caatcttata tgcaatcgct acccgatttc gtccgtctgg
     1741 cggaagccta tggtcacgtc gggatccaga tttctcatcc gcatgagctg gaaagcaaac
     1801 ttagcgaggc gctggaacag gtgcacaata atcgcctggt gtttgttgat gttaccgtcg
     1861 atggcagcga gcacgtctac ccgatgcaga ttcgcggggg cggaatggat gaaatgtggt
     1921 taagcaaaac ggagagaacc tgattatgcg ccggatatta tcagtcttac tcgaaaatga
     1981 atcaggcgcg ttatcccgcg tgattggcct tttttcccag cgtggctaca acattgaaag
     2041 cctgaccgtt gcgtcaaccg atgatccgac attatcgcgt atgaccatcc agaccgtggg
     2101 cgatgaaaaa gtacttgagc agatcgaaaa gcaattacac aagctggtcg atgtcttgcg
     2161 cgtgagtgag ttggggcagg gcgcgcatgt tgagcgggaa atcatgctgg tgaaaattca
     2221 ggccagcggt tacgggcgtg acgaagtgaa acgtaatacg gaaatattcc gtgggcaaat
     2281 tatcgatgtc acaccctcgc tttataccgt tcaattagca ggcaccagcg ataagcttga
     2341 tgcattttta gcatcgattc gcgaggtggc gaaaattgtg gaagttgctc gttctggtgt
     2401 ggtcggactt tcgcgcggcg ataaaataat gcgttgagaa tgatctgaat gcgcaatttg
     2461 cagcccaaca tgtcacgttg ggcttttttt gcgaaatcag tgggaacctg gaataaaagc
     2521 agttgccgca gttaatttta tgcgcttaga tgttaatgaa tttaacccat accagtacaa
     2581 tggctatggt ttttacattt tacgcaaggg gcaattgtga aactggatga aatcgctcgg
     2641 ctggcgggag tgtcgcggac cactgcaagc tatgttatta acggcaaagc gaagcaatac
     2701 cgtgtgagcg acaaaaccgt tgaaaaagtc atggctgtgg tgcgtgagca caattaccac
     2761 ccgaacgccg tggcagctgg gcttcgtgct ggacgcacac gttctattgg tcttgtgatc
     2821 cccgatctgg agaacaccag ctatacccgc atcgctaact atcttgaacg ccaggcgcgg
     2881 caacggggtt atcaactgct aattgcctgc tcagaagatc agccagacaa cgaaatgcgc
     2941 tgtattgagc accttttaca gcgtcaggtc gatgccatta ttgtttcgac gtcgttgcct
     3001 cctgagcatc ctttttatca acgctgggct aacgacccgt tcccgattgt cgcgctggac
     3061 cgcgccctcg atcgtgaaca cttcaccagc gtggttggtg ccgatcagga tgatgccgaa
     3121 atgctggcgg aagagttacg taagtttccc gccgagacgg tgctttatct tggcgcgctg
     3181 ccggagcttt ctgtcagctt cctgcgtgaa caaggtttcc gtactgcctg gaaagatgat
     3241 ccgcgcgaag tgcatttcct gtatgccaac agctatgagc gggaggtggc tgcccagtta
     3301 ttcgaaaaat ggctggaaac gcatccgatg ccgcaggcgc tgttcacaac gtcgtttgcg
     3361 ttgttgcaag gggtgatgga tgtcacgcta cgtcgcgatg gtaaactgcc ttctgacctg
     3421 gcaattgcca cctttggcga taacgaactg ctcgacttct tacagtgccc ggtgctggca
     3481 gtggctcaac gtcaccgcga tgtcgcagag cgtgtgctgg agattgtcct ggcaagcctg
     3541 gacgaaccgc gtaagccaaa acctggttta acgcgcatta aacgtaatct ctatcgccgc
     3601 ggtgtgctca gccgtagcta agccgcgaac aaaaatacgc gccaggtgaa tttccctctg
     3661 gcg
//
LOCUS       DAZOZY010000289         3659 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZOZY010000289 DAZOZY010000000
VERSION     DAZOZY010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.292"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            167..1270
                     /gene="proB"
                     /locus_tag="ACHURQ_004088"
     CDS             167..1270
                     /gene="proB"
                     /locus_tag="ACHURQ_004088"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR0825724.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1282..2535
                     /gene="proA"
                     /locus_tag="ACHURQ_004089"
     CDS             1282..2535
                     /gene="proA"
                     /locus_tag="ACHURQ_004089"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR0825725.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2650..2725
                     /locus_tag="ACHURQ_004090"
     tRNA            2650..2725
                     /locus_tag="ACHURQ_004090"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2683..2685,aa:Thr,seq:cgt)
     gene            3331..3489
                     /locus_tag="ACHURQ_004091"
     CDS             3331..3489
                     /locus_tag="ACHURQ_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825726.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT          926 a          873 c          981 g          879 t
ORIGIN      
        1 ggtaatttat taaatctgta ataaaagcgt aaacaactgc cgctaggctt gctgatcccg
       61 cgcaacaaaa cgccatgctt tgctcgcaga tggttggcaa ccgacgacag tcctgctaaa
      121 acgttcgttt gatatcattt ttcctaaaat tgaatggcag agaatcatga gtgacagcca
      181 gacgctggtg gtaaaactcg gcaccagtgt gctaacaggc ggatcgcgcc gtctgaaccg
      241 tgcccatatt gttgaacttg ttcgccagtg cgcgcagtta catgccgccg ggcatcggat
      301 tgttattgtg acgtcgggcg cgatcgccgc cggacgtgag cacctgggtt acccggaact
      361 gccagcgact atcgcctcga aacaactgct ggcagcggta gggcagagtc gactgattca
      421 actgtgggaa cagctgtttt cgatttatgg cattcacgtc gggcaaatgc tgctgacccg
      481 tgctgatatg gaagaccgtg aacgcttcct gaacgcccgc gacaccctgc gtgcgttgct
      541 cgataacaat atcgttccgg taatcaatga gaacgatgct gtcgctacgg cagagattaa
      601 agtcggcgat aacgataacc tttctgcgct ggcggcgatt ctggcgggtg ccgataaact
      661 gttgctgctg accgatcaaa aaggtttgta taccgctgac ccgcgcagca atccgcaggc
      721 agaactgatt aaagatgttt acggcattga tgacgcactg cgcgcgattg ccggtgacag
      781 cgtttcaggc ctcggaactg gcggcatgag taccaaattg caggccgctg acgtggcttg
      841 ccgtgcgggt atcgacacca ttattgccgc gggcagcaag ccgggcgtta ttggtgatgt
      901 gatggaaggc atttccgtcg gtacgctgtt ccatgcccag gcgactccgc ttgaaaaccg
      961 taaacgctgg attttcggtg cgccgccggc gggtgaaatc acggtagatg aaggggcaac
     1021 tgccgccatt ctggaacgcg gcagctccct gttgccgaaa ggcattaaaa gcgtgactgg
     1081 caatttctcg cgtggtgaag tcatccgcat ttgcaacctc gaaggccgcg atatcgccca
     1141 cggcgtcagt cgttacaaca gcgatgcatt acgccgtatt gccggacacc actcgcaaga
     1201 aattgatgca atactgggat atgaatacgg cccggttgcc gttcaccgtg atgacatgat
     1261 tacccgttaa ggagctggcc gatgctggaa caaatgggca ttgctgcgaa gcaagcctcg
     1321 tataaattag cgcaactctc cagccgcgaa aaaaatcgcg tgctggaaaa aatcgccgat
     1381 gaactggaag cacaaagcga aatcatcctc aacgctaacg cccaggatgt tgctgacgcg
     1441 cgagccaatg gccttagcga agcgatgctt gaccgtctgg cactgacgcc cgcacggctg
     1501 aaaggcattg ccgacgatgt acgtcaggtg tgcaacctcg ccgatccggt ggggcaggta
     1561 atcgatggcg gcgtactgga cagcggcctg cgtcttgagc gtcgtcgcgt accgctgggg
     1621 gttattggcg tgatttatga agcgcgcccg aacgtgacgg ttgatgtcgc ttcgctgtgc
     1681 ctgaaaaccg gtaatgcggt gatcctgcgc ggtggcaaag aaacgtgtcg cactaacgct
     1741 gcaacggtgg cggtgattca ggacgccctg aaatcctgcg gcttaccggc gggtgccgtg
     1801 caggcgattg ataatcctga ccgtgcgctg gtcagtgaaa tgctgcgtat ggataaatac
     1861 atcgacatgc tgatcccgcg tggtggcgct ggtttgcata aactgtgccg tgaacagtcg
     1921 acaatcccgg tgatcacagg tggtataggc gtatgccata tttacgttga tgaaagtgta
     1981 gagatcgctg aagcattaaa agtgatcgtc aacgcgaaaa ctcagcgtcc gagcacatgt
     2041 aatacggttg aaacgttgct ggtgaataaa aacatcgccg atagcttcct gcccgcatta
     2101 agcaaacaaa tggcggaaag cggcgtgaca ttacacgcag atgcagctgc actggcgcag
     2161 ttgcaggcag gccctgcgaa ggtggttgct gttaaagccg aagagtatga cgatgagttt
     2221 ctgtcattag atttgaacgt caaaatcgtc agcgatcttg acgatgccat cgcccatatt
     2281 cgtgaacacg gcacacaaca ctccgatgcg atcctgaccc gcgatatgcg caacgcccag
     2341 cgttttgtta acgaagtgga ttcgtccgct gtttacgtta acgcctctac gcgttttacc
     2401 gacggcggcc agtttggtct gggtgcggaa gtggcggtaa gcacacaaaa actccacgcg
     2461 cgtggcccaa tggggctgga agcactgacc acttacaagt ggatcggcat tggtgattac
     2521 accattcgtg cgtaaataaa accgggtgat gcaaaagtag ccatttgatt cacaaggcca
     2581 ttgacgcatc gcccggttag ttttaacctt gtccaccgtg attcacgttc gtgaacatgt
     2641 cctttcaggg ccgatatagc tcagttggta gagcagcgca ttcgtaatgc gaaggtcgta
     2701 ggttcgactc ctattatcgg caccattaag aaaattaata acttagctta acttctctaa
     2761 gaaattgtat cttctgttat cacttacaaa cgcctatacg ttgttatggg ctttggtagt
     2821 gtaacacgta tgggcgagta gttcccaggc tcaacctcgt aacaagttcc tttgaagctg
     2881 gaagacgtgc caccgtctct aaaccttcta ccaaaactct tagcaagatg ttccacacct
     2941 ggcgcggtgt ccaatgatgc tacacgtcgt gagacgttgc agaggataaa caggctaaat
     3001 gatgggaaga gttttaagtg caatagtgga ttaaagccca cctcttcaaa acctaacttg
     3061 atcgggaaag aatcaagctc tagcacggtt tcgtccaccg gagtacaacg ggtgcctgat
     3121 aagagaagaa ctgctaagac ttcatagctt ttgtgaaaaa gcccttttta gtggatacgc
     3181 ctacggcgct tattttttgg tataaccatc aaaggcgctt tatggctcct attgtcttga
     3241 aaatgtatct ttacttgtat tgtgttttat gcgtagtctc cctgtgtagt atgtaacttg
     3301 ttaattttca tatgaataca gaggcacacg atgaaaaaca ttgcagcaat caaacgtaat
     3361 aaccgcaaga ttcacgctcg taagttcctg tctacgccag aaggaaaagc ctggctagag
     3421 cgtaaacaga gagagaacga agaaagaaaa ctccttagtg agttgaaatg gcttagagag
     3481 gatttctgaa aatctccaga acgcaacagg acgcattcta acgctgtttt cattactcgg
     3541 tatagggata ggtgatgtgt gtttaatgcg tctgtgcgct tgttttgtgc cttattttgc
     3601 attttcataa tgcggaagag aacacggaaa accttaaaat taattattgg ggtatttaa
//
LOCUS       DAZOZY010000290         3631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZOZY010000290 DAZOZY010000000
VERSION     DAZOZY010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.293"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..938
                     /locus_tag="ACHURQ_004092"
     CDS             <1..938
                     /locus_tag="ACHURQ_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0825727.1"
                     /translation="AMLLCKWGFYLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYL
                     LIMMGLIVRAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFW
                     PRKPEPGKDTFQYHVLPFDSIDIISKRRESLENQWGIEDSESYCALMEHFLSGDHGAN
                     TFKANMEEAPEQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMTSISST
                     AFQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKLTDAEL
                     EACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(1055..2398)
                     /gene="gdhA"
                     /locus_tag="ACHURQ_004093"
     CDS             complement(1055..2398)
                     /gene="gdhA"
                     /locus_tag="ACHURQ_004093"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR0825728.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            2634..2906
                     /locus_tag="ACHURQ_004094"
                     /pseudo
     CDS             2634..2906
                     /locus_tag="ACHURQ_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            complement(2872..3279)
                     /gene="nudG"
                     /locus_tag="ACHURQ_004095"
     CDS             complement(2872..3279)
                     /gene="nudG"
                     /locus_tag="ACHURQ_004095"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR0825729.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            3366..>3631
                     /locus_tag="ACHURQ_004096"
     CDS             3366..>3631
                     /locus_tag="ACHURQ_004096"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR0825730.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
BASE COUNT          910 a          894 c          893 g          934 t
ORIGIN      
        1 aggccatgct gctttgtaag tgggggtttt atttaacatg tgttgtcgct gtaatgtttg
       61 tgttcgcagc gataacatcc aacggtttga atgaaagagg cctgattacc gcgggatgct
      121 cttttcttta tctattaatt atgatggggc ttattgttcg ggccggtttt aaagcaaaaa
      181 aagaacaact gcattattat caggctaaag gtattgagcc gctcagtatc gagaagttac
      241 aggcgctaca attgatcgca ccttatcgat tctatcataa gcaatggtct gaaacgctag
      301 agttctggcc gcgaaagcct gaacctggta aagatacctt ccaatatcat gtgcttcctt
      361 ttgactcgat cgatatcatc agtaaaagac gcgagtcttt agagaatcaa tggggtatcg
      421 aagatagcga aagttattgt gccttaatgg agcattttct ttctggcgac catggagcca
      481 atacctttaa agcaaacatg gaggaagccc cagagcaggt tatcgccttg ttgaataaat
      541 ttgctgtttt tccctcagac tatatctctg attgcgctaa taatcgctcc ggtaaatcct
      601 cggcgaagct aatatgggcg gcggaattat catggatgac ctcgatatca agcacagctt
      661 ttcaaaacgg gacaattgaa gaagaactgg cctggcatta tataatgctt gcttctcgaa
      721 aggcgcacga gttgttcgaa agcgaagaag attatcaaaa aaatagtcta atggggtttc
      781 tttactggca tatctgctgc tatcgcagaa agttaacgga tgctgaactt gaagcgtgtt
      841 atcgttacga caagcaattt tgggagcact acagtaagaa atgccgttgg cccataagaa
      901 atgttccctg gggagcatca tccgttaaat actcataaac gcctgaaatt ttgccggggg
      961 cgctgcgctt gcccggccta cattagagcg taactgcaat gaattgtgcc catttgtagg
     1021 cctgataagc gtagcgccat caggcattta caacttaaat cacaccctgc gccagcatcg
     1081 catcggcaac cttcacaaaa ccggcaatgt tcgcgccctg cacgtagttg gtttgctcac
     1141 cttcaccacc atgctcaaca caggcatggt ggatatccag catgatgtga tgcaaacgtg
     1201 cgtcaacttt ctcggctttc cagcccaggc gcgcagcgtt ttgtgccatt tccaggcccg
     1261 acgttgctac accgccagca ttagccgctt tacccggtgc aaacagtacg cctgcctgct
     1321 ggaacagttc agtcgcttcg atggtggtcg gcatatttgc cccttcagcg acggctttaa
     1381 cgccattggc gataagctga tgcgcggcgt caacatccag ttcgttctgg gtggcgcaag
     1441 gcagggcgat atcaaccggt acagaccacg gctgttggcc ttcgagatag accagaccaa
     1501 attctttggc gtaatctgcc actcgaccat cgcggctggc tttgatttcg ataagacgtg
     1561 ccagtttctc tttcgtgaat ccgctttcat caactacagt gccgctggag tctgacgcag
     1621 tgatcacacg agcaccaaat tccatcgctt tttcgatagc gtactgggcg acgttgccgg
     1681 agccagaaac ggaaacgcgc atcccttcaa aacccatacc gtggcgtttt aacattgctt
     1741 ctgtgaaata aaccagaccg tagccggtag cttccgggcg aataagacta ccgccaaatg
     1801 aaaggccctt accggtgaag acgcaggcgg tattgttgga gagctttttc atcatccccg
     1861 ccataaagcc gacttcacga ccaccaacgc cgatatcacc tgccggaacg tcggtatccg
     1921 cgcccagatg acgataaagc tcggtcatca gcgcctggca aaaacgcatc acttcacctt
     1981 cgctttttcc tttcggatcg aaatcgctgc cgcctttacc accgcccatc ggcagggtag
     2041 tcagggcatt tttgaaggtt tgttcaaagc cgaggaattt gagaatggaa aggttaactg
     2101 acggatggaa gcgcataccg cctttgtacg ggccgatggc agagctgaac tgcacacgcc
     2161 atgcacggtt gacctgtatc tggttgcgat catcaaccca taccacgcga aactggatca
     2221 cgcgctccgg ttcaaccaga cgctccagta atgacatctg gcgatatttt ggattttgtt
     2281 caagaaaagg ccagagtgtg gtcattactt cacgaacggc ttgcgcgaac tcggtttgat
     2341 tcgggtcgcg cttttggaca tggttgagga atgactccag agaatatgtc tgatccatag
     2401 atataaaacc cttatatatt aatacgattg tgcttatgtt gtttatgtca tgtgcttttg
     2461 cagttttcga ctataccatc aagaatgtga cggtaagcaa gaaaaaaatg acccgggaaa
     2521 gcaaattaat aacgagagta atctcataac caaatgcgat aactcagacg ctaaagtttt
     2581 gccacccggc taccgttatc agttgcagag acaataacgt taaggagttc cctgtgagtc
     2641 gagcattgtt cgccgtagta ttagcatttc cattaattgc actggctaac ccgcattatc
     2701 gtccggacgt ggaggtaaac gtaccgccag aagtgttcag ttcaggcggg cagtcagcgc
     2761 agccctgtac tcagtgctgc gtttatcagg atcaaaatta ttctgaaggg gcggtaatta
     2821 aagccgaagg aatactgctg taatgccagc gggatgacaa aacccttagc actaatccgc
     2881 tgctctggcg gcgcgtaaag ccataaacgc ctctaatagt ggaatgtcag caggggccag
     2941 cggatattgc agcgcctctt caggtgagca ccagaccagc gcctgatgtt catgtgcctg
     3001 taacgtcccg tggaagtcgg gtacgtgcca ggcatgaaga tggataatcc ggcccgaaac
     3061 ttctcgctga tggctggcaa catactcacc cacagttgct tcgatgccca attcttcgtt
     3121 taactcacgc accagtgcct gctgctggct ttcatccagc tcgactttac catcggcaaa
     3181 ctcccataat cccgcctgat cgctctgggc ggggcgttgc gcgagtaaaa ttttgccatc
     3241 acgttcaatg atggcggcaa caacttcaat cattttcata aattacacac gggcagataa
     3301 gcgaaaaatg ccataatatc cgcactgttg ctcaatcaga aaggctttat atttaaggag
     3361 tgcctgtgct agaccgccat cttcatcccc ggattaaacc gttgttgcat cagtgcgtgc
     3421 gggttcttga taaaccgggc attacgccag atggtttaac attagtggga tttgccatcg
     3481 gcgtgctggc tctgccgttt ctggcgctgg gctggtatct ggcggcgcta gtcgtcattt
     3541 tgttgaacag gctgcttgat ggtctggacg gtgcgctggc gcggcgcaga gagcttaccg
     3601 acgcgggcgg ctttcttgat atttctcttg a
//
LOCUS       DAZOZY010000291         3627 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZOZY010000291 DAZOZY010000000
VERSION     DAZOZY010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.294"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(89..652)
                     /locus_tag="ACHURQ_004097"
     CDS             complement(89..652)
                     /locus_tag="ACHURQ_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR0825731.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="ACHURQ_004098"
     CDS             complement(756..974)
                     /locus_tag="ACHURQ_004098"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825732.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="ACHURQ_004099"
     CDS             complement(1133..1519)
                     /locus_tag="ACHURQ_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR0825733.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="ACHURQ_004100"
     CDS             complement(1509..1880)
                     /locus_tag="ACHURQ_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0825734.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="ACHURQ_004101"
     CDS             complement(1880..2137)
                     /locus_tag="ACHURQ_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825735.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="ACHURQ_004102"
     CDS             complement(2134..3525)
                     /locus_tag="ACHURQ_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR0825736.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="ACHURQ_004103"
     CDS             complement(3522..>3627)
                     /locus_tag="ACHURQ_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0825737.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAZOZY010000292         3560 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZOZY010000292 DAZOZY010000000
VERSION     DAZOZY010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.295"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            129..1376
                     /locus_tag="ACHURQ_004104"
     CDS             129..1376
                     /locus_tag="ACHURQ_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR0825738.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHURQ_004105"
     CDS             complement(1905..2105)
                     /gene="torI"
                     /locus_tag="ACHURQ_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR0825739.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2228..2572)
                     /locus_tag="ACHURQ_004106"
     CDS             complement(2228..2572)
                     /locus_tag="ACHURQ_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825740.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2814..3416
                     /locus_tag="ACHURQ_004107"
     CDS             2814..3416
                     /locus_tag="ACHURQ_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825741.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          887 a          715 c          816 g         1142 t
ORIGIN      
        1 gcagtaacgg cgaaaggggc aatgacagcg ctgccatgtc gacaagaact ggaatagtga
       61 gaagtaaacg gcgaagtcgc tcttatctct aaataggacg tgaatttttt aacgacaggt
      121 aggtaattat ggcactgaat attccattca gaaatgcgta ctatcgtttt gcatccagtt
      181 actcatttct cttttttatt tcctggtcgc tgtggtggtc gttatacgct atttggctga
      241 aaggacatct aggattgaca gggacggaat taggtacact ttattcggtc aaccagttta
      301 ccagcattct atttatgatg ttctacggca tcgttcagga taaactcggt ctgaagaaac
      361 cgctcatctg gtgtatgagt ttcatcctgg tcttgaccgg accgtttatg atttacgttt
      421 atgaaccgtt actgcaaagc aatttttctg taggtctaat tctgggagcg ctcttttttg
      481 gcctggggta tctggcggga tgcggtttgc ttgacagctt caccgaaaaa atggcgcgaa
      541 attttcattt cgaatatgga acagcgcgcg cctggggatc ttttggctat gctattggcg
      601 cgttctttgc cggcatattt tttagtatca gtccccatat caacttctgg ttggtctcgc
      661 tatttggcgc tgtatttatg atgatcaaca tgtgttttaa agataaggat caccagtgcg
      721 tagcggcgga tgcgggaggg gtaaaaaaag aggattttat cgcagttttc aaggatcgaa
      781 acttctgggt tttcgtcata tttattgtgg ggacgtggtc tttctataac atttttgatc
      841 aacaactttt tcctgtcttt tatgcaggtt tattcgaatc acacgatgta ggaacgcgcc
      901 tgtatggtta tctcaactca ttccaggtgg tactcgaagc gctatgcatg gcgattattc
      961 ctttctttgt gaatcgggta gggccaaaaa atgcattact tatcggtgtt gtgattatgg
     1021 cgttgcgtat cctttcctgc gcgctgttcg ttaacccctg gattatttca ttagtgaagc
     1081 tgttacatgc cattgaggtt ccactttgtg tcatatccgt cttcaaatac agcgtggcaa
     1141 attttgataa gcgcctgtcg tcgacgatct ttctgattgg ttttcaaatt gccagttcgc
     1201 ttgggattgt gctgctttca acgccgactg ggatactctt tgaccacgca ggctaccaga
     1261 cagttttctt cgcaatttcg ggtattgtct gcctaatgtt gctatttggc attttcttcc
     1321 tgagtaaaaa acgcgagcaa atagttatgg aaacgcctgt accttcagca atatagacgt
     1381 aaacttttcc ggttgttgtc gatatctccc tatccctcaa ccggaaaata ataatactaa
     1441 agtgcttagc ccggctaata atcacctaat ccaaacgcct cattcatgtt ctggtacagt
     1501 cgctcaaatg tacttcagat gcgcggttcg ctgatttcca ggacattgtc gtcattcagc
     1561 gacctgtccc gtgtatcacg gtcctgcgaa ttcatcaagg aatgcattgc ggagtgaagt
     1621 atcgagtcac gccatatttc gctatcagga ttctgtgtga tggttacatc gcccggccca
     1681 gggctgttta gtcatcagcg ctttctgaca gtgctgagat ttcaacctgt tgcagtaaaa
     1741 atgagtagat ataaggcaag tgtgctgcca aacccatctt ttacggggtg aaggtagatt
     1801 tcgtttgaag ggtatctggt gtcccctgca gacatctact tgaggcggca ggggattgat
     1861 tggaatgctg ttttttagat ttgaaaaata ttttacccgc tattttaccc attggcgcgg
     1921 cttaagagct tatttttaaa ttcacaatgg tcacgatata accatcttgc tcgtccgtgg
     1981 ataactttgg ctttaggcag gtcgccggac ttaatccggt catagatgaa ggtcttaccg
     2041 aagccagtat cagccatgat gaatttcaaa tcaaccagtg aatcaggttg tagttcgtgt
     2101 tgcatgagtg ctatctccga atatggaatc gaacctgcaa atcaggcaat aaaaagccgc
     2161 attgatgcag caatggtagg tctggatatc ttgagaaatg aacaggcctc atcgagtgtg
     2221 aggcgggtta gtccttgcgt agctcgctga ttcttctgta agtctctggt gctttgttcc
     2281 cgtacgtctt catttcagac ttcaacagag caacgagtga atcccattcg ttgaggattc
     2341 ctttgaatgc cggaacgcgc tttgcaacct tgtcgaatga atctctgatt tctggaatct
     2401 gctcaacaag tgcaacgcat cgccgaaagt ctgctgcgtc atggggagcg ccgaagtgat
     2461 gaccatagat attctttttc aggccacatg cgattgaggc aagagttgcg ctactgatgc
     2521 cgacatcgcc agttgattgc catttcaaaa ccttcatagc caaatctgac atttcttgtc
     2581 tccataaaac aaaactcgcc gtagcgagct cagataaaag aaatccccgt cagtgcgagg
     2641 atgctgttca ttgctgctat acactttttt gctctcaacg taagcggtag cccattctgt
     2701 tgggttggtc agttgctttt aggaaatgct ctttacccct taaacgtcgg ctgaaagagc
     2761 taaaatccat gcaaaaaatt tacgcaattt tgtgtattat tgtgcagtaa gtaatgagct
     2821 attttctgtg caaaaaatgg atgataaatt tgtccggggc aggaaaaatt ttatgggcgc
     2881 taaacatgaa aaaagattcg tatccttatt tgatttgcat gacagtttca gggctgatct
     2941 ttattttcct tttcttctgg tggcgggcag atatctacag ggtcacgttt cttaatcaga
     3001 gtatatccca ctattacatt ctgtttagca tgggaatagc ttttctgtta tctctgtttt
     3061 gggttaagaa ggggatagta aaacaaagcg gctggaagag tctgtcagca taccttaagg
     3121 tttatgcagg gatatgcata ttttctggat tttttctgat tatacccctt acaacactaa
     3181 cttatttttt gcctggagag acatcgtctt atgttgcacc gtatcggtat acttccggta
     3241 gttcaaaaag ttgttctgga gctgaggtgg atgaccccga tctacatgag aatattcgca
     3301 tttgctatcc gtatggcaat tatgagtacg ataatattat ctatgttgaa aagaaaatta
     3361 atacattagg tgcggtagtg acatatgcac agaccgcgcg tgatgatact gaatgagata
     3421 gtatatagcg ggcaagtttt agttaattta tcgaggtaat ataatttacc tcgactcgtt
     3481 tattctggta tgaatatttc gctttacgat cgatttttat ctgatgatat catgcggttt
     3541 tcatatactg acttactgtc
//
LOCUS       DAZOZY010000293         3554 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZOZY010000293 DAZOZY010000000
VERSION     DAZOZY010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.296"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(181..441)
                     /gene="yfhL"
                     /locus_tag="ACHURQ_004108"
     CDS             complement(181..441)
                     /gene="yfhL"
                     /locus_tag="ACHURQ_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR0825742.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            656..1846
                     /locus_tag="ACHURQ_004109"
                     /pseudo
     CDS             656..1846
                     /locus_tag="ACHURQ_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(2031..3218)
                     /locus_tag="ACHURQ_004110"
     CDS             complement(2031..3218)
                     /locus_tag="ACHURQ_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR0825743.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(3218..>3554)
                     /locus_tag="ACHURQ_004111"
     CDS             complement(3218..>3554)
                     /locus_tag="ACHURQ_004111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825744.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT          907 a          947 c          827 g          873 t
ORIGIN      
        1 acttaaggtg cgtccggtga accaatcgga cgcgccttta ataactataa ataagtgtct
       61 tggcagatac tatataaatt aacttagtga atgattatgc taatgtcatc aattaaataa
      121 atataatggc gttaaggctt cccagtaata taattaatac tctacttcca gagtagaata
      181 ttaaatttta tccgcgtggt gcatcagcac aaatttatcc cacaactgtt cttctgtctc
      241 gacatgcgcc ggatctttca caatagtatt ggggatcggg cacaccttct ggcaggttgg
      301 tgtctcgtag tgccctacgc attcggtaca cttatcgctg ttaatctcgt agatatgatc
      361 tcccattgaa atcgcctcat tcgggcattc gggttcacac atatcacaat taatacagcg
      421 tttagtaatt agtaaagaca tttcaatgga ttaccgttaa atcattttaa aatcagtaag
      481 ttgtatcgag tttgtatgct ttactgtcat taacttactg tatgttgatc cagtgtattt
      541 aaccttgata aactcagtcc agcaacacaa aaccgcaaca cattgcattt tgtcccgtag
      601 aaaagacttg tatgtgtgag cttgttttct gcgcctacgc agataaggat tgagaatgcc
      661 gcgcactgta acacataatc cggatagccc caataatgac gatgttttag ccgcatctga
      721 aaaatgggac gcctgtaaac ccccctatac cagcgcacac atgaaaatct gtgttgctgc
      781 cgccaaaatc atcctcgctg cttccggcgt ggctcgccgt tccaaatacg aaaaagagaa
      841 ctatctccgt atcgatttca gcaaagccgg taaggttaca ttttacgccg agtttccaaa
      901 aaagatgggc ctcaaaggta aaaagctcgg cgagtggccg gagctcgcta tccagctggc
      961 gcgcgaaaaa gcactaggta tggctgacgg tggactgcgg gcagagtccg tacatgcagc
     1021 gctggaaatg taccgggatg acctcaaagc caaagtagcc cggcagaagc tgagcccgga
     1081 cagtttcaca acctacgggg tgcgtatcga ccggattaaa gcaacgttcg gcgagcgtga
     1141 ggtgttcagc gacgtaacat acaatcggct ggtggaagtg ctggacgagt ggatcgccac
     1201 tcgctcgaac aataacgccc tggagttgtt tgccgagctc cgtcggttct ggaagttctg
     1261 cgcacctact ctttgcaacg gccgcaatgt tgccgccagt ctgccagatg attatgtttc
     1321 ctcccgcgta cagaaaccta cccccacacg gctttttacc gatattgaat caatcgcccg
     1381 actctggctc aatgttgctg cctgcacctc tgtacaccag aagaatgctg ttcgcttcat
     1441 gatcatcact ggtgttcgtc cgattaatgt ccataacctg cgctgggact acgtttacga
     1501 ggaggctggt gaaattgttt atccggaagg ggttatcggc atgcgagggg ctatgaaaac
     1561 acaaaaggct ttccgcctgc cgataacgcc tgagatccgg cggattatcg acgagcagaa
     1621 agcctggcgt gattcagttc ctgagtgcaa cagggattat gtatttttgc agccacgtga
     1681 tccaatgcag ccattttcaa aacgatcact ggataagctg gtgaaaacat acagcccgga
     1741 cggggctgta aaaggaataa aacatgatgg gactgttaaa gggaaagacg gtgcatttaa
     1801 tacgatgtgc cgtaaattcc ttaagagcaa tgttattgcc ttgatcgata ggaattaaaa
     1861 ccccaaaaag attaaaaaaa caccacaaaa cggatgtttc ttcaacacca cttttgctca
     1921 tatgaacgga accgacgatt aaactggatg gctctgattg attcagggta tgaatggcgg
     1981 ttttttgctc cgtttccctc aaaatggacg caactttccc tctgcggctc tcagccgcac
     2041 caccgcatcc gggccagcgg ctcatgcatc aggacctgct ctgccagacg gtagccccgc
     2101 ttcagccccg taaaacgcat ctgactcccg cacagcacgc acttcagcgg gtcaaccttc
     2161 agtaacctct gatacatccc tctccaggtg atttgcatcg ccgtttttct cactgtctcc
     2221 gttatgatgt acaccacttc ttccagtaac cgccgtttcg ccggactcaa aaaaccgtag
     2281 tacctcacca tacggaaccc cttatccgcc acatgccagg agaacctttc catgaactca
     2341 tctccactca tcaacaggta ttcttcccgt tttgttcggt gactgttgta acgcagaccg
     2401 atttcatcct gaccggcata atgctccaga cgactcatcg gcaccggtgg ctttttcagg
     2461 taagagccaa agtacaccgc cacatggggg gcattatcca tcacccggga tacgttgaca
     2521 ttccagccac ggcggtaatg cgtgtccagg aagcgattcc attcccgttt actgcttcct
     2581 tctgctgcca gcgcatccgg catcaccagg tcagggtatt tccgtgacag caaccgtgtt
     2641 atccggtagc gccacatgct catcacctta cgggcgtaaa aatgaagatt tttccaggtg
     2701 tggcccgacg tcacaccacc ggcagttgtc gataaatgga tatgcggatg ccactgctgg
     2761 tcacgccccc atgtgtggat caccgtgaat atccccggct ccacatctgc ctgatggcag
     2821 atttccagta tcacatccgc tgcaatgcgg ctcatctctg tcagtaacca ccggttgtgg
     2881 aacaccaggg accagtactg gcagggaagt gtgaacacaa tatgctgcca cgggcagtcg
     2941 gggaccaggc tcagcagata ctgtatccac tgtgcgccag ccttcacccc gcagtgcggg
     3001 caggagcggc ttttacaccg gaagcagacc ttttttgtat ggcaacagtc cggtgatgaa
     3061 cagcaccact gtgtataccc catcagtgtg gtcccgcacg ccatgatttt ggtcaccgac
     3121 tcaatcacca ccggacgtac tgccctttcc ggctgcttct ccagccagtt aagccagtgg
     3181 tttccctgct gaaagatatc ggcaaaacgg ggaagcatca gaagggcggg gcgactccgt
     3241 ccggccagtg aaccgtgcca cactccgggc agtacatacc gccggcgctg ataccggaaa
     3301 gaatggtcgc aaattcccgc tccgtgcagc gggcgatttc cggataccct tcgtcatcaa
     3361 cacgtacaaa ccagaagacc agctttttgt ttcccgcatc cacaaagaac ggaatattca
     3421 ggtctgcgca gcattcaacg gcatcgtcaa aactatcaaa gcgcagaact tctgcgtctt
     3481 cttcgtcaaa aaaatcatct tcgtgaagct tcacgacata gcggggaagt ttgcttcttt
     3541 gagaggcggg ttta
//
LOCUS       DAZOZY010000294         3543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZOZY010000294 DAZOZY010000000
VERSION     DAZOZY010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.297"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHURQ_004112"
     CDS             complement(<1..140)
                     /locus_tag="ACHURQ_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR0825745.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHURQ_004113"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHURQ_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR0825746.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHURQ_004114"
     CDS             complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHURQ_004114"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR0825747.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHURQ_004115"
     CDS             complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHURQ_004115"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR0825748.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          925 a          982 c          783 g          853 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta
      901 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
      961 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     1021 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     1081 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     1141 cgctcgccgg aaagataagg cagaaaccaa actggctcga cactttcatc agcctgttga
     1201 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     1261 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     1321 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     1381 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     1441 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     1501 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     1561 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     1621 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     1681 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     1741 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     1801 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     1861 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     1921 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     1981 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga
     2041 tgcgggcgcg aaacggtcag cttttccgtt tgcgaatcaa ccacctcacc ctgctcgttg
     2101 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     2161 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     2221 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     2281 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     2341 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     2401 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     2461 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     2521 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     2581 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     2641 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     2701 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     2761 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     2821 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aagccgattt
     2881 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     2941 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     3001 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     3061 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     3121 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     3181 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     3241 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     3301 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     3361 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     3421 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     3481 acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt
     3541 gca
//
LOCUS       DAZOZY010000295         3540 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZOZY010000295 DAZOZY010000000
VERSION     DAZOZY010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.298"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..852)
                     /gene="melB"
                     /locus_tag="ACHURQ_004116"
                     /pseudo
     CDS             complement(1..852)
                     /gene="melB"
                     /locus_tag="ACHURQ_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
     gene            complement(955..2310)
                     /gene="melA"
                     /locus_tag="ACHURQ_004117"
     CDS             complement(955..2310)
                     /gene="melA"
                     /locus_tag="ACHURQ_004117"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR0825749.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2593..3501
                     /gene="melR"
                     /locus_tag="ACHURQ_004118"
     CDS             2593..3501
                     /gene="melR"
                     /locus_tag="ACHURQ_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR0825750.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT          957 a          928 c          831 g          824 t
ORIGIN      
        1 tactaacgtc accaggttag cagctcccgc atacgacaga taatagggga acaaatccgc
       61 atcaccgata acatatgaga aataatagat agcaaatccg gtaataatgt tgctggctac
      121 attataagca agagccatac ccaagaggca tgaaagctga tcgtttttat aaattagcgc
      181 aacgatggct ttaagtgtca gatggcttcc ttcagcagac ggttgattgt ctgacgaaaa
      241 gacttcatgc acattgcgca gagtgatgat ggttgaaaca ataaaaaagg cgatcagtac
      301 cagtgtgaac atctgaaagc caaatccccg atcgccaccg ccgacataat taacaaatgg
      361 tagcgtcaca cctgccgtaa caaagcccgc cagactggca aaaaaacgcg gataaggaac
      421 cagttgttca cgctcacgtt tatcgagcgt gatggttgga accagcgacc agaagggaat
      481 atccataatg gtgtaagtca tgccccagag gatgtaggtc acgcaaacaa agacaatctg
      541 agtagtacct tcaaacagat gcgcactaaa gaggagaaat aagattacag agtttgccaa
      601 cgtaccgatc aggatccagg gtttgaactt acctcatcgc gatcgcgtag catttacaat
      661 ccatcccata atcggatcgt taatagcatc ccagatcctc gccaccagaa acaaagtacc
      721 gaccaaaccc acagacagcc caacgacatc ggtgtagtaa tacatgaggt acatatacac
      781 aatgccgatc gcaaaatcct tcccgaacgc tccaaatcca taactgagtt ttgtagtcat
      841 tgaaatgctc atagggtatc gggtcgctgt taccaacgcc cgccttctgt tattggtatc
      901 gtcagaggtg tgcgccggaa taaatccccc ggcgcagtag cgtttagtcg cgttttaacg
      961 gtgcaaccag cctggcagcc agtcgccgtg ggcggcaatc aggtcgtcaa caagagcata
     1021 tatttcgtca atgcccagca cggcggcagt atgcgggtcc atcatcgcgg cgtggtaaac
     1081 acggtcgcga ttttccgtaa gaatagcttc ggtcagcagc gtctgtacat tgatgttggt
     1141 ttgcatcagg gcggccagat gcgaaggtag cgtaccgact ttagtcggct gaatgccatt
     1201 agcatcaacc agacaggcta cttccacgca acatccttgt ggcaggttat caatcaaacc
     1261 atcgttacgg acgttgccgt aaatcacact cggctcgcca gtccagatag cgttcatgat
     1321 tgtgctggca tattctcgtg acggtttaat atcaatccgg gaggcgtttt tatactcctc
     1381 cagctcttta tgccagttcg ccagctgctc gacgcagcgt ttcgggtact catccagcgg
     1441 tactttataa cgctcaatca aatcctcacg acctggctta ataaaccacg gcgtgtattc
     1501 agcaaaatgt tctgacgatt ccgtaacgaa gtagcccagc tttttgaaca tttcgtagcg
     1561 cacaatattc tggcagcgag tattaccatg aatatttggc ttcggtgcct gccctgcttc
     1621 ataagccgcc agcagttccg ggtagagatt cacgtaactg ccgtcggcgg ttttacgctc
     1681 cagttccagg taaaacgcca tatggttgat accagcgcaa cggtaacgca gcgtggacgg
     1741 atcgatattg aggtcacgcg ccagctcttc cgccgttccc tgcaccgaat ggcacagccc
     1801 tacctgtttg atatgcggat agcgggcata catcgcccag gtattcatcg ccattgggtt
     1861 aacgtagttg agcatggtgg catcggggca gacttccgtc atgtcctcgc aaatttgcca
     1921 cagatgcgga atggtacgta gcgcgcgcat aataccgccc ggccccaacg tatcggcaat
     1981 ggtttgttcc agaccatgcc gcttacagac ctcgaaatca gtcaccgtgc aaggttcata
     2041 accgccaatc tgaaatgcca ccacgacaaa atcggcatcc tgtaaggctt ctttctgttg
     2101 ggtgtggcag gtgattttgc cgctggcccc tgctgaatcc atcagcttac gcaccacaat
     2161 atgcgactct tccaggcggg tgggatcaat gtccatcagg gcaatatgcg ccgttttcag
     2221 cgcctcgcga tggaacacat caccaagaat atttttaacg aaaatcgtcg aaccagcgcc
     2281 gataaatgta attttgggtg cagtcatcat gcagatctcc tggcttgctt gaataacttc
     2341 atcatggcag gcgaatctgc agaaaacctc cggcttccag gttgagcatt cccataaact
     2401 cagatcctgc gtgaagcagc agtaaatctg agtttatggt aataagttac ctgaaaagca
     2461 gagttttcgg cctcatgatg ctcggacgct cccctcgcct ctctgggaag agggtgaaaa
     2521 ccgtgctccc actcgcagtc atcctccctc actcctgcca taattctgat attccaggaa
     2581 agagagccat ccatgaatac agatacgttt atgtgcagca gcgacgaaaa acagacccgc
     2641 agcccgctgt cgctgtattc ggaataccag cggatggaga ttgagtttcg cgcaccgcat
     2701 atcatgccca ccagccactg gcatggtcaa gtcgaagtga atgtgccttt cgatggcgat
     2761 gtggaatacc tgatcaacaa tgaaaaagtg aatatcaatc agggtaatat cacgctgttc
     2821 tgggcctgta caccgcacca actaacagat accggaacct gtcagagcat ggcgattttt
     2881 aatctgccga tgcatctgtt tctctcctgg ccgctggata aagacctgat taaccacgtc
     2941 actcacggca tggtgatcaa atcactggcc acacagcaac ttagcccgtt tgaagtgcgc
     3001 cgctggcagc aggaattaaa cagtccgaac gagcaaattc gccagctcgc cattgatgaa
     3061 attggcctga tgctcaagcg atttagcctc tctggctggg aaccgattct ggtcaataaa
     3121 acctcgcgca cacacaaaaa cagcgtctcg cgccatgcgc aattttatgt tagccagatg
     3181 ctgggcttta ttgccgaaaa ctatgatcag gcgctgacca tcaacgatgt ggctgagcac
     3241 gtcaaactta acgccaacta tgcaatgggg atattccagc gggtcatgca attgacgatg
     3301 aaacagtaca ttaccgcgat gcgcatcaac cacgttcgcg cgttactgag cgataccgat
     3361 aaaagtattc tcgatattgc cctgacggca ggctttcgtt caagtagtcg tttttacagc
     3421 acgttcggca aatatgtcgg catgtcgccg caacaatacc gcaaacttag ccaacagcgc
     3481 cgccagacgt ttcccggcta aagacgatat cagtatcagc caaaaaaata gtttagccgt
//
LOCUS       DAZOZY010000296         3507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZOZY010000296 DAZOZY010000000
VERSION     DAZOZY010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.299"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..2839
                     /locus_tag="ACHURQ_004119"
     CDS             <1..2839
                     /locus_tag="ACHURQ_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR0825751.1"
                     /translation="PGTLNDFLGAMTEDDARPEALRRFELMVEEVARNASAVAQNTAA
                     AKKSASDASTSAREAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEAS
                     KSAAAAESSKSAAATSAGAAKTLETNAAASQQSAATSASTATTKASEAATSARDASAS
                     KEAAKSSETNASSSASSAASSATAAANSAKAAKTSETNARSSETAAGQSASAAAGSKT
                     AAASSASAASTSAGQASASATAAGKSAESAASSASTATTKAGEATEQATAAARSASAA
                     KTSETNAKASETRAESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTAST
                     KATEAAGSATAAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSA
                     TNSTSESLAATPKAVKAAYDLANGKYTAQDATTAQKGIIQLSSATNSTSETLAATPKA
                     VKAANDNAEKRLQKDQNGADIPGKDTFTKNIGACRAFGGSVSTTTGNWTTAQFIEWLD
                     SQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHLAGAVIEVMGIKSAMTIRITTPTTS
                     SGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPTASEIGALPSGGTAVSSVNLASKGR
                     VAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHLKGMTAVGEGELLIGWSGTSGAHAP
                     AFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIPWPSDTVPSGYALMQGQTFDK
                     SAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTK
                     TTSSFDYGTKSTNNTGAHTHSLSGSTSSAGAHQHSQTGPRTNSGSQPTGMFPAGSTQV
                     SGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLSGTAASAGAHAHTVGIGAHTHSVAI
                     GSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            2839..3355
                     /locus_tag="ACHURQ_004120"
                     /pseudo
     CDS             2839..3355
                     /locus_tag="ACHURQ_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          964 a          909 c          973 g          661 t
ORIGIN      
        1 acccggtacg ctgaatgatt ttctcggtgc catgacggag gatgatgccc gtccggaggc
       61 actgcgccgt tttgagctga tggtggaaga ggtggcgcgt aacgcgtccg cggtggcaca
      121 gaacacggca gccgcgaaga aatcagccag cgatgccagc acatcagccc gtgaggcggc
      181 aacccatgcg actgatgctg cagattcagc acgtgcagcc agcacgtcag ccggacaggc
      241 cgcttcgtcg gctcagtcag cgtcttccag cgcaggaacg gcatcaacaa aggccactga
      301 agcctcaaaa agtgctgccg ctgcagagtc ctcaaaaagc gcggcggcta ccagtgccgg
      361 tgcggcgaaa acgttagaaa cgaatgcggc agcgtcacaa caatcagcag ccacttctgc
      421 atccaccgcg accacgaaag cgtcagaagc tgccacctca gcccgggatg cgtcggcttc
      481 aaaagaggcg gcaaaatcat cagaaacgaa cgcatcatca agcgccagta gcgcagcttc
      541 ctcggcgacg gcggctgcaa attctgcgaa ggcggccaaa acgtccgaga cgaacgccag
      601 gtcttctgaa acggcagcgg gacagagtgc ctcagctgcg gcaggctcaa aaacagcggc
      661 tgcgtcgtct gccagcgccg cgtcaacaag tgccgggcag gcctcagcca gtgccaccgc
      721 cgccggaaaa tcggcagaaa gcgccgcatc atccgcttca acagccacaa cgaaggctgg
      781 cgaagccact gagcaggcca cggcagcagc gaggtcagct tccgcagcga agacatccga
      841 aacgaacgcg aaagcgtcgg aaacccgtgc agaatcctca aaaacggctg ccgcatcgtc
      901 cgccagttcg gcggcgtcat cggcatcatc ggcgtctgct tcaaaagatg aggcgaccag
      961 acaggcgtca gcagcgaagg gcagcgccac gacggcatcc acgaaggcga cagaggctgc
     1021 tggtagtgcg acggcagcag ctcagagcaa aagtacggcg gaatctgcag caacgcgcgc
     1081 tgagacagcg gcaaaacggg cagaggatat tgcatccgcc gtggcgcttg aggatgcgag
     1141 cacgacgaaa aaggggatag tacagctcag tagtgcgact aacagcactt ccgagtcact
     1201 ggcggcaaca ccaaaagccg ttaaggccgc gtatgacctg gctaacggga aatacaccgc
     1261 acaggatgca acgacagcac agaaagggat aatccagcta agcagcgcga ccaacagcac
     1321 gtctgaaacg ctggcggcaa cgccaaaggc agtaaaagca gccaatgaca atgctgagaa
     1381 acgtctgcag aaagatcaga acggtgcgga tatccctggc aaagacacct ttacgaaaaa
     1441 tattggtgcc tgccgtgcct tcggtgggtc agtaagcaca acaacaggaa actggacgac
     1501 tgcacagttt atcgagtggc tggattctca gggagcattt aaccatccat actggatgtg
     1561 caagggttcc tggtcttatg gcaataataa aatcattact gatactggct gcggtaatat
     1621 tcatctcgcc ggagctgtca ttgaagtaat ggggataaag tcagcgatga cgatccgcat
     1681 taccactccg accacatcca gcggtggcgg aacaaccaac gcccagttta cctatattaa
     1741 tcacggaaca gattattcac ctggctggcg aagggactat aactccagaa ataagccaac
     1801 ggcatcagag atcggggcgc taccgtcagg tggaacagca gtatcatcag ttaatctggc
     1861 ttcaaaaggt cgggtagccg cgctgacaga caatacgcag ggggcagcag gtcttgagtt
     1921 atacgaggtg tataacaacg gatatccaac agcgtatgga aatatcattc acctgaaagg
     1981 gatgacagcc gttggcgaag gtgagttact catcggctgg agtggtacaa gcggtgctca
     2041 tgctccggca tttattcgtt cacgacggga tacgaccgac gcaaactggt cgccgtgggc
     2101 gcagctttac acctcggctc atcctcctgc agagttttat ccagtcggtg caccaatccc
     2161 gtggccatca gataccgttc cgtctggtta tgccctgatg caggggcaga cttttgacaa
     2221 atctgcttac ccgaaacttg cagccgctta tccgtcaggc gtgatccctg atatgcgtgg
     2281 ctggacgatt aagggcaagc ccgccagtgg tcgtgccgta ttgtctcagg aacaggatgg
     2341 cattaaatcg cacacccaca gcgccagcgc atccagtacg gatttgggga cgaaaaccac
     2401 atcgtcgttt gattacggca ctaaatccac gaataacacc ggggcgcata cgcacagtct
     2461 gagcggctct acaagttcag ctggggccca ccaacatagt cagactgggc ccagaacgaa
     2521 tagcgggagt caacctactg gaatgtttcc ggcagggagt actcaagtta gcgggactaa
     2581 tcaggttggt atcagtggct cattaaccag tggcacctcc caatgggtag gtaaatcatc
     2641 ttcagaagga aaccatacac actcactgtc cggcacagca gcatctgcgg gtgcacacgc
     2701 gcatactgtc ggtattggtg ctcatacgca ctctgttgcg attggttcac atggacacac
     2761 catcaccgtt aacgctgctg gtaacgcgga aaacaccgtc aaaaacatcg catttaacta
     2821 tattgtgagg ctcgcataat ggcattcaga atgagtgaac aagcacggac cataaaaatt
     2881 tataacctgc tggccggaac taatgaattt attggtgaag gtgacgcata tattccgcct
     2941 catacaggtc tgcctgcaaa catcgtggca tctcgttgaa gatcatcggg gtaaaacggt
     3001 ttatgacgtg gcttccggcg acgcgttatt tatttctgaa ctcggctcat taccggaaaa
     3061 tgtcacctgg ttatccccgg aaggggagtt tcagaagtgg aacggcacag cctgggtgaa
     3121 ggatacggaa gcagaaaaac tgttccggat ccgggaggcg gaagaaacaa aaaacagcct
     3181 gatgcaggta gccagtgagc atatagcgcc gcttcaggat gctgtggatc tggagatcgc
     3241 aacggagaaa gaaaccttgt tgctggaagc ctggaaaaag tatcgggtgt tgctgaaccg
     3301 tgttgataca tcaactgcac cggatattga atggccagta gcacctatag ggtaaattcg
     3361 taatgattac ctaaatacgt tattcttttg ttaaaaaagt gattttgcta taggtaggga
     3421 gtatgttctt ggattaagaa taaaaaatcg ccatgaaaga gcttcatggc gagtacataa
     3481 acgtacaaaa tgtgatgaaa gtagagt
//
LOCUS       DAZOZY010000297         3448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZOZY010000297 DAZOZY010000000
VERSION     DAZOZY010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.300"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1361)
                     /locus_tag="ACHURQ_004121"
     CDS             complement(<1..1361)
                     /locus_tag="ACHURQ_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR0825752.1"
                     /translation="MNRTSPHYCRRSVLSLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1426..1545)
                     /locus_tag="ACHURQ_004122"
     CDS             complement(1426..1545)
                     /locus_tag="ACHURQ_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR0825753.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1769..1927)
                     /locus_tag="ACHURQ_004123"
                     /pseudo
     CDS             complement(1769..1927)
                     /locus_tag="ACHURQ_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2272..>3448)
                     /locus_tag="ACHURQ_004124"
     CDS             complement(2272..>3448)
                     /locus_tag="ACHURQ_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR0825754.1"
                     /translation="NEELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSN
                     KNNVDQFIECAKRNEQTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDN
                     QIDKVSPVTKNSQQVKGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITD
                     AFVKEFRGVLIDKNGNCSERLFNFYECCYKFLPRAQPQDKIESYNSALQAFSIFRSST
                     LNNNDVGFNFKLFPEVKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGL
                     DFKGCFFSGQNFSNYDIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSG
                     VLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT          950 a          784 c          621 g         1093 t
ORIGIN      
        1 actaaagcac tgcccgtctc ctggttaacc ccatgtgcta taccgccggt atagacaatc
       61 agcgagcctc cggcgctaat attgctgcca gttgccgtac catctttctc aacaacctgc
      121 cggctcccgg tggaaatatt tgtcgtgtca gctttcccgc cgctcttgat attttgtgtt
      181 ccgctgttga tattggtacc cgtggcaata ccatgattat taatattctg tgtaccacca
      241 ttgattatgg tattggtcgc gtttctggta acatccatta ccccgccgtt atctattcgg
      301 gtcgcatcag ctttagcatt cgttaaaact gacattgttc ctttatcttt aataatcgtc
      361 ttatttgccg aaccatatgc gtttatgtct aaatgaccac cgttttccag caacacattg
      421 tctgccacgt gattgtggat ggagaatgca ccttcactat tcgtaccgct caccgtcgta
      481 ccgttagtgt ttgtttttaa aattgcacca tcgtgctggg taacatttgt tgccgtacca
      541 ccactaacat caagcacgcc accggaataa acttcaataa catccgagga gctggtgtaa
      601 tcaacaattt gcgtaccacc agaatagatc tgagtatttt ttgccgttga cttattattc
      661 agagactgag ttccaccttc aatcgtcgtg tccagcgcat ggctttcata tactctttgc
      721 tcaccgccat ttttaatggt ggttgttcct actgtgctct gttcaacata ctgtcgtcca
      781 ccatttatga ttgtgttcgt tgccatactt ccttgcgtga tgtcctgata accggactta
      841 tttatcgttg taccatcaac atgcccctga gttgttactg tctgccctcc accatcaatg
      901 atggttccat tcgcagtccc cccacttatg tgtgaattac cacccttaat tgtcgttcca
      961 ttgctgatac cacctgtata aacgtcctga ttgccactgt cgattatcgt accggtggaa
     1021 atgcccccgt catgaattgt ctgtcttcct ccgttaatgg ttgtattatt agactgcccc
     1081 atataattgt tattccttcc aacatcttga tatccaccag attcaataag acttccatta
     1141 gatactccgc cataaacact ctgctggcca tggttgataa tatgagtgtt atttgttgta
     1201 cctcgttcat ctactcgttg gttgccatct acagtctcat cgtttaccac accaataaca
     1261 ttagtagtaa aggccgccat cacgggcggg gcatatatca aggcagatat caataaggaa
     1321 agtactgagc ggcgacaata atggggactg gtcctgttca taaatttcat cctctgaaaa
     1381 gtgaatactg agtagcgttt aagcgacttt agctctgctg caacatcagc ccgcaggcac
     1441 cagaccgggg ggttcatcct gaagagacag cgcaagtgta ttgtgttcac cgctcatcaa
     1501 agacatcatg atgaaatgat gatgttccac ataagaaaga ggcatttttt aaacacagtg
     1561 cgctgaagtg tgattggata aaaaagtcaa tctattcagg aaatacgggc gtattctttt
     1621 ctttcgacaa tcaggccgtc ggcaaaataa aatgatttac ataatcgttt ctgatgaata
     1681 tcttctgctc acataaaaat cacacaataa ctttgagatc gcagattctt ttacttttac
     1741 agcattcgtc cccccattgt tgggcaaata tagattgggc cagagcacga aagttaatac
     1801 cacgtttgca cagctcctcc aacagcacga caagatgcca catactgcgc cccagtcggt
     1861 gcggtttaca gactacccgt gcgtccaccg ccgataatgt cctgagcagt tttttcagtc
     1921 cggacctcct gccacggttt tacgttgaag aggtaaccct gagcacgcag ttcttcagtc
     1981 aggcgtggtg caccgtaacg ctgttattgc tgggtaagat caaaaaactt tcaggcagct
     2041 aaggaaagtt gaaccagaca ttagatgaaa tatttcaacc aattacagca ccaatttaaa
     2101 ctcagtaaat cacagcacag atcaaggggt tacgtgaaag cgtagcccct ttttctttgg
     2161 tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca
     2221 tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa
     2281 actgctttta gaacttgata caacgggact agtcacaaca ggactattct caacgggatc
     2341 atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc
     2401 cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt
     2461 ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat
     2521 acacggagta tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt
     2581 actgaagttc tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa
     2641 accatcctct tctttttgga gagtaatgtt aattctggct atctcccgga caaaacttcc
     2701 ttttttgtat ttgaataccg tttcaagatt ttcaccagac agcttgactt ctgggaataa
     2761 tttaaaatta aaccctacat cattattatt caaagtagat gaacgaaaga tggaaaaagc
     2821 ttgtagtgct gaattatagc tttcgatttt atcttgaggc tgcgctcttg gtaaaaactt
     2881 atagcagcac tcataaaaat tgaataaacg ttctgaacaa ttcccatttt tatcaattaa
     2941 taccccccgg aattctttca caaaggcatc cgttattttt tgaatatcta tggtgtgttc
     3001 ctttgactct gacaaagatg gagtctgttg aagtgcattg ctcatctggt taaggaacat
     3061 ctgcatttct ttaaatttat ctccatagtg gcctttaact tgctgagagt ttttagttac
     3121 aggtgaaacc ttatctattt ggttgtcata taaattatgt ggttccatac agacatttgg
     3181 ttgtgtttct aaaataacac tacgtgtact acctggctta aatgaatcaa catgaaaatc
     3241 actttttctt atattatcga atagggtctg ttcatttctc ttagcgcatt caataaactg
     3301 atcaacatta tttttattcg atgacaggtc agtttcagaa accgcatttt ctttgttctc
     3361 cagctttagt ttaatgagaa gatcttccca gacctgcttg cttaaacata ctacgtcagg
     3421 ctcaccatca gtaactaact cttcatta
//
LOCUS       DAZOZY010000298         3443 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZOZY010000298 DAZOZY010000000
VERSION     DAZOZY010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.301"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..302
                     /locus_tag="ACHURQ_004125"
     CDS             <1..302
                     /locus_tag="ACHURQ_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825755.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            391..2259
                     /locus_tag="ACHURQ_004126"
     CDS             391..2259
                     /locus_tag="ACHURQ_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR0825756.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2296..>3443
                     /locus_tag="ACHURQ_004127"
     CDS             2296..>3443
                     /locus_tag="ACHURQ_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR0825757.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAKRSERTDEIISTLEYIDN
                     TLITFSNEEIIKCQIAIQSIINKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRT
                     FATALANIEKYPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECD
                     LNLVSGESLIRQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILK"
BASE COUNT         1088 a          625 c          668 g         1062 t
ORIGIN      
        1 cacgtgtaga actgtattac cgctttaacc atgaagaaga atattaccct tctcaccgtt
       61 ttatgcttgt tgttgatatg aagacagtaa tggttttttc acaacgagct gaatcactgg
      121 ggttatactc gaataatcgc ttgcttatca atataattgc cgaacatatc catagtgata
      181 tatatctaac caagtatcag caacaatatc cagatgccaa tgcattaata aatactttgc
      241 atgaatctgg caatgatatg gtgcaggatg aaaaacattt gagtgatagc aaaaaccgtt
      301 aatattttcc tttcattgtg atcgtctgta ggcgatcaca atctatttgt cttttataac
      361 gtctaaactt ttttatgcaa aggtgtattt atgatttttt ttaacgatat aaagcccact
      421 ggttggttaa agaataagat ggaatggtat atgaacggtc atataggaga actggataaa
      481 ttagttcccc atcttattac ggagcataaa atatatggaa gagatcgtat aactccacgc
      541 actgtatctg ctgaagatgg cgttattaaa aaacagaata atcccgatga taacatgatg
      601 caatattatt ggtgggatag tgaaactcaa tcgaattgga aggatggata ttgccgcagt
      661 gcctacttgt tagataataa agaatggcag gaaaaagcga gcgatttatg tttggaactt
      721 atcgatacgc aagatgatga tggctacatt ggaatttatg attcatcatt aaaatttaac
      781 tgtaaagcag aaaacggaga gttctgggca caagctactg ccttgcgttt tgtgcttgga
      841 tgttatgagt ccacccgtaa tgagagttta ttaacttctc ttacccaagc cgcacaatgc
      901 attatggctg gctaccctaa agaaacgtca catcctttcg ttgtggaaca gggttttgct
      961 ggacatagcc acggattaac aataacagat gttttctacc gcctttatga aataaccaat
     1021 aacattgaat atctctcata ttgcttgtgg ctgtatgaag attattccgc aggatgcgtg
     1081 tcggagcaag atctgcaata taaaaatgtc atagaccctg gttaccttct taaaggtcat
     1141 ggcgtacata cgtatgagca tattagggca ttagccatcg cagcgagcca acggccgcag
     1201 tatcaaactg cattagatat ttttttatcc cagttgaaat attatcttac accatctggc
     1261 gctcccattg gtgatgagtg gattacagga cgcacagcta atgccactta tacaggctat
     1321 gaatattgtt ctgttcatga actatttagc acttatgtta tgttaataaa actgaccact
     1381 aatattggtc tggccgatga tgctgagtgg ctattctata atgctagttt tggcatgcag
     1441 catcccatac ttcacgcgat aatgtactgt aagactgata attgttatga agcaaatgaa
     1501 aggaaacatc ctgacagtaa tcagtttaat accagatata aatactcccc tactcatgag
     1561 gaggctgctg tctgctgcgt accgaataca gcaagaacca taccggcttt tgtcgaaaat
     1621 atgtttcaag aaaataacaa tggttttact gcattatttt atggcccgtg tgagttttat
     1681 ggcacattta ataatgttgc aattaaaatt acacaattaa ctgaataccc tcatgattta
     1741 agcgtaaaat gtctcattga gcctgagtca tctgttcgtt ttgctctcag tttccgttac
     1801 cccggctggg caaaaatgat gattattaat ggtgttactt ttactaaaaa tgatactcaa
     1861 aactcactta ttattttaga tagaacatgg caacatcatg atgtgattga cattaaattt
     1921 atagctgata tacagtttaa tactgactta tgtggtgaca cttatatttc cagaggtccc
     1981 gttttatatg caattgagct tgagagcgat attttaatca aaaaaaacct gctgaacggg
     2041 aaatattttg actcaggata cataccctgt agtagagctg atgaatctat ccagttctct
     2101 tatgctgata tggaatcttt ttcatattca acctcaccag agggcaaaca atacattgaa
     2161 ggctgttttt atttaaacaa gactaaaata gtcagaaaac ttataccatt tggtcaaacc
     2221 attcttcgca aagtaacttt ctccaatata gagagttaat attttcgagt gttataatta
     2281 attaaaggga tttctatgta ccttactgat aatattatca aactgtcgaa taaaataggt
     2341 cagcgtgttt atactgttgt ctctgaaatg tcgattgaag catggataac aaaagaaccg
     2401 cagccttttg tttatcgccg tagcggcgct taccagaaac tcaccattgg ctcagactgg
     2461 ggtcaactgt ttgactgtgc ctggatgcga gtttatggta aacgtccagc agataaattt
     2521 agacacaaac tcgtcgctct cgttgatatc ggtggcgaag gtcttattgt ggataagaat
     2581 ggttcaccca tctgcggcat cacgaataaa gcatcatctt atggcgtacc gccagataaa
     2641 ccaggtaaat gggtggttga cctttctctc gttagtgaaa atgacgaggt tgagttttgg
     2701 attgatgctg cctgcaacga tttatttggc tatgttacta atggagggat tattactgat
     2761 gttcatatcg cgacacgtaa tcaactactg aaatcccttt attatgatat tgaggtgctt
     2821 ttcgactgga ttaatgatgg tcagaagttt gaatccatac atcctaaagg tatcacccca
     2881 gaacaattta ttgccaaaag aagtgagcgt actgacgaaa tcataagtac tctcgaatat
     2941 attgataata cgttaattac ctttagcaat gaagaaataa ttaaatgtca aattgcaatc
     3001 caatccataa tcaataaaaa caataatcct tcagagttca ggattatggc tactggtcat
     3061 gcccatttag atatagcgtg gatgtggcct ttaagagaag gacgcagaaa agcaataaga
     3121 acatttgcca ccgcccttgc aaacattgaa aaatatcctg attacatttt tggcgctagt
     3181 cagtaccaac tttttcactg gataaaaaaa gattatccct atttcttcga aaagttaaaa
     3241 aaacaaatcg ccgcaggccg ctttgagtta caaggttgct tctgggtcga atgcgattta
     3301 aatctggtaa gtggtgaatc gctgataagg caaatcatgc atgggacacg attcacactg
     3361 cagaattttt caaaaaagat aaattatgtc tggcaaccgg atgtctttgg attccccgca
     3421 acgcttccac aaattctcaa aaa
//
LOCUS       DAZOZY010000299         3421 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZOZY010000299 DAZOZY010000000
VERSION     DAZOZY010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.302"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            764..1450
                     /locus_tag="ACHURQ_004128"
                     /pseudo
     CDS             764..1450
                     /locus_tag="ACHURQ_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1783..2658
                     /locus_tag="ACHURQ_004129"
     CDS             1783..2658
                     /locus_tag="ACHURQ_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR0825758.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2666..>3421
                     /locus_tag="ACHURQ_004130"
     CDS             2666..>3421
                     /locus_tag="ACHURQ_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR0825759.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPI"
BASE COUNT          939 a          802 c          808 g          872 t
ORIGIN      
        1 gccagactaa ttattaataa tccagttatc tttttattca tatcatctcc tttccattgg
       61 ttaatttatc ggaaattcat ccccactaac taaaacgatc cacacaataa aactaaatct
      121 aattgcaggc aatcaattcc agtcgatata tcatctgtat ggaaatcatc gctcatcact
      181 catcgcagcc tgaaagccca gagcaacggg gcaggtctgc atataagtgg ttacacggca
      241 gaattccaga gggttctgtc actaaattca gtcactgaca aatttgcatg atgttgaata
      301 gacgagattt tgtgtcgaaa tagttgtttc agtaactgtc actcaatcgg tggtttatgt
      361 gactatattc gcaaaaactg gtcgcagaaa agtcctgttt tcagcttgga aacccggtaa
      421 tcagaataat ggatagggga acaaaaatct ctgtatagaa cgctccattg agggagagaa
      481 aacgtgtcat tttgacccgt tcggtattca gaacacacaa aaccgatcgc gggtcgcttt
      541 cgtgcatagt tatgcaacgc gctaaaaacg attctgacgc attttttatg tttaccctgt
      601 tttttcttgt ctttttgagt tttttgcgtc agagagcgtc tgagagggca cgtacaacgg
      661 gagttatggt aaatggatcg ggttttcggg aaggttcgac aggatttgcc gttgggtgta
      721 gtgtaagcga ctgaaaaaca aacgcccctg aaatcatgtc cagatccggc agaaccagcc
      781 tcatgcgcag ttctggctgg tttttgacgt tgatcgcgag ggggcagcga ttgactggag
      841 cgaccggaac gctcccgcgc cgaacatcac cgtaaaaaat cctgtgaacg gtcatgctca
      901 cctgctctac gcgctcaata tcgccgtgag aaccgcgccg gacagctcgg ttaaggcgct
      961 gaaatacgcc gctgcgattg aacgttcgct gtgtgagaaa ctttgtgctg atgtgaacta
     1021 cagcggactg atttgtaaaa atccgttcca cctggaatgg caggtgatgg agtggcgaga
     1081 ggaagcctat accctcgatg aactggctga ttatctcgat ttgagcgcct cagcgcgccg
     1141 tagcatcgat aaacattacg ggatggggcg aaactgccac ctgttcgaaa tgacgcgcaa
     1201 atgggcttac agggcgattc gtcagggctg gcccgaattc tcacagtggc ttgatgccgt
     1261 gattcagcgt gtcgaaatgt acaacgcatc gcttcccgtt ccgctttcac cgcctgaatg
     1321 tcgggctatt ggcaagagca ttgcgaaata cacgcacagg aacttcacgc cggaaacttt
     1381 cgcacagtat gtggctgata cgcacacatt aacgtacgtt ttcgttccat tggccctcac
     1441 cccccgataa cgacccaggt agcgcgccgt attccccccg ccggccgttt tcttcgacat
     1501 gtacatcagg cgcggaactc atcggggcgc gggaatgaaa tcccattcag actccggata
     1561 gattcaagca agcccactta tcgtcaaaat cggtgttgca aaaacgggag tgaccataga
     1621 tccgttttcc aagcggaccc cataatatcg gctggtaaac atcactcgct agtgcattct
     1681 ttttgttggt aatgataatc atttacattc attctttgat gttttatcat aattaatgtt
     1741 ttgtcaataa atatatggaa acatattgtt tcagatggga atatgagaaa gttcatcctt
     1801 atttctatga taacgttagc tagtgttagt ctgggagcct gcagacagaa tgtcaccata
     1861 aagcaggatg cgccagggca gaaagctttc ctgactgacg ggcagattta cgatttgcac
     1921 agtggcaaaa ttatttctag cagcgagctt ttggcggatc ttgctaccgc tcgacatctt
     1981 attattggtg aaaaacacga taatgccgag catcatcaga ttgagctttg gcttattcaa
     2041 aatttgctca tccagcgacc acaggggagt gtgttgcttg agatgttgac gtcagagcaa
     2101 cagccccgag ttaaccaagt taaatgttgg ctgaaagata atcctgtggt tcgagatagc
     2161 cgtgttcagg aactacttaa ctggcaaaaa gggtggtcct gggagatgta tggcgacatc
     2221 gtcatgcagc tattacgtgg gccttacccg ctgctcaatg ccaacattgg ccgggagcag
     2281 atgttggcac tgtacaaaaa aaacgaattt cctaaaggaa aaaaatccac tgcacctgtg
     2341 gtacaagaag ctctgcgtga aaccatcata tctatgcatg aaggcaacct tgaaagccag
     2401 caactgacgt caatgctctc tattcagcag cagcgggatc gctacatggc ccgccagctt
     2461 ctgagtgccc cggtaccgtc gttgttgatt gcaggtggtt atcacgcgtc aaaaagcatg
     2521 ggcgtaccgt tacacatgga agatcttgcc accggcaccc atccagtggt gttgatgctt
     2581 gcagaaaaag gaatgaacat cacagtagat catgctgact acgtgtggtt tgtagcgcca
     2641 gatacaacaa aaaggtaaac catggatgat gaatattctc cacttctcac agtcggtaaa
     2701 atggagcagc tggtttatct gttctctact gcttcatgga ctgattttcc ttgcgtttat
     2761 ctggcgtttt tccgaagttc agcccgctat gtctcctgca ccggcgatca tgctgcaatg
     2821 ggcagaaatt atagaggctc cttcttctcc actttcgctt ccggtgggaa tcgctcaaca
     2881 ggagtccgca gtgacagaag aaaagcaaca aactgaggac agacaacaac gtcctgtcac
     2941 tgaagacagt gatgcaacca ttgaaataac taggaagaaa aagtcatccg atggagaaaa
     3001 gaaaaagaca cgtccacctc gtaagataaa ggctcaaacc agcgacagca accccacagc
     3061 cgtttccagt aatgctgcac ctcaggctct ggtcgaatcg tcccgtattg ctgcgccttt
     3121 taacagtgac tcaacaaagc gagataattc tgaggcatct tgggaaagca gagtaaaagg
     3181 ccacctaaac cgttataaac gttaccctgg cgatgccaga aaacgtgcac gaaccggcac
     3241 agccgtcgtg acttttaccg taaacacgga aggcactatt gtcagtagtt tcttggaaat
     3301 ctcttcagga accctttctc ttgaccgcga agctattgct gttttagaac gcgctcaacc
     3361 acttcccaaa ccaccgcctg aaatattgga gggaggatta tttaaagtaa aaatgccgat
     3421 t
//
LOCUS       DAZOZY010000300         3365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZOZY010000300 DAZOZY010000000
VERSION     DAZOZY010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.303"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHURQ_004131"
     CDS             complement(<1..312)
                     /locus_tag="ACHURQ_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR0825760.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHURQ_004132"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHURQ_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR0825761.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHURQ_004133"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHURQ_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR0825762.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          914 a          874 c          733 g          844 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgagggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatg
//
LOCUS       DAZOZY010000301         3322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZOZY010000301 DAZOZY010000000
VERSION     DAZOZY010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.304"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHURQ_004134"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHURQ_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR0825763.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHURQ_004135"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHURQ_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR0825764.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENQLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="ACHURQ_004136"
     CDS             complement(1250..2824)
                     /locus_tag="ACHURQ_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR0825765.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="ACHURQ_004137"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="ACHURQ_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          852 a          789 c          712 g          969 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagct ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAZOZY010000302         3307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZOZY010000302 DAZOZY010000000
VERSION     DAZOZY010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.305"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..2976
                     /gene="sigA"
                     /locus_tag="ACHURQ_004138"
     CDS             <1..2976
                     /gene="sigA"
                     /locus_tag="ACHURQ_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR0825766.1"
                     /translation="SLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVSGGGE
                     LTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGDNLHK
                     IGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSESGNGE
                     IYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGNVSGN
                     ISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGNNNCP
                     IPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNLENAT
                     LSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSAGESS
                     FNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIELAGT
                     LTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILFGVDQ
                     YKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQSLKTR
                     DSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGSNNSL
                     SVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEHTTWT
                     LTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARIMSGA
                     GSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSVGAGL
                     YASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHVTEDS
                     WIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTA
                     RAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLEFEKS
                     AFGKYNVDNAINANFRYSF"
     gene            3035..>3307
                     /locus_tag="ACHURQ_004139"
     CDS             3035..>3307
                     /locus_tag="ACHURQ_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825767.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIV"
BASE COUNT         1072 a          667 c          735 g          833 t
ORIGIN      
        1 tcgttattta ataatattct ggtaaacaat ttaaaaaaga atttcacaaa cactatgcag
       61 ctggatggta aaaaacaaga gttatcatcg attataaaag ataaggacct gtctgtctca
      121 ggaggagggg aattaacgct caagcaggat accgatcttg gcattggcgg gcttatattc
      181 gataagaacc agacatataa agtgtacgga aaagataagt cttataaagg tgccgggata
      241 gatattgata ataataccac cgttgaatgg aatgttaagg gcgttgccgg agataatctg
      301 cataaaatag gtagtggtac tctggatgta aaaatagcac agggaaataa ccttaaaata
      361 ggtaatggga ctgtcatcct tagtgctgaa aaagccttca ataaaattta catggccgga
      421 ggtaaaggta cggtaaaaat aaatgccaaa gacgctttaa gcgaaagcgg taatggcgaa
      481 atctatttta ccagaaatgg cggaacactg gatctaaacg gctatgacca gtcatttcag
      541 aaaatcgcag caacagatgc gggaacaacc gtaacgaact caaacgtgaa gcaatcaaca
      601 ttatcactta ctaatactga tgcatatatg taccatggga atgtatcagg taatataagc
      661 ataaatcata ttatcaatac tacccagcaa cataacaata atgccaatct gatctttgat
      721 ggctcagtcg atatcaaaaa cgatatctct gtccggaatg cacagttaac attacaagga
      781 catgcgacag aacatgccat atttaaagaa ggcaataaca actgtccaat tcctttttta
      841 tgtcaaaaag actattctgc tgccataaag gaccaggaaa gcactgtaaa taaacgttac
      901 aatacggaat ataagtccaa caatcagata gcctcttttt cccagcccga ctgggaaagt
      961 cgtaaattta atttccggaa attaaattta gaaaacgcaa ccctgagtat aggccgggat
     1021 gctaatgtaa aaggacacat agaggctaaa aactctcaaa ttgttctggg aaataaaact
     1081 gcatacattg acatgttctc aggaagaaac attactggcg aaggttttgg attcagacaa
     1141 cagcttcgct ccggggattc agcaggcgaa agtagtttca acggcagtct gagtgctcaa
     1201 aacagcaaaa taactgttgg tgataaatca actgttacta tgactggtgc attatcctta
     1261 attaatacag acctgattat caacaaagga gctactgtta ccgcccaggg aaaaatgtat
     1321 gtagataaag ctattgaact ggccggaacc ctgacattaa caggcacccc tacagaaaat
     1381 aataaataca gcccggcaat ctatatgtca gatggatata atatgacaga agatggtgcc
     1441 acgttaaagg ctcaaaatta tgcctgggtc aatggtaata taaaatcaga caaaaaagca
     1501 tctattctgt ttggtgttga ccagtataaa gaagataacc tggacaaaac cacacacaca
     1561 ccgctggcta caggtttgct gggtggcttt gatacttctt ataccggagg tattgatgct
     1621 cctgctgcct cagccagcat gtataacacc ttatggagag taaacggaca gtcagccctg
     1681 caatcattaa aaacccgcga cagtcttttg ttgtttagta acatagagaa ttcgggtttc
     1741 catactgtga cagtaaacac actggatgcc actaatactg ctgtgattat gcgggctgat
     1801 ctgagccagt ctgtaaatca atcggataaa ctcattgtta aaaatcagtt aaccggaagc
     1861 aataacagtc tgtcggtcga tatacagaaa gtgggaaata ataactcagg attaaacgtt
     1921 gacctgataa cagccccaaa aggaagcaat aaagagatat ttaaagccag tactcaggcc
     1981 ataggtttca gcaacatatc tcctgtgatc agcacgaaag aggatcagga acataccacg
     2041 tggaccctga ccggatataa ggtggctgaa aatacagcat cttccggtgc agcaaaatcg
     2101 tatatgtccg gtaattacaa agccttcctg acagaagtca acaacctgaa taaacgaatg
     2161 ggggatctgc gtgacaccaa tggcgaggcc ggtgcatggg cccgcatcat gagcggagca
     2221 ggttcagctt ctagtggata cagtgacaac tacacccatg tgcagattgg tgtggataaa
     2281 aaacatgagc tggatggact tgaccttttc actggtctga ctatgacgta taccgacagt
     2341 catgccagca gtaatgcatt cagtggcaag acgaagtccg tcggggcagg tctgtatgct
     2401 tccgctatat ttgactctgg tgcctatatc gacctgatta gtaagtatgt tcaccatgat
     2461 aatgagtact cggcgacctt tgctggactc ggaacaaaag actacagttc tcattccttg
     2521 tatgtgggtg ctgaagcagg ctaccgctat catgtaacag aagactcctg gattgagccg
     2581 caggcagaac tggtttatgg ggccgtatca ggtaaacggt tcgactggca ggatcgcgga
     2641 atgagcgtga ccatgaagga taaggacttt aatccgctga ttgggcgtac cggtgttgat
     2701 gtgggtaaat ccttctccgg taaggactgg aaagtcacag cccgcgccgg ccttggctac
     2761 cagtttgacc tgtttgccaa cggtgaaacc gtactgcgtg atgcgtccgg tgagaaacgt
     2821 atcaaaggtg aaaaagacgg tcgtattctc atgaatgttg gtctcaacgc cgaaattcgc
     2881 gataatcttc gcttcggtct tgagtttgag aaatcggcat ttggtaaata caacgtggat
     2941 aacgcgatca acgccaactt ccgttactct ttctgataac agcccgggcc gcgtttgcgg
     3001 cccttcttct accgtagaga atatgtatta ccctgtgaca gactatatcg ctcttgctct
     3061 cattattagc tttctttttc tgacattatt tatctgcctg ttatgcctta aacatgagcg
     3121 aataaaaaaa gaaactatca ggcaaaaaaa tgcacatatt ctggagcatg gctggaatgc
     3181 aactgagttc tcatggttcc gatacggaca gtataacgaa acgggtattt acatctcgat
     3241 cgagaagacc attatcatta caatcaccgt ttctggtgag tgctttaaaa aagaatacag
     3301 cattgtc
//
LOCUS       DAZOZY010000303         3218 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZOZY010000303 DAZOZY010000000
VERSION     DAZOZY010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.306"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            101..817
                     /gene="nanC"
                     /locus_tag="ACHURQ_004140"
     CDS             101..817
                     /gene="nanC"
                     /locus_tag="ACHURQ_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR0825768.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            837..1943
                     /gene="nanM"
                     /locus_tag="ACHURQ_004141"
     CDS             837..1943
                     /gene="nanM"
                     /locus_tag="ACHURQ_004141"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR0825769.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2008..2988
                     /locus_tag="ACHURQ_004142"
     CDS             2008..2988
                     /locus_tag="ACHURQ_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR0825770.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3098..3218
                     /locus_tag="ACHURQ_004143"
                     /pseudo
     CDS             3098..3218
                     /locus_tag="ACHURQ_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT          949 a          682 c          759 g          828 t
ORIGIN      
        1 aagacactat aagcctggca aaaaatatta cacaacataa atgctaattg tttatgcggg
       61 ctttgtattg ctttctgtat cctacaaatg agtgaaattt atgaaaaagg ctaaaatact
      121 ttctggcgta ttattactgt gcttttcgtc accattaatt tctcaggctg cgacactgga
      181 cgtacgtggt ggatatcgta gcggaagcca tgcctatgag actcgactca aagtcagtga
      241 gggatggcaa aatggatggt gggcaagcat ggaaagtaat acctggaata ccattcatga
      301 taataaaaag gaaaatgccg cactcaatga tgttcaggtt gaagttaatt acgcgattaa
      361 acttgatgat caatggacgg tgcgcccggg aatgttaacg cattttagca gcaacggcac
      421 acgctacgga ccctacgtaa aactgtcctg ggacgcgaca aaagatctta attttggcat
      481 tcgctatcgt tacgactgga aagcttaccg acaacaagac ttatccggtg atatgtctcg
      541 tgataacgtt catcgttggg atggatatgt cacttaccat attaatagtg atttcacctt
      601 cgcatggcaa acgacgctat acagcaaaca gaatgattat cgctatgcaa accacaagaa
      661 atgggcgacg gaaaatgcct ttgttctaca ataccatatg acgcccgata ttacgccata
      721 catagaatat gactaccttg accgccaggg tgtttacaac ggcagagata atttatcgga
      781 aaacagttat cgcattggtg tgtcatttaa actgtagtag acaggagaca gtcacaatga
      841 ataaaactat aatggcgctt gctatcatga tggcttcatt tgccgcaaac gcgtctgtat
      901 taccggaaac tcctgtgcca tttaaaagtg gtaccggagc aattgataac gacactgtct
      961 acattggttt aggtagcgca ggtacagcat ggtacaagct agatacacag gccaaagata
     1021 aaaaatggac tgcgttagct gcattccctg gtggaccgag agatcaagca acctctgcat
     1081 ttattgatgg caatctatat gtgtttggcg gcattggcaa aaacagcgag ggattgactc
     1141 aggtatttaa tgacgtacac aaatacaacc ccaaaaccaa tagctgggtt aaattgatgt
     1201 cgctcgcgcc gatgggcatg gcgggtcatg tgacttttgt acacaacggc aaggcttatg
     1261 ttactggcgg tgttaaccag aatatcttca atggctattt tgaagatctc aacgaggctg
     1321 gaaaagattc aaccgctata gataaaatca acgcccacta ttttgacaaa aaagcagaag
     1381 attatttctt caataagttt ctgttgtctt ttgatccctc aacacagcaa tggagttacg
     1441 ctggcgaatc gccctggtac ggaacggctg gtgcggcggt tgtgaataaa ggtgataaaa
     1501 cctggcttat taatggcgaa gccaaaccag gattgcgaac ggatgccgta tttgaacttg
     1561 atttcaccgg taataattta aaatggaata agcttgatcc cgtctcatca ccagatggcg
     1621 tcgctggcgg ttttgcgggg ataagcaatg attctcttat atttgccgga ggggccggat
     1681 tcaaaggttc acgagaaaat taccagaacg gtaagaacta tgcgcatgaa ggcctgaaaa
     1741 aatcatatag cactgatatt catctttggc ataacgggaa atgggataaa tcgggtgaat
     1801 tatcgcaagg tcgggcctac ggagtatcat tgccctggaa taatagttta ttaattattg
     1861 gcggtgaaac tgcaggcggc aaagcggtga cggattcagt tttaatttct gtgaaggata
     1921 ataaagtcac agtacaaaac taacgcttca gggccccggt aaaggggccc tgctatcaat
     1981 ttgtcatttg tattacgcga aaattatatg aacgcaataa tatcgcccga ttattactat
     2041 gttcttaccg ttgctggtca gtctaatgcc atggcgtatg gcgaaggact gccattaccg
     2101 gacagggaag atgcgcctca ttccagaatt aaacaattag cgagatttgc gcatacgcat
     2161 cccggaggcc cgccatgtca ctttaacgac attattccac tgactcactg cccacacgat
     2221 gttcaggata tgcagggtta tcaccatcct ctggcaacga atcatcaaac acagtacggc
     2281 accgttggcc aggcactgca tattgcacgg aaattactgc ccttcattcc taataatgca
     2341 gggattctta tcgttccgtg ttgccgtggc ggatcggctt ttaccgcggg cagcgaaggg
     2401 acatattcag aacggcacgg agccagccat gatgcttgtc gttggggaac ggatactccg
     2461 ctgtaccagg atttagtcag cagaacgcga gccgcactgg caaaaaatcc gcagaacaaa
     2521 ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa tgaccagtga ctacgcgtca
     2581 caccctcaac actttaatca tatggttgaa gcctttcgta gggatctaaa acaataccat
     2641 tctcagctta ataatattac tgacgcgcca tggttttgcg gcgataccac ctggtactgg
     2701 aaagaaaatt tcccgcattc gtatgaagct atttatggca attatcaaaa taatgtttta
     2761 gccaatatta ttttcgtcga cttccagcaa caaggtgaaa gaggactgac gaacgcgcct
     2821 gatgaagacc cggacgattt aagcacggga tattacggtt cagcgtaccg gtcaccggag
     2881 aactggacga cggcactgcg aagcagtcat ttcagcacgg cagcccgtcg ggggattatt
     2941 tctgacagat ttgtagaagc aattttgcag ttttggcgcg aaaggtgagc ggggagttta
     3001 ttcatggggg atgtccttgt tcgttctcag agtagacaac aaaaaacaaa taagatcaag
     3061 gtaatatctc ttcacttgcg ggctcgaaca gcaggcaatt gccaatataa taatctggca
     3121 caagggtgaa aaacgcgcac cgcataaacc gttgttattg cttacagatt agccggatgc
     3181 cttaatagac atccgcgcct tttcgattat ggctcgga
//
LOCUS       DAZOZY010000304         3216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZOZY010000304 DAZOZY010000000
VERSION     DAZOZY010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.307"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHURQ_004144"
     CDS             <1..495
                     /locus_tag="ACHURQ_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR0825771.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="ACHURQ_004145"
     CDS             525..3041
                     /locus_tag="ACHURQ_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0825772.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3216
                     /locus_tag="ACHURQ_004146"
     CDS             3052..>3216
                     /locus_tag="ACHURQ_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0825773.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          938 a          771 c          731 g          776 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac ggggcaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggag
//
LOCUS       DAZOZY010000305         3205 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZOZY010000305 DAZOZY010000000
VERSION     DAZOZY010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.308"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHURQ_004147"
                     /gene_synonym="dgcC"
     CDS             complement(<1..842)
                     /gene="adrA"
                     /locus_tag="ACHURQ_004147"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR0825774.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHURQ_004148"
     CDS             complement(944..1264)
                     /gene="psiF"
                     /locus_tag="ACHURQ_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR0825775.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHURQ_004149"
     CDS             complement(1383..2798)
                     /gene="phoA"
                     /locus_tag="ACHURQ_004149"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR0825776.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHURQ_004150"
     CDS             complement(2899..3159)
                     /gene="iraP"
                     /locus_tag="ACHURQ_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR0825777.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          749 a          859 c          768 g          829 t
ORIGIN      
        1 ccgcgcaggg taatttgtaa ctgtcgggta agcgccacaa tcgcttcatc gcccacatca
       61 tgaccccagg tatcgttgat gctcttgaaa tggtcgatat cgataatcag taacgttgca
      121 tcgcgattat gccgccgaca gttatcaaat tcattgcgta acatagtttc ccaatgacgt
      181 cggttataca cgcccgtcat gccgtcgcga gtactcatga cctgcaacct gcgtttatgt
      241 tccgccagtt tggttgccgt ctggtagctg acccagccaa acagcagagg ataaacgaca
      301 ataatgggaa gggagagcca ccattccagc ggcgcactat tgaacgacac ggtaatgccc
      361 gtaagctcga gggtgacaag gcaggaaacc accatcaaca ccagacccgc gacaaacaga
      421 cgggggccgc ctgcccccat caaattcaga cacataatca tcaacatagc ggtggaaggc
      481 agcacgttta cgcccattac gcctacccac attcccgcta atactgcatc ggtttttaag
      541 ttgtaaattt cccggctaag cggatcgacg gccctgctcg ctatctgcca ggctaaatgc
      601 ggccagacga acgcccagcc gaccaacacc agccaccacc agcccggcgg cgggtgcgaa
      661 accagcgttg aagcaatcgg taagaacatg ccagccaggc caaccgcacg gggtagtctg
      721 acgcgacggg cgaagcgcag cccggaccgc tgatgttcgt tttgaggagt taaaggaggt
      781 tcctccccat gcgccgccgc ttttttgaaa aagttttcat cattcattat ttttgggaac
      841 attcagaaac aattttccca aattatagag acggggctaa aggcagaagt atgaaatttc
      901 gggtgagcca ctttcgtagc tcacccgtta gcactatcag gtatcaggcg gctttcttga
      961 ggcaggcact cataaactta ttacgatcat cacctttcag agattgttgc gttgcttgat
     1021 tattacattc gcgcatcttt tgctgctgtg gcgtcaaact tttttcacca ggcgcagact
     1081 tgctgttctt caggcaatca ctcatgtagg tcttacgagc atcccctttc aacgcctgcg
     1141 ccgtcgcctg ctgattacag gaggtcatac gctgttgttg tggggttaag gttctctcgg
     1201 cagcgccgac ggtggttaaa aaaaccagac caaaaagcaa ggtaaccagt aatgttattt
     1261 tcatagcacc atccctcttc atgttttaac catgagcgtt tgcgcccgtg atctgccatt
     1321 aagtctggtt gctaacagca aaaaaaccac ccggcagcga aaattcactg ccgggcgcgg
     1381 ttttatttca gccccagagc ggctttcatg gtgtagaaga gatcggtctg gtcggtcagt
     1441 ccaacaacat tggcggcatg cggaccatac gccgcaatac gcaactgact gccggtatgt
     1501 tcttgtgaat cctcttcgga attcccgtaa ctcatcacca tcactgcgcc atctttagta
     1561 tttagcgcct gagtgaggcc cggcgctttg gtatccggcg caacaacctg gctggcgtgg
     1621 gcgtgatcag cggtgactat gaccagcgtg ttaccctcct ttttagcgaa ttccagcgcc
     1681 cgttgtacgg cttcatcgag atcgaccgtc tcgccaattt gcccacaagg attcgcagcg
     1741 tgatcctgtt tatcgattga cgcaccttca acttgcagga aaaatccttt ctcattttta
     1801 ctcaacaatt caatggtttt gtcggtcatc tgtgccaggg ttggtacact gtcattacgt
     1861 tgcggattag gcgtacaggt gactgcgggc ttatcgatat tgccgtggta cgttgctttc
     1921 ggccctagcc agcgcactgg catattgccg tcagcaaaca ggccaagcag gggtttttgc
     1981 tgattcgctt ccgtcaccga attcagtgag gcagcatcgt tcaccaactg ataaccacgc
     2041 gcctgtgcct gttcacgcag cgtttttccc tgccattcac cagcggttgc cgtttcagca
     2101 aaggtttttg cgccgccgcc aagcgtaacg tcggcacgag cgttaagcag ctgttcggta
     2161 atcgatcctt ttccgccttt ttccagagcg ttacccggac atttttcact ggtcgcgctc
     2221 ggaccgtagc atttgcgcga agtcacatgt gccaccagcg cagcgggcgt ggcatcctgc
     2281 aactctgcgg tagaaacgtt accggtcgcc agacctgcgg cttttgccat ttccagaatc
     2341 gttgggtgat ctttttcgtg aatatcgacg cccagcgcgc cgttataggt tttgacaccg
     2401 gttgaccagg cggttgctga tgcagccgag tcggtgacgt agtccggttt gccagttttt
     2461 ttattcagcg catagtgagt gtattgcccg gtaagcggta aggcatcgat acctttaaaa
     2521 aagccgcccg caccttcggc ataattacgt gcggcagtaa tttccgaatc ccccatccca
     2581 tcgccaatca gcaaaataat attttttgca ggtttatcgc taagagaatc acgcagagcg
     2641 gcggtctgat cactcgttaa gcggcgagca ccgccgggtg cagtaatatc gccctgagca
     2701 gcccggtttt ccagaacagg catttctggt gtccgggctt ttgtcacagg ggtaaacagt
     2761 aacggtaaga gtgccagtgc aatagtgctt tgtttcactt tattttctcc atgtacaaat
     2821 acattaaaaa ataaaaacaa agcgactata agtctcggcc gtgacaactt tatgacagct
     2881 gttgaaaaga ttaacttttt actgacgagg atgcttcaat aacttcttta cgtaatcgcg
     2941 cagcagctcc gtatcgtcgt caggaatgct ggcatcgggc tttacctcgt acagcgcccc
     3001 ctctacctga tcaatcaacc gctgttggtc attttgcgcc atattgcgaa gcattgcagt
     3061 gacgataatc tccaaagctt ctacctgcgc acacagttct ttcgactctt cttctttttg
     3121 ggcaagctta aataacaact cagcaatgag atttttcatg tctgtatttc cttatccaaa
     3181 gtatggagaa gttaacatta tgttt
//
LOCUS       DAZOZY010000306         3185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZOZY010000306 DAZOZY010000000
VERSION     DAZOZY010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.309"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHURQ_004151"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHURQ_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR0825778.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHURQ_004152"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHURQ_004152"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR0825779.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHURQ_004153"
     CDS             complement(1715..1984)
                     /locus_tag="ACHURQ_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR0825780.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHURQ_004154"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHURQ_004154"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR0825781.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHURQ_004155"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHURQ_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR0825782.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          772 c          769 g          812 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatc
//
LOCUS       DAZOZY010000307         3073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZOZY010000307 DAZOZY010000000
VERSION     DAZOZY010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.310"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(97..613)
                     /locus_tag="ACHURQ_004156"
                     /pseudo
     CDS             complement(97..613)
                     /locus_tag="ACHURQ_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            complement(684..>3073)
                     /locus_tag="ACHURQ_004157"
     CDS             complement(684..>3073)
                     /locus_tag="ACHURQ_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR0825783.1"
                     /translation="YNRSNVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETAT
                     ELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLR
                     HVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGQGNP
                     VSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLHQRLFRCVSIRENDDGTYAITA
                     VQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKV
                     VKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWGQQGDP
                     ASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETT
                     ARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKG
                     EIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQK
                     VQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLAADRIAMVNPA
                     NGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNPSAFSLTPDGKLTAKNADISGSV
                     NANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVSKSFPKQAGTWGNTETPNGTVTV
                     TISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKNGVLIASRETK
                     GAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKAASGISIR"
BASE COUNT          662 a          938 c          739 g          734 t
ORIGIN      
        1 tctttcagta cacctgaaat ctttactgcc atattcaccc cacaaaaaag cccgcctgaa
       61 ccggcgggct gtcataacac tgtgttacct ggctaatcag aatttataac cgacacccac
      121 gatgaaaccg tcagtgcgcc agagacgtgt tgctgtggcg accgtcatca cttccggtca
      181 gcacatcgtg cgttttcccc ttattgtcag ttacgcggat ataatccccg gaaaaagtcg
      241 acacacggct gtaagccaca cccgccatcg catacgcgct gaaccattca ttcacgcgca
      301 cagacggccc cgccatcacg ctgaaccagc ggttacgcac ggaatcttca tgccagcggg
      361 tatcgctgta acgggtcagc tggcgattct tgtctcctgc atagctgaat gacgtcacca
      421 gccccagcgt atccgtaaac tcataacggt atttcacgtt aatcccgttc agatcatcac
      481 tgccgggaac gttggtccgg gcatgaagat accccgcgct cagcgtggac tgatgttcag
      541 acgcccatgc aggcgcaccg gatacggcca ggcaaatggc tgcggacaaa atggctgcac
      601 aaactttacg cataattacc tctcgctttt ctgcaataaa aaaggcgtca ttcctgacgc
      661 catttattgg ggttataaat atttcaacga atactgatgc cggaagccgc ttttttggtc
      721 acaatcaccg tacagtcggt gatattgcct gccccctgat tgcctttctg gaaaatctta
      781 aactccagag tgacgctacc accaccacta ggcatatcaa taactgcact gtaactaccg
      841 ggaatggcac ctttagtttc tctggatgcg attaatacgc cgtttttgcg aacttcaaaa
      901 ccataacccg tgtatcgcgt gcctcctggg ttatttccgc tccccggatc gtcatacgct
      961 ataccgttaa aaataatggg cggaataata atctggcggt caaagttatg atcatcgctt
     1021 atggtgactg taaccgtccc gtttggtgtt tccgtgttac cccacgtacc agcctgtttc
     1081 ggaaatgatt tggatacagc tttaacgaag tcacctctga cctgagtcgc ctccagcatg
     1141 cccttaatcg tacagttttc atttaccgtg acattgttga gcgtcccggc gttcgcattc
     1201 acactgccac tgatatccgc atttttagcg gtcagctttc cgtccggagt cagggaaaat
     1261 gccgatggat ttccaccgct ggtaatggtg ggggccgtca ggcgtttcag gaacacgtcg
     1321 ttcatgaata tctgatcgcc ctgaccaaca aacatcggtt ttgtgttgcc attcgcagga
     1381 ttaaccatcg caatcctgtc cgccgccagc agcacctgac tctgcatgcc gtcaggggtg
     1441 ttctcaatac cggcaccaat acccgcgata taaaggcgtc cgtcctgcat ctgctgcagc
     1501 ttcacagccc acatgctgtt caggttatta tttgtatcaa cctgaacctt ctgtatctgc
     1561 tgaattgccg cactctggtc ttccagtttc ttattgacgg tctgcgtgat ttcattgctg
     1621 acatccgtaa tggacgtcct gatttcagcc aggtcaggcg caagctgacc gttatcaatc
     1681 tgcgtccaca gctcctgagc cagatgggtt ttccctatct cgcctttgaa aaaatccaga
     1741 taaccggatg catcatcact cggctgacca acagcctcca cgaatgccga tttgccaacg
     1801 gtgttcacac tgcggatata aaagtaataa tcatggcccg gtttgatatt gatactggcg
     1861 gctatccagt acagcgccgt gccaagatag cgtgctgtgg tttcaacctg cctgatatcc
     1921 gcaatccgct tttccgagaa ccagaactca aactgtaccg tcggatcata aaccgcaaga
     1981 tgcggcgtgg cggttatctg aaaatagccc ggcgtcagct caatccgcga cagcgctgcc
     2041 ggtgcggcaa tccggaacga taccgacgcc ggatcgccct gctgccccca cgcatttact
     2101 gcccggactg tcagcctgta gttccccagc gccagttgtg tgaagcggta agtggtttcc
     2161 gtcgtccggg ccgtgctgac cagccgctca ctgccgtcat ccgctgttac ggtcagacgg
     2221 agcaggaagc tcacgccctt caccaccttc ggcgtgtccc agcgcgccag cacctgatat
     2281 tccccgctgt ctgcggtgac ttcggcagtc aggtgctgca ccgctggcgg cgtgacacca
     2341 ttcaccgtgc cgctctggtc gccgtcaaag tgcgccccgt tatccacgat ggcttctttt
     2401 tccggcacat gctgcacggc ggtgatggca tacgtgccgt cgtcgttctc acggatactc
     2461 acgcagcgga acaatcgctg gtgcagcgtc ggcagcttca gcccccacac gctgtattca
     2521 gcaacaccgt caggaacacg gctcactttt accttcacgc cgtcggtgac ggactggacc
     2581 tccacgctga ccggattccc ctgcccgtca accaggctta tcagcgtggt gccggaggat
     2641 ggcagcgtga tttcacggtc gagcgtcagc gtccgggtct ggctgttcac cgccagcacg
     2701 cgcccgccgg tgcggatacc ggcatagtca tcatcgcaga tttcaatgac atcgcccggt
     2761 acatgacgaa gcccttcagc acccacgctg aaatccacgg tctgcgtctc cagcagttcc
     2821 gttttaatca gccacagccc ggcgcggtgt gcctgccccc ggctggtaca gccaaaggca
     2881 tccatcttcg tgacgttacg accgtaacgg acaatggcct gcgtatcctc cacaagctct
     2941 gtcgccgtct cccagccgtt atccgggtca atccagttca cctcaacggc attatggcgg
     3001 tctttcaggg cgctgaaact gtagcggaac ggcgcgccat catccggcat caccacatta
     3061 ctgcggttat agg
//
LOCUS       DAZOZY010000308         3056 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZOZY010000308 DAZOZY010000000
VERSION     DAZOZY010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.311"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            93..425
                     /gene="hdeA"
                     /locus_tag="ACHURQ_004158"
     CDS             93..425
                     /gene="hdeA"
                     /locus_tag="ACHURQ_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR0825784.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            541..867
                     /gene="hdeB"
                     /locus_tag="ACHURQ_004159"
     CDS             541..867
                     /gene="hdeB"
                     /locus_tag="ACHURQ_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR0825785.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            931..1578
                     /locus_tag="ACHURQ_004160"
     CDS             931..1578
                     /locus_tag="ACHURQ_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR0825786.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1620..2150)
                     /locus_tag="ACHURQ_004161"
     CDS             complement(1620..2150)
                     /locus_tag="ACHURQ_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR0825787.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2306..2872)
                     /gene="slp"
                     /locus_tag="ACHURQ_004162"
     CDS             complement(2306..2872)
                     /gene="slp"
                     /locus_tag="ACHURQ_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR0825788.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          891 a          607 c          670 g          888 t
ORIGIN      
        1 atcaacatga catatacaga aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac
       61 ggctctttca ctttatagtt gaggatatta cgatgaaaaa agtattaggc gttattcttg
      121 gtggtctgct tcttctgcca gttgtgagca atgcagcgga tgcgcaaaaa gcagctgata
      181 acaaaaaacc ggtcaactcc tggacctgtg aagatttcct ggctgtggac gaatccttcc
      241 agccaactgc agttggtttt gctgaagcgc tgaacaacaa agataaacca gaagatgcgg
      301 ttttagatgt tcagggtatt gcaaccgtaa ccccagctat cgttcaggct tgtactcagg
      361 ataaacaagc caactttaaa gataaagtta aaggcgaatg ggacaaaatt aagaaagata
      421 tgtaattccg ggaatgcgtt acatcgtact tccttgcata ttgaacaggc cggaatatct
      481 tctttaaaag cagctattcc tcctgttcat atataatctc tatattgaat gggttacaaa
      541 atgaatattt catctctccg taaagcgttt atttttatgg gcgctgtagc ggctttgtca
      601 ctggtgaacg cacaatctgc gctggcagcc aatgaatccg ctaaagatat gacctgccag
      661 gaatttattg atctgaatcc aaaagcaatg accccggttg catggtggat gctgcatgaa
      721 gaaacagtat ataaaggtgg cgataccgtt actttaaatg aaaccgatct cactcaaatt
      781 cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa atttgtatac cttcaaaaat
      841 caagcatcta atgacttgcc gaattaatga ggtacaagta aaaaggagta gcaagttgag
      901 ccatcttgct gctccttttt gcatttttat atgacagcag aatttattat acgtcttata
      961 ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg caaaggagca
     1021 gggttacgca cacatgtatt aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa
     1081 tatggttttg ctgacgtgct gtctttagat cacgtcggac tcgaccccag ccgtattgct
     1141 gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa
     1201 aatattgtag gtctgacgac ggcagcggat atctgggtga ccgccgccat aggtatggtt
     1261 attggcagcg gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg
     1321 gaagtcttcc atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca
     1381 ttagtgaacg gtaatatggt atcaatgctc gactggttta agcagcaaaa aataaaaact
     1441 gatttagttt cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt
     1501 catgccacaa cgtcgataga agacctgttg cgattattaa aaggtatggc aggggtgaaa
     1561 ggcgtttcaa ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt
     1621 cacaccagat aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg
     1681 atcatcatga tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc
     1741 agaatatctt cagttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc
     1801 gaaagtgaca cattcgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa
     1861 tcaatgatag tgatagtccg cggaacaaag gtcaccggta ctttccccag acagcgttta
     1921 atattaaagg gggagaaaac aatgacatgg acaggtttta atcgttcaat ttgagtgaga
     1981 aaattagaat gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc
     2041 aacattacgt cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg
     2101 tttttcatcg cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc
     2161 tggtgcggac attaacttta taatcaattt gccaaaggta attaacagga cagggcttat
     2221 aataataata gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag
     2281 aaagcatctg catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg
     2341 cattggtgta gtaaggcgca ccccagcccg gttccggcca gaatgcacca tagccgtaat
     2401 cccacaggtt ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca
     2461 tattcacttc caggaagtta tacgggactt tattgataaa gccaggttgt tcaccctgaa
     2521 tggtaccgag gatggtaaca aagtgattac gatagttcac tgggtcaagg aagccgcttt
     2581 gtctggcgag cagtcggccc tgatagttgg cttcaatatc aggtttcgca tagctatcca
     2641 acggtaatac agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct
     2701 tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa
     2761 aacttttttg aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg
     2821 cggcaagcaa aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat
     2881 ccttatcaac aaatattaaa accataatag atatttcttt atagttttca tctgattctg
     2941 aggcgatatg cctgatagta acgtaaacct gtctgatgtt tatatccctt ttgtttagtt
     3001 caattccatt gattttgggg cgtggcatga gtatccatcg ccccataata taatcc
//
LOCUS       DAZOZY010000309         3046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZOZY010000309 DAZOZY010000000
VERSION     DAZOZY010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.312"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHURQ_004163"
     CDS             <1..173
                     /locus_tag="ACHURQ_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825789.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            186..1037
                     /gene="hyi"
                     /locus_tag="ACHURQ_004164"
     CDS             186..1037
                     /gene="hyi"
                     /locus_tag="ACHURQ_004164"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR0825790.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            956..2134
                     /gene="allB"
                     /locus_tag="ACHURQ_004165"
                     /pseudo
     CDS             956..2134
                     /gene="allB"
                     /locus_tag="ACHURQ_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2191..>3046
                     /locus_tag="ACHURQ_004166"
     CDS             2191..>3046
                     /locus_tag="ACHURQ_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR0825791.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          696 a          710 c          836 g          804 t
ORIGIN      
        1 aggcgaaagc cttaatggcg caatatcggg taccggtagt cgtggaagtt attcttgagc
       61 gtgtgaccaa tatttcgatg ggcagcgaac tggataacgt catggaattt gaagatatcg
      121 ccgataacgc agtggacgca ccgactgaaa cctgcttcat gcactatgaa taagggagat
      181 aaataatgtt acgtttctct gctaatttat cgatgttatt tggtgaatat gattttctcg
      241 cccgttttga gaaagctgcg cagtgtggtt ttcgcggcgt tgaatttatg tttccttatg
      301 actacgacat tgaagaatta aaacaggtgc tggcgagtaa taaactcgaa catacgctgc
      361 acaatctacc ggcgggtgac tgggcggcgg gggagcgcgg tattgcctgt attcctggcc
      421 gtgaagaaga gtttcgggat ggcgtagcag cagcgattcg ttatgcccgt gcgctgggga
      481 ataaaaaaat taactgtctg gtcggtaaaa cgccggctgg tttcagcagt gaacagattc
      541 acgcaacgct tgtagaaaac ctgcgttatg ccgcgaatat gctgatgaaa gaagatattt
      601 tattactgat tgaacctatt aaccattttg atattcctgg tttccatctc accggaactc
      661 ggcaggcgct gaaattgatt gatgatgttg gttgctgcaa tttgaaaatt cagtatgaca
      721 tttatcacat gcagcggatg gaaggtgaat taaccaacac catgactcag tgggccgata
      781 aaattggtca cctgcaaatt gctgataatc cgcatcgcgg cgaaccgggg accggagaaa
      841 ttaattatga ttatctcttt aaggtaatcg aaaattctga ctacaacggt tgggttgggt
      901 gtgaatataa accccaaacc accacggaag ccggtttacg ctggatggat ccgtactttc
      961 tgaaccgggt cgtagccact gggaaggtta tgaaaccggt actcgcgcag cggcaaaagg
     1021 tggtatcacc accatgatcg aaatgccgct caaccagctg cctgcaacgg ttgaccgcgc
     1081 ttcaattgaa ctgaagttcg atgccgctaa aggcaagctg actatcgatg cggcacaact
     1141 cggtggcctg gtgtcttaca acatcgaccg tctgcatgag ctggatgaag tgggcgttgt
     1201 cggcttcaaa tgcttcgttg cgacctgtgg cgatcgcggt atcgacaacg acttccgtga
     1261 tgtaaacgac tggcagttct tcaaaggtgc gcagaagctg ggcgaactgg ggcagccggt
     1321 gttggtgcac tgcgaaaacg cgctgatctg tgacgcactg ggcgaagaag cgaaacgtga
     1381 aggtcgcgta actgcccatg actatgtggc ttcgcgtccg gtatttaccg aagtggaagc
     1441 gattcgccgc gtgctgtatc tggcgaaagt tgccggttgc cgtctgcacg tttgccatat
     1501 cagcagcccg gaaagtgttg aagaagtgac tcgtgcacgt caggaaggtc aggatgttac
     1561 ctgtgaatcc tgcccgcatt actttgtact ggataccgat cagttcgaag aaatcggtac
     1621 tctggcgaag tgttcaccgc cgatccgcga tctggaaaac cagaaaggca tgtgggaaaa
     1681 actgtttaac ggtgaaatcg actgcctggt ttccgaccac tcaccatgcc ctccggaaat
     1741 gaaagccggc aacatcatgg aagcatgggg cggtattgcc ggtctgcaaa actgtatgga
     1801 cgtgatgttc gatgaagcgg tacagaaacg cggaatgtct ctgccaatgt tcggcaaatt
     1861 aatggcgact aacgcagcag atattttcgg tctgcagcaa aaaggccgta tcgccccagg
     1921 aaaagatgcc gacttcgtct tcattcagcc gaatagcagc tatgttctta ccaatgacga
     1981 tctggaatat cgccacaaag tcagcccgta tgttggccgt accattggcg cgcgtatcac
     2041 gaaaaccatc ttacgtggtg atgtgattta cgacattgaa cagggcttcc ctgttgcgcc
     2101 gaaaggtcaa tttatcctta aacatcagca gtaatctggc ccctgcaatg cccgtccttg
     2161 cggcgggcat tctccggtta aggtgtgttt atgttcaatt ttgcagtcag ccgcgaaagc
     2221 ctgttatcag gatttcagtg gtttttcttt attttttgca acacggttgt ggttcctcct
     2281 acgctacttt ctgcttttca gttgccgcaa agtagcctgc ttacgctcac gcaatatgct
     2341 tttcttgcta ccgcactggc ctgcttcgct caggcgttct gcggtcatcg tcgcgctatt
     2401 atggaagggc cgggtggcct gtggtgggga accatcctta ctatcaccct tggcgaagca
     2461 tcgcgcggga caccgatcaa cgatatcgct accagtctgg cggtggggat tgcactctcc
     2521 ggcgtgctga cgatgttgat tggttttagc ggattaggcc atcgcctggc acggttattt
     2581 acgccgtcgg tgatggtctt gtttatgttg atgctgggcg cacagctgac cactatcttt
     2641 ttcaaaggta tgctcgggct gccgtttggc atagccgacc catattttaa aattcagtta
     2701 ccaccgttcg cgctctcggt ggcggtgatg tgcctggtac tggcgatgat tatctttctg
     2761 ccgcaacgtt ttgcccgtta tggcctgctg gtcggcacca taaccggctg gttgttgtgg
     2821 tacttttgct ttccttcttc gcattcgctc tccggtgagt tgcactggca gtggttcccg
     2881 ctcggcagtg gcggtgcttt gtcgccggga attattctga cggcggtgat tacaggtctg
     2941 gtaaatatca gcaataccta cggtgcgatt cggggcacgg atgtttttta tccgcagcag
     3001 ggcgcgggga atacgcgtta tcgtcgcagc tttgtggcga ccggat
//
LOCUS       DAZOZY010000310         3023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZOZY010000310 DAZOZY010000000
VERSION     DAZOZY010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.313"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            96..1697
                     /gene="aceB"
                     /locus_tag="ACHURQ_004167"
                     /pseudo
     CDS             96..1697
                     /gene="aceB"
                     /locus_tag="ACHURQ_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            1727..3018
                     /gene="aceA"
                     /locus_tag="ACHURQ_004168"
                     /pseudo
     CDS             1727..3018
                     /gene="aceA"
                     /locus_tag="ACHURQ_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT          758 a          780 c          827 g          658 t
ORIGIN      
        1 taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc gttcacagtg
       61 gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca acaacaaccg
      121 atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt actgccgaag
      181 cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat aaacttctgg
      241 cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat tttatttcgg
      301 aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg gacttagaag
      361 accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac gcgctcaacg
      421 ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac tggaacaaag
      481 tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc tataccaatg
      541 aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt cgggtacgcg
      601 gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc ggtagcctgt
      661 ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag ggcagcggtc
      721 cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg agcgaagtct
      781 tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg acgttgctga
      841 ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg cgcgaccata
      901 ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa acgttgaaaa
      961 actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa ccattcctga
     1021 atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt gcgatgggcg
     1081 gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg ctcaacaaag
     1141 taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg atcgctcacc
     1201 caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc cgtaaaaatc
     1261 agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg ctggcacctt
     1321 gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct gtgcagtaca
     1381 tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg gaagatgcgg
     1441 cgacggctga aatttcccgt acctcgatct ggcagtggat ccatcatcaa aaaacgttga
     1501 gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa gagatgaaag
     1561 tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac gatgccgcac
     1621 gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc ctgccaggct
     1681 accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga aaacccgtac
     1741 acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag gcattactcg
     1801 cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg aatgcacgct
     1861 ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac ggtgagtcga aaaaaggcta
     1921 catcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga aagcgggtat
     1981 tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg cggccagcat
     2041 gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg agcggatcaa
     2101 caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc cgggcgatcc
     2161 gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt ttggcggtgt
     2221 cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg cagttcactt
     2281 cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag ttttggtgcc
     2341 aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg tgacgggcgt
     2401 tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca cctccgattg
     2461 cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct tcttccgtac
     2521 tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat atgctgacct
     2581 ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg cacaagctat
     2641 ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt tcaactggca
     2701 gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg atatgggcta
     2761 caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca tgtttgacct
     2821 ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag tgcagcagcc
     2881 ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg ttacttcgat
     2941 aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac cggctccact
     3001 gaagaatcgc agttctaagc aac
//
LOCUS       DAZOZY010000311         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZOZY010000311 DAZOZY010000000
VERSION     DAZOZY010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.314"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHURQ_004169"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHURQ_004169"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR0825792.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHURQ_004170"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHURQ_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR0825793.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHURQ_004171"
     CDS             2805..>2992
                     /locus_tag="ACHURQ_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR0825794.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZOZY010000312         2968 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZOZY010000312 DAZOZY010000000
VERSION     DAZOZY010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.315"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHURQ_004172"
     CDS             <1..314
                     /locus_tag="ACHURQ_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825795.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="ACHURQ_004173"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="ACHURQ_004173"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR0825796.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2201..2917
                     /locus_tag="ACHURQ_004174"
     CDS             2201..2917
                     /locus_tag="ACHURQ_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR0825797.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          807 a          659 c          663 g          839 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc ttaatgattt ccacatatta aaagcaagta tgctttcaaa acacaattat
     2041 aaaaaatccc gccaacaata taagttttta taaaattatg gctttagaat atttttattt
     2101 ctaatagacg agatttttcc tgttatgata taatatgccg aattaacaca tgttaacgat
     2161 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
     2221 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
     2281 tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt
     2341 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
     2401 aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggacgtt atgaaggctt
     2461 tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat
     2521 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
     2581 cagctatatg tttgacaatg ctggtgtgat tgtatttaaa ggatcagacc ctgaccatat
     2641 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
     2701 cattgttatt tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc
     2761 tggaatcact gagttctcaa caacagaatt agaaatgatt gctcaatctg aagttgagct
     2821 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgacga
     2881 tgtacaaaaa gtttatcata acgtcgcaaa tctctaatta tcttttaaag aaatctgtct
     2941 ttacggcaga tttctttaat ctcatata
//
LOCUS       DAZOZY010000313         2956 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZOZY010000313 DAZOZY010000000
VERSION     DAZOZY010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.316"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            74..787
                     /locus_tag="ACHURQ_004175"
     CDS             74..787
                     /locus_tag="ACHURQ_004175"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0825798.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            819..1577
                     /locus_tag="ACHURQ_004176"
     CDS             819..1577
                     /locus_tag="ACHURQ_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825799.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1623..>2956
                     /locus_tag="ACHURQ_004177"
     CDS             1623..>2956
                     /locus_tag="ACHURQ_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR0825800.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          658 a          726 c          803 g          769 t
ORIGIN      
        1 acaataaggt tgaacggata ttctgagcac ggaccacgta atattcaata cattatttac
       61 tgccgggttt ttcgtgaatc aacgtaatat gtcaataatt aattccacgc cagtgcgtgt
      121 tattgccatt gtaggatgtg atggttcagg taaatcgacc ctcacggcaa gcctggtaaa
      181 tgaactggca gcaagaatgc caacagaaca catttatctc gggcaatcgt ccgggctaat
      241 tggcgaatgg atttcacagc tccctgttat tggcgcacct tttgggcgtt atctgcgaag
      301 taaagcggca catgtgcacg aaaaaccctc aacaccgcct ggcaatatta ctgctctggt
      361 tatctatctg ctttcctgct ggcgggcgta caagtttcgc aaaatgttgt gtaaaagcca
      421 gcaaggcttt ctgctcatca ccgaccgcta cccgcaggtt gaagtgccgg gttttcgctt
      481 tgatggcccg caattggcaa aaaccacggg cggtaacggt tggataaaaa tgttaaggca
      541 gcgcgagctg aagctgtacc aatggatggc atcttatttg cccgtattgt tgattcgtct
      601 tggcattgat gaacaaaccg cgtttgcgcg taaacctgac caccaactgg cagcgttaca
      661 ggaaaaaatc gccgttacgc cgcaactgac tttcaacggc gccaggatcc ttgaactgga
      721 tggtcggcaa ccagccgatg aaatcctgca agcgtcacta cgtgcaattc acgccgcgct
      781 ttcctgatca ccaaaatgga taggggatga tttaacgaat ggatgcacta caaacacaaa
      841 ctgttaatag tacaaccgcg ccgcagccca gctacattcc ggggctaatt gcggtggtcg
      901 ggtgcgatgg caccggtaaa tccacgctga ccaccgatct ggtgaaatca ttgcaacagc
      961 actggcaaac cgagcggcgc tatctggggc tgctctcggg cgaagacggc gacaaaatca
     1021 aacgattacc gttggttggc gtctggctgg aacggcgact ggcggccaaa tcctcgaaaa
     1081 cccaaagcat gaaaaccaaa tctccggcgc tatgggcggc ggtgattatg tactgcttct
     1141 cgctgcgaag aatggcgaat ctacgcaagg ttcagcgact ggcgcaaagt ggcgttctgg
     1201 tggtcagcga tcgcttcccg caggctgaaa tttcgggctt ttattacgac ggaccgggga
     1261 ttggcgtcga acgtgcgacc gggaaaatca gcatgtttct ggcgcagcgc gaacggcgtt
     1321 tataccaaca aatggcgcaa tatcgcccgg aattaattat tcgcctgggc attgatattg
     1381 agactgccat ctcccgcaag cctgaccatg actatgccga gctgcaggac aaaatcggtg
     1441 tcatgtcgaa gattggctat aacggcacaa aaattcttga gattgattcc agagcgccct
     1501 acagcgaagt gctggaacag gcacagaagg cggtttctct ggttgccatc gtttctgacc
     1561 gccgaagttt aacttaaggc tggcgggata ctttttacta tcaacgataa ggtcaggctg
     1621 aattggcggg ttttaacatc aaacattggt ttgcagatgg cgcgtttcgc accattattc
     1681 gcaatagcgc ctggttaggc tccagtaatg tcgtgagcgc cttgctgggg ctgttggcgc
     1741 tctcgtgtgc cggtaaaggg atgacgcccg ccatgtttgg cgtactggtg attgtgcaat
     1801 cgtacgccaa gtcgatcagc gattttatta agtttcagac atggcagctg gtggttcagt
     1861 acggaacgcc agccttgacc aacaataatc cgcagcaatt tcgcaatgtc gtctcatttt
     1921 ccttctcgct ggatatcgtc agcggcgcgg tggcgattgt cggcggcatt gccttactgc
     1981 cattcctttc ccattcatta ggtctggatg accaaagctt ttggctggcg gcgctgtatt
     2041 gcacgctcat tccttcaatg gcttcctcca cgccgaccgg cattctgcgt gcggtagatc
     2101 gcttcgattt aattgctgta cagcaggcga cgaaaccttt tctgcgcgca gcggggagcg
     2161 tcgtagcctg gtattttgac tttggttttg cgggttttgt tattgcctgg tacgtgtcga
     2221 atctggttgg cggcaccatg tactggtggt ttgcagcgcg cgaattacgc cgcagaaata
     2281 tccataatgc cttcaaattg aatctgtttg agtctgcccg acacatcaaa ggcgcgtgga
     2341 gttttgtctg gtcaaccaac attgcccact ccatctggtc ggcgcgtaac tcgtgcagca
     2401 cagtgctggt ggggatcgtg ttaggacccg ctgccgccgg gttatttaaa atcgccatga
     2461 cattcttcga cgccgccgga acgccagcgg gtttgctggg taaaagtttt tacccagagg
     2521 tgatgcgttt agatccgcgc accaccagac cgtggttgtt aggtgtgaag tccggtttac
     2581 tggcgggcgg aatcggtatt ctggtggcgc tcgcggtgtt gatcgccggc aagccgctca
     2641 tttcgctggt gtttggcgtt aagtatctcg aagcgtatga cctgattcag gtgatgctgg
     2701 gcgcaattgt gatttcgatg ctgggtttcc cgcaggagtc attgctattg atggcgggaa
     2761 aacagcgcgc ttttctcgtg gcgcaaacca tcgcctcaat cggctacatc gtattgctgt
     2821 tcatgttctg tcatctgttt ggtgtgctgg gagcagcgtt tgcttacttc ggtgggcaat
     2881 gtctggatgt ggtgctctcg ttaattccaa ctttaaaggc attttttcag cgtcattcct
     2941 tgctttataa cgcagc
//
LOCUS       DAZOZY010000314         2939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZOZY010000314 DAZOZY010000000
VERSION     DAZOZY010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.317"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHURQ_004178"
     CDS             37..264
                     /locus_tag="ACHURQ_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR0825801.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHURQ_004179"
     CDS             complement(265..639)
                     /locus_tag="ACHURQ_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR0825802.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHURQ_004180"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHURQ_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHURQ_004181"
     CDS             complement(2099..2674)
                     /locus_tag="ACHURQ_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0825803.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          762 a          695 c          689 g          793 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgaccaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttca
//
LOCUS       DAZOZY010000315         2919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZOZY010000315 DAZOZY010000000
VERSION     DAZOZY010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.318"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(94..1581)
                     /gene="yeeO"
                     /locus_tag="ACHURQ_004182"
     CDS             complement(94..1581)
                     /gene="yeeO"
                     /locus_tag="ACHURQ_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR0825804.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1742..1817
                     /locus_tag="ACHURQ_004183"
     tRNA            1742..1817
                     /locus_tag="ACHURQ_004183"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1775..1777,aa:Asn,seq:gtt)
     gene            complement(1855..2805)
                     /gene="cbl"
                     /locus_tag="ACHURQ_004184"
     CDS             complement(1855..2805)
                     /gene="cbl"
                     /locus_tag="ACHURQ_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR0825805.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          855 a          781 c          615 g          668 t
ORIGIN      
        1 gaacgaggcg aatattagcg atgcccaccc acaatgtcaa agcctgtttt ttaaatttga
       61 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacaactg gctttttttc
      121 acacttttgc ggttcggatc gagggtattt ccacagccaa cgtccagtaa ccattcgcca
      181 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
      241 accccagcca agcatgatgc ccagcacata accgacgaca acccggcaac cccacatgct
      301 caacatagaa acccacatgg cgtaacgggc atcgcgagca cctttaaatc cagcgggtag
      361 cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca gaatcacaac
      421 gacatgttta acctgtggat cctgggtgta aaacgatgcc ataaccccgg caaagggagc
      481 cgttagccag gcgatggccg ttaatccaag agtggaaagc cagaacacat gccgcaactg
      541 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
      601 agagccgagc gcacttccgg gtaagttgat aagagccgca attgaaaacg cgataaaatt
      661 tccggcaata acactggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
      721 ggtaaataac actgattcga cactcgcggg aataccaatc cccatgactt cccagataat
      781 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
      841 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccag
      901 ccctgccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca atatgccgct
      961 aataataata ttaagaatat tcaggctacc gttaatcaat agcggtattt tcgtattccc
     1021 tgcaccacga agtgccccgc taccaataag agtgatggca gctgctggat aactgagtac
     1081 cgtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac
     1141 gaaatcaata atttgttcgc caaaatgatg aataagcgtt gccaacagta cggcaaacaa
     1201 cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc
     1261 gagactaaat gccacaacga cagtagtacc aagatcgata gcagcaaaaa aagccataat
     1321 gaccatattg aagctgtccg ccaatcccac gccggccatc gcatcttttc ccagccagct
     1381 gaccagaaaa gtgctcagaa cccccatcaa caggacacag gcattctcca tgaagatagg
     1441 aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagcgtg
     1501 ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa
     1561 ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt
     1621 ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca aacagttggg
     1681 gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc tcaacagcga
     1741 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     1801 tccagtcaga ggagccaaat ttctgaaaag cccgctttta tagcgggctt tttgctatat
     1861 ctgataatca atttcctctt cactgttttc catcacctgc cgcttgatat cctcaactga
     1921 cagtcctgca ttacaaagtt ccagaaagcg ccagacatag ttacgctgaa gttgtcctcg
     1981 cttcagtccc aaccagacag tattagcatc gaaaagatgc cgcgtatcca gacggattaa
     2041 attctcttcc tcttgttcgc cgctggattg ttcggcaact aatccgatcc caagcccaag
     2101 agcaacatag gttttaatga catcagaatc ctgcgcactt aatacaatat ctgccagcaa
     2161 acctttgcgg gcaaatgcgt catcaatacg tgagcgcccc gtaatcccct gtcggtaagt
     2221 gattaacggc cacttcgcta ttgattccag cgtcaatggt gtaatttgcg ttaagggatg
     2281 atcgagtgga acaagcaaac tatggtgcca acgaaaccac gggaaggcga cgagctgcgg
     2341 gtcattactc aaacgctcgc tggcgatacc aatatcagct tcgccatttt gcaacaacgt
     2401 cgcaatttcc tgtggcgtcc cctggattag ctcgagccga acctccggga aaagttcgcg
     2461 aaaagcttta atgacctctg gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt
     2521 gagaacgcca gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat tactggcttc
     2581 attcagaata cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca tgcccagcag
     2641 tcgcttacct cgtcgaacaa atatttcgat gccaagttca tcctccagtt cccgaatatg
     2701 acggctgacg ccggactgtg aggtaaaaag catattcgca acctctgtca ggttgtaatc
     2761 ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa actccgggca
     2821 gttcagattt cccgttattg ttaaagtcta atgcccggca taacaaataa taaaaacccg
     2881 catcttattc catcccgata taacgcttag ctcaccaat
//
LOCUS       DAZOZY010000316         2919 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZOZY010000316 DAZOZY010000000
VERSION     DAZOZY010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.319"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHURQ_004185"
     CDS             <1..495
                     /locus_tag="ACHURQ_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR0825806.1"
                     /translation="GADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPA
                     PDESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNAL
                     MLRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDP
                     ARSK"
     gene            495..1085
                     /locus_tag="ACHURQ_004186"
     CDS             495..1085
                     /locus_tag="ACHURQ_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR0825807.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1834..2112
                     /locus_tag="ACHURQ_004187"
                     /pseudo
     CDS             1834..2112
                     /locus_tag="ACHURQ_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            2172..2660
                     /locus_tag="ACHURQ_004188"
     CDS             2172..2660
                     /locus_tag="ACHURQ_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR0825808.1"
                     /translation="MTKQYDVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVS
                     ARTGEWEEDLLLGLISDEDREKLKAYRIYAKSLQAMDFSTITDKSSYNAIEWSVSPEG
                     SS"
     gene            2684..>2919
                     /locus_tag="ACHURQ_004189"
     CDS             2684..>2919
                     /locus_tag="ACHURQ_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR0825809.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT          758 a          636 c          833 g          692 t
ORIGIN      
        1 ggcgcagacc ctgattatgt tatctgggcg aaagaagttc agggtattaa ccgtgcatgg
       61 actttcagac actggaaagg cactggaacg gttggcgtga tggtggcgac aaacgatccg
      121 gaacgcccgg ccccggatga aagcgtgatt aacgcagtca gggaccacat ccttcctctg
      181 gcacctgttg ccggaagcgg attgtatgta ttcggtgcca cagaaaaagt catcccgatg
      241 acgattgcat tatcgaaaga cacaccgcag atcaggactg caataaaagc agagctgaat
      301 gcactgatgt tacgggatgg tgtgccagag gggcgtatgt atctgtccag aatcagtgag
      361 gccatcagtt tgtctgctgg tgaagtggcc caccggttaa tcgtcccttc atcggatatt
      421 gacctggggg agaccgagct tccggtgctc ggcgagatca cctggcaggc ttatgacccg
      481 gcaaggagta aataatggat tcgttacagg atgattatac aaagctgctg tacggactga
      541 tgccgccggg gcctgcatgg tcagataccg acggtgtact tgacggtctg gcaccatcgc
      601 ttgtgcgtgt tcaccagcgg gctgatgaac tggtgattga aatcgatccc ggtcagtcca
      661 cagagctgat tgaacgttat gaagaattgt atggtttacc tgattcctgt tcccctgtcg
      721 gaacccagac attacgccag cgtcagcaac gtcttgaagc aaaagccaat gtggctggtg
      781 gcataaatga gcagttcttc ctggatcagc ttgaggcgct gggatatacc ggcgtaacga
      841 tcgaacagtt ccagcacttg gatgcaagcc ccgatccgga atggggagat cgctggcgtt
      901 atttctggcg tgtgacgttg ccgatggatg ccggtgctca gtggcagaca tgcacggacg
      961 cctgcaacac accgatccgg acgtgggggg atacggttgc ggagtgcgtg attaataaat
     1021 tatgtccgtc acacaccgtc gttttatttt cctatccaga tgaggatgaa gatgcacagg
     1081 attgatacgc tgactgcggt aaaagataag tttggtccgg gtaagaacgg atttactgac
     1141 ggaaatcttc gcacaggacg tcttgctacc tggctgaaca gtgccatgtg gaatgccatt
     1201 caggaggaaa tctgtggtgt cattgagaag gccgggatag aactgaataa agaagaacac
     1261 gaccagctat ataaggctat attattgctg gtgggcggtg caattaacga agaggcattg
     1321 ctgataaaaa ataacctttc ggatgtggaa gacagggatg aggctgttga aaacctcgga
     1381 ttaaaaccca cggtggataa ggcaaaaaat gccgttcagc gtgatggcga caccatgacc
     1441 ggagagctga aaatccgtgg tgttaatgcg ctgaggattt tcaacgaagc ttttggcctg
     1501 atttttcgtc gttcggaaga gtgcctgcac cttattccca ccagtgaagg tcagggggaa
     1561 aatggcgata ttggtcccct gcgcccgttc accattaatc tgcggacggg tgaaatatcc
     1621 atgtcgcata aagtgtctgt tggcggtggt tcgcaggtca atggtgcgct gggtatcggc
     1681 gttcagaacg ccctgggggg gaattcaatt gttcttggtg ataatgacac cggattcaaa
     1741 cagaatggtg atggcctgct ggatgtttat gccaatagcg tgcatgtgtt gcgttttcag
     1801 agtggcagta tccagagtaa taaagctgta aacgttacag gacgggtaac accgtcagac
     1861 tacggaaact ttgatgcccg ttaccagcag cgaaatggtg gcgtgcagga tgtgcggtat
     1921 ggttatgaaa tgtattacac ccccggcagt aacaccgttt cgtggacatt tcgctcacct
     1981 tcgggacacg ggctgtcagg gatatcgata tcggataccg gccgtaactc agcggataac
     2041 gtcaacggcg tgtattaccg accgctgcaa aaactgatta atggcacctg gtataacgta
     2101 gcgagtattt aacaatgttg catttaaaaa atattacggc aggtaatccg aaaaccgcgg
     2161 aataatatca gctgacaaaa cagtatgatg tcacctggct tttttcggaa gacggcaaaa
     2221 actggtatga agaacagaag aactttgcca gtgacaccat aaaaatggtt tacaccggag
     2281 acgggcgcgt ggtgtgggtc ggtaaggatg tgacaggcat tgaaccccgt aacgccagtg
     2341 ttattgaagt tcctgatatt accgccaacc gccgcattac cgcgccgggt tactggtttt
     2401 accgcaatga tgaatttgtc tttgactaca gactcaaagc ggaagatgag cgtgatgccc
     2461 ttctggctca ggtcagtgcc cggacagggg aatgggaaga agacctgctg ctggggctaa
     2521 tcagcgacga agaccgggaa aaactgaaag cgtaccgtat ttacgcgaaa tcgctgcagg
     2581 cgatggattt cagcaccatc actgataaat cctcatataa cgccattgaa tggtccgtct
     2641 ctccggaagg ttcttcctga tttaatttat cgcgagaaaa actatgtctg tagtgatatc
     2701 aggtgcgctg actgatggcg caggcatccc catgtccgga tgccagatta ttctgaaatc
     2761 ccgtgtaaac acctcagaag tggtgatgcg taccagggct gatgtagtga ccggaaataa
     2821 cggtgaatat tcgtttgagg cacaggtcgg aaaatattgc gtgtatctga aacgggactg
     2881 gcgcgacgag tactgtgttg gcgacattgc tgtatacga
//
LOCUS       DAZOZY010000317         2900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZOZY010000317 DAZOZY010000000
VERSION     DAZOZY010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.320"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHURQ_004190"
     CDS             complement(<1..1199)
                     /locus_tag="ACHURQ_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR0825810.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHURQ_004191"
     tRNA            1492..1586
                     /locus_tag="ACHURQ_004191"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2900
                     /locus_tag="ACHURQ_004192"
                     /pseudo
     CDS             1858..2900
                     /locus_tag="ACHURQ_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAZOZY010000318         2878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZOZY010000318 DAZOZY010000000
VERSION     DAZOZY010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.321"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHURQ_004193"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHURQ_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR0825811.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHURQ_004194"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHURQ_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR0825812.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHURQ_004195"
     CDS             1650..2099
                     /locus_tag="ACHURQ_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR0825813.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHURQ_004196"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHURQ_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR0825814.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHURQ_004197"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHURQ_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR0825815.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHURQ_004198"
     CDS             2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHURQ_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR0825816.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          757 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttat
//
LOCUS       DAZOZY010000319         2830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZOZY010000319 DAZOZY010000000
VERSION     DAZOZY010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.322"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..456)
                     /locus_tag="ACHURQ_004199"
     CDS             complement(<1..456)
                     /locus_tag="ACHURQ_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825817.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASLKSELQSLTFWKVSPK"
     gene            complement(589..900)
                     /locus_tag="ACHURQ_004200"
     CDS             complement(589..900)
                     /locus_tag="ACHURQ_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825818.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
     gene            1093..1644
                     /locus_tag="ACHURQ_004201"
     CDS             1093..1644
                     /locus_tag="ACHURQ_004201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825819.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
     gene            2395..>2830
                     /locus_tag="ACHURQ_004202"
     CDS             2395..>2830
                     /locus_tag="ACHURQ_004202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825820.1"
                     /translation="MKITVDNISEMFAGTKELLIGGLITGIVGVLILTRVSGYEYVKV
                     PLMLVMIFLLTVVPGFIAMLFSPLTKVFAKIFDIKSSNILCRIFSFLYFILYMAITFL
                     VTNFIFTTAMSLHGGDGGLRACFAIGCFVVAVEQTRKFYSLLI"
BASE COUNT          840 a          527 c          602 g          861 t
ORIGIN      
        1 ttttggcgat actttccaaa aggtaagtga ttgcaattcg ctttttaatg aggctaactt
       61 aatgtttaat agatcgataa cttctcgttc agttttaact aaatgtgcga aattttttgg
      121 ttctatatct ggttcagtat tgttaaggtt gcatatattt tcatatgttt tcaacatctc
      181 caacgaaaaa agatcgcaat tagtccagaa cttagtaact tgatttaaag cattttcctt
      241 tattttagtt tgaaaccaat ttttagcgtt ccatgctgca tatagggctg ctccagccat
      301 aatgagattg gcaatagaac taacccagtc actaaccgat ccccactcga aaccttcaga
      361 accaacaaag agtgatttta ttagtactaa aattacaaca accagtaata ctgctacggt
      421 cgataaaaga acgatattta attttctaca atccatcgaa agtagttcct gaacgcatta
      481 aaggggcaaa tactttacca tccgaaacac aatcaagtac actacccgtt cacgtactgc
      541 tcacatgttg ggtttgttat tttgttgggg tgagcagtac gtgtatcctc agaagggtag
      601 ttccccctga tcacagaacc aatcaccttc ttcactgctt tgattcatcc ggtactcagc
      661 agatagatat gctaacccat caataacact tattagtggt gtttcacagg taagtacaaa
      721 acaatctctc tctatagtac ctaaccagta ttccctgtac tgattctgga agaataccct
      781 atccccataa tgaaattctg ttagtgcaga tccccaataa cacacggttg cccagcgacc
      841 gttatgtgag aacttctgaa cccctcgcgt aatggatgga tcatagccgt ctttgctcat
      901 ttgttaccct cctctatgta ttactgtata tatgtacagt attgcagtca gtttcccaaa
      961 aatccagcac ccctttgttt accactttga atgattaatt ctattgtaat gatcaataaa
     1021 attgatcgat ttaattgcca atgttttaaa aatgatcaat tatcaataac ttaaagcgta
     1081 acgagcaaag ttatgaacat tgataattgg gcaataccga taacaagttt gggtggtcag
     1141 ttgctggggg ttgagttggt cagccatgta gaacgcgagg gaatcaggca gatcggccta
     1201 tgggaagaag aagtacttca tgggcaacta atgcttattg aaagcaaggc ggcatggttc
     1261 agggataacg gtttgtattg tgtgattact actggccgtg gtgaaccata cgagtactta
     1321 ccattccttc ggcagatgac aagggatatg aaagtacata accagcagtg gcttgatgat
     1381 ctcggtgtac atggtggttc tgctattcct ctggttacag gtgatttcga ggttgcccga
     1441 ttaaatagaa ggtacacaga agagaacatt gaccgtctga tctttccagt actcataaaa
     1501 agcattcgcc agtactgcga gaaggtgatc gtacccgtca gagataaaac gtattaccca
     1561 attctcagag aggccggtgt atgggcggta cagggagagt tcaggccaat gcgttttgag
     1621 caatgtgaaa aattaatagg ataacaataa tgaaaaaatt actaattggg ttactgattg
     1681 cccttagtac tggatgtact actacgcaca gcttcatgga taaagcttac gttggctatg
     1741 tagatgacaa ggccgtagta ctgtttgatg caggaacacg taatggggtg aagtactacc
     1801 gtcctcagtt ccagagtgat tgcggtcgca tctataacaa gatctatcat gatgacaata
     1861 caagtactgg atgcctcata cgagattccc gcattgctga actacatccc tatagagagc
     1921 atgtaagtaa ggtagtacca catcagaccg atagtgttca cgattggtta gaaggggtca
     1981 attcaccatc ccatatcctc gacaatctat agggtacgta tgggaaaggt aatggtgatt
     2041 ttgttagtac tggctggggt actcttcaac attgcaggtg aggatgcacc ccagccacag
     2101 tacacggaca atgagaacca gaacgctatg gatttcgcac agtcactaat caagggtaag
     2161 tacccatgcg atatggtgac tatggatagt actgagaatg gtacaggacg tattgatgta
     2221 cggtgtgatg gtaagtacca gtatgagatc atccggcatg atggacaatg gcaggtagta
     2281 tcgaagtaat ccgaaaagtt tagtgcttag gctgtatggt gagaatctac agggcttttt
     2341 ctttatgcgg tgataagatc ggagtgaact ttttatgaag gaatagttag tagaatgaaa
     2401 attacagtcg ataatatctc tgaaatgttt gcaggaacta aagaactact aatcggtggc
     2461 ttaataacag gtatcgtcgg agtacttatt ttaactcgtg ttagtggtta tgaatacgtt
     2521 aaagtacctt taatgttagt aatgatattc ttactaactg tagttcctgg ttttattgct
     2581 atgctttttt caccactaac gaaagtattt gcaaagatat ttgatattaa aagcagtaat
     2641 atactttgcc gcattttctc atttctatat ttcatcttgt atatggctat tactttcttg
     2701 gtgactaatt ttatattcac taccgcaatg tcattacacg gcggggatgg tgggttgaga
     2761 gcatgttttg caattggctg ttttgtcgtt gcagttgagc agacaagaaa attctatagt
     2821 ttgcttatca
//
LOCUS       DAZOZY010000320         2820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZOZY010000320 DAZOZY010000000
VERSION     DAZOZY010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.323"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            53..607
                     /locus_tag="ACHURQ_004203"
     CDS             53..607
                     /locus_tag="ACHURQ_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0825821.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(617..2044)
                     /locus_tag="ACHURQ_004204"
     CDS             complement(617..2044)
                     /locus_tag="ACHURQ_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR0825822.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2041..2748)
                     /locus_tag="ACHURQ_004205"
     CDS             complement(2041..2748)
                     /locus_tag="ACHURQ_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR0825823.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
BASE COUNT          814 a          671 c          620 g          715 t
ORIGIN      
        1 tgagtataaa cttgacgctt tcaaaataaa aagaaaatcg aagcattcac acatgaataa
       61 aaaatcaatg tatatattcg caatttttat agttgcagca attacctgta ttagccaacc
      121 caagaaaacg acgttgcgtg ataaagccat ggtgaattat gcctttgatt atttaagatc
      181 accaggcagt ctaccgttaa ccacggcagc cacggagctt tccgcgattc atggtcactc
      241 aacatcgcaa tatcgccttg gcgaatttta tcttcatggt agcgacggta aaccactgga
      301 ttatacacag gcgagatact ggtatgagca atcagcagaa caggaaaatc cgcgcgcaca
      361 aagcaaactg gggtggatct acctgaaagg cctgggagtc aaacccgata cccgtaaagc
      421 aattctctgg tataaggaag cagctgaaca agggtatgct catgctcaat atactttggg
      481 tttgatctac agaaatggca ctggtattaa tgttaaccat tatgaatctc aaaaatggtt
      541 aaaactggcc gccaaacaac attacaaaaa tgcggaaaga ttacttgccg gacttcccgc
      601 acattaacgt cgactgtcac ccgaagagat ggagtatttt tcctatcaaa ccggcaaaga
      661 taaaaatgta gaaaataatt ttgatgacgc ccagacagcc cagcccgcca tcgtcaacgg
      721 gcattggctg cggttgcggc tccggctcga cggtttcctg accggtaaaa ttcgccgcct
      781 gaattaataa tcgacgaata attaatgctt ccagtggaca acgatcttct gcatcaaggc
      841 ggctcacaat cggcattaat gattcttctg tcggttggct ggcctgcact aattgtttgg
      901 caactaaata taatggccat aagggatgtt ctgtatcacg cgtgagtgag atccgcggct
      961 tacgtccttt gtgcaaaacc atctcgacca gtgaacgccc cagcgcagag agtttttcat
     1021 cgctgagtat ttcgtataaa tgaaaacgcg tacgggcgct gtcgccgggg acataaagta
     1081 gctgtttacc ctgatcgtta accagttcac gagcttcaat catcatcacc actaacgggt
     1141 aataatcgac ccgatgctga cggcaccatt gcaatattaa agcatccgcc tgggtggagg
     1201 gatactcgcg ccagtagtca caaagttctg ccagcaagct ttcgccttct ccacaataga
     1261 cgcgttgcgc gtattcataa taacgcgcat cttcattatc aggttcagct tcccgccacg
     1321 accgggcgta atccagaagt tcggggatat ccaggcgacc tgcgctatac cagcggagta
     1381 aatttctgtg cagttttgca tcatcgggga tgatcactgc gcgatgttgc gctaaataca
     1441 atgtcagcca ttctggatgc gatgaccaaa tccgagcgaa catttggtaa aaatcgatgg
     1501 tgtcattttg cacggcaacg ggcagatgca gaatggtctg gaagttaaaa acattcccct
     1561 tcgcaatggc atataaaaag gattccagtt cttcttcgtc tatttccccg acgttttcct
     1621 cctgcaacca ccgcaatctt tctgctaata acaccacgca ctcgaaggat aaatgggcag
     1681 tgttcatggc gattgtgcac agcgaccagc gtaattcatc aatctcttcc atcgagcaat
     1741 aatttaattg ctgaatgaat cgctgccagg cggagggcag aaaacgttcg ctatcagagg
     1801 caagtaacgc gcgaaaggcg agcagaattt catgttctgc tacctcatat tcttctggtt
     1861 gccaaacaga tttagcaggc gactgcgcaa tccggagcgc ctcctcatac gcctgacgaa
     1921 gttgtttaaa accctgcgga tcggtttctg gatgaaagga cggtaacagc gccagataag
     1981 cgcggcggat aatatcgacg tcagtcgttt cctctatttc gaggatcttc cagcaatttt
     2041 tcataggtcg ctcatatcct taagcgtttc cgggcatgtg gggtagtcag ccagtggttg
     2101 ccaggacaag gcagggtaat acgcatcatc actggcaaat aattgataaa acatatcatt
     2161 gccggaatcg tcgtattctt tacgagctaa gaattcgcgt aataaaggca gatgatcatc
     2221 ttcgggagac tgccaatatt gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt
     2281 actataataa cgtagagccc gtagatgaat acgcgactgg atccatagac tttcttcttc
     2341 actcaaccag ttattaagta cgcccattcg acttaaaaaa cccatccgga cataatccca
     2401 tgctttaatt cctccctcac cacagcattc ggctgtgctg gcgacaaatt gcgcataagc
     2461 ctgaccttgc tcatttagtt cagcaagata gtcacgccat tcacatggcg aataacgaaa
     2521 ccagcggtga taaaaccctg ccaaccgagc ggcatgaccg ttatcgatca ttcggtgaat
     2581 tgtgcgcagc aggtcatcac ggtcatcaat tccccatgag gaagaaaggt caaaaaagca
     2641 tgtacctaaa tacattttgg gggatgaatg cgaaggtaaa cagcattcat tgcgtatttc
     2701 catcggagtg gaaagtgcaa acaatatttt ttgcgacccc atgtccatat aatttatcct
     2761 taaacattca tttaatgaat gtaaactaca tttcgagact cagttattta tacttttctg
//
LOCUS       DAZOZY010000321         2797 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZOZY010000321 DAZOZY010000000
VERSION     DAZOZY010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.324"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..617
                     /locus_tag="ACHURQ_004206"
                     /pseudo
     CDS             1..617
                     /locus_tag="ACHURQ_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
     gene            614..1093
                     /locus_tag="ACHURQ_004207"
     CDS             614..1093
                     /locus_tag="ACHURQ_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR0825824.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1147..2097)
                     /gene="corA"
                     /locus_tag="ACHURQ_004208"
     CDS             complement(1147..2097)
                     /gene="corA"
                     /locus_tag="ACHURQ_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR0825825.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2249..2305)
                     /gene="ysgD"
                     /locus_tag="ACHURQ_004209"
     CDS             complement(2249..2305)
                     /gene="ysgD"
                     /locus_tag="ACHURQ_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR0825826.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2467..>2797
                     /locus_tag="ACHURQ_004210"
     CDS             2467..>2797
                     /locus_tag="ACHURQ_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825827.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          842 a          620 c          591 g          744 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagtatcaat taacacaaga aaataaaata gagcgagttt tcaatccgca
     1081 tgactttaaa taagacaatt tttagaaata agccggataa aaaaatagct ttatccggca
     1141 ctcatattac agccagttct tgcgcttaaa gtacagatac ggtgccaggc ccgcgaggat
     1201 cataaagata atcgcgcctg ggtagccgaa actccacttc agttctggca taaactcaaa
     1261 gttcatccca tagctggaag caacgagcgt cggcggcagg aataccacgg ataccaccga
     1321 gaagattttg atgatgcggt tctgctcgat gttgataaaa cccatcgccg cctgcatcag
     1381 gaagttcacc ttctggaaca gggattcgtt atgcggcagc agggattcga tatctcgcag
     1441 gatttcacgc gcctgctcca gttgcccacc cggtaaacgc gctttacgca ccaggaagtt
     1501 gagcgcgcgc tgggtatcca tcagacacag gcgaactttc cagccgatat cttccagttc
     1561 cgccagagtg gagagcgcct cgtcgtactc atcgccctga tgcccttcca taatcacccg
     1621 gctcaactgc tccaggtcgc tatagatatt ttcaatttca tctgccaact gttcgatttt
     1681 ggtttcgaac agatccagca gcagctcgta ggcgttaccg tctaccatcg actggctacg
     1741 ggcacgcata cgatacagac gaaaagcggg cagttcacgc tcacgcagag taaacagacg
     1801 accatcacgg atggtaaatg ccacagtgga gttaccggcg tgatcttccg catcttcaaa
     1861 gaagaagaag gagtgaatat gcaggccgtc gtcgtcttca aagaaacgtg ccgatgcttc
     1921 gatgtcttcc agttccgggc gggttgccag gctctggcca agttcagatt gtacgcgcag
     1981 tcgctcgtcg tcgtccggtt cgacaagatc aatccatact gcatttacaa ggggttgtga
     2041 ctcttcgact tccagccggg tcagtcggtt attttccagt tgaaatgcgc tcagcatgac
     2101 cgggactccc aatgcttaaa atatcggaca gttcggtggg cacacagaaa caaattgggt
     2161 ttcagaccat taaacagcct gactcagcgc gacgggaaaa attgaggtcg ctgacaaccg
     2221 ctaaggctat cagcaaaaag ggatagcctt aggagttgat cctggatgac aggatagtga
     2281 gccagtatct actgggtgtg tccaaggcga atgtcctctt agagtgatcg tgcgcgcatg
     2341 ttacgccagc aaaattttgc cgtcaacacg caacagaaca ccacagaaca atagttgaac
     2401 ttcgcgcttt ttggtactcg tcaaggtcgc taacaggata aaaaaatcca acatccagga
     2461 cctataatgg cgcatcggct actcattggt aaaggaatga tcaccttgaa tctcaaacgg
     2521 atttttctcg ccctcacctt acttcccttg tttgctgttg ccgctgatga ttgcgcactc
     2581 tcagatccga tgctgaccgt acaggcgtat accgttaatc ctcaaacaga gcgggtgaaa
     2641 atgtactggc aaaaagccaa tggcgaagcg tggggaactt tacatgctct gctggtggat
     2701 atgaatagtc agggtcaggt gcagatggcg atgaacggcg gcatctatga tgaaagctat
     2761 gcgccgctcg gtttgtacat cgaaaacggt cagcaga
//
LOCUS       DAZOZY010000322         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZOZY010000322 DAZOZY010000000
VERSION     DAZOZY010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.325"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHURQ_004211"
     CDS             complement(423..680)
                     /locus_tag="ACHURQ_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825828.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHURQ_004212"
     CDS             complement(677..1174)
                     /locus_tag="ACHURQ_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR0825829.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHURQ_004213"
     CDS             complement(1376..1834)
                     /locus_tag="ACHURQ_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0825830.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHURQ_004214"
     CDS             complement(1831..2328)
                     /locus_tag="ACHURQ_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR0825831.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHURQ_004215"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHURQ_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR0825832.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          649 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaa
//
LOCUS       DAZOZY010000323         2703 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZOZY010000323 DAZOZY010000000
VERSION     DAZOZY010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.326"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..247
                     /locus_tag="ACHURQ_004216"
     CDS             <1..247
                     /locus_tag="ACHURQ_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR0825833.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
     gene            complement(287..1495)
                     /locus_tag="ACHURQ_004217"
     CDS             complement(287..1495)
                     /locus_tag="ACHURQ_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0825834.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHURQ_004218"
                     /pseudo
     CDS             1503..2004
                     /gene="tnpA"
                     /locus_tag="ACHURQ_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2022..2690)
                     /locus_tag="ACHURQ_004219"
                     /pseudo
     CDS             complement(2022..2690)
                     /locus_tag="ACHURQ_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          625 a          665 c          666 g          747 t
ORIGIN      
        1 ggctgccctg cccgtaagtt ggatccagat gctggcaggt ctggcgctgt tgagtaccat
       61 tggcggcagt ttgtatcagg cgctgcataa tgagcgtgag cgtgagcgcg acgcggcggt
      121 ggtggcattt ctggtaacgg caagtggatt gacgctggtc gggattggtt ctgcgttctg
      181 gggattaatt gccggaggcg tttgttacgt ggtgttgaat ttaatcgctg acagaaaccg
      241 atattgacat cctccacgcc ctgaaggacg tggatccctg ctacgttcag gctgtcgcct
      301 gaatcatttc ggtgggttcc tgcttcaacg ggcggcctga ctgcaccatc tctccacagg
      361 caagaacggc gtgccccgcc gctaaaatgt tacgagcacc atttacatcg gcgttcgctg
      421 tatatccaca tacctggcat ctgaatttac tttgtgacag gcggttctct ttcgctgtat
      481 gaccacagta cgcgcaacgc tggcttgtat acgctggcgg aacagcaagc acctgaccgc
      541 cacgccagag ctgcttgtac tcaagctggc ggtgcatttc ataccagccc tgatccagta
      601 tcgaacggtt taaacctgat tttgcccgga cattgcgccc cggctggctg accgtacccg
      661 ctgctgactt tgacatgttg ctgaccttca aatcctcaat gactatcatt gcgtggtttt
      721 tgctgacggc cgttgtgact ttgtgaaggt agtccctgcg gatatttgcg atacaggaat
      781 gcagtcgctg tattttgcgt ttctgcttct gccagttgtt gctgaatttg accttgcggc
      841 ttaactgtcg ctgaagtctc gccagcgtct tctggttttt ctgaaaactg tttacaggct
      901 caaagactgt gccatctgac agcgtggcga gtttagccac gccagcatcc agcccgacca
      961 ttgatgctga agggtgagcc ggagtggata cttcactttc tgtctgaata ctgatgtacc
     1021 acgtaccgca ggactggctg acagtgacat ttttcacaac acccgtgacc tgacggctat
     1081 tccggtagcg catccagccc agtttcggca gaaaaatacg gctgttttcc tgatcgagct
     1141 taacgccctg cgggtagcgg aatgcatcat tctgtccccg ctttttgaat cggggaaaag
     1201 cagcccgctt ccggaagaag tttttgtaag cccgctcaag gtctttcagt gactgttgca
     1261 atggctgtga gggagaatct ttaagccatt gcgtttcagt ggcatttttc cactcaacca
     1321 gccaggaagc cattttcccg taagggatgt atttattacc ggcctcatga ttctcattct
     1381 gacgtgccag agcacgattg aaaacgaaac gacatgcgcc tgcgaagcgc ctcatttcac
     1441 actcctgttg accacctggt cttaactgga atttaaatgc ctgtaatcgt ttcacaggtc
     1501 aaatgatact ttggtctgtg aaaaaagaaa ccgatatccg gcgtggaagg cattgtgttt
     1561 tcctgatgca tgttcacctg gtctttgtca ctagataccg acgccagatt ttttgacgcg
     1621 acagaaaaac tacgcactta cttttcaaat gtatgtgctt attttgaagc tgaactggtt
     1681 gaaatggatg gcgaaccaga tcacgtccat ttgttaatta actaacctcc caaactggcg
     1741 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
     1801 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
     1861 agtagctgcg gaggtgtgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
     1921 cctggtcagg tggaaaaccg cgccttatat ccccgccctg aaggatgggg ttttacggcg
     1981 caccggataa aaagcgccat ctaacgatgg cgcgtctgga actacttttt gttaacgtca
     2041 aacatggtgg catcggtcgc catgtcatca ataacttgtt taatattttc gaaagccatt
     2101 ggcgtacttt cgttattcag gtctttacct tcgccctgac gaacgacttt gataaccggt
     2161 ttattagtcg ctgcatcaat cagctcacct tcaaaataga ggcgagtatc catggtacgg
     2221 tggcctgtag ccatttgcgt ccccgcaacc actaatgcaa caggaaccac ttcatagaat
     2281 tgcagccctt ctttgctggt atctacaccg gtaatggccc cacggaaaat cagactacgc
     2341 ggcccagcgg tggtaaccag tggtttacgc tgcgctatcg cttctttcat ttcggtgttg
     2401 gtatagttca aaattttatc cagaactttc tgccctacct aggtagaagg ttttggcacc
     2461 ggataataag tgattgggtt ccagacgatg ctgtcatatt tgctttgatc aaaactcggg
     2521 tctacccaac gtaaaacagg tttacctgtt gctgaggttg tttcttttaa atcagagtaa
     2581 ttgtttaaaa aaccagaata tttatctggc tgggtgattt tagaggcaca accagacaga
     2641 gccaacaagc cgcataaagc tgcaactttc gcaaatgatg tggtacgcat gaatagtttc
     2701 ctg
//
LOCUS       DAZOZY010000324         2615 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZOZY010000324 DAZOZY010000000
VERSION     DAZOZY010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.327"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            96..605
                     /gene="eco"
                     /locus_tag="ACHURQ_004220"
     CDS             96..605
                     /gene="eco"
                     /locus_tag="ACHURQ_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR0825835.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(914..2560)
                     /gene="mqo"
                     /locus_tag="ACHURQ_004221"
     CDS             complement(914..2560)
                     /gene="mqo"
                     /locus_tag="ACHURQ_004221"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR0825836.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          615 a          726 c          653 g          621 t
ORIGIN      
        1 aattgtctga atgttcttta agttattcat aaagcaaatt aataaatctg atgaatatgt
       61 taaccttcag cgacatcatc ggtgaaaacc tataaatgaa gaaggaaagc aaaaaaatga
      121 agaccattct acctgcagta ttgtttgccg ctttcgccac cacttccgcc tgggcggcag
      181 aaagcgtcca gccactggaa aaaatcgcgc cttatccaca agctgaaaaa gggatgaagc
      241 gtcaggtgat tcagttaacc ccgcaagaag atgaatctac cctgaaagta gaactgttaa
      301 ccggtcagac gctggaagtc gattgcaatt tgcatcgtct cggcgggaag ctggaaaaca
      361 aaacgctgga aggctggggc tatgattatt atgtctttga taaagttagc tcgccggttt
      421 ccacgatgat ggcctgcccg gatggcaaga aagagaagaa atttgtcacc gcgtatctgg
      481 gcgatgctgg aatgctgcgt tacaacagca agctgccgat cgtggtgtat acgccagaca
      541 atgtagatgt gaagtaccgc gtctggaagg cggaagagaa aattgacaac gcggtagttc
      601 gctaaactgc tgtgaagtgc gtcaccccgt aggtcggata aggcggtcac gccgcatccg
      661 acatctaacg cccaagccgg ttgcctgatg cgacgctggc gcgtcttatc aggcctacac
      721 cgctgtgaag tgctccaccc cgtaggttgg ataaggcggt tacgccgcat ccgacatcta
      781 acgcccgagc cggttgcctg atgcgacgct gacgcgtctt atcaggccta caccgctgtg
      841 aagtgctcca ccccgtaggt cggataaggc ggttacgcca catccggcat accatgccgg
      901 atgtggcaca tcattacaac gcaatatccg ccacttcttt ttgaacgggt tgcggtttca
      961 actgcggttt cggcgtacta tctgctgcct gcggcttgtc gtatttcagg cccagcactt
     1021 cgctggtgta ctgcaactcg cgttctgttg ccgctacatc accgttcagc ttgcgtccat
     1081 aagatggaac gatcgctttc aacgtagcct gccattgcgg gctggaaacg cgatcgccaa
     1141 atactttttc cagcagatcc aacataatcg gcgcagcggt tgacgcccct ggcgatgccc
     1201 ccagcagcgc ggcaatggtc ccttgctggt cactgaccac ttcagtaccc agacgcagta
     1261 caccaccttt atcggcatca cgcttgataa tctgcacacg ctgccccgct tgccacaaac
     1321 gccagtcctc ttttttcgcc tgcggatagt actctttcaa cgcttcaaaa cgatcctctt
     1381 cactcaacat cacctgactc accaagtatt tcaccagatc gaaattatcc agcccgacgt
     1441 gcatcatcgg catcacgtta gaggtggtgg tggaactcat cagatcccac aatgaaccgt
     1501 ttttgaggaa tttggttgag aaggtggcaa atggcccaaa cagcactacg cgtttaccgt
     1561 ccagaacgcg ggtatcgata tgcggaaccg acatcggtgg tgcgccaacg gatgctttac
     1621 cgtaaacctt cgccagatgg tgattaacca cgtccgggtt ttccgaaaca aggaactgtc
     1681 cgcccaccgg gaaaccggcg tagtctttcg cttccggaat ccccgattcc tgtaacagct
     1741 tcagcgccgc accgcccgcg ccgataaaga caaatttggc acgaatgttc tgtgcagtgc
     1801 catttttcag atcggcaacg gtaacggtcc aggtgttatc gtcattacgc tttagggcgc
     1861 ggacttcgct gctgagttgc agcgagaagt tagatttctt ctgcaaggaa gcaattaact
     1921 ggcgggtgat ctcgccgtag ttcacatcgg taccaatttc cgtacgcgtg gctgccactt
     1981 tctgttgcgg atcgcgccct tccatcacta acggtgccca ctctttgatc tgcgcgtgat
     2041 cttcagagta acgcataccg cgaaacagcg agctttgttg caacgcggcg taacgggcgc
     2101 gcaggaaatt aacgttatcc tcgccccaga caaagctcat atgcggaacg gtattgataa
     2161 atgaacgcgg agtacgcagc acgccgcgct cgacctggtg cgcccagaac tggcgggaaa
     2221 tctgaaatgc ttcgttaatg gcgactgctt tttcaatact gatactgcca tcggcgtttt
     2281 gcggggtgta gttcagttcc atcagtgcag aatgcccggt tccggcgttg ttccagccgt
     2341 tcgaactctc ctgcgcgaca ccctccaggc gctccaccat ggtcatcgac cattcaggct
     2401 ccagctcgcg taaataggtc cccaacgtgg cgctcataat gccgccgcca atcaacagta
     2461 catcagtttc ctgctcctcg gacgctttcg cttttgccgc catcgaaacg gcattaagcc
     2521 ccacggccat cgagaagagc atggcagtca cttttttcat cttgttaatg ccttactttt
     2581 agtcgcttta ttgcaggtga gatttgcgcg gcatc
//
LOCUS       DAZOZY010000325         2580 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZOZY010000325 DAZOZY010000000
VERSION     DAZOZY010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.328"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            113..445
                     /locus_tag="ACHURQ_004222"
     CDS             113..445
                     /locus_tag="ACHURQ_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR0825837.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            592..1953
                     /gene="yegQ"
                     /locus_tag="ACHURQ_004223"
     CDS             592..1953
                     /gene="yegQ"
                     /locus_tag="ACHURQ_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR0825838.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2283..>2580)
                     /locus_tag="ACHURQ_004224"
     CDS             complement(2283..>2580)
                     /locus_tag="ACHURQ_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825839.1"
                     /translation="SLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDNQYYTEAE
                     ASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT          677 a          669 c          617 g          617 t
ORIGIN      
        1 actgacatta tctctccgcc ctggcaggca tcagaaaatc gctcatactt taatcggtaa
       61 acagcacctt tagatgctgt tttcgatacg caatttcaat caaggagtca ttatggctgg
      121 ttggtttgaa ctcagtaaga gcagtgataa tcagttccgg tttgtgctaa aagcgggcaa
      181 tggtgagact atcctcacca gcgagctgta tacatcaaaa gcctctgcgg aaaagggcat
      241 cgcgtcggtg cgtagcaaca gcccgcaaga agaacgctat gagaaaaaaa cggcaagtaa
      301 cggcaaattc tatttcaatc tgaaagccgc caatcatcaa attatcggct ccagccagat
      361 gtacgccacc gcgcaatctc gtgaaaccgg aattgcctcc gttaaagcca atggcacaag
      421 ccagacggtg aaagacaata cgtaattacg atgccgggcg tgggttgtcg gcgtccggcg
      481 ctttaccaga tccccccaca gcgctacaat gcccgccctt aaagtggggg cactccccta
      541 accgcttcat caggtgaagc ggagctgacc tgtcatcaga acgagagaat tatgtttaaa
      601 ccggaactcc tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc
      661 gcagatgctg tttatgcggg ccagccgcgt tactccctgc gtgtgcgcaa caacgaattc
      721 aaccacgaaa atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat
      781 gtcgtggtca acattgcacc gcacaacgcc aagctgaaaa cctttatccg tgacctgaaa
      841 ccggtggtgg aaatggggcc ggatgcgctg attatgtccg atccagggct gattatgctg
      901 gtgcgtgagc acttccctga aatgccgatc cacctctcgg tgcaggctaa cgccgtaaac
      961 tgggcgacgg tgaaattctg gcagcaaatg ggcctgaccc gcgtgatcct ctctcgcgag
     1021 ctgtcgctgg aagagattga agagatccgc aatcaggtgc cggatatgga gattgagatc
     1081 ttcgttcacg gtgcgctgtg catggcctac tccggtcgct gcctgctctc tggctatatc
     1141 aacaagcgcg atccgaacca gggtacctgc accaacgcct gccgctggga gtacaacgtc
     1201 caggaaggga aagaagatga cgttggcaac atcgtacaca agtacgagcc gattccggtg
     1261 caaaatgttg agccgacgct aggtatcggc gcgccaaccg acaaagtgtt tatgatcgaa
     1321 gaagcccagc gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc
     1381 atgaactcga aagatctgcg cgccatcgcc catgtagaac gcctgaccaa aatgggcgtg
     1441 cattcgctga aaatcgaagg ccgtactaaa tctttctact attgcgcacg caccgcacag
     1501 gtttatcgta aagctatcga tgatgccgct gcgggcaaac cgttcgatac cagcctgctg
     1561 gaaactctgg aaggtctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact
     1621 cacgacgatt atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt
     1681 ggtgagttta ccggtgagcg caagggggac ctcgcagcgg tagcggtgaa aaataaattc
     1741 tccgttggcg acagccttga gctgatgacg ccgcaaggca acattaactt tacccttgag
     1801 cacatggaaa acgccaaagg tgaagcgatg ccggtcgcac caggcgatgg ttatactgtg
     1861 tggctcccgg tcccgcagga tcttgagcta aattacgcgc tgctgatgcg taatttctcc
     1921 ggggaaacca cgcgtaaccc acacggtaag tgattaattt cgattatttt tcccggatgg
     1981 aaaattctta gaaaccgatc acatacagct gcatttatta aggttatcat ccgtttcgct
     2041 gaaaaacata acccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat
     2101 ctcccttaca cgggcttatt ttttatgcat aagccctatc cctggtcacc gtcttccatt
     2161 gaccacatcg atagaatctc ccttcatagc acgatgcctt tcacgtaacg gcatcgtgct
     2221 cgcacaggtt ccggctaagc acaaccagaa cgcgcatgtt tgatgcttac caaaaaatat
     2281 tctcactctc cacatttgaa tgtcagacga gcgacgccat gtaatcctgc accttctgtc
     2341 ttcaggtcaa ctatctgcat ttttttgccc tgagtaacac agaaatgggc tgcatcattt
     2401 tttactatat tttctgcacc agatattctg cccctagcta aagaagcttc ggcttcggtg
     2461 tagtattggt tatcgagttt acgctgaata ttacttttat atgcaagacc aaatttaccg
     2521 atacttgttt catcattatg cacagcacaa ccagatataa gaaaaatact aattaatgat
//
LOCUS       DAZOZY010000326         2561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZOZY010000326 DAZOZY010000000
VERSION     DAZOZY010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.329"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..803)
                     /locus_tag="ACHURQ_004225"
                     /pseudo
     CDS             complement(1..803)
                     /locus_tag="ACHURQ_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(856..1452)
                     /locus_tag="ACHURQ_004226"
     CDS             complement(856..1452)
                     /locus_tag="ACHURQ_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR0825840.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHURQ_004227"
     CDS             complement(1479..2081)
                     /gene="yadL"
                     /locus_tag="ACHURQ_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR0825841.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2096..>2561)
                     /locus_tag="ACHURQ_004228"
     CDS             complement(2096..>2561)
                     /locus_tag="ACHURQ_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0825842.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          712 a          552 c          513 g          784 t
ORIGIN      
        1 aagcatagcg gtaaaacaag tgggattagt caacttgaca ttgagcagat cgaaatcgat
       61 tttgataaaa taactatgct catttatacc ttggcctgcg ccataggctt taaatgaata
      121 cagataattt gatgagctgg ataatttgat ctgttgagtt actgttggat caaagtttgt
      181 atctgtatag aattcaattg taagggtctg ataaatacca cctacagccc agtatttacg
      241 ggtgctattc gcattattgc aactccaata taacacgtct tcgcttggat tataccacga
      301 aaaatattgt tctgcagagt caccaatgta tatcccaggt gaactaacgt tagttacggt
      361 accgtaagct gaccacatgt ttgaaattaa taatgtgtaa tacaaaccag gaactgatgt
      421 attaaataat ttatgggagc catacatttt acctgagtca accattgcat tttcaatacg
      481 cattgcgcta ccacttgtgc tatcacttct gttgcaataa agaaaaccac caccagaagc
      541 ggtatctaac tgactccact gtaattttac acctgtaggt ccaagctgcg tcggtttgta
      601 gaaaactaaa ttatttgtac ttgttgcata tctgaatatg acagtagcat cgccattcgg
      661 ccccgtttct ctccattttt gatctgacgg accaacgccg taactattcc caacaatata
      721 tcctacgtta tcatcagtgc cagcactcgc ggtataacaa ctcaagcaca gaattaaaaa
      781 aaatgtctga agaatttctt cattatcact ccaccatcta ctgctgtaac agtcgcagtt
      841 atttgttttg atctattatt cgtagtacac atcaactaat atatgactca ttacttggcc
      901 agatatgaca tccagcgcgg gatcgatttt ttgcattctg gcataaaatt tccatgaaga
      961 attatgcagg gcagaagtca ttactggata cacggaacga gaatttcctg ctgaactaat
     1021 aacattgaat atattggtcg ggtcctgaat ggaaaagaga acgacaccga catttttagc
     1081 cccggttgcg acttcattgg caaatatttg ccttgaacct gcagccatga caccgctaag
     1141 ggctctaaaa tcaatatgta actgattaag cacatcgcca gttccctgta atgcacagtc
     1201 tgatactgtg acagtgaact cttttccacc tggttgatag ctttcaggag taacattatc
     1261 tgaaaaatat cccaccccaa cgacgccaag atcaatatta ccctggttac tgattccgct
     1321 tttgcaagtg ctgtttttta cattggcaac aaggtcaata tcctgcccag cgatggctgg
     1381 ataggtaatg ggaaactgta gtgacagaaa cagaattatt ctcttcatca gcttacgttg
     1441 agtgggatgc atatatagtt cctggatctg tcagtctgtt agttataggt tactgtaaat
     1501 gtggcaggtg cagaaaattt acccgcagtg acattgctta cggttttatt tttttcgacg
     1561 accagtcgag cactcatcgg ataataaaca accgcgttgg ctgcattgga gatagtgacc
     1621 tcttgagatg ctggtgtcac acaactaaat tgtgttgcgc tccctgttgc cggagtttcg
     1681 gtgctccaga cttcaacggc gacggcactt gctttatctg cggctgtgga tccatttgcg
     1741 agccccgcac cgtcattagc agtaccttcg catgtggctc gcttggttaa ttttatttgt
     1801 gcttttttat tggggatacc cgcacagtct ttgaatttga gtttgaatgt tttaactttg
     1861 gtcttggcat tgatttcaga aatatataca tcgccaaagt caacaacaga aatgtcctga
     1921 tcagagtcat ttactaatgt agcggtacaa gtacctattt ctacggtagt aatcacggtc
     1981 agcccaatgc tgtctgttgc cgcataagag ggtacgctga acaatgaggc agccaaaacg
     2041 acactgctgg ataatccata acgtaaattt ttaaatgtca tcttaattat ccttcttatt
     2101 gatagatgaa attaaacgtt gccagtgcac gaaaattccc cggtacgcct tgccctgcat
     2161 ctgtttcacg tagcgcaacg gtcatttcaa gacccttatc gggctgcatc tcgtctgtgc
     2221 tccagcgtat cttttccgca ctgttaggtt taaggaaagt ggcatcatca gtagtccgtt
     2281 ttttgaaacc cataccgata ttacttgtcg tcgaagatga atcaccagac tgcggtataa
     2341 taagcttagg tgagcttgat gatgcatttc cggtcagagt ggtatcaatc caactgatgc
     2401 cactgctgca cccactagca accagtttaa aatcagcctg agattctgtc gttttattcg
     2461 cgatcttatc cagacccatc ttagggattc tcaatgtgta gttattattg ccatcattcg
     2521 tgacgttatt accagtaagt gtgatgttac aggttgtcgc t
//
LOCUS       DAZOZY010000327         2556 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZOZY010000327 DAZOZY010000000
VERSION     DAZOZY010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.330"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHURQ_004229"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHURQ_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR0825843.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHURQ_004230"
     CDS             complement(390..1178)
                     /locus_tag="ACHURQ_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR0825844.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHURQ_004231"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHURQ_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR0825845.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHURQ_004232"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHURQ_004232"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR0825846.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHURQ_004233"
     CDS             complement(2092..>2556)
                     /gene="pagP"
                     /locus_tag="ACHURQ_004233"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR0825847.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          664 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgt
//
LOCUS       DAZOZY010000328         2546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZOZY010000328 DAZOZY010000000
VERSION     DAZOZY010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.331"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..82
                     /locus_tag="ACHURQ_004234"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHURQ_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            829..1674
                     /locus_tag="ACHURQ_004235"
     CDS             829..1674
                     /locus_tag="ACHURQ_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0825848.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1685..1873
                     /gene="dicB"
                     /locus_tag="ACHURQ_004236"
                     /gene_synonym="ftsT"
     CDS             1685..1873
                     /gene="dicB"
                     /locus_tag="ACHURQ_004236"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR0825849.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1861..2061
                     /locus_tag="ACHURQ_004237"
     CDS             1861..2061
                     /locus_tag="ACHURQ_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR0825850.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2146..2421
                     /locus_tag="ACHURQ_004238"
     CDS             2146..2421
                     /locus_tag="ACHURQ_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825851.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          744 a          556 c          599 g          647 t
ORIGIN      
        1 agatgcaaaa cgcctccagc aactggagcc aaatgcaggc actggggcac gcatctggct
       61 ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct acaaagcaga
      121 aggccgtaca ggatattcac cgggtcttgg cggggtataa ataccatgcg cattgactga
      181 attcgcaaac aaaaacagac gcgcgatacc tggatagtcg gtctgcatag tcagatatct
      241 ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg tccgggagtt
      301 gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag agcatatatt
      361 tatgaagccc ttcgtaatcc tctggctcag cattatatag ttctactctg gcgagatacg
      421 ttggcatatt catttcctta ctggttgtgt gagaacttca gtaaagatac caccaaagcc
      481 cggaggtggt gaaataaaac cgggcacaac acgaaggcgc atttccggta tccataaaga
      541 gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc tccggttgta aataacgaca
      601 ttgctgtgtg aagtaccagt tggcggcatc ggtttaattg ctggctgatg tccgcccttt
      661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca gttaaaacag
      721 taaggaaaat cccttattcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
      781 aggcaccgca tcaacaaagt tcacttcggt gatgaaagga aagagaaaat gttgaatgta
      841 gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa aacgggtgcc
      901 gggattgcta ccccgaagtc attcacggcg cataaccgcg ctcaggcggt ttttttatgc
      961 gtaatgcaca gccacattca aattatggtg gggcgtgcgg ggcagtcgca agactggccg
     1021 ggttccgtga tgaccggttg tagcaaccct gtacgtctca ccacccatga gattgctacc
     1081 tccggtggtg agttaatcaa attcatcgcg gaggctgcca tcatggctac taccctttct
     1141 cacccttgtg taaccgtaga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
     1201 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatattga atgctcagat
     1261 aaatttactg ccctcaattt tgaagcgagc gaatacaccg actcaatcgg gcgcaaactc
     1321 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg ttatgggctt cactggcaaa
     1381 aaagccgctg cattcaaaga agcctacatt gctgagttcg accgcatgga ggccgaactg
     1441 cgccagaata acaccccacc tgctgacaag atgattccgg gtgatggtcg cactctggtt
     1501 gttcacttcg acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg
     1561 gtctgtaccc tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac
     1621 cggggcgcaa ttaaaaatat gactgtggaa caattactca aaattcattg ttgaggacgc
     1681 aataatggag acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt
     1741 cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac acgaacggga
     1801 gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg
     1861 atgtgcacaa tgaatacagt atttgcactc gttctgacgg tttttcttgt ttccggtgaa
     1921 ccggttgaca tggttactgg cgtatacagc tcaatgaaag aatgtatgac tgccgcagcg
     1981 aaacagaaaa ttcccggtga ctgttatccg gtcgataaag ttattcacca tgataataac
     2041 gaaatcccgg caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg
     2101 ccagcaatgg caggggttcg ctcaacctta attaaggaga aaaacatgat taccagttat
     2161 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
     2221 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
     2281 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
     2341 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
     2401 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgagtgaag
     2461 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
     2521 atgagcaccg ataaacaagt ttaccc
//
LOCUS       DAZOZY010000329         2530 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZOZY010000329 DAZOZY010000000
VERSION     DAZOZY010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.332"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHURQ_004239"
     CDS             <1..656
                     /locus_tag="ACHURQ_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR0825852.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHURQ_004240"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHURQ_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR0825853.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHURQ_004241"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHURQ_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR0825854.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHURQ_004242"
     CDS             2258..2494
                     /locus_tag="ACHURQ_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR0825855.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT          655 a          559 c          645 g          671 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata
//
LOCUS       DAZOZY010000330         2473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZOZY010000330 DAZOZY010000000
VERSION     DAZOZY010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.333"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            81..1034
                     /locus_tag="ACHURQ_004243"
     CDS             81..1034
                     /locus_tag="ACHURQ_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR0825856.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1075..2070)
                     /gene="pgl"
                     /locus_tag="ACHURQ_004244"
     CDS             complement(1075..2070)
                     /gene="pgl"
                     /locus_tag="ACHURQ_004244"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0825857.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2366..2461)
                     /locus_tag="ACHURQ_004245"
                     /pseudo
     CDS             complement(2366..2461)
                     /locus_tag="ACHURQ_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          674 a          618 c          566 g          615 t
ORIGIN      
        1 taatttaagt tggctaaatg cagttaaaat ttttaacggc cagccaccca aaatcgtcgc
       61 ttccattttc cggagtaata atgaagcatg aattatcaag tatgaaggca tttgtcatac
      121 tggcagagtc cagttcgttt aacaatgccg ctaaattact caatattacg caacctgcat
      181 taacgcgaag aataaaaaag atggaggaag atttacatat tcaactattt gagcgtacaa
      241 ctcgcaaggt tacgttaaca aaagcaggaa aaaggttgct cccggaggcg cgggaattaa
      301 taaagaaatt cgatgaaacg ctttttaata ttcgtgatat gaatgcttat catcgtggta
      361 tggtgacgtt agcgtgtatt ccgaccgcag tattttattt tttaccgctg gcaatcggta
      421 aatttaatga gctgtatccc aatattaaag tgcggattct ggaacaaggc acgaataatt
      481 gcatggaatc ggtgctgtgt aacgaatcag actttggcat aaacatgaac aacgtcacaa
      541 attcatccat tgattttacc ccactggtca acgagccgtt cgtgctggcc tgtcggcgtg
      601 accatccatt ggccaaaaag cagcttgtag aatggcagga gttagtgggt tacaaaatga
      661 ttggcgtacg ctcctccagc ggcaaccgac tgttgataga acaacagctg gccgacaagc
      721 cctggaagct ggactggttt tacgaagtgc gtcatctttc gacgtcatta ggactggtag
      781 aggcgggact ggggatttcg gcgcttcctg gtctggcaat gccccatgcg ccctattctt
      841 cgattattgg tattccactg gtggaacccg ttatacgtcg gacattgggg attattcgcc
      901 gcaaagatgc cgtgctttct ccggcagcag aacgcttttt tgccttgctg attaatttgt
      961 ggactgacga taaagacaat ttgtggacca atatcgtcga acgccagaga catgcgttac
     1021 aggagatagg ttaatacacc ggcgcaggag agactgcgcc gggtaaatca gcggttagtg
     1081 tgcgttaacc accacccaca ttggtccctg cccgaccgca tagcggcctt tttcatgcag
     1141 tagcccctgc tcgccaacaa tttcgtatac cgagatgtgg tgagattttt gcccggcggc
     1201 aatcagatac ttgccgctgt gatcaacatt gaagccgcgc ggctgggttt ccgttggctg
     1261 gaagccttct ttactcaaca cgctgccatc ttccgaaacg ctgaaaacgg taatcagact
     1321 ggcggtacgg tcgcaggcgt ataaatgacg accatccggg gtgatatgaa tatcagccgc
     1381 ccaacaggtg tcggagaagt tttccggcat catatccagc gtctggacac attcaatatt
     1441 gccgtgtgga tctttcagtt cccagacatc cactgagctg tttaactcat tgacgcaata
     1501 cgcatattgt tcgtttggat ggaataccat atgacgcggg ccggcccctt caacggtagt
     1561 cacttccgca gggtcctgcg ccaccagatg accatcatcg ctgaccgtaa acaggcaaat
     1621 gcgatcctgc tttaatgccg gaacccacag cgtacggttg tccggtgaga tattggcgga
     1681 atggcaaccg tccagcccct cgaccacatc gacgacgccc actggcaggc catcttccag
     1741 acgcgttacg ctcacgttac ccgcattgta agaacctaca aagacaaact gcccctggtg
     1801 atcggtggaa atatgcgtcg gactacccgg cagcgcagac tctgcggcaa aggtcagtgc
     1861 gccatcgtcc ggggcaatac gatacgccag gacgcgaaac tcagggcgaa caccaacata
     1921 gagataacgt ttgtccgggc tgaccaccat cggctgcacc tgccccggca catcgacaac
     1981 ctgtgtcagc gtcagtgcgc cttcatgatt caggttccag acgtgaattt gctggctctc
     2041 agggctggcg atataaactg tttgcttcat gaacacacct caaaaatgat aaatccctta
     2101 tctttcatac acataacaac acttactgga taaaaaacag acaacaaaac acgataaaat
     2161 acaatatcgc acatcaatta aatcaataag ttagcactcc atttcgaatg gcatattact
     2221 aaaatattag tcaccgacac gaatttgaca atgccacaga tattttcgcg actattcagg
     2281 gagtatgact ctccccgctt ttgtttacct gtatcaacga ccgtaaacac tatgccataa
     2341 caacttctgg ccaacttccc attcgctcaa caatcaactc acttccggca caaataaacg
     2401 cattacgttg tagatcggta ttctggtcat ttgttgatac gcgtacatag ccaataagca
     2461 tggtaaatcc ccc
//
LOCUS       DAZOZY010000331         2452 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZOZY010000331 DAZOZY010000000
VERSION     DAZOZY010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.334"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            43..2310
                     /gene="adiA"
                     /locus_tag="ACHURQ_004246"
     CDS             43..2310
                     /gene="adiA"
                     /locus_tag="ACHURQ_004246"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR0825858.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT          613 a          674 c          603 g          562 t
ORIGIN      
        1 cttgcccgca acgaagattc cttcataacc gggtaagcaa tgatgaaagt attaattgtt
       61 gaaagcgagt ttctccatca agacacctgg gtcggtaacg ccgttgagcg tctggcagat
      121 gctttaagcc agcaaaatgt taccgtgatt aaatccacct cctttgatga tggttttgcc
      181 attctctctt caaacgaagc cattgactgc ctgatgttca gctatcaaat ggaacatccg
      241 gacgaacatc aaaacgtcag acaattgatc ggtaagcttc atgagcgcca acaaaacgtg
      301 ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg caatggatcg cgacctgctg
      361 gagcttgtcg atgaattcgc ctggattctg gaagataccg ccgactttat cgccggacgc
      421 gctgttgccg cgatgacccg ctaccgccag cagctgttgc cgccactgtt cagcgcgctg
      481 atgaaatata gcgacatcca tgaatattcc tgggcagcgc caggccacca gggcggcgtt
      541 ggttttacca aaacacccgc cggacgtttc taccatgact actatggtga aaatctgttc
      601 cgcaccgata tgggcatcga acgaacttcc ctcggttctt tgcttgacca tactggcgca
      661 tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg ccgatcgctc ctggtcggta
      721 gtcgtcggta cttccggctc taaccgcacc atcatgcagg cttgcatgac cgataacgat
      781 gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac aaggcttgat gctgacaggc
      841 gcgaaaccgg tctacatggt gccaagccgc aaccgctacg gcattatcgg gccaatctat
      901 ccgcaggaaa tgcaacctga aaccttgcag aagaaaatca gtgaaagccc gctgactaaa
      961 gacaaagccg ggcaaaaacc gtcttactgc gtggtgacca actgcaccta tgacggcgtg
     1021 tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa cctccgatcg tctgcacttt
     1081 gacgaagcct ggtacggcta tgcacgtttc aacccgatct atgccgatca ctatgccatg
     1141 cgcggcgaac ctggcgatca caacggtcct accgttttcg ccacccactc cacccacaaa
     1201 ctgctgaatg cgctttcaca ggcttcttat attcatgtac gtgaaggtcg tggggcgatt
     1261 aacttctccc gcttcaacca ggcctacatg atgcatgcca ccacctcccc gctgtatgcc
     1321 atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg gcaacagcgg cctgtcactg
     1381 acgcaggaag tgatcgacga agcggttgat ttccgtcagg cgatggcgcg gctgtataaa
     1441 gagttcaccg ctgacggtag ctggttcttc aaaccgtgga acaaagaagt cgtcaccgac
     1501 ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa ccaaactgct gaccaccgtt
     1561 caggactgct gggtaatgca tccgggcgaa agctggcacg gcttcaaaga tattccggat
     1621 aactggagta tgctcgaccc gattaaagtc agcatccttg ctccgggaat gggtgaagat
     1681 ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca ctgcctggct tggtcgccac
     1741 ggcattgtgc ctacccgcac cactgacttc caaattatgt tcctgttctc tatgggcgta
     1801 acccgtggga aatggggaac tctggttaac accctttgct ccttcaaacg ccactatgac
     1861 gccaacacac cgctggcgca ggtgatgccg gaacttgttg aacaatatcc tgacacttac
     1921 gcgaacatgg ggattcacga tctgggtgac accatgtttg cctggctgaa agaaaacaac
     1981 cctggcgcac ggttgaacga agcctattcc ggcctgtcgg tggcggaaat caccccgcgt
     2041 gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg tatccattga aaatctgcca
     2101 ggacgcatcg cggcaaactc agttatccca tatccgccag gaatcccgat gctgctgtct
     2161 ggtgaaaact tcggcgataa aaacagtccg caagtaagtt atttacgctc gctgcaatcc
     2221 tgggaccacc atttccctag atttgaacac gaaactgaag ggactgaaat tattgacggt
     2281 atttaccacg ttatgtgtgt gaaagcgtaa ccactattcc gctgaaggcg taattgttta
     2341 aataacatta cgccgcctgg ccttaggcct tttgagtatg gcaacgtttt cataaaaatt
     2401 gctgcaaaca aaaatgtcat actttttgcg cggccccacc ccgcgctttt tt
//
LOCUS       DAZOZY010000332         2410 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZOZY010000332 DAZOZY010000000
VERSION     DAZOZY010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.335"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..396
                     /locus_tag="ACHURQ_004247"
     CDS             <1..396
                     /locus_tag="ACHURQ_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825859.1"
                     /translation="EEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQRVKDARKLKPTSK
                     ENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAILILHQMLKPECLNL
                     AMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            406..906
                     /locus_tag="ACHURQ_004248"
     CDS             406..906
                     /locus_tag="ACHURQ_004248"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825860.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            982..1293
                     /locus_tag="ACHURQ_004249"
     CDS             982..1293
                     /locus_tag="ACHURQ_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825861.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            1290..1583
                     /locus_tag="ACHURQ_004250"
     CDS             1290..1583
                     /locus_tag="ACHURQ_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825862.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            1644..>2410
                     /locus_tag="ACHURQ_004251"
     CDS             1644..>2410
                     /locus_tag="ACHURQ_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR0825863.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHINLLIFGDQFDSILRDF
                     PASYPLNDKLLHGHFQSFIDSYKNIDFIISGHLPISDNRNIQVFGGANHNLLCHFNYD
                     ELPSDKALIIEQDLISHKISLLNAYEGLINYLRAYYP"
BASE COUNT          830 a          351 c          496 g          733 t
ORIGIN      
        1 gaggaaatgt tatccggtga atatgctggt ttcgtttata tctttcagtt tgaggatggt
       61 tctaattatg tcggtgctaa gcaaatctat cagcgggtta aggatgcgag aaagctaaag
      121 cccacttcaa aggaaaatgg ctggcgtgag tacctcagta gttccaatgt cgttaaaaac
      181 aaaattgaaa atggtgaaca gtactcaaaa acgattctat ggtgcttcgc aactatgcga
      241 gaggtaatga ttgtagaggc aatactgatc cttcatcaga tgcttaaacc ggaatgtttg
      301 aatctggcaa tgatgagtaa gattagatca ccaaatgcaa aggataaaaa aagactactc
      361 ggaatagtac aggaactatt ggagtacttg aattgaggta aatatatggc aagtggaata
      421 aatgggattg gtagtataag gaactatata caaacgcagg gagttagatt aaataatcaa
      481 ttccagaatg aaataattaa acgttccaag ctactatccc aaagaatgca gggtgatttg
      541 aataatagcg ttgaccgtgg ggcagtgggc tttactcaac gttccatcct tttcttctat
      601 agaaaggttg ggagaaattc agttacatcg accatcatgg ttaaagatat tcaggctaag
      661 tacctttatc aagtactggt acagccaaag gcaattgata agtttatacc tacttcatct
      721 acaagattaa ctaagcaggg caacatttca ggactgaaaa agaatctttt aagtgaacga
      781 tataaggtag tgaagggtaa gaatggtaaa gaacgattaa ttgataccgc aaagaaagat
      841 accaagaaga aaaccaaacg tgtaattggc ttacgtgaga agaaacaacg tcgccttatc
      901 tattgacttc tataaggaag ccaatgatgg tgtaagggta attatgagcg gtattcaggg
      961 aacattcagg ataagtagac aatgaatttt gagcaacatt atggtgattt aataagcaag
     1021 attaagctaa atggcatgac accagatggt aatagtatgc caattaacaa atgttttctt
     1081 gataagaaat ttcaaaaggt cgttaagaaa agtaattatc agattgatag ctatatgaac
     1141 gttatttatg agaagaagaa agatttcaaa gttggtgaag tacttgaatg ggaattacag
     1201 ggtgaaaaga tgcctgtatt tctaattgag agtaaaaagc tctttgtgaa gggtaaacat
     1261 ttttgggtat atgccgtggg gattattgaa tgattagtat attgattgag tttctgaagt
     1321 caggattgga ttttttcatt aagaagaaaa ctattgaaca ggaagtaaag aaaacgaatg
     1381 tagaagggca gattgaaacg aataaagaag agattgaaaa ggtaacattc cactggcgta
     1441 atgccctcgg ttttgtacta acccttatca ttctctataa ctggatcata gtaccagtac
     1501 ttgatgcttt tggaattatt gtgatccaag ttccattagg acaacttttg caagtgctat
     1561 tgattatggt tggtggaagc tgaaagattt ttcctttaat atgtgttttg agttaataaa
     1621 atagcaatct aaggatttga tttatgagtg atgcaattac tacagcagca ataaaaaaat
     1681 tgtatggact atccgctggg caatgtaata tttgccgaac ttctcttttt aaagagcagg
     1741 ttcacatcgg acaaatggct catgtaatag ccaaaagccc aaaaggacca aggggcgatg
     1801 acctgttagc aaatgacaat tcttatgaaa atcttatcct gctttgtgca aatgatcata
     1861 ttcttgttga tagagatcca ttcacttata cagtagaaaa attgcatcaa atcaaaagtg
     1921 aacatgaggc ttatatacag ggtgcaaccg ataatacatt attttcagat aaaaaaagac
     1981 agtcagatgt ggacttttta aacgcttact tccactttac accgtttgcg agggtacgtt
     2041 cattagttga aactttacct aattcttttc acatcaatct tcttattttt ggagatcagt
     2101 ttgatagtat tttaagggat tttccagcga gctatccttt aaatgataaa ttactccacg
     2161 gtcatttcca aagttttatt gattcgtata aaaatattga ttttatcata tctggacatt
     2221 tgccgatttc cgataataga aacattcagg tgtttggtgg tgcgaatcat aatcttcttt
     2281 gtcatttcaa ttatgatgag ctaccatccg ataaagcact gataatagaa caagatttga
     2341 tttcacataa aatatctctt cttaatgctt atgaaggtct tataaattac cttagagctt
     2401 attaccctga
//
LOCUS       DAZOZY010000333         2364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZOZY010000333 DAZOZY010000000
VERSION     DAZOZY010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.336"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            123..884
                     /gene="adiY"
                     /locus_tag="ACHURQ_004252"
     CDS             123..884
                     /gene="adiY"
                     /locus_tag="ACHURQ_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR0825864.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1021..2358
                     /gene="adiC"
                     /locus_tag="ACHURQ_004253"
     CDS             1021..2358
                     /gene="adiC"
                     /locus_tag="ACHURQ_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR0825865.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
BASE COUNT          553 a          571 c          565 g          675 t
ORIGIN      
        1 atattagtaa aactcgttat ttttatgcat gtttatattc atcatacaat tatataacca
       61 tttcccggta tcgctttgct ttagcgagaa ctggtctttt attcgcactc aggagtacat
      121 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      181 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      241 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      301 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      361 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      421 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      481 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      541 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      601 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      661 agaaaaagct gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      721 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      781 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      841 cgctgcatta tgttagtcag cacagagaac gaactgtcgc ctgattttta accttaacga
      901 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      961 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
     1021 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1081 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc cactggcggg
     1141 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1201 gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1261 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1321 aatatcgcta tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1381 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1441 attgtcggtc cgaaaatgat cacccgtgtg caggccgttg ccaccgtgct ggcgctgatc
     1501 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1561 gcatggaacg tcagcggcct gggcaccttc ggtgcgattc aaagtaccct taacgttacg
     1621 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1681 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgtg
     1741 ctctccacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcctcg
     1801 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ctggggccat tgtttccttc
     1861 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1921 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1981 ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagt
     2041 agcatttcgc caaacgcaac caaagagttc ggtctggtat cttccgtgtc ggtcatcttt
     2101 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggcca cggtcacttt
     2161 ggtaaagcac gcccggcata tctggctgtt actaccattg ccttcctcta ctgcatctgg
     2221 gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc
     2281 accgccatgt atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc cttagatgca
     2341 ccaataagca aagattaatt cccc
//
LOCUS       DAZOZY010000334         2359 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZOZY010000334 DAZOZY010000000
VERSION     DAZOZY010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.337"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            23..715
                     /locus_tag="ACHURQ_004254"
     CDS             23..715
                     /locus_tag="ACHURQ_004254"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR0825866.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(764..2263)
                     /gene="pitB"
                     /locus_tag="ACHURQ_004255"
     CDS             complement(764..2263)
                     /gene="pitB"
                     /locus_tag="ACHURQ_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR0825867.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          635 a          600 c          571 g          553 t
ORIGIN      
        1 atttaaatta aaggatatat tcatgcagtc aataacacct ccattaattg ccgttattgg
       61 tagcgatggt tcaggcaagt caacggtgtg tgaacatctt attaccgttg tcgaaaaata
      121 tggtgctgcc gaaagagttc atttaggaaa acaggccgga aatgtcggtc gtgcagtgac
      181 aaaattaccg ttgatgggaa aatccttaca taaaacaatt gaacgaaatc aggtgaaaac
      241 agcaaaaaaa ttgcctagac cagttccggc gctggtaatt acagcgtttg tcgcccgtcg
      301 cttactgcgc tttcgtcata tgcttgcctg tcgtcgtcgc gggttaattg ttctaaccga
      361 ccgttatcct caggaccaaa ttcctggcgc ttacgatggt acggtgttcc cacctaacgt
      421 tgaaggtggt cgttttgtct catggctggc aagccaggaa cgtaaagcgt ttcactggat
      481 ggcgagccat aagcctgatc tggtcatcaa actcaatgtt gaccttgaag ttgcctgtgc
      541 acgtaaaccc gaccataaac gggaatcgct ggcgaggaag attgccataa cgccacagtt
      601 aacctttggt ggtgcacaac tggttgatat cgatgccaat cagccactgg aacaggtgtt
      661 ggttgatgca gaaaaagcga ttacggattt tatgaccgcg cgtggttatc actagtcaaa
      721 aatggaaatg cccgatcgcc aggaccgggc attttcagga aggttaaatc aactgcaatg
      781 ctatccagta cagcccacca gaaagaaaaa ttgccgccgg taaagtaaac acccacgcca
      841 tcaggatgct ggttaccgtt ttacgctgta acccaccgcc gtccaccacc atcgtccctg
      901 ctaccgcaga cgagaggacg tgtgttgtgg agacaggcat cccaatataa ctggcaaggc
      961 cgatagacac tgccgccgtc atttgtgccg ccatgccttg cgcatacgtc atgccgcgct
     1021 taccaatctt ctcaccgatg gtcatcgcta cacgacgcca gccaatcatg gtgccaatgc
     1081 cgagcgccag tgctaccgcc atgatgatcc acacaggagc gtactcaatg gtgcttaaca
     1141 tatcgctgcg cagttttttc agcaggttct ggtcttcttt actgacgcct ggcagtttcg
     1201 ccagcttcgc ggaggtatca gagatgcaca gcataatgcg gcgcagctgg ctgcgctgac
     1261 tcacgcttaa cggctcgtaa ctttccatat tgcctggcag catcgtttta acgcgcgcaa
     1321 tcgcatcaaa ggtatttgcc ggatgacagt gaaactctgt tacttgcgtg ccatcagtcg
     1381 atgctgcagg caatggaggt tccatcgcaa tcaacttctg cggcagttca ggatgctgtt
     1441 gcaggtagtg ttcgaagttg gtgacggcat cgcgggtacg ggtaatttca tagccggacg
     1501 cgttcatatt gacgacgaag ccagcagggg caatccccac cagtaccagc atcaccaggc
     1561 cgatcccttt ttgtccgtcg ttcgcgccgt gcgaaaacgc cacgcccgca gcggaaacaa
     1621 tcagcgcaat acgcgtccag aatggcggtt tacgtttgcc ttttttcttt ttgcgatctt
     1681 ccggaatgcg gtgaatacgg tcacgctttt tcgtcccgct ccagtagcgt cgcagcagga
     1741 atatcaggcc tcccgcaatg accaggccga cgatagggga aacaatcagc gaggagaaaa
     1801 ttttggtcac ttcacgcagg tttaacgcat ccatcaccga tgatccggtt aacagcgcgt
     1861 tggttaaacc gatgccgata atcgcaccaa tcaaggtgtg cgaactggag gccggtaaac
     1921 cgaagaacca cgttcccagg ttccagataa tcgccgccag cagcatggaa aagaccatcg
     1981 ccaggccgtg ggttgacccc atattcagca acaaatcggt tggcaacata tggacaatgg
     2041 cataggcaac gctaagtccg cccaataaca cgccaaaaaa gttaaaaaat gccgccatca
     2101 ccacagcaag ttgtggttgc atggcacgag tataaataac ggttgccacc gcattcgccg
     2161 tgtcatgaaa accattgatt gcttcgtaga acaacacaaa tgccagagca agcaataaca
     2221 aaagccctgt gtatatatca aggccaacaa ataaatttag catatattag attacgccat
     2281 tttgaattta cgaacggacg cattatcagt gactttaacg gcatgggcaa agtgaaatat
     2341 cattttttaa cgataaaaa
//
LOCUS       DAZOZY010000335         2331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZOZY010000335 DAZOZY010000000
VERSION     DAZOZY010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.338"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHURQ_004256"
     CDS             complement(25..315)
                     /locus_tag="ACHURQ_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR0825868.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHURQ_004257"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHURQ_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR0825869.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHURQ_004258"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHURQ_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR0825870.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHURQ_004259"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHURQ_004259"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2331)
                     /locus_tag="ACHURQ_004260"
     CDS             complement(2171..>2331)
                     /locus_tag="ACHURQ_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0825871.1"
                     /translation="VKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLG
                     TIRAFDVT"
BASE COUNT          643 a          621 c          516 g          551 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc t
//
LOCUS       DAZOZY010000336         2264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZOZY010000336 DAZOZY010000000
VERSION     DAZOZY010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.339"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(126..1130)
                     /gene="argF"
                     /locus_tag="ACHURQ_004261"
     CDS             complement(126..1130)
                     /gene="argF"
                     /locus_tag="ACHURQ_004261"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR0825872.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1292..1708
                     /gene="rraB"
                     /locus_tag="ACHURQ_004262"
     CDS             1292..1708
                     /gene="rraB"
                     /locus_tag="ACHURQ_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR0825873.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1754..2257)
                     /locus_tag="ACHURQ_004263"
     CDS             complement(1754..2257)
                     /locus_tag="ACHURQ_004263"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR0825874.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          576 a          559 c          551 g          578 t
ORIGIN      
        1 aatcctccgg aagatttacg tatagcaata aaaaaattgg ggtggaataa ttgaactatc
       61 actgcataaa ataatgtgac gccgggatgt ttttgtattt cccggcatct ttatagcgat
      121 atcaattatt tactgagcgt cgcgaccatc accgctttga tggtatgcat gcggttttcc
      181 gcctgatcaa atacaatgct ggcggcagat tcgaagacct catcagtcac ttccattccg
      241 ccatgtaggc caaactcttc cgccattttt ttgccaagcg tcgtttggtc gtcatgaaac
      301 gcgggcaggc agtggaggaa tttgacctcc gggttaccgg tcaactgcat catcttgctg
      361 ttcacttgat aatcacgcag caatgcaatg cgttcagccc atttctcttt tgcttcaccc
      421 atcgacaccc atacatcggt atagataaag tcagcacctt caactccttt agctacatct
      481 tccgtcagcg taatattccc gccattttgt tgtgccaggg cgcggcattc cgtaaccagc
      541 gcagcttccg gccagcatgc ctgtggcgcg accagacgca aatccagacc ggtaagcgct
      601 gcagcttcga gcatcgaatt gcccatgttg ttacgtgcgt cacctgcata gactagcgtc
      661 atttcgttga acgttttgcc gggcaaatgc tcctgcatgg tgaggagatc cgccagcagc
      721 tgcgtggggt gaaactcatt ggtcaggccg ttccataccg gcacgccagc gtattccgcc
      781 agtgtttcga caatctcctg accatagccg cgatactgaa taccgtcata catgcgacca
      841 agcacgcggg cagtgtcttt aatcgactct ttatgaccaa tttggctgcc gcttgggccg
      901 agataagtaa cacgagcccc ctggtcatat gcggcaactt cgaaagagca tcgggtacgg
      961 gtcgagtctt tttcgaagat gagcgcgatg tttttaccag tgagtttggc ttcttcttta
     1021 ccgcttttct tatcggcttt cagcttcgcg gctaactgca gcaggctgtt gagttcagct
     1081 ggcgtgaaat cgagtaattt caggaaatgc ttatgataaa acccggacat aggtccctcc
     1141 tgtggctaac gccttaatga attaaaattc aatttatatg gatgattatt catttgcaag
     1201 tctaaagcat aaatctttat cacaaaggtg gaggcaatgt cagtggtgtg tgacaataag
     1261 agtatcggca ggacattaag aggaatgagc catggcaaac ccggaacaac tggaagaaca
     1321 gcgtgaagaa acacgtttga ttattgaaga attactggaa gatggcagcg atccggacgc
     1381 gttgtacacc atcgaacacc atctttccgc agacgatctg gaaactctgg aaaaagcggc
     1441 agttgaagcg tttaaactcg gttacgaagt gaccgatcca gaagagctgg aagttgaaga
     1501 tggtgatatc gtgatttgct gcgacatcct cagcgagtgc gcgttgaatg ccgatctgat
     1561 cgatgcccag gttgaacaac tgatgacgct ggcggagaaa tttgacgttg agtacgacgg
     1621 ttggggcact tactttgaag atcctaacgg cgaagatggc gacgatgaag attttgtcga
     1681 tgaagacgat gacggggttc gccactaatt aattgattaa aatgcccgat agcacatact
     1741 agcgggcatt tttttagagt tcgcgtagca tccgcacttc acaatcgaca tggcccgtgc
     1801 agccaagcgc atagtcgata tgctcaaagc ccaaatgctc ataaagcgca atggcttcct
     1861 ttaaaaaagc ggtcgtttcc agatagcagc gtttgaagcc catctctcgt gcctgctcca
     1921 tcgccattaa ggccagtttt ttcgctagcc ctttaccgcg aatagccgga agaaaataca
     1981 tcttttgcag ttcgcaaatg tccgactcac tcccggttaa tggcgcaatc ccaccgccgc
     2041 cgaccacttc accctcgtac tcaaccaccc aatatgcatg gccaggctga ctatacactt
     2101 gatacagctc gtcaagattc ggatcggcga cggtgtagcc tttatcagcg gtaagaccgt
     2161 attcggcgga tacctgacga atgacgcggg cgatagcagg attatccagc agcgtcaggc
     2221 gacgcattac aggtgattgc ggcgcaatgt tattcatagt ttaa
//
LOCUS       DAZOZY010000337         2252 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZOZY010000337 DAZOZY010000000
VERSION     DAZOZY010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.340"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(395..529)
                     /locus_tag="ACHURQ_004264"
                     /pseudo
     CDS             complement(395..529)
                     /locus_tag="ACHURQ_004264"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(542..1363)
                     /locus_tag="ACHURQ_004265"
     CDS             complement(542..1363)
                     /locus_tag="ACHURQ_004265"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR0825875.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNEGILLNGKTIRKLMKELGISSLIRRKKYRAYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1352..2252
                     /locus_tag="ACHURQ_004266"
                     /pseudo
     CDS             1352..2252
                     /locus_tag="ACHURQ_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1632..1748
                     /locus_tag="ACHURQ_004266"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          568 a          505 c          539 g          640 t
ORIGIN      
        1 cctgatctct gtcccggtat tttagcccga ggtatattct gaaatatcat tcatcatgac
       61 cggcgtttat acaaacgccg gtcattcgct aacagcttca ccgttccttc ccccatccag
      121 gttccgcatg agcgtgttgt aatgttcata agcgtattat caggattgtc tggaaaattc
      181 ccgatacttt ggactattac tcctgaacaa ttcgaaattc agcagattaa aggtaagcgt
      241 taacggaggt aagcgttaac ggaggtaagc gttaacggag gtaagcgtta acggaggtaa
      301 gcgtaaaccg actgccgtat gtagccatta agccagtatt ggtaacgtag gtgcccacct
      361 tttcaaatga tctgcgcccc aaatcttgga catcctatga aaaatgttta agtcgatatt
      421 gaaccggact catgccatcg agtttctctt taatgcgatg gtgattgtag tattcaatgt
      481 aatcaattat atcttgcttt agatgatgta acttattgta tttatttagg tagtaacatt
      541 cttattttaa agtactaaaa aagctttcca ttgctgcatt atctaagcag ttaccacgtc
      601 gggacatgct ttgtgttatg ccattatcac tcaaccaacg ttgccaacga ggcatctgat
      661 attgccaccc ctgatcacta tgcaacaagg gcttatcgtc tttattgagt tgttctaccg
      721 catctaatag catgctcttt accattgatg ctaatgggcg ctcagtaagg ttatatgaga
      781 ctatttctcc attatacagg tccatgattg gtgataaata gagcttcctg ccatcgacct
      841 taaactctgt gacatctgtt acccactttt gatttgggcg gtcagcataa aattgtctct
      901 tcagtaaatt attacaagtt cgcccctgtt ctccacgata ggcacggtac ttcttgcgcc
      961 tgataagtga gctgataccc agttccttca ttaactttct gatggtttta ccattaagta
     1021 aaatgccctc gttacggcag gcaagtctga tgcgtcgata accataacgc cctttatgct
     1081 gatggtatat tctcaatatt acttgtttaa caagttgata tggatctggc gcattaaggc
     1141 gtttcacgtg atagtaaaat gtactacgtg gcaaatccgc gataaccagt aattgtgata
     1201 atggatgaca ttgccttaac tcattaataa tgattacttt ttcttttgtt ccaatcgtaa
     1261 tttttcctga gctaaggctt tcagcttttt tagataatga accgccccgg gaatcctgga
     1321 gactaaactc cctgagaaag aggtaaacag gatgactaaa aatactcgtt ttcccccgaa
     1381 gtccgtcaac gggcagtccg tatggttctg gaaagtcagg gcgaatatga ctcacaatgg
     1441 gcggtaattt gttccattac tccaaagatt ggctgtacac cggagactct gcgtgtctgg
     1501 gttcgccagc atgagcggga taccaggggc ggtgatggag ggctcaccac cgctgaacgt
     1561 cagcgtctga aagagctgga gcgtgagaat cgtgaactgc gctgcagtaa cgatatcctt
     1621 cgccaggctt ccgcttattt tgcgaaggcg gagttcgacc gcctctggaa aaaataatgc
     1681 cactgctgga taagctgcgt gggcagtacg gggtcggacc ggtatgcagt gaactgcata
     1741 ttgccccgtc aacgtattac cactgttagc aacaggacat catcctgata aacgcagtgc
     1801 ccgtgcgcag cacgatgact ggctgaagag agagatacag cgcgtatacg atgaaaatca
     1861 tcaggtgtac ggtgtgcgta aagtctggcg ccagttgtta cgggaaggaa tcagggtggc
     1921 cagatgtaca gtggcgcgcc tcatggcggt tatgggactt gtcggtgttc tccggggtaa
     1981 aaaggtccgc actaccgtca gccggaaaac cgttgccgca ggtgaccgcg taaaccgtca
     2041 gttcgtggca gaacgtcctg accagctgtg ggtggctgat tttacttacg tcagcacatg
     2101 gcagggcttc gtctatgtgg cgttcatcat tgatgtgttt gccggataca tcgtgtggtg
     2161 acgagtctca tcgtctatgg aaacgacatt cgtgctggat gcactggagc aggcgttgtg
     2221 ggcccgtcgt ccgtctggca ccatccatta ca
//
LOCUS       DAZOZY010000338         2239 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZOZY010000338 DAZOZY010000000
VERSION     DAZOZY010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.341"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            598..945
                     /locus_tag="ACHURQ_004267"
     CDS             598..945
                     /locus_tag="ACHURQ_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR0825876.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(965..1555)
                     /locus_tag="ACHURQ_004268"
     CDS             complement(965..1555)
                     /locus_tag="ACHURQ_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825877.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1552..1812)
                     /locus_tag="ACHURQ_004269"
     CDS             complement(1552..1812)
                     /locus_tag="ACHURQ_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825878.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            1910..2214
                     /locus_tag="ACHURQ_004270"
                     /pseudo
     CDS             1910..2214
                     /locus_tag="ACHURQ_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          540 a          592 c          560 g          547 t
ORIGIN      
        1 cgtcatacag acctcgtgat cgagttaata agactatgat agaactacaa actaacggag
       61 tagaacggca acttttagat aattcttgtg gcataagttc tggggtattt aaataaaata
      121 taagacaggc tgtctatctt acagacagcc tttttataat aacagaaaga atatatatca
      181 tacttaacgg aagagataat atgaaaacag tacccgtata aacagcaagc aatcccattt
      241 ttccaggtgt atcagcaaaa aaaccgctgt tctagaaatc aggccgggta aatttcagag
      301 ctgtatcttc aatataccat tttgcaaccg gatacaaaac cattccgcag agagaaatac
      361 accagaacag taatctgtat ttaaaatcat aatcccatga catatacagc atatatcctc
      421 ccgtcaccca tccccaccac atattattaa aataatattt tctgttcatc tgccagtttt
      481 cctttctttt gcacttttgt cagtgtactg atgcatgaca acataaccac agcatgataa
      541 taataatcaa taacaataag ctgtgtcacg tttacatcat ggaacggagg tgctcctgtg
      601 acttttgagc agaaaaaagc ccgggccatt gccctgatgg acagcaaaaa gatgtggcgg
      661 agcaattatg cccctcctct tctgcgtatt ctctggcggc tgggtatccg gcttccgcca
      721 ttacccttta tgccgttctg gcaggtcacg gtgctgacgg ggggcttatg gggcatttcc
      781 tggggatgtg caatgtggtt tatctactgg gggccgtcag gaatggtggc aggtgaggcc
      841 attattataa gtatcacggg gggttttttg tccggactgc tcatggcctc tttccactgg
      901 tggcgcagga aagttaaccg actgccacca tgggatgatg tgtaaaccgt gcacaggcgt
      961 gttcttacgg agcggcactt tcatttatct gtacgcgaac ctccattcct gcatcgtgac
     1021 aggcctgcag ccactgcgcc acttccagcg gatcgccctc ccggcgtacc acccagcctt
     1081 ctttattcca taactgcaga caggtgctgc cgtcgagacg caccacaaaa tccccacggc
     1141 aggcctgata cgttatgccg ttcagctcca ccgccagatc agtggcagaa gtgccgcctg
     1201 cgggcagacc tgctgccggg accagcggca gtacgacgac ttctgccgca cgctgttctg
     1261 cccggatgcg gtcctgttct gccagcagca gaggctgtgc cacggcccgg ccggcgtccg
     1321 tcagctccac tgccagctgc aggttcggag cgcgcagggt acgcagccag ccggcatcct
     1381 ccagacgacg gcaggaggcg cgcaggtttg gcccgtagac cggggcttcc ccgctttgct
     1441 ccagcacccg ttctatgtcc cgcgtcagca cgggtcctgg ccgtttttca tcaagggcag
     1501 ccagcacaat cagcactctc cgctgcagtg gcgacggtcg gcggctctga gtcatttcca
     1561 gtcatcctcc ggccagatga gctttctgag gcgctgatgg tcggcgtcaa tgatctcctg
     1621 tctctttatg gcattcattg aggtatagcc atttatggcc agttcatgcc agaagatcag
     1681 cacggctccg gctttggctg actcgcagag ataagttacg ccgtggctgt ccagattgca
     1741 ttcccggatg cgtttttccg caaaccgggt cagttgttca tagctgagtg aaaacgtcat
     1801 ttcctgatcc atcctgcacg ttccatctga taatgttctg acaggtaaaa atcattgaac
     1861 cgccccggga atcctggaga ctaaacttcc tgagaaagag gtaaacagga tgactaaaaa
     1921 tactcgtttt tcccccgaag tccgtcaacg ggcagtccgt atggttctgg aaagtcaggg
     1981 cgaatatgac tcacaatggg cgacaatttg ttccattgct ccaaagattg gctgtacgcc
     2041 ggagactctg cgtgtccggg ttcgccagca tgagcgggat accgggggcg gtgatggagg
     2101 gctcaccacc gctgaacgtc agcgtctgaa agagctggag cgtgaaaatc gtgaactgcg
     2161 ccgcagtaac gatatccttc gccaggcttc cgcttatttt gcgaaggcgg agtttgaccg
     2221 cctctggaaa aaatgatgc
//
LOCUS       DAZOZY010000339         2190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZOZY010000339 DAZOZY010000000
VERSION     DAZOZY010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.342"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            159..668
                     /locus_tag="ACHURQ_004271"
     CDS             159..668
                     /locus_tag="ACHURQ_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR0825879.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            665..2083
                     /gene="phrB"
                     /locus_tag="ACHURQ_004272"
     CDS             665..2083
                     /gene="phrB"
                     /locus_tag="ACHURQ_004272"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR0825880.1"
                     /translation="MTTHLVWFSQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
BASE COUNT          539 a          537 c          582 g          532 t
ORIGIN      
        1 caaaaaatga atgcagctcg ggaaatagta gaataattct cactcagatg aagttgataa
       61 tatttttacc aacaaaatac ttccccgcaa aaactctgcc ccataaaatt catttaccat
      121 cgtctacact tactccttta taccgttcag gagcagtaat gaatctacaa cgatttgatg
      181 acagcaccct aatccgtatc tttgcccttc atgagttaca tcgactgaaa gaacatggct
      241 taacgcgcgg ggcgcttctc gattatcaca gccgctataa actcgtcttt ctggcgcatt
      301 ctcagccgga gtaccgcaaa cttggcccgt tcgtggctga tattcaccag tggcaaaatc
      361 tggatgacta ttacaaccag taccgccaac gcgtagttgt tttgctttct caccccgcca
      421 acccgcgcga tcacaccaat gttttgatgc acgttcaggg ttattttcgc ccgcatattg
      481 attccacaga acgccagcag ctggctgcgc ttatcgacag ttatcgccgt ggcgagcaac
      541 cacttcttgc gccgctgatg cgtatcaaac actatatggc gctttatcat gacgcctggc
      601 tttcagggca gcgttatttc gaactttggc cgcgtgtgat taacttgcgc cattcaggag
      661 ttttatgact acccatctgg tctggtttag ccaagattta cgtctgcacg ataatctcgc
      721 actggctgcc gcctgccgca attcgtctgc acgcgtgctg gcgttgtata tcgctacacc
      781 acgccagtgg gcgacgcata acatgtcgcc gcgtcaggct gaacttatca atgctcaact
      841 gaatgggcta caaatagcgc ttgcggaaaa aggtattcct ttattgttcc gtgaagtgga
      901 tgactttgtc gccagtgtcg aaatagttaa acaggtgtgc gcggaaaaca gcgttaccca
      961 cctgttttat aactatcagt atgaagtgaa tgagcgggcg cgggatgtgg aagttgaaag
     1021 agcgctgcgt aacgtggtgt gtgaaggatt tgatgacagc gtgatcctgc cgcctggcgc
     1081 ggtgatgacc ggtaatcacg agatgtacaa agtctttacg ccttttaaga atgcctggct
     1141 gaaacggctg cgggaaggga tgccggagtg cgtcgctgcg ccaaaagttc gtagtagcgg
     1201 atcgatagag ccctcgccat ccattacgct gaattatcct cgtcagtctt tcgatactgc
     1261 gcattttccg gtggaagaaa aagcggcgat tgcgcaatta cgccagtttt gccagaacgg
     1321 tgccggagaa tatgagcaac aacgagattt tccggcagtg gaaggcacca gccgtttgtc
     1381 ggccagcctg gcaacgggcg ggttatcgcc tcgccagtgc ttgcatcgct tgttggctga
     1441 acagccgcag gcgctggacg gtggggtcgg tagtgtctgg cttaatgagc tgatctggcg
     1501 cgagttttac cgtcacctga taacgtatca cccctcgttg tgtaaacatc gtccaattat
     1561 tgcctggacg gatcgtgtac agtggcagag caatcccgca catttacagg cctggcagga
     1621 aggcaaaacg ggatacccga ttgttgatgc cgctatgcgt cagcttaaca gcactggctg
     1681 gatgcataac aggctacgga tgattacagc cagttttctg gtgaaagatt tattgatcga
     1741 ctggcgcgaa ggcgagcgat atttcatgtc gcagctgatt gatggtgatt tggcagccaa
     1801 taacggtggc tggcagtggg ccgcttcaac cggaaccgat gcagcgccgt attttcgtat
     1861 tttcaacccg acaacccagg gcgagaaatt tgatctcgaa ggtgagttta tccgccagtg
     1921 gctaccggaa ctacgcaatg taccggggaa atcggtgcat gagccgtgga agtgggcgca
     1981 gaaagcaggt gtgaagctgg attatccgca accgatagtc gagcacaaag aggcgagagt
     2041 acaaacgttg gcagcgtatg aggcggcgcg gaaggggaaa taacagacgc gtcaggcaat
     2101 cgagcccaga tgccggatgc ggcgtgaacg ccttatccag cctacaaatt tgtgcaaatt
     2161 caataaattg caggaaatac gtaggcctga
//
LOCUS       DAZOZY010000340         2190 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZOZY010000340 DAZOZY010000000
VERSION     DAZOZY010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.343"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..503
                     /locus_tag="ACHURQ_004273"
     CDS             <1..503
                     /locus_tag="ACHURQ_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0825881.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSY
                     REVNRD"
     gene            496..978
                     /gene="yqeJ"
                     /locus_tag="ACHURQ_004274"
     CDS             496..978
                     /gene="yqeJ"
                     /locus_tag="ACHURQ_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR0825882.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="ACHURQ_004275"
     CDS             complement(1012..1092)
                     /locus_tag="ACHURQ_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825883.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /gene="yqeK"
                     /locus_tag="ACHURQ_004276"
                     /pseudo
     CDS             complement(1128..1552)
                     /gene="yqeK"
                     /locus_tag="ACHURQ_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1982..>2190
                     /locus_tag="ACHURQ_004277"
     CDS             1982..>2190
                     /locus_tag="ACHURQ_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825884.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEE"
BASE COUNT          806 a          358 c          364 g          662 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggaggaaaa tatctaacac atgatttgtc
     2161 aataaagata gcggatattt ctgaagagat
//
LOCUS       DAZOZY010000341         2168 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZOZY010000341 DAZOZY010000000
VERSION     DAZOZY010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.344"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(468..1019)
                     /locus_tag="ACHURQ_004278"
     CDS             complement(468..1019)
                     /locus_tag="ACHURQ_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR0825885.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1818..2114
                     /locus_tag="ACHURQ_004279"
     CDS             1818..2114
                     /locus_tag="ACHURQ_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFR0825886.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          628 a          467 c          422 g          651 t
ORIGIN      
        1 aggcttactt agtggaaaga gaggggatat gtgaccatag gggaacccct cctgttaaaa
       61 gactacttag aagtctgagg ttttagtaaa atctgtgaat atggcttctc agacgctctg
      121 taacgcattc taagcgattt taccccttta ttttaaggtg gttagtacat ccctataggt
      181 tacccctaaa aatggggtta aaactcgcta cagagctttc tatgagatta gcttctaatc
      241 gaatcaacta tgaggtttgg taggattgtg caaaatctgt acagtaactt ttcagaatct
      301 gaaaattttt ataaaaaatg actagccctc tttgaatctc atcttccccg aatcttcaaa
      361 aatatctgac agatgtctgt agctgctaag tacccgtcac accagctatg tgaccccctt
      421 ggcaaaaaag ccaaatatca gctacatcca tgtaactatt cagtaactta tttagcaagt
      481 ctgtaataag ttgctctaga gattcctaac tcttcctgaa tctgcaacct cgtcttacct
      541 tgtgaaataa gcgtctgtac agcctctgaa gaagctttta caggcctccc catatgtacc
      601 ccttcagctt tagcacgttc aataccagcc tttctacgct ctgctatcaa gcttctttca
      661 agctgtgcca acgttgacat aatgcctaac attgcttcac ccatagcgct tgataagtca
      721 aacccttctc taaggctgat aactgtcaca cctttagcct gtagcgcttt aaccgttgat
      781 aacacatcaa ttgtgtcacg tccaagacgg tctacagcta ccacaactac ggtgtcacct
      841 tctcgtgcat aagctaatag tctgctgaaa ccgtttctta aagaagcttt gacagaacca
      901 ctgacagctt catcagtgaa ccatttagca atgttgtacc ctgcctcttc aatttgcatt
      961 tgctggttag tgatggattg gtcatctgta ctcaccctgc aatatccaag tatcgccata
     1021 atgttcccct tagaagtctc aaaagttagt ctcataagtt tagctgtctc ataatccaaa
     1081 aacaaccttt tgagactact tgagggtgca ttctaccgtt acgttgaaag tgtctcaaaa
     1141 ggaagtcctt ttaagactgg ttatgaagga gctatgaggt tgattagggg ttggtacaag
     1201 cccaactaac cgaatcaact atgaagattt taggagggta actgtaacca tcactttcta
     1261 actttcacgt aggaaattgt taagtggttt gatagttaca ccatgtgacc aagcatctat
     1321 atatcgttat atcgccctgt atatagcact atatgcagtg tcgagagagg aacgactaac
     1381 acgatacagt ttaccctgaa aagagacttc acagattatt tatatatttc aataagttag
     1441 ggtaaaaagg gactataccg agactgtata gaatctgtgt agaattgcat agatacttgg
     1501 aaatctacta ttaaattgca tagggactca cctgatattg tcctgttttt aacaggtctc
     1561 ctagaaaacc gaatagactt cattcaaggg tcattgaagt cataatcatt tcagtagaaa
     1621 aaccaagtct cgattttaaa tgcaaatagc aatcatattc aataaggcaa gacgatcagg
     1681 atcagtgaaa atgtcatttt tggcgtaatt gaccttactt tatgcatggt cttaacaaag
     1741 acatcatctt tccgttgcag atcttcgccc tttcaaataa cgtactttac aactttcccg
     1801 aacaaggagt tgtgcccgtg ttgtatgtta tttacgctca agacaaagct gatagcctcg
     1861 aaaaacgcct ttccgttcgt ccggcacatt tagcacgttt acagttactg catgatgaag
     1921 gtcgtttgtt gacagctggt ccaatgccag cggtagacag taacgatcca ggggctgcgg
     1981 gatttactgg ctcaaccgtt attgctgaat ttgaatcgct ggaagcagct caggcctggg
     2041 ccgatgctga cccgtacgta gcggcaggtg tctatgaaca cgtttcagtg aaaccattta
     2101 agaaagtgtt ctgaatagag attcaatatt taagggcacg gtttttgcaa ccgtgttctt
     2161 gtttggcc
//
LOCUS       DAZOZY010000342         2127 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZOZY010000342 DAZOZY010000000
VERSION     DAZOZY010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.345"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHURQ_004280"
     CDS             <1..547
                     /locus_tag="ACHURQ_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825887.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
     gene            623..>2127
                     /locus_tag="ACHURQ_004281"
     CDS             623..>2127
                     /locus_tag="ACHURQ_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR0825888.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALW
                     QVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFG
                     RTLAFHRAAEGDVAGAVTGVMDGAGRRFHLVLTTQAQRAEEARKPHTASLSSPDSPCP
                     LSAPSFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAV
                     YDRSGMQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYG
                     ESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKG"
BASE COUNT          445 a          544 c          758 g          380 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc agggcgacat
      661 gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag gtgcgccgac
      721 gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg tgaacccggt
      781 gctgggtgcg aaggtgctgc cgggcgagac ggaccttgcg ctgccctgcc cgctgccgtt
      841 tattcttttc cgcgcctaca gcagctaccg gaccagaaca cccgcgccgg tgggggtgtt
      901 tggtcccggc tggaaagcgc ccttcgatat ccgcttacag atacgcgatg aaggcctgat
      961 actcaacgac agcggcggca ggagcattca ctttgagccg ctgtttcccg gcgagataag
     1021 ctacagccgc agcgagtcac tctggctggc gcggggcgga gtagcggcgc agcacagttc
     1081 gcagccgcta agcgcgctct ggcaggtgct gccggaagat gttcgcctga gtccgcatgt
     1141 gtacctggcg acaaacagcc ttcaggggcc gtggtggata ctcagctggc cggagcgggt
     1201 gccgggggcg gacgaggtgc tgccgccgga gccgcccgca taccgggtgc tgacgggcgt
     1261 ggtggacggc ttcgggcgga cgctggcctt tcaccgggca gcggagggtg acgtggcggg
     1321 cgcggtgacg ggggtgatgg acggcgcggg ccgccgtttt cacctggtgc tgaccacaca
     1381 ggcgcagcgg gcggaagagg cccgtaaacc gcacaccgct tcgttatctt cccctgacag
     1441 tccctgccct ctctcagctc cttccttccc cgacacgctg cccgccggta cagagtacgg
     1501 tgccgataac ggtatccggc tggaggcggt atggctgaca cacgacccgg catacccgga
     1561 tgaacagccg actgcgccgc tggcgcgcta cacgtacacg gccggtggtg aactgcgggc
     1621 ggtgtatgac cgcagcggga tgcaggtgcg cgggtttact tatgatgcgg agcacgccgg
     1681 gcggatggtg gcgcaccatt atgcggggag gccggagagc tgctaccggt atgatgatac
     1741 cggtcgggtg acggagcagg tcaacccgga ggggctggac taccgctttg agtacggaga
     1801 gagccgggta atcatcacgg acagcctgaa ccggcgggag gtgctgtaca cggaaggcga
     1861 gggtggcctg aagcgggtgg tgaagaagga gcatgccgac gggagcatca cccgcagcga
     1921 gtatgatgag gcggggaggc tgaaggcgca gacggatgcg gcgggacggc ggacggagta
     1981 ccgactgcat atggcgtctg gaaagctgac ttcagtgata ctgccggacg gcaggacggt
     2041 acggtatggc tataacagcc agcggcaggt gacgtcggtg acgtacccgg acgggcttcg
     2101 cagcagccgg gagtatgatg agaaggg
//
LOCUS       DAZOZY010000343         2115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZOZY010000343 DAZOZY010000000
VERSION     DAZOZY010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.346"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(364..1368)
                     /locus_tag="ACHURQ_004282"
                     /pseudo
     CDS             complement(364..1368)
                     /locus_tag="ACHURQ_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            1353..1730
                     /locus_tag="ACHURQ_004283"
                     /pseudo
     CDS             1353..1730
                     /locus_tag="ACHURQ_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1769..>2115)
                     /locus_tag="ACHURQ_004284"
     CDS             complement(1769..>2115)
                     /locus_tag="ACHURQ_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0825889.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT          495 a          496 c          577 g          547 t
ORIGIN      
        1 atacgtgaac tccgatattt ctgagttgtg gttatgggat agcatttaat tacaacgggg
       61 aactatcagt catggtacgt aactgataag ggtataatga taatcactgt cagttatatt
      121 tgtatgacag atgtaacaaa atgaaaaaaa aagataagca caagtgattg attggtcaga
      181 tattgatcac ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt
      241 ccggtcaatc ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg
      301 acagactttt gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg
      361 aactcagaag gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag
      421 gcctgcaaca tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc
      481 actgccgtcg ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca
      541 ggcattaaat tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct
      601 ggccggggtc agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc
      661 ggccgtcacc tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc
      721 aatactacgg gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc
      781 cgtactggca ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt
      841 gaaggcgctg ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg
      901 gaatttgggg gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag
      961 agcaataccc acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct
     1021 tttgtccacc tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc
     1081 agagacttta ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag
     1141 gcgatcaccg ggaagggccg ccacagcaga aacgcccagc tgtgcggcgg catgtgtgtt
     1201 acggaagtcc cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact
     1261 ttcgatggcg cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat
     1321 attatccgga ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc
     1381 gacgcaaaat ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga
     1441 aactaatcgg tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac
     1501 gcctggcggt caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc
     1561 aggagaaaga acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca
     1621 ggaagcactg ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata
     1681 atgcagcaga aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca
     1741 ctcattccgt gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt
     1801 gggcagcaag tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg
     1861 ctacttgtga ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt
     1921 gagctttcca tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa
     1981 gccatgctcc aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc
     2041 tcagttcgct gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat
     2101 attcacctgg cacca
//
LOCUS       DAZOZY010000344         2090 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZOZY010000344 DAZOZY010000000
VERSION     DAZOZY010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.347"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(36..974)
                     /gene="mdh"
                     /locus_tag="ACHURQ_004285"
     CDS             complement(36..974)
                     /gene="mdh"
                     /locus_tag="ACHURQ_004285"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR0825890.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            1409..1879
                     /gene="argR"
                     /locus_tag="ACHURQ_004286"
     CDS             1409..1879
                     /gene="argR"
                     /locus_tag="ACHURQ_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR0825891.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT          570 a          528 c          444 g          548 t
ORIGIN      
        1 gtctgtgctc cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc
       61 aaggcgatat ctttcttgag tgtatccagc ataccttcca gcgcgttctg ttcaaatgcg
      121 ctcagggtac cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga
      181 gagaagaaac gggcgtactg accatcgcct tcaacgtagg cacattcgac aacgccttgt
      241 tcgccctgca gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac
      301 agggttgcag acccgccacc ggctttcgct tcaaccactt cagtacccgc gttctggatg
      361 cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc
      421 agcggcagaa tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcctggc
      481 tgtttgcctt tcagttccgc aacaaaagtg ttggaacgaa tgatgtccag cgtggtaacg
      541 ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca
      601 actgtggtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca
      661 acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc
      721 atacccggtt tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc
      781 ggagtcgcat cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga
      841 tcgacagcca caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa
      901 ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg
      961 actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct
     1021 gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt
     1081 cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt
     1141 aacagtcgct tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt
     1201 cagccaaaac aacatcaatt tgataacaat taatttactt ttaaacagaa tttgcatgcc
     1261 gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt
     1321 atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg
     1381 ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt
     1441 taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc
     1501 cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac
     1561 caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc
     1621 tgaactgggt gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta
     1681 caacgatgca gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct
     1741 gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat
     1801 ctttaccacc cctgctaacg gtttcaccgt caaagacctg tacgaagcga ttttagagct
     1861 gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt
     1921 attcctctca accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttaccgc
     1981 gacatatcat tcacacaatg aatacatacg gtaaaaaaag cacattatgt aaaattcatt
     2041 atctaattga aaatactaga attaacaata aataaccgtg tttttaattc
//
LOCUS       DAZOZY010000345         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZOZY010000345 DAZOZY010000000
VERSION     DAZOZY010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.348"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHURQ_004287"
     CDS             complement(60..1025)
                     /locus_tag="ACHURQ_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR0825892.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHURQ_004288"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHURQ_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825893.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZOZY010000346         2088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZOZY010000346 DAZOZY010000000
VERSION     DAZOZY010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.349"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="ACHURQ_004289"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="ACHURQ_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR0825894.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="ACHURQ_004290"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="ACHURQ_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR0825895.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
BASE COUNT          552 a          505 c          590 g          441 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacgg
//
LOCUS       DAZOZY010000347         2079 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZOZY010000347 DAZOZY010000000
VERSION     DAZOZY010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.350"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            102..398
                     /locus_tag="ACHURQ_004291"
     CDS             102..398
                     /locus_tag="ACHURQ_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR0825896.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            404..1501
                     /locus_tag="ACHURQ_004292"
     CDS             404..1501
                     /locus_tag="ACHURQ_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR0825897.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIALDEIIELFNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNAL
                     WIELEGVEYAFPDPQTQNETQQAGYDENIPV"
     gene            1552..1740
                     /locus_tag="ACHURQ_004293"
     CDS             1552..1740
                     /locus_tag="ACHURQ_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR0825898.1"
                     /translation="MYKITATIEKEGGSPASWTRYSKKKLTKSECEKMLSGEKEAGAS
                     REQKVKLINFNYEKLQSS"
     gene            1847..>2079
                     /locus_tag="ACHURQ_004294"
     CDS             1847..>2079
                     /locus_tag="ACHURQ_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR0825899.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          663 a          452 c          493 g          471 t
ORIGIN      
        1 cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct gcaatccctg acatcgcaat
       61 gatgattgcc aacaagcttg aaaattttgg agtgaccagt gatgagcgct tacccgattt
      121 ttgatcgtat tgaagaaatg gcatgggctc gccactacca gcagatcgtt cgtgaagaaa
      181 aagaaacgga actggcggac gacctggaaa aaggtctgcc tcagcacctg tttgaatcgc
      241 tctgcatcga ccatttgcaa cgccacggtg ccaataaaca ggcaatcagc cacgcatttg
      301 atgatgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg gctgaaacca
      361 ttgcccacca ccaggttgat attgatttgg aggactaaaa tctatggcgc tttttcaacg
      421 agcaataaac acacaggcct atctaaaagc aggaattatg gggtttgctg gcgacggcaa
      481 aacctacaca gcaagcgaac tggctattgg tctggtaatg cttatgcggc aacggggaat
      541 tgaagctggt aaccgcccgg taatgtttct ggacacagaa acaggctctg actgggttaa
      601 accccgcttt gatgcggaaa atatcgaatt atttacggca aaaacccgct cgtttgttga
      661 tctgcttgaa gccattaatg aagcggaaag cagcggttcc gtaatgatca tcgactccat
      721 cagtcacttc tggaccggat tatgcgacga gtatgccaga cgccgaaacc gtaaacgagg
      781 actggaattt tcagactggg cgtggctcaa acaggagtgg cgacgcttta ccgatcgatt
      841 tgttaacagt caggcgcaca ttattatgtg cgggcgtgca ggatatgagt atgacttttt
      901 cgaaggtgat gatggcaaac gccagttggc aaaaaccggt ataaaaatga aagcagaaac
      961 agaaaccggt tatgaaccct ccatcctgat ccagatggag aagcaaatga acatagaaac
     1021 cggtcaggtg tggcgcaccg ccagaatact aaaagatcgc tccacccgta ttgatggaca
     1081 ggtattttca aacccaacat ttaaaaactt tcttcctcat atcgagtttc ttaacctggg
     1141 aggtgaacac ctcggcatcg atacctcacg ggataacagt gaattatttg ccaacgacgg
     1201 tacaccgaca tggctaaaag aaaagcgtgc aaaagaaatc gcacttgatg aaattatcga
     1261 gttgttcaac aagcaccatg ggggcaccag taacgacgcc aaacgcgcaa aagctgattt
     1321 gctggaaaaa acattcacct cccgctcatg ggaaagaatc aaaggtatgg actggccgac
     1381 aatcaaagct ggtcgtaatg cactatggat tgaactggaa ggcgttgaat atgcattccc
     1441 ggatccacaa acacaaaacg aaacgcaaca agcaggatac gatgagaata ttccggtgta
     1501 aaaccaagtg cattttggac acggtttttg aaaatattaa tggaggtgtt catgtataaa
     1561 attacagcca ctattgaaaa ggaaggtggt tctcctgcca gctggacaag atactcaaag
     1621 aaaaaactaa cgaaatcaga atgcgaaaaa atgctctcag gggaaaaaga agcaggcgct
     1681 tccagagagc agaaagtaaa actgataaat tttaattacg agaaacttca gtcctcgtga
     1741 atttcattat atccagatta gaacttcata gctgattatt aaaaatcaac cacacccgcc
     1801 agtattctgt atatttactg gcggtcatat cgtgagaggt atggcaatga atcttgtgac
     1861 actcaaaacg tggggaaaac tcagatatcc ggataaccca ccatcaatat caacgctgag
     1921 acgatgggca aggaatggaa acatttatcc tgcacctgaa ctacacggga ggagttacag
     1981 ggtggttccg gaggctttct atatcaaccc aaataaggtt gataccgata taacacacca
     2041 tcagcctaat gggcgacaag ggagagacag tccgttaat
//
LOCUS       DAZOZY010000348         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZOZY010000348 DAZOZY010000000
VERSION     DAZOZY010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.351"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHURQ_004295"
     CDS             <1..389
                     /locus_tag="ACHURQ_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825900.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHURQ_004296"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHURQ_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR0825901.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHURQ_004297"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHURQ_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR0825902.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHURQ_004298"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHURQ_004298"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHURQ_004299"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHURQ_004299"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZOZY010000349         1980 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZOZY010000349 DAZOZY010000000
VERSION     DAZOZY010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.352"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(358..1401)
                     /locus_tag="ACHURQ_004300"
     CDS             complement(358..1401)
                     /locus_tag="ACHURQ_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0825903.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            complement(1812..>1980)
                     /locus_tag="ACHURQ_004301"
     CDS             complement(1812..>1980)
                     /locus_tag="ACHURQ_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR0825904.1"
                     /translation="LHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT          576 a          404 c          425 g          575 t
ORIGIN      
        1 ccacgggaat atctgcggca gcgggcttgt aatgggttaa gtgataacag atgtctggaa
       61 atataggggc aaatccacgg ggataccagt tcaaccgaaa acgccagagg aggggattac
      121 ccgctggcag ggtaaatctg tggtttttga tgccagctat aacgtgtttg acgataaagt
      181 attacgaaag aactttaatg agttcatgga gtaattcgtt aaagtaattt acaacgctat
      241 ccttttgcaa aggaaacttt cagaaaataa ccccagggta atcacctggg gaaggaacac
      301 agtgttatta ttggataata aattattact tgttttaaag ttacactaat gcaatatcta
      361 ctgatactgg atgcttgaat gtttcatccc tgctgcggaa aagggcaata tccagggcca
      421 gatatattca ccgttcatat ttctgcattt ctgtacaatt tcctcagaaa gtgtcttgtc
      481 aattcttttc cacaggtgct cctgctgtct taaaatactt tcgcatattt cgaaatcatc
      541 ttcctgaatc agcatttcgg cgcatttaat gatttctgat cgttcatgca gcacggagga
      601 atggttatag agccagttta caaacagcaa tgcttccgga tattgtcccc ataatggtga
      661 gtatgttgca tagtaatctt ctgccgtatc cagagagccg gagaagtcat gccacacagc
      721 aagtggaaat ggatgcagat ttccgcacag catttctgcg atgtaagtgt ttgtggcaaa
      781 gccatggcga aaagctgtgg cggccttaac atgttccccg ttcagaaaat gcaggagacc
      841 gagttcatac cagtaaggtg aaaattcatc agcatgctct ttcagtacac tgaaggctcg
      901 ctcatgatcg ccggtgcgta acagctctga acccaacaac cagcgagccc cctgattatc
      961 ttcaggattg tatgccagta ttttatcagt gagcataacc gcatcctgat ggcgctgtaa
     1021 gtgcacattt gcgagaattg ctgcataaag cgcgcgcaga taaggccggt tttcgggatg
     1081 catccagatt atttcaccac agaaactttc cgggatgata cgattacctg ccgccagtcc
     1141 tttaagagag gcgttcagtg ccttacgcgg ggcattctgt tccagaaatg catatgccag
     1201 atgagcgtgt atatcaataa agtccggctc ccgttgagca aggcgtcgga gtttgttgat
     1261 gtaactcgtc tcgctgattt catcagcgtc gtatgcatca agcacttctt caaactcccc
     1321 cagcaatggg ccaaaccagg tatccgaaaa tacgaaataa cccttgttat cgtgcacttc
     1381 aaagcataat ctatcgttca tttctgagcc tgtaaaacat gtggtgatat agtctgagag
     1441 cataaaagtt gagattctgt gtatcaagtc ctgtcttggc accgaatgaa tattgcttac
     1501 gaagcccctt catgctttcc ttgagaacaa taattttatc tttatgtgaa tctcattttt
     1561 tataatgatg tcagatgatt gaaatgctaa attatatctg aatcaacttt tcctttgcta
     1621 aaggtatcga aataactgca aggctgtatt ttgtaattgc caatttaaaa atgtcaattt
     1681 gtttttaatt atgtttttgt aataagtttg aagacgcaaa aaaataatat aataacaaaa
     1741 atcctccagc cttgtaggcc agaggataaa atacttaatt gaggaaaata tcagggaaaa
     1801 gtatttctga cttaactttc ccggctggag agtgaatcaa tgtaatcagc gtaccattgc
     1861 atcatttcac ggcggccatc gagatacagg gcatgattat aggtacccct gatggaattt
     1921 ttatccacat gagctaactg catttcaatc caggcggaat taaagccttg ttcatgcaga
//
LOCUS       DAZOZY010000350         1958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZOZY010000350 DAZOZY010000000
VERSION     DAZOZY010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.353"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..271)
                     /locus_tag="ACHURQ_004302"
     CDS             complement(<1..271)
                     /locus_tag="ACHURQ_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0825905.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
     gene            complement(315..854)
                     /locus_tag="ACHURQ_004303"
     CDS             complement(315..854)
                     /locus_tag="ACHURQ_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR0825906.1"
                     /translation="MDISLRSLARQYGYDESTVRTWVEKGMPTDTDSNARSWIVDNVL
                     KPLRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKTA
                     VRAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIGNKQEEIN
                     EDTEESTTDDTTAEDTENE"
     gene            890..1453
                     /locus_tag="ACHURQ_004304"
     CDS             890..1453
                     /locus_tag="ACHURQ_004304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825907.1"
                     /translation="MSDRNNDFSLLVKLLNGFLKKDEIKKRLYSFVKKETNDWEKWLQ
                     IEFAHYLTSKRKYELHREVTIYLDPIDYPDSKLAKVDLILREKSTPSDDYIFIEFKCT
                     KGVVPLVRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKYVTEELKGS
                     YAVFKLCECPVRYNCQCEFNEIGVVLF"
     gene            complement(1599..>1958)
                     /locus_tag="ACHURQ_004305"
     CDS             complement(1599..>1958)
                     /locus_tag="ACHURQ_004305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825908.1"
                     /translation="VLRLCRCYHAIKSFALVSLMEKLILLLLLPLTTSAAIPDFKCWD
                     IHNDSFSTDMSFYVTRYLGKMPPPYGAFHYETAPDEKFYRELVITNEAFDDGYNYGSR
                     YLNKEGTLYDDIKCKRL"
BASE COUNT          589 a          361 c          334 g          674 t
ORIGIN      
        1 tcttatcggt tactaacggc tttagtactg aactggtttc aatagctgga agaaatttac
       61 catccttgaa tttcttcact tctgattcag aactactacc aaaagcaaag ttacagggat
      121 cattagccat taggttaaat gcaattgatt gtagtactgt agttttcaaa agctgggaac
      181 aagattgaag tacaatcttt ttagtacttc gctcttgagc tatatccatt ggttcttttt
      241 ggaaactaaa cggaacccaa tcaaggccca tattcggccc gtccacaaat ttaactacac
      301 cattactaat ccactcactc gttttctgta tcttcggcgg ttgtatcgtc ggtagtactt
      361 tcttcagtat cctcgttaat ttcttcttgt ttgtttccta ttacttccat gtcatcgggt
      421 aactcaaatt ccattgagcc taattggtat aatgttttat caatatgcgt tcttaataca
      481 tcgcgtaaat cctttgcatc tgattgtgca aataattcaa gataagtttt tgatggtatt
      541 gcacgcactg ctgttttgac ctggaataga tattccgtta gtacctgttc tatatattca
      601 gtactgacca ccagcccatt cttttctgct aattctaatt ctgctaactg ccgttctgct
      661 gaaagcttct tcaaacgctc ttgttcaatt tgttcttttg tattagtatc acgcaacggc
      721 tttagtacat tatctacaat ccaacttctt gcgtttgaat cagtgtctgt cggcattccc
      781 ttttctaccc aagttcgaac tgtagattca tcgtatccgt actgtctcgc taatgacctt
      841 aatgagatat ccatatttgt tcctttttat tttatttata gggtttacaa tgtcagaccg
      901 taacaatgat ttttctctgt tagtaaaatt gctgaacgga tttttgaaga aagatgagat
      961 aaaaaaaaga ctatattctt tcgtgaaaaa agaaactaat gattgggaaa agtggctgca
     1021 aatagaattt gctcattatc taacttcaaa aaggaagtac gagctacatc gtgaagttac
     1081 gatatacctc gatccaatag attatcctga tagtaaactt gctaaagtag atttgatact
     1141 gcgtgaaaaa tccacaccct ctgatgatta tatttttatt gagtttaaat gtactaaagg
     1201 tgtagtacca ttagttaggg gcttaaatgc tgacaaaaat aaaattagtg ctataagaca
     1261 gtgtaactac aaaatgcgta attatcttgg tgtcggattt catctttttt gtgaaccaga
     1321 tgatatcgag tttatggaaa agtacgtaac tgaagaactt aaaggctctt acgccgtgtt
     1381 taaattatgc gagtgtccag taagatataa ttgccaatgt gaatttaatg aaattggtgt
     1441 ggtacttttt taaatgcggt gcgggtatga tcattttgtt cacacacaat tgattaaacc
     1501 cgctgccgaa aaccctcgat agtaccgcca gtatggaggg acccattgaa tgcaatggtt
     1561 aatatacttt gtaagctata tacaatgaat catgagtatc aaagccgctt acatttaata
     1621 tcatcgtaca aagttccttc tttgtttaga tatctcgaac cgtaattata tccatcatca
     1681 aatgcttcat tggttattac cagttccctg taaaatttct catcaggtgc tgtttcataa
     1741 tgaaatgcac cataaggcgg cggcatttta ccgagatatc ttgtaacgta gaaactcatg
     1801 tcagtactaa atgaatcgtt atgaatatcc cagcatttaa agtctggaat agctgcactt
     1861 gttgttaaag gtaataataa tagcaatatc aatttttcca taagagatac caatgcaaat
     1921 gatttaattg catgataaca cctacaaagt ctcaatac
//
LOCUS       DAZOZY010000351         1944 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZOZY010000351 DAZOZY010000000
VERSION     DAZOZY010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.354"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..726
                     /locus_tag="ACHURQ_004306"
     CDS             <1..726
                     /locus_tag="ACHURQ_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR0825909.1"
                     /translation="KHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVNVRLEN
                     KGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASTSLPKD
                     RESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPLALKWF
                     WSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTGLNGKA
                     SSAKVHFYAINDFGGAIEGNASL"
     gene            761..1944
                     /gene="htrE"
                     /locus_tag="ACHURQ_004307"
                     /pseudo
     CDS             761..1944
                     /gene="htrE"
                     /locus_tag="ACHURQ_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          552 a          421 c          430 g          541 t
ORIGIN      
        1 aaacatacaa cagctttatg cttcgtaacc tgtatggctt ttagttcatc gtctattgcg
       61 gacattgtca tttcgggtac tcgcgtaata tataaaagcg atcaaaaaag tgtcaacgta
      121 cgtctggaaa ataaagggaa taacccgttg cttgtccaga gttggttaga tactggcgat
      181 aacaacgctg agcctggcag tattacagtc ccttttactg ccacgccgcc agtatcgcgt
      241 attgatgcca aacgtgggca aacaatcaaa ttaatgtaca cagccagcac ctcactgcct
      301 aaagacagag agagcgtgtt ctggtttaac gtactggaag ttccaccaaa accagatgca
      361 gaaaaggtcg cgaatcaaag tctgctgcaa ctggcatttc gcacacgtat aaaacttttc
      421 tatcgcccgg atggattgaa gggaaatccc tctgaagccc cgttagccct gaagtggttc
      481 tggtcaggtt cagaaggtaa ggcgtcattg cgagtgacca atccaacccc ttactacgtc
      541 tcttttagca gtggtgattt agaagctagc ggtaaacgct atccgattga tgtgaaaatg
      601 attgcaccat ttagtgatga ggtcatgaaa gtcactggcc ttaatggcaa agcaagttct
      661 gcaaaagtac atttttacgc cattaatgat tttggtggcg ccattgaagg taatgccagt
      721 ctgtaatcag gcaaggatat aattccgcag gaagcataac gtgactataa aaccaactaa
      781 aaattatcat aatcatctga cccgtatcgc tacgttttgc gcgctgctgt attgcaatag
      841 tgctttctgt gctgaacttg ttgaatatga ccataccttc ctgatggggc agaatgcctc
      901 aaatattgat ctcagccggt atagtgaagg taaccctgct ataccgggta tgtacgacgt
      961 cagtgtttat gtcaacgatc aacctattat taaccaaagc attacattta ttgaaattga
     1021 aggaaaaaaa acgcacaagc ctgtatcaca cttaagaatt tattgcagtt tcatataaat
     1081 tctcccgata taaataacga aaaagccgtt ctgcttgcca gggatgaaac actcggcaat
     1141 tgccttaatt tgacggaaat tatccctcag gcttctgttc gttatgacgt taacgagcaa
     1201 cgtctggata tagacgttcc tcaggcatgg gtaatgaaaa attaccaaaa ctatgttgat
     1261 ccatcgttat gggaaaatgg cattaatgcg gccatgttgt catacaacct caacggatat
     1321 catagtgaaa ctcctggtcg aagaaatgac agcatttatg ctgcttttaa cggtgggatg
     1381 aatttaggtg catggcgact gcgtgcctca ggcaactaca actggatgac cgattctggc
     1441 agtaattatg attttaagaa tcggtatata cagcgtgata tcgcctcgct gcgttctcaa
     1501 ctcattcttg gtgagtctta tacaacgggc gaaacctttg attctgtcag tatccgcggc
     1561 attcgtttat acagcgacag ccgcatgttg cctccgacat tagcgagttt tgcgcctatc
     1621 attcatggcg ttgctaatac caacgccaaa gtcactatta cgcaaggtgg ctataagatt
     1681 tatgaaacga ctgtgccgcc aggcgctttc gtcattgatg atctgagtcc gtcaggttac
     1741 ggcagcgatc ttattgttac tgtcgaagaa tccgatggct caaagcggac attctcgcaa
     1801 cctttttcat cggtcgttca aatgttacgc cctggcgttg gacgttggga tattagcggc
     1861 ggccaggtct taaaagatga tattcaggat gagcctaatt tatttcaggc cagctactac
     1921 tacggtctga ataactatct aacg
//
LOCUS       DAZOZY010000352         1939 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZOZY010000352 DAZOZY010000000
VERSION     DAZOZY010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.355"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHURQ_004308"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHURQ_004308"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR0825910.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHURQ_004309"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHURQ_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR0825911.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT          588 a          437 c          391 g          523 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccag
//
LOCUS       DAZOZY010000353         1921 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZOZY010000353 DAZOZY010000000
VERSION     DAZOZY010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.356"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..100
                     /gene="mgtT"
                     /locus_tag="ACHURQ_004310"
     CDS             <1..100
                     /gene="mgtT"
                     /locus_tag="ACHURQ_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR0825912.1"
                     /translation="GDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHURQ_004311"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="ACHURQ_004311"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR0825913.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1753)
                     /gene="ydeI"
                     /locus_tag="ACHURQ_004312"
                     /pseudo
     CDS             complement(1360..1753)
                     /gene="ydeI"
                     /locus_tag="ACHURQ_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
BASE COUNT          555 a          449 c          374 g          543 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcca tcctgtttta
     1681 aaccgccctg gtcatctgcc aacgcataag gcatgacaag aaaacttgct aatacaatgg
     1741 actgaaattt catactaact ccttaattgc gtttggtttg acttattaag tctggttgct
     1801 atttttataa ttgccaaata agaatactgc caattgttat aaggcattta aaatcagccc
     1861 actagctgtc aaatattcag agaatttgac tcactaaagt taagaagatt gaagagtctt
     1921 a
//
LOCUS       DAZOZY010000354         1916 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZOZY010000354 DAZOZY010000000
VERSION     DAZOZY010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.357"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            190..732
                     /locus_tag="ACHURQ_004313"
     CDS             190..732
                     /locus_tag="ACHURQ_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0825914.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            812..1486
                     /locus_tag="ACHURQ_004314"
     CDS             812..1486
                     /locus_tag="ACHURQ_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR0825915.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1502..>1916
                     /locus_tag="ACHURQ_004315"
     CDS             1502..>1916
                     /locus_tag="ACHURQ_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR0825916.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          612 a          330 c          412 g          562 t
ORIGIN      
        1 attaattgat agatgcgata aatagttaat actcttaaat atttgttgta tttatattgc
       61 aatggttaat ttgtttcaaa taaataaaac aatgtgttta cattgcggta acttttttga
      121 tgtgagaaat ttgtttccaa gagatacaga ctcttaacaa ccaaaaaaaa ttttattaag
      181 gaacatatta tgaaacgttc aattattgct gccgctgtct tttcttcttt ttttatgagc
      241 gctggagtat ttgctgcaga cgttgatacc ggaacattaa ctattaaggg gaatattgca
      301 gaatctccgt gtaaattcga agcgggtggt gattcagtaa gtattaatat gccgactgta
      361 ccaaccactg tctttgaagg taaagctaaa tattctacct atgatgatgc agtcggtgta
      421 accagcagca tgttaaaaat tagctgcccg aaagaagttg ctggtgtaaa actctcgttg
      481 attaccaacg ataaaataac cggtaacgat aaggcgatag ccagtagcaa cgataccgtg
      541 ggttactatc tctatttagg tgataacagc gatgtcctgg atgtttctgc accttttaac
      601 attgagagtt ataaaacagc ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg
      661 aaactgacag ataactcagt gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg
      721 gcgcaggatt aataaataaa attgtaagtt tctccaggta ataaacatgg ccgctatccc
      781 atggcggcct tttaatttaa gaggcattaa aatgaaagga ttattatctt tactcatttt
      841 ttctatggtc cttcctgcac atgccggaat tgttatctac gggacgcgca ttatttaccc
      901 ggcagaaaat aaagaagtga tggtgcagtt gatgaaccag ggaaaccgtt cttcgctgct
      961 gcaggcgtgg attgatgatg gcgatacgtc attaccacca gaaaaaattc aggttccttt
     1021 catgttaacg ccaccagtgg caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa
     1081 aattatgccg aataaactgc ccactaataa agaaagcatt ttttatctga atgttctgga
     1141 tattccacca aatagtccag agcaagaagg taagaatgca ctgaagtttg cgatgcaaaa
     1201 cagaattaag ttgttttacc gaccagcggg tattgctccg gtaaataaag cgacatttaa
     1261 aaaattgctg gtaaatcgca gtggcaatgg tttggtgata aaaaatgact cagctaattg
     1321 ggtgacgatt tcggatgtca aagctaataa tgtcaaagtc aattatgaaa ctattatgat
     1381 tgccccctta gaaagtcaga gtgttaatgt caaaagtaat aatgcaaata actggcatct
     1441 gaccattatc gatgaccatg gcaactatat tagtgacaaa atttaattgc aggagctgcc
     1501 tatgttgaga atgaccccac ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc
     1561 ttatgcagct gaagaaacct ttgataccca ttttatgata ggtggaatga aagaccagca
     1621 ggttgcaaat attcgtcttg atgataatca acccttaccg gggcagtatg acatcgatat
     1681 ttatgtcaat aagcaatggc gcgggaaata tgagattatt gttaaagaca acccgcaaga
     1741 aacatgttta tcaagagaag ttatcaagcg gttaggcatt aatagcgata acttcgccag
     1801 cggtaagcaa tgtttaacat ttgagcaact tgttcagggt gggagctata cctgggatat
     1861 cggggttttt cgtctcgatt tcagtgtccc gcaggcctgg gtggaagaac tggaaa
//
LOCUS       DAZOZY010000355         1873 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZOZY010000355 DAZOZY010000000
VERSION     DAZOZY010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.358"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(15..59)
                     /locus_tag="ACHURQ_004316"
     CDS             complement(15..59)
                     /locus_tag="ACHURQ_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825917.1"
                     /translation="MFSKLAPSSIKAMF"
     gene            complement(295..405)
                     /locus_tag="ACHURQ_004317"
     CDS             complement(295..405)
                     /locus_tag="ACHURQ_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825918.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            complement(458..862)
                     /locus_tag="ACHURQ_004318"
     CDS             complement(458..862)
                     /locus_tag="ACHURQ_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR0825919.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(1083..1814)
                     /gene="bluR"
                     /locus_tag="ACHURQ_004319"
     CDS             complement(1083..1814)
                     /gene="bluR"
                     /locus_tag="ACHURQ_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR0825920.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          526 a          387 c          385 g          575 t
ORIGIN      
        1 tgaacatcct gtaattaaaa catagccttt atgctacttg gtgccaattt gctaaacatt
       61 atggttgcct ttttatataa cgataataat gaatataaac atgacatgag aataaggttt
      121 caatttttga gttatatagg aaaggtttaa cctgttcctg gctaaaatac atataactgg
      181 atgatgacta aaccaaaaca catgtgcgtt aagtattgaa acgggcgtat ggcacacccg
      241 ttgtttttat aaatatatta accgttataa aataacgtat agaaagtcaa gtgatcacat
      301 ttcaaatatc aatttgatag tattggcctg gtgattattt atgggtagca ataaaaggac
      361 agtatttatc atccataggg atagtctctg tacttttatt cccattatgc taatgcctta
      421 ctgaattatg aagcatttct taagcatcca actttagcta gattaatggt ttattatttt
      481 ctacatcttc aatatataaa agcgtattat caatggcgta gtaactgcgt ttgttatgat
      541 taacatcagt aacccaacgg aaaacgcccg cgcctgccag tgttgaacag tattcccgaa
      601 atgtagattt tccgtaaata tgaagcaatg cggcctcttt tattttagca gggttcttgg
      661 tcgtactaac ttttaacagg ttcctggttc ctcttaataa caaaaccgtg tcatcgtgag
      721 taataattct gatgttatcc gtagccagat aataaatgta atgtgcaata cggtgatgtt
      781 ttaattctga ataaaaccag gagaagtttt gctcttttct cacttgctca aacatctttt
      841 gaaaaacaac gacctgatcc atcaggataa taacctcttg ttagttgtga gactgcgtag
      901 tgtgcacgat cggttttacc acttcaatct ggtctgtcct ttggctgtga tatgtacaga
      961 gtgtgataga gggattacct gaattcttcc ggtgagcatt ttgcaacgga ccagctccgg
     1021 tacaaatgct gttgtgggtt cagattataa cattctgtct aaggggcggg ataaaggtga
     1081 aattaggggg catgaaagat gactttataa ccttgctcac cccaatgttg taaaagttcg
     1141 ttttgccttc tcgttggtgc catgcctgtc cagacaatca atgtttgcgt cgggaacagt
     1201 tccgggcgcg gcgattcaat gggatcagca agaacagaaa tgtgccatcc tgacaatgat
     1261 aaacgccatg cttccagcca caatcgtgct ctctcttcaa cattccacgc catcagaatg
     1321 gcttctttac cgggcttacg gcgcatttcg aaaagtgagg ctgctgcata ttcaattaat
     1381 gcgccgtcaa acatactgct cataatgcgg gaggtgttgt gatcaagcac gagacgctgg
     1441 cgaacaggaa ggtaaacatg attaatcaat tgatctactg ggtactctcg acccagtgaa
     1501 ataattctcg cgcgtagttt ggcaggatta gccatacgaa gaattgacat catctcttct
     1561 tgcaggcggt tccagtcatc ttccgtatcc tggctggtgg tttccagtaa tgctttaact
     1621 ttgcctacag ggacgccatt acttatccaa cgcttgatct cttcgatgcg ttgtatgtct
     1681 tcttcatcaa agagtcggtg tccgccttca ctgcgctgtg gttttaacaa accgtagcgg
     1741 cgttgccagg cccggagagt gacaggatta atcccgcaac gttcagcaac atcaccaatg
     1801 ctgtaataag ccacaattcc tccttgcggt cataaatctc cgccgcctgt acacgaccca
     1861 ataatacttt gta
//
LOCUS       DAZOZY010000356         1870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZOZY010000356 DAZOZY010000000
VERSION     DAZOZY010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.359"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            186..689
                     /locus_tag="ACHURQ_004320"
     CDS             186..689
                     /locus_tag="ACHURQ_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR0825921.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(768..1019)
                     /gene="yecJ"
                     /locus_tag="ACHURQ_004321"
     CDS             complement(768..1019)
                     /gene="yecJ"
                     /locus_tag="ACHURQ_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR0825922.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1134..1220)
                     /gene="azuC"
                     /locus_tag="ACHURQ_004322"
     CDS             complement(1134..1220)
                     /gene="azuC"
                     /locus_tag="ACHURQ_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR0825923.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1483..1806
                     /locus_tag="ACHURQ_004323"
     CDS             1483..1806
                     /locus_tag="ACHURQ_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR0825924.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          486 a          439 c          412 g          533 t
ORIGIN      
        1 tataaacttg atgatttaag cattttctta tacccgttca gacgttattc ttatttcaga
       61 tcatcgtcag aattgactcc acgatcacat ttcggaccgg cagaaaggaa ttattctgca
      121 aacagtaatt atggtgtttt gatttatctt gcacctctcc acttctggat ataaggatat
      181 taggtatggc aaccgctgga atgcttctca aactcaactc tcaaatgaac cgcgagtttt
      241 acgcatccaa tctctacctt cacctgagta actggtgttc tgaacagagt ctgaacggca
      301 ccgccacttt ccttcgcgcc caggcacaga gtaatgtgac ccaaatgatg cgcatgttta
      361 actttatgaa gagtgtcggc gctaccccca tcgttaaagc cattgatgtt cccggtgaaa
      421 aactgaactc tctggaagaa ctgttccaaa aaacgatgga agaatacgag caacgttcta
      481 gtacgttggc acagttagcc gatgaagcga aagaactgaa tgatgattca accgtcaatt
      541 tcctgcgcga tctggaaaaa gaacagcagc atgatggtct gttgctgcaa accattcttg
      601 atgaagtgcg cagtgcgaaa cttgcgggta tgtgccctgt gcagaccgac caacatgttc
      661 tgaatgtcgt gtcacaccag ctgcattgat catcatcggc gctaatgcat tgcgccgatg
      721 aaggttttga gaaaccgctg cctcatctgt ttgaagcagc ggttttttta atgggattca
      781 ccctgtgggg taaacttgag ttcaataagc gcgatggctt tttggattgc ccgcatggtg
      841 accgggtctg cggcggcggg atggttagta aagtcgatat tcttcagctg actggacatt
      901 ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg tttgataacc
      961 aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg ctgggacatc
     1021 gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc ttcagcgata
     1081 gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg
     1141 aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa catacttttc
     1201 aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt gttattgtct
     1261 ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac aaatcacata
     1321 aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat ttctgacacc
     1381 tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc gcagcgctgt
     1441 aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact tctgattctg
     1501 actctttcat taataacgct ggcggggtgt acggttactc gccaagccca tgtgagcgaa
     1561 gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt gcagcatgcc
     1621 cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca ggaaggctat
     1681 acccacgcgg tcccctttgg tcagccagta ggcaactgta gcctttttgc gggttcctta
     1741 tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt ccctgtcttt
     1801 ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc ggtaatgcgt
     1861 tttattccca
//
LOCUS       DAZOZY010000357         1727 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZOZY010000357 DAZOZY010000000
VERSION     DAZOZY010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.360"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..355)
                     /locus_tag="ACHURQ_004324"
     CDS             complement(<1..355)
                     /locus_tag="ACHURQ_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR0825925.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(355..849)
                     /locus_tag="ACHURQ_004325"
     CDS             complement(355..849)
                     /locus_tag="ACHURQ_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR0825926.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1207..1383
                     /locus_tag="ACHURQ_004326"
     CDS             1207..1383
                     /locus_tag="ACHURQ_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825927.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1525..1677)
                     /locus_tag="ACHURQ_004327"
     CDS             complement(1525..1677)
                     /locus_tag="ACHURQ_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825928.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1665..>1727)
                     /locus_tag="ACHURQ_004328"
     CDS             complement(1665..>1727)
                     /locus_tag="ACHURQ_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR0825929.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          475 c          399 g          412 t
ORIGIN      
        1 tggcgagtcg ttttttggag tgttcgcggg ctttttcctc cgtcatctga ataatcagca
       61 tatcagcagg atcgcaaatc acgttgtaaa tcacccagcc gtcaatcagg ccgatagtct
      121 tgccggttcg tgccgggcca acaaatatca ctgcgtcgta ttcacgcgag gccaggcagt
      181 tcatcggctc aataacatac ggtgccacca gcggatccca cgggactgag ttccctgccc
      241 ccatgggcac ccgcatatac tgagcaacgg catcagcaac ccgcattcgt ctcggtgcgc
      301 gaaggatata acctgaatcg gttcgtgctg cctttgcggt ttcctgattc agcattactc
      361 ctcctgctgt aattcctcct catcatccgc acctgcttca gtcacccgca gggctatctg
      421 atcgcgcaga tcatcaataa tggactgaac acggctcaca gcggcaggct gcagaccgca
      481 gtcacgttca agaatatccg gtaatgtctc cagcacctgc acgaccgctt ttgcccagat
      541 ggcaaactcc cgtctgacat cactggccgg aatgagttgt gccgtttcct gttcgaactt
      601 aagacgctca cgttcagact gataccaggc tttgcgctca tgcgcgtcca tttcgccttc
      661 tgcaaccggc ggtggtaatg ccagaaatgc cgacacaata tcaaccaccc gataaagctt
      721 gaggttgctt tcatgccccc ctgcaacggg tagattttgc agccttgccg cagcagtctg
      781 gcgatgtaca cctgacagtg ccgccagttg actgatattc agcgtcagat tttttaactc
      841 tcgatccata cccgctccag aatgttttaa acatgcatct tgcgaacaac tttaggcaaa
      901 cggtgttagt gatgaacaaa aaacaatcaa aatcgacacc ataaaaataa aaccactgta
      961 atatcaatcc attacagtag tggtgatgac gaatgaaatt tcaaaaacta gcctttttcc
     1021 gcgccgctcc cgccccgtgg caggccaccc caccgggagg acccgtcagc ctgacagcca
     1081 tgacgaacgt ctgatacagc gccctgcatg aatgacatcg ggataatcca gaaaggaata
     1141 gcatcgtacc cacaagaatc agtgtaagtg tcctgtttct tccacccccg cacaggactg
     1201 gcgagcatga gggacaaacc cgcgaaccat aaacgcggta aaaacccggt gtgcatcgtt
     1261 tttgattatt cccgcacact cgcgcagaag gagttccccg tcgggctacg gtcatggtta
     1321 atacgggaat acggcgacga tacagcgcat gatgtgtcag gcttgaatac ctttatccgt
     1381 taaaagggat atcagttaag ttatcccgtg tagggtataa gccattatca aagccactct
     1441 gtaggaagtg gcttttgtaa tggcaataaa aagccccgcg aatgcgaggc taaatcctgg
     1501 tatttgtaat gactggctct tatctcaacg cagcccctta ccgcgcgcaa aatgctcaat
     1561 atcaagcatc agcaatgaga tgtttaatct ggattcactc cagaagtgag caccaccctg
     1621 tctacagagc cagatgtgaa ggatgatgag taaaattatc gctatcatcg aaggcattgc
     1681 gtcctgatgt attcctgaag cgttctcagt gctgtttggt cgcggat
//
LOCUS       DAZOZY010000358         1726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZOZY010000358 DAZOZY010000000
VERSION     DAZOZY010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.361"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..233
                     /locus_tag="ACHURQ_004329"
                     /pseudo
     CDS             1..233
                     /locus_tag="ACHURQ_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            458..949
                     /locus_tag="ACHURQ_004330"
     CDS             458..949
                     /locus_tag="ACHURQ_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0825930.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            1284..>1726
                     /gene="ygeH"
                     /locus_tag="ACHURQ_004331"
     CDS             1284..>1726
                     /gene="ygeH"
                     /locus_tag="ACHURQ_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFR0825931.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISN"
BASE COUNT          658 a          268 c          268 g          532 t
ORIGIN      
        1 acagatgatt taatgagtat ggctgtaaat gaactcataa aactgaccaa aatagagaat
       61 aatgtagacc tgaataaatt cactactata tgcacagacg ttctatcccc cctcgtcacc
      121 agacataata aagaaaaaaa acaaacgaca ttctaccctt ctcaaaaatc ccctttttaa
      181 ttttcattga aaaaatggca attgatacta tgaatccagg tcatacacat taaggcattt
      241 atgtaatgtc gtaattaaga taactaataa ggtgaatatt agtaaaacaa ctaatgatgt
      301 tattagtttg tagtgaacag tacttttacc aataatgaaa aatataccat aagcaatgca
      361 agcttaaaac taataattta aaaaagataa atgtaaaaat attaatgagt caatcaataa
      421 taatttagca tcacgagaca catcacagag gaatattatg agcacagaaa caattgaaat
      481 attcaataat agtgatgaat gggcaaatca actaaaacac gcattatcga aaggagaaaa
      541 tctggcatta ctacatggtt taactcctga tatccttgat agaatatatg catatgcatt
      601 cgactaccat gaaaagggta atataacgga cgcagaaatt tattataaat ttttgtgcat
      661 ttatgcgttc gaaaatcatg agtatctaaa agattttgca tcagtatgtc agccaaaaaa
      721 gaaatatcaa caagcatatg acctttacaa actaagttac aattactccc cgtatgatga
      781 ctattcagtt atttatcgta tgggtcaatg tcagattggg gctaaaaata tcgataacgc
      841 aatgcaatgt ttctatcaca ttattaacaa ttgtgaggat gatagtgtta agagtaaagc
      901 gcaggcatat attgaactct taaacgataa ttcagaagat aatggctaag cattacaatc
      961 atttggttaa atttgctggt tatagtgcag ttaactacta taaccagcac ctcttatgtt
     1021 ttcttaaatc agcatcttaa aacagttaag agcatactat aaatcattat ttttcaatgt
     1081 gttagagaac aatctacttt ataaaaattg aataaggcta tttcagatta catcactgaa
     1141 ttcctaagat aaattagtga acacaaccat agatatgtcg aatgtatttt cctgtagcat
     1201 taatgacaca taaatatgaa tagccataat ttctattgct taagcaggat gcaagaaacc
     1261 aattttttca tagaggttaa ctaatggact tagaaaataa attctcatat cattttcttg
     1321 agggattaac gctcacggaa gatggaattc ttactcaagg aaatgagcaa gtttatattc
     1381 cacagaaaga gttaggtgta ttaatagtat tacttgaatc cgctggtcat gttgtactga
     1441 aagatatgat catcgaatca gtatggaaaa atattattgt tagtgacgag tccctgacaa
     1501 gatgtatcta ttctttgcgc tgcatttttg aaaaaattgg ctatgatcgt tgcatagaaa
     1561 caatctaccg gaaaggttat cgtttcagcg ggcaggtttt caaaactaaa attaatgaag
     1621 ataatacttc agactattcc atagctatat tccctttcac tacttcattg aatacactgg
     1681 atccattaat acttaatcag gaattagtgc aaatcatttc aaataa
//
LOCUS       DAZOZY010000359         1712 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZOZY010000359 DAZOZY010000000
VERSION     DAZOZY010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.362"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(108..551)
                     /locus_tag="ACHURQ_004332"
     CDS             complement(108..551)
                     /locus_tag="ACHURQ_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR0825932.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="ACHURQ_004333"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="ACHURQ_004333"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR0825933.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          413 a          482 c          386 g          431 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc ga
//
LOCUS       DAZOZY010000360         1674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZOZY010000360 DAZOZY010000000
VERSION     DAZOZY010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.363"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>1674
                     /locus_tag="ACHURQ_004334"
     CDS             <1..>1674
                     /locus_tag="ACHURQ_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFR0825934.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLR"
BASE COUNT          582 a          327 c          312 g          453 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agag
//
LOCUS       DAZOZY010000361         1629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZOZY010000361 DAZOZY010000000
VERSION     DAZOZY010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.364"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHURQ_004335"
     CDS             <1..93
                     /locus_tag="ACHURQ_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0825935.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            104..292
                     /gene="dicB"
                     /locus_tag="ACHURQ_004336"
     CDS             104..292
                     /gene="dicB"
                     /locus_tag="ACHURQ_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR0825936.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            289..480
                     /locus_tag="ACHURQ_004337"
     CDS             289..480
                     /locus_tag="ACHURQ_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR0825937.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            574..>1629
                     /locus_tag="ACHURQ_004338"
     CDS             574..>1629
                     /locus_tag="ACHURQ_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR0825938.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          361 c          391 g          379 t
ORIGIN      
        1 cggttttatc tggaagaaca gggatggact cttgttaacc ggggcgcaat taaaaatatg
       61 actgtagagc aattactcaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
      121 aaacgttaat acgactgaag gttgttttga aattggtgtc actatcagta acacggtatt
      181 tactgaagat gccattaaca agagaaaaca cgaacgggag tcattaaata aaatatgcat
      241 tgtttcaatg ctggctcgtt tgcgtctgat gcaaaaagga tgctggcgat gaatacagca
      301 tttgcactcg ttctgaccgt tattcttaat acgggcgaac cagctgaact ggttaccgga
      361 gtatacaact ccatgaaaga atgcgtggct gctgcagctg aacagaaaat tcccggtaac
      421 tgttatccgg ttgataaaat cattcaccag gataataaag aagtcccggc aggtctttaa
      481 agcagccacg taataaatat ccggtttcat ttttatatgc cagcaatggc agggatttgt
      541 tcacccttaa atctgtaatg aggttaaaac aaaatgagta aagtctttat ttgcgccgcc
      601 attccggacg aaaaggcaac aaaagaagaa ggcgctaaac ctgtagccac tgccattgaa
      661 gccggcgacg aacgccgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg
      721 gctgctcagg actgcgctta taaatttatt gtctgcgagg ataaacctgg tataccccgc
      781 cctgccctcg attcctggga tgctgaatat atgcaggaaa accgctggga tgaggagtct
      841 gcttcctttg tcccggttga gactgaatcc gaaccgatga acgtcacttt tgacaagctg
      901 gcccctgaag tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt
      961 gatatggtga ttagcgcgca ggaactgttg caggaagaca tggcaacatt cgacggacat
     1021 atcgttgaag cgttgatgaa aatgccagaa gttaacgcca tgtgtccgga gattaaactg
     1081 catgccatcg ggtggattaa gcataaatgt aagcctggtg caaaatggcc agaaattcag
     1141 gcagagatgc gcatctggaa aaaatgtcgc gaaggtgaac gcaaggaaac cggaaaatac
     1201 acgtctgttg ttgatctcgc ccgtgccaga gccaatcaac agcacactga aaattcaaca
     1261 ggaaaaatca gcccggccat tgctgccatt catcgcgaat acaagcagac atggaaaaca
     1321 ctggatgacg aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac
     1381 ggcagcatcc atcgctgggc aaaaaatgaa gttatcgaca acgaccgcga agactggaag
     1441 cgtatctcgg catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt
     1501 tttggccttg tccgtgaacg tccgatcgac attcacaaag accctgtggc actgaacaaa
     1561 tacattactg aatacctgac tacaaagggc gtgtttgaag atgaaggaac aaatcagagc
     1621 gcaactgat
//
LOCUS       DAZOZY010000362         1625 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZOZY010000362 DAZOZY010000000
VERSION     DAZOZY010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.365"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..1436
                     /locus_tag="ACHURQ_004339"
                     /pseudo
     CDS             1..1436
                     /locus_tag="ACHURQ_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
BASE COUNT          460 a          419 c          394 g          352 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agcgaactga ggtgtactgg caatagcgga
     1561 cactaccatt tgttcttttt ttaagcagcc atctgatgat atttttccct gaaggctgcc
     1621 gggga
//
LOCUS       DAZOZY010000363         1598 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZOZY010000363 DAZOZY010000000
VERSION     DAZOZY010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.366"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            329..961
                     /locus_tag="ACHURQ_004340"
     CDS             329..961
                     /locus_tag="ACHURQ_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0825939.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            1295..>1598
                     /locus_tag="ACHURQ_004341"
     CDS             1295..>1598
                     /locus_tag="ACHURQ_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR0825940.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT          539 a          285 c          287 g          487 t
ORIGIN      
        1 agagatctga acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat
       61 cttctggtca ttcaaaaaca atttgcgata tagatattct cataccatga gatagttatc
      121 tggaccacgt tcgaccatgc aagattaaat cagtgaaaca taatcatatt taataacgag
      181 aaatgcattt ttaaatgtct taatcagaat tatagcaaat acagattaat cgcaattttt
      241 cccagcaaaa ttacaaaatc aagatgtatt gacatcatca atcaccaatg aaattatgca
      301 attatatacg gatagggagg ttcttaacat ggggctttgt agtcgttata aaagtcttac
      361 atgcaatagt tgctcaatgc attgccaaat aatgccagaa gagtcaccgc gtttgcagta
      421 ttgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt tcatatttta atcgtggtgt
      481 ggtggaaggt atcttaacaa aaaaccacaa tgcgagatta agcggatata tttttgtgga
      541 tttttcagta agttttttac gtctattcct tgaaaaagac tggattgact atcttgcaag
      601 tactgacatg gggatagttt tagtcagcga ccgcaatatg caatcgttag ctaactactg
      661 gcgaaaacac aacaccgcaa tatctgctgt catatataat gatgatggtc ttgatgttgc
      721 caatgaaaag atcagacaac tatttattgg tcgttattta tcatttacca gaggtaacac
      781 attaactcag atggaattca ccataatggg gtatatggtt tctggttata atccatatca
      841 gattgctgaa gtgctggata tggatatccg tagcatctac gcgtacaagc aacgaatcga
      901 aaagagaatg ggtggtaaaa taaacgaatt atttattcgt tcacattcgg tccaacattg
      961 atactcaacg accagccaga atcatactct gttcatacgg gcatctattg aaatgcccgt
     1021 attttataaa taagcagtaa ccccaagagt aaacaacata gtatttaaaa ccatcacacg
     1081 aaaaattcag aagcattacg aaatatgaat ttacaaacaa tagtggcata aatgttaacc
     1141 atgttaattt acgtaaagtt ttacgttgca acattaaagc ctcatttcaa tcatcatgat
     1201 aaatataaaa ttaatatata tttatgccgt aaataccaat atacttagca aactatgtga
     1261 tctccatttc gattgattta gtgtttattg acgtatgtac tggattatta acgataatat
     1321 cgagttctgg cctgagcacc gaaaattaat atcggtacat aacgccgatc ttaacgtcgt
     1381 tctgacaacg ccagccagtc gatgtttatc acttctactt gaagcttttc ctgatgtggt
     1441 tacacaacaa gattttttca ccagagtctg ggaagaagaa ggtatgcgtg tgcctactaa
     1501 cacgttatat cagaacatat ccattatcag acgcggattt cgcgctgttg gtgatactac
     1561 ccactcgcta attgcaaccg tgccgagaga ggattcaa
//
LOCUS       DAZOZY010000364         1590 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZOZY010000364 DAZOZY010000000
VERSION     DAZOZY010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.367"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1383
                     /locus_tag="ACHURQ_004342"
     CDS             <1..1383
                     /locus_tag="ACHURQ_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFR0825941.1"
                     /translation="PSIKSSTHIALLFMVSMGTQKLKAQSFFIFSLLLTLILFCITTL
                     YNENTNVKLIPQMNYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLI
                     YLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNK
                     KKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLW
                     AFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKL
                     FVVSFLIYNIFQKLQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDI
                     NQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKI
                     IAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSE
                     GGNKVIIHHI"
BASE COUNT          438 a          333 c          297 g          522 t
ORIGIN      
        1 ccatcaataa aatccagcac tcacattgct ctccttttta tggtttctat gggtacacaa
       61 aaattaaaag ctcaaagctt ttttattttc agtttattgc tgacgttaat tttattttgc
      121 attactacct tatataacga aaacacaaat gtaaaactca tcccacagat gaattacctg
      181 atggttgttg tggctttgtt tttccttaac gccgtcattt ttcttttcat gttaatgaaa
      241 tatttcacta acaaacaaat tttaccaaca ctcattttaa gccttgcatt tttaagtggc
      301 cttatctatt tagttgaaac cattgtaatt atccataaac caattaacgg cagtacactg
      361 atccagacaa agtcgaatga tgtttctatt ttctatattt tccgccaact cagttttatt
      421 tgtttaacct cgctggcact cttttgttat ggaaaagaca acatccttga caacaataag
      481 aaaaaaacgg gaatcctgtt gctggcgctg attccttttt tagtttttcc ccttctggca
      541 cacaatctga gcagttataa cgctgactat tctttgtatg tcgtcgacta ctgtccggac
      601 aaccatactg cgacctgggg aatcaactat acaaaaatat tggtttgtct gtgggcattt
      661 ttactgttct ttattatcat gcgtacacga ttagccagcg aactatggcc gttaatagca
      721 ttattatgtc tggcatcgct atgctgcaac ttacttctac tgactctgga tgagtataat
      781 tacaccatct ggtatatcag tcgcgggatt gaagtttcca gtaaactgtt tgttgtgtct
      841 tttctgattt ataacatttt tcagaaactg caactctcca gcaaactggc agttcatgat
      901 gtgctgacca atatttataa tcggcgctac tttttcaaca gcgtagagtc attattgtcg
      961 agacctgttg ttaaggactt ctgtgtcatg ctggttgata ttaatcagtt caaacgcatc
     1021 aatgcccaat ggggacatcg tgtgggtgat aaagtgctgg tttcaattgt cgatattatc
     1081 cagcaaagca tccgccccga tgatatttta gcgcgactgg agggtgaggt gtttggcttg
     1141 ctatttaccg aactcaatag tgcccaggca aaaatcattg cggaacgtat gcgtaaaaat
     1201 gtcgaactcc tgaccggctt tagtaacaga tatgatgttc ctgaacaaat gaccatcagt
     1261 attggcacgg ttttttcaac gggtgacacg cgtaatatct cgcttgtcat gacggaagca
     1321 gataaagcct tacgcgaagc gaaaagcgag gggggcaaca aagtgattat tcatcatatt
     1381 taagtgcaaa aatattcaga gccatgcttt ttgcgtggct tttgcataca atttattact
     1441 attaccccta aatttcccct ccccagcggc gcagagatga gtataattag cgcccctgtg
     1501 ccaggccgca atcgaacttt atctggtttt ctcgtttcac taaccgaagg agtgccattt
     1561 atcatgaaat tgcaccatag aatgctccgg
//
LOCUS       DAZOZY010000365         1575 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZOZY010000365 DAZOZY010000000
VERSION     DAZOZY010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.368"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            289..1404
                     /locus_tag="ACHURQ_004343"
                     /pseudo
     CDS             289..1404
                     /locus_tag="ACHURQ_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          623 a          286 c          221 g          445 t
ORIGIN      
        1 cacatctaaa acatcacaaa aaaaatcact tcacacacta tcagagaaca taataacttt
       61 ttaaaatgag cgcattttga tagttgttaa cgatttcctt tacgatacta tcactaccct
      121 ttttttatac acaaatatta ccgggtggag ataaaaggga aatcaaaggt aattatatca
      181 ggaagatata acgcattaca tttattgtgt gtagaagaaa atccattcct gttctaatta
      241 tccaatttaa acatcttagc ataaaacaaa tgatgaataa ggaattttat gtcaattgac
      301 tttaccccag gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc
      361 gataatgcta aaacgccaga cacaccgaag tggccttgcg ataattggga agaacaacaa
      421 cccattaatt ccacattctc tggagaagga tatatctctg atcaatatga tttagtgcaa
      481 caccaacttc aacaaattaa tgcgtgccac acgaacacta catatacaaa tgcagactac
      541 tcaaaagttg tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca
      601 caacttacgc aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa
      661 aacaaaagtg ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc
      721 ataactaaaa ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt
      781 tcagatacta attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt
      841 caaagcctgt atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg
      901 atgaaagtct ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc
      961 gggttcgttc taaccttgtc aaggttttat ttcgaagatc tacttaatat aaatttcact
     1021 gacgcaaatt taggtgacac tattttttac ataaagaaca ccctaccccc aaattatata
     1081 aagatggaca atatcttgac aaacaaatcg aaggtttatt ttcaacatta ttaaccatta
     1141 atgacaactt tttacgagca aaagcggaaa ttgcttcgac aataattaaa tttttagagg
     1201 ccaggataac caatctttca tacaatgaca tattaaaata tcagcaagaa ttccaaaaac
     1261 aatgttataa gcaagttaaa gcctttacga cactctctcg atacaataaa attcaaacat
     1321 gggcagaaat gtctgaatat cagttcgaag tatttcaata cgagacgctt aaccccaaaa
     1381 aaatgtcaca cacgccttat ttaaagaggc cacttcctaa tgaaaaagat ataaattatg
     1441 gggtggaaat agaaatacca tctggcaaaa gaattcgctt atcaaatcat tatcagaata
     1501 taataccata atattgatta ttagcacttt ataattattg aatgaaaata gattttatgt
     1561 aattttaaaa caaag
//
LOCUS       DAZOZY010000366         1570 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZOZY010000366 DAZOZY010000000
VERSION     DAZOZY010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.369"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            211..>1570
                     /gene="istA"
                     /locus_tag="ACHURQ_004344"
     CDS             211..>1570
                     /gene="istA"
                     /locus_tag="ACHURQ_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR0825942.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PER"
BASE COUNT          398 a          438 c          393 g          341 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgcctga ccttcctggg cggcgtaccg aagctgctca tccccgataa cctcagaagc
      841 gcagtgaaaa aagccgatcg ctatgagcct gtcatcaacg acagttatca ggcgctggct
      901 gagcactatg gcacggttat tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa
      961 gcagaaaacg gcgtcctcat cgtcgagcga tggcttcttg cccgcatccg taatgaaacc
     1021 ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga catgaataac
     1081 cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat gctggatgca
     1141 ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa agccgtgaag
     1201 gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc acacgaactg
     1261 gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg gcacaagggg
     1321 cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac caatccgctt
     1381 cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct tatcgagcag
     1441 ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa agccaaaccc
     1501 catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa aaaatacggc
     1561 ccggaacggc
//
LOCUS       DAZOZY010000367         1566 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZOZY010000367 DAZOZY010000000
VERSION     DAZOZY010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.370"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..192
                     /locus_tag="ACHURQ_004345"
     CDS             <1..192
                     /locus_tag="ACHURQ_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR0825943.1"
                     /translation="GIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNEC
                     FNNRCTLQICPYGLKQQSP"
     gene            complement(473..604)
                     /locus_tag="ACHURQ_004346"
     CDS             complement(473..604)
                     /locus_tag="ACHURQ_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR0825944.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1104..1180)
                     /locus_tag="ACHURQ_004347"
     tRNA            complement(1104..1180)
                     /locus_tag="ACHURQ_004347"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1144..1146),aa:Arg,seq:tct)
     gene            complement(1281..1356)
                     /locus_tag="ACHURQ_004348"
     tRNA            complement(1281..1356)
                     /locus_tag="ACHURQ_004348"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1321..1323),aa:Ile,seq:cat)
     gene            complement(1459..>1566)
                     /locus_tag="ACHURQ_004349"
     CDS             complement(1459..>1566)
                     /locus_tag="ACHURQ_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0825945.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          484 a          411 c          291 g          380 t
ORIGIN      
        1 ggcatcatgc tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat
       61 aaaacattct ggaattcaat caaaaatagc caggaatgcc ctacaaatac agataacgta
      121 ttgaatgaat gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa
      181 caaagtccat aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag
      241 aggggctgga gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc
      301 agtaccagca cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca
      361 cagggggatt caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca
      421 accgcctgaa ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt
      481 ctggcgtttt ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac
      541 cgtcagccag cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa
      601 tcacttttta ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg
      661 aagttcatcc ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa
      721 ttctgaatta gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat
      781 aataacgtct cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa
      841 ggcctttgct cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga
      901 cgcctcatca atattcactc tacccacacc atatgaatga taaatattta atgctgaaaa
      961 aaccattaga ccgtataaca aacactcaat caatacttaa cagaactttt atttttgaca
     1021 aacataaaat attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg
     1081 gaaggaatct ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg
     1141 cttagaaggc aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg
     1201 ccatcgggga cccgcccccg caccaacaac cctgttatca tgtcgtctgc tcttcctgat
     1261 aagctaatgg cggtttgtga tggtggccct tgctggattt gaaccagcga cctggcgatt
     1321 atgagtcgct cgctctcacc actgagctaa agggccggga gcagaataat aacggtccgt
     1381 aattaattcc gcaataaaaa acccgctcgg cggcgggttg tagaaactct tctaacgtca
     1441 ggcataaaac gcccatagtt atgacgaatt taccacagat tccggaaaaa tcaacctagt
     1501 tacctttttt aactgccgct cagcccatgc ttcttcaata tcaaaccggg tcaccagcgc
     1561 atcata
//
LOCUS       DAZOZY010000368         1551 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZOZY010000368 DAZOZY010000000
VERSION     DAZOZY010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.371"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            119..1551
                     /gene="rhsC"
                     /locus_tag="ACHURQ_004350"
                     /pseudo
     CDS             119..1551
                     /gene="rhsC"
                     /locus_tag="ACHURQ_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          385 a          445 c          472 g          249 t
ORIGIN      
        1 acagtggaaa gcagcgaaaa aacgagttaa aaagcggtag aaaaagttaa gttcccggtt
       61 gatcgaatgg atgcgaatcg ctgatcgaac ttcgatgcca atccgtgatc gaatagagcg
      121 gaatacgcag ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc
      181 catttccggc gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata
      241 tggccgggac aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc
      301 ggagaattta cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc
      361 ggcagtgtat gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg
      421 gggccggatg gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag
      481 cgacgggcgg gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga
      541 gaaggaccgc atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg
      601 tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag
      661 tcagtttgat gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga
      721 gtacagcccg gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc
      781 atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct
      841 ggaaatgcgc cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg
      901 cgatatcatc cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga
      961 tgccaccggc agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac
     1021 cgactgttcc ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt
     1081 gcaccgcgag gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc
     1141 cgtgaaagac acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac
     1201 caccgtcatt gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc
     1261 tatctgtacc acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt
     1321 catccgcctg accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct
     1381 gctgatacag gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg
     1441 caaacttatc cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga
     1501 ccgcctcacg caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAZOZY010000369         1525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZOZY010000369 DAZOZY010000000
VERSION     DAZOZY010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.372"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            97..360
                     /locus_tag="ACHURQ_004351"
                     /pseudo
     CDS             97..360
                     /locus_tag="ACHURQ_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(397..954)
                     /locus_tag="ACHURQ_004352"
     CDS             complement(397..954)
                     /locus_tag="ACHURQ_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR0825946.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="ACHURQ_004353"
     CDS             complement(951..1226)
                     /locus_tag="ACHURQ_004353"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR0825947.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="ACHURQ_004354"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="ACHURQ_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          325 c          367 g          409 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAZOZY010000370         1506 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZOZY010000370 DAZOZY010000000
VERSION     DAZOZY010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.373"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..940)
                     /locus_tag="ACHURQ_004355"
     CDS             complement(<1..940)
                     /locus_tag="ACHURQ_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0825948.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLHLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQH"
     gene            complement(966..>1506)
                     /gene="rfaY"
                     /locus_tag="ACHURQ_004356"
     CDS             complement(966..>1506)
                     /gene="rfaY"
                     /locus_tag="ACHURQ_004356"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR0825949.1"
                     /translation="TKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSA
                     GLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHA
                     LNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYY
                     SVIYRTKLRKFIKKLKGKA"
BASE COUNT          467 a          319 c          262 g          458 t
ORIGIN      
        1 catgttgata tttcacttga tactgctttt cattcgtggc tggaataaac gccacatcat
       61 tccagcaaga agcctgataa gcctcattaa aatacttctg gcagggataa ttcgtccagt
      121 cgttccaggg cttcgttacg ccaacataat gaatcagctt agtatcatcg gtaatggtct
      181 ttttataatc ttcatcgctt ttatttttta actcataatc gatgccataa aagcaatcat
      241 aatcgccgct aataaaaata caatgaccaa agaacagaat atttaaaata tcctgatcaa
      301 ggcatttata cttcgtgtgc tgcgggtcac ttgccagcat agcaatggct ttattcataa
      361 aatcattttt cgcccatgct tcaagatgca gatatactac accggcgttg aaataattcc
      421 ctggaagtcc ctctatagcc agacgcttcg ctggtttttc ctgcataaaa ggcacatctt
      481 taataacagc tgcaaactta tccccatcaa aattaatatc aagcaatcca gctaatgagc
      541 ctttacagat gacatcagca tcgatatata acagattttc aatagtttca ctcagatact
      601 ggaacgcaac taaacggaag aatgttgcat aagaccactg tttcaggctg gggaagatat
      661 caataaaggc actggagact ttataaattc ttacttcaac gttagcattt ttgctggtaa
      721 aagcattaac acgttgtaga taatcttcat caatgtagtc ggtaaagaga tgaaacttaa
      781 ccgcaaggtc cggattatgc ataacaactg attgcataga tactgccgca ccaaataaaa
      841 agttacggtc aataccgtaa gcgatattta acacacgatc ggtgttagca atttccccag
      901 agacagcgac ttcagtaata ctctcagcta ataaatccat gttggttccg ttttgactgt
      961 gtggtttacg ctttgccttt taatttttta ataaatttgc gtagtttggt tctgtagatt
     1021 actgaatagt agccataatc ttttatttcg ttggcaatac ccaggtgacg ttccatggcc
     1081 agacgatcac gagctttacg ctctgccgta cagcttttac cggataggtc aattatcctg
     1141 acaccatctt tactaacgat aaagttaccc cggtgtgggt cgcccgatag catatttaag
     1201 gcatgcaatt tttccattga agccttaatt tctgctttaa catcttctgg aatgatcggc
     1261 atatcattga gctctacgcc ctcgacatac tcgataagca tgataaaaac actggcatag
     1321 ttgaaaattt tacgctcagc cagaaaataa aaatcattag gaaaagtaag acctgcactt
     1381 ctgacacgat cggtttcaac gatcaaattc tgatagtagt caccttttac aaaagatttt
     1441 aaaaaacgtt cattcctttt cgttttcggg gcaaagactt taaaaacata gcgaccatat
     1501 ttcgta
//
LOCUS       DAZOZY010000371         1482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZOZY010000371 DAZOZY010000000
VERSION     DAZOZY010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.374"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHURQ_004357"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHURQ_004357"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0825950.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHURQ_004358"
                     /pseudo
     CDS             complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHURQ_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          414 a          324 c          370 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcgaatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tc
//
LOCUS       DAZOZY010000372         1472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZOZY010000372 DAZOZY010000000
VERSION     DAZOZY010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.375"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1104
                     /locus_tag="ACHURQ_004359"
     CDS             <1..1104
                     /locus_tag="ACHURQ_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR0825951.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          359 a          332 c          390 g          391 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ctattttctt ct
//
LOCUS       DAZOZY010000373         1471 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZOZY010000373 DAZOZY010000000
VERSION     DAZOZY010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.376"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHURQ_004360"
     CDS             <1..122
                     /locus_tag="ACHURQ_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825952.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHURQ_004361"
     CDS             119..376
                     /locus_tag="ACHURQ_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825953.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHURQ_004362"
     CDS             376..750
                     /locus_tag="ACHURQ_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR0825954.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1471
                     /locus_tag="ACHURQ_004363"
     CDS             747..>1471
                     /locus_tag="ACHURQ_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR0825955.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRAS"
BASE COUNT          406 a          312 c          424 g          329 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaaggt ccagtatccg gatggtaagc cgccggtatg ggcaaagtgt acgaaaggtg
     1201 tgtatccgtc ttactgggaa gaatggaaaa aagtcaggga ggtggtaaaa gttgcctgtc
     1261 cggagtgtgg cggaaagggt gaggtttcca ccgcctgtaa ggattgccgt gggcgtggtg
     1321 tcgccattca tcgtgaagag tcggtaaaac gtggtatgcc tgttatcaga gactgccagc
     1381 gttgtggtgg tcgtggctat gaaagactac catcaacgga ggcatttaat gctatatgcg
     1441 aggtgacaaa ccagataaca cgcgcgtcat g
//
LOCUS       DAZOZY010000374         1428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZOZY010000374 DAZOZY010000000
VERSION     DAZOZY010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.377"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHURQ_004364"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHURQ_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR0825956.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHURQ_004365"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHURQ_004365"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR0825957.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1428)
                     /locus_tag="ACHURQ_004366"
     CDS             complement(822..>1428)
                     /locus_tag="ACHURQ_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR0825958.1"
                     /translation="KGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFC
                     FGKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGAD
                     EEALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVP
                     GAEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          324 a          397 c          396 g          311 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgccttta
//
LOCUS       DAZOZY010000375         1402 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZOZY010000375 DAZOZY010000000
VERSION     DAZOZY010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.378"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..107)
                     /locus_tag="ACHURQ_004367"
     CDS             complement(<1..107)
                     /locus_tag="ACHURQ_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825959.1"
                     /translation="MTSREARLGMFLASLLRRIAFSYYDYKAYNFNIEKT"
     gene            395..>1402
                     /gene="shiA"
                     /locus_tag="ACHURQ_004368"
     CDS             395..>1402
                     /gene="shiA"
                     /locus_tag="ACHURQ_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR0825960.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT          309 a          331 c          338 g          424 t
ORIGIN      
        1 gttttttcaa tattgaaatt ataagcttta taatcgtagt aactaaatgc aatacgtctc
       61 aacaatgatg ctaaaaacat acctaacctc gcctccctac tggttataat gcaatgcagt
      121 ctatcagact catcagggtg ccattttgtg catatgcgga cttttatgtt tcatatctct
      181 aacctgtggg tcctctgctt aatccttaaa caacaccagc aactcctgcg ctttcatctt
      241 ccatcgaatt tttcatgttg ccgctaatca gccataaaat catttgcaga tgcgctctgt
      301 cgaggtagtc tcataaggtt cgtttataga tcgacggcaa tgtgagttac cttttccata
      361 ctaattataa aaagacagta caaacaggat cattatggac tccacgctca tctccactcg
      421 tcccgatgaa gggacgcttt cgttaagtcg cgcccgacga gctgcgttag gcagcttcgc
      481 tggtgccgtc gtcgactggt atgatttttt actctatggc atcaccgccg cactggtgtt
      541 taatcgcgag tttttcccgc aagtaagccc ggcgatggga acgctcgccg catttgctac
      601 ctttggcgtc ggattccttt tccgtccgct cggcggtgtc attttcggtc actttggcga
      661 ccgactggga cgtaagcgca tgttaatgct gaccgtctgg atgatgggca tcgcgacagc
      721 cttgattggt attcttcctt cattctcgac cattgggtgg tgggcaccta ttttgctggt
      781 gacactgcgt gtcattcagg gatttgccgt cggcggcgaa tggggaggcg cggcgttgct
      841 ttccgttgaa agtgcaccga aaaataaaaa agccttttac agtagcggtg tacaagttgg
      901 ctacggtgta ggtttactgc tttcaaccgg actggtttca ttgatcagta tgatgacgac
      961 tgacgaacag tttttaagct ggggctggcg cattcctttc ctgtttagca tcgtactggt
     1021 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
     1081 acattatcaa gctgccgcga aaaaacgcat cccggttatc gaagcgctgt tacgacatcc
     1141 cggtgctttc ctgaagatta ttgcgctacg actgtgcgaa ttgctgacga tgtacatcgt
     1201 tactgccttt gcacttaatt attcaaccca gaatatgggg ctaccgcgcg aacttttcct
     1261 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
     1321 tgccgatcgt tttggtcgcc gtagggttta tatcacaggt gcgttaatcg gaacgttgag
     1381 cgcatttcct ttctttatgg cg
//
LOCUS       DAZOZY010000376         1392 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZOZY010000376 DAZOZY010000000
VERSION     DAZOZY010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.379"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHURQ_004369"
     CDS             <1..459
                     /locus_tag="ACHURQ_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0825961.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHURQ_004370"
     CDS             477..1115
                     /locus_tag="ACHURQ_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR0825962.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          359 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac ac
//
LOCUS       DAZOZY010000377         1384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZOZY010000377 DAZOZY010000000
VERSION     DAZOZY010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.380"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHURQ_004371"
     CDS             complement(<1..1339)
                     /locus_tag="ACHURQ_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR0825963.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZOZY010000378         1377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZOZY010000378 DAZOZY010000000
VERSION     DAZOZY010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.381"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHURQ_004372"
     CDS             <1..984
                     /locus_tag="ACHURQ_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0825964.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHURQ_004373"
     CDS             999..>1377
                     /locus_tag="ACHURQ_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR0825965.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZOZY010000379         1375 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZOZY010000379 DAZOZY010000000
VERSION     DAZOZY010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.382"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..563
                     /locus_tag="ACHURQ_004374"
                     /pseudo
     CDS             1..563
                     /locus_tag="ACHURQ_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
     gene            637..1375
                     /locus_tag="ACHURQ_004375"
                     /pseudo
     CDS             637..1375
                     /locus_tag="ACHURQ_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          354 a          326 c          383 g          312 t
ORIGIN      
        1 aagtatgggt tttaggggat gcggtcgtag atctcttgcc agaatcagac gggcgcctac
       61 tgccttgtcc tggcggcgcg ccagctaacg ttgcggtggg aatcgccaga ttaggcggaa
      121 taagtggatt tataggtcgg gtcggtgatg atccttttgg ggcgttaatg caaagaacgc
      181 tgctaactga gggagtcgat atcacgtatc tgaagcaaga tgaatggcac cggacatcca
      241 cggtgcttgt cgatctgaac gatcaagggg aacgttcatt tacgtttatg gtccgcccca
      301 gtgccgatct ttttttagag acgacagact tgccctgctg gcgacatggc gaatggttac
      361 atctctgttc aattgcgttg tctgccgagc cttcgcgtac cagcgcattt actgcgatga
      421 cggcgatccg gcatgccgga ggttttgtca gcttcgatcc taatattcgt gaagatctat
      481 ggcaagacga gcatttgctc cgcttgtgtt tgcggcaggc gctacaactg gcggatgtcg
      541 tcaagctctc ggaagaagaa tggctgaagt ggcacactga atttggccac ctgaacagag
      601 gtgatatgct cacctcagaa caacacaggt gctccaatga aaaaaagaaa ttttagcgca
      661 gagtttaaac gcgaatccgc tcaactggtt gttgaccaga aatacacggt ggcagatgcc
      721 gccaaagcta tggatgttgg cctttccaca atgacaagat gggtcaaaca actgcgtgat
      781 gagcgtcagg gcaaaacacc aaaagcctct ccgataacac cagaacaaat cgaaatacgt
      841 aagctgagga aaaagctaca acgcattgaa atggagaatg aaatattaaa aaaggctacc
      901 gcgctcttga tgtcagactc cctgaacagt tctcgataat cgggaaactc agagcgcatt
      961 atcctgtggt cacactctgc catgtgttcg gggttcatcg cagcagctac agatactgga
     1021 aaaaccgtcc tgaaaaacca gacggcagac gggctgtatt acgcagtcag gtacttgagc
     1081 tacatggcat cagccacggt tcggccggag caagaagcat cgccacaatg gcaacccgga
     1141 gaggctacca gatgggacgc tggcttgctg gcaggctcat gaaagagctg gggctggtca
     1201 gctgtcagca gccgactcac cggtataaac gtggtggtca tgaacatgtt gctatcccta
     1261 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
     1321 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgtt
//
LOCUS       DAZOZY010000380         1365 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZOZY010000380 DAZOZY010000000
VERSION     DAZOZY010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.383"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..1261)
                     /locus_tag="ACHURQ_004376"
     CDS             complement(<1..1261)
                     /locus_tag="ACHURQ_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR0825966.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          380 a          348 c          305 g          332 t
ORIGIN      
        1 aggtgtaacc tattgcgttg tttgtattac ggtattcggc aacgacttta atcattccct
       61 ccatcatact tgccacttct gtttcctgcg gcgagatcat gcggacattt ttcatgacct
      121 gggattgcat caccgtctgg ctaccagagt cttccgggcg ttgccaggtc tggatctcct
      181 gatcgttacc gccaacagta cgccagttgg taattgcacc actgaagatg tcacgcacct
      241 gttgttcagt cagggaatta accggattat ccgcattgac gatgaaaaca aacgcttcac
      301 gggcaaaagg cgtgtacagc aaagtaacgc ccgattcctc agcgcgtttt ttctgcccac
      361 cggaaggttg cgccacaaaa ataatatcag catcaccttt aacaatcctg ttatacgcat
      421 cgggggtacg agagctctcc agatattccc tggtgtgaaa atcttccggt ataacgctta
      481 aagcataaaa tgcagaggca taaattgggt acgccgccgt agcaccatcg agtcgcggcc
      541 agttttgcgt aaactgaatt tgcggtttac cacgcagtgg tgtcagttga ttattgagtt
      601 tatcgggtcg ccaggcccag gtatcaatat tttcgcgcac accgacaccg ggattgagct
      661 gtttatataa ataaccctga taaccagcga caaaaccaag catgagaagg ataaacagga
      721 gggaattacg gcaacgtagc ccggatgtac cggttaaagg atgtctggtt ggccggccgt
      781 accccagcga aaaactgata tgcgcgccaa cgggtataat gactatccac cagtactcac
      841 ctccgactac tgagactaac aaaacgccca gccaggggag cataagatca gccaaccaac
      901 ccgatcccat ctggtgaaat tgataatctg ccagatgcag cgcgattatc cagccaacca
      961 ggattaataa accgggtact aaaaaaagac attgtcgcca gacggctatt tctctttttt
     1021 ctggtgcact atcgcctgcc catactcgcc ccgagaaaaa tagcattaat gcgcccagcg
     1081 ccagcataaa aatgttgagc agaaggtaac ttgttggcgt gataaggata ttacaaactt
     1141 caataatacc ggcaagtccg acacccagca tagcaacaac cgccgccaaa aattgcgtca
     1201 gtatggggat gccgaaaaaa gtcattacca gcgaggacaa aatccatttt ctgttttgca
     1261 ttattctttc cattcttttt gaatggtgaa attatactcc ccgagtcccc ttgccccttc
     1321 tggacacttt tccgaaatga tggcggaaaa aaacgggacc ctttg
//
LOCUS       DAZOZY010000381         1345 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZOZY010000381 DAZOZY010000000
VERSION     DAZOZY010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.384"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHURQ_004377"
     CDS             complement(<1..185)
                     /locus_tag="ACHURQ_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0825967.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHURQ_004378"
     CDS             complement(230..1216)
                     /locus_tag="ACHURQ_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR0825968.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT          373 a          296 c          271 g          405 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt aacctgtctg
     1261 acaacttgtc tatgtttatc acaacttgaa ataaagtcag aaattaaata ttaaatttgt
     1321 gcgccaggtt gtacatgttt ataat
//
LOCUS       DAZOZY010000382         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZOZY010000382 DAZOZY010000000
VERSION     DAZOZY010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.385"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHURQ_004379"
     CDS             <1..184
                     /locus_tag="ACHURQ_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825969.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHURQ_004380"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHURQ_004380"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR0825970.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZOZY010000383         1334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZOZY010000383 DAZOZY010000000
VERSION     DAZOZY010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.386"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            375..830
                     /gene="csgB"
                     /locus_tag="ACHURQ_004381"
     CDS             375..830
                     /gene="csgB"
                     /locus_tag="ACHURQ_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR0825971.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            871..1323
                     /gene="csgA"
                     /locus_tag="ACHURQ_004382"
     CDS             871..1323
                     /gene="csgA"
                     /locus_tag="ACHURQ_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR0825972.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          397 a          263 c          318 g          356 t
ORIGIN      
        1 ttgttttttc tccacacctc cgtggacaaa tttttactcc aaaaagacga ggtttgtcac
       61 ggcttgtgcg caagacatat cgcagcaatc agcgatgggc aagaagaatg actgtctggt
      121 gatttttgat agcggaaacg gagatttaaa agaaaacaaa atattttttg cgtagataac
      181 agcgtattta cgtggctttt aatactttgg tatgaacaaa aaaagaaaaa tacaacgcgc
      241 gggtgagtta ttaaaaatat ttccgcagac atactttcca tcgtaacgca gcgttaacaa
      301 aatacaggtt gcgttaacaa ccaagttgaa atgatttaat ttcttaaatg tacgaccagg
      361 tccagggtga caacatgaaa aacaaattgt tatttatgat gttaacaata ctgggtgcgc
      421 ctgggattgc agccgcagca ggttatgatt tagctaattc agaatataac ttcgcggtaa
      481 atgaattgag taagtcttca tttaatcagg cagccataat tggtcaagct gggactaata
      541 atagtactca gttacggcag ggaggctcaa aacttttggc ggttgttgcg caagaaggta
      601 gtagcaaccg ggcaaagatt gaccagacag gagattataa ccttgcatat attgatcagg
      661 cgggcagtgc caacgatgcc agtatttcgc aaggtgctta tggtaatact gcgatgatta
      721 tccagaaagg ttctggtaat aaagcaaata ttacacagta tggtactcaa aaaacggcaa
      781 ttgtagtgca gagacagtcg caaatggcta ttcgcgtgac acaacgttaa tttccattcg
      841 acttttaaat caatccgatg ggggttttac atgaaacttt taaaagtagc agcaattgca
      901 gcaatcgtat tctccggtag cgctctggca ggtgttgttc ctcagtacgg cggcggtaac
      961 catggtggtg gcggtaataa tagcggccca aattctgagc tgaacattta ccagtacggt
     1021 ggcggtaact ctgcacttgc tctgcaaact gatgcccgta actctgactt gactattacc
     1081 cagcatggcg gcggtaatgg tgcagatgtt ggtcagggct cggatgacag ctcaatcgat
     1141 ctgacccaac gtggcttcgg taacagcgct actcttgatc agtggaacgg caaaaattct
     1201 gaaatgacgg ttaaacagtt cggtggtggc aacggtgctg cagttgacca gactgcatct
     1261 aactcctccg tcaacgtgac tcaggttggc tttggtaaca acgcgaccgc tcatcagtac
     1321 taatacatca tttg
//
LOCUS       DAZOZY010000384         1330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZOZY010000384 DAZOZY010000000
VERSION     DAZOZY010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.387"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..94)
                     /gene="yqcG"
                     /locus_tag="ACHURQ_004383"
     CDS             complement(<1..94)
                     /gene="yqcG"
                     /locus_tag="ACHURQ_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFR0825973.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDN"
     gene            233..904
                     /gene="queE"
                     /locus_tag="ACHURQ_004384"
     CDS             233..904
                     /gene="queE"
                     /locus_tag="ACHURQ_004384"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR0825974.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          375 a          259 c          290 g          406 t
ORIGIN      
        1 cgttatcatt aaaaactaat acagaaaaaa taaatataat tttggtgaat gttttgacat
       61 gattaaatcc attttcttta ttttcttctg acataatacg ttactccctt taattattaa
      121 cgatatgttt cgcatttttc tttaaatctg cctgtaacag aatgttaata agacatgatt
      181 gttgtggcag gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta
      241 cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc
      301 catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac
      361 ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga
      421 gagtgataag tggggggcgg cgagcagtga agacttgttg gctgtcatta gtcgccaggg
      481 atacaccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg atttgctacc
      541 actgactgat ctgctcgaaa agaacggttt tagctgccag atcgaaacca gtggtactca
      601 tgaggtacgc tgcacaccga atacctgggt taccgtatcg ccaaagctga acatgcgcgg
      661 cggctatgac gtgttgtcac aggcactgga gcgagccaac gaaatcaagc atccagtggg
      721 gcgcgtacgc gatattgaag cactggatga actattggca acgctgaccg atgataaacc
      781 gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt tgtgcattga
      841 aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc taaatattgc
      901 ctgattaaac atttataagc gttataaatg ggtggaacct gtaaagactt ctactcattt
      961 atattgtttg tcgcctctga aaactcctcc attttaccca tccagggcta atcattagca
     1021 ttctctacaa attctgtggc attaattttt cgctggagtg aaaattattg cggtaaagtt
     1081 tggtagattt taatttgtat agagttattt taaatattta cctttttaat caatgaatta
     1141 agtactcttt aacataatgg atgtgttgtt tgtgtgatac tgtaaagttg gtagattgtg
     1201 actggcttaa aaaatcatta attaataata ggttatgttt agagtgttcc ccgcgccagc
     1261 ggggataaac caccatataa cccgttatct ctttctcaag tttttatatt agcagtactt
     1321 gtaataagca
//
LOCUS       DAZOZY010000385         1320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZOZY010000385 DAZOZY010000000
VERSION     DAZOZY010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.388"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHURQ_004385"
     CDS             complement(<1..346)
                     /locus_tag="ACHURQ_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825975.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERA"
     gene            complement(349..1059)
                     /locus_tag="ACHURQ_004386"
     CDS             complement(349..1059)
                     /locus_tag="ACHURQ_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825976.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1075..>1320)
                     /locus_tag="ACHURQ_004387"
     CDS             complement(1075..>1320)
                     /locus_tag="ACHURQ_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0825977.1"
                     /translation="FRYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVT
                     RENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          351 a          317 c          293 g          359 t
ORIGIN      
        1 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
       61 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      121 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      181 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      241 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
      301 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
      361 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
      421 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
      481 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
      541 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
      601 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
      661 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
      721 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
      781 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
      841 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
      901 accgacgaac ttttcccctt catccaaggc ctggttaata agttgctgaa caatgccttc
      961 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1021 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1081 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1141 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1201 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1261 gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa
//
LOCUS       DAZOZY010000386         1299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZOZY010000386 DAZOZY010000000
VERSION     DAZOZY010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.389"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHURQ_004388"
     CDS             <1..1117
                     /locus_tag="ACHURQ_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0825978.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..>1299
                     /locus_tag="ACHURQ_004389"
     CDS             1095..>1299
                     /locus_tag="ACHURQ_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825979.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          356 a          263 c          400 g          280 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaag
//
LOCUS       DAZOZY010000387         1290 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZOZY010000387 DAZOZY010000000
VERSION     DAZOZY010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.390"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(128..502)
                     /locus_tag="ACHURQ_004390"
     CDS             complement(128..502)
                     /locus_tag="ACHURQ_004390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825980.1"
                     /translation="MRKKENKAKKEKFRQFFPENYDIGISLNEENQLRLFSKRNDSQD
                     ESLCKYEFSNKIKVQYIFLPYSSEIQVITDEFPLTEAGCQECTQAFKHPISMELIEEI
                     KEAKCSVTGLVIYSKPILKLIS"
     gene            complement(825..>1290)
                     /locus_tag="ACHURQ_004391"
     CDS             complement(825..>1290)
                     /locus_tag="ACHURQ_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFR0825981.1"
                     /translation="SEILNSKNIKEIPVFDFDINKVLDPLNVELRAKLRKEVRLVLKS
                     VKYWVFEKRIFVQLEYFNDVLSHTLVIDNKRGGGEVLHEIVISETSGERVYTVNEEGK
                     TVFIASVTSGTDMWSLAISRTKILDDVGLYLNLLKREKFEIIVNEDMIDWMD"
BASE COUNT          395 a          256 c          193 g          446 t
ORIGIN      
        1 gtaaagtaca aagcctacta tctcgtaaag taatttagta aaccattcca ttttgaagta
       61 cccccattca cattcaactt tatagtattt agtgagggag tacttaaaac gtaacaattt
      121 ggatgtgtta gctaataagt tttagtatag gtttcgaata aatgacaagt ccagtaactg
      181 aacattttgc ttcttttatc tcttcaatta gttccattga tattggatgt ttgaaagctt
      241 gagtacactc ctgacagcct gcctccgtta gcggaaactc atctgttata acttgaattt
      301 cagaagaata cggcaaaaaa atgtactgaa ctttaatttt gttggaaaat tcgtacttgc
      361 ataagctctc atcttgagaa tcattccgct ttgaaaaaag ccttaattga ttctcttcat
      421 tcaatgagat cccaatatca taattctcag ggaaaaattg acggaatttt tctttttttg
      481 ctttattttc tttcttacgc attttggcaa tcctctcttt agaattcatc tcatggtttc
      541 atcgctccta ttcgttagtg aatgacgcta tggtactggt ttaacgttga aaagtcaaaa
      601 aagttaattt tacccttgac tattctaaca cacgctgata taaatgattt gctgtatttt
      661 gcacggaaat cttaaaccca tcgggtgcgt agttatatcc aaaattcata atccctcttt
      721 tattgctcta tctacaatgc cgttagattt cgacatcgta gatatattgt tcaaatatta
      781 actcgcataa ttcattctac aagaccataa attctcatta gcttttaatc catccaatca
      841 atcatatctt cattaacaat gatctcaaat ttttcacgct tgagaagatt aagatacaat
      901 ccaacatcat caagtatttt ggtacggcta atggcaagcg accacatatc agtaccagat
      961 gtaacggagg caatgaaaac ggttttgccc tcttcattga cagtgtagac acgctcgccg
     1021 cttgtttcag agattacgat ctcatgtagt acttcacccc cgccccgctt gttatcaatc
     1081 acaagcgtat gggataggac atcattgaag tattccaact gtacaaatat acgcttttca
     1141 aaaacccagt acttaacaga tttcagtact aatcttactt ctttccgtaa ctttgcacgt
     1201 aactcgacat tcaatggatc aagtacttta ttaatatcga aatcaaatac agggatttct
     1261 ttgatgttct ttgaatttaa tatctcagat
//
LOCUS       DAZOZY010000388         1287 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZOZY010000388 DAZOZY010000000
VERSION     DAZOZY010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.391"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHURQ_004392"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHURQ_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
BASE COUNT          374 a          269 c          276 g          368 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtt
//
LOCUS       DAZOZY010000389         1283 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZOZY010000389 DAZOZY010000000
VERSION     DAZOZY010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.392"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(246..818)
                     /locus_tag="ACHURQ_004393"
                     /pseudo
     CDS             complement(246..818)
                     /locus_tag="ACHURQ_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(830..>1283)
                     /locus_tag="ACHURQ_004394"
     CDS             complement(830..>1283)
                     /locus_tag="ACHURQ_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0825982.1"
                     /translation="CCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEW
                     LTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDG
                     LTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
BASE COUNT          352 a          349 c          281 g          301 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaatactg gatttgcccc tatatttcca
      841 gacatctgtt atcacttaac ccattacaag cccgctgccg cagatattcc cgtggcgagc
      901 gataacccag cgcactatgc ggatgccatt cgttataatg ctcgaacgcc tctgcaaggt
      961 tctttgctgc cgttaacccg tctggtttgg gcatgatact gatgtagtca cgctttatcg
     1021 ttttcacgaa gctctctgct attccgttac tctccggact ccgcaccgcc gtgttcttcg
     1081 gttcaagtcc caacatccgg gcgaactggc gtgtttcatt agcccggtag catgaaccat
     1141 tatccgtcag ccactccact ggagacgacg gaagatcgtt gccgaagcgg cgttccaccg
     1201 ctcccagcat gacgtcctgt actgtttcac tgttgaagcc gccggtagtc accgcccagt
     1261 gcagtgcctc acgatcacag cag
//
LOCUS       DAZOZY010000390         1282 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZOZY010000390 DAZOZY010000000
VERSION     DAZOZY010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.393"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            132..1151
                     /locus_tag="ACHURQ_004395"
     CDS             132..1151
                     /locus_tag="ACHURQ_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR0825983.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAZOZY010000391         1264 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZOZY010000391 DAZOZY010000000
VERSION     DAZOZY010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.394"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            233..751
                     /locus_tag="ACHURQ_004396"
     CDS             233..751
                     /locus_tag="ACHURQ_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR0825984.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            961..>1264
                     /locus_tag="ACHURQ_004397"
     CDS             961..>1264
                     /locus_tag="ACHURQ_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0825985.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          310 a          311 c          324 g          319 t
ORIGIN      
        1 ctttgaacag ttgggtatgg ttatccagat atatattatt aagttattgc tgaataagag
       61 atttaatgat tttaaaaata gtttttacga caaccgtaaa ttctagctcg gtttgtatgt
      121 aaaaaatact gaaaaatgat gcggttccgt gaagtgagct gcatctgcct gtctgggatt
      181 aacggcaggt cattgtaagt agtaattaac cacgttaaca aggagtttac aaatgccaac
      241 accgtgttat atctccatca ctggtcagac ccagggaaat attactgcag gtgcatttac
      301 cgctgattct gtcggcaaca tctatgtgca ggggcacgaa gacgagatgc tggtgcagga
      361 attcctgcat aacgttactg tcccgaccac tcctcagtcc ggtcagcctg cgggtcagcg
      421 tgctcacaag cctttcattt ttaccgtggc gctgaacaaa gcggttccgc tgatgtacaa
      481 cgcactggcc tccggcgaaa tgctgccaac cactgaactt cactggtggc gtacctctgt
      541 ggagggcaaa caggagcatt atttcactac ccgcctgact gattcgacga ttgtcgatat
      601 gaaactgcat atgccgcatt gccaggaccc ggcgaaacgc gagttcaccc agttgctcga
      661 ggtttcactg gcctaccgca aaattgaatg ggaacacgtc aaatctggca cctccggtgc
      721 cgacgactgg cgcgcaccac tggaagcata agttaagcca acagcatccg gctatcaggc
      781 cggatgctgt ttttgtatta cgccgtagaa agaaaggttg tcagtaaggg gttacgttca
      841 cgggagatgg cggatgccgg aaacttcggc ggtagcgtgc ctgaacgcaa gcgtaacact
      901 gtactgtcac agccgcttta acagcgaact gtctgttttt ccttaacccc ggaggttgct
      961 atgtcaaccg gattacgttt cacgctggaa gtggacggcc tgccaccgga tgcttttgcg
     1021 gtggtctcct ttcatctgaa ccagtcactc tcttcgctct tttccctcga gctctctctg
     1081 gtcagccagc agtttctctc ccttgaattt ccgcaggtac tggacaaaat ggcctacctg
     1141 acgatatggc agggcgatga cgtacagcgc cgggtgaaag gtgtggtgac ctggtttgaa
     1201 ctgggggaga acgacaaaaa ccagatgctg tacagcatga aggtatgccc accgctgtgg
     1261 cgca
//
LOCUS       DAZOZY010000392         1260 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZOZY010000392 DAZOZY010000000
VERSION     DAZOZY010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.395"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            99..701
                     /gene="ais"
                     /locus_tag="ACHURQ_004398"
     CDS             99..701
                     /gene="ais"
                     /locus_tag="ACHURQ_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR0825986.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(740..1165)
                     /gene="nudI"
                     /locus_tag="ACHURQ_004399"
     CDS             complement(740..1165)
                     /gene="nudI"
                     /locus_tag="ACHURQ_004399"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR0825987.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          351 a          283 c          266 g          360 t
ORIGIN      
        1 aggattttta atattacacc aactgctaat tatcgccaga aatgtcgctc aatttcacgg
       61 taattgtctg gttgcgcttg tctataggtg gagtttacgt gttagctttt tgccgctcat
      121 cattgaagtc aaaaaaatat ttcatcattt tactggcgct cgctgcaatt gccggactgg
      181 gtactcatgc cgcctggagt agcaatggtt tgccacgtat cgacaataaa acattggcca
      241 gactggcaca gcagcacccg gttgtcgttt tgtttcgcca tgctgaacgt tgcgatcgtt
      301 caaccaatca atgcttgtca gataaaacag gtattacggt taaaggtacc caggatgccc
      361 gtgaactggg caacgctttt agcgctgaca tccctgattt cgatctttat tccagtaata
      421 ccgtccggac cattcagtcg gcgacctggt tttccgcggg taaaaaactg acggtagata
      481 aacgacttct tcagtgcggt aatgagattt atagtgcaat taaggactta caaagcaaag
      541 cgcctgataa aaatatcgtt attttcaccc ataatcattg cctgacatat attgctaaaa
      601 ataagcgtga tgcgacattt aaacctgatt atctggatgg tttagtcatg catgtggaaa
      661 aaggcaaagt ttatctggat ggggaattcg ttaaccacta aattttcaat ctgacagcca
      721 gtgatggctg tcatcattgt taaagaagac ctttcaaacg taacgttttt cgggtggcaa
      781 catttaaatc ataatgcacc agatcttcag gttttaccca cgcgtagtcc tgaaactctt
      841 cgtttatttt cacttctcgg ttggcagaaa cgcagtcaaa aatcaggtaa atcatataaa
      901 tctcttcctt gcgaccatct gcatacgtct tggtgcgaat atcatcgctg aaggtccacg
      961 gcgtgatttc tgtcaaaagc agctgttctc ccagttcttc gcgaatttcg cggcgtagtg
     1021 cctcttcaat tcgttcgcca gactccacgc cgccacccga aagcgcccat tgaccgggga
     1081 aaacgccgcg atcgtcggcc attttgcaca gcaaataagc accatcattt tgaatcaaag
     1141 ggcatacaat agtccgttgt cgcacagggg tattccttat aaataaaaag aaaccatgag
     1201 aatacggtaa ctcagaaata tgtgaaagtg acagtaatct cttttgcgat gataactgca
//
LOCUS       DAZOZY010000393         1231 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZOZY010000393 DAZOZY010000000
VERSION     DAZOZY010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.396"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..1231
                     /gene="rhsB"
                     /locus_tag="ACHURQ_004400"
                     /pseudo
     CDS             1..1231
                     /gene="rhsB"
                     /locus_tag="ACHURQ_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
BASE COUNT          300 a          356 c          380 g          195 t
ORIGIN      
        1 cgctggtgcg ctatggctgg acgccacgcg gcgaactggc ggtggtgtat gaccgtagtg
       61 gcaaacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg gtggcgcacc
      121 gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg gtgacagaac
      181 agcttaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc atcaccatca
      241 ccgacagcct gaaccgccgt gaagtgctgc acacgtaggg tgaagccggg ctgaagcggg
      301 tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgac gccgtgggca
      361 ggctcaggac acagacggat gccgcaggcc ggacaacaga atacagcccg gatgtggtga
      421 cgggcctcat cacgcgcatc accacgccgg atggcagggc atcggcgttt tactataacc
      481 accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc cgcaaatatg
      541 atgaatccgg ccgtctgatt caggaaaccg cccctgacgg cgatatcacc cgctaccgtt
      601 atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc agccggaaaa
      661 ccatgacatg gagccgttac ggtcagttgc tgaccgtcac tgactgttcc ggttatgtaa
      721 cccgttatga ccatgaccgt tttggtcagg tgacggcggt gcaccgcgag gaagggctga
      781 gccagtaccg cgcatacgac agccgtggac agttaattgc ggtgaaagac acgcagggcc
      841 atgaaatgcg gtatgaatac aacgccgccg gtgacctgac cgccgtcatt gccccggacg
      901 gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc catctgtacc acgcagggcg
      961 ggctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgtctg accagtgaaa
     1021 acggcagcca caccaccttc cgttacgatg tactcgaccg gctgatacag gaaaccggct
     1081 ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc cgcagcgagg
     1141 atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg caccgcaccg
     1201 tgaagggtga aaccgcagag cggtggcggt a
//
LOCUS       DAZOZY010000394         1188 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZOZY010000394 DAZOZY010000000
VERSION     DAZOZY010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.397"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(849..>1188)
                     /locus_tag="ACHURQ_004401"
     CDS             complement(849..>1188)
                     /locus_tag="ACHURQ_004401"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825988.1"
                     /translation="LMARLREVLNAIFSAPRSGKSRIPKYQQGIFLRNQHRFYLLNHY
                     TESLSVMSLTKLSLNTRMIGFMSDQMLETAPRLTRAVSDETSVYAGAGQNIGQNPLNI
                     INVICAKEHI"
BASE COUNT          346 a          243 c          198 g          401 t
ORIGIN      
        1 tatttatact tatcgggtat cagttttgat aggggtatca gacttgatag gggtaacaga
       61 attctgtggg ggtagttcac tatgacctac ccctagttca ctgtgagcta ccgttacatc
      121 caatcatctt ccgatgtaac gtatcgttac taccgatgtg taatatcgtt acattcaata
      181 attagtctat gtaacatatc gttactacca actttcttac agagagcatt tcagataaat
      241 gaatgtaacc tctcgttaca tccatttgtc acatagcact ctgtgtaatg tctttctatg
      301 tgtgtgccat aatcccccta tattccctgt gtaaatcata gaataatgct ctctattccc
      361 tgtgtaattc cctactaaaa aatccaatga aaagtagctt aaaagttcaa ttcttacttt
      421 cttcactttt acatcacttt cgcaaagcct tgtgtaatgc ggcttacaga gcaccatcag
      481 aaattgttgg ctaaaaattg ttctgcttgt ttctgaacga gtttctcgtt tgtgcaaatt
      541 ttaaaattta ttttgttttt atagccttat ttcgtgtaat gcctggcata gcaagccctt
      601 ttcttgtgtt gtcaagacat ttggcaacgt tcaaacatct gtttagaaat gccaaattgt
      661 tgatctaaca aataaacctg tataaacaga acaccattta atctattccc ctgtgtaacc
      721 caccacatca cacaataaaa acaaaaaact ttaattatat ttcatagcat acaaaagtca
      781 atagaagccc tttcgtgctc ctacgtaaaa aatccatcac ttttcccctg atacatcaat
      841 tcaaggcgtt atatgtgttc cttagcacat ataacattta ttatgttaag tggattttgg
      901 cctatatttt gacctgcacc cgcataaaca ctggtttcat cgcttacagc ccttgtgaga
      961 cgtggtgctg tttctagcat ctgatccgac ataaaaccga tcattctcgt gtttagtgaa
     1021 agtttagtga gagacattac agaaaggctt tctgtgtaat gatttaatag gtaaaaacga
     1081 tgttgattac gcaagaaaat tccttgctga tatttgggga ttctggattt tccagatcgg
     1141 ggggcgctga aaatcgcgtt aagcacctct cgcaatctag ccataagg
//
LOCUS       DAZOZY010000395         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZOZY010000395 DAZOZY010000000
VERSION     DAZOZY010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.398"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHURQ_004402"
     CDS             complement(<1..755)
                     /locus_tag="ACHURQ_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR0825989.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHURQ_004403"
     CDS             1071..>1187
                     /locus_tag="ACHURQ_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825990.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZOZY010000396         1179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZOZY010000396 DAZOZY010000000
VERSION     DAZOZY010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.399"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..118
                     /gene="rcbA"
                     /locus_tag="ACHURQ_004404"
     CDS             <1..118
                     /gene="rcbA"
                     /locus_tag="ACHURQ_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFR0825991.1"
                     /translation="KLTKSECEKMLSGEKEAGVSREQKVKLINFNCEKLQSS"
     gene            218..433
                     /gene="xisR"
                     /locus_tag="ACHURQ_004405"
     CDS             218..433
                     /gene="xisR"
                     /locus_tag="ACHURQ_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR0825992.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            435..>1179
                     /locus_tag="ACHURQ_004406"
     CDS             435..>1179
                     /locus_tag="ACHURQ_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR0825993.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          408 a          213 c          271 g          287 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgtttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 tgcattgtat tcaaattaaa acttcatagc tgattattaa taatcaacat cgggcgtcaa
      181 tttcagccta acattggcgc ctgccagagg tgatgcgatg gcacaagtaa tctttaatga
      241 agagtggatg gttgaatacg gcctgatgct tcgcactggt ctgggtgcca gacaaattga
      301 agcataccgc cagaactgtt gggtggaagg cttccacttc aaacgagtat ctcctttagg
      361 gaagccagac agtaagcgag ggattatctg gtacaactat ccgaagataa atcagtttat
      421 caaagactca tgatatgtct aaattaccaa caggtgtcga gattcgaggt aaatacattc
      481 gcatctggtt catgtttcga ggaaaacgat gtcgggaaac attgaaaggc tgggagatta
      541 ctaacagtaa cattaaaaag gccgggaatt taagagcgtt gatagttcat gaaatcagtt
      601 ccggtgaatt tgagtattta agacgttttc cccagtccag cactggggca aaaatggtga
      661 caacgagggt cataaaaaca ttcggggagc tttgtgatat ctggacaaaa attaaagaga
      721 cagagttaac aacaaacaca atgaagaaaa cgaaatcaca attaaaaaca ctcaggataa
      781 taatttgtga gagtaccccg atatcgcata ttcgttatag cgatatctta aactaccgga
      841 atgaactgct gcatggagaa acgctttacc tagataatcc aagatccaac aaaaaaggaa
      901 gaaccgtgcg cacagttgat aactatatcg ccctgctctg ttcgttgtta cgttttgcgt
      961 atcagtcggg atttatatca accaaaccat ttgaaggagt aaaaaaatta cagcgaaaca
     1021 gaataaagcc tgacccgtta tctaaaacag aattcaatgc attaatggaa agtgaaaaag
     1081 gacagagcca aaacttgtgg aaatttgccg tttactccgg gcttcgtcac ggggaactgg
     1141 cagctctggc gtgggaggat gtggatttcg agaagggaa
//
LOCUS       DAZOZY010000397         1176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZOZY010000397 DAZOZY010000000
VERSION     DAZOZY010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.400"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>1176)
                     /locus_tag="ACHURQ_004407"
     CDS             complement(<1..>1176)
                     /locus_tag="ACHURQ_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFR0825994.1"
                     /translation="PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLA
                     RGGVAAQHSSQPLSALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVV
                     VDGFGRSLTFHRAAEGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSP
                     ASPRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAG
                     GELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLD
                     YRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQT
                     DAAGRRTEYRLHMASGKLTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDRQG
                     "
BASE COUNT          199 a          430 c          326 g          221 t
ORIGIN      
        1 ccctgacggt catatttccg gctgctgcgt aaaccatccg gatatgtcac tgacgtcagt
       61 tgcaactggt tgttgtaacc ataccgtacc gtcctgccgt ccggcagtac cactgaagtc
      121 agctttccag acgccatatg cagccggtac tccgtccgcc gccccgccgc atccgtctgc
      181 gccttcagcc tccccgcctc atcatactcg ctgcgggtga tgctcccgtc ggcatgctcc
      241 ttcttcacca cccgcttcag gccaccctcg ccttccgtgt acagcacctc ccgccggttc
      301 aggctgtccg tgatgattac ccggctctct ccgtactcaa agcggtagtc cagcccctcc
      361 gggttgacct gctccgtcac ccgaccggta tcatcatacc ggtagcggct ctccggcctc
      421 cccgcataat ggtgcgccac catccgcccg gcgtgctccg catcataagt aaacccgcga
      481 acctgcgtcc cgctgcggtc atacaccgcc cgcagttcac caccggccgt gtacgtgtag
      541 cgcgccagcg gcgcagtcgg ctgttcatcc gggtatgccg ggtcgtgtgt cagccatacc
      601 gcctccagcc ggataccgtt atcggcaccg tactctgtac cggcgggcag cgtgtcgggg
      661 aaaacctgag aagaggaaac agaacgggga ctggccgggg aagataagga agaggcgcgc
      721 tgtttacggg acgcttccgc ccgctgcgcc tgcgtgctca gcgccatgtg aaaacggcgg
      781 cccgcgccgt ccgtcacccc cgtcaccgcg cccgccacgt caccctccgc cgcccggtgg
      841 aaagtcagtg agcgcccgaa gccatccacc acgaccgtca gtacccggta tgcgggcggc
      901 tccggccagc tgagtatcca ccacggcccc tgaaggctgt ttgtcgccag gtacacatgc
      961 ggactcaggc gaacatcttc cggcagcacc tgccagagcg cgcttagcgg ctgcgaactg
     1021 tgctgcgccg ccactccgcc ccgcgccagc cagagtgact cgctgcggct gtagcttatc
     1081 tcgccgggaa acagcggctc aaagtgaatg ctcctgccgc cgctgtcgtt gagtatcagg
     1141 ccttcatcgc gtatctgtaa gcggatatcg aagggc
//
LOCUS       DAZOZY010000398         1174 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZOZY010000398 DAZOZY010000000
VERSION     DAZOZY010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.401"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            50..733
                     /gene="tssD"
                     /locus_tag="ACHURQ_004408"
                     /pseudo
     CDS             50..733
                     /gene="tssD"
                     /locus_tag="ACHURQ_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            713..>1174
                     /locus_tag="ACHURQ_004409"
     CDS             713..>1174
                     /locus_tag="ACHURQ_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0825995.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          360 a          235 c          257 g          322 t
ORIGIN      
        1 acaatactat ttctctttca caaaagaatt aacaaaaggt ttttcaagga tgagtgatat
       61 tatttacctg accataaccg gagaacaaca aggatgtatt tcctctcgtt gtggaacgag
      121 cgcatcaata ggtaatcgct ggcagattgg ccatgatgat gaaatcttcg cgttctcgct
      181 ctctaatagt attacgaata caggcaaagg gtcataattg catggcctta gtttctgtaa
      241 attgattgac aaaagctccc cgttacttat taacgctatt aacaataacg agcagttgtt
      301 catggagttt gacttctatc ggatcaatag gtttggtcga tgggagaaat attacaacat
      361 tcaactccgc ggcgcgctct tatcagcaat caatcacctg tttactgaga acaatctgga
      421 tacagaagcc atcactgtca gttattaata tatactctcc aggcatctga ttgctaatac
      481 tgaatttagt tattgggcat ttcccgataa ctacaaccgt ctgtttatac cacggccaaa
      541 aactaaagct ggtaatggtt tcaaaacact taatagtaaa gcggttggca gactgcttgc
      601 tgctgtcggc atatacaatg ggaatatcga aggattcagg gaaacagcag acaagttggg
      661 gggagatgca cctgctggtt atgatcaatt tatggataac aaagggttac tcatcgctgg
      721 tgcttcaata gttgctggag cagttctggg gagaatgaga ctccctgatt tggaatcact
      781 tgagcatttc ggagccagag gagcagtatc aggaagacca tttaatcccg agcttgccgg
      841 tggtccaata gaaaacctga cgacagatgg cgtaacaatc aacagggaag gtatcgctat
      901 tgttgaaaaa catatagcca gatttggtca tgatcctgta aatgaagtga tgatcaatag
      961 actgaaagac attgagaaag gaaaaatccc cccagaacaa gtagatctta acttctatac
     1021 tcatgagtgt cgggaatatc agcgttactg taatcttggt tgggaaacag gacaacctga
     1081 tggtgacgca ggctatgcac tctggaatca tactggattt gcccctatat ttccagacat
     1141 ctgttatcac ttaacccatt acaagcccgc tgcc
//
LOCUS       DAZOZY010000399         1170 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZOZY010000399 DAZOZY010000000
VERSION     DAZOZY010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.402"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>1170
                     /gene="fimD"
                     /locus_tag="ACHURQ_004410"
     CDS             <1..>1170
                     /gene="fimD"
                     /locus_tag="ACHURQ_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR0825996.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQM"
BASE COUNT          331 a          261 c          283 g          295 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg
//
LOCUS       DAZOZY010000400         1161 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZOZY010000400 DAZOZY010000000
VERSION     DAZOZY010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.403"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            247..492
                     /gene="yahM"
                     /locus_tag="ACHURQ_004411"
     CDS             247..492
                     /gene="yahM"
                     /locus_tag="ACHURQ_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR0825997.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(509..>1161)
                     /locus_tag="ACHURQ_004412"
     CDS             complement(509..>1161)
                     /locus_tag="ACHURQ_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR0825998.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          274 g          258 t
ORIGIN      
        1 tttcgcagcc atgcggtgta taaaaaatga tctcatgcag atgttttgtg aatgtgtggg
       61 ttgacattca tatgaaaaaa atcataattc catcatgttt gtgtggggtc ttttctgtac
      121 cttacgcatc gcactcaagc ctgacagaaa atatgctgta aggctcatat caaaaaccgc
      181 cattagctca tcaggaagag cagacgacaa ccaggattgt tgtatggtac ggggttcgag
      241 gcctcgatgg cggtccaact tttcaaaact cttctgaatc agatcccctt attatcatcc
      301 ctgcaaagtg gaacattgcc attgtttggc tattcaggac gggtcagacc aatgaaaaaa
      361 gcccacacag gggagagtgg gctgaaatgg gaagctaaag actcaagtaa acttatcgga
      421 aataaggacc acgcattacg ggggctatca tcgccgatag tcgggattcg gcaaatcagg
      481 ttgattacgt agagagtaaa ttatctgctc accgctgcgt caccccttcg taaatcaggc
      541 gtagcgcgaa tacaccaata attgcaccaa taacccgact ggcaacgcgt tgcatacgcc
      601 cataagcacg acgcacagcg ggcaaagaaa acgcctgact aagaaagact cgccagataa
      661 ctgatgcgag cacaatcccc gcccaggcca ttaaacgtgc ccatgttggt gtttcggcat
      721 ttaatgttac tgagaaaata ctgataaaaa ataaaacggt ttgcgggtta gagagatcgg
      781 taattaatcc gcggcgaaaa aagacatacc aggaggcgct aatcggttgt tgtagtgtgc
      841 tcatttgcgg tgttgactgg cggcgcatgc tgcaccacgc aaaccataag agataagcgc
      901 cgccgacgat tctgataagc gaaaaaatct cctcacactg cgttattagc gttgcaagac
      961 caaacaaacc caacccggaa taaaatgcat cgcccagcgc cacgcccagt ccggtcagca
     1021 cccctgcgcg tcgaccggaa gccaggctgg tttgtactac cacaaagaga ttggctcccg
     1081 gattaaaaaa agtaatcacg aacagtccta cggtcaggta aacggcgtgc aaaggatcca
     1141 tagtgatttc atccataaat a
//
LOCUS       DAZOZY010000401         1155 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZOZY010000401 DAZOZY010000000
VERSION     DAZOZY010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.404"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHURQ_004413"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHURQ_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1155
                     /locus_tag="ACHURQ_004414"
     CDS             572..>1155
                     /locus_tag="ACHURQ_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0825999.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          274 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggt
//
LOCUS       DAZOZY010000402         1145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZOZY010000402 DAZOZY010000000
VERSION     DAZOZY010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.405"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHURQ_004415"
     CDS             <1..187
                     /locus_tag="ACHURQ_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR0826000.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHURQ_004416"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHURQ_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR0826001.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          273 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgc
//
LOCUS       DAZOZY010000403         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZOZY010000403 DAZOZY010000000
VERSION     DAZOZY010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.406"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHURQ_004417"
     CDS             <1..>1140
                     /locus_tag="ACHURQ_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR0826002.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          361 g          206 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtactgcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZOZY010000404         1138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZOZY010000404 DAZOZY010000000
VERSION     DAZOZY010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.407"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            38..823
                     /locus_tag="ACHURQ_004418"
     CDS             38..823
                     /locus_tag="ACHURQ_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826003.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(960..>1138)
                     /gene="ykfM"
                     /locus_tag="ACHURQ_004419"
     CDS             complement(960..>1138)
                     /gene="ykfM"
                     /locus_tag="ACHURQ_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YkfM"
                     /protein_id="HFR0826004.1"
                     /translation="LIAIVIAPLIAPFVTGYVNANYHPCGNNTGIFPGAIYIKNGMKC
                     NNEYISRKEDSAVK"
BASE COUNT          372 a          204 c          245 g          317 t
ORIGIN      
        1 attaccatga ttatgacagg cgagggagtg tccgattatg aatttaagaa gggtttgttg
       61 tatatgtgta ttattctcgc tgcttgcagg atgtgcctct gaatcttcta ttgatgaaaa
      121 gaagaaaaaa gctcaagtca cacaaagtaa tattaataaa aacactcccc agcaactgac
      181 agacaaagat ttattcggta atgaaaccac tctggccgta tccgaagagg atattcaagc
      241 tgcgcttgat ggagatgagt tccgcgttcc ccttaattct cctgtaatac ttgttcaatc
      301 cggcaaccgc gcaccggaaa ccattatgca ggaggagatg cgaaaatatt atactgtttc
      361 cacattctct ggtatcccgg acaggcaaaa gcctctgact tgtaacaaaa acaaagataa
      421 aaatgagaac gaggatgttg ccagtgctga gaatatgaac tggatgcagg cactgcgttt
      481 tgtggctgca aaagggcatc agaaagcgat tattgttttc caggatacgt tgcagacagg
      541 aaaatatgac tctggattga aatcaatagt ttggtcagat tataaaaatc aaaaattgac
      601 tgacacgatg tcactgcgct atctggtacg attcacgctg gtggatgtag ctacaggtga
      661 gtgggctgcc tggtcgccgg tgaattatga atataaagtg cttccaccat tgcccgacaa
      721 gaatgaagcc agtacgactg atatgacaga gcagcaaatc atgcaactta agcaaaagac
      781 ctataaagcg atggtaaaag atttggttaa ccgctatcaa taataaatta tatctgccgc
      841 caggaatttc tggcggaaat aatacaaaat tctttgcata agaaattttt cttgacaaag
      901 acagtgcaag aaaggacatg gaatccggag ttaaaaactg tttgttttag aatgtttaat
      961 tatttaactg ctgaatcttc cttgcgagaa atatattcat tattacattt catgccattt
     1021 ttaatataga ttgctcctgg gaaaattccc gtattattac cgcaggggtg ataattagca
     1081 ttgacatatc ctgtgacaaa aggagctatt aaaggtgcta ttacgatagc tattagta
//
LOCUS       DAZOZY010000405         1137 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZOZY010000405 DAZOZY010000000
VERSION     DAZOZY010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.408"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..153
                     /locus_tag="ACHURQ_004420"
     CDS             <1..153
                     /locus_tag="ACHURQ_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0826005.1"
                     /translation="AAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVL
                     AGSGLS"
     gene            164..601
                     /locus_tag="ACHURQ_004421"
                     /pseudo
     CDS             164..601
                     /locus_tag="ACHURQ_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            601..978
                     /locus_tag="ACHURQ_004422"
     CDS             601..978
                     /locus_tag="ACHURQ_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826006.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          366 a          168 c          301 g          302 t
ORIGIN      
        1 gccgcagaat tattttcagg gatacggcac atcgctatta atattttaac gaatgataag
       61 gtattcaagg cagggttaag acgtaagatg cgaaaagcag ccatggatag aaactatctc
      121 gcgtcagtcc ttgcggggag cgggctttcg taatctttcc ctagggagtc agccgaatgt
      181 tgggaaggat ttgacggatg aagagaagaa ctgcttggcc ggcggcagta cgggaagccc
      241 gggaggatgg ggaccagaag atgagaatag cgcaagggat cgtatgcagc atgaacggta
      301 taaagatgaa ttgcgacaga aaatgggaaa acccgacgtt acagattcaa atttgaaaaa
      361 tattatagac gatctttatc ggccaaatgc aaaagtagga agtggaagca cagcagatgc
      421 tgttcgttat gaattggcca caggtgaaaa agttggtggt agaggacatg tattgaaagc
      481 acaggattat acaagggctt tacaggattg gttggataag aatccaacag catctccagg
      541 ggatcgagca gcagcagaaa acgttcttca ggatttacag aatgctttgg gaggcaagta
      601 atgaaccaat atattgctag ttatatagaa aaaatggcat cattttccac tgttttgtct
      661 gagacagtct cattagctaa tgaatactgg catccagaaa cgccaccggt ggttatgttg
      721 ttctctcagg ttggaaaaac gattgtaaaa aatttttatg aaatggggga tattgaaaga
      781 gaaaaaatat ttaaacatgt cgaagatggt atggtctcgc ttgatgatga attggccaca
      841 gttgtagcta cagggcttat agaggcaata gtcactgcga ccgatagcaa ttctttactt
      901 tggcaagatt taagtaaatt tctgggtgac aaatcaaagt ctcatgcatt agtgtggaaa
      961 aattttggtc aggagtgata ttattcaaga agtattaagc tcgctattga gatcttaatg
     1021 cttcttgaga ttaaattaaa gttaggacga agtaattgca ggtggaaata aatccatagt
     1081 gacgtgtggc aatggctatt agatgttatg gtgtgatggg ctttggtgat ttaagag
//
LOCUS       DAZOZY010000406         1122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZOZY010000406 DAZOZY010000000
VERSION     DAZOZY010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.409"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(32..>1122)
                     /locus_tag="ACHURQ_004423"
     CDS             complement(32..>1122)
                     /locus_tag="ACHURQ_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR0826007.1"
                     /translation="TITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPME
                     TGKIALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRV
                     ALSASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAIL
                     VFPFFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYS
                     HDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLH
                     NDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSK
                     PVPTAVFVTIILLCAYFKAQSDQCLLDK"
BASE COUNT          392 a          183 c          199 g          348 t
ORIGIN      
        1 gcctccttgc tgattttatt tcacatattc attatttatc taataaacat tggtctgatt
       61 gtgctttaaa ataagcacaa agcaatatta tggtgacaaa cacagcagtc ggaaccggtt
      121 tagaaaagag tattacatca cttaggccta ctaccagtag tgaaaaaagc aagattaaaa
      181 tataaggttc tttagcagtt cttaaggcat atatgagtat tgctgaaaag gctaaaattg
      241 ttaatacaac tccaggaata ccacgcgttg ataacgtatc tatgagatcg ttatgcaaat
      301 gagaatctat atagggtaaa gcgccactca atctaggctc tttttcttct agttcatgta
      361 ttttttccgc tcgtttttcc agtgattgtc ctattggaga atatgttttt aagccaactt
      421 catacattgc cagacgggca ccgactgaag ttctggtgtt atcatgcgaa taattaacta
      481 aatcgttatt taagtcattc attctcataa gcaatgtatc tttaaaagaa aatacacctg
      541 ctaataatgc aatcgcaata aaacaatata acttataatt aattcgctta ttaatataac
      601 tatccattac gattaatagt aacgcaaaga atggaaacac taatatggct gcacgcgttt
      661 cagttactat gacagcacat aagctaagca tgaagttaat tgtatataat gttgttttat
      721 gtctaaagtc ggattttaaa atgagaattg atgccagtaa agaagggaaa aggatgatgt
      781 agcctgctgt tgtagcgtgg gaagctgata atgcaacacg gtcaacgttt aaatagaaat
      841 gttgccacat ggcatgcgca aaaaaaagta actgcgttgc cagggatgcg aacagaatta
      901 gtttcccaaa ttttattttt gttctcaact catttttagc aaaaagaacc aagaaaataa
      961 aagcactaca caaggcaatt ttcccagttt ccattgggcc acgatatgca ttggtataaa
     1021 cagagcctga aactttataa tgtgaatacc agatgatatt tatgattccc aaaagacata
     1081 atgagatgaa aattaacaaa gtaggattgt tagttattgt tt
//
LOCUS       DAZOZY010000407         1116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZOZY010000407 DAZOZY010000000
VERSION     DAZOZY010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.410"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            83..1114
                     /locus_tag="ACHURQ_004424"
     CDS             83..1114
                     /locus_tag="ACHURQ_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR0826008.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          227 t
ORIGIN      
        1 agatcacttt gagggaactt agcccggatt gtgcgatctg atcaatcgcc aaatcaaaac
       61 aaatcaccaa ccggactgag caatgccgat catagcacca atttcccgtg acgaacgacg
      121 cctgatgcag aaagccatcc ataaaacaca cgataaaaat tatgcccgca gactgactgc
      181 catgctgatg ctgcaccggg gcgaccgtgt cagcgacgtt gccagaacgc tctgctgcgc
      241 ccgttcctct gttggacgct ggattaactg gttcacgcag tcgggtgttg agggactgaa
      301 atcattacct gccgggcgtg cccgtcgctg gccgtttgag catatctgca cactgttacg
      361 tgagctggta aaacattctc ccggcgactt tggctaccag cgttcacgct ggagtacaga
      421 actgctggca ataaaaatca atgagataac cggttgccag ttaaatgccg gaaccgttcg
      481 ccgctggttg ccgtctgcgg ggattgtgtg gcgaagggct gcgccaactc tgcgtatccg
      541 tgacccgcat aaagatgaaa agatggcagc aatccataaa gcactggacg aatgcagcgc
      601 agagcatccg gtcttttatg aagatgaagt ggatatccat cttaatccca aaatcggtgc
      661 ggactggcaa ctgcgcggac agcaaaaacg ggtggtcacg ccgggacaga atgaaaaata
      721 ttatctggcc ggagcgctgc acagcgggac aggtaaagtc agctgtgtgg gcggcaacag
      781 caaaagttcg gcgctgttca tcagcctgct gaagcggctt aaagcgacat accgtcgggc
      841 gaaaaccatc acgctgatcg tggacaacta cattatccac aaaagccggg aaacacagag
      901 ctggctgaag gagaacccga agttcagggt catttatcag ccggtttact cgccatgggt
      961 gaatcatgtt gaacggctat ggcaggcact tcacgacaca ataacgcgta atcatcagtg
     1021 cagctcaatg tggcaactgt tgaaaaaagt tcgccatttt atggaaaccg tcagcccatt
     1081 ccccggaggc aaacatgggc tggcaaaagt gtagcg
//
LOCUS       DAZOZY010000408         1116 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZOZY010000408 DAZOZY010000000
VERSION     DAZOZY010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.411"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(156..710)
                     /locus_tag="ACHURQ_004425"
     CDS             complement(156..710)
                     /locus_tag="ACHURQ_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR0826009.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(707..997)
                     /locus_tag="ACHURQ_004426"
     CDS             complement(707..997)
                     /locus_tag="ACHURQ_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR0826010.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(997..>1116)
                     /locus_tag="ACHURQ_004427"
     CDS             complement(997..>1116)
                     /locus_tag="ACHURQ_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0826011.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
BASE COUNT          271 a          318 c          212 g          315 t
ORIGIN      
        1 gcaaactcat ctgaccacca gcacgtttaa cgtcctgtgc cgtttttcag gtataaaaaa
       61 acccgcataa agcgggctct ttcaaatgtc catgtctgct attcgcctcg cggtacagct
      121 ttgcgaagcg tacaagaatt gaagcagttt ttacgtcaaa aagcaataac ttttttctct
      181 ataccaaaag ccataaccat tggtttgtac aaaataaatt ctgccacctt tagccaatgc
      241 tcaatgcgtc tttcacaggt tcttaaactc catttcgggt gtgcatcatt cagcagttca
      301 gccattttgc gtttggtcat cccccgcccc tcataccgtt gccgaaggac gctaatcaat
      361 cctggatgct ctgccagcac ctcacttatg acccgatcaa tacataacgc ctctgcatca
      421 gtacaatgcg ccagccagct cttttgctta ccgttaatca tatcccgcaa aaaagcctca
      481 agttcagact tgttcagacc tgcttttttc atcctccgga gcgcctcgtt aattgccgtt
      541 tttgtcagct ttttagaggt caacatcagg ttaaacatat tccccgtctt accgccacca
      601 atatacgacc agcgccccca catacgtagt tttccctgaa tccagacact ttccagcgtg
      661 gtgagacgaa ggtgttctcc gctttttcct gtattcgttg ggtaaatcat aaatatccct
      721 cctttctcca gatttcttgt gtgcgaaaaa caccttctgc atgcatcagg cgtaattctt
      781 ctttggtgta atcgctggtt tttacccgcc cgtcgattaa atcgtggcac gagctacagg
      841 caatcgctgc ctgcatatcg tgtggcttta tcgctgttcc gcacgttccc gccagtcggt
      901 aatgcgccag cacagacgtt tccggatcgt gattgcagta gccaggaatt ctgacggtgc
      961 acatctgccc ccgcgccgct ttacgtaaat ccaccattac gcaaactcca gcagctgcgc
     1021 ggccacattt tcgacttgtt ccggagagga aaatttacgg aacagaatcc agttccacag
     1081 cacattcaga acagatttat aaacctgctg aaactc
//
LOCUS       DAZOZY010000409         1115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZOZY010000409 DAZOZY010000000
VERSION     DAZOZY010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.412"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>1115)
                     /locus_tag="ACHURQ_004428"
     CDS             complement(<1..>1115)
                     /locus_tag="ACHURQ_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR0826012.1"
                     /translation="QLNQSDNFLDSLREFFAILNSSRKGELSTWDTIYLHLILAINAD
                     SDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDNEVEIKAGNERISIGI
                     RNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHTIGSKIANVIFEKLYS
                     RIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIALMNTFYDSDNTSGEV
                     LNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTVFNLFPEM
                     RSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLVNLKTLLN
                     DGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKK"
BASE COUNT          355 a          211 c          166 g          383 t
ORIGIN      
        1 cattttttca atatgctttc attcacaaca gtgtgtgata agtagagatt acttatgttt
       61 tgctttatta atgccacgtc attaaaatga ctatatttaa tgaaaaaacc atcattgagt
      121 agtgttttaa gattgactaa attacttgca tcttcacgta ccatcgtggc aactggaaca
      181 ggcagagaca tgaaaacttc ttcattcacc atcaagtgaa tcatgcattg cgttcccttt
      241 gtcatatcca cacatacgct acaaatatcg tggtaatgcg aagagcgcat ttcaggaaac
      301 agattaaaca cggtatcttg aaaggcacta aatcgtgtta ataaatcctg ataatgaaca
      361 ggttttgttt caaaattatc atcagcaaat ccaagcacta ttgtatgctc tacatctaac
      421 gcagtttcaa atggctcttc ttctgtaaaa tcattattta aaacctcacc tgacgtatta
      481 tcactgtcat aaaaagtgtt catcaatgcg atattatttg ttacagcgtc tttttcgata
      541 ttctcactaa ttgcatatcg cctgctataa tcataaaaat gaataaacgc gccctttata
      601 aaggatatat attgttcttt gttaaagtca atacggctgt ataatttttc aaaaatcaca
      661 ttcgcaattt tactaccaat ggtatgcctg tataataatg ggtgcgattc aatcgtaaaa
      721 tgcttgtcta attgatcttg taatttattc tcatagtcca gagggaaaag aaactgttgt
      781 ctttcaagtt tcccattcga tacattacgt attccaatag agattctttc attacccgcc
      841 tttatttcaa cttcgttatc ttctgattta cccagatatt ttttaacatc agtctcgaaa
      901 gcattactaa aaaaagtatt aaattggtaa ttcgctgatg gcatattttc cgcgagcaac
      961 acatcatttt ttatgagatc actgtcagca tttattgcta atatcagatg caagtagatg
     1021 gtatcccatg tagacaactc acccttacga ctggaattta atatcgcgaa aaattccctc
     1081 agtgaatcca ggaaattatc actttgattt agttg
//
LOCUS       DAZOZY010000410         1115 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZOZY010000410 DAZOZY010000000
VERSION     DAZOZY010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.413"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHURQ_004429"
     CDS             complement(<1..643)
                     /locus_tag="ACHURQ_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0826013.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKFFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHURQ_004430"
     CDS             complement(766..>1115)
                     /gene="tabA"
                     /locus_tag="ACHURQ_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR0826014.1"
                     /translation="LSISWSHYLFYLISEDMTEPHEARRTEYHARYLDIQIVLKGQEG
                     MTFSTQPAGTPDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAP
                     AQVRKAVVKMLMA"
BASE COUNT          323 a          252 c          196 g          344 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 agaactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaaacagg taatgactcc aacttattga tagtg
//
LOCUS       DAZOZY010000411         1103 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZOZY010000411 DAZOZY010000000
VERSION     DAZOZY010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.414"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            207..626
                     /locus_tag="ACHURQ_004431"
     CDS             207..626
                     /locus_tag="ACHURQ_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR0826015.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            626..>1103
                     /gene="umuC"
                     /locus_tag="ACHURQ_004432"
     CDS             626..>1103
                     /gene="umuC"
                     /locus_tag="ACHURQ_004432"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR0826016.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT          271 a          234 c          288 g          310 t
ORIGIN      
        1 tgcttctatt ctattttttt gccgggtgat atttttcatt tctgctggat gagcgtcgtc
       61 gccagaaggc cacgtgagca caggataaga gaacgaaaaa tcagcagtct atgcagcgac
      121 aaatattgat agcctgaatc agtattgatc tgctggcaag aacagattac tgtatataaa
      181 aacagtataa cttcaggcag attattatgt tgtttatcaa gcctgcggat ctccgcgaaa
      241 ttgtgacttt tccgctattt agcgatcttg ttcagtgtgg ctttccttca ccggcagcag
      301 attacgtcga acagcgcatc gatctgaatc aactgttgat ccagcatccc agcgcgactt
      361 acttcgtcaa agcaagtggt gattctatga ttgatggtgg aattagtgac ggtgatttac
      421 tgattgtcga tagcgctatt accgccagcc atggtgatat tgtcatcgct gctgttgacg
      481 gcgagtttac ggtgaaaaaa ttgcaactac gcccgacggt acagcttatt cccatgaaca
      541 gtgcgtactc gcccattacc atcagtagcg aagatacgct ggatgtcttt ggtgtggtga
      601 tccacgtcgt taaggcgatg cgctgatgtt tgctctctgt gatgtaaacg cgttttatgc
      661 cagctgtgag acggtgtttc gccctgattt atggggtaaa ccggtggttg tgctatcgaa
      721 taatgacggt tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggtg ttaaaatggg
      781 cgatccctgg ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag
      841 caattatgag ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc
      901 gccccgcgtc gagatttaca gtattgatga ggcattctgc gatctgacag gtgtgcgtaa
      961 ttgtcgcgat ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca
     1021 tcttactgtt ggtatgggga tcgcccagac caaaacgctg gctaagcttg ccaatcatgc
     1081 ggcaaaaaaa tggcagcggc aga
//
LOCUS       DAZOZY010000412         1088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZOZY010000412 DAZOZY010000000
VERSION     DAZOZY010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.415"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            529..>1088
                     /gene="fimB"
                     /locus_tag="ACHURQ_004433"
     CDS             529..>1088
                     /gene="fimB"
                     /locus_tag="ACHURQ_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR0826017.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          189 c          233 g          333 t
ORIGIN      
        1 tccaggataa aaaggaatgt aacaatctca tggcgtaagc tgacgaacca ggcaggaata
       61 atcgctaggg acctaagcat tagcatgata atagccacta agaaattact gtgttccatg
      121 aaatagccat tttgtggcaa atggagttga ctaataatgt catatgtgag acggctagtt
      181 gaacgaatat taaattttgc tgaatttttt atgttgattt tacttgttac agaacatatc
      241 acatgatata tagataagat tagttgcatt aatgatgagg gttattatta gattcgtatc
      301 aggttgataa atatataaag gtacatagca tgcaagagca tggcgtttgt atggcaaagt
      361 tattataagt aactatcgct actccattta agttcgctca gaagaactgg tccacttacg
      421 ttagttatta agcaaacgtt cgcttttata aacataatca ggattaaaat gttggattat
      481 tgctaaccca gcacagctag tgcgcgtctg taattataag ggaaaacgat gaagaataag
      541 gctgataaca aaaaaaggaa cttccttacc catagtgaaa tcgagtcact ccttaaagca
      601 gcaaataccg ggcctcatgc agcacgtaat tattgtctga ctttgctttg ttttattcat
      661 ggtttccggg cgagtgaaat ttgtcgattg aggatttcgg atattgatct taaggcaaag
      721 tgtatatata tccatcgatt aaaaaaaggc ttttcaacaa cgcacccgct attgaacaaa
      781 gaagttcagg ctttaaaaaa ctggttgagt atccgtactt cttatccgca tgctgagagc
      841 gagtgggtat ttttatcacg taaggggaat ccgctttctc ggcaacagtt ttaccatatt
      901 atctcgactt ccggtggtaa tgccgggttg tcactggaga ttcatccgca catgttacgc
      961 cattcgtgtg gttttgcttt ggcgaatatg ggaatagata cgcgacttat ccaggattat
     1021 cttgggcatc gcaatattcg tcatactgtc tggtatacca ccagcaatgc agggcgtttt
     1081 tacggcat
//
LOCUS       DAZOZY010000413         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZOZY010000413 DAZOZY010000000
VERSION     DAZOZY010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.416"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHURQ_004434"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHURQ_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHURQ_004435"
     CDS             294..>1087
                     /locus_tag="ACHURQ_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0826018.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZOZY010000414         1086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZOZY010000414 DAZOZY010000000
VERSION     DAZOZY010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.417"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(506..>1086)
                     /locus_tag="ACHURQ_004436"
     CDS             complement(506..>1086)
                     /locus_tag="ACHURQ_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0826019.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          343 a          207 c          190 g          346 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacggt
//
LOCUS       DAZOZY010000415         1055 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZOZY010000415 DAZOZY010000000
VERSION     DAZOZY010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.418"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            71..1015
                     /gene="leuO"
                     /locus_tag="ACHURQ_004437"
     CDS             71..1015
                     /gene="leuO"
                     /locus_tag="ACHURQ_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR0826020.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          285 a          221 c          254 g          295 t
ORIGIN      
        1 atattcttca ggattatttc tctgcattcc aataagggaa agggagttaa gtgtgacagt
       61 ggagttaagt atgccagagg tacaaacaga tcattcagag acggcggagt taagcaagcc
      121 acagctacgc atggtcgatc tcaacttatt aaccgttttc gatgccgtga tgcaggagca
      181 aaacattacc cgtgccgctc atgttctggg tatgtcgcaa cctgcggtca gtaacgctgt
      241 tgcacgcctg aaggtgatgt ttaatgacga gctttttgtt cgttatggcc gtggtattca
      301 accgactgct cgcgcatttc aactttttgg ctcagttcgt caggcattgc aactagtaca
      361 aaatgaattg cctggttcag gttttgaacc cgcgagcagt gaacgtgtat ttcatctttg
      421 tgtttgcagc ccgttagaca gcattctgac ctcgcagatt tataatcaca ttgagcagat
      481 tgcgccaaat atacatgtta tgttcaagtc ttcattaaat cagaacactg aacatcagct
      541 gcgttatcag gaaacggagt ttgtgattag ttatgaagac ttccatcgtc ctgaatttac
      601 cagcgtgcca ttatttaaag atgaaatggt gctggtagcc agcaaaaatc atccaacaat
      661 taagggcccg ttactgaaac atgatgttta taacgaacaa catgcggcgg tttcgctcga
      721 tcgtttcgcg tcatttagtc aaccttggta tgacacggta gataagcaag ccagtatcgc
      781 gtatcagggc atggcaatga tgagcgtact tagcgtggtg tcgcaaacgc atttggtcgc
      841 tattgcgccg cgttggctgg ctgaagagtt cgctgaatcc ttagaattac aggtattacc
      901 gctgccgtta aaacaaaata gcagaacctg ttatctctcc tggcatgaag ctgccgggcg
      961 cgataaaggc catcagtgga tggaagagca attagtctca atttgcaaac gctaactgaa
     1021 tgcagaatag gtcagacatg aatgtctggt ttatt
//
LOCUS       DAZOZY010000416         1042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZOZY010000416 DAZOZY010000000
VERSION     DAZOZY010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.419"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            154..>1042
                     /locus_tag="ACHURQ_004438"
     CDS             154..>1042
                     /locus_tag="ACHURQ_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR0826021.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT          290 a          253 c          269 g          230 t
ORIGIN      
        1 ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca acttcacttg
       61 cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca gcagcgatat
      121 cttatgcaca taatggtgac tcaaggaggg cttatgtcac tgtcaattgt tcatacccgc
      181 gcagccctgg gagtaaatgc gcccccgatc actgttgagg tacatatcag taaaggtcta
      241 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
      301 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
      361 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
      421 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
      481 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
      541 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
      601 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
      661 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
      721 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
      781 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
      841 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
      901 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
      961 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
     1021 atttcgttgg cgcataacgg cg
//
LOCUS       DAZOZY010000417         1033 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZOZY010000417 DAZOZY010000000
VERSION     DAZOZY010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.420"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..1033
                     /locus_tag="ACHURQ_004439"
                     /pseudo
     CDS             1..1033
                     /locus_tag="ACHURQ_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          214 a          339 c          266 g          214 t
ORIGIN      
        1 gtaaagatac gcatcacgct gttgccatta atcgttcagg taaacgcctg ttcgataaag
       61 cattacccaa cgacgaaaac aaactcaggt cgctaatatc tgacctgaaa caacatggtc
      121 agatactgct ggttgttgat cagccagcta ccatcggtgc gttacctgtc gccgttgccc
      181 gctcagaagg agtccttgtc ggatacctcc ctggactggc catgcgccgc atagccgact
      241 tacacgccgg tgaagctaaa actgatgctc gtgacgctgc catcattgcc gaagctgccc
      301 gtaccctgcc tcacgcgcta cgcacgctga aactggctga cgagcaaatc gccgaactct
      361 ccatgctctg cggcttcgat gatgatcttg ccgcacagac aacgcaggcc agcaaccgta
      421 tccgcggcct tctgacccag atacatccgg cactggagcg cgttctcggt ccgagacttg
      481 agcacccggc ggtactcgat cttctccagc gatatccctc accagaaaaa ctcgcttcgc
      541 tgggcttcgc tgggtgagaa gaagctggca gcccagctct gcaaacttgc gcctcgtctg
      601 ggtaaacgcc ttgcagcaga catagctcag gcactggccg aacaaaccgt cgtcgttccc
      661 ggcacgaatg ccgctgccgt agtactgcca cgtctggcac tccagctcat cacgctgcgt
      721 aagcaaagag acgaggtggc gcttgaggta gaacagcgag ttcttgctca ccctctttac
      781 ccggtcctga ccagtatgcc cggagtcggt gtcaggaccg cagccagact cctcaccgag
      841 gtcgcctgcc gcgccttcgc ctctgccgca catctcgctg cttatgctgg ccttgcgccg
      901 gtaactcggc gatccggctc gtcaatacgc ggtgagcatc cctcgcgacg gggtaataaa
      961 gctctcaaac gggcgttgtt cctgtcggcc ttcgccgcgc tcagggatcc gctctccagg
     1021 gcttactaca ccc
//
LOCUS       DAZOZY010000418         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZOZY010000418 DAZOZY010000000
VERSION     DAZOZY010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.421"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHURQ_004440"
     CDS             complement(77..865)
                     /locus_tag="ACHURQ_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR0826022.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHURQ_004441"
     CDS             complement(905..>1023)
                     /locus_tag="ACHURQ_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR0826023.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZOZY010000419          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZOZY010000419 DAZOZY010000000
VERSION     DAZOZY010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.422"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>974
                     /gene="tssI"
                     /locus_tag="ACHURQ_004442"
     CDS             <1..>974
                     /gene="tssI"
                     /locus_tag="ACHURQ_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR0826024.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          250 a          261 c          298 g          165 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZOZY010000420          961 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZOZY010000420 DAZOZY010000000
VERSION     DAZOZY010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.423"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>961
                     /gene="htrE"
                     /locus_tag="ACHURQ_004443"
     CDS             <1..>961
                     /gene="htrE"
                     /locus_tag="ACHURQ_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFR0826025.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQ"
BASE COUNT          278 a          195 c          238 g          250 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 a
//
LOCUS       DAZOZY010000421          946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZOZY010000421 DAZOZY010000000
VERSION     DAZOZY010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.424"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHURQ_004444"
     CDS             complement(<1..304)
                     /locus_tag="ACHURQ_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR0826026.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(744..>946)
                     /locus_tag="ACHURQ_004445"
     CDS             complement(744..>946)
                     /locus_tag="ACHURQ_004445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826027.1"
                     /translation="LHIIGGAGENIILGTMNQVLLPLKQDHPGTRWWCSALEMVLSGG
                     VAIIFYAICLLYSSGEFNKFLI"
BASE COUNT          292 a          218 c          201 g          235 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagct
//
LOCUS       DAZOZY010000422          936 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZOZY010000422 DAZOZY010000000
VERSION     DAZOZY010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.425"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(198..>936)
                     /locus_tag="ACHURQ_004446"
     CDS             complement(198..>936)
                     /locus_tag="ACHURQ_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR0826028.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          280 a          198 c          199 g          259 t
ORIGIN      
        1 attgcttctc cgataaaatg tcagagaagc atgattacgt ggttattgtc tgttgatgac
       61 ttgttgaccg agccgtcatc attctcaatg cattagttaa atcatttgtg atttttaagg
      121 attatcctgt ctatgatttt ctacttagaa ggatatggca ctaactatgc atcttatagt
      181 ttactgctgg catctattta aaatagcaat tgagtcaaat agcccatcaa gatagccata
      241 cctaatataa ccgtcaggaa tgcagctatc atcggcattc tgaacattga tttaagtaat
      301 atcacttccg tcaggcttgc gccggcactg ccgatgatta atgccattaa tgctcccaga
      361 cccattcctt ttgtcatcag aacagatgcc agagggataa ccgcctcagc ccggatatac
      421 agcggaatac cgacaacggc gcttaatggg atggcgaagg gattatctgc tcctgcatga
      481 gcggcaatcc acgctgaggg aataaagcca taaataaaag atcctattaa tactcctaac
      541 aaaagatatg gcagtacatc tttaaactgt tgcaacgcat ctttggtggc tagttttatg
      601 ggattaatac ttactacagc tttggtactg caacaggtaa ctacggggtt ctcaatggct
      661 ttagccgctg gactacaaca actcactttt atgggcgtat atgtcgttcc cggcgaagtt
      721 tttgctggag cacaacaatt tgctgatgag cttttactgg caatgatatg acgttcaaac
      781 cccagagaat ccaggataat actggcaaga acggagacgc cggctgcgat aatcgcgtac
      841 aacaaggtga ctttccaacc aaaggtcatc cacattagtc cgacgataat gggattaagt
      901 aatggtgaaa caaataaaaa agtgagagtc ggacca
//
LOCUS       DAZOZY010000423          906 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZOZY010000423 DAZOZY010000000
VERSION     DAZOZY010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.426"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHURQ_004447"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACHURQ_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFR0826029.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DAZOZY010000424          889 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZOZY010000424 DAZOZY010000000
VERSION     DAZOZY010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.427"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..569)
                     /locus_tag="ACHURQ_004448"
     CDS             complement(<1..569)
                     /locus_tag="ACHURQ_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR0826030.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLA"
     gene            complement(569..>889)
                     /locus_tag="ACHURQ_004449"
     CDS             complement(569..>889)
                     /locus_tag="ACHURQ_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR0826031.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          210 c          194 g          188 t
ORIGIN      
        1 gcaagaccca ttaagatccc tggcaaatat cccgccataa atagcgatgc tacagaaacg
       61 ccgccagctg tcagggaaaa cacaattaac acgtttgagg gcggtatcag taatccagta
      121 atacaagatg agacgtttac agcggtagaa aatgccggat cataaccttg tttggtctga
      181 atgggattca gtgtgccacc aacggcagca gctgccgcaa cggctgaacc agaaatggaa
      241 ccaaacatca tattcgccag gacgttcaca tgaccaagtg aaccaggaac ccgaccaacc
      301 attacctggg cgaggttaat caaacgtatc gcaataccgc cgctgttcat cagcgttccg
      361 gcaaagatga aaaaggggat tgccaatagg gcaaagttat ccagtccatt agccagtcgc
      421 tgcgaaacag tgattgccgc gatatcccag ggaaacatca aaagcattga agctacagtg
      481 gcaataccga ttgagaacga aatgggcaca ccaataaaga ccagcacaaa aaaacagcca
      541 aacagtgtaa tcgcgatata actatccatt aactggcctc ctgcgcaggt tgtttcagtt
      601 gttgaatgct ttgattaata aaacacaagg cataaaacat catcaccaat ccacttaatg
      661 gcagaatgat atatacgtat cccatcggta tttgcatcgc ggcagatacc tgtgatgttg
      721 ccagcgtttt atcaattaat ttcaggccac cagtcacaat gaccgaacca gcaaaaccta
      781 aaataagaac gttaatgact atactaagaa gtaattgctt gcgcttattc agggcgagag
      841 caaataaatc gatagagaga tgtctctgcg cgccgacggt atatgcagc
//
LOCUS       DAZOZY010000425          887 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZOZY010000425 DAZOZY010000000
VERSION     DAZOZY010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.428"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            59..859
                     /locus_tag="ACHURQ_004450"
                     /pseudo
     CDS             59..859
                     /locus_tag="ACHURQ_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          255 a          171 c          214 g          247 t
ORIGIN      
        1 ttggataacg gcacttattg atatattcat gaagattata atcacaaggg aatacataat
       61 gaaaagaaaa gttctggcaa tgctggtccc agcgttatta gttgctggcg cagcaaatgc
      121 ggctgaaatt tataataaaa atggcaataa actggatctg tacggaaaag ttgatgctcg
      181 tcatactttt tccgataacc ctggcgatga cggcgatgaa actatcatcg agttaggctt
      241 caaaggtgag acccaaatta cagatcagtt aactggttat ggtcaagcac tgaccaaaac
      301 aaaggctagc gacactgaag gttctgataa tacttatgtc aaacttgctt ttgcaggtct
      361 gaagttcggc gagatgggct cctttgacta tggtcgtaac tatggtgtga tttacgacgt
      421 tgaagcctgg acagatatgc ttcctgtatt cggcggcgac tcctatacct ggactgataa
      481 cttcatggca ggccgtgcaa atggggtagc tacttaccgc aatagtgatt tcttcggtct
      541 ggtggaaggt ctgaactttg cattgcagta tcaaggtaac aacgaaggca gcaatgcagg
      601 agaagatcaa gaaggtacta aaaatggtca cgaagacgtt cgcttccaga acggtgatgg
      661 ttttggtctt tccacttcat atgactttga cttcggtttg agcttaggtg ccgcttactc
      721 aaactctgac cgtaccaatt ctcaagtagc tcttggcggg tatcactaca atgaatacag
      781 caaatttgct ggtggtgata cagctgaagc atggacattt ggtgcaaaat acgacgccaa
      841 caatgtttat cttgcaatgg gtgatgctgc caacttactg atttagt
//
LOCUS       DAZOZY010000426          874 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZOZY010000426 DAZOZY010000000
VERSION     DAZOZY010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.429"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>874
                     /gene="cadA"
                     /locus_tag="ACHURQ_004451"
     CDS             <1..>874
                     /gene="cadA"
                     /locus_tag="ACHURQ_004451"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR0826032.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKEL"
BASE COUNT          237 a          219 c          212 g          206 t
ORIGIN      
        1 aacttctcac cgatttacga aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa
       61 gtgatttacg aaacccagtc cactcacaaa ctgctggcgg cgttctctca ggcttccatg
      121 atccacgtta aaggtgacgt aaacgaagaa acctttaacg aagcctacat gatgcacacc
      181 accacttctc ctcactacgg tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa
      241 ggcaatgcag gtaaacgttt gatcaacggt tccattgaac gtgcgatcaa attccgtaaa
      301 gagatcaaac gtctgagaac ggaatctgat ggctggttct ttgatgtatg gcagccggat
      361 catatcgata cgactgaatg ctggccgctg cgttctgaca gcacctggca cggcttcaaa
      421 aacatcgata acgagcacat gtatcttgac ccgatcaaag tcaccctgct gactccgggg
      481 atggaaaaag acggcaccat gagcaacttt ggtattccgg ccagcatcgt ggcgaaatac
      541 ctcgacgaac atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc
      601 agcatcggta tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa
      661 cgtgcgttcg acctgaacct gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat
      721 cctgaattct atgaaaacat gcgtattcag gaactggctc agaatatcca caaactgatt
      781 gttcaccaca atctgccgga tctgatgtat cgcgcatttg aagtgctgcc gactatggta
      841 atgactccgt atgctgcgtt ccagaaagag ctgc
//
LOCUS       DAZOZY010000427          872 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZOZY010000427 DAZOZY010000000
VERSION     DAZOZY010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.430"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            73..453
                     /locus_tag="ACHURQ_004452"
     CDS             73..453
                     /locus_tag="ACHURQ_004452"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0826033.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPKTLSISSEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            450..797
                     /gene="tnpB"
                     /locus_tag="ACHURQ_004453"
     CDS             450..797
                     /gene="tnpB"
                     /locus_tag="ACHURQ_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR0826034.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          218 a          235 c          226 g          193 t
ORIGIN      
        1 gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt agtgggaacc
       61 aaagtgtcag atatgcagaa aaatgtgact cccggcaggc gaaaaggctg ccctaattat
      121 cctcccgaat ttaaacagca gctcgttgct gcctcctgtg aacccgggat atccatctca
      181 aaacttgctc ttgaaaatgg cattaacgcc aatctgttgt tcaaatggcg acaacaatgg
      241 cgcgagggaa agctgctatt accttcttca gagagccccc agctacttcc tgtgactctc
      301 gatgcagctg ccgaacagcc agaatcgctc gcagaggacc cgaaaaccct cagtatcagc
      361 agtgaggtaa cgttccggca cgggacgctc cgcttcaatg gcaatgtcag cgaaaagctc
      421 ctgactctgc tgatacagga actgaagcga tgatcccgtt accttccggg atcaaaattt
      481 ggctggttgc cggtatcacc gatatgagaa atggcttcaa cggcctggct gcgaaagtac
      541 agacggcgct gaaagacgat cccatgtccg gccatgtttt cattttccgg ggccgcagcg
      601 gcagtcaggt taaactgctg tggtccacca gtgacggact gtgcctcctg accaaacggc
      661 tggagcgtgg gcgcttcgcc tggccgtcag cccgtgatgg caaagtgttc cttacacagg
      721 cgcagctggc gatgctgctg gaaggtatcg actggcgaca gcctaagcgg ctgctgacct
      781 ccctgaccat gctgtaaatc tctttatcct ggttgtcaca gaataagccc ggtaaaatac
      841 gggcttatga acgacatctc ttctgacgac at
//
LOCUS       DAZOZY010000428          863 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZOZY010000428 DAZOZY010000000
VERSION     DAZOZY010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.431"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..344
                     /locus_tag="ACHURQ_004454"
     CDS             <1..344
                     /locus_tag="ACHURQ_004454"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR0826035.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..777)
                     /locus_tag="ACHURQ_004455"
                     /pseudo
     CDS             complement(406..777)
                     /locus_tag="ACHURQ_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
     gene            complement(795..>863)
                     /locus_tag="ACHURQ_004456"
     CDS             complement(795..>863)
                     /locus_tag="ACHURQ_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR0826036.1"
                     /translation="FSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          234 a          207 c          193 g          229 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaaaacg
      781 gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat gactttgtca
      841 tgcagctcca ccgattttga gaa
//
LOCUS       DAZOZY010000429          860 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZOZY010000429 DAZOZY010000000
VERSION     DAZOZY010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.432"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..284)
                     /locus_tag="ACHURQ_004457"
     CDS             complement(<1..284)
                     /locus_tag="ACHURQ_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFR0826037.1"
                     /translation="MNNCIFFSISASTVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYT"
     gene            complement(301..>860)
                     /locus_tag="ACHURQ_004458"
     CDS             complement(301..>860)
                     /locus_tag="ACHURQ_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0826038.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          249 a          182 c          151 g          278 t
ORIGIN      
        1 catgtatagg attcggaaag tccagcatct attttgtacc tcattatatt agataaggat
       61 gtgagaaatt ccttgttatc attcgcagcc atatatgcca gtccgagttc agcctctgcg
      121 gccttacagg caaaatagct tagtgaacac gcttttcgtc cctctttagt actggctgat
      181 attagtgccc ataatattgc tttgtctgcc aaatctaact ctctctgaaa ttgtcttgca
      241 gtaactgtac tcgcagaaat agagaaaaaa atacaattat taataacttt gccatatttt
      301 ttagtccgta actgttaatg tgctatctgg ctctgaaaat attaactcat ttaatttttg
      361 catgtcatta gatgaaccag ttatacaacc ctcggaacag aatcgaggtc ctaaagacgg
      421 accgaaagcg tttaaacaac tttgtgttaa aaatccatca cgattatatg tttctgtatt
      481 tgctgacggt actaaacgta taccattagg ttttctattg gttgctccag gaccattaac
      541 attccagctc catacccctt gaggtattgg acctctattt tgtaagtgtg tacaaccagg
      601 attattttta cattgcatat tattaccatt attaccagag gcgacaggaa tagtaagcac
      661 atcataagag ttatcatcat tttgcgtatc tgaaacgcat gaaagcatac ttaaagttat
      721 gctgtataaa caggtcgcta atcccaacgg gtcaatatca gttatcggat tcaacggata
      781 ctgataaaaa ttccatcccc ccttcagtcc aatcttttcg cgccggcgtg tacaccgggt
      841 ccatctggtt ttgcatctgc
//
LOCUS       DAZOZY010000430          853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZOZY010000430 DAZOZY010000000
VERSION     DAZOZY010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.433"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..286
                     /locus_tag="ACHURQ_004459"
     CDS             <1..286
                     /locus_tag="ACHURQ_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826039.1"
                     /translation="CSTELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKY
                     ENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            298..747
                     /locus_tag="ACHURQ_004460"
     CDS             298..747
                     /locus_tag="ACHURQ_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR0826040.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          285 a          100 c          218 g          250 t
ORIGIN      
        1 ttgttcaact gaattaaact cgaaatcaca atcgcctgca ttaggtttgg gacatgagtt
       61 tgctcatgcg cagtattgcc ttcttgataa ggaaaatttc atggctcttt taagccgaac
      121 agataagaaa tatgagaata aagaagaggc aagggttatt acaataattg aatcgcgtgc
      181 ggcgaaaact ttaggtgagt gtaccagagg agctcatagt ggactgcctt tttatcgggt
      241 ggatggaccg ttgcagacga tgaaaataac gggtacgcca gaatagtgag gttttttatg
      301 atgaaatatt tgatggtttt attgtctcta ttttctggtt cggtattggg gatggggcga
      361 gtgaatgaat tgtgtggaat agatagcgtg aaaacgatag agataattaa tcttccatcg
      421 tatgttacca cgttagttcc gcttagcaag gaaggtctta atgaaatata cagatataaa
      481 gtggtggtga atgaaattag agatctttat gctggtaaga taattgattt attgcaaatg
      541 aagtatttca gaaaagaaaa atataacaat ataagatggg ggggtagtat catttcgaaa
      601 ggtaataata aatgtgaaat ttattttgat gcttttggtg aatgtggaag tgtgaatggc
      661 attaacgtgt gttttgaaaa aaatgaaatg ataggttgga ttaaaaaaga aatccctttg
      721 ctgtcgcaaa aaataggtgg gctttaaatg gtagggcaat attcaaagac agtataggta
      781 actatttgta tggccatgta aggtaaaaga aaaggaataa accatgagcg gaaaaccggc
      841 ggcgcggcag ggc
//
LOCUS       DAZOZY010000431          848 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZOZY010000431 DAZOZY010000000
VERSION     DAZOZY010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.434"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..229)
                     /locus_tag="ACHURQ_004461"
     CDS             complement(<1..229)
                     /locus_tag="ACHURQ_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR0826041.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            410..562
                     /locus_tag="ACHURQ_004462"
                     /pseudo
     CDS             410..562
                     /locus_tag="ACHURQ_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(587..>848)
                     /locus_tag="ACHURQ_004463"
     CDS             complement(587..>848)
                     /locus_tag="ACHURQ_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826042.1"
                     /translation="ALSFILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSI
                     ISAAIEGVRCESLSVQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          276 a          153 c          161 g          258 t
ORIGIN      
        1 aacttgaggc actaaaagca acattaatag aacctgggcc agggaaatgg tatttaataa
       61 gtctgtaagg atcaactttt gctgagatat cgcttaggcc gagtgagaga aagtcttgtt
      121 ctaccatatc accatattgc atatcatcag cagaaaaatc atcgaataaa ctaatagttt
      181 cgaaaagtgt acagggaata ttaattggcg gaagtaatat gttattcatt tgcatttgcc
      241 ttggttatat ttaagctcaa gatcaattcg agaacaaatg ctagggtaaa aaaatattta
      301 aaaaagaagt acacttcaca ttttttcatt acatctctga agagaacctc gttaatgatt
      361 ggtcttgctc attgttaata acaaaatgtt gaatttaccg attaggaaac tttttcctac
      421 gcggattgcc tccagctatg gcatgaactt tgagtttatg ccagaactga agtggagctt
      481 tggctaccct ggcgcgatta tctttatgat ccttgcgggc ctgacaccgt atctgtactt
      541 taagcgtaag aactggctgt aactatacga tgtcgctgtg agtaagttag tgtgctgaca
      601 gcgacatttt agccgaggga atcgcagata acataattgt tgtgaataat atccatgcca
      661 cgttgagttg gacgcttaat gactcacatc tcaccccttc aattgctgca gagataatag
      721 aaaaaagtgt caaacccaat gaaatcaata caaagtagat tgccttacat ccagtatctc
      781 ttattctttt tgctccaata ttgatattta cataggtatt attaataacc agaataaaac
      841 tcaaagcc
//
LOCUS       DAZOZY010000432          842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZOZY010000432 DAZOZY010000000
VERSION     DAZOZY010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.435"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHURQ_004464"
     tRNA            54..130
                     /locus_tag="ACHURQ_004464"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHURQ_004465"
     tRNA            173..248
                     /locus_tag="ACHURQ_004465"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
     gene            432..>842
                     /locus_tag="ACHURQ_004466"
     rRNA            432..>842
                     /locus_tag="ACHURQ_004466"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          238 a          177 c          232 g          195 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc
      541 gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg
      601 ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc
      661 gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg
      721 tgttagtgga agcgtctgga aaggcgcgcg atacagggtg acagccccgt acacaaaaat
      781 gcacatattg tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg
      841 gg
//
LOCUS       DAZOZY010000433          834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZOZY010000433 DAZOZY010000000
VERSION     DAZOZY010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.436"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(354..461)
                     /locus_tag="ACHURQ_004467"
     CDS             complement(354..461)
                     /locus_tag="ACHURQ_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR0826043.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          169 a          252 c          195 g          218 t
ORIGIN      
        1 caacgtttcg cggctattct tgagttgttc aggttcaaga atggcccccg tgatgttgtc
       61 aggtgcatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat
      121 aaagcccccg caacattgcg cctcaccgga acccttccgg cttgacgctg attctacgac
      181 tgtttttctg ttccggaatc cgtcacgtct tcactctgtg ctgcgcattc cgcagaatat
      241 tatccgcttg caaccgttgc atttttctta cgaaaaaggc cgcagagttt cacccctgcg
      301 gcctgatccg ggcgcaaatt gccattacgg cagcctgacg cccgcatggc acgttacttc
      361 cgcttgttca gccagttcac gatcatactg gcaagaatac cagcaatgac cggagccgct
      421 aaatcatgcc agaaggccat gcccagttct gcgagcgtca tatagccgcc tgtattgtaa
      481 tgacaacgtt tcgcggctat tcttgtattg tttggatgca agaatggccc ccgtggtgtt
      541 gtcaggtgca tacctgcaac gtgcgggggt tttctctctc cagcaaccaa tgccaccagg
      601 gattaagccc ccgcaacatt gcgcctcacc ggaatccttc cggcttgacg ctgattctac
      661 gactgttttt ctgttccgga atccgtctca tcttcactct gtgctgcgca ttccgcagaa
      721 tattatccgc ttgcaaccgt tgcatttttc ttacgaaaaa ggccgcagag tttcactcct
      781 gcggcctggt ccgggcgcaa attgccatta cggcagcctg acgcccacat gaca
//
LOCUS       DAZOZY010000434          799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZOZY010000434 DAZOZY010000000
VERSION     DAZOZY010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.437"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHURQ_004468"
     CDS             complement(<1..741)
                     /locus_tag="ACHURQ_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR0826044.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZOZY010000435          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZOZY010000435 DAZOZY010000000
VERSION     DAZOZY010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.438"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHURQ_004469"
     CDS             complement(<1..458)
                     /locus_tag="ACHURQ_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR0826045.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAAQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHURQ_004470"
     CDS             complement(530..742)
                     /locus_tag="ACHURQ_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826046.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          174 g          219 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgcgctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAZOZY010000436          784 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZOZY010000436 DAZOZY010000000
VERSION     DAZOZY010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.439"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>784
                     /locus_tag="ACHURQ_004471"
     CDS             <1..>784
                     /locus_tag="ACHURQ_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR0826047.1"
                     /translation="VSELTRKVSVGTSRKKVILGIILSSIYGSYGETAFAAMLDINNI
                     WTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVPDFSAVSNKGATTSIGGAY
                     SVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRLNKFVVETTGVTESADFSL
                     SPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLFGQNYNPDLLSASLFNLDW
                     KNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHGVTS"
BASE COUNT          261 a          151 c          160 g          212 t
ORIGIN      
        1 tgtttctgaa ctgacccgga aagttagtgt cggtacatca agaaagaaag ttatcctcgg
       61 tattatttta tcctcaatat atggaagtta tggcgaaaca gcatttgcag caatgctgga
      121 tataaataat atatggaccc gcgattatct tgaccttgct caaaacagag gagagttcag
      181 accgggtgca acaaatgttc aattaatgat gaaagatgga aagatatttc attttccaga
      241 actacctgta cctgattttt ctgctgtttc caacaaaggt gcaacaacat caattggagg
      301 tgcgtacagt gttactgcga ctcataacgg tacacagcat catgcaataa caacacagtc
      361 atgggatcag acagcatata aagcaagtaa cagagtatca tctggcgact tttcggttca
      421 tcgtctgaat aaattcgtcg tggaaacaac aggggttacg gagagtgccg acttctcact
      481 ttctcccgaa gatgcgatga aaagatatgg cgtaaactac aacggtaagg aacaaataat
      541 tggcttcaga gcaggtgccg gaacaacctc aacgatatta aacggcaaac aatatctgtt
      601 tggacaaaac tataatcccg acttgttaag cgcaagtctt tttaatctgg actggaaaaa
      661 caagagttac atttatacca acagaacccc ttttaaaaac tcaccaattt ttggcgatag
      721 tggttctggt tcttatctat atgataaaga acaacaaaaa tgggttttcc atggtgttac
      781 cagt
//
LOCUS       DAZOZY010000437          783 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZOZY010000437 DAZOZY010000000
VERSION     DAZOZY010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.440"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..623
                     /gene="yahL"
                     /locus_tag="ACHURQ_004472"
     CDS             <1..623
                     /gene="yahL"
                     /locus_tag="ACHURQ_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR0826048.1"
                     /translation="KLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYPDHPVK
                     FVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDCKCILLEH
                     HMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVFPESLV
                     MLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          228 a          157 c          167 g          231 t
ORIGIN      
        1 acaaactcat tcatctcatg gctgccgtac gagactgtga gtggtcagac gataacgcac
       61 tcaccataaa tgtgcagttt aatgatttcc caggatttta tgactggatg gattaccctg
      121 atcacccggt taagtttgtt tttcgcatac ttgaaaatca aaaagggaca gtttgggtat
      181 atgaccagga tgatgcgttt cttgatatta aagcaaatgt gcaggctgga cgctttaccg
      241 ggcttaagaa attagtgcag tttatcaatt cagtgcgaac agattgcaaa tgtattcttc
      301 ttgaacacca tatgcctttg ctgagacttt tccctaaagg gaaagagtgt atgcatgtgg
      361 aaaaatggct tcgcgaaatg tcgtctattc cggagacaga cgcgcccatt aagcaggcgc
      421 ttgcgcatgg attattatta cacttaaaaa atatctaccc tgtttttcct gaatcactgg
      481 tgatgttatt gctctctgtt ctggacgtaa aaacatacag agatgatgca cgattaaatg
      541 aatggatatc taatagagtg caggaattag gtgacagata ttatcctgta aataagcacg
      601 ttaaaatccg ctacactctt tagttcagaa agggttccac ctgcaagttg ttattccacc
      661 agagagtgaa tcgcaaagca ggtaatcatt tcatctgtat atccgtatgc caaatggcat
      721 tccaccgcct tttcgccgat attttgttgt gatcggcgac acttcgctta aaaaagcacc
      781 agc
//
LOCUS       DAZOZY010000438          780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZOZY010000438 DAZOZY010000000
VERSION     DAZOZY010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.441"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>780)
                     /locus_tag="ACHURQ_004473"
     CDS             complement(<1..>780)
                     /locus_tag="ACHURQ_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0826049.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIE"
BASE COUNT          146 a          259 c          175 g          200 t
ORIGIN      
        1 tactctatat ttatagtttc ttcagagctt gataaaaaat ctatagtttc tttcatttgt
       61 gaatcttgtt ttaaatactc aacagcctga ttaaaatcac ttggatcacc gttaacatgg
      121 aattttaaac ccaaagaatc tataaaccga acagggttta atggatattt atacagattc
      181 cattcacctc tcagccctat cggatcctgg gtgatatacc ttccctgcaa cgggtcatag
      241 taccggttgc ggttgtagta cagccccgac tcctcatcat actgctgccc cggcagacgg
      301 agtgactgct ggaggtgctg cgcgctggtt tcgcccagca ggtttcccca ttcgtcatac
      361 tccccctgcc acgccgtttc cccttccggg ctgatgagcg ccagcggcag tccccggtgg
      421 tcgcagtggt aaagatgaag tttcctctcc gggatgtatt ccgcctccag ctgcgcggcc
      481 atctgctccg ccgtcagccc gcactgcgca agccatgcct cactctccgc actcaccgct
      541 cccgcgcgca gttcccgctc cagcctcccc agcatcaccg acagctccgc cggtagcgtc
      601 acacccgtgt cctcctgcaa cacctccgcc aggctccggt gccgcgcctt cgcctgttcg
      661 ccattctctg tttcgattct caggagcggc gtgaagctcc ccggctggta taccgtctgg
      721 atacgcgtgg tgccggtctg tatcgtcgtc agcctgtcac cgtcccaccc gtaccatgtc
//
LOCUS       DAZOZY010000439          780 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZOZY010000439 DAZOZY010000000
VERSION     DAZOZY010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.442"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            25..>780
                     /gene="gspA"
                     /locus_tag="ACHURQ_004474"
     CDS             25..>780
                     /gene="gspA"
                     /locus_tag="ACHURQ_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR0826050.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          209 a          181 c          196 g          194 t
ORIGIN      
        1 tcaataatta aaacaggaag ttctatgtct acgagaagag aagttattct ttcctggttg
       61 cgtgagaaac gtcagacctg gcgtctgtgc tatttgttgg gtgaggctgg aagtggaaaa
      121 acctggctgg cgcagcaact gcaaaaagat aaacatcgcc gtgtgattac tttaagcctc
      181 gttgtttcct ggcaaggtaa ggccgcatgg atcgttaccg acgataacgc ggctgaacag
      241 ggctgccgtg acagcgcctg gacgcgagat gagatggcgg ggcaattact gcatgcgctt
      301 catcgaactg atagtcgctg ccctcttatt attattgaaa atgctcacct gaatcaccgc
      361 cgcatacttg atgatctcca acgcgcgata tctcttattc ctgacggtca atttttgctg
      421 atcggcagac ccgatcgaaa agtcgaacgt gattttaaaa aacagggcat tgaacttgtc
      481 tcgacaggac gtctgacgga gcacgaactt aaggcaagca tccttgaagg tcagaatatt
      541 gaccaacccg atctcctttt aacctccaga gttctgaaac ggatagcttt attatgtcgg
      601 ggcgatcgca gaaagctggc gcttgccggc gaaacaattc gcttattaca gcaagccgag
      661 cagaccagcg ttttcactgc aaaacaatgg cgaatgattt accgcatatt gggtgataac
      721 cgcccccgga aaatgcagct tgccgtggta atgagtggaa ccattatcgc tattacctgt
//
LOCUS       DAZOZY010000440          776 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZOZY010000440 DAZOZY010000000
VERSION     DAZOZY010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.443"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            120..704
                     /locus_tag="ACHURQ_004475"
     CDS             120..704
                     /locus_tag="ACHURQ_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR0826051.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          228 a          152 c          155 g          241 t
ORIGIN      
        1 attttggtaa caaatatcat tgctcattaa tatctagcat ctgcttataa catttgtgtc
       61 atgaatagtc attcttcaaa ccagtagtct gactaatgga attttattca aggattttta
      121 tgtctaaaaa attaggtttt gccctgtctg gtcttatgct ggcaatggtt gcaggtactg
      181 cttctgctga tatggatggt ggtcagttaa atatcagtgg tctggttgtt gataacacct
      241 gtgaaacccg cgttgacggc ggtaataaag atggtctgat cctgctgcaa actgcaactg
      301 ttggtgaaat cgatgctggc gtgctgaatg acaccgttgg cgctaaagcc aaacctttca
      361 gcatcaccgt tgactgcagc aaagcaaatc cgaatccagg tagtacagct aaaatgacct
      421 tcggttctgt tttctttggt aatagcaaag gaacgctgaa caacgacatg tctattaaca
      481 acccgtctga cggcgtgaat atcgctctgc acaatattga tggttccact atcaaacagg
      541 ttcaaattaa caaccctggc gatgtgtata ctaaagccct ggatgcaacg acaaaatctg
      601 ctgtttatga ttttaaagcg tcttacgttc gtgccgtcgc agaccaaaca gcaactgctg
      661 gttatgtaaa aactaacact gcatacacca ttacttatca gtaagtattc accaattaaa
      721 ttgattgaat gaatatacag ggaatagtgt taataatttc tatttttata ttattc
//
LOCUS       DAZOZY010000441          775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZOZY010000441 DAZOZY010000000
VERSION     DAZOZY010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.444"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            91..>775
                     /locus_tag="ACHURQ_004476"
     CDS             91..>775
                     /locus_tag="ACHURQ_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0826052.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQ"
BASE COUNT          248 a          165 c          130 g          232 t
ORIGIN      
        1 aaacaataaa tttcaatcct gaaacatttt atttcagctt attatttccc agtatcacat
       61 tttatcagaa tatccttctg cgaggttatt atgcttcctg taaataatcc ccccctatcc
      121 actggaaacg tctcttttta cagaactaca tcaatcgaca atgttcacaa taattatctc
      181 tccgaatggg ttgaatggac taaaaacagc atttccggag aaaacaggga aactgctttt
      241 acccggctcc aattatgtct ggagaacagt gaaacatcgt tggacttatc ttgtttaggt
      301 ctcagatctc taccacgatt gcctgacaat cttgatgaaa ttaatgtaag caataaccaa
      361 ctatcaatgc tccccgagct accaagggca ttgaaagagc tgaatgcaag cagtaatcaa
      421 ttatctgcac ttcctgaatt accagtgtcg ctggaatata taaatgtgag tgataaccat
      481 ttgttcgcac ttcctgaatt acctgcgtca ctagaatata ttaatgtaag tgacaatcac
      541 ctgtctgtac ttccgaggtt accaatgtca ttggaattac ttgatgcagc cagaaatgct
      601 ttggaagtaa taccagattt tccagaaaga gatgatcata ttataagaat attctggctt
      661 aatcagaacc ggatcacggc aattccggaa agcatacttg gcctcagttc tgatagcgtt
      721 gtcaatctta gagaaaatca actatctccc agaataatgc aaactttgtt acaac
//
LOCUS       DAZOZY010000442          765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZOZY010000442 DAZOZY010000000
VERSION     DAZOZY010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.445"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(81..>765)
                     /locus_tag="ACHURQ_004477"
     CDS             complement(81..>765)
                     /locus_tag="ACHURQ_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR0826053.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQLR"
BASE COUNT          176 a          193 c          189 g          207 t
ORIGIN      
        1 ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact
       61 aaatcagtaa gttggcagca tcaccgtaat tgagttgttg accaatttgt ctgtggcagg
      121 taatcacttg ctgtctttgt ctccatccct ggatactggg gatcatcctg cggcatacaa
      181 gtcagattgg gttttaccca cttcctgcaa tacagtgtgt aatcgtaagg gcttaatgct
      241 ctgaagaatg ttttgaaaac attaggcgca ctactggcct ggtcaatagg atatggcagt
      301 cttaattgtt tcgctatgtc tggcaactta cttggatcgg gcgtaggcgg tttgctgggt
      361 ttcgctccat atccgccatt atttaagtgt ccatatttag atgccagccc cattgaatct
      421 atatactgaa ccggattcaa cggatactga taaaaattcc atcccccctt cagtccaatc
      481 ggatcctgag tgatataccg cccctgcagc gggtcataat agcggtggcg gttgtaatac
      541 aggccggact cctcatcata ctgctgcccc ggcaggcgaa tgagctgctg cagatgatgc
      601 gggttctcat cactcagcag gttgccccat tcatcatatt ctgcgcacca cgctgttgtc
      661 ccttccgtgc tgataagggc cagcggcagg ccgcgatggt cgcagtggta caggtgaatt
      721 tttcgcgccg gcgtgtatac cgggtccatc tggctttgca tctgc
//
LOCUS       DAZOZY010000443          751 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZOZY010000443 DAZOZY010000000
VERSION     DAZOZY010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.446"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            584..>751
                     /locus_tag="ACHURQ_004478"
     CDS             584..>751
                     /locus_tag="ACHURQ_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFR0826054.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DAZOZY010000444          748 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZOZY010000444 DAZOZY010000000
VERSION     DAZOZY010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..748
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.447"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..407
                     /locus_tag="ACHURQ_004479"
     CDS             <1..407
                     /locus_tag="ACHURQ_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0826055.1"
                     /translation="WRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYT
                     QRLKEAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWY
                     NNRRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            449..649
                     /locus_tag="ACHURQ_004480"
                     /pseudo
     CDS             449..649
                     /locus_tag="ACHURQ_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          216 a          175 c          198 g          159 t
ORIGIN      
        1 ggtggcgggt ctcatcgtcc atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tatgggcccg tcgaccgtcc ggcacggtcc atcacagtga taaaggttct cagtatgtat
      121 cgctggccta cacacagcgg cttaaggaag ccggattact ggcatcaaca ggaagtacag
      181 gcgactcgta tgacaacgcg atggcggaga gcatcaatgg tctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccatactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tactcctccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaacg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcaa ccttcacgat aaaaaacttt tcattaaagg
      481 taaagcggat caatatccgg cacttcattc tctgatatct ccgacccgca ttaatctgaa
      541 agaaatagaa attcactggc gtgaagtgct acgtctggcg acctcaataa aacagggaac
      601 agtgacagca tccctgatgc tgagaaaact tgccagttac cccaaacaaa agtaaggcag
      661 tatgactttc cgtgagacag atgctggcca acgtgggata tttaacgcgg gttaacatcg
      721 ttgtcagagc cgcatgggtg agctgttg
//
LOCUS       DAZOZY010000445          746 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZOZY010000445 DAZOZY010000000
VERSION     DAZOZY010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.448"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..400
                     /locus_tag="ACHURQ_004481"
     CDS             <1..400
                     /locus_tag="ACHURQ_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0826056.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT          261 a          138 c          141 g          206 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgt
//
LOCUS       DAZOZY010000446          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZOZY010000446 DAZOZY010000000
VERSION     DAZOZY010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.449"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHURQ_004482"
     CDS             complement(<1..396)
                     /locus_tag="ACHURQ_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR0826057.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          173 c          108 g          229 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcatccatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAZOZY010000447          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZOZY010000447 DAZOZY010000000
VERSION     DAZOZY010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.450"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /locus_tag="ACHURQ_004483"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="ACHURQ_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAZOZY010000448          735 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZOZY010000448 DAZOZY010000000
VERSION     DAZOZY010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.451"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            173..735
                     /locus_tag="ACHURQ_004484"
                     /pseudo
     CDS             173..735
                     /locus_tag="ACHURQ_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          250 a          142 c          129 g          214 t
ORIGIN      
        1 cagagaaata aaaaacaatc tgaagcatgc tctgaaacat cctactctgt cactctaatg
       61 atggcacctt gctcttctta taaagaaggg aacaattaag aatattaaat tttcaaactt
      121 tcatcaactt cacatcaaga aattaaaaaa tttaaacatt aatctaaata ataacaacct
      181 gatcacataa tcgggtacga ttttatccga taactataat gctataactc tgggaaatgg
      241 ttggaataca ccgctgggtg ccatctcttt ttacgctgcc cgatcgagta gtaagttaaa
      301 taatgatacc agacacgaag gtacgagcta tcaggtcgcc tataataaat atctagtaca
      361 aacagccact cgatttagcg tggccgcctg gcgttatgct tcgcaggatt acaggacatt
      421 cagcgaccat ctttacgaaa atgataaaca ctatcatcag agtgattatg atgattttta
      481 tgatattggc agaaaaaata gcctttctgc caatattatg caacctttat ccaataatct
      541 gggaaatgta tcattaagtg cgctttggcg gaattactgg gggcgaagtg gaaatgctaa
      601 agattaccaa ttcagttact ccaataactg gcaacacatt agttatactt tctctgccag
      661 ccaatcttat gatgaaaata ataaagaaga ggagcgtttt aatctgttta tctccattcc
      721 tttctactgg gggga
//
LOCUS       DAZOZY010000449          733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZOZY010000449 DAZOZY010000000
VERSION     DAZOZY010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.452"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            10..733
                     /gene="espL4"
                     /locus_tag="ACHURQ_004485"
                     /pseudo
     CDS             10..733
                     /gene="espL4"
                     /locus_tag="ACHURQ_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT          263 a          139 c          130 g          201 t
ORIGIN      
        1 aattaataaa acgaaaacac gacactcact caaaccattg ctccaatatt tgaagaatac
       61 ctaatggaaa ttttacctca acgctcagac actcttgata aacaagaatt aaacctaaaa
      121 tcagatagaa aagaaaaaga attcccaaga attaaactta atggtcaatg ttattttccg
      181 gggcgacccc aaaaccgtat agtatgccga cacattgctg cacaatatat taatgatatt
      241 tatcagaatg ttgattacaa accccatcaa gatgattatt cttcagctga aaaatttctc
      301 actcacttca acaaaaaatg caaaaaccag actttggcgt tgatttccag ccgtcctgag
      361 gggcgttgcg ttgctgcctg cggtgatttc gggctagtta tgaaagcata ttttgacaag
      421 atggaatcaa atggcatcag tgttatggca gccatattac tggtggataa ccatgctttg
      481 acggtccggc taagaataaa gaacacaact gaaggatgta cccattacgt ggtttcggtt
      541 tatgatccta atgtaactaa cgacaaaata agaatcatga gcgaaagcaa agaggatatt
      601 aaacactatt ctctgatgga ttttatgaat gtagattata gcctcctgaa atggtcaaat
      661 gatcatgtta ttaaccaatc tgttgcaata attccagcac ttccgaaaga acagctattg
      721 atattaaaag gat
//
LOCUS       DAZOZY010000450          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZOZY010000450 DAZOZY010000000
VERSION     DAZOZY010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.453"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHURQ_004486"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHURQ_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR0826058.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZOZY010000451          718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZOZY010000451 DAZOZY010000000
VERSION     DAZOZY010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.454"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            9..718
                     /locus_tag="ACHURQ_004487"
                     /pseudo
     CDS             9..718
                     /locus_tag="ACHURQ_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          212 a          149 c          207 g          150 t
ORIGIN      
        1 acacgtaggg tgaagccggg ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg
       61 tcacgcagag tcagtttgac gccgtgggca ggctcaggac acagacggat gccgcaggcc
      121 ggacaacaga atacagcccg gatgtggtga cgggtctcat cacgcgcatc accacgccgg
      181 atggcagggc atcggcgttt tactataacc accacagcca gttaacgtca gccaccgggc
      241 ctgacgggct ggaaatacgc cgggaatatg atgaatgggg caacctgctg aatgaagaga
      301 acccgcatca gctgcagcag cttattcgtt tgccggggca gcagtatgat gaggagtccg
      361 gcctgtatta taaccgccac cgctattatg acccgctgca ggggcggtat atcactcagg
      421 atccgattgg gctgaagggg ggatggaacc tgtatacata tccgctgagc ccggtgaata
      481 gcatggatcc attaggatta tatgaattta aatcaaaaaa tattgatgat attggaatat
      541 ttgcattggc aatgtgtaat ggagaatcaa ttaacgagaa taaagaatat ggtggactaa
      601 tatgtaagaa gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata
      661 gtgtagactt gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatca
//
LOCUS       DAZOZY010000452          708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZOZY010000452 DAZOZY010000000
VERSION     DAZOZY010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.455"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            283..687
                     /gene="stpA"
                     /locus_tag="ACHURQ_004488"
     CDS             283..687
                     /gene="stpA"
                     /locus_tag="ACHURQ_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR0826059.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          213 a          140 c          158 g          197 t
ORIGIN      
        1 ttcttttatt ctgttatatt gcatttttat tcacttcggt tttgtcaggc ttgcggaatt
       61 agcgagcaga gagcgccgtt ctgttcacca ataatctaaa ttactcatca aaatggctaa
      121 tatataatct tgaaattatt ctgagagttt cagaaaataa cagctgaaat aatctcgcgc
      181 aagactgtaa atagattaaa ttttgtggaa atataataag tgatcgctta cactacgcga
      241 cgaaatactt ttttgttttg gcgttaaaag gttttcttta ttatgtccgt aatgttacaa
      301 agtttaaata acattcgcac cctccgtgcg atggctcgcg aattctccat tgacgttctt
      361 gaagaaatgc tcgaaaaatt cagggttgtc actaaagaaa gacgtgaaga agaagaacag
      421 cagcagcgtg aactggctga gcgtcaggaa aaaattagca cctggctgga gctgatgaag
      481 gctgacggaa ttaacccgga agagttattg ggtaatagct ctgctgctgc accacgcgct
      541 ggtaaaaaac gccagccgcg tccggcgaaa tataaattca ccgatgttaa cggtgaaact
      601 aaaacctgga ccggtcaggg ccgtacaccg aagccaattg ctcaggcgct ggcagaaggt
      661 aaatctctcg acgatttcct gatctaatta ctgaggccgg atgtcgct
//
LOCUS       DAZOZY010000453          704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZOZY010000453 DAZOZY010000000
VERSION     DAZOZY010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.456"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
BASE COUNT          217 a          172 c          135 g          180 t
ORIGIN      
        1 gttgatattt tttccatctg atacatatcc cgccccccga cagacctgtg ctatcggaaa
       61 gaaaaaaggc catcagcaaa actctgatgg cctgaatcac ctttaccaat attgtatgaa
      121 aaaacacgca cgactaattg acaataattt tcatttccat taaaaaagat cgcgtagcat
      181 tcttaattca tgaggaactt tacccgccag caatctgagt agcgaaagct gttcatcccc
      241 aatgtttttg ctggcgggtc cttttttcta cggtcctcct ccccggaggg gggcatttca
      301 ttatttttct aatcgttact ggtgagaagg cggaagaaaa agccagttct tcgcccctaa
      361 ataacagcat gattccagtc catgcaaaat ctgatttcca gctcacacat accgagcgct
      421 gtacaaaaaa ccgccagcca aggcagttaa gagtgtgttc ccggggtttg cttagaatat
      481 tttgtaagtt gtccgaagag atatttacaa caccagaatg atgaatcatc agtcccctgc
      541 cagaggttca ctacgtaacc tgaccgacaa tgcaccattc tggtgctgaa aaaagagcac
      601 tggaactgca acaaaacaac atcacagaac aggagatggt aaggagtcag acattcccac
      661 acaatattgt gtcagtgcaa ataacaacct tcgtctcaaa tctc
//
LOCUS       DAZOZY010000454          704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZOZY010000454 DAZOZY010000000
VERSION     DAZOZY010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.457"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>704
                     /locus_tag="ACHURQ_004489"
     CDS             <1..>704
                     /locus_tag="ACHURQ_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR0826060.1"
                     /translation="LIDPKYFADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADV
                     VCKGSLKPLTEIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKW
                     HEANLTPYLLTLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDR
                     SHRKYQQTITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVA
                     EMQKQYKHLFAHGEYI"
BASE COUNT          216 a          138 c          140 g          210 t
ORIGIN      
        1 tttaattgac ccaaaatact ttgctgatct gccgacgtca cagttttggt cgtacgcgac
       61 atacttcagg gtattgtcct ttgaatatct gagtgaaagt atttccacac tgctgtatct
      121 ggatgccgat gtcgtttgta aaggaagtct gaagcctctc acagaaatta tatttaaaga
      181 tgagtttgct gcggtcattc ctgacaatgt tagtactcag gcggcatgtg caaaacgcct
      241 caacattccc gagatgaatg gacgttattt caatgcaggc gttatctatg tcaatcttaa
      301 aaaatggcat gaagcaaatt tgacaccgta tttactcacg cttttacgag gggaaactaa
      361 atatggctct cttaaatatt tagatcagga tgcgttgaat atcgcattta atatgaataa
      421 tatctacctc gcgaaggatt ttgatactat ttataccctg aaaaacgaac ttcatgatcg
      481 tagtcatcga aagtatcagc aaaccattac cgataaaaca gtattgattc actatacagg
      541 gataactaaa ccatggcata gctgggctgg ttatccgtct gcatcatact ttaatatcgc
      601 gcgtgaacaa tctccctgga agaaatatcc tcttaaagag gcgcggactg ttgcagaaat
      661 gcagaaacaa tataagcatc tgtttgccca tggtgagtat atta
//
LOCUS       DAZOZY010000455          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZOZY010000455 DAZOZY010000000
VERSION     DAZOZY010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.458"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHURQ_004490"
     CDS             <1..>698
                     /locus_tag="ACHURQ_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826061.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAZOZY010000456          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZOZY010000456 DAZOZY010000000
VERSION     DAZOZY010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.459"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHURQ_004491"
     CDS             <1..>698
                     /locus_tag="ACHURQ_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR0826062.1"
                     /translation="VSCISPAKFHECFINWMRDCHSTDDKDVIAIDGKTLRHSYDKSR
                     RRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKD
                     IAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHGREEIRL
                     HIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKF
                     ATAIRNHWHVENNL"
BASE COUNT          230 a          131 c          163 g          174 t
ORIGIN      
        1 gtatcctgta tcagtcctgc aaaatttcac gagtgcttta ttaactggat gcgtgactgc
       61 cattctacag atgataaaga cgtcattgca attgatggaa aaacgctccg gcactcttat
      121 gacaagagtc gccgcagggg agcgattcat gtcattagtg cgttctcaac aatgcacagt
      181 ctggtcatcg gacagatcaa gacggatgag aaatctaatg agattacagc tatcccagaa
      241 cttcttaaca tgctggatat taaaggaaaa atcatcacaa ctgatgcgat gggttgccag
      301 aaagatattg cagagaagat acaaaaacag ggaggtgatt atttattcgc ggtaaaagga
      361 aaccaggggc ggctaaataa agcctttgag aaaaaatttc cgctgacaga attaaataat
      421 ccagcgcatg acagttccgc aatgagtgaa aagagtcacg gcagagaaga aatccgtctt
      481 catattgttt gcgatgtccc tgatgaactt attgatttca cgtttgaatg gaaagggctg
      541 aaaaaattat gcgtggcagt ctcctttcgc tcaataatag cagagcaaaa gaaagagccc
      601 gaaatgatgg tcagatatta tatcagttct gctgatttaa ccgctgagaa gtttgcgaca
      661 gcgattcgaa atcactggca cgtggagaat aatctgca
//
LOCUS       DAZOZY010000457          696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZOZY010000457 DAZOZY010000000
VERSION     DAZOZY010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.460"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..73)
                     /locus_tag="ACHURQ_004492"
     CDS             complement(<1..73)
                     /locus_tag="ACHURQ_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826063.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAF"
     gene            complement(83..>696)
                     /locus_tag="ACHURQ_004493"
     CDS             complement(83..>696)
                     /locus_tag="ACHURQ_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826064.1"
                     /translation="TEFDDNGKIIFNINALKYTKASYFEILGEKYIKITTSSPWLLEK
                     LGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFL
                     GDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDA
                     QCHLSEKLKYAIRDGLKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT          227 a          144 c          113 g          212 t
ORIGIN      
        1 tgaatgcagg gaaaaataat atggccataa aaaacatcga aagaaactct tttaatttaa
       61 catgtaaacg catggttaat cctcatatca cgggtggagt gttaagaaca tacataaatg
      121 gagtcatgtt ttcccttttc catttatcaa gttcctgttg ccgttttagt ccatctctaa
      181 ttgcatattt taatttttct gataaatggc attgagcatc gatttcattt aaaacaactg
      241 tacacactat ccctgtcagt aaaattgctc ctccgacaag aattactgga aagccaaagc
      301 taacacaggc aaaagaagcc aggcttccaa tagcggttgc aacaccaccg gctataactg
      361 ctttggccac atccattgaa aaatcgccaa gaaaattaac cagatctcgc tctgaactca
      421 taataaattc tatggttctc caggctactg aaaaccagat acagaactta actcctttta
      481 ttgattcaga taaagctcct cgccagccaa tagcgagttc cagaacttga ggcgctcgtg
      541 aagagaaaat atatttaccg agcttttcta aaagccaggg gctggatgtt gtgattttta
      601 tatatttctc tccaagtatt tcaaagtaac ttgcttttgt atactttaag gcattaatgt
      661 taaatataat tttcccattg tcatcaaact cagtca
//
LOCUS       DAZOZY010000458          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZOZY010000458 DAZOZY010000000
VERSION     DAZOZY010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.461"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHURQ_004494"
     CDS             <1..>695
                     /locus_tag="ACHURQ_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR0826065.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZOZY010000459          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZOZY010000459 DAZOZY010000000
VERSION     DAZOZY010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.462"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            149..>695
                     /locus_tag="ACHURQ_004495"
     CDS             149..>695
                     /locus_tag="ACHURQ_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR0826066.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          264 a          139 c           94 g          198 t
ORIGIN      
        1 taatctggaa gcgcaaaata acaaactgac aaaaattaat atttctgata gttataaact
       61 gaaatttctt aatcttgatt ataacaagct agcatcgctg gatgtctccc ggcaagaatc
      121 tctgattgag ttaagtgccc atcacaatat gatcactgac cttatattac acaatcatcc
      181 caggatgaaa acaatcactt taaacgacaa ccatattgca catttaaacg ccaaaaacac
      241 tacaaaactg gaatatttaa acttaagcaa taacaattta ctgccaacca atgacattga
      301 tcaactaata tcatcaaaac atctttggca tgtattagtt aacggcatca acaatgatcc
      361 acttgcacaa atgcagtact ggactgcagt aagaaatata attgatgaca ctaatgaagt
      421 gaccattgat ttatcaggac ttaatttaac cactcaacca ccagggctgc aaaacttcac
      481 atctatcaat cttgataata accaactcac acattttgat gcaaccaact acgatagact
      541 cgtaaagcta agtctgaata gtaatgctct tgagtcaata aattttcctc aaggcagaaa
      601 tgtaagtatt acacatatat ctatgaataa taatgctctc agaaatattg atatagatag
      661 gctttcatca gttacttatt ttagtgcggc acata
//
LOCUS       DAZOZY010000460          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZOZY010000460 DAZOZY010000000
VERSION     DAZOZY010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.463"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
BASE COUNT          241 a          105 c          143 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZOZY010000461          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZOZY010000461 DAZOZY010000000
VERSION     DAZOZY010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.464"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..683
                     /locus_tag="ACHURQ_004496"
     rRNA            <1..683
                     /locus_tag="ACHURQ_004496"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          157 a          151 c          230 g          146 t
ORIGIN      
        1 atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc tcctaaagag
       61 taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt agtgcaatgg
      121 cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca ggtcatagtg
      181 atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact ccggggataa
      241 caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc gatgtcggct
      301 catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat ttaaagtggt
      361 acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg tgggcgctgg
      421 agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc actggtgttc
      481 gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa gtgctgaaag
      541 catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag ggtcctgaag
      601 gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat gcgttgagct
      661 aaccggtact aatgaaccgt gagg
//
LOCUS       DAZOZY010000462          684 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZOZY010000462 DAZOZY010000000
VERSION     DAZOZY010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.465"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..560
                     /locus_tag="ACHURQ_004497"
     CDS             <1..560
                     /locus_tag="ACHURQ_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR0826067.1"
                     /translation="RFSQLESALNTQKNSIPALEKEVKALDKQMVAAQKAADAYWGKD
                     ANGKQMTREEAFKKIHQQRDEFNKQNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVP
                     IQQKRDFDINEQRRQTFLQSQKLSRKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMK
                     IDDINQANERWKKDTEQLRRNGVIK"
     gene            complement(616..>684)
                     /locus_tag="ACHURQ_004498"
     CDS             complement(616..>684)
                     /locus_tag="ACHURQ_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826068.1"
                     /translation="ALMMITYIPQISLFIPQLLGLM"
BASE COUNT          244 a          122 c          165 g          153 t
ORIGIN      
        1 atcgctttag tcagcttgaa tctgcgttga atacccagaa gaactcgatt cctgcgctgg
       61 aaaaggaagt gaaagcgctt gataagcaaa tggtggcggc gcagaaagca gcggatgcat
      121 attggggaaa agatgcaaac ggtaagcaaa tgacccgcga agaagccttt aaaaaaattc
      181 accaacagcg tgatgagttt aataaacaga acgatagcga ggcgttcgcg gttaaatatg
      241 acaaagaggt ttatcaaccg gcgattgcgg catgtcataa acagagtgaa gagtgttatg
      301 aagtgccgat tcagcagaaa cgagatttcg atatcaacga acaacggcga cagacttttc
      361 tgcaatcaca aaaactcagc cgtaaattgc aggatgactg ggtaacgctc gaaaaagggc
      421 aatatccgtt aacgatgaaa gtttcagaaa taaacagtaa aaaagtcgcg attttgatga
      481 aaattgacga tatcaaccaa gctaatgaac gctggaaaaa agataccgaa caactgcggc
      541 gcaatggggt gattaagtaa ctcatgttaa gtgcctttgc cggataatat aaaaagttat
      601 ccggcaaatt attttttaca ttagccctag tagctgtgga atgaacaagg atatttgcgg
      661 aatgtaagtg atcatcatca acgc
//
LOCUS       DAZOZY010000463          668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZOZY010000463 DAZOZY010000000
VERSION     DAZOZY010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.466"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHURQ_004499"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHURQ_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHURQ_004500"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHURQ_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          224 a          127 c          126 g          191 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgct
//
LOCUS       DAZOZY010000464          667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZOZY010000464 DAZOZY010000000
VERSION     DAZOZY010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.467"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHURQ_004501"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHURQ_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR0826069.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
BASE COUNT          150 a          140 c          167 g          210 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagat
//
LOCUS       DAZOZY010000465          665 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZOZY010000465 DAZOZY010000000
VERSION     DAZOZY010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.468"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
BASE COUNT          186 a          132 c          140 g          207 t
ORIGIN      
        1 aatccatttt acccagagtc ataaaataga gtgtctgttg atctcatttt catattcctt
       61 tcagactgaa gtgcgttttc atcacccgat gagaaagaac tgaagtgatg tttgccatag
      121 attgcgagat gccctgtctc attgactatc gcggtgcacc tttatacctg ttataccaga
      181 tcaaaaatca cgcaagccat acaacaaaac gagatttgca attcgtgtca caaaatatgt
      241 cgatcttttt ctaagaggaa gatgccatgt gaagccagac gaacacttga ggtggtcttc
      301 aaaaactaaa ggatttcttt taactgtttg ttttataaaa aatttattta gggtctaaac
      361 aagggaagct ttgcaagcta actcagtgag cgtggtgaaa acaagtgttt acccgccatc
      421 aggctgatca taattctcac catgaaatat gtttcctgat ttgtggcttg taactggtcg
      481 cttctgaagt cgatctagag aggcttgttg atgttggtgt tttcaggatg atgtttcact
      541 tagtttgttt gccgtatcgc ccggcgaata gctgtgattg aggaaggttt aagtcgcagt
      601 gaccaaagcc atatttaccc acgaatgtag atgaaaaagc gtctcctgcg ttccccctta
      661 tctcc
//
LOCUS       DAZOZY010000466          662 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZOZY010000466 DAZOZY010000000
VERSION     DAZOZY010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.469"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>662
                     /locus_tag="ACHURQ_004502"
     CDS             <1..>662
                     /locus_tag="ACHURQ_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR0826070.1"
                     /translation="TFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVPD
                     NSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLNY
                     SFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIH
                     GI"
BASE COUNT          201 a          128 c          145 g          188 t
ORIGIN      
        1 tacttttatt gcagatgata ataacgcaga actcatccca tgcctgagta cagatcttct
       61 tgtcagtctt ggtataaaaa aaatagcgtt attagacaat aaagaacatt cggcagaaaa
      121 acatgtgccg gacaacagcg catgcacacc acttcaggat cgtttggccg acgcatcaac
      181 tgagtttgat gttggtcagc aacatctgtc actctccgtt ccgcaaattt atgttggcag
      241 aatggctcgc ggctatgttt ctcctgatct gtgggaagaa ggaataaatg cagggcttct
      301 aaactatagt tttaatggta attctattaa taatcgtagt aaccataatg caggaaaatc
      361 aaactatgca tatttgaatt tacagagtgg catcaacatt ggtagttggc gactacgcga
      421 taactcaacg tggagttata acagtgggag tagcaattca tctgacagca ataaatggca
      481 gcatatcaat acgtcggctg aacgtgacat tattccctta cgctcacggt taacggtagg
      541 tgatagttat accgatggcg atatttttga tagtgtgaac tttcgtggcc tcaaaataaa
      601 ttcaacagaa gcgatgttgc ccgatagaca acatggtttc gctccggtga ttcatggtat
      661 tg
//
LOCUS       DAZOZY010000467          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZOZY010000467 DAZOZY010000000
VERSION     DAZOZY010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.470"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>650
                     /locus_tag="ACHURQ_004503"
     CDS             <1..>650
                     /locus_tag="ACHURQ_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0826071.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEA"
BASE COUNT          129 a          189 c          186 g          146 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc
//
LOCUS       DAZOZY010000468          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZOZY010000468 DAZOZY010000000
VERSION     DAZOZY010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.471"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHURQ_004504"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHURQ_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHURQ_004505"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHURQ_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          154 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaacg
//
LOCUS       DAZOZY010000469          650 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZOZY010000469 DAZOZY010000000
VERSION     DAZOZY010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.472"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHURQ_004506"
     CDS             <1..196
                     /locus_tag="ACHURQ_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0826072.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
     gene            complement(197..388)
                     /locus_tag="ACHURQ_004507"
     CDS             complement(197..388)
                     /locus_tag="ACHURQ_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR0826073.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(493..>650)
                     /locus_tag="ACHURQ_004508"
     CDS             complement(493..>650)
                     /locus_tag="ACHURQ_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR0826074.1"
                     /translation="TIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSD
                     AWIMRLA"
BASE COUNT          183 a          147 c          162 g          158 t
ORIGIN      
        1 ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg
       61 gcagggaatt acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt
      121 ggatattggc aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct
      181 tgtgcaattg caataattaa aaatcaccgt actcctgtgc aggtcgccag aaaccatcta
      241 taaaatcctc aatcggaaaa caaccgccat ggcggatccg ttgatcgctc atagaataaa
      301 gacactgctg ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt
      361 agcaaacaaa aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa
      421 gtttaggaga gattttacaa cgggggaata accaggacaa ataacccgcc atcccgacgg
      481 gttctttttg gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt
      541 acggtaaagg acagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca
      601 ggcatcgagt tgcatacata aatcggggtc agatttacaa ggaatggtta
//
LOCUS       DAZOZY010000470          644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZOZY010000470 DAZOZY010000000
VERSION     DAZOZY010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.473"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>644)
                     /locus_tag="ACHURQ_004509"
     CDS             complement(<1..>644)
                     /locus_tag="ACHURQ_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826075.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPA"
BASE COUNT          107 a          211 c          179 g          147 t
ORIGIN      
        1 gccggttcac cgttcaccgt gcgccgggtg atacggtccg acgcatcgta atgccagagg
       61 gtgataagcc cctcatcttc actctgcgtg agttttccgg tcaggtcata ctggtaacgt
      121 tgcgtccggc cgtcaaagcc gcgctgctcc gtcagcctgt cgaccgggtc ataccggaac
      181 gtggactggc tgccgttctc gttggtcagc acagtgatgc gcccggcggc gtcatacccc
      241 atgcttcgcg tcagaccgcc ctgcgtggtg ctgacggcct ttccccacgc atcatactgt
      301 atctcactgc ggctgccgtc cggggcaacg atagcggtga ggtctcctgc ggcgctgtac
      361 tcataacggg tttcacgccc ctgcgcatcc ctctgactga ccagttgacc gcgcgggtta
      421 taactgctgt aagtgctgat gccttcttcc cggtgaacgg cgatttgttg accgtaccgg
      481 tcatactcat accgcgtcgt gtaccccgag cagtccgtaa acgcctgaag ctgaccgtac
      541 cggtttcgcg ttattctccg cctcaccccg gttccgtctt ccaccgcaca gggcaggcca
      601 ctgcgggaag aatcataaaa ccagcgcgta atgttaccgt tgcg
//
LOCUS       DAZOZY010000471          643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZOZY010000471 DAZOZY010000000
VERSION     DAZOZY010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.474"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..194)
                     /locus_tag="ACHURQ_004510"
     CDS             complement(<1..194)
                     /locus_tag="ACHURQ_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR0826076.1"
                     /translation="MLVSKSNEFNTSSFIDSGNCNERKSSESMELLAYSIINLICKEA
                     ASGTYRGALETLQKMMSECI"
BASE COUNT          202 a          116 c          104 g          221 t
ORIGIN      
        1 tatatacatt cagacatcat tttttgtaaa gtttcaagag caccgcgata cgtccctgag
       61 gcagcttcct tacaaattaa atttataata ctataagcta gtagctccat ggattcagaa
      121 gattttcttt cattacaatt tccactatct ataaatgagc tagtgttaaa ttcgttgctt
      181 ttactaacta acattgtcta ttcctcagtt aatgtctaca tggctatttt taattttatt
      241 attgtttgtc actataaaaa atcgctcatt tgagacaatt gctggcatta acagcttcat
      301 atgctataca tggtactttt aattaaatta gcacaagaat gttaaattta ataaacaaaa
      361 agttatttcg ctgtatgata aaaaccaccc gttataattt attagtgaaa ttcgtttttc
      421 gagtgttaga aatttatatc taaatagcgt tgattaatga gcaaagccag actcctgtaa
      481 cgctcacaag actcatctac ctgcgacggg tattctggtc agtagtagat gtttaaggcg
      541 tggcagagac atttcatcct tactctacgg cattggcccg catacaaatc gtggttgtga
      601 cactcactta tcatcagtga gcgaacagag aatagttcag tga
//
LOCUS       DAZOZY010000472          641 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZOZY010000472 DAZOZY010000000
VERSION     DAZOZY010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.475"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>641
                     /gene="fliD"
                     /locus_tag="ACHURQ_004511"
     CDS             <1..>641
                     /gene="fliD"
                     /locus_tag="ACHURQ_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR0826077.1"
                     /translation="VSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDES
                     SDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLVL
                     TASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSSN
                     KITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          217 a          146 c          139 g          139 t
ORIGIN      
        1 aagtttccac cgaagcaggt gctgcccccg gtatctatac catcagcgta acccaactgg
       61 cgcaggcgca atctttacga actgattccc cgacaatcat tgcatcaaca aaagatgcac
      121 tgggtgatga gtcatcagat acacgcacaa taaaaattac ccaagacggt cgtaaagagc
      181 cgctggaaat caagctcaat aaagatcaga cgtcattgga tgagatcagc aaagccatta
      241 acgatgccga tagtggaatt tccgctagta tcgttaaagt caaagatggt aactatcaat
      301 tggtattgac cgccagtgaa ggtctggcca ataaaatgac catatctgtt gagggtgaca
      361 gtaaattaaa tgatctgctg gcttatgaca gcaagaccaa caccgggaat atgaaggaac
      421 tggttaacgc gcaaaatgca cagctaaacg ttaacggtat cgatattgaa cgttcaagta
      481 acaaaattac cgatgctcct cagggagtga ctctcgattt gaccaaaaaa gtcactgacg
      541 tccgggtcac ggtgacaaaa agcaacgata aagcaaccga agcaattaaa ggctgggtgg
      601 attcatataa ctccctcatt gacaccttta acaccctgac a
//
LOCUS       DAZOZY010000473          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZOZY010000473 DAZOZY010000000
VERSION     DAZOZY010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.476"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..607)
                     /locus_tag="ACHURQ_004512"
                     /pseudo
     CDS             complement(1..607)
                     /locus_tag="ACHURQ_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          190 a          160 c          145 g          145 t
ORIGIN      
        1 tactaccaat ccgattctgt acactatttc cgctgaaatt ataattgagc aagccggcat
       61 taataccggg atcccacaac tcaggaggaa tatatccacg cgcgcgatta ctcataaatg
      121 cctgagggat cgtcaggttg agtcgctgct gaccaacatc taaatgcgca gtagcgtcat
      181 ggatcattga ggttaatggc acgcaggcat catccaccag cagattcata ccggagacag
      241 aagccgtatt cagccccata ctggcaagct gcgcgcgtgt caggcaggga acaatccctt
      301 gttcactgtc gcccgtgtta aatgtgacat cacgcgttgc catataacca ttattcaaat
      361 agatatcgac gcgatacgtc cctggcggta attcttgccc attttcaaaa cgcgataaat
      421 cggccacagc ctagggatca tccgctaaaa agcgcggatt aaaatagagt tcggcagatg
      481 acaaaggtgc ctgtgcggca aaagcacagg cgacaaagag ccggacaaaa agaccagcca
      541 aacgatgctt acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat
      601 atgacattat tcctccggtt taaaatcacc cggcagtcaa
//
LOCUS       DAZOZY010000474          636 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZOZY010000474 DAZOZY010000000
VERSION     DAZOZY010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.477"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(64..615)
                     /locus_tag="ACHURQ_004513"
                     /pseudo
     CDS             complement(64..615)
                     /locus_tag="ACHURQ_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          171 a          169 c          104 g          192 t
ORIGIN      
        1 gctatgtgac ccccttggca aaaaagccaa atatcagcta catccatgta actattcagt
       61 aacttattta gcaagtctgt aataagttgc tctagagatt cctagctctt cctgaatctg
      121 caacctcgtt ttaccttgtg aaataagcat ctgtacagcc tctgaagaag cttttacagg
      181 tcttcccata tgtacccctt aagctttagc acgttcaata ccagcctttc tacgctctgc
      241 tatcaagctt ctttcaagct gtgccaacgt tgacataatg cctaacattg cttcacccat
      301 agcgcttgat aagtcaaatc cttctctaag gctgataact gtcacacctt tagcctgtag
      361 cgctttaacc gttgataaca catcaattgt gtcacgtcca agacggtcta cagctaccac
      421 aactacggtg tcaccttctc gtgcataagc taacaaactg ctgaaaccgt ttcttaaaga
      481 agctttgaca gaaccactga cagcttcatc agcgaaccat ttagcaatgt tgtaccctgc
      541 ctcttcaatt tgcatttgct ggttagtgat ggattggtca tctgtactca ccctgcaata
      601 tccaagtatc gccataatgt tccccttaga agtctc
//
LOCUS       DAZOZY010000475          631 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZOZY010000475 DAZOZY010000000
VERSION     DAZOZY010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.478"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>631
                     /locus_tag="ACHURQ_004514"
     rRNA            <1..>631
                     /locus_tag="ACHURQ_004514"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          177 a          137 c          197 g          120 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      481 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
      541 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
      601 gtgggaaggc ccagacagcc aggatgttgg c
//
LOCUS       DAZOZY010000476          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZOZY010000476 DAZOZY010000000
VERSION     DAZOZY010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.479"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHURQ_004515"
     CDS             <1..91
                     /locus_tag="ACHURQ_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826078.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHURQ_004516"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHURQ_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR0826079.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZOZY010000477          555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZOZY010000477 DAZOZY010000000
VERSION     DAZOZY010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.480"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            78..555
                     /locus_tag="ACHURQ_004517"
                     /pseudo
     CDS             78..555
                     /locus_tag="ACHURQ_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
BASE COUNT          136 a          121 c          123 g          175 t
ORIGIN      
        1 agctgataga aacagaagcc actggagcgc ctcaaaaaca ccatcataca ctaaatcagt
       61 aagttggcag catcaccgcc gttgtctaca catttattct ctcggtgctg atttaccggg
      121 aagtaaaatg gcgccattta cccaaactga ttctggagtc ggttgttacc acttcgattg
      181 tattactgtt gatcggtttt tccgtgggca tgtcctgggc aatgactaat gctgatattc
      241 cttatatgat cagcgacgca ttgatgggga tttctgataa cccactgatc atattgctgc
      301 tgattaatat cgtcttatta attgtcggca tctttatgga catgacaccg gctgtactaa
      361 tctttacacc gatcttcttg ccgattgcac aagagctggg aatggaccca gtacatttcg
      421 gtattatgat ggtggccaac ctttgtattg ggctgttaac accgccggtg ggtagtgcgc
      481 tatttgttgg ttgttctatc tcgggtgtga agattcaaca tttaatcaaa ccattgctgc
      541 cattttatgc tagca
//
LOCUS       DAZOZY010000478          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZOZY010000478 DAZOZY010000000
VERSION     DAZOZY010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.481"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            44..553
                     /gene="yfdF"
                     /locus_tag="ACHURQ_004518"
                     /pseudo
     CDS             44..553
                     /gene="yfdF"
                     /locus_tag="ACHURQ_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          223 a           98 c           86 g          146 t
ORIGIN      
        1 gtaatatata tttgtaacaa atcaatcaaa atggaataaa atcatgctac catctatttc
       61 aatcaacaat accagcgcaa cttacccaga atccattaat gaaaataaca atgatgaagt
      121 taatggatta gtacaagagc tcaaaaacct ttttaatggt aaggaaggaa taagcacctg
      181 tattaaacat ctacttgagc ttataaaaaa cgccatacga gtaaacgacg atccttaaga
      241 tttaatatta ataattcctc agttacttat attgatattg gctccaatga tacagaccat
      301 attactattg gtatcgacaa ccaagaacca atagaattac ctgcgaacta taaagacaaa
      361 gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga tatcaataac
      421 aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg ttttatcttc
      481 gataagatat cacacaaact cagacatacg gtaacggaat ttgatgagag cgggaatagc
      541 gaaccaacgg act
//
LOCUS       DAZOZY010000479          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZOZY010000479 DAZOZY010000000
VERSION     DAZOZY010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.482"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>553
                     /gene="feaR"
                     /locus_tag="ACHURQ_004519"
     CDS             <1..>553
                     /gene="feaR"
                     /locus_tag="ACHURQ_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR0826080.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          142 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aat
//
LOCUS       DAZOZY010000480          552 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZOZY010000480 DAZOZY010000000
VERSION     DAZOZY010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.483"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>552)
                     /locus_tag="ACHURQ_004520"
     CDS             complement(<1..>552)
                     /locus_tag="ACHURQ_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR0826081.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLDDAKVNLTPVIDSILETFLKSPY
                     INDVRILDWCFNKRMQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAETLF
                     FNLDKEPYKNSRKLQELIWDKLVA"
BASE COUNT          180 a           91 c           77 g          204 t
ORIGIN      
        1 tgcaaccaac ttatcccaaa tcaattcctg taatttacgg ctatttttat agggttcttt
       61 atcgagattg aaaaataagg tctcagctat tttaatttta gattgatcgc agctaaaatc
      121 tttagattga tcgcagctaa aatcaatatg atttatcacg gagcatgcat actttatctt
      181 ttcagaatca ccaaaatatt gcattctttt attaaaacac caatccagta ttcttacatc
      241 attaatatat ggggatttta aaaaagtctc cagaatcgaa tcgatgaccg gtgtaaggtt
      301 aacttttgca tcatctaagg aatcaataag aatatgagac aggttaactt tgatgtcatt
      361 aatttcattg tcagtagctt tcttattatt aagaagataa ttatttactg cgttaaggtc
      421 atctatttct ttcagtatcc ctttttcacc aagcaagtat gaaatcgatt cattacatac
      481 ataaggcatt aatagcagat gttctttcct tattgctaat aatgcattgt tcaatggttt
      541 ttctaataaa tc
//
LOCUS       DAZOZY010000481          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZOZY010000481 DAZOZY010000000
VERSION     DAZOZY010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.484"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHURQ_004521"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHURQ_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZOZY010000482          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZOZY010000482 DAZOZY010000000
VERSION     DAZOZY010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.485"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHURQ_004522"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHURQ_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          159 a          128 c          144 g          115 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgcca
//
LOCUS       DAZOZY010000483          541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZOZY010000483 DAZOZY010000000
VERSION     DAZOZY010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.486"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>541
                     /gene="espX1"
                     /locus_tag="ACHURQ_004523"
     CDS             <1..>541
                     /gene="espX1"
                     /locus_tag="ACHURQ_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR0826082.1"
                     /translation="LFFKHKGITLLRLDVFDDRTSECLSEEIKIYQECHEKFVNFLKA
                     NFNQEIYPQLYTPEIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHLNQEIKKLIKK
                     NIFPELYNEQCNKIPSSSIDDNQGIMWQNFKTSNSAYSKLCGKLSLFKSVPSRLIEKS
                     AYCSTENMATNKFNVVFSYC"
BASE COUNT          210 a           97 c           81 g          153 t
ORIGIN      
        1 ttatttttta aacacaaagg tatcacatta cttagattgg atgtttttga tgacagaaca
       61 agcgaatgtc tttctgaaga gattaagata taccaagaat gccatgaaaa attcgttaat
      121 tttttaaagg caaacttcaa ccaagaaata tacccacagc tatatactcc tgaaatattt
      181 tatgaggcct gcagaaatct ccagtcattt tatgatcatc aggaaacgtc acaaaacgca
      241 aaatacagtg caatagacaa gaaaaaaagt catcttaacc aggaaatcaa aaagcttatt
      301 aaaaaaaata ttttccctga gctatacaat gagcaatgca ataaaatccc ctcctcatcg
      361 atagatgata accagggaat aatgtggcag aactttaaaa ctagcaattc ggcatattca
      421 aaactatgtg gaaaattatc tttatttaaa agcgtgccca gtcggttaat agaaaaatca
      481 gcttactgct caactgaaaa catggccact aataaattta atgtcgtctt ttcttactgc
      541 g
//
LOCUS       DAZOZY010000484          534 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZOZY010000484 DAZOZY010000000
VERSION     DAZOZY010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.487"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..502
                     /locus_tag="ACHURQ_004524"
     CDS             <1..502
                     /locus_tag="ACHURQ_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFR0826083.1"
                     /translation="ISDPNEPKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGT
                     PCNQTGEDNCPDCGAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQH
                     NDSNNETGEDELNWKRQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSD
                     ERLPDF"
BASE COUNT          178 a          109 c          141 g          106 t
ORIGIN      
        1 gattagcgac ccgaatgaac cgaaatggat tcccggtcat catatctcct caaatgaagt
       61 caaaaaaacg gaaaatgaag tgcgacagac tgaagataaa cagcaccaga acagcgaacc
      121 ggaggaggga acaccctgca atcagactgg cgaggataac tgccctgact gtggcgcggt
      181 gatgggagac gcaacttatc aggaaacttt tgacgaaaaa aaaccagatg aagcacagga
      241 agaagagccg aagaaaacgg aaaaagctga tgatttgtta ccggagaacg ctggcagcga
      301 tcagcataat gatagcaata atgaaactgg cgaagatgaa ctcaactgga aaagacaggt
      361 gttgattgcg gcggtttacg gtttatgtgc gaatcctgcg tgcatagcct atgcgcctgc
      421 aatccctgac atcgcaatga tgattgccaa caagcttgaa aattttggag tgaccagtga
      481 tgagcgctta cccgattttt gatcgtattg aagaaatggc atgggctcgt cact
//
LOCUS       DAZOZY010000485          522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZOZY010000485 DAZOZY010000000
VERSION     DAZOZY010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.488"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>522)
                     /locus_tag="ACHURQ_004525"
     CDS             complement(<1..>522)
                     /locus_tag="ACHURQ_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826084.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTA"
BASE COUNT          143 a          141 c          133 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgaggaa cg
//
LOCUS       DAZOZY010000486          514 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZOZY010000486 DAZOZY010000000
VERSION     DAZOZY010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.489"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(116..424)
                     /locus_tag="ACHURQ_004526"
                     /pseudo
     CDS             complement(116..424)
                     /locus_tag="ACHURQ_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
BASE COUNT          139 a          132 c          125 g          118 t
ORIGIN      
        1 tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaatcaa
       61 catataacaa catcttaaaa aaaggcctga cattacgcca ggccttctgc gttaattaat
      121 cacgcttttc cagcagggtc cggtaaatca gaccaccgat aatgccgccg acaattggca
      181 ccacccagaa gaaccacagt tgttctaatg cccagccgcc ctggaagata gcaaccgcgg
      241 tgctgcgcgc cgggttaaca gaagtgttag tcaccggaat actaattaag tgaatcaggg
      301 ttaaggccag accaatagcg atcggcgcaa aacctgccgg cgcgaatttg tcggttgcgc
      361 cgtggatcac caacaggaaa cctgcactca ataccagttc aactaccagc gcggaggtga
      421 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg
      481 tttctatcag ctgtccctcc tgttcagcta ctga
//
LOCUS       DAZOZY010000487          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZOZY010000487 DAZOZY010000000
VERSION     DAZOZY010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.490"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHURQ_004527"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHURQ_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR0826085.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZOZY010000488          486 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZOZY010000488 DAZOZY010000000
VERSION     DAZOZY010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.491"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>486
                     /locus_tag="ACHURQ_004528"
     CDS             <1..>486
                     /locus_tag="ACHURQ_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR0826086.1"
                     /translation="PSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGV
                     KLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPS
                     ASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQLSRKVKFSNNWQKQKRK
                     I"
BASE COUNT          140 a          122 c          123 g          101 t
ORIGIN      
        1 cccgtcacag ccattgcaac agtcactgaa agaccttgag cgggcttaca aaaacttctt
       61 ccagaatcgg gctgcttttc cccgattcaa aaagcgggga cagaatgatg tattccgcta
      121 cccgcagggc gttaagctcg atcaggaaaa cagccgtatt tttctgccga aactgggctg
      181 gatgcgctac cggaacagcc gtcaggtcac gggtgttgtg aaaaatgtca ctgtcagcca
      241 gtcctgcggt aagtggtaca tcagtattca gacagaaagt gaagtatcaa ctccggttca
      301 cccttcagca tcaatggtcg ggctggatgc tggcgtggct aaactcgcca cgctgtcaga
      361 tggcacagtc tttgagcctg taaacagttt tcagaaaaac cagaagaagc tggcgagact
      421 tcagcgacag ttaagccgca aggtcaaatt cagcaacaac tggcagaagc agaaacgcaa
      481 aataca
//
LOCUS       DAZOZY010000489          485 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZOZY010000489 DAZOZY010000000
VERSION     DAZOZY010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.492"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHURQ_004529"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHURQ_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          138 a          120 c          105 g          122 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtatt
//
LOCUS       DAZOZY010000490          480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZOZY010000490 DAZOZY010000000
VERSION     DAZOZY010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.493"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            30..>480
                     /locus_tag="ACHURQ_004530"
     CDS             30..>480
                     /locus_tag="ACHURQ_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR0826087.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLVRSLRAGDLSAVYVPGIEDEAFRDLARAWASARDDLRHARQRLK"
BASE COUNT          117 a          116 c          123 g          124 t
ORIGIN      
        1 accccggtat ttccaccacg gaggccagca tgaacaacaa taacacattg tacgtcgggt
       61 tagatgttca caaagaatcg attactgtcg cttatgctat caattcagaa cctgttgaac
      121 tgatgggtaa aattggcaca tcacctactg atattcagaa tctttgtaaa cgtctcaggt
      181 caaagtcatc gcaggtcagt atcgtttatg aagcggggcc ctgtggttat ggactttatc
      241 gtcggctggt gaaatccggt tttgactgca tggtctgcgc cccctcactt atcccgaaaa
      301 aaccggggga gcgggttaaa accgatcgcc gtgatgccat cagacttgtg cgttcactgc
      361 gtgcaggaga tctttctgct gtctacgtac ccggcattga agatgaggca ttccgggatt
      421 tggcccgggc atgggcatct gcccgcgatg atttacggca tgcaaggcaa cgtctgaaat
//
LOCUS       DAZOZY010000491          476 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZOZY010000491 DAZOZY010000000
VERSION     DAZOZY010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.494"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>476
                     /locus_tag="ACHURQ_004531"
     rRNA            <1..>476
                     /locus_tag="ACHURQ_004531"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAZOZY010000492          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZOZY010000492 DAZOZY010000000
VERSION     DAZOZY010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.495"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            89..448
                     /locus_tag="ACHURQ_004532"
                     /pseudo
     CDS             89..448
                     /locus_tag="ACHURQ_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          113 a          113 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaacccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAZOZY010000493          467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZOZY010000493 DAZOZY010000000
VERSION     DAZOZY010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.496"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>467
                     /locus_tag="ACHURQ_004533"
     CDS             <1..>467
                     /locus_tag="ACHURQ_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR0826088.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALG"
BASE COUNT          119 a          108 c          152 g           88 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cactggg
//
LOCUS       DAZOZY010000494          461 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZOZY010000494 DAZOZY010000000
VERSION     DAZOZY010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.497"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..452)
                     /locus_tag="ACHURQ_004534"
     CDS             complement(<1..452)
                     /locus_tag="ACHURQ_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0826089.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFF"
BASE COUNT          145 a           89 c           71 g          156 t
ORIGIN      
        1 taaaaaaagt cgaaaagtaa agatccttca ttaaactctg tatttatagg tgagtaagta
       61 acaccatctt ttatataatg gccataaagc ccacaataaa tcgcatcccc tcccgtctca
      121 tgttgtctgg tatacatctt cagcagatag ttaggatcta tctcatcatc atcatccata
      181 aaacatataa actgtccttg cgccttgcgg atacctgtat tcctggcaga cgaaacaccg
      241 ccattttctt tattgataat aattaatcga gagtgggtta acccatactc tctcaaaacg
      301 tttgatgtgt tatctttaga accatcatta acaataacaa tttcaaaatt tgggtaagtt
      361 tgtttttcta atttcgttag agtttcagta atgtaatcag atgaattata cgtcggtatt
      421 attatggata ctaaagggta atcattgctc atcgtgttct c
//
LOCUS       DAZOZY010000495          456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZOZY010000495 DAZOZY010000000
VERSION     DAZOZY010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.498"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..386
                     /locus_tag="ACHURQ_004535"
     CDS             <1..386
                     /locus_tag="ACHURQ_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR0826090.1"
                     /translation="KGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDVY
                     NSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNIIC
                     SECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          133 a           96 c          122 g          105 t
ORIGIN      
        1 ttaaagggca ggttgttgaa cttgtacgtc tgagcggtcg cgagacgttg cggcaactgg
       61 aagccaagac aggtgcgaca agatatctga tgagtgttct cgccagagag ctggttgcca
      121 gtggcgatgt atacaactct ggttacgggt tattcccgtc tgaacaggcg cgtaaggact
      181 ggcaaaacgc ccgcaaaaaa ctctcaaggg caaatctgaa gaaaccatct gtggttgatc
      241 ctgaccttat ctggtcatta ccagacggag aaatacgccg ctacgacagg cgtcaaaaca
      301 taatctgtag cgagtgccgg aagagcgaag ctatgcagcg tgtactggct ttctatcagg
      361 gtaattttca gaaggtgctg ttgtgagcca aattaacaat cggaacttcg tgaagagaaa
      421 gcataatcca aaatctgaat aattaagttc agcact
//
LOCUS       DAZOZY010000496          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZOZY010000496 DAZOZY010000000
VERSION     DAZOZY010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.499"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHURQ_004536"
     CDS             <1..>442
                     /locus_tag="ACHURQ_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR0826091.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZOZY010000497          441 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZOZY010000497 DAZOZY010000000
VERSION     DAZOZY010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.500"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>441)
                     /locus_tag="ACHURQ_004537"
     CDS             complement(<1..>441)
                     /locus_tag="ACHURQ_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endochitinase"
                     /protein_id="HFR0826092.1"
                     /translation="ALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWGFSTNCPGKAK
                     ANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATPAQG
                     TVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAK"
BASE COUNT          114 a          109 c           95 g          123 t
ORIGIN      
        1 tttcgcatca cccggacagt tagaagaggc tacccaccaa gcatttttat ataccgcacc
       61 attaaagacg acataccagg tctgaccgcc ctgctgttta ttccaggcaa ctacagaaga
      121 attagacgga acagtgccct gtgctggtgt agccgaaccg ccattagccg gcgtattgga
      181 tgcaggcgta tttgaatttg aagaagatga gctgccgctc ttttcgcagg aaagtgtatt
      241 gccaaactga ctaatttcag cagctgttgc ggtacgcttt aaacgccacg ggttagttgc
      301 atcatttgct ttcgcttttc ccgggcaatt tgtagaaaaa ccccaccagg cattttcata
      361 aattttgcca tcgaaaataa cctgatattt attaccacct tgttggttat tccatgcttc
      421 cattgccaat gctggcagag c
//
LOCUS       DAZOZY010000498          438 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZOZY010000498 DAZOZY010000000
VERSION     DAZOZY010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.501"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(12..368)
                     /locus_tag="ACHURQ_004538"
     CDS             complement(12..368)
                     /locus_tag="ACHURQ_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR0826093.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
BASE COUNT          158 a           91 c           93 g           96 t
ORIGIN      
        1 caattctaag attaatttga accaaacttc ggatcgtttc cgtaacgatt gctgccagaa
       61 gtaccttctg tgcagaaaaa aaccagaagc acgagccagc caataaacgg aacaaaaaag
      121 agcaaggccc aggcacctga acggtcggta tcgtgtaagc gacggatagc aagcgcaagg
      181 acgggaagga aggttgccag caaataaagc atagacagat atggaagctc cagacctaaa
      241 atcaattgaa tgacattgat aatagcgccg acgatggcgt taatcagagt aaacatccag
      301 tactctttac gacgtgcgcg accagagaaa ccaacataat tttttaatac ggataaatac
      361 cactgcataa aaacatccct ttaattaaaa gtgacgaaag tctattccgg gtattgtgta
      421 aatgctattc gtatcttt
//
LOCUS       DAZOZY010000499          435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZOZY010000499 DAZOZY010000000
VERSION     DAZOZY010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.502"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..435
                     /locus_tag="ACHURQ_004539"
                     /pseudo
     CDS             1..435
                     /locus_tag="ACHURQ_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          124 a           86 c           96 g          129 t
ORIGIN      
        1 gattaataaa tttaataatt accttttccg ctggcagggc ctggatggct cagaaattct
       61 gatgcataac tttcctgaag acacttatga tagccgagca cgtgctcgct ctttagaata
      121 tattgaacaa aactataatg aaaaagagat ctgtccctat gcgcttatgg tgtatggtgt
      181 atggtgtcgg tgatggcggc gctggccccg gtgaggaaca tatagaacga ttaacacgta
      241 tacgcaatat tgatggctta cctcatgttg atttcagccg tgttgataag ttcttcactt
      301 acgccgacgc ctttcgcgaa agcttgccca ttatttcagg tgaactttat ttcgaggctc
      361 atcaagggtg ctttacatca gagtcagcaa ctaaagctca taatcgaaat atggaaaata
      421 aattacatga tgctg
//
LOCUS       DAZOZY010000500          435 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZOZY010000500 DAZOZY010000000
VERSION     DAZOZY010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.503"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>435
                     /locus_tag="ACHURQ_004540"
     CDS             <1..>435
                     /locus_tag="ACHURQ_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR0826094.1"
                     /translation="HIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKALA
                     EKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGVC
                     GGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           84 c          123 g          120 t
ORIGIN      
        1 ggcatattgc tgccaatatt cagtttccac caaattttga tgagtcaaaa cagtatccgg
       61 cgattatcag cgtccatcct tttggcagtt gtaaggagca gacgtctggc aatatctatg
      121 gcaaggcatt ggctgaaaaa ggctatctcg ttctcgctta tgacgccagt ttccagggag
      181 aatctggtgg ggagcctcgc tggatagaag atccgacaca gcgcgtagag gatattagcc
      241 gtgttatcga ttatgcagta actctgccat atgttaatgc tgaacgtatt ggcgtgctgg
      301 gagtgtgtgg gggtggtggc tatgccatta atgccgcgct gacagaaaaa cgaattaaag
      361 cagtagtgag tattacgggt gttaatatcg ggcgtctttt tcgggaaggt ttcagcaatt
      421 acgatccgat tggcg
//
LOCUS       DAZOZY010000501          434 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZOZY010000501 DAZOZY010000000
VERSION     DAZOZY010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.504"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHURQ_004541"
     CDS             <1..187
                     /locus_tag="ACHURQ_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFR0826095.1"
                     /translation="RPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIW
                     DMGRIAMSVAQQPQCHK"
BASE COUNT          132 a           99 c          107 g           96 t
ORIGIN      
        1 ccgccccgtc attgacagag catgggatgc tcaactcagg ctttgtaaga ggtatcgaaa
       61 acttcaggcc aaaggaaaga atgtcaatat tacaattgtt gctgttgcac gtgagctggc
      121 gggttttatc tgggatatgg gccgaatagc aatgtctgtc gcacaacaac cacaatgtca
      181 caaataaaaa atgagaataa ccagttccct gaaggcgacg tagcccggta caagagtaac
      241 ccacgttagc gcttggcgac ggcggaacaa agctgattcg cgacgtaaga aagtggcagg
      301 ctcatatgac ggacctctgt aatgcggtat ccaacccgcg aatatcagcg tgattcaccg
      361 tcggatttac tgctacgtcg ccctcaggga attaaaccta aaaagaaaat cgtcaaaatt
      421 agagattccg gttt
//
LOCUS       DAZOZY010000502          429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZOZY010000502 DAZOZY010000000
VERSION     DAZOZY010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.505"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>429
                     /gene="espX1"
                     /locus_tag="ACHURQ_004542"
     CDS             <1..>429
                     /gene="espX1"
                     /locus_tag="ACHURQ_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR0826096.1"
                     /translation="FCCVLTNISCFESDLSNTIFSNGEINNLFIKKSNIFGTSFTNTM
                     IKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDN
                     VKLNNDVYKELNKFMHDYDKTSSKMRKYLQSINECMLLIK"
BASE COUNT          162 a           78 c           66 g          123 t
ORIGIN      
        1 atttttgttg tgtattaacc aatatatcat gttttgaaag cgacttatca aacaccatct
       61 tttcaaatgg agaaataaac aacctcttca ttaaaaaaag caacatattt ggaacatcgt
      121 tcactaatac catgataaaa aatttgcgct gtgaagatat tatgcctggc cgatggacca
      181 ctcaacttgt caataagcac ttaggttaca gatacacagg agtattcaaa accctggcaa
      241 gtattgatga caaaccgtcc cgtttcgaaa ttttgatacc attggtacaa actttagttc
      301 gcgataatgt aaagctaaat aatgatgtgt ataaagaact taataagttt atgcacgatt
      361 acgacaaaac atcatcaaaa atgagaaaat accttcagag catcaacgag tgcatgctcc
      421 ttataaaaa
//
LOCUS       DAZOZY010000503          427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZOZY010000503 DAZOZY010000000
VERSION     DAZOZY010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.506"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..411
                     /locus_tag="ACHURQ_004543"
     CDS             <1..411
                     /locus_tag="ACHURQ_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0826097.1"
                     /translation="AERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFAYALNQWPA
                     LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGALLYSLIGTCKLNGV
                     EPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT          108 a          113 c          126 g           80 t
ORIGIN      
        1 gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg gctgcgtgaa
       61 aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata tgccctgaac
      121 cagtggccgg cgctgacgta ctatgcagat gatggctggg ctgaggcgga caataacatc
      181 gctgaaaatg cgttgcggat ggtcagtctg ggccgcaaaa actacctgtt cttcggttcg
      241 gatcatggag gagagcgggg agcgctgctg tacagcctga tcgggacgtg caaactgaac
      301 ggagtggagc cagaaagcta cctccgctat gtccttgacg tcatagccga ctggccgata
      361 aaccgggtcg gcgaactgct cccctggcgc gtagcactgc cgactgaata acacattccc
      421 gtcaata
//
LOCUS       DAZOZY010000504          425 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZOZY010000504 DAZOZY010000000
VERSION     DAZOZY010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.507"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHURQ_004544"
     CDS             <1..314
                     /locus_tag="ACHURQ_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR0826098.1"
                     /translation="QLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNT
                     ELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLA
                     G"
     gene            complement(357..>425)
                     /locus_tag="ACHURQ_004545"
     CDS             complement(357..>425)
                     /locus_tag="ACHURQ_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826099.1"
                     /translation="GGTDEIMIYVAGRQILKDYQNK"
BASE COUNT          114 a           93 c           87 g          131 t
ORIGIN      
        1 aacagttatc gaaaaaattt cgtgccagcg tcagttatat ttgccatgaa tttaccaaag
       61 agtatcgtat ttcccctatt aactatgtta ttcaacggcg tatgacggaa gcaaaatggt
      121 cactcactaa tactgaatta tcacaggcag agatctcctg gcgtgtgggt tatgaaaatg
      181 tcgatcactt tgccaaactg tttttgcgcc atgtcggttg ttcgcccagc gattaccgta
      241 ggcaatttaa aaattgtttt gcggaacaag aaatcttatc tgaatttcct caaccggtaa
      301 gtcttgccgg ataaatctgg cggcgcactt tgttaaaaag ctgcgccgcc tgcagattat
      361 ttgttctgat agtctttcag gatctgccga cctgctacgt aaatcataat ttcgtctgta
      421 ccgcc
//
LOCUS       DAZOZY010000505          424 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZOZY010000505 DAZOZY010000000
VERSION     DAZOZY010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.509"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>424
                     /gene="istB"
                     /locus_tag="ACHURQ_004546"
     CDS             <1..>424
                     /gene="istB"
                     /locus_tag="ACHURQ_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR0826100.1"
                     /translation="YIIHRKNLLITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRL
                     LEQLAQGRVDGSWLKYLKQLQKIQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTI
                     VVSQFPVDKWHGLMENPTTADAILDRLVHNSHRVVLQG"
BASE COUNT          131 a           92 c          118 g           83 t
ORIGIN      
        1 actacataat ccacaggaaa aacctactga taaccggtcc gacaggatgc ggtaagagct
       61 ggatagccaa tgcgctgggc gaacaggcat gtcgacagaa atacagcgtc cggtactgta
      121 gaaccgggcg attactggaa cagttggctc agggacgtgt tgatggcagt tggctgaaat
      181 atctgaaaca gttgcaaaaa atacaggttc taatcctgga cgatctgggg ctggaacagt
      241 taagtaacgc ccagtgtaat gaccttctgg agatcacaga ggatcgttac ggacaaagca
      301 gcacgatagt ggtaagccag ttcccggtag ataaatggca tggtctgatg gagaacccaa
      361 caacagcaga tgccatcctg gacagactgg tacataactc acacagagtg gtccttcagg
      421 gaga
//
LOCUS       DAZOZY010000506          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZOZY010000506 DAZOZY010000000
VERSION     DAZOZY010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.510"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHURQ_004547"
     tRNA            41..116
                     /locus_tag="ACHURQ_004547"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZOZY010000507          398 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZOZY010000507 DAZOZY010000000
VERSION     DAZOZY010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.511"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>398
                     /locus_tag="ACHURQ_004548"
     CDS             <1..>398
                     /locus_tag="ACHURQ_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR0826101.1"
                     /translation="QSKSLGESLGGLMSDMSHGVDSLTDISYRVRAFFDEVTLAYRSL
                     NDTQKAVADGLANGLLSALKGLAGIVAVRSGIGAVGGIWNLIRAISTLGNVANTAAGR
                     INSRSTGSVEGGKSRLSVADAITRVMIVGM"
BASE COUNT          105 a           64 c          110 g          119 t
ORIGIN      
        1 tcaatctaaa agtcttggtg aatctcttgg tggcttgatg agcgatatgt cgcatggggt
       61 cgattcacta accgatatta gttacagggt acgagcgttc tttgatgagg tgacattagc
      121 ttatcgttca ctaaatgaca ctcaaaaagc tgtagctgat ggtcttgcaa atggcctgtt
      181 aagtgcatta aaggggcttg ctggtatagt cgctgtacgg tctgggattg gtgctgtagg
      241 tggtatctgg aacttaatta gggccatttc cacgcttgga aatgtggcaa atacagcagc
      301 aggaagaatc aattcacgtt ctactggtag tgttgaaggt ggaaaaagta ggctatctgt
      361 agctgatgca attaccagag taatgattgt cggtatga
//
LOCUS       DAZOZY010000508          396 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZOZY010000508 DAZOZY010000000
VERSION     DAZOZY010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.512"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..231
                     /locus_tag="ACHURQ_004549"
     CDS             <1..231
                     /locus_tag="ACHURQ_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR0826102.1"
                     /translation="KVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFRWI
                     TNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
BASE COUNT          127 a           85 c           77 g          107 t
ORIGIN      
        1 aaggttgtaa atgtaagatt ttcaaaaaat aaatgcctta tagaagccac cttaaaagga
       61 ttcaaatcag gagagttatc attttacgaa tacaggaaaa atcttgctac agcaggggtt
      121 ttcagatgga ttacaaatat ccacgaaaac aaaaggtatt actatacctt tgataattca
      181 ttactcttta ctgagaacat acagaacact acacaaatat ttccgcacta aatcataacg
      241 tccggtttct tccgtgccag aaccggactc gctggcatga tgaaatatgt gtacccggta
      301 accccggtgt gcatcgtttt tgattattcc cgcacactcg cgcagaagga gttccccgtc
      361 gggctacggt ctctgttaat acgggaatac ggcgac
//
LOCUS       DAZOZY010000509          387 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZOZY010000509 DAZOZY010000000
VERSION     DAZOZY010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.513"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..387)
                     /locus_tag="ACHURQ_004550"
                     /pseudo
     CDS             complement(1..387)
                     /locus_tag="ACHURQ_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          122 a           85 c           52 g          128 t
ORIGIN      
        1 ggtaaatgca gtatgcattt tttgtcattc aagcaactat caataaattc ttttttaaaa
       61 gacaaaaaac tccatgataa aaaaccaccc tgcccctgaa ttttgttgtc agattgtaga
      121 ttaacgccaa ataacgtctt tacatcttcc ctgagattat agaatgccgt atcaacaaga
      181 gcctcgatcg tttgtttatc aacaatttta ataaactctt cgtatacttg cttttgcgtc
      241 ttatggccgc ccagcgccca tgaatactca tgttctttta aaaccagtat ctaaatcaga
      301 tattcccggc gtatttatct gttctggctg gatactcata ttttcatcat ctaaacaata
      361 ctcagaaaaa atatcatcac acgcagt
//
LOCUS       DAZOZY010000510          385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZOZY010000510 DAZOZY010000000
VERSION     DAZOZY010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.514"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>385
                     /locus_tag="ACHURQ_004551"
     CDS             <1..>385
                     /locus_tag="ACHURQ_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFR0826103.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKN"
BASE COUNT          110 a           79 c           93 g          103 t
ORIGIN      
        1 tcatgacaat gaaaccgagt tgatttacgt caagaaaggg gtagcaaggt ttaccatcga
       61 ttcttcgtta tatgtcgcgc acgccgatga cattgtggtg atagaacgtg gcaggctgca
      121 tgcggtggcc tctgacgtta acgatccagc aacgacgtgt acctgtgcgc tgtacggctt
      181 tcagtttcag ggggctgagg aaaatcagct actgcaaccg cattcttgcc cggtaattgc
      241 cgcagggcag ggaaaagaag tcattaaaac cttatttaat gagctaagtg tgattttgcc
      301 gcaaagtaaa aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat
      361 tctttactac gaaaacttta aaaat
//
LOCUS       DAZOZY010000511          372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZOZY010000511 DAZOZY010000000
VERSION     DAZOZY010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.515"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(212..>372)
                     /locus_tag="ACHURQ_004552"
     CDS             complement(212..>372)
                     /locus_tag="ACHURQ_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR0826104.1"
                     /translation="VKLSMPELEELAAAMVQAVVERNDEIYRRQREMKEELSSLDDLA
                     SIRAFDVK"
BASE COUNT           87 a          128 c           74 g           83 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacagaggc ccgcccccgg gcctccccgg attcatccgt ttctctgttc
      121 agcctgaccg cacaccccgg cggccggatg acagactccg cctcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attacttaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagactgctc agctcctctt tcatttcacg ctgacggcga taaatctcgt
      301 cattgcgttc aacaacagcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ct
//
LOCUS       DAZOZY010000512          366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZOZY010000512 DAZOZY010000000
VERSION     DAZOZY010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.516"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>366
                     /locus_tag="ACHURQ_004553"
     CDS             <1..>366
                     /locus_tag="ACHURQ_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFR0826105.1"
                     /translation="FFITITNNMTSILRSEFDEIWKAMLTLQFHDILPGSCISRVYHE
                     TEKEYLKLEAKTEKIISDAQSTLLSKIDTSSYKDPHILFNTTCFARNEWININNNWLK
                     ARVNSYGYAVIVPKNKIVNG"
BASE COUNT          126 a           64 c           59 g          117 t
ORIGIN      
        1 attttttata actattacta ataatatgac atcaattctt cgcagtgagt ttgatgaaat
       61 atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat gtatttcaag
      121 agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg agaaaattat
      181 ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata aagatccgca
      241 cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa acaacaactg
      301 gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga ataagatcgt
      361 aaatgg
//
LOCUS       DAZOZY010000513          357 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZOZY010000513 DAZOZY010000000
VERSION     DAZOZY010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.517"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>357
                     /locus_tag="ACHURQ_004554"
     rRNA            <1..>357
                     /locus_tag="ACHURQ_004554"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           95 a           82 c          105 g           75 t
ORIGIN      
        1 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
       61 gccggaaggt taattgatgg ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa
      121 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      181 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
      241 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
      301 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtg
//
LOCUS       DAZOZY010000514          353 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZOZY010000514 DAZOZY010000000
VERSION     DAZOZY010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.519"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..353
                     /locus_tag="ACHURQ_004555"
                     /pseudo
     CDS             1..353
                     /locus_tag="ACHURQ_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT          122 a           57 c           63 g          111 t
ORIGIN      
        1 gaagagttta ccttagtaaa gaaaaggaag tatccattaa ggtggtatat tttataaata
       61 atgtcgccgt ccataataat actatcgaaa ttccacagac agtaaatggt ggttacgatt
      121 tttcacacct tagcctgaaa ggtatcgtga ttaaagatga agatttatcc aattcgaatt
      181 ttgcaggttg cagactacaa aacgctattt tccaggactg taatatgtat aaaacgaatt
      241 tttattacgc cataatggaa aaatactttt tgataattgt attctcgatg actcaaattt
      301 cgctcagata aaaatggccg acggaactct aaatgcatgc tccgttatgc atg
//
LOCUS       DAZOZY010000515          349 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZOZY010000515 DAZOZY010000000
VERSION     DAZOZY010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.521"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>349
                     /locus_tag="ACHURQ_004556"
     CDS             <1..>349
                     /locus_tag="ACHURQ_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR0826106.1"
                     /translation="PINKKRIIHSVPGGTWKDWPKELVAACHMKSSGKGYASVYGRMS
                     WDKPSPTITTLCYGFGNGRFGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSI
                     GKMIGNAVPVELGK"
BASE COUNT          106 a           53 c           87 g          103 t
ORIGIN      
        1 tccgattaat aaaaaaagaa ttatacactc tgttcctggt ggtacatgga aggactggcc
       61 gaaagagttg gttgccgctt gtcatatgaa atccagcggt aaagggtatg ctagtgtata
      121 tgggcgcatg tcttgggata agccaagtcc aacaattaca actctttgtt atggtttcgg
      181 gaatgggcgt tttggacatc cagaacagca tagagcgatt tctcttagag aggctgcttt
      241 attgcaaact ttcccgatgg attatatttt tgtagaagac aaaaacaagt ttgttataag
      301 aagtattggg aaaatgatag gtaatgctgt tcctgtggaa ctaggaaaa
//
LOCUS       DAZOZY010000516          344 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZOZY010000516 DAZOZY010000000
VERSION     DAZOZY010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.522"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
BASE COUNT          119 a           42 c           84 g           99 t
ORIGIN      
        1 ttctttattt tttaaaagtg gaaaggtaaa agataaggta aacaaatgga ttaaagtaat
       61 gtgtgattta ttttggggga taggatagtg gtgtgatatg gataaatata aattacagtg
      121 gctggcatat ttcgacctga cgccggagta gataaaaaaa cagacaggat tggagtatag
      181 caagcgtctg acaatctctc gggacagtag tatgatgaag aggcggggtt gtactataac
      241 cgtaaccggt actacgatcc gctaaagggc aagtatatga ctcagggaaa gatcatcaga
      301 agttcacttt ttgtactaaa taattcgcat tttatgttta aaaa
//
LOCUS       DAZOZY010000517          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZOZY010000517 DAZOZY010000000
VERSION     DAZOZY010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.523"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..292
                     /locus_tag="ACHURQ_004557"
                     /pseudo
     CDS             1..292
                     /locus_tag="ACHURQ_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          125 a           51 c           66 g          100 t
ORIGIN      
        1 tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggata
       61 aatgtcgaat aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc
      121 cgttcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga
      181 agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta
      241 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt tttaacatcg
      301 actggtaaac aggaattgtt atttagataa atatggcgat gc
//
LOCUS       DAZOZY010000518          341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZOZY010000518 DAZOZY010000000
VERSION     DAZOZY010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.524"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..319
                     /locus_tag="ACHURQ_004558"
     CDS             <1..319
                     /locus_tag="ACHURQ_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFR0826107.1"
                     /translation="GISYGTFNKKKIFSGNIDREEIKEKSKIYGFSTHSDYTHTKHGE
                     KLATVKQHRNDLSHGNVSFAEIGKNVSYQDLENISLEVIAYLDAIVNNIEHYINNNEY
                     LEQ"
BASE COUNT          135 a           49 c           56 g          101 t
ORIGIN      
        1 cggtatatct tatggcacgt tcaacaaaaa gaaaatattc tcaggaaata ttgatagaga
       61 ggaaatcaaa gaaaaatcga aaatatatgg tttttctaca cattccgatt acactcacac
      121 taaacatggt gaaaaattag ccactgttaa acaacatcgt aatgatttat ctcatggcaa
      181 tgtatcattt gctgagattg gaaaaaatgt ttcatatcag gacttagaga atatttcatt
      241 agaagtaatc gcctatcttg atgctatcgt gaataatata gagcactata taaataataa
      301 tgaatattta gaacaatagt tataaggcct gtaaagcagg c
//
LOCUS       DAZOZY010000519          340 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZOZY010000519 DAZOZY010000000
VERSION     DAZOZY010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.525"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>340
                     /locus_tag="ACHURQ_004559"
     CDS             <1..>340
                     /locus_tag="ACHURQ_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826108.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPEG"
BASE COUNT           82 a          124 c           88 g           46 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaaggg
//
LOCUS       DAZOZY010000520          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZOZY010000520 DAZOZY010000000
VERSION     DAZOZY010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.526"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(1..335)
                     /locus_tag="ACHURQ_004560"
                     /pseudo
     CDS             complement(1..335)
                     /locus_tag="ACHURQ_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           86 a           72 c           57 g          120 t
ORIGIN      
        1 gaaattattt cttttacatt gtcgcgtcta aattttgcat cgtccacttg aaaattatat
       61 cttattgctc gacctaaacg tttaaaaact tcgttaggtt ctctcagtct ttccccgttg
      121 agctcgttta caacctgatg atcggaacct gttgcaaatg attctgccat gatagcttta
      181 ctgctttcaa accacgctga agcattgctg aagcattcct ggctttttca ctacaatatt
      241 gggtgagact atcaccatta atacgaattc ctctttgaat gtggtagtcg tcctgatcgt
      301 agtaaccgag agataagtaa tcatttccgt tttta
//
LOCUS       DAZOZY010000521          335 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZOZY010000521 DAZOZY010000000
VERSION     DAZOZY010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.528"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(13..>335)
                     /locus_tag="ACHURQ_004561"
     CDS             complement(13..>335)
                     /locus_tag="ACHURQ_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826109.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
BASE COUNT          100 a           77 c           79 g           79 t
ORIGIN      
        1 ttattattct gcttaatcaa cattgctggc tggcgtcgct tcaatatggc gctcttcagc
       61 cgttaatccc tttatatatt tactgtattt ccaccacgca aagccaagga agataacaat
      121 accgccgacg ttataaaaaa tgatggtcag aatattcgcg ccagtcggga atgtcgaagc
      181 gacaaaccct acggcaaata tcgcaatcag cattgaaaca acgataatac cggtgcggcg
      241 ggagcccata cggaaatcgc gtggcaggtg atctaatttg gcgcgtaaat tcaggtaagc
      301 cagcatgatg aataacggcg gaagcatgga cgctg
//
LOCUS       DAZOZY010000522          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZOZY010000522 DAZOZY010000000
VERSION     DAZOZY010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.530"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..332
                     /locus_tag="ACHURQ_004562"
                     /pseudo
     CDS             1..332
                     /locus_tag="ACHURQ_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           85 a           83 c           99 g           65 t
ORIGIN      
        1 gctatgcagc gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca
       61 tcatccggat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca
      121 gcgcgtatac gatgaaaatc acaaggtata cggtgtaaag tctggcgtca gttgttacgg
      181 gaaggtatca gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc
      241 ggtgttctcc ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc
      301 gaccgcgtaa accgtcagtt cgtggcagaa cg
//
LOCUS       DAZOZY010000523          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZOZY010000523 DAZOZY010000000
VERSION     DAZOZY010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.531"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHURQ_004563"
                     /pseudo
     CDS             complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHURQ_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          112 a           63 c           57 g          100 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaagttc taagagagca cggattccct gtcattaata
       61 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
      121 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
      181 tgactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
      241 cattgaaaaa ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta
      301 acctaataaa attatgctgt gtatagtcta aa
//
LOCUS       DAZOZY010000524          330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZOZY010000524 DAZOZY010000000
VERSION     DAZOZY010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.532"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            50..>330
                     /locus_tag="ACHURQ_004564"
     CDS             50..>330
                     /locus_tag="ACHURQ_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826110.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQ"
BASE COUNT          124 a           49 c           53 g          104 t
ORIGIN      
        1 atgtattgtt tattttgtta ctaacaagta aaaatattac aggttattta tgaaagtatt
       61 tcacaatata tttaagcact tctcttcaaa ccatcaagat aagcattcag ataaagttaa
      121 cagtcatcaa caccatggca aggttgataa aaaatatcga tcaaacacag ttgaagttaa
      181 tatgctgaat gacttttcgc aaatagataa cgatttaggc ctgaatatca tctatttcct
      241 gcaacatggt cactggaggg taaatgaaca tagtcttcag atggagaaaa tatggtttta
      301 caatagtgag ccatctatag atattcaaga
//
LOCUS       DAZOZY010000525          326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZOZY010000525 DAZOZY010000000
VERSION     DAZOZY010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.533"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHURQ_004565"
     CDS             complement(<1..242)
                     /locus_tag="ACHURQ_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0826111.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           97 a           62 c           70 g           97 t
ORIGIN      
        1 ttaatggata aatccatcaa tttgtggaca ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcgt cattgataat ctggaatgcg
      121 acatcgtcgc gcatttcgtg cagcggaaaa cgttttgatt ctgcgatagt ggaaatagac
      181 ttcgcaccaa aacgtgaatc gagtagttcc gaccttaaat ccgttacttg cttcttatcc
      241 attttaaact ccttaaaatg attggatcgc attaaaaaag taggatttat cgataaagta
      301 agcaagtgca aaggactcgt gtttaa
//
LOCUS       DAZOZY010000526          313 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZOZY010000526 DAZOZY010000000
VERSION     DAZOZY010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.534"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>313)
                     /locus_tag="ACHURQ_004566"
     CDS             complement(<1..>313)
                     /locus_tag="ACHURQ_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR0826112.1"
                     /translation="QLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNY
                     RENSLMNSCRTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLW
                     NV"
BASE COUNT          101 a           54 c           48 g          110 t
ORIGIN      
        1 gaacattcca taaataacga gttttatgat aagctctatt ttgactcaat aatgtatgaa
       61 tttcttcttt cctgttacct ttgtataact gcatcacaga agagtagatt ctttcgtgcg
      121 caaatgactc atgtcgataa tgagtgatgg ttctacatga gttcattaag gaattttctc
      181 tatagttatg cttatacata taatatggaa ccgcatacat atcgcatgta attagcagtc
      241 gatagataaa atccagatct tctcctagtc gtaaatcttc atcaaaaaaa agattattct
      301 cttccagaag ttg
//
LOCUS       DAZOZY010000527          312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZOZY010000527 DAZOZY010000000
VERSION     DAZOZY010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.535"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            174..>312
                     /locus_tag="ACHURQ_004567"
     CDS             174..>312
                     /locus_tag="ACHURQ_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR0826113.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
BASE COUNT           91 a           59 c           58 g          104 t
ORIGIN      
        1 agatttgtaa tggctagatt gaaatcagat gtaatccatt agttttatat tttacccatt
       61 tagggctgat ttattactac acacagcagt gcaacatctg tcagtacttc tggtgcttct
      121 attttagagg cagctgtcag gtgtgcgatc aataaaaaaa gcggggtttc atcatgttta
      181 atgaagtcca tagtattcat ggtcatacat tattgttgat cactaaacct tctttgcagg
      241 cgacggctct cttgcagcac cttaaacaat cgctggcaat tacaggaaaa ttacataata
      301 ttcaacgttc tc
//
LOCUS       DAZOZY010000528          310 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.536, whole genome shotgun sequence.
ACCESSION   DAZOZY010000528 DAZOZY010000000
VERSION     DAZOZY010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.536"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>310
                     /locus_tag="ACHURQ_004568"
     CDS             <1..>310
                     /locus_tag="ACHURQ_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826114.1"
                     /translation="KNQTYSITLFENTLVCFMRLYNELRRKVNEEDTPCLECESLEKE
                     FEEMQNDNDLSLFMRTLRTNDTQIYSGVSGGITYTIQYVQNVDIVRVSLPGRGSESIT
                     D"
BASE COUNT          112 a           43 c           61 g           94 t
ORIGIN      
        1 aaaaaatcaa acatattcaa tcacgctttt tgaaaacaca ctggtgtgtt ttatgcggtt
       61 atacaatgaa ctcagacgta aagtaaatga agaggatact ccatgtctgg aatgtgaatc
      121 actagaaaaa gaatttgagg aaatgcagaa tgataatgat ctatcattat ttatgagaac
      181 attgcgtact aatgatacac aaatttattc aggggtttca ggaggtatta catatactat
      241 acaatatgtt caaaatgttg atattgttag agtgtccttg ccgggcagag gttcagagtc
      301 tatcacagat
//
LOCUS       DAZOZY010000529          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZOZY010000529 DAZOZY010000000
VERSION     DAZOZY010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.537"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>308
                     /locus_tag="ACHURQ_004569"
     CDS             <1..>308
                     /locus_tag="ACHURQ_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR0826115.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAZOZY010000530          307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZOZY010000530 DAZOZY010000000
VERSION     DAZOZY010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.538"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>307)
                     /locus_tag="ACHURQ_004570"
     CDS             complement(<1..>307)
                     /locus_tag="ACHURQ_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR0826116.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     "
BASE COUNT           70 a           87 c           72 g           78 t
ORIGIN      
        1 gtattgccgg actgatgcgt gccggatgac ggtaactcca cgctgcctcg accaatagct
       61 ttctggcata gctgtttccg gctttggtaa tgctgccctg atgtctgctt ccacccgatg
      121 aatattctga tggcgtgata ccaaaccatg acatcagttg tcgtggatgt tcaaaacggg
      181 tcagatcacc cagttcagaa attaatccaa ccgcagtaat aaactgaatc cctcgcatgg
      241 cttgcagcgc ctctactacc ggatacagcc tccattcggt aacggcctcc ttcagggcgg
      301 attccag
//
LOCUS       DAZOZY010000531          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.539, whole genome shotgun sequence.
ACCESSION   DAZOZY010000531 DAZOZY010000000
VERSION     DAZOZY010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.539"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHURQ_004571"
     CDS             <1..>301
                     /locus_tag="ACHURQ_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826117.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZOZY010000532          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.540, whole genome shotgun sequence.
ACCESSION   DAZOZY010000532 DAZOZY010000000
VERSION     DAZOZY010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.540"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(<1..>295)
                     /locus_tag="ACHURQ_004572"
     CDS             complement(<1..>295)
                     /locus_tag="ACHURQ_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826118.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIR"
BASE COUNT           89 a           64 c           49 g           93 t
ORIGIN      
        1 tctgatattc ccgttttctt ttacatctac aaaaaactgg cctcttggca tcttgaacgc
       61 aaatgtaaaa ccaacagtat ctgaatcaaa atcatcatct ttatatattg ggatcaaaaa
      121 tgtcatcgtc gctggtgatg cattgtttgc aaaatcaaat acacctatac ttttgccgaa
      181 ttcataagaa gcttttctca atataatcgc accgctggca gaaggggatg ggcacgatat
      241 cctcagttgt ctgaatccat ctgtatccaa gtcaacgtca ttatctgcag atgtt
//
LOCUS       DAZOZY010000533          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.541, whole genome shotgun sequence.
ACCESSION   DAZOZY010000533 DAZOZY010000000
VERSION     DAZOZY010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.541"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>292
                     /locus_tag="ACHURQ_004573"
     CDS             <1..>292
                     /locus_tag="ACHURQ_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR0826119.1"
                     /translation="RGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNG
                     GDWFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEW"
BASE COUNT           78 a           56 c           80 g           78 t
ORIGIN      
        1 atcgtgggcc gcaaagttta cgttgtctgc agttactgga acaacattgg gtttgtgcgg
       61 taagaggcaa tcatgaacag atggcgatgg atgcgctggc atcccagcag atgtctttgt
      121 ggttgatgaa tggcggcgac tggtttattg cgctggcaga taatcaacag aaacaagcga
      181 aaacggcgct ggaaaaatgt cagcatttgc cctttattct tgaagtacac agtcgtaccg
      241 gcaaacatgt tattgctcat gccgattatc cagatgatgt ttatgaatgg ca
//
LOCUS       DAZOZY010000534          291 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.denovo.542, whole genome shotgun sequence.
ACCESSION   DAZOZY010000534 DAZOZY010000000
VERSION     DAZOZY010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.denovo.542"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..>291
                     /locus_tag="ACHURQ_004574"
     CDS             <1..>291
                     /locus_tag="ACHURQ_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR0826120.1"
                     /translation="QGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKN
                     EWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSN"
BASE COUNT           87 a           66 c           80 g           58 t
ORIGIN      
        1 atcagggcag tcattatacg agcaggcagt tccggcagtt attgtggcga taccagatca
       61 gacagagtat gagccggcgc ggaaactgct gggataacag cccaatggaa cgcttcttca
      121 ggagtctgaa gaacgaatgg atgccggtgg tgggttacgt aagcttcagc gaggcagctc
      181 acgccataac ggactatatc gttggatatt acagcgcact aagaccgcac gaatataacg
      241 gtgggttacc cccaaacgaa tcggaaaatc gatactggaa aaactctaac t
//
LOCUS       DAZOZY010000535         9689 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.guided.149, whole genome shotgun sequence.
ACCESSION   DAZOZY010000535 DAZOZY010000000
VERSION     DAZOZY010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9689
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.guided.149"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHURQ_004575"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHURQ_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHURQ_004576"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHURQ_004576"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR0826121.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHURQ_004577"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHURQ_004577"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR0826122.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHURQ_004578"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHURQ_004578"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR0826123.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHURQ_004579"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHURQ_004579"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR0826124.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHURQ_004580"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHURQ_004580"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR0826125.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHURQ_004581"
     CDS             5099..6232
                     /locus_tag="ACHURQ_004581"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR0826126.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6321..6854
                     /gene="blc"
                     /locus_tag="ACHURQ_004582"
     CDS             6321..6854
                     /gene="blc"
                     /locus_tag="ACHURQ_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR0826127.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHURQ_004583"
     CDS             complement(6851..7168)
                     /gene="sugE"
                     /locus_tag="ACHURQ_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR0826128.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHURQ_004584"
     CDS             complement(7343..7489)
                     /gene="ecnB"
                     /locus_tag="ACHURQ_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR0826129.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHURQ_004585"
     CDS             complement(7600..7725)
                     /gene="ecnA"
                     /locus_tag="ACHURQ_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR0826130.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHURQ_004586"
     CDS             complement(7777..8343)
                     /gene="efp"
                     /locus_tag="ACHURQ_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR0826131.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8385..9413
                     /gene="epmB"
                     /locus_tag="ACHURQ_004587"
     CDS             8385..9413
                     /gene="epmB"
                     /locus_tag="ACHURQ_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR0826132.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2462 a         2484 c         2471 g         2272 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
     6361 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtgacagta gtgaataatt
     6421 tcgacgctaa acgctatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
     6481 aacgtggact ggaaaaagtc accgcaacat acagtctgcg tgatgacggc ggcctgaatg
     6541 tcatcaataa aggctataac cctgacagag aaatgtggca gcagagtgaa gggaaagcgt
     6601 actttaccgg cgcaccaaca cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
     6661 gcggttataa cgtcattgca cttgatcggg aataccgcca tgcgctggtt tgcggcccgg
     6721 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
     6781 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
     6841 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
     6901 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
     6961 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt aacgatttca
     7021 tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacagtaata acacttggcg
     7081 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
     7141 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
     7201 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
     7261 gtggcagggg aatttcaact attacctttg tgactatcgc aaaaaaacaa acggcacgac
     7321 ataactgtcg taccgttatt gcttattgct gcgctttcgt tgctgcacca gaaatcgcgt
     7381 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     7441 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     7501 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     7561 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgggagat
     7621 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcga gcggtgttac agcccgtgag
     7681 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     7741 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     7801 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     7861 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     7921 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     7981 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     8041 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     8101 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     8161 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     8221 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     8281 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     8341 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     8401 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     8461 ctggtgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     8521 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     8581 gcaatccgga cgatcctatt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     8641 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     8701 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     8761 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     8821 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     8881 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     8941 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     9001 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     9061 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     9121 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     9181 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     9241 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     9301 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     9361 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     9421 aacagggaag ttaaacatta gcctgatgcg ctacgcttat caggcctaca ttgtgcattg
     9481 aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc gcatccggca
     9541 agagcaacaa acgcaagtac agcaacctgt cattaaactg ggaaatttaa tctttgcccg
     9601 ttgcataacc tttaattatt catatacatt gctgttgtta ctctttatat agccaacacc
     9661 aataattaac ctttcttttc tgtttatat
//
LOCUS       DAZOZY010000536         6482 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.guided.213, whole genome shotgun sequence.
ACCESSION   DAZOZY010000536 DAZOZY010000000
VERSION     DAZOZY010000536.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6482
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.guided.213"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHURQ_004588"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHURQ_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHURQ_004589"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHURQ_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR0826133.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHURQ_004590"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHURQ_004590"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR0826134.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHURQ_004591"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHURQ_004591"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR0826135.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHURQ_004592"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHURQ_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR0826136.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHURQ_004593"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHURQ_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR0826137.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHURQ_004594"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHURQ_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR0826138.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1584 a         1589 c         1659 g         1650 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 ac
//
LOCUS       DAZOZY010000537         6065 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M2008362
            SAMN43277568-rid23486333.guided.224, whole genome shotgun sequence.
ACCESSION   DAZOZY010000537 DAZOZY010000000
VERSION     DAZOZY010000537.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277568
            Sequence Read Archive: SRR30464336
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396687.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396687.1
            Genome Coverage       :: 84x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 08:40:57
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,601
            CDSs (total)                      :: 4,520
            Genes (coding)                    :: 4,252
            CDSs (with protein)               :: 4,252
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 64
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 116 of 268
            Pseudo Genes (incomplete)         :: 97 of 268
            Pseudo Genes (internal stop)      :: 86 of 268
            Pseudo Genes (multiple problems)  :: 30 of 268
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277568-rid23486333.guided.224"
                     /strain="M2008362"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2020-10-30"
                     /collected_by="FSS"
     gene            <1..816
                     /gene="sul2"
                     /locus_tag="ACHURQ_004595"
     CDS             <1..816
                     /gene="sul2"
                     /locus_tag="ACHURQ_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFR0826139.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            903..1205
                     /locus_tag="ACHURQ_004596"
     CDS             903..1205
                     /locus_tag="ACHURQ_004596"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826140.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1381..2874)
                     /locus_tag="ACHURQ_004597"
     CDS             complement(1381..2874)
                     /locus_tag="ACHURQ_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HFR0826141.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKK
                     AGHTHQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPE
                     LTQLTHTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQ
                     AGRKVFTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSE
                     KRLSLTRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPN
                     SRHRALVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAM
                     KVIACIEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
     gene            complement(2918..3307)
                     /locus_tag="ACHURQ_004598"
     CDS             complement(2918..3307)
                     /locus_tag="ACHURQ_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826142.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            3525..3965
                     /locus_tag="ACHURQ_004599"
     CDS             3525..3965
                     /locus_tag="ACHURQ_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFR0826143.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            complement(4688..4837)
                     /locus_tag="ACHURQ_004600"
     CDS             complement(4688..4837)
                     /locus_tag="ACHURQ_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR0826144.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            5408..5566
                     /locus_tag="ACHURQ_004601"
     CDS             5408..5566
                     /locus_tag="ACHURQ_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFR0826145.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDR
                     LRGLEVEA"
BASE COUNT         1442 a         1672 c         1586 g         1365 t
ORIGIN      
        1 atgaataaat cgctcatcat tttcggcatc gtcaacataa cctcggacag tttctccgat
       61 ggaggccggt atctggcgcc agacgcagcc attgcgcagg cgcgtaagct gatggccgag
      121 ggggcagatg tgatcgacct cggtccggca tccagcaatc ccgacgccgc gcctgtttcg
      181 tccgacacag aaatcgcgcg tatcgcgccg gtgctggacg cgctcaaggc agatggcatt
      241 cccgtctcgc tcgacagtta tcaacccgcg acgcaagcct atgccttgtc gcgtggtgtg
      301 gcctatctca atgatattcg cggttttcca gacgctgcgt tctatccgca attggcgaaa
      361 tcatctgcca aactcgtcgt tatgcattcg gtgcaagacg ggcaggcaga tcggcgcgag
      421 gcacccgctg gcgacatcat ggatcacatt gcggcgttct ttgacgcgcg catcgcggcg
      481 ctgacgggtg ccggtatcaa acgcaaccgc cttgtccttg atcccggcat ggggtttttt
      541 ctgggggctg ctcccgaaac ctcgctctcg gtgctggcgc ggttcgatga attgcggctg
      601 cgcttcgatt tgccggtgct tctgtctgtt tcgcgcaaat cctttctgcg cgcgctcaca
      661 ggccgtggtc cgggggatgt cggggccgcg acactcgctg cagagcttgc cgccgccgca
      721 ggtggagctg acttcatccg cacacacgag ccgcgcccct tgcgcgacgg gctggcggta
      781 ttggcggcgc tgaaagaaac cgcaagaatt cgttaactgc acattcggga tatttctcta
      841 tattcgcggt tcagcaggca tgtccccttt gagggcgacc cgacgacagg ataatcgacc
      901 ttatggtgcg caaatatttc ggcacagacg gtattcgtgg caaagccaac gaaggcgcga
      961 tgacggcgga aaccgccttg cgcgtcggca tggcggctgg ccgtgtcttt cgtcgcggtg
     1021 accaccgcca tcgtgtcgtg atcggcaagg atacgcgcct gtcgggctat atgcttgaac
     1081 ccgcgctcac agccggtttc acctcgatgg gcatggacgt attccttttt ggcccgctgc
     1141 cgacaacgta taggaagaat aaacgccctt ttcacccaag tccaacagct ttggaccgca
     1201 gttgactctt tcgacacccc tgcgatgcaa cccaatccgg ctgacgggga gccagcaacg
     1261 ctgaaaattt accctcctct ttcccactag cggctccttt tccgacaacc agcacggcgg
     1321 atccctgccg cggcgctgtg aacgcagcat tttgattggt atcgttggcc ttcaggctcg
     1381 tcagtcaaac agacccagga gcagctcagc cggtggcgcc cggctttcgg gtaacgccct
     1441 ggtcccgctg gtttcggctt tgtgcttcag gtgatcaagg atctgcttga tcactatagg
     1501 gtcttcaatg caggcgatga ctttcatggc gccgccgcag ccgctgcagg tctcgatgtc
     1561 gatattgaaa acacgcttga gccgttgcgc ccatgtcatc gacgctcgcc gttgtgctgg
     1621 tgttgccggt tcatcagcca ccctgacctt gttgcccctg ccccgttttg ccggcgtgac
     1681 caacgcccgg tgccgactgt tgggtgcgaa caccccgtgg aagcgggtta ggttgactct
     1741 gggcttcggt accagggcgg ccagccttgc aatgaaatcc aatggttcga aaatgacgtg
     1801 cgtggtgccg tcccggtacg gcgtcttgag ctggtagcgc acgttgccgc ctcgtgttaa
     1861 cgacagccgc ttctcggata ccgccgggcg gctgatgtac cggcacagcc gttcgagctt
     1921 cttgcgttca tcggccctgg ccgccacgcc ggcgtgcagg ctggacccgg ctaccttgcc
     1981 aatcccgtca ccgaacggat caccactggt cggcagagtt tgcaaagtga acacctttcg
     2041 ccccgcctgt gaaccgacag cgatacggta agtgatcgag tgccccagca ggggtgtcat
     2101 cgggtcgtca tccaccgcat ccgaggccag atagctgttt tcgacatccc gttccagcag
     2161 gccttgccgt tccagatagc gacccacccg gtgggcgatg gtgtgcgtca gctgggtgag
     2221 ctctgggctg gtcggcgcct tgacccagcg gaaacgcgct gagccgtggg attgctcgac
     2281 atacacaccg tcgagaaaca gcatgtggaa gtgaacattc agattgagcg ccgatccaaa
     2341 acgctggatc agggtgaccg cgcccgtctt ggccacttgg tgggtatggc ccgctttctt
     2401 gaccaggtgc gtggcaatga cgcggtaaac gatgcccagc acccacccca tgatctcggg
     2461 ccggctggca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     2521 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     2581 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     2641 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     2701 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     2761 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     2821 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     2881 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     2941 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     3001 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     3061 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     3121 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     3181 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     3241 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     3301 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     3361 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     3421 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     3481 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     3541 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     3601 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     3661 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     3721 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     3781 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     3841 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     3901 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     3961 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     4021 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     4081 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     4141 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcgttcttc ccatccgaaa
     4201 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     4261 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     4321 acgtatgagc tgaaggaatg atgtaaaagt gatgtatgaa ctgaaggagc gatgtaattc
     4381 tgtagtcagg aagaataacc cgttcatgca ggcatgaacg atacagtgag gaacgctttt
     4441 tttgtcacga tctcccctta acctgcgtaa atcacccgcc atggttgatt atttttcaac
     4501 ctcaaaatgg tcatacacct aacgcagccg cgattttcat cgcaactgca tgaattaaaa
     4561 gaaaaaaaag gaatatttcg ttttcacgag taatacccgg gtattaaaaa tccactgtaa
     4621 ttacatgaaa ataaagaaat aaaaggtaat accggagtat taccacgtac gcgggcaagc
     4681 gaaaaagcta taacgcttcc cggccgacag gcgacccaaa atccacaggg gcatgcaggt
     4741 tctcttcagt aacctgcgcc attaaatcaa aaaggtcata ttcgccggga agctgcgtat
     4801 cagtcgaatc cagttgttca tcatattcag atggcatggg gtgatatcca gtagctgaga
     4861 ggttgttagc gggtttcggg gcgcagccct gaaccagtca tgtagcgcta gcggagtgta
     4921 tactggctta ctatgttgcc acgattgcga aaatcagtga agtgcttcat gtggcaggag
     4981 aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac aggagatatt ccgcttcctc
     5041 gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg cttacggacg
     5101 gggacggaaa tttcctggaa gataccagga agacacttag caaagaagag ataaggccac
     5161 ggcgaagccg tttttccata ggctccgccc ccctgacaag catcgcaaaa tctgacgctc
     5221 aaatcagtgg tggcgaaacc cgacaggact ataaagatcc caggcgtttc cccctggtag
     5281 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     5341 gccgcggttt ctcattccac gcctgacact cagttccgtg aaggcagttc gctccaagct
     5401 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttgtccg gtaactatca
     5461 acttgagtcc aacccggaaa gacacgacaa aacgccactg gcagcagcca ttagtaacag
     5521 gagtgcacga gaagatagat taagaggtct tgaagtagag gcctgactgc ggctacactg
     5581 aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt accgggttaa gcagttcccc
     5641 aactgactta accttcgaac aaaccgcctc cccaggcggt tttttcgttt acagagcaag
     5701 agattacgat tcattgaaaa ggatctcaag aagatccttt gtagattact atcaattaat
     5761 cttccgaaca tgacaccagt cactgttgat tacggttaaa ccgctccgta acaggtttcc
     5821 ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga taaatatatc tacttattga
     5881 gagatgatat atttatttcc aaaatgctac agatacatca tcatgaaacg gatcaccgca
     5941 aacaggttac tcgcctgggg attccctttc gacccgagca tccgtatgag actcatgctc
     6001 gattattatt attatagaag cccccatgaa taaatcgctc atcattttcg gcatcgtcaa
     6061 cataa
//
